LOCUS       DANKAZ010000001       128947 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000001 DANKAZ010000000
VERSION     DANKAZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 128947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 128947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..128947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(12..1535)
                     /locus_tag="PWY67_000001"
     CDS             complement(12..1535)
                     /locus_tag="PWY67_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2056318.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="PWY67_000002"
     CDS             1787..2968
                     /locus_tag="PWY67_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2056319.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="PWY67_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="PWY67_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL2056320.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="PWY67_000004"
     CDS             complement(5053..7764)
                     /locus_tag="PWY67_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2056321.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="PWY67_000005"
     CDS             complement(7774..8475)
                     /locus_tag="PWY67_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2056322.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="PWY67_000006"
     CDS             8661..9260
                     /locus_tag="PWY67_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2056323.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="PWY67_000007"
     CDS             9341..10354
                     /locus_tag="PWY67_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2056324.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="PWY67_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="PWY67_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL2056325.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="PWY67_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="PWY67_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL2056326.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="PWY67_000010"
     CDS             11802..13175
                     /locus_tag="PWY67_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2056327.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="PWY67_000011"
     CDS             13532..15676
                     /locus_tag="PWY67_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2056328.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="PWY67_000012"
     CDS             complement(15728..16768)
                     /locus_tag="PWY67_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2056329.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="PWY67_000013"
     CDS             complement(17045..18847)
                     /locus_tag="PWY67_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2056330.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="PWY67_000014"
     CDS             complement(18861..19613)
                     /locus_tag="PWY67_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2056331.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="PWY67_000015"
     CDS             complement(19622..19984)
                     /locus_tag="PWY67_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2056332.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="PWY67_000016"
     CDS             complement(20160..21032)
                     /locus_tag="PWY67_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2056333.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="PWY67_000017"
     CDS             complement(21058..21570)
                     /locus_tag="PWY67_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2056334.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="PWY67_000018"
     CDS             complement(21708..22499)
                     /locus_tag="PWY67_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL2056335.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="PWY67_000019"
     CDS             complement(22554..23162)
                     /locus_tag="PWY67_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL2056336.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="PWY67_000020"
     CDS             complement(23155..23472)
                     /locus_tag="PWY67_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2056337.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="PWY67_000021"
     CDS             complement(23628..24356)
                     /locus_tag="PWY67_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL2056338.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="PWY67_000022"
     CDS             24515..25396
                     /locus_tag="PWY67_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2056339.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="PWY67_000023"
     CDS             complement(25633..26676)
                     /locus_tag="PWY67_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2056340.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="PWY67_000024"
     CDS             complement(26679..27347)
                     /locus_tag="PWY67_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2056341.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="PWY67_000025"
     CDS             27489..29825
                     /locus_tag="PWY67_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2056342.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="PWY67_000026"
     CDS             complement(29940..30251)
                     /locus_tag="PWY67_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056343.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="PWY67_000027"
     CDS             30432..31907
                     /locus_tag="PWY67_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL2056344.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="PWY67_000028"
     CDS             31954..32538
                     /locus_tag="PWY67_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL2056345.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="PWY67_000029"
     CDS             32540..32959
                     /locus_tag="PWY67_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL2056346.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="PWY67_000030"
     CDS             complement(33240..33524)
                     /locus_tag="PWY67_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL2056347.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="PWY67_000031"
     CDS             complement(33876..34586)
                     /locus_tag="PWY67_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL2056348.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="PWY67_000032"
     CDS             complement(34583..36055)
                     /locus_tag="PWY67_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2056349.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="PWY67_000033"
     CDS             36193..36666
                     /locus_tag="PWY67_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2056350.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="PWY67_000034"
     CDS             complement(36819..37370)
                     /locus_tag="PWY67_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2056351.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="PWY67_000035"
     CDS             37475..38005
                     /locus_tag="PWY67_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL2056352.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="PWY67_000036"
     CDS             complement(38488..38931)
                     /locus_tag="PWY67_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2056353.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="PWY67_000037"
     CDS             complement(38947..40107)
                     /locus_tag="PWY67_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2056354.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="PWY67_000038"
     CDS             40614..42314
                     /locus_tag="PWY67_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056355.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="PWY67_000039"
     CDS             complement(42416..43249)
                     /locus_tag="PWY67_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL2056356.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="PWY67_000040"
     CDS             complement(43553..45718)
                     /locus_tag="PWY67_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2056357.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="PWY67_000041"
     CDS             45978..48272
                     /locus_tag="PWY67_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2056358.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="PWY67_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="PWY67_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2056359.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="PWY67_000043"
     CDS             48923..49930
                     /locus_tag="PWY67_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2056360.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="PWY67_000044"
     CDS             49952..50971
                     /locus_tag="PWY67_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2056361.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="PWY67_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="PWY67_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2056362.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="PWY67_000046"
     CDS             complement(52729..54150)
                     /locus_tag="PWY67_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2056363.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="PWY67_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="PWY67_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2056364.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="PWY67_000048"
     CDS             complement(56561..57817)
                     /locus_tag="PWY67_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL2056365.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="PWY67_000049"
     CDS             58397..59698
                     /locus_tag="PWY67_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2056366.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="PWY67_000050"
     CDS             complement(59767..60366)
                     /locus_tag="PWY67_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2056367.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="PWY67_000051"
     CDS             60620..61501
                     /locus_tag="PWY67_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2056368.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="PWY67_000052"
     CDS             61514..63124
                     /locus_tag="PWY67_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL2056369.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="PWY67_000053"
     CDS             complement(63201..63860)
                     /locus_tag="PWY67_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2056370.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="PWY67_000054"
     CDS             complement(64143..64808)
                     /locus_tag="PWY67_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL2056371.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="PWY67_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="PWY67_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL2056372.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="PWY67_000056"
     CDS             complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="PWY67_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL2056373.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="PWY67_000057"
     CDS             complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="PWY67_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL2056374.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="PWY67_000058"
     CDS             67122..67442
                     /locus_tag="PWY67_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL2056375.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="PWY67_000059"
     CDS             complement(67554..68423)
                     /locus_tag="PWY67_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2056376.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69822)
                     /locus_tag="PWY67_000060"
                     /pseudo
     CDS             complement(68519..69822)
                     /locus_tag="PWY67_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(69819..70328)
                     /locus_tag="PWY67_000061"
     CDS             complement(69819..70328)
                     /locus_tag="PWY67_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056377.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="PWY67_000062"
     CDS             complement(70503..72638)
                     /locus_tag="PWY67_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2056378.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="PWY67_000063"
     CDS             complement(72721..74376)
                     /locus_tag="PWY67_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2056379.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="PWY67_000064"
     CDS             complement(74378..74692)
                     /locus_tag="PWY67_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2056380.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="PWY67_000065"
     CDS             74862..75122
                     /locus_tag="PWY67_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL2056381.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="PWY67_000066"
     CDS             75136..75450
                     /locus_tag="PWY67_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL2056382.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="PWY67_000067"
     CDS             complement(76180..76734)
                     /locus_tag="PWY67_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL2056383.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="PWY67_000068"
     CDS             complement(76860..78788)
                     /locus_tag="PWY67_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL2056384.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="PWY67_000069"
     CDS             complement(79094..79810)
                     /locus_tag="PWY67_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2056385.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="PWY67_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="PWY67_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL2056386.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="PWY67_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="PWY67_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL2056387.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="PWY67_000072"
     CDS             complement(83280..84740)
                     /locus_tag="PWY67_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2056388.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="PWY67_000073"
     CDS             complement(84730..86025)
                     /locus_tag="PWY67_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2056389.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="PWY67_000074"
     CDS             complement(86357..87034)
                     /locus_tag="PWY67_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL2056390.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="PWY67_000075"
     CDS             complement(87064..88155)
                     /locus_tag="PWY67_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL2056391.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="PWY67_000076"
     CDS             complement(88256..88690)
                     /locus_tag="PWY67_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2056392.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="PWY67_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="PWY67_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2056393.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="PWY67_000078"
     CDS             complement(90728..92623)
                     /locus_tag="PWY67_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2056394.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="PWY67_000079"
     CDS             complement(92681..94456)
                     /locus_tag="PWY67_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2056395.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="PWY67_000080"
     CDS             complement(94506..96002)
                     /locus_tag="PWY67_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2056396.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="PWY67_000081"
     CDS             complement(96196..97818)
                     /locus_tag="PWY67_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2056397.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="PWY67_000082"
     CDS             complement(98267..99580)
                     /locus_tag="PWY67_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2056398.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="PWY67_000083"
     CDS             complement(99825..100049)
                     /locus_tag="PWY67_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056399.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100491)
                     /locus_tag="PWY67_000084"
     CDS             complement(100069..100491)
                     /locus_tag="PWY67_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL2056400.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="PWY67_000085"
     CDS             complement(100635..101345)
                     /locus_tag="PWY67_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056401.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="PWY67_000086"
     CDS             complement(101363..101860)
                     /locus_tag="PWY67_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2056402.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="PWY67_000087"
     CDS             complement(101897..102349)
                     /locus_tag="PWY67_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2056403.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="PWY67_000088"
     CDS             complement(102484..103089)
                     /locus_tag="PWY67_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2056404.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="PWY67_000089"
     CDS             complement(103539..104159)
                     /locus_tag="PWY67_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2056405.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="PWY67_000090"
     CDS             complement(104161..105030)
                     /locus_tag="PWY67_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL2056406.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="PWY67_000091"
     CDS             complement(105053..105460)
                     /locus_tag="PWY67_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2056407.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="PWY67_000092"
     CDS             complement(105479..106768)
                     /locus_tag="PWY67_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2056408.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="PWY67_000093"
     CDS             complement(106795..107097)
                     /locus_tag="PWY67_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2056409.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="PWY67_000094"
     CDS             complement(107122..109215)
                     /locus_tag="PWY67_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2056410.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="PWY67_000095"
     CDS             complement(109704..110468)
                     /locus_tag="PWY67_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDL2056411.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="PWY67_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="PWY67_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDL2056412.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="PWY67_000097"
     CDS             complement(111473..112549)
                     /locus_tag="PWY67_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL2056413.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="PWY67_000098"
     CDS             complement(112573..113166)
                     /locus_tag="PWY67_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL2056414.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="PWY67_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="PWY67_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL2056415.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="PWY67_000100"
     CDS             complement(114836..115135)
                     /locus_tag="PWY67_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL2056416.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="PWY67_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="PWY67_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDL2056417.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="PWY67_000102"
     CDS             complement(116890..117801)
                     /locus_tag="PWY67_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2056418.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="PWY67_000103"
     CDS             117921..118778
                     /locus_tag="PWY67_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2056419.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118965..119327)
                     /locus_tag="PWY67_000104"
     CDS             complement(118965..119327)
                     /locus_tag="PWY67_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL2056420.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            119673..121112
                     /locus_tag="PWY67_000105"
     CDS             119673..121112
                     /locus_tag="PWY67_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2056421.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121119..123671)
                     /locus_tag="PWY67_000106"
     CDS             complement(121119..123671)
                     /locus_tag="PWY67_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL2056422.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123785..125116)
                     /locus_tag="PWY67_000107"
     CDS             complement(123785..125116)
                     /locus_tag="PWY67_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2056423.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125082..126560)
                     /locus_tag="PWY67_000108"
     CDS             complement(125082..126560)
                     /locus_tag="PWY67_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2056424.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="PWY67_000109"
     CDS             complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="PWY67_000109"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2056425.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        37741 a        26961 c        22546 g        41699 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggaa ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
   118801 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
   118861 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   118921 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
   118981 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
   119041 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
   119101 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
   119161 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
   119221 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
   119281 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
   119341 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
   119401 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
   119461 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
   119521 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
   119581 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
   119641 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
   119701 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
   119761 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
   119821 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
   119881 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
   119941 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
   120001 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
   120061 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
   120121 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
   120181 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
   120241 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
   120301 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
   120361 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
   120421 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
   120481 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
   120541 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
   120601 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
   120661 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
   120721 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
   120781 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
   120841 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
   120901 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
   120961 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
   121021 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
   121081 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
   121141 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
   121201 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
   121261 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
   121321 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
   121381 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
   121441 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
   121501 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
   121561 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
   121621 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
   121681 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
   121741 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
   121801 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
   121861 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
   121921 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
   121981 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
   122041 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
   122101 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
   122161 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
   122221 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
   122281 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
   122341 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
   122401 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
   122461 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
   122521 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
   122581 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
   122641 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
   122701 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
   122761 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
   122821 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
   122881 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
   122941 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
   123001 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
   123061 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
   123121 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
   123181 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
   123241 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
   123301 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
   123361 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
   123421 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
   123481 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
   123541 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
   123601 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
   123661 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
   123721 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
   123781 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
   123841 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
   123901 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
   123961 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
   124021 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
   124081 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
   124141 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
   124201 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
   124261 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
   124321 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
   124381 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
   124441 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
   124501 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
   124561 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
   124621 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
   124681 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
   124741 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
   124801 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
   124861 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
   124921 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
   124981 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
   125041 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
   125101 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
   125161 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
   125221 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
   125281 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
   125341 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
   125401 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
   125461 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
   125521 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
   125581 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
   125641 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
   125701 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
   125761 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
   125821 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
   125881 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
   125941 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
   126001 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
   126061 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
   126121 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
   126181 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
   126241 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
   126301 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
   126361 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
   126421 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
   126481 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
   126541 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
   126601 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
   126661 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
   126721 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
   126781 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
   126841 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
   126901 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
   126961 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
   127021 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
   127081 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
   127141 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
   127201 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
   127261 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
   127321 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
   127381 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
   127441 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
   127501 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
   127561 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
   127621 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
   127681 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
   127741 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
   127801 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
   127861 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
   127921 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
   127981 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
   128041 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
   128101 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
   128161 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
   128221 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
   128281 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
   128341 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
   128401 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
   128461 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
   128521 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
   128581 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
   128641 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
   128701 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
   128761 acctcttcac tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgtag
   128821 atttttacaa acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg
   128881 aacaattttt caataaatca gtcttctaaa atagataacc taaaaaaagc gaacaaacaa
   128941 ataaaat
//
LOCUS       DANKAZ010000002        64260 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000002 DANKAZ010000000
VERSION     DANKAZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..205)
                     /locus_tag="PWY67_000110"
     CDS             complement(<1..205)
                     /locus_tag="PWY67_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2056426.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLS"
     gene            399..692
                     /locus_tag="PWY67_000111"
     CDS             399..692
                     /locus_tag="PWY67_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056427.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            736..1047
                     /locus_tag="PWY67_000112"
     CDS             736..1047
                     /locus_tag="PWY67_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056428.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            1044..1668
                     /locus_tag="PWY67_000113"
                     /pseudo
     CDS             1044..1668
                     /locus_tag="PWY67_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            1809..1967
                     /locus_tag="PWY67_000114"
     CDS             1809..1967
                     /locus_tag="PWY67_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056429.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            1968..2330
                     /locus_tag="PWY67_000115"
     CDS             1968..2330
                     /locus_tag="PWY67_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056430.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            2367..3215
                     /locus_tag="PWY67_000116"
     CDS             2367..3215
                     /locus_tag="PWY67_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDL2056431.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            3205..4680
                     /locus_tag="PWY67_000117"
     CDS             3205..4680
                     /locus_tag="PWY67_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL2056432.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            4946..5353
                     /locus_tag="PWY67_000118"
     CDS             4946..5353
                     /locus_tag="PWY67_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDL2056433.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            5356..5571
                     /locus_tag="PWY67_000119"
     CDS             5356..5571
                     /locus_tag="PWY67_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056434.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            5575..5955
                     /locus_tag="PWY67_000120"
     CDS             5575..5955
                     /locus_tag="PWY67_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2056435.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            6089..6244
                     /locus_tag="PWY67_000121"
     CDS             6089..6244
                     /locus_tag="PWY67_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDL2056436.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            6313..6786
                     /locus_tag="PWY67_000122"
     CDS             6313..6786
                     /locus_tag="PWY67_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL2056437.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            6783..8477
                     /locus_tag="PWY67_000123"
     CDS             6783..8477
                     /locus_tag="PWY67_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2056438.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            8443..8628
                     /locus_tag="PWY67_000124"
     CDS             8443..8628
                     /locus_tag="PWY67_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056439.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            8632..9807
                     /locus_tag="PWY67_000125"
     CDS             8632..9807
                     /locus_tag="PWY67_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2056440.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            9800..11323
                     /locus_tag="PWY67_000126"
     CDS             9800..11323
                     /locus_tag="PWY67_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2056441.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            11379..11663
                     /locus_tag="PWY67_000127"
     CDS             11379..11663
                     /locus_tag="PWY67_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2056442.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            11650..11985
                     /locus_tag="PWY67_000128"
     CDS             11650..11985
                     /locus_tag="PWY67_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2056443.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            12280..12906
                     /locus_tag="PWY67_000129"
     CDS             12280..12906
                     /locus_tag="PWY67_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056444.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            13648..14485
                     /locus_tag="PWY67_000130"
                     /pseudo
     CDS             13648..14485
                     /locus_tag="PWY67_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            14550..14984
                     /locus_tag="PWY67_000131"
     CDS             14550..14984
                     /locus_tag="PWY67_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2056445.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(15052..16374)
                     /locus_tag="PWY67_000132"
     CDS             complement(15052..16374)
                     /locus_tag="PWY67_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL2056446.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(16528..18168)
                     /locus_tag="PWY67_000133"
     CDS             complement(16528..18168)
                     /locus_tag="PWY67_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL2056447.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            18498..18968
                     /locus_tag="PWY67_000134"
     CDS             18498..18968
                     /locus_tag="PWY67_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL2056448.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            19597..20700
                     /locus_tag="PWY67_000135"
     CDS             19597..20700
                     /locus_tag="PWY67_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2056449.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            20984..21664
                     /locus_tag="PWY67_000136"
     CDS             20984..21664
                     /locus_tag="PWY67_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2056450.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            21754..22788
                     /locus_tag="PWY67_000137"
     CDS             21754..22788
                     /locus_tag="PWY67_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2056451.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            22802..23137
                     /locus_tag="PWY67_000138"
     CDS             22802..23137
                     /locus_tag="PWY67_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056452.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            23121..25601
                     /locus_tag="PWY67_000139"
     CDS             23121..25601
                     /locus_tag="PWY67_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056453.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            25657..27216
                     /locus_tag="PWY67_000140"
     CDS             25657..27216
                     /locus_tag="PWY67_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2056454.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            27213..28577
                     /locus_tag="PWY67_000141"
     CDS             27213..28577
                     /locus_tag="PWY67_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2056455.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            28748..29818
                     /locus_tag="PWY67_000142"
     CDS             28748..29818
                     /locus_tag="PWY67_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2056456.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            29833..30501
                     /locus_tag="PWY67_000143"
     CDS             29833..30501
                     /locus_tag="PWY67_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2056457.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            30521..31099
                     /locus_tag="PWY67_000144"
     CDS             30521..31099
                     /locus_tag="PWY67_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2056458.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            31502..32413
                     /locus_tag="PWY67_000145"
     CDS             31502..32413
                     /locus_tag="PWY67_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2056459.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(32669..33586)
                     /locus_tag="PWY67_000146"
     CDS             complement(32669..33586)
                     /locus_tag="PWY67_000146"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL2056460.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            33788..34225
                     /gene="zapA"
                     /locus_tag="PWY67_000147"
     CDS             33788..34225
                     /gene="zapA"
                     /locus_tag="PWY67_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL2056461.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            34283..34830
                     /locus_tag="PWY67_000148"
                     /pseudo
     CDS             34283..34830
                     /locus_tag="PWY67_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            34945..37305
                     /locus_tag="PWY67_000149"
     CDS             34945..37305
                     /locus_tag="PWY67_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL2056462.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            37392..37706
                     /gene="trxA"
                     /locus_tag="PWY67_000150"
     CDS             37392..37706
                     /gene="trxA"
                     /locus_tag="PWY67_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL2056463.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            37888..39666
                     /gene="uvrC"
                     /locus_tag="PWY67_000151"
     CDS             37888..39666
                     /gene="uvrC"
                     /locus_tag="PWY67_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL2056464.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            39811..40710
                     /locus_tag="PWY67_000152"
     CDS             39811..40710
                     /locus_tag="PWY67_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2056465.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            40703..41965
                     /locus_tag="PWY67_000153"
     CDS             40703..41965
                     /locus_tag="PWY67_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2056466.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            42128..44026
                     /locus_tag="PWY67_000154"
     CDS             42128..44026
                     /locus_tag="PWY67_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL2056467.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(44325..45044)
                     /locus_tag="PWY67_000155"
     CDS             complement(44325..45044)
                     /locus_tag="PWY67_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL2056468.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            45381..45713
                     /locus_tag="PWY67_000156"
     CDS             45381..45713
                     /locus_tag="PWY67_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056469.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            45700..47703
                     /locus_tag="PWY67_000157"
     CDS             45700..47703
                     /locus_tag="PWY67_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL2056470.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            47722..48192
                     /locus_tag="PWY67_000158"
     CDS             47722..48192
                     /locus_tag="PWY67_000158"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL2056471.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            48213..48797
                     /locus_tag="PWY67_000159"
     CDS             48213..48797
                     /locus_tag="PWY67_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL2056472.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            48812..49798
                     /locus_tag="PWY67_000160"
     CDS             48812..49798
                     /locus_tag="PWY67_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL2056473.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            49788..50099
                     /locus_tag="PWY67_000161"
     CDS             49788..50099
                     /locus_tag="PWY67_000161"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL2056474.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            50121..51902
                     /locus_tag="PWY67_000162"
     CDS             50121..51902
                     /locus_tag="PWY67_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL2056475.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            51895..53271
                     /locus_tag="PWY67_000163"
     CDS             51895..53271
                     /locus_tag="PWY67_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL2056476.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            53278..53913
                     /locus_tag="PWY67_000164"
     CDS             53278..53913
                     /locus_tag="PWY67_000164"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL2056477.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            53943..55298
                     /locus_tag="PWY67_000165"
     CDS             53943..55298
                     /locus_tag="PWY67_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL2056478.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(55490..56884)
                     /locus_tag="PWY67_000166"
     CDS             complement(55490..56884)
                     /locus_tag="PWY67_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2056479.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            56976..57947
                     /locus_tag="PWY67_000167"
     CDS             56976..57947
                     /locus_tag="PWY67_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL2056480.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            57944..58726
                     /locus_tag="PWY67_000168"
     CDS             57944..58726
                     /locus_tag="PWY67_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL2056481.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(58895..59266)
                     /locus_tag="PWY67_000169"
     CDS             complement(58895..59266)
                     /locus_tag="PWY67_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2056482.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            59400..59843
                     /locus_tag="PWY67_000170"
     CDS             59400..59843
                     /locus_tag="PWY67_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL2056483.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(59886..60695)
                     /gene="yidA"
                     /locus_tag="PWY67_000171"
     CDS             complement(59886..60695)
                     /gene="yidA"
                     /locus_tag="PWY67_000171"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL2056484.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(60714..62081)
                     /locus_tag="PWY67_000172"
     CDS             complement(60714..62081)
                     /locus_tag="PWY67_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2056485.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(62065..62895)
                     /locus_tag="PWY67_000173"
     CDS             complement(62065..62895)
                     /locus_tag="PWY67_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL2056486.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            63058..63495
                     /locus_tag="PWY67_000174"
     CDS             63058..63495
                     /locus_tag="PWY67_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL2056487.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            63571..64191
                     /locus_tag="PWY67_000175"
     CDS             63571..64191
                     /locus_tag="PWY67_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL2056488.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        21373 a        10925 c        13534 g        18428 t
ORIGIN      
        1 ttgaaagttg tttaagcctg gtgttattgg gaagattata gccattttcc caattactaa
       61 cggcaccttt actggccggt ggggataatt tacctccaag ttgttccatt gagagtccct
      121 tttcaattct agccagtcta attctttgtc ctgtctccac tgcttcgggg tatttttttc
      181 tagccacatt ttcaccacca ttcatatatt ttctctacta tatcacgatt taaatatgtg
      241 tgaaacataa aagttcaaaa aagtatgtta aaattcttgc aaatgaaaag gtatgatggt
      301 atattgagtt tataagaggt atagaaatgt attcaagagc ctcttgcatt tttttaaaat
      361 ctttgtatag aaatgtatgc taacgagggg ggataaatat ggaattaaaa attgacaaac
      421 tcacgcttga tgatccagct tttaaaatta ttgacgatcg agttgttcaa caagtagagc
      481 aaactcaagg aacgttactt tcattattag aaaaaaagta ccagtttcca aaatatatga
      541 acaaggcaca agctgctaag tatatgaacg tttcatacaa tactatgatg cagaaatatg
      601 ttcctaacgg tttaaagttg attatagttg atggagtcgt aagaatttca caagatgaat
      661 gtgatcgctt tatggaagaa taccaaaaat aaacgcaagc cgtgggggca aagggattaa
      721 taggaggaaa taataatgga atcgacacaa aataaagcaa tcgaaaaagt attatcaaca
      781 attcttacag aagacacagc ggcagaatta gcaaacttgg aaggcaaagc gctagaagaa
      841 acgtttgaat gtttgtacga acaaatggac tatcaaaaat tgctaccaca agaaccaacg
      901 gtaagcggag tattgcgtgg cttgaacgat ttagtacaag cagagtttaa agaacgactt
      961 tctattgaag agtatcaaga aattttgtat cagcaagttg agttgttagc gagcttgcta
     1021 gggcttgaac tggaggacgt tgaatgaaaa ctgaaatttg gaacggacac attataagat
     1081 ttgtcgatat taatgatgaa tggtgggcag tggcgaaaga tgttgcggaa gcgttaggtc
     1141 ttaaacaggt tactagagcc attcactctt tgcctaaaga tggggttact actagtaagg
     1201 tcatcgattc attgggcaga acacaagatg taaacatcat caacgaaaaa aaatatttac
     1261 cgcatggcat tcaaaagccg taaaaaagaa gccgaggcat ttcaagactg gattttcgac
     1321 atcatcaaag aactacgcca gtccacagga cttgaaggct ttcaagtatt ccgcatgcta
     1381 gacaaagaac accaaaaaga agcaatgact aaattaagcc acgctattac tgaaccaaaa
     1441 ccagtagact acattaaagc gaatgtgata gccaataaag ctgtatcaac tatttatggt
     1501 cattccaaaa tggtaaaaaa gaaagatatg actcctgaaa tgttggttga tcgtgaacct
     1561 attttagatg agacagtaga attaatgact gttaaagaaa aatatgggtt gcagttttca
     1621 gtgagtgaga aaatttataa tcgttctgct gaattgcaga caacttagga gcattaacat
     1681 gatcgagaac aaagaaaaag ccttatccat aacagtcttg gcggacagga taaaggcaac
     1741 ttaattggga taaaacccga tattctttgt tccgattata ccatattaaa aaatcggagg
     1801 gaaccattat gaaacgaaac ttaattatag gaagcatttt aatcggcgga attttcggct
     1861 tattaccatt aattgcaaaa atagctatct ttgcaggatt agcgttatat ctcttcactg
     1921 cttatgacga gtacgactat caattaagaa ctggagggca taagtagatg tacaaaacaa
     1981 aactattaaa ccaactcgat agccttgaat tagaggaaat taatcaaggg atcgctgaac
     2041 tagaaaataa tatcgggaaa acctactttg gaaattcatt caatgaaaaa ctaacagttt
     2101 tgtatgtact caaaaaacat gcagaacaca aaataatttg tcgagaaatc aacgagctaa
     2161 aaaatcagat tttaaccgca tggttaaaca ttactgatat gcaagaagca agggtgaaaa
     2221 catttaatac atgggtaaag tatcagaatc aattaaaagg tgctgaattt gttcgggatg
     2281 ggttgaaata cgaacttgag cagttgaagc tcatggaggt gtctgaatga tttataccag
     2341 tattttgaaa aaggaggaga ggtttattgg caaataatta tcaagcagca acggaattac
     2401 aaaagtcggg atggtcaatt tatcccttat ctcctggaac taaaattcca ataaaaggct
     2461 ctagtggttg gaatgatgca actaacgatt ctaatcaaat taatgaatgg tggacggaag
     2521 atgcaactag aaatattggg ttaatgcttg aagatcatca aatggtggta gttgacctag
     2581 atcaacattc tgaaacacga aacggcgttg agaattataa aaaacttgct tcaatgtatg
     2641 atccatttcc ttctacttat acggaaatca caccaggtaa aggaatccat ttcttcttta
     2701 aaattcctga tggtgtaatt atccaacaag aaacaggtgc atttgctgat atcttcgggc
     2761 gtgatgataa aggtaaaagt ttaagcggga ttgatattat cactaaagga gttccaatag
     2821 cgccaactat gattatttca agaagtgttg ggagagcata tgaacctatt gaaaacgtat
     2881 ctctaaataa tattgcacca gctcccgatt ggctagtgaa cctactgcta aaaaaaccgc
     2941 aagaaataaa gactcaatac cgacccgtag caaaaacagg gacagcaaaa aaattagata
     3001 tgattgttca aggcgctggc gaaggtagta gaaacgatca tttaactaag ttatgcggtt
     3061 ggttactatg gcacggtgta gataatcaaa ctttagcaga attgatttat acagcaaatg
     3121 cgtacaatga accaccgcta aaagataaag aagtgaatca aattattcga tcgatgatta
     3181 aaaaagattt gagggggaaa gctcatggct cataagcata aattagatag tattttagac
     3241 tttcccgaag ccagcgagcg tgaagacaat atcattgaat tgaaaacctg gatgtcacga
     3301 ttaagatgca ataaagacga tcagattaaa agtaacagtg ttgtgaatgc agaattgatt
     3361 ttaaccaatg atagcaattt agctggaaca attgcttata acgaatttag cggatacatt
     3421 cacctattaa aagattctcc ttggatcaat cgtaccgctg gagaatggga agacagtttt
     3481 gaggatgcct taacggcata tattgaagaa aactacaatg tagtttttga tgacaacaaa
     3541 atacacaaag cagtagtaaa tgtggctaga aagaatgttt tcaacccagt aaaagaacgt
     3601 atcgaaaaag ttaaatggga tcaacagcct agactggaaa cactgtttat tgacctttta
     3661 ggggtagacg acaaccttta cacacgagaa gtaacaaaac gttggatagt cggatcagtc
     3721 gcacgtatct ataaaccagg aataaagttt gagatcgttc cagttttgga tggaccgcaa
     3781 gggattggta agagtaccgt tccagcatta ctttacacgg atgatttttt caccgattca
     3841 ttagattcgc taggtgaaaa gaaagatgat tatatgcagc ttcaaggcaa tgtaattatt
     3901 gaacttggag aattatcttc aatgaacaaa actaaaattg aacagataaa aaacttcatc
     3961 agtgccaaga tcgataaaat tagacctcct tatggtcgga atgtcatagc ttgggcaagg
     4021 caatgtgtat ttattggaac gtcgaatgat gggcagtatt tgaaagatga tacaggaaac
     4081 cgccgttttt atcctttgcc ttgtaagaat aagccaaaga tgaatccgtt taaaacaaag
     4141 gatgaatatt ttcttcaagt gttagctgaa gcaaaggtgc tttttgataa aggacaacga
     4201 atctatttta gtcccgatga agataaagaa gtcttagaaa ttgcaaaaga ctatcaagag
     4261 gatgccaaga tggagaatcc aattaaagaa gcaatcacta aatatttgga aatggaaatc
     4321 ccgtataaat gggaagaagc tccagcgtgg gcgagacgtt cttattatca gaactatcct
     4381 gacagtaaat gtaatgagaa aaatttacag catttctata atggagctac tatgcaacaa
     4441 gagttttatt tagttgatag cgtactcacc gctgatattt tggaagctgt ttttgataaa
     4501 caagcaaagg atttattgaa tggtcgatca gatgcagaaa ctaaaaagat tgccttgatt
     4561 attagcagta ttcctggttg ggaacggaag caactgccaa aaagaaataa acgacgagga
     4621 ttttacaata agtcaaatgc ctatgaaaat aaaaatcgag ctggaaagta caaaaagtaa
     4681 cgggtagtaa cgggtcagta acacctagcc cgttactagt ttacccttac tgccccaagg
     4741 gatacagcaa tttggtaacg gggtaacacc ttatttcctt aaaacttttg aaatagttag
     4801 ctaatatgct ggggaataat gttactggat tttacccgtt acctgttacc aaaacgctag
     4861 aacccttgtc cccgttggga ttgagtggta acgggtacct gttacgtagg tgttaccacg
     4921 tgttacgaat caaggaggaa agtaaatgct caatattatt tcaacaaaca aagcaccgaa
     4981 ctttcaatat acagatgaaa tggatcggtt tctaatgaac acgctggcat ttagtgtggg
     5041 attggtaaca gaagattatt ccacatttga tccagaagta ttaaagataa tggaagaaga
     5101 acctgattgg ctgcaggagt cagtagcgtg gtgtcaatcg ctagtagtgg gatcattggt
     5161 agatagtggc aactatgatg atacaggtga gctaatggat gaatttaatt gtctgcttaa
     5221 cctatatgat cgagcgagac aacgagagct tacatcgaat gaggataatt tgtttttaaa
     5281 catccatgat aagttcttgg ctttgctact aacagatgat gaattgataa ctaatttatt
     5341 ggaggtagaa taattatgca tatggaaagt attagatact tcgatggtga gaaacacatt
     5401 gagtattcaa acactcaaga agatgtagac tttattagtt ttgaggaaga taaaatcact
     5461 aaagttaatt ttaaagacgg aacgtgcttg aagatagttt cgccttatat tgaatacaaa
     5521 tcaaaatggg aacaagatga ttctgacgaa cgagatcgct ttggttggta atctatggca
     5581 gttaaaaaac aatgtaatca tgctggatgt aaaacattga ttgattatag gcagaagtat
     5641 tgtgggaagc ataaagcaaa gcaaacggca attaagcgtg aagaaagaaa gcaaagcgaa
     5701 ggtaaatatt ttcaattcta tcaaagtaga acatggcgaa aggcttcata cttgtatcgg
     5761 ttaaatcatc cagtatgtga ggattgccta gaagaaggct tgataaggaa agctgatgtt
     5821 gtagatcaca aaattgaact aaaagatgat tggaatagac ggttagatga aactaatttt
     5881 cgttctttat gccatgcaca ccataattcc aagacagcca atgaaagaca aagacgagaa
     5941 aagagtaccc tttgagttaa gggggcatag tgtgaaactt actgacaatc gatgcctact
     6001 cattttggta caaataaccg ttgtgaaaag gcataagggt aattacaaat aacgattaca
     6061 cttgtaatca caagtgaaaa agtatataat gaaagtagga gatttattga aaaatacaat
     6121 tgttattact gataaaacag ggaaacagcg caccattgag ttacctcatt ttggtcaagt
     6181 gaccattcaa atgcagaacg gaaaaatcat ttatatagat aaattagata aagaaaaatt
     6241 ctgatcgaaa aacgaaggaa tgtgagctta attgcttacg ttcctttttc ttttgtctga
     6301 aaggaggaca acatgggaca ccctaaaata ttacacgata caaaaggcaa tatatcaagt
     6361 gaagaaaaag caatccgtgt ggacgctaga gaagaattgt tcaagcagca accattaata
     6421 aatatcacgc cccctgactg gatggcagcg agcgctagaa gtgaatggaa tcgtatcgta
     6481 ccaacattaa aaaaagatta tccattgagt gaagcagatt atggttcatt ggtagcctat
     6541 tgtttagcct ttgctcgaat gaaaacagcc gaagccgaga taagaaaatc aggtacgttt
     6601 atcacatgcg aaaacggagt aaagaaagcc aatccagcgg ttcgggttca atcacaagct
     6661 atgagtgatt tgaaaaaaca agccacctca ctaggtatga ccttagaatc acgatcaaaa
     6721 ctagctttga acaaggttaa aaatgatgaa cccgaagacc cgttcaaaga gttgatgaat
     6781 tcatgaatga ttacattgag aaagtcctat caggtaagct gattgcaccc aagaaaatta
     6841 tccaagcgtg tgagcgtcat ataagcgatt tggagcgttc taagtcagat agcttccctt
     6901 atgtgttcga tgaagaacaa gccaccaaag cgattaaatt cattgagttg ctaccatcta
     6961 cggacggtaa agcaatcaag atgttaggat ttcaaaaatt cattctaggc agtctttatg
     7021 gctggcgtac taaagaaggg aattacaggc gattcaatcg agcgtttacc agtatgagcc
     7081 gtaagaatgg gaaaacgtat atcgcaagtg gcatggctgc caatgcgttg attatggaac
     7141 aagaaccagc agaagcaagg caagtattgt ttgtaagtaa cgctttgaaa caagctaaat
     7201 tgggctatga tatgctgtct aattcactta gaaacgtggt caagtctagt aagtttttaa
     7261 gaccacaact gaaaattatg aactctaaga ttcagcactt gccctctaat tcgttcgcta
     7321 tggcactggc tagtgaaacc agcacgctag atgggtttgc accaacaacc gcaattcttg
     7381 acgagtggca cgaagcaaaa actcgtaaaa cgtacaacgt cattaagtca ggaatgaccc
     7441 aacaaaagaa cggcttattg tgtgttatta gtaccgctgg gcttgattta aacgttccta
     7501 tgtacgagga atacttattg ttagatcgtg tgctaaaagg cgaagaacaa gccgacaggt
     7561 actttatagc gatatgggaa ttggacgatc ccgaagaaat tcacgatcaa gagaaatgga
     7621 tcaaagccaa tccgattttt gaaagtgaag aaatcaaaaa agtaatgatt ccaaccattc
     7681 aagatgatgt gaaccttgct ttgaaacaaa ataaccttaa ttctgtattg gtgaaaaact
     7741 tcaatttatg gagacaagcg agtgaggaca gctatatgat tgctgaagac tggcaggcaa
     7801 ccgaagtaga accacaagat attacaggca agcccgttta tatcggagtg gatttatcta
     7861 aaacagatga tttaactagc gtttcatgga tcgtaccgct agataacggc gaactttatt
     7921 gtgattcaca tagctttgta gccaccaaat atgggcttca agacaaagaa aagcgcgacg
     7981 gtttgcctta tcgagaacta gaaaaagctg gtgagtgttc cattactcaa ttagaaagtg
     8041 gaatcgtgga ctatgaccaa gtatttcagt ttattcaaga tttgattcaa gaaaatgatt
     8101 tggaatgtat ggggatttgt tatgacccgt ataacgctaa ttcgcttatc agtaaagctg
     8161 aaaaagccaa ctacccaatg ttagaagtaa gacaaggaac gattactcta aacgttccga
     8221 cccgaacttt tagagaacaa gtttatgaag gcaacgttat tcacaataaa aatacgattc
     8281 tcacccatgc agtgaacaac gctattttaa aaacggataa caacggcatt cagattaata
     8341 aatcaaagaa cagtaacaaa attgatccaa tagctgcatt aatcaatgcc tatgtgtttg
     8401 caatggatta cttcaccaca acggaaggag cgaaagcaga caatgaattt tatacaagtg
     8461 aagaattttc tttctaatta cattcatacc gttcttttac ttcttggatt ggtgtgtgtg
     8521 ttggttgcta tcaccttact aacaaatgtc tactatggct tgttagcgct gggcatagtg
     8581 cttattgggg tagcggtcat gctaaataca gaacagaagg gaggttaaaa gatggcattt
     8641 tttaaagcga gacaaaatac aacgggagat cctttcctac aacatgtggt atcaatccaa
     8701 tcggatgatt acaccaccag ctttacaagc gttcgtgcat taagaaatag tgatgtgttt
     8761 gcagccgttc gaattattgc tagtgatatt gcttcaagtc cgattcaatt ggtcaaaaac
     8821 aatatgccac aagctgataa tgaactggtg aagttactaa acgagaaacc taattcagaa
     8881 atggatggtt ggcatttaaa gtttgctttg gcggtcaata tgttgctaaa cggtaatagc
     8941 tttgcagaga ttaaacgcaa cggtgaaaag gtagaagaaa ttcacttatt acctaactca
     9001 agtgtaacgg ttactcaatt agataatggt acgttgtctt atcagattgg tgataaaaaa
     9061 agacgtgtga agtctagcga tattttgcac ttcaaatatt tcacccaaga tggtttaaca
     9121 ggattaccac cactttatgc gttacgtgat gaactgaaaa ttcaacaagc tggcaaccgc
     9181 acattgcaca atttctttac tcgtggtgtc agtgggtcag gtattttgaa ggttcataag
     9241 tcagatttag acggatcagc aaagaacgca attcgtgaaa agtttgaaga agccaacggt
     9301 tcaagtagtg gagataatgc tcttagaacg atcattcttg atgaaacaat ggactacaaa
     9361 acattagaaa taaacaccga tgttttgaag cttatcaatt ccaatgattg gaacacaaaa
     9421 caaattgcca aggcgtttgg tgtaccgatt gagcgtttag gcgttgaaaa tgaacattct
     9481 agcaccgttc aaagtaatct ccagtatatc caaagtacgc tgattcatta ctttaatgtg
     9541 tttgtgagtg aattagatac gaaacttgaa accaatatcc gctttaattc cgatcagtta
     9601 ctagagactg atcctgaaac aaaagtaaaa aatgtattgg atcaggtcaa agggtcactt
     9661 ctcacgatta atgaggggcg gtcgaaaatg gggctacccc ccatggatgg tggcgatcgt
     9721 ttactagcaa gtttgaactt tacgtattta gatacgttgg agaaatatca attaaaagaa
     9781 caggaaggag ttacaccagt tgaataatga agaagaaaag gaaaaacggc tgacagaaga
     9841 agctgagcta acagccgatt ctcccaaagt agagaaagaa aatgaaaaac aaccaacaga
     9901 cggcaaaact atttcaggct atgcgttgaa attcgggcaa ccgtcaaaag atttaggcgg
     9961 ctttgtagaa gtgattacac ccgaagcatt aaaagaggtg gatttatcaa atgtgttctt
    10021 attatatgga cacgattatt caaagccact agctagtgtc aaagcaggta cgttgaaatt
    10081 gaatgtcgat gatacaggat tacattttga agcagagcta accgacacga cctattcaaa
    10141 tgatgtgtat gagaatatct caaaaggggt tgttgattct atgtcctttg ggttcgtgct
    10201 tggattagat tcattcgaca aaaaagaaga tggcacaatt gaacgatcaa tagataaaat
    10261 caaagcactt aatgaaatta gtgtcgtgac cgttcccgct tatgattcat caaatgtcca
    10321 agtaaataag cgttcttacg aatcgtttat gagtaacaac caagcaaaac aaacaaacaa
    10381 tagcttagaa tccacttcta aagcacaaaa ggagagtaat aacatggaaa aaactttaat
    10441 cgataacgaa aaaacagaat tgcgtgggta tgaagaatat atccgttcac aaggcgaagt
    10501 gcgtgatgga gtcactactg taaatgcagc ggcagttgtt cccgaagaag taatcggtga
    10561 agtctttgat ttgaaacgtt caaattataa cttagctcaa tatgcaacag taaaaacagt
    10621 atcaaatgga caaggtaaat atccagtagc aactaaccaa caagcagtgt tagcaacaaa
    10681 agctgaactt gctgaaattg gtgatattga cgctgaaatg tttacttcag ttgattataa
    10741 agtagaaact cgtgctggta agattgcctt atcaaatgag gttgtggaag attcagcagt
    10801 gaatattgta caagaggtca aagatcagtt agcgaaattg gtagaaaaca ctgacaataa
    10861 gcatatcatg gatttattaa aaacattcac taagaaaacg gctgctacgt tggacgattt
    10921 gaaacaacta tacaatgtgg cattagaccc agcattaaat aaaatggtaa ttctaaacca
    10981 aagcggatat taccacctag atacattgaa agattcagat ggacgttaca ttttacaacc
    11041 cgatgtgaca gcacctagtg gtaaatcatt attcggtatg ccagtagtat tgattgcaga
    11101 tacattgttt gccaatccta aagctggaac attccctatg attatggggg atattgcaca
    11161 atctatcttt gttgctcgta gaaatcaagt aacgactcaa tgggaaaaat tcgattacta
    11221 ctcacaagga cttgcagtga tcgttcgcaa cgattataag aaaattgatc aaaatgcttc
    11281 agtgtatatt gagtttacgc cagttgtaac accaaaagta tagaaaaata ttgggcggcg
    11341 gtttatccca ctgccttttt ttcattagga gagataaaat ggtaattttg gatagcataa
    11401 aaaaaagtat gcggatcgat cacactattg atgatgactt tattcaacaa ttgattgata
    11461 cagcaggcga atatataaaa agtgctattg atagtagcgc aacggataaa gatatggata
    11521 attatcagca atttgatttg gcggtgtcat tacttactca acattggtat ttgaaccgtc
    11581 aagaagccag cagcgaacgg ataccagtaa cggtacaagc attagtacaa caaatgagag
    11641 gtgcttatta tgccaatcat taagaatgta aatgaattga ctgagagagt caagtttaaa
    11701 aagacaaaac gggtaaaaga tgaagatggg cagatggtgg atagtgaaga aactgtattt
    11761 gaatgttggg ctaatgtgcg ttcacaaatg ctaaaagacg tgcttgctag tgtaggtacc
    11821 attcttgaag gaacgttgac gtttattatt cgatacgatc aagattatga actaacaaac
    11881 gatatgaaag tagcttggaa aaacaagagt tataaaatta tttcaatcaa tgaaggaacg
    11941 gcgtttaaag attatacaac gatcatagct aaacagattt cttagaatga ttacacttgt
    12001 aaacgttgtg ggtatttagt ataattaagg tagtaaatga agggttagct gcctgaattt
    12061 tgcaagactt agctagtcga aatctattgt tggactgaaa attgtaggtg tggttgcaaa
    12121 catgctggac taagaaagat gatagatgat gaacgttcaa ttttaataca ggcatgctag
    12181 gactgattgg atggttttgg caagctgatt gtgtagaaat caaagaacac gtctgttgtg
    12241 gacgtgtttt ttttgttata attcaaaaga ggtgatttaa tggacaaagc agagtttaca
    12301 aatttttatt ggaagtatta tctacattta gaaaatgagt ttattaatac cacagatttt
    12361 gtgatgctcg atgagaataa tttcaaaaca ttctctattg aatatcaaaa actactacta
    12421 gcgattggtt cggagtgtga gattattttc aaagaacttt gcggatttga tagcagtagc
    12481 aataaaagca taactgattt taaagctgtt attaaatcta gcgaattaat gtgtttggat
    12541 aatggtgtta gggttatgaa ttctttaaca ttgacttcac taaaaccttt tggagatggg
    12601 tggcctgaag atacaccaga ttggtggaaa atatataaca gagtaaaaca tggtcgttct
    12661 tcaaattata aggaagcaaa tttggaaaat gtgttgtatg ctttagcaag tctatattta
    12721 ttagaggaat atttgcataa gaaagtaata ctagaaggtg aagtagactt tataagtcca
    12781 gagtcaaatc tatttacgtt atcttgggaa agaaaacatt cgtcgatgca aaaagtgacg
    12841 tttgaaaaag tggattcaaa ttatgtagct ccactcattg attttaaaga acaagaagaa
    12901 atttaaaaac ccccacggct tattgaagtt tttgtatgcc atattgtatg ccatttggtt
    12961 taaatcgctt gaaaaatagt gatagataac tctaattcat ttagtcataa acgctgtttt
    13021 gataaccttt atagttcttt gatatgggtt tctcattcat ggcggaagaa gaagaataga
    13081 acaaaagaat gaattaataa gatataaaaa aagggattgt gacaaacgtc ttgtcacaat
    13141 ccctttttcc aaataaacga tgttcaatca gctgcccctc ggcattcgct cgaaaaacgg
    13201 cgaaatatga agaactattt tgccatttgt ttctctaata atcaggtcaa aacgttgact
    13261 tcacagtctc ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    13321 tcaacaaaca tgagaagctg ttttttgaaa ttgttccttg tgactcggag ctgaacggca
    13381 ggctcacgac ctctttctta aacggtgttc aaaagcttgc atctgcggta ataagttcaa
    13441 ccaaacgaaa attatgagga gctatttttg tttggctgtt cttattactt gatgcaggac
    13501 agctttttca caacctcttt tcgtttagca ctaaatttcc ttaaaaagta gaaaagcttt
    13561 cgaagcctcg tttgtttctt ttactgtata ataaaagaaa agagtaacaa tcgctaaaag
    13621 atataaacaa gagaaaggaa atacaagatg attggtctga tttattttgt ggtgatcgtt
    13681 ctggcgaaca cagttggcgc tatttccggg atgggtggag gcgtgttgat caaaccgatt
    13741 cttgatctta tcggcgccca ttctgttgca gggatttctt tttactcaac agttgcggta
    13801 ttcacgatgt ccattgtttc gactgtgcga caagtatcaa gcgggaaatc actgaattgg
    13861 cagattgtcg gttgggtatc tagtggagct gttgttggag gagtagtcgg caacattgta
    13921 ttcgaagttt ttttacagtt atttgaaaac gagaagcatg tgcagctgat ccaaattttt
    13981 ttgacggtat tgaccttggt ttttgcgttc ttctacacca agcatcatca gccaaaattt
    14041 catttgacgt catggacttg gtaccttata tgtggggggg gttctgggct ttttagctag
    14101 ttttttaggt attggtggtg gaccagtcaa tgtttctttg ttgatgctga tgtttgcatt
    14161 gccaataaaa gaagcaacac tatattctct atctacgatt ttcttttctc aactggcaaa
    14221 attggtaacg attgctttaa caagtagctt tatgcgcttt gatttatcta tgttgtttta
    14281 tgttatcccc gctgctgtta ttggaggttt gtggggagca agatttagca gggttttgtc
    14341 tccaaaaaaa gtgaccttta tttttcaagt aattgtcctc gtggttcttt tggtcaattg
    14401 ttacaatgcc tttgtcatct tacaaacatt aggagaatct agactaacgc aagtcaaaat
    14461 cactgaaaca tattgttcta aatgaaaaaa agagtatact atgataaaaa aattcatttt
    14521 aaaaaatcag aaattggctg gtgatttgga tgttacggga aatgcgttta gaagatgcag
    14581 aagcactacg tgaaatcaat gcggatcaat tagggtatga tataccatta accgttacgg
    14641 tgagacaaat gaagaaactg ctgaaggagc cagagaaaaa ttttttcttg gtttatgaag
    14701 atcctacttc taaaaaagca gctggctatg tacaagcgga gtcctatgaa tcgattttta
    14761 gcgatccaat gttcaatatt atggcacttg ctgtgtctaa atatgctgaa aaaagaggca
    14821 tcggaaaggc tttgatgact ggcgtagaag aagaagcgaa aaaacgagga ataacgacaa
    14881 taaggcttaa ttctgcagaa tacagagtag aagcacacca attttatgaa cacatcggtt
    14941 atcattccga taaaatgcaa aaaagatttt tgaaaatcct gtaaatcttt atcaaaaaaa
    15001 tacccgatca ccaagccgat acttgatgat caggtatttt tgtttttttg actaagaaag
    15061 taattgcatc aagaaaccag agatgatcaa aacgatggca ccaccaagtc gattgcccat
    15121 ttgagcaaaa gcaataagtt ccatccgatt agctgcagat aagacggcta catttcctgt
    15181 tccgcccatc gagttgttgc ataagccagc agtaatagtt gattcaattg gatataaacc
    15241 aaacaaacga ccgacaaatc ctgaaacaac tgaaatcgcg ataacacttg tcagacataa
    15301 gacaacaaat tgccatgtca aagcagaagc taatacattc agattcagca acgcaatacc
    15361 gataccggca agaacagctg ctgtcaagtt tttgacaatc aagttattga acatgatagc
    15421 tgcatcttca taatactctg gtaataggtt ggcgatttta cagatgacaa cgataataat
    15481 cataaatgcg taagcatgga ctttaggaac aaaatagtta ccaatttgtc ctaagatgaa
    15541 gaaagtcaat gataccatca aaccaacacc taactgtgga atggaaggtt tcacagttcg
    15601 aggttttcca tcgccaagat cgcctttttc cattaattta ccattgccgt tgaattttgg
    15661 cattgattgt ccagcacgag caactaatgc tgcaccgatg atagctaaga catttgtcat
    15721 tgctgtcgca ggaatcaggc gggaaatgat agaagaagca tccgtaccta aaacatttgc
    15781 ataaatcgtt gataaaggaa cggctccggc tccaacacca ccagccatcg caggtaatga
    15841 gatgtataaa acggaatttg caaaaccatt tccgataagc atgcccacga gtcctacggc
    15901 aaaaaaggag acgaccattg aaataagtgc aacgggaata aaacgaacag aagctttcat
    15961 caatagatta cggttcatac caaggatgct accagtaatc aaggcagcaa tataaaagtc
    16021 ccaaaagccc atattgctga caaagttttc tgtggatttc actacatatt caggtaagat
    16081 cccaaatgta gcaatcgcag cagaagcgaa gatcgcaaaa acggaaccac cacccaaata
    16141 acttttgacg atagggattt ttgtcccgat aaaatgaaat aaattaccga aaataaccaa
    16201 aactgcgatt ggtccaatca tattcgtcgg taatttccct aaagccattg caatgatcag
    16261 gacaaggacc atgatcaaat aaagtggcaa acttacgccg gtgatttttg tggaccaaaa
    16321 accttctttt ttcttttcct caactgcagt tgcagttgtt gttttttgtg ccatttttac
    16381 agatcctttc ttattctgta tcatatgaaa aaagccaaag aaaagcgaca aaagagcagg
    16441 agttggctgg ccgctgtttt gccgcagtcg cttttttctt tttagacgtc atacaaagtt
    16501 tctgtatgtt cttttactat aacgatctta atcttctaaa gatttatatt caggaaccca
    16561 tttagcagat tcaactgctt ctttgatatc cgtgatttct tcacgattca agttttgtgc
    16621 gacaactgat tttgcgacag tttcagcaat cgtttgagaa aattcagtga tttttgctac
    16681 tggaggcaag atagctgctc ctggttttgt tacatcaacg attccgccta atgctcgact
    16741 agcttgagaa atgatttcag catttactcg tgtagctgtt gaagcaatca aaccaagtcc
    16801 taaacctggg tacatcaaag cattatttgc ttgaccgatt tggtaagtta caccattata
    16861 ttcaacatca gcagcaggga tacctgttcc aactaatgct tttccgtccg tccattctat
    16921 taaatctttt gcttttgctt ctgccaattt tgttggatta gacaaaggca tgatgatcgg
    16981 acgtggtgta tgagcagcca tttctttcac gatttcttca gtgaatgctc caggttgggt
    17041 agaagtacca atcatgattg tcggatggat cgcttttaca actgcttcta agtttgtcaa
    17101 ttcatcagca ttagtgaaat ctgagcgttt gcgagcgaat gggatttgac caggagtcaa
    17161 tccttctgta tcttcaaaca ataaaccttg tttatctact tgatagaaat gttggcgtgc
    17221 ttcttcttca ggtacacctt gacggatcat ctcatctaaa aggatgtttg cgatacccac
    17281 accagcagta ccagcaccaa atgtcaaaat tatttgatct ttcaattgtt caccggatat
    17341 attcaatccg cctaatacac ctgctaggac aacaattcct gtaccttgga tatcgtcgtt
    17401 gaaagtagtg atttgatctt cgtatttttc taagatcgct gcagcatttc ctcgtccaaa
    17461 atcttcccaa tgcaataaca attctgggaa taattcggta gctgattgaa cgaattgatc
    17521 gatgaattca tagtaagttt cgccttctac acgagcatga cgattgccta agtataaggg
    17581 attattcaat aattcttcat tattagtacc agcgtcgatc gatacaggca aaacttgtgc
    17641 tggattaata ccagcagcag cagtatagac catcaatttg ccgatagcga tgtcaacacc
    17701 gttcacaccc cagtcgccca tacctaagat tccttctgca tcagtcacaa caatcaaacg
    17761 aatgtctcgt ccatctgccg cgttcttcaa acttgctttg atgttttctg gttcgtcgat
    17821 tgaaagaaat gcagcatctt gcggtttcaa gaaaatttcg ttgtattgtt cgattgaatc
    17881 cgcaacgaca ggatcataaa cgacaggcat gaattcaact aaatgttcgc ccatcaattt
    17941 ataaaacaat gtccgattcg tattgaatag attcattaag aagatacgtt tttccaaatc
    18001 tgtttgtttg cttaagtatt gaccataagc ttgaacagat tgttgttcca gtgtttggac
    18061 agtacttgga agcataccag tgattccata ttttgcccgt tcttcttttg taaaagctgt
    18121 tcctttattt agaaaaggat tatttaataa gtttaaccct gtaagcatga atgattcctc
    18181 ctaagtttta atacaaacga agtatagaag tcagatgatg atatagtcaa aaagagcagg
    18241 tatgataaat tttatttata tgtaatatag ccaaaagaaa tcaaaatagc agtatttgtt
    18301 ctttttatat cgacaatcct tgcttgaata ctatgcgtat ctcttatatg ctagagaaag
    18361 aaaagtagaa gaataaggga tagatggaat ggataaatag gatttctgag gaaaaaagca
    18421 catgatagcg ttgataggag tcacctatgt caggtgataa aaatacataa gtagaaaatt
    18481 aagaggagga agagaaaatg aaagaagttc aattgattag aaaaagcgaa ctatcagaag
    18541 gcggttgcaa tgcatgtggc gtagtagaag cgacaagcta tacattaaaa ttagattcca
    18601 atcaagcaat aatctctgaa ctgacagtag gcggtcttgt ggacagtctg gcgcttgctg
    18661 aaggttttac tggagaagat atttatgaga tgttttcaga agtccgtcag ttaaaaaaag
    18721 gagaaaaatg catagaggtt catcatgaga gcccgaatgt gaggttcaaa cgtggtgata
    18781 atgagatgat cttcaagaat catgtgtctg atcacactga actatatgaa atcgtgaatc
    18841 aaatactgtc tgggattttt gaattaggac cttatgagtt caaggaagaa aacggtaatc
    18901 caaaattaaa tgaagaatgg caagaaacga ttgaaacaca aagaaacaat ccacacttat
    18961 ttcaatagtc ttccacagta tttcgtaaaa atgttgatga aaaagaaaca ccgtgaatca
    19021 gaggtcgtga aaaacctgcc ctgcatcaag taataagaac agccaaacaa aaatagctcc
    19081 tcctattttt cgtttggttg ggcttattac cgcagatgca agtttttgaa caccgtttaa
    19141 ggaagagact gtgaagtcaa cgttctgacc tgagtattag agagacaaat ggcaaaatag
    19201 ttcctcatat tttgccattt tttgagctaa taccgagggt cagctgattg aacaccgttt
    19261 tatcgagagg ttgtgaaaaa gtcgcttagt ttcgagtagt aagaaaaatt ttgtgaaaat
    19321 ggctttccac atttttgcat aaatttgact taataccgag aaaatggctt ttgaacaccg
    19381 tttattcaaa aaaagggact ttgacactac ttttgtcaaa gtcccatcgg ttttttatct
    19441 atttttatta caatcatttc ttccagagta gtgacatctg gaatggctga aatgtaaaca
    19501 gattatttgc ctgtatcaat gtcgaagagt aatataaaaa gtgtaaacgc tatcatattg
    19561 ttcatcattc aaattttata gagagaaggc attgagatgg aaaaaacata tcaagcaggt
    19621 acaaatgaag gaatagtaga ttttatcaat atggaagatt tagaaatcgc ggcttcacaa
    19681 gtgattccag ctggtggtta tggttacatc agcagtggtg ctggcgactt atttacttat
    19741 caagaaaatg aacgggcatt caatcatcgt ttgattattc ctcatgtatt aagagatgta
    19801 gaattacccg atacaactac acattttgac gaagaaatgc tgactgcacc aatcatcatg
    19861 gcaccagttg ctgctcatgg attagctcat gtgaaggcgg agaaagcttc tgcaaaaggt
    19921 gtagcagatt ttgggacgat ctatacagca agttcttacg cttcttgtac tttagaagag
    19981 ataagagaag caggcgggga gaaagcaccg caatggtttc aattttatat gagcaaagat
    20041 aatggaatca atttggatat tcttgaagtg gccaagcgta atggagcaaa agcaatcgta
    20101 ttgacagcgg atgcaacagt tggcggaaac cgtgaaacag accgcagaaa cgggtttacc
    20161 ttcccgctgc caatgccgat cgttcaggct tatcaatcag gagtaggcca aacgatggat
    20221 gctgtatata aatcttccaa acaaaaactt agtccaaaag atgtagaatt tatcgctgct
    20281 cattctgatt tgccagtcta cgtgaaaggt gttcaatcag aagaagatgt ctatcgatca
    20341 ttagaatctg gtgctggtgg aatttgggta tctaaccatg gtggccgtca attagatggg
    20401 gggcctgctg cgtttgattc gttgcaatat gtggcagaag cagtagataa acgtgtacca
    20461 atcgtgtttg acagcggtgt acgacgtgga caacatgttt tcaaagcaat cgcatcaggt
    20521 gccgatcttg ttgctattgg cagacccgtt atttatggat tgtctttagg aggaagtaca
    20581 ggagtccatc aagtgtttga tttctttaaa acagaattgg aaatggtcat gcaattagct
    20641 ggaacacaaa cagtagaaga tatcaaaaaa ataaaattgc gtgagaatcg ttttatttaa
    20701 gaaataaatg acgaaaaaaa taggagtgcg gcagatgatg ccgtactcct atttttatag
    20761 ttcaaggatg tcactaatag aataatttga aacgaaaaaa taaaattgaa atattttttc
    20821 atacttaata aaaaaggaag aatattcatg taaacgattt tattgttttt ttctctttta
    20881 tacaaaaagg gtcataattt caagtaagcg gatgaattca tgaggatttt ttgcatactg
    20941 tctctactat acttaggtca ctaagaaaaa ggagtgattt attatgaaaa agaacatgat
    21001 tgggacatta ttactttcaa cacttcttct aggcggcttg gcaacaccag cttttgctga
    21061 gggtcaagcg acttcaaaag gagatattac ctttactgaa ccaacaaata ccgttgagcc
    21121 tcttaaccct actgatcctt caaaaccagt agagccagca gatccggaaa atccagcaac
    21181 tggccagaca ggttctttaa cattggatgt agttcctgaa ttgccatttg gtacacacga
    21241 gattgaaagc ggaacaaaaa cgtatcaagt agacgcttca aaaaacgata caccttatct
    21301 ccaagtatca gatcgtcgag gtgtaggtgc agatggccaa gcacaagggt ggaacgtgac
    21361 agtttcggtt tcagattttg taaacggttc acaagttttg caaggcgctg aattagattt
    21421 tggaacatcc actgttaaaa gtacgtcaga taacgaatcc actggaccaa ctagtcaaac
    21481 tgtaactggg ttgagtaaag caagtgcagc gacaccaata tttacagcag caaaagatca
    21541 aggtctggga acatggcttt ctgtttatga tccagcaaat atcacactga aagtgccgaa
    21601 agcagcagct ggaacattca cggctgattt gacttggaac ctcgtggctg gcccagtagc
    21661 atagagtatg aaataaattc aacaaactgc ggtgaaaata ctataggtga aggatttttt
    21721 accgcagttg ttgttgaaag gatgtggtgg acaatgaatc aaagtaaacg actgtttgtt
    21781 tcgttttttt ctattttaag tctatttttc atttttccgt caatcagcca agcaaaggat
    21841 tcagcaggag actttggaat caaaccggta tttccagaga accaaataga taaagcaatc
    21901 ggttattttg atttattagt ggcaccggaa caaaatcaga cactagaagt aattatcagc
    21961 aattcttcag atgaagaacg aacatttgag gtctctgtca atccggcggt aacgagtgat
    22021 ggtggaacga tcgattatag tcaaaaaaac ccaacgttag atgagacatt gccgtttgat
    22081 gtccgtgacg ttttgctcat tgcgaaaaag gaaatcaatg tatcagcaca tgcagaaaca
    22141 actataccta tagaagtgaa aatcccagca aaaagcttta agggacgtgt attagcgggt
    22201 attcatgttt cacctaaaga agaagctgaa acagaaaatg ctacagaagg agcacaaatc
    22261 aaaaatcgta ttgcttataa tctcgcagtc gttttacaag aaagtcaaga gacgatagaa
    22321 ccggatctga aacttttatc aggagatttg gacgaggtca acgcaaagcc aactgttcag
    22381 cttcgtttcc aaaatcctca gcctaggatt atcagcaatt tgatatttac gagcaagatc
    22441 ttttatgaaa atcagcttta tattgaaaat acgtctaatg aatttttagt tgcaccaaat
    22501 tcgaattttc acttgaatct tgatttagct ggtgataaag caaaagccgg cgattaccgc
    22561 gcagaaatca tagcaaaatc aggcgacagt aatgaatggc gatttacaca aaactttacg
    22621 atcaaaaaag aaaaagcaca aaaagtaaat gaaaattccg tttttgcagt acaagaacaa
    22681 tctttcccat ggctttatgc aggattggtt gctgcagttc tagtgttatg gttgattttg
    22741 ctgtttttgt gggtccacaa gaaaaagaaa aaaggagaaa caacatgatg aggtgatttc
    22801 gttgaaaaca tcgatcttat tgggagggat tattgcaagt ttgctgttct tcagctggca
    22861 agagccagtc caagcagata ccaaaagttc aggtacgatt acattttatg atctaagtcc
    22921 agttccgcat ccaagtggtt cagtcaatgg cgcatcaaaa cagcagacac agctaccgat
    22981 ttataaagaa tctaatcaac atggcgtatt cccggagacg aatgaagaaa agatccgttt
    23041 tctttatttg atcattggta tagaattatg gcttcttgcg tggttgttgt tatggagaag
    23101 aaaggaaaag gaggatgaac atgttgctac aaaataaatt ttcatttttt cgctccttct
    23161 tttttacttc gctttttgtt ttaagtatat ttatcttttc accttcggca gaagctgttg
    23221 gagcaacgcc tcctgccatt tcttatgatc catacacgta tgaaaatatc gtacctccaa
    23281 atggtggacg tagtgcagtc aatggcggga agcttgtcca actaactggg gctaaccaat
    23341 tgcctaaatt gactgctact tatttgaacg atggttcaaa tgatccttgg aaaggtggca
    23401 cggtccgttt tcattgggaa agccatgtga aactaacaaa tgtacttgat ttggtgaata
    23461 tcactgcaca aggatttgca tttgtggcga tcatcaattt gccggaacat gtatcaagca
    23521 gtgatgtatt aaaagcaatt gactggaaca acgcctactt gtatattgcc ggtgccccca
    23581 tcaaggttac tagcggtagt ttattatcag caggaagcca taccttgcgt tttgggatgg
    23641 ggtcgtggga tacaaatact ctattggcag atattattgc tattattacg cgaggcgaat
    23701 tcaacgtgga cgatatcccg cttcaatttg atgttacagc taatgtcgcg aagatgacag
    23761 aaaacggaga taaatatgat acgagtccaa acaaaatttt gaccaagggt aaattacaac
    23821 cttctatgtc taaaaaggcc gttttttctg ttgattttta tgattcgaat aatattatcc
    23881 aagatcgtat gttcaattcg attttagctc cagctgcagg tagattttat cctaaattaa
    23941 ctggcggaaa cttgaatcca ggagcatatg cattttctgc aagtgcagag ataaatacgt
    24001 ggaatagtta catctcaaca agtgattcaa caggaaaata taatacattt gctgatacca
    24061 cagaatacgt ggatggaaat aaccagaacc ttccaggctc taccaatagt cggacattga
    24121 cgatggggct gactgaagga cgttttgatc agttgccatt tagtcgcttt gatcgcgtag
    24181 tcaactattt tacaggaaaa aacgttacac aaggagcaat actgactcat actccagcaa
    24241 gcacaagtgg attgaatcag acagccaata tcgtgtacag tgggaaagat gcgcaaggcg
    24301 gattgctttc tcctgttgct ttaaaggtat tggagcagta caagctagac ggaacggttt
    24361 taccaacaaa cttaacagcc gatagtcagt ggggaaagct cctttcttct gcatcgtacc
    24421 aagtcacggc caattggtca gcaactgaac taaaaaaggg ttcgatccat tacaggcttt
    24481 attcgaaagg aacccaaaaa ttagcgacag gatggacaga tcaacttttc caaacgatcc
    24541 aatcaaataa cgggggtgta aatacagcta cttctacact acctgcattg gcttcagggc
    24601 aatattattt tgattatcgc ttggtggacg atgtacttac taacatgtat ccgtcttttg
    24661 cgtacaaatg gcagtctgaa caagctggaa tcaccactct tcctcaaata acagtggcta
    24721 attttccatc tgtgagtgca gatagtcagc taaaaaacct tagcagacaa cctgagacag
    24781 gagatcccct gtctgctcta tcaggagata cgattcaaga aaatgtgaca ttcacgttaa
    24841 caaaatctgg ggatgcgata accgacaaaa aaatcacgat ctcacttcct gaaaatacta
    24901 cttatatcaa tggttcctta aaattaaatg gtacaactat cgcagatacc ggcattcaac
    24961 aaggagtttc tgtcccagca gatctattga gcaaaatagg agataccatt catcttactt
    25021 acaactatca attaaatact gtagatactt ctgtacaatc cgtcagcata ctgacaaagg
    25081 cagctgtact ttccagcaat attacattag cagatggtgc taagcttcct aacccagttg
    25141 tccaaacatc tgctaaaaca attcttgtcc ccaaacaaga attaacatta gttaatgtac
    25201 cagatgattt tacatttggt aacgatctcc ccaaaccgtt gaagacaagt tattatgaag
    25261 caaaaggcga tttttctttt gatgtgcggg atactcggct tccaagcacg tctccttggc
    25321 aactgacagg gacattgact tctttattta agaatgatca aggtcaagaa ttaagcggaa
    25381 caaaattata cttcaatcat tctggatcaa aacaattgat tcagcaaggt caaaacacgt
    25441 tgatttatga gagtgatgga acagctaaag gagaggttct cgttgacttt cccgataccg
    25501 atggtttact cttagaggta aatagcagta cgaatgctca acctggcgca acgtatcaag
    25561 gcatggtcac atgggaactg acagctgggc caaccagcta agtgattaga atttgtatgg
    25621 atctgtgata actgtcacag atcctctttt tcaaggatga gaaggaaact tctgctgtta
    25681 aaatcagaaa tgcttggagg agtggacttg gaacttttca gtttgcttga aaaaaatgaa
    25741 caacggcaaa tcaaactttt ccaactttta ttagaggaaa aaggagagat ttccgttaat
    25801 aacgtactta cccgcttatc agttgatcgg gctaccttga aagaagatct gttttattta
    25861 caagctaact tggaaaatat caaacaattg gaaattgtta tccaagaagg gaatatttta
    25921 ttaatcagac atggaaatat agcagcagtc gatgtttatt atcattgcta cttgcgctat
    25981 tctacaaaat atcagatact tctttatctg ttggaaaatg gctcatttga tcggcagaaa
    26041 cttgtcatgg aacttaatat cagttcggct actttgtctc gcagagtgaa ggaattaaat
    26101 gattctttaa atgagtttca tctgaaaata aaaagcgggc ggctgaccgg tccagaaagt
    26161 caaatttgtt atttttattt ccaattattt tggtttggac gtccttatct tgaaaacaaa
    26221 aaagaataca gtgatttatc aggaaacact tttttagatc tgctgcaaaa agagtttagt
    26281 tttactatta cagaagaagg acgattgaaa tttgaaatat ggctttccat tatgaagaag
    26341 cgaatgcgct gtacgaaaaa atccaaacca gattatagca atcttcctgt tggattgttt
    26401 caaacgacac aatttttatt ggacctccat acatttttga cccgttactt ctttcaatat
    26461 gcctttcctt ggaatcaaaa tgaaacgttg gttttttatc ttttcatgat cagtaacttt
    26521 acattagata cacataactc gcatgtccag gagtttttga taagcgaaaa atacaaagac
    26581 gacgtagtag ctcaattgaa tcgaacgttt aaacaatcga tagaagaata tttctctttt
    26641 tatgtttttc cagaagagat ttcgcaaaag attgcgttga cagtgatgca aaatcacgct
    26701 cattttgcct attttagagg atggatgaat atttttggcc aagaaggatt aaaacagcgg
    26761 ttaactgaag tagaagataa acaattcctg gttttatgtg aaagattggt cagacaaaca
    26821 tttgctattg ttgattttac tgaagtagac gaggaaaatg cccgctttga attatttgct
    26881 cgctatgcat ctattttgcg gacgctgatc aagcagttag aattacgtct gcaaattgct
    26941 tatgatcttc cttatgaaag gctaatggcg gatatcgtga aagaaatcat ccaggaacaa
    27001 ttgctgaata cgatcaaata tgatttagtc gaatacgaga aagaaaagca gtatgatatc
    27061 attttgacta gccaattgaa agagtacccg tcacagaagt ccgctaaagt ttttgtattc
    27121 actagcaacg aatataatta tgatttcccc tatttgaacc aatttttaaa agaatgttat
    27181 ttagaaaaat tgaatttaag gatgaataaa acatgaaaat cgtgatcatt ggtgcttccc
    27241 atgcaggcat cacagctgcc ctaaatctaa gaaaacttca accgaaagca gaagttttac
    27301 tgatcgatga agatcataaa gatggactag gctatgtttc aaatggaatc aacctttatt
    27361 taaaaggaaa gatacgtagt ttagctgaag ttgcccataa catgcggact cttcaagaaa
    27421 gtggggcaaa actgatcacc gaatggagag taactgaact tgatccagac aaacatcagc
    27481 tcatcctcgc atccaaagaa ggaaaaaacg agaccataac ctatgataaa ttaatcgtag
    27541 caacaggttc ctctcctgtc accttgtata aacaaattga agctgaaaat gtctacacct
    27601 ataaaaatct agtgcaatct aagcaagtac ttgccgcttt aaaagaagca aaagaagtag
    27661 tgatcttcgg tgctggttat attggattgg aattagcaga tgctttgaga aataaaggat
    27721 acattattca tctagttgat tatatgccga atgttttatc aagatatttc gataaggata
    27781 tgatcaacag ttttcaaaat cagctgcaaa ctaagcaaat caatttttat ccaaatgaat
    27841 tcttgatcga ttggaaaaaa tcagaagaga aagtagtatc cgtacaactt ctatctcaag
    27901 caataaaggc agatatggtc attttttctg cccaaactcg tcctaatacg acattgttaa
    27961 aagaaaaagt ggctttgtat gaggatgaga ctgtgatagt caatgaatat ctgcagacaa
    28021 gtgatccgga catctatgcg attggagata tcgtcccagt cagttttgac aaaaataaac
    28081 ggcatctctt tttaccttta gttactcgag ctgtacatat ggcaagagca gtagcgctaa
    28141 ctttgagtgg tcagccaacc gcttacgacc tcagacaaaa gataacagca gcagtcatta
    28201 tagactactt tttaggaact gtagggctga cagaagatga agcgccattc ttggaacaaa
    28261 ataccggttc gtgttcagga gaatttgatc tatttccaca atacgatgaa gaaaacaaaa
    28321 cagtaaatgc aaagctgatc taccatccgg atacattaga aataattggc ggacagctga
    28381 taagccaaga attcttatta tcagatttga acctgcttgc ggatatcgtg aaacacaaaa
    28441 ctacgattcc acagctagca gttgaggatt ttggatttct gtctgaacac acctctcgtt
    28501 ttcactacct caatgagtta gcttttaaag tactagctaa gagagcaaat agcaatagag
    28561 tagataaaag actgtgagaa atatcacggt cttttattgt gtggggatat aataaatatt
    28621 aatcaatcga gaataaaaaa ttcatataat gaaatagcgg aagaattttt tgagcataat
    28681 attcgccaaa aatttatcaa gtgataaaat tgttgtataa aatgattatt tcaggaggag
    28741 gagttaaatg tttttagcat taaatgaaat tatgcattca aagttacgtt atgcgttagt
    28801 tgcaggggtg atgtttttga tcgcctacct ggtatttttt ttgactgggt tagcttatgg
    28861 cttagctcaa gataatcgaa cagcagtcga caagtgggag gcagattcga ttgtgctttc
    28921 gaaagatgca aattcaaatc tagggatgtc catgattact aagaagatag cagaagaagt
    28981 cgaaggtggg aaagtggcgt atcttgccca gacacctgga gtcgtgacta gtaaagacag
    29041 tacagaagaa ggaaaaatca atgttagctt cttcggtatc gataaaaatc aattcatcat
    29101 gcccaatctt gttgaaggaa aagcttttga taacgatgat gaggcagtag gcgatattag
    29161 tttgaaagaa gaatatggtc tagccgtagg agatacagtt aagttatctg gtagtgacaa
    29221 gacgtttaaa ctaactggct ttacggatca tgccaaattc aatgtctcac cagttctcta
    29281 tacaaccatc aatgcttacc aagagatccg cttcgaaaaa gaagatacaa gtgaaaatgc
    29341 acggattaac gcaatagttg tcagaggtaa aatcagtaat ttacctgaag atttagaaca
    29401 gatcaaaatt tctaaattca tcaatgaatt acctggatat aatgcgcaag tattgacttt
    29461 tggatttatg atcggatttt tgatcgttat tgcggctatt gtgatcggga ttttcattta
    29521 tgtactgacg atgcaaaaga ttaatatctt tggtgtaatg aaagcacaag ggatcacagg
    29581 aggatttatt gcgcgttctg tagtggcaca aacattcatt ttatcatttg ttggtattct
    29641 tcttggtcta ttaggaacag tgggaacatc ccttgttcta cctgatgcgg ttccattcca
    29701 aagcaattgg ctgttttttg gcgtcattag cttgttgatg ctggtagtag ctgtgttagg
    29761 cgcactattt tctgttcgga caatcgtaaa aatcgatccg ttgaaggcaa ttggataaga
    29821 aggaggaaga gcatgaaagc aatagaattt aaagaagtag aaaaaagttt ccaagatggc
    29881 gaccaagtag ttaaagcatt gaaagaaaca accatgagta tcgataaagg ggaatttgtt
    29941 gcaatcatcg gtccttctgg ttcaggtaaa agtacttttt tgactttggc aggcggattg
    30001 cagacgccta gcagtggctc tgtatttatc aatgatcaag aattcagtaa tcaaaaagaa
    30061 agtgaacgcg ttaaactacg ttttaaagaa attggcttta ttttgcaagc ttccaatctg
    30121 gttccttttc tgacagtaaa aaagcagtta ttattagtag ataagatcaa acatgaacat
    30181 cgtcaaaaag aagcaaaaga attatttgag cagcttggcg tggataagtt gatcaacaag
    30241 tatccagaag aactatctgg tggagaacgg caaagggtcg caatcgctcg tgcattgtac
    30301 aatgatccgt ccattattct agcagatgaa ccaactgcta gtttggattc agaaaaggca
    30361 cgagaagtag tcgatatctt ggcaaaagaa tcaaaagaca aaaataaagc aacgatcatg
    30421 gttactcacg atacacggct gattgacaaa tgcgatagaa tatttatgat ggaagatggt
    30481 gtattgaaag aaaaagaata accaaaaagc aggtggaaaa atggtccatc gatacaacaa
    30541 agatacacag caaaaaattt tgaaagaagc gactgacttg tttatgtcca agggatatct
    30601 cggcacttca acaagagaga tcgcacaaaa agttgggatc acccaaccca atctctatca
    30661 ttatttcggt gataaagaaa aactatatac ggcagtttta gaaaaccact taaaagatgt
    30721 tggaaaagct ttgagagaaa tcgttcaaac aagcgaaacg ggatttcaag taaccttgac
    30781 aaaaatggcg cagtttttga ttgagacaca tctggtcgat ttgtttctga tgcttcacga
    30841 tcttgaaagc aatctttcaa aggaaacaag agatcatctt ttctttctat ggaagaaaaa
    30901 ttatcgtgaa ccttttgaag aaatcttttc agcaaaccaa tctgtgctaa gaaatgggat
    30961 ttcaaaagaa atcgcagcaa ggcatttttt cttagtgtta gcaccttaca tcactcggtc
    31021 gaaaagacct gtggaagaac cgctaaatgt ggcgcaattg atcgatttat atttgaatgg
    31081 tgtattagga gaagcgtaaa gctgttatat agggagaaaa atagttaggt gaagaagcaa
    31141 gatatgacga agatcgttat atcttgtttt ttttatatag gtagtttttc ataaatacaa
    31201 atttgttatt ctctcatgat tatcagggta tatttttatt tattatgtcg gtgttccagg
    31261 aagtgtttta atttaataac gaagaaatta attgtttgta gaatgaagga aactatttat
    31321 tgggtagatg aatcaccacg aatagtcaaa aggagatttc aggatatgcc aaaaatgaaa
    31381 aaaatcattt tagcagtaat gttattttct acggttatat cttttacagc cccttttttg
    31441 cgtacttaag ctgattttaa gttttgaccc ttagtctgga tctacgaggt gagagtcaag
    31501 aatgaaaaaa ataattggag cttttgtatt tgcaatcatt atcattatta tctgttttca
    31561 aatagatgtt tttggtcaaa aaaagatagt tggacaaacg gtcagcagta cgaacagtac
    31621 ggttacaaag acaaaggaat catttgatga tacagaacaa gagacattaa tcaaaatcgg
    31681 aaaaaatgac cgtacactat atacagatga ctcgctaact accccacttg caactataaa
    31741 cggcggagag ctggcggagt ttctttctga aacaaaaaat gcttatcaga tcaagacaaa
    31801 tgatggctat actggatatt tagccctcga agatggaacc aaagtcacaa agaatataca
    31861 aacaaaaccg gaagagctat ctgatgcagt gattgtatta gatcctgggc atggcggaaa
    31921 tgatactggg gcattaagca atgatgaaca aacagaagaa aaagatataa cattaagtac
    31981 agccataaaa gtaaaaaagg cgctagagga tgctggagca acggtttatt tgacccatac
    32041 tacagatgaa ttagtacagc ttggagatat atgcgactac agtgaagaaa aaaaggcaga
    32101 tgtctttatc agtttacacg cagactctac ggaatatgcg aatgaagcaa ctggaatcac
    32161 gacatattat tactatggac aagaggaaac attagcacag acaattgctg actccttcac
    32221 tgatctgcct ctggattcaa gaggaatctc cactgggaac tatcaagttc ttcgagaaaa
    32281 tcttcaacct tctattttaa tcgagatggg ttatatgaat aatgatagtg atttaaagga
    32341 attagtaaca gacagttatc agcagcagat tgctgcaagt ttgacacaag cattgaccac
    32401 ttattttcag taataaataa gcccagccaa acaaaaatag ctcctcatat ttttcgtttg
    32461 gccgggctta ttatcgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgagc
    32521 ctgccgttta actccgcgtc acaaggagca atttcaaaaa acagcttctc atgtttgatg
    32581 actattgtga cttgtgaccg aggagttggc atgagaacac cgtttattcg gaacaaggga
    32641 ctgtgacatg acttttgtca cagttccatt atttttttag cagcttcaaa gctttttctg
    32701 tattggcaaa atgaagtttg gcatattttt caagcatagg catgccgcct ttctccaata
    32761 gttttgccgc tacttggtca gatttcgttc cagctcctga tggaagggtg attccagctt
    32821 cagctgatgc tttgtgtaac tcttccaaaa agctggcacg actgatgata gaagcagcag
    32881 caacagctaa atgatattgt tctcctttag taacgaagta cagtttttct tgtaattgat
    32941 ttttttctaa acgcgcatat tttttaaaat ttgcttccgg cgtaaactga tcgatcaaaa
    33001 tcgcttctgg tttttctggc tcgatttttt ttagcagcaa atggatcgct tgattgtgca
    33061 aagcgacttt catccgaacg gcattgtatt ttggttggat ctcattgtat ttttctggag
    33121 ataagacaag cagtttatag gggatggttt tttttaagat gtcagcaagc tggatgattt
    33181 ggctatcttt taactctttt gaatctctaa caccaagttt acgtaattct gcaagctgat
    33241 cacgtttcac ataagcagca catactgtta gcggaccaaa atagctgcca tttccgacct
    33301 catcactacc taaaacggat aattgactga tattttttgg caaagaagag gctgtttttg
    33361 ctggcttggc ttgtttatcc tgtgcctccc cccatttatc agcttctatt gctgcattcg
    33421 taccttgaaa catcactttt ccggatgtat aagcggtaat ggtcgtcccg tctttttttg
    33481 cagcaaagct agtataagga atctgtttat ttaacaaata agagcgatag tatgctttca
    33541 tttgactaag ttggtcgttt gttgctttga taatgacgtt gctcataaaa ttctcctcgt
    33601 ttttatacat aaaactatac cgcaactcac gacttttcgt tttctaagag attgcgagac
    33661 atagcatttt tttaaagttt atcatataat agttaagtat gtgaaaaata tttgtggaga
    33721 acggtgaaag aaccaacttc atggtacact aaataaaact ttcatcaaat aggaggaaat
    33781 gattcgtatg gcgcacgaaa aaacacgcta caaagcagtg attgccaacc aaacatatac
    33841 tattatcgga cgggaaacca agcatcatat ggatatcgta acgaagctga tcaatgaaca
    33901 gttagcagaa ttgaaacaat tatcaccaca aatggataat gaacaagcag caatcttgat
    33961 ggctgtcaat gcgctttcgg atcaattgaa gaaacaagaa agaatcttgg agctggaaga
    34021 agaaacagca gaattaaaga aaaaaatgat caaatttaca gagcttgaaa atcgtgtgaa
    34081 acgcattgaa gcaatcgaga acgaagctcg ggaagtattg aaagaaaatg gtcaagcaga
    34141 tcataccatt aaaaatcatg tggaagcaca acagatttta aacgagaaac gaaaaggaca
    34201 aattaaacaa aaatcttcta attaagatcc ttttatcaaa ataagaaaaa tttaaaaaag
    34261 ttagaagaga aaggaacgta gcatgctgag tttgctgatt ttatttttat tgctaattgc
    34321 gtttttttct ggagcacgtc gcgggtttgc tttgcaaatc atattcgcga ttggatatgt
    34381 attgtcattt attgctgccc agcattttta caaaccatta gcaagtcatt tggaattata
    34441 cattccatac ccagctgtca ccccaacatc aaagttagca ttttttgatc aggtatttgc
    34501 ttttcaccta gatgaagctt tttatgcagg aactgctttt ctgattattt tgctaattgg
    34561 ctggctgctt acccgatttg tgggggtttt tgtccatgga ttgacgtatg tgccgatctt
    34621 gagacaggta gactggattg caggaggcat cctgtcacta atcatggctt acgttactat
    34681 atttttgatt ttgcagttac ttgcctttgt tccattagtg tggtccaaaa tcaattcggc
    34741 gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa caagatgtat
    34801 gagctttggg taacgaatat tttgaattaa ggaaaatagt gctggaaaat atttcgtttt
    34861 ttgtacagag tagtttattt aagaaggctg cgtagcgtat gtccggcaca gctctttttt
    34921 catagtgaaa gataaggtga taaaatgaat aaccgaatcc ttgaaacatt ggaatttgaa
    34981 aaagtcaaac agatggtcag acaatttgta gtgactgcac aaggaaaaga agaactggca
    35041 gaattggtac cagtgagtga gaaacaaacg atcacaaact ggcttcaaga aactgaggat
    35101 ggattgaaag tgcaacgtct gcgaggcggg atccctatac ctaaacttga aaatatccgt
    35161 ccccatatga aacggatcga gatcggagca gacttgaatg gagtggagct tgcacaagtt
    35221 tccagagtat tgtctacgac aagcgaattg aatcgtttca ttgatgatct ttctgacagt
    35281 gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt tagtcacgat ccctgttttg
    35341 agccgccgtt taaaggaagc aatcgatgaa gacggacgcg tgactgatga cgcttctcct
    35401 gagttaaaga gcataagaca gaacattcgg cgaagtgaac aagctgttcg tgaacagtta
    35461 gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg atgcgatcat caccatgcga
    35521 aatgaccgct acgttatccc agtcaaacaa gaataccgcg gtgtatttgg cggtgtagtc
    35581 cacgatcaaa gtgcttcagg acagacttta tttattgaac caaaacaagt ggtggatttg
    35641 aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg agattcaacg tattttaagt
    35701 gaactttctg ctgaacttgt tccccatcgt caagaaatca tccacaatgc ttatgtgatt
    35761 ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta aagaagtaaa agccatcgtg
    35821 ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag caagacatcc actgatcgat
    35881 caggaaaaag tagtttctaa tgacatcaca atcggaaaag actatcaagc catcgtcatt
    35941 acaggaccta acacaggtgg gaaaacgatc acgttgaaga ctttaggtct gttacaactg
    36001 atggggcaag caggattacc gattcctgca ggtgaagaaa gtcaaatcgg tatttttgag
    36061 gaagtatttg cggatatagg agacgaacag tcgattgagc aaagtctatc gaccttttct
    36121 tctcatatga cgaacactgt agatatttta tcaaaagtca atgaaaaaag tctggtatta
    36181 tttgacgagc tcggtgcagg aacagatccg caagaaggtg ctgctctagc aatcgcaatc
    36241 ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa caacgcatta tcctgaattg
    36301 aaagtttatg gatacaatcg ggcaaatacg attaatgcca gcatggagtt cgatgtggat
    36361 acactaagtc caacgtatcg tttgctgatt ggcgttcctg gacgaagcaa tgcgtttgaa
    36421 atctctagcc gattaggtct tgatacagaa gttattgatg aagcgaagca gttgatgaat
    36481 gatgagagcc aagatttaaa cgaaatgatc acagatttag agaatcgccg gaaaatggca
    36541 gagaccgaat atctggaaat gcgtcatttt gtttctgagg cacaagaact tcacgatgat
    36601 ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa aagaaatgga aaaagcaaag
    36661 aaaaaagcaa acgaagtcgt gtcagaggcg gaagaaaaag cagaaaaaat cattgctgat
    36721 attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga aagagcatca gttgatcgat
    36781 gcaaagaccc aattagcaaa tctacatcaa gaagaaactt tgaagaaaaa taaagtattg
    36841 aagaaggcga aagaacaaaa aacgttgaaa ccgggagacg aagtgttggt tacgacttac
    36901 ggccaaagag ggacgctttt acgtaaaaac gggaatcagt ggcaagtaga aatcggtata
    36961 ttaaaaatga atgtgtccga agacgagctg acgcctgttg ctcctcaaaa agagccgaca
    37021 caacgagtta tccatgctgt acgatctgaa tctagtagtc acgtgccgaa tcaattggac
    37081 cttcgaggaa aacgatacga agaagcgcta agtgaggtag atcaatattt ggattctgcc
    37141 attctagctg gttatcctca agtgactatt gtccacggaa aaggaacagg cgcacttcgc
    37201 aagggaatta ctgattatct taaaaaccat cggagcgtca aaagctttga gtttgcaccg
    37261 gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta agtaaagctt actaaaggta
    37321 agcagatggt tcgctatcca agtatagaaa tgctatacta atcatagatt ttaaaaatgg
    37381 aggtctttga tatgtcacaa gtaattacag ataagacatt taatgaagaa acagataaag
    37441 gacttgtatt gattgatttt tgggcaactt ggtgtggtcc atgtcgtatg caagcaccga
    37501 ttttggatca gttggaacaa gaatatgatg aagaagaatt cagaattgct aaaatggatg
    37561 tggatgaaaa tccagaaact cctcaacaat tcggtatcat gagcattcca acattaatgt
    37621 tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt tcattcaaaa gaacaactac
    37681 gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa aataagctga gggtgggact
    37741 gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa ggttattcaa attaatattt
    37801 tttgagtata attacgttgt caggctggat tgtttttttg acagacatgc tataataact
    37861 ttaattgaat atgaagggga gtaccagatg aacgaacgaa tcaaaaacaa attagcttta
    37921 cttcctgatc agcccggctg ttacttgatg aaagataaaa atggaacgat catttatgtc
    37981 ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt ttactggcag ccataatacg
    38041 aaaacggaac ggcttgttag tgaaatcgaa gactttgaat acatcgttac agaatcgaat
    38101 atcgaagcat tgctcttaga gatcaatctg ataaagaaaa atgatcctaa atacaacatt
    38161 atgttaaagg atgataaaac ctatcctttc cttaagataa cgaatgaaaa atatccccgt
    38221 ttggtgatta ctcgtaaagt actaaaagac aaagcactgt attttggacc atatcccgat
    38281 gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat tatttcctct gagaaaatgc
    38341 aaacctagtc agacgaagga gccttgtctt tactatcatt tgggacaatg tctgtgtcct
    38401 tattatttcg acgtagatcc gaaagtctat acgaaaatcg ttgatgaagt gaaaaagttt
    38461 ttaaacggag gacacgaaaa aatcgagtca gagatccatc agaaaatgga aaaagctgct
    38521 gaaaacatgg agtttgaaaa agctgcagaa taccgagacc agatccgtgc cattgagaca
    38581 atcatgactc gccaaaaaat gacaaatact gatttactgg atcgagatgt atttggttat
    38641 gcgattgaca aaggatggat gtgtgtccaa gtgtttttcg taagacaagg aaagctaatt
    38701 gaacgagatg tttcaatgtt tcctttctat aacgaagcag aagaagattt cttgacttac
    38761 ctcggtcagt tttatcagga aaatgagcat ttcattccta aagaagtctt gattcctgat
    38821 acgatcgaca agcccagtgt agaagctttg ttagctacga aagtgctgca accaaaacga
    38881 ggagaaaaga aaaaattagt tgaattggcg aataagaatg cctcagttgc tttgaatgaa
    38941 cggtttgatc tgattgccag aaaacaggaa cggacgatcg gagcagttga acgtttagga
    39001 aacgcgatga atatcccgac cccaatacgt atcgagtctt ttgataactc taatatcatg
    39061 gggacgaatc ctgtggctgc gatggtcgta ttcattgatg gaaaaccggc aagaaaagaa
    39121 tatcggaaat ataaaataaa aacagtcaac ggaccggatg attatgcatc aatgagagaa
    39181 gtcatctatc gcagatattc acgagtgatt cgcgaagggt tgcctttacc tgatttgatc
    39241 ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag atgtcttaga aaatcaatta
    39301 ggaatcgata ttcctattgc tggcatggca aaaaacgata aacataaaac aaatgagttg
    39361 ctattcggta tagatttggc tgtagtaccg cttgagcgaa attcaccaga gtttttctta
    39421 ctccagcgaa tccaagatga agtccatcga ttcgcaatca ctttccatcg gcagctacgc
    39481 agtaaaaaca gttttgcttc ccgattagat gaaattgaaa ggttaggacc aaaacgaaaa
    39541 aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca cagcagccac gacagaagaa
    39601 ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg tatttgataa attacatcag
    39661 gaatgaggaa agggcacttt acaaagaaag taaagtgtcc tatcatttta tgtacaacaa
    39721 aaagagcgtt tacgttttgt tggtaaataa taaatcgctt acgtctggtg tctaaggata
    39781 tatgtgattt cttgaagaag gagtgaagaa atgttagaag tcaaggatct tgttaaaact
    39841 tttgggaact acacagctgt cgatcatgtc tctttccaaa tccccgatgg aaaaatcatg
    39901 gggctaattg gccaaaacgg ggctgggaag acaacgactt ttcgtttgat cttggatttt
    39961 ttgacacaag accaaggaga agtgttatgg aatggccatc atcttgggga gaaagattat
    40021 gatgtcatcg gttatcttcc ggaggaacgg ggactttatc cgaaagtaac gattcaagac
    40081 caactgcttt attttgcaga acttagaggg aaatcaagaa aagagattga accaaaaatc
    40141 gatttttgga tggaaaaatt tcaggtaaaa gggaaaaaga cggataaagt aaaatctttg
    40201 tctaaaggaa atcagcaaaa agtccagctg atcgccacgt taatccatga acctaaattg
    40261 atcattttgg atgagccatt cagcgggtta gatccggtaa atgctgaatt gctaaaagat
    40321 ggaatattgg aattgaaaaa acatggttca tgtgtcattt tttctagcca caatatggat
    40381 aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa acggaaaaat ggttttgaat
    40441 ggaaaagtac atgagattcg tgaatcattt ggccgtacga agttattctt ggaatctggt
    40501 ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa aagtgatcct tcatgaagac
    40561 aatacgttag agattacctt agaagatcct gaagctggga aagaaatctt tactcgtgcg
    40621 actcagttcg gctacattcc tatgtttaac cagcaaccgc caactttgga agagattttc
    40681 aaattgaaag caggtgaaat cgatgcgtaa gttttggatt attaccaaag atgtctattt
    40741 aaaaaatgtt aagtcgattt ctttcttgat catgatatta gtccctttta tcatgctggg
    40801 tatcatttat gtagcaggaa actttgctca acaaaatagt gatgtcaata aaatcggcgt
    40861 attggctgat aatgagcaaa tcagtcagca aatgagccaa gtcaaaacag atgattttga
    40921 gttcaaagca gttgattctg aagaaaaagc acaaaaagag ctttcggaag aaaaaatcga
    40981 tgcgtattta gtagtctcaa ctgagaatga taagataaat gggatgcttt atagcgaaaa
    41041 ttcattagga caaagtacac aactactgat acaacaacaa ctgactggct tgcaatcgat
    41101 gcttcgagca agtcaattag gcatttcacc agaagaagta gctagcttga gccaacaagc
    41161 aagtttgact cgacaaaaag tcaattttga ttcaaaagga aaaatgacag ttggagaaga
    41221 taactcgaca gtccaatatg tcgtaagtta tgtagcaacg attgtcttgt tcattattat
    41281 tttgacgtat gcgcagatca ttgcacaaga gatcgcttct gaaaaaggca cgcggatcat
    41341 ggaagtgatc ctgtccagta cgactgcaca aaagcatttt tacggaaaac taacaggtgt
    41401 tttacttgtt gcattaacgc aaatggcatt atacggtatt atttttggta taggatacag
    41461 tcaattcaaa gatatggaaa tggtcaaaaa tatattagac ggtatctcat tggataactt
    41521 gattggacca ttcttgtggt attctcttct attcatgatc tttggcattt tgattttttc
    41581 tgttttagca gctttatgtg gctctttggt caacaaagca gaagatacag caaaagcaat
    41641 tttgccagtg acttatctat ctctaggcgg atatatgctt ggattgatac taggagcttc
    41701 agatccaaac aatatcgtta ttcggatcac ttcgtatatt ccattcttat cttcatatat
    41761 tatgccggtt cgcttagcaa atgagacagt cggtatgggt ggagcattga tttccttgtt
    41821 ggtacttgta gccgtaacat tcttattgat gatcttgtct gcaaacatgt acaaatcgaa
    41881 tgtactcgtg tatagcgaag gcggattatg gagttcattg aaacaatcga tttcaattat
    41941 gagaaatgaa cgaaaaaaag cataaaatat agaaaaatat agaaggaagc tggacattta
    42001 ttgttcccag cttctttttt gtatagacct atttctattt ttttattata ttggatatat
    42061 atattcattg aaaatagagc ataacaactt tataatgttg agtataccaa tttttggagg
    42121 cgttttaatg gaccgctatt tagatttact ttcagagaag ttccctacaa cggaagatgt
    42181 catcacggaa atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt
    42241 tttgagcgat atacatggcg aatttcctgc ttttgatcat attctgcgta tcggttcagg
    42301 aaatcttaaa gaaaagatca aagacctttt ctctgaacaa atgacggaaa cggagatcag
    42361 ccaattcact atttttacgg cttatccaga atatgtgttg acgactgatt ggtacaaaaa
    42421 acaagataaa tcccagctga tccatcgttt gatcgatctt ttacggttta cgacagtcaa
    42481 atacactcga tcaaaagttc gaaaaggatt gccgaaagaa tatagctata tcattgagga
    42541 actcttgtat atcgatgatc gtatcaacgg gaaaaaagac tatgtaaaaa aaatcattga
    42601 acaacttgtc ttgatgaagg aagaagaaaa atttttaaca aaacttgcgc agacgatcca
    42661 aaaatctgtc atcgatcatt tgcatatcgt gggtgatatt tttgatcgag gaactcaagc
    42721 agccaaagta atggatcgaa tcatggattt ttcctctgta gatatccagt gggggaatca
    42781 tgatattttg tggattggtg cgtattgcgg ctctgaggca tgtttattga cgcttttacg
    42841 gattgccgca cgctacaact atttatttga attagagaaa gaatatggat taaatttacg
    42901 tccattattt tcttttgccc atcaaaccta tcagaagaat ccagtattta cccctaaaaa
    42961 caggaaagat ctatctgaaa gagagcaaga agaattagaa aagatccatc aagcactttc
    43021 tatcctccaa ttcaaatcag aacatcagtt gttgaatcga cgacctgaat ttaaaatgga
    43081 cgatcgtaaa ttgttggaaa aaatcaacta tgaggaatca acgatcgttt tagaaggtca
    43141 gctctatgag ttgaaaggga cttgttttca aacgatccag ccagatgatc ctaaaaaatt
    43201 cttggaagag gaacaaaaag tgatcgatag tttgatgtat tcattccagc actcgttaag
    43261 aatgcaaaaa cacatgacgt ttttgatcga aaaaggggga atgtatctca cgaacaatca
    43321 gcatgtactg ttccatggct gtatacctgt agatgaaaag gggcagctct tagcatttga
    43381 attagaccaa tcttatcgcg gaaaagaact gctgggattt tttgaaaaac agataactga
    43441 aagcgcaaaa gatttgactg caaatgaaga tttggcgacc gatttgattt ggtatgcgtg
    43501 gagcgggcca ttttcgcctt tattcggcaa gagtaaaatg gcaacttttg agcgctattt
    43561 tctcacagag cctcatacgc acgtcgaaaa aagcaatccc tattatcact tgcgagatga
    43621 agagtggttc agtgaaaaaa ttttggcaga attcgatgca aaagaagaag gttcagcaat
    43681 catcaatgga cacacgccag taaaagtaaa aaaaggagag tcaccgatca aagcaaacgg
    43741 aaaagcattt gttattgacg gaggtctatc caaagcttat caaaaaacaa ccgggattgc
    43801 tggctactca ttactatgca attcttacgg tttccaaatc gtgacacatt atccgttctt
    43861 gcctattgaa caacaatttg caaaaaaatc tgatcaaacg aatgtaaaaa aagtgatcga
    43921 gcagcagctc ccacgcaaac ttaatcggaa tacgacaaaa ggaatggaat tacagcaaca
    43981 gattactcaa ctccaaaggc tgttggatta tcgaaaggac gattgagaaa aatgctagcc
    44041 aacattgctc aatagactaa aaagcgactg caacaaaagg cgtgtcgcag tctccttcgg
    44101 aattagctca aaaaacggta aaatattaga agctattttt ctgtttattt ctctaatact
    44161 caggtcaggg cgctgatttc acgacctctt ccttaaacgg tgttctcctg ccaactcctc
    44221 ggtcacaagt cacaatatcc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg
    44281 actcagagtt gaacggcagg ctcacgacct aatcagaaaa acgattaaaa ttcattcaca
    44341 aagaaatcga ataaacgctg ttcagatgaa tctacacgat gagaaagttc cggatgccat
    44401 tggacaccaa ggattttgtg catattatct ttactttgga ctgcttcaat gagtccatcg
    44461 ttggaaaagg cgatggcttt taacaaagga gaaagatctt tgactgcctg atgatgatag
    44521 gaattcacaa agtatgaatc agggagaagg tcacttaata aactgtccga aacgattttg
    44581 acttcgtgag ttgcaaactg cggggcagtg ggctgttgtt cgtgttttac ggaccatttt
    44641 ggatataatg aaagatcttg ataaagtgtt ccctccaaca cgacattcaa tagttgaagt
    44701 cctcggcaga ctgcaaaaat cggttttctt tgtttcaatg cttccaagat caatgcctgt
    44761 tcgaattggt ctcgttgaat atttgtttcc gtcagttttg gatgagggtc ttccatgtaa
    44821 agttgcggac taacatcctg accgcctgca agaatgagtt tgtcgatttt ctcaattagt
    44881 tgaggcgctg aatctggaga catgactggc agaataagag gtaatccttg agcatctaaa
    44941 actgcatcca caaatccctg cggtgtatag cttacttgat ttccttggaa agtatctgtc
    45001 gctcgaatca gttgattccc ggcaatgccg atgattggtt tcatttttat tccccctaaa
    45061 gtagactaat tgtaacaagg atttaaaaaa atttaaacta tgtaagactt gcccctatac
    45121 ttatctgttc ttgctgtgtt actatctgta aagagaattt tcgtgtgaaa ttcatgacga
    45181 tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg
    45241 cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct
    45301 gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct
    45361 cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag
    45421 cagaagaaga aaatgagaag cgtgctcgaa ccctacggca gcagttgaag acctatgcag
    45481 aagaaaaaga gcaagaattg gagataatcc aaacgaacag tcggcaacag ctgaaacagt
    45541 tggtagaaga aaaagaacaa ttcgaaaagc aagaattaga aaaagaaaaa atgaaattga
    45601 aagcagaagc agacaaggtg caagcaactt tgaaagacca atatcaaaag catcagagtc
    45661 aagcaatcgg tgcaatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa
    45721 aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga
    45781 atccaaaatg ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtagaa
    45841 aaatattttc cagataccac gattattgat aaagaagcaa aattaagcgc tttaggcaat
    45901 cggctgaaag acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa
    45961 aagaaactcc atttgaaacg tcaagaactt tccttacaag acttggaagc aaattattcc
    46021 gaagaaaatt tcatgaataa attagaagaa gtccttgatt taaaagaaaa gtgggaacag
    46081 ctaactgaaa aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag
    46141 ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg
    46201 agtgcagcaa attttgaaag tttcaaggaa gaactaagta agcttgatga cgtttatatg
    46261 gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca
    46321 gttgctgcta tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat
    46381 gaacaaaatc ctaaagaaaa attgcaagaa accaaacagc aattatccat tgttcacgag
    46441 cagcaaaaaa agataacgac agcgttaggt cagtgcagcg gctatatcca agaatttgaa
    46501 tgggtagaag aagtcacact tgccgttgca gaacgggaaa aagtaaaaga acggtttatc
    46561 catgccactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg
    46621 atgcatgtgt taaacgaaac tgtatcagaa gaagatatat acgttagttt cgaaacacca
    46681 aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc gcttatcgaa
    46741 ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaacg
    46801 ccttggatga caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttac
    46861 ggattgctga tgctcattgg gacaatacta gctcaaaaac tatttgtttt accccgcggg
    46921 atgaaacgtt tcgtaaaatt ctttgagatt ctctcggtac catcgatcat ctggggattg
    46981 atctacagtt catttttcgg acaggcatta ccaaaagaac tatttggtat ccgcttgcct
    47041 ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc
    47101 ggtctgatac agatcttggt tggattattc gtggcagcaa aggaaaatat caggaggaaa
    47161 gattacttga gtgctgtcag tgatggtttt gcctggcaag ggatcctgat tgggatcgtt
    47221 attgcattga ttggtgtcat gttgataaaa aatacagcat ttgtttatct tggcgcgggg
    47281 ctatcgatca ttgctgcttt atgtatctta atcgtgccga ttatccaatc gccgtctaaa
    47341 gtcaagggtg ctgccaaagg agcgtataat ctttacggat tgactggtta tatcggtgat
    47401 ttagtcagtt atacacgttt gatggcacta ggtatttcag gcggaagtat tggcgcggca
    47461 ttcaatatgc tagttgcttt catgccgcca gccgctcgat tcagtgtagg gatccttttg
    47521 atcatcgctt tacacgcact gaatatgttt ttgacactgt taagtgccta tgtccatggt
    47581 gcacgattgc agtatgtcga attttttgga aaattttata caggaggcgg acgtgccttt
    47641 gacccgctga aaacggcgga aaaatacgtc aacatcaatc ataagaaaaa aaacaataaa
    47701 taattggagg atttttatac aatgatggat tacttaatta gtcaaaatgg tggaatggta
    47761 tttgcagtat tagcgatggc aacagcgacg attttttcag ggattggttc agctaaaggt
    47821 gttggaatga caggtgaggc agcagcagcg ttaacaacaa gtcaacccga aaaattcgga
    47881 caggctttga ttctgcaatt gcttccaggt acgcaagggt tgtatggatt cgttatcgcc
    47941 tttttgattt ttatcaactt aggaaatgat atgtcagttg tacaaggatt gaactttcta
    48001 ggcgcttctc tgccgattgc ttttacaggt ttgttttcag gtatcgccca agggaaagtt
    48061 gcagcagcag gtatccaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt
    48121 tttgctgcga tggttgaaac atatgcgatt ttaggtttcg ttatttcctt cctattagta
    48181 ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc
    48241 agatgaatga agcagcagag caagaaagag cggcgttaga gcaagaagag cgaatgaaga
    48301 tcgatcaaaa ctttgaacaa aaacgtacgc aaatcgaaac agaacatcaa aaacaaaaag
    48361 aaaaacaaat cgaattactt gaaaaaaaat accgtcagct taggaatcgc caacaagtag
    48421 aagtacgaca agaaaatttg aatgcgaagc aggaattttt acgacgattg tttgcagatg
    48481 caatagctga aatggagaat tgggatgaat ctgaacaaat ccagtttatc aaaaattcat
    48541 tgtattcatt acctttgaca ggcaaagtaa cattcattgc tggagaaaaa tcagcagctt
    48601 atctttctca gacattgctg gatgaatgga acaatgaact tccctttatg atggttttaa
    48661 gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata
    48721 attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca
    48781 atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat
    48841 tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa
    48901 ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga
    48961 cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg
    49021 gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gctatacttt
    49081 ccataatctg aaggttttga caaaagccga gatgacaaat caaaatctgg accatttgta
    49141 tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc
    49201 aaatgaactc cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga
    49261 gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg
    49321 acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga
    49381 cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat
    49441 gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga
    49501 taaagatatg gaagattaca cgaattttct attagagaca gattatgctg attttctgcg
    49561 ccctgtcatc cgtgagggaa atcttgatct cgtaaagctt gaacagttga aagatgatta
    49621 tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc
    49681 ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag
    49741 aagccatttc gaaccagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga
    49801 tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc
    49861 gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt
    49921 atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt
    49981 acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga
    50041 tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt
    50101 atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg
    50161 taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag
    50221 gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag
    50281 agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg
    50341 aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct
    50401 ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc
    50461 atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag
    50521 atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg
    50581 ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat
    50641 gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa
    50701 gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc
    50761 gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt
    50821 ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg
    50881 ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc
    50941 ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta
    51001 atacatcaaa catgccagta gcagctcgag aagcttctat ctatacggga atcacaattg
    51061 cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat
    51121 gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt
    51181 atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg
    51241 cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tccccgtctg
    51301 gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg
    51361 gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct
    51421 attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg
    51481 catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg
    51541 tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa
    51601 aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt
    51661 caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc
    51721 gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg
    51781 aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta
    51841 ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt
    51901 aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct
    51961 ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga
    52021 caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt
    52081 atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg
    52141 gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag
    52201 atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat
    52261 tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt
    52321 cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct
    52381 cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct
    52441 gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg tcaaacgggc
    52501 gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgaacgtatc
    52561 gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat
    52621 gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca
    52681 gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct
    52741 actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg
    52801 attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt
    52861 acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt
    52921 gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga
    52981 gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca
    53041 aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg
    53101 aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg
    53161 attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta
    53221 aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg
    53281 gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca
    53341 actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc
    53401 atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa
    53461 gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat cgaagaatta
    53521 tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta
    53581 aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac
    53641 gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt
    53701 ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc
    53761 gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa
    53821 acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg
    53881 atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa aagaggaaaa
    53941 cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag cttctcgcat
    54001 taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta
    54061 atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg
    54121 taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga
    54181 tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg
    54241 cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc
    54301 tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg gcctttgtta
    54361 tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt ggctggttga
    54421 aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt
    54481 tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg
    54541 ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc
    54601 agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat
    54661 taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta gtggaaggga
    54721 atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat
    54781 attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga
    54841 tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg
    54901 tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa gcatttggcc
    54961 gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt
    55021 tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca
    55081 tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat
    55141 tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac
    55201 gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa
    55261 aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct
    55321 agtgatttag taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg
    55381 caaaatgttc gtttattttt tgaggcacta cccgttgttg aacctcgggt agtgcctctt
    55441 ttttattgaa aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat
    55501 atttttttat tttttctttt acgatactta gtccagaagc taaaatttca aggttattag
    55561 aggataggga aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag cgtctggaat
    55621 gataaagtcg gataccgtta gcaagaaagt cattctcaat tatcttaggg tcaatctcag
    55681 gatcaactgg aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa
    55741 gagaatcaaa aagctgattg gctctttttg taagacttaa tttagtttct aagatcctat
    55801 ttgcttcatt tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca
    55861 catttatatt aaaaattgct tgttctatct tgctttggac atgtactgga actacaagat
    55921 aagcaactcg caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt
    55981 taggtaataa attttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg
    56041 aatggatatc atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct
    56101 ctcgtctttt gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga
    56161 caccatgaat cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct
    56221 cgtcaaaatc aattggaacg atctctaaat gatataactg agccaattca atgaaattag
    56281 cgtatgtata tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga
    56341 tagccaatcc attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc
    56401 cttgagtttt taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt
    56461 ggtaattaag caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa
    56521 cgaatttatt acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta
    56581 cttcattggt agagatggtg atggactcaa agacattggg agaaataaat gtgccacttc
    56641 caatattcgt atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag
    56701 taaaatttaa atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct
    56761 gtaatttatt ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac
    56821 gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag
    56881 gcattttctc cactccttaa aattgtatac catacaattt taccattgat tgtattgatt
    56941 ttagaagtta atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag
    57001 tagtagcttc ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt
    57061 atgaaagtgg taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag
    57121 gcaaaaatat gtttgcgatg atgcctgctt atttggggga gaacagatat tttggcacaa
    57181 aagtcattac cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga
    57241 ttttcttgtt tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca
    57301 cctggattcg aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg
    57361 cagaaacact tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt
    57421 ctaccattcg tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata
    57481 cttttatcgc taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag
    57541 tagaagatgc agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat
    57601 ttcttcaaaa agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc
    57661 ctgatacaag agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt
    57721 actcagctgc cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag
    57781 aggaatcttg tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata
    57841 aaggagaatc gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt
    57901 gtgcagaata tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta
    57961 cgtagttgat gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct
    58021 agaagaatgg ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga
    58081 aacagcattt acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg
    58141 agaaatcgat ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta
    58201 caaaatacct gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag
    58261 aattaaagat tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt
    58321 gtctgaatat gaagaagcga ttggggctaa gattaaagaa gcctacttag cacgtgattt
    58381 atttttcgta ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat
    58441 caaaaatttt gaattaggaa taggagtcat cgtgacagca gaaagtgtgc aaaaagactt
    58501 tgtttctaga accttctttc caaaattaac aatcaatgaa gatccagtat gtggatcagc
    58561 acattctaat cttattcctt attgggcaga aagaatgaat aaaacaaaat tgtcagctta
    58621 tcaaatgtca tcaaggggcg gggatctaga atgtgaatta aaagaagatc gtgtgataat
    58681 aggaggaaaa gcagttttgt tttctaaagg tacctcgttt atttgatttc tttcaaatga
    58741 attaaaaata aattggtgga gagtggcaac cttatagcta caatggttgt taaaaaaaga
    58801 cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg gtgtttgttt
    58861 tttattcaat caagagtaaa atgccccata ctaactatcc tcggatgact tcgattttat
    58921 aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat ccttttaagt
    58981 cagtaatctc aagaccggca gctttatgtt gctcgtgtaa tttttctaga tcatcagctg
    59041 ctatagcgat atgaccatag ccgtctccta gatcatacgc accatgatca tagttgtaag
    59101 tcaactctaa ttcatagtca tccccaggaa gtgtcaaata aacaagtgta aatttgtttt
    59161 ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc cttttgataa aattcgattg
    59221 atgcttcaag atctttcact cgaacacatg tgtgagccat tttcatagat tcatctctcc
    59281 ttttcctttt actcaatcat aacaaaaaga agtaaggaaa tagttgattt gtgcttgttc
    59341 atgagaaatt tctcatgtaa aatggataga aaataaaact gttaaggtgg aaactattca
    59401 tggataaacc aatttttggt gaaaaaaagc aatttgctga ttatcaaacc cgttatgcag
    59461 catatattgt catagaaaga gatcagcaga ttgcagtaat cgaggctcca aatggtgctt
    59521 tttttcttcc tggcggggaa attgaaggag aagagacgaa agaagaagcg attgtccgtg
    59581 agctgataga agaaatggga attgtggcag aaatttcttc ttatctggga gaagctgatg
    59641 agtattttta ctcgaattat cgtgcaactt attattataa tccaggatat ttctttgttg
    59701 ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac caataaaatt tggtgggtga
    59761 gccctcaaga agcgcttgtg aaattgaaac gtggtagcca ccgatgggca gtcgagagat
    59821 ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc agctttctct cagttttttt
    59881 atttattaca gtacatattt tttgattgct tcagctacac cgtgctcgtc gtttgatgaa
    59941 gtgatgacat ttgctgcttc tttgacaaga gaaacggcat ttcccattgc tactcctaga
    60001 ccagcatatt caatcatgga taagtcgttc tcgttatctc cgatagccat gatttcatct
    60061 tgatcaatac caaggtgctg tgcgagattc gcaactgccg cacctttact tgcttcttta
    60121 tttagtactt cgaagtaata gggcgcactt ttgaccgttg tgtatttatc acggaaagtt
    60181 tgcggtaaac gcgcaattgc tgcatctaaa atttctggtt cgtcgatcat catcattttg
    60241 acgatgttca tgtctggtgt catttcttca ggtgttcgat attttagcgg catcttgact
    60301 aaacttgctt cgtggatcgt ataagcgctg atatctcgat taggcgtgta gatcgctttg
    60361 tcatcgatcg aatggagatg actgccgaca cggcgtgcca tcacttcgat ctctaggaaa
    60421 tcttcatgtg tcagtccgtg acgggcgatg attttacctg atttcgtttg ttgtacgagt
    60481 gccccgttat aagtaataac gtaatcatta tcttgaaaaa ggttcaactc gtttaactgt
    60541 tcctggacac ctggtaaggg acggccagtg cataaaacga tcgaaacacc ttttgcaaca
    60601 gcagcggcaa tcgcttcttt tacttctttt gttacttctc ttttctcatt taataaagta
    60661 ccatcgatgt cgatagcgac tagtttgata gacataattt cctccaatat attttaattt
    60721 atttcaatca atgcgccatt acggatatgg ctggcaaatt cttgataagt ttcgtcaaat
    60781 aaatcataat gatcacgcaa ggacggatca accatttctt ttggaaagaa aaatcgctca
    60841 tctccttgtt cttgtcccgc taatgcagca acaagctgac taacctgtga taattctatc
    60901 aaagttccgt catttcgcaa aatctcgatt tgtgtgcgat gtctgccttg ttctgggcga
    60961 taaaaatcat aaggtagatc gtagctagaa ttcacagcag tgtaataaac aggattatat
    61021 ccgacctttt ctactaacaa agtgagttct tggactaatt ttgaatcccg gttgccagaa
    61081 aaagtagcag atttcagtgg tttacgattc aaaaaacgtt tagctaaatc atttaaaata
    61141 ggatcagcag attcagacca ttgagtaaag taagtgccca agaccccatc atcaagtttg
    61201 aggtattctt ctaaagtaaa gcttccgtca agaaatggca gaagtaattg agaatggagt
    61261 tcaaaactgc tggcatcttc ttgatacaac tcgtgtgcgc gatgaagcag atgctccaaa
    61321 attacttcca taccacggga aacaggatgg aagtacactt gcacatacat ttgatatcgg
    61381 ctaacgatat aatcttccac tgcatgcatg ccactcatgg aaaaagcgat cccgccttca
    61441 taaggacgaa tcacacgtaa tattcgggta agatcaaatg ttccatattc tgttccagtg
    61501 aaataagcat cccgaagcaa atagtccatt cgatctgcat cgatctgact agaaatcatt
    61561 tggacaactt gtgggttggg gtaagttttt tggatcacac tagctacctt ttcaggaaaa
    61621 ccttcttcta ctcggttaag aatctgatac acttctgttt ctggagaagt gatgatctct
    61681 acagtgatgg cttcatgatt cgtatggaag atatgttcaa atgtatgaga gtaaggacca
    61741 tgaccgacat catgcaacag tgccgcacat aaagtcacca gccgttcctg atcatcccag
    61801 cctccgttgc ctattttttc ttttgaaaaa tttcgagaaa agatatcgca gattcgtctt
    61861 gaaatctcat aaacgcctaa tgaatgagta aaacggctat gttctgctcc gtgaaaagtg
    61921 aaagaagaag tgcctaattg tttaatgcgt cgtagacgtt ggacttcttt cgaattgatc
    61981 aaatctaaga ttacttgatg ctggacatgg atatagttat ggactggatc acgaaaaact
    62041 ttttccatag gcagcatttg atatttataa ggggttgtca taatgtagct cctttatgtt
    62101 attacgttgt tccattctct ggatttggtt gttccattgc tccagctgga tttctttttt
    62161 tagccagtcg gtagaatcct gtaaatcact tgcagaatac agcttttcaa aagcttttgc
    62221 aaatgcttct ttgacttctt ttacaaaaag gggttggtca agcagttctt caagtgttgc
    62281 cattgtagca ggatccactg caggatagcc atttgtacca aagtcttctt ttaaagcaga
    62341 ttgataaaat cgcttcatga cttgcccgcg tttttcctga ttaccgttga cacttaagta
    62401 catcataaca gaaatcccat ttttgatccg acgctgagcg ataccggcaa attttttgcc
    62461 atggatgctt aaatcataag ttccaggaca ataagaatgg gtgatttcga atgcttgtat
    62521 cggtgccttt ggaaaagcgt atttagtcaa taggagcatt tgctcgtaag cgtcatctat
    62581 ggttattttt tttgtgtcac tttgggggaa aatcaaagaa atattcaaaa ttccttcatc
    62641 tgatacgatc cctaatccac ctgaatttct gatcactgga gcgtaacctg catctttcag
    62701 tgtttggatc ccgtcactta gaaaaggagt tcgaacatcc ttcataccta agatgactgt
    62761 gcagtcatat tgccagaaat gtaaaaagat ttgctggtgt tctttagaat attccgttaa
    62821 tatatctgtt aatgcaaaag gggaaaagtc gtttttttga taaatgtgct gatctagtcg
    62881 aatcgcaggg ttcattcaac cggctccttt gtataaattc atattttcta caatattata
    62941 ctctaaaatc aaagagttgt tttggaaaaa aggttttctg taaaaatgga caagttggtt
    63001 gacaaaacaa aagaaactag gaacaatgta gctatgagaa gtaaaggaga atgaataatg
    63061 gaattatcac aaggaacacc gatttcaatc aaattgcgta caaaagtaaa acagcaaggg
    63121 gaagtgcaag atttttattt tgatttgaaa gggcaaatgg tgacaattgg cgatacgctg
    63181 tacatacgtt ataaagagat tcaggaggag acgggcgatg agattcctgt aacgatcaaa
    63241 ttagttccag atggtcatgt acagctgatc cgtgcaggag aaatgcggat gcgtttgaaa
    63301 tttggctata aagaacggtt agaaacatcc tatcgaacac catacggtat gatccagatt
    63361 gctactttta cgaaagaact gcatgttagt ttgaaagacc gtcccacagg agggaaggtt
    63421 cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg gcgaatatta tttgaccttg
    63481 gattttacag cgtaaatata aaagaaaagt ttgatttttt gtgtctgatt ttgtatgata
    63541 aggagtagac tgtgaaagga cgtgtcctga ttggaaatta gtgtatttga aggtgcaaac
    63601 aaaagcgaat tatcaatgat cgaagtagca cacgcgatct tagaacaacg tggcgatgtc
    63661 atggattttt ctgatttagc taaccagatc caaaactatc ttgggaaatc agacagtgaa
    63721 attcgtgatt cactcgcaca attttatact gacttgaata ttgacggcag ctttatctca
    63781 ttaggggata atcgttgggg cttgcgttca tggtatgcta tcgattcgat cgatgaagaa
    63841 gtaaaccatg gaatggacga agaggatgaa gatactccac gtcgccgtaa acgcaagaaa
    63901 gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt acaatgacga tgacccagaa
    63961 gatacagatc tgacagaaga agatgacgat gatctgtttg atgacgatga tgatgaagat
    64021 gaagaaatcg ctgcttataa ttctgacttg caagaaatcg gagcagatga cccgacagat
    64081 gatgaagaag acttgccagg aaatatcgaa gaagatttaa gtatcataga cgaagacgat
    64141 gatgatgaag attatgaaga ggaagaattt tctgactctg aaaaagaata aacattattt
    64201 tcttacgaga ggttgtgaca gagacgatca gcctatcaga ataaggaggt gtgacacgat
//
LOCUS       DANKAZ010000003        60875 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000003 DANKAZ010000000
VERSION     DANKAZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="PWY67_000176"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="PWY67_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL2056489.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="PWY67_000177"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="PWY67_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL2056490.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="PWY67_000178"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="PWY67_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL2056491.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4735..5628
                     /locus_tag="PWY67_000179"
     CDS             4735..5628
                     /locus_tag="PWY67_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2056492.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="PWY67_000180"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="PWY67_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2056493.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="PWY67_000181"
     CDS             7565..8422
                     /locus_tag="PWY67_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL2056494.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="PWY67_000182"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="PWY67_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL2056495.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="PWY67_000183"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="PWY67_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL2056496.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="PWY67_000184"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="PWY67_000184"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2056497.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /locus_tag="PWY67_000185"
     CDS             11178..11936
                     /locus_tag="PWY67_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2056498.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="PWY67_000186"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="PWY67_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL2056499.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12864..13895
                     /locus_tag="PWY67_000187"
     CDS             12864..13895
                     /locus_tag="PWY67_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2056500.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13892..14659
                     /locus_tag="PWY67_000188"
     CDS             13892..14659
                     /locus_tag="PWY67_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2056501.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="PWY67_000189"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="PWY67_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL2056502.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="PWY67_000190"
     CDS             16624..16944
                     /locus_tag="PWY67_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL2056503.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="PWY67_000191"
     CDS             16944..17294
                     /locus_tag="PWY67_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2056504.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /locus_tag="PWY67_000192"
     CDS             17461..18399
                     /locus_tag="PWY67_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL2056505.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /locus_tag="PWY67_000193"
     CDS             18414..19244
                     /locus_tag="PWY67_000193"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2056506.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="PWY67_000194"
     CDS             19288..20313
                     /locus_tag="PWY67_000194"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2056507.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="PWY67_000195"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="PWY67_000195"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2056508.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="PWY67_000196"
     CDS             21391..21831
                     /locus_tag="PWY67_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL2056509.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="PWY67_000197"
     CDS             21834..22436
                     /locus_tag="PWY67_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2056510.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="PWY67_000198"
     CDS             complement(22495..23601)
                     /locus_tag="PWY67_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2056511.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="PWY67_000199"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="PWY67_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL2056512.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="PWY67_000200"
     CDS             complement(25149..27494)
                     /locus_tag="PWY67_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2056513.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="PWY67_000201"
     CDS             27773..28675
                     /locus_tag="PWY67_000201"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL2056514.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="PWY67_000202"
     CDS             28888..30663
                     /locus_tag="PWY67_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL2056515.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="PWY67_000203"
     CDS             complement(30729..32315)
                     /locus_tag="PWY67_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL2056516.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="PWY67_000204"
     CDS             32566..32790
                     /locus_tag="PWY67_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL2056517.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="PWY67_000205"
     CDS             32932..34683
                     /locus_tag="PWY67_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2056518.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="PWY67_000206"
     CDS             34683..36467
                     /locus_tag="PWY67_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2056519.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /locus_tag="PWY67_000207"
     CDS             36793..37644
                     /locus_tag="PWY67_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2056520.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37698..39449
                     /locus_tag="PWY67_000208"
     CDS             37698..39449
                     /locus_tag="PWY67_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2056521.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            39453..39623
                     /locus_tag="PWY67_000209"
     CDS             39453..39623
                     /locus_tag="PWY67_000209"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2056522.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(39663..40565)
                     /locus_tag="PWY67_000210"
     CDS             complement(39663..40565)
                     /locus_tag="PWY67_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2056523.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="PWY67_000211"
     CDS             40796..42199
                     /locus_tag="PWY67_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2056524.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="PWY67_000212"
     CDS             42199..43632
                     /locus_tag="PWY67_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2056525.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="PWY67_000213"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="PWY67_000213"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL2056526.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="PWY67_000214"
     CDS             44917..46644
                     /locus_tag="PWY67_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL2056527.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="PWY67_000215"
     CDS             46659..47009
                     /locus_tag="PWY67_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2056528.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="PWY67_000216"
     CDS             complement(47101..47961)
                     /locus_tag="PWY67_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL2056529.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="PWY67_000217"
     CDS             48053..48481
                     /locus_tag="PWY67_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2056530.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48643..48819
                     /locus_tag="PWY67_000218"
     CDS             48643..48819
                     /locus_tag="PWY67_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL2056531.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48846..49292
                     /locus_tag="PWY67_000219"
     CDS             48846..49292
                     /locus_tag="PWY67_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL2056532.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="PWY67_000220"
     CDS             complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="PWY67_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2056533.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            51531..52502
                     /locus_tag="PWY67_000221"
     CDS             51531..52502
                     /locus_tag="PWY67_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL2056534.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            52526..54718
                     /locus_tag="PWY67_000222"
     CDS             52526..54718
                     /locus_tag="PWY67_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL2056535.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            54735..55211
                     /gene="ybeY"
                     /locus_tag="PWY67_000223"
     CDS             54735..55211
                     /gene="ybeY"
                     /locus_tag="PWY67_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL2056536.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            55195..55590
                     /locus_tag="PWY67_000224"
     CDS             55195..55590
                     /locus_tag="PWY67_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL2056537.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            55605..56504
                     /gene="era"
                     /locus_tag="PWY67_000225"
     CDS             55605..56504
                     /gene="era"
                     /locus_tag="PWY67_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL2056538.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            56647..57459
                     /gene="recO"
                     /locus_tag="PWY67_000226"
     CDS             56647..57459
                     /gene="recO"
                     /locus_tag="PWY67_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL2056539.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            57843..58760
                     /gene="glyQ"
                     /locus_tag="PWY67_000227"
     CDS             57843..58760
                     /gene="glyQ"
                     /locus_tag="PWY67_000227"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL2056540.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            58762..60837
                     /locus_tag="PWY67_000228"
     CDS             58762..60837
                     /locus_tag="PWY67_000228"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL2056541.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        19863 a        10760 c        13027 g        17225 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc
    48541 aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata
    48601 acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt
    48661 cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg
    48721 ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag
    48781 aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg
    48841 gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag
    48901 ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta
    48961 aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac
    49021 agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag
    49081 taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa
    49141 ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg
    49201 gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga
    49261 atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc ggacagtgat
    49321 tgtccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa gaaagaataa
    49381 gattctttct tctagctgtt tttttcctgt ttaatatctc ttatgttaaa ttgtgtttga
    49441 tttggttatt cttctggtag ttcatttgct tcttgtttat accctgatag gcgataggcg
    49501 taataaaaga gtaagctgcc agcgtaacca agtaacgttg ggaccatcca gccaagtcct
    49561 aatgcagcaa aaggaacgtg atgatgataa aatcctagta agcttgctac gacagtttgc
    49621 tgactcacta atgcaggcga atttgccaac atatccaata cggctggaac ggcagaaaag
    49681 aacatcgtta cttgatagac aaatggactc ttttggaaga attttgctgt taaggaaagc
    49741 aagattaacg caagtgacaa tggatacaaa agcatcaata ctggtaaaga ccattggatg
    49801 atattatcca atccggcatt cgcaacgaca aaagagacaa aagtcgttaa ccgcagccat
    49861 gtcatataac tgactttagg gaacagttta tggaaatctt gtgcaaaaga aacaacgagt
    49921 cccatagcgg tagtgaagac acctaatgtg accattaccc ctaagaagct agcaccgaag
    49981 ttacctaaat aaaaattagt gatctgagaa agtgcagttc cgccattttc tgataaaggc
    50041 atcttattca aactcatcgc accgagaagg accagaccaa aataaatcat gacttctaac
    50101 gcaacgctca ggctaccagc ttttgctgtt aattctgtga cttttttatc tttaaaacca
    50161 agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac tgcatcaacc
    50221 gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg tctgataaga atctgcagca
    50281 gattggttta gattccccat gggttggaat aaagaaacga caaaaatgat agctaataat
    50341 gcaagaaaca aggagtttaa atactttccg acatatttga ccaaacgtgt tggatgtaca
    50401 gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata aaaacatacc taatgaatca
    50461 tatttttcag gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc tgtccgcggt
    50521 gtaccaaaga atggtccgat cgttaaatga acaaggatca agaacaaggc agcataccat
    50581 tttccgacag gtttagctaa gtcatacaga ccatcacttt ttgtcactac tacagctaag
    50641 atagctaata atgggaaaag agcgcctgag atggcaaatc ctacaccagc gctgatccag
    50701 tttgatccag ccatttgacc taagtggaca ggaaagatca gattacctga cccgaagaac
    50761 atcccgaaga taagtgaact gaccatcaat aattgcttcc atgaataagt ttttgtttct
    50821 tccataaatt tttcctccaa taattatcat tttgagtaac aaaataaacg ttgcagaaac
    50881 tagaaatgcc actaatatca gattccatga aaaccaccac cttaaaatat aaaaaaaagc
    50941 ccttttccac ttgggaaaag ggctctagat tctagaacgg taccaccttt tttcgcttaa
    51001 aaaagcttct tcattcacgg acgcttttcg ataaagttat ccgctagcct gataatggag
    51061 gcagccacaa tgatctacgt tttcgacatt gactcggaaa tgatgttcgt ctattcgatt
    51121 tattgtcatt tctcacttac atgactcact gggaataaat cgctgaatag cctactgttt
    51181 tcctcgacgc tttgctgtat atttatttgt tgacgttata atacacctgt tttttttgaa
    51241 atgcaagaat atttcaaata ttttgttttc tttttttgaa acaagaaaaa tacccagaga
    51301 atacttgcta agttagacgg aatacttgtc ctctatctta tgcatagctt acatgatcag
    51361 ctttttttat tcaaacgaga cctttagaga atggaagcag ctatttttta acgtttcttg
    51421 gtacttatgg ttaagcaact cttttagaac cttttcttat ttgctatata gtttcttctg
    51481 agtatggtaa aataggaaag aatgaatcga aaaaaagggg atgtctattt ttgacggaag
    51541 agtctagttc tttagagatt agattgacgg ctgctgatga tgtcagcatg cttttaggtg
    51601 cacacgacaa gcatataaaa ctgattgaag aaacaacaga aacaaccatc catacacgtg
    51661 gagaaatcat tcagatcatt ggcgaacaat cccagatttc cttggctgcc tcagtcattc
    51721 ggacattgca ggaattgatc aaacgaggaa tccatgtaag tacaccagat gtcgtgacgg
    51781 cactgaaaat ggggcaaaaa ggcacattag attattttgt tgaaatgtat gaggaagaga
    51841 tcgtcaaaga ccgtaatgga aaaccaatac gggtaaaaaa cagcggccaa aagaaatatg
    51901 tcgaatcggt agccaaacat gatatcgtat ttggcgtagg accagccggg acaggaaaaa
    51961 cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg caaaaaatca
    52021 ttttgacgcg gcctgctgtg gaagcagggg aaaatctagg ctttcttccc ggggatctaa
    52081 aagaaaaagt agatccttat ctgcgtcctg tttatgatgc tttataccaa atttttggat
    52141 tagatcatac gaaccgattg atggaacgag gcgtgatcga aattgctcct ttagcttaca
    52201 tgcgggggcg gacattggat gatgcattcg ttattttgga tgaagcacag aacacgacag
    52261 ttgctcagat gaaaatgttc ctgacacgtc ttggttatca atcaaaaatg attgtcaatg
    52321 gagatacgag tcagattgat ctgcctcgag ggacaacaag tggtcttgtt catgcagaac
    52381 gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc
    52441 ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa ggcagatctc catcaagaat
    52501 aagctttgct taggaggata agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa
    52561 acttggaaag gcagcattgc ctttcctgct gttcttgttt tttatcatta tctgcggcgt
    52621 tacatttagt agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag tagcagaaga
    52681 aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa aaagaaaact
    52741 ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa gatcttgcag atgaccagaa
    52801 caatcggatc aagcaactat ttgaattgat cgataaaacg aatgatgcca tcaacaaatc
    52861 ttatgacgaa aagattgata aggcaaagga agatgaaaat atcccaaagc ctactacaga
    52921 agagcggatc gccgcgttga agaaaaattt tgaaaatgtg aatgcagaaa atgttgcgtt
    52981 ttatcaaaaa ctaccgaaca atttttattc gacgatattt gaaatgactg aaactgagat
    53041 ccatactgtg agggatgaga gtctgaagct cattgacgaa cagatgagca aacaagttcg
    53101 tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag caaatccaat atttgaatgt
    53161 cacaccagca caacaacaaa tgattcgcta cttagtggat caaggtatcg tagtgaatga
    53221 cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag ctgtacaacc
    53281 agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg atgcttcagc
    53341 tatgaaaaaa ttggaattac taggactgac taatcaaaca acttcgattt ttccgctaat
    53401 tgccatggtt ttagctgttt tgcttcaaat cggcgtactg atttataatt cacttcaatt
    53461 ccaaaatgca ggagaaagaa caaaatacgt gcttttctat gtgacagcta tgtcagtcag
    53521 catagtgctg atgaaatttt tccaattatt ccaaacggaa caagctactt atatcccgct
    53581 gttttatccc gctgcttttg caccacttgt tttaagcttt ttcttgaacc gaagagcagg
    53641 gatcatggct ggtattttcc aagcagtatc tgcacttttc attttttatg attcgattgg
    53701 cacgaacttt ctaacgatta tcttgatgtc ttatgttttc tctgggctca tggcaacgat
    53761 tgtcagacgc aaacggatct cagaacaagg attatctgct gccatgtggg tcattatttt
    53821 tcctgtgatg atggatgtca ttttgatcgt ctatcaagga atgagctttg gtgatgcaac
    53881 gacttggtta atgcttgtct gcgggttcac tggtgcactc ttctcattct tgctgacgat
    53941 cggtctacac ccatatattg aattgatggt caatgatgac agtgtaattg tattaaatga
    54001 actcagcaat ccaaatcatc ctttattgaa acagttgctg gaagaagcgc caggtactta
    54061 tcatcatagt atgatggtag ctaatctaag tgctaacgct gtagcagaaa tcggcggacg
    54121 ttctttgcta actcgagtag cttgctatta tcatgatatc ggcaagatca aacatgctag
    54181 ctttttcgtt gagaacttgc cttcaggagc ggaaaacccg cacaatttct tattgccaga
    54241 agacagtaaa catattatct ttggacatgt gacagatgga gccaaaatat tggaagatta
    54301 taatatgccg caaatggtga tcgatatttg caaacagcat catggaacca cgctaatgcg
    54361 ttatttctac atcaaagcga aagaacgcaa tccggaagta acagaagagc aattccgtta
    54421 tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg acagttgtga
    54481 ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa
    54541 tcttatcgaa gagcgtattt ccgatggtca attagacgat agtggactga ccttgaaaga
    54601 aatccgaaaa gtggaaaaat cattgatcag cggtttaagt agcaccttcc attctcggat
    54661 caaatatcct aaaatgaaaa cagaagttga gaagataaag gaagaacaag aagaatagaa
    54721 agaagcgatg tataatggat attaccttta tggatgaaac aaaccaagtc tcagaagaga
    54781 aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg
    54841 aaacagaaat gtctgttact ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt
    54901 accgtggaaa agatgcaccg acagacgtca tcagttttgc tctggaagaa gaaggcgaag
    54961 acgaaatccc agtgattttt gaagatggag agaatccatt gcctcgtgta ctaggagaca
    55021 tcatgatttc tattgaacgt gctgctgaac aagcagaagc ttacggccat tcatatgatc
    55081 gggaacttgg ctttttagcg gtacatggat tcttgcatat caacgggtat gaccatatga
    55141 caccagaaga tgagaaagaa atgtttggtt tacagaaaga gatcttggat gcctatggac
    55201 tcaaaagata aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga atttgctgtc
    55261 caaggagtca aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt
    55321 atccttgctt tatttatggg cgtgattttc cagcttgatc ggatagagtg gttatggctg
    55381 cttttagctg tttttctcgt ttggatagta gagatcatca atactgtttt tgaaaatgta
    55441 gtggacatgt ttacggattt tcattttcat ccgattggga aaaaaatcaa agatatggca
    55501 gctggagcag ttttagtgac cgcttgtttt gctgtcatca ttgggttgat tttatttgtc
    55561 cctaaaatat atcatttgtt gtttaattaa aaaggagaac gtatatgcca gaacataaat
    55621 cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa atccacatta ttgaaccgaa
    55681 tcgtgggtca aaaaattgcc attatgagtg acaaggcaca gacaactaga aataaaatcc
    55741 aaggagtata tactacacca gaagctcagc tgatttttat cgatacacca ggaatccata
    55801 aaccaaagca tcgtctagga gattttatgg tagaagcagc ttacagtgcg ttgaaagaag
    55861 tggatgcagt cttatttatg atcagtgctg atcaaaaacg cggccgggga gacgatttta
    55921 ttattgaacg tctaaaaaac gtgcattctc cagtttatct gattatcaat aaaatcgata
    55981 agatccatcc agacgagttg ctgggaatca tcgaagatta ttcgacgcag atgccttttg
    56041 cacaagtgat tccgatctct gctacagaag gaaacaatgt cgaacgctta atggatgtat
    56101 tagtcagtga aatgccagag ggcccacaat attttccaga tgatcaagtg actgatcatc
    56161 ctgaatactt tatcgtttct gaattgatcc gtgagaaagt cttgttttta acacgagacg
    56221 aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa
    56281 tccatatcca ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc attatcggca
    56341 aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt
    56401 taggagacaa agtatttcta gaactttggg tcaaagtcca aaaagactgg cgagacaaaa
    56461 aagtctatct gcaagacttt ggctaccgaa aagacgaata ctgatattct gaacttctta
    56521 taggttaagt atagaaataa actggataaa aatccgcacg ttgagaaaaa cggcggaaga
    56581 tcgaaacata aataatctgg atcggcagaa tctgcttttc cagatttatt ttttaggagg
    56641 gagaatatga gccagcttgc agaatcgaaa ggcttgattt tatctagccg taattacaaa
    56701 gaaaaagata aattagtcaa aatctttacg gaatcttctg gcaaaatgat gttttatatc
    56761 aaaggaatcc atcggcaaaa tcagccatta gctccggctt tgctcccctt taccgaagca
    56821 gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc
    56881 catgcgtttc ggaacatcca agaagatatc tttttgagcg gttatggtac ctatttgcta
    56941 aatctagtag atgcagcaat cgaagatcat caatatgacc cgcatttatt ccaatttacg
    57001 caacaagctc tgcagcggat ggatcaagga gatgatgcag aaatcatcac gaatatcttt
    57061 gaagttcaaa tactgcaacg cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc
    57121 ggggaaacaa aaggaaaatt tgactattct tccaaatctg gcggtgtgat atgcgagaaa
    57181 cactggccaa tggacgaaca ccgttatcat gcagatcctc gcgctgttta tttcgttcga
    57241 atgttttcgg ctatttctta tgataagatc agtggaatcc acttgaaaga agaaacgaag
    57301 caggcaatcc gtgacctcat tgaccagttg tatgatgaat atgtcggtat tcatctaaaa
    57361 agcaaaaagt tcattgatca aatgaagtct tgggcacacg tattaaagac gccagagaaa
    57421 aatatgcctg aaaaagagat acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa
    57481 tcaaaactat ttgacaaata cagggacttc cgtctattat taaccgtaga ttaaatagat
    57541 cgttcactga gaggatctat tatataaaca agaaaactgc aaaatgaata cgtacattga
    57601 ccaagatcag taaaaaacac gtggtttagc gagttcggga gagtggaagc cggacccaaa
    57661 cgatttttga agaggcactt gcgagtacga taaatgaagc tgccgaatct ttattcggaa
    57721 gtagggtgga accgcgaaat tattcgtccc tatgtctgta atcagacata gggacttttt
    57781 gtcgttttct ttcctgtatt cttattgaca gcaacgctta aaaattgaaa ggggcaaaac
    57841 agatgagtaa aaaattgacc gtccaagata tgattttaac attacaaaaa ttttggtcat
    57901 caaacggctg catgctgatg caagcttatg acacagaaaa aggagcaggt acgatgagcc
    57961 catatacatt cttacgggca atcggaccgg aaccatggaa tgctgcttat gtagagccat
    58021 cccgccgacc agctgacggg cgttatggag aaaacccaaa ccgtttgtat cagcatcatc
    58081 aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca agagctttat ttagaaagct
    58141 tgcgtctttt aggaattgat ccattagaac acgatattcg ttttgttgaa gacaactggg
    58201 agaacccatc gatggggtgt gccggtgtgg gttgggaagt atggcttgat gggatggaga
    58261 tcacacaatt cacgtacttc cagcaagtcg gaggactacc ttgtaaacca gttacttctg
    58321 agatcactta tggactagaa cgtcttgctt catatattca agaagttgaa agtgtctatg
    58381 atctggaatg ggctgatggt gtgaaatatg gcgagatctt caaacaacca gaatatgaac
    58441 attccaaata ttcttttgaa gtaagtgacc aagacttgct actaagcaac tttgaccgtt
    58501 ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt tcatccagcg tatgattata
    58561 ttttgaaatg cagccataca ttcaatttat tagatgctcg cggtgcagta tccgtaacag
    58621 aacgagcagg ttatctttcc cgtatccgca acatggctcg tgctgtagcc aaaatctttg
    58681 tggcagaacg cgaaaaatta ggattccctt tattgaataa aaacacagca aatccagaaa
    58741 acatgaagga ggctgaataa gatggcaaaa gacttattac ttgaaatcgg gctggaagaa
    58801 atgcctgctc atgttgtcac accaagcatg aaacaattag aagaaaaaac agcaaaattt
    58861 ttagatgaaa accaattgac ttatgatacg atcgaaacgt tttctacacc gcgccgctta
    58921 gcaatcaaag taacaaacat tccagaacgt caagcagaca gtgaagaaga agtcaaagga
    58981 ccagccaaaa aaattgcttt ggacgcagat ggaaactgga cgaaagcagc tgaaggattt
    59041 gtccgcggac aaggtctgac gacagatgct atcactttta gagaattaaa aggcgtggaa
    59101 tatgtctatg taacgaaata cactcacggc aaagaagcga aagaagtatt gacaggttta
    59161 gcagatgtca ttaaaagcct cactttccca gtaacgatgc attgggcaga atacgatttt
    59221 gaatatatcc gccctattca ctggatcgtg gcattgttag atgacgaagt aatcccgttt
    59281 gagatcttag atgtcaaaac aggcagagcc tctcgaggac atcgtttctt aggcgaagat
    59341 gtgacatttg cgcatgcaaa agaatatgaa gaaaaattga atgaacaatt cgttattgct
    59401 tcaccgcaaa aaagacaaga aatgatcgtt acacaagcaa atgaattagc cgaaaaaaat
    59461 cactgggtta ttgatctaga cgaagattta ctagaagaag tcaacaattt agtcgaatat
    59521 ccaactgtct tttctggcga cttcgaagaa aaatatcttt ctgttcctga agaagtacta
    59581 gtcacttcta tgaaagaaca tcaacgttat ttcgaggtac ggaacgatca aggtatgctg
    59641 cttcctcatt ttatttctgc tcgtaatggt gatcgggtaa aattggaaaa tgtggttcga
    59701 ggcaatcaaa aagtattgac ggctcgttta gaagatgcgg aattctttta taatgaagac
    59761 aagaaagctt caatcgacca atacgttgaa aaactaaaaa ctgttacttt ccatgaaaaa
    59821 atcggtagta tctatgagaa aatgcagcgg gtacaagtca ttgcccgtat cattggcgaa
    59881 caagtaggac tatctgagga agaattgatg gatctgcaac gagctgctga aatctataaa
    59941 tttgatctag taaccaacat ggtaggggaa tttcctgaac tacaagggat catgggcgaa
    60001 aaatatgctc tgctgaaagg cgagaaacct gctgtagcaa cagcgattcg tgagcattac
    60061 atgcctatat ccagtgaagg agagctacct cagtctacag taggttctgt tttagcgatt
    60121 gcagataaat tagatagcat tttttctttc tttgcagttg ggatgattcc aagcgggtca
    60181 aacgatcctt atgccttacg tcgccaagca tacggtgtcg ttcggatcat cgaatcgaaa
    60241 aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta gtgatgttat cagtaaagat
    60301 acggatcgtt tcggcattgg tttatcagaa gggcaacaac aggtgatcga ctttatcaaa
    60361 ggccgtctgc gccaattatt gacaacgaag aatattcgtc atgatgtcat cgaagctgtt
    60421 ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag cagcacaatt gttcaagcag
    60481 catcttgcag atgaagactt caaaccatcg atggaagctt tgacacgtgt cgtgaactta
    60541 gcgaaaaaag cggagcttga aaaacaatca gaagtagacc ctgaactgtt cgaaaatgaa
    60601 gcggagaaag aactgcacaa agcagtagag gcaatgaaaa aaagctttgc tgaccaaacg
    60661 atcaacgaaa attatgatca gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat
    60721 cagacgatgg tcatggcaga agaaccagca gtacgtaaga accgattaac gcaattaagt
    60781 caaattgctg ccatggcttt gtcgctagca agtttagatc aaatcatcac aaaataattt
    60841 ctaatatgta aataaaccag agaataggca caaaa
//
LOCUS       DANKAZ010000004        59982 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000004 DANKAZ010000000
VERSION     DANKAZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            384..572
                     /locus_tag="PWY67_000229"
     CDS             384..572
                     /locus_tag="PWY67_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL2056542.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            650..1663
                     /locus_tag="PWY67_000230"
     CDS             650..1663
                     /locus_tag="PWY67_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2056543.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1730..2764)
                     /locus_tag="PWY67_000231"
     CDS             complement(1730..2764)
                     /locus_tag="PWY67_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2056544.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2879..5104
                     /locus_tag="PWY67_000232"
     CDS             2879..5104
                     /locus_tag="PWY67_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2056545.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5122..7005
                     /locus_tag="PWY67_000233"
     CDS             5122..7005
                     /locus_tag="PWY67_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL2056546.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6986..7960
                     /locus_tag="PWY67_000234"
     CDS             6986..7960
                     /locus_tag="PWY67_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL2056547.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            8619..9641
                     /gene="aroF"
                     /locus_tag="PWY67_000235"
     CDS             8619..9641
                     /gene="aroF"
                     /locus_tag="PWY67_000235"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL2056548.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            9674..10738
                     /gene="aroB"
                     /locus_tag="PWY67_000236"
     CDS             9674..10738
                     /gene="aroB"
                     /locus_tag="PWY67_000236"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL2056549.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            10740..11906
                     /gene="aroC"
                     /locus_tag="PWY67_000237"
     CDS             10740..11906
                     /gene="aroC"
                     /locus_tag="PWY67_000237"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL2056550.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            11923..13014
                     /locus_tag="PWY67_000238"
     CDS             11923..13014
                     /locus_tag="PWY67_000238"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL2056551.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            13027..14322
                     /gene="aroA"
                     /locus_tag="PWY67_000239"
     CDS             13027..14322
                     /gene="aroA"
                     /locus_tag="PWY67_000239"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL2056552.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            14315..14824
                     /locus_tag="PWY67_000240"
     CDS             14315..14824
                     /locus_tag="PWY67_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL2056553.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            14821..15657
                     /gene="pheA"
                     /locus_tag="PWY67_000241"
     CDS             14821..15657
                     /gene="pheA"
                     /locus_tag="PWY67_000241"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL2056554.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(15720..17456)
                     /locus_tag="PWY67_000242"
     CDS             complement(15720..17456)
                     /locus_tag="PWY67_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2056555.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(17453..18382)
                     /locus_tag="PWY67_000243"
     CDS             complement(17453..18382)
                     /locus_tag="PWY67_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2056556.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            complement(18382..18960)
                     /locus_tag="PWY67_000244"
     CDS             complement(18382..18960)
                     /locus_tag="PWY67_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2056557.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            19233..20519
                     /locus_tag="PWY67_000245"
     CDS             19233..20519
                     /locus_tag="PWY67_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2056558.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            20656..21525
                     /locus_tag="PWY67_000246"
     CDS             20656..21525
                     /locus_tag="PWY67_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2056559.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            21522..22409
                     /locus_tag="PWY67_000247"
     CDS             21522..22409
                     /locus_tag="PWY67_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2056560.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            22576..23259
                     /locus_tag="PWY67_000248"
     CDS             22576..23259
                     /locus_tag="PWY67_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2056561.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            23256..24029
                     /locus_tag="PWY67_000249"
     CDS             23256..24029
                     /locus_tag="PWY67_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2056562.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            24026..24424
                     /locus_tag="PWY67_000250"
     CDS             24026..24424
                     /locus_tag="PWY67_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL2056563.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            24532..25233
                     /locus_tag="PWY67_000251"
     CDS             24532..25233
                     /locus_tag="PWY67_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056564.1"
                     /translation="MKFYSKHQTFFSKYAILKDTLIERSTFLYVQLQENKTLLHFSPE
                     FHLEGLTLGEIYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMID
                     TNDGKIHLTAAELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLK
                     KYPGLLHTSIQFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFT
                     RNRNVLHQVAMEKAN"
     gene            25292..25732
                     /locus_tag="PWY67_000252"
     CDS             25292..25732
                     /locus_tag="PWY67_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056565.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            25744..26412
                     /locus_tag="PWY67_000253"
     CDS             25744..26412
                     /locus_tag="PWY67_000253"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2056566.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            26476..27099
                     /locus_tag="PWY67_000254"
     CDS             26476..27099
                     /locus_tag="PWY67_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL2056567.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            27155..27541
                     /locus_tag="PWY67_000255"
     CDS             27155..27541
                     /locus_tag="PWY67_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL2056568.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            27754..29145
                     /locus_tag="PWY67_000256"
     CDS             27754..29145
                     /locus_tag="PWY67_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL2056569.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            29756..30124
                     /locus_tag="PWY67_000257"
     CDS             29756..30124
                     /locus_tag="PWY67_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL2056570.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            30304..31245
                     /locus_tag="PWY67_000258"
     CDS             30304..31245
                     /locus_tag="PWY67_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL2056571.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            31314..32045
                     /locus_tag="PWY67_000259"
     CDS             31314..32045
                     /locus_tag="PWY67_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL2056572.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            32189..34369
                     /locus_tag="PWY67_000260"
     CDS             32189..34369
                     /locus_tag="PWY67_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL2056573.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            34389..34871
                     /locus_tag="PWY67_000261"
     CDS             34389..34871
                     /locus_tag="PWY67_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL2056574.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            34887..35648
                     /locus_tag="PWY67_000262"
     CDS             34887..35648
                     /locus_tag="PWY67_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2056575.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            35779..37113
                     /locus_tag="PWY67_000263"
     CDS             35779..37113
                     /locus_tag="PWY67_000263"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL2056576.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            37420..37905
                     /locus_tag="PWY67_000264"
     CDS             37420..37905
                     /locus_tag="PWY67_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL2056577.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            37898..38581
                     /locus_tag="PWY67_000265"
     CDS             37898..38581
                     /locus_tag="PWY67_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL2056578.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            38707..41352
                     /gene="polA"
                     /locus_tag="PWY67_000266"
     CDS             38707..41352
                     /gene="polA"
                     /locus_tag="PWY67_000266"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL2056579.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            41397..42233
                     /gene="mutM"
                     /locus_tag="PWY67_000267"
     CDS             41397..42233
                     /gene="mutM"
                     /locus_tag="PWY67_000267"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL2056580.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            42197..42826
                     /locus_tag="PWY67_000268"
     CDS             42197..42826
                     /locus_tag="PWY67_000268"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL2056581.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            42878..43210
                     /locus_tag="PWY67_000269"
     CDS             42878..43210
                     /locus_tag="PWY67_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2056582.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            43522..44013
                     /gene="nrdR"
                     /locus_tag="PWY67_000270"
     CDS             43522..44013
                     /gene="nrdR"
                     /locus_tag="PWY67_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL2056583.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            44036..45409
                     /locus_tag="PWY67_000271"
     CDS             44036..45409
                     /locus_tag="PWY67_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2056584.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            45409..46335
                     /gene="dnaI"
                     /locus_tag="PWY67_000272"
     CDS             45409..46335
                     /gene="dnaI"
                     /locus_tag="PWY67_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL2056585.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            46464..46904
                     /locus_tag="PWY67_000273"
     CDS             46464..46904
                     /locus_tag="PWY67_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2056586.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            47048..47749
                     /locus_tag="PWY67_000274"
     CDS             47048..47749
                     /locus_tag="PWY67_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL2056587.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            47746..48867
                     /locus_tag="PWY67_000275"
     CDS             47746..48867
                     /locus_tag="PWY67_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL2056588.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            48882..50114
                     /gene="pepT"
                     /locus_tag="PWY67_000276"
     CDS             48882..50114
                     /gene="pepT"
                     /locus_tag="PWY67_000276"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL2056589.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            50246..51154
                     /locus_tag="PWY67_000277"
     CDS             50246..51154
                     /locus_tag="PWY67_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL2056590.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            51189..51461
                     /locus_tag="PWY67_000278"
     CDS             51189..51461
                     /locus_tag="PWY67_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL2056591.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            51472..52518
                     /locus_tag="PWY67_000279"
     CDS             51472..52518
                     /locus_tag="PWY67_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL2056592.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            52769..55378
                     /gene="clpB"
                     /locus_tag="PWY67_000280"
     CDS             52769..55378
                     /gene="clpB"
                     /locus_tag="PWY67_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL2056593.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(55529..56215)
                     /locus_tag="PWY67_000281"
     CDS             complement(55529..56215)
                     /locus_tag="PWY67_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056594.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(56193..56687)
                     /locus_tag="PWY67_000282"
     CDS             complement(56193..56687)
                     /locus_tag="PWY67_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2056595.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(56707..57927)
                     /locus_tag="PWY67_000283"
     CDS             complement(56707..57927)
                     /locus_tag="PWY67_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2056596.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            58074..59909
                     /gene="lepA"
                     /locus_tag="PWY67_000284"
     CDS             58074..59909
                     /gene="lepA"
                     /locus_tag="PWY67_000284"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL2056597.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        19627 a        10647 c        12953 g        16755 t
ORIGIN      
        1 tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg tgcaaaatca
       61 aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa
      121 ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca
      181 tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga cgacgtagca
      241 gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg
      301 tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt
      361 tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg
      421 aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt
      481 caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag
      541 cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata
      601 acactatggt ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa
      661 aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt
      721 tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa
      781 tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa
      841 aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa
      901 aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg
      961 agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt
     1021 ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt
     1081 gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga
     1141 acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat
     1201 acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag
     1261 tctgaaagga accatttctt attggggacc tgttgatttg aagctaacag cacagcagca
     1321 ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac
     1381 aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa
     1441 ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca
     1501 agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga
     1561 tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa
     1621 ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc
     1681 gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt
     1741 ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg
     1801 gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg
     1861 atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat
     1921 tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc
     1981 gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt
     2041 cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat
     2101 gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc
     2161 tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc
     2221 cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca
     2281 tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga
     2341 cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa
     2401 caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat
     2461 tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca
     2521 aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa
     2581 tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag
     2641 ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca
     2701 acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa
     2761 ccatttttac acactcctca aaaaaataaa aaatttttat tgacaaaatt gtttagtaaa
     2821 cagtatacag gaattataaa aaaaagtaaa cggtttccgt tttgttggga gtgaaatgat
     2881 ggatattacg catagaaaaa cagataaaaa tttcatctta tttgatgaaa agagccgagt
     2941 cttccattta cgaaatacat ttctttctta tctgataaag atagaagaaa gcaatgtatt
     3001 ggcgcatatt tattttggca aaccagtcaa gcagtacaaa gacaataaaa attatccccg
     3061 aagagatcgt ggattttcag gaaatgttcc cttaaatcct gaccggtctc tgtcgaaaga
     3121 tacactaccg caagaatact cttctcatgg cagcatggac tttcgaacgc ccgcatcgat
     3181 cattcagcgc aagaatggat cagacttact ggatctgaga tatgacagcc attatattac
     3241 agatgggaag ccagagatcg aaggcttgcc tcaaacttat gtgatggaca agtcagaggc
     3301 acaaacgttg gtgatttcac ttaaggatag agaaacagcg atttatttcg atttgttcta
     3361 taccatattt actgatagag cagtgatcac acgatctgta aagataagaa atgaaaccgg
     3421 agaaacaatc aaactagaaa aagcggctag tttccagcta gactttgccc atactaggcg
     3481 atttgatgaa gtcattgctt tgccaggagc acatgtcaat gaaagacaga tttcacggca
     3541 atctgtttta tctggaacaa aagtatttga aagccgtcga ggaacgagca gtcaccatat
     3601 gaataacttt attgctttag ttcatcatca tacgacagaa aatactggag aagcgatcgg
     3661 acttcaattt gtttattccg gcaaccattc tttcgaactt gaaaaagatc agatcaatca
     3721 attgagagtc gtcggtggga tcaacagcca tcgtttttcg tgggaattga atgcaggcca
     3781 gtcttttcaa acacctgaaa tgatcctttc ttactcaagt cagggattga acaaaatgag
     3841 tcagatccac catgagttgc tacgtgaaag aatcgcaaga ggtcgtcatc aatttgctga
     3901 acggccgatt ctggtcaata actgggaagc cacttacttt gacttcaata gtgaaaaaat
     3961 caaggcgatc attgatgaag caaaggagct aggcatcgaa atgttcgtat tagatgatgg
     4021 ctggtttgga aaaagagatg cggacaattc ttcattagga gactggttcg agtatgaagg
     4081 caagttgaca aatggattac gtgaaatagc agattatgct catagcaaag gattgaaatt
     4141 tggtttatgg tttgaacctg aaatgatctc ggttgattct gaactgtatc gaacacatcc
     4201 tgattttctt atgcaggagc caggtagaat gccttctgct tccagatcac aacatgtact
     4261 ggattttaca aggttagatg ttcggcagac gattgagaaa caaatgagaa agattttaga
     4321 tacgattcct cttgactaca tcaaatggga tatgaaccgt tcgctttcag atgtctattc
     4381 gattaccctt gacccacaga gacaaggaga agtagcgcat cgttatatgc tcgggcttta
     4441 tgaactactt gaacatttga caactgatta tccggaaatc ttatgggaag gctgttctgg
     4501 tggaggtgga cggtttgatg ccgggtttat ttattatatg ccgcagtcat ggacaagtga
     4561 taacacggat gcagtggagc gtatgaaaat ccaatatgga acaagccttg cttatccgat
     4621 ttcgtccatc acagctcatg tatcagctgt accaaatcat cagaccgaca gatctacttc
     4681 gttgaaaaca agaggagaaa cagctatgag cgctgttttt ggttatgaac tggatttgac
     4741 aaagctgtct cctgaagaaa aaaagcaagt aaaagaccaa attatatcct atcaaacgat
     4801 tcgtccggtg atccagtatg gccattatta tcgtttggcg agtccttttg aagaaaatat
     4861 tgccgcatgg atgtttgtgt ctcctaaaca agatgaagct attgtgtttt tagggaggat
     4921 actagcttca gctcaaccag cgttccatga agtatatctg atggggttag atgatgaggc
     4981 actttatcag gaacagacct cgaagcggat attttcgggg gccgaattga tgacagttgg
     5041 actttacttc cccgattttc aaggtgattt ccaaacagaa ctgcttcatt tcaaaaagtt
     5101 atgagagaga aggaaaaaag tatgaaagca aatataaaat ggttagatga tccacaagtt
     5161 tttagagtga accaatcaaa tgcacatagc gatcatgttt gttatgcaac aagggaagaa
     5221 gtagaaacca catcctccct taaacagtct ttaaatggat catggcgttt ttgttattcg
     5281 ccaaatcctt cggtacgacc aaaaacattt tataaaaaag attttgatgc ctgttctttt
     5341 gatgaaatca ctgttcctag tcatattgaa ttagcaaatt atgacaagat ccattacatc
     5401 aatacgatgt atccgtggga gggacatgta tttcgccgtc cccctttcac gacaagccct
     5461 gagaaagagg cggaagggat cttttcagct gctaaagaca atccggttgg ttcgtatttg
     5521 acgacttttc gattagaaga aggtctaaag ggaaaacaaa tctcgattca ttttgctggt
     5581 gtagaacaag caatgtttat ttggttgaat ggtcatttta tcgggtatgc cgaagattcg
     5641 ttcactccgt ctgaatttga tttaacacct tatttggaag aagaaaatat tctagctgtg
     5701 gaggtttaca aaagaagtac agctgcattc ttggaagatc aggacttttt ccgatttttt
     5761 gggatcttca gagatgtctt cttagttgct cgaggacaaa accatttaga agatctttgg
     5821 atcaaacctc gcttagatga caaatgtcaa aatggcaaac ttgaagtaga tttgtctttt
     5881 gagcaagtag ccaaaaacag tcagctgaat gtagcgatac tagatcctga tgggaaggaa
     5941 atctatcaac agcaatatcc gttaaaagag caaatgacga ttcgcatcga tggatttgaa
     6001 aatgtggcac tgtgggggca ttttcagccc aatttatata agcttgaatt ggagacgata
     6061 gacgaagaaa aaagagtaac ggagtatatt gactattcct tcggttttcg taaaatcgaa
     6121 atcaaagata aaatcatcta cttcaatgga aaacggatga ttatcaacgg ggtaaatcga
     6181 catgagtggc atccgaaaaa aggacgcgcc atcactatag aagatatgga aaaagatttg
     6241 gctatcataa aaaatgcaaa gatcaacggc gtacggacat ctcattaccc caaccagatc
     6301 acttggtatt atttatgcga ccaagcagga atccatgtga tggcggaaac gaacctcgaa
     6361 tcccatggct catggcaaaa aatgggcgca tttgagcctt cttacaacgt accaggtgca
     6421 gtacctcagt ggcaggcggc tgttcttgat cgagcaaaaa cgaatttcga aacattaaaa
     6481 aaccatccaa gtattctttt ttggtctctt ggaaatgaat cttatgcgga agaaaacatt
     6541 cgacaaatga acgattactt caaaaaagta gaccccgatc gacttgtcca ttatgaaggg
     6601 aattttccaa atccagcatt cgaagattcg atttcagatg taaaaagctg catgtatgcg
     6661 ccaccagaag agatcattga ctaccttgaa aataaagcgg aaaaaccgtt catcttatgt
     6721 gaatatatgc atgacatggg gaattcgtta ggtggaatgg attcttacat gacgttgctt
     6781 gatcgttatg aaatgtacca aggaggctat atatgggatt ttaaagatca ggcgatccaa
     6841 gtgactgatc caatcacagg cagagaagtg atgcgctatg gcggtgactt tgacgataaa
     6901 ccaagtgact atgaattctc agggaatggg atcgtttttg cagatggaca agaaaaaccc
     6961 gccatgcagg aggtaagata ttattatgaa aaatacagta aataaaagtc atatggatac
     7021 ggaaaaagtt gcaatcgtct tcggcgactg tacattaggt gtcaaatcgg ggaatacgca
     7081 ttatattttt tcttatacaa gaggcggact ggaatcgctc aataaaaacg gaaaagaatg
     7141 gctataccgg gaaacacttc cagttttttg gcgagcaacg accgataatg acagaggtag
     7201 cggatttgga tttaaatccg gtatgtggtt aggagccgga ttgtatccaa aagtcaaaga
     7261 aatagaagta ctggtggaca ataaaaagat cgatttgccg attgctccag taaataatca
     7321 atattcaagt aatgaatacg ctgaaacggt tgaaatcatt tttacactgg aaatcaacac
     7381 agtaccaaaa acccaagtga caatttccta ccaaatcgct ggagagggta aaatgcttgt
     7441 caatatgcat tattatggac aaaaaggatt accggaatta cccgcattgg gtttaaggtt
     7501 cattatgcca acgaaagcaa aattttttga gtatgaaggc ctatcagggg aaacatatcc
     7561 ggatcggatg gctggtggtg tcgaaggtat ttataaagtg gcaggactcc ccgtttcccc
     7621 ataccttgtt ccccaagaat gcggcatgca tatggaaacg aaatgggtag aactaacaag
     7681 aaatcaaacg ttggataata cggataaaga tcaagaagat ttttcgttgc gatttgaaca
     7741 gcaggatacg ccatttgctt tttccgcttt accattcaaa gcagaagaac tggaacaggc
     7801 aacacatata gaagaactac cgccagaaag acgaacggtt cttacgattt ttgcaaaagt
     7861 aagaggagtc gggggtatca atagctgggg agcggatgtt gagtcacctt accgaatcag
     7921 cagcgaagag gaccatgaaa tcggctttat tattcagtag caatctttag attattattt
     7981 gaaaaacgga gtttacaaga caaagctgct ttattctata tgtcatgaac aagagagtaa
     8041 aaaagttctc agatactttt gttcatgact attttcttca ataaaaccaa ttgttatttt
     8101 tctgaaaaac aaagttaact atcagaaaat taaaaaaagt ttcataaaaa tgattgacag
     8161 tcgatttttt tatgtctata ctaggtacaa ttcttacatc agtttggagc gattttttat
     8221 gaaatctaga aaacttacaa aactgaatac gtattacttt agctatttta gataggtttg
     8281 tattcatgac ttcgtgggta caaacaaacc aggtttgtat ctatgatggc taaagatttt
     8341 taaacacgac ttacagccac gtagagaaat tcatctatag tggctatttt atatttttgc
     8401 agcgcttttt aaggctcgtt gaagatgttc actgtagagg caatttccta cagggacatc
     8461 tttttttgtt gattatctgg gtcataagta ggaaagaagg agcgaaaatg agaacaacag
     8521 gacacggtca tttagtagga attaatgtaa aaccaccttg aaaaagtaga gaaaatcagc
     8581 caagatagat aagtaaacaa aagaaagcgg gaaaaacaat gatagtaatt atgaaaaaag
     8641 aagcaacgga agaacaaata aaaaaagtcg tcgatcatat cgaaaaagaa ggattcaaag
     8701 tctcaatcga tcaaggaaca gaacgagtag tgattggact aaaaggtgat acgcgaagtt
     8761 tacaagaagg agcatttaca cgctatgaag gagtagaaaa tgctgtgcgt attttaaaca
     8821 catacaaatt gacttctcga gaattccatc ctggcaatac agtcgtggat gtagacggag
     8881 taaaaattgg tgacggcagt ttcgtgacga tggctggtcc ttgttctatc gaaggattag
     8941 accaaatcag agaatgtgcg cgaatggcca aagccggggg cgcaaaaatt ctgcgaggtg
     9001 gtgccttcaa accacgtact tctccatatg ctttccaagg tttagaagaa gaaggattga
     9061 aatacatccg tcaagcggct gatgaatttg gaatgaaagt gattactgaa gtgatggatg
     9121 aagggcatat cgatatggtg gctgagtaca gcgatatcct acagattggc gcacgtaata
     9181 tgcaaaattt taaactgcta tctgctgtcg gtaaaacagg taaacctgtt ggattgaaac
     9241 gtggaatttc cggtacgatc aatgaatggc ttaatgctgc agaatacatt gcagtcgaag
     9301 acaaatctcc tgtcattttt atcgaacgtg ggattcggac ttacgaaaca gcgacacgta
     9361 atacgtttga tttaagtgct gtcccattga tgaaaaaatt aactcatttc cctgtgattg
     9421 ttgatccaag tcatggtaca ggaatttggg aacttgtacc accaatggct cgtgcgggtg
     9481 tggcaagcgg agcagatggc atgatcgtag agatccatcc tgatcctgca aatgcttggt
     9541 cagacggtcc ccaatcgttg aatgaaaaga cttactcacg catgatgaaa gaagttgcga
     9601 tcatgaaaga agcaatggaa aagattcaga cactcgatta attccagctt tttaaaagaa
     9661 agaagtgaaa cgaatgctta ctgtacatgt tgataaccaa aaatacgata ttcatattga
     9721 aaaaggcgca ctagaacagg ctggatcatg gcttcaaacg atttggagac ctcaaaaagt
     9781 agctgtgatc actgatacaa acgttgctcc actatatgca gaaaaaatca tgttccaatt
     9841 gagacaagct aattttgaac cagtcctttc aatcgtaccg gcgggtgaga ccagtaaatc
     9901 tttagaacaa gcagctgagt tatacgattt tttagcagat aatgatttta cccgcagcga
     9961 tgggatactt gcacttggtg gaggaatggt gggtgatcta gcagcattcg tggcctcgac
    10021 gttcatgcgt gggatccatt tcgttcaaat tccgactacg ctacttgcac aagtagattc
    10081 tagtattggc gggaagacag cagtcaatac aaacaaagcg aaaaaccttg tcggaacatt
    10141 tagccagcca gctggtgtat tgattgatcc tgaggttcta aaaactttgc cggtacgtcg
    10201 tttacgagaa ggaatcgctg aaatcatcaa atcagcagct atagcagatg caacgctttg
    10261 gaaacagctg aacgagctga aagacgaaaa agatcttctg gaacatgctg ttccaatcat
    10321 tactgctacg ttggaaatca aacgaaaagt agtggaagaa gatgtctttg accaaggaaa
    10381 tcgacttatt ttaaattttg gtcatacaat tggccatgca attgaaaata cagcaggata
    10441 tggggtagtc agtcatggag aaggagtagc tattggaatg gtagccatca cttctcatgc
    10501 agaaaaaatc ggcttgtctc caactggatc gacagctgct ttagaaagga tacttcaaaa
    10561 attccatctg ccgattacgt acgatctaag tcatccagat gaattattcc acgcgataac
    10621 ccatgataag aaagcaagag gaagccaatt gaatatcatc ttgctagaaa agattgggca
    10681 agcaaaaatc gttcctgttc ccacagagac attcaaagat tacttaggag gactgtaaga
    10741 tgcgttatat gactgcggga gaatcccatg gaccagaatt aacagccatt attgaaggac
    10801 taccagcagg attacctctg tcagaagaag aaatcaatca cgaactcgct cgcagacaag
    10861 ctggttacgg cagaggagga aggatgaaga tcgaaaaaga tcgtgttcgg atcacgtctg
    10921 gcatccgtca cggcaaaaca attggttcac ctgtcacttt agtggtagaa aataaagatt
    10981 ggaaaaattg gacaactgtc atgtctgtag cagaagtgcc tgaaaaagac caatcactgc
    11041 ggcgagttgc acgtccgcgt ccaggacacg cggatttggt aggcggtatg aaataccatc
    11101 atcgagatct tcggaatgtg cttgaacggt cctctgcaag agagacgacc atgcgtgtag
    11161 caatcggcgc aattgccaag aaaattctgg aagaacttga catcatcgta gcaggacacg
    11221 tgatttgtct tggtggaatc caagcagaaa tacctggaaa tatctcgatt caagaaatca
    11281 aagaaatttc tgaagcgtca gaagtacgag ttgttgatcc agcagttgag caaagaataa
    11341 aagatttgat cgatcagaca aagaaaaatg gagatacgat cggaggaatt gtagaagtgc
    11401 tcgttggagg cgttccagct ggattaggaa gctacgtaca atgggacacg aagctagatg
    11461 ccaaaatcgc acaagctgtg gttagtatca atgcctttaa aggcgtagaa tttggggtcg
    11521 gattcacttc tggttcatta ccaggctcag aagtgatgga tgagatccta tgggaagaag
    11581 aaagaggcta tttccgacgt accaataatc taggcggctt cgaaggcgga atgaccaatg
    11641 gcgaaccact tgtcattcgc ggggtcatga aacccattcc tacattgtac aagccacttc
    11701 aaagtgttga tatcgataca aaagaacctt acaaagcaag tgtagaacgt tcggatagta
    11761 cagcagttcc tgcagctagc gtcgttgcgg aaaatgttgt agccactgtg gtagcgacag
    11821 agctattgtc aaaattccca agtgattcgc ttgttgagtt gaaagctgca gtgaaggagt
    11881 accgtgacta cttgaaacat tactaaaaag ggggcaaata agatggcaca acatgttttt
    11941 atcgtggggc ttggactgat tggtgcttct atggcacttt gtattcgtca gaccaatcca
    12001 gaactgatag tagttggttg ggatagtcag tcaaaaaccc gtgagcaagc agtagaacag
    12061 cagatcgttg attacgttgc aagtgatttt gaaacaggtg cagaacaagc agacgttatc
    12121 ttattagccg ttcccgtccg tacaacctta gcttatcttg atgtccttga aagaatcaac
    12181 ttgtctgatc acgtcatcat tacggatgtc agcagtacga agcagcaagt cgtgacatcc
    12241 gctgagcaga aaaatcttcg gtttgtcggt ggacatccaa tggctggttc tcataaatca
    12301 ggtatacagg cagcagatgc taaactattt gaaaatgcct attacatctt tacacctttg
    12361 gaaaagacga aaaaagatgt tgaaaaactg caagaactat tttgcggaac caaagcaaaa
    12421 tatgtaactc ttacagcaag agaacatgac cgtataacag gaatgctaag tcattttcca
    12481 catattctgg cagctggctt agtcaatcag gcagaacaat tcaatcaaga gtatccgcga
    12541 gccaaacaac tggcagcagg aggctttcga gatatcacta ggatcgcttc ctcagatcct
    12601 gtcatgtgga cagatatttt gttgagcaat aagcagatat tattggaacg attggcagat
    12661 tggcaacaag aaatgacaca aatagcagaa tggatcatga ctgagaacca atcggaaatc
    12721 ttttcttttt tcaatcgggc aaaagaaagt cgagaccagt tgcctgtcca taagcaaggc
    12781 gcgattcctg ccttttttga tttatttatc gatgtgccag atgaacctgg cgttattgca
    12841 gaagtaactg gactgatcgg aaaagcggga gtttctttga ttaatttgaa gatccaagag
    12901 accagggaag atattttagg aatactgcaa atcagtttta aaaaccaaca ggatttgtta
    12961 caagcaaaaa gaatcattat gtctaatacc agttatcatt gctggatcaa ataggaggct
    13021 ggataaatgc aattattaca acaaatacat ggattaagag ggactgttag gataccagca
    13081 gataaatcaa tttcccatcg cagcatcatg tttggagcaa ttgctgaagg aacgacgact
    13141 atacaaaatt ttttgcgcgc agaagattgt ctgagtactt tacatgcctt ccaacaatta
    13201 ggtgtcgaga tcgaagaaga ggaagaggtg atcaagattc atggtcgcgg tagccactca
    13261 tttgtccaac caaccgaatc aattgacatg ggaaactccg gtacgaccat acgtttattg
    13321 atgggtattt tggctggaca gccttttacg actactctgt tcggggatgc ttcgttgtct
    13381 aaacgtccaa tggggcgagt gatggaacct ttacgcgaga tgggcgctga cttgcaagga
    13441 gaagaaaatg accagtatct accaatcact gtgacaggaa cccgctcttt atcacctatc
    13501 cgatacaata tgcctgtagc cagtgcgcaa gtcaaatctg ctttgctgtt tgcggcatta
    13561 caagcagaag gcacatccgt aatcgttgaa aaagaacgtt cccgtaacca tacggaagaa
    13621 atgattcgtc aatttggcgg aaggatcaca gtggaagata aaacgatcat agtgaccgga
    13681 ccgcaaaaat taactggtca acagataact gttccaggtg atatttcatc agctgctttc
    13741 tttctagcag caggacttct tgttccggaa agccaactgt tgttaaaaaa tgtcggggtc
    13801 aatccaacaa gaactggtat cttagatgtg ttggaggaga tgggcgcagc gattacccag
    13861 acgaatcaca atgaacagaa ccaatcggct gatttaagtg tcaagacgag tcatttgaag
    13921 aaagcaacga ttggtggaga aatcattccg cgtctgattg atgaattgcc cattctagct
    13981 ttagtggcta ctcaagcaga aggaatcacg atcatcaaag acgcagaaga attaaaagta
    14041 aaagaaacca acagaattga tgcggtggca gaagaattac agaaaatggg agcaaagata
    14101 aaagcgacag cagacggtct gatcatccat gggccgacac ctctgcatgg cgcaaaagtg
    14161 tccagcagag gcgatcatcg aatcggtatg atgctgcaag tagctgcttt gatagctgat
    14221 ggaccttgcg aactagaagg agctgaagct gtttcaatct cttatccggc attttttgac
    14281 gatttagcag aacttgtgtc aggaggagaa gctcatggct agcattttgc tgattggttt
    14341 catgggcgct ggaaaaacaa caatcggtaa agggttagct caatgtttac aaaaagagta
    14401 tgtagattta gatacaaaga ttgaggaaca tatccaattg tcgattgccg aatattttca
    14461 ctattacggt gaaaaaagct ttcgaaaagt ggagtcagat attttaagaa agctgtcaaa
    14521 tgaagacaaa attatcgcga ctggtggagg gattgtccaa agtgcagaaa atcggcgttt
    14581 tttaaaaaca cagccgattg ttctttactt agaggcagaa gcggactgtc tagttgatcg
    14641 tatccaacaa gatgaaacgt ctattcgacc tttagctctt ggaaaaacaa gagaagaaat
    14701 aaaagcatta ttggcacaac gactttcttg gtatgaagaa agtgcgacac atcgaatcaa
    14761 cacgaccaat caaacaccag aaaagatcat cgataccatt atagaaagga tcaaaagcat
    14821 atgaacgtca gctatctagg gccagaaagt tcattcactt atcaggcggc ttgtcagcta
    14881 tttcctacag aacaattgac tgcgtatgct tccatccctg tttgtttgcg cgctttgttt
    14941 cgcaaacaag tcgatctggc tgtcgttcct gtagagaatt cattggaagg agctgttcat
    15001 cacacaatcg atcttctttc caaacatcca gaagtcgaag ttaaaagtga aatcgttctt
    15061 cctatcaagc agcagctttt aggcaatcca gcaacaaaga ttacaaaaat cctttcacat
    15121 ccacaagcac tagcacaatc acaacagttt ttagaaacgc attatcctaa tgttccttta
    15181 gtagcaacag aatcaacaac tgctgcagca atgtatgtag cagaacatcc gaaagaagac
    15241 gcagcagcga ttgcttcatt agaaacagca cagcatgttg gattggagat cttagctgag
    15301 aatatccaag ataatgagtt gaaccagaca cgtttttgga tcgtcggtga ccgaaagatg
    15361 accagccagc agtcagctcc agtaaaaatg agtgtcatct tgacactacc tgctaatcgg
    15421 ccagggatgc ttcataaaat gctggcagct tttggctggc gggaaatcaa cttaagtaag
    15481 attgaatccc gaccattgaa aactagcttg ggagaatatt ttttcgtgat cgatttgttg
    15541 ttggaccgtc caatgacatt agtagagaat gcgctccaag aaatcaagat gctcggagga
    15601 gaaagtcaaa tattgggctg ttatccagtg ctgacagtag aagagacgcg tagatgaaag
    15661 tcaagaatac tgattgattt atttaactta aaataaaaaa atcagcgctt ggctattctt
    15721 tatttttgat attctgattg atttttagga agaagcggta gacaaatcgt tacagtcgtt
    15781 cctgtcccaa tcactgattg gatcgttaag ccatatgcat cgccatacat taggcgtatc
    15841 cgctcatcta catggctgat gccgattcct gaaaaatgat catttttttg tttggtattt
    15901 tcagtatcga agccatcgcc gttatcaatc acttcgaata aaagaagctg attttttgtt
    15961 ttgccgtaca ccgtgatgaa tccttgcgtt ttattgtgaa aagcatgaag ataggcgttt
    16021 tcgataattg gttgaaggat catattcggc atagataatg actgcagttc atcaggaatc
    16081 attattttag ccgagattct ttcgccatat cgcatactca aaagatcgat gtaactttgt
    16141 acgcttttga tttcttcctt gactggaatc acttcttctt ttttcccaat cgtacttctt
    16201 agtaaagtaa tgaaactgtc gattccttga actgcttcat ctgttttttt ctgccaaatc
    16261 aaaaatttca agctagtcag cgtattataa ataaaatggg gctgtatctg tgcttgcata
    16321 gtttggattt caagcgaacg tttcttcttt tcgtcttgga tcgtctgttc ataatgatgc
    16381 tgagcattac ttagcaattg attgatcgta ttccctagca attggacttc atggcttcct
    16441 tgtacaggaa cagtagcttt taagccatct tcctctagcc gttttagcga atcaatcaag
    16501 cggtagatcg attttgtgtg tcgatccaaa tacataaata ccaatgagcc agcaaaagca
    16561 agtgtcacga tattatacaa aataatcgga aaaatcacgc ccatttggtt agataaagaa
    16621 tgaagatcga cttgactgag taatgtacaa tcctggcggt acagaggaag acgggtcaac
    16681 tgctgtccat ttgaaatcgt tgtttcacct actgttagtt ttttgaaaat cattgatcgg
    16741 ctcccaattg tattttcttc atttgatgcg atgatcgtct gttcttcgtt catgaaggag
    16801 atcttcgata tctcaggatt tactacagat tgaaaaagat tcgctagttg cttggatgag
    16861 atcgataaaa cagcatatcc gtaaacgtga ttcaatgtat ttttcagttt tttgacaaag
    16921 aagacgctgg gtgctttctt atcgcgaaga gtcaatccgt ggtgtgcaaa aagcatttgt
    16981 gtatcagcag cacgttcatt cactctctga gcaatcgcag attgaaaaaa agaatcggca
    17041 gatacggcag tgagcatgtc gtttccgaca aaagttcttc cattttctcc aaagatcaat
    17101 acatcgtaat cgatcattgg atggattttt gaaagagagt cattcatttt ttggattgtt
    17161 cttagttgct gaagctgttc catatgttgg gctggatcat tggaaaaata gcttttgaca
    17221 gctggattag aatcaaaagc ggctaatgta ttctcgattg tatcattcaa actttcaaat
    17281 tcagattgaa tcttttttag taccacatca ttcgattgat tgaacagctg gatataggca
    17341 tcccttgatc gatgataaat aaacaaagaa atgacgatgg cttgcagaaa aagaccgatg
    17401 aacaaaatga acagaagttt ggcaaagagt tcatgctctt tgattttttt tctcatttta
    17461 caaaagttcc tttacggtat tgagaaggtg tcattcctgt ctcttcttta aaaactctgg
    17521 agaaataacc cagttcggta tagccgactt tctcacagat ttccgtcaaa gtaagccgtg
    17581 tttccgtcaa aaagagcttg gctttactga tacggacttt ttttagatat tttgaaaagt
    17641 tgataccaaa tttttcagta aagatggctg agagatagct atatgaaaaa tgatattttt
    17701 cggataaaag ttgtaaattg atttctttct cataattttc ctggacgaac tcgtagatca
    17761 aagtcaagat atcgaaattg tctttttttg catttgcaga aaagtgggtg agtttctcaa
    17821 gtgtttcttt gacaagaata gaaaaatctt cgaaatactc agcatcggct aataaagaag
    17881 gaagggcgat cttgagcaaa gaaagttctt cattcccaac atgctcgtca gccaaagcgt
    17941 tgagtaatgc atagaaacga ttgattgcct catgtttcaa ataagctgga tcaataggct
    18001 gttccaatat cgtcgtgaag aaattctcaa gttctttgac agctaaagcg tagtctttat
    18061 gaacgatttt tgttaaataa ctacttgctt gctgctcgtg gttggttaat aacggaagaa
    18121 gttcatgctg ataagcaagg ttctttccct tatgatagaa acgttgtcct ttggtgcaat
    18181 ttctgatctc ttggaatatt tcattgatat cttggattgc aaaagaatca gaaacaacaa
    18241 aaaaatcacc tggaaaaact tcttgacgaa acttttgaac aaacttccta agtgtatctt
    18301 tggaatcagt attgattaca tagccggatt cttccgaaaa ggtagaaaca ggataaatag
    18361 taaacgaatc gtcgctatcc atcaatgaat gggtgatcga ctgtttttgt ttagagcctt
    18421 gttttgaact gaatggccgt ttttcggtat ataacagaaa aacaaggtgt gttcaaaaag
    18481 aagagggtca agcaatggac gagtttgtcc agcaatctgc tgtgaaagat tctcgcagat
    18541 ctgctcataa cgattggtga ttgggctttt gttgggcttt tttttgattt tttgtgaaac
    18601 ggcagttaag gaatttagaa aaaaatcagg actcaatcga ggctttaaaa gataatcagc
    18661 gacgccttct ttaaaagaat ctttaaccat atcaaattca tcatagctag agaggataag
    18721 gatctgtgtt tcaggttgtt gctgttttaa tagccgcgat aattcgattc catttaattc
    18781 aggcatcatt acatctgtga ccacgatatc gattgcatgt accaaagaaa aatcaagcgc
    18841 tttcatcgga ctggaaaaag tccctattaa ttgaaacccg tgctcagacc aattgatcaa
    18901 ctgtttcatt ccatctaaaa tcgtctgttc atcatcgata agaattacgc gtattggcat
    18961 ttttttaccc gccttaagaa tagtcttgta atttaaaaat acttatttct aggagaaaat
    19021 gcaaggatat ttttatttta aagaaacctc accatttcga tctttggtct agatgctaag
    19081 attattcctt ttgtcatagt tgatttgtat ttttttatta ttttaggaga aatttttgaa
    19141 gtatctgaat aataaagaaa ccgttttcaa attttggtat gatgaatgta gcaaacgtaa
    19201 acaactaatg gatggaaaag gagcggataa aaatgaacat cagaaagtta attgctggag
    19261 tcgtttgttt agcggcagca tcaggtttct taacaggatg cggaagtgct agtagtacag
    19321 cagacaaaga agaaaaagtc actgtctggg cgtgggatga gacattcaat atcaaagcag
    19381 tgaatgaagc aaaaaaagtt tatgagaacg aagaaacaga gatagaagtt gttacgatgt
    19441 ctcaggatga tatcgttcag aaactgaaca ccgctttagc gagcgggaat acagaaggat
    19501 tgcccaatat cgtcttgatt gaagattata ggatacaagg ctatttgact tcttatccag
    19561 atgcattcgc taatttgtct gctattgtaa aggaagatca ttttgcaccg tataaatttg
    19621 cggtaaacaa agtaggagat aaaatatacg gtgttccttt cgattcgggc gtaacagcaa
    19681 acttctatcg tacggatctg atggcagaag caggttatac agaagaggat atggagaatc
    19741 tgaagtggga cgagtatatt caagtagcgc gagatgtcaa agaaaagacg ggaaagaaaa
    19801 tcgctgaagt caatccttct gaccttggtc gagtaaggat gattatgcaa caggcaggag
    19861 aatggtatac atctgaagat ggggagacag tcacgatcca agacaatgca tctttaaagt
    19921 atggtcttga attgtttgcc actcttttga aagaagatct agtcgaacaa acctctgact
    19981 ggaatgctgg agtaactgcg atacaatcag gagcagtagc ttctagccca actggcgctt
    20041 ggtattcttc aacgatccaa ggagcagaaa accaatctgg aaaatggaag attgcaccaa
    20101 tacctgcact tccagaaaat ctgcaaaaag cgcaagcttc taaccttggc ggcgctggct
    20161 ggtatgtgat caaaggagta gctggagaag attcagcgaa agatttccta gaagcaacgt
    20221 ttgcttcgaa tgaagaactg atgggcactc ttgcgaaaga aatcggacta gtatctacga
    20281 tgcttagcgc gaatgaacaa gaagcttatc aagaagcttc tgaattttac agtggtcaaa
    20341 agatctttga cgatttttca acatggacat ctgaaatacc agaagtgaat tacggacatg
    20401 agacctatgc aattgaagca gtcgtagctg aagctcttca tcgaatcatc aatggagaag
    20461 agacagacaa ggtactagca gatacacaaa aacaagtaga agcacaattg gctaattgat
    20521 cggtggtaaa aaagtgaatt tcttcgcttt ttcatcagac aaacgaaaag acaaaaaaga
    20581 tcacgtctgc cttacagata ttgtctttct tgtcttttca tgtagataat caatgataga
    20641 attggaggga aaagaatgaa gcagaaagat ttattcaaaa acgggcatgc ttttttaatc
    20701 ttgccagtca ttttgatatc aatcatggtt tttattccga tggttttggc tttgatcacg
    20761 tcttttcaaa gtggtccccc tgtcaatatg acatttaacg ggttaggtaa ttatcagcgg
    20821 atgttgacag atgcaacttt taaaaaagct ttttttaata cattcctgta tttacttgta
    20881 caagtaccga tcatgttgtt tctagcactt tttatttcca accttttaaa tgataaaaag
    20941 ttgaaatata aagggttgtt tcgaaccgct atctttcttc catgtatcac ttcgatggtc
    21001 agttattctc tgatcatgaa aagtctattt tctcaatctg gccttgtaaa caacttgttg
    21061 ttgggcattg gtatgattga cacaccgatt ggctggttga ctcatccaat ttgggcgaga
    21121 gttctgatca ttctttcgat cacttggcgt tggactggat acaatatgat ttttttcctt
    21181 tcaggcatgc aaaatattga ttcggagatt tatgaagcag catctattga cggtgcttct
    21241 aaatggcagc agtttatcaa agtgacgatc ccaaatttaa aaccaatcat tttgtttact
    21301 tccattactt cgacgatcgg gacattgcag ctttttgatg aagtacaaaa tattactggc
    21361 gggggaccag caaatgcaac gaccacccta tctcaataca tctataattt gagttataaa
    21421 ttcacgccta attttggata tgcggcagca gtttcctttg ttattgtttc ctttattgtg
    21481 atattgtcga taatccaaac gaaagtagga ggagaaagtc gatgaaaaat atggtagaaa
    21541 aagaaacaat cgaacgcccc aacgtcaaaa aacaagcatt agaacttcgt tcgacaattg
    21601 ggtcagtatt aaagtataca gcattaagca tcctatcatt tatttccatt ttcccttttg
    21661 tctggatggt tttgggaatg acgaatacac ccgtggatat cacatcggga aaaataaaga
    21721 ttggcagtaa cttgatcatc aattttcaaa atttgttttc caatgagctg aattttactc
    21781 gttccctttg gaattcagcg atgatcgctt tggtgaccac tgtgtttgca ttgctgattt
    21841 cgtcaatggc aggatatgga tttgagattt atcaaagtaa acgaaaagac ctaattttca
    21901 atattctact gctatcgatg atggttcctt ttgcggcatt gatgatccct ttatatcgaa
    21961 tgttttcaca attgaacgga acggttttgg gaatcaacag tttcttggtt gtcatcctgc
    22021 catctgttag cactgctttt ttgatcttct tctttcgaca aaatgttaaa tcttttccaa
    22081 aagatctagt agaagccgct cgattagacg gtctaaaaga actaagtatt ttttttagag
    22141 tgtatatgcc gacggctaaa aatacctatg ccgcggctgc aatcatcaca ttcatgagta
    22201 gctggaacaa ttatttatgg cccttagtag ctttgcaatc tcctgataaa cggacggttc
    22261 cgcttgtttt atctgctatg ggtgcttcct atacaccaga ctatggaatg atcatggctg
    22321 gattagtcat cgctacttta ccaactgcca tcgtattttt tgttttgcaa aagcaattcg
    22381 ttcaaggaat gatcggctct gtaaaataaa aaaacacaat aagcgcgttc cttcaaagtc
    22441 atttttccaa gtaaaaagat acttttggga cgtgtgttat tttatgttga gaaaaattaa
    22501 atgataacgt tttaagtttg tgctcgaaaa ctctttcatt ttaaggtaaa cttaaagagt
    22561 aaaggaggag gatacatgct aaaaaatttt aaagaagtga ttgctgctat attgccgatg
    22621 actgtcctga ttgtcatttt aacgtttgtt tttgcaccgt tagaaggaga agacctcgct
    22681 tcatttctgg ttggtgcggc aatcatgatg atcggaatga cgctcttttt gtttggagca
    22741 gattattcta tgatggaagt cggagatcta gtagggaaat atatgatcaa aaagaaaagt
    22801 ttgaccgttt tggtcagttt gggatttgct atcgggatcg tgatcacgat tgctgaacct
    22861 tccgtgcaag ttttgggaca acaagtatat gatatatctg acggagagat tggcagagtg
    22921 ttgctgattg ggatcgtcag cgtcggcact ggagtatttc tagcctttgc gctgcttcgg
    22981 gtcgtattca agctatccta ttatcaattg atggctatag gctacatagg cgttttgatc
    23041 gcctcatttt tcaccagttc cgaatttatg ccgattgctt ttgattcggg gggagtgact
    23101 actggtccag taactgttcc atttatcttg gcccttgccg gagggatgac tagtatgatc
    23161 aaacaaaaga aaaatgaaaa tgacagtttt ggaatggtcg gtattgcttc gttagggccg
    23221 attcttgccg tgatgatttt gggggtgatc ttccaatgag tttctggcag caagtgatag
    23281 atggaatcga aatcgttacc atggaagtca ttttggcagt ggcacctatc gtaatcctat
    23341 ttgttttaat gaatatcatt agttttcgta tgcgcaaaaa gcgtttcttt gacattatga
    23401 aaggtttctt gataacgact attgggttga tccttttcct gcacggagtc aatatggctt
    23461 atgtaccggt aggacagcat ttaggcagtg ctattgcggg attgccttac aattatatac
    23521 tggttcccct tggcttttta atggggttct tagtagggtt tgctgagccg gcgattcatg
    23581 taatgatcaa acaggttgaa gaattgagcg aggggagaat ccgatcgaaa atcatgctga
    23641 tggtaatttc aattgggatc ggcgcagcag taggattgtc tatgtggcgt ttgctcgtcg
    23701 gattctctct ttattatttc ttgatcccag gatacgggct agcatttatt ttaggacgaa
    23761 aagtggacaa gatcttttta gcaatggctt ttgataacgg tggagtagca acgggcccga
    23821 tgtgttcgac atttatttta tcaatgagcg tggcaatcgc tgcacaaatt gaaggaagag
    23881 atccgatcat tgacgggttt ggtgtggttg caatgattgc attgacacca atattatcca
    23941 ctttagtact aggatatatt tataaacgaa aagatgaaaa agacagaatc gaaaaagaaa
    24001 gattgcaaaa attgggggaa agcaaatgaa agatcttgta gcattgaatc ttcacatgat
    24061 tgtgacgatc gttgacggag gaatgggcga agatgttatc gacttttcaa aagaagcagg
    24121 tgcagcaggc ggaacgatct tacatggaag aggttctggt gtacatgatt cagggaaatt
    24181 tttaggtttg gacatttcgc cagaaaaaga tattgtgttg acattagtac ccgatagttt
    24241 gacgaataaa gtaatggatg cgattggtca aggcatcaac atcgatattc caggtaatgg
    24301 tatttgtttt agtattgatg tagacaaagt cattggtatc actaagattc atcaatatcg
    24361 agaaattatc ggaatgcaag aacttttaga tgaaaataac gatagagaaa agtctgaatc
    24421 atagacaagc gtttgaagag gaaataaaca caaacaagaa ggcggatgag cgttatttcc
    24481 gcttttttgt ttgcattttt ctcgtgaata cttatgaaat agtaaatttt tatgaaattc
    24541 tactctaagc atcagacttt cttttcaaaa tatgctattt taaaagatac tctgattgag
    24601 cgatctacat ttctatatgt tcaattgcag gaaaataaaa cattgctgca tttttcacca
    24661 gagttccatc tagaggggct aacattagga gaaatctatc aagcagaagg gctgtcggct
    24721 gttttgcact tttttaaggt agaattggaa ttaccagtaa aagattatat cgagctttct
    24781 cttgaatctt gggatcgagc agtggttgaa ctgtttccag aaggtttaat gatcgataca
    24841 aacgatggga agatccatct gactgcagca gaactgcaaa aacaaatgcg ttatcaaata
    24901 gatgacgaaa attctttttc tatttttaga cgtcagcaaa agattctaaa aagcttttta
    24961 aaagtgatca gtaaaaaacg gaatctgatc aaaacgacac agctgttgaa aaaatatcca
    25021 ggtctgcttc acacgtctat tcaatttcct caattgatta cgatgatcca tcgttttata
    25081 cgtatgcaac agctaccatc taagaagctg tttcttccat tgacggacac atttcgagta
    25141 gtcaacagag aagataaaaa gaaagtgatt gtgattgact ttaccagaaa tcggaatgtc
    25201 cttcaccaag ttgcaatgga aaaggccaat taaggctttt tctttttgga tttttttgtc
    25261 ataataaata ttagatgatg gaggagataa tatgatgcaa acatggttag aaaggctaat
    25321 acgatttcag cagaactatc acaaacttat gcagggaaga tatgcgaaat ttgatcagtt
    25381 gaatcgcggt ctattgatcg ctgcgcttgt ctttagtttg ctaagtcgtt ggttgcccta
    25441 tagaatcgga caattgttgt tcatcgtctt tttcggatgg atgatatttc gtttcctatc
    25501 aaaaaaaatc taccccagac taaatgagaa tcaacgttat ttgaaatatc ttgatagagt
    25561 caaagaaaaa tatcatcaga taaaaaacaa aaagcaagtc aaaatggatc gaaaaagtta
    25621 tacttttttt gagtgcccga attgtcatca aaagcagcgt gcacctaaag gaaaaggacg
    25681 aatacgtgtg acatgcaaaa catgtggtat aaaattcgaa acgaatgttt aagggagaaa
    25741 agaatgaaat tagtattatt acgtcatggc gaaagcgaag caaattttga aaattactgg
    25801 acaggatggc tagatgtcgc cttaacagaa aaaggaaaag aacaagcagc agttgccggt
    25861 caaaaaatgc ttgaagccgg acttcaattc gatgccgttt atacctctgt tttgaaacgt
    25921 gcgattctta ccggacaagt cgcactagaa gaaatgggtc agttatggct tcctataata
    25981 aaaacatggc gtctaaatga acggcattat ggggatcttg tgggtaaaaa caaagataag
    26041 atgaaagaaa tatacggaaa agatcaagta aaaacatggc gcagaggata tacggaagtt
    26101 ccgccgttaa ccgcagaaaa tagatttgac cgcagatatg atcacttgga tccgcatttg
    26161 atcccatacg gagaaagtct ggaaatgaca gtgaatcgga tcattccctt atggcaagat
    26221 catctagcac caaaactaaa agatcggcaa gatttactag ttgtgggtca tggtaatagc
    26281 atccgagcac tgacaaaata tttagaagat gttcctgaag atcaaatgga tacgatcgat
    26341 atcccaaatg cacaaccaat ccagtataca ttagcagatg atttgacaat tctggacaaa
    26401 aaaatacttt aaagaacacc tttatctaaa agtgagagat agaaaaaaga agcaaaggaa
    26461 tggaacaaaa ggaacatgaa aaattatata tgggactttg atggtacatt gtttgatacg
    26521 tatcccgcaa tggtagatgg tgcatggcaa gcactaaaag attttggtat cagtatggac
    26581 aaaaaagaaa tctacttcaa aatgaaaaag tattctacta gttatttgat caatgaatca
    26641 aacttgaatg ccagagagtt taatgaatta tttcaccgat atgaaaaaga aagtacggaa
    26701 gtttcccgtc catttccaga aacgaaacaa gtactggaaa tgctaaaaga caacggcggt
    26761 cgtcatttca tcctgaccca ccgtttgaca gaatcaacat ggggcctgtt aaaagaacat
    26821 cgattggcac atctgattga ggaagtagta gggatcgatc aggactttcc tcgtaaacct
    26881 gatccagcat ctttaaatta tttgatcgat acttttcatt tagaaagaac agacacgatg
    26941 atgatcggcg atcgtcgttt agatatcgaa gcagggaaaa atgcaggagt cgctacttgt
    27001 ctttacgaca tcgatcattt tctgggtgag atcccggcag attatgtggt aggaaatctg
    27061 aatgaaatcc ttaccttgca gagcatcatt aaaaagtagt atcccctttc aaaggagata
    27121 tgttataatt ttcataaagg agtgataaaa ttatatggaa atcagaagac aaaaagaagt
    27181 tgtagaagca tttcattatg atatgagatt gccgaaccaa gaagtagaaa cagatttgaa
    27241 agtaggcttc tcgcctttaa aagaaacgat cgaaaactat ccgaaagaac atagcatcgt
    27301 aggagcacgc ttagagttcc gtttagtgtt tgatacatat gttttatctg gaagtgtgag
    27361 tcagatcaat catatcctca atcggaaaat tgaaggtcaa aatgatgtca cacaagaaga
    27421 agtggacgag ctagtagcac cgttattcga tatcgtacgc cgaatgtctt atgaggtgac
    27481 agagatcgcc ttagatcagc ctggggtaaa attgaatttt caatcagaaa aacaagaata
    27541 gacagtaaaa acatacctgc gatagattaa agctatcaca ggtatgtttt tttattacta
    27601 attgtaagca aaaagaatgt catttcgata aaaatcgtct aaaatataaa agtcactgaa
    27661 aaaaattttt tcaggaggat ggttgaagaa tcattctcgg gaacctttac ttccttcgat
    27721 ggacttgtca ttaataatat gaggaggaga aaaatgacaa cactcatcac agttttagtg
    27781 gttctagttt ttgcggcatt attatatgtg ttctatcgta tgcaaaagcg ccatacaaaa
    27841 ttttcaacac gcgtctttac agcattagga gttggtatcg tctttggcgg tctgcttcaa
    27901 ttgatctttg gtgtagacga caaagtcgtt actcaatcaa tggaatggat cgggattgta
    27961 ggtcaaggat atgtatcatt cttgcaaatg ctagttatgc ctttagtgtt tatttcgatc
    28021 attggtgcat tcacaaaaat gaaagtttct gaaaagctag ggaaaattag tgcgactgta
    28081 ttgacttctt tacttggaac aacagctatt gcagcattga tcggaattgg ttcaacaatg
    28141 ctgttcggac tccaaggagc taaattcaca caaggagctg cagaaacagc acgaatctct
    28201 gaactggcaa cacgtcagga aacagttgaa aacttatcga ttccacagca gattttatcc
    28261 tttatcccta aaaacgtttt tgctgattta gcaggtagtc gtgcaacaag tacgattgga
    28321 gtcgttattt tcgcggcatt tgtcggaatc gcttatctag gcgtgaaaag aaaagcgcca
    28381 aaagaagcag cattcttttc agatttagtc gacagtctgt ataaaatcat tatgcggatc
    28441 gtgacgcttg ttttgcgttt gacaccttat ggagtatttg ctttgatgac aaacgttttg
    28501 gcaacaagtg attttgcagc tatcgtcaat ttaggtaaat ttgtcgttgc ttcttacgta
    28561 gcattgcttg ctgttttgat tgtccactca ctgattttga ttggtgcaaa agtcaatcca
    28621 atcaactatt tcaaaaaagc ctttccggta ttgagctttg cgtttacttc acgttcaagt
    28681 gctggagctt tgccattgaa tatcgagact cagacaaaag ctttaggagt agatgatgca
    28741 acagctaatt tctcagcaag ttttggtttg tctattggac aaaacggctg tgctggcgtt
    28801 tatccagcta tgttggcaac gatcgtcgca ccagcagctg gagtgaatgt ctttagtttg
    28861 gaatttattt tgatgcttgt ggcaattgta acaatcagtt cttttggtgt agctggagtc
    28921 ggtgggggcg caacatttgc ttcactgatc gttttagggg caatgaactt gccagtagca
    28981 atcgttgggt tagttatctc tgttgaacca ttaatcgata tggcacgtac agcagtaaac
    29041 gtcagcggta gcatggtagc tggtatcgtg acaagtgcac gcatcaaaga tttagatcgc
    29101 gatgtcatca atgatgattc taaagtgatc gatgtgaatg catagtcaaa aagaggttgt
    29161 gatagaagtg gtcagcttta agaaataaga agaagtttcc taaaaatagt ttctcaaatt
    29221 tttgtgaaat ttcggcttat ttccgagaag ctacttctga aacaccgtga ataagaggtt
    29281 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
    29341 tttacgtttg gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
    29401 ggtcgtgagc ctgccgtttc actctgagtc acaaagagca atttcaaaaa atagcttctc
    29461 atgtttgttg aatattgtgt cttgtgaccg aggagttgtc aggagaacac cgtttaccag
    29521 aggttgtgac ttgtttttag tcacgacctc tttttttatt tattccatat aaatacacat
    29581 tatttattcc gaaaagtgaa agcgcaaata acaaaaacaa ccaacttatt tattattaaa
    29641 gaaaagcgta tttaagtagg acgattctgt tgagagcttt cgtagatttt cccattttcc
    29701 tatggtaaac tgacaaagtt gagaatgtta gaacagatcg tttaggagga aacagatgtt
    29761 tacgaatgaa ataaaaatca tgctttatgt ggacgatgta gaaaaaaatg caacgttttg
    29821 gagagcagtt ggctttgcgg aaaaagatcg tcaagaaatg gatgggacgc ttataattga
    29881 agtaggagtt gaggacagtc aaacctcatt tctgttatat gatcgagcat ttgttgagtc
    29941 ccattcacct ggcacagcta ctagtgcccc atctttgatg ttttacagta aagatatctt
    30001 tggactatat aaaaaaatgc agacgcaatc agtcacatta ggtgatatgg tacagcttgg
    30061 agaagaatat gtctttaact ttgctgatcc cgatggcaac tactttgcgg ttacaggtaa
    30121 ataatagaaa taaagacttt gtttttctgt gcgaaacagg aaagcgaagt ctttttaatt
    30181 caaataataa tattatgtaa acaaaaaact ttccatgcgt aattatacac aagcaaataa
    30241 atcgtcaaat gcgaacgcct tctttatact gtttaggaac gaaaagaagg aaggaagaaa
    30301 tacatggatt atcaagtact gctttattac aattatacaa aaatcgaaga accagaggaa
    30361 tttgccaaaa atcatttagc cttttgcaaa tctttggatc ttaaaggtcg tattctagta
    30421 gctgaagaag gaataaatgg tactgtgtct ggaaccgttg aaaacacgac tgcctatatg
    30481 aaagcaatga aagctgatcc tcgatttgct gatacgatgt tcaagatcga tccaagcgaa
    30541 ggacatgcct ttaagaagat gtttgtaaga taccgtccag agcttgtaag tttaaagcta
    30601 gaagaggaca ttgatccaaa ccaagtgaca ggtgcctatc tttcaccgca agaatttaaa
    30661 gaagctattt tagatgaaca gacagtggtg attgatgcac ggaatgatta tgaatatgac
    30721 ttgggacatt ttcgaggggc tgtacgccca gatatacgca gttttcgaga gcttccagac
    30781 tggatccgcg agcacaaaga agaatttatg gaaaaacggg tagtgaccta ctgtactggt
    30841 ggcattcgtt gcgagaagtt ttctggttgg cttgtccgtg aaggattcaa agatgtcgga
    30901 cagctgcatg gcggtattgc tacttatggt aaggatcctg aagtccaagg agaattatgg
    30961 gacggcaaga tgtatgtgtt tgatgaacgg atcgctgtcg atatcaacca agtagataaa
    31021 caaatcgtcg gtcgcgactg gtttgacgga acaccatgtg aacgatatat caattgtagc
    31081 aatcctgagt gcaatcgtca gattcttgct tccattgaaa atgaagaaaa acatttagga
    31141 gcatgtagca tagagtgtgc gaaacatccg cgcaatcgct atgttcaaga ccatggaatc
    31201 tctccagaag aagcagaact gcggtttaca gctttaatta aatagaaaac aatagttttg
    31261 attgtcgacc atttccaata tgcgctatac tagcgttgag gatggtgatc cacatgaaat
    31321 cattgattta tgctattggg gatgtccatg gcaaacatga gctttttgaa aaacttattc
    31381 actattatga tccaaagatc catcagcttg ttttattagg ggatctcaat gatcgaggtc
    31441 ctcgtacaaa agaatgtttt ttattaggaa agcaattagt cgaagaaaca ggagccattt
    31501 acctccgggg aaaccatgaa gaatattttt tgcaatttct gcaatcaccg gaggactggt
    31561 atgcgcctta tatgagaaat ggtggaaaag aaacgtttga aagcctgatt cacccagggg
    31621 catcggagga atactcccca actgaaatct cgatgatgat ccaatcgaga tattcagaac
    31681 tgattaaatt tttagtccag cgccccttgt attatgaatg ggaaaattat ctttttgtcc
    31741 atgccggtgt ggatttgaca aagaaagact ggcgtaagac aagtctaaga gactttattt
    31801 ggataaggga accatttcat gaagggaaaa acaatacagg taagacaatt gtatttggac
    31861 atacgattac accaatgctc catggagata tgcagacaac agacctctgg attcaggatc
    31921 ataaaatcgg aatggacggt ggggctgtat tcggagggtc attgcatggg gttgtttttg
    31981 acaaggaagg tattcttcaa gatattgaat atcagaacaa cgaaggacct tggcatggaa
    32041 tctgactaat ggaaagaatg aaggagctgt gactttgttg cggctctttt tatttttata
    32101 gagaatattg tttctttcag tagtttaacc caggataaaa agtatgatag aataaagaag
    32161 caatttttag cagaaaagag gcattttaat gacggaaaat ttgaaccaga cgatcattca
    32221 gcttgtacaa aaagaacttt cagactatcg tcctaagcag ttgactactg tacttaattt
    32281 gttaaatgaa ggaaacactg ttccttttat cgcgcgttac cgaaaagaaa tgactggaag
    32341 tcttgatgaa gtccagattc gtgaaatcga agagcggtat gcctatcttg aaaacttaga
    32401 aaaacgtaaa actgaagtga ttcggctaat cgatgaacaa gggaaactga ctcccgagtt
    32461 agaagcagaa atcacccaag ccgtgaaaat gcaacaagtg gaagatttgt atcgtccata
    32521 caaacaaaaa cgacggacaa aagcaacgat tgctaaagaa aaaggacttg aaccactcgc
    32581 gaagtggctg atgcagctta cagatggaga ggtgcaatct gaagcagaaa aatatattga
    32641 caaagaaaaa gaagtctctt ctgccgaaga agcgttgcac ggtgctcatg agattattgc
    32701 tgaacaagtc agcgataatg caaaatttcg gacttggatt cggtcttata cgtataacaa
    32761 aggtatgtat gtcagcaatg tcaaggatga gcaagcagat gaaaaaggcg tatatgaaat
    32821 gtattatgac tttgcagaac ccgtccataa aatggtttct catcgaatat tagctactaa
    32881 tcgtggagaa aaagaagata ttctgaaagt attctttcaa gtagatgaag cagcaatttt
    32941 agcttacttg gatcgccaaa tagtcaaaaa tcctgcctct ccttcttctt cattcgtcag
    33001 agaagcctat caggatagtt ataaacgctt tattcagcca gcaatcgagc gagaactgcg
    33061 caatgaattg actgaaaagg cagatgagca agcaatcgct attttcggcg aaaacctccg
    33121 taatcttttg ttgcaaccgc cactaaaagg taaagttgtc ctgggatttg atccggctta
    33181 tcgtactgga tgtaaactag cagtcgtaga tgcgacaggg aaagttttag caatcgaagt
    33241 tatttatcct cataaacctg cagcgcaagc aaaaagagaa gctgctggac cagcttttat
    33301 ccaattaatt aataaatatc aagtagatat ggtagctatc ggaaatggta cagccagtcg
    33361 ggaatctgaa ctttttgtgg ctgagcagtt gaaatccgcc gaccataaaa cctattacgc
    33421 tatcgtcaac gaagcaggcg cttctgttta ctccgctagc gaaattgcac gaaaagaatt
    33481 tccgcatcta caagttgaag aaagaagtgc tgtttctatt gcccgtcgcc tgcaagatcc
    33541 tttagctgaa ttagtcaaaa tcgatcctaa agctgtcgga gtaggccagt accaacatga
    33601 cgtttctcaa aaacgtttgg ctgaacaatt ggattttgtt gtggaaacag cggttaacca
    33661 agtaggtgtg gatgtgaata cagcaagccc gcagctcttg cagcatatat caggattgaa
    33721 taaaacaact gcccaaaaca ttgtcattta tcgtgaagaa aacggagaat tcaccgcacg
    33781 aactcaattg aaaaaagtgc cacgcttagg accaaaagca tatgaacaag caatcggatt
    33841 cttgcgagtc cctggtggga aaaacatttt ggacaacaca ggtatccatc ccgaaagtta
    33901 ttctattgca aaagaaatat taacttcagt ccaactttca gaaaaagagc ttggtacaga
    33961 agaagcagta gaaaaattga cacgattatc cgtagagaaa ctggctgaaa gcctatcagt
    34021 cggagaagaa acgttagccg atattttagc tgggctgaca caacctggac gagatatgcg
    34081 tgatgagatg ccagcgccct tattacgtac agatgtattg agtatggaag acttgaaacc
    34141 agggatggaa ttgactggaa cggtccgtaa tgtcattgac ttcggcgcat ttgttgatat
    34201 cggtgtgaag caagacggac ttgtccatat atcgaaactc agcaaaaaat tcgtcaagca
    34261 tcctacagat gtcgtgtcag taggagatat cgtaacagtt tggatagaac aagtcgatac
    34321 gaaaaaaggc agaatcagtt tgacgatgct ttccccttat gaagagtaac aatgaggagt
    34381 aacaaaaatt gacacaagaa gaactgcagc agttagtaga agagatatca ttaaaaagct
    34441 tccagcttcc tttcaagcac caagcaatgt tcaatccacg cttacgtaca actggcgggc
    34501 gctatcattt aaacgatcat catttagatt ttaatgcgaa gctatttgaa gagtcagacg
    34561 aagagacgat tgcaggaatc atcaaacatg aactttgcca ttatcatctt catctagctg
    34621 gaagagggta tcagcataaa gatatggatt tcaagtatct tttgaaaaaa acaggagggt
    34681 tgcgctatgc accagccttg acgaagaaac agataaagat ggagtattat cgatgccaag
    34741 attgttctgc cacgatctgc cgcaagagaa gaatcgatgt acgcaaatat cgttgtggaa
    34801 gatgtcatgg agaaatcaaa tggattgaga caaaaagaga agactttaat gaagcacaaa
    34861 aaatcagatg aaaggataga aggcctttgg aaaaaagtga gcagaggaat ctaaaagact
    34921 ggaatcagct attaaatttt ttagccgcac ctgaagaggg aaattttgag gaactgacgg
    34981 cacctacact tattttagct ggaaactgtc ttcctatctt agctgataaa gcagcacaaa
    35041 tgtatttaaa tggccaagta gatcaattgt ttttggtcgg aggggtgggg catgcgacta
    35101 gaatccttta tgagaatttt gaaaagcaag gatttcattt tgaagatggc atgagtgaaa
    35161 gtgaaatctg tcgtcagtat ctaaaagaag tctatgattt gcccgataag gcttttctga
    35221 tagaatcgaa gtctacaaat tctggagaga acgccacttt ttcactggag attcttcatt
    35281 ccttaggagc tgtaccagaa aaaatcttat tgatgaatga tccgacactg caacgcagga
    35341 ctcgagcaac ttttgagaaa gtatggcaaa atgaacaaac tattttcgcg aatgctgtgc
    35401 catttgtgcc ggaaatcctt cacttttctg aagagatcat ctttacagca aaagaactga
    35461 atcatcaatg gcctaaggaa tatttccatg cgctagtatt aggagaaatg gaacgtctcc
    35521 atgacgatga gaacggctat ggtccgaaag gaaaagattt tattcctcac attgatatat
    35581 cagaggaagt ttggtcttct tacaccagaa ttaaagagtc aataaaaacg gatttttctc
    35641 gtacttgacc attttagcaa gcgaatcctc ctttccaaat aaatttggtt gaaattttca
    35701 atcgtttata agataatgag gagcgtgttg aatgtaatag ctaaaagaat ggacgctaga
    35761 taagagaaga ggattcaaat ggaaaatcaa aataaattgt atcattttgt tggtatcaaa
    35821 ggttcaggaa tgagctcctt ggctcttgtt ttacatgaac aaggtctaaa tgtccaagga
    35881 tcagacattg aaaaatattt ctttacacaa agagatttag aaaaagcaaa tatcacgatt
    35941 ctgccattta acgcagataa tgtaaaacca gggatgacga tcattgcagg aaatgcattt
    36001 cctgattcac atgaagaaat ccagcgagca aaagaattag gtttggaagt gattcgctat
    36061 cacgacttta ttggtcattt tatccaaaat tatacgagta tcgctgtaac aggctctcac
    36121 ggaaaaacaa gtacgactgg actgctctct catgtattga gtggtgtccg tccaacaagt
    36181 tatctaattg gagatggaac aggacatggt gatccgcaag cggaattctt ttcatttgag
    36241 gcctgtgaat atcgccgtca tttccttgcc tattcaccag actatgcaat catgaccaat
    36301 atcgattttg atcatccgga ttattataca agtatcgacg atgtatttac agctttccaa
    36361 acaatggctg gacaagtgaa aaaagctatt tttgcgtatg gtgacgatgc ttatttacgc
    36421 aaattaaaag caaatgtgcc gatttattat tatggtgtca cagaaaacga tgatatccaa
    36481 gcacgaaaca tcgaacgcac aacttctggt tctgcttttg atgtctatca tggggatgaa
    36541 tttgttgggc acttcactgt accagcattc ggtaaacaca atatcttaaa tgctttaggt
    36601 gttattgcag tagcttattt cgaaaaactg gatctgaaag aagttgcaga agaaatgctg
    36661 actttcccag gagtaaaacg tcgattcagt gaaaagatcg tagcagacat gaccgtagtc
    36721 gatgattatg cgcatcaccc tgcagagatc aaagcaacga ttgatggcgc acgccaaaaa
    36781 tatccagata aagaaataat tgcagttttc caaccccata cgtttacgcg tacgattgca
    36841 ttgatggatg aatttgcaga agcacttgat ttagctgata aagtttactt atgcgatatt
    36901 tttggttcgg ctcgggaaga acaaggaaac gttaagattg aagaccttgg agcgaaaatc
    36961 aaaaaaggtg gagaagtcat caaagaaaat aatgtatctc cattgttaga ttatcatgat
    37021 gcagtagtaa tcttcatggg tgctggagat gttcaaaaat ttgaacaagc gtatgaaaaa
    37081 cttttaagca gcacgacgaa aaatgtactg taatacaatt actgcagtgt ccttataaaa
    37141 atagaaagag ggggagtggc aaaaatcttg ccaagccccc ttttttgcaa cttatgataa
    37201 acggtgttca aaagcttgca tctgcggtaa taagcccaat caaatcaaaa acatgagaag
    37261 ctgttttttc ttggctgttc ttattatttg atgcaggaca gctttttcac gacctctgta
    37321 aactatagga atctgttaaa tcaagggagc agaacttgcc ttttacgcta cttttggtac
    37381 aataatgtat gattaaaaat aaactggagg gtttctatct tgaacatagg taagccgaga
    37441 aaactaggaa aaacaataaa tgaaatcgag gaaggcgact cgttatcctt aacagaatct
    37501 attgaagata accagctttt gctgtatttg ggattgacga atgatgcaaa tcctttgtat
    37561 atccaacatg actttgcaca aaaaacagaa tatcaacagc caatcgttcc atcagtgatg
    37621 ctgatgggga tcatcacaag tgcaatttca aagcatcttc ctggaccagg atcacatgtt
    37681 gtcaattttt ctgtcaattt tattgaaccg gttttccatt atgaaacact gacttttcag
    37741 tttgaggtca taaaagtaga taagatgaag gatgtggtga cgatttctgt agaagcggag
    37801 aatacgaacg aagatcgtgt gttggatgct gtcgttatgg tccaaccgcc acaagttcct
    37861 gaattggaaa tagcaatcga aagtgaaggt gaattgaatg aataatccaa gtacatcaat
    37921 gtcaggtatc aatcgttttt atgcaaaagt ttatggattt ttaggtatgg gacttgccct
    37981 tagtgcagtc acttcttact tggctctttg ggtatttcct ttgcaattag caacttttat
    38041 caataatttt ccactaggct ttgtgggtct gtggatcgtt gaactgattt tagtaatggt
    38101 tctaggcgta aaagcacaaa agaatccgag cttagcaatc ggtggattta ttgcctactc
    38161 tcttttgaat ggcataatat tagcggttac tcttgcatac tacgatattg gaacagtaac
    38221 gcaagctttc ttgacagcta ctttgatgtt catggggatg tctgttgtcg gagtaacaac
    38281 aaaaagagat cttagcgggg ttggaagagc aggacaggca gctttaatcg gagtgatcat
    38341 tgcactagtc ttgaacatct tcattttgag aagtagcgta gttgagctgt tcatctccct
    38401 actgttagtc gtcatctttg cagggattac agcttatgat aaccaaagca tccgtaatat
    38461 ctatgtccag acaaacggaa cagcacatag tggaattgct gtattcatgg cattgaagct
    38521 gtatttggac tttatcaact tgttcttggc gtttctgaga atctttggac ggaacaacta
    38581 aaaaagaaaa aaattcatca gttggggcta taccgaatta tttggtatgg tcccgctttt
    38641 tcttttcgca ttccgcagga ttctttgtta gaatgaataa gaactttaca gtaaggagtt
    38701 aagaagatga caaaaaataa attattactc gttgatggga acagtgtggc ttttcgcgca
    38761 ttctttgcgc ttcacaattc tcttgaacgt ttcaaaaata gaaacggtct tcatacaaat
    38821 gcaatctatg cattcaatac gatgtttgaa aatgttatgc aaaaagagca accgactcat
    38881 gttctggttg cgttcgatgc aggaaaaacg acttttcgga cagaaatgta tgcggagtac
    38941 aaaggtggaa gatccaaaac acctggcgaa ttcaaagaac aaatgccata tatacgtgat
    39001 ttgttgacag gactaggggt acagtattac gaattgccca attatgaagc agatgatatt
    39061 atcggtactt tggcagaaaa agtagacaaa gatcaatttg atgtcgtcgt gctttcaggt
    39121 gatcgggatt tgactcaatt agctagtaaa gaagtaaaag tggatatcac tgtaaaaggt
    39181 gtcagcgata tcgaaagcta tacaccagaa catgtagcgg aaaaatatga tgggttgacg
    39241 ccaaaacaga ttattgacat gaagggacta gccggagatg cctctgacaa tatccccggt
    39301 gtgactaaaa tcggtgaaaa aacagctatc aagctattga aacaatatgg ctcagtcgaa
    39361 ggaatttatg aacatatcga tgaaatgaag caaagcaaaa tgaaagaaaa tctgataaat
    39421 gacaaagagc aggctttctt atcgaaaaaa ttggcgacca tcaatacttc agcgcctgtg
    39481 gatgtctcta tcgactcatt gaagtatgaa gggaaaaatc tcgataaact cgttccgttt
    39541 tataaagaaa tggatttcaa tcagttcctt tctaaattga atatcgtaga agaagaagta
    39601 aaaatggatg atattttgtt tgaagtcgtt catgaattca aagaggaaat gtttactacg
    39661 gatatggcgt tgtacgtgga aatgatgggc gacaattatc atacagagga aatcgtcgga
    39721 gtagcgtggg ggacagaaaa gaaaatttat gtcacgaacg atctatctgt attcgaaaac
    39781 cgagcgttcc attcttggat cactgatccg actcggctga aaaaagtata tgatgcgaaa
    39841 cgaacttatg ttgctttaaa ccgctatact ggaaaaacga aaggaatcga ttttgatgtc
    39901 ttgctaggtg cataccttct cgatacgaat gaaaaaagca cagatatcga aggaatagct
    39961 gctcattacg gatacaacga tatccaatca gatgaagctg tatacggcaa aggggcgaaa
    40021 aaaggtttac cagaagaaga ggaactcttt tttgctcatt tagcgcgaaa agtagctgca
    40081 atcaatgcgt tgacaccaaa attgagccaa gaactggcag aaaaaaacca agaagatctt
    40141 ttccggaaaa tggaactgcc attgtctatc attttggcag agatggaaat aagcgggatc
    40201 aaagtagatg cgactcgctt acaagaaatg aagggcgagt tctctgctcg cttaagagag
    40261 atcgaacaaa aaatctacga agaagccggc gaagaattca atttgaattc accaaagcaa
    40321 ttaggagtga tcttatttga aaaaatggga ttacccgtta tcaaaaaaac aaaaactgga
    40381 tattccacag ccgtagatgt actggaacag cttcgtgaac aagcaccaat cgtagaggat
    40441 atcctcacct atcgtcaaat tgcaaaaatt caatctacct atgtcgaggg actgctgaaa
    40501 gtgatccaat cagatggaaa agtgcatacc cgttatgtcc agacattgac ccaaacagga
    40561 cgattaagct ctgttgatcc taatttgcag aacatcccga ttcgattaga tgaagggaga
    40621 aagatacgtc aggcatttgt tcctagagaa aaagactggc tgatttattc ttctgactat
    40681 tcacaaatcg agttacgcgt acttgcgcat atctcaaatg atgaacattt gaaagctgcc
    40741 ttcctagaag gtcaagatat ccatgccagc acagcgatgc gtgtatttgg aatcgaaaag
    40801 gcagaggatg tcacaccaaa tatgcgtagg caggcaaaag cagtgaattt tggtatcgtg
    40861 tatggcatct ctgattacgg actttcacaa aatctaggaa tcagccgaaa agcagcacag
    40921 caatacatcg atacgtactt tgaaaaatac ccaggtgtca aagaatatat ggaaagaatc
    40981 gtacgcgaag caaaagacca aggctacgtc gaaacgctgt atcaccgccg tcgatatctg
    41041 ccagacatca attcaagaaa ttacaatatc cgttcttttg cggaacgaac agctatcaat
    41101 acaccaatcc aaggaagtgc tgctgatatt ttgaaaatcg cgatgatcga attggataaa
    41161 cgattaaaag aaacaggtct tcaagctacg atgctgttac aagttcatga tgaacttgtc
    41221 ttcgaggtac cggaaaaaga acttgaatca ttagataaat tggtgaagga agttatggaa
    41281 caagctgttt ctcttcatgt accactgatc actgacagca gttggggaaa aacgtggtac
    41341 gaagcaaaat aaacattgtt taagacagag caatataatg aaggtgaaaa gaaaaaatgc
    41401 cagaattacc agaagtagaa accgttcgta aaggattgga acggttagta gtaggaaaaa
    41461 cgattcaaaa agttcaggtc ttgtggccca agatcatcga gcaaccagag acacccatct
    41521 ttgaggcttc tcttgttggc gaaaccatcc aatccattgg acgtagaggg aagtttttaa
    41581 tttttcatct cgatcattgt gaactgatct cacacttgcg aatggaagga aaatatcaat
    41641 ttacgaaaga aaatacacca atcgataaac atacgcatgt acttttcttt tttgaggatg
    41701 gaagccaact tcgctacaac gatgtacgaa aatttggtcg tatgacaatc gtcgaaaaag
    41761 gagcaagtgc aacttaccga ggaatcatga aattgggacc tgagcctttg ccagatagct
    41821 tcttattagc agatttcgca aatggactga aaaagtccca caaagcaatc aagccattgc
    41881 tgctagacca acgtctagtg actggtctag gtaatattta tgtagatgaa gcattatggg
    41941 aagcgaagat ccatccagaa caaccggcga acacactgcg ccccaaagag gtagaacaat
    42001 tacgtctatc gatcattgat gttttggatc gtgcaataga agccggaggt accacgattc
    42061 gcagttattt gaatgcatta ggagaatcag gcggatttca agtagcactg catgtctatc
    42121 agcaaactgg caaaccatgt atacgttgtg gaacgccgat cgtgaaaaca aaagtggcac
    42181 aaagggggac gcactattgt ccgaattgtc aaaggctaaa aatcgcaaag taggatttgt
    42241 tttaggtttg acgggtggaa tagctaccgg taaaagtacg gcagccaaag tctttcagtc
    42301 tcatggattt cctttgatcg atggcgatgt gattgccaga gaaactgttg agcctggaac
    42361 gcttgctctg aggaaaattg tatcagtgtt tggccaagag gtattgcagc ctgatgggca
    42421 actagatcga gggaaacttg ggatgattgt ttttccatca aaggaaaaac ggcaaaaact
    42481 tgatcagcta ttagatccgt ttatccgaaa agcgataaaa gatcaaatcg cctctctaag
    42541 ttccaatcat cctttagtaa ttgtagatat cccattgctc tacgaaggtc attatgacca
    42601 ctatatggat gcagtagctg tcgtttatac tacgccagaa actcagcttc agcgtttgat
    42661 gaaacgtaat caactgacgc tagaacaagc gcagcagaga attagtagtc aacttccaat
    42721 cgaagaaaaa aagcagcgtg cggatatttt gttcgacaac aatggaacaa aagaaatact
    42781 tgtgggacaa atcgaaaatt ggctttttgc taatcgcttt ctttgagtat ctgtgtcaat
    42841 tttgctatcc tgagtatata gaaagtgagg gatagcgatg ggatcattaa aaaatcaaaa
    42901 ggataaagtc gtaggtaacg caaaagaaaa aatcggcgag atgctcaata aagaaaatct
    42961 aatcgatgaa ggtcgtacgc aatcagaaac agcagaagaa aaagaaaagc agtaccaagc
    43021 aagaatcgat gaaatagaag accagcaggc agaagaagaa gcgaagcggc aagatttgac
    43081 tcaagaacga aaaaatccga cacctgatcg tagtaaaaca ccgctcccag aaagtgacca
    43141 cattcccgat gagcaaaaaa tcgaggaaca gcgaatgaaa cactatacag gaatcgaaga
    43201 gaagttataa aatagtaaaa aatcgagatg tgacttgaag cagatgagaa ggtgttcatc
    43261 tgcttcaagt tttttgtgct agatgactaa atcagctttt gtgtaagtta tttctcagta
    43321 atcaaggtga tcagtattgg aaaattgaaa tttagtaagg acaggggaca caattacgca
    43381 tgaaagtaga aaaaattgtt cttatatccg tgttttttgg gtaggacagt cgcgtttgtg
    43441 atttgtttcg tgttataata caactagttg taaagaaaga cacatccttt atataaaatg
    43501 cgatgcaaga ggtgagacaa aatgagatgt cccagatgtc aacataataa ttcacgagta
    43561 atcgacagcc gtcaagcaga tgatggacgt gcgatccgtc gccgtcgcga atgtgagaat
    43621 tgttcgttcc gattcactac atttgaacga attgaagctg ctccgttatt agtaatcaag
    43681 aaaaacgggg aacgcgaaga gttcaatcgc gataaaattt taagaggact catccgttca
    43741 gcagaaaaaa gacctgtagc tatggaacaa atggaacaaa tcgtcaacca tgtcgaaaac
    43801 cgcgtacgca gtctcggaga aaacgaagta tcgacgaatc taattggtga atatgtgatg
    43861 gaagatttag ttgatttaga cgaaatcgct tatattcgtt ttgcaagtgt ttatcgccag
    43921 ttcaaagata tgtctgtttt cttaaaagag ttgcaagata tcgtggataa agcaaaagaa
    43981 aataataaag agaataataa agaaaacaat taagaaacaa aaaggaggca tttccttgga
    44041 aaatgcttgg aaagagcttc aaccaaaaaa tatttaccaa ggaagaaaag cggctccgct
    44101 gagagaagac agcaagaacg ccttgctgta tctttatcag ccactaatcg gcggagaagc
    44161 cttgagtatc tatttcaccc tgttatcaga aatctctcaa gaatctggca aaggtcctga
    44221 agggatgcat gcagatctgc ttagtagtct aggctgcggt atcccgcaat tttatgatgc
    44281 aagaaaaaag ctagaaggaa tcggcttact ggatgtttat tttaaagaag atagtgatct
    44341 gggacctcgt tttatttatg agctgttaga accactcatt cctaaagcat ttttcaaaga
    44401 ctctgttctg gcttttcttc ttctggaaaa agttggtgaa cgacgattcc agaagcttac
    44461 ccaattcttt gaacctaaaa ggcatacagc agagggatat caaaaaatca caaaaaaatt
    44521 tttagatgtt tatcgcttca atgaagcagt gtacgctgct aatcaagaaa acatcgaatc
    44581 gctgaataaa tcttttgaag aatctgaatc agtagcatta cttgaagcag attcgacatt
    44641 cgattggcag tttttgaatg attggctggc aaaaaaacat attgcaagtc ttgatgatcc
    44701 aatcatcaaa caagtcgaca tgtatcatca gctttacggt ttcgatgaat tgacacttgg
    44761 cgaactgatc agtcaatctt atgactttag tgaacaaaaa gtatctgaaa aagaactgca
    44821 aaaagccatt ttggcaatgc agagacgtcc ggtgaagcaa acaaaagaaa cagttgcggt
    44881 ttctgaaaac agtaatgaac cctcgtcaaa cgaacatttg acaaacgcag cgcttcagct
    44941 gataaaagaa gcacagagca ttccaccgat gaaatatctg gaagcaatca agtcagaaaa
    45001 aggcggatat gtttctaaac aagaaaactg gttactgcaa gatcttgttt ctcaatcagg
    45061 tctttccagt agcgtgatca atgttttgct gaattatgta ctagtgatca agaataatgc
    45121 tagcttaaat gccagttatg taaatacagt ggcaaatgaa tgggcacaaa agaaaatcgc
    45181 cacagcagaa gaagcgatcc agcacattcg gcaaataagt aatcaagcaa aacaatcaaa
    45241 acaaaagaag caaacaaatt atcaaactgg caagcgaaat gttcgacggg aaaagcttcc
    45301 tgattgggtc aaccagccaa aagatgaaaa acagatcagc agcgagaaaa aagcagagat
    45361 tgaccgccgt tttaaagaat acctggcaaa aaaggagggt gatatctagt ggaagacgta
    45421 ggcaaagaat tgaagaggat catcagcaaa cgcaattatc aagaaaagta tgctgaaatg
    45481 atcgctgaag tcttaaatga tgcggatgtt caagcttttc taagcgcaca ccaagaagaa
    45541 ttgacacaag cggatattga aaaaagttac gctaaattgt atgaattcgt gcaagaaaag
    45601 cgtaaatatc aaaaaaacga tccaacgatg atcgctcccg gctatgagcc gaagctgacg
    45661 ttgaattttc attatgtgga tgtcacttat gtaccaacgg aagcattgtt agcacggcaa
    45721 agacaagaag aaatcagaag ccgagtaaaa gcaatggata tgccaaaaga tattcaatcg
    45781 gcttcttttg aaaactttga acgtacagat ggacgaggat atgcgagctt agaagcccta
    45841 gattttgtag agaaatatac tgctgatcca aaagggtatc ataaaggact gtatctagta
    45901 ggaagttttg gtattggaaa aacatattta ttaggagcaa cagcgagaga attagcgatt
    45961 gctggattca ccacgacgct agtccatttt ccgacatttg ccgttgagat gaaacaggca
    46021 atcggaaaag atcaagtggc agaaaagtta gatgcagtga aacgttcacc tattttgatg
    46081 atcgatgaca ttggtgcaga tgcgatgagt agttggatac gagacgaagt attcggtgtt
    46141 atccttcaat atcggatgca ggaacagtta ccaaccttct tctcatcgaa ttttacaatg
    46201 gatgaattgg aacagcattt gtccgtcact caacgtggag atgaagagcc gctaaaagcg
    46261 aaacggataa tggaacgcat acgttatttg acaaaagaaa tcgaaatgac tggtcgcaac
    46321 cgacgcaatg gttgaacagt tgacaagtcc aactgacatt tgacctagta gtaaaagcag
    46381 gtcagcgaat cattatatgg acagaaatga ccggagacaa tcaccgcaac aagtaatgca
    46441 gaaacttaaa cggaggattt tttatgaggg ctattttttc tagtatatct gtagttggtc
    46501 tattttttct tttagcaggc tgcggtagcg cagatagcaa cacaggttct gttgattcga
    46561 ttgatgcggg tcaaacagta aaaacaagca gtacgaaaac ggagagacaa agcatatttg
    46621 tcggaacgat tgaaagtatc actcaagtag aagcagaaga agaaacgctt cagttgaaac
    46681 tcgtcgatgt catggccaaa gaagatccag cgaatatggg aacgtcattc agcaatgacg
    46741 gagtgatctt aaatgctact gctgaaaaaa taagcgtgga cataaatgaa ttgaaaatcg
    46801 gtgacaaagt ccaatttaca ttagttgaga tgccgatcat gacgatgtct atcccaccgc
    46861 aagttcctgg agattcgatt attgatgttt cattgaacaa ataaccaata acattttttc
    46921 aaaaataaga aaatgttgtt ttatagaacg aaaaggtata ttttttaagg agatatggga
    46981 atttttttat tcctagtctc cttattttat ttggaaaatg ctataattat ctagaaggag
    47041 tgaagctatg aaccatttag atttatctca gcgcttagcc gcagtgggtg actttgtccc
    47101 tgaatccgct cgtttagcag acattggatc agatcacgct tacttacccg ttgcattgat
    47161 attaagagga aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc
    47221 agcacgtaag caagtcaaaa aagatggatt agaagaccgt atcaccgtac gccttgcaaa
    47281 tggtctagat gctatccagc cacaagatgg tattacagct gtcacgattg cgggtatggg
    47341 aggttcactg atccgagata tcctagaagc tggcaagttg aatcaacgct taagcggaca
    47401 agaacgtttg atccttcagc caaacatcgg agagcgtaca ttacgatctt ggctgcaaga
    47461 aaatcagtat aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat
    47521 cattgtagcc gaaaaagaac aaaaaccagt tacttacaat gaaatggagc tgatgttcgg
    47581 gccatttctg cttgaagaga aaagtgagat cttcaaatca aaatggacaa gagagcttca
    47641 acaaagaaaa gctatccgtg aacagctcaa acaagcagct aatcaagagg cacgtcaaaa
    47701 acaaatcgag caggaaatcc aaatgattga ggaggtttta aagccatgac aatcagcgga
    47761 cgcactttca tccaaaagtt tgaaagctat tgtcctcagt ggctagctga agaaggagac
    47821 ccagtcggac tgcatatcgg aacattggat aagccaatcc aacgagtaat gatgacatta
    47881 gacgttcgtc cagaagtggt cgaagaagca atcaagaaaa aaatcgattt gctgattgct
    47941 aaacatcctc caatctttcg tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa
    48001 atgtatgcag atttgttgaa acatgatatt gccgtctatg cagcacacac gaacatggat
    48061 attatttggg atggactgaa tgactggttt tgtgaacttc tcggtattga agtggagagt
    48121 tatttagtaa agactcatga gattcattat aaaaaactgg ccgtttatgt acctgtggat
    48181 catgcacaga aaatgcgtga agtattagct gcagctggtg ctggtacaca aggtgattat
    48241 accggtacaa gttttacctc gattggacac ggtcgattta ctccggaagc tggtgcacaa
    48301 ccagcgattg ggaaagtagg gaagacagaa caagttcaag aggcaaaagt tgaggttatt
    48361 ttgccggaaa ctatcgaaaa acaagtgatt caagccatgc ggtcagctca tccttatgag
    48421 gaacctgctt atgatctttt cgctatagat gaaccagtcg aaatgtttgg gttaggacga
    48481 gtaggtgaat tgccgcaaga aatttctata gaggcatttg tagaacaagt aaaagaagcg
    48541 ttccaactag atggactgag aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc
    48601 attgcgattt gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca
    48661 gatgtctata tcacgggaga tatctattat catacggctc atgatatgca aagcgctggg
    48721 ttgattgcaa ttgatcccgg tcattatatt gaatcgttat gcaagcaacg atttatcgag
    48781 aaatttgaat cttggaaaca agaagaaaat tgggatatta actttttcgt ttctgaaaca
    48841 aatacaaacc catttcaatt taattaaaag gagagaatac tatgtacgaa aatcttttgc
    48901 cacgtttttt acgatatgtc aaaacagaaa cacgctccga tgcaacaagc aaaacgactc
    48961 catcgactca gacacaagtt gcttttgcgc aagaattgaa aaaagaatta gaagagttag
    49021 gcttagctga tgttttctat aatgaagata acggattcgt tatgggaaca ttaccaagca
    49081 atgttgatca tgaagtgcgt tccattggat ttatttccca catggataca gcagatttta
    49141 atgcagtagg agtcaatcca caaatcattg aaaactatga tggcgagtct acgatcgtac
    49201 tagatgcgga agaacgctat acattgaata caaaagattt tccaaatttg aaaaattatg
    49261 aaggccacac attgatcaca acagatggca gtacattact gggagcagat gacaaatcag
    49321 ggattgcgga aatcatgact gcaatggaga tcttgatcaa taatccttct atccctcacg
    49381 gtaccatcaa agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg
    49441 atgttgagca attcgatgta gattttgctt atacagtcga tggtggtcct ttaggtgagt
    49501 tgcagtatga aacgtttagt gctgctcaag caaatattac gatccaaggg aaaaatgtac
    49561 atccagggac ggcaaaagat acaatgatca atgctctaca gttagcaatc gattttcata
    49621 atcagcttcc agccgatgaa gttccagaaa aaacggaagg atacgaaggt ttcttccatt
    49681 tgatggcatt ggatgggaat cctgaagaag cttctatgag ttatatcatt cgcgatcatg
    49741 atcgtgaaaa attcgaagca cgcaaagcac aaatcacaca tatccaagaa gagttgaatc
    49801 agcgatttga tcaagaacga atcaaagtag atatgtatga tcaatattac aacatgaaag
    49861 aaatcattga aaaagatatg agcatcgtgg aattagcgaa acaagcaatg atcgagcttg
    49921 gtatctctcc agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa atctcttaca
    49981 tgggtattcc gacgccaaat ctatttgccg gaggagaaaa tatgcacgga cgattcgaat
    50041 ttgtttcttt acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga
    50101 acacaaagag ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg
    50161 aatgtcatct gtctaaggct aaagggcagg acaatggaat tggttgtccc gccctctttt
    50221 ttgaagagta aataggaggt aaagaatgga aaagctgcta gttttttata acaaaagttc
    50281 agggaaagat gagggtgaga aattagcacg ctggtttcaa gattatgtaa aagaaaagca
    50341 gccagaatta tatgtagaac tagctgaaac tggcccatcg attgatgatg cggaattagt
    50401 caagaaagca gaagccatag aagctgatac tttagtaatt ataggtggag acggcacgat
    50461 tcatcatatc gtccaagcgt tccaaaaaac attatctcaa taccatgtcg gattacttcc
    50521 aggtggaact gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc
    50581 tgctgacgtg atcctggaag gcaatgtacg gaaaatagac tatggtcaag tcaatgatga
    50641 agtcattatc tctacactga caatcggaat cttagcagat acagcagttt ggatcagtca
    50701 agaagaaaaa cagcgttacg gctcgtggat ctttatcaag aagtttttta agttattgct
    50761 gaagaagaaa aaatatcatt tggatttaga aacagacgag gagcattgga aaggaaaatc
    50821 ccaactgttg acagtcacga tgtctaactc cgtgggcggt tttacaaatt ttgacgactc
    50881 agcgacccta gatgatggaa aatttcatgt aacgatcgta ccttccttag atattttccg
    50941 atttattttt tatttgccta gaatcatgag aggcaaattc tatacgatcc ccggcattac
    51001 ttatttcaca gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac
    51061 ggatggagac acgacagatg atcttccagt tgaattatct gttgtatcaa aaggattaac
    51121 agtttattca ccaaagaaag aagaaaaagc ttagaaatag tagttaacag aaagttggaa
    51181 gaagagtaat ggatgtattg ttacacaatc ctttgtggtt ttaccttttc attgtgaatt
    51241 tttacttatt ttgtctgatg gggtatgata aacatcaggc tagaactagc ggctggcgtg
    51301 ttcctgagaa taatttgata tttgtcagcc tgttaggcgg tggtttggga ggattgatct
    51361 ctcgaaaagt tttccatcat aaaactcgga aaaagaagtt tactatagga ttcgctgcag
    51421 gtgtacttgt tgatttgatt ctcatttatt ttttccatta aggagcagga gatgtcgtct
    51481 aaaggaacga aaactaaaat cattctgaat ctgcttttgc tgattgtgat ttttgccatt
    51541 atttattatt taatcaatca atcatttgca gatatctttg ccgaattgct gtctaccagt
    51601 gttgttgttt tggcagcggc tgttatatta gggacagttt accaaatcgc tgaagggcgc
    51661 tcaatcaaag aaattgctga acctttcgcg ccgaatttta caacgaaaga tgggttcttg
    51721 acctctagct atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg
    51781 gagatttatt tttataacaa aaaaggaatg aagctatccc agggagcagg ggtgacagca
    51841 ctccatatga ttatgtacaa actggcagtg atcacttatg cattagctgg cttggtcatc
    51901 caattctctc ttttttattc taatggtcca tcaatgatct ggtttatcat cgctggtatc
    51961 cttttgacga tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa
    52021 atattttttg tcagcttatc gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg
    52081 gatacatgca atcagcagat ctactcttta agggaaacgg tccaaaccat tatccaagac
    52141 aggactgcgc tattgcggat ttatctatgg aatatgttaa aacttgtatt ttggtatgta
    52201 cttccttatt tgattctgat tgaaaaccat cctaaaatag attttctact gaccatttcg
    52261 tttatcagct ttgcagtagt gctttcaggg gttattccta cgcctgctgg tattggttcc
    52321 tttgaatttg tctatttgtt attattcaga ccagttgttg gaacagttga cgcagtttct
    52381 tcgcttttac tgtatcgttt cagttccttt atcttgccat ttctctatgg atttgtgtat
    52441 gtgattgctg aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat
    52501 aaagaaaatc aagggtaggt cttttataag atctaccctt tttatcaaat caaagaaaaa
    52561 cgttatggat aagataattt tacgtttgtt tggctctttt tttatataca tacaatcaat
    52621 tttgtttttt taagatttat ataagtcgag aaaaaaagtc aaaaaactat gtgtatattt
    52681 tgaccaaaat tgaccaaagc gatataatag tttttgtaaa gatcaaagaa ataagtgatc
    52741 ttacctttaa ttaggagggt atacctgtat gaatattgag aaaatgacta cgacgcttca
    52801 agaagcgatt gctgaagctc aacagattgc ggtcactcga aaacatcaag atatcgacat
    52861 tgcccatgta tggaaaatct ttttacaatc taatcatttt ggtcgaaatt tctatacgga
    52921 tgccggattg gatgtcgaat cttttgaaca tgaaattgat cgtctactgg atgagtatcc
    52981 agttgtttct ggtggaaacg tccaatatgg acaaaacctc agccagaatt tctttcgatt
    53041 attgaatgaa gcagaccaaa tacgtgaaag cttcggcgac gagtttcttt caacggaagt
    53101 cgttatttta gcgttgatga aattaaaaaa ttatccgtta actgtttatt tgaataaaaa
    53161 cggattatct gaaaaagaat tgcgtaaaaa catcgaagag atgagaggag gagaaagagt
    53221 gacctcacaa aaccaagaag aacaatacaa agcattagaa aaatacggcg ttgatttagt
    53281 acagcaagtc aaaagcggaa aaatggatcc aatcatcggt cgtgatgaag aaatccgtga
    53341 tgtcatccgt attctttcac gaaaaacaaa aaataatcct gttctgatag gagaacccgg
    53401 tgtagggaaa acggcaatcg ttgaagggct tgctcaacgt atcgtacgca aagatgtgcc
    53461 tgaaaattta aaagataaaa cgattttttc attagatatg ggtgcattga tcgctggagc
    53521 aaaattccgt ggtgaatttg aagaaagact aaaagcagtc ttgaaagaag tcaaaaaaag
    53581 tgatggacgt atcctcttgt tcatcgatga aatccacaat atcgtagggg caggaaaaac
    53641 agaaggaagt atggatgccg gaaatctgtt gaaaccaatg cttgctcgcg gtgaattaca
    53701 tttgatcggt gccacaactt tagatgagta ccgtcaatat atggaaaaag acaaagcgtt
    53761 ggaacgtcgc tttcaaaagg tattagtcaa agaaccaaca gtggaagata cgatcagtat
    53821 tctgcgtgga ttaaaagaac gttttgagat ccaccatggt gttaacatcc atgacaacgc
    53881 attagtagca gcagctactt tatctgaccg ttatattact gatcggttct taccggacaa
    53941 agcaatcgat ttgatcgatg aagccagtgc aacgattcgg gtagaaatga actctatgcc
    54001 aacggaatta gatcaagtta ctagacgttt gatgcagttg gaaatcgaag aagctgcact
    54061 gaaaaaagaa tcagatgatg caagtaaaaa acgcttgaaa aatttgcaag aagaattagc
    54121 tgaacttcgt gaagaagcaa atgcgatgaa gatgcagtgg gaaacagaaa aagaagaagt
    54181 caattctgtt tcagcaaaac gtgctgaaat cgataaagca aaacacgaat tagaagatgc
    54241 agaaaataac tatgatttag aacgcgctgc tgtattgcgt catggtacga tacctcaatt
    54301 agaaaaagaa ttgaaggaat tagaagctaa agcaaaagac agcgagatta agatggttca
    54361 agagtccgtt accgaaaatg agattgctca agtcgttggc agattaacgg gtatccccgt
    54421 aacgaaactt gtagaaggtg aacgagaaaa actaattaaa ttgaatgaaa cacttcataa
    54481 acgagtgatt ggtcaagatg aagcagttga tgcggtcagt gacgcggtaa ttcgttcacg
    54541 ggctggattg caagacccaa atcgtcctct aggttctttc cttttcctag gaccaactgg
    54601 tgtcggaaaa accgaattag cgaaagcgtt agctgagaac ttgtttgact cagaagatca
    54661 tatggtacgg atcgatatga gtgaatatat ggaaaaacat gctgtgtctc gtctagtcgg
    54721 agctcctcca ggctatgtag gttacgaaga aggtggtcaa ttaacggaag ctgtcagacg
    54781 aaatccttat acaatcgtct tgttggatga aatcgaaaaa gcgcatcccg atgtcttcaa
    54841 tatcttgtta caagtcctag atgacggacg cttgactgat tcaaaagggc gagtagtaga
    54901 tttcaaaaat acggttctga tcatgaccag caatatcggt tcacagttac tgctggaagg
    54961 cgtgactgct gatggaacga ttccagaagc agtggctgaa caagtcaaca cgctattacg
    55021 tggaaacttc aaaccagaat tcttgaaccg aatcgacgac acgattttat ttacaccatt
    55081 gagcctagac aacgtgaaag gaatcgttga caaaatggtg gcgcaattgg cacaacgtct
    55141 ggaacatcaa gaaatcctgc tgacaatcag tgatgaagcc aaaacgtgga ttgctgaaaa
    55201 tgcttatgaa ccagcatatg gtgcaagacc attgaaacga tttatcacta aggaagtaga
    55261 aacaccacta gctaaagaaa tcgttgcagg acatgttatg cctaaatcaa aagtaacgat
    55321 tactttactt gatggacaac ttcatttcaa aacagaagag ctagaagaaa tcgtctaatc
    55381 ttccattgag actataaaag aaaaaagggg tctgacacaa ctagtgtcag acctcctttt
    55441 ttcaaataaa cagtgttcca gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag
    55501 ctgcccgctt ctgtcacaac ctcttttatt aggtattttt cactttttct aatttataac
    55561 tttcaaagta acgctcatct tttttacttt gagaccatgc aaacaatccc attaaggcag
    55621 cacctatcgt atttgtcagc aagtcgccca tcgtatccat caatgctgct cgtccgacaa
    55681 acagtgtgcc atcagaagca gcaaaacgtt gaagattcat gcctaagaac tggtcgcata
    55741 agaattccca gaactcccaa aacacgccgc aaagtccagc aaacgcaaag ccgaataaga
    55801 ggaacaacca aggactggtt tttgatatct cagcatcttt catcaagtaa ccaatcaatc
    55861 catagccaag agctgtcagg accattggac tcaccgcatg taagattttg tcccaaaaag
    55921 agatgatact gatcaaatgc atccctgtgc ctaaaaagac agagatgaac aagaaaaacc
    55981 agtaaaacaa aacgattgct gctggtattt taagtttgaa aactttcaaa atgacttgtg
    56041 gcatgaaaat caatgcaatc cctaataaag attccacgat aaatgtgatt ccttgagaag
    56101 tcgaatattt ttgtgtgata aattcataaa aattatagca gacaacgaat acaccaaaag
    56161 caagtaaagc tatttgcacc tttttgattt ctttaatcac tgaaagtccc tccatctaaa
    56221 aatcttttta cttctgaaag caactcaatt tctgaagaaa ccacttttcc gttcaatttg
    56281 atcaaaccta ttgtatataa gttcaggtag tgaaattgat tttcagctgt ttcttgtagt
    56341 gcagcgattt ttttcgggtg ggttccgcct tgctgtcgag tatcagtata taagccgatc
    56401 actgggatgc ctttcgcata agccacaccg atttccgatg ctaccccggc atcgatcgtc
    56461 agcccatcta gtaaagcaat cattaaatca ctagccaaca ctttttctgt gtcagcaaga
    56521 gcaatcattc gactgtctgc ataagcagtt ttgtcattga ttgcttcatt ttcttgagga
    56581 agatagatcg aaagctcaga agagagctct ctgatttctt taactaactg ttggttatat
    56641 aacaaatcac ttttagcaaa cattggtgcg gcaaaataaa ttttcatcta aacatcatcc
    56701 tttttatcaa aaataatcgc taaagtaagc ttccatgttt ggaatgatac gctctagtga
    56761 cttcagtgtt tcttcatctg tttctaaccg ttcgattctt gccaaataag aagcacggcg
    56821 ataattcatc atcaagcaag tcaatgtttg gatatccagt cgaacggatt ttcctatagg
    56881 ttcatttaag acacggactt gtccctgttc atcccatgtt aaagcgaaga caccattatt
    56941 ccaaggagcg acaggatctt caatgatgaa atgaaaagca tcagctgttc caacaaacgg
    57001 aaaccgttga aggaattctt ttacatcaac gatccgagcc ataaaatatg gttcgatctg
    57061 ctctttgatc tgactatctt caatcaaaaa tgctaaaggc tcatttttat aaatatctcc
    57121 atgtacccag tagatcatgg aaaaatgtgc ggaaataaag ttccataagc catttcgagc
    57181 ttcttgatca agatagaaca tttcttttac gtgaaatatt tcttcagcta cccagtagaa
    57241 cagaacacct cttggttcat ggttgctatc gtagtagaca gcggctgtgc gctcatcttc
    57301 attttcgaaa cgccaatact cttcccaatg aaaatcactg cggaataaag caccatggtt
    57361 ttgttgggca aatttgtcat aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc
    57421 aaccatacca cttactggga tttgtttagg tagctgtgta tctctgattt taaaggaaag
    57481 tttatcagac atgatttccc atcctttacg ccgatagtag gggatactgt aaggatacaa
    57541 ataagagatc cattgcttat tcttgcgcat attatccaat gcaacgataa ttagatcttg
    57601 catcaatcca tggttagcat attctggata agttccaact ccagtcacac cacccatttt
    57661 ataacgtgca ccgtgaatat tgacttcaca aggatagata gcgatttgag agatcagttt
    57721 attttcgtgg aaccatccga acactttcga tacttccaaa atcggttttt tggaacggat
    57781 aaaagctcgt ttattctcaa acccgctttc ctcgatgtct gcttcagtca cttggaacac
    57841 ataggaaagc aattcattga attgatcgat atgttcttga gtgaccggtt ttaattcaag
    57901 attttctcta aaatcttttt gatccatttt ttcatctcca tttattagct tgataacagt
    57961 atagcaaaat tctacaaaaa aaacttctat agctgactaa ttaaaaactc tttaaattac
    58021 tgcttttcgt tggtataatg gaaagtgctg attatatgaa aagaggagac gctatggata
    58081 taaagaaaat gaaagaacgc caagagcaga ttcgtaattt ttcgatcatc gctcatatcg
    58141 accatgggaa atctacattg gccgatcgta ttttagaaat gacgcacact gtgacgtctc
    58201 gcgaaatgca agatcagctg ttagactcga tggatttgga acgtgaacgt ggaatcacga
    58261 ttaaattaaa tgctgtagaa ctcaattaca cagcgaaaaa tggagaaaca tatatctttc
    58321 acttgattga caccccaggg cacgtcgact tcacctatga agtctctcga agccttgctg
    58381 cctgtgaggg tgccgtttta gtggtcgatg cagcacaagg aatcgaagca caaacattgg
    58441 ccaatgttta tttagcgtta gacaatgatt tggagatcct gccagtcatc aataaaatcg
    58501 acctacccgc agcagatccc gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg
    58561 atgctagtga agctgtatta gccagtgcca aatcagggat tgggattgaa gatattctcg
    58621 agcagatcgt ggaatatgtt cctgcaccaa gtggagactt agatgcaccg ctaaaagctt
    58681 tgatctttga ctctatttat gatagttacc gcggtgttgt tctaaatgtc cggatcacgg
    58741 acggtatggt caaacccgga gacaaaatca aactgatgag taatggcaaa acatttgatg
    58801 ttacagaggt cggggtcttc tcgccaaaag cagttgctag agactttttg atggtcggtg
    58861 atgtcggtta tatcacagca agtatcaaaa ctgtccaaga tacacgagta ggggatacag
    58921 taacgttagc agacaatcct gccgctgaag ctttaccagg ttaccgaaaa atgaatccga
    58981 tggtttattg tggtctttat ccaattgata cttctcgtta caacgatttg cgggaagctt
    59041 tagaaaaact gcaattgaat gatgctgctt tacaattcga accagaaacc tctcaagcat
    59101 taggttttgg gttccgctgt ggattcctcg gacttcttca tatggatgtc gtccaagaac
    59161 gcttggaacg ggaattcaat ctagaattga tcacgactgc accatccgtt atttaccatg
    59221 ttaataaaac ggatggtacg actgctacag tagataaccc ggcagatttt cctgaacctg
    59281 taacgattca ggatgtagaa gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt
    59341 ttgtcggtgc agtaatggaa ctttctcaac gaaaacgggg agaattcatt acgatggatt
    59401 atttagatga ttaccgagta aatgtcgtct ataatattcc gctttctgaa attgtctttg
    59461 actttttcga caaattgaaa tcaagcacga aaggctatgc ctcattggat tatgaaatgt
    59521 ctggttatca aaaaagcaag ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg
    59581 atgcattaag ctttatcgtt caccgagact ttgcctatga acgtggaaaa gcaatcgttg
    59641 aaaaactgaa aaaactaatc ccacgccaac aatttgaggt gccaatccaa gcagctattg
    59701 gacaaaaaat cgttgcccgt tcagacatca aagccttgcg taaaaacgtc ttggctaaat
    59761 gttatggtgg agatgtttct cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga
    59821 agcggatgaa acaaatcggc tccgtcgaag ttccgcaaga agccttcatg gccgttctga
    59881 aaatggacga ggacgaaccg aagaaatagc aagggtttgt ttgaaaacag tgtaaaaaat
    59941 aaaccatgtt tacatggatt tgccaccggt ttgccaccgg tg
//
LOCUS       DANKAZ010000005        54197 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000005 DANKAZ010000000
VERSION     DANKAZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            23..796
                     /locus_tag="PWY67_000285"
     CDS             23..796
                     /locus_tag="PWY67_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056598.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(877..1446)
                     /gene="thiT"
                     /locus_tag="PWY67_000286"
     CDS             complement(877..1446)
                     /gene="thiT"
                     /locus_tag="PWY67_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL2056599.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1512..1614)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1620..2591)
                     /locus_tag="PWY67_000287"
     CDS             complement(1620..2591)
                     /locus_tag="PWY67_000287"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2056600.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2685..4058)
                     /gene="glmU"
                     /locus_tag="PWY67_000288"
     CDS             complement(2685..4058)
                     /gene="glmU"
                     /locus_tag="PWY67_000288"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL2056601.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4561..5379)
                     /gene="purR"
                     /locus_tag="PWY67_000289"
     CDS             complement(4561..5379)
                     /gene="purR"
                     /locus_tag="PWY67_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL2056602.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5546..6370)
                     /locus_tag="PWY67_000290"
     CDS             complement(5546..6370)
                     /locus_tag="PWY67_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2056603.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(6343..7023)
                     /locus_tag="PWY67_000291"
     CDS             complement(6343..7023)
                     /locus_tag="PWY67_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2056604.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(7046..7984)
                     /locus_tag="PWY67_000292"
     CDS             complement(7046..7984)
                     /locus_tag="PWY67_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL2056605.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(8229..9092)
                     /locus_tag="PWY67_000293"
     CDS             complement(8229..9092)
                     /locus_tag="PWY67_000293"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL2056606.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(9234..11807)
                     /gene="mprF"
                     /locus_tag="PWY67_000294"
     CDS             complement(9234..11807)
                     /gene="mprF"
                     /locus_tag="PWY67_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2056607.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11948..12754
                     /gene="proC"
                     /locus_tag="PWY67_000295"
     CDS             11948..12754
                     /gene="proC"
                     /locus_tag="PWY67_000295"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL2056608.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12833..14389)
                     /gene="rny"
                     /locus_tag="PWY67_000296"
     CDS             complement(12833..14389)
                     /gene="rny"
                     /locus_tag="PWY67_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL2056609.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14800..15849)
                     /gene="recA"
                     /locus_tag="PWY67_000297"
     CDS             complement(14800..15849)
                     /gene="recA"
                     /locus_tag="PWY67_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL2056610.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(15958..17199)
                     /locus_tag="PWY67_000298"
     CDS             complement(15958..17199)
                     /locus_tag="PWY67_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL2056611.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(17439..18017)
                     /gene="pgsA"
                     /locus_tag="PWY67_000299"
     CDS             complement(17439..18017)
                     /gene="pgsA"
                     /locus_tag="PWY67_000299"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL2056612.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(18226..19149)
                     /locus_tag="PWY67_000300"
     CDS             complement(18226..19149)
                     /locus_tag="PWY67_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2056613.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(19888..21174)
                     /locus_tag="PWY67_000301"
     CDS             complement(19888..21174)
                     /locus_tag="PWY67_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2056614.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(21167..22435)
                     /locus_tag="PWY67_000302"
     CDS             complement(21167..22435)
                     /locus_tag="PWY67_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2056615.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            22687..23148
                     /gene="mscL"
                     /locus_tag="PWY67_000303"
     CDS             22687..23148
                     /gene="mscL"
                     /locus_tag="PWY67_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL2056616.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(23208..23654)
                     /locus_tag="PWY67_000304"
     CDS             complement(23208..23654)
                     /locus_tag="PWY67_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2056617.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(23747..23992)
                     /locus_tag="PWY67_000305"
     CDS             complement(23747..23992)
                     /locus_tag="PWY67_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL2056618.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(24107..24685)
                     /locus_tag="PWY67_000306"
     CDS             complement(24107..24685)
                     /locus_tag="PWY67_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2056619.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(24762..25292)
                     /locus_tag="PWY67_000307"
     CDS             complement(24762..25292)
                     /locus_tag="PWY67_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL2056620.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(25296..26156)
                     /gene="rlmB"
                     /locus_tag="PWY67_000308"
     CDS             complement(25296..26156)
                     /gene="rlmB"
                     /locus_tag="PWY67_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL2056621.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(26149..26544)
                     /locus_tag="PWY67_000309"
     CDS             complement(26149..26544)
                     /locus_tag="PWY67_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL2056622.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(26541..27953)
                     /locus_tag="PWY67_000310"
     CDS             complement(26541..27953)
                     /locus_tag="PWY67_000310"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL2056623.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(27950..28486)
                     /gene="cysE"
                     /locus_tag="PWY67_000311"
     CDS             complement(27950..28486)
                     /gene="cysE"
                     /locus_tag="PWY67_000311"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL2056624.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(28572..28811)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28808..30268)
                     /locus_tag="PWY67_000312"
     CDS             complement(28808..30268)
                     /locus_tag="PWY67_000312"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL2056625.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(30438..31595)
                     /locus_tag="PWY67_000313"
     CDS             complement(30438..31595)
                     /locus_tag="PWY67_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL2056626.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(31629..32999)
                     /gene="radA"
                     /locus_tag="PWY67_000314"
     CDS             complement(31629..32999)
                     /gene="radA"
                     /locus_tag="PWY67_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL2056627.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(33062..33589)
                     /locus_tag="PWY67_000315"
     CDS             complement(33062..33589)
                     /locus_tag="PWY67_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL2056628.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            33819..34625
                     /gene="proB"
                     /locus_tag="PWY67_000316"
     CDS             33819..34625
                     /gene="proB"
                     /locus_tag="PWY67_000316"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL2056629.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            34622..35869
                     /locus_tag="PWY67_000317"
     CDS             34622..35869
                     /locus_tag="PWY67_000317"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL2056630.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            35945..36289
                     /locus_tag="PWY67_000318"
     CDS             35945..36289
                     /locus_tag="PWY67_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056631.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(36406..36732)
                     /locus_tag="PWY67_000319"
     CDS             complement(36406..36732)
                     /locus_tag="PWY67_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056632.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(37347..38549)
                     /locus_tag="PWY67_000320"
     CDS             complement(37347..38549)
                     /locus_tag="PWY67_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2056633.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            38666..39181
                     /locus_tag="PWY67_000321"
     CDS             38666..39181
                     /locus_tag="PWY67_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2056634.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(39380..39826)
                     /locus_tag="PWY67_000322"
     CDS             complement(39380..39826)
                     /locus_tag="PWY67_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2056635.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(39873..41351)
                     /gene="msr(C)"
                     /locus_tag="PWY67_000323"
     CDS             complement(39873..41351)
                     /gene="msr(C)"
                     /locus_tag="PWY67_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2056636.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(41705..42334)
                     /locus_tag="PWY67_000324"
     CDS             complement(41705..42334)
                     /locus_tag="PWY67_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2056637.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(42318..42782)
                     /locus_tag="PWY67_000325"
     CDS             complement(42318..42782)
                     /locus_tag="PWY67_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2056638.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(43269..44537)
                     /locus_tag="PWY67_000326"
     CDS             complement(43269..44537)
                     /locus_tag="PWY67_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2056639.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(44768..45505)
                     /locus_tag="PWY67_000327"
     CDS             complement(44768..45505)
                     /locus_tag="PWY67_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2056640.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(45572..46141)
                     /locus_tag="PWY67_000328"
     CDS             complement(45572..46141)
                     /locus_tag="PWY67_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL2056641.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            46269..47141
                     /locus_tag="PWY67_000329"
     CDS             46269..47141
                     /locus_tag="PWY67_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2056642.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            47483..48355
                     /locus_tag="PWY67_000330"
     CDS             47483..48355
                     /locus_tag="PWY67_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2056643.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            48352..50310
                     /locus_tag="PWY67_000331"
     CDS             48352..50310
                     /locus_tag="PWY67_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2056644.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(50642..51493)
                     /locus_tag="PWY67_000332"
     CDS             complement(50642..51493)
                     /locus_tag="PWY67_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2056645.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            51616..52083
                     /locus_tag="PWY67_000333"
     CDS             51616..52083
                     /locus_tag="PWY67_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL2056646.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(52346..52828)
                     /locus_tag="PWY67_000334"
     CDS             complement(52346..52828)
                     /locus_tag="PWY67_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL2056647.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(52928..53791)
                     /locus_tag="PWY67_000335"
     CDS             complement(52928..53791)
                     /locus_tag="PWY67_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2056648.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        15477 a        11590 c         9662 g        17468 t
ORIGIN      
        1 aggaaataag aggaggaaca tgatgactga gtcagtttat gtacatattg ataccacaag
       61 taatgctgtt ttaacaaaag ggctgactgc tactgacttt gcaaacagta tcgtgcattt
      121 tcctcaaaac ttgctcttgc ttgatccatc tgcttctgct ggagaatatg aatcccatac
      181 agggctgaaa gtaatccgtg gtacagaaaa tattcagcgt ttcttttcct cttctcgtaa
      241 caggcgtgga ttcgatgaat tgaagtggat cgactttact gatttaacga tgctgaaaga
      301 attgacacct ttagaaattt cagaattatt atattttggt catatgaaaa cacatcttca
      361 ttcgccgttt ttttataaac tacagaataa ttttgtatac ttcgatctta atgatcagct
      421 gaatcggatt tattatcgct atttagatga attttatagg atttttgcta gtaaattaag
      481 ttcgatcttg tcagataaga tcaatgtaaa aaaatctttt ttcaaaaaag aaacacctgt
      541 tgaaaaaatc agtgtggaat tattgaaaga aatgcgtaat ttgatgcagg aagggatcat
      601 tttttctttt gatcaagttg gtttgatcga tggtaaatat cgtatcccaa tctatgtgat
      661 tgaagattat atttggaaat taaaaggcac ccggtatcga gaagaggaaa tcatcgctac
      721 tttaatctac tctgtcaccg atcgacactg gcaattggta gaagatgccg atctttctta
      781 tctgccattg aagtagatct atttcttatc aaaaaataaa aaggaaacaa gaaactagga
      841 catgattctg tctcaagttt cttgcttctt ttttatttac agctcctgtc gtttatctgg
      901 cacaaaaatc gaaggagtcg tttgttggat ccctaataaa acgatgatcg ttacaatagc
      961 tgtactcaac ccgctggcac cattcattac gaaagaaaag agccaaggat tcatccccca
     1021 tagtgcatac gctccccaga aaaccactcc tgcaataaaa tgccaaaaat atctggaaag
     1081 tgcgccaatc aatgcaccaa acacgatgct ttttctaatt tgtttcgtat tatcgtctac
     1141 atagcctttt tggacatttg aagccatgag ccctgctgcg cctgcaaaag tataggcaat
     1201 cgggtactca atcaacactt gaacgacact taaataataa acttgtccca acggaaaatg
     1261 taggatgccc caaatgaaac ctgccagcat ccctgccttt gtccctcttc ttaacgcaaa
     1321 aagagtcaat ggaatctgcc ctaatgaaat agaaaaactg ctgccgatct ggatcggaat
     1381 caacgacaac accatcgcta acgctgcaac aatcgttcct tctacccaaa ctctattttt
     1441 tcccataaaa aaacttcctc ctttgaataa aaaagtcaca aaggaggaag cagctttcga
     1501 aaatagtcaa acagctgttt attcacaatt cctacgctcg cataaccgat acaggtacga
     1561 agggtttaga aatagattca atctcagccc aattggactc ccctttgtga gtttatttat
     1621 tatttatttt ttgtttcaaa caatggacta actggtttgt tttcatggat acgtttgatt
     1681 gcatctccca ttagtccgcc aacacttact tcatcgattt tgtcgatttt acgatcttct
     1741 ggaagataga tcgagtctgt aacaactaga cgctcgatag ctgaatcttc tattctttgt
     1801 aaagcagggc cggataatac tggatgtgta catgaagcat acacactagt tgcaccagct
     1861 tctttcaaag cattggcagc caatgtgatc gtacctgctg tatcgatcat atcatcgatc
     1921 agtacacaag ttttgccttt gacttgtcca atgatattca ttacttctgc aacatttgct
     1981 tttggacggc gtttgtctat gatcgcaatc ggagatttca aaaattccgc taatttacgt
     2041 gcccgtgtga caccaccatg gtctggtgaa acaacgacaa catcatcgcc tttgattcct
     2101 ttttctaaga aataattggc aataagtggc gcacccatca aatgatcgac tggaatatca
     2161 aagaaacctt ggatttggac tgcatgaagg tccaaagtca gcattcttgt tgcaccagct
     2221 ttttgaatca tgtttgctac taatttcgct gtgattggtt cccgtgcacg tgctttacgg
     2281 tcttgacgcg catagccata ataaggcatg actacgttga ttgtttttgc acttgctcgt
     2341 ttcagtgcgt caatcatgat cagcaactcc atcaagttat cattgactgg gctgctcgtt
     2401 gattgaataa catagacatg cgatccacga atactttctt caatgtttac ttgaatttct
     2461 ccatcactaa attgagtaac agatgatttt cctaactcga cgccgactgc tgctgcaatt
     2521 ttctctgcta atgggcgatt ggaatttaac gcaaaaattt ttaatcttgg gtcaaagtaa
     2581 tgttttgaca tggggacctc cactttcttt ttcacaactc tagaattcta cctaataaat
     2641 agtagtcata ttttttaaat aaatcaagtg attttgcaaa agtcttagtt tagataaggg
     2701 agtttttttg catatccttc tttattgact tgtcttgctc tcgcaattgc cagcgcatat
     2761 tctggaatat tgtctgtaat tgttgacccg gcagcaatcg aactgttttt agctacctcg
     2821 acaggtccga tgatattcgt gctagaacca ataaagctgt ggtcgccgac agtcgtatga
     2881 tgtttgttct ttccatcata gttgacgaag acaacaccac atccgacatt gatttcttct
     2941 cccagcgtag catcaccaac ataagtcaag tgaccgactt tagttccttt gccgatttga
     3001 gcatttttca cttccacaaa gtttccgata tggacgtttt cgccaatttc agcttttggt
     3061 cgtaaatgtg cataaggtcc cacatctgca tgtttcttga catggctgct ttcaatgact
     3121 gagttttcaa tgacgacgtg atcctcaatc ctactgtcta cgatttttga atgtgacccg
     3181 atgacacaat cagaaccaat gactgtattt ccttggattt gtacacctgc ttcgatcaat
     3241 gtgtctggac cgatttctac gcctgcatcg atgtatgtcg ttgctgaatc gacaaagctt
     3301 acgccattga ccatatgcat ctggttaatt cgctttctca tgatctcatt tgctttagct
     3361 aacgcgatcc gatcattgac tcccattgat tcttcaaaat catccgtttg ataagcagca
     3421 actgtatggc cttcttcttt taagatttcg atgatatctg ttagatagta ttcaccttga
     3481 gcattgtttg ttccaacttt gcttaacgca tcgaacaaag cttcgttgtc aaaacagtaa
     3541 gttcctgtat tgatttcttg tacaagagct tcttctggtg ttgcatcttt ttgttccacg
     3601 attttttcta cgatcccaat atggtcacgg atgattcgtc catatcctgt tgggttttct
     3661 gcttgtgccg tcaagatcgt tgcacttgca ttttttcctt ggtgatattc aaaaagattg
     3721 ttcagcgttt cagtagtcaa taatggtgta tcgccactga taactaacgt tgttcctttt
     3781 tttccttcca agaaagaagc agcttgcaaa acagcatgtc ctgttcccaa ttgttctgct
     3841 tgtaacgcat atttactgcg ttctcctagt tgtgctttaa cttgttctgc tccgtgtcca
     3901 acgatcgtaa tcacttcatc tggtttcgtt tcactgaccc gattaatgat atgttctacc
     3961 ataggtttac cagaaacggg atgtaaaact ttatataatt ttgacttcat gcgggtacct
     4021 ttccctgccg ccaaaataat tgcataacgt gcgtccaact gcgtcactcc taaatatagt
     4081 ttgctgattt cagtttatcc cagtataccc attttttcaa caaaacaagt gattttcttt
     4141 tatcccagcg ttttctggat aaaaaaatag atagaccata acgggcctat ctatttggta
     4201 accggtgctc tcctgccaac tcctcggtca caagtcacac tactcataaa acatgaaaag
     4261 ctgttttttg aaatcgtttc ttgtgactta gagttaaacg gtaggcttac aacctcttca
     4321 ttaaacgttt ttcaatcagc tgaccttcgg tattagctca ataaacggca aaatatgagg
     4381 agcaattttt ccgtttattg agctaatact caggacagga cgctgatttc acaacctcta
     4441 gataaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatga aaaaatgtgg
     4501 aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcggctttt tcacaacctc
     4561 ttattctgaa aaataatttc ctggcacaac cgtaatattt ttcgtttgag tatctacatc
     4621 tttgacataa agcagtgacg tataatcatc gatggcgcga tgaccgttga attttgattc
     4681 ggcaaataca gtgatgccaa caagttctga ttcgaattct tcaatcaagc tcttcattcc
     4741 gttgactgtt ccgccgcctt tcatgaaatc atccactaca aggacacgtg aaccacgttt
     4801 caaacttctc ttagacagtt ccatcttttc aattctttca gaagatccag acacatagtt
     4861 gacacttact gttgatcctt ctgttatttt tgagtcccgg cgtacaataa cgaatggcgc
     4921 attcagataa taggacacag cttgggcaat aggtacgcct tttgtggcta cagtcattac
     4981 agcatcgatt ttttcaccaa ggtatttaga agcaatgatt cttccaactt gacgcagcag
     5041 ttcaggttct cctaacaaat cagagagata aacatatcct cctggaagca gacggtcttg
     5101 ttctgataga cgttctgcta gagattcaat atattttttc gcctcttcat atgaaatttc
     5161 agggatgaac aatacaccac ctgcagcacc cgggattgtt tccaaggttc cataccctcg
     5221 ttctttaaat gttttcttga tgattcccaa atcttcactg atagaagatt tagcagattc
     5281 ataacgttcg gcaaagcttg ttaaggaaat caattcatgc gggtgattca ataaatactg
     5341 tgtcatgtca actagacgtt cactgcgacg tactttcacc tattccaact ccttttaaga
     5401 aattttatcg ttttacatta tataagtttt tcttcaaaaa ttcgagcatt ttatgaaaaa
     5461 ataagaaaat agttcggtat ttattaaaat aaaaagaagc ctataaaaaa tttttcgttt
     5521 ttataaggct tcttttcttt ataatttagt tattttttct tctgaaggca accaatattc
     5581 gtttgatgat atttacaatc aggaacaaag caataaagac taatgtgatt gttgctcctg
     5641 gaggtgtatc tagataaaac gagctagtta gtcctgacag catccctaga aatcctacac
     5701 aaacactgat agcaatcacg acattgaagc ttttgcctaa tcgcataccg attgctgcgg
     5761 gaagaaccat gattgcggat atcaaaagtg caccggcaat cgggatcatc acagcaatag
     5821 ccacaccagt aatcacattg aacatcatcg acatcaaatg aacgggtagc ccatccacgt
     5881 gggcggtatc ttcatcaaaa gtcagaacat acatcggccg tttgaataaa aagaacaata
     5941 taccgatggc aacgaataag attcctaaaa agatgacttg ctcccatgta atggtcacga
     6001 tcgaaccaaa cagataggat tggatacttg ttgctgaatt ccctccgctc aaattcatca
     6061 atactaacgc tagcgccaaa ccaccagcca ttaaaatggc aatggagatt tctgaatagg
     6121 ttcggtagat cgttcgcaga tactccaata tcacagctgc taatacaacg actagcatgg
     6181 tcgtaagtgt aggattaagg ttcaaaaaga atcctaacgc tacccctgct aaagagacat
     6241 gagaaagcgt atcagccatc agcgattgtc ttctgatcac taaaaacaca cctagcatcg
     6301 gggcgattcc ggcaatgaag atagcggcaa gaaacgctct tctcatgaat tcatatgaaa
     6361 gcaacgccat tgagaatcct cctttctcac cagtcggatc tgtctgtccg catactgttt
     6421 gatatcttca tgatcatggg tgatcattag aactgatttc ccatggacat gcgcactatg
     6481 tcggagaaga cgataaaatt catttctgga ttcttcatcc atacctgttg ttggttcatc
     6541 aagaacaaat aagtcagggt cagtagcaaa aatacgcgct aaactgattc gctgcttttg
     6601 tccgccagac aattcaccta ttcgccgatt tcgcatatcc cacatcccta cagcttctaa
     6661 agcacgctgt acatgttgat gatctttttc agagagacgc ttgaaccagc ggcctcttgg
     6721 atatcttcct gaacggacta attcgatcac cgtactagga aaacctgcat tgaaagatgc
     6781 aacttgttga ggaatataac cgatacttaa tttttttcct tcttgatttt tctcagcaat
     6841 ctttatcatt ccttttgaag gtttcagtaa tcctaacgat gctttcacca aggttgactt
     6901 ggctgctcca ttttctccag ttaaaatgac aaattcaccc gcatctacat gataagagac
     6961 atcttctagt acaggttcat catcatagta aaaggtcaaa tcagatattt cgatataacg
     7021 caccttacaa cctccattct aaaacttaat tgatactttc ttttaaagct ttcagatttt
     7081 tttccatgac agaaatataa ttttcgcctg ctttgatttg ttcattagtc aagctttcta
     7141 aaggattcaa cacttctagc ttcactcctg tttcattcga caatgtctca gctaccttcg
     7201 aagaggcatt ttcttcaaaa taaatcactt tgatgttgtt tttcttcaca aattctttta
     7261 gctcagctaa gcgacttggt gtgggttcct gatctggtga aacgcccgaa atcgctactt
     7321 gttttaagtc gtattgattc gctaaataag caaaagcagc atgctgagtg acaaatgtgc
     7381 ggtttgttgc atcttttagc tcatcggagt atttttgatc cagtgcttca agctttttga
     7441 tataagcatc ggcattcttc gtaaaagttt cctttttatc tggatatttt tcgcctaatt
     7501 cttcagcaat cgttctgact tcttttatag ctaataccgg atcgagccaa acatgtggat
     7561 cgtattcatg tgaatgacca tgcccttctt ctgattcttc atgttcgtct gcttcttgcg
     7621 attctaaacg gtcgattcct ttagctgctt cgatcatcaa ggtctgtttg ctatccactg
     7681 aatcctttaa cgaatccaca aaaaattcca tgtctgaact attatacacg aaaacatctg
     7741 catcggaaat ttctgctctt tcttttgcac ttggttcgaa atcatgcggc tccgttccag
     7801 cagggatcaa aagtttcacg tttccttcat cccctacgat ctcttttgta aaatcataca
     7861 tcggataaaa agtcgtaacg atcgtcaaat cctctttttt gtctgcttcc ttgttcgtat
     7921 ttccacatgc tgcaaacagt aaagcaccta tcattaccgt agctgtcaaa agatattttt
     7981 tcatttatat gctcctttca atgaacaaat cggaatttta cgatttatta acgtgaacta
     8041 atgtatcaaa ataaaaaaac acagtcaata gaaatccatc aatttctgat ttaagcatgt
     8101 atattcttga ttaaatcgta aaatggatga gttgctgctc atatctagcc atttcactac
     8161 aataaaaaac tgaagaatac cattttagga ttcctcagtt tttcatcacc tattttactt
     8221 cttatatttt attgcagggt tcgtactaaa taaacttcct cacaaaaacc ttttaaccca
     8281 ttatatactc tctgcgctct tgaataatgt tgacacaagg caaaaacggt tggtccgctt
     8341 ccactcatca atgcagcatc tgctccgtac ttgatcatac gctctttgat ttgatggatg
     8401 actggatgcc gctttcccgt tatatcttct aaactgtttc ccattttttt gatcatctgc
     8461 tgataatctt gctctcggat ggcttcagca agtccgtgga tgtcttgatg ttggatggct
     8521 tgcgtatcta cttctctaaa cactgtacgt gtagagacac tcattcttgg tttgaccaac
     8581 acaacccagc actgtggcat agaaggtaat acttggattt cttccccttt gccaccgaca
     8641 aatgccgtac ttccgtagac acagtaagga acatctgtac cgacttccaa accgatttct
     8701 gccagttctt ctaacgaaca gcccagattc cataattggt tgatccctct tagagcagct
     8761 gcacagtctg tacttcctcc gcctaatcca gctgctactg gaatattctt cttaacaaag
     8821 atcttgacac cggttttgat attgtattta ttttttagca ataaagcagc ttgataaaca
     8881 ttgttccgtt catctaccgg taaaaatgct ttatttgttt ctacaatgat tttgtcttct
     8941 gttatttctt caaaaaatag atgatctgcc aagtctacgc tcgacatcac catttccaac
     9001 tcatgatacc catcttgccg tctatataat acatccagtc ctaaatttat cttggctggc
     9061 gctttttcaa tcaattccat cctctcaccc attcgaaaag tctaagtcgc aagtattcta
     9121 tcatagggaa aaataaaagt atagcttatg tctgcctagt ggttgatttt gtcatcgttt
     9181 caacagcttt cacagaattt tctcttcata atccaaacaa gttctagtga ttcttataat
     9241 tctgtgagca cttcttctgc caaactcgga acatttcctt tttcttttcc tatcagactg
     9301 agtagctgca tggtagcaaa tccgaaatta cttcctttcc tataagcaaa ataacgtcct
     9361 tcccaatgat cgacatattt taatttcttt tcataagcag actggaagga atagaaaaaa
     9421 ttaccataat tatatacgat atgcatgact ttttcttgaa agaaagaaaa atctgtttct
     9481 ccaacattag ctaatggagc taagcctaga ttggcttccg agtagcccat tttctcgtat
     9541 acaataaaca gattggctaa cagatagtca gctaattcct cagattcttt aggtaagacc
     9601 cgcaacaaat catatcccgc ctgcttcttc gtcatcggct gagcggtgat gaagccaacg
     9661 actttttgat ctttacggac aataccgaca ccgctagata atacatatga tttatcaaat
     9721 cgtcccacag agaagtattt ttctcgtgca ccttgaagcc atttttgtga aacttcattt
     9781 acagcttgat gtaattcttc cggcagttga tcgtaccaag taaaatggta tccttctttc
     9841 atcaaacgtt gatattcaag ttgctgactc agccgagaca agtcttctgt tgctagatca
     9901 acaatccctg cttcacctac tttcgtgaag tgaaaaccca attcatgaag catcactacg
     9961 tagctttctg atatcttata aaacgcaagc tgatagccta aacaatctgc ttgtttcata
    10021 aactgattcg ttgctgcccg catcatttta ggattgccaa ctggatctcc taaaacaaag
    10081 catttgtttc ctttgatctg ataaccaaaa acgacttgat cttggtcgtc ttcttggtaa
    10141 taaaaataac tgtaacctga caagcgaagc tgatggcttg tagctgtgcc tccgtatgtt
    10201 tccaataaac ggtcaaaacg gtctttttcg tactcactgc caagcttcgt agtatcttca
    10261 gctaaatatt gataaagcgc cactaaagct agcaatgaga tgcccaaacc aataagccct
    10321 gagaaccaca catcatctga aggaaacaaa ataaaatgat ttcctgttat tcgaccgact
    10381 aaaaagcgtc cgctgtgata gcctgcaatt gcgtaaacga taaacaaaaa gccgaacaag
    10441 ctagcatcaa agattatagc tccccaagaa taaacgaatt ttgttcgata aaattctttt
    10501 ctggataacc acacagctaa cagaatcaag acatatacca atatcaactg ccaagataac
    10561 gtacgtgtga ttgtatttaa aataccaaaa gatagtaaag caatcgtagg cagaaatgcc
    10621 tttttgactt ttactgaaag tgctctagca aggccgagta ataaaaagcc aaccaataga
    10681 ttcactgtct gatctaaaaa attgaatgag aacggcaata atacttgaaa tagccgactg
    10741 attgcagaca gattagtgat tgttgataga agtaccatca tgatccccgc aaaatagaca
    10801 gcacttacga gcacgatatg cgcaaccttt tggaaaaaca gtcgcggaat gttatctaaa
    10861 aatcgattga ttttgactcc tgtatgtgtg atgaacaata gaagtcccgt cataaaaggt
    10921 agaacataat aaaacaaccg ataatataat agccaaacta ctgctacatc ttgccgaaca
    10981 gacaattgac tcattcctaa gatcatcaaa acgtcaaaag tccccattcc acctgggacc
    11041 atcgtcagca ttcctatcaa tgtagcaatc atgaacatag gatagatcga aagcatcgaa
    11101 atatctattc tcatcaactt gccgatgatc aaaaataccg caagggctcc cgtccattgt
    11161 ccaaaggatg ccagtagcag accaagcaag ccttttggat aaaactcgaa gaacaatgtc
    11221 tttctcttta gataagcaaa aaagaaaatg actggtgcta aaaaactacc gccgaaaagc
    11281 cagatccaat agcttcgaaa aatactttct gactgaataa aaaagacatc aataaatgta
    11341 agaaacgacc atacagacaa accagtcagc ataaatagtg cgacttttga tacagtggct
    11401 aaaaccattt ttctatttgt ctgctttcca taaaaactag cccgcagact tgctcctacc
    11461 acaccaccaa aaccggcgag attgttgatc gtgtttgtta cccacgcagc aagaagaaac
    11521 tcttttcttt ccatttttgg ttttccttgt ttttctaaga ctctgatcgt cacccaatca
    11581 tacaacaaca tcggagtgac acccagcagt ccagccagaa tcatgagcag gatcgtcatg
    11641 cggctttgtt gtcccatcct ttgccaaata tcttgccaac tcatgccatt agcaatattt
    11701 gcgacttgat ttgccacaaa gactaacacg gagccgaaaa aaatcagttt taaaatcaga
    11761 ctatgttcct ttatccattg cagcatcaag tatcgtttat ttttcacatt ttcccattcc
    11821 taacttgtat acttttgtgt cataccccaa tgttatctta tttattttaa tttttccact
    11881 ttagacctgt tctttgcttg cgatgataga aaaagaaaga tatcctaatg ctaaaggaga
    11941 ggataacatg aaagtaggat ttatcggcac agggaatatg gcacaagcta ttattgatgg
    12001 tttattaaaa aaaggaacag tctcgaaaga agaggtgttc gtttccagtg gacattttga
    12061 aaatgcacaa aaatttgctg ctgaaactgg cgtccatgca tgcaaaagta atcaggaagt
    12121 tgcatcacaa tcagatatcg ttcttttagc cgtgaaacca aaaatcattg aaaaagtatt
    12181 ggaagaacta agagaatata gccagaaagt cttattcatt tctattgctt caggaaaatc
    12241 attaatagat ttagaaacag cgcttgaaga tcctcaggcc tccatcgtac gtgtgatgcc
    12301 gaatgtcaat gtcagcgtag gagcaggcgt ttctgcgatt tgcaaaaatg aagctgccac
    12361 ggaggaacag cttcagcaag ctacgatgat ttttgaagca atcggcagcg tctatccttt
    12421 agcagaatca gatttcagta cattcattgg ccttgctggc agctcacccg cattcgttta
    12481 catgctgatc gatgcgatgg gacggactgg tgtactgaat ggtttgtcca aacagacagc
    12541 cactgagatt gctgcaaaag caattatggg ttcttgtcaa aaattccttt caagtgataa
    12601 aaatccatgg gaacttgtag accaagtttc ttctcctggc ggtacaactg ttgcaggtgt
    12661 agttgcctta gaagaagcag gattcatccc agcaatcatc aaaggaatca atacgacgat
    12721 cgagaaagat cttgagatgt tgaataaaaa ataatgatag caatgttttt tcgtgcctta
    12781 caaaaaaaga agagctgtgg cttttgccgc ggctcttctt ttgtattttt tattatttag
    12841 cataatcagt tgcacgagtt tcccgaataa cgattacttt gatgtgtcca ggataatcca
    12901 gttcttcttc gattcgttta cgaatatcac gtactaaacg aacagcgtct aaatccgaga
    12961 tttcttctgg cttgaccatt acccgaactt cgcgtccagc ttgaactgcg taacttgatt
    13021 ctacaccggc gaagctgttc gaaatatttt ccagacttct taaacgacgg atgtagtttt
    13081 ctaatgattc acttcgcgca ccaggtcttg ctgctgagag cgcatctgct gcagctacaa
    13141 ggacagagat aacagaagta gcttccacat ctccatgatg agaagcgatc gcgttgatta
    13201 ctactgggtt ttctttgtac ttggcagcta attccgcacc gatctcaacg tgagatcctt
    13261 caatctcatg gtcaagtgct ttcccaatat catggagcaa cccggcacgt tttgctaatt
    13321 ggatatcttc gccaagctct gctgctaaga taccagctaa ttttgcaacc tcgaccgaat
    13381 gcttcaagac gttttgtcca taactagttc tgaatcgcag acgtccgatg atcttgatta
    13441 gatctggatg aagtgtatga gcaccgactt caaacgctgc ctcttccccg tattcgcgaa
    13501 tacgttcatc catttcttta cgagatttct cgaccatttc ttcgatacgc gctggatgga
    13561 ttcgtccatc ttgtatcagt ttttccaagg tcatccgtgc gatttctctt cggattggat
    13621 cgaatccgga aagaacaact gcttccggtg tatcatcgat gattaaatcg ataccagtca
    13681 gcgtctcaag cgttcgaata ttacgacctt ctcgtccaat gatacgacct ttcatttcat
    13741 cattcggaag ggtaacgact gaaacagtcg tttctgaaac ttgatctgct gcgcagcgtt
    13801 gaatcgctaa tgataataag tttttcgctt tgcgatctgc ttcttctttg gcacgctgtt
    13861 cagattcttt taccattaga gttaactcat gattcagctc ttcttcagtc gatttcatga
    13921 tgacatctcg tgcgtcctct tttgataaac cagcaatttt ttctagttct ttttgttgat
    13981 cttcaatcat tttttcaacg tctttttctc gctcttcaat taattgctgt tttgaactaa
    14041 gtgcttcttc tttactttca agtgagttct cgcgtttttc taacgagtca tctttacgat
    14101 tcagcgtttg ttcgcgttga atcaaacgat tctcttgaga ttttaattct aacttacttt
    14161 ctctcagctc actttcaact tcagaacggt atttctgatt ttcttcctta gcttctaaca
    14221 atgcttcttt ttttagagtt tctgcttctt tttttgcact atcgatgatt ccagcagctg
    14281 atgattgtgc accagctatt tctttctcgt gacgtgactt tgcgaccata aaccctaaac
    14341 caagaccgac aattaaaccg atgatagcga ggagaatgtt gaataccata aaatccacct
    14401 ccatactatc tttttcattt ttatcatcta tacagcttct atgataaatt attctaaact
    14461 cgtcaatatg catcttcgca tatatgtgta caaaaaacat gcacaacttt attctaatgt
    14521 ttgtaccccg tagtgtcaac tttaaataaa caaagaaacg gctttctact tattcattaa
    14581 acgatggttt ttaaaataaa tgacactagt ttcatatagg taaaccttta aattgagaaa
    14641 cttttctatt aaattattct ttgtagatga acagatattt ttattttatg tacccactcg
    14701 tataattaaa taactcacga tttctttatt caataaaaaa acggcaggtc aaggagagta
    14761 cctactcttc agaccagccg tttttgttta tcataatttt tattcttcta ctaaaggcaa
    14821 ttcttcttgt cctttttcat cttctgggac agcgacttct tcaccaatac cataagctgc
    14881 tctaactaga gcagaaactt ctgccatcat ttcaggatga ttagccatat agattttagc
    14941 gttttcccgt ccttgaccaa tccgatcttc tttataagaa taccaagcac cgcttttatc
    15001 aacaatgtct ttttctactg ccatgtctag aagttctcct tcttgtgaga tccctaaacc
    15061 atacatgaca tctacttcag cgattttgaa aggaggtgct actttgtttt ttacgacttt
    15121 gatctttgta cggttaccaa caatatctgt accttgtttc aattgttctg cacgacgtac
    15181 ttccaaacgg atcgttgcgt agaatttcaa tgcgcgtcct ccaggagtga tttctggatt
    15241 cccaaacatc acgcctactt tttcacggat ttggttgatg aaaatagcga ttgtttttgt
    15301 tttgttgatc gaaccagaga gtttacgcaa tgcttgagac atcaaacgtg cttgtaaccc
    15361 gacatgtgag tcacccattt cgccgtcgat ttctgctcgt ggaactaaag cagcaactga
    15421 atcgactact acgatatcta cagccccact tgatactaaa gcatcagcga tctctagacc
    15481 ttgttctcct gtgtcaggct gtgaaagaag tagttcatcg atattcacac ctaatttttg
    15541 tgcatattgc ggatctaacg catgctcagc atcaatgaaa gcggccgttc cgccattttt
    15601 ttgtacttct gcaatagcgt gtagtgcaac tgttgtttta cctgaacttt caggtccata
    15661 tacttcgatg atacgtccac gggggtaacc gccgacacct aaagcgacat ctaacgcaag
    15721 agagccactt ggaattgtag agatttgttg gtcgattttt tcgcccaatt tcataataga
    15781 gcctttacca tagctctttt caattttttt cagtgcagca tctaaggctg ctttacgatc
    15841 atctgccaat acgataatcc tccttatatt tacaaatcag agcaacattt cattatcctt
    15901 atcatatctt ttttagtgaa aaaaagcaag aaaaaaccga acaaatattc gcatttatca
    15961 ttttcttaaa agctgttttc tcaaaagatc aaatccttgc ataaccgcat tatatctgat
    16021 agcagcacga tctctggcaa agtgatattt ttccgaaacc acgccgtctt tagatgaaat
    16081 agcgatccat actgttcctg cagattgacc ttctaacgtg tccggtccag ccactccggt
    16141 gaaagctaca ctgtaatctg ttcttgctat ctcacgtgct tgaatggcca tctgctccac
    16201 acattctttg ctgactgttc catattctgc taatagctcc ggatcaatct ccagaaaacc
    16261 agcttttgtt tggagcgagt aggtaacgaa tccgcctgga aatacttcag atacaccagc
    16321 aatgtcgcct aatgcggctt ggaaggcgcc ggctgtcaaa ctttccgctg ctgttacagt
    16381 ctgcttgttt tctttcagta gttccactac gacttttgct agcgaattat catcgccgta
    16441 tccataaaaa tattccccta ctcgttcttg gattttttct tctaatgcta aaagagcttg
    16501 attccctgcc tgaacatcat ttgttttcac tgtcagtcgt aatgtcactt catttggttt
    16561 ggcatatggt gcgatcgttg gattgatctg tgtctcaatc agatctttta gctcagtgac
    16621 cagtcgcgat tcaccaatcc cataaaagcg caaaacttta gaaattagtt tttcttcaga
    16681 aggaaaacgt tcctctagta aaggacgtac ttgttccaca aacattggtt tcaactcatt
    16741 aggcggaccg ggaagaagga tatacgcatg tgtatctgtt tgataaaaag tccctaaagc
    16801 aagccctgta cgatttggta gaggaacccc gccttcgata atctgggact gttgcagatt
    16861 gtttttggtc atttttcgat gggttgtttc aaaatatgcc aataattttt tgtatccttc
    16921 cgtattttgt atgagagact taccgaggtg agccgcagtt gcttcttttg tcaggtcatc
    16981 ttctgtcggt cctaatccgc cacataagag tatgagttca cttcttgttt cagctagagc
    17041 aatcaactct tctaaacgct gctgattatc tcctacaact gtttgataat aaacttcgat
    17101 acctagatca gctagctgtt ctgacaagaa agtggcattc gtattcacga cttgtcctag
    17161 taaaagttct gttccgacag ctattatttc tgctttcatt acttcccgct cccttatata
    17221 gtatacgctg tatttttata tttttttatt taaaaagagg gcaaggagct gtgacaaaaa
    17281 tcttgtcaca gctccttgtt ccaaataaac agtgttctcc tgccaaaacc tcggtcataa
    17341 gtcacaatat acataaaaca tgaaaagctg ttttttgaaa ttatcccttg tgactcagag
    17401 ttagacggca ggctcacaac ctctccacta aacgatactt acattgatcc tttgaacaca
    17461 ccgctgtttt tgatgaagta atccacacct gaatagatag taaagatcag acaagcataa
    17521 agcatgattt gatctactgg aataccgatc aggctgaatg ggatattatt gataaacagc
    17581 aagataatag caaccatttg tgtagccgtc ttgacttttc ccggccaagc tgcagccatg
    17641 acttctccat gttcgacaag tagtaaacgc aagcctgtga cggctaattc acgacagaca
    17701 atgatcgcta ctacccaacc aggggccttt ccttggtcaa ccaacatgat aaaagcagtc
    17761 ataactagca ttttatctgc caacgggtcc gcaaacttgc caaaattagt gacaaggcct
    17821 cgcgcacggg caatttttcc atctaaccag tctgtgatac tagctaaagc aaaaataact
    17881 gcacctacta gctgtgtgac agccagtgtg gtgtctccta cgttaaattg tccccaatct
    17941 aaaggtaaac ctacaaccaa aatgaaaatc gggatcatac aaattctaag tacagttaat
    18001 ttatttggta aattcacact atcgactcct ttcttcccta ttctacgtga ctatcagcga
    18061 agtttccacc gatattattt acttttccaa atgaatttat tcaaaggagt atttttattg
    18121 aaattgatct ttttgaatta acgaagaagg accgcgacac agatttgtca cgatccttct
    18181 tttttatacc tacaagtaaa cagatgcgta aactaattta tacgatcatt ctgcgtattg
    18241 aagagtaagg ttcacatttt ttggactctg attgttttcc cctggattaa ctggtacgga
    18301 ttctccattc gctttgatcg ttgcattgct tgctgcacca atcgtgatgg tcgcatttgt
    18361 tgctgtttcc ggtaacacgg tactctgtgt ttcatttgct gccagtgtcc cttgataaac
    18421 ataggcattg tctactagca cacctaacca tacacgattg acagcagtaa attcaagggt
    18481 tacaggcttg gtagcgtctg tgatatttac tgcgatggca ctgccagaat cttctcctgt
    18541 agctattgcc attttctttt cttcacttga ttttgttgtt gattctactg tagaagactc
    18601 tttggtctgc gtgctttgct ctgaggaaga ggactcctcg actgatcctt ctactttaag
    18661 cgaagaagct gtgtctccga ttattggatc agcttgacga tcttgccaag tcatatagcc
    18721 cacgacaacg acaatcgcca aagcaaccaa tcctaataaa atgacaggaa gacttgtcca
    18781 aaagcgactt ggatgcgatt cttcctgatg cattgctttt ctagaagtac ttactgtttc
    18841 aggctgcgga cgttcaggga ccgatcctgt caatttgctt ttcccatcaa aaacatcgac
    18901 taaatggtct ccatcttctc ctacagcttc agcgtattgc cgaataaatg cccgcacgta
    18961 aaatttacca ggcaattggt caaaatcatc attgtcgatt gcttccaaat aacgtttttg
    19021 aatttttgta atctgctgta attcatctaa agatattttt ttattcaggc gtgcttgccg
    19081 taatttttct ccgatggttt caaaagccac tcgttcatct tctccagtct gtttgattct
    19141 ccgaagcatc tgctgttgca acgaaagacg ttatgtttgg gtgctgtgac aaaagtcatg
    19201 tcacagctcc ttttttccga ataaacattg ttctcctgcc aactcctcgg tcacaagtca
    19261 caatatccaa taaatataag aagctgtttt ttgaacttgt tccttgtgac tcggagttaa
    19321 acggcaggct cacgacctct ttcttaaacg gtgttcaatc agctgttcct cggtattagc
    19381 tcaaaaaccg gcaaaatata aggagatatt tttccgtatg tttctctaat actcaggtca
    19441 aaacgctgat ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat
    19501 aagtccaacc aagttaaaaa cattaagaag ctgtttttac ttggctgttc ttattacttg
    19561 atgcagggca gctttttcac aacctcttgg tacacggtgt tccagaagta gcttcttcgg
    19621 caataagccg aaatatcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
    19681 ttgaagctga ccacttctgt cacaacctct ttgattactt cgtttgattt tattttaata
    19741 taagttgatt aaaagagagc atgatttgtt ttattttctc actaattttt ccaacacaac
    19801 taagaatacc ataaaagctg attttggagg aggttcctac tacaagtcta aaacgaaatt
    19861 taagactttt cctcataaaa tactggttta ttttggtctc atgtaaaact cgctgaaggc
    19921 ctcttcactt ataaatgctt ccccagctgc cagtacgtct ttcatttgga tcgaatcgat
    19981 gatttcagga agatcaaata aagttcgatc gccaaataaa ctttgcgtaa attgattggc
    20041 aatatattcg atcgaattta atgattggaa atattgtcca agcattttct tcttcagcaa
    20101 atcgagattt gtttctgaca cttccggatc atcagcgaat ccaagaatga tttctttgac
    20161 tttttctgct gctttctctg gttcgtccgt atctccgctg aagtccgcaa agtgaaattc
    20221 acgatccaag ctgaattcaa aaccaaagct gtcatcgata attccttgat tgtacatcgc
    20281 tagatagttt cgagaagtat tgcctaaaat catttggaac aacagattga ttgctgtttt
    20341 atagcgtaac agttctgttc cttcttgcgg caatgtgtct aatcctttaa taccaagtac
    20401 aaatttatct cgtgtaatgg ctgcttccaa tgcactttgt ttgatgattt cttttgtgtt
    20461 ttcagggaaa taacgtacaa tttcttgctt tggagggaaa ttttttgctt cttggttttc
    20521 ccgaataagt ttcatcaatt tctcaggctc catcttacca actacaaata aaaccatatt
    20581 gcttggctga taaaacgtcc gatagcacgt atacaagtct tgagctgtga ttttgtcgat
    20641 actttcaacc gtgcccgcaa tatcgatatg caatgggtgt gtaggataca agttatttag
    20701 tatgccaaaa aacatccgcc agttagggtc gtcttcatac atttggattt cctgaccgat
    20761 gattcctttt tccttgttta cagtttcttc tgtgaagtaa ggtgcttgta caaaatcgat
    20821 cagagtcgtc agattctttt ctacctgatc cgtcgtagaa aacagatagc tggttttcgt
    20881 aaaacttgtg aaagcattgg cggaagctcc ttgtttccca aataattgaa agacatcacc
    20941 atcttctttt tcaaacaact tatgttctaa aaaatgggca attccatctg gaactttcac
    21001 cttttctttt tcaccatagg gaataaattc gttatcgatc gaaccataat tcgtactgaa
    21061 caaaccatat gtcttgtgat agtcattttt aggcaacaaa tacacggtca aaccgtttgg
    21121 cagtacttca tgataaagtg tttcattgat ttgctcatat tctgttttat tcatttgcat
    21181 ttcctcctgc taaaaagaag attgcctgca attcgatttg ctcagctact tgttgtatct
    21241 ctttgatcgt tacgctctcc atacgtttca accattcttc atcagataat tttgtttccg
    21301 gcagccagct gtccaaaaaa gaagcttcga ttgccgcttg cggagagtct agagaaagca
    21361 gatattggtt tctcagcatt gcttttgtct gagacagttc caactctgtt atttcaccat
    21421 ttcgcaaact ttctaattgc tcgtgaatca gatgaagtac ttgatttcgg ttcttttcat
    21481 cgataccggt ttggacactc atgaatcctc ggaacgtatc cacgctacta gaggcataat
    21541 aagctagact ttctttctct ctgacattca tgaaaagttt agagtgagga aaaccgccaa
    21601 ataaaccgtt aaagaccatt agcgcaaaac gttctggttc atcgtaatac acatttgtct
    21661 gataagctag gttcagcttt gcttgaacga ttggctcacg tacttgtcgt tccttcacga
    21721 tattcacttg aggttgggta taaaacagat ctggataaac tgtttcccgc gactcaaatg
    21781 gtaattgccc aactgcttca gctgctttgt ccggatcaac atcgccgaca acaaaaatgt
    21841 cgatttgatc atttgccatc atttcttgat agtaggacgc tagcgaacta gcagtgatct
    21901 tgtccaaatc ttcgactacg cccaaccctg ggatcttttg ttccgggctg ttttcaaaat
    21961 aaagttcttg caaggcatag gaagcaaaag tttgtttatc ttccttcaaa ctttccaaat
    22021 atagacgcag attgttcttt tccaagtcaa acgtcaactg atcaaactga tgatttttaa
    22081 tatttggata aaataaaacc tcttttaaaa aatctaccgc ttgagaaaaa aggtttggat
    22141 cattgacata ttcaccattc acaaaactga tccctgcgtt tagccaatgt aaatttcctt
    22201 tttttctaac tgataatcca aagcttgcgc cataaagctc tgcaagttgc gcacttaact
    22261 ttgtctgatc cgaataattc aaactatttg tttccaacat gctgctgaga agtgaacgtt
    22321 tcgtgatgac ttcttgttgc agacgcgcag taaaacggat aaaaatccgg atcgttttgt
    22381 attgttttgt tggtaagaca tgaagattca cgccttctgc caattgatac accatattta
    22441 tatgctcctt ttcacagttg attgcccgct tactctttta atgggtcaac ctgccttttt
    22501 tacttattcc tctgcttgaa atttataagt tcaggcaatt cgccgttttt tctaaatgat
    22561 gttacacttt atagtatagc atagatgttt tttatcaaaa gaaacagacg gctaaaagcg
    22621 tatattgttt ttttattttt tatttttcga tatactaaag acaaataaaa gaagggaggg
    22681 tcattcatga tcaaagaatt caaagagttc atcatgcgtg gagatgtctt ggatcttgct
    22741 gtcggcgttg ttattggtag tgcgtttaca agcatcgtaa accaagtggt gcaaggcttg
    22801 atcacaccac taataggttg gtttgtcgca atgatcacag gtacttctga tttagaagga
    22861 gcactttcaa ttcttgactg gtcgccgaca aaaggcgtca catttgcgtt tggtaacgtt
    22921 attagtgcta tcattacatt tctgatcact ggttttgtct tattcttagt tgtcaaagcg
    22981 gcaaaccgtg cgaagaattt acgctccaaa gaagagcaag tggaagtgga agaaactcct
    23041 actgcagaag attatcttag tgacatacgc gatcttcttg ccaaacaagt ggcagaagac
    23101 aatacacatc cagtagagca aacaaatctt tccgatactt ctaaataata aagaatctaa
    23161 aaaaaaagaa agccgatttt cagtgtcggc tttctttttt tagattctta acttgctact
    23221 tgttcctttt tcatgtcgat atgtggaata tcatcttcca aatacacatc tgagattgcc
    23281 ttgaatccaa acgactcata aaaagcttgt aggtattctt gtgcgccgat cacgattggt
    23341 ttttctggaa aatttttctg gatcatgtta atcgtttctt gaacaatctt tcttcctaaa
    23401 ccatctttac ggttgtcctg atgaacaagg acacgtccaa aatggatact ctccgcttct
    23461 tcatatactc gcgtgtaggc aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa
    23521 gcttctccat caatgtcatc aacttcttga taaggacact gttgttccac aacaaaaact
    23581 gcgattcgtt ctttgacgat cgcgtaaaac tctgctgttg acaattcttc aaatttcttt
    23641 acctgatatt ccactaagca aacgccccct gcttttttat tcttagcata ccgattcgca
    23701 cacaaaaaag caaacgaacc ttacgaatct caggctcgtt tgctttttaa actgcttcag
    23761 ttaaaaattc gatctcaact gtccgagtaa gtacatctga atagctgtaa gatactcgtt
    23821 caaaagaatt ttcttcagga tccaaatcaa caacgaatac tgaaggatag gtttctgtta
    23881 aaacgccgcg gcgttctgtt tgacgctttc ttcctgtctg agcaaccaac atgattgggc
    23941 tgccgatacg accttctaaa tctttcttga ttgaagctaa agttgttggc atacagttca
    24001 cctctactat caactatacc acatattttt aaaattttca agtttaccat aaaaaaaaaa
    24061 gaaatgcaat aaaaataaat aagaagctca ttaagatttt tctaaattat tcctcattat
    24121 aaaatttcaa gtgttttttt attttgagtt ttactctccc gtaagcgctc attacttgct
    24181 tgtgatccat tgctaaacat tcagccatgt ctacaaaatc aacgccttgc atataataat
    24241 gaaaaatttt agcttctatt tttgaaaact ggatttcgca attttcgatt gcttctttta
    24301 ataaaagatg ctgaattgct aaaggttctt gcaatgtgat ctctgctaaa aagtctggtc
    24361 cttctgtctc tattttttgg tcgattgaga cttcatcttt ttgatcacga cgcttcagcg
    24421 cgttctgctt cctcaacaga gaatagattt gattgcttat aacgctttta taataacttc
    24481 caaagctaac acctttgctt ttttcgtaag ttctcaatgc ctgatataaa gcgatccttg
    24541 cttcttgttc ccaatcctca gaaacataat ttcttaaata ataggtgttt ttcaaacgta
    24601 gtaaaagtgg aaaatatttt tgatacaggg cagcaaatgc ttcttcgtca ccattacgtg
    24661 cagcgcgcaa taactcctca gacataaaat aattcctcca taaattcgtt tatggaggaa
    24721 tcttatcatc aatgattcat cgaactgatt agaacgatat gttatttatt ttttctcatt
    24781 aaatcgtcca atttttggga caattttttt aactgctctt cattccatgg agaattacgt
    24841 cggaaatcaa taaaatgcat atctttattt tcttgggcta ttgttttttc cgtttttttt
    24901 atcgtcttat ataattcata agcagaagtt cgtaatgctc cttgggaaaa gatcatccac
    24961 tgttctgcta aatcgcttgt cgccactgtc acttgtgtca aacggtcatt cagctgacca
    25021 gcgattctct cgatataact gtcagccgtt tcgtcttctt tcgtaaaaac aacatccaac
    25081 tggtatttct tataattttg ttggatgccc ggaactagtt gggcatcgaa gacaacgatg
    25141 acttccattc cttcatattt cgcataattt gataaacgat gtaaaagagc ttctcttgca
    25201 tccgccattt tgtcctgctt tttaagctgg acaagttccg gccacgaacc aatcatgttg
    25261 tagccgtcta caatcaacag ctgttttttc atggtctact ccccctgacg ttgggaaaaa
    25321 gcctgataca taagcaaacc agcagcaaca ctggcattta agctctggac gtgcccggtc
    25381 atcgggatcg tcagcaattc atccatttct tttttcaatc ctggactcat tcctttgcct
    25441 tcattaccaa tgactaaagc aattgatcct ttggcattcc atttttggaa gtttgtgccg
    25501 gtcatatctg tgccaaatac ccaaaatcct gtttctttga gctgttgcac agcttgtacc
    25561 aaattcgtca cgcgtgcaac agggacatgt tcgactgccc cagtggaagt ctttgttacg
    25621 ataggtgtga ttccaactgc tcggtgtttt ggtatgatca caccatcgac gccacttgcg
    25681 tctgctgtcc gtaaaatcga cccaaaattg tgtggatctt cgatgctatc caaaatcaaa
    25741 aagaaggggt cttctttttt ctcttttgtt cgttcaatca gttggtcaag ggataaatat
    25801 tcataaggtg taatcgcaag aacgactcct tgatgcacgc cgtgatcgct caaagtttct
    25861 aatttttgtt taggaaccca tttgactggc acagcttgtt cacgtgcgat ttctttcagt
    25921 tcttctactt tttctcctcg tgaatcttct tgaagaaaga gtttattccc tcgcccttgt
    25981 tgtagcgctt ctactgcggc atggtgccca aagacgaaat tatcttctaa tgcttcttca
    26041 ttttgatcag aaactgactg atttcgtcca gtttgctttt tatttgaata tctattttgt
    26101 tttttgtctt caaatccacg tttcgattga tgtttgtttg ttctttcttt acccattttt
    26161 ttcaccaact ttctgaatac accagcggat caattcttcc atccgctcgg tttgttttgt
    26221 caaatgcaga tagcccatga gcgcttcgaa ccctgttgcg acccgatacg tcgtaacatc
    26281 agcatttttt gcgctcgtat ggcttttagc atttcttcct ctgcgataat aaagttcttc
    26341 ttcttctgtc aaaagatgtt cttccagcat ttgctgaatc aaaaaggctt gtgcattcgc
    26401 agaaacataa ttcgttgccg cacgatgcag cttattgggt tttgtttctc cttgatcaat
    26461 caggtggtca cgaacataga tttcgtaaat cgcatctcca acataagcca aggctaaacc
    26521 gttcaattgt gtgtaatctc tcatgcttct cttctccatc gggttccttg cggtgtatct
    26581 tctaataaga tccctttttc tttcaacaaa tcacgaattt cgtcgcttcg gacaaaatca
    26641 cgattgcttc ttgcttgatt tcgttcttcg atcaattgtt caatctcttc atctaataac
    26701 tcttctgctg taaataaaat accgaagatc gaaagccatt gcgagaataa agcttctcct
    26761 tgtgccaaga cagcggcaga aactttcggc tggtctagat aagtgttcag ccacttagca
    26821 agctcataga ccactgtgat cccatttgct gcattaaaat catcgtccat ttcttcgatg
    26881 aatcgctgtt ctaaattttc taacaaagcg attttatccg catcatctgc caaattctct
    26941 gaggcagttt cttggcggaa tttcaaattg tcaaacgtat tcttcagtcg ttgtaaattg
    27001 gtggctgctt ctttcatcgt cgtttcactg tagcggatcg gacgacggta ttgtgtggtc
    27061 gccatgaaga aacgcaagac ttgtggatca acgtttttga tcatttcatg tacagtgatg
    27121 aaattcccta gagatttact cattttttca tcgtcttcgc caatcgttac atagccattg
    27181 tgcatccagt aattagcgaa ttttttccct gttttcgctt cactttgtgc gatttcattt
    27241 tcatgatgag gaaattctaa atcttgcccg ccaccatgga tatcgatcgt ttctcctaaa
    27301 tgttttgttg ccataacgga acattcaata tgccaaccgg gacgtccttt gccccatggt
    27361 gaatcccaag aaatctcgcc aggttttgct tgtttccata aagcgaaatc taatgggtct
    27421 tctttttgct gctgttcttc ccctgtacgc tgacttgctc cgacttccag ttcatcgatg
    27481 gattggtcac ttaatttccc ataattttca aatttacggg tacggtaata cacatcacct
    27541 gaagattcat aagcatagcc tttttcaacc aacacttgaa taaatgctaa aatatctggc
    27601 atatgatcca tgactcttgg atgcagtgta gcaggttcga cattcaacgc tttcgtatct
    27661 tcttcaaatg ctcggatgaa acgatcagca acttctggtg ctgtaatacc taattcgttt
    27721 gctgtacgga tgattttatc gtctacatcc gtgaaattcg atacgaaatc gactttatag
    27781 cctcgatatt caaaatagcg tcgaattgta tcgaaagcaa tggtgctacg agcatttccg
    27841 atatggatat aattatacac agtcggtccg cagacataca taccgacatg tcccttttgt
    27901 atagggatga attcttcttt ttttctggtc aatgtattgt agatttttat cattctgatc
    27961 tcctcacttt ttctcctttg attcgaacga cttttgctgg tactcctacc gctgtagcgt
    28021 cgtctggaac atctttcaac acaacggcac ctgcaccgat tttggcgttt tttccgatcg
    28081 ttaccggtcc taatatctgc gcattcgcag agagcatggc tccttgttta acagttgggt
    28141 gtcttttacc cgtttctttt cctgtgccgc ctaatgttac gccatggaat aaaatcacat
    28201 cgtcctcgat ttctgcggtt tcgccaatga cgattcccat tccatggtcg atgaacacgc
    28261 cagttccaat cgttgctcct ggatggatct caacaccagt taggaaacgc cagaattgag
    28321 cattgatctt cgccaataaa aagagccggt gctgatataa aaaatgagag aaacgatgcc
    28381 aaaacaaggc atgaaaacca gggtatgtca aaacgatctc agctgtcgaa cgtgcagccg
    28441 gatcgtttct ctttgctgca tcaatcgctc gggtaaacca actcatgact ttccactcct
    28501 ttcgtaagaa ttttatgtag gaaatcatct tcttataaag aaaagaagcg ccttttaaat
    28561 aagtttcctt atcaaaagac gctttccgcg tggttccact tttcttcata atcaatgatt
    28621 atctctgaca gctaacgcct gttatacgtt cttggctact ttgtttccta taaaacagtt
    28681 tttcctgttt tctgtgtttc gccaagacca ctcccagagg catttcggca accattcatt
    28741 gataccttgc accacccggt atctctcttg taatgatcag ctgcttactt ttttctgttc
    28801 atcgtgttta gttcagtact tgatttaaat gtgctaatga tttttcacga ccaagcaatt
    28861 cgatcgtttc aggcaattct ggaccatgca tttgtcctga tacagctaca cggattggca
    28921 tgaaaagatt ttttcctttt acgccagttt ctttttgaac agctttgatg gcagctttga
    28981 cagttggagc atctactact tccatttgtt ctagctgtgc tttgaatgcg tggagaacag
    29041 taggaactgt ttcaccagca agtacttctt ttgctgcttc attcaataca ggatgttcat
    29101 tgaaaaatag cgtagacaga tcaacgattt ctgcagcata gctcatttga ggttgataca
    29161 ggctaacgat cttcttcacc cattcgattg tcttttcatc tgggttttct gctacacgtc
    29221 catcagcgat tagataaggc agacacatgt cagtaaatgc atctagatcc attgctttga
    29281 tgtaatggtt gtttacccat tgcagttttt tcgcatcgaa agctgcgggt gatttactta
    29341 ggcgttttgg atcgaacatt ttgatcaatt cttggtgttc gaagatttcg tcttcgccca
    29401 caggtgacca gcccaataat gcaataaagt tgaacattgc atctggtaga tagcctaatt
    29461 cgcgatattg ttcgataaat tgcaagattg attcgtcacg tttgcttagt tttttacctg
    29521 tttcactgtt gatgatcaat gtcatatgtc cgaaggttgg tggtgtccag ccaaatgctt
    29581 catagatcat cagttgtttt ggtgtattgg caatatggtc atctccacgt aaaacgtgag
    29641 tgattttcat caaatgatca tcgaccgcaa cagcaaaatt ataagttggc ataccatcgc
    29701 gtttttggat gacaaagtcg ccaccgatat tatcggattc gaacgaaata gcgcctttca
    29761 ccatatctgt aaatgcgtat tctgtattgc gaggcacacg gaaacgtaca acagattcta
    29821 gaccttgtgc ttctttttct gcttgttctt caggtgtcaa gttcgcacat gttcctgcgt
    29881 aatgaggcat ttctcctctt gcgcgttgtg cttcccgttc tgcttctaat tcttcttctg
    29941 tgcaatagca tttgtatgct cgattgctag caagcagctg atcaatcagc ggttgataga
    30001 tttctttacg ttcagattga cggtaagggc cgtattctcc aggattttca ggagattcat
    30061 cccaattcat tcccaaccaa gcaaggtttt ccaactggct tttttcgcca tcttcgatat
    30121 tacgtttttg gtcagtatcc tcaatacgaa taataaagtc tccatcattg tggcgggcaa
    30181 acaaataatt gaataatgca gttcttgcat ttccaatatg caggtgccca gttggacttg
    30241 gtgcatagcg tacgcgtact ttcgtcattt tcttcctctt ctttcaaact agactttgac
    30301 taacagcaaa aattccgtca gttacggctt ctcaaatatc atttatttaa agccactcag
    30361 cacaaatttt accttttcta aggtaaatag taaagccaac tggttgaatt tccttaccag
    30421 ttggctgaat ttctttttta ttttgattgt ttagtctttt tctctgtctc ttttttattg
    30481 tcgattcctc gtcctgcatg tgcaggttta gcaaagatca tgcgtccagc agctgtttga
    30541 agggcacttg ttacaacgac ttcgatacgt tcattcatgt aatgctgtcc atcttctacg
    30601 acaaccatcg tgccgtcatc aaggtaggct actccttgtt ggcgttctgt cccggctttt
    30661 actactagaa cattcatcgt ttcaccaggg atgacgactg gttttacggc atttgccaac
    30721 gcattgatat taagaacagg tacattttgg aattcagaga ctttattcaa gttatagtca
    30781 ttggttacaa tgacaccatc taaaagctta gccaatttga tcaatttgct gtctacttca
    30841 ctgatatctt caaagtctcc atcgtacatt tcgacagata taccctcttc tttttgcaag
    30901 gcatttagga tatctaatcc gcgacgacca cgcacacgtt tcaaactatc ccctgagtcc
    30961 gcgatgtatt gtagttcgta aagaacaaaa ttagggatca tcaaggttcc ttctaagaat
    31021 ccagtctttg taatatcata aatccgaccg tcaatgatca cgcttgtatc caagatttta
    31081 tatttatgga aattttctcc tgattttcgt tctaacagtt gactctcaac ttgactagct
    31141 gtttcttgct cgattttttt gcttcgtgaa ccaaaaactt tcttccattc ttcgattcgt
    31201 gtcgttccca ttctcaatcc cagatagcct aagatgatca tcagtaaaat cggcaataca
    31261 ctattgacga atggaatttc catattgtaa agaggggtcg agatcacaac acctaaagtc
    31321 aatccgatga tcgcaccgat cgcaccgaag agaagataag tcaagctgac ctcacttagt
    31381 ttttgttcta cttgtttgat aatagctgag atatgtttgg ccaacagcaa agataataaa
    31441 aagaaaataa gtgcgcctaa caggctgttg gtaaatgtat tattcagcca tccgtttgac
    31501 tgttgaccga ttgcctgcca agctgagggt aaaaatgaaa tacccaagct tgcgccgaca
    31561 atgatcatca gcaaagtaat gacacgtttt tgcatcgttt ttcctccgtt tcgacaatca
    31621 tttattagtt agcggaacac acgcttcaac gtttcagcca aagttgctac tccaactatc
    31681 tcgattcctt ttggcgcttc ccagttacct aagttgtttt taggtaggta tacttttgtg
    31741 aagccgagtt tttggacttc tttgactcgt tgttcaatat gactgacacg tctgatttct
    31801 ccagtcagtc caatttcacc gatgaaacat tccgttggct gtgttccttt ttctttatag
    31861 cttgaagcaa tactgacagc gatagctaaa tcaatcgctg gttcatttaa ttttacccca
    31921 cctgcagctt ttaagtaagc atcttgattc tgaagaagca acccagctct tttttccagc
    31981 actgccatga tcagagatac ccgattgaaa tcaagacctg ttgttgttcg tttggcattt
    32041 ccgaacatag taggtgtgac taatgcttgg atctctacca aaattggacg tgtcccttcc
    32101 attgccacaa cgatagcaga gccagttgca ccgtctaggc gttcttctaa gaaaatctgt
    32161 gaaggattag cgacttcttc caatccatgc tcatgcattt caaaaatccc aatctcattc
    32221 gttgagccaa atcggttttt tactgctcgc aaaatccgaa aactatgatg ttgttcacct
    32281 tcaaaatata acaccgtatc caccatatgt tctaacattc ttgggccggc gatcgagcct
    32341 tctttggtaa catgaccgac gataaaaatg gctatcccat ttgttttagc ggttttgagt
    32401 agttctgcag tggtttctct tacttggctg acacttcctg ccacacttgt gatgtctggc
    32461 tgcgtcatcg tttggatgga atcaatgata acataatcag gggagatatg ttcgatggca
    32521 cgactgattt cgttcatatc tgtttcagcg tatagataaa attctgtgtt gatacttccg
    32581 aggcgttccg cgcgtaattt tatctgctct gcgctttctt cgccggatac atataagact
    32641 tttccgccta tggctgctaa ttgctgagaa acttgtaaca gcaaagtgga tttcccgata
    32701 cctggatcgc caccaatcaa gaccatagac cctggcacca cacctccgcc taacacacgg
    32761 ttcagttctt ctaattttgt tttgatccga ggttcttttt tagggacgac gtctgcgatt
    32821 tttgttggtt ttgctttttc tcctgtcaaa cttgtccgag ttctgcggtc cgttgtatct
    32881 tgttcgattt cttcgaccat cgtattccat tttccgcaat ttgggcagcg tcccaagaac
    32941 ttaggcgaca catacccgca attttggcaa acaaattgta cttttgattt ttttgccatt
    33001 gtaccgcctc cttttatcct aatcatgaga cattaaatca agcttaagta tctttaacgt
    33061 tttatttact ggaagaacca aatccgcctt gacgttgagc agtcgtttga tcttgatctg
    33121 cttttaaaaa cggcaaaaag atcccttggc cgatacgctc gccttttttg atcactacat
    33181 ccttgaaacc aaagttggtg aattggaaca tgatatggcc ttcatttgcc tcattattgt
    33241 aataatcact gtcaatcaca cctactccgt ttgtcaacac taaaaaccgc ttcaacggat
    33301 tgcttgaacg gttagcaagc tgcaaaaatt catcttctcc catgtaagct ttgattccag
    33361 taggcacgag ggttggtttc aattgttttt cattttctga tacatctttt ttcaatccat
    33421 ctgcgactgt ttccaacccc tttttccagc ctgctttcca gatacttgga atcacgacct
    33481 cttctgctgc ttcaaaatca tagccagcag catgatgtgt agcgcgctgc ggtagattga
    33541 tattttgatc tttatattta gaaataactt caaatcctct ttttctcacc ggttttcctc
    33601 cctaaataaa ctactcctat tctaacatat ccgaggctgt aaaagacttt ctttacaaat
    33661 tcttcaagaa attctgctat cttaaagtaa accattaatt cgttcttttt ccaaacgact
    33721 gcaagttgta caaaaaaact attttttaca tgactttact cgtcaaaaca agtagaatag
    33781 aacgaaatta atgagtggat atttgattga ggtgtcacat gcgtaaagaa attaaaaatg
    33841 caaaacgaat tattgtcaaa gtaggaacaa gtacattgat ttatcccaat ggaaatatca
    33901 atcttgcggc aatcgatcag ttagcttttg tcctttctga tcttagaaat cggggaaagg
    33961 aagtgatctt agtctcatca ggagctgtag gcgttggtat gcataaatta catttgtcag
    34021 aacgtccgac tttgattcct gaacagcaag ctgcggcggc tgttggtcaa gccgaactga
    34081 tgaatatcta taatcaaaga ttcagtgtat ataatcaaca gacggcacag atcctcgtga
    34141 caagagacgt ggtagattat cctgaaagtc gtaaaaatgt gaggaataca ttggaacagc
    34201 ttttgattat ggacacgatc cctatcgtga acgaaaacga tactgtagca gtggatgaat
    34261 tagatcatct gacaaaattt ggcgataatg atcagctgtc agccatcatg gcaaaacttg
    34321 tcgcggcaga ccttttgatc gtcctttctg atatcgacgg gttttattca gataatccga
    34381 ctacaaatcc cgaggctcag atgtatcgct atatcgataa aatcgatgaa aagattctgg
    34441 cacaagcagg tggtgctggt agcacatttg gaacaggcgg tatgcagagt aaattaaaag
    34501 ccgcttctag gatatttgaa gcaaacagcg caatgatttt agcaaatgga aaaaatccgg
    34561 caattatttt tgatattctt gcaggaaaag aagtaggtac tttatttaag gaggacttga
    34621 aatgactgac ttgatgcaat taggacaaca ggcaaaagat agcgcgcaac aactagcact
    34681 tatgcctaca aaccgcaaaa atgagctact tgttcaaatg gcacaaacca tcaaaaagaa
    34741 tcaacaagcg atcattgaag caaatcataa agacttagaa aaagcagtcg aaaacaatat
    34801 cagtgaaacg atgcaagacc gcttgcagct gacacctgag cgaatcaatg ccatggctac
    34861 agagatcgaa aaaattgctt cgttagacga tcctattgga aaagtagatg agatgtggac
    34921 gaacaccgac ggattgagaa tcggcaaaaa gcgcgtgcca ctaggcgtga tcggcatcat
    34981 ttacgaatct cgtccaaatg tcacgacaga tgcagctagt ctagcattca aatcagggaa
    35041 tgccgttatc cttcgcggtg gcaaagaagc attcttctcg aatcaattat tagtccaatt
    35101 attgcagcaa gtactggtca gtgctggcga atctccttat gccatccagt ttgtcgatga
    35161 tacttctcat gaaactgcag gacagttgat gcgattgaca gaatacttag atgtgctgat
    35221 tcctcgaggc ggagcaaaat taatccaacg agtcaaagaa caggcaacgg tacctatcat
    35281 tgaaacaggt acaggaaata atcacgtata tatcgataaa gaagcacagt tatctatggc
    35341 agtgaacatc atcgtcaatg caaaagcttc ccgtccatct gtctgtaacg cggcagaaac
    35401 attgctgatc cattctgaga tcgcaccttt tttcttgcca gctattgaaa aagaactggt
    35461 agaacacggg gtctctttac gtgcggatgc tcgtgcgtta gaatatctag agacggctgc
    35521 tttagcagaa gacacagatt gggatactga atatctagat tatatccttg ctgtcaaagt
    35581 ggtcgattct ttggatgaag caattgcaca tatcaatcgg cataatacta aacattcaga
    35641 aacgatcgtg acagatagtt atgcggctag tcaacgtttc ttaaatgaag tggacgcggc
    35701 ggctgtctat gtcaatgcct ccactcgttt tacagacgga tcggtatttg gttttggtgc
    35761 agagattggt atctctactc aaaagttgca tgcccgtggt ccaatggggt taaatgaatt
    35821 aacttccaca aaatatatca tttatggcga tggacaaatc cgcgaataat gtttcgtgtg
    35881 aaacattatt cccgcctttt gccttttttc tgtaaaggtt ctatccaact agaaaggtgt
    35941 caatatgttt gaaattttaa tgattttttt tatttacctt atttctctga atatcgctgc
    36001 ttttctggga gtcggtattt tatccctttt ctttcaattc aaaaaacgaa gcatagggag
    36061 tcaacgggaa aaatggtcgc agtattttga caaaatcggg caaaaagggc tggttacacg
    36121 cttgcacatt agttacatgg ttgcattatg cttattagcg acaatcaatt actattcttt
    36181 tttcgatcat tcaatcgctt atacgattac gctactgatt gctgggattt ttcacttatc
    36241 ttataaatat cagctgaata aaaaccactt gaatcggact ttccgctaaa aaatatccgt
    36301 acgataagta gagtgccttg aagatcggca aaaaactgcc ggacttttta gaggctgtga
    36361 caaagatttt gtcacagcct ctttttccat ataaacggcg ttcgcctacc agctgcctgg
    36421 taaatctgtt tcctcttcgt taagaagcga aaatccgtca aatgtttcgc catcataata
    36481 acgaagcagc tcatccatgg acaagttcat cgttttttct ttttcttctt ccgctgattc
    36541 agccagtagc tgttcccgat acacctgata cataccagct attttttgct gcatcgcttt
    36601 tctagtttca ccttccgcat tgattattgg caattctttg atatagccac gataaatcaa
    36661 ccgatcatct gaaaattcaa tgggtaattc aacgagtgtc aacccgactt cctgaaaata
    36721 ttcttccagc atctattatc cccccattct ttttcttaaa tgaattttag cttacctact
    36781 gaaaattttc cagtttcatt attttgagaa aaaaagggaa tgtgacaaaa atcgtgtcac
    36841 atcccctttt tccgaataaa tggtgttctt ctgccaactc ctcaatcata agtcacaata
    36901 ttcaacaaac atgagaaact gttttttgaa attgttcctt gtgattcaga gttaaacgac
    36961 agtctcacaa cctcttggta aacggcgttc aatcagctga ccctcggtat tagctcaaaa
    37021 aacggtaaaa tatgagaagc tatttttccg ttttgtttct ctaatactta ggataaaacg
    37081 ctgattttac aacctctgtt aaacggtgtt caatcagctg tccttcggta ttagctcaaa
    37141 aaacggcaaa atatgaggaa ctatttttcc gtttgtttct ctaatactca ggacaaaacg
    37201 ctgattttac aacctcctga taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    37261 aaccaaacga aaaatatgag aagctatttt tgtttggctg ttcttatgac ttgatgcagg
    37321 acagcttttt cacaacctct gatttcttat tcttcttcat ttgtagcaaa ataattacgg
    37381 tgataaatga tacaacatac actgataaga acaatggcac ctgcaaaata aaacgcatag
    37441 ctttcaccaa tatgttcacc aatcacacct gcaataaatg gtgctaaagc tccaccgaaa
    37501 aagcggacaa aactatagct ggacgaagca acgtttcgtt gcagacctgg tatttccata
    37561 gcaatcgtcg tcaacagtgt attgataatt ccttggaaaa atcctgctaa cacaatgctg
    37621 atcggtacaa ggactggcat agaagttcca gctcctaata acagcaaaca aataatgaat
    37681 aagacaaatg ctgctaaaat tgtccgataa gtcgtaaact tccgctcaag agccggtgca
    37741 acgaaaatcg aagcaatagc cagaagcaca ccccagccaa aaaatacaaa accgacttct
    37801 aattcagaat agccaatcaa taaaaatggc gcatacgcaa gtaaagtaaa gaatccgaaa
    37861 ttatataaca atgcgattag tccaactgaa cgaagtttat gattgcttaa cgctttgaca
    37921 ccttcccaaa ggcctacttt tctctttggt ttctcagttt caggtaaaag taccgctagg
    37981 gctataaaac cgcaaaacat caaaaatgct accccaaaaa atggataacg ccatgaacca
    38041 ctgcccaaga ccccaccgac tagagggcca acagccattc cgcagccaag tgctgcttca
    38101 tacatcatga ttgctttttc tgtgttacca actaaaatac tgacgatcgt tgctaaagca
    38161 gtagagacaa ataaagcatt cccgacaccc cagccggcac gtaacccaat cagcattccg
    38221 atattcccag ataatccgcc aagaccagcg aatacaacga tgataaacaa tcctgtcatc
    38281 aatgtttttt tcaaaccgat ccgactagat aagaaacctg taaatagcat caccacacca
    38341 gtgactaaca tgtaactagt aaataacagg gtcgtttcag ctggcgtagc gtttaactgt
    38401 gcagcaatcg tttttaagat tgggtctact agacccactc ccataaaagc gatcatacag
    38461 gtagctgcta caactagcgc tgaacttttc ccctttgttt cttcatttgt ttgttcttgt
    38521 atatctattt gattctccaa tatttccatc cctttctctc tattagcatc atacatgatt
    38581 tattttacat gtcaatatgc acatgtaaaa cgtggaaact ttactgtgat ataataaaga
    38641 aaataagacg agaggtagga tttttatgaa tactttagcc tatatattat tatgcgtgct
    38701 tttatccaaa ccacgttctg ggtatgaatt gaagcagctg atcacaattt tttgggaagc
    38761 ccatcacagt cagatctata caacgcttgc caagttagac cagcagggtt atgtgaccgt
    38821 tttggacaaa gacgagcact ctcaaaaaaa gatctaccac ttgactgatg aaggacgtgc
    38881 tcttgtagag gaatggatca aagaggaaac tcccgctcct acgcaaaaag atgaatttct
    38941 agcaaaaatc tatgcttttt cgacgctcga taaaacaaca gctactggac tgctattcac
    39001 tagagaacaa cagctgaaca aaatcatgca aaaaaatcaa aaaaaattga atgacctctc
    39061 gctagacaag aaacaagaat ttggacgtta tgttgtgatc cagcgcagaa ttatgctttg
    39121 tcagcaggaa ctccaatggt gtcagcaagt aagagaacaa atgcagcagt tttttgagta
    39181 agggataaac agaacctttc tagaaatatc tattgaagag caaattatct tcaataaaag
    39241 agcaatacag ttggaaaaga aaaagcgatc cgaacgacag gccaaaacct atcgttcgga
    39301 tcactatttt tctagaactg cattcaagag actatgacaa aaagcatgcc aaggcctttt
    39361 ttcaaataac agtgttccat cattcattac tttgaattgc ttttagcaca cttcttgaaa
    39421 gcgggccaac gatgatcaac tgcaacggta aagcggcaat cacattcatt ttccaagcat
    39481 tcatataagc agagaataat tgatcaggta ttccttgttc catgatcaca ccgaataaag
    39541 acatacatgt caccattcct aaaaccatca agcatgaaat agttaagatc atttggacct
    39601 tgctttctgg atttactggt agtttaaagg caattttttt cgccagcaca ccgatgatga
    39661 agacatccaa aatgaaagcc accacgaatc caggcactag accaatcaac aaacttgata
    39721 aatgaagatc ttgatgcagc cataaattat acgcgctcat ccccaagacc atcaaaaaac
    39781 acatcattgt tgtaaaaatt attccttctt ttttatttgt aggcaatctg taccactcct
    39841 ttaaatttgt cacgaaaatc atcgtagcac atttaaaaat ttctcgtaag tacttttttt
    39901 attttctgcc cttttatttc ataaacttca tcagccgtac gctcgacaaa gtaagaatca
    39961 tgagaagtaa acaaaatcgt tcccggatat atttgcatta gcttctctaa agcttcgatc
    40021 gttgccatat caataaaatt agtcggttca tctaacagca acacattact tggcttagta
    40081 aaaagcaaag caatcgtcag acgggtcgct tctcccccac ttaatgtaca taacggtttc
    40141 gtcaaagttt cctgagcaaa ccctaagtga ttcagcaaac tacggacaag cgattctgaa
    40201 aactctgttt gtctcatgac aaaacgaatg atggattctt cagatgtcat ttgataaccc
    40261 ttttgctgat atacttgaaa gcttactttc ggagagagaa ggattccttc tccttgtttg
    40321 cggatatgtt ctaataaggt tgtcttacct gaaccatttt caccgacaat cgcgattttt
    40381 ttacctaaag aaaactgaaa atcaccattt accagtaatg ttctcccact tctttccaat
    40441 tggacatttt gtcccatgat gggatagcgg ctgtggagtt caagagattt tggtactgga
    40501 aaacggattt gtttcatctg ctgcggcttg gttgtttctc ctattttttg gagtcttctc
    40561 tctaatgttt tcgcattctt ttggatggct ttttgtacac tgtctttttg tttagaggaa
    40621 gacaaccgat ccagtctgat ttgttgcttt ttctttttac tggacacttt ttccaacttt
    40681 tccgcttgtt ttttcttttc ttggatcgat ttttccaatc gttttttctc tttcatgatc
    40741 gactctgctt ctcgtagctg agtctttttc tccaattcct tttgagtgag ataggcacta
    40801 taattccccg aaaactctcg gatttctccg tcttttactt cccagatttt cgatgctagc
    40861 tcatctagaa aatagcgatc atgactaaca aaaaccagcg taccataata ataacgaagc
    40921 tcttcaatca gtcgatcaat actttttcta tctagatggg ttgttggctc gtctagaagc
    40981 atcccttgtt cataagaaga aagaagacgt atcaactcga tctttcgttc ttcccctccg
    41041 ctccatttcc gctctgaagt ttcttccagt cttaactgga aatgactggc taattccaaa
    41101 tttaggtcct ttgcttcagc gagataggtt aattgaggca ggtagttgaa ggtgatctct
    41161 gtttggattt tcccatgatc gggaaaaagt tcaccggcta ttagtttgag caaagtcgat
    41221 ttaccgacac cattttctcc tatgatccct atacgatctt gctgatagac acgaagagaa
    41281 tcaatagata gttctagctg gttgccaaag ctgacttgta gatttgttat atttactgct
    41341 aaattttcca tatgttttac actcctattt catgtaaaac aacaattatc gatactaaaa
    41401 aagagcagaa aaatctgttt gcaaaaaaac tgcatcacat cttttcctac tctgaacgaa
    41461 ataactatga ctaaatcacc aaatctgtca tagctttcac ctattctact gtcgttcgaa
    41521 aaaatggaca tactccatcc atgaaattgt tgtttataca tgtcttttga atttatttag
    41581 ttgttattca aaagttcgaa acgtaatttc atcgatgcag tcatttttcc gacctccttt
    41641 tttattaggt taccacacac gattcaaaaa aagcaatagc tactgaatag taactgaaaa
    41701 ccttttattc gaacatagtg atcttttctg gtgctaaacg aacagactcg taaccttctt
    41761 ctaccgcttt tcctattgat aaaataataa ctggaacata acggtcttta tccaaaccaa
    41821 atgcctctgc caactgatct gcttcgaatc caccaattgg atttgtttca tagccatgat
    41881 cacgcgcaac taacatgaat tgcatagccg ctaagctaga atcgactttg actacatcgt
    41941 tcatttcttc tcttgaaaaa ctcttgtaat aaggaatgat cgcagctaat tgttgatctc
    42001 tgacttcttt tggcatcttc cctgattcgt aagcttggtt gtagatttct tcgccatatt
    42061 cgtaacattg catatcgcca aaaatcatga ccattgcaga agaagtatca ttttgtctcg
    42121 tattgaaacg gatcaaagga cgcaagattt cttttgcttc ctcgctttca gcgaccacaa
    42181 agcgccatgg tttcatattg acagaagaag gtgcagtcgt cgcttctttg atcatttcca
    42241 gcatttcttc gtgtgggatt ttgtatgttt catcatatac gcgaacggat cttctaccag
    42301 tcataattgt tgaaaaatca tttttttgtt ccattattat taacctcttt cgtttctgtt
    42361 gttatattgc aaagttgggt attcaattta tctaaaagtg ttagaaattg tttcatttct
    42421 acatcgctga aagccgggga tagcacggaa cctgtatctt tatggtccat ttcacaatgc
    42481 ttgattttct tctgggcttt ttctgttagc gctacataca cttcccggtt gttctgagga
    42541 tttctctgac gcgaaacata gccatttgct tccaatacct tcagatgacg agtaactgct
    42601 ccttgatcaa tctgcaactt ttcttgcaaa ctgctttgta gcaatggatt ttcttctttc
    42661 agtctcatca tgatctcgta tctagtaaga cttataccga tattttcttc aaagatctgg
    42721 acgatttttt gatcggctaa tttgatttga tataaaaggt gactaatctc ttctaaagac
    42781 ataatttcct cctttctaat aattgactcg tcaacgattg acttgtcaat aatagaataa
    42841 aaaaagaaaa aagtcaaaga atcagctgtg taataaaaaa aacgctctca atagaaaagt
    42901 atagagaaaa gaaatttttt gtagaatgaa cggaaaaaat gataaagaaa gcacaacgct
    42961 aaaaccaaga ttattgattc ttatcaaact atagaagata gctcttctgc agtagataat
    43021 tttacaccgc caaaaatttc cgaaatcgcc ttttgcaaga atcccgtcga atattagaaa
    43081 ttggatcagt atgcttaaac agtaataaaa aagttatgac aagatgttgt cacaaccttt
    43141 tttgagtaaa cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa
    43201 catgaaaatc tgtttttgtt tggctgttct tactacttga tgcagggcag ctttttcact
    43261 acctcttttt aataaccata aaatcctatt ccttcatatc tccagcccat cggtgttagc
    43321 caatctcttt ccttagcact caaggtatag tgatgggtac cagttttttc atttggatta
    43381 aatagtcgat aaattggctt ggaaccacca gaataaaaac ttagaccttc ataacgccaa
    43441 ccgatttttg ttaaagagtt gatctcagat tgtgccattg tgtaatgatg gtctccactg
    43501 taaggattgt ataaacggta gacaggttgt ccgctgtttg gtgcttgcca agcaatccct
    43561 tcatattccc agcctacagt tggcagataa tttttttctc cggcattcaa tgtatataaa
    43621 tgttctcctg tatgacggtt atacagacga tacatcggag aagttgaagt tggtgctgta
    43681 tcatactgtg tcagattata ggtttcgatt acattgttca atgtagaacc ataattaggc
    43741 gcagtggcat aacgacctgt tagccaagcc gtagcatctt tgtaagagtt cgtattgctt
    43801 ttccatgcac cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt
    43861 acgttatctt gcaatgattc tttgtacgaa ggatattttc taaaagcggc attcaccgta
    43921 atccattgcc cattcacata ttccgaagta ggcatcgtaa cagattggcc attatagctt
    43981 cctttaatcc caaatagatt gtaattaggg gcagaagcca aagtactgtt cccccagcca
    44041 ctttcaacga tggcttgagc aatcatgact gatgcatata agtcattggc acttgccaaa
    44101 ctttgtgcag ttgaggcaat actatcaatg aacgcttgtt gagaattagc agatgagcga
    44161 gcataaaaag catagccagt atagttttcg aattcagaca aatctgcttg atgggtatgt
    44221 gtcaatgttt gcccatctgt caattggttc gtgatttctt ctgcattctc tggattgttc
    44281 tgagcttctg ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt
    44341 tcttcggttg aaattgtatt ttctgagaca gtagctgact gcaaagtact ttctgtatcg
    44401 ttttcaattc ctgtttctga agaactcgat gtctgagttg cgacagtttc tgtcgattct
    44461 tcagctagca ctgttgtgtt ggcaactgct agatttccta aaagaataac gctaaataat
    44521 aaattctttg ctttcatctt ttttctcctt tgaattatga aacttttcct aaataacagt
    44581 gtaactaaaa aagtaaaagc aagtcaatgt ccatatttaa ataacatttg tttcttttta
    44641 ataaatttgc tttttctgca taaaaagacg taaatgaaga atattcttct ccattcacgc
    44701 cttaaattga tggtcgtttg aataactatt taacaagtaa ttctctctgg atattatccg
    44761 ttatggatta tacttcctgt gtcgtcggac ggataatcat gttgctgaca gacatccggt
    44821 ctggcgtttc tatcgcaaat gctacagcag aagctatatc ttcagatgtt aatccccatt
    44881 ctttttgcgc ttcatgaagt tcaagagcag cctttttgtc gctgatggtt tgatataatt
    44941 cagtttgaac agctcctgga gagatgatcg tagatttgat gttattttgc cgttgctctt
    45001 gacgtaatcc ttccatgatt gcacggacag caaattttgt tccgcaataa acggcggaat
    45061 caggataaac aacatgtccc gccacagaat cagttgaaat gatatgccca gatttttgtt
    45121 tttccatgat tggaagtacg gcggaaattc catttaacac acccatgatg ttgatatcaa
    45181 gcatattttg ccattcatca cggtgacctt caactaatgg agcggttggc atgattcctg
    45241 cattattgta caatacatcg attcgaccga acttttccat cgctaaatcg ataactgctt
    45301 ggacttgtgc aaaatctctt acatctgctg tttgaatata tagctcagct tctggtaaag
    45361 attcttttat tgcttttagg cgatcttctc ggcgtgcagc tataactagt ttagcccctt
    45421 tttctgctaa tagcttcgtt gttgcttcac cgattccgct tgaagcgccc atgatgacaa
    45481 tcactttttc tgataaatca ttcattctat tctcctcctg ttttgaaaaa tctcttgctt
    45541 atactcacta tctcttattt catcttactg ttcaaataat ccgttcgaca ggtttgacga
    45601 ttttggcagg gattcctgca acgatggtat tgtctgggac atctttcgta acagttgcat
    45661 tcgctgcaac aatggcattc tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc
    45721 cgatccaagc atttttcttg atgtggattg gctttactga caagcctctt cgttcttttg
    45781 gatcggttag atggttcacc gtgatgaggt tgacacgagg accaatcaat actgaatctt
    45841 ctaaacagat gccacctaag tcaacgaaca tcacttgacg attgataaag acatttttcc
    45901 ctatactaat atgtttgccg aaatctgtat aaaaaggcag cagaacagtc accgtatcat
    45961 ctactttgtc actaatgata ttttctagcc gtttgcggat atctgctggt tcatggtacc
    46021 cagtattcag cgctgctaaa ggctcttcgt tttccgcttg gatctgatgg atcacttcaa
    46081 agagcggatc cgttactcga atctctttat tgacgatttg tttttcgatg aggcttgtca
    46141 ttttgctccc tccttttatc cttactttat aacggacggc ttatgatgtc taatacttat
    46201 aaaacatact taactattcc ttttaactat acttagtggt atgctttttt aaaaacagga
    46261 gggaaaaaat ggatattcgt atactgacgt attttttagc agtagctaga gaaaaaaata
    46321 tcagccgagc agcaaaatct ctgcatatca cgcaacctac tctctcaaaa caactgaaag
    46381 atttagaaga agaacttggt gtgacacttt tcactcgagg ctcccgagag atacagttga
    46441 cggattctgg tcgatatctt tattcaaaag gaaaagaaat cttgtcgttg gtagaaaaaa
    46501 cggcgaacaa tttaacaaat gacgcaatca tctctggaga aatctacatt ggcggaggcg
    46561 aaactaaagc aatctctttc atttctgata gccttcattc gttgatcaag gcgcatcctg
    46621 atatccagtt tcatttatat agcgggaatg cagatgatat catagacaaa ctggatagcg
    46681 gattacttga ttttggactc gtaatcgaac cgacaaacaa acaaaaatac cagtcatttc
    46741 gactgccagc ccaagatcgt tggggacttt tgatacatcg gaatcatcct ttagcaaagc
    46801 aaacagtcat ccatccgcag gaactgaaaa ctgttccttt gatcatttcc cggcaaacgt
    46861 cagtggacag tcaactagct gaatggctag gtttcagcat agatcagtta tcgatcgtcg
    46921 gaacatataa cttattgtac aatgcttctt tgatggtcga taacgggact tttggtgcat
    46981 tagctctaga tgggatcatc aatacttatg ggacggaaaa agtattcatc ccactcgatc
    47041 cgccattaca agcttcactg aatctcattt ggaaaaaaga ccagcctctt tctcctgctg
    47101 ctcgtaaatt tttagatgtc ctaacggaga aatacagtta agtagagttt ttttccttcg
    47161 ctttcagctt tttaacaacg aataagttaa gcaaaagatc caatgattaa ataacataag
    47221 tttcttaatt tgttccttcc ttttatttct gataattcca gtaaattact ataattataa
    47281 aaaaagaatt gacaaaacat aactgtctat ataaaattga ttacgttaac aataattcgt
    47341 ttcatgtgac tagatcatac taggcatgaa aaatctctga ggaacatggt ttccctagga
    47401 ggtttttcgt gtctattttt gtttggccgc aatcaactgg tctagcaaac gtgaaaacca
    47461 aatcttcaaa aagggtgcgc gcatgaaaat caaaaagatc tttaatcaaa atgcagtatt
    47521 ggtagatgac ggtggacagg aaaaagtagc tattggaaaa gggattggat ttgacagaaa
    47581 aagaaatgat ttgatttttg atcgtgatgt cgaacggata tttgtcatgg aaccagaagg
    47641 tcaaatcaag ttgcaaaatt tacttaatca gattgatgaa cgctttttat ttgcagcaga
    47701 aacgatcatt gatcatgcgg aaactgtttt gatggaaaag ttgaatgaac atgtattgat
    47761 cgctttgacc gaccacattg ctttttctgc ggagaacatt aataacggta tcgtcatccg
    47821 caacaaactt ttaagagaaa tcgaagtgct ttacagtgat gaattcagta ttgctcaatg
    47881 ggcagtagag tatctgacaa aagagcttgg gattccatat acttatgacg aagcaggata
    47941 tatcgcaatc catattcaca gcggacgaag cggtcggaac aataaccatc gcagtatccg
    48001 tgaagtgacg attgtatctg acatcatcca tttagtggaa tcagaattgg aaacagatat
    48061 ccattcagaa ccattttcac tgaattattc aagactggtg aatcatttac gactgcttct
    48121 acaacgaacc catgcgcaac aatatgctgt tttagacacg gaaatcgtag aaatggtcaa
    48181 acgaaaatac ccagaaagct ataagatttc taaaaaaatc cgtgtgttgc tgaccaaaga
    48241 ataccagctc gcgatcacga aagaagaact gggttacttg gccatccata tcgagcgttt
    48301 aagaagcgca atcgtacaaa caaatgttaa caatcatgag gaggaaaaaa attgaaagca
    48361 tatctacaac gaatgggacg ttcccttcaa cttccagttg cggtcttacc tgctgctgct
    48421 ttactagtag ggatcggaaa ctggtgggca tcttatagta acgacattgt cgctcatttt
    48481 ttacaagctg gcggtaacgc agttttaggt caattacctt tgctgtttgc cgttgggtta
    48541 gcactaggga tgtcagtgga caaatcaggt gctgctgcct tggcaggttt ggtcgcattt
    48601 gaagttccag taaatgtttt aaaaactgat tcagtagcta ctttattgaa tatcaaacca
    48661 gaatcagttg atccatcttt tgctcagatc agtaacgtat ttattggtat tattgccgga
    48721 ttgattgcgg cagctcttta taatcgtttc cacgaagcaa aattaccgat ggctttatct
    48781 ttctttagcg gcaagcgtct agtgccaatc ttggctgcct ttgtcatgtt gctgatcagt
    48841 gcagtgctac tgctcgtatg gcctccggtt tactctgctt tagtttcttt tgctaaattc
    48901 attttgagtt taggctgggg tggtgcaggt ctttatggct tcttcaaccg tttactgatc
    48961 cctacaggat tgcatcaagc aatgaactcc gtgttctggt ttgacgtagc aggaatcaat
    49021 gatattggta aatttttggc ttctgaagga acaaaaggcg taacaggaat gtatcaagct
    49081 ggatttttcc caatcatgat gttcgggctt ccagcaggag catatgctat ttacagaaat
    49141 gcacgtccag aaaacaaagc aaaaacagct tccttgatgt tagcagcagc ttttgcttca
    49201 ttctttactg gtgtaacaga accacttgaa ttctcattta tgttcgttgc ttggccatta
    49261 tatgtcttgc atgcaatttt cactgggatt tctttagcag tgagtgcatt cttccattgg
    49321 acagcaggat ttgcctttag tgccggattt gtcgattact tcctatcttt gaaaaatccg
    49381 attgcgaatc agccattgat gttgatcgtc caaggattag taacagcagt gatttactac
    49441 tttggcttta actttgctat caaaaaattc catctgatga ctcctggccg cgaggaagca
    49501 gatctttcag atgacgatac tgcaacgact aattcagaca ataaatatgc ggcacaagca
    49561 agcaaaatct acgctgcatt aggcggagcc gacaatgtaa cttcgatcga taactgtact
    49621 acacgccttc gtttgcaagt caaagataca ggcctgatcg atcaaaataa aatcaaagcc
    49681 actggcgtac ctggtatgaa gatcatcgac ggaaaaaatg cgcaagtcat cgtaggtaca
    49741 gaagtacaat tcgttgcaga tgagatggca aaactacatg gtggtgctgc aacacgacct
    49801 gcacaaacaa acaccgtggt taaaacagaa acatttgccg gtggaacgat cgaacaagat
    49861 atttacgcta tcgcaaatgg gaaactgatt ccaatcactg aagtttcaga tgatgtattc
    49921 gcggaaaaaa tgatgggtga cggctatgct gtattaccag aaaatggaga aatcttctct
    49981 ccaatcgctg gtacgatcac aaacattttc ccaacgaaac atgctgtcgg aatccaaaca
    50041 gatgctggta tcgaagtctt gcttcatatg ggcatcaaca ctgttgactt aaaaggcgaa
    50101 ccattcactt tatatgtaaa agaaggtcaa aaagtcgcaa gaggtcaatt gattgcttta
    50161 gttgatcttg ccgctattca atctgcaggc aaaaacacgg atatggtcgt tgtattcaca
    50221 aatggcgaca aagtccaaag cttagaaatc gaacccgcac gtgatgtaaa agcaaatgat
    50281 aaaatcggct ctgtttccaa caaagcctga gccatcgtct tccccagact tttttatcga
    50341 taaacaaaca ggagctgaga caaaaatctt gtctcagctc ctttttccga ataaaaacgg
    50401 cgttctcctg ccaactccgc ggtaataagt ccagccaaac gtaacatatg aagagctatt
    50461 ttttatttga ctcttcttat tacttgatgc agaacagctt ttttacaacc tctgataaac
    50521 ggtgttcaaa aaccagttta tcggtatgaa gtcaaattta tgcaaaaatg tggaaagcca
    50581 ttttcacaaa atttttcttc atactcaaaa ctaatcggct ttttcacaac ctctttatac
    50641 attattcctg atccaatatt tcttttaatg ctgcttcaag agaagttaat ggttttccca
    50701 atactttttc taaatcatca gaggaaatag ctaactgatc ctttttaata tcatgttgga
    50761 tcgctaaaaa catgtcagct gcttcttgag ggaatccggc ttcttttaga ttttcgacaa
    50821 acgctgcatc acttgcttca ataatttcta actttttacc tgtcgcacgc tctaatgcct
    50881 tggctaattc ttcatagtca gttagattgc cggataattc taagatttct ggagagtcac
    50941 tttctactag tacttttgct gcagcttctg catattcacg tttcaatgcc caacctgttt
    51001 ttccatttcc tgcagcataa acgaattgac cagtttttaa agcctgccca atgataggta
    51061 attcattctc tacataccaa ttattccgta aaaatgtatg ggaaatacct gaatccttaa
    51121 tcattttttc agtgaattgg tgatctggcg ctaatacact gatcgagtga tcggcatctg
    51181 caaaacttgt ataagcgata taagataccc cagctgcttg tgctgccttc actacattct
    51241 tatgttcggc ttgacgattt cctggaacgc cagagacaaa aagcaatcga tctattcctt
    51301 tcaacgcttc tttcattgag tctaaatcat cataatcacc aatacgtata ttaaatccag
    51361 cttctttcag tgcttctcct ttttcttcac tacgtactag tacatagatt tcattttgtg
    51421 gaaccatctt ttttaagaat tccagcgcat aaccaccaaa acctccagtt gctcctgtta
    51481 ctaaataatg catatttgcc actccctatt ctttttattt gttaagattt acagctgtac
    51541 atttataaat tacaccttta tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg
    51601 aaaggatgac aactgatgaa gtattctctg caatttagtg atgcaatcca tgtacttgcc
    51661 tatattgaaa tgtttcaaga tacccgatca ctttctagtg agatgattgc cagtagtatc
    51721 gaaacaaatg cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc
    51781 tttacaaaaa ccggaaaacc gcagccttcc cttgccaaaa gtccagatca gatttcttta
    51841 ctagatgtct atcgcagcat cgaaggtaat acacagctta tccaagtcga tccaaaaaca
    51901 aatccaaatt gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag ctacgctcgg
    51961 ctgcagaaaa aggcagaaga agaaatggct aagatcacac ttgatagtct gatacataaa
    52021 atagccgttt tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga gagattttta
    52081 taattctctc tactgtgcct gcatttttct gatcggaatc gtctacataa attcttttat
    52141 tctccggggg acaaaagcct tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc
    52201 gctgaagtca tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg cattagttct
    52261 gaaaaatgca aaatatggaa agctatttcc catttttcct ctctaatgct caggtcaaag
    52321 cgctactttc acgacctcta tattatcatg gtttattgac taccggtatc cagatttctc
    52381 ccatatattt cccgttggta actcccattt cgactacagc atttggaccc ccaacatagg
    52441 cgtaatcttc tacttctctt aaaacatgcc caaaggccgt attcgtcaaa tcatttctta
    52501 attcttcagg cgattctgct aaagcgatta caacaagata cgttcctttt gggaatggga
    52561 tgattcgggt agtgtttgga acatcctgac tcgtttgaac accgacatag tacatcattt
    52621 tcccgttata gggttcgttg actgtaaaga aataatgatt ttcagcagct tgtttcaatg
    52681 catctattgt tccattaccg ataacttttt gctcaaatgc ttgtttctct gcagccaaac
    52741 cgacaaaatc cgtattgcta ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg
    52801 gtttttcttc gatcctatac tttgccattt ctcccaccct ttctattata ttaagttcat
    52861 cttacataaa attaaaggga aatgacacga ttacgctctt ttttctaggg atatttttat
    52921 tttttaacta tttttttgct ttcactgcaa tccaaatctc actgtaaaca ttactgaaat
    52981 cctctcccca ttttgtaaaa gagatctcgg gagcttcgac taactcataa tctgaagaag
    53041 gaagccattc tgcatagatt ttcccccaag tatcttgtaa agtttgggga aatggccctt
    53101 tattaggaaa aatggcccaa gtgtgttctt ctatagcaag catttctaaa tcagcgtacg
    53161 gattttcttt tgtcgtagca aagccaatca tgtgcatcaa actgcccttt tcttccaagc
    53221 gggctccatc aaatccataa gaaacgttga ttacttgatt tggatataaa tctcctaact
    53281 tatgcatctc ttccctttga gtctccgtga tcgtctgcgc taacttttgt atttcttgat
    53341 tgactccttc aaatttgaca ggaacttttt tcgccacacc tgccacataa aactgttctt
    53401 tcttttcgat acgaaattcc atcgattttc ctcctctgac atcaataaaa aatgtcaagc
    53461 gtggaaaaga tttttgtatg ccgtatctgc tgatttctga aggcagatat cctgaccaat
    53521 cacgaaaagc tcttgaaaaa ccttcgactg tttcatatcc atatctaaac gccgtttctg
    53581 tcactcttga tccatctagg agatcgcgat tagccaaagc caatctacgg tttttgatat
    53641 agtccattag cgacatccct gcaataaacg taaagatgcg ttttaattga tattcagaga
    53701 ttcctgtatt ttcggctatt attttatagg aaagtccttc tgtcaaatgt tcttcgatgt
    53761 aatcgatcaa ttggttcaga tctttaagca tatttatctt cctccttgct tgccttttta
    53821 tcatattatc aaagaaagtc aattacttga tgaatcagga accaatttag tcagatgtag
    53881 aaaatgttgt atggatctaa agaaaattct caagcagcgt ttcgtaacgg ataattggta
    53941 ttatctgcct atctatgttt tacctttaga aaaataaatt ttggggaaga cacattctaa
    54001 gtaaacaaag cggtaaaaat atcttttatt cccttagtta atccgcttac tacctactgt
    54061 acaaagcaaa ttgttgttca aactgctgat cagaacgctg cttggatttt tctttgcgat
    54121 acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat cacttcattt
    54181 ttgtcagata aataaaa
//
LOCUS       DANKAZ010000006        54024 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000006 DANKAZ010000000
VERSION     DANKAZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            271..591
                     /locus_tag="PWY67_000336"
     CDS             271..591
                     /locus_tag="PWY67_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL2056649.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            745..1650
                     /locus_tag="PWY67_000337"
     CDS             745..1650
                     /locus_tag="PWY67_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2056650.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1647..2420
                     /locus_tag="PWY67_000338"
     CDS             1647..2420
                     /locus_tag="PWY67_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2056651.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2601..4025)
                     /locus_tag="PWY67_000339"
     CDS             complement(2601..4025)
                     /locus_tag="PWY67_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2056652.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4236..4682
                     /gene="nrdI"
                     /locus_tag="PWY67_000340"
     CDS             4236..4682
                     /gene="nrdI"
                     /locus_tag="PWY67_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL2056653.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4747..6729)
                     /locus_tag="PWY67_000341"
     CDS             complement(4747..6729)
                     /locus_tag="PWY67_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2056654.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6743..7639)
                     /gene="murQ"
                     /locus_tag="PWY67_000342"
     CDS             complement(6743..7639)
                     /gene="murQ"
                     /locus_tag="PWY67_000342"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2056655.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7642..8703)
                     /locus_tag="PWY67_000343"
     CDS             complement(7642..8703)
                     /locus_tag="PWY67_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2056656.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8914..9480)
                     /locus_tag="PWY67_000344"
     CDS             complement(8914..9480)
                     /locus_tag="PWY67_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2056657.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(9508..11010)
                     /gene="eat(A)"
                     /locus_tag="PWY67_000345"
     CDS             complement(9508..11010)
                     /gene="eat(A)"
                     /locus_tag="PWY67_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2056658.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11268..12389)
                     /locus_tag="PWY67_000346"
     CDS             complement(11268..12389)
                     /locus_tag="PWY67_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2056659.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12515..13456
                     /locus_tag="PWY67_000347"
     CDS             12515..13456
                     /locus_tag="PWY67_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2056660.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13606..14502)
                     /locus_tag="PWY67_000348"
     CDS             complement(13606..14502)
                     /locus_tag="PWY67_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2056661.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14771..16111
                     /locus_tag="PWY67_000349"
     CDS             14771..16111
                     /locus_tag="PWY67_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2056662.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16329..16772
                     /locus_tag="PWY67_000350"
     CDS             16329..16772
                     /locus_tag="PWY67_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL2056663.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17276..18694)
                     /gene="nhaC"
                     /locus_tag="PWY67_000351"
     CDS             complement(17276..18694)
                     /gene="nhaC"
                     /locus_tag="PWY67_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2056664.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19097..20320)
                     /locus_tag="PWY67_000352"
     CDS             complement(19097..20320)
                     /locus_tag="PWY67_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL2056665.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20351..21190)
                     /locus_tag="PWY67_000353"
     CDS             complement(20351..21190)
                     /locus_tag="PWY67_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2056666.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21207..21887)
                     /locus_tag="PWY67_000354"
     CDS             complement(21207..21887)
                     /locus_tag="PWY67_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2056667.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21889..22962)
                     /locus_tag="PWY67_000355"
     CDS             complement(21889..22962)
                     /locus_tag="PWY67_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2056668.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23018..23256)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23299..23532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23609..24280)
                     /locus_tag="PWY67_000356"
     CDS             complement(23609..24280)
                     /locus_tag="PWY67_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2056669.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24367..27045)
                     /gene="mgtA"
                     /locus_tag="PWY67_000357"
     CDS             complement(24367..27045)
                     /gene="mgtA"
                     /locus_tag="PWY67_000357"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL2056670.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27704..28141
                     /gene="ndk"
                     /locus_tag="PWY67_000358"
     CDS             27704..28141
                     /gene="ndk"
                     /locus_tag="PWY67_000358"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL2056671.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28163..29029)
                     /locus_tag="PWY67_000359"
     CDS             complement(28163..29029)
                     /locus_tag="PWY67_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2056672.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29189..31282)
                     /locus_tag="PWY67_000360"
     CDS             complement(29189..31282)
                     /locus_tag="PWY67_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2056673.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32031..32654)
                     /locus_tag="PWY67_000361"
     CDS             complement(32031..32654)
                     /locus_tag="PWY67_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056674.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32813..34210)
                     /locus_tag="PWY67_000362"
     CDS             complement(32813..34210)
                     /locus_tag="PWY67_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2056675.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34242..35213)
                     /locus_tag="PWY67_000363"
     CDS             complement(34242..35213)
                     /locus_tag="PWY67_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2056676.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35587..36012
                     /locus_tag="PWY67_000364"
     CDS             35587..36012
                     /locus_tag="PWY67_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056677.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36500..37201)
                     /locus_tag="PWY67_000365"
     CDS             complement(36500..37201)
                     /locus_tag="PWY67_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2056678.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37217..37657)
                     /locus_tag="PWY67_000366"
     CDS             complement(37217..37657)
                     /locus_tag="PWY67_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2056679.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37657..38232)
                     /locus_tag="PWY67_000367"
     CDS             complement(37657..38232)
                     /locus_tag="PWY67_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL2056680.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(39080..39559)
                     /locus_tag="PWY67_000368"
                     /pseudo
     CDS             complement(39080..39559)
                     /locus_tag="PWY67_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(39718..40419)
                     /locus_tag="PWY67_000369"
     CDS             complement(39718..40419)
                     /locus_tag="PWY67_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2056681.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(40527..41936)
                     /locus_tag="PWY67_000370"
     CDS             complement(40527..41936)
                     /locus_tag="PWY67_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL2056682.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(41984..43849)
                     /locus_tag="PWY67_000371"
     CDS             complement(41984..43849)
                     /locus_tag="PWY67_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2056683.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(43970..44821)
                     /locus_tag="PWY67_000372"
     CDS             complement(43970..44821)
                     /locus_tag="PWY67_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2056684.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(45131..45844)
                     /locus_tag="PWY67_000373"
     CDS             complement(45131..45844)
                     /locus_tag="PWY67_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2056685.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(45912..46238)
                     /locus_tag="PWY67_000374"
     CDS             complement(45912..46238)
                     /locus_tag="PWY67_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2056686.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(46361..46588)
                     /locus_tag="PWY67_000375"
     CDS             complement(46361..46588)
                     /locus_tag="PWY67_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2056687.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(46910..48163)
                     /locus_tag="PWY67_000376"
     CDS             complement(46910..48163)
                     /locus_tag="PWY67_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2056688.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            48224..48964
                     /locus_tag="PWY67_000377"
     CDS             48224..48964
                     /locus_tag="PWY67_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2056689.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(49050..49808)
                     /locus_tag="PWY67_000378"
     CDS             complement(49050..49808)
                     /locus_tag="PWY67_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2056690.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(49805..50833)
                     /locus_tag="PWY67_000379"
                     /pseudo
     CDS             complement(49805..50833)
                     /locus_tag="PWY67_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(50853..54020)
                     /locus_tag="PWY67_000380"
     CDS             complement(50853..54020)
                     /locus_tag="PWY67_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056691.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
BASE COUNT        15480 a        11385 c         9343 g        17816 t
ORIGIN      
        1 tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag cattcatctt
       61 cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa gtatgagatt
      121 gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg ccaccgggta
      181 ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct ctttttgtgt
      241 atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat catagcagga
      301 attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc taaaatcaat
      361 tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc aaaggcaatc
      421 aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc agttggttcg
      481 gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg gttctttgtg
      541 gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta gacacttgga
      601 ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt ttatgaaaaa
      661 ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat gcttgtacta
      721 tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt atccaagaac
      781 tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg caaaatcgtt
      841 ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat tttaggactg
      901 atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc ctctagtcat
      961 aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc attcttaaca
     1021 ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat cgacactatc
     1081 gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta ctcgttggga
     1141 atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat cgttatttta
     1201 gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa aatcattacc
     1261 gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag cgagcttgaa
     1321 aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca atgtacaatg
     1381 gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa tgatctagct
     1441 gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa aatcaagctt
     1501 ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc acaaaatatc
     1561 catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt ggaaattctg
     1621 gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa ttatttaaat
     1681 tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg ctcataggaa
     1741 cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg actgctcagc
     1801 tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc acgatcattt
     1861 cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat tctcgaggag
     1921 aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta taccttttag
     1981 ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc ttcacggaga
     2041 aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg ggtagttata
     2101 tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc aatagttccg
     2161 gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata tctggtatcc
     2221 ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt atgttgaatc
     2281 tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt tctactaatc
     2341 aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga tatctcgtat
     2401 ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag acgttcagcc
     2461 ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc tgtgacaaca
     2521 tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc acaacctctc
     2581 tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa aaccattgca
     2641 atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat atgttgtaca
     2701 ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa gaaaagaaca
     2761 agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg aaaggcactt
     2821 gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag tttcatttgg
     2881 tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt gtaagcaaag
     2941 ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca taacgtcgtc
     3001 aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc tttaggcaaa
     3061 agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca tgagagccaa
     3121 acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca tttaccaacc
     3181 atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag ctgattcatc
     3241 gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa gccgaatatc
     3301 cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc tacgacagag
     3361 gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa tacttgactg
     3421 gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa gttatcaatg
     3481 aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa tggcactaat
     3541 ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg attcactagc
     3601 catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt ttgcccacct
     3661 tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa agctacgttg
     3721 cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa acggccaaaa
     3781 ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt attgatatta
     3841 ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact gaaaatagct
     3901 gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc tccaatcgct
     3961 gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg ttgctctgta
     4021 tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac aaaccttttt
     4081 tatttttact taattaataa aaactttaat agaaatataa ttctaccggc ttttttctca
     4141 ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac ttaaaaaaga
     4201 tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat atatcgattt
     4261 caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa atgcatgcag
     4321 aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt gattttacga
     4381 aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga aatggtttag
     4441 ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa catgaagaca
     4501 atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat aatcagtatt
     4561 gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca gattatgaat
     4621 tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa aacaataaat
     4681 aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc tattgtttct
     4741 atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac ctgttttttc
     4801 caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg atgtttcttt
     4861 gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag gtgtcacttg
     4921 ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg tgttgattcc
     4981 catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt gtttcgttgg
     5041 gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt ctggttctac
     5101 aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt tatctaaaga
     5161 aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg aagtttgagg
     5221 tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt cttctttttc
     5281 taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag cataagctgc
     5341 caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga tcaaagcggc
     5401 tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca ctgccccgcc
     5461 gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg ttactccgta
     5521 aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga tcatttctgt
     5581 taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca cttgtcctgc
     5641 accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa tcatttgcac
     5701 atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga ataatgtgcc
     5761 tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc caccgaccat
     5821 gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca gaccgatggc
     5881 aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta ctttatgcag
     5941 ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga tcccaccttg
     6001 tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg gattcatccc
     6061 tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac caaaaacttg
     6121 tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt tcaagatgtt
     6181 catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg tgactaagtt
     6241 tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag gaatcattgg
     6301 cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt tttgtttcga
     6361 tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc cagatgctga
     6421 tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca cgcctgccaa
     6481 atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga cttgaagctg
     6541 ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga catttacaag
     6601 atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc tttctacatt
     6661 gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag tcagttcgtt
     6721 ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt gcttcagcta
     6781 attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata gccagcttaa
     6841 cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct tctgtcgctt
     6901 gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg acatcgacca
     6961 tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc atattcaaga
     7021 taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat tctggacctg
     7081 catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta ttgcaagaaa
     7141 tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc acatatggtg
     7201 tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc agcttgatta
     7261 gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca gtcattgctt
     7321 cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat gtcggtacgc
     7381 attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata taaatcagac
     7441 gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata cttgtcagtt
     7501 ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga acggcttgag
     7561 aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt gttaggttct
     7621 ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat agttcatacc
     7681 agctttgatt tggttgatca atggtagctc ttctttgata actttagcta ctacattcac
     7741 tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat agcgcaaata
     7801 cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc ttgctgggat
     7861 aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg catcgtcttg
     7921 ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag gttccgtatg
     7981 gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt tttctgacag
     8041 acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta gagcgcctgc
     8101 aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata gtgggcctct
     8161 caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt tgagccattg
     8221 attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat aataattatg
     8281 ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga tatctttagg
     8341 cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg caatctgctg
     8401 attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca aaggcgttag
     8461 atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga ccatcaattc
     8521 tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat atgccgaaac
     8581 atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg atgccatttg
     8641 gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg aaaagccaaa
     8701 catattatcc ctctttttct gtatccgtct tcatttctat gactagagta tatatctaaa
     8761 gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa ggttgagttt
     8821 atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa gcagtgcctt
     8881 gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca attctgtatt
     8941 atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag cattgatcac
     9001 tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt cgatgcattc
     9061 tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat ttgggaatcg
     9121 tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca gtccatcata
     9181 atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc tgagacgatc
     9241 agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat
     9301 tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct gtcctttttc
     9361 agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag ttgtttctcc
     9421 gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc ccttaaccat
     9481 accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa tctcagtaga
     9541 tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt tcacgttcaa
     9601 gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg gttcatccca
     9661 tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt tccgttggcc
     9721 catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa gctttcggag
     9781 gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg ccaacgttcc
     9841 tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg gctggtattt
     9901 ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg atgactttcc
     9961 tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa aatgaattgg
    10021 ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa gtcgcttatg
    10081 gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt ttaatagttt
    10141 ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc gtttcatcgt
    10201 ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag tatcgatgaa
    10261 tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg ctttttcagc
    10321 tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag ccatttcaaa
    10381 ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat aaagttccag
    10441 ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc cccggtcatg
    10501 gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt gttttctacc
    10561 agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt catccacaaa
    10621 taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga attctctcca
    10681 taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc atatctcaaa
    10741 atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg tctgttgcgg
    10801 gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt tgttttgtag
    10861 aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta gtttccattg
    10921 tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt cgtagccgaa
    10981 tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa aagaccagtc
    11041 ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa agacccatac
    11101 atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct ccacaaggta
    11161 cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat ccctcacttt
    11221 gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta cttcgggagg
    11281 atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa tgtttgcctg
    11341 tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac aattgggaga
    11401 aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat tattggtttt
    11461 cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat cgtttgctga
    11521 tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt ttccaatcca
    11581 gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa aaaaagggct
    11641 ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac ctcccagaaa
    11701 tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag cgtcgtatct
    11761 attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt atatggattg
    11821 gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct tcgatcaaaa
    11881 tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa agaagcaccc
    11941 aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa agtgacaata
    12001 atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc ataactggaa
    12061 gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa tgcttgtgca
    12121 gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct gatttttcgc
    12181 tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag cgcgtaatag
    12241 attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt tcttccttta
    12301 cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc acttccttta
    12361 aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct taagtaagac
    12421 actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt ctatatacta
    12481 aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag ctggggccgg
    12541 agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg tcctcttgat
    12601 cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg ctgactttaa
    12661 cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca tcgaaagcaa
    12721 cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg atatgttttg
    12781 ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa atggattagg
    12841 tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg ggatcacgat
    12901 gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg gagaaatcga
    12961 actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag aagtctttca
    13021 agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt ggcgaaaagc
    13081 ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata ttgctgaact
    13141 aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat ttgcttctat
    13201 cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata ttgcttctac
    13261 ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc tgatcaaaaa
    13321 ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag gacaaaaata
    13381 tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa aagaaggaat
    13441 ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag ctggttctct
    13501 ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc cataagaatt
    13561 gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt tgttttcaac
    13621 agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga taaatcgttt
    13681 tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct taaatggtgt
    13741 atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa tattttcagg
    13801 acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct tttgaaaata
    13861 catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca gttccagaat
    13921 cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc caggttgttc
    13981 attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta taaattcctg
    14041 cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa atagatccgt
    14101 ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat tatgtttcag
    14161 cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg ccggttcatc
    14221 ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa aaaataacac
    14281 aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa atccataatc
    14341 taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa tgaattccga
    14401 ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca aaaaataaag
    14461 tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg cctcactttc
    14521 tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa aacgtttcct
    14581 atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc attcttctat
    14641 taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg ttatcattag
    14701 tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat ttaaaagaaa
    14761 gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac atatcaccat
    14821 gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg aagcgattgg
    14881 gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt tatatatcgt
    14941 gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg gattatctgg
    15001 tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg tctactggtc
    15061 tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt tgcagttgtg
    15121 gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa ctacgatcat
    15181 caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt ttgctaaaat
    15241 cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc aaatgttggg
    15301 cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta ctcctcatgg
    15361 tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg gttctgaact
    15421 gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta aagcaatcaa
    15481 aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc ttttgattct
    15541 ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg tatttgaaaa
    15601 aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg ctttgttctc
    15661 atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt tgaaggatcg
    15721 aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc aacgtgctgt
    15781 tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg tcggtgacaa
    15841 attatttggc tatctggcag gttccctttc gtatacagta ttattgatct ggatcattat
    15901 cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata aaggaatcag
    15961 cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca cgaactccat
    16021 tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt atcaaaaaaa
    16081 gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat tttatttatg
    16141 tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg caactccttt
    16201 ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc tctgtgcaaa
    16261 ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa aaaaggagtt
    16321 tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca acggtagtta
    16381 tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc agtcgtacac
    16441 gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg ttgttcatgt
    16501 ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt cttcttgttt
    16561 ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca gcttggatga
    16621 aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct gatttaaaag
    16681 atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa gagatcgaca
    16741 aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata aaaaaagagg
    16801 atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga acgccgtgaa
    16861 taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc
    16921 ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt gaacaccgtt
    16981 tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa atggaaaaat
    17041 agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat tgaacaccgt
    17101 ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat tttgtgaaat
    17161 ggctttccac atttttgtat aaatttggct taataccgag aaactggctt ttgaataccg
    17221 tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta tgtttttatt
    17281 gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt tttgagattc
    17341 cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg aaaaatacga
    17401 atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt ccggcaattc
    17461 cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta cctaatacag
    17521 ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc agaataacag
    17581 ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat gttgtaaaaa
    17641 tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt gagatcaagc
    17701 caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac acagtaggca
    17761 tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt ccagtatttg
    17821 aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt gggttttgaa
    17881 taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt gccggcacct
    17941 ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt gaaatagtaa
    18001 agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg gctttacttg
    18061 tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca gttgaccaca
    18121 tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct agatttgttg
    18181 attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata gctccaacaa
    18241 ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc ccaatcgttg
    18301 acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca acaaatacag
    18361 aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga gttggaatga
    18421 tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata aagattggga
    18481 tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc gggtatcttt
    18541 ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata ttaggtgaga
    18601 cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat acaagtgttg
    18661 cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt ttagttgttc
    18721 ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc gcgattcctc
    18781 ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc ggtaccacct
    18841 taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat ggactttcca
    18901 tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct gaacaaaaca
    18961 cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat aagatcattt
    19021 gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata actttcacac
    19081 acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct actatttctt
    19141 gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca agagctgtat
    19201 tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt ttagcagcag
    19261 ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc gtaggctgga
    19321 ttgttggtat gacttggctg acatttccta catcagaaga accgagactt gttctttgtt
    19381 ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag aataagtcat
    19441 caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca aactcaaaag
    19501 tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca tatactttat
    19561 ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa tctggaatga
    19621 cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct tttggcaatt
    19681 gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa gcatttactc
    19741 ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg tcgactggat
    19801 cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg cataacgcta
    19861 catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat ccgttctcgc
    19921 ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca tcaatgaatt
    19981 gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc ccgcatgcat
    20041 gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga ccgggtttat
    20101 tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt tctacccaaa
    20161 acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca tagttactta
    20221 cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa cgatattgga
    20281 ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc tcctatgcta
    20341 gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat ccttttgatg
    20401 ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt ttttgatacg
    20461 ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga cgtgcatggt
    20521 catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca tctacagcta
    20581 caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt tgggaagcat
    20641 ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca gtgggcgctt
    20701 gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga agagctctgc
    20761 cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata tcagctacat
    20821 ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg atcgatacta
    20881 aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg aaagaattca
    20941 aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta aatttcacgt
    21001 attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat acatctgtat
    21061 cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta tctgcttttc
    21121 cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca aaacgaataa
    21181 tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag ttgattactg
    21241 ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat cgttacatct
    21301 gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc tgcacctact
    21361 gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga gacccgaatg
    21421 ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg actggttccc
    21481 atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt ctcgatttga
    21541 cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt cgcaccaatc
    21601 gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat aatgaaaggg
    21661 atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg gttagctagt
    21721 aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat catcccgcca
    21781 atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac aaattcatcc
    21841 ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt atcttcctcc
    21901 ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta tttttacacc
    21961 agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag ctaaagtaac
    22021 gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata aaatattcgc
    22081 tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct gatttccaat
    22141 ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag aatgggcaat
    22201 gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt tggtcaattc
    22261 ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc ttccatcttc
    22321 cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag tgatcaggac
    22381 gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct gaagggtcgt
    22441 tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt cattcgccaa
    22501 agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag gataagcaga
    22561 tgctttttct tccaaaccga caagttccag tagttcattc actttttgac gacgttcctg
    22621 tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag ttcggctttc
    22681 cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc gcaattcttt
    22741 gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg ttggtttctg
    22801 cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac cgacgattcc
    22861 ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta tctctttttc
    22921 tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc ctccttatct
    22981 aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt ttttgcgtgt
    23041 taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg atcatactct
    23101 agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg aattgctcga
    23161 tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc tctgtcaaag
    23221 gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa gtccttttac
    23281 taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt ttcggaagtt
    23341 tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta acggtcgaac
    23401 agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc agcttttctt
    23461 acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta ctttcctttt
    23521 caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg aaaagtcaag
    23581 tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt aacatttcaa
    23641 taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt tcttcgacgg
    23701 tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct gctcgtgaag
    23761 cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct tctaatcctg
    23821 ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac acttcgtctc
    23881 cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata tctgttgctc
    23941 ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt aagtctgcca
    24001 atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt tgataaaaat
    24061 attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct gctttcacta
    24121 aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt tcttttggga
    24181 tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt ttttctgtgt
    24241 caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta atcctttcca
    24301 aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa gaaccagcct
    24361 tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag ctgtcacaag
    24421 cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag gaagtggcat
    24481 caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa taattcctaa
    24541 tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta ctttcggtgt
    24601 acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc atcctgcatg
    24661 gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac ttccgcccac
    24721 tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa atatcgaact
    24781 tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt tttttggctc
    24841 gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa catcatagat
    24901 cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga acgtacttgc
    24961 aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga tatatttcat
    25021 gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg tcaaatcttt
    25081 ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat ctacagaaat
    25141 accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta aaaatccgac
    25201 agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag gattcaattt
    25261 tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca tttccgagat
    25321 tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca ctgaacgtgt
    25381 aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt tcagagcttt
    25441 caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac caatcaaaac
    25501 catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg tcttttgtgc
    25561 gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa gtacggcttc
    25621 ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt tagaaatgtt
    25681 cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg gatcagcaac
    25741 cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt tttgataatt
    25801 gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat ctaacaaatt
    25861 tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac gatcatcttc
    25921 atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca atgtccccgt
    25981 tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca aatttttgat
    26041 cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg taacgatcat
    26101 tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa aagcttctag
    26161 ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca atgacataaa
    26221 tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt gaattggttt
    26281 ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag aaacaatgat
    26341 tccttccgca cttccactga tgacattact tcccataaag acaagatttt cgtaacttgt
    26401 ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat agctttcccc
    26461 tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca tatccgctgg
    26521 tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt ccataggaat
    26581 ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg atttcagttt
    26641 ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc tgacgataac
    26701 catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg ctaatacata
    26761 ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg gcgtgatgta
    26821 tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg cattttcacc
    26881 gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg atgcatggaa
    26941 tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt tttgtgtatc
    27001 tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt ctcgctgctt
    27061 ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct gagaacagat
    27121 agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt catccatttg
    27181 ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt tcgaatagtt
    27241 atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg cggcaacaaa
    27301 aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa aaagcttctc
    27361 agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct gcttttagct
    27421 accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc tctcgacgtt
    27481 tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat tcggttttcc
    27541 ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt ttttcatact
    27601 ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc tcattttcca
    27661 cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa gaacacttgt
    27721 tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc aacgttttga
    27781 agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg aattagcaaa
    27841 agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg attacatgac
    27901 ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa
    27961 gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag gagactacgc
    28021 gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg cggtaaaaga
    28081 gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag cggcgaagtg
    28141 atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc agttttttct
    28201 ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag aattcttggt
    28261 agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa taatctgcca
    28321 tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt ttcatatctt
    28381 cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa gtcaggttca
    28441 ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt cgcattcttt
    28501 ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa ggaaaatcag
    28561 ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat tcttcaatga
    28621 ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt ttttcgacca
    28681 tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc ctacggttat
    28741 tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc attcgtcttt
    28801 ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat tctgcaagtg
    28861 tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt ttcactgaat
    28921 ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat tgggactgtg
    28981 tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata atagttctcc
    29041 tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga aaaatacacg
    29101 aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat tagaatgttg
    29161 tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt tcaacgtctg
    29221 ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa aagacacttg
    29281 tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca tttttatagt
    29341 catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc aagagatttt
    29401 cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga tctttggaga
    29461 tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga ttattgtaag
    29521 cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga aaagcaacta
    29581 cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct ttatcatcga
    29641 ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat tcatgattga
    29701 ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc atctgtgcgt
    29761 tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa tctttattat
    29821 ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt tggtaaatac
    29881 cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa tgtgccgttc
    29941 gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg aaattcttgt
    30001 catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa aacggctgtt
    30061 gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat atcccataac
    30121 ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca taacctaagt
    30181 ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa acagcgcttg
    30241 tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg tctgacccaa
    30301 gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac atattttcag
    30361 catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg atcgttgctt
    30421 gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc ttcatattaa
    30481 tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct ttagcgatcc
    30541 caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa acttcatcaa
    30601 tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg attccgtcat
    30661 aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca aatgttcgct
    30721 gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa ataagccctg
    30781 aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg ataggtattt
    30841 tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc cagtagatga
    30901 catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac actttcgaaa
    30961 agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa ataatattca
    31021 agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac gctggctttg
    31081 gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt gggttgatcc
    31141 acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca tagtaggcaa
    31201 tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa cgattttggc
    31261 acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt ttcttatttt
    31321 gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt ttattctttc
    31381 taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag cgattaaact
    31441 aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa aagccagttt
    31501 ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct ccttatttct
    31561 tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg ccaactcctc
    31621 ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt gttccttgtg
    31681 gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgaccc
    31741 tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg gtgttcaaaa
    31801 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta ttttttgaaa
    31861 ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca
    31921 atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact attttgccgt
    31981 ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt tcattgtcca
    32041 taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg attttcttca
    32101 cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt cgactcgttt
    32161 ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca ttccgctaaa
    32221 cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac ttttccattt
    32281 ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa atgcttgtct
    32341 ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt aataaattga
    32401 ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta attggcagtt
    32461 attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc tactgattct
    32521 ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa
    32581 aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag aagaaacggt
    32641 ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta ttatatacaa
    32701 aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa cttctttttc
    32761 acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt tcttaagcct
    32821 caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg attgttcttt
    32881 ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat gcattggtcc
    32941 acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca ccttcttgaa
    33001 tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa tcatcgtgga
    33061 tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg ttttcagaaa
    33121 taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca tagattcctt
    33181 gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt tcataattgt
    33241 cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct tttgctttta
    33301 cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct ttgataaccg
    33361 tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc tcaatcaatt
    33421 cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag aacgcatctg
    33481 ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag cttggtgtca
    33541 gattcaagat gataccaata acaccacctt gctccatttc atggtaagat ttgatggcta
    33601 acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata tctgactctt
    33661 ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt tcattatgag
    33721 taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca tatttttcat
    33781 aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac atcggcatat
    33841 cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca tcaaaataac
    33901 gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt atcaaacggc
    33961 tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac tgtacatcct
    34021 cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca aagaactttt
    34081 gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg cctgaaatcc
    34141 ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct ggaaattgat
    34201 attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat tccatgtatg
    34261 atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat tcgttttaac
    34321 agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt gattttcgga
    34381 aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat atccttcccg
    34441 attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt gtaattgctg
    34501 acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata gccgcttttt
    34561 ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg gaccgcttct
    34621 ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc aataaatccg
    34681 atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact ttctcgatca
    34741 ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc gataactgga
    34801 atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa gaagacaatc
    34861 agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt ttctggctga
    34921 tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct ttcgatccct
    34981 gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc aatagagaag
    35041 cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa cgctgaaatc
    35101 gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa gtaacgggaa
    35161 actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc catacattct
    35221 cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat gaccgcattc
    35281 tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt agagcaactt
    35341 cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt aatatagcat
    35401 ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg attttttgcc
    35461 tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg tttttctctt
    35521 ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc aaatggggga
    35581 agtattttga agaaattaat cgtactattt attttgtctg cgggactttt gacattgact
    35641 ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga ttcttctgtc
    35701 ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt acgtaaagat
    35761 atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg gacacgcacg
    35821 tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt gctgacagat
    35881 atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa taaaaaagaa
    35941 acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga tggcaacatc
    36001 tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt ttctgctata
    36061 gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc ctgattattg
    36121 tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa gcttgagcat
    36181 atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat cttatcctgt
    36241 tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc actatggctg
    36301 atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag taaaacaaac
    36361 agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca aatatttgaa
    36421 tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt tttctttccc
    36481 gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg ttatccgtaa
    36541 aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc tgacggatca
    36601 ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc acttgtattt
    36661 taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac gtttcataat
    36721 aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt tttttgatac
    36781 gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc cgtttttttg
    36841 gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac tgctgtactt
    36901 cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta tcttcataga
    36961 tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct aaaaatcgct
    37021 tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa taaaagagcg
    37081 gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc tcttttgcaa
    37141 gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa atcactgaca
    37201 tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc aatgtttcaa
    37261 aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt tgtgtaatca
    37321 tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc aacatagttg
    37381 ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag tcaaggatag
    37441 caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact tcctgcgccc
    37501 atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag tagaaacttg
    37561 tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg atttgctcct
    37621 ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct tcaataataa
    37681 ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat tgttggaaaa
    37741 gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag tttctaataa
    37801 ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct tgtcgggcgt
    37861 cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac ctagacctcc
    37921 agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt catcgatcgt
    37981 ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa aaagcccatt
    38041 cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa tcactcccat
    38101 aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca tgtattttcc
    38161 taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac cgcaaaaaac
    38221 agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc tacacgtgtt
    38281 tttttaaaat aaaagactag attttgtagt tttttagtgg tatataggat agacgccact
    38341 atatatagta tttagaaaaa aatcattttt cagccatttt atagataaaa caaaaataaa
    38401 taacgtgttt tcatcgatat gaaatgactt acttatagaa gatactggga tttcgtccta
    38461 tatgccaata acgtgtatta ttttaaacaa caaatacttg tttcttttat taacgaaaaa
    38521 atcattttgt tctcgtgtta tgtttcactg ataaatgatg tttctctaaa taaaaaagcc
    38581 gacgaactaa acgatcaaaa tttgatcatt tttgttcatc agcttctcct aaaaatcttt
    38641 ttgttaacga taaatagctg gatttgtaaa aatatcactt gaatcgtcac tgctggaaga
    38701 aaattcagtg ataaccgcac ctgttgcgcc tgcctggaac tagtgtttga tattgggctc
    38761 gaacgtgtac tgatctctag catgaagaat aatttctttt tttactgtat aataactttc
    38821 tttccttttt ggaatagtac aggaaagttt ttttgtttcc tttccttcga cataaggtat
    38881 accagaccct ttcgacaacg aaaggttctc atttttcctt catcgtcatt tcaaggaggg
    38941 tgccgatgct ctggacagtt tcgatcgctc ctaaaccaaa atctgtatat tcgattcttt
    39001 ctaactctga ttctttcata ccattcctgt ctcaaggtac atctattgta cttttttctt
    39061 gtaatccatt ctgcctctcc taactaattg tcaaatcatc ccaagatatg tcttcttcct
    39121 ctttttcttc ttggatgatt tctgcattcg gatctactgg ttttttcaat aataaaatgg
    39181 ttattcctgt gacaaaagaa ccagctaaca atgcaccaat gaatgcccat ggtctggtca
    39241 tcgtagcgac tgcaaacatt cccgatgccg gaatacggct atctgcgccc caaaacatgc
    39301 ttaatccgcc acctactgtc ccaccgactc ccgttgctat gacattgcgt gccaagtcgt
    39361 tcaatacgat agggaaacat ccctctgtga tctgacagat ccccatagga aaagcggtct
    39421 tcaatgtatc gatttcttga cgattgagta tatttttacg aatgattttc cctaagaaat
    39481 aagcgatcgt caagccgaat ggggttacca tcgaagctaa aatcaataca gtgattggtt
    39541 catagattcc ttctgaaaat cgtgccacac ccccttatcc agaataaatg gtaatccaga
    39601 aacagccgtg tatctgcggt aataagccta accaaacgaa aaatatgaga agctattttt
    39661 gtttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt tttttattta
    39721 tgtttcataa tcaatatggt aaggatgcag ctccgtatgg tagaccgcct ccgaatattc
    39781 aatcatttcc ccaatttgcg tagacgattt ccgtatccgt ttgatgccga tctgtgcgtc
    39841 tgttttcaga attccttgtt cttcttctgt taaaaatact gccgagaaat catcagaaaa
    39901 tgagtcaata gtatatcctt ttttcgtcag caaacgatac aaagattctt tgccaaattc
    39961 ttctctgctg aattgcatca tttcttgtgg aagataatga acaaaataaa tataaggctg
    40021 attgttcaaa taatataagc gcttaaaaca aacagcttgc tcaccgaaaa aggaaaacaa
    40081 aggatcttcc ttctttatat ggaccaattg caaatccagc gtttctttcc ggacttcata
    40141 tcgggagttc tccaagattt gcgtaaagct tgccgctttg gataatcgat tgtaaggacg
    40201 attgcttaga accgtcgtcc cacgaccact tttctttgcg acatattcat ctgatgccag
    40261 cagttcgatt gcctttcgga cagtgatttt gctgacttga aacagttctt ctaattcact
    40321 ttctgtcggc aacatcgaac cgactggata ttttcctgag aaaatatctt ctttaatttt
    40381 atctgcgact tccaagtata gtacacgtgt ccttttcata acgtcaccct ttgctttact
    40441 attactaaaa gaatagcaca taaaaaataa aaaaagaaca caaatcacat gtgagaagtg
    40501 tgttcttttt cattcagctg ctttaattac aagtttacgc catttgaggc aattacttct
    40561 ttataccaat caaaagattt tttctttgat cgattcagtg tcccatttcc ttcatcgtct
    40621 agatccacat agataaagcc gtaacgttta ctcatttctc ctgttgatgc actgacaaga
    40681 tcgatacatc cccaaggcgt atagcccatc aaatctacac cgtctgctac tgcattagcc
    40741 atcgcttcga tatgacgacg taaataatcg atccgataat catcaataat cgtgccatcc
    40801 tcttccatat gatcaatggc tccaagaccg ttttcaacaa tgaataatgg tttctggtaa
    40861 cgattgtata attcattcaa tgcgattctc agcccttctg gatcgatctg ccagccccat
    40921 tcacttgcct caagaaacgg attttttact cctcccagca agttcccttc tgaagttgct
    40981 gcttgtggat cagtctcatt gattgccgta gacatataat aactaaatcc gatgtagtcc
    41041 actggatatt cttttagcaa ctggaagtct ttttccgttt gctctaattt ttccggatcg
    41101 actccatatt ttttatgggt acgcgctgtg tatgcagggt attctccacg tacttggaca
    41161 tccgtgcaga aaaaattgaa ttcttggttt tggatcattg ttgctgcctg attgactgga
    41221 ttggcatcga tgctgtaagt tgttgcatag ataatcatgc agccgatttg gatatccgaa
    41281 cgcaattcgt ggccgatttt tacggctttg ctacttgcaa cgaattgatt atgccatgct
    41341 tggaaaatat tttggtactc tcctgctcca ttgcttttta ctaatccttg acttaaagct
    41401 ggaaagtgaa aggccgaatt gatttcattg aaagtcatcc aatatttgac tttactgctg
    41461 tatctctcta atactgtctg agcaaatcgt tcatagaagt cgatcagttt tcgatttttc
    41521 cagccaccgt attcttttgc caaatgaaga ggcatttcgt aatgagaaat cgtgatcaca
    41581 ggttcaatat catattttaa gcactcatca atcagctgat cataaaattc taatcccgct
    41641 tcgttaggag taccttcgtc tccatttgga aaaattcgtg tccaggcaat cgaaaaacga
    41701 taacatttga atcccatttc cgcaaacaag gcaagatctt ctttgaaacg atcgtaatga
    41761 tcgattcctc gatgattcgg gtaaatatat ttttcttgat caatcgtcca gtcaaattct
    41821 tcactgccga taatcgcaaa gcgctgctta cctccaggca tagcatctgc aacggatagt
    41881 ccttttccgt ctcgatcata agccccttct aattgatttg gcagcagttg ctccacccca
    41941 taaaaatttt tctgaaaaac gtgattgcat cctgatttcc tccttatttt acaattgcca
    42001 gaacatcttc gtttgcaata atctcttttt ggtctaattc aagtatatcg gcaaatgcat
    42061 cagtattcgt aatgacaact ggagtgatta cgctatagcc agctgctcta attgcttcga
    42121 tgtcaaaacg aatcaataaa tctcctgctt tgacgagatc cccttgtttg actaagatct
    42181 caaagccatt cccatttaat tctactgtat ccatcccgat atgaatcaaa atctccgctc
    42241 cgtcagtcgt tgtgatccct acagcatgtc cagttggaaa aatagtcgtg atctctccgt
    42301 ctgcaggtgc aactagttct cctacttctg gatcaatagc gatacctttt cccatcacgc
    42361 cagaagaaaa aacttggtct ggtacttcac tcaatggcag tactttaccc gttaaaggac
    42421 tttttaaggt atgccgtgct gaagtaatcg gttttcctgc attcgcatgt ttgttttcta
    42481 attgattgtc ttgtgtttgt tcgtcaaatc ctaaaatcaa tgtacctacg aatgcaagaa
    42541 caaaagcaat tcctgtagct attactgcta ctgtcacatt tgactctaca ccatccaccg
    42601 tattaatgaa agtcggaatc gtcaataagc tgaccaaact gctggagaaa gcttttactc
    42661 cggaaaatcc gataatagct ccaccaatac ctccagaaat acaagcggca atgaatggtt
    42721 tcttcaaagg taatgtcaca ccatacacag ttggttcagt gattccaaat aacgaagtca
    42781 ctgtagaaga aatccctaat gctcgaagtt tcgtatcttt tgttcgcaat gctacagcta
    42841 aagcagcccc accttgtgcc aagatggctg gaagaagcat tggcatcaat acatcaaacc
    42901 catattgttc gatattcaaa aacatgatcg gtacgaatcc ccaatgcatg ccgaacatca
    42961 cgaatacttg ccataaactt cccatgatta gacctgctac gattggacta aatccataaa
    43021 tcgagttgaa gagaccgcct aaagcattac cggcaaccgt cccgattggt cctaaagcaa
    43081 gaaatgttaa tggaaccatc actactaaaa cgacaagcgg aactaaaatc atttgcagaa
    43141 attgcggaat cacttttttt acgaatggct ctaatttact ttgaacccat acggctagaa
    43201 tgattggtat gacagaagaa gtataaccag atggtgaaag aatcactggg atcccaaaga
    43261 aactgatagc ttctcccgca ccagcaactg ctgtgatagc tggatacaca agtgccatag
    43321 caattgctac tgctaaaaac tggttggctt tgaacttttt agctgctgta aaagcaagca
    43381 tgacaggtaa gaaggtaaag acaccatctg ctgcagcaaa caatatttga taagtaccgc
    43441 tatcagctgt cagccagccc atcaccgttg ccaatgataa gaatcctttc aataccccgg
    43501 cggctgccat cgcccctaga aaaggtgtaa atatactaga gataatatcg atcagacgat
    43561 tcaaaatatt tttggactct tcttttccat cctccgaaac tgcaagacct tcctcatcca
    43621 cgatcgattg ataaacatct gctacattac tgccaatcac tacttgatac tgaccgccac
    43681 tttgtacgac ttggatcacg tccgggtctt cttttaactt ttgcgtatct gctaaagatt
    43741 catcttttaa tctgaatcgc aaccgcgtag cacaatgtac taaacttttg acattctttt
    43801 gtcctcctac cgcttcccat acacgacgac caactgcttg attatccatt gttttttcct
    43861 tctttctttt ttatttaaaa aataaaaacc cagattgaaa aaaacagtca caaacattcg
    43921 tgaacctatt ttttcaatcc aggttttgcc tgccatacag taacaatcct tatgattgga
    43981 tgcttttttt ctcacttact actctttgaa tatgaatcgt taaataaatc tgctctgcga
    44041 tccccatttc ctgtttttgc gtttgtcgaa gatactcatt gatccgttcg acacaatgaa
    44101 acgcttttgg atactttcct tgaactaatg cgtagagaaa ttcatcttgc tcgtcagatc
    44161 cttccccttt gagataacgc tgggcaaaaa actgcagatg agtaatgata cgctgataat
    44221 tcaaagattc ttcatcgaac acgatgccga agaatttact gatgatactc agtatatctc
    44281 ttaccattgt ggtagcggtc atcgtcactt gcatgtcttc gtttccttgt tggctattca
    44341 caaaatgaaa ggcaataaaa ccggcttcat cctcagaaag ttccacaccc gtcttttgtt
    44401 tcacgaacaa aagagcctct ttcgctgctt gatattcttt cggataaaat ttttttgttt
    44461 caaataataa tggattaggc agttggatat tttcttttgc tcgtgtgatc gcataatgaa
    44521 gatgatcggt caatgtcaga taaatattag gcgaaaattc aacttttagt ttatcttcag
    44581 ccagttcgac tacttgtttg acccattcga tctcctctgc cggaatatca gccatcagct
    44641 gctggaattg gtttttagca aaagaatctc ttaaaacaaa ttctttttgg accgcttctg
    44701 gcgaaatctc gtccccaatc tttttttgaa aagctagtcc ttttcccata caaacgactt
    44761 cttccccgaa tccatttttc gaaatgacga cattgttatt caaaatttta tcaattttca
    44821 ttttgcttcg ccctctcaag aaaaataaaa aaacctagaa ttcgcctact gtttcttagc
    44881 tgaattctgg ttttgcctgc cgtacagtaa caatccattt cttattcaaa atcatcatac
    44941 catcgaattt ttatttatgc aagccttttc atataaaaaa taaaaaaact ggaacacaag
    45001 tctatctaga aaacaaccaa atcagagatg cacctctttg cgtttctctt tttttcagca
    45061 aattggtttt tccttatcga ttcatatcct ttggctgttt ttaatctgta acgacttatg
    45121 tcccagtatc ctaaaatgtt tttgtctcta gattgaaagc agatctcata tcatctgtcg
    45181 catcttttat cgatgtaaca ctttccagtt ctccttgtac aactagtcgc gtgacggcgt
    45241 ccatgtcgcc acaagtaatt agtgtgacaa tttcttttcc ctcgacttca ttcagtaatt
    45301 gggtatctgt aggagcaact ttttctttaa aaaatgtttt gtatgtatac acattttcta
    45361 aatctgtcaa atagatcttg gcacccatct ctaaattttc tagcggtgta aacagcaatt
    45421 cagggttcgt tgcacgatgg cttgctaagg cataatttcc ttttcccatt tcttgatctg
    45481 gagataatgt tcctgctccg taaagtagtg acgtattatc caagcctttg aaaaccggta
    45541 aattgattga aactgaaggg actgcgatac tagcaatcac tggtagatct gttcctctca
    45601 tttgcgacct catgacccct tctgtcgtca ttggttctac cgcatcaaag tcaaaactga
    45661 catctttatc gttattcttt ttcagatctt cttttgttac attcgctata gcatatttat
    45721 ctcctgtctc tctgacaaaa taatccttga tctgttcatt gaaaatcaaa gctagtccta
    45781 ctaataacaa gaggaacaag aggatattga ctagccatct ttttgctttt cctttttttt
    45841 tcattttctc tccctccaga ctcattttac tgtttttctt ttgctctttc caacagccga
    45901 atgacacatt tctacaactc tgtgtatttt tttcgtacac ctgcgctttt ttctacagga
    45961 tatttcaaat tatttttgat caatttttct tcaatgatct catctagatc caggtgaagg
    46021 ttgtctgcca tcatgtatga atagatcaat acatctgcca gttcttcttt gattcgttct
    46081 aattgtgttt cttttacttc ttccggcgtt ttccattgaa aaagttctaa caattcagat
    46141 gcttccaaag aaatagagat agccaaatct ttttcgttat gaaattgtcg ccaattacgt
    46201 tcatctctaa actggttgac tttttccata ctattcattt gcttgccttc ttccaatcac
    46261 ttcttacttg attaatcata ccattttttt acataaaaaa tagatgatgc ttttaaaaaa
    46321 actcctgttt tcttttattt aaaataaatg tcatagaaaa tcaataattt aggtagccac
    46381 catgtgtatg cagtagcgtg tcttcttccc ttgaggcttt ttgtttttcg atacgttctt
    46441 cttcccaaac agacaacgct actagaatct ctcttaatga ttttccttct tcggtgagcg
    46501 agtactctac tttaggcggg acttggttat aggcttttcg atggatactt tcatcttgtt
    46561 ctaactctct taactgctgg gtcagcattt tttttgtgat cgtagggatt tgcttgcttc
    46621 tccacgccta aaataagcga tattgctctt ccctttatct ggtgtaagca aagcagttag
    46681 cattttgacg atcgtgggtt ccgcttttca cttcttatct acttagtatc atgaagtata
    46741 ctttcgtact aatactaagt agcggaagtg tcaactactt tgtttaacta catagcaaaa
    46801 gaaacagaag ggtgacaacg gtcatcacgc ttctgtttct tttgaatatg ctgtgttgtt
    46861 gtctctatct ccgtgtagac tttcctcaaa cttatccttt gacagtaagt cagtaggctg
    46921 atttgctctt tctagatgcc agatacattt ctacaaaaaa atagtgattc tgttttgaaa
    46981 attcgatttt tagttctgca ttttcgtaaa catccaacat ttttttcgca ctgtatagac
    47041 ctaacccgcc atttttttta gaggtttttc ctcttttgac catctccgat agcgtcaaat
    47101 tcgttggatt tgccttgttc gctatactaa aacgtgatcc ctctttttct tccagcagat
    47161 tgatcgtaat cggactctga tcggctacac gatgatcgaa tgcgttagaa ataaaaatag
    47221 ataaacagcg aataaaaaca accagatcaa tcgggattgt tttcacttcg acaggaataa
    47281 ctacttggat cgggattttc tccgattctg ctttttctag aaattttaaa aagacacccc
    47341 tgacagctgg ggatacaata tttgttagct gttgtatttg cggtttttca aaaatagctg
    47401 cagaatgatc ttctaatatt tctaaaaatt ccaacgtttc ttttttattt tcctgaagag
    47461 taatttttaa tgaaaacagt agacttttat aatcatgttt gaattcctct agatgatggt
    47521 aatattcttc atttatttga tacagttgaa gcgattcctg atgttgtctt tcttttactt
    47581 ggaaactgat caacataatg gaaaaaaaca aagaaaaaaa actgatgact atcgacaaaa
    47641 gagatagaag cagataatta gatgagcgga agtgaacact tcccaatacc aagtaaatca
    47701 cgccccagct taataaaata caaatacctc caaaatagtg ttcagttgga taagtaaaaa
    47761 gcaagttgag aaatctcgtc tgtttgtcta tactttttac taatagatag aaaataaatg
    47821 cgataagtag tgcaaaaaaa cttgctgttc caagatgtct ctttcctaaa taatccatca
    47881 gatcaaaaac cagaaaaaag ctgagtccag tgatagcaaa aaaaagatta ctgatcaaac
    47941 agctgataaa caggtttttc gtagtaaaat aaaaaagaaa catcaataat aagccaacta
    48001 ggtaaggaaa gctcacttta gggataggaa gtaaaagtgt caccaccatc aaagctatac
    48061 aaccaaagct gttttttact agcagttgtt tcgaaaagaa taaatagata cctaaatcta
    48121 aaatcaaagt aaaggtcaaa taaagtgtca ctgttgcgac catcttattc ctccattgtt
    48181 ttgtttcaaa atgtacacat atttttttaa taggagcctg catatgaaaa agtattttat
    48241 catagaagat aaccagtttc acagcgaatg gatcatcaga gaattggtca atcatcatat
    48301 cgagaatatc gtgcagctca cttctatctc tcaaatcaat cagttgatcc agttgggcac
    48361 tattgataat cacgatgttt tctttatcga tatcgacttg aagcaatcac tcacaggtat
    48421 tgatgtcgcc gaacaaatac gtaaaaaaaa tgcacatagt aatattgttt ttttcaccgc
    48481 ttatacgaat tttgcagtag agatcatcaa tcggaaaatc cttccgattg gatatatcag
    48541 aaaagacagg aatttatctg aacaaattcg tcaagtgcta gaacagatca attggaaaga
    48601 acagcagatc gtaaatgtcg ataaaatctt tattgatcaa aaaggtggaa gtctcgtcct
    48661 gtatcctcgg gaaattttat atattgaaag tatcaaaggt ttgaaaaacc aagttatggt
    48721 caaaacaact acagaagata aattagtgaa tacttcttta aaacaattga agacagagtt
    48781 agaaaagtat gcttactttt tacttttaaa atcttactgt atcaacttat cgcaattgca
    48841 gaaaattgat tctgctcatt atgtgttaga atttttcaac ggtgactcct tactcgttgg
    48901 tcgaaagatc tttgaaaaga caaaggaacg tttccatgac ttccaaaaaa ctgcttcgag
    48961 ttgatgaata agagctgaga cgtaagcata gcagatttca gggagtgggc cttgaatcgg
    49021 ctccactccc tgaaatcgtt gatatgattt tacgcttcct cttctgtagg agcatctgtt
    49081 aacgaccatg tgaatgttga aacgtagttg tctacagcat aattggatac aggaacaaat
    49141 aattccactg attgtctccc ttgttccaca ttctctccta atgaataaat ccatgtgcca
    49201 atcccttcat ttgctcctgc agttactaga tctactgcct gatctgctgg gatttcgatt
    49261 ccttcagcgc cagttccaga aacggtagat ggcattccac tgctattagt tgcgctattt
    49321 cgaatcacgg cgtttttgaa tttcaccttt gaaccagtta gtttattcgt atcagatgtt
    49381 gtaccaataa attctgttcg tttgactgat aatttccaac cggcataatt tccgctttta
    49441 tccgttactt gtacgaaatt ctgtacttgt gtttgtgctc cagtactgtc cgttccgctt
    49501 tgtaatgcag catatactgt ttttgctgta cctaacttga ttgttccaaa atcaagatct
    49561 ggagcttggt caatcgtcag taatccggat gttccctgat ttggttcgtc aggtgtaaaa
    49621 ggagtatcag gatcatttgg atcaacagga ggaactggaa caacgtcttc aggatttgca
    49681 gcatctggtt tggtcacaga aatcgttcct tctgtagtgg ctgagctctt attctcagca
    49741 aaaacacttt gtgtacataa taatagactt ccagctagaa cggtacttaa tgcaattgtt
    49801 tttttcattt ctttttcctt ctttctttta aaataaataa tccagcaaat aagccaataa
    49861 gaatgactcc aacaagtatc agccatcgcc agtcaaatgt ttctggctga ttttttaaat
    49921 caatatcctt ttcattcaat tggtccgctg catcatcagt tatttcaaaa tcggtatgga
    49981 gattccagcg tttttctttc caagcaccct tcacaaccgc ttgataatgt cccggagcta
    50041 atttttttcc ttctaatggg acaagtaaat caaaatttgt attgggtgct acatctattt
    50101 tttctttttt tattgaaagg taaggctctt ttgtttcttt tttgtagatt tcaaccgatg
    50161 tagtcaagcc gaacatataa gaagcatttg gattttgaag attcgcctta atcacatttc
    50221 ttccatcgct ttgatccgca taagcttttt ttaatttcaa atctggggaa gactcttttt
    50281 cgctatttca aatcaagatt gctttcactt gagaaaactg attgacgaca ccaactgtac
    50341 ttttgttaga aacgttctct tttttttctt gaaacgtgat ccctcctgcg accacaccag
    50401 ccagctcttt ttctggcgct ttcagtgaaa gtgtctcagt gaccgcactt tgaggaggaa
    50461 cagttatttc tcccactgtt tttgctaagt ttttcatatc ataacttaat gtttcatcaa
    50521 atttactttt tgattctcca tattcgatca caccgtttga attcgtcgta gcggagtgga
    50581 tcaatgtctc aactgttact gatttatctg ttcggttaaa gaccgtgtag gggatttcct
    50641 gtgttttccc aggctccatc cttacattta tatatgtttt gttcttatca atctgattag
    50701 ccggtaattc aaaatctacc gcaaaattga attcggaggc atggacaggt gtctgccaaa
    50761 ggatgaatcc aaggagaagg atcataagta aagagcagtt gtttcgatga cttttattcc
    50821 atattcgatt catcaatcat cctccttgat ctttattctg tcggcgcatc tgctaatgtc
    50881 catgttatct ctgctgagta attaccagct atctgatttt ctttggttaa gacaagttta
    50941 aacgcccgac cgttatttaa atattctgaa taatctgctt gcacattcgt tgaattttgt
    51001 cctctgacaa tttccagttc ttcttcattc actaattgat actcctctcc tttcttgaaa
    51061 tataggacat cctgcataga aaatcctgca tcatttgtga aaggctgaga taattttatc
    51121 gttaagatcc acggattttt cgtttctcgc tgatcataaa tccataattt ttcctgtata
    51181 tccatttggc caaatgcttc ttgtgtcaaa ccagttgctt tgatctgacc aaaatcgagt
    51241 ttggaagggg caagaaatga gagaccacct tctactacga tcttcgtagc aggtgcaaaa
    51301 ggcggggcat catgataaac aactggcgta tcacttggat tgtgattccc aatttcatcg
    51361 ttggtacttg gcttgttctg cacaccctta gcagtagctt ctcctgcttg gtcgtttaat
    51421 acaatttcta atacatctcc tgcaaaaagt tgattttctg ggatagaaag aacaaattcc
    51481 cctgtatcat caatagttgt ttctgacaaa acagcttgat tttttttcaa tgaaaccaag
    51541 gctcctgctt cacctgtacc tgtgatcgtg cgagaagctg cattgatcaa tgtggtactt
    51601 aaattagcag gttcaggagg aatcacatcg actgtcgttt tggcagagtc cagttctgtc
    51661 acttcttggc cacctcttac ttttttagca gcaacaactt gcacttgatc ctccgttttt
    51721 aatctctcat cattgggtac ttgaacttcg aacaagccgc cttgttcgcc ttctccccat
    51781 gggttatatc ctgccccttc attttcatct gtttgtcctt gcgtgagagc agtaatttct
    51841 acagatgatt tcgtcgtatc tgcgtaagta atacgtaaag tcacttctac ttcgcctgtc
    51901 caagcatctc ttggctttgc ctcgacacct tcgggaacaa cgacatgccc atagattttt
    51961 ttatctgcat tcgttggcac tcttaaggat tcaatcatcg ggagctgatt attggcactg
    52021 attcgattat aatttttcat tccggcacct gtctgagcaa attcatttgt taaacctgca
    52081 ctagggcttg atacgaaatt gcctaagtcg gtcccattga tcgataagtc tgtcatatca
    52141 gaaaaataat ccgggtcttt ttcaaagtta tcgaatgttg cgcctgtttt tcgccataaa
    52201 gcgatttctg aatgggcgat ctttaaagaa gaatctccat ttccttggaa tacccagccg
    52261 cctatgccat tggtctcgct tccaatccgt ttgttttgga aatcaaaata ggtaggagat
    52321 tctaagtcca cattcaacac atccccactg atgatgccgg tcttatcttc ccctgttttc
    52381 ccgaccatga ctaaataagt tttttcacct gccgtaaaat ttaaatcaat atctccagtt
    52441 gtatctacag ttgctccgga atctgcgcgt atatcgatat ttgatttttc tccagtgact
    52501 gtaaaatcag ctgaacctgg actttctaga aaaccagaag cgtcataaaa ttggatcgct
    52561 tgatttcctt gattgagtct gttcacgtaa gatttgccat cacctgtatt tcttaccctc
    52621 aatgagcctc cattggttac tgtcagcttt tgcgtaccat tctcaaaacg aactgcgctg
    52681 gaacgcccac tctgcttatc gatgtcgata actgatccgg ctccttctac gatcaaagaa
    52741 aggccttttc ctacaaaacg catcccattg ttcccgggtt gatcgtcatc tccagccatt
    52801 ttgaccgaga tttgtccacc acttgataca tggatttgac tagaatctcc ttgcaagcga
    52861 aatccgcccg tagttgctgt attgatggtg attttcccac cattcgatac agtgactttc
    52921 gatcctgaac ctctcatttg gatgattccg cgattgttgt cattggtaat attgatcttt
    52981 gtatttaccg aaaattcgct tcccgaacca ctgacttcaa acatgacatt accaggtagc
    53041 gtatcgactg cctctccttc atcgctaacg ccatctgccc gatcataatc aagagagacc
    53101 tttgaaccgc ttaagatatg gattcctggc gctgtttttc cactaccagt ctcatctcct
    53161 gaatctagaa aaccgtagaa tttccctaga tacggtccat aaagttgact accattgatc
    53221 acattcaatt gatagcttct acaaaaatag gctttacccg gagcggcatt cagtccttcc
    53281 ttcactggtt ggacatccaa cacaccggaa acaccatcta atgtgacagt tgcacgagga
    53341 gcgtaaatca gtcctaattt ggacccatca tacaaatcaa gtgtcccggt aaattttatt
    53401 tctcccgtaa aatgatatct gcttccccaa aaatcggtca tataagcaat gataatggca
    53461 tttccatcga tatttggtgt caatccacct ggatgtgcag ctgttccttt aaaattctgg
    53521 aactcgatcg actgaggtat ctcattgata taaatatggt gtgtgccgaa attgtgagcg
    53581 aatccaccaa aatcaataac gatatctcgt ttgatctcta attccctgtc taaagtcaaa
    53641 ggctgaatca gtcggatctt agttactgat tcgtctgcga gaagctgcgc cagttcttct
    53701 gttgtggcag cttcgctgta cccatccgct gaaacagctc ttgccagtgc cgcttcactg
    53761 ctatcagacg tttgttcagc tacttctgag gtctcacttg tcttattttc ttcttgcctt
    53821 ttattagtag aagcctcact ttgagattct gtagtttcag aagtaatcga ttcttctgaa
    53881 aaagtagtct tgtcaaaact ttgattctct gattgcgttg attcgtttgt tttctctaca
    53941 tcggcaaaaa cggatgtcac catcaagaga gggaagacaa acaaaatcga cgcaatcagc
    54001 ttttttacac attgattcat tctc
//
LOCUS       DANKAZ010000007        53325 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000007 DANKAZ010000000
VERSION     DANKAZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(61..540)
                     /locus_tag="PWY67_000381"
     CDS             complement(61..540)
                     /locus_tag="PWY67_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL2056692.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            899..1681
                     /gene="rpsB"
                     /locus_tag="PWY67_000382"
     CDS             899..1681
                     /gene="rpsB"
                     /locus_tag="PWY67_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL2056693.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1780..2661
                     /locus_tag="PWY67_000383"
     CDS             1780..2661
                     /locus_tag="PWY67_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL2056694.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2797..3519
                     /locus_tag="PWY67_000384"
     CDS             2797..3519
                     /locus_tag="PWY67_000384"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL2056695.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            3522..4079
                     /gene="frr"
                     /locus_tag="PWY67_000385"
     CDS             3522..4079
                     /gene="frr"
                     /locus_tag="PWY67_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL2056696.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            4274..5086
                     /locus_tag="PWY67_000386"
     CDS             4274..5086
                     /locus_tag="PWY67_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL2056697.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            5083..5883
                     /locus_tag="PWY67_000387"
     CDS             5083..5883
                     /locus_tag="PWY67_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL2056698.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            6044..7312
                     /gene="rseP"
                     /locus_tag="PWY67_000388"
     CDS             6044..7312
                     /gene="rseP"
                     /locus_tag="PWY67_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL2056699.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            7380..9089
                     /locus_tag="PWY67_000389"
     CDS             7380..9089
                     /locus_tag="PWY67_000389"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL2056700.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            9301..13659
                     /locus_tag="PWY67_000390"
     CDS             9301..13659
                     /locus_tag="PWY67_000390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL2056701.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            13804..14277
                     /gene="rimP"
                     /locus_tag="PWY67_000391"
     CDS             13804..14277
                     /gene="rimP"
                     /locus_tag="PWY67_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL2056702.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            14300..15475
                     /gene="nusA"
                     /locus_tag="PWY67_000392"
     CDS             14300..15475
                     /gene="nusA"
                     /locus_tag="PWY67_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL2056703.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            15497..15790
                     /locus_tag="PWY67_000393"
     CDS             15497..15790
                     /locus_tag="PWY67_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL2056704.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            15787..16098
                     /locus_tag="PWY67_000394"
     CDS             15787..16098
                     /locus_tag="PWY67_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL2056705.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            16111..18417
                     /gene="infB"
                     /locus_tag="PWY67_000395"
     CDS             16111..18417
                     /gene="infB"
                     /locus_tag="PWY67_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL2056706.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            18444..18791
                     /gene="rbfA"
                     /locus_tag="PWY67_000396"
     CDS             18444..18791
                     /gene="rbfA"
                     /locus_tag="PWY67_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL2056707.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(18948..19121)
                     /locus_tag="PWY67_000397"
     CDS             complement(18948..19121)
                     /locus_tag="PWY67_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2056708.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            19569..21931
                     /locus_tag="PWY67_000398"
                     /pseudo
     CDS             19569..21931
                     /locus_tag="PWY67_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            22299..23222
                     /gene="truB"
                     /locus_tag="PWY67_000399"
     CDS             22299..23222
                     /gene="truB"
                     /locus_tag="PWY67_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL2056709.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            23226..24167
                     /gene="ribF"
                     /locus_tag="PWY67_000400"
     CDS             23226..24167
                     /gene="ribF"
                     /locus_tag="PWY67_000400"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL2056710.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            24519..25304
                     /locus_tag="PWY67_000401"
     CDS             24519..25304
                     /locus_tag="PWY67_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2056711.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            25317..26168
                     /locus_tag="PWY67_000402"
     CDS             25317..26168
                     /locus_tag="PWY67_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL2056712.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(26292..26744)
                     /locus_tag="PWY67_000403"
     CDS             complement(26292..26744)
                     /locus_tag="PWY67_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2056713.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27209..27577)
                     /locus_tag="PWY67_000404"
     CDS             complement(27209..27577)
                     /locus_tag="PWY67_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL2056714.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            27727..28209
                     /locus_tag="PWY67_000405"
     CDS             27727..28209
                     /locus_tag="PWY67_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL2056715.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            28246..29256
                     /locus_tag="PWY67_000406"
     CDS             28246..29256
                     /locus_tag="PWY67_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL2056716.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(29371..30084)
                     /locus_tag="PWY67_000407"
     CDS             complement(29371..30084)
                     /locus_tag="PWY67_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056717.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(30274..30864)
                     /locus_tag="PWY67_000408"
     CDS             complement(30274..30864)
                     /locus_tag="PWY67_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL2056718.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            31348..31575
                     /locus_tag="PWY67_000409"
     CDS             31348..31575
                     /locus_tag="PWY67_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL2056719.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            31591..32757
                     /locus_tag="PWY67_000410"
     CDS             31591..32757
                     /locus_tag="PWY67_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL2056720.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            32991..34034
                     /gene="hrcA"
                     /locus_tag="PWY67_000411"
     CDS             32991..34034
                     /gene="hrcA"
                     /locus_tag="PWY67_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL2056721.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            34128..34691
                     /gene="grpE"
                     /locus_tag="PWY67_000412"
     CDS             34128..34691
                     /gene="grpE"
                     /locus_tag="PWY67_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL2056722.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            34742..36571
                     /gene="dnaK"
                     /locus_tag="PWY67_000413"
     CDS             34742..36571
                     /gene="dnaK"
                     /locus_tag="PWY67_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL2056723.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            36722..37888
                     /gene="dnaJ"
                     /locus_tag="PWY67_000414"
     CDS             36722..37888
                     /gene="dnaJ"
                     /locus_tag="PWY67_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL2056724.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(37970..38635)
                     /locus_tag="PWY67_000415"
     CDS             complement(37970..38635)
                     /locus_tag="PWY67_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2056725.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            38611..38784
                     /locus_tag="PWY67_000416"
     CDS             38611..38784
                     /locus_tag="PWY67_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056726.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(38829..39566)
                     /locus_tag="PWY67_000417"
     CDS             complement(38829..39566)
                     /locus_tag="PWY67_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2056727.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(39568..41070)
                     /locus_tag="PWY67_000418"
     CDS             complement(39568..41070)
                     /locus_tag="PWY67_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL2056728.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            41329..43323
                     /gene="uvrB"
                     /locus_tag="PWY67_000419"
     CDS             41329..43323
                     /gene="uvrB"
                     /locus_tag="PWY67_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL2056729.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            43333..46149
                     /gene="uvrA"
                     /locus_tag="PWY67_000420"
     CDS             43333..46149
                     /gene="uvrA"
                     /locus_tag="PWY67_000420"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL2056730.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            46412..47677
                     /locus_tag="PWY67_000421"
     CDS             46412..47677
                     /locus_tag="PWY67_000421"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL2056731.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            47681..48412
                     /locus_tag="PWY67_000422"
     CDS             47681..48412
                     /locus_tag="PWY67_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL2056732.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            48547..49431
                     /gene="rapZ"
                     /locus_tag="PWY67_000423"
     CDS             48547..49431
                     /gene="rapZ"
                     /locus_tag="PWY67_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL2056733.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            49428..50426
                     /locus_tag="PWY67_000424"
     CDS             49428..50426
                     /locus_tag="PWY67_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL2056734.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            50460..51395
                     /gene="whiA"
                     /locus_tag="PWY67_000425"
     CDS             50460..51395
                     /gene="whiA"
                     /locus_tag="PWY67_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL2056735.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            51938..52588
                     /locus_tag="PWY67_000426"
     CDS             51938..52588
                     /locus_tag="PWY67_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056736.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            52775..>53325
                     /locus_tag="PWY67_000427"
     CDS             52775..>53325
                     /locus_tag="PWY67_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL2056737.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGV"
BASE COUNT        17945 a         9154 c        11465 g        14761 t
ORIGIN      
        1 aataaggaaa atattgaaaa taaaaaaaca cgcattccat attaggaata cgtgctctga
       61 ttaaaaaagt gatacttcag ttggatggtg aagaaattct tctactcgtt ttgcgtcctc
      121 ctcattttcc gagatgacca tgatcgtatc gaatccagca attgtactga caatagaggg
      181 aggtttgatc tcatctaaaa cagatgcaaa taaatgagca ttgtctggca aggtattgac
      241 gatcgtcaaa aagtggactc gttcgacttt caatacaatg tcttcaatca tttgaaccag
      301 ccgatcctct tcctcagtcc tagtctcagg agttcctttt tcgcgaaaca atacgaagcg
      361 ggaaacgcca ttttcatcag gtgccttcac gatttttaga tcacggatat ctcgagaaat
      421 cgttgcctgc gtggcggtaa cgccataatg cccaagtttg tttaatagtt cttcttgcgt
      481 acggatcggg aattcttcaa tgacttgttt aatcagcaaa tgtctttgag ctttgcgcat
      541 tttctttctc ctttacgaaa gatttcacat gtatcataac aaaaaaaaga aggattgccc
      601 aataatattc gtcttttata cctaaaatta ttttatggaa aaaggataat aaagaaacaa
      661 agagaaatct actgtttttc ttgaattctt tttatctttc aggtaaacta ttgaatgttg
      721 agcagaaaag tatatttgct cttgcgatac ggatcttttg tctgtataat agctgagggt
      781 gctttatgca tcagcaaatc cacattctta ttggattgtc ggtaggtgct tgaaaaagtt
      841 tgccggcaag gtagaagata agaagaggaa aaataaaacc aaatcggagg aaacaaacat
      901 ggcagtaatt tctatgaaac aattactaga agccggcgta cactttggtc accaaactcg
      961 tcgctggaat ccaaaaatga agaaatatat cttcacagaa agaaacggaa tctacatcat
     1021 tgacttgcaa aaaacagtta aattagtaga tgcagcttat gattacatga agagcgttgc
     1081 tgaagaaggc ggcgtagcat tattcgtagg tacaaaaaaa caagcacaag aagcaatcaa
     1141 agaagaagca actcgtgcag gacaatactt cgttaaccat cgttggttag gcggaacatt
     1201 gacaaactgg gatactatcc aaaaacgtat cagccgtttg aaacaaatca atgcaatgga
     1261 agaagatgga acttttgaag ttctacctaa aaaagaagtt gctggtttga acaaagaacg
     1321 tgaacgtctt gaaaaattct taggcggtat cgctgatatg ccaagaatcc cagatgtaat
     1381 gtacatcgtt gacccacgta aagaacgtat tgctgttcaa gaagcacaca aattaaatat
     1441 cccaatcgta gctatggttg atacaaactg cgatccagat gaaatcgacg tagtaatccc
     1501 ttcaaacgac gatgcaattc gtgccgttaa attgattact tcaaaaatgg ctgatgcttt
     1561 catcgaagga aaccaaggtg aagatcaagt agttgaagaa gatttcacag aagaaaacac
     1621 agcaacatca atcgaagaaa tcgtagatgt tgttgaaggc gacaattctt cagcagaata
     1681 attttgaatg atggagctgt ttcaaaggct gggcgacaag cctcactctc ctttgaagca
     1741 gcttttttta aaaagaaaaa cacacaggag gagttaaaca tggcagacgt tacagctaaa
     1801 atggtaaaag aactacgcga aatgactggc gtaggaatga tggatgcgaa aaaagcatta
     1861 gttgaagtag aaggcgacat ggaaaaagca gttgatcttt tacgtgaaaa aggaatggca
     1921 aaggctgcga agaaaaatga ccgcattgca gctgaaggat tggcttcagt tgctgtgaaa
     1981 ggtaacactg cagcaatcgt tgaagtaaac tcagaaacag actttgtttc taaaaacgaa
     2041 atgttccaag atttagtaaa agaaattgca gaacttgttg ctgaaaacaa accagcagac
     2101 atggaagctg caatgaaaat caaaacagat aaaggtacaa ttgaatctga cttgatcgaa
     2161 gcaactcaag ttatcggaga aaaaatcagc ttccgtcgct ttgaagttgt agaaaaagac
     2221 gacaatgctg catttggtgg atacttgcat atgggcggac gtattgccgt attgactgtt
     2281 ctagatggaa caactgacga aacagttgca agagacgtag ctatgcacgt agcagctatc
     2341 aatcctcgtt atgtaaacga atcacaaatt ccagaagctg aattagaaca tgaaaaaact
     2401 gttcttacag aacaagcatt gaacgaaggc aaaccagcga atatcgttga aaaaatggta
     2461 gaaggacgct tgaagaaatt caaagcagaa atcgctttgg tggaccaacc attcgttaaa
     2521 gatcctgata tgactgttga aaaatacgtt gcatcaaaag gcgcaactgt taaaacattc
     2581 gtacgtttcg aagttggcga aggtatcgaa aaacgcgaag ataactttgt agaagaagtt
     2641 atgagccaag tgaaaaaata attctgttga ataacagacg ggaacgcacg gtttgtgcgc
     2701 tccctttttt taagcaaaac aagaaaaacc agtcttagag tgtcgaaatc atctaagtat
     2761 ggtaaaatgg caatagaaaa tattggagga atgaacatgg tcacaccgaa atatcagcgt
     2821 gtagtcttaa aattgagtgg agaagcttta gctggggacg caggatttgg aatcaaacca
     2881 ccagtaatca aagaaatcat ccaagaaatc aaagaagttc acgagctggg cgttgaaatg
     2941 gcaatcgttg tgggcggcgg aaatatctgg cgcggacaaa tcggagcaca aatgggcatg
     3001 gaacgtgctc aagctgatta catgggtatg ttagctacag ttatgaatgc cttagcttta
     3061 caggatacgt tggaaaacgt gggtgtaccg acacgcgtgc aaacttcgat tgaaatgcgt
     3121 caaatcgcag agccatatat tcgccgtaaa gcagaacgtc atttagaaaa aggacggatc
     3181 gttatctttg caggcggtac cggcaacccg tacttctcga cagacacaac agcagcatta
     3241 cgcgcggcag aaatcggcgc agatgttatc ctgatggcta aaaacaatgt agacggtgtt
     3301 tattctgctg atccgaaagt agatgcaaat gcagtaaaat ttgaagaact gactcatctg
     3361 gaagttattg ccaaaggatt acaagtcatg gattcaactg caagttcact aagcatggat
     3421 aatgatattc cactgcttgt cttcaattta aatgaacacg gaaatattcg ccgtgcaatc
     3481 ttaggcgaaa atatcggaac aactgtaagg gggaaataaa aatggcagat gcaattatga
     3541 cagaagcaaa agaaaaaatg aaaaaagcag cacaaaatct tcaacgtgaa cttgggcaaa
     3601 tccgcgcagg ccgtgcgaac gctagccttt tggaccgtat tactgtgaac tactacggtg
     3661 cgccaacacc attgaaccaa atggcatcaa tccaaattcc agaagcacgt gttttaatga
     3721 tcacaccttt tgacaagagc attcttcaag atgttgaaaa agctattatg gcaagtgata
     3781 tcggaatcag tccaaccaat gatggaaatg tcattcgctt agttatcccg caactgacag
     3841 aagaacgcag aaaagaatta gctaaagatg ttaaaaaaga agcagaaaat gcaaaaatcg
     3901 cgattcgtaa catccgtcgt gatgcgatcg atgaatataa aaaacaacaa aaaaatggtg
     3961 atattaccga agatgatttg cgcggattag aaaaagacgt acaaacattg acagataaca
     4021 gcatcaaaga agtagataag atcgctgctg ataaagaaaa agaattattg gaagtttaat
     4081 aaaatcataa aaacagaggg gcggatcatt ccgcttcctc tgtttttatt tatatttctt
     4141 ttatttaaaa attaaaaaaa gaaaaaatta ttcagttttt aaataaaaaa acaatgattt
     4201 cataggaaaa acagttagat attgctataa tacttaagat agtgggaatc ttttaagtat
     4261 aggaggaaaa agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag
     4321 cgaatttcaa tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg
     4381 caatggtcgt tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat
     4441 ggaaacagta aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct
     4501 ttatgccttt tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca
     4561 actacctgtc gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt
     4621 tcatgtcatg ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc
     4681 aattgagcaa acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag
     4741 cagagcagag atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat
     4801 acacgcagaa gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc
     4861 taaagagtta caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa
     4921 ttttctgtta tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga
     4981 ttttgatggt gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt
     5041 cggcggtgta aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtggtc
     5101 acagcagccg tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt
     5161 gagcttacag cagcaatctt agcagtaata ggagtttacg agctgtttcg gatgaaagga
     5221 ttgacactct taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg
     5281 ccaaaagaaa gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat
     5341 cttatagtta tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat
     5401 gaagcaggtt ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc
     5461 aatgcacgaa cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact
     5521 gatatcggag catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt
     5581 tcgcccaaca aaacgattga aggtgcgctc ggtggtattg gtagtgctgt tgtagtcgca
     5641 tttctttatt tattgctata cccagcaaaa gaactattta atcatggtat gtttgtcatg
     5701 cttctgatga cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca
     5761 atcaaacgtc attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc
     5821 ttagaccgtt tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt
     5881 tagtaagtat acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca
     5941 ttatattgat actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt
     6001 gttttataaa aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac
     6061 atttatcatc gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc
     6121 gaaacgatca ggaatcctcg ttcgcgaatt tgctatcggt atgggtccga aaatttatgg
     6181 gcatcaagca aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt
     6241 tcgaatggca ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct
     6301 tttactaaat tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac
     6361 aaatgctatc ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg
     6421 ttatgtgaat ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat
     6481 tattgaaaac gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc
     6541 taaattgtgg cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc
     6601 aatcctgtta ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa
     6661 tcgcgtagga gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga
     6721 tgaggtagtc agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat
     6781 tacgaaaaat ccggataaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc
     6841 tgtcgacgtg acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat
     6901 aaaagcccca atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt
     6961 cagcggttca ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga
     7021 taaattaggc ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt
     7081 aacaactgtt attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct
     7141 tccgattcct gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg
     7201 gaaaccatta agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat
     7261 gttgttgatg gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt
     7321 gacaaatagg gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa
     7381 tgagacaatc aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat
     7441 taagccatca aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt
     7501 catatctgcc attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat
     7561 ttgaaaaaat cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg
     7621 aagaatccgg gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg
     7681 atcgaaaaat gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg
     7741 aaatcaattc atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg
     7801 acgaaaaacg acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcgt
     7861 attctttcca tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct
     7921 acaccgaagt attcaagcgt tgtggcttag aatttcgctc gattatcgga gacggtggcg
     7981 caatgggcgg gaaagactca aaagaattca tggcgatctc agaaatcggg gaggatacga
     8041 tctgttattc gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg
     8101 tatcaaaaaa atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa
     8161 aatcaattga cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg
     8221 tattgttcat tgcggatgaa caacctgtcc ttgttttggt acgcggagac catgaagtca
     8281 atgacgtaaa actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag
     8341 aagcacaaaa atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg
     8401 acgtaaaagt atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga
     8461 acgaaacagg attccactat aaaaatgtga atccagaacg cgatttcact gtactttctt
     8521 atgaagatct tcgttttgta caagaaggag atccttcacc agacaacaat ggtgttcttg
     8581 cctttactcg aggaatcgaa atcggacata tcttcaaaat cggcactcgt tacagtgaat
     8641 ctatgggagc aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt
     8701 atggtatcgg tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag
     8761 gaatcaactg gccaaaagga attgcgccat ttgatttgca tatcgtccaa atgaatctaa
     8821 aagacgaata ccaaacaaac ctgacgaatg aactagaaga agcgatgaca aaagctggct
     8881 atgaagtact ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt
     8941 tgattggctg tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag
     9001 tgaaaatcaa gaaaactggc gaaatggttg aagtacgtaa agaagaattg gtgaatactt
     9061 taccaatcct cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc
     9121 tgaatcagct gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa
     9181 tcgcagttcc aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga
     9241 aaaagaagag gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct
     9301 ttgtcagaaa aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca
     9361 gaaaaaaatc atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa
     9421 tcgcgtttat gggaatttac gctgcatttt tccaaaattt tgcctattat gctatatcgg
     9481 tcattgactc agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc
     9541 ttagcagatg accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg
     9601 gaaaatcagc aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg
     9661 ataaaagatc gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag
     9721 caacaatact tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt
     9781 atcgaaccag aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc
     9841 aaacaagaac aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa
     9901 caaaagaaaa aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag
     9961 ctaggacgaa atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa
    10021 gaacgaagag tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca
    10081 aaacgcaaga tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa
    10141 ttctcgaacg gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta
    10201 aaggtacgag gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa
    10261 gatatcatag aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga
    10321 gtagagcttc atgttcatag caatatgagt acgatggatg caacaaacag catctctgat
    10381 cttgtggcac aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt
    10441 gcgcaagcct ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac
    10501 ggcgtggaag cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa
    10561 gcattgagtg aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta
    10621 tatgacacga tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc
    10681 tttgatgaat ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt
    10741 atcacggatg gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt
    10801 gaattttcaa aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg
    10861 aatactagct acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg
    10921 gaattagcta gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa
    10981 aaatttggtg tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt
    11041 cacttagcat ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat
    11101 cagctaaatg agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta
    11161 acgattttag caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg
    11221 aatgtcgaat acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt
    11281 gaaaatctat tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa
    11341 aaaggggtag aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca
    11401 aaagcggtct atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa
    11461 gaaattatcc aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca
    11521 ggtaatgtac attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct
    11581 atgggcggag cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc
    11641 gatgagatgc taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta
    11701 gaaaatacga ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt
    11761 tatacaccga agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca
    11821 aaacaaatgt acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg
    11881 aattctatta atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat
    11941 aaaagtaatg aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt
    12001 gcgacgatga caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag
    12061 tgccaatatt ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa
    12121 aaacaatgtc caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag
    12181 acatttttag gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat
    12241 tatcaagcag aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt
    12301 gccggaacga ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa
    12361 agggacaata atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact
    12421 ggagtcaaac ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg
    12481 gatgtctatg attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa
    12541 acgactcatt ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg
    12601 catgatgacc caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg
    12661 atccctacgg atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta
    12721 agtcaggaac aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt
    12781 tttgttcgag ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt
    12841 tccggactat ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga
    12901 ggagatgcga cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc
    12961 catgctggat tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa
    13021 tggaacaaga tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat
    13081 gtgcccgact ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat
    13141 gctgcagctt acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt
    13201 ttgtattact gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc
    13261 aaaggaaaag atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct
    13321 tccgtaaaag aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga
    13381 ggattcaaat ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa
    13441 ggtgatacgt tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa
    13501 caaatcgttg aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc
    13561 ggtaaagttt caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta
    13621 ccagatgaaa atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct
    13681 tgtaatttta agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag
    13741 cggatttttc gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa
    13801 cttttgagta gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa
    13861 aattttgaac ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta
    13921 tttattgaca aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg
    13981 agtgaaaaac ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta
    14041 tcctccccgg gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt
    14101 gattatgtcc atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt
    14161 cttcaatcat ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa
    14221 gaattgactt ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca
    14281 cggagaggaa tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc
    14341 tgaaaaaggg atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc
    14401 atacaaacgt cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg
    14461 aaagatccat gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga
    14521 agtctcattg aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg
    14581 ttttgaagta acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat
    14641 tttacagcgt gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga
    14701 aaaagacatc atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct
    14761 tggtaaaatt gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc
    14821 acatgatcga atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt
    14881 atttgttagc cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga
    14941 agtctatgat ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa
    15001 agtggcagtt cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa
    15061 gggacaacga gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga
    15121 atggaatgaa gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga
    15181 tgtgatcttc gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt
    15241 atcgctagca atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca
    15301 taaaatcgat atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca
    15361 aaaagaaaca gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga
    15421 atatgaaaca attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta
    15481 gtaaggaggc aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg
    15541 tgaaatgaaa cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc
    15601 gatcgatcca actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga
    15661 agtacaacaa gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga
    15721 tgagttttat caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt
    15781 tggaaaatga atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc
    15841 gcgggaaaac taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca
    15901 aaattggtgt ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag
    15961 agttcatatt atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg
    16021 atcggaaaac ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag
    16081 gagctaattg aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca
    16141 aaagaactca aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat
    16201 gtgaaaaacc atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc
    16261 aaaaacccaa gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa
    16321 cagccgaacg atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac
    16381 cgcagttatc aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca
    16441 acaaaccaaa ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc
    16501 ggcaatcaaa accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt
    16561 aataataata accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca
    16621 actaaaccag ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact
    16681 gaaggtatga acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt
    16741 aaaaaattat ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg
    16801 attgaactgt tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt
    16861 gctgatatcg ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt
    16921 ccgccagtcg taacgattat gggacacgta gaccatggga aaacaacttt attggataca
    16981 ttacgccatt cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc
    17041 taccagatcg atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc
    17101 tttacaagta tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct
    17161 gcagatgatg gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc
    17221 gttccaatca tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt
    17281 atgcaagaat taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt
    17341 gttgagatct cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta
    17401 gtcgcagaag tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc
    17461 gaagcacgtc ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca
    17521 cttcatgttg gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca
    17581 aacgatcttg gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc
    17641 ttgaatgatg tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca
    17701 cgtgcagcgg gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc
    17761 cgtgtgactt tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac
    17821 gtaatcatca aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag
    17881 attgatgtgg aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa
    17941 agtgatgtaa ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct
    18001 acaccgcagg caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc
    18061 atctataaag ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt
    18121 gaagaaaaaa tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt
    18181 acgattgcag gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta
    18241 atccgtgacg gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat
    18301 gacgtgaaag aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac
    18361 atcaaagtag atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa
    18421 ctgataaaag gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa
    18481 tcttaaaaga agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg
    18541 taacgatcac agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca
    18601 ttttatctga cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag
    18661 gattgattcg tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct
    18721 ttgaacttga tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga
    18781 acaaagacta atcaaaaact cccgcaaatc ctgtgaaatc aggggttgcg ggattttttt
    18841 gttttcatta agacgtatta cctaagagtt ccatgacttt agtcttgcgg cgtgtcagct
    18901 aaccaaaaaa gagaaaaaga aaagtaaaaa attcgacttt cttttttcta gtagtatata
    18961 aatttttgca tatagataaa gatcaagata ccgataacgg aagctagatt gtaatctatt
    19021 ttagtgacag tcaggtatag ccaagtaact agaagatcag tggaacctgc accataaaag
    19081 tttgagtttg tcggtccgtt gacagttaaa agaaaaatca cgttgaagtt attgatattt
    19141 ccgattacgc cgcttcgatt tggtcacgat ccaaattttg gataatccct gtagaaatta
    19201 gcatagtagc tgggatacca attcacatat tgacaaagat aacagagact tttacataaa
    19261 acggatcaat caggaaagga attgctcgat ccagccccag ttttccagca atgtgttaac
    19321 tggcccagcg tcattcagca aattatgcat gatgaggagc gaaatgaact gcggaacagc
    19381 cattgtgata acgacaagtt ttttggatca atgtaaaaaa cgtcagcgaa aggaaaaatt
    19441 tcgtggtttt ttatacctgt tcatttggct agatataaaa tccaaacaaa tgaacagaag
    19501 tgcttattag gtttcaattg ggagtatctg cgctagaata agagtaataa tgaaaaggag
    19561 tgttatgtat ggattattct agtaaagaat actttgataa aatgacggct tggtggcgtg
    19621 ctgctaatta tctttctgtt gggcaacttt atcttaaaga taacccctta ctgagacgta
    19681 cactgaaacc cgaagatgtc aaaaaacatc caatcggtca ttggggaacg attcctggcc
    19741 aaaattttat ttatgtccat ttgaaccgag tcatcaataa atatgatttg aacatgtttt
    19801 atattgaagg gccaggacac ggcggacaag tcatggtatc gaatgcttat ttagatggaa
    19861 gttacacaga gatctatcca gaagtaacgg aagatgagac aggaatgcaa aaattattca
    19921 aacgattttc atttcctgga ggcattgctt ctcatgcagc accagaaaca ccaggttcta
    19981 tccatgaagg cggagagctt ggatactctc tttcacatgc agtcggagct gtattagaca
    20041 atcctgaagt aatctctgct gtcgtgattg gtgatggtga agcagaaacc ggtcctttag
    20101 ctggaagctg gttctcaaat gtctttatca atccagtgat agatggagct gttttaccga
    20161 tccttcattt aaatggtgca aaaatagcca acccaacgat cttggcacgt aaatcagatg
    20221 gagaattagc aaattacttt aacggacttg gctgggaacc tttctttatt gaaggaaatg
    20281 attcagaaaa attaaatcct gttatggctg aaaaaatgga tcaagcaatc gagaaaatta
    20341 aatccatcca aaaagaagct cgcctgaaga cagcaacaga tgtcgtgatg ccaaaatggc
    20401 cagtattgat cgtacgcaca ccaaaaggat ggaccggtcc aaaagaatgg gacggggaac
    20461 caattgaagg aaccttccga gcacaccaag taccgattcc tgtagatcaa gaacatatgg
    20521 atcatgcaga tgctttactc cgttggctga aatcctatga accagaaaaa ttatttgatg
    20581 cacaaggacg tattttagaa gaaatcagag aaatcgcgcc tactggtgac caaagaatgg
    20641 caaaaaatcc tatcaccaat ggtggaatcg atccaagacc gcttattatg ccagattgga
    20701 aaaaatatac tttgcaattt gaaaaaccag gctccgttaa agcagaagat atgaccgaac
    20761 ttggtaaatt cgttcgcgag atcattgaga aaaatccaga aaatttccgt atatttggac
    20821 cagatgaaac aaaatcaaac cgattgaatc aagttttcaa aacgacaaat cgccaatgga
    20881 tggaaaaaat cgaaccagaa aatgatgaat ggctttcgcc atctggtcgc gtcatcgaca
    20941 gtcaattatc ggaacaccaa gatgaagggt tcttagaagg gtacgttctg actggacggc
    21001 atggtttttt gcaagttacg aatcattctt gcgagtggtt gactctatgt tgactcagca
    21061 cttcaaatgg atgcggaaat cacatgacct ttcttggcga aatgattatc cttcgctaaa
    21121 tctcattgca agctcaacag tcttccagca agatcacaat ggatatagcc accaagatcc
    21181 aggaatattg acacatttgg cagaaaagaa ggcggagttc atccgtgaat atctgcctgc
    21241 ggatgcaaat acattgctcg cagtcatgga taaagcattc cgctcgtcag aaaaaatcaa
    21301 tttgatcatc tcttccaaac atccgcgtgc tcaattttac tcagcagaag aagcagccgt
    21361 cttagtgaat gaaggcttga aaatcattga ttgggcttcg acagcaaaag aggaggaacc
    21421 tgaattggtc attgctgctg caggaacgga atctaactta gaagcattag cagctgtaac
    21481 tttgctgctg gaagaattcc caaaactaaa aattcggttc atcaatgtcg tagatttatt
    21541 gaaattacgt catccaagtc aagatccgcg tgggttgagc gatgaagaat ttgatcaata
    21601 ttttacaaaa gacaaaccga tcttatttgc tttccacggt tatgaaacat tggtccgcac
    21661 gattttcttt gatcgtcata accatcattt aatgattcat ggctacaaag aaaatggaga
    21721 tatcacgaca ccatttgaca tgcgtgtagt aaatgaactt gatcgttatc atttggcaaa
    21781 agacgcagct ttaaaaatca aaggaagtca agcagaagat tttgcgaaaa aaatggatca
    21841 gaaattacaa gaacatcaaa actatattcg tgaaaatggt attgatctgc cagaagtatt
    21901 ggattggaaa tggaaaaatc ttgaccaata aaatgataaa atcgatcccg caaaatagaa
    21961 ttttttacat tgattaagaa actgaaaaaa taaataaaaa aaacggaaaa aagactggaa
    22021 gaatggccag tctttttttt cgtttttggg aagtgaaaac caaactattt acctgtaagc
    22081 cacccataaa tccaatcaaa tggtgcaaag aaaaaatctt ccattctatt ccctccttta
    22141 aaaaagcata acatgatttc tatttacgtg agcatgaatt ttgttacgtt tttattgccg
    22201 aaaaataaaa accgttcatt atgatgtcaa attcgtcagt aattgatgca atgtgttata
    22261 ataattcagt taaatcaaaa atgaaatgga gaaaagacat ggacgggtta ttacctttat
    22321 ggaaagaacg cggaatgaca agtcatgatt gcgtattcaa attaagaaaa atcttacata
    22381 cgaaaaaaat cggacatggg ggtacgcttg acccagacgt agacggtgtg ttgcccattt
    22441 gtattggaaa agccacaaaa gttatcgaat atttgacaga ttctgggaaa atctatgtag
    22501 gcgagataac tctaggtttc agtacaacga cggaagatcg atccggagaa cgggtagaaa
    22561 caaaaccagt agatatgtct ttatctgaaa aaaaaataga tgaagtgatg gcttcattta
    22621 ttggtgagat cacccagata ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt
    22681 tatatgaata tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag gctcagattt
    22741 accgttttat ccggacaagt gaattgacaa gagacgaagt gacaggaacc atttcttggc
    22801 gcttcgaagt agaatgcggg aaaggaacct atgtccgcac attagctgta gatacaggca
    22861 agaaacttgg ttaccctgcg cacatgtctg atctgacccg aacagcaagt gccggtatgt
    22921 cccagaagga tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg
    22981 atgattgttt gctgccaata gaaaaaggag tggaacggtt caaacgagta gatattactg
    23041 atgaagtatg gcagaaagta aaaaacggta tgagacttga ttatactgta tttgcgctcc
    23101 cagaaatgcc gtcagaagaa attgctttgt tttataaagg aaaagttgtc agtatttatc
    23161 agccaaaccc gaaagaaaaa aacaaattaa aacctagtaa agtattgaga aatgaggaat
    23221 aacgaatgaa tatcatcaag atcagacatc cttaccaatc agaacaaatt ccagaagacg
    23281 atgtcgtttt agtgctggga tttttcgacg gcgtgcatat cggacaccag aaagtcataa
    23341 agacaggtat ggaaattgca caaaaagaag gattgaaatt agcattgatg actttcaatc
    23401 agcatccatc tattgtcttt aaaaagatcg atcctagcag cgtgaaatat ttgactagcc
    23461 ttaagcaaaa agaagaaaaa atggccgctt tgggtataga ttatttatat gaagttgatt
    23521 ttacttcttc ttttgctcat ttagcaccgc aggaatttgt tgatcaatac atcgtagggt
    23581 tacatgcaaa atatgctgtt tccggttttg attatacgta tggaccaaaa gacattgcag
    23641 atgtcgctca ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca gtttctaaag
    23701 aagaagaaca aggtgaaaaa atcagctcta cccgtattcg tggattacta gacagcggac
    23761 aagtaaaaga agcagcaaaa ctattaggtt gcacatatga ggtagaaggg gtagttgtcc
    23821 atggcgatgc acgaggcaga ctactaggat tccctacagc aaatgtcaaa gtaaagagca
    23881 cggttcattt gcctaaagaa ggtgtttatg tgtcggaaat aaaaattggg gaaacatggt
    23941 atccagccat gggttcaatt ggacataatg atacattcgg tgaaggacgg gagctgactg
    24001 tagagttgta tattttggac tttaatcaag atatctatgg tgaacatgtc gcggtacgct
    24061 ggaatcaatt tttaagagat caagtgaaat tttctagtgt agaggaattg attgaacaac
    24121 tcaaggagga tgaacaagca acagctgaat atttttctca gcaataaaac attatgcata
    24181 aaatctcttg tttatgtata actagcaatt acaataaaat gggtgataaa taagcccagg
    24241 aaaggggctg tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga
    24301 agtagcttct tcggaaataa gccgaaattt cttgaagctg accacttctg tcacaacctc
    24361 tttctttata gaaataaaag gacactagca attcatgaaa acgattgcta gagcgtatca
    24421 tttgctttag actatatatc ttggtgtaca ctcactttaa ggcggaaata aaagttatcc
    24481 gtaataacag gtttaggtat gaaaaaggag tgggtactat gtacaaggaa ttagaaaaca
    24541 aagtcgcagt agtaactggt ggctcaaagg ggattggaac agcgattgct caacgattcg
    24601 ggaaagaaaa aatgaatgta gttgtcaact ataactcgga taaagaagga gcagaaaaag
    24661 cagtcaaagc aatcgaagag gcaggcggaa aagcagtctc tgtacaagca gatgtcagtt
    24721 ctgaagaagg aatacagcgt ttacttgata cggctttaga aaattttggg acactggacg
    24781 tttgggttaa taatgctggg atggaaaatc agcatccaac ccatgaactt agccttgaag
    24841 attgggaaag agtgttaaaa gtcaatttga ctggcgtttt tatcggaacg aaagcggctt
    24901 taaattactt tacagagaat aataaaaaag gaaatatcat caatatgtct tctgttcatg
    24961 aacagatccc atggccaacg tttgctcatt acgctgcttc aaaaggcggg gtaaaattat
    25021 ttaccgaaac ggtagcaatg gagtatgcac cgaagggaat cagaatcaat agtataggcc
    25081 ctggtgctat ccgtacacct attaatgcgg caaaatttga tgatcctgaa caaaaagaac
    25141 tcttggaaag tatgatccca ctaggacgcg taggtgaacc aaaagaaata gcggcgacag
    25201 ctgcatggtt ggcttcagac gaatccagtt atgttacagg tattacacta tttgcagatg
    25261 gtgggatgac tttatatcct tctttccaag gtggaaaagg ataagggtga ataaaaatgg
    25321 gtttattgat tgcattgatc ccggcagttg gctgggggat ccagccactg attttgaaaa
    25381 agattggtgg cagaccaaca aatgagatat taggaactgg tattggagcg gtgcttgtcg
    25441 gcttactcgt tcaattattc gcttctccag ctggtatttc agtgactaca tttttgatta
    25501 gtttactttc tggtgcattt tgggttattg gacaaacagg acagtataca tctttcaact
    25561 tgattggtgt ttctaaaacg atgccaattt cgactgcatt acagttagtc ggaacctcgt
    25621 tgattggtgt cttcgctttt ggagaatggt ctggaagtat ggggaaaatc attggagcag
    25681 cagcaatcat tcttcttgta tttggttctg ctttaacggc aatcagtgat gatcctggaa
    25741 aaaaaggcgg tatcacaaaa ggaattagtc tattagcatc aacaagtatt ggttattggg
    25801 tatacagtgc attgcctaaa ctagttgatg caagcggact ttccattttc tttccgcaaa
    25861 tgatcggtgt ctttttaggg gcagtgattt atgtgatttt ccgtaaacca accgcatttt
    25921 ctgaagacaa gagttggaaa gcaacgatcg ttggagccat ctttagtgtg tcagcattgg
    25981 cttatatttt ttcagcaaat gaaaatgggg ttgctactgc atatatcatt acacagctaa
    26041 acgtagttat ctctacatta ggcggtatgt acatcctgca tgaaaataag acaccaaaag
    26101 aaaaaaaatt aacgattatc ggattattgc tgatcgtagt aggaagcatg gtcacggtct
    26161 ttatttgagg ataaagatgg taaaatttca tttattaaaa aaatatttgt ttgaaccaaa
    26221 cagtaagtac ttataaaaaa atagcgctag aatttccaac atctctggaa tttctagcgc
    26281 tttttaattg tttaagatac aagggaaata tctgtctgga tcaataattt ttcattgatc
    26341 cattgactaa agtttacgac ataattgaag ttaccaccga ttagtatctc cgcatgattg
    26401 tctttataaa gatcgtattg taaaagacgg tcttttttat ttgcgtaaac atcgttatgg
    26461 tactggacta attgttctcg gaattgattg attgctgtaa aatcacgata ttgaaggttg
    26521 aaacgcatcg tgattggtgc atttagcatg aatcgctgca tttcttttgc tgcttcctct
    26581 gcttgatctc cgtataggaa ctcaaaaaga ttacgttcag aaaatttcca ttgcccgcca
    26641 acctttgttc cttttagttt tccttcatga agataatttc tgatagaacg tgtagtaaca
    26701 cctaattgtt cggctaattc atttactgag taaaaatctt tcaatgttca ccctcctatg
    26761 tgaatggttc tactgactta gttgatattt gcttaatcac gaaatgtaga aagaacgatt
    26821 ttaattaagg tcattgtaaa aatcaagtca gaaaattcta acttttattt ctctaaagaa
    26881 attcgttctc tcctaaagtg atattatatt taaatatacc tgaaaataat gggcagtatt
    26941 atgaaagatc actgatattt tctcgtattt tttccttatt caaaagtttt gcaggaaaat
    27001 tgctgaaaaa ataattttac tatgtagaaa attcaaagcc attcaatcag tgattttttt
    27061 cagcacaaga aagactcctt ataaaaatag cagatacttt gtcaattaag tgtttaacca
    27121 gggatgataa aaaggatagg attgaacaaa acctgtataa ggtgatgaat agtttcctcg
    27181 ccagtaaaag gtttattcgt ataaagcttt atcgccaaaa tttatcatga ccttttttat
    27241 cttcaatatt aaatgaaatc aattgttcaa tcaattccca gttgattaca cttttccaag
    27301 gaactttgat gacgttatcc gtatatacgt agcctgcttc ttggatattt ttttcaaggg
    27361 cacgaatcgc tgctttttcc ggtgcgatag aaaaatgatt tttagcaaaa ctaaatgcaa
    27421 taataaaggt accatggtca gtaaacatag gttggttcca ttttacagtt gtgtctagct
    27481 ggggatacgt ttctgttatc ttagcaaaaa ggtctttgat cttttgttga tgttcttttt
    27541 ctttgatttg attgatgatc ggctgaaatt tttccattct aaaacgcctc cattaagtgg
    27601 tttgatttat tttaccagaa aaacaatagt tatgtataaa aaacgaacaa aacacaagta
    27661 atgttgtaac cgctatattt atatgataag atgtctcaaa taataaaata gaaaagagac
    27721 ataaaaatga ttgacttggc aaagaaattc ttacacgaaa atatatattt tgagttgtat
    27781 catcatcgag ccatttatac aaatgaagat gctttggtaa taaagaaaga acaaggattt
    27841 acaggtacag agaccaaaag cttattctta aaagataaaa aagataataa ctacgtatat
    27901 ctgactttta ctacgaaaca aacggatttc aaagcattag ggaagatcgt cggtaaaagg
    27961 ctttctattg tttcatcaga acgcatggaa gaacaaacgg gacaaaagca gggcgctgtt
    28021 tctccgtttg gttatgaaaa acagattccg attattattg atgaagagct cttatcaaat
    28081 gaaaaacttg tttttgcacc aggtagacca gatcaaacga tggtcatcca aacggttgac
    28141 ttaaacaaaa tcatccagct acttgagatg ccgatatttt tgatgccggc aaacgaaaaa
    28201 aacaattaac caagtaaaat agtgcatact tggaggtgaa gcaacatgat aagttatcca
    28261 ttattagaaa gaaatgcaac gattggggtt actgcaccat cctcaggcgt atcagaggaa
    28321 caccattcgt tactcgaatc agctttaaag agaatggaag aaaaatcttt ccaagtagtt
    28381 actggaaaga cgctttggac gcaagataag gaaaaatcag gtccagcaag aaaacgagca
    28441 gatgaattca atcaaatgat gcaagatgaa aatatccaaa tgatctttcc gacttgggga
    28501 ggagaattat tgattgagat tctcgatttt gtcgattttg atgctctaca accaaaatgg
    28561 atagtaggtt attcagatat tagtacgttg ctacttgctg tcacattaaa aacagggata
    28621 gccactgcac atggaactaa ccttattgat ttaagaggag agtacttgga tgaaacgaca
    28681 gcaatgtggg aaagtgtact gaaaacaaga gaaggagaat cgattctaca gcaatcttct
    28741 gaaaagtatc aaaaagtgtg gccccatgag cattcatcat cttctgtttt ccatctcact
    28801 gaaccaacaa attggcaatc agtcagcgga gatgcagtaa ggatgaaggg acgattatta
    28861 ggtggttgta ttgatacgat caggcattta atcggtactc cttatggaga tgtccaatct
    28921 tttaaaagaa agtataatga tggaaagccg atcttgtggt ttctagaaaa ctgtgagcta
    28981 tcgtcagttg atttaagaag atcacttgtt cagatgaaac tggcaggctg gtttgatggc
    29041 agctcgggga tcatgttcgg tagaagtgcg gcagaggatt tgttagatta taaaatgaag
    29101 gatgtatacg aagatctgtc aaaggaatta aaactaccga tcttgtatga tattgattgt
    29161 ggtcatgtac cgccacaact gacattgatt aatggtgcct atgcagaagt agcggtagaa
    29221 gatagaaaag cgactgtttt gcaaacgttt atttaaaaaa ttttttcctg ccttggagtc
    29281 tatcaaagac caataaatct aaatagaaaa gaaagtgaaa gaagctgaca aaatgctgtc
    29341 agcttctttt tgtataacat attagaaagt ctaattatct ataactgtca catcgattaa
    29401 ctgtttcacg tcattgggat ttcctgtagg aatagctact acagatgcat tatcaatgat
    29461 ttcttgagtt gtttctctaa tgaacgctgg ataagatgtt tgatctgcag gcatttgcca
    29521 atgtgaagga actggataac tagtgattgt cccgtcatgg ttacttgaat acactaaaac
    29581 tccctggtaa ctgtatttcc cagtgagcat aaccacatct ttcggataga ctgcactccc
    29641 tgcatcataa ggggcgatag gtgttcctgc aggataatga ataacatgta attcgaaatc
    29701 acccgatgta tctctaaaag tattacctct aatagctagc caaacacgtg cgtactcaat
    29761 ttgtagatca gaatatcctg ctaacggatc tattgactga gaagactgtg aggaggttgt
    29821 gtctattgtt gaagaagaaa tatttgtctc aactgtacta ctagatgtag aagaagtatt
    29881 gggctctgag actttactgc ttgttgaaga tgtattcgtt tcagtagaag ctgatttctg
    29941 agtacttgat ttttcagctg aactggtttg tctgattgaa cttttgtctg tggtcgcaga
    30001 tggatcacta ttttctttac taggagacgt acatcctaca aggaataaag agaccaacaa
    30061 taaaataaaa cctaactttt tcatataaaa tacctcatta tttttttaat accagtatag
    30121 cataatcctg aatttttgaa tccgtaaaac cttgtctatg aatataaata gagtgtatct
    30181 tctttgataa atgtcagaaa aagtgtgaca aataatcatc agtaaaatta cttgtcacac
    30241 tattgataag gagtatttaa ttgaagagaa tgattaggag aaaggatctc cgtacttttt
    30301 atagtaatca tagaatccaa gtttgtatag acgaatcgtt ttttctctta tttcttctgt
    30361 ttcaaaatgg actgcttgaa gaatcaagtc tgtgaattct ataggagctg ttccattcgc
    30421 agtaatcaaa ttacgatctt tcacagcttg ttgttctata aaatcttctt tattctgata
    30481 attttccgat tctaaccaag gaaattgcgc atttccggtg tgtttgtaac cttgcaataa
    30541 tccgctctga gctaaataat caactgcccc acaaatagca gctactggta cgtctgtttt
    30601 taacaattta tcgattattt gatataaatg atgatttttt atgtcccatg aattgccacc
    30661 gatcataata aaaaggtcaa ctttttcagg tatttgttcg attaagtaat cgatttttgt
    30721 ttttaatcct ccaatggaag tgacctctga ttggatagaa gcagttttgt tttgccattt
    30781 ctcattttgg tttagtgtgc tagtaatata agcaccttcc caatcggcat attcctctaa
    30841 tagaaacgta atcgcagttt tcattttcaa gctctccttt actatctata acaaggacaa
    30901 cagttttaac ctacagacat tcattaaaat tgggtagcga cagcgaaaac aacaattctt
    30961 aagttttaga cagagtatac agatagtcaa cttaattaaa tacgaactaa ataaaaatga
    31021 caggtaatca tttttgttta ccagcattat aacattttgc atacatggat gacaaaagaa
    31081 ccaattccca cttgcttgag tacaatatta aaagaaactg gaaggcaaaa agtaattata
    31141 gaaacgaaaa ataaatcata tcgggcactc agacatgaaa acaaccctag agatatataa
    31201 ctaggtaacc agtttaatga aagaaaaaat catccaagaa gtcgattctt ggatttttta
    31261 gtggatttgt tcgccaaata cgtttagtat gcgtatatga gaggttgtaa gcaaagcgtt
    31321 gtggtctgag cacttaagga ggaatctgtg aaaatagctt ttcatatttt tgcagatttt
    31381 gacttaatgc cgaaggtcag ctttgcgatt accgtttatc gagaggctgt aaaagcaccg
    31441 tgtagctccg atgacaataa gcaagcattt ttagagctag gtttccttga ttctaaatat
    31501 gcggcttatt tcaatgagtt ggtgcttgaa cgcgatttta agaaggtcga aaacaggcac
    31561 gacttttcga cgtaaggaga aagaatttca atgatagaaa caataaatcc cgcagaaaac
    31621 aaggtttctg cgtacattca tatcccattt tgcgagcata tctgctatta ctgtgatttt
    31681 aataaagttt ttttagaagg gcagccagta gatgagtatg tggaaatgct gctgaatgaa
    31741 atgaaaataa tgctagaaag tcatccgaca gcgaatttag aaacacttta cgtagggggc
    31801 gggacaccaa cttctttatc tgctaaacaa cttgatcgac tgttatcagg tgctagagag
    31861 atcctgcctt ttcaaaatgg taatgagttt actgtagaag cgaatccagg tgatttgacc
    31921 cgtgaaaaac ttcaagtaat gaaaaactat ggcgtcaatc gtttatccat gggagtgcag
    31981 acatttgata atcgcttatt gaaaaaaatt ggacgtaaac acaccgcaga agatgtgtat
    32041 caaactatgc aatttttaga agcagaaaat tttaccaatg taagcatcga tttgatttat
    32101 gcattaccgg gacaaacact agaaggatat ctggatactt tggaaaaagc tttggctttg
    32161 gatctgcctc actattcttt gtactcattg atcctcgaaa acaaaacaat gttcatgaac
    32221 tgggtacgac aaggaagact cgaactgcct gatcaggaaa cggaaaccag aatgtttgaa
    32281 gagacgattg aagcaatgga aaagcatgga cgtcaccaat atgaaataag taactttggg
    32341 ctctccggac atgaatctca acataacctc atgtactgga acaatgacca ctattttggg
    32401 tttggagctg gtgctagcgg ttatcttgga gatattcgtt accgcaacaa aggaccaatc
    32461 caacactatt tacgcccatt acgagcaaat gaacgcccgg tattggaaga agaaaaattg
    32521 acacgaaaaa atcaaataga agaagaaatg ttcttaggac taagaaaaaa aacagggatt
    32581 actaaaaaac atttctatga tcgctatcaa aaaacaattg aatcggtgta cggtgatgtg
    32641 ctaagggaat tagagcaaga agggttactt gttaatgaag aagaccgtgt ttatttgaca
    32701 ccaagaggga cctttttggg gaacaatgtg tttgagcgct ttttgtttga aaaatagttt
    32761 aaagaatatt tgttttttat ctgaatttaa ataaaaaaag ttcaaaaata caataaagga
    32821 ctattgaaac gttgatataa ctggatttac atcaattatt tagaattagc actcttgtgt
    32881 ttagagtgct aaaaaatgga gtctttctct tgacaggaga agggcttctt gctatattta
    32941 taagtggatt agcacttaga atatatgagt gctaatgaga ggtgaggaag atgttaacaa
    33001 agcgacaaaa cgacatttta cgtctgatca tccaacacta tacgagtagt ggcgtgcccg
    33061 ttggttccaa aacattgatg tctgagggag tcaaggccag ctcagcaacg attcgcaatg
    33121 atatgaaagc attagaagat gaaggattgc tgttgaagac tcactcttct tctgggcgga
    33181 ttccatctgc tttaggctat cgttattacg tggatcattt gttaaaacct gctcgcgtag
    33241 cagaagatga tttgctgctg attcgtcagt ctctaggcag agaatttcat gaaatcaatg
    33301 agattattaa gcaatctgct gagatattat ctgagctgac aagctatacg actttttcat
    33361 tgggacctga aataaaagac cgccgactga ctggctttcg tatcgtaccc ttgaataatc
    33421 gacagattat tgctatcgtc gtaacagata tggggaatgt agaaagccaa gtgttcactc
    33481 ttcccgaata tatgggaagt caggaactag aaaaaatggt acagattgta aacgaccgtc
    33541 tagtcggaga tcctctgatg acggtgtacc ataaacttcg tacggagatt ccgatgattc
    33601 ttcataaata tttccaaacg acagaaggga tgtctcagtt atttgacacc gttcttggtc
    33661 atgtttttga agataaggtt tatgtcagtg gacaaatgaa tctcttggat tatgagcccc
    33721 gtcaagatct tgatcagttc aaatcgatgt tttcttttat gaaagactca gacggactta
    33781 cacaaatgat cactccgatg gatagcaata ttcatatcaa gattggtgct gaattaggca
    33841 atgatttatt gcagaatatg agtatgattc aagcaagtta cgagatagat ggtcatggaa
    33901 gaggtacaat agccttgtta gggcctacta gtatgcctta ttctcggatg ttcggtctag
    33961 tcgatgttta tcgaagagaa ctagcagcta aacttgctga ttactaccgt tcgcttgact
    34021 tgtcaaactc gtaatgtgaa taacgaagct atttgcagct gatcgttctc tctttcttag
    34081 taagagcaat caaacagctg actgaataat gaaaggaaga ctgtatcgtg aaagaaaatc
    34141 aagaagaact agataaagaa atgacagatg ctcagccaga accggaaatc gatgtcgaag
    34201 ctgcagaaga ttcgggtatt tcagaagctg aagcggaaga attcgaaaca gcgaaattaa
    34261 aagctgagtt agaagaaatg gaagataaat acttgcgggc tcgtgctgaa atcgcaaata
    34321 tggcaaatcg aggaaaaaat gagcgtgagc aattacaaaa ataccgttca caagatttag
    34381 ccaaaaaatt acttccatca attgacaacc tggaacgtgc attagccaca gaagtgtcag
    34441 atgatcaagg ggcaggtttg aaaaaaggcg tggaaatggt cttggaaagt ttgcgtaacg
    34501 cactggaaga agaaggaatt gaaaaaatcc ctgctaaagg agaagccttt gatcctaatc
    34561 ttcatcaagc agtacaaact gttccagcga ctgaagatac accagccgat acgattgtag
    34621 aagtactaca agaaggctac aaattacatg atcgcgtgtt aagaccaacc atggtcattg
    34681 ttgcacaata attgtgcgga tataaaactt acactttcaa ttaaagaagg agacgttaat
    34741 tatgagtaaa attatcggta ttgacttagg tacgactaac tctgcagtag cagtattgga
    34801 aggaaacgaa gcaaaaatca tcgctaaccc agaaggaaac cgtacaacac cttctgttgt
    34861 atcattcaaa aatggagaaa tccaagtagg ggaagttgca aaacgtcaag ctgtgacaaa
    34921 cccaaatacg atttcatcaa tcaaacgcca tatcggtgaa gcaggttaca aagtagatgt
    34981 agaaggtaaa tcatatacac cacaagaaat ctcagcaatg atccttcaat atatcaaagg
    35041 attcgcagaa gactatttag gtgaaaaagt cgaaaaagca gttatcactg tgccagctta
    35101 ctttaatgat gcacaacgtc aagcaacaaa agatgctggt aagatcgctg gtctagaagt
    35161 agaacgtatc gtcaatgaac caactgcagc tgctcttgcc tatggtttag ataaaacaga
    35221 tcgcgacgaa aaaatcttag tattcgacct tggtggcggt acttttgacg tatctatcct
    35281 tgaactagga gacggtgtct ttgatgtatt gtcaacagct ggcgacaacc atctaggtgg
    35341 ggacgacttt gataacaaaa tcatcgacta catggtagca gaattcaaaa aagaaaacag
    35401 cattgacttg tctcaagaca aaatggcttt acaacgcttg aaagacgctg ctgaaaaagc
    35461 gaaaaaagat ttatctggcg tatcaagcac acaaatcagc ttgccattta tcacagcagg
    35521 agaatctgga ccattgcatt tggaaatgac attgacacgt gcaaaatttg atgaattaac
    35581 agctgactta gtagaacgta caaaagtacc agtacgtcaa gcgctaaaag atgcaggttt
    35641 atcacaatca gaaatcgacg aagttatttt agtcggtgga tctactcgta tccctgcagt
    35701 tgtagaagca gtacgtaaag aaacaggaaa agaaccaaac aaatcagtta accctgatga
    35761 agtagttgct atgggtgctg ctatccaagg tggcgtgatc actggtgatg tgaaagatgt
    35821 tgttttactt gacgttacac cattgtcatt aggtatcgaa acaatgggtg gagtgttcac
    35881 taaattgatt gaccgcaata caacgattcc aacaagtaaa tcacaagtat tctcaactgc
    35941 ggcagataat caaccagcag tagacatcca tgtattacaa ggtgaacgcc caatggctgc
    36001 tgacaacaaa actctaggaa gattccaatt gacagatatt ccagcagctc ctcgtggtat
    36061 tccacaaatc gaagtaacat tcgatattga taaaaacggg attgtaaatg tatctgcgaa
    36121 agatctaggt acacaaaaag aacaaaaaat cacgatcaaa tcttcttcag gtctaacaga
    36181 cgaagaaatc gaacgtatgg taaaagatgc agaagcaaat gcagaagcag ataaagctcg
    36241 taaagaggaa gttgacctac gtaatgatgt agatgcattg ctattctctg tggacaaaac
    36301 attgaaagaa ttagaaggca aagtggacga agaagaagtg aaaaaagcag aaacagctcg
    36361 cgatgaattg aaagccgctg ttgaagcaaa caacatcgaa gaaatgaaaa ctaaacgcga
    36421 tgcattgaac gaaatcgttc aaaacttgac tgttaaacta tacgaacaag cggcacaaca
    36481 acaagctcaa gaaaacccag aagcagctca aggcggagca gatgatgtcg ttgatgctga
    36541 ttttgaagaa gtcgatggcg acgataaata atcagttcac tgaatttcga gagaaggcca
    36601 aagttgctac tttggctttt tcttttgaaa aatagtttga agtatcggtt tttgtttcaa
    36661 acaaaatatg atatgataac aagcgacgtg aaaatgatga gaaccaatag gaggaaagcc
    36721 tatggcaaca aaacgtgatt attatgaagt cttagggctc tcaaaaggag catcagaaga
    36781 tgaaataaag aaagcttatc gtaagctttc gaaaaaatac catcctgaca tcaataaaga
    36841 acctgatgcg gaagaaaaat tcaaagaagt atcagaagca tatgagattt taagtgaccc
    36901 gcaaaaacgc gctgcatatg accaatacgg gcatgcaggc acagatccaa attacggtgc
    36961 tggtggagct ggtggcggat ttggtggatt cggcggcttc tctggtggcg gcttcggtgg
    37021 ttttgaagat atcttcgact ctttctttgg aggaggcggt cggtcagtcg atccaaatgc
    37081 gcctcgtcaa ggtgctgatt tgcaatatac ggtcgacttg tcttttgaag aagcgatttt
    37141 tggtgtagaa aaagaaatca agtataatcg ggaagaaatc tgtcatactt gtggtgggaa
    37201 cggtgcaaaa ccaggcacac aaccaactac ttgtcataaa tgtcatggtg caggtacgat
    37261 taatgtagaa cgccaaacac cacttggacg agtgatgagc cgtcaaactt gtgatgtttg
    37321 tcatgggaca gggaaagaaa tcaaagaacc ttgtccaacc tgtcatggct caggacatga
    37381 gaagaaagca cattcagtta aagtgaatgt accagctggt gtagaagaag gacaacaaat
    37441 gcgtttagct ggtcaaggag aagctggaga aaacggcgga ccatttggtg atttgtatgt
    37501 ggtcttccgt gtagaagaaa gtgatatctt tgatcgtgaa ggttctgaaa tttactatga
    37561 attgccttta agttttgtac aagcagcttt gggggatgaa gtccaagtac caactgtcca
    37621 tggagatgtg aaattgaaga tccctgctgg aacacagaca ggtacgaact tccgtttacg
    37681 tggaaaaggc gctccgaagt tacgtggtgg tgcaacaggc gaccaacatg tcaaagtaaa
    37741 actgatcaca ccgaaaaatc taaatgaaca acaaagagaa gcattacgtg cattcgctga
    37801 agctggcggc atcaaagttg aagaacagca agaagacggt ttcttcgata agtttaaaga
    37861 tgcttttggc gggaaaaagc ggaagtagtt tttctagtaa aaacgctaag tcattaataa
    37921 ttaaaaacca gacgaacaat catatgttcg cctggttttt ttgtagttgt catgtttgta
    37981 ccaacaaaaa agccagcttt agaatatgat aatcgtcata gtttctaggg gtataaccag
    38041 tgagagaatg gaacttgttc aatcgatatt gcaaggtgtt tttatgaata aataattctt
    38101 ctgagcaatt tttgatgctt ccgttatttt tactgaagca tgccaaagtc ttcttgaaca
    38161 aatgtacttc ttcttctgtc aggcttttca aaatacgttt tacatataat gttcgttgat
    38221 ctgacgggat tgctggtaaa acgatcccga ggtctaaatc ttcaaaaaag cggatctcgt
    38281 tcacagattg atggaaagct atccattcgc aaacttgttt ggcatgaagg aaagattgcg
    38341 gtgtctcttg ctctgtataa caaagcgacc caattccaaa gtttagctgt aactgataca
    38401 ttttattaaa ctttgtctgg attttatcaa gcaatgatag caatgaggct ctggaaatcc
    38461 gattgactag gataatgatt tgttgtctat gtacagtgag caaagcctgt ccgtaataag
    38521 agaagaccct ttcgagcaac tccaataaag gattgatccg ttcttgacac aattgctgat
    38581 tgtcagaaag ttgtcccaca actacataat atggagaaga aaaatcaagc tcaattaagt
    38641 gagggtggta tcgactatgt acttgatatc atcaatgaac cgatggtact ttcttatgcg
    38701 atggaacagg tagagcgatt gcttgtttgt gcaggaaaac aagcgatgcg gctgatacga
    38761 ttaggtgaga aaataagaag ataaaaatac acggtccctt catggcgacc gtgcattttt
    38821 ggtttttatt aaatatttaa tactttattc aagaaatctt ttgtccgttc attttgcgga
    38881 ttttcaaaga tttgttgagg cgttccatct tctaaaaagt ttccgtcgtc aatgaacatg
    38941 acgcgattgg ccacttcttt agcgaacccc atttcgtgcg taacgataac catcgtcata
    39001 ccttgttctg ctaacttttt catgacgttc aagacatcac cgaccatttc aggatcgagt
    39061 gcagaagtag gctcatcaaa aagcatgatg tcagggttca tcgctagagc acgagcaata
    39121 gccacacgct gtttttgtcc accagaaagc atttcaggat acatatcttt tttatcttct
    39181 agaccaactg tctgcagtaa tttgattgct tttttctctg cttcttctct agattctttc
    39241 ttcaggtcca tcggtgctaa aatgatgttt tccaaaatag aaagatgagg gaataagtta
    39301 aagtgttgga ataccattcc gatatgttgt ctcaccaagt tgatatctgt gcttttatcc
    39361 atcaagtggg cgccgtcaat aataatttca ccgtcagaag gttcttctaa acgattcaag
    39421 caacgcaaga atgtactttt tcctgaacct gaagggccaa tgatacagac gacatctcct
    39481 tcgttgatgg atacattgat gtcgtttaat actgtgttat cgccgtattt tttgactaaa
    39541 tgctcaacta agattttttc tgccatctta cttcaccttc ttttctaaaa cttttgctaa
    39601 ttttgtcaat gctgtgatca aaatcaagta catgatcgca atgatcaagt aaacatacgt
    39661 actttgtgtt gtacgtgcaa caatgatctt accagtttgc agcaattcga ctacaccgat
    39721 tgcggaaatg atcgttgtgt cttttaatga gataacgaac tgattgacga atgatgggat
    39781 catgatgcgt atcgcttgcg gcaaaatgat tttttgcatt gtccgattat aagaaagacc
    39841 taaacttctg gaagcttcca tttgtccgac aggaacggcg ttgatcccgc ctcgaacaat
    39901 ctctgcgata taagcacttg cgttaagagt caaggtgata acaccagcaa ggaagtcagg
    39961 aatcgtaaat ccaacgatac caggcaaccc aaagaaaata aagaacgcaa gaaccatcat
    40021 tggaattcct cggatgatat cgacataaat actagcaatt gtgcgaagag cgcgtattgg
    40081 tgctacactg aataatccaa aaatcacacc gattactata gccaaagcaa aggagatcaa
    40141 agttaggaga attgttctcc aaagtccttt caacaatgct tgatagttgt tttggataag
    40201 tccaagaaat gttgattcat cagcagtgga agactcactg gaagtttctg aaccatccgc
    40261 tacatattct ttaataatct tatcgtattc gcctgttctt tttaattctt tcaatccttc
    40321 attgaacata ttaagaagct caggattttg tcctttttta acggcaaatg catattttcc
    40381 gccagattct tttttgatcg gtgtttccaa ttcttgtcct tgtttgatcg cataaccgat
    40441 taccggataa tcatccatca ttgcatcgat tgcgccaact tttaacccgt cgtacaattg
    40501 atcagcagca tcaaaatatt tgatagaata gccatattca gattcatggt cgcttaaaaa
    40561 ttcagcactt tcggtaccaa cttttgctcc gactgttttt cctttcaaat cttcataaga
    40621 cttgatcgaa gtatctcctt ttttgacagc taattggatc ccgctatcaa agtaagaatc
    40681 agaaaaatca tagcttttct ttcgttcatc tgttacagtc attccggcaa tcattccgtc
    40741 tgcttgtcct gattccaaag cttgcaaagc agcagagaaa ccacggtagt ccattcggat
    40801 gttaaatcct tgcagtttgg cgatgttttc catcaatttt acatcgatgc cttcgtactc
    40861 gccctttgcg ttttgaaatt caaatggagc aaaagcagag tcactggcaa ttacgtatac
    40921 gtcttttttg ggctctattt tttgcattgt ctcgcttgtg cttgactctg ccgttgtagt
    40981 gttgtcagct tgtgcaacat gaccaaacgc aaatgtcaca agtcctgtca ttaagaaaac
    41041 aaatgagtga aataaatgct ttcttttcat aatcaacctc ctaaatttcc aatattttat
    41101 tattataggc gaaaagtgct gaatcagaca agactgtaag ctcgaaaaaa tgaaaaaaat
    41161 gcgagtttct aacattttat gttataaaac ataacatgta taataagata aaaaagtggc
    41221 gaaagtatgt tcggtgtggt ataatttttt agtaaggaat gcctttgctt tccttttctt
    41281 tttattggtc gtaaaatgaa aaggaatgaa tgaaaaggag gacgaagaat gattgagaga
    41341 gatacctccc gacactttga gcttgtctcg aaataccaac cggctggtga tcagcctgaa
    41401 gcgattaacc aattagtgga tggcgtagtc ggcgggaaaa aagcacaaat cttattaggt
    41461 gcgactggaa cagggaaaac atatacgatt tccaatctta ttgagaaagt gaataaacca
    41521 acactgatta ttgcccataa caaaacattg gctggtcagc tgtacgggga attcaaggaa
    41581 tttttcccta acaatgctgt tgaatatttt gtttcttatt atgattatta tcagcctgaa
    41641 gcttatgtgc cttcaagcga tacgtatatc gaaaaagatt caagtgtaaa tgatgagatc
    41701 gacaaattac gacattcggc tactagttca ttattagaaa gaaatgatgt gatcgtcatt
    41761 gcatccgttt catgtatttt tggattaggc tcgccttttg agtaccaaaa acaagtagtt
    41821 tcgattcgac agggagcaga actagatcgc aatcagttga ttcgtgactt agtcagtatc
    41881 caattcgaaa gaaatgatat agattttcag cgcggtcgtt ttcgagtgcg cggtgatgtc
    41941 gtggagattt ttccggcttc aagagatgaa cgtgccttac gtgtggagtt ttttggagat
    42001 gaggtcgaac gtatccgaga agtcaatgca ctgacaggtg aagttttagg agaaacggaa
    42061 cacgtagcaa tcttcccggc gactcacttt gtgacgaatg atgaacatat ggagcatgcc
    42121 gtagcgaata tcaaagcgga attagaacag cgtttaactg tcttgcgaaa tgaaaacaaa
    42181 cttctggaag ctcaacgttt agaacagcga accaattacg atattgaaat gatgttagaa
    42241 atgggctaca cgtcgggaat tgaaaattat tctcggcata tggacggacg taaagaaggt
    42301 gaaccgcctt atacgctatt ggatttcttt cctgaagatt ttttgatcgt ggcggacgaa
    42361 tcgcacgtaa ccatgcctca aatcagagga atgtataatg gtgaccgagc gagaaaacag
    42421 atgctagtag attacggttt ccgtttgcca tctgctttag acaatcggcc acttcggtta
    42481 gaagagtttg aaaaacatgt caatcagatc atttatgtct ctgcgacgcc aggaccatat
    42541 gaacatgaac aaacagatac cgttatccaa caaatcatcc gtccgacagg gctgcttgac
    42601 ccagtgatcg aagttcgtcc aatcatggga caaatcgatg atctagtagg cgaaataaat
    42661 gaacgagtcg aaaaagacca gcgggtgttt gtaacgacat tgacgaaaaa aatggcagaa
    42721 gacctcacag attatttcaa agaattaggg atcaaagtca aatatttgca tagtgatatc
    42781 aaaacgctgg aacgaacaga aatcattcga gatcttcgat taggtgaatt tgatgtgtta
    42841 gttggaatca atttgcttcg tgaaggatta gacgtacctg aagtttccct tgttgctatc
    42901 ttggatgcgg ataaagaagg ctttttacgt agtgaacgtt ccttagtcca aacgattgga
    42961 cgagctgctc ggaatgaaga aggtaaagta atcatgtacg cagacaaagt aacagattct
    43021 atgcgtctag cgatggatga aacgtcaaga cgtcgtacga ttcagcagaa gtacaatgaa
    43081 gaacatggga tcgttccaaa aacgatcatt aaagaaatcc gagatttgat ttctattacg
    43141 aaagaatcgg aagatgatac aaaagaagcg gtacaagtct cgtacgaaga aatgacgaag
    43201 gaagaaaaag acacgttatt gatgaaatta gaaaaagaaa tgaaagatgc ggcaaaagct
    43261 ttggattttg aaacagccgc gaatgtgcga gatatgattt tagaattaaa agcttcaaag
    43321 taggaggaca acatggctaa cgataaaatt acgattcatg gtgcacgtgc ccataacttg
    43381 aaaaatatag atgtaacgat tccacgagat aaattcgtgg tcgttacagg tttgtccggt
    43441 tctggaaaaa gttccctagc atttgatacg ctgtacgctg aagggcagcg gagatacgta
    43501 gagagccttt ctgcttatgc tcgtcagttt ttaggacaaa tggataagcc ggacgtagat
    43561 agcatcgacg gattgagtcc ggcgatttct attgatcaga aaacaacaag taaaaatcct
    43621 cggtcgacag taggtactgt gacggaaatc aatgactatt tacgcttgtt gtttgcacgt
    43681 gtaggacatc cgatttgtcc caacgatcat atcgagatta ccagtcaatc agtagatcaa
    43741 atggcagatc aagtaatggc tttgcctgag cgcacaaaga tccaagtgtt ggcaccgatc
    43801 gtggtaaaga aaaaaggaca acataaaaaa atattcgaac gaatccaaaa agagggatac
    43861 gtccgcgttc gagtcgatgg tgagatctat gatttaagtg aagtgcctga gctggagaaa
    43921 aacaaaaaac atgatatcgc gatcgtcatc gaccggatca ttgtgaaaga agggattcgt
    43981 tctcgcctat ttgattcttt agaggcggcc ctgcgattag cagacggcta tgctctggtt
    44041 gacgtgatcg gtgaagaaga aatgttgttt agcgaacact atgcctgtcc ttattgcgga
    44101 tttactgttg gcgaactaga accaagactg ttctcattca atgcgccttt tggagcttgt
    44161 ccagactgtg atggtttagg tgtcaaatta gaagtcgatc aagatttagt gattccagat
    44221 agaacgaaga cacttagtca aggtgctatc gttccatgga atccaatcag ctctcagtat
    44281 tatcctcaaa tgttagcgca ggcttgtgaa agtttcggta tcgatttaga tacaccattc
    44341 gaagaattac cggaagagca tcaggagatc atcttgaatg ggacagatga tcttttccat
    44401 ttccattatg aaaatgattt tggtggcgtt cgcgatgtag aaacgacttt cgaaggggta
    44461 ttgaagaata tcgagcgtcg ttatcatgat acgaacagtg atttcacgcg tgagcaaatg
    44521 cgtctttata tgacagaatt aacttgtcag acatgtaaag gttatcgatt gaatccccaa
    44581 gctctatctg ttcaaatcaa tggaacgaat atcgggcaaa caaatgagtt gtctattcaa
    44641 aaagcagtag atttcttctc aaacttaacg ttatctgaac aagagaaagt aattgccaaa
    44701 ccgattttaa aagaagtaaa tgacagactt tcattcttgg aaaatgtggg actagactat
    44761 ctaactttga gtcgcgcttc cggaacatta tctgggggag aagcgcagcg aatcagattg
    44821 gctacacaaa ttggatcgaa tctttctgga gttttgtata ttttagatga accatcgatc
    44881 ggattgcatc aacgcgacaa cgatcgactg attggctcat taaagaaaat gcgtgatctt
    44941 ggcaacaccc tcgttgtagt cgaacatgat gaagatacga tgtgggcagc agattatttg
    45001 attgacgtcg gacctggggc cggagaacaa ggcggacaaa tcgtggctgc aggcactcct
    45061 gaagaagtag ctaatgatga aaattctttg acagggaaat atctttcagg gaaaaaatca
    45121 atccctgtac caaaagaacg ccgtaaaggt aatgggaaag caatcaagat cactggtgct
    45181 tcagaaaaca atctgaaaaa tattagtgtt gaatttccat taggagaatt tgtagcagtc
    45241 acaggtgtgt ctggttctgg aaagagtaca ttggtcaata gtatcttaaa gaaatcgtta
    45301 gcgcaaaaat taaataagaa ttctgctaag ccaggtaaat tcaagacaat ttccggctac
    45361 gaaagtatcg aaaagatcat cgatatcgat caaagcccaa tcggccggac gccgagaagt
    45421 aatccagcga cttatacaag tgtatttgat gatatccgtg ggttatttgc tcaaacgaac
    45481 gaggcaaaaa tgcggggtta taagaaaggg cgttttagtt tcaacgtaaa aggcggtcgt
    45541 tgtgaagctt gtcgcgggga tggaattatt aagatcgaaa tgcacttttt gcctgatgtc
    45601 tatgttcctt gtgaagtatg tcatggtaaa cgatataatt ctgaaacatt agaagtgcat
    45661 tacaaaggaa aaagcattgc tgatattttg gaaatgacag tagaggatgc tgtagaattc
    45721 ttcaagcaca ttccaaagat tcatcgcaaa ctgcaaacga ttgttgatgt tggcttaggt
    45781 tatgtgacga tggggcaacc agcaacgaca ttgtccggtg gtgaggcaca acggatgaaa
    45841 cttgccagtg aattgcacaa aatctctaat ggaaagaatt tctatatact agatgaacca
    45901 acgacaggac ttcatagcga tgacatcgcc cgcttgttgc atgtattaca aagattagta
    45961 gatgctggta acacagtttt agtgattgaa cacaatctag atgtaatcaa aacagcagat
    46021 tatatcattg atttaggacc agaaggtgga gaaggtggag gaacgatcct tgcgactgga
    46081 acaccagaag aaatcattaa cgtaaaagaa agttatacag gtcactattt gaaaaaaata
    46141 atggtataaa gaggatagat agctgagata gttaaaaaaa cataaaaaaa tgaatttttg
    46201 cttgttttat taatatcagt gttctagaat cattacatgg tagttctttc tacactgatt
    46261 ggtatagata aaaacttcct ataagttcaa tccgttcaaa aaagaattga gcaatcggag
    46321 gactttcctt tgattgcttt tttctttatg gtatgatatt ccttgtgtag ttggaaaaga
    46381 aaaacaagag attatgagta agtgagagat aatgatgaac agtattgaaa atgaagaatt
    46441 tatccctata ttattaggaa gcgacatgaa cgtttatggt atggcacgtt cttttaatga
    46501 agcatacggc aaaatctgcc aagcatatgc ttcggatcag ttagccccta cccgctacag
    46561 taaaattgtc aatgtagaag ttattccagg atttgataaa gaccctgtat ttatcgagac
    46621 gatgcttcgt ttggcaaaag aaagatattc tgataagagt aaaaaatatc ttttgattgc
    46681 ttgtggtgat ggatatgcag aattgatctc ccaacataaa caagagctgt cagaatattt
    46741 tatctgtcca tatattgatt attctttatt tgaaaggctg atcaataaag taagctttta
    46801 tgaagtttgt gaagaatatg atcttccgta tcctaaaaca ttgatcgtca gagaagaaat
    46861 gttagtaaat ggacatttgg aacaggaatt gccttttgaa ttcccagttg ctttgaaacc
    46921 tgcaaacagt gtggagtatc tttctgtcca atttgaagga agaaaaaaag cttttatttt
    46981 agagactaga gaagagttcg atttaatttt aggccggata tacgaagccg gttataaaag
    47041 tgaaatgatt gtacaagatt ttatcccggg tgatgatagt aatatgcgtg tgctaaatgc
    47101 gtatgtagat gaagatcatc aagttcggat gatgtgcctt ggtcatccac tattagaaga
    47161 tcctacacca gcatcgattg gtaattacgt tgtgatcatg ccagattata acgaaaaaat
    47221 ctatcaaacc attaaggcat ttttggaaaa aatcgaatat acaggctttg ccaatttcga
    47281 tatgaagtat gatcctagag atggagaata taaattattt gaaatcaatt tgagacaagg
    47341 tcgtagcagt ttctttgtga cattgaacgg cttgaatctg gctcgttttg tcacagaaga
    47401 tcgagtgttc aacaaaccct ttgttgaaac gacctatgga acgaatcaat cagataaagc
    47461 aagattatgg atgggtgtgc ccaaaaaaat ctttttagaa tatgctcggg aaaatgaaga
    47521 caaaaaacta gctgagcaaa tgatcaaaga aaatcgttac ggtactactg tattttatga
    47581 aaaagaccga tcaatcaagc gctggttgtt gatgaaatat atgttccata actatattcc
    47641 aagattcaaa aagtattttc atgtgaaaga aggctagttc atggagaatt ttttcagtat
    47701 tttaggcgga atgggcacga tggcgacaga aagttttgtt cgcttgatca accaccgaac
    47761 gaaagctaca aaagatcaag aatatttgaa ttatgtctta ttcaatcatg caacagttcc
    47821 tgatcgtaca gcttatattt tagatcgatc agaagaaaat ccaatgccgt ttctgctgga
    47881 tgatattgag aaacaaaatt tattgcgacc aaacttcatt gtgttgactt gtaacaccgc
    47941 acactacttt tttgaagagt tacaagcggc aacggatatt cctatcttgc atatgccaag
    48001 agaagcagca aatgaacttg tccgtcagca tacgacaggg agagtagcga ttctaggaac
    48061 agaaggaagt atgaaagctg gaatctatga acgcgaagtc aaaaatcttg gtttcgaaac
    48121 agtgattcct gatactgcgc ttcaagaaaa aatcaattac ctgatctatc atgagatcaa
    48181 ggaatcggat tatttgaatc aagagctcta ttacgaaatc ttagaggaag cggtagagcg
    48241 cctaaactgt gaaaaagtca tcctaggttg cacagaatta tctttgatgc atgaatttgc
    48301 tgaagataat cattacccag tgatcgatgc tcaatctatt ttagcggatc gaacgatcga
    48361 acgtgcatta gctgaaagaa gcgaggcatt ggatacagca tcggaaaagt aaaaaaattg
    48421 gatttggggc aaatatccta aaagatattt ggtccaaatc tttttgtaaa agaaaaagaa
    48481 aactggtaat gatacgcccg ggatgatata attttattat gcacaatgtg agaggggttc
    48541 gtaaatatga cagataattt gcaattagtc atcataacag gtatgagtgg tgcagggaaa
    48601 acagtagcta tccaaagttt tgaagatatg ggctattttt gtatcgataa tatgccgcct
    48661 cgattgattc caaaattctg ggaattgatc aaagaatcag gtaaagtcac aaaaatggct
    48721 ttaggagtgg atttgcgttc tcgttccttc tttgaagaaa tccaaaacat gctgatcgaa
    48781 atcgaaaata cgaacttcat cgacacttct atcttatttt tagatgcttc agacgaagaa
    48841 ctagtggctc gctacaaaga aacaagacga gcacatccaa tggcgatgga cggtttgatc
    48901 actgaaggga tacgtaaaga acgggcgata ctagacgact tgaaagccca agcttccgtg
    48961 atcattgata cgacaacatt gacaccacga caattacgtg aaaaaatcaa tgagtcattc
    49021 aaatcaaagg gagaccaagg atttcggatc gaatttgtat catttggttt taaatacggg
    49081 ttgccgatcg attcggatat tgtgatggat gtgcgttttt tgcccaaccc tcattatatc
    49141 ccggaactaa gaccattgac cggaaaagat cagccagtct atgattatgt gatgtccttt
    49201 gatgaaacgc aatcattcta ccataaattt ttggatcttc tggaaactgt attgcctgga
    49261 tacattaaag aaggaaaaag cagcttgaca gttgcaatcg gctgtacagg cggtcaacac
    49321 cgttctgtgg cattgactga acgtttggca aatgctttag ggaaaaacta taaagtcaat
    49381 gtgacacatc gggacaagga taaaaggaaa gagacggtga accggtcgtg aaaatgaaaa
    49441 cctaccggat aagaaaacca aagatcgtag tggtcggcgg tggcactggt ctgccagtta
    49501 tcctgaaaag tttacgaaat caaggagcag atatcacagc agtagtgact gttgcagatg
    49561 atggtggaag cagcggtgca atcagagaat cgatcgctat ggctcctcct ggtgacttga
    49621 gaaatgtctt agtggcgtta tcggatatgc cacaattcta tgaagatatt ttccaatacc
    49681 gtttcaaaaa ggaagatcag tttttagcca atcatacgat cggtaatctg atcattgccg
    49741 cagtttccga gatgcgggga agcacttatg aggcgattca attattagca aaaatgatgc
    49801 atgtagatgg acatgtttat ccttcttcag aaactccttt gaccttgcat gctgttttta
    49861 aagacggaac gactgcagta ggagaatcta aaatcgcgat caaccgtaaa accattgacc
    49921 gtgtgtatgt ctgcaacaca aatgataaaa gtgaagccaa agcggctcga aaagtcgttt
    49981 cagcaattat ggacgccgac atggtcgttt tgggacccgg aagtttattc accagtatcc
    50041 ttccaaattt agtgatacca gaaatcggcg aagcaatgct caagacaact gctgaaaccg
    50101 tctatatctg taatatcatg acccaaaaag gggaaacgga acatttcaca gatgccgatc
    50161 atatacgtgt tttacacaag catttgaatc agccttttat cgatacagta ttggtcaata
    50221 cggaaaaagt gcctgaagat tatatggacc cagagatcta tgatgagtat ctagtacaag
    50281 tcgcacacga cttcaacgga ttgagaaatg aaggatgtcg agtgatttcc accgactttt
    50341 tagaattgaa agatggtggt gtcttccacg atggagacaa agtagtagaa gaattgttcc
    50401 gtttagtttt cggttcaaaa tattaagcaa tttattgttg gagaaaggag gagaatagga
    50461 tgtcatttgc tgcagatgtg aagaaagaac taactggttt ggaagttcat cgagaacatg
    50521 cgaaagcgga attagctgct ttgctgcgaa tgaacggctc tttaggtatt gtgaaccagc
    50581 agtttatttt gaacgtccaa acagaaaatg ctgcaattgc cagacgtatc tattctcttt
    50641 tgaaagatca ttatcaggta cgaagtgagc tactcgttcg taaaaaaatg aaattaaaaa
    50701 agaacaatgt ctatatcgtc cggttgaagc aaggcacaaa agatgtgttg atggatcttg
    50761 atatcatgga tggtatgatg ttcaatggac acgtttcgga cgagattatg ggaaattcgc
    50821 aaaaaatgcg ttcttactta cgtggtgctt ttatggcctc aggttcagtc aataatccgg
    50881 aaacgagtcg ttatcatttg gaaatatttt ctatttatga acagcataac caagacattt
    50941 gcgatatgtt aaattattac ggtctacatg ctcgaacgct tgaacggcgc aatggctata
    51001 tttcgtattt aaaaggagca gaaaaaattg ctgatttctt aacattgatt ggtgcaacca
    51061 actcaatgct ccgatttgaa gatgttcgaa tcgtacggga tatgagaaat tcggtcaatc
    51121 gtctagtcaa ttgtgagaca gcaaatctga acaagacgat tgatgcggct tctaaacaaa
    51181 tcgaaaacat cgaattgatc gaagcaaagg tcggtctaca tgcccttcct gaaaaacttc
    51241 aggaaatcgc agaacttcgg cttcaacatc cagaggtcag cttgaaagaa ttaggggaaa
    51301 tgattccctc aggtgcaata tcaaaatcgg gaattaatca ccgaattcgc aaaataaatg
    51361 aatttgcgga aaaactgcgt gaagaagcag tttaatccaa agaaaaaact gatacatgaa
    51421 gcaggagatg cgaatgtatc agtttttttt aaaagctata cttgcttttc ttccgtcaaa
    51481 ctataattcc aactttactg gagtaacata atgttgaaag gcttctattg ttttttaata
    51541 caatcgtata aaaagcgggg agacaactga atgaagattt tagttttata cataaacttt
    51601 tgcttaatat agaagtcctg atatagatag gatcaagtat ttatctataa ctggagatta
    51661 attaaaaact ggaaggaact gctcgatcaa ccttgagaac tgtttttctt acttctggtc
    51721 aagattctcg tggttggtgg ataaggatgg ttgataagtt cattggatta gctagtcgaa
    51781 aacaaccagg tgtaattaat gtagaagttt taacaagggg atggagtgat ttattcagtg
    51841 ataatcataa gttcataaat agtaattggg atgtatggtt ggtatacgtt atacctgaac
    51901 ttgaactatc aagatttggt ttagaatttg agaatcgatg tttataaagg gagggcaaaa
    51961 aatgtgcttt tttacaactt ttactatcat tatgattgca gtacttttta tttttctaga
    52021 tatagcaaag cgaaatacag catttttatt atatcgggtt ttacttcgag caggtctcat
    52081 aacatttata tcgatcgttg gattttttct attcacagtt attgtattta tttggagaac
    52141 tacagcacca ccattgccag agattacata tggagaattt ccgtttagtc ttgaatatga
    52201 aatagatgag gaaagaattt tgattgaaga tacggtgatt gcagaattta taggaagttt
    52261 tagagggaat gtgacaactc aacctagacg aagttggagt ataaaacttt taaataatag
    52321 tggagagttg aaagcatttc attatgaatt cttaataata gaaagtgaag atgtaacaat
    52381 cactttaagt agtggctctg ctggatattt tatgaatgat ggtggaagca agccacagct
    52441 cacagtccgt gatcgtattg caggttttat gcatttttct cactatgact tagaagaagc
    52501 tcgtaaaata cttgcagaat atgggatcac attaatcaat tgggaatatg cgccaccgat
    52561 aagaaatttt ttcaatgatt attattagtc ttataaaaaa cagaatgtaa aatttatgtg
    52621 cgcgagtatt tagttttcca ttttttgttt gatagatata tagcattgtt gaagcatggg
    52681 atagtggtgc caattttatc aatgaggttg gtcaggcagt taacataatt ggtgatgcta
    52741 taaatccatt taattggaat tggaggaatt aggtgtggaa acagtgagat tatggatatt
    52801 catgaaaaat tttcgttcgg ctttgagata tgttattctg gaatcaaaat caggttatta
    52861 tctgattgac acagatcact atactttttg gggaggctac ctttttccga tttttaattg
    52921 gataatcccc cataaggtta tgaaaattaa cttgcctgca gaagaaataa atagattaat
    52981 actcaacaaa gatttacaaa ggaaaagaga gaaagaacat tctaatgttg cttggatagg
    53041 tccagcattt gcagtagtga cttcttcaat tgtcggtcct atgctagaga gtatttatag
    53101 tgatttttct attggaatac gttatttatt tgttacttta agtatagctg ttcttttgtg
    53161 cataaagggt tatgttagca agaagaataa agatatttta acactaatcg gtgtaaacaa
    53221 tttatctagt actaaaatta tactgtttcc tgaatctata aaagaaggat caaggttgtt
    53281 gataattttt ataatgctta tactgttttc tgttttattt ggggt
//
LOCUS       DANKAZ010000008        51507 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000008 DANKAZ010000000
VERSION     DANKAZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            367..3174
                     /locus_tag="PWY67_000428"
     CDS             367..3174
                     /locus_tag="PWY67_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2056738.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3365..3856
                     /locus_tag="PWY67_000429"
     CDS             3365..3856
                     /locus_tag="PWY67_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2056739.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3959..4948
                     /gene="manX"
                     /locus_tag="PWY67_000430"
     CDS             3959..4948
                     /gene="manX"
                     /locus_tag="PWY67_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL2056740.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4981..5784
                     /locus_tag="PWY67_000431"
     CDS             4981..5784
                     /locus_tag="PWY67_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL2056741.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5805..6719
                     /locus_tag="PWY67_000432"
     CDS             5805..6719
                     /locus_tag="PWY67_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL2056742.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6802..7176
                     /locus_tag="PWY67_000433"
     CDS             6802..7176
                     /locus_tag="PWY67_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL2056743.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7315..7560
                     /locus_tag="PWY67_000434"
     CDS             7315..7560
                     /locus_tag="PWY67_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2056744.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7732..8796
                     /locus_tag="PWY67_000435"
     CDS             7732..8796
                     /locus_tag="PWY67_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2056745.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9042..9467
                     /locus_tag="PWY67_000436"
     CDS             9042..9467
                     /locus_tag="PWY67_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2056746.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9460..9909
                     /gene="nusB"
                     /locus_tag="PWY67_000437"
     CDS             9460..9909
                     /gene="nusB"
                     /locus_tag="PWY67_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL2056747.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10006..10860
                     /locus_tag="PWY67_000438"
     CDS             10006..10860
                     /locus_tag="PWY67_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL2056748.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10861..12198
                     /locus_tag="PWY67_000439"
     CDS             10861..12198
                     /locus_tag="PWY67_000439"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL2056749.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12203..12427
                     /locus_tag="PWY67_000440"
     CDS             12203..12427
                     /locus_tag="PWY67_000440"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL2056750.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12429..13319
                     /locus_tag="PWY67_000441"
     CDS             12429..13319
                     /locus_tag="PWY67_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2056751.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13328..14146
                     /locus_tag="PWY67_000442"
     CDS             13328..14146
                     /locus_tag="PWY67_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL2056752.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14253..14702
                     /locus_tag="PWY67_000443"
     CDS             14253..14702
                     /locus_tag="PWY67_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2056753.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14720..16402
                     /gene="recN"
                     /locus_tag="PWY67_000444"
     CDS             14720..16402
                     /gene="recN"
                     /locus_tag="PWY67_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL2056754.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16457..16573)
                     /locus_tag="PWY67_000445"
     CDS             complement(16457..16573)
                     /locus_tag="PWY67_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL2056755.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16646..17602)
                     /locus_tag="PWY67_000446"
     CDS             complement(16646..17602)
                     /locus_tag="PWY67_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2056756.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17773..18141
                     /locus_tag="PWY67_000447"
     CDS             17773..18141
                     /locus_tag="PWY67_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL2056757.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18311..18742
                     /gene="mraZ"
                     /locus_tag="PWY67_000448"
     CDS             18311..18742
                     /gene="mraZ"
                     /locus_tag="PWY67_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL2056758.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18763..19722
                     /gene="rsmH"
                     /locus_tag="PWY67_000449"
     CDS             18763..19722
                     /gene="rsmH"
                     /locus_tag="PWY67_000449"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL2056759.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19727..20128
                     /gene="ftsL"
                     /locus_tag="PWY67_000450"
     CDS             19727..20128
                     /gene="ftsL"
                     /locus_tag="PWY67_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL2056760.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20129..22321
                     /locus_tag="PWY67_000451"
     CDS             20129..22321
                     /locus_tag="PWY67_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2056761.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22347..23309
                     /locus_tag="PWY67_000452"
     CDS             22347..23309
                     /locus_tag="PWY67_000452"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL2056762.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23323..24684
                     /locus_tag="PWY67_000453"
     CDS             23323..24684
                     /locus_tag="PWY67_000453"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL2056763.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24710..25798
                     /gene="murG"
                     /locus_tag="PWY67_000454"
     CDS             24710..25798
                     /gene="murG"
                     /locus_tag="PWY67_000454"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL2056764.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25813..27033
                     /locus_tag="PWY67_000455"
     CDS             25813..27033
                     /locus_tag="PWY67_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL2056765.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27189..28514
                     /gene="ftsA"
                     /locus_tag="PWY67_000456"
     CDS             27189..28514
                     /gene="ftsA"
                     /locus_tag="PWY67_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL2056766.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28537..29778
                     /gene="ftsZ"
                     /locus_tag="PWY67_000457"
     CDS             28537..29778
                     /gene="ftsZ"
                     /locus_tag="PWY67_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL2056767.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29795..30472
                     /locus_tag="PWY67_000458"
     CDS             29795..30472
                     /locus_tag="PWY67_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL2056768.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30486..31085
                     /locus_tag="PWY67_000459"
     CDS             30486..31085
                     /locus_tag="PWY67_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL2056769.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31100..31387
                     /locus_tag="PWY67_000460"
     CDS             31100..31387
                     /locus_tag="PWY67_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL2056770.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31436..32218
                     /locus_tag="PWY67_000461"
     CDS             31436..32218
                     /locus_tag="PWY67_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL2056771.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32239..32946
                     /locus_tag="PWY67_000462"
     CDS             32239..32946
                     /locus_tag="PWY67_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL2056772.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33208..36006
                     /gene="ileS"
                     /locus_tag="PWY67_000463"
     CDS             33208..36006
                     /gene="ileS"
                     /locus_tag="PWY67_000463"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL2056773.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36055..37581)
                     /locus_tag="PWY67_000464"
     CDS             complement(36055..37581)
                     /locus_tag="PWY67_000464"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL2056774.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37596..38243)
                     /locus_tag="PWY67_000465"
     CDS             complement(37596..38243)
                     /locus_tag="PWY67_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL2056775.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38427..38756)
                     /locus_tag="PWY67_000466"
     CDS             complement(38427..38756)
                     /locus_tag="PWY67_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL2056776.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38933..39661
                     /gene="sfsA"
                     /locus_tag="PWY67_000467"
     CDS             38933..39661
                     /gene="sfsA"
                     /locus_tag="PWY67_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL2056777.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39677..40690
                     /locus_tag="PWY67_000468"
     CDS             39677..40690
                     /locus_tag="PWY67_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2056778.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40690..41967
                     /locus_tag="PWY67_000469"
     CDS             40690..41967
                     /locus_tag="PWY67_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2056779.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42030..44732
                     /locus_tag="PWY67_000470"
     CDS             42030..44732
                     /locus_tag="PWY67_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2056780.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44884..45201
                     /locus_tag="PWY67_000471"
     CDS             44884..45201
                     /locus_tag="PWY67_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2056781.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45231..45551
                     /locus_tag="PWY67_000472"
     CDS             45231..45551
                     /locus_tag="PWY67_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2056782.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45659..47119
                     /locus_tag="PWY67_000473"
     CDS             45659..47119
                     /locus_tag="PWY67_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2056783.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47187..47408
                     /locus_tag="PWY67_000474"
     CDS             47187..47408
                     /locus_tag="PWY67_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056784.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47439..47621
                     /locus_tag="PWY67_000475"
     CDS             47439..47621
                     /locus_tag="PWY67_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL2056785.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47621..48034
                     /locus_tag="PWY67_000476"
     CDS             47621..48034
                     /locus_tag="PWY67_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL2056786.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48157..49338
                     /locus_tag="PWY67_000477"
     CDS             48157..49338
                     /locus_tag="PWY67_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2056787.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(49409..49573)
                     /locus_tag="PWY67_000478"
     CDS             complement(49409..49573)
                     /locus_tag="PWY67_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056788.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            49727..49800
                     /locus_tag="PWY67_000479"
     tRNA            49727..49800
                     /locus_tag="PWY67_000479"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49761..49763,aa:Arg,seq:cct)
     gene            complement(49837..50037)
                     /locus_tag="PWY67_000480"
     CDS             complement(49837..50037)
                     /locus_tag="PWY67_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2056789.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(50342..>51507)
                     /locus_tag="PWY67_000481"
     CDS             complement(50342..>51507)
                     /locus_tag="PWY67_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL2056790.1"
                     /translation="EKGHTVVLQISVEQAPLARLITQEAYKLGAAEVIVQWTDDQIQR
                     EFLLHAATDRIENVPQSKIDQADEWLEKGASRISVVSADPDAFAGVDSHRVASYQSAN
                     GKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPELPEEKQVEALWDQIFKTTRVYEE
                     DPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGTDIIIGLPKNHLWEGAGSYNARGE
                     KFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGTIISGMKFTFKDGKVVDFSAEQGE
                     DVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIFFNTLFDENASNHLALGSAYAFSV
                     KGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDIDGIREDGSTVPIFRNGDWA"
BASE COUNT        17043 a         8779 c        11091 g        14594 t
ORIGIN      
        1 aagttgtagt ggaggctacg aagtttactt tgtcttttta cacttaatat ttttttgcca
       61 atttggaatc aggatgtttt gataactatc gttttcaatt gatggaagct atattgtttg
      121 ctatcagtga aataattata tggtaaacag tgttcttttt cttgtttcca ttataaaaat
      181 cgtatcggga tttttcgttc ttttaaattg aacatttcta tctatccgcg tgttatcaac
      241 aaaacaaaat caggtaaata gttgacgaaa cagaaataaa tacatagtat tatgttttga
      301 agcctaagta caatagtcta tagaaaagga gcgataccat tggcaaaacg cattgaccga
      361 atttatatgt atgtcaaaga aaaaaccgct catttaacaa aggcagaata tgatcaagga
      421 gtaacaacac aggaaattgc tgaagtgtta gggattcagc gaacaaatag cagtaaagat
      481 ctgaatcagc tagtgcgcga aggaaaactg ataaagacag acggacgacc agtacgttac
      541 atatatcaaa cactcgttac gcacgtaaag ccagttacaa aacatgtagt cagttacaaa
      601 gaaacgccgg tctctgaaga aaaaaataca ctccaaatag atactaagga tatctttgca
      661 aaaatcattg gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt
      721 ttgtatccgc caagaggatt aaattgtttg attacaggac ctaccggatc aggaaagact
      781 tattttgcac acgcgatgta tcattttgca aaagcgcatc atgtaataga tgaggaaacg
      841 gaactgattg ttttcaattg tgctgattat gccaataacc ctgagctttt gatgagtcat
      901 ttatttgggt acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat ggggattatc
      961 gatcaagcgg atggcggtat gctgtttttg gatgagattc accggttgcc gcctgaaggt
     1021 caagaaatga tcttttactt tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg
     1081 aaatcccatg aagcaaatgt acggatcgta ggtgcgacca cagaagaccc aggttcttct
     1141 ctattagaaa cttttgttcg gcgtatccca atcaatatca aacttccggc ttttgaaaaa
     1201 cgtcctgcaa atgagaaagt cgatttagta aaaatcatga ttgcccacga agcaaaccgc
     1261 acgcagcgaa agatttcttt gacagaagat gtagcaaaag cattgatcgg cagtgtgacc
     1321 tatggaaata taggacaatt gaagtcaaat atccagttag tatgtgcacg aggatttcta
     1381 aatcatatgc aatcacccga aatctcgatc acaatcgatg atttgacaga agggattcgt
     1441 agcggattga ttcaattagc aagcaaccgc agtgcaatgg cagagctttc taaaatattg
     1501 gagccaaaga taactgtagc accaaatgat acgatcatga aaatccaatc ggactcctat
     1561 gagttacctt ataatctata tgatatcatc ggagataaag cagctttact gaagtcggat
     1621 ggtttggatc aagaagcaat caatcatttc atttctacag atatcaacat ccatctgaag
     1681 tctttctata aagaccatgg tttttctttt aatgcagaca gcaaattagc agaatttgta
     1741 gatccgaaaa tcatagaagt gaccaatcag atttatgtaa tggtcaaaag atctttacca
     1801 tatgagtttc agcaaaactt tatttatgct atgagcttgc atatcagttc ttttttgaaa
     1861 aaaatcaact taggagaaga acggcatacg aatgataaca tccgtgagat ggcttttgat
     1921 tttccagatg aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa
     1981 gtggagattc cagaaagtga agactattac ttgaccgttc tactcgtctc gttacgggca
     2041 aatcaagcaa gcggcagaat cggtgtagtc gttgcagctc acggaaacag cacagcaagc
     2101 agtatggtcc aagttgtcca acagttgtta gatgcagaga acgtacgagc agtagacatg
     2161 cctttggata tggatccgaa aacagcattg gaccgaattg aacgcaatgt acaagaagta
     2221 gatgaaggaa gcggggtcat actcttagta gatatggggt cccttgcgtc gtttaattcg
     2281 caaattcaaa gagacactgg aattccagta cgtactgttg atatggtaac aacttctcta
     2341 gttttggaaa ctgtacgaaa ggtctcagta ttagggacag atctagatat gttgcatgaa
     2401 tcattgaaaa attttcgagg ttacgcagaa atatcttctg tagaatctgt tcaggaagca
     2461 aaacctattc ataagaaaaa agctgttttg gctatctgcg catctggcga aggtacagca
     2521 caaagaatca aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa
     2581 gttctagctt tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca aaaagaatat
     2641 cagatcatag ctactactgg gattactgat ttgaatatcc aagccccatt tattccttta
     2701 gaacgtttta ttgatcaaaa tatcgaagtc attttggatc agttgctgat ggaatcagaa
     2761 ttggaggaaa ctgaattcat ctccctagat gaagagtcag ctaaaaatac gtgtgttgag
     2821 tttatctctg ataacttcat ctttattaat ggtagtaagt taatcgatcc aatgtggcaa
     2881 ttttctacac aaatctctca gacgacggga atcggcgatg aagaatatgg attcaaaatc
     2941 aacctagtga tgcacacagc aggaatgatc gaacgtatta ttcggaatga accattgact
     3001 gtggaagaaa acgaattgac aaatacaacg aatgatcctc tttatagcca attggcagcc
     3061 tcagttgttc tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga aatgtattat
     3121 ctgctacgtc tagtccataa tcagttagat aaaaaagaat acactgtgcc atgaatacac
     3181 aaaaacgata cacttttata gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt
     3241 caattagtgt atcgtaaaaa agttggcacg ctttttgctt atagtagtgt gtgctaggag
     3301 gtggttgttc acacagaaat gataggggtt tctgtgaagt agtgaggaag aaggagtttt
     3361 gcagatggat attcgattgg cacgtataga tgatcgttta atccacgggc aagtcgcaac
     3421 agtttggtcg aagcaaacag gtgttgaacg tatcattgtg atcagcgatg tagtcgctca
     3481 agataaattg agaaagtttc tactaaaaga agctgcaccg ccggggatca cagcaaatgt
     3541 aataaccgtg cagaaaatga ttcaaatcta tcatagtgga ttattatcac gagcaaaagt
     3601 gatgctttta ttcaccaatc cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt
     3661 gaagtcgttg aatatcggcg gtatgagttt tactgaaggc aaatcaatga ttaccaattt
     3721 tgtttcagta aatcaacaag atattgattc ctttcgttac ttgaacagtc aagaagtgga
     3781 gttagagatt cgaaaagtac ctgctgacag gaaaatcctt ttgatggaac tactagcgaa
     3841 aatcaaaaag gtgtagcaag aatggttgtt ggaaaaggtc aaataattct gttgtaatgg
     3901 ttagcaatta ttaatatttt tgaatttgtt acaacaaaag taaataggag gtataaatat
     3961 ggtagggatt atcctagcaa gtcatggaca atttgctgaa ggaatcttac aatccggttc
     4021 aatgatcttt ggcgaacaag aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc
     4081 agatgacttg agagccaaat tggaagaagc ggttgcttct tttgataatc aagacgaagt
     4141 gttattctta gttgatttat ggggcggaac accgttcaat caatcaaaca cattattcga
     4201 agagcacaaa gataaatggg caatcgtcag cggtttgaat ttaccaatgt tgattgaagc
     4261 ctacgcatca cgcttttcaa tggaatctgc tcatgaaatt gcagcacaca tcattgaaac
     4321 agcaaaagac ggtgtaaaag taaaaccgga agaattagaa ccggcagaag caccgaaagc
     4381 agcagttgaa gacgcacaac ctaaaggagc acttccagaa ggaactgtgg tcggagacgg
     4441 aaaaatcaaa tatgtattag cacgtgttga ctcacgtctg ttacatggac aagttgcgac
     4501 agcttggaca aaagcagtgc aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa
     4561 ggatgatctg cgtaagagac taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt
     4621 tattcctatt agcaaaatga tcgaagtagc aaaagaccct cgttttggaa ataccaaagc
     4681 attgctttta tttgaaaatc ctgaagacgt attgacagct gttgaaggtg gcgtagacat
     4741 caaagaatta aatgttgggt caatggcgca ttcggtcggt aaagtcgttg taagtaaagt
     4801 attgtctatg ggacaagaag acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa
     4861 attcgatgta cgtaaagtac ctaatgattc acgcgataac atggatgata ttttgaaaaa
     4921 agcaaaagct gaattagcaa aagcgtaata aataatatta aaatttatag gaggcttatc
     4981 atgtctatta tttcaataat tttagtcgta ttagttgcct ttctagctgg tatggaaggt
     5041 attcttgatg aatttcaatt tcatcaacca ctagtggcat gtacattgat cggtttagtt
     5101 accggtaacc tcgaagctgg tatcgtactt ggcggtacgc ttcagatgat cgctttaggt
     5161 tgggcaaata tcggtgccgc tgtagcacca gatgctgcat tagcatcagt agcatcagca
     5221 attattttag ttttaggtgg acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc
     5281 gttcctttgg ctgttgcagg gttgttctta acaatgatcg tacgtacaat cgctgttcca
     5341 atcgttcatt tgatggatgc ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg
     5401 cacatcattg ctgtatgttt acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta
     5461 tttatcccag cacaaactgt gcaatcattc cttgaatcaa tgccagcatg gttaacagat
     5521 ggtatggcta tcggtggggg aatggtcgta gctgtaggtt acgctttggt aatcaacatg
     5581 atggctacaa aagaagtatg gccattcttc gttattggtt tcgttgtagc tgcaatttct
     5641 caattaacat tgattgctct aggtgcttta ggtgttgcat tagctcttat ttacttgaac
     5701 ctttctaaaa tgggtggttc atcaaataat ggaggcggaa gcaactctgg tgacccttta
     5761 ggcgacattt tgaatgacta ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa
     5821 tcaaattatc aaaaaaagat cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt
     5881 catggaacta cgaacgtatg caaaacggtg gctgggcatt ttctatgatt ccagcaatca
     5941 aaaaattata caaaacaaaa gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct
     6001 ttaatacaca cccatacatt gcttctccaa ttttaggggt tacattggct ctggaagaag
     6061 aacgtgcgaa tggcgcgcct gttgatgatg tagcgattca aggggttaaa gtaggtatga
     6121 tgggtccttt agctggtgtt ggtgacccag tgttctggtt tacagtacgt ccaatgctag
     6181 gtgcactagg tgcttcatta gctatgggag gaaatatcct tggaccaatc atcttcttct
     6241 taggttggaa cattattcgt tgggcattca tgtggtatac acaagaattt ggctacaaag
     6301 ctggttctaa aattactgat gacctttctg gcggattgtt acaagacgta acaaaaggtg
     6361 cttcaatttt agggatgttc gtattagctg cccttgtaca aagatgggta tcaatcaaat
     6421 tcttaccagt tgtatcaact gtaaaattag ataaaggtgc ttacatcgaa tgggataaat
     6481 tgccttcagg tggtgaagga atcaagagtg catttgaaca agttaataat ggcttagccc
     6541 tttctcctga aaaggtaaca acattacaag acaacttaga ccaattgatt cctggtttag
     6601 ctgcattagg attaacattg ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg
     6661 ttatcatcct tggtttattc gtagtcggtg ttgttggaca cgtcatcgga cttttataat
     6721 ccataaaaat atagctcggg cgttgtcccg agcttttttt atatagtata ctgttcataa
     6781 acaaatatct ggagggagaa aatggttcaa tcaatcaata caaaagtaga cttggtaatt
     6841 gatgctactt cgtttacggg tctgacagat tacgggcaaa tcatgatagg tgataatggt
     6901 tttgagtttt accatgcgcg agatagacgc aaatatatcc aaattccatg ggaagaagtg
     6961 gattatgtac tagcatcagt catgctaaag ggaaaatgga ttcctcgata tgccattcaa
     7021 acgaaaaaaa atggtacata cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg
     7081 atcagagaat atgtagatga agatagaatg gtacaatcat tgagtttctt tgatgtagtg
     7141 aaacgtggta ttcgtagtat gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg
     7201 aggttttctt tattttttta ctttgcagaa gaaaatatga aatagcaaaa ccaattattt
     7261 gtccccttta aatcccatat gctaaaattg attagcaaag aaggaggtta tattatgagt
     7321 tggttatgga cattgattgt tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt
     7381 aaaggaaatt cgatgggctg tgtaacgaat atcattgctg gactagtcgg ttcaagtatt
     7441 ggacaagcga ttttcggcga atggggacca aaagctgcag gtatggcact tattccatct
     7501 attttaggcg cagtgatttt ggtagcagtt gtctcatttt tctttggaaa acgatcataa
     7561 gtttttacaa aatagtaaac taaaaggagt tgcagcgaga ctgtgactcc tttttggctt
     7621 tatttattta aaataaaaag aagttttccg gaaattttta tttaactgtc gtgtacgaag
     7681 aaaagtttgc tataatggaa aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta
     7741 cgtgtagaaa aattgcgcaa aaaaatgcaa gaagaaaatt tggattcatt tttggttacc
     7801 agtccgtaca atcttcgtta cttaacgaac tttacaggta cgacaggatt agctgttatt
     7861 actttggaaa aagcattttt tattactgat tttcgttata ccgagcaggc ggcagcgcag
     7921 gctcaagggt ttgaaattat taaaaatgtc ggacctattt ttgatgaggt agcaaactta
     7981 gtacgcaaag aagaactgca ctcattagct tttgaggaaa caacagtctc atttttagaa
     8041 tattcggttt tagaagaaat catcgatgcg gaattagtac ctgtttcaca gatgatcgaa
     8101 gaactcagag aagtgaaaga tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt
     8161 gcggatatgg catatgatca catcctgaaa atgatccaac cgggcatgac tgagattgaa
     8221 gtcgcaaatc aactagactt ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat
     8281 acgatcgttg ctagcggatt gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc
     8341 atcgaacaag gtgacttgat cacattggac tttggttgct attacgaagg gtacgtttcc
     8401 gatatgacac gtacattcgc tattggtgat cctggggaaa aattaaaaga aatttatcaa
     8461 atagtcttag atgctcaatt agcagttatt gatgctgcaa aaccaggaat gactggtgtc
     8521 caattagatg cagttgctag agattatatt tcaaaacatg gttacgggga agcatttgga
     8581 cattctacag gacatgggat cggtttagag atccacgaag gaccaaacgt ttcttctcgt
     8641 gcagaaaagc aatttgtacc aggaaatgtg atcactgatg aaccagggat ctacttacca
     8701 ggaatcggtg gagtacgtat tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg
     8761 ttgactcgtt caccaaaaga attgatcatc ttatagtttc atcaatagaa gaagtaagcg
     8821 taaaaatcag tgttcacaga ctttgttata gaaaatcact cgatttaatg atacactata
     8881 gaagaacttt taagctgtaa gagataataa agttgggtgt tttctccaat tcatagcagt
     8941 ttgcagctga tcggcggcag ttttcttacc acgtgatcac tatgccgcat catagagaat
     9001 tcggatgtat ctcgtgctta ggcaaaggga ggaataaata aatggcagat gaaaaaaatt
     9061 tgattttaaa cgcaaaccaa gatttaggag agatcgtgat cgctcctgaa gtcatcgaag
     9121 taattatcgg gattgcagct tctaaagtag aaggcgtata cggaatgcgc ggaacttttg
     9181 ccagcaatgt cactgaatta ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa
     9241 acgaggaaga tggcttgaaa gtagatcttt actgttatct agaatatggt gtttcagttc
     9301 ctaaagtagc aatggaaatg caggaacggg tcagccagca agtccttttc atgacggata
     9361 tcgatttatc tgaagtcaat atccatgtag tagctgttgt tcctgaaaaa ttagaacaac
     9421 caacaatcga agaacttttt gaagacgaag aggaagagaa tgaataaaga attatcaaga
     9481 cacgagattc gggaaatggc attgcaggct ttatttccat tagacttcaa tgcagaccta
     9541 acaaaagaag atgcgatttt caacgcaatc gaattagacc accgcgacat gatcaacgaa
     9601 gatgaatcag agtttgttcc tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa
     9661 aaagatgaat tggataaagt gattgaaaaa catttaaaaa acaattggca catcaatcgg
     9721 atctcaaaaa tggatttggt gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca
     9781 gatgtaccag cggctgttgc tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat
     9841 gaccgttcac gtaaattcgt caacggtgtt ttatccaatg tattgaaaga attagaagct
     9901 ggggcgtaag tcctagcttt tttctatgaa tcatcataaa cctgttataa atcgggaaaa
     9961 ttctatggta gaatgaaaac acaaaaaata tctgggagtg gttagatggc agaattgatt
    10021 aacggaaaag aattagctga aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg
    10081 aaagataatg gtatccatcc cggattagtc gttttattgg taggggaaaa tccagccagc
    10141 caaatctatg taagaaataa agaacgtgta gcaaaagaaa tcggaatcca ttcattggta
    10201 gagcgttatc ctgagacgat ttctgaagac gaactacttg cagaaattga aaaatacaat
    10261 caagatccta gttttcatgg tatattggtc caattaccat tgccaaagca tatagatgaa
    10321 gaaaagatcc tgttagctat ccgccctgaa aaagacgtag acggttttca tccgatgaat
    10381 ttaggcagac tccttgcggg taaaccggac aagattcctt gtactccata cggcatcatg
    10441 aaaatgtttg aagcttattc tatcgatccg gcgggtaaac gagcagtagt tatcggacga
    10501 agcaatatcg tcggtaaacc gatggctcag ctactcttga tggcagatgc aactgttact
    10561 gttgctcatt ctaaaaccga aaatctggct gaactggcac aagaagcaga tattttagtc
    10621 gtagcaatag gacgtggaca ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg
    10681 atagatgtag ggatgaatcg tgatgctaac ggtaagctga tcggtgatgt gaaatttgat
    10741 gaagtggagc ctattgcagg ctggatcact cctgtgccaa agggtgtagg gccaatgact
    10801 atcacgatgc tgatgtatca aaccgtaaaa tctgccgaag cttttggagc aggtgaatag
    10861 atggcacaag aatatttaac tgtcactaca ttaacgaagt atttgaaaag aaaatttgat
    10921 gccgatcctt atttagaacg cgtatattta acaggcgaaa tatcgaattt ccgtttacgt
    10981 gccaatgccc atcaatattt cagcttaaaa gatgaccatg caaaaatatc tgctattatg
    11041 ttcaaaggag cattccaaaa attacgattc cagccaaaag aaggaatgaa agtccttgtc
    11101 gttgggcgta tttcattata tgaagcggga ggttcttatc aaatctatgt agagcacatg
    11161 gagcctgatg gaataggtgc tttatatcaa gaattggaag aacgaaaaga aaagctgatg
    11221 aaggaaggcc tttttcaagg gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg
    11281 gtccttacca gtccaagcgg agcggttatc cgcgatatca tcacgacggt gaaaagaaga
    11341 tatccaattg tccaattggt tttgttccca acagttgtac aaggaaacca agcagcggac
    11401 aatgttgtca ggaatatcca gagagtagaa gagatgggca atttcgatac aatgatcatc
    11461 ggtcgtgggg gtggttcgat tgaggattta tggccgttta atgaagagcg ggttgctcgg
    11521 gcgatttatc aagctaatac gccggtgatt tcatctgtag gacatgaaac agataccacg
    11581 atcgctgatt tagtagcaga tgttcgggct gccactccaa cagcagctgc agaattagct
    11641 gttcctgttt tgtcagaaga gttgatgcgc attcaagaac ggcaagctag attgcagcag
    11701 gcgtttgtac gccagatcca gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat
    11761 gtatttcggc agcctgaaag attgtatgaa ggtcagacca tcaaattaga ccagttgaaa
    11821 cagcgattat atcaagctac acagcaaatt tatcatgcga aagaaaaaca aacgatcgca
    11881 ttaagccacc aattggaaca agtggctccg ctttatcgag ttaaagcagc tcgccaagaa
    11941 actgcttatc tagaaaaacg cttagaagag aaaatccaac aatatatgaa acaacagcag
    12001 cagcgttttg ctcaagctgt tcaatctctt gatttgttga gtccattgaa aatcatggga
    12061 agaggctaca gctatacgac gttaaatgat caagtagtaa aaagtgtcac cgagattcat
    12121 caaggggatt tgctgacgat ccattatcaa gatggaatcg ttaaaggaca agtacaagag
    12181 atagacaaag gagaataaaa atatggaaaa tccaactttt gaagagtcac tgcaagaatt
    12241 agaaaaaatt gtcatgcagt tagaacaagg agacgttcca ttagaatcag ctctagattc
    12301 gtttaaacga ggaatggaat taagcaaaca ttgccaagat acattagcta aagcagaaga
    12361 gaccttgacg aaaatgatga cagaatcaaa tgaagaagtt gattttgacg gaaacgagga
    12421 aagctgagat gagattaact gacttttcag ctattcatct acctgctgta gaaaaagaaa
    12481 tcttgtcttt tttagatgag catacaacgg atagaggact ctatgatgcg atgagttatt
    12541 cagtgaaagc aggcgggaaa agaatacgtc ccttattact tttaacggcc gttgcttcct
    12601 ttgatgagcc tattgatgtt cccgtctatc aagtagcagc tgctttagaa atggtgcata
    12661 cgtattcctt gatccacgat gatttgcctg cgatggacaa tgatgatctt cgtagaggaa
    12721 aacctaccaa tcataaagta ttcggcgaag cattagctat attggccgga gatggtttgc
    12781 ttacaggagc attccagcta atcagtatgg cacacttagg taattctcct aagcttctgc
    12841 tgcttcagca attagctgtt tgtgcgggat cacaaggtat ggttgctggg caagctgcag
    12901 atatcgaggg cgaatcgaaa aagctttctt tagaagaatt agcatttatt catgaaagaa
    12961 agacagggca tttgattcgt tatgccttgc tggctggtgg catattagcg aagcaacctg
    13021 aagaaatctt attgctactt caacgattag ccgaacattt aggtttagct tttcagataa
    13081 gagatgatct actcgatgtg attggtacaa cgaaaacatt agggaaaaca gctggtaaag
    13141 atgaacgtat ggaaaaaaat acttacccgc gtttattagg cctagaaaag acacgtgagg
    13201 cgttggagat tgagctgtta tccgccaaca agatcataga caaattagaa gaggacgttt
    13261 tttcttttga tggcgggctg atgcgccaga tgatcaagca atttgatgtg gagaaatgag
    13321 ggaaagaatg gaaaaagaac gcgtagatgt attagcggtc aaacaaggat tatttgaaac
    13381 aagagaacaa gccaaacgtt cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg
    13441 gtttgataaa ccgggagaaa aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa
    13501 acttccttat gtttctcgag ggggattaaa attagaaaaa gcactcaaag catttgatct
    13561 atctgttgaa ggaaaaacat tgttagatat tggtgcatcc actggcggat ttactgatgc
    13621 agctttgcaa aatggcgcaa aaatgagtta tgctttagac gttggctaca atcagctagc
    13681 ttggaaaata agacaagatc ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc
    13741 aaaacccgaa gactttacct taggaattcc agaagttgct gttatcgatg tttcttttat
    13801 ttcactaaaa ctgatgctgc ctccattaca tgctatttta aaagaagagg gagaagtgat
    13861 tgctttaatc aaaccccaat tcgaagcagg cagagaagct gtagggaaaa acggaattgt
    13921 tcgtgaccct cagacccatc aaaaagtttt agaagacatt ttgtcatttg cggcacaagg
    13981 tggatatgat gtgctggaat taagttattc tccgatcacg gggggagaag gaaatatcga
    14041 gttcttggct cacttgagga aagtacccga aagcggaaca atcaattcag ctattaatat
    14101 ggcagaagtg gtttcaaatg cacacgaaca gtttgaccat aaataaattg taactgtgaa
    14161 aatcaagtga gaagtatgcg gattcttctt tattatacca aggaaataag ctatcattga
    14221 ttaagaagaa tatgctaagg agcgaagacg taatgagaaa aaaagatcgg catcgtttga
    14281 ttactcgatt gttgagtgag caagatatcc gaaagcagga agaatttgtt gaaatcctga
    14341 aaagcaaagg aatatctgtt actcaagcta cgatttccag agatataaag gaactgaaac
    14401 tgatcaaggt accggcttca gatggcggct accgttatag cataccagct gaaacaaacg
    14461 aagatgtaaa tactaaattg gaaaaactat taaaagacgc tttcgtggca gttgaccaaa
    14521 tggaaaaatt tgttattttg aaaacattgc cgggaaatgc ttcggcagca gcaaatttga
    14581 ttgataaacg ttacaagaaa gaattatttt cgattatcaa tgatgacgat aatgttttga
    14641 tgattacacg aactgaagac caggcagttt tattgaagaa agacttcctg cattaccttt
    14701 aagttgagga gcgaatgtga tgctacaaga aatcagtatt actaactttg cgattattcc
    14761 ggaattgcgg ctttcttttc acgaaggaat gaccgcgctg accggagaga ctggtgcggg
    14821 taaatccatt attatcgatg ctttaggtct gttagcagga ggaagaggtt caagcgatta
    14881 tatacgtcaa ggagcagaaa aatgtgtgct tgaagggttg tttgaactac caaaacaaga
    14941 aggattctct gaactgatgg ttgaattagg gatcgaaaca gatgaggaca atctgattgt
    15001 tcgacgggat atgtccttaa ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac
    15061 attagctaat ctgagaagaa tcggcagtta tttagtagat atccaaggac aaaacgaaca
    15121 tcaggaactg ctccagcctg aatcacatct tgctttattg gatcgttttg gagatgtagc
    15181 tttccaacag aagaaaaaga gttatcagca agaatatatc tcttatcgtg agctggaaaa
    15241 aagggtacga aaagtgcagc aaaatgaaaa atcctatgtt cagagaatag atatgctcca
    15301 tttccagcaa gaagaaatag ctgctgctga tttgcaagtc ggagaagaag agaaattaaa
    15361 agaagaaaga gaaaaactta gcaattatca aaaaattgct gatggattag ctgcaggtta
    15421 cggagcactg acgaataatg aacaaaacag tgtagatggt gttggcttgg ccgtttcaga
    15481 actgcaaggg atcgcccatc tagatgtgga gtatgaagca atttatgaaa atatccaaag
    15541 tgcttactat ttgctgcaag atgcaatcgg cgatatgagt cgtcagattg atttattgga
    15601 gttagatgag agtcgtttag aagaagttac gcaacgttta gaattaatcc gacagttgaa
    15661 acgtaaatac ggcgaatcca ttgagtcaat attggcttac tatgacgaaa tcaccgaaga
    15721 gctagcttct tctgattttt cagaaggtca attggacaaa atgaaagagg aactagagca
    15781 aaaagagctt ctacttcagc aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc
    15841 aaaagaattg gaaaaatcca ttctgcatga attgaaaagt ctgtatatgg aaaacactga
    15901 gtttgaggta cgtttcttaa aagaagaaaa tagacaattg aatcatgatg gatttgacca
    15961 aatcgaattt tacattacga ccaatccagg agagccactg aagcctttgg tgaaagtcgc
    16021 ttctggtggc gaactgtctc ggatgttgct tgctttgaaa actatttttt cttctgagca
    16081 aggtgtgaca agtattattt ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca
    16141 ggccatcgca gataaaattt ctcaaatatc gaaatactct caagttttgt gcatcactca
    16201 tctgcctcaa gttgctgcag tcgccgacta tcaatattat attgtgaaag cagtgattga
    16261 tggacgtacg caaacttctg tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc
    16321 acgtatgctg gctggaagtg agattacaaa actcacactc gaacatgcaa aagaactatt
    16381 gaaactcgca aaaaggacct gagccacctc gcagtggttc aggtcctttt cagcagctga
    16441 tagctatatt ttatttttac atgacactca ttacagcagt caatacaaca gatccaatcg
    16501 ttaaaatcag cattgtccaa atgacaaatt ttgttacttt agcaaaagta cttgtttgtt
    16561 ttttttcacg cattgttttc aacctcgtca ttaattagtt gattcaagtg tacaatcttt
    16621 tttccatttc cacaagaggg cttttttata aaaacttccc tttttttaaa taataggtag
    16681 ttacgataca aataaataat gttaatagaa ctagccatat aaaggcatca tccctttcag
    16741 caataggtaa cttaatgttc attccataga tactgccgac gattgtcggt atagttaaaa
    16801 taatcgttaa agaagttaaa attttcataa tattattcag attattcgat acgattgctg
    16861 aaaaagtctg attcatttgt tctactagtt ttaattgaat gcgagaagta gtcattgcct
    16921 gctctgtttc caccaataca tcacgtaaat gatttttcaa agcatgattt tcttggaaaa
    16981 gtttattttg cgctagttct ttcaatgcat ctaaattagc agaagtcgct tctctgaaat
    17041 aaacgaggct tttttggata tccatgattt gataaagttg tttgttttca gtggatactt
    17101 gaagtttggc ttccaaagta tcgcattgag attgaagcgt agctaaataa ctgttgtaag
    17161 agagtgtgac ctgccaaatg aattgaaaga agatattgag attaggcgaa atatcacttt
    17221 ttggaaaggt ctggctaaag actttcttta aaaacagtgg ttcatgattc gtaattgtga
    17281 taagcttctt tttgggcgtc aaaataatag agagcgggta tgtatccact tgaaaataac
    17341 cgctaggact tgttttagca aaaggatatt gtaacaataa taacgctggc tttgtcaact
    17401 ttgattgatt gaattcttct gtacgggaat tttctgcatc gtccaatatg gaagtgatgt
    17461 aatctgacgg aatttcaaat tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt
    17521 ctacacaata ccagatcgtt tggtcagttt cctttccggt attacgcagt ttctcattat
    17581 ctaatgtaaa atatttgatc atgattttcc tcctgttgcc tgtttctttt attataacgg
    17641 atagggaatt ggaatacatg gaatgtgttt gtaagatata gaatataact gcataaattt
    17701 aaataaaatg aaacagttgt gtaaagtata tacagtatac ttgaagcaac gataaaaaag
    17761 gagtttacgt tcatgggcac attcacacca cttatcttgt tttggtatat ttacccggta
    17821 atcgttttgt ttggttgcca atttcttgta aaattatttt cgttaaatcg tcgttttaaa
    17881 ataaaagccc ctgatttggc gattcctttt ttgtggggcg gtatgcatgc attgtctaga
    17941 aatacaggga caatttctat cttaccgttt ttctttattt ctgttttgct aatgggaatt
    18001 tgtattgctg ttttccaagc atattattat gaagaaatta tttatccgcg ttatttcaag
    18061 atgacttggc gtttggtttt tctgttgaca atcgtcttgt acgctgtctt gatcattgtg
    18121 aacatacttt cttacctatg aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt
    18181 tttgcagctt gatttttaaa aaaatgttta tgaataaaaa aagttgttcc cggatttccc
    18241 agaaaaggtg gtagaaagtg ggggattgtg gtagactaat tttagttagt ggaggaatgg
    18301 ggggctttca atgttgatgg gtgaattcca acataatatt gatgcaaaag gacgtttaat
    18361 cgtgccctca aaacttcgtg aagaacttgg cgaaaagttt gttctgacac gcggattgga
    18421 tggctgtctt ttcggctatc cgatgagcga atgggaaaat ctggaagcta aactgaacga
    18481 aatgccgttg gctaaaaaag acgcacgtac ttttgtgcga ttcttctatt cggctgccac
    18541 tgaatgcgaa cttgataaac aaggacgaat caatataccc agcacgttaa gaaattatgc
    18601 agctttgaca aaaggttgcg tagtcatcgg tgtatctaac cgaattgaaa tttgggatga
    18661 gacacgatgg caagattttt ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag
    18721 tatgattgat ttcggactat aagaatagga agaggctgac taatgactga aacatttcaa
    18781 cactatacag tcatgttgaa agaaacggta gatggattga acatcaaacc ggatggcgtt
    18841 tatgtagact gtacactcgg cggtgccgga catagtgaat atttattaag ccaactaagt
    18901 tctaaagggc atctgtatgc gtttgaccaa gatcaaaaag caatcgatca cgccaagctg
    18961 cgtttagctt cttatgtcga acaaggtatg gtcacattta tcaaagcgaa cttcagagaa
    19021 ttagaagaac gcttgagtga gcatgtagat aaggtagacg gcattttgta tgatctaggg
    19081 gtttcctctc ctcagctaga tgaagcagaa cgtggattta gttatcatca agatgcaccg
    19141 ttggatatgc gcatggatca gtcagctcct ttatccgctt atcacgtaat taatgaatat
    19201 tcctaccatg aattagtaaa gattttcttc cgctatggcg aggagaaatt ttccaaacaa
    19261 attgctcgtg aaatcgaacg agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt
    19321 gtcgaaatca ttaaacaagt catcccagct cccgcaagaa gaaaaggcgg gcatcctgca
    19381 aaaagaattt ttcaagctgt gcggattgca gtaaatgacg aactcggtgc cgaagaggcc
    19441 tctttagaac aggcaatccg gttattgaaa gtcaatggaa gaatcagcgt aatcacattc
    19501 cattcattgg aagacagaat tgtcaaaagt atgtttaaag aatatagtac ggtacaagat
    19561 ctaccaccgg gacttcctgt catacctgac gaattccaac cagaactgaa agtcattact
    19621 cgtaaaccaa tccttccttc agaggaagag ctaacagaaa acaatcgatc acgcagtgca
    19681 aagttacgta ttgcagaaaa aataagaatg aaagaggagt agaatgatgg cagaattaaa
    19741 gaaaatgcaa gattacgaat atgatttacc tgaattggtg gatcagccgg aacaacaaat
    19801 gcctgattct tcttcgaatc aagaaatcat tcttccacaa tcaccggcaa aacgattaaa
    19861 gcagatttct gctttggaaa aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt
    19921 atgtgtttta acagttatgc tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc
    19981 aatacaaaca gaaatcaatg ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa
    20041 cgaactttct cgtactgaac ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa
    20101 tgacgacaat ttaaggaaag tgaagtaaat gagtcttaaa aataaaattc ggcggtttat
    20161 ggaaaagaaa aatctaaatc cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc
    20221 tacgagtatt ggattgttct ttttatttgc cgtccgtttc tcgtatatcg ttattggagg
    20281 tcatgttgct ggtacctcat tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt
    20341 agtaaaagca aaaagaggaa cgatttatga tcgtaacggt gtggcgctcg cagaagatgc
    20401 ctcctcttat tcaattaaag cgatactctc aaaaacatat acctctgggg ataaaaaact
    20461 gtatgttgaa gaaaagaatt ttgataaaat agcagaaatc ctgcataaga atctttctat
    20521 agacaaaaaa gatgcactga atattttaga ggacggcgca aaaaaagagc tgtatcaagt
    20581 tgaatttggt agctatggga aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga
    20641 catgaaaaaa gaaggtgtcg ctggtcttta ttttgtcgac catcaggctc ggatgtatcc
    20701 aaatggcgtc ttttcttctc attttattgg atacgcagtt ccagacaaag atgagaatgg
    20761 cttagttggt aaactaggtt tggagtctgc ttacaatgat atcttgagcg gtaaagatgg
    20821 taagattatt tatcaaaaag ataattttca aaatcctctg ccaggtacag tggcagaaga
    20881 agaaaaagct gtcgacggac aagatatcta cacgacttta gacagcagac tacaaagtta
    20941 tttagaaaca ctgatggatc aagtaaatga agaatatcag ccagaagaat tgacagcagt
    21001 cctgatgaaa gcaaaaaccg gagaaatctt ggcaatggga caacgaccta ctttcaatcc
    21061 tgaaacaatg gaaggattga ctggaaaaga tgctatctgg cgtaatttct tggtacaaga
    21121 tagttatgaa ccaggatcaa ccatgaaagt tttcactact gcagctgcta tcgaagaagg
    21181 agaattcaac gagaatgaaa ccttccaatc tggaaaaatc caagtagaag atgctacgat
    21241 caatgaccat gactttggtg aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc
    21301 aagtaacgtc gggatggtca tactggaaca gcgattaggc ggacgatggt ataactattt
    21361 acaaaaactg ggatttggac aaagtactca ttctggatta gatgatgaag taaacggtgc
    21421 tttacctaca ttaaatattg tcgaccgagc aatgagtgct tatggacaag cggtaggtgt
    21481 gacgaatttc caaatgatga aagctttcac ttctattgcg aataacggta caatgattca
    21541 acctcgatat atcagcaaag tggtggatcc ccaaactgga gaagagcgaa caactcaaac
    21601 agaagttcta gggcaacctt tttcaaaaga aacaacagaa aaagtccgtg aatatatgag
    21661 agatgttgtc gaaagcgaaa actacggtag tgcttatggt gtatatagtg tacctggata
    21721 caatgtatcg gcaaaaaacg gaacagccca aatcgcttct gatacagggg gatatcaaac
    21781 aggagataca gcctacctgt attcgattgt ggagatggtt ccttctgaag atccagacta
    21841 tgtcttgtat ctaacaatga aacaccctaa gacatatgat cgaatggcat tggctaaaat
    21901 cgcaaatcca ttgatgaaac gggcaatgga ttttaaagaa actgaagaag attcagatac
    21961 agaaacaaaa acagaaaaag tatccgtagc cgattatcgt aatcttgaag cggacgttgc
    22021 agcagctgat gctcaaaaaa gtggactgca acctgtagtg atcggtaatg gtaaaaaagt
    22081 acaaaaacaa tctactgcca acggtgatca gttgatctct ggagaaaaac tgatcctata
    22141 tactggtggt gataagctga tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa
    22201 gctggggaaa atcctcggta ttgaagttag ttttgatgga gatggttatt gtgtcaaaca
    22261 agaacttgct ccatatgaaa aaatcactga agacaaactt agttttactt tagaagaata
    22321 agaagagaaa taggagagaa aagagcatgg aatggacaca agcattgatt cccattgtca
    22381 gcagttgtgc aatgacaatt gccgcaatgc cgctatttat tggttatttt caaatgaaaa
    22441 agcaaggtca ggcgattcgt gaggagggac caaagtggca caatagcaaa gccggaactc
    22501 ctacaatggg cggcttagtg tttctgatcg gctctatttt gacaggaatt tgggttggag
    22561 cttggcaaaa acagctaact cctactcttt tcatcttgct ttttgtatta gcgttatacg
    22621 gagtaatcgg ctttcttgat gattttataa aaatattcaa gaaaagaaac atgggactga
    22681 actcgaagca gaaattgatt ggacagattc tagggggaat catcttttat cttgtttacc
    22741 gttctgaagg ttatccaggt gtattgaatt tcttcggact agatctacct ttaggaatca
    22801 tttatggtat tttcgctctc ttctggcttg ttggattttc aaatgcagta aatctgacag
    22861 atggcatcga tggattagtt gctggcttag gaagtatctc ttttgcagct tacggtctaa
    22921 ttgcttggca tcagcatcag tatgatgtat taatcatttg cttaagtgtt ttaggaggtt
    22981 tactgggttt cttcgtctac aacagaaaac ctgccaagat ttttatggga gatgtcggtt
    23041 cattagcgtt gggcggactt ttagcggcaa tttctataat gctaaaccaa gaatggacat
    23101 tgcttttgat tggtttgatc tatgttatgg aaacagcaag tgtgatgctt caagttactt
    23161 cctttaagct tacaggaaaa cgaattttta aaatgtctcc aattcatcat cactttgaaa
    23221 tgtgcggatg gtcggagtgg aagattgata cagttttttg gttgatcagc attgtgactt
    23281 cgttgatcac attatggatc gtttggtagg agatgacggg aaatgaaaaa aatatcaaca
    23341 tatgaaaata agaaagtgct tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa
    23401 ttattacatg aattgggcgc acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat
    23461 ccggaagcac aagagctgct ttctttaggg ataaaagtaa taacaggtag tcatccaatc
    23521 gaattgttgg atgaagaatt ttcattgatg gtcaaaaatc cagggattcc ctacagtcat
    23581 cctttagtag caaaagccca agaaatgggt attccggtaa ttacggaagt tgagctagct
    23641 tacgaagtag cggaatgccc aattattggg attacaggaa cgaacggaaa aacaacgaca
    23701 acaacgatga caggactact actgaatgca ggagcagatc aaggaattgc acgtttagcg
    23761 ggtaatattg ggtatccagc aagtggtgta gcgcaagaag caaaatcaga agataaaatt
    23821 gtgatggaat tgtctagctt ccaactcatg ggaatcacag atttccgccc tcatatagcg
    23881 gtgattacca atatctatga agcacatatt gattatcatg gtacaagaaa agaatatgtc
    23941 aaagctaaat ggaaccttca aaaaaacatg acagaaaaag attatctgat ccttaactgg
    24001 aatcagtcag aactacaaga attagcgcaa agaacaaaag cacgcgtact gcctttctcg
    24061 acaaaagaag tgttggaaga cggtgtgtat gctgacgatt actcaatata ctataaaaaa
    24121 gaaaaaataa tggaaatatc tgaacttggc gtaccaggta agcataatgt agaaaatgca
    24181 ctagctgcta tttctgtagc aaaattgtat ggtatatcaa atgaagctat acgagaaacg
    24241 ttgcattttt tccatggcgt accacatcgt acgcaatatg taggagaaat ccaaggcaga
    24301 aaattttata atgattcaaa agcaaccaat attttagcga ctaaaatggc tctaagtgga
    24361 tttgaaacgt ctaaggtcgt gttattggct ggcggattag acagaggaaa tacatttgat
    24421 gaattaattc cttcattaaa aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa
    24481 aagttgatgg atgctggaaa aaaagcaggc atcgagacaa tagtaacagc cgattctgta
    24541 gaacaagcag ttcctttagc attagaaaac agtacagatg gtgatgtagt tttattatca
    24601 ccagcgaatg ccagctggga tcaataccca aattttgaaa ctagaggaaa cagattcatg
    24661 gaagctgtga accgattaaa atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt
    24721 ggttacaggc ggaggaacag ggggacatat ttatccagct cttgcctttg tcaattatgt
    24781 aaagactcaa gaaccgaatg ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa
    24841 caaaatcgtc cctgatacag gaatgccttt ccataccttg gagattcaag gatttaaacg
    24901 aaaaatttcc atgcataaca tcaagacggt ccgattgttt cttaagagta tccgtcaagc
    24961 aaaaaaaatc ctcagtgatt ttcaaccaga tatcgttatc ggtactggag gatatgtttc
    25021 cggagcggta gtgtacgctg cagccaaaat gggtattcca acagttatcc atgaacaaaa
    25081 cagtgtacca ggtgttacca ataaattttt aagccgatat gtggatcgga tcgctctttc
    25141 atttaaagat gcggcttcct tttttcctga aaacaaagca gttcttgtag gtaatcctcg
    25201 agcacaagaa gtagcagata ctaaaaaatc agatattttg aaaacatttg atttggatcc
    25261 agaaaagaaa acagtcttga tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc
    25321 agtatcagtt tttttaagtg aatatattcc ttcttcttac caagttttgt atgcttcagg
    25381 agatagatat tatgaagaga tcctaaataa aataaatcct gaaatacaaa atattagtgt
    25441 ccagccttat atcaaaaata tggcagaggt catggcttcc agtgatctgc tggtggggcg
    25501 tgctggtgca acttcgatcg cagaattcac agctctgggg ttaccggcag tgttggttcc
    25561 cagcccatat gttacgaatg atcatcagac taaaaatgca atgagtcttg ttcacgcagg
    25621 agcagcaaaa atgattgcag ataatgaact aacaggtgaa tcactaagtc agacagtcaa
    25681 cgaaatcatg ggtgatgaag aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg
    25741 aatccctgat gcttcaaaac gtctttatga cttagtaaaa caaataatca aaaaataaag
    25801 gcggtggtct tagtgagtaa gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat
    25861 gaaaacaatt tgactccttg gcaaaaggct aaccgaaaat atctggcaga acaagagatg
    25921 aaaaaccaag agccaaaaaa agaagacata acattagata cacccctttc agattctgaa
    25981 gaagaaaaaa cggttcaaga ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa
    26041 ttttttgaag agatcaagca aaagaaaaac ggtggaccat ataacggttc ttttttgaac
    26101 cggctgccca atttaaaaag tcaacggcac aaagtactat ttcgtcggtt agctttgatc
    26161 atcactgtat tgggtatacc gctgattttt ttgatctatt atgtttctcc ttatagcaaa
    26221 ttacaagcag ttgcagtatc aggcaataag acagtcaaca gccaagaaat catctctgat
    26281 acaaaattgt cattaggaga aaatgtttgg ggacagtatt ttcatcgatc tacttatatc
    26341 gatcgcctta aaaaagcaca gccaagaatc gaaacagcaa atatacattt caaagggatg
    26401 aatgaattcg atttggatgt aacagaatat aaagagattg ctttgatcgc aaaaaacaat
    26461 caatactacc cagtgattga aaatggaaca gtattagatg aaaaagtagc aaatcctaca
    26521 aaaaatctcc ctatcttgga agagttcaaa gactctgcaa aaatcaaaga attaacaaag
    26581 caatacaatc agttatcatc agaattacaa aaagcaattt ctgaaatcaa gtacacgcca
    26641 agagcaagca ataaaaatct gatccaattg aatatgaatg acggcaatca agttattgta
    26701 aatatcaata atcttgcgaa ccaaatgaaa tattattcgc aagtagccaa agatatggat
    26761 gaaaaaggtg tgatcgacat ggaagttggt atcttctcgc atccttacgg tagcactcaa
    26821 gaaacggaag aatcgggcca atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa
    26881 acaaacgaat caagtgaaga ggcagaaacg caaaaagcac aaacacctgg atcgtctagc
    26941 acaaacggat catctgattc aaatgattca agtgaaacaa gtcaaataga agataatcca
    27001 agtacaactc cgtcaagttt aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt
    27061 actttctaaa aaccagtaat ttatggtaga attggaagca gtgaatatat ctattaataa
    27121 gtatatttta aaacggacgc tagttgttcg cttgtatatg gaatagtaag taggagggaa
    27181 tcccattcat ggcaaaaaca ggaatgtatg taggccttga tattggaacg acatctgtta
    27241 aagttgtcgt ggctgaatat atcgacagcc aaatgaatat tattggagta ggaaacgcaa
    27301 aatcagaagg gattaaccga ggcattatcg ttgatatcga taaaacggtc caagcgatac
    27361 aacgagcagt acgacaagca gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg
    27421 gtttgccagc aaacatgctt gaagtagaaa attgccaggg aatgattgca gttaacggag
    27481 attcaaagga aatcaccgat gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt
    27541 caatacctcc tgaacgtcaa atcgtgtcaa tattgccaca agattttact gtcgatgggt
    27601 tcgagggaat caaagatcct cgtggaatga tcggcgtaag attagaaatg tacggtctgt
    27661 tgtttacagg acctaaaacg atcatccata acatacgtaa atgcgtggaa aatgccggat
    27721 taatcgtcaa cgaaatggtg attactcctt tagcgctgac tgaatcaatt ctttctgacg
    27781 gagaaaaaga ttttggtacg atcgtcattg atatgggtgg cggccaaaca acaacggctg
    27841 ttatgcatga taagcaattg aagtttacca atcttgacca agaaggtggc gaatttgtca
    27901 ctaaagatat ctctattgta ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa
    27961 actacgggga tgcataccca gaacggacat caccagatga agaatttcca gtagatgtga
    28021 ttggtcagtc agaaccagtg aaagtcgatg aacgctactt gtcagaaatt atttcagctc
    28081 gaatggaaca gattttcaac aaagcaaaag atgcattgga ccaaatcgaa gcattagagt
    28141 tgccaggtgg cgtcgtattg acaggcggag ctgctagtct tcctggtgtt gtggacttgg
    28201 cacaagaaat cttcggcgta aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa
    28261 acccggtatt tacaaatgta ataagtatcg tggattattc agcaaacttg agtgaagtgt
    28321 atcaattggc aaaaattgct gttacgggtg aaacctcagc agctcgtcaa atggtagttg
    28381 aacaagaaac aacaaacaca tatgagagct atgaagcacc tgaagaaaca atctacgatg
    28441 agccggaacc aaaagagtcc ggagaaaatg taaaaaataa aatcaagggt ttctttacaa
    28501 atatttttga ttaattcatc ctgggaggaa taagatatgg aattctcaat tgataataat
    28561 atcaatgacg gcgcagtaat caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta
    28621 aaccgaatga ttgaagaaaa cgttaaaggt gttgaattca ttacagccaa cacagacgtg
    28681 caagcattaa aaaattcaaa agctgaaaca gttatccaat taggccccaa atatactcgt
    28741 ggtttaggtg ctggttctca gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa
    28801 tccttacgcg aagctttaga cggtgcagat atgattttca tcactgcagg aatgggtgga
    28861 ggaaccggta caggtgctgc accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg
    28921 actgttggtg tcgtgacacg tccattcact tttgaaggac cgaaacgtgg ccgttttgct
    28981 gcagaaggta tcgctcgttt gaaagaaaat gtggatacat tattaattat ctcaaataac
    29041 cgtttattag aagttgtaga taaaaagaca ccgatgcttg aagctttccg tgaagcagat
    29101 aatgttttac gacaaggtgt acaaggtatc tctgatttga tcacagcgcc aggttacgtt
    29161 aaccttgact ttgctgacgt gaaaacagtg atggaaaacc aaggtacagc attgatgggg
    29221 atcggtgtgg ctagtggaga agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct
    29281 ccattgcttg aaacatctat tgatggagct gaacaagtac ttcttaacat cacaggcggt
    29341 ttggatatga cattgttcga agcacaagat gcttcagata tcgtagcaaa tgcagcaact
    29401 ggcgatgtaa atatcattct aggtacatct atcaatgaag aaatgggcga tgaaatccgt
    29461 gtgacggtca tcgcaacagg tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca
    29521 gctagacaag cacaaatgca atcacctgct caaaagactg tattagatat ggatcaagct
    29581 aaaccaactt cttcagaaga agaaaacagt tttggcgatt gggatatcag acgtgaacaa
    29641 aatgttcgtc ctagagttga tgattccaat tttgacaata tcgaaaaaaa agaatttgat
    29701 acattcaacc gcgaagaaac aaaatctcat ggcgatgatg aattaaatac gccaccgttt
    29761 ttccgtcgta aaaaataaag gagaggaaga gcacatgatt gctgacaact tgcagaaaat
    29821 caagcaagaa attcaggatt cgtgtgctct tgttcatcgt gatccgaaag aagttacatt
    29881 agtagctgta acaaaatctg ttgaatccgc tcaagctgaa gaattggtta gagctggcgt
    29941 tatgaattgt gcagaaaacc gagtagataa attattagaa aagaaacaag atttatttga
    30001 ttttcctgaa ataaagtggc atttaattgg taatttacag cgcagaaagg taaaattgat
    30061 tgtaaatgaa attgattact ttcatgcgtt ggacagttta agattagcag aagaaatcca
    30121 gaagcggtta gagaaagaat tatcctgttt tattgaagta aatgtttctg gtgaggaatc
    30181 aaaacatgga atttcaccag atgagctttt accgtttgtg gaatctttag ctgcctgcac
    30241 aaaaatcaag gttgttgggt tgatgactat ggcgcctaaa gaagcttctg atgagcaaat
    30301 ccataaagtg tttggtacgc tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc
    30361 ccatgcacca tgtagtgaat taagtatggg gatgagtcaa gactttcaaa ttgcgattga
    30421 agaaggtgca acatttatcc gtgtaggaac agcgctattc aaagacgaat aaaggaggaa
    30481 caaaaatgcc gcttttaaac agaggagcaa ttgctaattt tttcggatta gctgatgagg
    30541 aagagtacga ataccaagat tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa
    30601 aaaatacaac acagaatcaa tcaactcatt ctgtatctgc tgcgaatact catgaaaaac
    30661 aaaaaacggc gacttcacgt cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa
    30721 aagtaattga actacatcaa tcacctacaa aaactgtagc gaaagaaaga acagcttcaa
    30781 gaacctctac acaacctaaa gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag
    30841 cttattctga agctatggcg attgctaaac gaatcattgc tggagaagca accttagtga
    30901 attttcattt agtagaagaa aaacaagcga gaagaatcgt tgattttcta acaggaacag
    30961 tttatgcact agatggagat atcaaacgtg ttggagatga gatttttctt tgtacaccga
    31021 ctgggatgga gattgacagt tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc
    31081 tgtaagagga ggaactacga tttatactat tgcactatta cttcatttac ttcaacgggc
    31141 agcctatttc tatagcatta tcttgatggt atatgcattg ctctcttggt ttccaggcgg
    31201 gtatcaatca gggctaggac gattattagc taagatctgc gaaccgtata tcagtttgtt
    31261 cgatcgtctg cctttgagga ttggaccctt ggatttcaca attttagggg caatcctgat
    31321 cttgaactta gccgtacaag gtttagcaca gatcgttata tggataactg tccatttagt
    31381 ttattgattg aagcaacaaa atctttattc tcagaataga taggagggga aaattttgga
    31441 tgcaaatgta taccagcact ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga
    31501 ttggattgaa caggttcaag gacaatatgc accctatctg accgaatttc ttgacccaag
    31561 acaatcctat attttagaaa cactgattcg acaaagcagt gatttgcgtt tcatgtttta
    31621 tggcggatat gaacaggcag aaagaaaaag atgtttgatt ttccccgatt attacgaacc
    31681 tgtagaggaa gattttgaaa cagcattgta tgaaatccat tatcctagca aatttgccag
    31741 cctaagtcat gggaaaatcc ttggaacttt agtgggaacg gggatcaaac gctcttactt
    31801 tggcgatatc atctcggacg gtgatcgctg gcaagtattt attgcaaatg aggtggatca
    31861 ttttgttgct gcacaagtaa caaagattgg aaaggttgct gtacgcttgg aaccaatcaa
    31921 atacacagaa atgattatgc cgaaagatag ctggacacaa gaaagaggaa cagttagttc
    31981 catgcgacta gacagtgtga tctctgagat ttataatatt tccagacagc ggtccaagca
    32041 gctgatcgaa gctggtaaag taaaagtgaa ctggacagaa aatacaagac ctgattttgt
    32101 acttgaacta ttagatatta tttcagtcag aggttatggc agagtccaga tccaagaact
    32161 tgaggggaag acgaaaaaag aaaagtacag ggtactgttc ggtgtattac gtaaataaaa
    32221 taaaaaggtg gtttactcat ggctttaact ccattagata tccaaaataa gacattcccg
    32281 acaaaaatgc ggggatataa tcaagacgaa gtagatgatt tcttagattt agttgttcgt
    32341 gactatgagg aattgactca aagaaatcga gaattagaaa aagcagtcaa acattctgaa
    32401 gaaaaacttg agtacttcaa tgaattaaaa gatgctttga accaatcaat catcgtagct
    32461 caagatacgg ctgataaagt aaaaacaagt gcaagcaagg aatccgaagt gatcgttact
    32521 tctgcacaaa acaaagcaga tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta
    32581 acaactgatg cagaagaaaa agcaagaaaa atcttgacag atgcaacaga aaaagcaaga
    32641 cagcttgcga cagaaactga agatcttaag aaaaaaacac gagtattcca tcaacgtatc
    32701 agcttgatgc ttgagtctca attagaacaa gtgaaaagtc cggaatggga tgaaatttta
    32761 caaccattct ccagctacgt gactgacagc catgaagtga tcaaagaagt tttagcaaaa
    32821 cagcttgaca atgaaaatga tactgatgta aactcagaca caggagtcat agaagcacct
    32881 atgacggcat ttgatacaca ggctattgat atctcgatcg tgcctggtga aaatgaaaac
    32941 gagtagaaca gaaaagcact ttgttattct taaagcgagc cgatgagagt ggaagttcgg
    33001 taagacactg catggtgaaa gatcctctac ttgagacctg cttttccgaa attagagtaa
    33061 gaagcagcgg ctgacctcgt tattggtcac agagagtttg cactagatag atgcaactaa
    33121 atttgggtgg taccacggca tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg
    33181 aaaacaaata aaggagtgta tggttgaatg aaaatgaaag aaaccttgca attaggaaaa
    33241 acaaaattcc ctatgcgtgg taatttgcca aaccgagaag gcgagtggca aaaagaatgg
    33301 gaagaaaatc gaatctacca aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt
    33361 cttcatgatg gacctccata tgccaatggt aatatccatt taggacattc attgaataaa
    33421 atcagtaaag acattatcat tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt
    33481 ccaggatggg atacacatgg attgccgatt gaacaagtat tggctaataa aggcgtgaaa
    33541 cgtaaagaaa tgtccatggc agaatacttg gaaaaatgtc gtgaatatgc actttcccaa
    33601 gtagataagc aacgagcaga tttcaaacgc ctaggagttg caggagattg ggaacatcct
    33661 tatgttacat tagatccttc ttatgaagct gcacaagtac gcgtatttgg taaaatggct
    33721 gaaaaaggct atatttacaa aggattgaaa ccaatttact ggtctccgtc aagtgagtct
    33781 tctttagcgg aagctgaaat tgaatataaa gatgtaaaat ctccatctat ttttgtagct
    33841 ttcaatgtca aagacggcaa agatattctt gatgaagata cagcttttgt catttggacg
    33901 acaacaccat ggacattgcc agcaaaccaa ggtattgctg taaatccaaa atttacgtat
    33961 gtattggtgg aagccgatgg ccgtaagttt gtcgtagcga aagatttgct agaaacagtc
    34021 caatcagaaa tcggttggga aaatgtaaca gttctaaaag aatttgctgg acaagaaatg
    34081 gaatatatga ctgcaagtca tccattctat gaacgtgaat cacttgtgat tttaggcgat
    34141 catgttactt tagacgctgg tactggatta gtccatactg ctccaggaca tggggaagat
    34201 gactatatcg ctggtagcaa atataaacta tctgtagtgt cacctgtaga ctcaaaagga
    34261 ttgtttacag aagaagcacc tggatttgaa ggtatctttt atgacaaagc aaatcctatg
    34321 atcactgatc tattaaaaga aaaaggagct ttgctgaagc tagatttctt tactcatagt
    34381 tatcctcacg attggcggac gaaaaagcct gtgatctatc gggcaactcc acaatggttt
    34441 gcatctatcg ataaattccg tcagaatatt ttggatgaag tagaaaaagt agagtgggtt
    34501 atcccatggg gtaaaacacg tttgtataat atgatccgtg accgcggcga ttgggtaatt
    34561 tcccgtcagc gtgcatgggg agtaccactt ccgattttct atgctgaaaa tggagaagca
    34621 atcatcacac cagaaacaat cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt
    34681 atctggtttg aaaaagaagc gaaagaactt cttcctgaag gatttaccca cccaggttct
    34741 cctaatggcg aatttacaaa agaaaaagat atcatggatg tctggtttga ttctggttct
    34801 tctcatgaag ctgttttacg tcagcgacca gaattatcat tcccagcaga tatgtattta
    34861 gaaggatcag accaatatcg tggctggttc aactcaagta tcacgactag tgtggctatc
    34921 aatggtgtag caccatacaa atcagttatt tctcaaggat ttgctttaga tggtgaaggc
    34981 cgtaagatga gtaaatcttt aggaaacatc attgcaccag aaaaagtgat caaacaattt
    35041 ggtgcagata tccttcgctt atgggtatct agtgtagatt atgaagcaga tgttcgtgtt
    35101 tctatggata tcttaagtca agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt
    35161 ttcttattag cgaatacaga agatttcgat cctaaaaaag atactgtttc gtacaatgat
    35221 ttacgttctg tagacaaata tatgaccgtt cgattgaacc aagtaatcaa agaaatccga
    35281 gaagaagggt acgacaaata taacttcatg catatttacc gtacagttat gaacttcttg
    35341 acggttgatc tatcgtcatt ctacctggat tttgctaaag atgtagttta tattgaagca
    35401 gaagatgatt atcaacgtcg ttgcatgcaa actgttttct atcaaacagc tgtagctttg
    35461 acaaaattat tgactcctat cattccgcat acagcagaag aaatctggtc attcctgaaa
    35521 gaagaagagg agtacgttca gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa
    35581 gaagaattga tggatacctg gactgctttc atggacttcc gtgataatgt cttaaaagca
    35641 ttggaagaag caagaaacag caaattgatt ggtaaatctt tggaagcaaa actaactgtt
    35701 tatccaaaac aacaagttcg tgaaatgtta aaagctttgg atgcagatat tgcacaatta
    35761 ttgatcgttt caccagacta ctttgaagtt atggcagcag atgaacaagt gccagataac
    35821 gctatggtat ttgatgatgt tgctattaca gtggaaaaag cagacgggga aacttgcgat
    35881 cgctgtcgcc aagttcgtaa agatgtagga gtagatgaaa aacttcctca tctatgcggt
    35941 cgttgtgcaa aaatcgttga agacttctat cctgaagctg tagctgaagg attcgaagaa
    36001 aagtaaaagg gaaaaggggg tgtgacatgt tctttgtcac accccctttt gttatcaatc
    36061 gtttaatttt ccacgttcac gataccagtt atctggttgc cagatccatt cgaatccgtc
    36121 tttttcaagt agttcgaaag ctgctttagg tcccattgat ccagctttgt agttgggaaa
    36181 atccggttgt gtttggtccc aagcttttcg aatgacatcc acgatatgcc aagattgagc
    36241 gacttcttcc caatgagaga agttcgttcc atcaccattc aacgcatcta atagtaattt
    36301 ttcgtaagct tctgggctat tttcaaccat ttctgcacta tttctaaatt ccaatttgat
    36361 tggttccgtt tcaaatcctt gccctacttc tttcccattc aaggataaag aaaatccttc
    36421 agttggctga atatagatcg ttagaacatt aggtggaagt gtcgtatcgt cacatggttc
    36481 atcgacagat gttttgaata cattaacagg aacttgtttg aatacgatgt tgatccgagt
    36541 ccctttttca gtcaaacgtt tgcctgtacg tacatagaaa ggaacacctg accaacggaa
    36601 gttatcgata gtgaatttgc ctgcgacgaa tgtttcagtc tctgattgag catccacttt
    36661 gtcttcttcg cgatagccct tgaattgtac accatccaat tcaccttgag catattgccc
    36721 gcgaacgaag ttttctaaca cttcttcttc gttataaatg cggatagctt tcaatgcttt
    36781 gatcttttct gtacggattt ctttttcgga aaatgctact ggcggttcca ttgcaagtaa
    36841 tgctacaacc tgtaaaatat gattttgcac catgtcttta agtgcgccac tatgatcgta
    36901 gtagcccccg cgatcttcta caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat
    36961 atagcgattg ttccattgag cctcaaaaat attattggca aaacggatag ctgagatgtt
    37021 ttggatcatt tcttttccta aataatgatc aatcctgaaa atatcttgtt ctgggaatac
    37081 ttctctgatt tgctggttca attcaaaagc agactggtaa tcagaaccga acggtttttc
    37141 tatgatcaaa cgattgaagc cattttttgt cataatattt tgtgatttca aatgagaaac
    37201 gatcgttcca aaaaattgag gtgccatagc caaataatag acacggttac cgtttaattg
    37261 atagtgtcca tctaatcggt cagacaattc ttttaatgtc tgataatgcg ttgaatcatt
    37321 tacatcatgg gattgatagt aaaaatggct tgcaaaggta gttgcttctt catctgaagg
    37381 attcaatgca cggattgttt ctttgacaac ttcacggtag tgctcatcgc tccagggacg
    37441 gcgagcggtt ccgattacag caaaatgctc gcctaaattt ccttttctgt aaaggcggaa
    37501 caaagacggg tacaatttac gctgggctag gtcacctgta ccgccgaata tcgtaaacaa
    37561 tacatttttt tcattagaca tgtggataca ctcctttata ttttatcgac aaaaatcgtg
    37621 ctggccgcct taaagctgac ggggattttc tttctatcat tttggatagt gatcggtcct
    37681 tcataagctg cgatgtcaac gatctcatat tcttcgttga tattcaggtc aatagtgact
    37741 aagtaatcca acaattcttt ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca
    37801 ggataggact ccaatgtttg tcggcgttct tcatggacaa cttcctcttt tttaggaatc
    37861 gatcctccat gaggacaatg ttcaggatga ttcaaatagt tttctaaatg atctgcaaga
    37921 tgttccgaag tcacatgttc caatacttca gcatcatcat ggacttcatt ccagctatag
    37981 tctaaatgtt caactaaaaa aacttcccat aagcgatgtt tgcgaatcag actgctagct
    38041 ttttgcagtc cgcttgcagt caattgtaca ccttgataag gggaatgttc tacaagctct
    38101 tctttcacaa gtttagaaat catttcactg acagatgcag ctgagacatc tagactagat
    38161 acgatttgtt tattgttgac tttcgttgta tctccaccta gttctaaaat gattttcaaa
    38221 taatcttctc gatttggtgt catattcgat catcctttca tggtaaaaaa ccatcattag
    38281 taagtgtaac aaaaaaaacg ttaattgact atttttctaa ataaaaaaca aaggattttt
    38341 agacgttttt acaacaaaaa acgaaagatg atagagaaga ctcattcttt cgtttataaa
    38401 ggatatatat atacattggg agggatctat ttttcagata acaacttata tgaagtgtct
    38461 gcagattcgt cataaagagt ttgttcggga taagaaaaga taagataaaa accatactga
    38521 tcacgtacga ctaaacgata ttcatctatt tctaccacag atgtgccaag agactgaccg
    38581 ttgatcaaaa acgtgtaatc agatgtaatc tgtaattgtt gtttttgctg gctatcacta
    38641 aagaaccatg taccttgata ctttgagagt ccttctgact tttccgcttg ttttttcttt
    38701 ttcaagtaaa taaaacttga gagaacggta atgagtgaac caagaaaata gatcattttt
    38761 tttatcacca aatcactcct ttatgagaaa ataatcgcat agacatattc tttcttcttt
    38821 atttcattgt accgaataaa agctaaaatt tctctaagac tttcgactgg tttttttact
    38881 gtttttctac aaattatgat aggatacata gtaggaagaa aggtgacata atttgaagta
    38941 tcagcaagtg gaaatagctt tttttattga ccgcccgaat cggtttatag cacattgtat
    39001 gaaaaaagac ggagaaataa ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt
    39061 cctgatacca ggcgctgaag tggctattgt atttgctcct ggacctaaac ggaaaaccgc
    39121 ctatgatctg attgctgtaa aaaagaaggg tgactggttt aatatcgata gtcaattgcc
    39181 taatcggctg gcaattgatg gtattttaga cggcacgatc cttttaccag gattaactgg
    39241 tccatttacg gtatacaagc gtgaagtaac ttatcttcac tccaaatttg atatttattt
    39301 agagacggtg gtcggacaaa aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa
    39361 agcgattggt gcttttcctg atgcgcccac actcagagga ttgaaacata taggagaatt
    39421 aacgtatgcc gcacaagatg gttatgccgc ttatgtcttg tttattgctc agttcgaaca
    39481 tcttcatctg gcaacgatcc atgaagaaat gcaaccagca ctagctgata tggttcgaca
    39541 tgcgcagcaa agcggagttc aaatcctcgc ctataattgt caagtatcgg cagatgaggt
    39601 aactgtcaag caggccattc catttgatgt agatatccct tttgaagatc ctatacaata
    39661 gaaagaaggt aaaataatga tcaacttagg tattatcgga acaaattgga ttactcatca
    39721 atttgtccaa gcagctttaa gcacaaaaaa atatgaattg acagcggtat attctagaca
    39781 tttggaaaaa gcacaggaat ttggttctca ttatgaaggg gatatcgaat tcgcaacaga
    39841 tttgaagact tttttcaata ttactcatat ggacaccatt tatattgctt ctccgaatag
    39901 tcttcacttt agtcaagcta agcaagcaat tttggcagga aaaaatgtga tagttgaaaa
    39961 gccagctttt tcgacgccaa aagaaatgga tgaaatcatc catttggcaa atgaacacaa
    40021 tgtgtatttt tttgaagcag caagaaatat ccatgaatca ggttttaaaa agattgccga
    40081 ttacttacca ttaaaagacg agataatcgg tgcaaatttc agctatatga aatattcttc
    40141 gcgctatgac caagtattag atggagaaga accgaatatc ttctcgactc atttctcagg
    40201 tggtgcattg atggatttag gtgtttacct tgtatatgca gctgttgctt ggtttggaat
    40261 gccaaaagaa gtccattatt tcccaagaaa gatttctact ggtgtagatg gtcttggatg
    40321 ggggatctta cgttatgaga attttgatgt atcgatccag ccaggtaaaa tcggtgattc
    40381 ttatttaccg tcagagattt attttgactc aggaacgcta gtgatgaatg gagtcaatgc
    40441 aattgaacaa gcacaatttc atgatcgcgc gcataaagaa atcattgatt tggcggtaac
    40501 agctgcagaa aatccaatgg cagatgaagc agaggatttt gccaatgtca tggctcatcc
    40561 gaaagatcca gcttggggat tgttgtatga agagtggatc gagctagcta gaaatgtgaa
    40621 ccaagtgatt tatgatttaa gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga
    40681 agatttttaa tgaacgaatt tgtacaagta tttcccgaaa attggcagaa aaaatatgag
    40741 gataaagggt ttatctcacc ttctcttatt caacgagcag tttttcagcc actaagtaat
    40801 aaacaaagta tagtagcggt ttctcctaca ggttcaggta aaacactggc ttatctttgg
    40861 cctctactac taaacgtgga accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct
    40921 caagaactag ctattcaagt agcagatgtt gcacgtgaat ggggaaaaga caaagagttg
    40981 aaagtccaat ctttggtggg cggagccaac gtcaaacgtc agattgaggg tttgaaggaa
    41041 aaaccagaaa tcttgattgg tacaccagga agaatattgg aattgatgaa agcaaaaaaa
    41101 ataaaagcac atcaagtgaa aacaatggta tttgatgaag cagaccagct atttgacgct
    41161 gggaacagcc aaatcattga tcaaattctt catcaagctc caacagagta tcagctagct
    41221 tttttttcag cgacagcaga tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg
    41281 tttgagacaa ttgacgtgac agcagaggac gattctcgaa aaggactgag acattacttt
    41341 ttacgtgtac cgatacgaaa gaaagatgac tacttgagac gtctcacgca tatagaagat
    41401 tttcatggtc tgattttctt taatcaactg actgaattag gcgtaatgga agaaaaattg
    41461 ctgtatagag gtgtaccggt aggaagtctg gcctctgatc agaacaagct actccgaaaa
    41521 gcagcgctag aacaattcaa aaagaaaatg gtcagagcac ttttgaccac agatgtggcg
    41581 gctagaggtt tagatattac aggtcttcct tatgtagtga atgcagaagt tccgttaagt
    41641 gaagaagctt atttgcatcg ttcgggaaga actggtcgaa tgggtaatga aggaatagta
    41701 attacactcg tacaagataa taatctaaga gatttaaaac ggcttttacg tccatcaaat
    41761 ataacacttg aagagatttt tttatacgga ggtaagctgc aaactgagca gccaaccaaa
    41821 gaagatgcag ctcctgcagt aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag
    41881 aacccagaat ataacaaaga aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag
    41941 agaaataaag gagcaaggcg caaataagcg cctttttatg ctatgattat gaaaacggga
    42001 acaaaattgg ttgccagagg aggggttatg tgaaagaacg tcaagaagag atcttgcagc
    42061 tattgacaga aaaccaacaa ggattgacag caagtgatgt agctgagcgc ttaactatcg
    42121 atcgaagcaa tgcaagccga tatttaagtg aactatacaa ggcccatcat attgtaaaaa
    42181 ccgctggccg accagtcgtt tactctttgc caacggaaaa gtcaaaatct gatgaagtgc
    42241 atgtcgactc ttctacgcaa gtgacttttg aaacactcgt tggagaaaat gattctttaa
    42301 aagtcagcat acagcaggca aaagctgcga ttctttatcc accgcggggg ttgcatacga
    42361 ttatttttgg agaaactggt acagggaagt cgatgtttgc tgaatgtatg tatcattttg
    42421 caatcgattc agagatgctt tctgcagatg cgccatttgt ttctttcaat tgtgcggatt
    42481 atgctcaaaa tccgcaactg ctctttggac acatctttgg gatcaaaaaa ggggcttaca
    42541 caggagcagc tcaagatagt cccggtctaa tagccaaagc agacggcggt atattgtttt
    42601 tagatgagat ccaccgttta ccgccggaag ggcaggaaat gctgtttact ttcattgata
    42661 aaggtattta ccgtccgctt ggagaaagtg cacaagtaca tgaagcatct gttcagatca
    42721 ttggggcgac gactgaatct tcagaaagtt tcttatctac gttcaatcga cggattccta
    42781 tggcaatcac cttgccgaat cttgcttcac gttcactgga tgagcgttat gaaatcatct
    42841 ctctttttat caaacaagaa gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag
    42901 cgattcttgc cttcatgctt tatgatgcgg aaggaaatat cggtcaagtg aaacgagact
    42961 tgaaactagt ctgtgcaaaa tcttttttac attaccgtac tcatcatgag aaaaagctaa
    43021 tcattcgaaa agaagaactt tcccttcaag tacaaaaagg attactgaaa atcaaggaaa
    43081 taccggaacg tctcgaccga ttcatggaca gcaaaagtca gtatttgaca tttgaaccag
    43141 gatttgctga tgttgtctgg tcacaagatc cagaacgaaa catgcaggtc tataacgata
    43201 ttgaggaaaa agtcacttct ctttccgaaa ccggtgtgga gaacattgat ctagagaccc
    43261 tcatttcaaa agatgtggat gcttatttcc agacatatgt caaagaattg actaaaagtt
    43321 cgattcccaa agatcttttg ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg
    43381 tggctgaaaa acggttagac cgtatttata atgaaaaagc tcgttttgct tttgctttgc
    43441 atctgcaaag tacattggat cgtgtgagag aaggtcatat gatcgtccac ccagatttga
    43501 ataacgtacg gaaaaatttt aagagggaat tccaggtagc aatcgatctt tcaacgatca
    43561 ttgaagaaga agaacaagtt gagattcctt ttgatgaaat cggctttatt agtatgtttc
    43621 tttctatcaa tctaggagaa agtgagcctg ttaaagaaaa caaagtagat gttctcatat
    43681 tgatgcacgg cagacagact gcttcaagca tgttggagat ggcacaagaa ctattaggaa
    43741 caaaagtagg gaaagctttt aatatgccac tggttatgga agttcaaaca atgtatgaag
    43801 aagtgttaca atatgtcaaa aactatcaag agcatttgac gaatggccta ctcttgttaa
    43861 cagatatggg atcattaaac accttcagtg atttgattta tgaagagact ggcattcgga
    43921 cgaaagctat cacgatgaca agtaccatga ttgtgataga agcgatacgg atggcaagtg
    43981 ttggaagaag cttagaagat atttatcaaa atatccagct ttcctttgaa tcgatcgtac
    44041 gtgatcagtt caaagcggaa aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga
    44101 catgctttac tggtgaagga gtagctgcca aattatatca gcgtattatg ccagtgatta
    44161 atcaaaataa ggtagaactg atccagatgc agtttttaga acgtgaatca ttcaaaaaac
    44221 acatcgatgc cttattggaa gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg
    44281 attatcaaaa tatcccattt ttttcagcct atgacgtttt tgatgatgaa aaactaaata
    44341 ttttaaagcg tatagctgat gatgagatcc cgacagctaa aattgtcgat tcacttgctg
    44401 gatcattaag cgatgtcttt tctattgatg aactgatccg ttcattgcaa aagacagttc
    44461 agcagatcca gaccgatttg tcgattatcg tggaacctgg agtagatact ggaatcgtta
    44521 tccatttagc cttcatgtta gacggattga tcaaacaaga aagccatcgg caatttgaga
    44581 aactgtctat tttctcaaaa atttatcgtt tggaaatgga tatcgttcgg gcaaacttga
    44641 tggcactaga acgaaaatat caggtaaaaa ttccagaaga tgatatcgca tttcttacac
    44701 aaatgtttct tgaaaataaa ataaaaaaat gaacacacta atcatactgt ttacacacta
    44761 aacatctgtg tgcgcactat aaaaaagagc taaaaagcat gaaaacgcta tttcagctct
    44821 tttttttatt ggcatgcttc ttgcgttata taaaagtgta aaaaaataaa aggagtgtta
    44881 aaaatggcaa aaaaaacaat catgttagtt tgttcagcag ggatgagtac gagtttattg
    44941 gtcacaaaaa tgcaaaaagc agcagaagaa aaagggatgg aagccgatat ttttgcggtc
    45001 tctgcctcag atgcagataa taatttagaa tctaagaatg tcgatgtatt attattaggt
    45061 cctcaagtac gtttcatgaa agctcaattt gaacaaaaac ttgcgcctaa aggtattcca
    45121 ttagatgtca tcaatatgca agactatggc atgatgaacg gagaaaaagt tcttgcacaa
    45181 gcagaaaatc taatgaaata aatcaagtat gcagttgggg gtataacaac gtggaagatc
    45241 aacaaaatct agaagccatc atggggttaa tcatgtttgg cggtaatgca aagagcgatg
    45301 caatggaagc gattgcggct gctaaaatcg gtgattttga gttagcggat aggaaaatcg
    45361 ctgatgcaga agaatcactt atacaagcgc atcatgcaca aacagacatg ttgacacaag
    45421 aagcacaagg aaatcatatg caagtgacat tattgactgt tcacagtcaa gatcacttaa
    45481 tgacatctat tgcgtttact gatttagcaa aagaaatcat cgaactttat cgtcgcgtat
    45541 acagcgaata aatacaactt cttttttgaa cggaagaaga aaaatttttg aacggatttt
    45601 ttattttgtt ataaggagaa ctctctttat ataaaaaact tataggagga agtaaaaaat
    45661 gaacggacta tcaagttgga tggagaaata catcctgccg gttgccgcaa aaatcggagc
    45721 gcaaaaacat ttagttgctt tgcgtgatgc ttttatcggc acactgcccg caacaatggc
    45781 gggatcagtc gctgtaatga tcaatgcaat tattcgtgat ttaccgcaac agtttaacag
    45841 tacctatgct gacagtttag cagcagacaa aggaatcttt gccgtattga atgtcatcat
    45901 cggaatcaat ggttttgtct ggaacggtac actagcaatc gccggattga tctttgcctt
    45961 ttcttggggg tataatcttg cccgagctta tggagtaaat gatctagctg gggggatcgt
    46021 tggtttagct accttgattc aaggtatttc ctttggtcca gcattatcag cagcattaga
    46081 tgtgaatgtt ccttcaaatg tcgcagatgc aatcaatagt tctgagattg gtgcaacgat
    46141 tgcagatggc acactgtcag ttggattatg gggttggcta aaactagatc atttgaatgg
    46201 aaacgcttat tttacagtta tgatcatggg agctatttca gtcattattt tttgtaaatt
    46261 gatgcttgcc aacattacga tcaaaatgcc tgattcagta ccaccagcag tatctaaagc
    46321 atttgcagcc attttaccag cgacgatcgc tttatacgtc attgctatta tcaattatat
    46381 cgtaggtcgt gtagctgatg ggcaactgat cattgatttg gtgcaaaaat atattgcaga
    46441 gccattccta ggattatctc aagggttggg agcagtattg atcgtcacag tatttgttca
    46501 agtattctgg ttctttggta ttcatggacc aaatgtattg gcaccagtgc tagagggaat
    46561 ctggggacaa gcacaactaa tcaatattga tattttccaa aaaggttata acggaaaaac
    46621 tggtacacca gccgtgttag atgcaatcga agatggaaaa gcttatatgt gggtccgcgg
    46681 atcgttcgat gcttttgcat ggtttggcgg atcaggcgga acgatcgtct tactgatagc
    46741 tatcttgatt ttctctaaac gtgcagatta tctaacagta gctaaattat ccattgggcc
    46801 tggtatcttc aatatcaacg aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat
    46861 catgtttatc ccattcttga ttgctccagt agtagcaaca gcaattggat ggacagctac
    46921 ttatcttggc ctagtcgctc cggtatcgca gcaagtcaca tgggtagtac cgccgttctt
    46981 attgtcattc ttagcaacag gagcggattg gcgggcaccg atcgtaacat tagtatgtat
    47041 ggtcgtgact ttcttgattt gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc
    47101 cgcaggcgaa caacaataag atgaacgtga ggagcgggac gctttaagtt tcgttcctca
    47161 taatgaattt tcgaggagga actgaaatga ttacaattgc cagtgcaatg caacaagatg
    47221 aaatcaaaga attgttagaa ggatacaatg aagaaggaat gacttttaca tttaaagaaa
    47281 aacaaggaat caagctgttc tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa
    47341 aagcaaagga actaatcaaa gcacagcctt ggggaacagt cctttatttt caatcaacag
    47401 ctgtttagaa aatggtttaa aaagatagga tggttgagat gataaaaaac ggtttatttt
    47461 tatgtagtat tggttctttg atcgtgttgt atagtttgaa tccacattcg caaaggtatg
    47521 atttgtatag tatggtcaca ggtctttttc taacaggatt aggaatctat ttcttcatga
    47581 aggggaaaaa aagagaagaa gcaaaggaga aaaataacta atggaaatca cgaaaaaatt
    47641 aaatattcca gcatcgtttt tttacgataa agttatggac tctgtccttt tcgatgtacg
    47701 taaagcgacc ggcaaatcag taactagaaa acagctgaaa aatttggagt acgtcaaaca
    47761 attctcgcaa aataaccggg ctcgcataaa aatcgaagaa gtcattgaaa accagtctta
    47821 taagtttcgt acatcgacaa cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt
    47881 ggacgattcg acctgtgagg ttcattacac agaaaaaatg gagtcttatg gcatgcttca
    47941 aaagttgaat gatatgcttt taggaacaat cttgatgtat tttaaacgga gacgtttcaa
    48001 aaaaatgcta gatatgatcg aagcaacgta ttaaatcaat gaaacatggt actatttagt
    48061 tattgaaaaa tgaagtaagc tagaaggaat ttttcatagg aagatagatc tttcctgcga
    48121 atcctcacta gcttttttgt aggaaggggc agcaaaatga caaacaaagt aagagagcta
    48181 ggtgtatctt cgattgagga aatgtttgat ttagccactt atgcatttaa tggggcagat
    48241 acacctgaaa ggcgagaacg ttttcgtaca cttgcggaga attcttggaa ttacggattt
    48301 tttgatgagg aggatcggct aacaagtcaa gtcatggcga ccccttttcc agtgaatttt
    48361 tacggacaag agtacctaat ggccggcatt ggctatgttg cttcttatcc agaagcaagg
    48421 ggacaaggcg ggatcaatca gatcatggaa aagatccttg aagattgccg tgatcgtaaa
    48481 gtaagcttat cttatctggc gccattctcc tatcccttct atcggcgcta tggctatgaa
    48541 caatcatttg ataaaatcag ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa
    48601 aaatctggaa aaataaagcg tatggatttt gaagatgcaa aagctgcaat cagaagaatt
    48661 tatgaacaga tgccggaaaa ccaacgagct ggtctgaagc gcgaagactg gtggtatacg
    48721 tataaattca agcaaaaagg caattaccag tttgctttgc attttgatga taatgaggaa
    48781 gcaacgggat atatcgttta tcagcttgca acgtcttcct ttattattgc ggagtgggga
    48841 tttttggatc atgatgcttt ttgcagcctt gttcgcttcg taagttctca caatggtgct
    48901 tttgaaacat ttgaatattc atgcggctac ggcggtaatg atcaaaatta tctattagaa
    48961 aatccatttg cttctgtaat gatcacacct tatatgatgg cgaggatcgt agatatttct
    49021 ttgtttttgg aaaattatcc atttaaaaag agacaagctg catttgccct acaaatagaa
    49081 gaagatcatt atgcgaaatg gaatgaagga atcgtggaag taacaatcga aaatggaaaa
    49141 aatcatctct caaaagtaga acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat
    49201 atcacgcagc tattattagg gtatcgtaaa gtggaagagt tggtattcca agaaagaata
    49261 caggtgacaa aagaatatga agaaacgtta agctcaatct tgccacagca acagccaatt
    49321 cttaatgact atttttgata aaaaacgggt gatgacaata tttttgtcac cacccttttg
    49381 tttttcattt ggcatttgaa ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga
    49441 attttttgtt ttcttttgtt ttagggtttt cttttttgtg gtcttggatt gcttctttcg
    49501 taatcttttc ccgatttttt ttagatgaat ctaagcgttc tgcgtatact ttttcttttg
    49561 gcttatcatt cattggaaag gacctcccta cattatttca ttctacctct agtttatcat
    49621 ctgttaagga ctagtggaaa cgttttgcct aacttataag ctgatttttt tagcagataa
    49681 gcttgcaagt aaaaaacaaa caagctacaa ttgatttatt gaaattgttc ctgtagctca
    49741 gtaggataga gcaatcgcct cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg
    49801 tacaaataac ccctgtatcc tttgcggtac agggggttat tttgtataag tagacattgt
    49861 agtttctaaa ctgctatgtc ctaaacgttt cgatacgcta agtatattta ctccttgata
    49921 aagtaaaata gatgcatgcg tatgtcttag tccatcaata aacagtcgaa gagaaaatag
    49981 ggaaaaattg attactaaag aaaaacttac taggtcgacg gtgtagttga ttttcatact
    50041 tcaatagatt cgaattaaac gatttcgatt ggcttatacc agtcctatcc aacgaaagat
    50101 atatcgttct taactctagc ggtataactt gaagctttac gtaaagccaa tgggaatgaa
    50161 ctggagatga ctttttatta tttttagtaa tagctgatgg gttaatactt acaactttcc
    50221 ttttcaaagt accatataaa aaaacggtgg ggttgtttag cgaaataaag gatctaagcc
    50281 aatagcttag atcctttatt ctatgcttta tattattttt tgcatgtgtg cgaaagtata
    50341 ctcaagccca atcgccattt cggaaaatcg gtactgtaga gccatcttca cggattccgt
    50401 cgatatccat cttgtcagat cctaccatga aatctacgtg ggtttgactt ctattcaatc
    50461 cagcttctgc taattcttca tcggacattt ctgtaccgcc tttcacactg aaggcataag
    50521 cagaacctag tgctaagtga ttcgatgcgt tttcatcaaa taatgtattg aaaaagatga
    50581 tgccagattg agaaataggt gaagggtcag gtacaagtgc gacttctcct aaacgacgcg
    50641 caccttcatc tgtatcaagc agttttgcta agacatcttc tccttgttcg gcagaaaaat
    50701 caacaacttt tccatcttta aaagtaaatt tcatacccga gatgatggtg ccagcataac
    50761 tcagtggctt tgtacttgaa atgtaaccat ctactcgatg actgtcaggt gctgtgaaca
    50821 cttcttccgt cggcatattt gccatgaatt tttctccacg agcattatag cttccagcgc
    50881 cttcccaaag atggttttta ggcaatccga taatgatatc tgtaccggga gcagtatagt
    50941 gaagggcaga aaattgttct cgattcaatt cttctgcttt ttttgctaat ttttcatcat
    51001 gcttcttcca tgctaagaca gggtcttctt catagacacg agtcgttttg aaaatctgat
    51061 cccataatgc ctcaacttgt ttttcttctg gcagttctgg gaagactttt gctgcccatt
    51121 gtttaccagc cgcagccacg acagtccaac tgactttatt tgcttgagtt gccttgcgta
    51181 aattcattag tgctttgcca ttcgctgatt gataagacgc tactcgatgg ctatctacac
    51241 cagcaaatgc atcgggatcg gcagaaacaa cgctgatcct gcttgctcct ttttcaagcc
    51301 attcatccgc ttgatcgatt ttagattgtg gtacattctc aatacgatcc gttgcagcat
    51361 gtaaaagaaa ttctctttgg atctggtcgt cagtccattg aacgataacc tcagcggctc
    51421 ctagtttata agcttcttgt gtgatcaatc gagcaagggg tgcctgttcc acgctgatct
    51481 gtaaaacaac tgtgtgtcct ttttctg
//
LOCUS       DANKAZ010000009        50449 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000009 DANKAZ010000000
VERSION     DANKAZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..367
                     /locus_tag="PWY67_000482"
     CDS             <1..367
                     /locus_tag="PWY67_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDL2056791.1"
                     /translation="ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQ
                     NVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYV
                     TVSNDKDGVSQAFERILTI"
     gene            648..1868
                     /locus_tag="PWY67_000483"
     CDS             648..1868
                     /locus_tag="PWY67_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL2056792.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1865..4585
                     /locus_tag="PWY67_000484"
     CDS             1865..4585
                     /locus_tag="PWY67_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2056793.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4569..5513
                     /locus_tag="PWY67_000485"
     CDS             4569..5513
                     /locus_tag="PWY67_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2056794.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5582..6592)
                     /locus_tag="PWY67_000486"
     CDS             complement(5582..6592)
                     /locus_tag="PWY67_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2056795.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6772..7116)
                     /locus_tag="PWY67_000487"
     CDS             complement(6772..7116)
                     /locus_tag="PWY67_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2056796.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7109..7543)
                     /locus_tag="PWY67_000488"
     CDS             complement(7109..7543)
                     /locus_tag="PWY67_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL2056797.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7681..8418
                     /locus_tag="PWY67_000489"
     CDS             7681..8418
                     /locus_tag="PWY67_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2056798.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8411..9622
                     /locus_tag="PWY67_000490"
     CDS             8411..9622
                     /locus_tag="PWY67_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2056799.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9826..10608
                     /locus_tag="PWY67_000491"
     CDS             9826..10608
                     /locus_tag="PWY67_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL2056800.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10678..11340
                     /gene="trmB"
                     /locus_tag="PWY67_000492"
     CDS             10678..11340
                     /gene="trmB"
                     /locus_tag="PWY67_000492"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL2056801.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11438..13192)
                     /gene="ade"
                     /locus_tag="PWY67_000493"
     CDS             complement(11438..13192)
                     /gene="ade"
                     /locus_tag="PWY67_000493"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL2056802.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13324..14253
                     /gene="tadA"
                     /locus_tag="PWY67_000494"
     CDS             13324..14253
                     /gene="tadA"
                     /locus_tag="PWY67_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL2056803.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14210..15259
                     /locus_tag="PWY67_000495"
     CDS             14210..15259
                     /locus_tag="PWY67_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL2056804.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15256..15534
                     /locus_tag="PWY67_000496"
     CDS             15256..15534
                     /locus_tag="PWY67_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL2056805.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15509..15979
                     /locus_tag="PWY67_000497"
     CDS             15509..15979
                     /locus_tag="PWY67_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL2056806.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15996..16298
                     /locus_tag="PWY67_000498"
     CDS             15996..16298
                     /locus_tag="PWY67_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056807.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16315..16686
                     /locus_tag="PWY67_000499"
     CDS             16315..16686
                     /locus_tag="PWY67_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL2056808.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16658..17017
                     /locus_tag="PWY67_000500"
     CDS             16658..17017
                     /locus_tag="PWY67_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056809.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17189..18196
                     /locus_tag="PWY67_000501"
     CDS             17189..18196
                     /locus_tag="PWY67_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2056810.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18221..19405
                     /locus_tag="PWY67_000502"
     CDS             18221..19405
                     /locus_tag="PWY67_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL2056811.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19842..21425)
                     /locus_tag="PWY67_000503"
     CDS             complement(19842..21425)
                     /locus_tag="PWY67_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2056812.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22003..22146)
                     /locus_tag="PWY67_000504"
     CDS             complement(22003..22146)
                     /locus_tag="PWY67_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL2056813.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22368..22847)
                     /locus_tag="PWY67_000505"
     CDS             complement(22368..22847)
                     /locus_tag="PWY67_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2056814.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22920..23204)
                     /locus_tag="PWY67_000506"
     CDS             complement(22920..23204)
                     /locus_tag="PWY67_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056815.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23282..23476)
                     /gene="ssrS"
                     /locus_tag="PWY67_000507"
     ncRNA           complement(23282..23476)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PWY67_000507"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23548..24834)
                     /locus_tag="PWY67_000508"
     CDS             complement(23548..24834)
                     /locus_tag="PWY67_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL2056816.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24972..25643
                     /locus_tag="PWY67_000509"
     CDS             24972..25643
                     /locus_tag="PWY67_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL2056817.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25609..26097
                     /locus_tag="PWY67_000510"
     CDS             25609..26097
                     /locus_tag="PWY67_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL2056818.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26110..27057
                     /gene="prmA"
                     /locus_tag="PWY67_000511"
     CDS             26110..27057
                     /gene="prmA"
                     /locus_tag="PWY67_000511"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL2056819.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27058..27822
                     /locus_tag="PWY67_000512"
     CDS             27058..27822
                     /locus_tag="PWY67_000512"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL2056820.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28055..30268
                     /locus_tag="PWY67_000513"
     CDS             28055..30268
                     /locus_tag="PWY67_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL2056821.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30279..30725
                     /locus_tag="PWY67_000514"
     CDS             30279..30725
                     /locus_tag="PWY67_000514"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL2056822.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30896..32155
                     /locus_tag="PWY67_000515"
     CDS             30896..32155
                     /locus_tag="PWY67_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL2056823.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32244..33191)
                     /locus_tag="PWY67_000516"
     CDS             complement(32244..33191)
                     /locus_tag="PWY67_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2056824.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33585..34877
                     /locus_tag="PWY67_000517"
     CDS             33585..34877
                     /locus_tag="PWY67_000517"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL2056825.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34892..36661
                     /gene="aspS"
                     /locus_tag="PWY67_000518"
     CDS             34892..36661
                     /gene="aspS"
                     /locus_tag="PWY67_000518"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL2056826.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36770..37645
                     /locus_tag="PWY67_000519"
     CDS             36770..37645
                     /locus_tag="PWY67_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2056827.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37911..39794
                     /locus_tag="PWY67_000520"
     CDS             37911..39794
                     /locus_tag="PWY67_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2056828.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39869..40153)
                     /locus_tag="PWY67_000521"
     CDS             complement(39869..40153)
                     /locus_tag="PWY67_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2056829.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40143..40877)
                     /locus_tag="PWY67_000522"
     CDS             complement(40143..40877)
                     /locus_tag="PWY67_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL2056830.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41006..41629
                     /locus_tag="PWY67_000523"
     CDS             41006..41629
                     /locus_tag="PWY67_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL2056831.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41821..42942
                     /locus_tag="PWY67_000524"
     CDS             41821..42942
                     /locus_tag="PWY67_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL2056832.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42949..46077
                     /locus_tag="PWY67_000525"
     CDS             42949..46077
                     /locus_tag="PWY67_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL2056833.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46096..49290
                     /locus_tag="PWY67_000526"
     CDS             46096..49290
                     /locus_tag="PWY67_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL2056834.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49436..50143)
                     /locus_tag="PWY67_000527"
     CDS             complement(49436..50143)
                     /locus_tag="PWY67_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2056835.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16474 a         8858 c        10505 g        14612 t
ORIGIN      
        1 tatcagcatt tacaaatttt ttatttttat tcctaatcgt caattgaaag ctaatatatt
       61 taaccatttg aaaaagatgc taaatgtgtc tgttactgaa tcggcacctg taaatattga
      121 aatcgtacct caaaatgtat ctaaaggtgt ggtgtttgat cgtttggaat caatctacaa
      181 tttaaagaaa cctttgagaa tcgcgatcgg agatagttta aatgacttat ctatgtttga
      241 aagtgcagat attagttttg caatgggaaa tagtcatcaa gaaatcatga aaaaagctga
      301 ttatgtcact gtttcaaatg ataaagacgg agtatctcaa gcatttgaaa gaattcttac
      361 catataaact aattaaggta gtatagaaca tagaattcta aatacggaaa aatggagcat
      421 aaattccagt acaaatagta cttcttcagt gtcaaacgaa gcgaatataa catagatatt
      481 taggattgtg acaaaaatca tgtcacaatc tttttttcga ataaacttat tttttgaagt
      541 cggccattta tcgtaatttc tttgcataat acgaacaaat tttcggttat tgatgaaaaa
      601 aaacattgaa aatggtatga taaaagaaaa aaaggaggaa atttttaatg gtgaaattta
      661 tccatgcggc agatttacat atggatcggt catttgaagg actgacaact cttgataaaa
      721 ctgtacagga aaaactattg aagacgaatc tgaccgtttt atcaaatatc attgaacagg
      781 caataataaa taacgttgat tttgttttac ttgcaggaga taattttcat caaaatcgtc
      841 cttcattaaa aatacagaaa catttttcag aacagatgaa acggttagaa aagaaccata
      901 tacctgtatt tataatattc ggaaatcatg atttctatca aaaagagcgg tattggttct
      961 cctttcctaa aaatgtccat ttatttacat ccgaaacggt agagacaaaa aaaataacga
     1021 taaaaagtgg agaaactgtc tctctcagtg gatttagtta tcgtcaaccg tggattcaaa
     1081 aagacaaagt gatggaattt ccttctagag aactcacaga ttaccatatt gggttgtacc
     1141 acggggaacc tggcgtctct caaaaaggaa actatgctcc tttccaaccc tcaaagatgc
     1201 aagaaaaggg atacgattat tgggctttag ggcatatcca tgtgccaacg gtcttaaatg
     1261 aaaagcaaac aattgtttat ccaggtgcgc ctcaaggtca tactaaaaag gaacaagctt
     1321 ctacatctat tctactagtt gaattaagta aaggaagttg ccaaatacat ccgattaaag
     1381 tagcagaggt ctattggaag acaaaggaaa tatcattgag gaaagcaaga acgacaaaag
     1441 aggtcattgt tcacattcgt cagcagcttt caaaagtaga ggatggattt agtttaattg
     1501 agataaaatt aaaagattat gaccatctca atacagatgt tttggaacgg atacaatcag
     1561 gagagctatt agactatttg ttagaggaat tctcagatag gataaatgat ttctttattt
     1621 ggcgtatttc tctcatagag aatacttttt atacgaagac cccacttgct gcttctgcta
     1681 aattgatgga acagttattt caatactatc agacacctca agatttccaa caaattttaa
     1741 atgaagtata tagtcatcaa gaagcggcta gaatattgag cgaactgccg gaatatcaag
     1801 aagaaacact tgaaaaagcc aagcagctgc ttaatcaaga ttttttattt gaggaggatc
     1861 aggaatgaag attttaagga tcgagattgc tgcttttgga aaatggcgtc aaaaaagttt
     1921 tgacttttat tcaggcaatc agctgatcta tgggggaaac gaagcaggaa aatcaacgat
     1981 ttatcaattt attcaagcaa tattatttgg tttcccttca aaaggccgta aaaaaaagga
     2041 ttatacacca aaagacggct cagcctatgg cggcaaaata tggcttaaac atcctgtata
     2101 tggtgaattt gctgtcgagc gctacaagca gcaaaatcgc ggaaaatcga aggtgtggct
     2161 gggggatcaa gtgggaagtg atgagttact agaaaaactg atcgcacctt tgacgaaaga
     2221 gttattccag caggtattta ctttccagca agagcagttg atggaactag atcatttgag
     2281 agaaaaagag ctacatgatg cgctgatttc tttaggcatc acgggcagtt ctcaagtatt
     2341 ccaaaagcgg caagaatatt atcagaaagc acaacaatta ttcaagaaaa aaggccaaaa
     2401 attactaatc aatcaaaaat taaatgaatg gaaagagctg caagaaaaaa ttcatcaaaa
     2461 acaagaacag gaagcttatt ttggcaagtt gatccaacaa gaacaggaat acgagtcgca
     2521 acagcagcag ttattggcac aattaaaaga ggcgaacaaa caattttcat tagccagaca
     2581 gcaagaactt aatttttctt tatatgaaga atggcaatct ctcaaaaaaa acagcactac
     2641 aggtcttcct gatgagcaac ttcttttaga tctggagtct ttctccaaac ggtatcagca
     2701 aacaaatgag cgtttggaag aattagaagc aacactggaa aagcatagcg ggatggatca
     2761 gcagtcagcc agatattatt tttatttaga gcaggagcag gaactaaaag atctccagca
     2821 acttcaattt acagcggaac aattaaacga agaatggcaa agattacaaa ttgagactag
     2881 agaaacaaat tcagaattga agattttgga acaaaggtgg cattggcaga aaaatccgtt
     2941 accggaagaa tttttatatg aagaagaatg gaagcatctt ctggctgaag aaagaaggat
     3001 tgctgatcta gaaagccaga tttctgtgcg tctccaaata gctaaagaac agcaagagat
     3061 gattgaagca gaagtgaatg acttggaaga gacctatcca gcattattac aaaatcagtc
     3121 agagatagca ccagtctctt catctaaacc gtatatgctg atggctggcg gagcaataat
     3181 cattgcaggt atatttgtac caatgccctt gaaagtgatc cttttactaa ttggtgcagg
     3241 catgattggc tatttcttct acaaaaggca accaaaaatt aaaactaaaa atgatttctc
     3301 tgagataaaa ggaatgtggc aagaaaaact aggacaactt gatctagcct tagctcaatt
     3361 agctgaagca gaagaaaaac aaaagagaac tggagaagaa aaacagattc ttcagcaaga
     3421 agtatatcgt tttgcggatc atcggcactt aggaaagatg gatacgctca aactcatgaa
     3481 agaatatgga caggaagtca tagattatca gaaattatat ggcgtatttg agcggaaaca
     3541 taaaagagaa caggaaatac aagaaaaact acgacaaatc gaccaaaaat ttgagtttgt
     3601 taaagaatgg ttaccgatac aagataagac aatttcagaa aaaatggtat tgctttcccg
     3661 ttttatacaa gagatggaac agatgaaatt tgccagaagc tatcagcaaa atactttatt
     3721 gaagcaagaa atcaatcaat tgaaaaagaa acaaaaagta tggatacaag aatacagcga
     3781 acaattgact caagcaggct tatcttatcc ttcggaagta cctgtctttt tacaaaaagc
     3841 caaacaagcc gaacagcaga tgaaaagata caatgaatta tcggaaatgc ttactccact
     3901 atttcctgaa gagctgacaa aagagaaatt atctgtaaga ctgcgccaac tgaaagaaaa
     3961 gcaatcccaa ctccagcaag aagaaaaaga agcaagtgaa aatcggcaac gactccaact
     4021 tcaagtagaa gagttgcaaa gagacggcac attggacgaa ctttatcaac aagctagccg
     4081 gcaaaaagca aaattacagg aattactgga ggaatgggga gcacttgaaa tcgccgggac
     4141 attcttaggt gatctttcaa cagagatgag tgaaagacaa ttaccccagt tgttaagagc
     4201 agcaggtgat tatttaggta ttttaacaaa aggaaattat cagaagatat tactgaaaaa
     4261 tgatacactg actttaacag acggatatac agaatttccc atctatgagt tatctacagg
     4321 tacgaaagat cagctgatta tggcgatgag atttgcttac ctctcattag aagctaaccg
     4381 ttcgatatgc ccgatcatca ttgatgatgg ctggcttcat tatgatcatc agcgaaagta
     4441 tcaattagct gaacttttag ctaaatttgg agaaaagtat caaataattt gtttttcttc
     4501 tgataaagaa atggtaagct attaccatga gatgaaccaa atcgtaaaac aattagaagg
     4561 agcaaataat gaaaaaaatt cgtgatttag taatcgatga gcagtttgaa caatttgtcc
     4621 tgatcaaaag tgcagatgta agaatcgcta aaaacggaaa aaaatttatt gcttttactt
     4681 tccaagatac ttcaggtact atcgatggta aatactggga tgcttcggag gaggaaatca
     4741 ataaatttac agccggcaca gtcgtttttc taaacggtaa aagagaggtt tatcaaggaa
     4801 accctcaagt aaagattctt catatgcgct taacaagggt agatgagcca ggagagccta
     4861 gtttatacat ggaaagagcc cctttgaaga aagaagaaat ggaagaagaa atcaatcaat
     4921 tgttgtttga gatcaccaat gcgcattgga atcgtattgt tcgctatcta cttaataaat
     4981 atcaaaaaca attttttgag tttccggcag caaaaagaaa ccatcatgca tttgccagtg
     5041 gattagctta tcatactttg acaatgcttc acttagggaa atcaatcgcc gatgaatatc
     5101 ctgagctgaa tcgagcattg ttgtatgctg gtatcatttt gcatgattta ggaaaggtga
     5161 ttgaactttc cggtcctatg tcgacagaat atacgcttgc aggaaatttg atcggtcatc
     5221 tggtattgat tgacgaagaa ataactaaag cctgtcttgc gttgaaaatc agcgaagctg
     5281 acgaagatgt tcttgtatta cgtcatatgg ttttagcaca ccatggctta ttagaatatg
     5341 gctcacctgt acgtccaaga ataatggaag cagaaatatt acatcaaatc gacaatatcg
     5401 atgcttcgat gcagatgatg ttgacttcga ttcgtcaaac agaaccagga aaatacaccg
     5461 atcggatttt cggtatggac aaccgaagtt tctatgtacc aaaagattta tagcaaaaat
     5521 ttattttttt cattcaaaac aaaaaaagcg agacataagt cttcgctttt tttgttttct
     5581 tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa tcagttgatt
     5641 ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta cttgatgaag
     5701 ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt acatttgcat
     5761 cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt ttagtttcag
     5821 tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta ttttggtttt
     5881 tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt gtagtaataa
     5941 catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct gcaggaatag
     6001 ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct gtatcagttg
     6061 atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt ttcacatctt
     6121 ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga tggaaagatt
     6181 tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag ccagcttcaa
     6241 aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca gcagcttcta
     6301 attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct gcatccactt
     6361 gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg atcatccgtt
     6421 gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca gatacagtga
     6481 tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa gctgctagag
     6541 ttaaaacagc caacgcacta gtagctgcca aaataattga tttttttttc attaaaattt
     6601 gcactcctgt aattagttat tatttttata tattaactat accatatccc atttccttta
     6661 aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc ctaggaaata
     6721 cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt attatatttg
     6781 ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga tttgtgtgat
     6841 acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt cttggaaagc
     6901 aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa aatgattcag
     6961 gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga tcagtttctt
     7021 tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc cagcagcagc
     7081 tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc tttacgattg
     7141 tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg acaaagtgaa
     7201 tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag
     7261 attgataggc tagttcacgg ttgttattca agatattaag acctttcatt tctggaaaag
     7321 ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct gcaagttcgg
     7381 gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg tgtcctttcg
     7441 ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt ttatagcttg
     7501 ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac atcctttcta
     7561 taaagaataa atacttcagc ttaactttac cataagttaa agctattgta aggaaaaaaa
     7621 caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg aggaaaaaga
     7681 atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt gttaaaagat
     7741 atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa cggagcaggg
     7801 aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg aacgatagaa
     7861 atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt
     7921 ccagagactc cttctttata tgaagaattg acattgagag aacatatcga agtcactgcg
     7981 atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt gttggagact
     8041 tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg aatgaaacag
     8101 aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat cgacgaacca
     8161 tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt agaaatgaaa
     8221 aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga aaaatattgt
     8281 gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct tgagatgttg
     8341 agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc tttaacaaag
     8401 gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa agaaaatgat
     8461 gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt tcttgattgg
     8521 cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg
     8581 agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga tggctacttt
     8641 tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc aaacctattt
     8701 ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat tggcatgcgg
     8761 attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta
     8821 tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag cagaattgtt
     8881 tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt cttctctgat
     8941 ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg cattagtaca
     9001 ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg attgggagaa
     9061 aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca atttattcac
     9121 agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc ctatattgac
     9181 tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa gattggcaag
     9241 aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt tattgattgc
     9301 cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat atttgatcgg
     9361 tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc agctttatcc
     9421 aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg tattaattat
     9481 cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc agcgatgggt
     9541 tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg taccaactcg
     9601 tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat
     9661 agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc tggttttttg
     9721 aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc aggcgactag
     9781 aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat ggagtttcag
     9841 ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac atacataggc
     9901 atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt ggctgctctt
     9961 tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa tggcgatact
    10021 ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga
    10081 aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa aaccatgctg
    10141 acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa
    10201 ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag
    10261 cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt aaaccatcgt
    10321 aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat catgtttgca
    10381 gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc tagctggaat
    10441 cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa atttcattgg
    10501 tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga agatcatcga
    10561 cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg tttggcccaa
    10621 actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag ataaataatg
    10681 cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt
    10741 agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc
    10801 cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa agcccatcct
    10861 gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc actagacaag
    10921 ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc agcgttgaca
    10981 gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga tccatggcca
    11041 aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt agatgaacag
    11101 attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt gtttgagtat
    11161 tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt agatttacat
    11221 caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc ttcaaaaggt
    11281 caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa aagtctgtga
    11341 aactaacttt ctgctttcat aaaaacatta atatccataa ataaatctca gaaaatgaac
    11401 attacagatg taatgtattt tctgggattt atttgattta aaatgtattt atttcatcgg
    11461 tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct ttgtcggtga
    11521 tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg
    11581 ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt tcatagacga
    11641 tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa gctatttcct
    11701 gctgtttgtt gacaacggtc atcccgccac ctttttgaat caaaagatta gcagcataca
    11761 gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg
    11821 tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca atgtttcccg
    11881 tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt gaaggaatga
    11941 atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga ctattcggga
    12001 taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa tgtggattta
    12061 atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga tcagcaaata
    12121 aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa acttcatgaa
    12181 ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca ggggcaatcg
    12241 cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc tgaatggctt
    12301 gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt gatccttctt
    12361 ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt gaattttcag
    12421 gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct cgaaccatta
    12481 aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct tcatgatccg
    12541 tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca gctgcatgcc
    12601 cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg tctgtttcat
    12661 tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg ggtttttgta
    12721 aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag
    12781 gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca tcttctatca
    12841 taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct gtcactacag
    12901 tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa agtgagcttt
    12961 cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta gcattatgta
    13021 cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt gctacatctt
    13081 tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc actaggtcag
    13141 caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc aatggagtgc
    13201 ctcccaagat aaatttttat aagtataaca taaattcggc ttttgccaat atttttttgt
    13261 aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt ggaggggaga
    13321 ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc caaacatgcc
    13381 caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg ctatggaaaa
    13441 aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat ctcaagattt
    13501 aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg tgcatttact
    13561 tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa
    13621 cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca tcgttgcttt
    13681 agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt ataccttttc
    13741 agcgggacag tcggatcagg caagacttct ctaatgtatc ggttggcttt ggaagaaaaa
    13801 ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt tctgcagcta
    13861 cagatcaatc cgaaaatagc acagacttat gatacacttt tgaaactcgc attacggcat
    13921 cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca tgctgcggta
    13981 agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt
    14041 acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga atgtttagct
    14101 ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt tttatggagt
    14161 tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta tgaagaagct
    14221 caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc agcaaaacca
    14281 gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag aatcccttta
    14341 tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc aacatcagct
    14401 aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag cagagcaact
    14461 ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt tggaaattgc
    14521 caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc ttgcatatcc
    14581 gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt tcgttttgcc
    14641 tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc tgatacaagt
    14701 ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt tactagtaag
    14761 gtatcgtttg atgaagttgt ctcctgttga tcagagtgaa tggataagca gacaattatt
    14821 gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga ttggaaagct
    14881 ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga
    14941 aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag gtatgtcatt
    15001 ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa ttgttttaca
    15061 gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat ggacaagaaa
    15121 acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca tttttttggg
    15181 cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt acggaagcat
    15241 agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt tacattgatc
    15301 gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt ccctaatttg
    15361 tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca gttggtagac
    15421 acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt ggaggaattg
    15481 ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc ttgaaggtaa
    15541 ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat tacttacaac
    15601 gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg cagtctatca
    15661 atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca
    15721 gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc atgttccgaa
    15781 tccagataaa ataaattggg aagtattaga tattcctgat gagattacct tgaaacgcca
    15841 tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt
    15901 ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga gtggtcgcta
    15961 tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca gcgcttatga
    16021 tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt caggcagaaa
    16081 agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc aagacgcagc
    16141 ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat atccaaattg
    16201 tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta
    16261 ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg actgatgata
    16321 ttgagcagtc tattgctcac attcaatctt ttgattcagc aaaccatcca agtaagtgaa
    16381 tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt agaagaagaa
    16441 ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac gatcgaggat
    16501 aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac ttccggcgga
    16561 catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga acacacaatt
    16621 tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac aaaaaacgat
    16681 cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat ttcttttatg
    16741 tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc ttaatatacg
    16801 aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg aacagtctac
    16861 gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa catatcagtg
    16921 ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc ggttcacaga
    16981 agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg aaaacgccct
    17041 cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac
    17101 agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt
    17161 tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac aagggtttaa
    17221 tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct ttttagaagc
    17281 ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg tggatggagt
    17341 gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga agaaactttc
    17401 tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg gcagccagac
    17461 agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt tatttgtttt
    17521 tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg atattgcggc
    17581 aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg ggtaccaaac
    17641 agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt ctacaagtga
    17701 tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat tattaataga
    17761 tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg acgaaaaagc
    17821 aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt tattgctaga
    17881 gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc cgagtggatt
    17941 tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag gttatcttca
    18001 aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt
    18061 gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact
    18121 tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta aaaactggaa
    18181 atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa caattgcaat
    18241 caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag aaaccgtagt
    18301 tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca tcaagtacgg
    18361 cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat
    18421 gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa tcaccggagt
    18481 agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga tcgatgagaa
    18541 agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc ctgcaaactt
    18601 aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg ctgtattcga
    18661 tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac cggtgtctta
    18721 ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc gttatgtgtc
    18781 ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca
    18841 cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg atacttctat
    18901 gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata tcgatccagc
    18961 tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga tcaatgtttt
    19021 gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc gtgatcttga
    19081 aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg accgcatccg
    19141 aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg tctttactgc
    19201 tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt
    19261 tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag atatctctac
    19321 aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa tgattgcccg
    19381 tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt aggataaaag atgaaacgca
    19441 tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta
    19501 acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt
    19561 caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca atattttatt
    19621 ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc
    19681 ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca
    19741 ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag
    19801 cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg
    19861 agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc
    19921 aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct
    19981 tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg
    20041 ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa
    20101 tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga
    20161 gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg
    20221 gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg
    20281 ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca
    20341 ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat
    20401 agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat
    20461 tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt
    20521 gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct
    20581 ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt
    20641 tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa
    20701 cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg
    20761 gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc
    20821 tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg
    20881 agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg
    20941 cctccgctaa gttctgttaa ccgtgcttcc tgacagtgtg gaatccctaa gccttgcaag
    21001 atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc
    21061 tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg
    21121 tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg
    21181 ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt
    21241 ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt
    21301 gatttgccag ttccgttgat atcgactaag gcaatttttt caccagcttc aatttgacag
    21361 gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg
    21421 atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccctg gcctgtgtta
    21481 tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt
    21541 caactgtttt ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa
    21601 atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag
    21661 tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca
    21721 actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag agcttttttt
    21781 atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt
    21841 caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt
    21901 tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc
    21961 aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt
    22021 accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc
    22081 gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac
    22141 ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg
    22201 cgactaatcg ccctggtgtt cacttttttt attttacttg aactcattga ctgttttccc
    22261 tttattttaa acattataaa gaccgatgag tgcttgccgc gtcttttgtt tttatcacgg
    22321 ttttttgtgc aaaaaaacct gccgatagtt ggcaggtcgt ctacgtgtta agctttggtg
    22381 tggtctttag ctggcaattg gttttctaaa tcagttcgga caacaagcac atcgcaagct
    22441 gcgtttctaa taacatattc agaaacagaa ccaataaata aacgttctac agcattcaat
    22501 cctgtggctc ctaacataat caaatctact tgattatctt ctgggatttc tctggcaata
    22561 atttgttttg gtgagccata ttctacgaca gaggttacat tattcaagcc agctttcttc
    22621 gcattgcttt cataatctgc caatgtttgc tttgccattt cagtagcttg ttctgcaagc
    22681 attccatcaa aggatgaaac ggtttggaaa gaacgtgtgt caatgacatg tgctaataga
    22741 agttctccgt tatttcggat agctacattg accgcctttt taaaagctaa ttcagcttca
    22801 tcagaaccat ctacggcaac catgattttt ttgtattgtt gtaacataag cattcactcc
    22861 tcaagtcaat cgtattgtac tcttttattg tagaacactt tagggaaaaa gagaattatt
    22921 tactgctttg gttcagcaat tttttttgca aatcggatac tgaaggtggc agaaacaata
    22981 attacgatca gtaaaaagac caaggcaata aagcgttgat tgaaaaaagc tgttaaaata
    23041 cccattacgc ctaaaacagc atatgcagca ccgtaaaatt gtaggaatcc ttgattttta
    23101 tcatttttgt tgatcaatac aaaaaagata tcctgtttat taaataaata atagctaacg
    23161 aataataata ctaaagtggc gataatcatt aaaatcctaa tcatgaacaa atcctccaaa
    23221 ataagcaaca aatagaaaaa gcaatttgcc aagagcaaat tgctttattc tttaaaagat
    23281 ggtaatctcc gttaatgccg ttgtttgtcc tgtagtattg atcccgcgaa ttcaagcagg
    23341 tgggtcccat ggtcgtagct tcgaactacc aaatgataag gcatgttacc agcagtgaca
    23401 caagacgggt cccaagatat caataaaaat gttcggtcaa cacacaaaga ggacaattcg
    23461 aacatcacag atttcccacc tctatagtag catatgaatt taaaaagagc aatgagagag
    23521 ctcgcagaaa cagaaccgtt ttcttgtcta gttttttgga ggatgctttt tccactcttt
    23581 tatacggtaa tactgttgac ccagagcctg ttcatatttc cctgtatctt ttggctgata
    23641 atactgagca ttcttcagtt tatctggtaa atattgctga ttgacccaag cctgatcaaa
    23701 atgatgagga tactgataac ccacgccacg gttgagtgat ttcgcacctt tgtaatggct
    23761 atctctcaaa tgatcaggaa catctccagc tttgccctct cgaatatcag caagtgctgc
    23821 atctaatgcc atgtaggcag aattggattt tggcgacaag caaagatcga cgacgacgtc
    23881 tgctagtggg atacgtgcct caggcaatcc caatttttca gcagctaata cagcgttcac
    23941 tgtacgagcc gcagcagcag gattacctaa acctatatct tcataaccga taaccatcag
    24001 acgccggcaa atacttgcca gatcacctgc ctcaactaaa cgggcgagat aatgaagtgc
    24061 agcatccaca tcacttccac gaatcgattt ttgaaaagcc gaaataacgt cataatgagc
    24121 atctccattt ttgtcatggg taagagcttt tctttggaca cattcttcaa tgatcgacag
    24181 tgtaagatga atccttcctt ctttatctga aggagtcgat agtgtagcca gttccagccc
    24241 attcaatgca ctccgcaaat cgccgttcgt tgcacgcgaa aggtgaagaa gagcatcttc
    24301 gtctaactgg attgcttgtt gtccaaggcc gcgttcttta tcccttaaag catgttcgac
    24361 tgccaactgg atgtcttgtt ctgttaatgg tttgacttca aagatctgtg tacggctgcg
    24421 gatcgctgga ttgatggtaa tataaggatt ctcagtagtc gctccgatca agatgattcg
    24481 gccactttct aaatgaggaa gcagaaaatc ctgttttgtt ttatctaatc ggtgtacttc
    24541 atccaataag aggatcactg taccactcat ttttgcttct tccgctacaa cttgtagatc
    24601 cttttttgaa tctgtagcgg cgtttagcat gcgaaatgca taattagtag agccggcgat
    24661 cgcacttgcg atactcgttt ttccagtacc aggaggtcca tatagaatca tggaagagag
    24721 catgcgagca tcgaccatgc ggcgtatgat ttttccttcc ccgactagat gctgctgacc
    24781 aacgacttca tcgatcgttc gaggtctcat gcgataagcc aatggctgtt gcataggatt
    24841 ttcctccttc ttgtttttac gtcccttaat tatatcatat ttagcaagat gcctagaaag
    24901 aacagccttc tgttgttttg ttttcatagc ttatccaact aatattgtgt atgataaagt
    24961 aggtgagaaa aatgacggat aaacaaaaga tagatgaatt tttttggaat aaaacgacgc
    25021 cagaagtggc acgggattta ttggggatgt atttggagca tgacacacca gaaggaagat
    25081 tagctggata tattgtagac gcggaagcat acctaggtcc agaagacgag gccgcccata
    25141 gctatggttt aagaagaaca ccgcgtgtgc gggcaatgta tgaaaaacca ggtacgatct
    25201 acctttacac gatgcatact catcggattt tgaacatcat tactcagcca gaagggattc
    25261 ctcaaggagt aatgatacgt gcaatcgaac ccgccgcaat gattgatcaa atgagtaaaa
    25321 atcgtggcgg aaaaactggt ccagatatca gcaacggtcc gggaaaatta gtggaagcat
    25381 tagcaattcc tcaagaactt tatggtcagt ctatagcaga tagttcgtta cgcttagttt
    25441 ttgagaaaaa aaagacacca aaaaagatca atgctttgcc tagaatcggt atcccaaaca
    25501 aaggtgtatg gacagaaaag ccgttgcgtt ttgttgtgtc gggaaatcct tatatctctt
    25561 tacaaagaaa aaaccaaatc gagaaagatt ggggttggag gaaagaaaat gaaaaagaag
    25621 gatctattaa catatttaga tgaaataatt gaaaaacaag tgacagacta tgatgtagct
    25681 ttagattggg atacaaaaaa tcatacgatc gagatcgttg ttcgcttgtt tgcagaaaat
    25741 aaagcacaat ttgagattga tgatgcagaa gggatcgttt cgcaagaacc aattatcgaa
    25801 tttgaagacg gcgtacttct ttttaatcct cagaaatctg tttttgatga acgcaattat
    25861 ctagcagtaa tcccttatga aggaaaaaaa gggttggcaa aatcagtagc tgattcttta
    25921 gtggaatatc taaatgaagt attagctcaa gggcaaagcg atttgcttga ttttttggat
    25981 gaagacgatg acgaagctgt attcgagctt cactgggata atcaacaact agcagaatta
    26041 gtagaagtaa aacagcaaaa tgaagcagat acttatttac cgtacccaag ttattaagga
    26101 gtgacagata tgaaatggaa tgaagtaaaa gtagaaactg aaagtgaagc agtcgaagca
    26161 gttgctaata ttttgatgga ggcaggggcg agtggtgtcg cgattgaaga ttctttagat
    26221 gttgaacatt ttcagtcgga tcaatatggt gagattttag ataaaaatga ttttacatcg
    26281 ctgaaagaag gcgcactagt catggcttat tttccggaaa cgactttctt accggaaatc
    26341 ttgcctttta tcaaagatcg tatcacacaa ttacctgagt ttggtctagc tattggaaaa
    26401 aataaagtaa cagttagtga agtagaggaa agcaactggg caacagcctg gaagaaatac
    26461 tatcatccgg tgcgtattac gcgaatgctg acggttgcgc caagctggga aaactaccag
    26521 acagaagatc ctctagaaaa aattatcaca ctagatcctg gtatggcatt tggcacaggc
    26581 actcatccca cgacttcctt gacattgaca gcactagaaa cagtcttgcg tggtggtgaa
    26641 acattgctag atgtgggaac tgggtcgggc gtgctaagta ttgcagcaaa atatcttggt
    26701 gcaaaagatg tctatgcctt tgatttagac gaagtagccg ttcgttcggc gaaagaaaat
    26761 atggatatga atgaaatagc caaagatgtc catgtatcag ctaacgatct tttgacagga
    26821 atcgagattg aaagtgatgt aatcgttgcg aacattttag cagatatcat cttattgatg
    26881 atcccagatg cttggcgttt actgaagcaa acaggcacgc tgatcgtttc aggaatcatt
    26941 gaagagaaga agcaaatggt tctggatgcc atgttagaac aagggtttat cgtggatcaa
    27001 attttccagc aaaaagactg gtatgcaatt attttgaaaa aaccagaggt ggaatagatg
    27061 cagcggtatt ttttaaaaga atcatatgaa tcaaaagact attttgtttt agaaggggaa
    27121 aactatcacc acattgtacg ggttatgagg atggaacccg aggatgaagt ttttttggca
    27181 ttcaatgatc aaatagccat tgttgcagaa atcacggaga ttactgaaga ggctgtttat
    27241 ctgaaagaaa ttaaaaaaga agaaaatcaa aaggaactgc caagttcgat taccatagcg
    27301 agtggctatc ctaaagggga aaagctggat tggatcgttc agaaatccac tgaattagga
    27361 gcgcaagctt ttcttggttt cccggcatca gcctctgtcg ttaaatggga tgcaaaaaaa
    27421 cgtatgaaac gacaacaacg gcttgaaaag attgctcaag aggcagcgga acaatcccat
    27481 cggcagatcg tgccatcagt agagttattg gaaacgaaac aagatttatt tgagcggcta
    27541 gatgattatg acgtggtctt ggttgcttat gaggaatcag caaagcaagg cgaaaaatca
    27601 aatgcagcca aaattcttag tgaaacaaaa ccagatagtc gaatcttagc tgtctttgga
    27661 ccagaaggtg gattatcccg cgatgaagtc gattattttg tagaacacaa agggatactt
    27721 tgtggattag gaccaagaat ccttcgtaca gaaacagcac ctctttattt attaagtgct
    27781 gctagctatc aatgggaatt aatgaatcct tctttcagct aacgttcttc attaataaaa
    27841 gagtatcgac agaaagaatt gaaaaaatag tcaacaattt gtataataga aacaattatt
    27901 tatacaatgg atacgaaaaa tcgtttgaaa aagcttttaa aaagcgaaca tttccgtaaa
    27961 aaaacgtata actgcacgga ttttgggaca attatcaaac agatatttgt cccgattccc
    28021 gttttaccga aaaaagaaag ggggatcgat tcctgtgaca aaagaagaaa ttttaacggg
    28081 acccggtgtc ataaagctcg ttagtcaata catgggtccc gaacatgtag catttgttga
    28141 gaaagcatgc gaatacgcga ctgctgcaca tgacggacag tttagaaaat caggcgaacc
    28201 ctacattatt catcctatcc aagtcgcagg tatattagca gatttaaaaa tggatcccca
    28261 tacagtggct acaggcttct tacatgatgt tgttgaagat acagaaatca ctttagaaga
    28321 tctgagagaa gaatttggcg atgacgttgc tatgttagtg gacggcgtaa ccaaattagg
    28381 gaaaataaaa tataaatccc acgaagagca gctagcagaa aaccaccgaa agatgctgct
    28441 agcaatggct caagatttac gagttatcat ggtcaaatta gctgatagac tacataacat
    28501 gcgtacgttg aagcacctgc gagaagataa gcaaagaagg atcgctcagg aaactttaga
    28561 aatctatgcg cctcttgcac atcgtctagg gatcagccgg atcaaatggg aattagaaga
    28621 tacagcactt cgttatctaa atccaaaaca gtattaccgt atcgtccact tgatgcagac
    28681 gaagagagaa gaacgtgaaa aatacgtaag tggtactgtt gaagatattc gaatagcgac
    28741 agaagagctg gggatttttg cagaaatcta tggacggcca aaacacattt attcaatcta
    28801 tcgtaaaatg aaagatcaga aaaagcagtt caacgaaatt tatgacttgc tagctattcg
    28861 agtgatcgta gattcaatca aggattgtta tgctgtatta ggagcaatcc acacaaaatg
    28921 gaaaccaatg cccggcagat ttaaggatta tattgccatg ccaaaagcta atatgtacca
    28981 atctttgcat actaccgtga tcggtccggc aggaaatccg gtagaaattc aaatccgaac
    29041 acaggaaatg catgaaatcg ctgaattcgg ggttgctgca cactgggcct ataaagaagg
    29101 aaaaaatgaa aaagtagaac cagatggtat gacgaaacaa ttaagctggt tccatgagat
    29161 actcgaactt caagacgaaa gctatgatgc ttctgaattt atggaaggcg taaaaggaga
    29221 tatctttagt gataaagtct acgtcttcac accaaaagga gacgttactg agttaccaaa
    29281 aggatccgga ccattggact ttgcatacag tatccataca gatatcggta acaaaaccac
    29341 tggtgcaaaa gtaaatggca aaatggtgca gcttgattac aaattgaaaa acggagatat
    29401 cattgagatc atgacttctc caaattcatt tggcccaagt cgcgactggt tgaaattagt
    29461 tgctactagc aaagcaagaa ataagatcaa acgtttcttc aaagcccaag atcgagaaga
    29521 aaatgtgatc aaaggccacg aatccgtggt caaatgtatt acagatctag gatttacgcc
    29581 taaagatatt ttgacgaaga acaaactgca agaagcactc gatcgtttta attatcaaac
    29641 agaagatgat ctctatgcag ctgtagggta tggagaagtt agccccttga cgatggccaa
    29701 tcgtctgact gaaaaagaac gtaaagaaca aaaaatcgag cagcaaaagc aagaagcaga
    29761 agaaatcatg aatcagccga aaaaagaacc tgacaaaatg aaagtacgtc atgaaggtgg
    29821 cgttgtcatt caaggggtag aaaacttact aattcgtatc agtcgctgct gtaatccgat
    29881 tcctggtgat gatatcgttg gttatatcac taaaggcaga gggatatcca ttcatcgtcg
    29941 agattgtccg aatgttcagc ctgacaaacc aaatgtagca gaacgtttga ttgaagtcga
    30001 atgggaagat acatcgaata cacgaaaaga gtatgatgca gatttggaaa tttacggcta
    30061 taatcgttca ggcttattga atgatgtact tcaaacagtc aatgcgctaa cgaaaaatct
    30121 caacagcgtt gaagcacgga cgaataaaga taaaatggcg acgatccatt tgacggttgg
    30181 tatccagaat ttatcccacc taaagagtat cgtggataaa atcaaagcag tacctgatgt
    30241 ctacagtgta cgccggacga atggatagga ggacagatat gagagcagtt gttcaacggg
    30301 taagtaaagc tgctgtgtca gtcgatcagc aagaaattgg aaaaattggc tacggcctac
    30361 ttgttttatt aggcattcat gaaaccgata cgcaaaaaga tgtggactat ctggtaaaaa
    30421 aaattgcaca aatgcgtatt tttgaagatg aacaaggaaa aatgaatatt agtgtagaag
    30481 atgtgggagg acaaatttta agtatctctc aattcacact gtttgcggat acgaaaaaag
    30541 gaaatcgacc aagctttgtg gaagccgcac gaccagaaac agctattcct ttatacgaat
    30601 cattcaatga aggaatcaga agtcgcggca tcacagtcgc taccggacaa ttcggagcag
    30661 atatgtcggt ttctctcgtc aatgatggac ctgtaacgat cattattgat tctaaaaaca
    30721 aataaagtaa tctggttttc actagtaaaa actatgaaac acgtcgctta agttagactg
    30781 ataaactcta acttaagtgg cttttttgtc tgcaaaaagc gcttcttttg tcagcagtcg
    30841 gaaaaaaagt taagcttaaa ttatacaagt tattttttat aggaggttgc tgaagatgaa
    30901 agaagtacca gtagtagata tcaaaatcac agatttaaaa aaattgtatc aaaaaggata
    30961 caacatgttg gaggagttac gccatgaagc ggacgctccc gtcgtcaaag caaagatttt
    31021 caataaagaa gctatcacga tctacggatc atcagcagca aaagtatttt atgatccaag
    31081 gaatttcaaa cgaaaaggtg ctatgccaaa attagtattg aagacacttt ttggtcaagg
    31141 tggagtacag actttggatg gagctgctca tcaccatcgg aaaaatattt ttatggatct
    31201 aatgacaccg gaaagaatgg aagattatca tcgtatctta gataagaatc tgacacaagc
    31261 acttgaagca cagcatggtc aatttgaact atttgattta agtaagatgg ttttctttac
    31321 atctatttgc gagtgggcag gtatcaattt atcagcgatt tctaaagatg aggtcgaaaa
    31381 attagcagaa tatcaaatct ctatgatcag cggaacgttt acttctccga ttgatcacat
    31441 aaaaggtgtc gagaatcgca aaaaatcaga aaaatgggcg caaggcttga ttgaagaagc
    31501 tcgacaaaat cctgtggctg gaaaagaaaa tgtggcatta tatgcttttg ccaatgccac
    31561 agatttagat ggtcagcttt tacctctgga agtggcagct gttgaattat tgaacatcat
    31621 ccgtccaacg gttgctctaa ctgtgtgggc ggcattgatg ggacatgcac ttttcagtag
    31681 accagatttg tatcagcaat taaaaaatga cttttcgacg ttacaagatc cgtttattca
    31741 agaaatgaga cgctattatc ctttcttccc aatgctacct gcgatttcgc tgaaagaagt
    31801 agaagtagat ggttatcgta taccagaagg cagttgggtt attttagatt tatatggaac
    31861 agaccatgat gaacgaacag ttgaagcgcc agattccttt atgatcaaac ggtatgtagg
    31921 gaaagcaaaa gatatttcct ataaagaaga atatgagatg attgcacaag gcgggggaaa
    31981 ttttcgacaa atgcatcgct gtgcaggaga atggatcact cttcatagtc tacgtgtgtt
    32041 ttccgatcag ctagtaaata agtttgagtt ctctgtacct gaacaagatt ggacgattcc
    32101 attcaatcag tttccgactt atccgaatag ccgagcgcta ctttacaaga attagattag
    32161 cataaactta tcgaaaaaaa ggaagatcaa acagtgctga tcttcctttt ttcatattac
    32221 tataaattga taaggtcaat tgatcaaaat ccacgaaatg cttcatcaat ggtttgataa
    32281 tcttcattgg ataaatgcaa atctaaagct tggacattgt cagatacttg ctctggacgc
    32341 ttagctccag gtataactac agacactcgt ggattcttca tgtaccaagc taaaacaagt
    32401 tgcgcaggag tggcttggta tcttttagca atggggcgaa gtacatcgac agcggttaaa
    32461 atttctctga aacgttctcc ttggaaatct ggatttcttt ttcgaggatc tccttcacca
    32521 aattgttttt cctcaccaag ttcatatttt cctgtcagca aaccggaagc aagaggaaaa
    32581 tagggaacaa aactgatttt gtttttttca agataaggga ataattcttt ttcagcctgc
    32641 cgatggatca aactgtattt atcttctact acatcgacat atccatctgc attcgcttct
    32701 ttcaattgtt ctaacgtgaa gtttgacaca ccgactgctc ggatttttcc tgcttctttt
    32761 aattcgtgca atgtagcaac tgattcattt tttggtgtgc tttcatccgg aaagtggata
    32821 taaaaaatgt cgatgtaatc agtttgcaga cgttttagtg catcttccac cgattgtttg
    32881 agaaactcag gagaattatc aaatgttcgc cctttatttg gtacatgagc agcttttgta
    32941 gcgatgacga cacgtgagcg atcatattct ttttccttca gtacctcgcc aatcagttct
    33001 tcggaacggc cgtttccgta agcataagcg gtatcgatca aattgatccc gctattcaaa
    33061 gctgtacgaa ctacttgttt tcctgtctca tcttccaaac cgtcaaaaag attatgtcct
    33121 ccaacggcat ttgctcctaa tccaagctgt tctgcgtaga cttgtgaatg tccgattctt
    33181 atttctgcca tagctgattc ctcctttttg tctagtctaa aacaaaatac caaaaagctg
    33241 aactaaaacg tttttgtgag gggaaatagt cagatatctc ttgactttga gactggtttt
    33301 tagtaggtta taagtaatat cgaatgtcaa tgaaagagcg agtaagtttt tgaaacttta
    33361 caagagagga tcatcgaggc tgaaaatgat ctaaagtttc attagcctaa agacactctg
    33421 gagatggacg agtgaaaagc cgtcccgcgt acgagcgtta ttcgtaagag gaaagagaaa
    33481 tctttcaaaa ttaggtggta ccgtgtatga atttcattca cccttgttca tgatgaacaa
    33541 gggtgatttt tttatttaca tagctaagaa agggagagaa aactatgagt ttccaaagac
    33601 caaaaggaac aaatgatatt ttgccaggcg aatcagaaaa atggcagttt gtcgaagaaa
    33661 cagcacgttt attattcaga gattatcagt ataatgaaat gcgtacaccg atttttgaac
    33721 actatgaagt gatatcccga agtgtagggg atacaacgga tatcgtttca aaagagatgt
    33781 atgattttta tgacaaaggg gaacgccatg tgacgcttcg accagaaggt acagcaccaa
    33841 tcgttcgttc ttttgttgag cataaattgt tcggcccaga gtatgcgaaa ccgttcaaaa
    33901 ctttttatat gggaccaatg tttcgctacg aacgccctca aaaaggacgg ttacgtcaat
    33961 tccatcaaat cggagtagaa gcatttggat cagaaaaccc tgcaactgat gtggagagca
    34021 tggtgatggc acttgatttc ttcaaacagt tggggatcac gcaaatccgg ctagtcatca
    34081 attcattagg tgataaagaa acgcgtcaag cctatcgtca agcattgatc gactatcttt
    34141 taccttttga atcagagctg agtgaagact ctaaacgccg ccttcatgaa aatccattac
    34201 gcgtattgga cagtaaagac aaacgagatc aaaaatttgt ggcagaagct ccatctatct
    34261 tagattatct aagcgagcct gctcaaaaac attttgatac cgttgtttcc atgctggatg
    34321 cattggctgt tccttatgaa atcgacagta atatggttcg cggactagat tattacacac
    34381 atacgatttt tgaaatcatg agtgacgcac caaaaatggg ggcacaagca acgatttgcg
    34441 caggcggacg ttatgacaat ctagtggaag aactaggtgg tccttcaaca cctggcttcg
    34501 gatttgcaat gggtatcgag cgattactct tgactatgga ggcagaggaa gtagttatcc
    34561 ctgcatttaa tgagttggat gcttacgtgg tcgcactcgg agacgaaaca aatatcgaag
    34621 cattgaaagt ggtccaagcg attcgaaact ttggtttttc agctgaccgc gacttcatga
    34681 accgtaaagc aaaagcgcaa tttaagacag cagataaagc gaatgccaaa ttagtattgg
    34741 tgatcggtga agatgagctt gctagtcaaa cgattaatgt caaagcaatg gcaagccgaa
    34801 aagaaaaaag ttttccatta gaagagatat atgaacattt tgatgaagtc tatgatgaaa
    34861 tgactttatt cgagtaggag gaaaaaaata aatggcacaa agaacgatct attgcggatt
    34921 agtttccgaa caacaattag aacaaacagt tacattaaaa ggatgggtac aaaaaagacg
    34981 agaccttggg ggagtaatct ttatcgacct acgtgatcgt gaaggaatcg ttcaaatcgt
    35041 ctttaatcct gaaatcaaca aagaagcatg ggaaatcgcg gataaatgtc gcagtgaata
    35101 cgtgatcgaa gtgactgggg aagttagaag acgcggagaa aatgcagtaa atcctaaaat
    35161 ggccactggt gaattcgaag tgattgcaag tgaaatcgtg attttgaatc aagcaaaaac
    35221 cccgccattt caaattgaag atgatcaagc aatcagtgac gaaatacgtt tgaaatatcg
    35281 ttatttggat ctgcgtcgtc caaaaatgac taaaaacatc aaattaagaa atgcgacaac
    35341 aaaagcgatt cgtcattatt tagatgataa tgactttctc gatatcgaaa caccttacct
    35401 agggaaatct acaccagaag gcgcaagaga ctacctagtt ccttcacgtg tccatgctgg
    35461 acatttctat gcgttgccgc aatctcctca attgtttaaa caattgctga tgaatgctgg
    35521 attcgaccgt tattatcaaa tcgttcgttg tttccgcgat gaagatttac gtggagaccg
    35581 tcagcctgaa tttacgcaag tagatattga aacaacgttc ttaacagcag aagaaatcca
    35641 aacttacaca gagggattga ttgcgaaggt aatgaaagaa gttcgcggta tcgaagtcac
    35701 attgccattt cctcgtatga cctatgatga agcaatggct cgctacggca gcgacaaacc
    35761 ggatacacgt tttgatatgg aattgatcga tctaagcgat acagtcaaag aagtagaatt
    35821 caaagtcttc caaatggctc ttgaaaacgg cggtgtcgtg aaagcattga atgctaaagg
    35881 cgctgcagat cgctactcac gaaaagacat ggatcagtta ggtcaatatg taggacaatt
    35941 cggagctaag gggcttgcat ggctgaaagt agaagaagac ggattgaaag gtccaattgc
    36001 taaattcatg ggagaagcaa cagaagcaat cattaaagca acagatgcaa aaccaggtga
    36061 cttgctgatg tttggcgcag ataaaagtga aatcgtcgct gctgcattag gtgctatccg
    36121 gacacgttta ggaaaagaat taggactaat tgatgaatcg aaattcaatt tcttatgggt
    36181 aactgattgg ccacaatttg aattttcaga agaagaaggt cgatatgtct ctgctcacca
    36241 tccattcacg atgccaaaag aagaagatat cccgttattg gctgaagacc ctgcaaaagt
    36301 ctatgcagaa gcttatgata tcgtcttgaa cggttatgaa ttaggcggag gttctatccg
    36361 tattcatact cgtgagcttc aagaaaaagt actagaaact ctaggcttta caaaagaaca
    36421 aatggaagaa caatttggtt tcttgttaac agctttagat tacggattcc ctccacatgg
    36481 cggtattgca ttaggattgg accgttttgc aatgttatta gccggagaag ataatattcg
    36541 tgaagtcatt gcctttccta aaaacggaaa agcagcagat gtgatgagtg atgcaccaag
    36601 tatggtttcg cctttacaat tgttcgaatt gaatattgat gtaacgaata tagaagaata
    36661 gccgataaaa aggtaaaaag gatttgtcga atacgcttct tttgacaacc ttgatttaat
    36721 tgaaaaacac tttataatga agccataaca tataaagcgg ggcttttcca tgaaaaaaac
    36781 attcgaaaat tgggcttatc atgattacac gaccaagctt tcggtatcta tcgtttatgc
    36841 gttgatcgca tcagtcgcat tgaatttctt ttatcagcca ggacatattt attcaagtgg
    36901 tattactggt ctagctcaga ttttgacgac tttgtcaaaa tcactgatag gattcgaagt
    36961 accggtatca acaactttgt atttactgaa tatacctttg tttgtattgg cgtggttaaa
    37021 aatcgggaag aagtttacaa ttttcacttt tatcacagtc actttgacct ccctttgtat
    37081 ccatatttta ccggaaacag tcctatctac cgatccaatc atctgtgcgt tgtttggggg
    37141 ggctgtgatg ggactaggta ttgggctagc gttgagaaac ggcctatcat cgggcggtct
    37201 agatatcgtg agtatcacga ttcgtaaaaa aacaggaaag tcgattggtt ctatttcgat
    37261 ctacttcaat gcattgatcg tttttgttgc cggaattcta tttggctggc aatatatgtt
    37321 ttacagtacg ttgtctattt tcgtcagtgg aaaagtaact gatgtcgttt atactaaaca
    37381 aaagaagatg caggtaatga tcgtaacgaa acatccagaa aaagtagtaa atgaaatcca
    37441 aaactgtatg cgtagaggga tcacgatcat caatgaagca gaaggtgctt ataagcatga
    37501 taagcagaca gtattgatga cgatcgttac acgatacgaa ctgcctctac ttagagaagc
    37561 aatgaaagct tctgatccac aagcttttgt aagtattgca gaaaacgtcc agatacttgg
    37621 tcgtttctat gaggatggca tttagatcga gtttgacaaa aattcatgaa atactgctgt
    37681 agacagacgt tcgtttgtct gcagtttttt tctaccatag tagaatcagc aaagaaaaac
    37741 tgctttttta tttgaaattt tcttaaagtt gaaaaaaagc atgtttattt ttttaaattt
    37801 atgggatgat agaaaaaaat agtagagaaa ggacacgatt ctacttgcta ttttcactta
    37861 atacccgtat attacgaatg aaaaaagttt atgaaaagtt ggaatattga atggaaaaac
    37921 aaaaaaggtt aaaaaacagc cgctacatca ctgggtttga tgggctccgt tcattagctg
    37981 tggtgggtgt tatcttctat caccttctgc caactagtat gaaaggcggc tatttgggcg
    38041 ttccgatttt tttcgtcgta tctggttatt tgatcacgga tctgctgcgg caggaatggg
    38101 aacaaaatgg aaaaatcaat atttggcaat tttatgtcag aagaatgaag agactctatc
    38161 cggcacttgt ttttatgctt gtgacagctt cggcttatat cactttgttt cagcgagggc
    38221 tgttaaacaa tcttcgagga gttgtggtca gcagcttact gtatgtcaac aactggtggc
    38281 aaatcaataa cggtttatct tatttcgatc gatttgctaa tgagtcaccg tttacgcata
    38341 tctggtcttt agcagtagaa ggtcaaaact atttggtttg gccgatcttg tttgtattgt
    38401 tgatggtatt tgttcgtaaa agaaaatgga tcttttatac tgtgttaggt acatccttcc
    38461 tctccgccat cttgatgatg atcttgtata caccaggagg cgatccaacc cgtgtttatt
    38521 acggtaccga cactcgtctg ttttctatat ggttgggaag tgcgcttgcg tttgtttggc
    38581 caagtacgcg tttaaagaaa aacatcccga aacaagcgaa aaatattctg aatatcgctg
    38641 gaggaatctc gcttttggca ctgattcttt gcttcttctt tttagatgat cattacaatt
    38701 tcatctatta cggaggaatg ctcctagtga gtctgttatg cgtagtgcta gttgcagtga
    38761 cagcacaccc cggagcaagt ttgaatcgat ggctgacaaa tccagtattt acttggattg
    38821 gcaaacgaag ctatggcatt tatctgtatc agtttccagt tatgattttt tatgaagcaa
    38881 aggtgactga tattgccgat catgttttgt tgcatacttt agtggaaatc acattgattc
    38941 taatcatcag tgaactttct tatcgatatg tggagcgtcc gctagcacgt tttgattatg
    39001 gtcgtacatt ccaagtgatg aaaggatggt tcagcaaacc gatcatcagt aagagcaaac
    39061 catgggttgt tccaggcaca ttacttgtag caattgcact cgttggtttc gttacggcac
    39121 cgaaaaacgc tgtaacagcc gatcaaaaac aactgcaggc aaaaatcgct gaaagtaaaa
    39181 aattagcaga agaaaccaga aacaattcag aaaacaccac tagttcggtt gaccaagcga
    39241 tccttgataa gtacgagttg tccaaggcac aagggaataa ggcacaaaaa ttagaactaa
    39301 cggcatttgg tgattcagtt atgttggatg ctgcaacgga tctacaagaa ctttatccaa
    39361 aagtagtggt agatggagat gtcggacgac aattatatgc cagtgtaccg tacattgaaa
    39421 aattaaagga acaagatttg ttgaaagaca ctgttctgat tggactagga acgaatggct
    39481 cattctcgga agctcagttt gatagtgtga tggatgcaat cggtgatcga aaagtatact
    39541 gggtcaatgt ccgagtaccg acgaagcgct ggcaaaatga cgtgaatgcg atgcttgaac
    39601 agatgacgaa aaaatacgac aatatgacgc tgattgattg gtacagctac agtaatgacc
    39661 atagtgactg gttttatgat gatcaagtcc atccaaatcc tgaagggatg acacattaca
    39721 ttcacttagt cagcgaagcc ttgctaggag gaagttcaag tacgaacagc agtgagacaa
    39781 acgtgacaga atgatagcta caaaaaactt caggcaaaaa catttgtttt tgcctgaagt
    39841 ttttttatat ttaccgtgtt tttaacattc atatagattc aggaagcaaa acggttgagt
    39901 gtgtctgtaa ataacgttct ttttgttttc gggtgagttt tttgaatgca gcttctgctt
    39961 ttgttgcttc gcttcttgaa gaaaatactt ctgcatggat catcttaaca ggtcttcttt
    40021 tttgaagacg agtatattta gcaccagtac cattgttatg ttcctctaat cgtctagaaa
    40081 gatcggttgt atatccacca taaaatgtct gatctttaca gaataatacg taaaaataat
    40141 gttcatttgc catagagcat ctcacgaatc tctgcagtgt attctccatc catattgtaa
    40201 gtgatcaatg gtggagcgac tttgaatccg tctaatttgc cttctttgat tccttcaact
    40261 aacaaaatat ttgcctcttt tccaggtttt gggtaaacaa attggatcct tttaggtgca
    40321 attcgatagt cttccatgac ctgaatgatt tctaagaacc gatcaggacg atggaccata
    40381 gtaaagcgtc catttgtttt tagtagctta cttgaaatat cgatcacttc ttctaacgtc
    40441 gtatgaatct catgacgggc gattgccagg tgtttattag ggttcgtttt atttgttggc
    40501 agccctttga aataaggtgg attacagacg actaagtcac aagaattatg tcgaacgaca
    40561 gataagctgt ctttcagatc gatggtgtgg acagtcattt gttcatttaa atggttaagc
    40621 gcgatactac gtcttgccat gtctgctaga cgaggttgaa gttcgatagc gtcgatagcc
    40681 gcctgggttc gtttacttaa aaaaagtcca actgcgccat ttcctgcaca aaggtcaaca
    40741 atcttccctt ttttaggtac acgggaaaag tgagcaagca gcacagcatc taaagaaaaa
    40801 gaaaacactt tggaactttg gataatcttg atatcttctg cgtataattg gtcgattcgt
    40861 tcgtcttcgt gaagcatgga ttccctccaa aatgaaaaaa atagtcgtct gtattatact
    40921 atttttatta aaaagaacca aggtggcttg tcaaacctct attttttatg catactatga
    40981 ccataagaag ggaaaggaag agactatgtt ttatcgattt atgcgcgggg tagttcgagt
    41041 actattagcc ttagttaatg gaaatgcccg ctatgagaat aaagaagttt taccgcaaga
    41101 tgagaattac atactagttg ctccccatcg tacttggtgg gacccattat atctggcggt
    41161 agctgcagca cccaaggaat tcagctttat ggcaaaagaa gaactgttca aaaatccagt
    41221 tctgcgattt atattaactc gatccaatgc ttttccagtt aaaagagaaa atccaggacc
    41281 aagcgtcatt aaaacacctg taaagatttt gaaatcgaca aatttgagtt tgatcatgtt
    41341 tcccagtggg acaagacatg ctacagaact taaaggcgga atggcattga tttccagaat
    41401 gtctaaagcg aaaatcgtgc cagctgtata tcaaggtccg ttgacacttg gggatctgtt
    41461 caagcgtcag agagtgacag tgcgtttcgg cgaaccaatc gacttatcag atatcaagaa
    41521 aacggataaa gaaggtatgg cggagatcga acgacgaaca caagcagctt ttgacaaatt
    41581 ggacaaagaa gtgaaccctg atttcaaata tgaagtcaaa aaaaaataaa atgataagtt
    41641 tatgcgtggg cggtcttttg aatctactgt cctgttcagt tcagactttt tcaatgaaaa
    41701 cgagggagag acgtcaagtc tgccctcttt ttgcgttcca atatgtaaaa tagaagacag
    41761 gtgtcatgta atggatcaaa gtgttataat aattagaagg acattttttg aggtgattga
    41821 atgcgttttt tacatacagc cgattggcat atcggaaaaa aactacaagg ttatgacttg
    41881 attgaagacc agcgtcatgt actatcttct atactagata tcgcgaaaga agaaaaagta
    41941 gaggcaatcg tgattgccgg agacttatat gataggagtg tcccagctgt tgatgccgta
    42001 gaattataca atgaattgat ggtcgattgg aatttaaagg aaaagatccc gatttttgct
    42061 atatcaggaa atcacgatag cagtacgaga ctttcagcgg gtacgccatg gttcagttat
    42121 tcgaactatt ttttgcgaac acagctagca gaagcatttg aaccagtcga gtttaaaaac
    42181 gtacaatttt tctttcttcc ttactttgaa ccaattgcag cgagattata ttttgaagat
    42241 gacaatatcc gaacgatcaa gcaagcggta gccaaggtga tagataaaat gcaggagcta
    42301 tttgattcag aaaaaaaaca agtattgatt agccattttt ttgtaatggg aagtgtaaaa
    42361 tctgattcgg aaacgaaaat cgaagtaggc gggttggatt cgatccctgg tgaactactt
    42421 acaccgtttg attatgtagc actaggacat ttgcataatc aagctgcttt gagacaggat
    42481 aatgctcgct atagcggttc cccattgaaa ttctctttat ctgagatcaa ccaaaaaaaa
    42541 ggcgtatgga tcgttgacct gacaacaggc ctggagttga attttaggga gatcgaaccc
    42601 ctgcatgata ttgaagaaat aacagcgagt ttcaaagaat tgctggatcc tgccttttat
    42661 cagtccgtca atcgggagaa ttacatctat atctatctca ctgatagaac gatcatcccg
    42721 aatatgatga accagcttcg gcaaatatat cctcgtattt tggggattga acgggcaaac
    42781 ggtcgagcag aacaaacaaa gcgcaaacga aaagtactag aaatgaaaga tcctcaaaaa
    42841 ttagcagaag cattttttag tgaaatgacg gaagagcagc taactgcaaa ccaagaaaaa
    42901 tggctcggag aaacgttgca agaaatcatg gaaatggaat gatcaagaat gaaaccgaaa
    42961 aaattacaac ttaaaaattt tggtcctttt atcaatgaga ccgtagattt ttcacgtttg
    43021 acagaagcac cgcttttttt gatcagcggg aagacaggag ctggcaaaac gacgattttt
    43081 gatgggatca ccttcgcact ttttggtgaa acttccggaa gacttcgctc tggaaaagag
    43141 atgcgttctt tgtttgctac accagacgaa gaaacaagcg tgactttttc ttttgaacat
    43201 cagcagatga gctatgaaat cgaacgaaaa ccagaacaag ttttagcaaa acgaaaagga
    43261 aacggaacaa aaaaacaggc agccaaagta acgttgacca tttttgatgg gaaaggaaaa
    43321 gaaataaaac aaatcacaaa acgtacagaa gcagatcagt tgatcagaga actgatgaat
    43381 ctcgatgcga aacagttttc tcaaatcgtt ttactacccc aaggagaatt ccgtaatttt
    43441 ttggtttcat caagcagtga taaagagact gttttacgtc atttattcgg tactcaattt
    43501 ttccaacagt tcaatgagcg attaaaagaa aaagcaaaac aacagcaaca aaaattggat
    43561 catttagagc aagagctgca tcttctgcaa aaaaggtttg ttttagtcaa agaagaggaa
    43621 ccagcagcag ggtttgaagc cgttttagcc caatggacaa accatcaggc aatactcgaa
    43681 aaacaaatca cggaagaaaa agaagctcta acggaacttc aggaaaaaca aaagcaacta
    43741 gaaaattcct actatcgttt tgaaagcttg aaaaaaagtt atgaagaaaa aacgatatta
    43801 ataaacaagc aaaaggaatt agctgctcaa aaagacgaaa tagaggaaaa gaaacgatgg
    43861 attgggtatt atgaatttac gcaacgatta gtcgaaccaa tccagcgcct acaagaaata
    43921 aaagttgaaa aaatagaact gtcacaaaaa agtagtgagc aaacaaagaa actagcgatg
    43981 actacgcaga attatgaaga gtggcaaaaa aatgaagcaa accgcgtacg ccgacaaaca
    44041 aaaatcaaag aagctgctca aaaactgcaa aacaaacaga tgatggtgcc aattgtcgaa
    44101 tccttacgta gtaaaaaaca agacgcagaa caattaacaa tttcattgga taaagaagaa
    44161 ttgaatgccg agaagctgta tcaaaatctc gctatctatc aggaaagaac tcgaattctt
    44221 caagaaaatt tactggatca ggaaaaactg caagaagatc gtcttgcttt tggacagtta
    44281 aaatatctag atgaaaagca gcaagaaata gaaaaacaaa aagcaaaaga cactgggtcg
    44341 ctcgcacaga taagagaaga actcaaaaaa gcaactgaag aaataacaaa aaatagtcag
    44401 ctattgaaag aacagacaga acaagcagaa cagctgaaaa gccaatgggc aaagcagcaa
    44461 attgctcgtc ttcagctttt tttgcgtcca ggggagccat gtcttgtttg tggatcaacg
    44521 gaacacccca tacaacaatt aaaacacgaa cagccaaata aggaagatat cttaaaagta
    44581 gaacaacaac tggcagaagc tgagtctgct gtagaaagaa ctcagcaaat cattgccaaa
    44641 agtgaagcac atgaaaataa tcttcaacaa caattatcag atttagaaca aaaaatcatg
    44701 gcaacgaata tacaattggc tgaacaagaa gaacacttgc aacaggcttt gaaaaaggca
    44761 tttcctgaga taacagaaca aaaaacaaaa acaactcttg tagtaatcga acaatcttta
    44821 gatagacgaa aaagcaagat ggccaatgaa agaatagagc tggaagaaat aagacaacaa
    44881 gcagaaaagc tcgaacaaca attagctgtc tacaaacagc agcagacaga aaaaaataat
    44941 cagctccagc aactgcaagg agaaatcaaa agtttagaac aacaattgca agatacggat
    45001 agtaactgtt tagaagagga gatacgacga cttgaaaaac agttagaaga agactcgaat
    45061 caactagctg ttgagcagaa agcaggagtg caattgttgc aagatcttca tacgcttcgg
    45121 acacagcttg atcttcaaca tgaacaaata gaaaaaatag atgaaaaaat agaaaaggaa
    45181 caagaaagaa tagctaaaaa gttagaaagc cagtcgtatt tttcaacaat tgaagaaatc
    45241 tgtgcatttg cacaacagca agcacaatat aaagaaaacc aacgtacagt tacttcttat
    45301 catgaagagc aattagtgac agctgaccgg ctgcgtcaat tagaaaatat ccagctaact
    45361 gagacattcc cagatacaga agagatcagc caacagttag aagaaataag acgtatgatc
    45421 gaaataaagc aagaaaagat atacggttta caagaacaaa aaattagtaa tcaaaaaatc
    45481 tacgatgaat gtctttcgat ttatcacaca agccagacga aattagatga gcttacgcaa
    45541 ttacagcagc tttcgcaaac attcaacgga gaaaatccta agaagactag tttggaacgg
    45601 tatgtgcttc aggtttatct tcaagaagtg ttacaagtag caaatgatca tctccaacgt
    45661 ttaacgaaaa atcgttatca atttgagcta gcggatacca ttggcagtta tcgagggaaa
    45721 accggattag aaatcaatat ctatgatgat caggcaggta tgtctcgaag tgcgcatacc
    45781 ctgtctggcg gtgagagttt tattgcagca ctttctttag cattagctct tgccgatgtg
    45841 atccagacac aagctggtgg tgtagcaatc gatgcattgt ttattgatga aggatttggg
    45901 tcattagatg aagaagcttt ggaaatggca atggaagctt tagaaacgat cgaaagtgaa
    45961 ggaagaatga tcggcatcat tagtcatgta agagaattga aagaacgagt attacaacaa
    46021 ataagaattg atacagaagg tagcggacaa agcaaaatac gctatcaact tggataaaaa
    46081 gggagattgg taactatgaa atggagtatt ccagaaaaag tcattgcacg tggacgtcaa
    46141 tacttgcaag aaggccgagt gttatcggtt gtccctgacc aagaagaaaa cgtgtggcat
    46201 gcagaagtct tgggcagtga attgtatatg gttgatttgg atggtacagc aaaagagata
    46261 gattactgcc aatgtcccta ttgggaggaa cataaatatt gtaagcatac agttgctgtg
    46321 gaactttatt tacggtcgaa aaatctatct cgtatcatga aaaaagacca gccaatgcta
    46381 gaaccggtaa aagcaacaag tgagggagaa atcattgcaa aaggtttttc tcgtctaaaa
    46441 acacaaaaca atcctcaagc ggtgcaacct ttgattattg aataccaagt agaaacgatt
    46501 gagactaatc agtatcaccc agagctttct attttggcac tttacttgcg tatcggttca
    46561 ttggcatcac cgaaaaaaac atatatcgta aaaaacatct atgatttttt acaagcttac
    46621 acagaaaaag aaacttacac tgttaataaa cagtaccagt ttcgtctaga taaacgggcg
    46681 tttcaaaaag aagatcaaaa aatactcacg catcttgcag gaagtgctca gacgcatcaa
    46741 ttattaggta cgaacggctt acaagtcaaa ggaaaactag ataaaagata tctgcttctc
    46801 ccagtagaac aagcccgctt cctgttagag cagatgaatc agactacacg tttgcatctg
    46861 cagataaatg aacaaaacga tcaaggaata caattttctg atcaaaacaa acctttatca
    46921 ttcaaagtgg aaaaatttgg agaagattat ttgctgcaag cattgaatga ttttgatttt
    46981 ttctttatgc attatcagtg gggagttatc caagggacat tttattctct gacgaaatat
    47041 caacaaacgg tttatcaaac ttggaaacaa ttgattcgcc gattggagaa accagaagta
    47101 gtgttcaaga aaaaagaatt accgtctttg tttaaagaga ttatccctct gctgtcagag
    47161 atcggcgacg tagctattgc ccctgcagta gaagaggaag tagcagatat ccccgtcgaa
    47221 tttattttct ttttccgaaa agcaaaagga aaaatccaag ctcgagtcga tttcgtctat
    47281 ggtgaagtca tctattcgac tgacccgaag catgaggtct cgacagaaca ggcttttcag
    47341 gtgttacgtg acacagtaga agagaaacga gtacttgatc tattcagaat gtatcgctac
    47401 cagaaaaatg aaactggtta tgaacgtcca ttgccaagcg gggaagaact atatgccttt
    47461 ttccgcacag aactatctgt cttccgtcag tttggtgaag tcagacttgg aagaaaattg
    47521 cgagagttgt atttagatgc gcataaacat caaccgaaaa tcgaagtaac cgagaccggt
    47581 tcatggctcg atattcgatt cgatgtgaca gggatagagg agcaagaaat cgatcatgtc
    47641 ctgcaaagct tgctgagaaa tgatgctttt tatacattag aaaatggtca ggttctttct
    47701 tttgattcag aagaatttca acaaacaagc caagtgttgc aacaattacg ggaatcgata
    47761 cgtacagaag aaggaacgat ccatgtacct aaaaatcaag gattgatcat tcaaaatcaa
    47821 ttggaaaaaa gcaatgctac ttttagtgaa tcgttccaaa cgatggtaca agatctcatt
    47881 catccagaaa gataccaggc acagcttccc aaaggattga atgcaacgat gagagattat
    47941 caaaagcaag gttttcgatg gttgaagatg ttaggtcatt atcagtttgg cggtatttta
    48001 gccgatgaaa tgggacttgg gaaaacacta cagacaattg cctttttact ttcggaaaaa
    48061 gaagaacgga aaagcttctc tgctttgatc gtagcgcctg cgagcttaat ttacaactgg
    48121 caggcagagg tgagaaaatt cgccccttct ttaagtatcc aagtaatcaa tgggaataaa
    48181 aaagaacgtg aagaattact ggctaaagac acagatatcc gagtgacttc ttatgccagc
    48241 ttaagacagg atctagctaa ttatcagtcg caaaaaattg attatttgat tcttgatgag
    48301 gcacaaatgg tcaaaaatag cagtacgaaa actgcacaag cattgagaga attagctgta
    48361 cctcagcggt ttgcactgag tgggacacca atcgagaata atttagaaga attgtggtca
    48421 ttgtttgcaa cgattatgcc aggatttttc ccgactaaaa caaaattcag ggaacttgcc
    48481 gcagaagaaa ttgcgcaaat gattcgaccg tttattttac ggcgagataa acaaactgtg
    48541 ttgaaagact tgccggaaaa aacagagatg aatctatatt ctgcacttac agaagaacaa
    48601 aaaactgttt atttagctta cttgcgtcaa atgcgtgaag aaatcacatc aatggattct
    48661 gaagcattca agaaaaaccg gatcgggata cttgctggtc tgacaagact gcgacaaatt
    48721 tgctgcgatc ctcgtctgtt tattgaagat taccaaggcg gttccggcaa gctcgaacaa
    48781 gtaaaagatc tactggtagc tgccaaagag aacaaacgaa gagttttgct gttttctcaa
    48841 tttacaggga tgctgactat cttgcaagaa gagttagccg agctagggat ctcgacattt
    48901 tatttaagag gcagtacgaa accacaagat cggttaagta tggtagacgc atttaatgct
    48961 ggcgaaaaag atgtttttct gatttcctta aaggctgggg gaactggatt gaatctcacg
    49021 ggagcagata cggtcatctt gtatgacctt tggtggaatc ctgccattga agaacaagct
    49081 gctggacgtg cacatcggat cggacaaaaa aatgtggtag aagtgtggcg tatgatctct
    49141 gaaggaacga ttgaggaacg catggattca ctccagcaag aaaaacgcga actgttccaa
    49201 aaagtgatcc aaggtaatga agaacagttg acgaaactga ctgaagaaga catccgcatg
    49261 attttaagtg taggagaaat cgaggagtaa gagactcgaa aaagcgttca gtaatgggta
    49321 agaaagcctt tctaaagaag cgattgttta tttgatttaa aaaatagcaa ggaggctggg
    49381 attccatttt atcccagctt ccttgctatc atttttttat ttctattttt aaaggttata
    49441 caaagattgt cttcattgtc tctaacagct gatcaaaaga agaaatctcg atatcgcaaa
    49501 ttggagtctg ttcaattttg cgttctcgat ggttgaacca aagcgcttgc cagttagcac
    49561 tttttgcacc tagaacatca ttgtcgtaat tatcgccaac gtataaggtc tcttctggaa
    49621 gcatatgaaa ttctttctca gccagctgaa aaatctcttt ttctggtttt tggaaacctg
    49681 ttgcttgaga aatgagcata ttgcgggaag gaatccaatt gttcaattga agttgcttca
    49741 gtttttttgt ctgatggtca gtcggcccat ttgtgataat tcctaatggg acattctttt
    49801 ccttcaagaa atcgagggtt tttttgacct cctcatgcaa gcagatatta tcaagttctt
    49861 cctcgtaaat tttttgaaat aagaggccat cttcttccgt aatatgaggg tagtctaaat
    49921 cttttagtga ttggctgatc cgatgtgctc gcatatattc tagtgtccaa tctcctgcca
    49981 ttacttttgg gaagttttca tcactatgat ggcggaaccg gatatatagt ggatgcatat
    50041 ctgcatctga aacaagcgga acgactcttt tgaccgcgtt tcgaaatggc tgctgctgat
    50101 catacatcgt atcatcaaca tcaaacacaa ctgatttaat caaaatttta cacgcctttc
    50161 ggttttcatt gattttttca caaatccttg actattgtaa cagaaaaaat tgtctaaaaa
    50221 agctgataaa taaaggtttt tcttactata tttctatttc cttgacagaa taaggttttt
    50281 atatttcagt ttttttcact aaaaatcgtg aaacacgtca ataagtttga tgaacaaatt
    50341 aatcattatg gtagaagtcg attgatttat tttaatgcaa tctctgtgga attttcgaaa
    50401 gatttatttt tttaatacag tctcaatata actattcaaa cataaaata
//
LOCUS       DANKAZ010000010        48489 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000010 DANKAZ010000000
VERSION     DANKAZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..1040
                     /locus_tag="PWY67_000528"
     CDS             <1..1040
                     /locus_tag="PWY67_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2056836.1"
                     /translation="YTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKK
                     IENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQM
                     EVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPL
                     GSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVST
                     PADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQL
                     KNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSRI
                     DKVRYMFNKIT"
     gene            1138..1326
                     /locus_tag="PWY67_000529"
     CDS             1138..1326
                     /locus_tag="PWY67_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDL2056837.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1384..1737)
                     /locus_tag="PWY67_000530"
     CDS             complement(1384..1737)
                     /locus_tag="PWY67_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2056838.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2268..2690
                     /locus_tag="PWY67_000531"
     CDS             2268..2690
                     /locus_tag="PWY67_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2056839.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2687..2917
                     /locus_tag="PWY67_000532"
     CDS             2687..2917
                     /locus_tag="PWY67_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2056840.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3415..3615
                     /locus_tag="PWY67_000533"
     CDS             3415..3615
                     /locus_tag="PWY67_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2056841.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3642..4835
                     /locus_tag="PWY67_000534"
     CDS             3642..4835
                     /locus_tag="PWY67_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2056842.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4903..6468)
                     /gene="guaA"
                     /locus_tag="PWY67_000535"
     CDS             complement(4903..6468)
                     /gene="guaA"
                     /locus_tag="PWY67_000535"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2056843.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6527..7450)
                     /locus_tag="PWY67_000536"
     CDS             complement(6527..7450)
                     /locus_tag="PWY67_000536"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL2056844.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7646..8251
                     /locus_tag="PWY67_000537"
     CDS             7646..8251
                     /locus_tag="PWY67_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2056845.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8559..9575
                     /locus_tag="PWY67_000538"
     CDS             8559..9575
                     /locus_tag="PWY67_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2056846.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9716..11017
                     /locus_tag="PWY67_000539"
     CDS             9716..11017
                     /locus_tag="PWY67_000539"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL2056847.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11063..11725
                     /gene="deoC"
                     /locus_tag="PWY67_000540"
     CDS             11063..11725
                     /gene="deoC"
                     /locus_tag="PWY67_000540"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL2056848.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11746..12144
                     /locus_tag="PWY67_000541"
     CDS             11746..12144
                     /locus_tag="PWY67_000541"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL2056849.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12210..13292
                     /locus_tag="PWY67_000542"
     CDS             12210..13292
                     /locus_tag="PWY67_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2056850.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13504..15066
                     /locus_tag="PWY67_000543"
     CDS             13504..15066
                     /locus_tag="PWY67_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2056851.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15059..16192
                     /locus_tag="PWY67_000544"
     CDS             15059..16192
                     /locus_tag="PWY67_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2056852.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16192..17148
                     /locus_tag="PWY67_000545"
     CDS             16192..17148
                     /locus_tag="PWY67_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2056853.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17247..18413
                     /gene="deoB"
                     /locus_tag="PWY67_000546"
     CDS             17247..18413
                     /gene="deoB"
                     /locus_tag="PWY67_000546"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL2056854.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18437..19255
                     /locus_tag="PWY67_000547"
     CDS             18437..19255
                     /locus_tag="PWY67_000547"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2056855.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19272..19979
                     /gene="deoD"
                     /locus_tag="PWY67_000548"
     CDS             19272..19979
                     /gene="deoD"
                     /locus_tag="PWY67_000548"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2056856.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(20277..20963)
                     /gene="gpmA"
                     /locus_tag="PWY67_000549"
     CDS             complement(20277..20963)
                     /gene="gpmA"
                     /locus_tag="PWY67_000549"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2056857.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(21135..21716)
                     /locus_tag="PWY67_000550"
     CDS             complement(21135..21716)
                     /locus_tag="PWY67_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2056858.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21849..22532)
                     /gene="rpiA"
                     /locus_tag="PWY67_000551"
     CDS             complement(21849..22532)
                     /gene="rpiA"
                     /locus_tag="PWY67_000551"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL2056859.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22851..23264
                     /gene="rpsL"
                     /locus_tag="PWY67_000552"
     CDS             22851..23264
                     /gene="rpsL"
                     /locus_tag="PWY67_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL2056860.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            23348..23818
                     /gene="rpsG"
                     /locus_tag="PWY67_000553"
     CDS             23348..23818
                     /gene="rpsG"
                     /locus_tag="PWY67_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL2056861.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23912..25999
                     /gene="fusA"
                     /locus_tag="PWY67_000554"
     CDS             23912..25999
                     /gene="fusA"
                     /locus_tag="PWY67_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2056862.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            26171..27358
                     /gene="tuf"
                     /locus_tag="PWY67_000555"
     CDS             26171..27358
                     /gene="tuf"
                     /locus_tag="PWY67_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2056863.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27765..28073
                     /gene="rpsJ"
                     /locus_tag="PWY67_000556"
     CDS             27765..28073
                     /gene="rpsJ"
                     /locus_tag="PWY67_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL2056864.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            28104..28733
                     /gene="rplC"
                     /locus_tag="PWY67_000557"
     CDS             28104..28733
                     /gene="rplC"
                     /locus_tag="PWY67_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL2056865.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28761..29384
                     /gene="rplD"
                     /locus_tag="PWY67_000558"
     CDS             28761..29384
                     /gene="rplD"
                     /locus_tag="PWY67_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL2056866.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            29384..29674
                     /gene="rplW"
                     /locus_tag="PWY67_000559"
     CDS             29384..29674
                     /gene="rplW"
                     /locus_tag="PWY67_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL2056867.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29713..30546
                     /gene="rplB"
                     /locus_tag="PWY67_000560"
     CDS             29713..30546
                     /gene="rplB"
                     /locus_tag="PWY67_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL2056868.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            30589..30867
                     /gene="rpsS"
                     /locus_tag="PWY67_000561"
     CDS             30589..30867
                     /gene="rpsS"
                     /locus_tag="PWY67_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL2056869.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30890..31237
                     /gene="rplV"
                     /locus_tag="PWY67_000562"
     CDS             30890..31237
                     /gene="rplV"
                     /locus_tag="PWY67_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL2056870.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            31251..31907
                     /gene="rpsC"
                     /locus_tag="PWY67_000563"
     CDS             31251..31907
                     /gene="rpsC"
                     /locus_tag="PWY67_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL2056871.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31910..32344
                     /gene="rplP"
                     /locus_tag="PWY67_000564"
     CDS             31910..32344
                     /gene="rplP"
                     /locus_tag="PWY67_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL2056872.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            32334..32522
                     /gene="rpmC"
                     /locus_tag="PWY67_000565"
     CDS             32334..32522
                     /gene="rpmC"
                     /locus_tag="PWY67_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL2056873.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            32547..32813
                     /gene="rpsQ"
                     /locus_tag="PWY67_000566"
     CDS             32547..32813
                     /gene="rpsQ"
                     /locus_tag="PWY67_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL2056874.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32871..33239
                     /gene="rplN"
                     /locus_tag="PWY67_000567"
     CDS             32871..33239
                     /gene="rplN"
                     /locus_tag="PWY67_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL2056875.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            33276..33584
                     /gene="rplX"
                     /locus_tag="PWY67_000568"
     CDS             33276..33584
                     /gene="rplX"
                     /locus_tag="PWY67_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL2056876.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            33612..34151
                     /gene="rplE"
                     /locus_tag="PWY67_000569"
     CDS             33612..34151
                     /gene="rplE"
                     /locus_tag="PWY67_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL2056877.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            34170..34355
                     /locus_tag="PWY67_000570"
     CDS             34170..34355
                     /locus_tag="PWY67_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL2056878.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            34391..34789
                     /gene="rpsH"
                     /locus_tag="PWY67_000571"
     CDS             34391..34789
                     /gene="rpsH"
                     /locus_tag="PWY67_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL2056879.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34821..35357
                     /gene="rplF"
                     /locus_tag="PWY67_000572"
     CDS             34821..35357
                     /gene="rplF"
                     /locus_tag="PWY67_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL2056880.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            35518..35874
                     /gene="rplR"
                     /locus_tag="PWY67_000573"
     CDS             35518..35874
                     /gene="rplR"
                     /locus_tag="PWY67_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL2056881.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35895..36395
                     /gene="rpsE"
                     /locus_tag="PWY67_000574"
     CDS             35895..36395
                     /gene="rpsE"
                     /locus_tag="PWY67_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL2056882.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            36410..36589
                     /gene="rpmD"
                     /locus_tag="PWY67_000575"
     CDS             36410..36589
                     /gene="rpmD"
                     /locus_tag="PWY67_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL2056883.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36633..37073
                     /gene="rplO"
                     /locus_tag="PWY67_000576"
     CDS             36633..37073
                     /gene="rplO"
                     /locus_tag="PWY67_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL2056884.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            37073..38368
                     /gene="secY"
                     /locus_tag="PWY67_000577"
     CDS             37073..38368
                     /gene="secY"
                     /locus_tag="PWY67_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL2056885.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            38430..39077
                     /locus_tag="PWY67_000578"
     CDS             38430..39077
                     /locus_tag="PWY67_000578"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL2056886.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            39268..39486
                     /gene="infA"
                     /locus_tag="PWY67_000579"
     CDS             39268..39486
                     /gene="infA"
                     /locus_tag="PWY67_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL2056887.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            39519..39635
                     /gene="rpmJ"
                     /locus_tag="PWY67_000580"
     CDS             39519..39635
                     /gene="rpmJ"
                     /locus_tag="PWY67_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL2056888.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39653..40018
                     /gene="rpsM"
                     /locus_tag="PWY67_000581"
     CDS             39653..40018
                     /gene="rpsM"
                     /locus_tag="PWY67_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL2056889.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            40046..40435
                     /gene="rpsK"
                     /locus_tag="PWY67_000582"
     CDS             40046..40435
                     /gene="rpsK"
                     /locus_tag="PWY67_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL2056890.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            40515..41453
                     /locus_tag="PWY67_000583"
     CDS             40515..41453
                     /locus_tag="PWY67_000583"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL2056891.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            41481..41861
                     /gene="rplQ"
                     /locus_tag="PWY67_000584"
     CDS             41481..41861
                     /gene="rplQ"
                     /locus_tag="PWY67_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL2056892.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            42133..42972
                     /locus_tag="PWY67_000585"
     CDS             42133..42972
                     /locus_tag="PWY67_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2056893.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42948..43817
                     /locus_tag="PWY67_000586"
     CDS             42948..43817
                     /locus_tag="PWY67_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2056894.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43807..44604
                     /locus_tag="PWY67_000587"
     CDS             43807..44604
                     /locus_tag="PWY67_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2056895.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44807..45553
                     /gene="truA"
                     /locus_tag="PWY67_000588"
     CDS             44807..45553
                     /gene="truA"
                     /locus_tag="PWY67_000588"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2056896.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45886..>48489)
                     /locus_tag="PWY67_000589"
     CDS             complement(45886..>48489)
                     /locus_tag="PWY67_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL2056897.1"
                     /translation="PFLILALVYLSIGIYPGSSRSILASDAFSQFSNFHASFRNVLLG
                     KQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFPNQMMPDALYVLTLLKVGCAGLA
                     FWFLARTFKIPRWGHVALSVSYALISFITAHSEIIMWLDAFVYLPLVILGIHRVMDLK
                     RPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLLTNWSEYKKTIVPYGITSLLAGG
                     ASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDLIMKNMIGVYDTTKYGSIPFVYA
                     GLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASFYLVPLNLFWHGMHAPNMFLFRY
                     AYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAALFAVAWGIKPEGSYTYVTMTSF
                     VLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMSAEALVNTNSMIHGILDDWNYAS
                     RSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSANDSINYGYSGISLFSSIRNRNS
                     SSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYNISKNESTFSKYGFRQAGQSGDY
                     TLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNLINTLSGLNEQYFTFYTPTLKNQ
                     LNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQAYLSLFPTNFAQLESSSATITV
                     NGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGTKEISFMEPKVVGLDVVAYQAAM
                     NQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYDKGWKAKIDGKPVKVSNFKDAFL
                     MIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFILYVWYLDRNRPFYGTIDPAIEEQ
                     PRNTHRRRRRK"
BASE COUNT        15645 a         8892 c        10367 g        13585 t
ORIGIN      
        1 tgtatacttc aataaatggt gaattatgta agattgatag agcttattct ggagaaattg
       61 ttattttgca aaatgagttt ttgaagttaa atagtgttct tggagataca aaactattgc
      121 cacagagaaa aaagattgaa aatccgcacc ctctactaca aacaactgtt gaaccgagta
      181 aacctgaaca gagagaaatg ttgcttgatg cccttttgga aatctcagat agtgatccgc
      241 ttctacgata ttacgtggat tctacgacac atgaaattat actttctttc ttagggaaag
      301 tacaaatgga agtgattagt gcactgttgc aagaaaagta tcatgtggag atagaactaa
      361 aagagcctac agtcatttat atggagagac cgttaaaaaa tgcagaatat accattcaca
      421 tcgaagtgcc gccaaatcct ttctgggctt ccattggttt atctgtatca ccgcttccgt
      481 tgggaagtgg aatgcagtat gagagctcgg tttctcttgg atacttaaat caatcatttc
      541 aaaatgcagt tatggaaggg atacgctatg gttgcgaaca aggattatat ggttggaatg
      601 tgacggattg taaaatctgt tttaagtacg gtttatacta tagccctgtt agtactccag
      661 cagattttcg gatgcttact cctattgtac tggagcaagc ctttagaaaa gctggaacag
      721 aattgttaga gccatatctt agttttaaag tttatgcacc acaggaatat ctttcacggg
      781 catataacga tgctcccaaa tattgtgcaa atatcgtaaa tactcaactg aaaaataatg
      841 aggtcattat tattggagaa attcctgctc gatgtattca agattatcgc aatgatttaa
      901 ctttttttac aaatgggctt agtgtttgtt tagcagagct aaaaggatat caggttacca
      961 ctggcgaacc tgtttgccag acccgtcgtc taaatagtcg gatagataaa gtaagatata
     1021 tgttcaataa aataacttag tgcgttttat gttgttatat aaatatggtt tcttattaaa
     1081 taagatgaaa tattctttaa tatagatttg aattaaagtg gaaaggagga gattgttatt
     1141 ataaactaca agtggatatt gtgtcctatt tgtggaaata aaacaagact acgaatacga
     1201 gtggatacta tacttaaaaa tttcccttta tactgcccca aatgtaagaa cgaaacttta
     1261 attaatgttc aaaaaatgaa tataataaca atcaaagagc cagacgccaa gacgcagagc
     1321 cgataatttg agaaatgaaa ctctcatctt atcggctctt tttgtttatc tgaattttac
     1381 tgactagcct tcaatatttc ttgcttcatt gatacctttt gctagtgctt ccattaagga
     1441 tagttctttg tctgtaaagc tatccatgta tttttctacc tgtaaccttc gagtgctttt
     1501 aatcaaatta tctgtaggta agaaaaattc atcaatagat acatggagta atgatacaag
     1561 atcatacagc acttgtgtac ttgggtgttg tcctttgttt tctatatttg ttaaataacg
     1621 tgggtcaatt tcaatcattg ttcccacttg ttcacgagtg agaccccgtt tcattcgagc
     1681 ctctttaatc gctaaaccaa cggctctaaa atcgtattta tcttcttttt tacgcatact
     1741 aaatcacctc tatacatttt actgttccta tataaccata aacaggtata gaaaaacatg
     1801 ttatatagtt tatgagttcc tatagaacac tgtggaattt attaatgcga tgaatccaat
     1861 ttcaaataga aaggtcgctg attttattta cctatacaac tttataaaat ttagtcatac
     1921 tcctagaaag tagataaaat tggcgactta atggagggaa aagtcgccat gagaaagata
     1981 atctgtcatc tagttctctt aattaagttc ttatcaaaaa aacttatcca cctgtactgg
     2041 atagatatga ccatcaatgt ggttttgcag attacataga tagaaaaatc aaagcatatg
     2101 aacagagaaa acaatctgtt tatgtgcttt tttggtcact taataaatat cttttaggat
     2161 ttctcttcat ttatgcaaca aatcccccgt tcgtgtcggg gtttagtatg aagggagata
     2221 aagaagcaca tgccactccc tttcacgaca gaaagggggt gaaaagcatg aaaccatctt
     2281 cttttcaaga aaccatagaa aatcagtttg actatatctg caaacaagtt attgaagatg
     2341 aacgaaaaga ttacttcaaa catctagaca gactatcagc aaaagaagtc tcgttttctg
     2401 atatagataa ctaccttgtc aactcttttt ctactgttga tacgtacgtc actgatttcc
     2461 aactatttaa attatttgga ttaagaatca atgttgaaag tgatttatta agtgaagccc
     2521 tgcgtcatct ttcagagaaa aaacggaaca ttattttact tcattacttt atggatatga
     2581 gtgatgtgga gattgcggag ttactaaatt tgaatcgttc gactgtttat cgtcatcgaa
     2641 taagtggact agccatgatt aaagaattca tgaaggagtg taaagaatga aaacagctta
     2701 tccaagggtt cccttccccc tcattgtaaa ggcgacagat ggcgacgtgg aagcaattaa
     2761 tcagattgtt aaacattatc gagggtatac ctcaaaacgt tcattacgtc gcatgacaga
     2821 cgaatatgga aattctcata tggttattga tgaaacccta cgtggcagaa tggaaacgaa
     2881 actcatctct aagattttat cgttcgaaat taaatagtcc cattttttcg tggaagcgtg
     2941 tcgaatcgta tccacgcttc ccgatttggg aggtttgtta ttccatcaaa gcatattgtg
     3001 tgttcagtag gttttgatag gctaacaagc caaacgttct ttgacaacca aataaaggca
     3061 atcgaatacg tttcggtaag aaaagagctg atgaactaac acgccatgac ctacaaaatt
     3121 agtgagcgat tcatgttagt aattcgaaag taatctttag caggattgct gccatgactt
     3181 tcttatcgtg ataatgatac tcccgtatag ccatagtccg agcgttagaa gcgtcacagg
     3241 caatgggtac ggctacatga gaaccatgca gaagtgaaat tcctgtgagc tttgctagag
     3301 ctgttcgatt gctaagatat tcttctcttg tcagtgtatt attataaaac tatttgatat
     3361 aattataggt gaatattgga gttttcctag cctataattg ggaaaggagt aataatgata
     3421 acggtcacaa aaaaggattt agttgagcta ggatatggaa cttctttttc agcagacatc
     3481 ataaaaaaag caaaagaatt gatgattaca aaagggcata tctactatca atctcgaaag
     3541 ctagatagag tccctcgtga agctgttgaa gaaattctag gaattcggtt ttcaaatgaa
     3601 aacaggtctg agtagttttt gcacagtaag gagtaaaaac tatggttaaa gacccaataa
     3661 aaaaagcaaa gaatggaacc tattattttc gtgcaaatct aggttatgat tctaaaggga
     3721 aaaaaattca aaaatatcgt agtggattca ctactaaaaa agaagcaaga gaagaatatt
     3781 cgaaactgct attgatgaaa ccagaagaat taagtgagaa taaggataaa atgacgttta
     3841 aacattatat ctttgaaatt ttccttccat ggtataagac acaggtaaag ctcagaacat
     3901 atgaaaatcg cttaccaact attaagaagc atttctctta ttttgataaa atggctgttt
     3961 ccgatattga gcctattgat gtgcaaaatt ggcaactaaa attatcaaag aagtgtaagt
     4021 cttcttatgt aagagctgtt caaggactat tttcagttgc tatggatcga gcaattgtcc
     4081 ttgggataac caccacaaat ccatctaaga ttattggcaa tgtgaaaaag caaaagtcta
     4141 aaattgaatt ttggacaaaa gaagaatttg aaaaagtaat ctctctaatt tacaaagaag
     4201 attactatca acacttttta tttatctctc tgtggttttt atttatgact ggaatgcgaa
     4261 ttggtgaagc tactgccatt caatgggaag atattgattt tgactcagga gtactaacca
     4321 tcaacaaaac actctattac aagaatcaat cgaattatag atttgttgaa ccaaaaacaa
     4381 aggcgagcgt tcgtcatatt gtattagata aatgcacatt aacttattta tccgaatgga
     4441 aggaagtaca acaaaatcta attcagacag actttgtaat gagctataac ggtattccta
     4501 cccaaaaaca tacactatca tatgctattg aacgctatgc taagctggct ggtatccatc
     4561 gaatcagaat ccatgggtta cggcattctc atgcttcctt gttaatcagt atgggagaaa
     4621 atccacttat cattaaagat cgtctaggac acgaagatat tgagacgact ttaggaacgt
     4681 atggacatct ttatcctaac agtaatttcg aggtagctaa caaattaaat ggagccattt
     4741 cctttaaaga agcaaaacaa aattatgata cttcaccaaa gaatcaattt actgtagact
     4801 acttgcgaaa caaagaaatg aaaaaagtgc aataaaagtg cagtaaaaag ataagaagag
     4861 ccagaaaccc ttggtataag aggatttcta gctctcctac aactattccc actccactgt
     4921 tgctggtggt ttagaagtga tgtcatacac gatacggttg acgtgggcta cttcgtttac
     4981 gatacgtaca gagatttttt gcaagacttc ccatgggata cgggcaaagt cagcggtcat
     5041 tccgtcgatt gaagttacag cacggatacc gactgtgtag tcgtaagtac gtccgtcacc
     5101 cataacaccg acagagcgga tccctggaag aacagtgaaa tattgccaga tatcacggtc
     5161 aagtccagca gctgcgattt cttcacgcaa gatagcatca gattcgcgta cgatttctag
     5221 cttgtcttca gtgatttcgc caagaacacg aataccaaga ccaggaccag ggaatggctg
     5281 acgccaaacg attgaatcag gcatgcctaa ctgagtccct aattcacgga cttcgtcttt
     5341 gaataatgtg tttaatggtt cgatcaattc aaattgcata tcttctggca gtccacctac
     5401 attatggtga gatttgatcg tctgtgctgt ttctgtacca gattcgataa cgtcagtata
     5461 caacgttcct tgtgctagga aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc
     5521 atcaaaaaca tatacgaatt cattcccaat gattttacgt ttttgttctg ggtcagatac
     5581 acctttcaac ttgtctaaga agcgatctct tgcatcgact ttgatgatgt tcaagccgaa
     5641 ttttccgcct agactttcca ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc
     5701 aacaaagata gatgtcaatt ggtcaccgat cgctttttgt aaaagaaccc caacgacact
     5761 agaatcaacg ccacctgaaa gaccaagcaa gacttttttg tcaccaactt gttcacggat
     5821 tttttctact tgcatatcga taaagttatc cattgtccag ttaccttcac agtgacaaac
     5881 atcaaaggca aagtggcgca gcaattcgat tccgtattcg gaatgacgta cttctgcatg
     5941 gaattggata ccataaaaat tacgtgttgt gtcttggata gaagcaatcg gacaatcttt
     6001 gcttgttccg actgtttcaa agccttcagg aactttagtt accaagtcac catggctcat
     6061 ccatactgtt tgttgttttg gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc
     6121 taacaaggct ttcccatatt cgcggttttt agcaggctct acggaaccgc caagtttgta
     6181 cgtgatcaat tgcataccat agcaaatccc taatacagga atacctaaat tgaagatctc
     6241 aggatcgatc ccaaatgcat tctcatcata tacactgttt ggtccgccag aaagaatgat
     6301 tccctttgga ttcatcgctt ttacttcttc tgcagtaatg cggtggctca ataattcgga
     6361 aaagactcca agatcacgaa tacgtcttgt gatcaattgg ttatactgac taccgtagtc
     6421 taatacgata atcttttcaa cgttcgtcat atcggcaacg tttgtcaaat ttattacccc
     6481 tatcccttta aattttatct caaaatagtc aaactatttt aaaatgctaa tattttcgca
     6541 tgaaaatcga atcgatgata tgattttcag ttttatggag aattatatcg gctcttcctc
     6601 gtgtaggcaa gatatattcc tgtaaatttt tcaaattcac atttttccat acatcttttg
     6661 ccattttaaa ggcttcttca cgatctccga tagcaaaacg ataataataa ttctctggat
     6721 tttggaaagc cgtatccagt aacgcaccaa aacgatccaa ataccatttt tcgatcagca
     6781 cgggatcggc atcgacatag acagaaaaat caaaaaagtc gctgacgtag atttgctgat
     6841 tcgcaggtaa ttgaagcgta ttgatcccct caacgatcaa gatatcaggc tgctggatca
     6901 attcgtactt gtctggaatg atatcataca catcatgtga gtaaacaggt acttttatct
     6961 cttcctgacc agattttaca cggttcaaga aatcgatcag catctccata tcataacttt
     7021 caggaaatcc tttacggtcc atgattccac gctcttttaa tatacgattt ggataaagaa
     7081 atccgtcagt cgtgatcagc tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct
     7141 gcagcagtct ggctgttgta ctttttccaa cggctacact tccggcgatc ccgataataa
     7201 aaggcggtac ttctacgtat ttatgaagaa ataaaccttt actcaagaac aaagattcaa
     7261 attctttcat ataaagataa atcagatgag caagaggtac atagatatct cgaacatcct
     7321 gcatggaaat gcggtcattc aaacttttga tattatctag ttcatcttgt gtcaaaggtg
     7381 cttgcccatt acgataaaat tctcgccatt cttctcttgc cacacggtag tagttcattg
     7441 gatcattcat attagcctcc tgcgttctcc catcaatgga actattttaa cacaaaataa
     7501 acgcaaaccc caccacttaa gagagattgc cgatcttttt gtataatctt tataatagaa
     7561 agaaacggag tcttattctt gacttgcgca cggatacata aaaggataga ctgttgtcat
     7621 gaaagaaatg agaaggatgg aagacatgac aaatcattac tattctgaac gcccagatac
     7681 ggcgcacgat tttgaacaat gggcttttga gttaaaaggc agaacctttc aatttgtaac
     7741 agattctggt gtattttcac gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc
     7801 tttcgattgg gaagagttac ctgatgaagg aaagatctta gatgtgggat gcgggtatgg
     7861 accgattggg ttagctttgg catttgcgac acagcggttt gttgaaatgg tcgacattaa
     7921 tgaacgagcg gtaagtctag cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga
     7981 tatccatcaa tcgaatatct atgaagcagt tcatgaagaa acctatgcag ctatcgtaag
     8041 caatccgccg atccgtgctg gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc
     8101 tcggttgaag aagggcggta cactgacaat tgtgatccag aagaaacaag gagcgccgag
     8161 tgcgcaaaag aaaatggaag aaacatttgg taatgcagaa atcgtcacaa aagataaagg
     8221 atattatatc atcaaaagtg tgaaagaata actagctgaa aaatagtgga gcgacgaaag
     8281 agtggaactg ggcgtgcatg tacgggtacg tatcagttct ttttttgttg agaagttttt
     8341 aaaaacaaag aactattttt tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt
     8401 acgataaaat aaataagata cagaacttgt atagaaggct gccaagtcgg tagataaagg
     8461 aggtttttat caaaaaacat actttccttt tttctctatt cttttcttgg gggttttggt
     8521 acactgatag ggaatttaga tagaagggag ccaataggat gaagctgacc atcagagata
     8581 tcgcagaaat ggcaggcgta tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa
     8641 gcagatttag tgataagaca agaaaaaaag tgttgtcgat cgtgaatgaa catcattata
     8701 agcccgatta tttcgcttca aatgtgatca atcgacattc aaagaccatc ggaatgatcg
     8761 tccctgatgt cactgatttc ttcttctcca aactgatcga aggtgtagag agctatttga
     8821 atcctttggg ctatgtgatc atgttatgca attctcgtca tagccaagaa aatgagatca
     8881 agtatttaca agagctttcc caccgctcag tggacggaat cttgctggcc acaccaaata
     8941 ttttacctga gaaatatgca ttagacagcg gtttttatca aaaaatgcca atcattttaa
     9001 tcgatcgcgg attgaatcgt cgggataacg gacggcttat cgtaaaggaa tatgagggcg
     9061 cctatcaagc tgtttcgctg tttattgaaa atggccatac gaagatcggc atgctgaagg
     9121 aaaccactgg atactatcaa ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag
     9181 atcatggcct gccttttaat gggaaatttg ttgaacatgg agagttgact gtccaaggtg
     9241 gatatactgc atcaaaaaaa ttactcaaac ataaagatat cacggctatt ttttgcggga
     9301 atgatgccat ggcgatcgga tgctatcagg cgatcgatga attgggcaaa aaaatcccag
     9361 aagatatctc agtcatagga ttcgatggat taaagctgtc agaatacatg attccgcaat
     9421 tgacgacagt gcagcagcca agttttgata ttggatttta tgctgcacgt ttcctgatcg
     9481 atacgatcga atttcctcaa agaaaagttc cgaacaaagt gttcgaaaca aaattaatta
     9541 ttcgtgagag cgtaaaagta ttgacaaagg gctaactcgg cggtatattg cttatgtaag
     9601 catttacaaa ggctagttag tgcttttttt atttttagta aaccagttta ctaaagtggt
     9661 ttacctttcc taaaaataat gtataatacc gaatgaagac aaggaggatc aggcaatgcg
     9721 aatggtggat ctgattgaaa aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga
     9781 gtacatcgtc acgaactata cgaatggaaa gattccagat tatcaagtca gtgcattatt
     9841 gatggcaatt ttttatcaag acatgacaaa tgaagaaatc acgaacttaa cattggcgat
     9901 tgctaattct ggtgatgtca tcgatttgag ttctcttgaa ggaataaagg tagataagca
     9961 ttcgacaggt ggcgttggtg acacgaccac attgatttta gcccccctag tagctagtgt
    10021 cggagtgaca gtggctaaaa tgtctggacg cggtctaggt tacacaggtg gtacactaga
    10081 caagctagaa gcaattcccg gatttcagat cgagctttcg gatgaagaat ttgtacgcat
    10141 tgttaatgaa agtaaagtag cggtaattgg tcaatcagga aacttggcac cggcggacaa
    10201 aaaattgtat gccttgcgtg acgtcacagc gaccgttgat tctcttccgc tgattgccag
    10261 ttcgattatg agtaaaaaaa tcgcggcagg agcagatgca atcgttttgg atgtgaccac
    10321 tggagacggt gctttcatga agaacatcga agatgcaaga cgtctggcaa agaccatgac
    10381 gagcattggg aaactggcta accgagaaac agtggcggtt atctcagata tgtcagaacc
    10441 tcttggcgaa gctatcggaa atagtctaga agttgtcgag gcaatcgaaa cattacaagg
    10501 aaatggtcca gaagatctag tagaaatgtg ctacgcctta ggaagccaaa tggttgttct
    10561 tgctggcaaa gctaagacaa ccgatgaagc aagaacgttg cttcaagaag ctctggaatc
    10621 aggtaaagcg ttagcaaaat tcaaagaaat gatccaaaat caagggggag acccagcgat
    10681 cgtggaacat ccagaacgaa ttttgactgc acgctacaca atggagcttc ctgctaaaca
    10741 aagcggagtt gtgtcaaaaa tcgtggcaaa tgaattagga attgctgcaa tgatgctagg
    10801 tgctgggcgt aaaaccaaag aagaagatat tgatcacgct gttggattga aactgcataa
    10861 aaaaattggt gacactgtga caaaaggtga gtcactattg acgatctata gtaacgacga
    10921 agaaatcact tctgtcattg aattattata taaaaatatt gaaattggtg aatcggcaat
    10981 gaagccaacg ttgattcatg acatcatcac agaataaagg aaaaaagcgg actttaccgt
    11041 tttaaagaaa aggggaaaag aaatggaact aaaccgaatg atcgaccata ctattttgaa
    11101 agcagacgca tctaaagaag ctgtgatgca aattatcgaa gaagcaaaaa aataccattt
    11161 ttattctgtc tgcatcaatc cagcatgggt tgcgctagcg aaagaacaat tacaaggtga
    11221 gccagtagcc gtatgtacag taatcgggtt tcctctagga gcaaacacac ccgagacaaa
    11281 agcatttgaa acaacagatg ccatcaataa cggtgcagat gaagtcgata tggtcatcaa
    11341 tatcggtgct ttgaaatcaa aggatgatca acaagtccaa aaagatatcg aagcagttgt
    11401 tgaagcagca aaagatcgtg cgcttgtcaa agttattatt gaaacatcgt tattagatag
    11461 agaagaaatc atccgagctt gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac
    11521 atcaactggt ttctcaacag gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac
    11581 tgtaggacca gaaatgggtg tcaaagcttc cggcggtatc cataacgaag aagaagcaat
    11641 ggcaatgatc gaagctggtg ctacaagaat cggaacaagc gcaggagtag caatcgtatc
    11701 tggttcaact ggtgaaggct attaattttt tcaaaggaga agctcatggc agaactcaaa
    11761 caagaatgga tcaacacagc tattgaagcg ttagataaag cctatgttcc ttattcccat
    11821 tttccggtag gcgcttgtct cgtaacggaa tcaggcaaga tttatcaagg catcaacatt
    11881 gagaatgcat catttggatt aacgaattgt gccgagcgca cagctttttt caaagcagta
    11941 tctgaaggag aaaaaacatt tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc
    12001 tctccttgtg gtgcatgtcg acaagtaatg gcagaattct gttcccctga tatgccggtt
    12061 acgctagtag gagacaatgg tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat
    12121 gcatttactg aaaaagatct ataactcatg ttcattcagt cagctgactg ttgaaaaata
    12181 aaacaattat tttaggaggc tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg
    12241 ctgtcgcact agcagcaggg ttatttttag gtgcatgcgg caacaacggc tctactgatt
    12301 catccggtgg caaaagctca tcagacacaa caactgcagc attgatcaca gacacaggtg
    12361 gtgtagacga ccgttcattc aaccagtcag cttgggaagg actagaaaaa tgggggaaag
    12421 atcaaggact ttcacgagga aatgatggct tccaatattt ccaatcttca aatgaatcag
    12481 attacatccc aaatattgac caagcattga atgctggatt caaaacgatt tttggtattg
    12541 gttataaact aaaaccagcg atccaagaac aagcaacaaa caatacaggt acgaactttg
    12601 ttattatcga tgatgtcatc gatggattag ataacgttgt atctgcaact ttcaaagaca
    12661 acgaagcttc ttatcttgca ggtgttgcag cagcttatac gactgaaaca aacgtagtcg
    12721 gctttatcgg cggtgtaaaa ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg
    12781 gtgttgatgc aggtgcgaaa gaattaggaa aagaaatcaa agtattgaat caatatgctg
    12841 gtgacttctc tgctccagat aaaggacgtt caattgctca aggtatgtat gcacaaaacg
    12901 cagatatcat cttccatgct tcaggcggta caggtaacgg tgtattccaa gaagcaaaat
    12961 cattgaacga atcaggcgac aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag
    13021 acgaaggcga atacacatta aacggcgaaa agaaaaactt cacattgaca tctacactga
    13081 aagcagttgg aacagtagta gaagatttgg cacaaaaatc agcggacggt aaattccctg
    13141 gcggcgaaca cactgtttat ggattgaaag aagacggtgt tggtctaaca gaaggacaac
    13201 tttctgacga agcgaaaaaa gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg
    13261 taaaagttcc agaaactcct gaagaaaact aaaaccattt ctatagcctt gttagacaca
    13321 tgtttgacaa ggctatggtt tgattagaac aagaattgtt catttatcca agctgccttg
    13381 tgacagtttg gataagggaa caactccctg aagtaatgtt tgtttcaaaa gaagaacgct
    13441 cactaagcgt gaatcttagt caatcgttgc cctagataga aatcgttagg atgtgaaaaa
    13501 aaggtggttc aagaaaatat tgtcatagaa atgcagaata ttaccaaaga atttggaaca
    13561 ttcaaggcaa atgacaacat caatttacaa gtaaaggcag gagaaattca tgcgttgctt
    13621 ggagaaaacg gtgctggcaa atctacattg atgaacgtgc tttccggatt attagagccg
    13681 acatcaggga aaattttgat gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc
    13741 aaccaattag ggattgggat ggtccatcag cactttatgc ttgttgatgc ctttactgta
    13801 acagaaaaca tcgtgttggg aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa
    13861 gcacgaaaag agatccaaaa agtttctgaa cagtatggat tgtcagtcaa cccagatgct
    13921 tacgttcgtg atatttcagt tggaatggaa caacgggtag aaattttaaa aacactttac
    13981 cgaggagcag atgtactgat ttttgatgag ccgacagctg tattgacccc tcaagaaatt
    14041 gatgaattaa tcgtgatcat gaaggaatta gtcaaagaag gcaagtcgat cattttgatt
    14101 acgcataagt tagatgaaat caaagcagta gctgaccgct gtacagttat ccgccgtgga
    14161 aaaggaatcg gtacagtcaa cgttaaagac gtaacctcac aacaattagc cgatatgatg
    14221 gttggaagaa cggtttcatt caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt
    14281 ttgtctattg aaaatctagt ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg
    14341 aacttagagg ttcgtgctgg cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag
    14401 tcggagttga tccaagcttt gactggtttg cgaaaggcag aaagcggaca tatcaagcta
    14461 aaaggggaag acatcactaa taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac
    14521 gtgccagaag accgtcataa atacgggtta gtcctagata tgacattatc tgaaaatatc
    14581 gcactacaaa cctatcatca aaaaccttac agtaaaaacg gcatgctgaa ttattcagta
    14641 atgaatgaac atgccagaga attgatcgaa gaatatgatg ttcgaacaac gaatgaactt
    14701 gttcccgcaa aagctttatc aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc
    14761 gaccgagatc ctgatctgtt gattgttgca aatccaactc gtggactgga tgtaggtgcg
    14821 atcgaattta ttcataaacg tctgatcgaa caaagggaca aatacaaagc agtgttactg
    14881 atcagtttcg aattagaaga aattttaaat gtttcggatc gtattgctgt tatccatgaa
    14941 ggagaaattg tcggtatcgt tgatccgaaa gaaacatctg aaaatgaatt aggtctattg
    15001 atggcgggct ataccttaga agaagcacga aaagagttag aaaagggggc tggcgcacat
    15061 gaatgatcgc agcgaaaaat tccgtaattt attagtgcct gtactttcag ttgttttagg
    15121 attgatctta ggcgcgatat tgatggctgc ttttggtttt cagcccatcc aaggttattc
    15181 ttctatgcta gatgcagccc ttggttcgca aagagggata ggtgaaacgt taaggcaagc
    15241 aacgccgttg atttttacag cattaggttt ttctgtagct aactcagctg ggtttttcaa
    15301 tattgggctt tctggacaag cactttgcgg gtggatcgtc agtgtctggg tggcacttgc
    15361 gtttccagat ctgccaaaag ttgttttgct tccgttatgc gtgatcttag gtgccttagc
    15421 tggtgcgatg gcagcggctg ttccaggttt acttcgagca ttttttggaa caagtgaagt
    15481 gatcgtaaca atcatgatga actatatctt attatacgta agcacatttg ctttacatga
    15541 agtgatgcct gaaagctatc gctcaagttt ggactcttcc aatatgatca gtcaaaatgc
    15601 ctcactgcgg ttaccatggt taacacaagc ttttggcggc tctcgcgtaa actcaggatt
    15661 atttttagca atcatcgcgc ttattttcgt ttggctgctg atgaagaaaa cgactttagg
    15721 atttgaaatc cgttctgttg gattgaatcc ttttgcttct gagtatgcag gaatgagtag
    15781 taaacgtacg atcgttttgt cgatggtcat ttctggcgct ttggcagggc ttggcggtgt
    15841 ggttgaagga ttaggaacgt atcaaaactt ctttgttcaa acgacttcgc tatctattgg
    15901 atttgacgga atggctgttt ccttgcttgg ttctggatca gcaatcggta ttcttttatc
    15961 agcgatcttg ttcagtattt tgaagatcgg tggacttggc atgcagacag gagcaggtgt
    16021 tccgtttgag atcgtcaatg tatcgattgc tttgattatc ttctttgtcg ggatcaactt
    16081 cctgattcgt ttcgtcatgg ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc
    16141 gacaatagaa tcgaaaccaa cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact
    16201 atcgaattaa ttgccttgat ccttacttct acactggttt attcaacacc gctaattcta
    16261 acgtcactag gcggaacttt ttctgaacga agcgggatcg taaacgtagg gctagaggga
    16321 atcatggtca tgggtgcttt tagttccatc gttttcaact tgacgatgag tccatcactt
    16381 ggttctatga caccatggat cgctatttta gtgggtggcg tcgtaggtat cctattttct
    16441 ctgattcacg cagtagcaac agtcagtttg cgtgctgacc atattatcag cgggacagta
    16501 attaatctga tggcacctgc tttaggtgtt ttcttgatca aagcatggta tggtaaaggt
    16561 caaactgata acatcacgca gaatctgggg tatttttctt tcccaggatt agcagatatt
    16621 ccggtcattg gaccaatttt ctttaaaaat acattcttac ctgcttattg tgctattttg
    16681 attgctgtcc tagcttgggt gatcatcttc aagacgaaat tcggcttgcg cctccgttca
    16741 gttggtgaaa atcctcaagc tgccgacaca ttaggtatca atgtatatgc tatgcgttat
    16801 tcaggcgtac tgatttctgg cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg
    16861 atttccggta acttttcagc tggtacgatc gttggacaag ggttcatctc catggctgct
    16921 atgatctttg gtaagtggaa tcctttaggg gcaatgggtg cctcattgtt ctttggtttt
    16981 gctcaaagct tgagtatcat tggcgcacaa ttaccagtta tttcttcgat tccgccagta
    17041 ttgctgcaaa ttgctccata tttgctgaca atcattgtgc tggttgtttt cttagggaaa
    17101 gcttctggtc caaaagcaaa cgggaagaac tatatcaaat caaaataatc ttttagcgaa
    17161 taaaaagggt gggttacggg aagtgttttt cgtctcgccc tttttgtttt aaaggcaata
    17221 aaaataaaaa gaaagaagga ataatcatgt ttaaacgcgt acatctgatc gtcatggatt
    17281 cagtaggaat tggagaagca ccagatgccg ataagttcgg tgatgttggt tctgatacat
    17341 taggacacat tgcgaaagaa gcaggattga acgttccaca tttagagcag cttggtttag
    17401 gaacgatccg tcctcttgaa ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga
    17461 aattagaaga agtttctgta gggaaagata cgatgacagg ccactgggaa atcatgggat
    17521 tgaatatcaa aactccattt cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac
    17581 aaatcgaaga attttctggc cgtaaagtcg tatgtaacaa accttatagc ggaacagctg
    17641 ttattgacga ttacggtgaa caccaaatga aaacaggcga tttaattgta tatacttctg
    17701 ccgatcctgt ccttcagatc gcggcacatg aagaaatcat tcctttagaa gaattatatc
    17761 ggatttgcca attcgttcgt gatataacaa aagatgatcc gtatatgatt ggccgaatca
    17821 ttgcacgtcc gtacgtgggt gaaccaggag acttcacacg aacaagcaat cgccatgatt
    17881 atgcgttgaa tccattcgga cacacagtcc ttgattcttt gaaagaagca ggaaaggacg
    17941 tgattgctgt tggaaagatc aacgatattt tcaacggaca aggaatcact gaatctgtcc
    18001 gcacgaaaag caatatggat ggcgttgacc agctgctgaa agttatgaaa aacgattttt
    18061 caggaatcag ctttaccaat ttagtagatt ttgatgcatt atacggtcat cgtcgggatg
    18121 tagtcggcta tgcgcatgcg attgaagaat ttgacttacg aattccagaa ttgcttgaag
    18181 cgatggaaga agacgacttg ctaatgatta cagccgatca cggaaacgat ccgacgttta
    18241 ctggtaccga tcatacgcga gaatatgtac cgttattggt ttacagtaaa aaaatgaaag
    18301 aacaaggggc attgcctcaa ggatattatt cagatatttc agcaacgatt gctgagaact
    18361 ttagtgtttc ttcgactgaa aatggcgaaa gcttcttgaa attattaaaa taatcttttt
    18421 aaataggaga aagcaaatga ccaagttaag cgagatgtta caagaaacga cccagtttat
    18481 ccttgataaa ggagtaaaag aaattgattt tggtttgatt ctaggttcag gattaggtga
    18541 attagcagat gaaatcgaag atgctgtaat gatttcttat gatgagatcc cgttctttcc
    18601 tgtttcgaca gtagttgggc atgcaggaaa gctagtttat gggtctctgg caggcaagaa
    18661 agtgttagcc atgcaaggga gattccacta ctatgaaggt cattccatgc aaacagtgac
    18721 ctatccagta cgtgtgatgg ccggactagg ggcccattca gtcattgtga ctaatgcatg
    18781 tggtggtgtc aacgaatcat ttgtaccagg agatttgatg ctgattaccg atcatatcaa
    18841 ttttactggt gaaaatcctt tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc
    18901 agatatgagt caagcttata cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa
    18961 actaggtttg gatcttaaag aaggcgtata tatgggctat tctggaccaa cttatgaaac
    19021 gcctgcggag atccgaatgt ctcggacaat aggagccgat gcagtaggga tgtcgactgt
    19081 tccagaagtc atcgtagcag ctcacagcgg attaaaagtg ttaggaatct cttgtattac
    19141 caacttagca gcaggtatgc aagcgaatct gagccatgaa gaagttgtag aaacaactca
    19201 gagagtcaaa caatctttta aagcattggt taaagaaaca ttgacattat tgtaatagga
    19261 ggaacaataa aatgagtgtg catattgaag cgaaagaagg cgagatcgcc gataagattt
    19321 tacttccggg agatcctttg cgagctaaat atattgcgga gacttttttg gaagatccag
    19381 tttgttacaa tcaggtacgc ggtatgttag gctttaccgg caaatataaa ggagaacgta
    19441 tttctgttca aggaacagga atgggaatgc catcagccac catctatgcc catgaattga
    19501 tccagtcgta tggtgtaaag aaattgattc gagtaggtac atgcggtgca ttgtcaaaag
    19561 acgtccatgt tcgtgattta gtactagcac aaggtgcagc gaccagttct tcgatgatcg
    19621 aaaaaaattt ccaagccttt cattttcctc caatcagtga tttcaatcta ttactgaaag
    19681 catatgagat agctaaagaa aagggctata cggttcatgt aggaaatgtt ctttcggaag
    19741 attctttcta caaagatgat ttgacagaga cgttccaatt agctgaactt ggcgttttag
    19801 gagtggaaat ggaagcagcg gctctttatt acttaggcgc gaaataccat gtgcagacat
    19861 taagtttgat gaccgttagc gaccatttga tcaccggtga agaaacaaca gctgcagaac
    19921 gtcaatctac gttcaatgaa atgatcgaag taggattgga aacagcgata gcagaataag
    19981 aaggaaatga aaaagctgcc ctgcattaag taataagaac atccaagcaa aaatagctcc
    20041 tcatgttttt gacttggctg gacttattat tgcaaatgta agcttttgaa caccgtttac
    20101 caagaggttg tgaatctgcc gtttaactct gagtcacaag gaacaatttc aaaaaacagc
    20161 ttttcatgtt tgatgaatat tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt
    20221 atacgaaaaa agggactgtg acaaatcgtg tcacagtccc ttttttatag tgttttttat
    20281 aggtagtatt ttttagcaac tgttaagtca tcatttaatt cataaacaag tggttgacct
    20341 gttggaattt caagatccat gatgtcttcg tcagagatac cttcgatatg tttagctagg
    20401 gcgcgtaatg agttaccatg tgcagctact aaaacagttt tgttgtctag taatgctgga
    20461 gcaatttcat cttgccagaa tggcaaagcg cgttctaatg tcactttcaa gttttctcca
    20521 ccaggaatat cgcgtttgtc taacattgcg taacgacggt catttgctgc agaaccttcg
    20581 tcagtttctt ccattaatgg aggaagagta tcgtaagaac gacgccagat gtgcacttgt
    20641 tcgtcgccgt atttttcagc tgtttctttt ttgttcaaac cttgtaattt accatagtga
    20701 cgttcgttta agcgccatga tttgatttgt ggtacccaaa gttgatctga atattctaag
    20761 atcaagttac atgttttgat tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg
    20821 attccagctt ctttgatttt gcgtccgcct tcttttgctt cttcgattcc ttctggtgct
    20881 agatttacat cagcccaacc agtaaattgg ttcaatgcat tccattcact aagaccgtga
    20941 cgagaaaaaa ctaattttgg catgtttagc tctcctttta agataggttt atatttctga
    21001 aattttcagt tctgttttat tgtacaattt tttggaagag atttccatcg aaaaattaga
    21061 taagattcta tgagaaaaca agtagagaat ataaaaaaac gaacagtgcc cacgcaactg
    21121 ttcgttttta ggtattagat gcataattgc tcgatttctt tttcaggtaa attgatatat
    21181 ttataacatg ttccaactga aacaccgcat tttgaagaaa taaattggat cgtttctttt
    21241 ttttcataat agagacgtcg gatttttttg atcgtttttg catcgatttt tggtcttccg
    21301 ccgatttttc cttttttacg agcttcatct aatccaacta atgtacgttc tttgatcaat
    21361 gcgcactcca tgtttgctag gttttccatt aattggaaat agatttttcc catttcgcgc
    21421 gtatcgattc cttcatcaag actaatcaag tgcagcccgc gatctttgaa taattgcgtg
    21481 agttctgtta attgccgcgt agatttgcct aatcgatgaa gttggcaaat gactagtgca
    21541 tcgccttgtt gcatgtcagc gatcactgac tccaaatgaa cagcagtaga atcattttgt
    21601 tccacttcat atgtctcatg gaaaatttct tgacaaccaa agtcagataa ttgatcaacc
    21661 tgagttgtta aatcaacgtc atttatggtt gtgcgtgcat aaccaataat tttcatggtg
    21721 attcctttct agcagtgatt ttctaactgg atataacagt aaaaatgtag cataaagtac
    21781 gtcaaaaaga tagttttttc gcaaattttc cgctgtcttt ttttattgga tgatcaagca
    21841 gtttttcttt atttacgacg gattgcgtca agaatctcaa cgcctttttc tgagccgaca
    21901 actactttag aaaccatgtt taggaaaagc ccatgttcta ctaccccaac aagttcatct
    21961 aaataggtag ctaagctttc tggctgttcg atgacttcta gatgcagatc aatgatgtaa
    22021 tgaccaccat ctgtggttaa gacttctccg ttttcgtctg tacgcaagac gggttggaat
    22081 cttttttctt caaaaatatg aagtagctgt tgggagccat aagggatcac ttcaactggg
    22141 agtgggaact tcccaagttt atcgactaat ttagaagaat caacgatcca aatcgtttct
    22201 tttgagtacg tagcgactat tttctcgaaa agcaaagcag cgccgccgcc tttgatccct
    22261 tgaaaatcag cactgatttc atcagcaccg tctattgtca aatcgactac ggggacttca
    22321 tcaatactct ttaacggtat tcctaatttt tccgcttgtt cttttgtcgc atttgaagta
    22381 gtaactcctg tgatacgtaa accttcttca cgcatacggc gaccaatctc ttctaccata
    22441 taataggcgg ttgaaccggt cccgagacct acgatcatcc cgtcctttac gaatttcgcg
    22501 gcttcaatgc cagctaattg ttttaaattc atctttgcgt ccaccttttt ttagtttgag
    22561 catcgtgctt tcctcactat tttagcgtga tttacggtaa aaagatacca ttctttttta
    22621 ttccaataaa gctttttcaa aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg
    22681 atattctaac aaaggtgctt tatctacagg tttctgtgaa gaaacgtgct gactgtttat
    22741 caaagcatat ttgataagga tgcagacatt gcatttattt tttatcacaa aaaaagaacc
    22801 acctggatgt gtggaactag acgcaaatcg tgggaaggag gaaacaagga atgcctacaa
    22861 ttaaccaatt agtacgtaaa cctcgtaaat caaaggtgga aaaatctaat tcacctgctt
    22921 tgaacaaagg atataacagc tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac
    22981 gtggggtagc aactcgtgtg ggtacaatga cacctaaaaa accgaactca gctttacgta
    23041 aatatgcacg tgttcgtttg tcaaacttaa ttgaagttac tgcttatatc ccagggattg
    23101 gtcataactt acaagaacac agtgttgtat tattacgcgg tggtcgtgta aaagacttac
    23161 caggggtacg ttaccatatc gttcgcggtg ctttagatac tgctggtgtt aacgaccgta
    23221 aacaaagccg ttctaaatac ggaactaaaa aacctaaagc ttaattttaa ttattaaaca
    23281 aatgaaactt gcttctacta tatagaagta attactatga acaatatttc ggaaggagga
    23341 gttacggatg ccacgtaaag gtcctgttgc aaaacgtgat gttttaccag atcctattta
    23401 taactcaaaa ttagtaactc gcttgatcaa ccgtgtaatg gttgacggaa aacgcgggat
    23461 tgcagctaac attatctata atgcctttga cttgatcaaa gaatctacag gtaacgatcc
    23521 attagaagtg tttgaacaag caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg
    23581 tcgtgttggg ggttcaaact accaagtacc agttgaagtt cgccctgaac gccgcgttac
    23641 tctaggtctt cgttgggtag taaactacgc tcgcttgcgc ggtgaacaca caatggaaga
    23701 acgcttagca aaagaaatca tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg
    23761 tgaagacact cataaaatgg ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga
    23821 ttctctctgt ttggtcggct tttataagtc gacctcagat agtgtcctta tttttagcaa
    23881 ctagatagta aagagaggag aaacaaggag aatgaccaga gaattttctt tacaaaacac
    23941 tcgtaatatc ggtatcatgg cccacatcga tgctggtaaa acgactgcta ctgaacgtat
    24001 cttgtactat actggtaaaa tccataaaat tggtgaaact catgaaggag cttcacaaat
    24061 ggactggatg gaacaagaac aagaacgtgg tattacgatc acttctgctg ctactacagc
    24121 acaatggaag ggtcaccgaa tcaacatcat cgacacacca ggacacgtag acttcacagt
    24181 tgaagttgaa cgttcattgc gtgtacttga tggtgctgta actgttcttg atgcacaatc
    24241 aggtgttgaa cctcaaactg aaacagtttg gcgtcaagca acaacttatg gtgtacctcg
    24301 tatcgtattt gcaaacaaaa tggacaagat cggtgctgac ttcttatatt cagtatcaac
    24361 tttacatgac cgtttgcaag caaacgctca cccaatccaa ttaccaattg gtgcggaaga
    24421 cgacttcaca ggaattatcg accttgtaac aatgaaggct gaaatgtata caaacgatct
    24481 tggaacagag atcgaagaaa cagaaattcc tgaagaatac cgcgaattag ctgaagaatg
    24541 gcgcgaaaaa ttggttgaag ctgtagctga aactgatgaa gaacttactt tgaaatactt
    24601 agaaggtgaa gaaatcactg aagctgagtt aaaagaaggt atccgccgcg caacagttaa
    24661 cgtagaattc tacccagtac tatgtggttc agcatttaag aacaaagggg ttcaattact
    24721 attggacgct gttcttgatt acttgccatc accgttagat atcccagcta tcaaagggat
    24781 tgaccctaaa actgatgaag aagtagaacg tcctgcagac gacagcgctc ctttctcagc
    24841 tttagcattt aaagttatga ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta
    24901 ctctggtgtt ttacaatcag gttcatacgt acaaaacgca acaaaaggca aacgtgaacg
    24961 tgttggtcgt atcctacaga tgcatgccaa ctctcgttca gaaatctctg aagtttacgc
    25021 tggagatatc gctgctgctg ttggtttgaa agacacaact actggggaca cactatgtga
    25081 tgaaaagagt ctagttattc ttgaatccat ggaattccca gaaccagtta tccaagtcgc
    25141 tattgaacct aaatcaaaag ctgaccaaga taaaatgggt gtggctttac aaaaactatc
    25201 tgaagaagat cctactttcc gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc
    25261 tggtatgggt gaacttcact tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt
    25321 tgaagctaac gtaggtgcgc ctcaagtatc ttaccgtgaa actttccgtg ctggcactca
    25381 agccgaaggt aagtttgtac gtcagtctgg tggtaaaggt caatacggtc acgtatggat
    25441 tgaatttaca ccaaatgaag aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg
    25501 ggttgttcct cgtgaataca ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa
    25561 tggtgtctta gctggctatc cattagtaga tatcaaagct aaactttacg atggttcata
    25621 ccatgatgtc gattcaaatg aaactgcctt ccgtgttgct gcttctatgg cattacgtgc
    25681 agcagctaag aaagctaatc cagtaatcct tgaaccaatc atggctgtag aagttgttat
    25741 ccctgaagat tatttaggtg atgttatggg tcacgtaact gctcgtcgtg gacgtgttga
    25801 aggtatggaa gctcgcgctg gcggccaaca agttgttcgt gcgatggttc cacttgctga
    25861 aatgtttggt tatgctacaa ctcttcgttc tgctactcaa ggtcgtggta catttacaat
    25921 gacatttgac cactacgaag atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa
    25981 cggcggaaaa gctgaataat ttcttaaaac cggatagtcc tgcaaagaga ttatccaaag
    26041 agactatatt tttaatatag tttcgtgtaa aataattatc gatgatatgg gcatgtggtg
    26101 taccttgtgt acatcatata cctatcagac aaataaaaaa tatattgtat ttttaggagg
    26161 acattttaaa atggcaaaag aaaaatttga ccgttctaaa ccacacgtta acattggtac
    26221 tatcggacac gttgaccatg gtaaaactac attaacagct gcaatcacaa ctgtactatc
    26281 taagaaaaac ggcggccaag ctatggctta cgatcaaatc gatggtgctc cagaagaacg
    26341 cgaacgcgga atcacaatct caacagctca cgttgagtat gaaacagata cacgccacta
    26401 cgctcacgtt gactgcccag gacacgcgga ttacgttaaa aacatgatca ctggtgctgc
    26461 tcaaatggac ggagctatct tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg
    26521 tgaacacatc ctattgtctc gtcaagttgg tgttccttac atcgttgtat tcttgaacaa
    26581 agtagacatg gttgatgacg aagaattact agaattagtt gaaatggaag ttcgtgacct
    26641 attaacagaa tacgaattcc ctggtgacga tgttcctgta gttgctggat cagctttgaa
    26701 agctctagaa ggcgacgctt catacgaaga aaaaattctt gaattaatgg ctgcagttga
    26761 cgaatacatc ccaactccag aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga
    26821 cgtgttctca attactggac gtggtactgt tgctacaggt cgtgttgaac gtggacaagt
    26881 tcgcgttggt gacgaagttg aagttgttgg tattgctgaa gaaacttcaa aaacaacagt
    26941 tactggtgtt gaaatgttcc gtaaattgtt agactacgct gaagctggag acaacattgg
    27001 tgctttacta cgtggtgttg cacgtgaaga catccaacgt ggacaagttt tagctaaacc
    27061 aggtacaatc acacctcata caaaattctc tgcagaagta tacgtgttga caaaagaaga
    27121 aggtggacgt catactccat tcttcactaa ctaccgtcca caattctact tccgtacaac
    27181 tgacgtaaca ggtgttgttg aattaccaga aggaactgaa atggttatgc ctggcgacaa
    27241 cgtaacaatg gaagttgaat taatccaccc aatcgctatc gaaaacggta ctaaattctc
    27301 aatccgtgaa ggcggacgta cagttggtgc cggtgtcgtt acagaaatca aagcttaatt
    27361 cacttaagtt ttaaagtaaa gcaacattta ttcggacgta atcgctcgga acgaaggtct
    27421 taggatcttt gttccgagct tttttattta ttgcaggatc aggaaaaaag acaggagtat
    27481 tataaataga agaaacacga aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa
    27541 acaaaatgtt ttttacaaga aaaagccttg ctttgtgagt ttatatatat tataatgaca
    27601 aaggtgcgtt tactttagtg aattgcacag acgtaatcag aatgatttcg tcggctaaga
    27661 aacgagaggt tgcgacacgc ccggacgctt tgccacgaac gagcgtgacg gaaattttcg
    27721 tggagctatg tctacttttc aaaataggcg aaggagggaa caagatggca aaacaaaaaa
    27781 ttcgtatccg tttaaaagcg tatgaacacc gtattttaga tcaatcagcg gataaaatcg
    27841 tggaaactgc aaaaagaact ggagctagcg tgtctggtcc aattccatta ccaactgaac
    27901 gcagtcttta cacaatcatc cgtgcgactc ataaatacaa agattcacgc gaacaattcg
    27961 aaatgcgtac acacaaacgt cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg
    28021 ctttgatgaa gctagactta ccatctggtg taaacatcga aatcaaatta taaaactaaa
    28081 acaaaaataa tggaggtgta ctcatgacca aaggaatctt agggaaaaaa gtgggaatga
    28141 cacaaatctt cactgaatct ggtgaattaa ttccagtaac tgttgtagaa gctactccaa
    28201 acgtagtact acaagtgaaa acaatggaaa cggatggcta cgaagctatt caagttggtt
    28261 accaagatat gcgtgaagtt ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa
    28321 acacggctcc taagcgcttc attcgtgaat tcaaaaatgt tgagctagga gaatatgaag
    28381 taggaaaaga aattacagta gatgttttcc aagcaggaga cattattgat gtaacaggta
    28441 ctacgaaagg taaaggattc caaggggtta tcaaacgcca cggacaaagc cgcggaccaa
    28501 tggctcacgg ttctcgttac catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc
    28561 gtgtatttaa aaacaaacgt cttgctggcc gtatgggtgg caaccgcgta actatccaaa
    28621 acttggaagt tgtacgtgtc gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc
    28681 ctggagctaa aaaatcttta attactatca aatcagctgt gaaagctaaa taattgtagg
    28741 aagaaaggag gaactaagga atgccgaatg tagcattatt caaacaagat ggaagccaaa
    28801 acggtgaaat cacacttaac gaagaaatct tcggaattga acctaatgaa agtgttgttt
    28861 acgatgcaat cgtaatgcaa cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc
    28921 gtagcgctgt tcgcggcggc ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac
    28981 gtcaaggatc tatccgctca ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac
    29041 cacgttctta cagctacaaa cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat
    29101 tgtcagaaaa agttgctgaa aacaacttgg tagcaatcga aggcttgaac ttcgacgcac
    29161 caaaaacaaa agaattcaaa caagttcttg ctaacttatc tatcgactct aaagtattgg
    29221 ttgttctaga aaaaggtaac gactttgctg ctttatcagc acgtaactta ccaaacgttt
    29281 ctgtagtaac ttctgataat gtgagtgtgc tagacgttgt atcaaataca aaagtgttag
    29341 caacacaaac tgctcttact caaattgagg aggtgcttgc ataatgaact tactagacgt
    29401 aatcaaacgc ccagtgatca ctgaaaaatc aatgcttgcc atggatgaca agaaatacac
    29461 ttttgaagtg gacactcgcg caaacaaaac tttagtaaaa caagcggtag aagctgcttt
    29521 tgacgttaaa gtcaaaaacg tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg
    29581 caaatatgca ggatatacaa aaaaacgtcg caaagcgatc gttacattga ctgaagattc
    29641 aaaagaaatc caattattcg aagctgctga ataagcacta atagataact cattaaatta
    29701 ggagggaaaa acgtggcgat taaaaagtac aaacctacct caaatggtcg tcgtaacatg
    29761 acaggttctg attttgctga aatcacaaca tcaacacctg aaaaatcatt gttacaacca
    29821 ttgaaaaacc atgctggacg taacaacaac ggccgcatca ctgtacgtca tcaaggtggc
    29881 ggacacaaac gccaatatcg cgtgatcgac ttcaaacgta acaaagataa cgttgttgca
    29941 actgttcaaa cgatcgaata tgatccaaac cgttcagcta acatcgcgtt agtacactat
    30001 gaagatggag tgaaagctta catcttagca cctaaaggac tagaagtggg tatgaaactt
    30061 gtttcaggtc cagaagcaga tatcaaagtc ggaaatgctc ttccattaga aaacattcca
    30121 gttggtactg ttatccacaa cattgaaatg aaacctggta aaggtggaca attgatccgt
    30181 tctgctggta caagtgctca agtacttggt aaagaaggca aatacgtatt gatccgcttg
    30241 aactctggtg aagttcgtat gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt
    30301 aacgaacaac acgaattaat caacatcggt aaagctggac gctctcgttg gatgcgtaaa
    30361 cgcccaactg tacgtggtag cgtaatgaac cctaatgatc acccacacgg tggtggtgaa
    30421 ggtaaagctc ctatcggacg taaagcacca gtttctcctt ggggtcaacc agctcttggt
    30481 tacaaaactc gtaacaaaaa agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat
    30541 aaataattac tttgaccatg tgtcgcacat tttgagagga ggttcaccat gggtcgtagt
    30601 ttaaagaaag gaccttttgt cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg
    30661 gttgaaaaga aaaaagttat caaaacttgg tcacgccgtt caacagtttt cccaagtttt
    30721 gttggatata ccatcgcagt ttatgatgga cgtaaacatg taccagttta catccaagaa
    30781 gacatggtag gacacaaatt aggtgaattt gcacctacaa gaacttatcg tggacatgct
    30841 gctgacgata agaaaacgaa acgctaattt gaggggagga taaatccaaa tggcagaaca
    30901 aatcacatca gctaaagcaa ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt
    30961 agtaattgac cttatcagag gtaaaagtgt cgcagatgca atttcaatct tgaaattcac
    31021 accaaacaaa tctgctggaa tcattgaaaa agtattgatg tcagcagttg cgaatgcaga
    31081 aaataacttt gacttagatg ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg
    31141 acctacaatg aaacgtttcc gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg
    31201 tacaagtcat atcacggtag tcgtatcaga aaaataagga gggataatca gtgggtcaaa
    31261 aagtacatcc aattggaatg cgtgtaggca tcatccgcga ctgggatgct aaatggtatg
    31321 ctgaaaaaga gtatgctgag ttcttacacg aagatttaag aatccgtaaa tttatttcaa
    31381 ctaaacttgc tgatgctgct gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa
    31441 acatttcaat tcacacagct aaaccaggta tggttattgg taaaggcggt tctgaagtcg
    31501 aaagcctaag aaaagaatta aacaaattaa ctggtaaaag aatccatatc aatatcgtgg
    31561 aaatcaaaaa accagatcta gatgcaaaat tagtaggtga aggaatcgca cgtcaattag
    31621 aaaaccgtgt tgctttccgt cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg
    31681 gcgctcaagg gatcaaaact caagtatctg gtcgtctaaa cggagcagat atcgctcgtt
    31741 cagaaggata ctcagaagga actgttcctc ttcatacttt acgtgcggac attgattacg
    31801 catgggaaga agcagataca acatacggaa aactaggagt taaagtgtgg atctatcgtg
    31861 gagaaattct tccaactaaa aaaaacactg agaaaggagg gaaataatca tgttagtacc
    31921 taaacgtgta aaacaccgtc gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg
    31981 aaaagaagta gcattcggtg aatacggttt gcaagctgtt gattcacatt ggatcacaaa
    32041 ccgccaaatc gaagctgctc gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt
    32101 atggattaaa attttccctc acaaatctta tactgccaaa gcaattgggg tacgtatggg
    32161 ttctggtaaa ggggcacctg aaggatgggt tgcaccagta aaacgtggta aaatcatgtt
    32221 tgaaatcgca ggcgttcctg aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa
    32281 attaccaatg aaaactaaga tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg
    32341 ttaaagaaat cagagaatta accactgccg aaatgcttga tcaagaaaaa caattaaaag
    32401 aagaattgtt taatcttaga ttccaattag caacaggtca attagaaaac actgcacgta
    32461 ttaaagaagt acgtaaatcg attgcacgca tcaaaactgt gttgcgtgaa caagctaagt
    32521 aattatggaa ggaggccatt aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc
    32581 aaggtcgcgt ggtttcagac aaaatggata aaacaattac cgttgctgtc gaaacaaaga
    32641 aaaaccaccc tatctatggc aaacgcatga actattctaa gaaatacaaa gtgcatgatg
    32701 aaaacaacac agcaaaagtt ggcgacatcg tgagaattat ggaaactcgt ccattatcag
    32761 ctacaaaacg tttccgtttg ttggaagtag tcgaagaagc agttattatc taataacacg
    32821 agattttcct atatagattg aatgcaaaat ctgaaaggag gatacacaac gtgatccaag
    32881 cagaaagtcg tttaaaagtc gctgataact caggcgcacg tgaaattttg acgatcaaag
    32941 tccttggtgg atctggtcgt aaaactgcta acatcggtga tgtgatcgtt gctacggtta
    33001 aacaagcaac gccaggtggg gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc
    33061 gaacaaaatc aggagctcgt cgtacagacg gttcatatat taaatttgat gaaaatgctg
    33121 cagtgattat tcgtgatgat aaaagccctc gtggaacacg tatctttggg ccagttgcac
    33181 gtgaactacg tgaaaacaac ttcatgaaga tcgtttcact agcaccagaa gttttataat
    33241 cttgagacac ttatcaaagg aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag
    33301 ttaaagttat cactggaaaa gacaaaaaca aagaaggcgt tgtactagca gcgttcccga
    33361 aaaaagataa agttattgtc gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc
    33421 aagctgctcc tcaaggtgga attgttgaga tggaagcgcc aattcatgtt tctaacgtga
    33481 tggtggttga ctctactggt gtagctggcc gagttggcta caaagaagta gacggcaaaa
    33541 aagtccgtgt ttctaaaaaa accggtgaag ttttagataa ataaaagttt aaggaaggag
    33601 ggtcttactg aatgaaccgc ctaaaagaaa aatatcttaa agaagtaact ccatcattga
    33661 tggaaaaatt taactatagc tctgttatgc aaacacctaa agttgaaaaa atcgtcatca
    33721 acatgggtgt tggtgatgcg gtatcaaacg ctaaaaactt agataaagca gttgaagaat
    33781 taactttgat cactggtcaa aaaccaatga ttacaaaagc taaaaaatca atcgctggat
    33841 tccgtttacg tgaaggaatg ccaattggtg caaaagttac attgcgcgga gaaagaatgt
    33901 acgaattctt agacaaattg gtttctgttt cactaccacg tgtacgtgac ttccatggtg
    33961 taagtaaaaa agcctttgac ggtcgtggaa actatacttt aggtattaaa gaacaattga
    34021 tcttcccaga agtagattat gatttagtag ataaagtacg tggtatggac atcgttattg
    34081 taacaacagc aaactctgat gaagaatctc gtgagttgtt gacacaatta ggcatgccat
    34141 tccaaaaata attaaaggag gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca
    34201 aacgtcctgc aaaattttca acacaagctt atactcgttg cgaacgttgc ggacgtccac
    34261 attcagttta tcgtaagttc catctttgcc gtatttgctt ccgcgaactt gcctataaag
    34321 gtcaaattcc cggcgtgaag aaagctagct ggtaaaaagt aaattcgcat aagaaggagg
    34381 taaaagatca atggtcatga cagatccaat tgcagatttt ctaactcgta tccgtaacgc
    34441 aaacatggtt aaacatgagt ctttagaagt acctgcatca aaaattaaac gtgacatcgc
    34501 tgaaatcttg aaacgtgaag gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca
    34561 aggcgtgatc cgtgttttcc taaaatttgg taaaaacggc gaacgtgtta tcacaaactt
    34621 aaaacgtatc tctaaaccag gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt
    34681 attgaacggt ttaggtatcg caatcatttc gacttctgaa ggtgttgtca ctgataaaga
    34741 agctcgtgaa aaaaatatcg gcggcgaagt tatcgcttac gtatggtaat caagtagaat
    34801 ctacaaggag gtgtctttaa gtgagccgta tcggaaataa agtcgttgtt atccctgaag
    34861 gagtaacagt tactcaagac ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga
    34921 ctcgtacttt ttcagcagat atcaaaatga atatcgaagg aaacgaagta actttcactc
    34981 gtccaaacga cagtaaagaa atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca
    35041 acatggttgt aggtgtcagc gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt
    35101 accgtgcaca attacaagga tcaaaacttg tattgaacgt tggttactca catccagtag
    35161 aaatcacacc accagctgga gtaactgttg aagtaccagc taacacacaa gtaattgtta
    35221 aaggtgcgaa caaagaagaa gttggcgaat tagctgcaaa cattcgtgga actcgtcctc
    35281 cagaacctta taaaggtaaa ggtattcgtt atgttggcga atatgtacgc cgtaaagaag
    35341 gtaaaactgg taaataatcg tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc
    35401 atttggctga agtttacctt caaaaaaata catttttact tgtgccgcag cttctatttt
    35461 tcgaaacgta tttactgctt gaaatagtag tacaataaaa ataaagaggt gacaattgtg
    35521 attacaaaac cagataagaa caaaacacgt caaaagagac atcgtcgtgt acgtaacaat
    35581 atctctggta ctgctgagcg cccacgcttg aacgtttttc gttctaacaa aaacatctac
    35641 gctcaaatta ttgatgacgt agcgggtgta acgctagcaa gtgcctctac cttggataaa
    35701 gaaatttcag gtggaacaaa aacagaagca gcacaagctg tcggtaaact agttgctgaa
    35761 cgtgcagctg aaaaaggcat taaagtagta gtctttgacc gtggtggata cctttaccat
    35821 ggccgtgtgc aagctttagc tgaagctgct cgcgaaaatg gactagaatt ttagaagaag
    35881 gaggaatacc attcatggtt tatatcgatc caaaacattt ggaattagaa gaccgcgttg
    35941 ttgcaattaa ccgtgtaaca aaagttgtta aaggtggacg tcgtctacgt tttgctgctt
    36001 tagttgttgt cggtgacaaa aacggacacg tagggtttgg tacaggtaaa gctcaagaag
    36061 ttcctgaagc aatccgtaaa gcaattgaag atgcgaagaa aaacttagtt gaagtaccta
    36121 tggttggttc aacaatccca cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa
    36181 tgaaacctgc tgtagaaggt tctggggttg ctgctggtgg accagttcgt gccgtattag
    36241 aattagctgg ggtagcagat atcacttcta aatctttagg ctcaaataca ccaatcaacg
    36301 ttgttcgcgc aacagttgaa ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac
    36361 ttcgcggcaa atcagttgaa gaattaatcg gataaggagg acaacaataa tggctgaatt
    36421 aaaagttact ttaaaacgca gtattatcgg acgtcctcaa aaccaacgcg atacagttaa
    36481 agcgttaggt cttacaaaga ttaacagttc agtggttaag ccttctaatg aagcaattaa
    36541 aggcatgatc aatactgttt ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat
    36601 cataactaga acattaagga ggtgccaaac ctatgaaact tcatgaatta aaacctgctg
    36661 aaggttcacg ccaagtacgt aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa
    36721 ctgccggacg cggacaaaaa ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat
    36781 ttgaaggggg acaaacacca ttgttccgtc gtttaccaaa acgaggattc acaaacgtga
    36841 accgtaaaga ctatgcagtc attaatcttg atgtattgaa ccgctttgaa gacggagctg
    36901 aagtaactcc tgttgcttta gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca
    36961 aagtattagc taatggggaa ctaaacaaga aattaactgt gaaagcagca aaattctcta
    37021 aagcagctca agaagcaatt gaagcggctg gtggatcaat cgaggtgatc taatgtttag
    37081 actattaaaa gatgctttta aagtaaaaga cattagatcg aaaatcctat ttactgtatt
    37141 tgtgttgttt gtatttcgcc taggtgcaca tattactgta ccaggcgtaa atgctgcttc
    37201 attgcaaaac ctttcttctt tgccattttt caacatgttg aacttggtaa gtggtagtgc
    37261 gatgcaaaac ttttccattt tctcaatggg ggtttcacca tacattactg cttctatcgt
    37321 cattcagctt cttcaaatgg acatcgtgcc tcgatttgtt gaatggtcga aacaagggga
    37381 agttggtcgt aaaaaactga atcaagcgac tcgatatctg acattagtat tggcatttat
    37441 ccaatctgtc ggtatcactg ctggattcaa tcaatacgct caattaggtt tcgtgaataa
    37501 tcctaacgta gcaacttacg taacgatcgg tttgattcta acagccggta cgatgtttgt
    37561 tacttggcta ggagaacaga tcacagaaaa aggtatcgga aatggcgtat caatgatcat
    37621 ttttgcaggt atcatttcta gattgcctga aagtatcaga gaaatttatg aagattactt
    37681 tataaatatt gatccttcag acatatggaa atctgctatt tttgtggcca ttttactagt
    37741 agcggtcttg ttgatcgtga cattcgtaac atttttccaa caagctgaac gcaagatccc
    37801 gatccaatat acgaaacgcg tagctggcgc gcctacaagc agctatctgc cgttgaaagt
    37861 aaatgcagct ggagttattc cagttatctt tgctagttcg tttattgcta cgccaaatgc
    37921 tgttttacaa gcattgagcg gtagattcgg ttctgaaaac tggtacaaag tgatgatgga
    37981 gatctttaac tataatacag cacctggtgc tacgatttat acgattctga tcgttgcctt
    38041 cactttcttc tacgcctttg ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa
    38101 gcaaggaagt tatataccaa gcgtacgccc tggtaagggg actgaagagt atgtctcaag
    38161 attactgatg cgtttaagta cagttggtgc agtcttcctt ggtttagtgg cattgcttcc
    38221 aatcattgct caaatggttt gggatcttcc acaatcaatc ggattaggcg gtacaagctt
    38281 actgatcgtt atcggtgtag ctttagaaac tgcgaaacag ttagaaggct taatgctgaa
    38341 acgtaaatat gttggattca ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg
    38401 ttctcacaca tttattagga gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg
    38461 caggtaaagg aacacaagct gaaaaaatca ttgatgctta tggtattccg cacatctcaa
    38521 caggagatat gttccgcgca gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt
    38581 cttatatgga taaaggtgca ttagttcctg atgaagtaac aaatggaatc gtgaaagagc
    38641 gactagctga accagataca gagaaaggct tcttattaga tggctttcca cgtacgctag
    38701 atcaagcaaa agctctggac gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca
    38761 tcgatatcca tgttggtgaa gaaatcttgg tcgaacgtct agccggccga tttatctgca
    38821 gcaattgtgg agcaacttac cataaaattt tcaaccctac aaaagttgaa gacacatgtg
    38881 atcgttgcgg tgggcatgaa ttctatcaaa gagaagatga taaacctgag acggttaaaa
    38941 atcgtctggc tatcaacatc aaaaatagtg aaccaatctt ggcttattat aaagaacaag
    39001 gtttgctaaa cactattgat ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa
    39061 aaatcattga aaaatagcac gattgcccgt agccgacttg cattatgcag ttactcttgt
    39121 caagttcaat aaattatgat agaatatgtc agtccgactt ctagagaatc ctctagatga
    39181 tccattttga gaattttgag gtgggaactg cagtttccgc cattttatcg tgtgaaaatg
    39241 cgaaacaagt acaaggaggt actgtgcgtg gcaaaagaag atatgattga agtcgaaggt
    39301 acagtcgtcg aaactttgcc gaatgcaatg tttaaagttg aactagaaaa cggacaccaa
    39361 gttcttgcta ctgtttcagg taaaattcgt atgcattaca tccgtatctt acctggtgac
    39421 aaagtaacgg ttgaattgtc tccatacgat cttaaccgtg gccgtattac ttatcgcttt
    39481 aaataattgt actccgtaaa actacaggag gtattatcat gaaagtaaga ccatcagtaa
    39541 aaccaatgtg cgaacattgt aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc
    39601 cagcaaatcc aaaacataaa caacgtcaag gataattgga ggtgctataa atatggctcg
    39661 tattgcagga gtagatattc ctcgtgataa acgtgtagta gtttcactta cttatatcta
    39721 tggtattggt aacactactg ctaaaaagat cttagctgat gctggtgtat ctgaagatgt
    39781 tcgcgttcgt gaattaacga atgaacaaac agatgctatc cgtgcagaag tagataaatt
    39841 aaaagttgaa ggggatcttc gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat
    39901 cggttcttac cgtggaatcc gtcatcgccg tggattgcct gttcgtggac aaaacacgaa
    39961 aaacaatgca cgtactcgta aaggcccagc tcgcgctatc gctggcaaga aaaaataatt
    40021 cttaagtgaa ggaggttaaa acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt
    40081 gaagaagaat atagaagcag gtattgcaca tatccattct acattcaata acactatcat
    40141 catgatcact gatactcacg gaaatgcatt agcgtggtca tcagctggtt cattaggttt
    40201 caaaggaagc aaaaaatcaa ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa
    40261 agcagcaatg gaacacggat taaagactgt tgaagtaaca gttaaaggac ctggttctgg
    40321 acgtgaagca gcaattcgtt cattacaagc tacaggttta gaagtgactg caattcgtga
    40381 cgtgactcca gttcctcata atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt
    40441 gccgctcatt ttgagtttta tcagtaacgt cattgaacaa gacacttttc gttttgaaag
    40501 gggtaaagat aagaatgatt gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa
    40561 aagattatgg caagttcatc gttgaacctc ttgaaagagg gtatgggact actttaggga
    40621 attccctacg tcgtatttta ttatcttctt tgccaggtgc tgccatcact aatattcaaa
    40681 tcgatggtgt actgcatgaa ttctctacca tcaaaggtgt tagagaagac gtcactcaaa
    40741 tcatcttgaa cattaaaggc ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg
    40801 aaatcgatat taccggacct gctacagtaa ctgctggcga tattatcgtt gatagtgatg
    40861 tagaaattct taacaaagat atgtacatct gtagtgtctc tgaaggagct acgttccatg
    40921 ctcgcttaac agtgaaacct ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag
    40981 atatgccaat tggcgttctt ccagtcgatt caatctacac acctgttcgt cgtgtgaact
    41041 atcaagttga gaacacacgt gttggacacc gtgaagattt tgataaattg acgatggaga
    41101 tttggacaga tggttcaatt gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga
    41161 ctgaacattt ggatattttt gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg
    41221 tcgaaaaaga agaaacacaa aaagaaaaaa tgttggaaat gacgatcgaa gagttagact
    41281 tgtctgttcg ctcttacaat tgtttgaaac gtgcaggaat caatactgta caagaactta
    41341 caaataaatc tgagcctgaa atgatcaaag tccgtaactt aggacgtaaa tcattagaag
    41401 aagttaaagc aaaattgcac gacttaggtt taggtttacg taaagaagat taaaaccttt
    41461 tacgaaggag ggaaaccaac gtgagttatc gtaaactagg acgcacatct agccaacgta
    41521 aagcgatgtt gcgtgattta acaactgatt tattaatcaa cgaacgtatt gtgactactg
    41581 aagctcgtgc gaaagaagtt cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg
    41641 gagatttgca tgcacgtcgt caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc
    41701 gtgaagaaaa tgaggacatc gtcattgaat cagctttaca aaaactgttc aacgatattg
    41761 cacctcgtta cgcagaacgt caaggcggat acacacgcat tttgaagaca gaaccaagac
    41821 gcggtgatgg tgcaccaatg gttgtcttag aacttgtcta aaatcacact aataacatca
    41881 ctttatagat gattctttta gagtgttatg atgttggaag atatttttcc gagtctagct
    41941 cagcgctgct cctcaagcga atatcgcttg gggagtaagc actcatcata aagtgttttt
    42001 ttataccttc aattacctgt catgacagat atgtttgaag gcataaaaaa acattttaag
    42061 cgagaaataa ttgaaaattt tgtctttttt tgatagtgtt agtaggaact ttaggatgaa
    42121 gaaggagtaa tcatggagcc aattattgaa ttagaaaaga taaattataa ataccagcca
    42181 gatgatcttc gccccgcatt gaaagatgtc tcctttacaa ttgataaagg tgagtggatc
    42241 gctattattg gacacaatgg ttcaggaaaa tcgacacttg ccaaaacgat caatggattg
    42301 cttctgccag aatcagggat cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt
    42361 tggacgatcc gacagatggt tggaatgatt tttcaaaatc cagacaatca gtttgttggt
    42421 tcgacagtag aagatgacgt tgcttttggt cttgaaaatc aagggattcc cagagaagaa
    42481 atgctagtgc gtgtgaaaga tgcacttgag aaagttagaa tggctgagtt tgcttcacgc
    42541 gagcctgctc gtttatcagg agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc
    42601 ttacgacctg atatcattat tttagatgaa gcaaccagta tgctagatcc agaaggtcgt
    42661 gaagaagtga tctctacgat caagaagatc aaagaagaaa gtcaattgac cgttatatct
    42721 atcactcatg atattgatga agcagctaat gccaatcgga tactagtgat gagacaaggc
    42781 gaacttgtcc gtgaaggtac accaaaagaa attttttctg ctggtcctga actgatcgac
    42841 ttaggtctag atctaccatt tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt
    42901 gatgtgccca gtgaatatat gacagaagaa aggatggtgg attggttatg gacatccgtt
    42961 ttgaacaagt agacttcact tatcagccga atacaccatt tgaacaaaga gccttatttg
    43021 acatcaatat gacgatcaaa gaaaacagtt atactgcatt agtagggcat accggaagcg
    43081 ggaaatcaac cttacttcag catttgaatg cgcttgttaa gccaacaagc ggtacagtcc
    43141 atattggaga acgagatatt cagccggata cggataataa gaatttaaag cccatccgaa
    43201 aaaaagttgg catcgtcttt cagtttccag aagcacagct attcgaagaa acggtagcaa
    43261 aagacattgc tttcggtcct aaaaactttg gtgtcagcga agaagaagca ctagtcctag
    43321 caaaagaaac attagaacaa gttgggctgg atgaaagcta tttggaacgt tcgccatttg
    43381 aactttcagg agggcaaatg cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg
    43441 aagtacttgt attggatgaa ccaacggcag gacttgatcc ccagggcaga aaagaaatga
    43501 tggagatgtt ttggcgtctt cataaggaac agcagatcac tattgtatta gttacgcatt
    43561 taatggatga tgtagccaat tttgcagatt atgtctatgt gttggaaaag ggacgaatcg
    43621 ttaatagcgg ggagccgcaa gaggtctttc aaaacattga atggctaaaa gaaaaacaac
    43681 taggcgtacc tactgccacc gagtttgcag aagaattaat ggctaaaggg atgaactttg
    43741 ctaccttgcc tttgacagct gaagaattag cagacgctat cgtccattat gctggaggaa
    43801 atgcccatga tgaataaatt gatatttggt cgttatatgc ctggagattc cttgatccat
    43861 cgattggatc cccgagctaa attattagcc agtttttatt ttatcggaat cattttttta
    43921 gcaaataatt gggaaacgta ccttttgttg gcatttttca cattactttc tgtcttcttg
    43981 tcaaaagtag atttgaaatt ttttatccga ggcattcgcc cattgatctg gctaatcctc
    44041 ttcactgtag ctttgcaggt cttgtttaca agtggcggag aaacttattg gagttggggt
    44101 gttttccata tcactgaatt tggtgtgatg aacggcctct ttattttctg tcgattcgtg
    44161 ttgatcattt ttatgtcgac tctactcacg cttactacac cgcctttgga attatcggat
    44221 gcgattgagt acttgcttcg tccattgaaa gccgtacggt tcccagtcca cgaaatttca
    44281 ttgatgctgt cgattgctct tcgttttgtg cctacattga tggacgaaac agaaaagatc
    44341 atgaacgctc agcgcgctcg cggtgttgat tttggtgaag gaaacctagt tcagaaaatg
    44401 aaagcagtca ttcctttgct gattccttta tttgtcagca gcttcaaccg tgcggaagat
    44461 cttgcgactg ctatggaagc aagaggctac aaaggtgggg aaggaagaac gaaatatcgt
    44521 gtattgcatt ggcataatag agacacgatg gttatcattg cttttgtatt attaactgtg
    44581 ctactgatct ttttaagagg ataaacgttc agtgggccgc tatctattct tttcttgctg
    44641 atctgtttgc aagaaaggat gcaagcagtg tatgacaaga aatattaggg tctgtggcag
    44701 actaaggtca caggcctctt tcttttatct gaaaagaaag aacgaaaaaa ttttatagaa
    44761 aaatcattat tccagataac cttttaggaa caaaggagag aaaaacatgg tacgttacaa
    44821 agcaatcatt gcgtacgacg gaacaaattt taatggattc caaaaacagc caaatgggcg
    44881 tactgttcaa gaagaagtag aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac
    44941 ggtctttggt tcgggaagga cagatgccgg agtacatgcg atcgggcaag taatccattt
    45001 tgattatcca gaggagcgtc cgttagaacg aatgcgtttc gcgctagata cccagtcacc
    45061 agaggatatt gctgtaagac aagtagaaat tgtttcagaa gacttccacg caagatattt
    45121 ggtcaaagaa aagacgtatc aatttcgagt ggatataggg aaaccacgca gtccttttcg
    45181 acgccattat gcaagttatt ttccttaccc acttgatttg gaaaaaattc agcgggcgct
    45241 tcctgattta ttagggactc acgatttcac ctctttttgt gcatcaggaa gctcgataga
    45301 ggataaaata cgtacgatct acgaggctaa gatggaggta aatgaaacaa aagacgaatt
    45361 gttgtttaca tttaggggaa atggcttctt gtataagatg atacggatct tagtaggaac
    45421 gcttttgaaa attggcaatg gccgattgcc tgaagatagt atcccagata ttattgcgaa
    45481 aaaagatcgt aatgcagcag gacctacagc acaccctgaa ggactttatt tgtatgaagt
    45541 gaaatatgaa taagaggtta tgatagaagt ggtcagcttc aagaaataag aaggtattta
    45601 cgaaaattac ttctcaaaat tttgcaaaat ttcggcttat tttcgaagaa gctacttctg
    45661 gaacaccgtt taagaaagag gtcgtgagcc tgtcatttaa ctctgagtca caaagagcaa
    45721 tttcaaaaaa cagcttctca tctttgatga atatcgtgac ttgtggacga ggcgttgata
    45781 agtgaatcat ctttttgtta cctatttttt ctgtaaccaa aaaacgatgg aacgttgttt
    45841 tgattgccct tggtgcggac agtctgaaca ggttccatcg tttctttatt ttctacgtct
    45901 tcttctgtgt gtgtttcgtg gttgttcttc tattgctgga tcaatcgtgc cataaaaggg
    45961 gcgattgcgg tcaaggtacc agacataaag aataaacaat gcaatacata ggacaaaaag
    46021 gattgcacct atcgtaaatc cttcaggcaa gtaactgaaa gtgacagtat gtttacctgc
    46081 tggaactttt atcatcaaga atgcgtcttt gaaattgctg accttgactg gtttcccatc
    46141 aattttagct ttccatcctt tgtcataagg aatagttgtc accaacatgg aatcttcagc
    46201 ggtatttaca gtcccagaag cttttctgcc agtggcttcc atttcgacac ctttttcttt
    46261 gatctgattc attgcggctt gataggctac aacatctaga ccaacgactt taggctccat
    46321 gaaacttatt tctttcgtcc cataaaaact tgcagtaaat gtcactgttg tctcttgagg
    46381 ataatagcca agatcatagt attgaccaga aatgttgatt tgcgtttttc gcgatgtacc
    46441 attgacagtg atcgtcgcac tagaactttc taattgcgca aaattggttg ggaacaaact
    46501 taagtaagct tgtgtatttg caggcacatg gacttcccaa gtgagctctt ttggctggtt
    46561 atctgctgtt tctgagtaag tgacgccagt cgttgtcttt gtgatcgtcg tattcaactg
    46621 attcttcaaa gtaggtgtat agaatgtaaa atactgttca tttagaccag acaatgtatt
    46681 gatcaagttt gtctgactgg tcaaattatc tgtcactggc tgctcaacat cattaacgga
    46741 caacggtgca gtaaatgcta gtggcaatgc attgacattc tgatacagtg tatagtcgcc
    46801 gctttgacca gcttggcgga agccatattt actgaacgtg ctttcgtttt tagaaatgtt
    46861 atatttgatt cctgtaaacc catccatcag caaagtatta ttgttataac ggatattcaa
    46921 atttgtccct cttgaacgga aacctaattt atccaggtaa gaggaagaat tacgatttct
    46981 tatagaagaa aacaaactga taccactgta accatagttg atcgaatcgt ttgcagatac
    47041 tggatccaga ttttccaagc ggtaaaacga gtcggaatct ttttttgttt catttacaag
    47101 agctttcaag tcaggataag gatcagtata taaactgcgg gaagcataat tccaatcatc
    47161 aagaatgcca tggatcatac tgttcgtatt gaccagtgct tcagcagaca tgatcaataa
    47221 taacaatata ctcagatgat tgaatgaaag ttttttatgc tgataaaatc ccacagcaaa
    47281 agcataaatc cctaagaaaa ccactgtcaa tacaaaggaa gtcatcgtca cataggtgta
    47341 gcttccttca ggtttgatgc cccatgccac cgcaaagaga gcagctacga ccaaaatgat
    47401 tcctaacaac agcccaccat cgtcttgttc tatcttttcc catccttttc ctgcaagcag
    47461 gagcactaag aaagagaaca gataggcata acggaataaa aacatattcg gtgcatgcat
    47521 accatgccag aacaaattca atggaacaag ataaaaactt gcgataagta tggcgaaaag
    47581 actgccgtac aaaaatttat ttttcaacgg tactttccga ctggcaaaat aaaacagaca
    47641 gaggactaat ggtagcagac ctgcgtaaac gaacggaatc gatccgtatt ttgtcgtgtc
    47701 ataaacacca atcatatttt tcatgattag atccagataa gaagtcgctt ctgttttcaa
    47761 cgtagtgatc tgactcagtg cctcgccatt tgaccgaaga tccagcactg ctggcaatac
    47821 aatgatcatt gaagcgccgc ctgccagtaa agaagtgata ccataaggaa caatcgtttt
    47881 tttatattct gaccagtttg tcaatagacg ggcgataaaa tataagaaag aaaatacgcc
    47941 aatcataaaa cccatataaa aactggataa aaacaagagt aaataactga taaataaaag
    48001 tttcggccgt ttaagatcca taacacgatg aatccccaaa atcactaatg gaagataaac
    48061 aaatgcatcc agccacatga tgatctccga atgtgcagtg ataaatgaga tcaatgcgta
    48121 actgacgcta agtgcaacat gcccccagcg aggaatcttg aacgtacgtg caagaaacca
    48181 aaaagccaat ccggcacagc cgaccttaag caaagtcaga acatacagtg catctggcat
    48241 catctggttt ggaaaaaaga acaccagtgg cgtgaagatc ccgcctagat agtaagaaat
    48301 taaagataaa taattcaatc ctagagaagc attccatgta taaaaaatac tttgctttcc
    48361 aagaagaaca tttcggaagc tggcatgaaa attagaaaac tgtgaaaagg catcacttgc
    48421 caaaatactt ctgctgcttc caggatagat tccgatactt agataaacaa gtgctaaaat
    48481 caaaaacgg
//
LOCUS       DANKAZ010000011        48062 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000011 DANKAZ010000000
VERSION     DANKAZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            59..787
                     /locus_tag="PWY67_000590"
     CDS             59..787
                     /locus_tag="PWY67_000590"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL2056898.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            787..1041
                     /gene="purS"
                     /locus_tag="PWY67_000591"
     CDS             787..1041
                     /gene="purS"
                     /locus_tag="PWY67_000591"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL2056899.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1043..1714
                     /gene="purQ"
                     /locus_tag="PWY67_000592"
     CDS             1043..1714
                     /gene="purQ"
                     /locus_tag="PWY67_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL2056900.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1715..3937
                     /gene="purL"
                     /locus_tag="PWY67_000593"
     CDS             1715..3937
                     /gene="purL"
                     /locus_tag="PWY67_000593"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL2056901.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3922..5361
                     /locus_tag="PWY67_000594"
     CDS             3922..5361
                     /locus_tag="PWY67_000594"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL2056902.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5384..6436
                     /locus_tag="PWY67_000595"
     CDS             5384..6436
                     /locus_tag="PWY67_000595"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL2056903.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6433..7011
                     /gene="purN"
                     /locus_tag="PWY67_000596"
     CDS             6433..7011
                     /gene="purN"
                     /locus_tag="PWY67_000596"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL2056904.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7008..8543
                     /gene="purH"
                     /locus_tag="PWY67_000597"
     CDS             7008..8543
                     /gene="purH"
                     /locus_tag="PWY67_000597"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL2056905.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8559..9833
                     /gene="purD"
                     /locus_tag="PWY67_000598"
     CDS             8559..9833
                     /gene="purD"
                     /locus_tag="PWY67_000598"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL2056906.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9955..10869
                     /locus_tag="PWY67_000599"
     CDS             9955..10869
                     /locus_tag="PWY67_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2056907.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11002..12567)
                     /locus_tag="PWY67_000600"
     CDS             complement(11002..12567)
                     /locus_tag="PWY67_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2056908.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12560..14011)
                     /locus_tag="PWY67_000601"
     CDS             complement(12560..14011)
                     /locus_tag="PWY67_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2056909.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14025..15041)
                     /locus_tag="PWY67_000602"
     CDS             complement(14025..15041)
                     /locus_tag="PWY67_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2056910.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15261..16349
                     /locus_tag="PWY67_000603"
     CDS             15261..16349
                     /locus_tag="PWY67_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL2056911.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16403..17929
                     /locus_tag="PWY67_000604"
     CDS             16403..17929
                     /locus_tag="PWY67_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL2056912.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17931..19136
                     /locus_tag="PWY67_000605"
     CDS             17931..19136
                     /locus_tag="PWY67_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2056913.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19393..20370
                     /gene="guaC"
                     /locus_tag="PWY67_000606"
     CDS             19393..20370
                     /gene="guaC"
                     /locus_tag="PWY67_000606"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL2056914.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20634..22058
                     /locus_tag="PWY67_000607"
     CDS             20634..22058
                     /locus_tag="PWY67_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2056915.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            22519..23628
                     /gene="pdhA"
                     /locus_tag="PWY67_000608"
     CDS             22519..23628
                     /gene="pdhA"
                     /locus_tag="PWY67_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL2056916.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23631..24608
                     /locus_tag="PWY67_000609"
     CDS             23631..24608
                     /locus_tag="PWY67_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL2056917.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            24633..26276
                     /locus_tag="PWY67_000610"
     CDS             24633..26276
                     /locus_tag="PWY67_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL2056918.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26284..27690
                     /gene="lpdA"
                     /locus_tag="PWY67_000611"
     CDS             26284..27690
                     /gene="lpdA"
                     /locus_tag="PWY67_000611"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL2056919.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            27852..28127
                     /locus_tag="PWY67_000612"
     CDS             27852..28127
                     /locus_tag="PWY67_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL2056920.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28133..28900
                     /locus_tag="PWY67_000613"
     CDS             28133..28900
                     /locus_tag="PWY67_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL2056921.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29068..30897
                     /gene="typA"
                     /locus_tag="PWY67_000614"
     CDS             29068..30897
                     /gene="typA"
                     /locus_tag="PWY67_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL2056922.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31158..32930
                     /gene="recQ"
                     /locus_tag="PWY67_000615"
     CDS             31158..32930
                     /gene="recQ"
                     /locus_tag="PWY67_000615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL2056923.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33044..33346
                     /locus_tag="PWY67_000616"
     CDS             33044..33346
                     /locus_tag="PWY67_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL2056924.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            33349..34512
                     /locus_tag="PWY67_000617"
     CDS             33349..34512
                     /locus_tag="PWY67_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2056925.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            34538..37966
                     /locus_tag="PWY67_000618"
     CDS             34538..37966
                     /locus_tag="PWY67_000618"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL2056926.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            37987..39111
                     /locus_tag="PWY67_000619"
     CDS             37987..39111
                     /locus_tag="PWY67_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056927.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39101..39538
                     /locus_tag="PWY67_000620"
     CDS             39101..39538
                     /locus_tag="PWY67_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2056928.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            39522..39860
                     /locus_tag="PWY67_000621"
     CDS             39522..39860
                     /locus_tag="PWY67_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL2056929.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            39979..40539
                     /gene="rsmD"
                     /locus_tag="PWY67_000622"
     CDS             39979..40539
                     /gene="rsmD"
                     /locus_tag="PWY67_000622"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL2056930.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            40536..41027
                     /gene="coaD"
                     /locus_tag="PWY67_000623"
     CDS             40536..41027
                     /gene="coaD"
                     /locus_tag="PWY67_000623"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL2056931.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41017..42060
                     /locus_tag="PWY67_000624"
     CDS             41017..42060
                     /locus_tag="PWY67_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL2056932.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42219..42947
                     /locus_tag="PWY67_000625"
     CDS             42219..42947
                     /locus_tag="PWY67_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL2056933.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43063..43308
                     /locus_tag="PWY67_000626"
     CDS             43063..43308
                     /locus_tag="PWY67_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2056934.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            43305..43523
                     /locus_tag="PWY67_000627"
     CDS             43305..43523
                     /locus_tag="PWY67_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL2056935.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            43659..44198
                     /locus_tag="PWY67_000628"
     CDS             43659..44198
                     /locus_tag="PWY67_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL2056936.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44191..46464
                     /locus_tag="PWY67_000629"
     CDS             44191..46464
                     /locus_tag="PWY67_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL2056937.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            46502..47548
                     /gene="holA"
                     /locus_tag="PWY67_000630"
     CDS             46502..47548
                     /gene="holA"
                     /locus_tag="PWY67_000630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL2056938.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(47637..47891)
                     /locus_tag="PWY67_000631"
     CDS             complement(47637..47891)
                     /locus_tag="PWY67_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL2056939.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
BASE COUNT        15826 a         8198 c        10657 g        13381 t
ORIGIN      
        1 aaaaacgaac aaaaaacgat gtatttgctt ttaatgtaaa taaaaaggag agacgtacat
       61 ggagaagaaa gaacttttgt atgaaggaaa agccaaaaaa atatatctta cttctgatga
      121 gaacttgatt tggatagaat atttagatca agcaactgcg ttgaatggcg cgaggaaaga
      181 ccagataagt ggaaaaggag aattgaataa tcaaatcaca agtgcaattt tccgttatct
      241 gactcaaaga ggaataaaaa atcattttat tgaacaattg tctaaaaccg aacaattagc
      301 aaaaaaagta caaattatcc ctctggaagt ggttatccga aatattgcag caggaagttt
      361 ctcaaaacgt ctaggtgttc ccgaaggtaa agaattcatc aaaccaatca ttgaattcta
      421 ctataaggat gaccagttgg atgatccatt tatcaatgaa gagcatatct atttcttaca
      481 acttgcaaaa ccggaagaaa tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat
      541 tttgaccgaa ttatttgcta aggtcggttt ggttttagta gatttcaagc tagaatttgg
      601 ccggtcgaat ggacaaattt tgcttgcgga tgaagtaaca ccggatactt gccgattatg
      661 ggatgaacgt acgaatgaac atatggacaa agacgtttac cgcagagatc taggtgattt
      721 gatcccagtc tatcaagaag tatataaccg tctatcggaa ctaactgaag aaaaggagcg
      781 agtatgatgt atttcgtaaa agtatatgta acctataaag attcagtgtt agatcctcaa
      841 ggggaggcag taaaaaaagc agtccatcgt atgggatatg aaacaatcga agatatccga
      901 ataggaaaat actttgagat acaacttgct gatgcagaag aaccaatcga tacagtgatt
      961 gaaacgattt gtgacaagct gcttgcaaat gtaaatatgg aaacataccg atatgaaatc
     1021 aaaaaaatgg aggaagtata acatgaaatt tgcggtcatc gtttttccag gttcaaattg
     1081 tgacgcagat atgttatggg cagttcgcga gatcatgggc gctgatgccg aatttgtacg
     1141 gcatgatgag tcctctttag aaggatttga tggggtttta ctgccgggag gattctccta
     1201 tggtgactat ttgagatgcg gtgcgattgc tcggttttct acaatcatgg aagaagtgat
     1261 tcgttttgct gaagaaggga aaccagtatt cggtacatgc aatggattcc agatattgac
     1321 agaagcagga cttttgccag gtgtactgag acgaaatgag tctttgcatt ttatttgcaa
     1381 aactgtacca ttgaaagtca accccaagac ggcctttact tcagagtatg aagccgacga
     1441 aataatctat cttccagttg ctcatgggga aggaaattat ttctgtgacg aagaaacact
     1501 tcaagaacta aaaaaaaata atcagattgc ctttacttat aatgacaaca tcaacggcag
     1561 cgtggaaaac attgcaggga ttacgaacaa agcagggaat gtgttaggaa tgatgcctca
     1621 tccagaacgt gcagtagaag acttgctagg atcagaagat gggaaacggt tctttgcttc
     1681 cattttaaaa aacttcggaa aggtgaccgt atagatgaaa acattgaaag aaccgacagc
     1741 tgaagagatc aagaaaaaaa gaatctatgc cgaatgggga ttgactgatg aagaatatcg
     1801 aatgatcgaa gaagatatct taggaagaat gcctaattat acggaaactg gactgttttc
     1861 tgttatgtgg agcgaacatt gttcctataa aaattctaaa ccggttctac gcaaatttcc
     1921 tactagtgga cctcatgtgt tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg
     1981 tgatggacta gcagttgtct tcaaagcaga aagtcacaat catccttcag ccgtggagcc
     2041 ttatgaagga gctgcgacag gagttggcgg aatcatcaga gatattttca gcatgggggc
     2101 tcgtccaatt gcgttgctag attcattgag gttcggcgaa ttagataatc caagaacgaa
     2161 atatttacta gaagaagtag ttgcaggtat cggcggatac ggcaattgta tcggcatacc
     2221 gactgtaggc ggcgaaattg catttgatcc ttgttatgaa ggaaacccat tagtcaatgc
     2281 aatgtgcgta ggtttgattg aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg
     2341 taattcgatc atgtatgtag gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt
     2401 tgcctcagaa gaatttaatc aagaagaaga acagcaacgt tctgctgtac aagtaggcga
     2461 tccttttatg gaaaagctgc tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga
     2521 ttctctaata ggtatacagg atatgggagc agcaggactt gtttcatcta gtgcggaaat
     2581 ggcttcaaaa gctggctcag gacttatatt gacattagat gaagtcccac aaagagaaac
     2641 tgggatgaca ccttatgaaa tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt
     2701 taaatcagga gaggaagaaa aaatccaaga actatttcag aagtatgatc tagatgcagt
     2761 cactatcggt aaagtaacag acgacgggca atatcggcta tatcatcaag gagaagaagt
     2821 agcaaatctg ccagtcgatg cattagctga agatgcacca gtttatcata aagaaatgaa
     2881 agaaccagct cgaatcgtag aatttcagca gttagctcct tatcagccag ttatcgaaga
     2941 acctggacaa gtattgcttg atctgctgca gcaaccgacg attgcttcaa aacgttcaat
     3001 ctacgaaact tatgattcac aagtacaaac caatacagtt gtccgtccag gaagcgatgc
     3061 tgctgtgatg agggttagag ggacgaataa agcgttagct atgaccacag actgcaacgc
     3121 gcgctatctg tatttggatc caaaaatcgg aggacaaata gcagtggcag aagctgctag
     3181 aaatattatt gctagcggtg gtaagccctt ggcgatcacc gactgcctga actatggttc
     3241 tcctgataaa ccagaagttt tttgggaatt atcgacttca gctgacggaa ttgctgctgc
     3301 gtgtgaaacg ttaggaactc ctgttatttc aggaaatgtc tctttatata atgaaacgga
     3361 tggtcaagca atctatccaa caccaatgat tggtatggtc ggattgattg aggatcataa
     3421 acatatcact actcaggaat tcaaaaaatc aggggatcta atctatatat taggtaagac
     3481 gtttgcagat tttgatggca gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt
     3541 gatccgaaac tttgatctat ccattgaaaa gagaaaccaa gagttagtat tgacagccat
     3601 ccaaaacgga ttgattgaaa gtgctcatga ctgttcagaa ggtggcttgg caattgcact
     3661 ggcagaatca gctttcaaac atcagctagg gatatctgta cagtttgaat tgtcctctgc
     3721 tcaattattt gcagaaactc aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac
     3781 acgattcgaa gaaatgatgg gagatgctgc tgttttagca ggtaaggtga cggatgaagc
     3841 gataatcgaa atttcagcaa ctgacggtca gatcaaaata gaaactgcag tagcaagaaa
     3901 gtgctgggag gatgcaatcg tatgtcttat gaagtgaaga gtttaaatga agaatgcggg
     3961 atcttcggtg tttggggtca tccagacgcc gctagtgtgg catacttcgg gctccatagt
     4021 cttcagcatc gaggacaaga aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag
     4081 gggcatcgag atttggggct gctttctgag gtattcaaag atcaaagaaa attgcagcag
     4141 ttaacaggag aagctgcaat cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg
     4201 gacaatattc agccttttct attcaaattt catgatcagt cgatcggctt agcgcataat
     4261 ggtaacttga tcaatgctcg ttcccttaga aaagagttag aggaaaatgg ggcgatcttc
     4321 cattctaact ccgatacgga gatattgatg catttgatcc gacgaagtga agaaacagat
     4381 tttttaaata aattaaaaag tgctttgaat gaagtcaaag gtggctttgc ctatttatta
     4441 atgacagaaa cggcaatgat tgccgctttg gacccaaatg gttttcgtcc attagcaatc
     4501 ggacaaatga aaaacggagc atacgtgatt gcctcggaaa cttgtgcatt agaagttatt
     4561 ggggcacgtt ttataagaga tgtccagcct ggtgagatag tgatcatcaa tgacgaagga
     4621 atccagattg attgctttac gaaggacacg cagctttcta tttgttcgat ggaatatatc
     4681 tattttgcca gaccagattc aaacatcgca gggatcaatg tgcatactgc acggaaaaat
     4741 atgggacgga atttagcgaa agaatcgcca gtaaaagcag atatggtcgt tggcgtacct
     4801 aattcttctt tgtcagccgc aagcggttat gcggaagcta gtggaatccc ttatgaaatc
     4861 ggattagtaa aaaatcaata cattgcccga acatttatcc agccaacgcc tgagctaaga
     4921 gagcaaggtg ttcgaatgaa attatctgct gtacgaggag tagtggaagg taaaaaggtg
     4981 attatggtcg atgattcaat tgttcggggt acgacaagca gacggatcgt ccatcttttg
     5041 aaagaagcag gggcaaaaga agtccatgtg aggattgctt ctcctccttt gaaatatcca
     5101 tgtttttacg ggattgatat tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt
     5161 gatgaaatca aagaatgtat cggtgcagat tctttaggtt ttttaagtga agagggtctg
     5221 atcgaatcga tcggtctgga aaaagatgct ccgtattcag gattgtgtat ggcttatttc
     5281 aacggagatt atccgacgcc gttatatgac tatgaagaaa aatatcagga atcactaaaa
     5341 gaaaaagtat cgttctttta aaagcagacc aagaagggag tatatgaatt caatggcaaa
     5401 tgcatacgca aaagctggcg tagatgtcga agctggatat gaagtagtag aacggatcaa
     5461 gaagcatgtc aaaaaaacgg aacgtttagg tatgatgggc gctataggag gattcggcgg
     5521 ttgttttgat ttatcggtct ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg
     5581 cgtggggacg aaattagcag tggcaatcga agcagacaaa cacgatacaa tcgggatcga
     5641 ctgcgtagcg atgtgtgtca atgatatctt agcacaaggc gcagagccgt tatatttctt
     5701 agactactta gcaacgggga aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt
     5761 agcagatgga tgtgttcaag gaaatatggc gctgattggc ggagaaacag ctgaaatgcc
     5821 tggtatgtac tcaggtaatg attatgatct agccggattt gctgtaggga tagctgaaaa
     5881 atctcaatta ttgacaggag aaacgatcaa cccaggagat gtgttgcttg gaatagcatc
     5941 tagcgggatt cattcaaatg ggtactctct tgttcgcaaa attttctttg agcagcagca
     6001 gttttcttta tcatcaaaac tgccagaatt gaaaaaaaca cttggcgaag aattgcttga
     6061 gccaacgaaa atctatgttt cttccttgct tccgctgatc aaagaaaaga aaataaaagg
     6121 agctgcacat atcactggtg gagggtttgt agaaaatatc ccgcgaatgt taccagaatc
     6181 tatggctgca caaatcaaac ttggctcatg gccgattctt ccaatattcg gctgtttaca
     6241 aaaatatggt gaaattcctg agatggagat gtatgaaatc ttcaatatgg gcatcggtat
     6301 ggtcctggca gtcgaagaat cgaaagtaaa agaaatagta cacgaaattg agcaagctgg
     6361 cgaaaaagtt tatgaaatcg ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat
     6421 aggagaagaa cgatgagaat cgctgttttt gcttctggaa acggaagcaa ctttcaagcg
     6481 ctagccgatt atctatcgaa aaaaggattg gaatcttcga ttgactggtt gttttgtgat
     6541 cagcctgaag catatgtttt gaaacgtgca actgcgttga gtgtaccggc agattgtttt
     6601 tcacctaaag aatttgattc aaaaaaagag tatgaagaag caattttgca taaattgaaa
     6661 gaaaaaaaga tcgatttgat cgtattagct ggatatatgc gaatcattgg accggtattg
     6721 ctgaaaaatt atgataaaag aatcatcaat atccatccgt ctcttttacc tgccttccct
     6781 ggtttgcatg gcatacgtga tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt
     6841 cattatatcg accaaggtgt agatactgga ccaatcatcc ggcaggaaaa agtgagaatt
     6901 gaacaggagg acacgttcga ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc
     6961 tatccagaag tgattagtga aattattgaa aatggaggct atctgaaatg acaagagcat
     7021 tgatcagtgt atcagataaa actgggattg tagcattcgc ccaaggtttg attgaacaag
     7081 gagtagaaat catttcaaca ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa
     7141 cgctttcgat cgaagaagtg acagggtttc ctgaaatgat ggatggccgt gtcaaaacac
     7201 ttcatcctaa aatccatggt ggtcttttag gaagacgcga tctccctagc catatggagg
     7261 caatggaaac ggaaaatatc caaccaatcg actttgtttg tgtcaattta tatcctttca
     7321 aagaaacgat tatgaaacca gatgtcgaaa tagcagaagc tattgaaaac attgatatcg
     7381 gtggtcctag tatgctccgt agtgcagcaa aaaaccatga atttgtcacc tctgttgttg
     7441 atccatctga ctatgaagaa gtactttcag aattacgtca gtatgggcat acttcacttt
     7501 ctttcagaca aaaactagcg gcaaaagtct ttcgtcatac tgcggcatat gatgcactga
     7561 tcgctcaata cttaacggat tgggtggaag aaaaagaacc ggaaaaattg acacttacgt
     7621 atgaacgaaa acaaacgctt cgttacggtg aaaatagcca tcaagccgct gccttttatc
     7681 agtccgctct tctggaaaaa tattcgatcg cagcagcaaa acaactccat gggaaagaac
     7741 tttcttataa taatatccga gatgcagatg ctgcgctacg gatcatgaag gaatatgacg
     7801 agccaacggt agtcgccttg aaacacatga atccttgtgg tattgggaca gcatctacga
     7861 ttttagaagc gtgggaagca gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca
     7921 tcgttttgaa tcgagaagta gatctgccaa cagcagaaca gatgcataag ctgtttctag
     7981 aaatcatcat tgccccttct tatacacaag aagctttgga attactgcaa aaaaagaaaa
     8041 acatccgatt actgacagta gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg
     8101 tctcagtctt aggtggactt cttgtacaag atcaagatct ggcaatagaa aatgaagaag
     8161 agtggaaagt cgtgacaaaa cgtaagccga ctccagcaga agcaaaagca ttagcgtttg
     8221 cttggagagc tgtcaaacat gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga
     8281 cagtaggaat cggagccggg cagatgaatc gagtcggttc agtaaaaatt gcgattgaac
     8341 aagcgcaaga gaaaatggaa ggtgcagttt tagctagcga tgcttatttt ccaatggatg
     8401 attcggttga atatgcagcc aaacatggta taaaagcaat cattcagcca ggaggaagca
     8461 tcaaagacca agattctatt gatatggcga ataaatacga tctagcaatg gtctttactg
     8521 atgttcggca ttttagacat tagagaggag tcggataaat gaacattttg gtgatcggtg
     8581 caggtggtcg tgaacatgca ataagtaaaa aacttttagc cagtccgaag gtggatacgg
     8641 tgtattgtgc gccaggaaat ccggggatga caaaagatgg tatcaattgt gtcgctatca
     8701 gtgagcgaga tcattcagcg ttaatccatt ttgttaagaa agaggctatt gattggagta
     8761 tcgttggtcc tgaagttcct ttgttaaatg ggatcgtcga tgattttatg gcggaaggat
     8821 tgaaaatctt cggtccgaca aaagcagcag ctctgatcga aggttcgaaa agttttgcta
     8881 aacaattaat ggatacttat caaatcccga cagctaaatc cagaagtttt gaatgttacg
     8941 aaaaagcagc agcgtatatc aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct
     9001 tagcagctgg gaaaggtgta gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag
     9061 aacaaatgtt gaaccagcat cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt
     9121 ttttggcagg agaagaattc tcccttttgg cgtttgtccg taataccaag gtgtatccga
     9181 tggtcatttc gcaagatcat aagcgggcat ttgataacga taaagggcca aatacaggcg
     9241 gaatgggtgc ttattcacca gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg
     9301 aagaagtttt aaaaccagcg gctttgggaa tgaaagaact aggacgctct ttcacgggtg
     9361 ttttatatgc gggtttgatt gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc
     9421 gtttcggtga tcctgaaacg caagtcgttc tttctcggtt gaagagtgac tttgcgcaag
     9481 taatcgatga tttattagag aatcgtacag tagagttaaa gtggcagcaa gaaggataca
     9541 atgtaggcgt tgtggttgca gctgccggtt accccgaaga atatgagaca ggaatggttc
     9601 taccagattt tctggaagaa gagctttctg tttactatgc tggagtttcc tcaaataacg
     9661 gcaaactagt tggagcagga ggacgtcttt atttagtaga agcatacggt gagacgatca
     9721 aagaagcaca gaaaaaaatt tacaccgcgt taatggataa aacgacagat ggaacatttt
     9781 atcgtacaga tattggagaa aaagctgcca atagcgaact gatcgtgaag taaatcagct
     9841 gcttccattt ataaaaaata caacaaacat aatcggaagc gccgatttta gtgaagcctt
     9901 ttcataaaga gtgtggtaca cttttgttgt gtatataaat aaggaggagt tcagatgaaa
     9961 aaaattcaat taggtacttc tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt
    10021 atcaacaatg cggaagatcc agtaaaagtg attgagacag cttatgaaaa cggcattgat
    10081 ttttttgacc atgcagatat ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct
    10141 ctagcaaaaa catcaatcaa acgagaagat ctttttattc aaacaaaatg tggaatcgtt
    10201 ccaggtgtga tgtttgattt ttctaaaaaa catatcattg aatctgtaga aaaaagtttg
    10261 acccgtctga aaatggatta tgtggatgct ttacttcttc atcgtccaga tacattggta
    10321 gaaccagagg aagtagctga agcgtttgat gaattggaac ggtcaggaaa agttcgttat
    10381 tttggcgtca gcaatcaaaa accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa
    10441 ccattagtag ctaatcagct gcaatttggc ttgaaacata ctggaatgat cgatcaaggg
    10501 attcatgtca atatgacaga tgaagcaagt atcgatcatg acggtagtgt acttgattat
    10561 tcccgattgc atgatatgac gatccaagca tggtcaccat accagtatgg attttttgaa
    10621 ggtgtattta tcgggaacga aaaattccct gaattaaacc aaacattaga agaactagca
    10681 gctaaatatc aaacgacacc aactggcttg gcttctgctt ggattttacg ccatccagcc
    10741 aatatgcaag tgatcgccgg tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct
    10801 tcagaaatta atatgtctcg ggaagattgg tacgaattgt accgtgctgc tggaaacatt
    10861 ttaccttaaa gattgtcata agcaggtcaa aagggctata aattaaaaag acagaataac
    10921 ttgaaagctg ttttcagctt cgagatattc tgtctttttt tattgcaaat ggaaagagta
    10981 tcattgtacg gatttcgttt attagttatt tttgttttta aggaaaagct gtgtccgata
    11041 ttcttttgga gaaatccctt gttttttctt aaataaataa ctgaaatagt tagggtcatt
    11101 ataaccaatt tcaaaagcaa tatctgaaat acgatagtct gattgctgca ataatttttt
    11161 tgctaattcg attctttgtt ctgtcagata atcgatgaat gtttggttaa acgattgaga
    11221 aaaaatcgta ctgaaatgag agggacttaa tgcaacttgt tgagcgaccc aagttaagga
    11281 catatctgga tcggtaaaat tttctttgat aaaatccaat gcatggttca aaattggctg
    11341 atatttgttc cttgaaggct gtgagggcag agcagttaag aaagaaatga acatataaac
    11401 tgtcgaacgg taaagcgagt cgtcggcgct gattgctaag cgttgactgg tggatgtgca
    11461 atgaaccatt tcttgcggga ttttcttttc tttcaggcag acatgctgga gtagcttagt
    11521 caagacaaat aaacgatata atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg
    11581 ttcgatgtac ttagatactt cgtttttcga taatgaagac aaatcaagtg agaggtcaaa
    11641 gtaaaagaca gaatcaactg gaagtttcgc ttcttgaatc aaatcctcat agctgataat
    11701 ttgttcttta agggaactgg cggtttcaag tatcatctgc gtgatctcaa aagatgcagg
    11761 taattcgctg attctttctg cgacaggacc aatggcgaca gaaaaattat ttttttgtcc
    11821 cttttctaat tcatggacca acgtttgggc tacctgataa ctggtctcga tcacattttc
    11881 ttcaagagaa tcaactatta aaaatttgat gaatcgagag gaaacagcag agaagatcac
    11941 ttgttgattc tccctaaata aaaacttcaa ataatcactg agatgagcgt aatcttcaaa
    12001 gtctgtactg acttggtttg ctgctaataa tacggtcatt tttttccctg ctaaagagcg
    12061 attgaaagat tcagcaagtt tcaatgtttc actgatcgta agtgagcctt tgaacaaacc
    12121 gttcaagaaa tgattttttt tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt
    12181 ttgctgcaaa tctaaagcag ttactgcttt ttctattgct tgtttcaatt cgctactttt
    12241 aatcggttta agcagataat catctgcctg tacctggatt gctcctttca tataatcgaa
    12301 atcgtcgaat ccactaataa atatgagacg agtccaagga agtagtttcc ttgcttcttt
    12361 tgaaaaagtc aacccgtcca tgaaaggcat ttggatatca gtcagaatga tgtcgggctg
    12421 taagtcgata attgaagaca gtgccatctc accatctcca gcttcgccga cataagtgat
    12481 tggataataa gaaccaagga cagttatttg ttcccgcaag ttattgcgaa tgaggtattc
    12541 gtcttcgatg ataaaaactt tatacatgtt tgtttcctcc aattgttttg ggagatagag
    12601 ggatcatcag tgtgacagtt gttccttctc ggtatttgct gtcaatcgtg ataggttttt
    12661 gatcatttcc gtaataaaga aggagacgct tgtttacgtt gtaaagtccg tagccgatac
    12721 tgaaatctga atcgatccct ttggctaact ctttacgtat ttctgccagt ttttctgcag
    12781 ggatgccaat cccgttgtct ataactttga attggatcat ctcatcaatt ttgcatgcgg
    12841 taatacgaat aaatcctttg tgacggacgt acttcgttcc atggtataca gcattttcaa
    12901 tcaatggctg caagatcatt tttaaaatag aaacaggaaa aagtgtttct ggtatatcga
    12961 tcttatagtc aagcatatgg ccgtatctta tttttaagat gactagataa tcattgatat
    13021 ggctaatttc tttttcaaga ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa
    13081 agccggaaag tgcatgggtc atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag
    13141 aaataatggc atccaaactg ttataaataa aatggggagt aatctgagct tgcaatacac
    13201 gaacttcact ttgtgctaaa tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta
    13261 gatcttccgc cattttattc atgctagcgg taagtgtttg taattccagt gtttccggca
    13321 gggttaaacg aaagccaagg ttccctgaag aaagttgttc tgacatctcg atcagctgat
    13381 tgatcggttc ttggaccttt ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa
    13441 tggtactaag aactccaagt tccactattg ttaatagaat gagcgagcgg acaagtgtcg
    13501 tatttttttt gcttgctaag tcgatctcca cacgaacaaa atcttgtaag atatctgaca
    13561 gcagttgagt gacagaatct acctgattga tgatctcttc attggtatcc ataaaatctt
    13621 ttgccaagat attattgatc agctcgtttt gataatcttc cagtgtatcg atcgtacgta
    13681 gcgatacatc taacgtacta atttctttaa gtgtatttgt attatttttg atctctcgaa
    13741 tatcattcct aagttcttct aggatatttt cctcattgaa ctcatctacg gttacttggc
    13801 cagaaatcaa gtcccacata ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat
    13861 ctgctttttg gatatttgcg atcgtctgtt gataaatcaa aagctgttgc gaatataaca
    13921 gtatgctcag tagcaaaggg ataacagcaa atagaagcat ggctttgttt gcttgggcaa
    13981 aaatctgttt tatgcttaaa ttttttgtca tgatacttct ccagtcaata atttctggtt
    14041 gggatgttag ctatattctg ctgggaaaag acagtctcgg aaataaaaat atctttaggt
    14101 aatgtttttc ctgtttgaat atttccaaaa taacgagtaa cggtattggc tacataccag
    14161 ccgacatcag gtttacactc gactacacaa ttgacgattc ctgagcgtag cttctcgatc
    14221 atatcttctt gtccatcaat agttacaatg atcaaatctt ctccaggttt gattgatgtt
    14281 tcttttatcg cagctaaagc accgtgagtc atttcatcac tatggctgta taatacatcg
    14341 atttcggata aatcatttgt ttctaaatat cgtttcatct ggtcttttcc tttcatccga
    14401 ataaaatcac cgacaattcg gtgaacaata gtcatcggag gtttatggtc gatggttcca
    14461 tcttttttta gggttggttt acgattgatt gattccaaaa atccttcgga gcgtagcttg
    14521 gtcggtgtgg agttctctga accagcaagt tctaggattc tgatttcctg tttctttgaa
    14581 tttgtgaagt gattgctgac aaataaaccc gcccgatttc cttctgcttt gaaactgggg
    14641 ccaatgtggg tgacgaaaag ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg
    14701 acagggatcc ctgctcgctt tgcttctttt aatacagagt cccacccgga ttcttgcaaa
    14761 ggagtaaaaa tgatcatatc cactttatag gcaatgaacg tccgtatatc ttggatttgc
    14821 cgttcctgat tcatgtagcc atttcggtag agaacttggt aatcttcttt ttcaagcgca
    14881 gtgataattg attcagtatg ctttttccgc cagctactct ccgttcctga ttgcgaaaaa
    14941 ccaacgacta gtgggtcagg tgcttccttt gtattaggtg tacaccccat catttggtaa
    15001 gcgattacaa caagggctaa aaaaaataaa acacgtttca ctttaaatca ctccatctgg
    15061 atttgttagt actatcttat cagataaaag ggaaaatcaa agtcaaaagg aatcaatcga
    15121 aaaaaatcca agaaacttaa aaaaatccaa gacaaaaaaa gtacacctaa aaaatttaaa
    15181 gtaaatccca taaaaaaatc aatgttaacg ctttctttat tagctatact cggtgtatca
    15241 attgaaatgg gggataaaaa atgaaaaaca aatttagaaa gattgttggg actatcgcta
    15301 ttttgaccgc aagcattcta ttaagtgcgt gtggcaattc aggatctgca gatgatagta
    15361 cggggtatgt aggaatcgct atgccgacca aatcagcaga aagatggatc gctgatggga
    15421 acaacatggt ctcggaatta gaaaaactgg gttataaaac agatcttcaa tatggagaag
    15481 ataaagtaga aaatcaagta gcacaaatag aaaatatgat tacaaaaggg gtagatacac
    15541 ttgtgattgc ttctatcgat ggttcggctt taactgatgt gttagctaaa gcaaaagaag
    15601 cagatatcaa ggtgatcgcc tatgatcgtt tactgatgaa ttcagaaaat gtcgattatt
    15661 acgcgacttt tgataatttt ggagtaggtg tatcgcaagc agcttatatt gaagagcact
    15721 taggattgaa agaaggaaaa ggaccattca cgattgaatt atttggtggc tcgcctgacg
    15781 acaacaacgc actaatcaat tacaacggag tgatgtctgt tttacaacca tatatggaca
    15841 acggtcagtt ggtcgtgcca tctggacaga cgagtttcag tcaaatcgct acgcttcgct
    15901 gggatggttc tacggcgcaa gcaaggatgg ataatctgtt aagcgcaaat tatactgacc
    15961 aaacattgga tgctgttttg tcgccatatg atccaataag tttaggaatc atttcgtcat
    16021 taaaaggcgt ggggtatggg agtgaaagta aacctttacc tgtgattact ggacaagatg
    16081 cgacagttgc tggcgttaaa tcgatcattg ccggggaaca aacacaaacg atctttaaag
    16141 acacacgtat cttagcaaaa aatacgattg aaatgatcaa agcaatcagt gatggagaag
    16201 aggttccggt caatgatact gaaacttatg ataatggggt gaaaactgtc cctacgtatt
    16261 tagcaaatac agtatctgtc gataaagata attatcaagc agagctgatc gacaccgatt
    16321 attataaaga atccgatctg aaaaattaag aggaaccaaa caaggaacta aagagatcta
    16381 agtcagagag aggataagaa ttatggcgga ttgtatttta aaaatggcag atatcaccaa
    16441 aaaattttct ggaatccgag cgttagattg cgtgaatctg atgattgaac gtggggaaat
    16501 ccatgcattg tgcggagaaa acggagcggg aaaatcaaca ttgatgaatg tcttgagcgg
    16561 cttgtatcca tatggctcct atgacgggga aatcttttat gatggaaagc tgtgtcaatt
    16621 taaagatttg aaagatagtg aagaaaaagg catcgtcatc attcatcaag aattagcact
    16681 aagtccttat ctttcaataa aagagaatat ttttctcgga aatgaacaag cagaaagagg
    16741 cgtgattgat tgggaactga cagaaaagaa aacatatgaa ctgctgaata aagtcggttt
    16801 gtctatcgac ccgaatcttc tagtatccca gattggcgta gggcaacagc aactagttga
    16861 aatagctaaa gcattatcaa aatccgtccg tctgttgatt ttggatgagc ctaccgctgc
    16921 attgaatgaa gaagaaagcg cgaatttgct gagattgatc cgagagttga aagagcaagg
    16981 tgtaacctcc atcattattt cacataagct gaatgaaatc gtggatatcg cagatcgaat
    17041 caccatttta cgggatggac agacgattga gacactagaa aatgaaatga tcagcgaaga
    17101 gcatattatt cgcgggatgg tgggcagaga tttagttgat cgttatcctg aaagaaaacc
    17161 ggatattgga gaagtttatt ttaaagtaga agactggacg gtctatcacc ctcttgatca
    17221 cgagcgaatc ataaacgacc acatcaactt gcatttgaaa aaaggagaga ttgtaggaat
    17281 agctggactg atgggagcgg gcagaactga attcgctatg agtctatttg gacattctta
    17341 tggaagcaag atatccggac gtatattaaa ggaaggggca gagatatcag ttaagaccgt
    17401 tccttcagcc attgcaaacg gactcgctta tgtctcggag gaccgaaaat ctttaggact
    17461 gaatctgctg atggatattc gggaaaacgt gacgctgagc agtttggata aaataagtca
    17521 aaaaggagta ttagataaag aaaaagaagt aactgaggca gaaaatttcc ggaaaaagat
    17581 gcgtataaaa acaaactcca tccatcaaat tgtcagtagt cttagtggtg ggaatcaaca
    17641 aaaagtcgtt ttatccaagt ggctgatgac acaacccgat attctttttt tggatgagcc
    17701 aacccgtggg atagatgttg gggcaaaata tgagatctac acgattatcg aagaaatggc
    17761 agcaaatggc aaagctgtct gtcttatctc ttctgaactt cctgaaatat tagggatgtg
    17821 tgatcgaatc tatacgatga acgaaggacg tattacagga gaagtctcgc gagaagatgc
    17881 agatcaggaa gtcttgatga aattaatgac gaaagaagag gtgggttaag ttggaacagg
    17941 caaaaaatct tacggataaa aaagagaaaa aactaaattt gaaaagtaga gcagctgatc
    18001 tttttggtcg ctacagcatg gtgatcattt tagcgggact acttattgcg tttcaattga
    18061 tgacggatgg tattttctgg cgtccattga atataactaa ccttgttctg caaaatagcc
    18121 atatattagt actagctgca gggatgttat tagttgtgct cttaggtcat gtggatttgt
    18181 cggtagggtc agtgatggcg tttgttggtg caattgcggg catgatgatg gtcaatcgcc
    18241 aaatgaaccc gtggatgaca gttatcgcat gtcttgcagt aggcgggctg atcggcgcat
    18301 ggcaaggttt ttgggtagct tatgcaggga taccggcgtt tatcgtgact ttagcaggat
    18361 tgctgatgtt tcgtggactg acacaagttg ttctaggagg ccaatctcta gcaccttttc
    18421 cagctgtttt ccaaaaaatg tctacgggtt atttaccaga cttaagcgaa ggaagcagcc
    18481 ttcacttctt gacattgatt ttaggaatga tcatcacgat cgtgttagtt gttggtcaat
    18541 ggcgaacaag aaatcgtcgg aaaaatcatt tatttgaagt ggaatctcca acagctttct
    18601 tggtaaaggc ggtatttaca gcagtcgttg tactaggaat aacttatatc tttgcctctt
    18661 atcaagggta tccgattatt ttgatcattc ttggtttgat cgttgcagca tatgggtttt
    18721 tgacaaataa aacagtagca ggtcgacaga tttatgcgac gggcggaaat cgaaaagcgg
    18781 ctgaactatc agggatcaaa acaaagcgaa tcacgttctg ggtattcgtc aacatgggag
    18841 tcatggcagc tttagctgga ttagttttag ctgctcgatt gaatgcggca acacctcaag
    18901 caggtacatc gatggaactg gatgcaatgg cggcagtata ttttggtggt gcttcgactt
    18961 ccggcggcat tggaacaatc atgggtgcga ttgtcggtgg cttggtcatg ggtgtattga
    19021 acaatggcat gtcgatttta ggcgtcgggg tagactggca gcaggcaatc aaaggattga
    19081 tcttgctttt agccgttgtg ctagacttgt ataataagaa aaaacgaaca acgtaggaga
    19141 acgccggtta ttcagaaaaa agactgtgac aagacatttg tcacagtctt tttttcgcta
    19201 aaagcgaaca ttcattgttt cgtttgtata gactaaaaat aatatatgtt tttttaccat
    19261 tcgtataaat tctctttttg ctcttgattt ttttgcttaa ttgagttaaa ttacataggg
    19321 cgctaaaaaa agccttgatt aaattaataa tgttcgtgtt ttgctggatg tgaacatcag
    19381 gaggataatg taatgaaagt atttgattac gaagatattc aattaattcc taataaatgt
    19441 atcgtaaata gccgttctga atgtgataca acggtcacat taggtaaaca tacattcaaa
    19501 atgccagtag tgcctgccaa catgcagacg attattgatg aaacaatcgc agaattctta
    19561 gcggaaaatg gttatttcta tatcatgcat cggtttgatg aagcagctcg aattccgttt
    19621 atcaaaaaaa tgaaaaagcg tggattgatt acctcgatca gtgttggcgt caagaaagag
    19681 gaatattcct ttatagagaa gttggcagaa gaatcgctaa atcctgatta tatcactatc
    19741 gacatcgcgc atggacatgc taactcagtt attgatatga ttcagcatat taaaaaatat
    19801 ttgcctgaaa cttttgtgat tgccggtaat gttggtacac cggaagccgt ccgtgaattg
    19861 gaaaatgcgg gcgcagatgc aacaaaagtc gggattggtc caggaaaagt atgtattaca
    19921 aaaatcaaga caggctttgg gactggaggc tggcaactgg cagctcttag atggtgtgcg
    19981 aaagcagctc gcaagccgat catcgctgac ggtgggatca gaacacatgg cgatattgca
    20041 aaatctgtcc gttttggtgc gacaatggtc atgattggtt cattgtttgc cggtcacgag
    20101 gaatctcctg gcgaaacaaa agtcgaaaat ggaatcgtgt ataaagaata ctttggttct
    20161 gcttcagaat tccaaaaagg cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa
    20221 cacaaaggat ctttgaaaga tacactagtg gaaatgcaac aagaccttca atcatctatt
    20281 tcttatgctg gaggaagaga tctggaagcc attcgaaaag ttgactatgt catcgtgaaa
    20341 aattcgattt ttaatggaga cacaatctaa aaaataaata aaatattagg atgttgtgaa
    20401 aaagttgtcc tgcattaagt aataagaaca gccaaacaaa aatagttctt tatatgtttt
    20461 gtttggctgg aattattatc gcagatacaa gcttttgaac atcattattc agaataaggg
    20521 actgtgataa gatttttgtc acagtccctt atgttttgca aaaaaagttt ttcttttttc
    20581 tcgtaaattc ggtaaaatca aaagaaaaaa tagacaatag aaaagagaag cttatgtggg
    20641 aaaaagtaga gaaaaaacaa ggaccgattt atcgtcaaat tatggaacag attatgagag
    20701 aaatcgaaaa cggaaggatc aatcctggaa gtcaacttga atcagaacga aaactagggc
    20761 aactttttgg tgtgaatcgt tccaccgttg tccatgcatt ggaagagctt agggagcttg
    20821 gggtactaac gagcaaacgt ggcagcggat ggtttgttaa tcagactgaa tgggggaaat
    20881 tcgctgttcc aagaatcgat tggcggcaaa tgatctctcc tcgttatgag cagacggact
    20941 ggtatgagca aaaaataaga gaagcaaagc agatccaaaa aacatcgttt cttgatttat
    21001 acgctagcga gatgccagat gagcttttgc cgaattttga gtttccttct tattctcttg
    21061 aagaaatctt agcggaagaa aaagaaatgg atcttttagg ctatactccg ttacgtaaca
    21121 aaatcaaacg gtatttggaa agtaaatttg aattggcatt ttcccccaac cagcttcttg
    21181 tgactggtgg cggtcagcaa gcaatctttt tgattttaca aacgattctt tcagctggag
    21241 aggggatagc tgtagagtca ccttcttttt tttaccgatt acctcttttc aaagcttcag
    21301 gaacgcgttt atttgggata ccgatggatg aagaagggat cgacttagaa aaactgaaag
    21361 aatcgatcca taaaaataaa ttgaaagcag tgcttatcaa cccgaatttt caaaacccta
    21421 caggaaaagt gatgagtcaa aaacgacgag aaaagctagt agaattgtgt cgacagtatc
    21481 ggcttccaat tattgaagac gatgtattta cggatctttc atttctaaac caagaagaag
    21541 tccttcctat acgaacgatt gatccggata atgtgttata cgtcggttcg ttatctaggg
    21601 ttttaggaaa aacaacgaaa atcggttggg tgataggacc tgagactttt atctcgcaac
    21661 tagctcaagc gcaagaaatg atggagtttt cgatgaatat ccttacacaa atcacagtgg
    21721 ctaatgtatt taatgaagca attgatttga aaatgcaaga tgtaagaaaa aagttgaaag
    21781 aaaaaagccg actgataagc gcttggggag attcacaatc gtttttcaag cttcattcaa
    21841 ttttaggtgg ttactatgcc tggattactt gggatgggaa aaaactagac cccgaactag
    21901 cagaaaaagt agtagatatg ggtcttggaa ttgcaccaag tctacttttt gggcaagata
    21961 taaatggaat cagaatcaat tacagtaggc tggatgaaaa ccaaataccg ttgttttatt
    22021 caaagatgaa aattctagaa aagtggttaa cagaataatt tcacaaagta aatagattaa
    22081 agaatataac agcttggatt taaatttcaa gctgtttctt ttttttcgga aaaagaatac
    22141 gatataaaga aactaagaaa aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa
    22201 ataaatgttt gcaacaaaac tgtataacac caacgttttt aaatagaaac agatggttgt
    22261 aagtttgatt agaaaataga aaaaatgcag atgaaaaata aaacgctttc cttattgtga
    22321 ttttattcac taatgctgtt aatatgcgga ttggcataat ttcctttcat cgtgaaaatt
    22381 tgtgctttta gcttgtattt ctcataaatc catgataatt tattaatgaa gacggttaaa
    22441 tagtgataca gttttattca ctgtttcaat cgcagaatga agagattttc ttatagatga
    22501 gagaggaatg tgtgacaaat ggcaaacaaa aaagcaccga tcgacttcca agcactgcta
    22561 gatgaagtca atgcagattt cccagtatat caagcacttg atcaagatgg aaacgtagtt
    22621 aacaaagatt tagtacctga tttatccgat gatgaactcg ttgaattaat gactcatatg
    22681 gtatggtctc gtgtattaga ccaacgttca acagcgctga accgccaagg acgcctaggc
    22741 ttttttgcac caactgctgg acaagaggca agtcagctgg ctagtgctta cgcatttgat
    22801 aaagaagatg ttcttcttcc aggttatcgg gatgtacctc aactagtaaa acatggttta
    22861 cctttatctc aagctttttt atggtctcga ggacatgctg caggtaattc ttatccggaa
    22921 gaattgaaag ctctaccacc acaaattatc atcggtgcac aatatgtaca agcagccggt
    22981 gttgcattag gtttgaagaa acgaaacaag aaaaatgtcg tcttcactta tactggtgat
    23041 ggcggttcat cacaaggtga tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca
    23101 aatgccgtat tttatatcca aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa
    23161 actgcagcaa aaacacttgc acaaaaagct gtcgcagctg ggattcctgg tattcaagtc
    23221 gatggaatgg atccgcttgc agtatatact gtatctaaaa tggcacggga atgggcgatc
    23281 agtggaaatg gaccagtcct aatcgaaaca ctgacttatc gttatggacc acatacacta
    23341 tctggtgacg atccaacccg ctaccgtaca aaagacacag atgacgattg gcagaaaaaa
    23401 gatccattga tccgcttccg taaatatttg acggaaaaag gactttggtc tgaagaaaaa
    23461 gaagaacaag tcatcgaagc aactaaagaa gaaatcaaag cagctattgc tgaagccgac
    23521 aaagtaccaa aacaaaaggt ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa
    23581 tcaatcaaag aacaaattgc aatttatgaa gcgaaggagt cgaaataatc atggcacaaa
    23641 aaacaatgat ccaagcaatc acagatgcct tagcacttga actagaaaat gacgagaatg
    23701 tcgttgtttt tggtgaagat gttgggaaaa acggaggagt tttccgggca actgaaggat
    23761 tgcaggaaaa gttcggagaa gaccgagtat tcgatactcc tttagctgaa tctggaatcg
    23821 caggattgag tttcggtctt gcgttagaag gatttcgtcc agttcctgaa atccaattct
    23881 ttggttttat tttcgaagca atggacgaag tagtagctca aatggctcgt acaagatatc
    23941 gaatgagcgg cactagaaat ctacccatca caattcgttc gccatttggt ggcggtgttc
    24001 atacaccaga attgcattcg gataatttag aaggtttgat cgctcagtct cctggtattc
    24061 gagtagtgat tccttccaac ccttatgatg caaaaggact attgatttct tctattagaa
    24121 gtaacgatcc ggttgttttc ctagaacaca tgaaactata tcgctcattc cgagaagaag
    24181 tgccggatga agcttacgaa gttcctttgg ataaagcagc agttactcgt gagggaacag
    24241 atgtatctat tatcacatac ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc
    24301 ttgcaaaaga aaatatatct gttgaaatca ttgatcttcg tacagttgcg ccattagatg
    24361 tagaaacgat cattcaatcc gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac
    24421 aacgacaagc gggcgttgcc gcgcaagtag tttctgagat ctcagaacgc gctatccttt
    24481 cattagaagc cccaatcgga cgagtttctg cacctgatac agtcttccca tttggacaag
    24541 cagaaaatgt ctggttgcca aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg
    24601 aattttaaga aaagaaggga cgaaacaaag tcatggctta tcaatttaaa ttgccggata
    24661 tcggtgaagg aatcgcagaa ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga
    24721 tcaatgaaga tgatacatta ctagaagttc aaaatgacaa atcagtagaa gaaatcccgt
    24781 cacctgttac aggaacggtt aaaaatgtga ttgttccaga aggtaccgta gcaaatgttg
    24841 gggatgtatt agtagaaatc gatgcaccag gacacgaaga caatgaaggg gacagtggtg
    24901 tagccgcaga atcccaaact ccagctaaac cggctgctga acccactgtt gatacagaat
    24961 cagctggatc gtcaagcgaa ggagtcttcc aatttaagtt accggatatc ggtgaaggaa
    25021 tcgcagaagg tgaaatcgtc aaatggttcg tcaaaccagg agatacgatc aatgaagatg
    25081 atacattact agaagttcaa aatgacaaat cagtagaaga aattccgtca cctgtcacag
    25141 ggacagttaa aaacgtgatt gttccagaag gcactgtagc aaatgttggg gatgtattag
    25201 tagaaatcga tgctcctgga cacaatagtg caccgtcaac tagtgcgcca tcagctgaag
    25261 caccaaaaga aaaagtggaa acatcaggtt cagcaagtgt cgttgaagct gccgatccaa
    25321 ataaacgtgt attggctatg ccatctgttc gtcaatttgc ccgtgaaaaa gatgtagata
    25381 tctcacaagt aactgcaact ggaaaaggtg gcagagtaac aaaagaggac atcgaaaact
    25441 tcttggcagg tggtccaagc agtgcaccag cgaaatcaga agcgccagaa gcggcagcac
    25501 cgaaagaagc agctcctgca gcagaaagca aaccagctgc accagcaaaa ccattcaagt
    25561 ccaacttagg agacttggaa gaacgtgtag caatgacgcc aacacgtaaa gctatcgcta
    25621 aagcaatggt caacagtaag catacagctc cacacgtgac attgcatgat gaagtagaag
    25681 tgtctaaact atgggataat cggaaacgat ttaaagaagt tgctgctgca aatggtacga
    25741 aacttacttt cttgccatat gtcgtgaaag ctttaacagc gactgttaag aaatacccag
    25801 tattgaatgc atctatcgac gatgcaaacc aagaaatcgt ttataaacat tactacaata
    25861 tcggtattgc aactgatacg gatcacggac tatatgtacc aaatgtgaaa gatgcagacc
    25921 gcaaaggaat gtttgctatt gcagacgaaa tcaatgagaa agcaaaatta gctcatgacg
    25981 gcaaactatc tgcagaagat atgcgcaatg gaacaattac gatcagcaat atcggatctg
    26041 taggtggcgg ttggtttaca ccagtcatca actatccaga agtagctatt ttaggtgtag
    26101 gaacgattgc acaacaacca atcgtaaatg ctgaaggcga aatcgttgtc ggtcgtgtga
    26161 tgaaactgtc attaagtttc gatcaccgta tcgtagatgg agcaactgcg caacaagcaa
    26221 tgaataatat caaacgtctt ttagctgatc cagagctatt aatgatggaa ggatgataaa
    26281 caaatggtag ttggagattt tgctgtagaa ttagatacag ttgtaattgg tgcgggacct
    26341 ggtggctatg tagctgccat ccgcgctgct gaaatgggac aaaaagtagc aatcattgaa
    26401 agagagtata ttggcggtgt ttgtttgaat gtcggctgta ttccttcaaa agcactgata
    26461 gctgcaggac accattatca agagtcacta gattctgaaa tgtttggagt aactagtgaa
    26521 aacgtcaaac tagattttgc aaaaacacaa gaatggaaag aaaacaaagt tgtccatacg
    26581 ttgacttctg gtgttggttt cctattaaaa aaacataagg ttgaaacaat cgaaggagaa
    26641 gcgttcttcg tagatgatca cacactacgc gtcattcatc cagattcagc tcaaacttat
    26701 tcgttcaata acgcaatcat cgcaacaggt tcccgtccaa tcgagattcc aggatttaaa
    26761 ttcggaggac gtgtgttaga ttctactggc ggattagcat tgaaagaagt acctaagaaa
    26821 tttgtgatca tcggtggcgg tgtgattggt gccgaattgg gcggagcgta cgcaaactta
    26881 ggagcagaag taacgattct tgaaggctca ccacaaattt taccaactta cgaaaaagat
    26941 ctagtaaaat tagtagaaga tgatttcaag aaaaaaggcg taacagtcgt aacgaatgca
    27001 atggcgaaag aagcagtcga taacggtgac agcgtgacag tgaaatatgc agtggacggg
    27061 aaagaagaat ccgttactgc tgattatgtg atggtcactg taggtcgtcg tccgaataca
    27121 gatgatatgg gcttagaaca agcgggtgtc gaagtaggag aacgaggatt gatcacggtg
    27181 gataaacaag gacgtacgaa cgtacctaac atctatgcca tcggtgatat cgttccaggg
    27241 gcggcattag cacacaaagc cagttatgaa gcaaaaatcg ctgctgaagc aatctctggc
    27301 aaaaaagtag ctgtagatta taaagcaatg ccggcagtag ccttcactga tcctgaattg
    27361 gcttctgtag gtatgacgat caaagaagca aaagatgctg gtatcgaagc aaaagcatat
    27421 aaattcccat tttcagggaa cggtcgggca ttatcgcttg gtaaaacaga aggattcatc
    27481 cgtctagtta cgacgatcga agataacgtg cttattggcg cgcaaatcgg tggagtcggc
    27541 gcaagtgata tggtctctga attagcttta gcaatcgaat caggaatgaa tgctgaagat
    27601 atcgccttga cgattcatcc tcatccatct cttggcgaaa tcgtcatgga tgcttcggaa
    27661 ttagcattag gtttaccaat ccatatttga tttgaataat acaggcgaaa cgcaactgaa
    27721 aaatcagttg cgttttgttt tttagaatta tcaataatat tattatgtaa acttaaaaat
    27781 atgacaactt cttggtttat taccacttcc tatgctacac tgaagaggaa tatgaaatag
    27841 gagagttgaa gatgcaagaa tatcagtatc cacttgattt agattggaca acagacgaaa
    27901 tggtcattgt aatgaagatg tgggaagatc tggaacgtgc gaatgaaaaa gggataaaca
    27961 atgaagcttt tttgaagact tatcagcagt ttaaaactgt tgtcaaatcc attggtgagg
    28021 aaaaacgatt gggacgggaa tttgaaaaag cttccggtta ttctttatat cgtacagtgc
    28081 agcaagcaaa aaaaaatcca aacaaaactt tgagaatgaa cgggtgagat aaatggaaca
    28141 gatgattgat catatcaagt cctggcttga agaagctggt gagaaaatca aatcttctca
    28201 acaaacatta acaatctcgc aaaaatcaaa tcgaacggat ctagtaacaa atgtcgatcg
    28261 aaacatccag aattttctaa tcgaaaaaat ccattccttt gatccagagg cgaaaatatt
    28321 ggcagaagaa gaagggtgta atcaattatc ggatctcgat ggaagagtct ttatcatcga
    28381 tcctattgat ggcaccttga attttgtgat tgaaggggaa aatttttgta ttatgctcgc
    28441 tgtttatgaa tccggcgttg gaaagcttgg tttcatttat gacgtgatga gaaatgaact
    28501 tttttggggt ggaaaaggaa taggcgtgta taaaaatgat gagccattgc ctagaccagc
    28561 aaataaatcc ttatcggaag gcttacttgg tgtgaatagt tatcttttcg gtcataataa
    28621 atatggaatc cgagaaattg cggaaagcag catggggaca cgtatgtacg gatgcgctgg
    28681 tcttgaacta gtggcaatct tgaaaggaaa ccacattgct tatatttcaa accttagccc
    28741 ttgggattat gcagcgggaa atgtcttatt ggaagagttt ggtatggtaa caagcggtct
    28801 ttttggtact cctttgacat ttgaaaaaag ggaatactat ttagcaggga cttcttgtgc
    28861 atatgcagaa atcgcagacc ttttgaaaaa agaaggctga aaaaaaggta gaaatcattc
    28921 acaaaaatga taaaaacgag caaaagatta attttctcga aaaaatgaaa acattttact
    28981 atatttcatg aaaaaagtct gctataatta gcagtcgagt aaaatttgcc ctgtcataaa
    29041 gaaccaataa aaaaggagtt agttacattg aattatagaa acgatatccg taacgtcgca
    29101 attattgccc acgttgacca cggaaaaaca acattagttg atgaactatt aaaacaatca
    29161 gatacattag acgcacatac gcaattgcaa gaacgtgcga tggactcaaa tgcgctagaa
    29221 aaagaacgtg gtatcacgat tttagccaaa aacacagctg tagactataa aggtattcgc
    29281 gtaaacatca tggatactcc aggacacgcg gacttcggtg gcgaagtaga acggatcatg
    29341 aaaatggttg acggcgttgt gttggtcgta gatgcgtatg aaggaacaat gcctcaaaca
    29401 cgttttgtat tgaaaaaagc actggaacaa catatcacac caatcgttgt cgtaaacaag
    29461 attgacaaac cttctgctcg tcctgagcat gtcgtagatg aagttcttga actattcatc
    29521 gaattaggag cagatgatga tcagttagac tttcctgtga tttatgcatc tgcactaaat
    29581 ggtacgtcaa gcttgtcaga tgatccagca gatcaagaac caaccatggc acctattttt
    29641 gatacaatta ttgaaaaaat ccctgctcct gtcgataaca gcgacgaacc attacaattc
    29701 caagtttcat tacttgacta caacgattat gtaggtcgta tcggaatcgg acgtgtattt
    29761 agaggaacaa tcaaagtagg agaccaagta gcattaatta aattagacgg aacagttaag
    29821 aaattccgtg taacgaaatt atttggtttc tttggactaa aacgtctaga gattcaagaa
    29881 gcaaaagctg gagacttgat tgctgtctca ggtatggaag atatctttgt cggtgaaaca
    29941 gttactcctg tggatcatca agatgcgtta cctatccttc acattgacga accaacattg
    30001 caaatgactt tcttagttaa taattcacca tttgccggtc gagaaggtaa attcgtaacc
    30061 gctcgtaaga ttgaagaacg tttgatggca gaacttcaaa cagatgtatc cttacgagtt
    30121 gaaccaacaa actctccaga tgcatggaca gtttctggtc gtggagaatt gcatttgtct
    30181 atcttgattg aaaacatgcg ccgtgaggga tacgaattgc aagtatctcg tccagaagtt
    30241 attgaaaaag aaatcgacgg tgtaaaatgc gaaccgtttg aacgcgtgca aatcgataca
    30301 ccagaagaat atatgggtag tgttatcgag tccttgagct tacgtaaagg tgaaatgcaa
    30361 gatatggtcc atacaggaaa cggacaaatc cgtttgacat tcttaacacc tgcccgcggt
    30421 ttgattggtt attcaactga attcctttca atgacacgtg gttacggaat catgaaccat
    30481 acttttgacc aatatctgcc aatgcttcct ggtcaaattg gcggacgtca ccaaggggcg
    30541 cttgtttcga tcgatacagg aaaagcaacg acttattcta tcatgagtat cgaagaacgc
    30601 ggtacagtct tcgtagaacc aggtacagaa gtatatgaag gaatgattat cggggaaaac
    30661 agccgtgata atgatttgac tgtcaacatc acgaaagcaa aacaaatgac aaacgtacgg
    30721 tctgctacta aagaccaaac atctgtcatc aaaaaaccaa aacaattgac gttggaagaa
    30781 tctttggaat tcttgaatga tgatgaatat tgcgaagtaa caccagaaag cattcgcttg
    30841 agaaaacaaa ttttagagaa aaatgctcgt gaaaaagcat ctaaaaagaa aaaataatct
    30901 gtaaaaggat gaagagagaa accagcttgt tataaaaaat agctggtttc tttttttgtc
    30961 caactctttt ctgtggagaa gtcatctttg tcaatggaca taaattcaga aaatacgccg
    31021 aaagacgttt aaaagacttt taaaaaactt atttctagag agtttttgtt tcataataag
    31081 ataatgtttc acgaaaggag atcaacttgt ccttcgaaga aaatcgaagt aaaatggcaa
    31141 gagaaaggag cgggaagatg aacgacttat taaaacaata ctttggttat gatgagttta
    31201 gacctggtca aaaagaaatt atccagaaag tcattgatca ggaaaatgta ttaggaatca
    31261 tgcctaccgg aagtgggaaa tcaatctgtt accagcttcc agctttacta ttagatgggc
    31321 ttactgtggt tgtgtcccct ttaatctctt tgatgaagga tcaggtggac gcggcgaatc
    31381 aattaggtat acccgccact tttatcaata gctcgttaga tggttacgaa acagctagac
    31441 gcttccaaga aatcgataga cagcagtacc gtttacttta tatcgctcct gaacgtttta
    31501 tcatgccgga ttttatccaa gcaatgaagc gatggaacgt ccgtatgatc gctattgacg
    31561 aggcacattg tatctctcag tgggggcatg atttccgccc aagttattta cagatggcga
    31621 accagctaga tcaactgcca aatcgtcctg taattgttgc tttgacagct acagcgactg
    31681 tgcaagtagc tgctgacata aaaagacttt taaaaattcc agaaaacaac catattcaga
    31741 ctggttttga acgagaaaat ctacgatttc aagtaattaa agatcaaaaa aaagaacaat
    31801 atctaatcga atatttaaag atcaacaaaa atcaatctgg tatcatttat gcagcaacga
    31861 gaaaagaagt agaccgtctc tatcatttgc ttaaaaaatt cgatttttcc gtgggtagat
    31921 atcatggtgg tctaaatgag aatgagcgaa cagaaatgca agaagccttt ttatacgatc
    31981 gcttgcagct gattgtagcg acaaatgcat ttggtatggg tataaataaa agcaatgtcc
    32041 gttttgttat ccattatcaa attcctggct ctctagaagc atactatcag gaagctggtc
    32101 gggcgggaag agatggtctt tccagtgaag ccatcctgct tttctctcct caagatatcc
    32161 aagtccaaaa gttttttatc cagcagtccc aaagagaaga agggcaaaag caaaaagaat
    32221 atgaaaaatt aaaagcgatg acagaatacg tgtatattga gtcctgtctt cagcaataca
    32281 tcctaaacta ttttggcgag acaagcagtc cgtgcaaccg ttgtggtaac tgtttagatg
    32341 acagagaaat agtagaagta acgacagaag cacagatggt tctttcttgc ttaaaacgta
    32401 tgggtgagaa ctatggtaaa caaatgctga tgaaagtctt agcaggatcg aaagaacaaa
    32461 agctgcaagc tttaggtttt ggacatttat ctacctatgg actgatgaaa aaccaatcgc
    32521 aaaaagaaac gatgcagtta attgaatatt tgatatcgaa tggctatctt ctgaccatca
    32581 atggtgagta tcctgttttg aaagtaacag aaagagggat acaagtattg aaaggacaag
    32641 aatctgttta tcgaaaggaa cccaagaaag tccagcagct atcggatgaa gaaaccgata
    32701 cactatttga agtgctgcgg gaactgcgaa cggatctagc atcagaagca ggcgttcccc
    32761 cgtatgtcgt attttcagat tccacattga aagaaatgag ccgaatacgt ccatcaagca
    32821 gattggagat gcttcagata aaaggagttg gccaaagcaa gcttgataaa tatggcgaag
    32881 cctttttatc tcggataaaa aatgcggact ctaacgttct ggataagtag aaagaacgaa
    32941 tacgtaaaaa tgaataaaaa caactggtta aatctgtttt ttttgctcct tatgaaaatc
    33001 aaatatgtta taattaagga attagaatgg gaggtaagca cagatggatt ctttgtcgaa
    33061 aaaacttgcg atccaagtat taaatgatga agcagaacgg atcaaacgat taatccgaaa
    33121 tcaaaacaac agtctctgta tctcacaatg caaagccttc gaagaagtgg tagatacaca
    33181 gatgtacggc ttttcccggc aagtcggttt tgctgtccgt ataggagtaa tcggaaagaa
    33241 cgagggccag ctgatgttaa gtgagctaga acgagaattg aatcagttat atagtgaggt
    33301 ttaccaagaa atttatgata aaaaagaagt tggcaaggag gaatagactt gcctaagaaa
    33361 gtgaaaaaaa ggcatctatt agactatagc atcttgattc cctatttaat tttatgtgta
    33421 ttgggattga tcatggtata tagttctacg tcgtatctat tattagaaaa cggacaaaat
    33481 ccgtccgcat cagtcattaa ccagagtatt ttttgggtgc ttagcttgat tgctatcgca
    33541 ctactttata aaatgaaaac agatgttttg aaaaaccagc gtttgatcat ggcagccatc
    33601 gcagttttga cgattttgct gctgattgtt gtgttttttg gtaaggagat caatggggcg
    33661 aaaggttggc tgcaaattgc aggattctcg atccaacctg ccgaatacct aaaaatcatt
    33721 tctatctggt acctgtctct gactttatct aaaagacaaa acagtgtaca aaaagatttt
    33781 cttgggactg tgaaacgtcc acttgcaatg gttatcggac ttactgcatt agtcgctatt
    33841 cttcccgatt ttggtaatgc ggcagttatc tttttgatta ttttggttct gcttttagcc
    33901 agcggagtca attatgtgta tactttgatt gtaggagtag gaggcttttg tctaagtact
    33961 tttacgatct ggctgatcaa tatcacaaac ggaaagatct tacccggacg tttacagtat
    34021 atctataatc gatttgctat ttaccaaaat cctttctcag acgaattaaa taaagggcat
    34081 caactagtca acggctacta tgccatgttc aacggaggac tatttggcag gggattaggt
    34141 aacagtatcc agaaaaaagg atttttacaa gaagcacaaa ccgattttat ttatgcaatc
    34201 gtagtggaag aactgggagt gattatgggg attttgatat tagcgttgct attctttatg
    34261 attgtgcgca ttgtccttgt aggtatccgt tcaaaagatc cgttcaattc gcttttgtgc
    34321 attgggatcg gcgcaatgtt tttgatccaa gtatttgtca atcttggagg aattactggc
    34381 gttattcctt tgacagggat cacgttccca tttttaagtc aaggaggctc cagtttgctg
    34441 atgctttcga tttgtgtcgg ctttgtcctc aacatcagtg cggatgaaaa acgcaagagc
    34501 ttaggtttat aataaaaaaa gatgtaggag tggttcgatg aaaaaagttt tagttgctaa
    34561 caggggtgaa atcgctgtac gtgtatttcg tgcttgtaca gaattaggaa ttaaaactgt
    34621 tggaatctat gcagaagaag atgaatattc cgtacaccgt ttcaaagcgg atgaagcata
    34681 tttagtagga caaggaaaaa aaccgattga tgcgtatcta gatattgaag ggatcatttc
    34741 tatcgcgaaa gaatgcggag ctgacgctat tcatccgggt tacggtcttt tgtcggaaaa
    34801 cttgaatttt gcgcaacggt gcgaagaaga aggcatcatt tttgttggac ccaaactgca
    34861 tcatttagat atttttggag ataaaatcaa agcaaaagct gcagctatcg aagcggggat
    34921 tgcttcgatt ccaggcacag atggaccaat cgcttctata gacgatgcct tggagtttgc
    34981 gaaacaatac ggttatccga ttatgatcaa ggcagctcta ggcggcggcg gacgcggcat
    35041 gcgtgtagct cacgatgaaa agagtgcaag agagggctac gaacgtgcga aaagcgaagc
    35101 aaaagcagcc tttggtagtg atgaagtgta tgttgaaaaa tacatcgcta atcctaagca
    35161 tatcgaagta cagattctcg gggatacgca tggaaacgtc atccatcttt ttgaaagaga
    35221 ttgttctgtt cagcgccgtc accaaaaagt agtagaagta gcgccttgtg tctctatgaa
    35281 tgaacagcaa cgtcaaaaaa tctgccaggc agctgtccaa ttgatgaaat atgtcgggta
    35341 tgtcaacgca ggtacagtag aatttcttgt tgaaggagat gatttttact ttatcgaagt
    35401 caaccctcgc gtccaagtgg aacatacgat cacagaaatg attacagata ttgatatcgt
    35461 aacaacgcaa ctgttgattg ctcaaggttt ggatctgcat aaagaaatcg gattgcctca
    35521 gcaagaagga attaaattaa atggttcagc cattcaatgc cgaatcacta cagaagaccc
    35581 gttgaataac tttttaccag acacagggaa aatcgatacg tatcgttcac ctggtggttt
    35641 tggcgtacgt ctggatgtcg gaaatgctta cgcgggctat gtagtgacac cgtattttga
    35701 ttcattgcta gtaaaagtat gtacgcatgg tgctaccttt gaaacagcca ttcagaaaat
    35761 ggaacgttgc ttgaaagaat ttagaatccg tggagtgaaa acaaacattc catttatgct
    35821 caatgtcatc acacatcctg aattccaatc aggaaatgca aaaacaacct tcattgatag
    35881 tacagcaaca ttatttgaat ttcctcgact acgagaccgc ggaaacaaaa cgatgaaata
    35941 tatcggtgaa attactgtga atggttttcc aggtatcgaa agtggcgaaa aaccatttta
    36001 tgaagaacca cgtatgccta aagatctaat cacaagaact gattacgtaa cggctaagaa
    36061 tgtattagat gcgaagggtg ccgatgcttt ggtcgaatgg atcaaaggac aggaaaattt
    36121 gttgttgacg gatacgacat ttagagacgc ccatcaaagt ctgctagcaa ctcgtgttcg
    36181 gacaaaggat ttcaagcaga ttgcacgttt gactggagaa ggtcttcctg aactcttttc
    36241 aagtgagatg tgggggggag caacatttga tgtggcttac cgctttttga atgaagatcc
    36301 ttggcaacgg ttgagaaaaa ttcgttcttt aatgcctaat acgttgctgc aaatgctttt
    36361 cagaggttca aatgccgttg ggtattctaa ttatcctgac aatgtattgg tggaatttgt
    36421 caaagaagca gcagcgcaag gaatcgatgt atttcgaatc tttgatagtt tgaactggac
    36481 tccacaaatg gaaaaaagta tccaagctgt acgggatact ggtaaaatcg cagaggcagc
    36541 tatttgctat actggcgata tcaacgatcc aagtcgggca aaatacaatg ttcagtatta
    36601 taaagacatg gcaaaggaac tagaacaact gggtgcgcat atcattgcaa tcaaagatat
    36661 ggctgggctg cttaaaccgc aggcagctta tcgtttgatt agtgaattga aagaaacgac
    36721 agatctgcca atccatctgc atactcatga taccagcggg aatggaatca tcacttattc
    36781 tgctgcttca aaagcaggag tagacatcgt tgacgtggca atgagtgcaa tgagtgggaa
    36841 tactagtcag cctagcatga gcagcttgta ttatgcactg gtcaatggtc cacggctgcc
    36901 agaaatcacg attgaaaatg cacagaaatt aaaccattat tgggaagatg tgcgcatgta
    36961 ttacaaaccg tttgaaaacg gcttgaatgc tcctgaaaca gaagtatata tgcatgaaat
    37021 gccaggagga cagtattcga atctacagca acaggcaaaa gctgtcggat taggtcatcg
    37081 ctgggatgaa atcaaacaaa tgtatcatac tgtcaacttg atgtttggcg atatcgttaa
    37141 agtaactcca tcatctaaag tagttggaga tatggcacta tttatggtcc aaaatgatct
    37201 tacagaagaa gatatttatg aaaaaggcga aacactaagt ttccctgaat ctgttgtgac
    37261 tttcttccaa ggagaactag ggcaacccgt tggcggattt cctaaaaaat tgcaaaagat
    37321 tattcttaaa ggcagacctg cattgaatga acgtccaggt cttttggctg aatcagtcga
    37381 cttcaacgaa gtaaaaaaag aattggcaga aaaaattggc tatgaaccaa aacaagacga
    37441 agtattaagc tatttgatgt atccgcaagt ctttttggac tatcaaaaag catataatca
    37501 atttggcgat gttacattac tggacacacc aacatttttc caaggtatac gtttgggtga
    37561 aacaatcaat gtacaaatcg aacgcggaaa aacgcttatc attcgtttag atgaaatcgg
    37621 agaacccgat atcgaaggga atcgcgtact gttcttcaat ttgaatggac aaagacgtga
    37681 aatcactatc aaagataatt cgatcatcag tacggtccaa acccgattga aagctgaacc
    37741 gacaaaccga gaacaaattg gagcaacgat gtctggttct gttttggatg tattagtgaa
    37801 aaaaggcgat aacgttaaaa aaggtgatac gttgatgatt actgaagcga tgaaaatgga
    37861 aacggccatc gaagcacgtt ttgacggaga agtcgcacat gtatatgtgt cttcaggaga
    37921 cactatttcg tctggtgacc ttttgattga agtgactgag aaataataag cgaaaggtgg
    37981 ggaaacatga aaagactggg tttgttttgg ctgattctgc tattagttat tgtggcattc
    38041 tacatggaac cagtcttgtt tccgccaaaa cttgaagtga aggaaaactc tgatcaacaa
    38101 atatcaaacc aaatgacaac gtggaaatac caggagctta aagcaactgg tttttctact
    38161 tatatcggaa aaccgatcga tcagctggaa aaggattttg gccaagccta tgatcgatta
    38221 actagcggat ttggttttga aacaagatat tatgaaaata aagctagtca tctcacattt
    38281 gaagcgaaca tccaagacag cagagtcaca actgttaaag tactgcaaac agggcctgct
    38341 gatatcgcgc cgttttattt agggatgacg atgcaagatc ttacgaaaat cactactatc
    38401 tatactaatt tcacttttga gtaccatgat acagaagttg gaatcgaact aatggaagag
    38461 gatatgaatt atcgtccgct aattgctttt gataacgaca cttttgctat tttatttttt
    38521 gatcaaacaa caaacggcct attttcagtt gtgtatttag ataaagatag tcttttgaaa
    38581 ctattgccat atcaagtgtt tggtgaaggc cttccacatt atcaagcaga taaatcggct
    38641 gattgggaat caatcaatcg cgttaaagaa cgaaaaagta cgactttatt aaatatgctt
    38701 agaagagaag acgaacttcc catatacaat caaacacttg cctttcaaaa ccacagtcag
    38761 ctgctactac atgatttttt gacaaggcct gaggaaatat tgtcagagga acgtatggct
    38821 gattggcaaa aatcgctagg cagtgctcaa acgacagtga aattctcact gacgaatgat
    38881 gaattggaga agttaatcag taaacaaaaa ttagaaaata ctagtgggct atttattcat
    38941 ccggcgattg acgctacttt ttcgttttta tattggtaca gcgatcctta caaccatgac
    39001 cgtttcatgg acccgggaac ggattctgta ggcattgctt tttctaaaga aaatatgtta
    39061 gtcttattgc aagaagaaaa cggagaaagt agtgatagca gtgatcgtta acgaagagct
    39121 gtttcaactg gaggatcaat gcctccaagt cgctgaaatg ataaaaaaga gtaaaacgat
    39181 ggcttcttat cttgaacata aacagtcaat ggaagaatct gcaaaagttt gccagttgaa
    39241 agaagcgttc ttgtctaaga aagaagcatt tgaaaatgta gcggcatatg gtgagtatgc
    39301 acctgattat cgtgaaaaaa aacgagctgt tcgaaaagca aaaagacagc ttgatatgga
    39361 tgagcatgtt gcagctttcc gatatagtga aacacagctg caaagtctgt tggatcaaat
    39421 tgtccaagta cttgccgaag aagtttctaa tgaaatcaaa gtagatgcag gtagcccgtt
    39481 ttttgaaacg aaaaaacact ttggatgcgg aggtaattgt catgcaatta gacaatagtg
    39541 aatttgaaat aacaaaacga cgcgggctga tcgtatgggt ctacagctta aaacaattaa
    39601 agaatctgaa aaagttcggc ttgttgcatt atgtctcaag aagaatgaaa tatgtcgtgc
    39661 tttacttaaa tgaagagaca ttggaagaaa cagaagaacg aatccgtaaa ttacattttg
    39721 ttcgaaacgt agaacgttct taccgaccgg atatcgaaat gaattttgcc gaaaaaatag
    39781 gaaacaaaac agtattaaaa gacgaaggtt tcgaaattga agagctaaat acagaaatcc
    39841 gattagctga acatctttaa aaatctctta tgagcagttt ttaaataaaa aataacttgg
    39901 ataacgatcg atttttgatc attgtccaag tatttttttg gtatactagt accagtacca
    39961 tttctaagga gtgagtagat gcggataata tcaggaaatt atggcgggcg ccgtttaaaa
    40021 agtttagcag gggcgaacac cagaccaact acagataaag taaaagaatc catattcaat
    40081 atgattggac catattttga tggagagaca gtattggatc tgtttgcagg aagcggtggt
    40141 ttagctatcg aagcagtatc tagaggctgc agtcatgcgg tatgcgtaga caaaaattac
    40201 caagcgctaa aaatcatcaa agaaaacatc gaaattacga aagaaccaga gaaatttacc
    40261 acaataaaac ttgatgcgga caaagcaatc gaacggctag cagcggagaa acaacaattc
    40321 gactacttgt ttcttgatcc gccatatgca aaacaaaaaa taatcgatca aatccataaa
    40381 atggaagaac tgggactttt ttctaaaacc gcagttatcg tatgcgaaac ggataaaaca
    40441 gtcgaattgc cagaaactgt ggctggattc catcaaataa gaaaacagat ttatggcata
    40501 acggccgtaa ctatctatag aaaagaggga aatcaatgaa acgtgcatta ttcccaggaa
    40561 gttttgatcc ttttacaaaa ggtcacttag atacggtgga acgtgcggct aaattattcg
    40621 atgaggtagt aattggtgtt tttatcaata cttcaaagaa aagtttgttt cccccagaag
    40681 aaagaatgac attgatcaca aaagctgttt ctcatcttcc taatgtaaaa gtcatgcatc
    40741 aagaaaatca attgacggta gaaacagcaa aagagattgg tgcagatgcg ttgatcagag
    40801 gcatacgaag tatcaaagac tttgaatatg aacgagagat tgctcagatg aatcatcatc
    40861 tatatgatga attggaaact gtcttcttat tagcaaaacc agaatatagt cacgtgagtt
    40921 ccagtatttt aaaagaagta ctgcactttg gcggagatgt gagcagctac ttaccgccag
    40981 tgatcaacga agctttagct ggaaagcgtg aatcaaatga aagctaaaca atggttgaaa
    41041 acagtactga ttatcatatt tggaatgttg atcattggat ttgtagtagt tcccttgccg
    41101 tattatgtag aatcaccggg agccacgatc gatctgaaag aactaattac ggtcaatgat
    41161 aaggaagata aaggttctgg gtctttctct cttacctctg ttggtatcag gcaagctaca
    41221 ggtttcttag ctttaaaagc taaaatgacc ccttttgaag aattgatcag tgaagaagaa
    41281 ttgactggtg gggcgacaaa tgaagaatat aatcaaatgc aacagtatta tatggattct
    41341 tcccaaaatg cagcaatcga acaagcgtta aaacttgcag acaagcctta taaaatggaa
    41401 ttcaaaggcg tatatgtcat gagtgtagag aaaacatcga atttctttgg caagctttca
    41461 gttggagata cagtaacaaa agtagacggg aaaagctttc aaagtacaga agcatttatg
    41521 gattatgtaa aaagccaaaa agttggacaa acaatcgaaa tcagttattt gcataatggt
    41581 gaagaaaaaa cagcttctgg tgatctgatc gagttaccaa cagataaaaa ggcaggaata
    41641 ggtatcacgc ttactgacca taccgagatc gaatcagata cagatgttcg tattaattct
    41701 ggctctatcg gtgggccatc agcgggattg atgttcactt tagaaatcta tgaacaagtc
    41761 actggtaaaa acttgcgaca tggtaagcaa attgcaggaa ctggaacgat caacagtgaa
    41821 ggagaagttg gccgaatcgg aggaatcgat aaaaaagtgg caagtgcgga taaagctggc
    41881 gtagagattt tctttgcacc agatgatacg atttcagacg atgttaagaa agaatatcct
    41941 gaaataaaaa ccaactacga agaagcaaaa gctgccgcta aaaaattgaa ttcctcaatg
    42001 aaaatcgtac cagtaaaaac agtacaagaa gcgcttgact atctagagaa aatgaaatga
    42061 aactaaataa ttaaagaggg gttgtgacac gagttctgtc acaacccctc tttttcgtca
    42121 aaaatcttca cgataaaaat aaaaacaaaa gaaaaaattg acgtaattag atgttgaagg
    42181 gacgattttg ccagagctca ctgtttcttc aattttaaat gaaaggagta atgaatgatt
    42241 tttactggtc attctttgaa aaaagaacaa tgatagaaaa aatcagcaag aaaatactag
    42301 cttcacccaa attgatcatt tggatgtcta tgatagttct tatcttttgt atcgcaagtg
    42361 gttttttatt atcaaatttg ctattttata gagaaacaga agagactgct gcaaaagaaa
    42421 tactttcttc gtttagttcc agtggagaag atccatcagc tgaaggaaac gatgtagaag
    42481 aggatgctcg tccaaaggtc atgtacaccg atatcaaagg aagtgtgaag gagcctggta
    42541 tctattcctt ttcttcagaa gaacgggtct atgatgtact aaagcgtgca ggtggattat
    42601 tagaagaagc ggatagcgat cgaatcaact tttctgcgaa gatagaagat cagcaagtcc
    42661 tatatatccc tgctgtaggt gaagagctgc cagaacatct tgatcagttg gcatcctcgg
    42721 aaggaaaaca gcctacagca gattccgagc ctcccaaaat aaatatcaat acagccactc
    42781 catcagaact tcagcagatt cctgggatcg gatcagtcaa agcgcaagaa atcattcgat
    42841 ttagagaaga aaacggatcc ttccagaaag tggaagacct gcaggagatt tctggtatcg
    42901 gtgaaaagac agttgaaaaa ctaaaaaact tcgttacaat taaatagaat gattcaaaat
    42961 cgactggcac gagcaataaa tggacgctaa tggatactaa gaataatcca attctaagag
    43021 cttggcagga cgaggaggta gtatttttgg ataggaaata gcttgattgt tcgcaaagaa
    43081 acattaaaaa aaccaatgct gaatgtctat ctccaaaata aaatatctgg gattcatatt
    43141 atgaatacgg ctgtcagcgg caataacagt caagctctac gagaacgctt tgccaaagat
    43201 gtacttagct atacagcaga taaagtattt attctcattg ggacgaatga tctagcagag
    43261 cataaacaac tttctaaaga aacgtaccaa aaaatctgca gtggatgata gttcagctga
    43321 aaaaagacta tagttctgat catgctttct ttatcacgcc accagcagtc gatgagacaa
    43381 aacaaaaata tcggaataac aagttgatca aagaatatat ctctgttctc acagaagtag
    43441 caaaacgcca tgattgtgaa gtatcgattt ttatacggaa tttcttgaac aaatggattc
    43501 gaagaatttt tataatgggc taatggatga cggacttcat ttcggcagtg aagggtagga
    43561 tcttttgtca gacaggatga ttcgttatat ttaaagaata ttttgtttaa gcaatgagta
    43621 ctttttaatc aacttgaaaa aacggaggaa tataaaacat gttagaaaaa tcttcaaaag
    43681 catgcgatac gaataaagaa aaagttactg ataagtcaca taatgaacgc attccttggg
    43741 accagtactt tatggctcag gcagtactgt tatctttacg tagtacgtgt acacgtttag
    43801 aagtaggagc gacactcgtt cgagaaaaaa ggatcattgc tggcggctat aatggagcag
    43861 tcagtggaga tgtccactgc atagatgaag ggtgttacgt cgtggatgga cactgcttgc
    43921 gaacgatcca tgcagagatg aatgcactcc tacagtgtgc aaaacttggt atcccgacgg
    43981 aaggttcaga aatctatgtc acacattttc catgtttggc ctgtacaaag gctttgttgc
    44041 aagctggtgt gaaagggatc aattatctcc atgactatcg aaatgatcct tatgcccaag
    44101 cgttgatcga acaattaggt gtatctgtac accaagttgc tttagactca cagcattttt
    44161 caaaacttca aaatgaatta acaggaaaac atgtctagcg ttttagaaag gcatgttcga
    44221 aatcggttga tttttccggc aattacgtcg atgattatct gtttgttgta ttacactgac
    44281 aattggggtt atcgtttgct tcttgccgga atttttttat ggttttcgct aaaagaagga
    44341 cgttttatcg cgtacttgtg tattttgtgt agcataacgg tatccatttc ttataggcta
    44401 caagagtatt catttttaga ggaaaaaaca acatctgcgc aaattactgt atttacagat
    44461 acgattcgtg caaatggtaa ttttttgaca atggaaggaa ggttatcaga gaataaacaa
    44521 aagctccagc tttcttatca agtaaacaca gaagcagaat tgaaaacatg gcttaacttc
    44581 cgaggaaagg gaatagaatt gactgctgaa ggcgagttca tctctccagc aaaaaagcgg
    44641 aatctttata attttgacca agaagcttac ttcaggaacc atagaatttc tggacggttc
    44701 caaattcaaa agattgaaca gatcaccatg caaaatcact ggcgaaactg gcttcagaga
    44761 aaaagaggat cttttatctc ctttgtccaa tctgaatttc cgcaaaaaac aagtgtatat
    44821 attaattctt tattatgggg ctataaagat gcaggattca gagaaccaga agaaattttt
    44881 agggagagtg gtttgctgca tctgttcagt ctatcgggtc tacacatcca attttattta
    44941 gggtggctgt attatttatt tcgacgtatc ggatggccac tacacgtcag tattattccg
    45001 ttgagtctac taactatctg ttatgtttct attgcaggaa gttctatttc tgttttgcgt
    45061 gctagtttgc tgtttttatt gcgattgcta gtcaaattgc tgaataagca ttattccacc
    45121 atggacttat ttgcagttgt cttttggctt cttttactat ttgatcctcg atttcttttt
    45181 catgcgggtg gacagcttag ttttttcatg agttttttgt ttatcctgat ttcttttgat
    45241 ggagattggt tgaaaaaaac ggcctctcac gtcctgttta ctatgatcac gatgccgttg
    45301 attgtatggc atttttatga gtggcctatt ttaggtagtc tttttacatt actgattgca
    45361 ccggtattca agtttctttt tcttcctggt gtgttatttc tcacactctt caatcgctgg
    45421 ctgccggaaa ctttactttt gctgatagaa gagggattaa tcttatttga gcgaataatc
    45481 gatttttctt cagggtcgtc tctcattata ggacgtttgc cccttgtgct atctgggatg
    45541 tatattttcg gattactgat ttgtatggat aggttgaaag aacaacctgt gaaacacttg
    45601 ttttatgttg gtcttttttc ttttattttt ttgactttgc catttttacg cttttcttca
    45661 acgatcgctt ttatcgatgt cggtcaagga gacagtatct ttttgcaaag tccttttcga
    45721 aaagaaacga ttttgatcga tactggaggt cagttacact ttgaaagaga aaagtgggcg
    45781 caaggtatga tacgtcctcc tgctgaaaga aatatcgttc catatctaaa aggtcagggg
    45841 ataagtgtaa tcgataaact catcttgaca cacgacgata cggatcatgt gggagaatta
    45901 cccacgttat ctcagcattt tactatcaaa aaaatttata tcggttgggg cgctggacga
    45961 aatgagccat tacaaaggca tttatctgaa gctcaaaaaa atggtactga gatcattgaa
    46021 atcaaacatg gggacaggat cagcggttat tatgatcttt atgtattgac tccttttgaa
    46081 aagggagagg gaagaaatga agattcgatt ggtctatgga tggaatataa caataggcgc
    46141 tttttatttt taggagattt gaatcaagca atggaaaaac aattgctttt gatttatcct
    46201 aatctgaaag ccgatgtagt caaattaggt catcacggta gccgtacgtc aagtaacaca
    46261 gattttcttt cccatattga agcaaaacat gggattatat cctgcggcgt aaataatcgt
    46321 tatggtcacc cgcacagcga agtattggag acactagaag aaaatcagat aaaaacatgg
    46381 cgaacggatc aacaaggaat gatcacctat cgctggcatc cagtttttca tcctcatggg
    46441 ctagtggaaa caatgataga ctagtaattc tcatctgctc atggtaggat aatttttgaa
    46501 agtggtgagt aatttgagta tacaagcaga gttacaaaaa atacgtaaag aaaaacttgc
    46561 atcttgttac ttgattttag gaacagaaaa gtttctccaa gatcaagttc ggacagaaat
    46621 tgtcaaaaaa atccaattag agggcgaaga tgacttaaat tttttaagtt ttgacatgga
    46681 aaatgcaagt ttagatgagg tagtagctga agcagagacg ttgccgtttt tcggagatca
    46741 acggttagta ttcgtagaga atccttattt tttaacaggt gaaaaagtaa acaatggaat
    46801 cgaacaaaat actgatttat tgacggctta tttgaaagaa ccattagaat cgactgtatt
    46861 agtattcttt gcgccatatg aaaagttgga tgaacggaaa aaagtaacga aacaattgaa
    46921 aaaaacagca attatcatca atgtccaaca gttgaatgaa aaagaagtac gtcaatatct
    46981 gatgaatacc ttagagaatt cctcaatcaa aatggaacga agtgcaattg atttattctt
    47041 gaggttgacc gacttagatt tatccaaaat gatgagagag ttacagaaat tggtccttta
    47101 tggacaaaat caacagcaaa tcacggtaag agaagtagaa gaattagttc ctaaaacgtt
    47161 agaacataat atttttgaca tgacccaata tattttaaaa gggaagacgg aacaagcttt
    47221 gcgtttatac gaagatttag ttatgcaagg ggaagaaaca atcaaaatta atgcgatttt
    47281 gctttctcaa ttacgtttat ttttacagac aaaatttctt gtaaagattg gttatcagca
    47341 ggcaaatatt gctgaaacat tgaaaatcca tccatatcgt gtaaaacttg ccatgcagga
    47401 agtaaaaaaa ttcgatgaac atttgctggt acagttgttt gatcaattag ttgagatgga
    47461 ttatcaaatc aaaacgggac aaatcgaaaa agaattaagt tttcaactat tcgttttgcg
    47521 cacagggcag ttgttaagaa atcgataaga agaaagacga gggcaaagca tgcgggaagg
    47581 tcgtttgttt ttgtcctttt tttgacaaat aaaaaaatcg gccgaagccg attcttttat
    47641 ttagcgattt ttttgcttaa acgagatttg tcgcggttag ctttgttttt gtggattaga
    47701 ccttttgatt cagccatatc aatagcttta acagcttctt tatataacgc atctacgttg
    47761 tcagcacctg aagctacagc atcttcaaat tttttgattg cagtacgcat agcgcttgtt
    47821 tgagatgaat ttttcacatt agcgttttcg cttgtacgaa cacgtttgat tgcagattca
    47881 atgtttggca ttagtttcac ctccgataac ttaagttctt acgcgtgtta ccgcataatt
    47941 caacattcat tattatacct aaaggcagcg tgctttgcaa tatgaacatg acaagttttt
    48001 ttccttaaca gtcttttatt aggatttttt ttcagtaaaa acggatagta caagctgaaa
    48061 aa
//
LOCUS       DANKAZ010000012        45400 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000012 DANKAZ010000000
VERSION     DANKAZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(158..643)
                     /locus_tag="PWY67_000632"
     CDS             complement(158..643)
                     /locus_tag="PWY67_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL2056940.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    733..1078
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1123..1764
                     /locus_tag="PWY67_000633"
     CDS             1123..1764
                     /locus_tag="PWY67_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2056941.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1783..2418
                     /locus_tag="PWY67_000634"
     CDS             1783..2418
                     /locus_tag="PWY67_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2056942.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2436..3251
                     /locus_tag="PWY67_000635"
     CDS             2436..3251
                     /locus_tag="PWY67_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2056943.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3570..5006
                     /locus_tag="PWY67_000636"
     CDS             3570..5006
                     /locus_tag="PWY67_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2056944.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5195..7642
                     /locus_tag="PWY67_000637"
     CDS             5195..7642
                     /locus_tag="PWY67_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2056945.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7872..8939
                     /gene="rlmN"
                     /locus_tag="PWY67_000638"
     CDS             7872..8939
                     /gene="rlmN"
                     /locus_tag="PWY67_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2056946.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9132..10235)
                     /locus_tag="PWY67_000639"
     CDS             complement(9132..10235)
                     /locus_tag="PWY67_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2056947.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10788..11336
                     /gene="aac(6')"
                     /locus_tag="PWY67_000640"
     CDS             10788..11336
                     /gene="aac(6')"
                     /locus_tag="PWY67_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2056948.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11882..13543
                     /locus_tag="PWY67_000641"
     CDS             11882..13543
                     /locus_tag="PWY67_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2056949.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13613..13960)
                     /locus_tag="PWY67_000642"
     CDS             complement(13613..13960)
                     /locus_tag="PWY67_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL2056950.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            14044..14985
                     /locus_tag="PWY67_000643"
     CDS             14044..14985
                     /locus_tag="PWY67_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2056951.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            14986..16029
                     /locus_tag="PWY67_000644"
     CDS             14986..16029
                     /locus_tag="PWY67_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2056952.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16035..17105
                     /locus_tag="PWY67_000645"
     CDS             16035..17105
                     /locus_tag="PWY67_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2056953.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17095..18048
                     /locus_tag="PWY67_000646"
     CDS             17095..18048
                     /locus_tag="PWY67_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2056954.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18060..19070
                     /locus_tag="PWY67_000647"
     CDS             18060..19070
                     /locus_tag="PWY67_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2056955.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19355..20176)
                     /locus_tag="PWY67_000648"
     CDS             complement(19355..20176)
                     /locus_tag="PWY67_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2056956.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20273..20551)
                     /locus_tag="PWY67_000649"
     CDS             complement(20273..20551)
                     /locus_tag="PWY67_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056957.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20819..22828
                     /gene="metG"
                     /locus_tag="PWY67_000650"
     CDS             20819..22828
                     /gene="metG"
                     /locus_tag="PWY67_000650"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL2056958.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23307..23894)
                     /locus_tag="PWY67_000651"
     CDS             complement(23307..23894)
                     /locus_tag="PWY67_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056959.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            24069..25391
                     /locus_tag="PWY67_000652"
     CDS             24069..25391
                     /locus_tag="PWY67_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2056960.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25546..26322
                     /locus_tag="PWY67_000653"
     CDS             25546..26322
                     /locus_tag="PWY67_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2056961.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26315..26884
                     /gene="rnmV"
                     /locus_tag="PWY67_000654"
     CDS             26315..26884
                     /gene="rnmV"
                     /locus_tag="PWY67_000654"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL2056962.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26877..27761
                     /gene="rsmA"
                     /locus_tag="PWY67_000655"
     CDS             26877..27761
                     /gene="rsmA"
                     /locus_tag="PWY67_000655"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL2056963.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28212..28841
                     /locus_tag="PWY67_000656"
     CDS             28212..28841
                     /locus_tag="PWY67_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056964.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(28965..30074)
                     /gene="ugpC"
                     /locus_tag="PWY67_000657"
     CDS             complement(28965..30074)
                     /gene="ugpC"
                     /locus_tag="PWY67_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL2056965.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            30283..30705
                     /gene="mgsA"
                     /locus_tag="PWY67_000658"
     CDS             30283..30705
                     /gene="mgsA"
                     /locus_tag="PWY67_000658"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL2056966.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            31028..31564
                     /locus_tag="PWY67_000659"
     CDS             31028..31564
                     /locus_tag="PWY67_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2056967.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(31604..33367)
                     /locus_tag="PWY67_000660"
     CDS             complement(31604..33367)
                     /locus_tag="PWY67_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2056968.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(33370..35085)
                     /locus_tag="PWY67_000661"
     CDS             complement(33370..35085)
                     /locus_tag="PWY67_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2056969.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(35864..36088)
                     /locus_tag="PWY67_000662"
     CDS             complement(35864..36088)
                     /locus_tag="PWY67_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056970.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            36279..39056
                     /locus_tag="PWY67_000663"
     CDS             36279..39056
                     /locus_tag="PWY67_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2056971.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            39137..39556
                     /locus_tag="PWY67_000664"
     CDS             39137..39556
                     /locus_tag="PWY67_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL2056972.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            39568..40038
                     /locus_tag="PWY67_000665"
     CDS             39568..40038
                     /locus_tag="PWY67_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2056973.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            40055..40834
                     /locus_tag="PWY67_000666"
     CDS             40055..40834
                     /locus_tag="PWY67_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2056974.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            40821..41642
                     /locus_tag="PWY67_000667"
     CDS             40821..41642
                     /locus_tag="PWY67_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2056975.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            41659..42708
                     /locus_tag="PWY67_000668"
     CDS             41659..42708
                     /locus_tag="PWY67_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2056976.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            42721..43725
                     /locus_tag="PWY67_000669"
     CDS             42721..43725
                     /locus_tag="PWY67_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2056977.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            44358..45377
                     /locus_tag="PWY67_000670"
     CDS             44358..45377
                     /locus_tag="PWY67_000670"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2056978.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        14882 a         7947 c         9411 g        13160 t
ORIGIN      
        1 atatggattt ggctaactat ggaagaatac tcgtattagt tttcagcgtt catactagtt
       61 gttacattgt ttgattggcc aaaagaattt ctgatatatt ttgtgctaaa taaagaaaaa
      121 gacgcttgaa ttgggaaata agaaaactac tctatcatta caaattttca taaactggca
      181 gatgttctaa gtaaacaagt atcttttcaa agtcggaaaa atccataatc aacgtagtgg
      241 tattgatatt tggatgaaag cctacggtat cttgacgatc aatagcagca tcgatcacga
      301 cttggatttt tttctctttg tcgtgcatca atgctaatgg agtcactgta cctgctggaa
      361 cgcctaggag gtcaggaagc tgttcttcag aaacaaaaga taagcgcttt tcttttaatt
      421 gttcagctag ttgttttaaa tcggcgtgct tttcatcagg caagatgaca agatagattt
      481 gttttccttt tcttgatttc aataatagat tttttacttg cggaccaggt agggaaaaag
      541 ggacattttt gactgacgta atcgcaggat gatcgacttt ttgaaaagag atacccagtt
      601 tttttagtag ttcataagct tcttgttcgg ttgcacgact catcgcgcat caccctcctt
      661 tttttattag tgtatcataa aaaagagttg acaaatgaaa aataatcatg taaattacac
      721 gtaaagttaa aacaaatgac atcgaaaaga agagtagccg ttaggtgttt ttagagagtc
      781 tctggttggt ggaaagaggc agcagttaac cgtgaaacac atcttggagt cgattttctg
      841 aagcgtatag taggaaaatc cggggagccc gttacagctg cggtcgttgc tagatgacca
      901 gataaggctg gcttttccgt gaggagtcgg taaaaaaagg tggaaccacg tgattaacgt
      961 cctttgatct tcggatcaaa ggactttttg ttttcatctg aacggcaacg actgtgtgag
     1021 gttttttctg caaggaaaaa ataaacagag gtggaaccac gtataatacg tcctcttggc
     1081 atttgccaag aggatttttt tattttaaag gaggaaaaga agatggagta tttgattgaa
     1141 gttatgccgc aattattagt aggagcactc acaacattga aattattttt atttaccttg
     1201 attggctcat taccattagg gattgtttta gcattaggat tgtctttgca ttttaagccg
     1261 ctacgctact tgcttcaaac atatgtatgg gttatgcggg gaacaccgct gttgttgcaa
     1321 ctgattttta ttttttatgg gttgccgacg attgggatcg tttttgatcg tttcgaagca
     1381 gcactcattg cctttatctt aaactatgca gcttattttg cagagatctt tcgtggaggg
     1441 atacaatcga ttcctcaagg tcaatatgaa gcagcgcaag ttttgcaatt aagtcccttt
     1501 caaacggtga cgaaaatcgt catcccacaa gtgacaaaga ttgttttacc ttctgtaggg
     1561 aatgaagtta tcaacttggt caaagattct tccttgatct atatattagg tttaggtgac
     1621 gtaatgcgtg ctgggaaaat cgcgatggaa cgcgacgcga ctttactgcc tttgctaggc
     1681 gtggcaatca tctatcttac attgactggt attctaacaa tcataatgaa acaagttgaa
     1741 ggaaaattaa attattaccg ctaactgata ggaggaagaa atatgattga attgaagaaa
     1801 gtgaacaaag catttggtga aaaaaaaata atcaaagatt tgaatctaat cattccagat
     1861 gggaaaacat tagctatcgt aggtccttcc ggtggcggta aaaccacgct tttacgtatc
     1921 ttggctggct tagaaagttc tgacagcgga gaattttatt tagacggtgt accgttcaac
     1981 ccattcgagc ttaaaatgaa agaacaagtt gtaggagtcg tattccaaga cttccagcta
     2041 tttccacatc tgaatatttt tgaaaacatt accttagcac caaaaatggt attgaaacag
     2101 acaaaagaag aatacaccaa aaaagcagaa gaattagcgg cttcattagg gattagtgat
     2161 ttattgaata tctatcctta tcagctttct ggcggacaaa aacagcgggt agcgattgcc
     2221 agagcattgg ctatgaatcc acgggtattg gcttatgatg aaccaacaag tgctttagat
     2281 cctgaattac gccagcaagt cgagcgtttg atcctatcat tgaaagaaga tggggtaacc
     2341 caaattattg tcacccatga tttagtattt gcagaaaatg tcggtgatca attactaaaa
     2401 gtttccccaa ttcattaaga aaggagaaga tagagatgaa aaaaaccagt tttctattcg
     2461 ctttgatcgc aggtcttctt ctttttgcag gctgttcgaa caaaaaaact tcagcagata
     2521 attgggcgac tatcaacgaa gagaaaaaaa tcgttatagg tttagacgat acatttgttc
     2581 ctatgggatt tagagataaa gacgggaagc tcacaggatt tgatattgat ttagccaaag
     2641 cagtctttga acaatatggg attacggtag actttcagcc cattgactgg tcaatgaagg
     2701 aatttgaact gaacaatgga acgatcgatt tgatatggaa cggctattcc aaaacgccgg
     2761 caagagaaaa gaaagtgcaa tttacaaaac cttatatgga aaacgatcaa gtgctgatca
     2821 cacctaaaga atcaggaatc acaaactttg gacaaatgaa taataagaga ttaggtgcgc
     2881 aaaatgggtc atctggttat gatgtattta cgaaacaacc agaagtatta aaagagatag
     2941 tcaaaaacca agatgctgtt ttgtatgata gttttaatga agcattgatc gatctgaaaa
     3001 gcagacgggt cgatggtctg ctgatggata aagtatacgc agactattat ttgaaacaaa
     3061 gaaacgaact agaagacttc aacataacaa aaggttctta tgagagtgag gattttgcag
     3121 ttggtgcacg caagtcagat agtacattag tgaaaaaaat caatgaagcc ctagatcaac
     3181 tagagaaaac aggtgaattc caaaaaatat ccgataaatg gtttggcgaa gatgttacgc
     3241 cgagaaatta acaaataggg gactgtgaca aaagacgtgt cacagtccct ttttccgaat
     3301 aaacagtgtt ctcctgccaa ctccttgtga ctcggagctg aatggcaggc tcacaacctc
     3361 ttggtaaacg gtgttcaaaa gccagttttt cggtattaag ccaaatttat gcaaaaatgt
     3421 agaaagccat tttcacaaaa tttttcttac tactcgaaac taagcgactt tttcacaacc
     3481 tcctttacat aattttattt tgttgtggaa ggggtttcgt gttattctaa aagaggaacg
     3541 attgaaggtt ataggaaagg aagagatgaa tggaacacaa agaacttacc tcatttccaa
     3601 aagattttct gtggggctct gcttctgcag cgtatcaagt agaaggagct tggcaagaag
     3661 atgggaaagg agaatcagtc tgggatcgat ttgtacggat acctggaaag acatttaaag
     3721 gaacgaacgg tgatttggca gtagaccact atcatcgtta taaagaagat attgctttaa
     3781 tgaaagaaca aggtctgaaa gcttaccgct tctctgtagc ttggacacgg atttttccta
     3841 atggacgagg agaagtcaat caagctgggc tcgcttttta tgagagattg atcgacgaat
     3901 tgattgaaaa cgatattgaa cctatgttga ctttatatca ttgggatctt ccacaagcat
     3961 tgcaagatga atataatggt tgggaatcac gtcagatcat tgaagatttc actcattatg
     4021 cagaaacatt atttgaggca ttccgcggga aagtccatta ctggattagt ttgaatgaac
     4081 aaaatatctt cacttcatta ggttatttgt tagcagcaca tccgccaggt gtgacagacc
     4141 cgaaacgaat gtatgaagtt aaccatattg ctaatttagc aaatgcatct gtcatcaata
     4201 aatttcacga gttagaaatg cctggaaaaa tcggaccaag ctttgcgtat acgccaaatt
     4261 atccaattga cagtaatcct gaaaatgttc tggctgctga aaatgcagaa gacctgatgg
     4321 ccaattattg gatggatgtc tatatgtggg gaaaatatcc gattgctgcc atgaggttct
     4381 tagaagaaaa aggatgggca ccaacgattg aagctggcga tgcagaattg ctagaatctg
     4441 ctaaaccaga ttttcttggg atcaattact atcaaacggc gacgaatgca ttcaatccat
     4501 taaacggagt tggtgctgga aaaatgaata cgacaggaaa aaaaggttca tcagaagaaa
     4561 ctggagttcc tggaatgtat aaaaaagttg agaatccatt tgtcgaacga acaaattggg
     4621 actgggagat tgacccagaa ggattaagga tcggactgcg tcgtatcaca agccgttacc
     4681 gcatgccggt actgattacg gaaaatggct taggagagta tgacaagttg acagaagata
     4741 aacaaatcca tgacgactat cgaatcaatt acttacaaag ccacgtcaaa gccatcaaag
     4801 aagcaatttc ggatggagca gaagtcctag gctattgcac ctggtcttat acagatttgc
     4861 ttagctggct gaacggttat caaaagcgat atggatttgt atatgttgat caagacgaaa
     4921 cacaagaagg atctttggaa cgatataaga aagacagtta ttactggtac caaaaactga
     4981 ttgaagaaaa tggagagaac ctttagttca catacttaaa acaagaaggt acggctggat
     5041 atggccgtgc cttttcttga taaaatcatt tagaaattaa gaaaaactgt aaaataagct
     5101 gaaaaatgaa agaaagcgtc agttctttca aacaaaactt tagttccctt actttttttt
     5161 gtataatgaa acttgatgaa atgagggata ataaatggca caaaagaaac ggcctactaa
     5221 aaaaaggaaa aagacgaaaa aaacgaaaaa gcaaaaacag cagcaggaac aaatgctgac
     5281 gattggtgtt gggtttgcct tgattttatt tgctgtcttc ggctttttaa aattaggttt
     5341 tttaggaata ttgattgcaa acggctttcg gatcattgca ggaaatactt accaaatcct
     5401 ctgtctttta ttagccgtat taggattttg gattgtcatt aagaatacag agttttcaat
     5461 tggtaaaaac cgacgctggt ttggcggcat actcttttat ttcggtatct tattattgtt
     5521 acatgcccat ttatttggca aactccatac tggggaacca aatatcatgg ggacgacatg
     5581 ggatttgtta gccagtgata taaaacaaag tcaagtagac aataacgttg gtggtgggat
     5641 gattggagca atcctttatc attttactta cttcttgatt gcacagcctg gaagttattt
     5701 agcagcagtg ctattactgg caggaggagc attcctgttc agtaatctgg aaggctatca
     5761 gctgttaaat ggtatccaat caattggaga acgagtacag gaattattgg aaggtgatcc
     5821 tgccaagcaa gctcgaaagc aagcggcaaa ggaagagaga atgaaacagc gcgcagaagc
     5881 gaaagaagca aggcgcttag ctgcaaaaga agctgcagaa aaagaagctg cagaatatga
     5941 gaagaaaaaa gcggtgagcc agcaaaggga aaatactgct gatgaatggc aggaaccaga
     6001 aaatacagag ccggagcagc taagttttgt tccaatcgac agttttcaag aaaatatcca
     6061 tcctgcgaat ctagagaaac cagtaccaga tacaccaaaa cagaccaata cagcagaagg
     6121 tttttcagat gagttgccag aggatgacgg tacctcatta gaatttgaga ttgaagcaga
     6181 acaggaaaat caagactatg aattaccgac agttgatttg cttgattcta taccaactgt
     6241 ggatcaaagt gatgagtata aaaaaattga aaaaaatatc ggcgtattag aacaaacttt
     6301 ccaaagtttc ggtgtcgatg ccaaggtcgt caaagccagc ttaggaccat ccgtcacaaa
     6361 attcgaagtc cagccggctg taggggtaaa agtaagtaaa attgtgaact taacagatga
     6421 tatcgcgctt gctttagcgg cgaaagatgt ccggatggaa gcaccgattc caggtaaatc
     6481 actgattggt attgaagtac caaatggaaa gatcagtatg gtttccttta gagaaatcat
     6541 cgaagcacaa ccaaaccacc cagataaatt actagaagta cctttaggac gtgatgtttc
     6601 tggacgggta caaacggctg atctttcgaa gatgcctcac ttactcgtgg ctggttcgac
     6661 tggtagtggg aagtcagtag caattaatgg gattatcaca agtatattaa tgcgtgctaa
     6721 gccccatgaa gtaaaactga tgatgatcga tccgaaaatg gttgaattga atatgtacaa
     6781 cggcatacct catctgttga cgccggtcgt gacgaaccct agaaaagcag cgcaagcctt
     6841 acaaaaagta gtacaagaaa tggaagaacg atatgaaaaa ttcgcggcaa ccggagtaag
     6901 aaatatttct ggttacaatg aatttgttca acagaaaaac ttggaaaacg gaacgaaaca
     6961 tccaacctta ccattcatcg tagtgatcgt agatgaatta gctgatttga tgatggtagc
     7021 cagcaatgaa gttgaagatg cgattattcg tttagcgcaa atggctcggg cagcggggat
     7081 tcatatgatc ctagctactc aacgtccaag tgtggatgtt atcacaggaa tcatcaaagc
     7141 aaatgtacct tccagaatgg catttgcggt atcaagcggt acagattctc ggacgatcat
     7201 tgattctaat ggggcagaaa agctcttagg gcgaggagat atgctattct tgccaatggg
     7261 tgagaataaa ccgatccgtg tgcaaggagc attcatttct gaccacgaag tagaacgggt
     7321 cgttcaattt gttacagatc agcaagaagc tcattatgaa gagaaaatga tgccgacaga
     7381 tgaagttgaa acgacaggtg ctccagaaca gccacaagat gagctgtttg aagaagcaaa
     7441 agcacttgtc gttgaaatgc aaacggcaag tatttccctt ctccaaagaa gatttcggat
     7501 cggttacaac cgagcagcac gtttagtaga tgaactggaa gctcatggag ttgttggccc
     7561 gtcagaagga agcaagcctc ggaaagtatt cattgagcag gaagaaacat cagaaacgtt
     7621 gccggacgat aatgacatgt aaaacttgtg tgatgaaggg ttgaaaaacg aataaccaat
     7681 aaaaagacaa ggcatgatga taccaaaaga aaaaatggtt tttttccatc ttttctaata
     7741 cggtatgatg tcttgtcttt ttagtttttg tgagattaaa gttctattcg atttatttgt
     7801 gaagtattgc attaaggaaa tgaatcatgt atagtatgta gcgaaacttt gagaaaaaga
     7861 gggaaaatac tttggaaaaa cagtccattt atggcttgac gaatgaagaa ttaatcaatt
     7921 ggtttataga aaacggcgag aaaaaattcc gagctgccca agtttgggaa tggttgtatc
     7981 aaaaacgtgt aagtaatttc acagaaatga cgaatctatc gaaacaattg atcgaaaaat
     8041 tatcggagca ttttatcatc aatcctctaa aacagatggt cgtccaagaa gcttcggatg
     8101 gtacagtaaa atatttgttt gaactccctg ataaaaacat gattgagact gtactgatga
     8161 gacaagaata tggcttgtca gtttgtgtga cgacacaagt aggatgtaat atcggctgta
     8221 cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct gactgctgga gaaattgttg
     8281 cacagatcat gatggtccaa cattattttg atgagcgcaa tcttggcgaa cgcgtttcac
     8341 atgtcgttgt aatggggatc ggtgagccat ttgataatta cgacaatgta atggactttt
     8401 tgcatatcat caatgatgcc aaaggattgg caattggtgc tcggcacatt acagtttcca
     8461 ctagtggatt agcacacaaa atcaaggaat tcgctaataa cggcttgcaa gtcaatctgg
     8521 ctatttcctt gcacgcgccg aataatgagg tgcgaacatc gatcatgcgg atcaaccgaa
     8581 gtttcccaat tgagaaattg atggaagctg tggatgagta tttagagaaa acaaaccgtc
     8641 gaatcacgtt tgaatatatc atgttgaatc aagtaaatga tagaccagag catgcgcaac
     8701 aattagcaga tctattaaaa gataagaaaa aattagcata tgtcaacttg attccatata
     8761 atccagtcag tgaacatgat caatattctc gcagtccaaa agctgatgta ttgagatttt
     8821 atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg taaagaacat ggtacagata
     8881 ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa aaaaacagtt gccaaataat
     8941 cgtatcctac tcgttttttc aattaccata aaaggagcag ttttcttaat taaggaactg
     9001 ttcaacttaa ataagagaaa agattctata gcgaaagcta taaagctttt tttgacaaga
     9061 aaaaccctca tcaagcaaag aaatgtcctc aatttgatga cagcctgctt gatgggggtt
     9121 ttagcaatta tttaactgat tttcttatat ccttgctggc tttcttgtgt agcatgataa
     9181 gtttctacgt caagaatggc ctgtttaaat ttttctcggg tgatttcata aggcacatgt
     9241 ttaatatcat ttgttgccat ggctttatct aagattgctt ctaaatcggt cgtgacattg
     9301 agattcatgt cggttaacga tactggaagt tggttctttt tcataaacac atagatttta
     9361 tcaaacaaat cgaactgctg atccatcatc aataaggtca gtacaccata actgacagat
     9421 gctccgtgaa ggtatccctc cattttttct agaacagtcg taccgtaata gaatgagtgt
     9481 gccatagcag aattatagtt attgttaact aaaacagaaa ccaatccggt agtaacaatg
     9541 atatccgaaa cgacttgttc aattgcatat gagggacgat cgtcggcact atctttcaat
     9601 gcttgcgtac cataagtcaa gagacgatca atgcacattt ttccgatttg gattcctaat
     9661 ctatccgtgt agcctagatc atcgccacga gcagaaaaag tagattcgac ttctttggat
     9721 aaagcatcgc caatacctgc ccataagtat ttatcaggtg cttcggcaat aattgctgta
     9781 tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga gtccgtccat tacgccttct
     9841 tcgttataga tgacacatac cgcagtgacg ggcgaacagt tcgaaccaat agtgggaaag
     9901 gtgaagactg gtttagctag tttttcacca atgactttta cagtatcgca agcacggcca
     9961 ccaccgactg caaacaggac gtctgctttt tggacacttt catgctggat caactgttca
    10021 atatttccat aagttgaatt acccccgtac cagagactgg cgacaacctc tatttcctgt
    10081 gtcaaagcta cttttatttt gttttctgcc tttgaaagtg cagtcttccc tccaatcagc
    10141 actacttttg tcccatattc tttgacgatt ccatttgctt tttcatacgc atctgttcca
    10201 atggtataat ttggtaagaa aacactttga ctcatattca tttccttctt tcttcgttga
    10261 aacgggttaa aattttaaag agtgatcgat ctgactgcgg ctgtagatgg agtagaggtg
    10321 aacctaagac aggataagtg atgaataagg tggcatgaac accttgaaat catcaataag
    10381 tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac aataatgaat ttttgttcct
    10441 cttagtattt ctaccgattc cctttgcttt tttgtaatta tctaaaaatt ctaaaaatta
    10501 agattattat atgaaaacag gttttatttg tcaattcata attggttttt tttaaagcaa
    10561 aagataatct atatcatgat gaacaaacag agttttttcc tccaattgat ggctttcctg
    10621 atctttccta ttttgaataa agtgtttctg tataattttt ttattttata agtaagataa
    10681 ataatgaaag aatagagata ctgattggta gctaaagatt tttgagcgag aacatttcgt
    10741 tttttcaaaa ggactgttag aataagcaag aacaaggggg aagacgtatg ataatcagtg
    10801 aatttgaccg taataatcca gtattgaaag atcagctttc tgatttactg agactgactt
    10861 ggccggaaga atatggagac agctcggcag aagaagtaga agaaatgatg aatccagaac
    10921 gaatcgcggt agcagcggta gaccaagatg agttagtagg atttattggt gcaatccctc
    10981 aatacggtat cacaggttgg gaattgcatc cattagttgt agaaagctcc cgacgaaaga
    11041 accaaatagg tactcgatta gtcaattact tagaaaaaga agtagcttcc agaggaggaa
    11101 tcacgattta tttaggtacg gatgatttag accatggaac aacgttaagt caaacggacc
    11161 tgtatgagca tacatttgat aaagtggctt ccatccagaa ccttcgtgaa catccgtatg
    11221 aattctatga aaaattaggt tataaaatcg taggtgtctt accaaatgca aatggctggg
    11281 acaaaccgga tatttggatg gcaaaaacga ttattcctcg accaaattct caataataaa
    11341 aagaggttgt gacagaagta atcagcttca agaaataaga aggaatttac gaaaattact
    11401 tcttgaattt ttgtgaaatt ccaacttatt tccgaagaag ctacttttgg aacaccgtgt
    11461 accagaggtt gtgaaaaagt cgcttagttt cgagtagtaa gaaaaatttt gtgaaaatgg
    11521 ctttctacat ttttgcataa atttggctta ataccgagaa actggctttt gaacaccgtt
    11581 tattcagata agggactgtg acaagacttt tgtcacagtt tctttttgtt tattcttttt
    11641 tatctttgta tggcaataat agacaatata acgaaaatta tttaatggct tttggaagac
    11701 catggaaaac cttgtacaaa gctatttttt ctgaaaaatt ccttgaaacc tcacgaaaaa
    11761 atcattattt tatgaaaaag acaaaaaaaa gtgttgattt tttaataatt attcattaaa
    11821 ctcttaaaca acttcagaaa atattaatta ttctgaaaat tccaataaag gggtacgtaa
    11881 aatgaaaaag aaatttacat ttggtttggt tgcggtatgc ggccttgtac ttgctggatg
    11941 ttatggcggc agttcagcca caagcagtag ttcagcaaaa agcggttcag cagatggagg
    12001 aggcgtattt aacttagtcg tgcctcaaga aatgcctaca gctgacttat cagttgcaac
    12061 agatacaatc agttttacag cattgaataa cgtttatgaa ggaatttacc gtcttgatga
    12121 agacagcaaa ccacagccgg caggagctag cgagttggca gaagtcagtg aagacggatt
    12181 aacttacaaa atcaaattac gagaagacgc aaaatggtca aatggtgatc ctgttacagc
    12241 agcggattac gtttatggct ggcaacgaac agtagaccca gctacagctt ctgaatatgc
    12301 ttacctgtat gcgccagttg aaaatgcaga agacattaca gctggaaaga aagataaatc
    12361 agaactaggg atcaaagcag ttggtgacta tgaattagaa atcaaattga cgaaacaaac
    12421 accatatttc caatatctgc ttgctttccc atcatttttc ccacaaaaac aatccgtagt
    12481 ggaagaaaat ggggatgcct atgcatctgc aagtgataaa gctgtttata atggaccatt
    12541 cacattagaa gattttgatg gaccaggaac agatacagaa tggacttata agaaaaatga
    12601 tcattactgg gacaaagata cagtaaaact ttctgaaatc aaagtcagtg tagtaaaaga
    12661 atcttctact gcattgaact tattcaaaga tggccaagca gatgacgtga tcttgtctgg
    12721 tgagttagca cagcaaaacg caaacgatcc agcttatact tctgttaaag aagcgcggac
    12781 aagctatatc gaattcaatc aacgtgaaaa gaattcacca ttcaacaatg tgaacctaag
    12841 aaaagctatt tcttattcta tcgatcgtga agctttagta aaacaagtac taggtgacgg
    12901 atcagttgtc tctacaggat tgatcccatc agatatggta aaaagtccaa caacgaacga
    12961 agactttgca aaagaagctg gaaaattagt ttcttatgac aaagataaag caaaagaata
    13021 ttgggaaaaa gcgaaaaaag aattgggcgt tgattcttta gaatttgatt tgatggcttc
    13081 tgatgatgat tcatcgaaaa aagtaatcga atatgttcaa aactcaatcc aagaaaactt
    13141 ggatggcgta acagtcaaac caacacctgt tccattctct gtacgtcttg accgctcaac
    13201 ttcgggtgat tttgacgcag tactaggcgg atgggctgct gactatgcag acccaagcag
    13261 ctttactgat ttgtttgtca caggtaattc atacaaccgt ggtcgctgga gcaatgcaga
    13321 atatgacgaa gcagttaaag cagcttcatc aacagacgct ggcgatgaac aagcccgttg
    13381 ggatgatttc caaaaagcaa tcaagatcat ctctgatgat atgggtgtcg ttccggtgta
    13441 ccaaaaagca gaaggtcacc tagtgaatga aaaaatcaaa ggtatcgttc accatgcggc
    13501 aggcgcttca tgggattaca aatggacata cgttgaagaa taatcgttaa taccgtgagc
    13561 atgagaaaaa acgaggctgc ggcaaatgtc gcaacctcgt tctcatattc acttatccta
    13621 acaaagaaaa aagcatgaaa atagcagaaa gagcaattag tattccggca atgatcagcc
    13681 aaaagctgta cataccgtag ctggcagacg agagcgagga aaattctggt tttttctttc
    13741 ggttccttgt tcgtgcaaga tggaccgatc gctgaaaatg atcgaagaaa cccgaagaaa
    13801 agacccataa aaaaccgccg ataatcagaa acggcaatga ccataagaat agatcattag
    13861 ataattgaat gagtgtaaag gaatggttga tgagattttt tattaaaata accagaatca
    13921 gtagaaaagc tactgcgtag gggatagatt tttttttcat ttgattgctc ctttattttt
    13981 ggttcattat ctaataatgt agcgaaacat ctgtcagaag tcaaaagcta gggggaaaca
    14041 ttagtgaata gttatatcaa atatgtatta aaacgtgtct ttttcatgat catcacgctt
    14101 tggttaattg caacaatcac ctttttcttg atgcaattgc tgccgggcac gccatatacg
    14161 aatcaggaac gtttgagtcc tgaaacaatc gagatgctaa ataaacaagt cggtttggat
    14221 aaaccagtta tcgtacaata tgggatttat ctttctaatt tgcttcaagg agattttgga
    14281 atttccttcc aatttaaaaa ccagccagtc gcacatttgt tagctggaag aatcggacca
    14341 tccttgcagt tagggttaca ggcaattatc tttggaacag tcctagggac gattttagga
    14401 acgatctctg ccatgaagca aaatacatgg gcagatacat cttctacact cgtagcgata
    14461 ttaggtcgtt ccattccaaa ctttgtattt gctgttttat tacaatatat ctttgcaatc
    14521 aaattacaag ttttgccaat cgcaaaatgg gatggctttg tgtacacaat cttaccaaca
    14581 attgcgttgg ccatgtcgcc gcttgctgac tctgctcggt ttatccgtac ggaaatggtg
    14641 gaagtactgc atagtgatta tgtggaatta gcaaaagcaa aaggattgag ccgttgggaa
    14701 atcgccttta aacatggtct tcgaaatagt ttgattccat tgatgacttt attaggaccg
    14761 ttagctgtag cattgatgac cggatcactg gtggtagaaa atatctttgc tattcctggt
    14821 atcggggaac aattcgttaa gtcaatcaca acaaatgatt atcctacgat catggctgta
    14881 acgatccttt actcatttat gctgattttt gttattttga tcgtggatct attgtacggt
    14941 ctagttgatc caagaattcg tgtttcagaa gggagccgag gataaatgga aatgatacca
    15001 aagcgttatg aaacagttgc agatatccct gcggatgaat ttcttccatt acaaaaaaat
    15061 accgaagaag aacgtgaaca gattactgca ccttctttga attttatcca agattcatgg
    15121 cgccgtttaa agaaaaacaa agctgccgtt atttcaatgg ttttactatt ggtcatcatt
    15181 ttgatctctg tgatcacaat ctttgtttct cctcatgatc caactgccca aaacgtggct
    15241 tatatcaatt tgcctcctcg tattccggga atcaatatcg atggattgaa tggaaaagca
    15301 atggttggtg gtgaacttgt tgataaatat gctcaagcaa acgtcccttc aaacgtaaat
    15361 ttctttttag gaacagacgg tttgggacgt gatgtattaa gccgcttatt tatgggaatg
    15421 agaatctcgt tgctgatcgc atttattgct gcattactag atgtaacgat tggtgtagct
    15481 tacgggttgg tttctggttt gttaggcgga cgtgtcgata acgtgatgca acgttttttg
    15541 gaaatcttat caggaattcc aaacctcgtt gtcatgattt tgatgttggt cgtttttgaa
    15601 ccaggaattt tttcaatcgt ggctgctatg gccatcacaa actggatacc aatggcacgt
    15661 atcgttcgtg cacaaacaat gaaattaaaa gatcaagaat atgttttagc tggattgaca
    15721 ttaggtgaat caagaatgaa aattgcattc aaacatattt tgccgaatat ctctagtgtc
    15781 attattattc aaatgatgtt tagtattcca tctgctattt tcttcgaagc ctttttaagc
    15841 tttatcggtt taggtcttac gcctccgtca gcttcattag gaacgatgtt gagcgaaggg
    15901 tacaagacat tcctgtatct gccttatcta ttatggattc cggcagtaac tttatcggtt
    15961 atcatgattg gctttaactt acttgctgat ggattgcgtg atgcctttga tcctaaaatg
    16021 aaagagtgaa gacgatgacg aaaattttag aagtgaaaga cttagaaatc tcttttgata
    16081 cgtatgctgg aaaggtacga gcgattcgcg gtgtcaattt tcatttgaat aaaggagaaa
    16141 cattggcgat cgttggggaa tctggaagtg gaaaatcagt gacgaccaga agcatcatgc
    16201 gcttgctgtc aagcaacgcc aatatagatg ctggtcaaat cctctttaaa ggtaacgata
    16261 tcgttggaaa atcagaaaga gagatgcaaa aaatccgtgg taaagaaata gcgatgattt
    16321 tccaagatcc aatgacctct ttagatccga cgatgcccat tgggaagcaa gtcgctgagt
    16381 cccttagaaa gcataataaa gtttcaaaaa aagagggaca aaaagctgca ttagaattgc
    16441 ttaaattagt tggaataccc gaagctgaga aacgtatcaa cagttatccg catcagttct
    16501 caggaggtca gcgtcaacgg atcgtgatcg cgattgcact ggtatgttat ccggaaatcc
    16561 tgattgctga tgaacctaca acagcgcttg atgtgactat tcaagcacag attttggagc
    16621 tgctgaaaga aattcagaca aaaatcgata catcaattat ctttatcact catgaccttg
    16681 gtgtagtagc gaatgtcgct gatcgtgtcg ctgtcatgta tggaggaaga atcgttgaag
    16741 ttggaacatc ggaagaaatt ttctacaacc cgcaacatcc ctatacttgg ggactgcttg
    16801 gttcaatgcc gacattagat agtgcgaacg atcgattgta tgctattcct ggttctccac
    16861 cggatttatt gaacccgcca aaaggagacg cattctatcc aagaaatgaa ttcgcgatga
    16921 aaatcgatgc agaacaagaa ccaccatttt ttgaactatc aaaaacacac aaggcagcaa
    16981 cttggctact tgctccgcag gcacccaaag tgacaccgcc agaagaaatc caacgtcgct
    17041 gggctatctt taaagagaaa caaaaacata cttacggtca aggaggaatt gctgatgcca
    17101 aataaactac ttgaagttaa aggattgaaa cagtatttca atgtaggaaa aaagaatgaa
    17161 gtacatgctg taaatgacat cagctttcac atttatgaag gagaaacatt cggtttagtt
    17221 ggagaatctg gaagcggaaa atccactact ggccgtacga tcattcgctt gaatgaacct
    17281 actgatggtg agatattatt cgacggtcaa gatataacga agatcaaaga taaaaaaggt
    17341 ttgacgaaat tccggcatga tgttcagatg atctttcaag atccatacgc ttcgttgaat
    17401 cctcgtatga aagtcagaga tatcattgcc gaaggaattg atgttaatgg actagctaaa
    17461 tcatctgaag agcgtgcaaa aaaagtcgat gatctattga gaactgttgg attgaatcca
    17521 agccacggta ctcgttatcc tcatgaattt tcaggtgggc aacgccagcg tattggtatt
    17581 gcacgagcat tggcagtaaa accgcgtttt atcatttgtg atgaaccaat ttctgcacta
    17641 gatgtgtcga tccaagccca agtggtcaat ttgttgcaag atctccaaaa agaacatcaa
    17701 ttgacctatc tatttattgc acatgatttg tcgatggtta aacatattag tgaccgaatc
    17761 ggtgtaatgc ataacggctt gttattagag atgggaacaa gtgatgaaat ctataatcac
    17821 ggtgttcatc cttatacaga aagtttgtta tctgctattc ctctaccaga tccagaccat
    17881 gagagaaaac gcagaagaat caaatatcaa cctgaaccag atgacggaca agtacgtcaa
    17941 cttcgtgaaa tcgcaccaga acattttgtc tatgcaactg agcaggaagt cccatattac
    18001 gcaaaaaaac taaaacgaca aaaggaatct cttctggttg ctaactagga gggacataga
    18061 tgctaaaagc ttttaaattc cgtatgtatc cgacagaaga acaaaaacag caattgattc
    18121 gtacttttgg atgtgctcga tttacctata accatttgct gaaaaagcgg cagaaaagtt
    18181 ggcaacaaac aggagtagcc aatttttcgc tcacacccgc tacattaaaa aaagaatacc
    18241 cattcttaaa agaagtagac agtcttgctt tagcaaatgc acagcttaat ttggatcggg
    18301 cttttcgcaa ttatttcaag ggacgtgcca gcttcccaaa gcttaaaacg aaaaaaagta
    18361 tgtggcaatc ttatacgacc aacaaccaaa cccgtacgat ctatctggaa aatggctact
    18421 taaagttacc aaaacaaaaa gaactaatca agataaatca gcatcggcca gttgaaggat
    18481 cgattcgctc cgctacgatt tctgctcgat ataatgaaga attttatgtc gcactacttt
    18541 gtgatgtttc gccagtaaaa aaagaatctt tagctaaatg gatcgggatc gcctatcatc
    18601 cgaagacatt gatccaaaca tctcgacctc tagaagtaac tttaccaaaa tttcaccaga
    18661 cagaagaaaa gctgcaacat gcacagcgga aattgaatgt gaaagtccgc tccgctcacc
    18721 atagaaagat caggttagat caggcaagca attatcagaa acaaaagaga aaagtaatgg
    18781 atctttatct gaaacaaaag aaccagcgag aagattatct tgaacaatta tctgggaaat
    18841 tggtcaaaca atatgactac ttatttgtag aatcttttcc aaaagaagag gcacatgctg
    18901 atttttctat tcacgactgg cataaattaa taacaaaatt gcgttataag tcccaatggt
    18961 ataataaaaa atttcttttg atcaataccg acggagcaga agaaagcaat tccgtaagaa
    19021 agagccaagt agtagaaaaa atgggcagac attcggttat caagggataa aaaatgaatg
    19081 aaactgatag gagcgttagg gttagccttg ttaataaaaa gtcttaatga cttttgtttc
    19141 taggaatttt tcttttgaag aaaagctcac taattatgac aaaccaataa aacaatagca
    19201 gaaaaagttc gtacgagctt tttctgctat tgttaatttt ttattcagaa agtttacgaa
    19261 tacatcaaaa ttctgaaaca aaaaacgtaa ctcacaaaga gttgtttttg cgttttttct
    19321 tgaatgatta ggtctgaatc ttttgctcac aaccttattc atggtgcccc ggcttgagct
    19381 gatagatttt tgaaggacgg ccgattccta caggacgttc accgatctca tcgaaatatt
    19441 ggagcatagc tttcttaaaa ttggaatgat cgatcgtttt ataatcgatc cctaagaatt
    19501 tagcaaatac ttttcgagct tccgtaatcg tgaaatcttt acctaaaact tgtagaactt
    19561 gcggctcgtg ttccatttta ttgactacac ggttgaatgc tttcaagatg atttgactat
    19621 ggtcaaaagc caaagtatct ttgccagaag actctccagt gactaaatca agcgagatag
    19681 ccacttctcc gctagataaa tggatcttgt tactatgacg ttctagtgtg aaccagcgta
    19741 cttccttggc atcatctccg gcgattagcg gttcttctcc aataaaggct aagtagctga
    19801 ctgtgacgac ccagcctcgc ggatctcgat taggcgtact gaatgtatgc agttgctcaa
    19861 tattttcttt tgaaataacc acaccagttt cttcctttgt ttctcttaag acgctttctc
    19921 cagttgactc atccttttgg acaaagccac caggcaatgc ccatgaattt cgataaggat
    19981 gcccttttct ttggatcaac aaaactttca gctgatcttc ttctttgtta tagcacatca
    20041 gtacgatatc gacagtcaaa gagggcttgt catattctgg acgttcctgt ttttgatacc
    20101 agttaagaaa ttcttcttca cttgctttat gttcataata taatttttct tcttctttag
    20161 aagcaaaacg gttcatctga ccacctccat tgacagtaaa agtacttttt accttattgt
    20221 agcagactct gaaaaaaaac caaggattta cttgaaaaat ctaggttatc atttaaatat
    20281 gaaaaataaa tggaagtatg agattgaata aaaagaaaag cagataaaac ggcaatactt
    20341 tataccgctc ttcacgaatg gcaaagaagc cacaaataac gatatatacg tcaatgataa
    20401 aacgcaaaat ccaagctgct gtcataaaac tgttcaaatg cccatggagg ttaaaataac
    20461 catacatacg aatataaaaa tagctgaata ttaaatgtgc taatgaaaga aaagcaatac
    20521 taagtgtaaa aaaaagatgt ttcttctgca tgattgttcc agtcccttac taagatagat
    20581 agtctttatt ataccacgtg tgaggggaga aataaaaagc cgttttttag tagatatgaa
    20641 acaaaacaaa aacccgcatt tattcaacct attcatcctt ttaaaggata gattgaacgt
    20701 agatagaagt cgtttatcca gtctttttca ttttgaaaaa aagctaacag catgcttttt
    20761 ttatggtata ctagcaagga aacgaattcg catcatgcga taaaggagga agagccccgt
    20821 ggcagaaaag gaaacttttt atatcactac gccaatctat tatccaagcg gcaaacttca
    20881 tatcggcaac tcgtatacta cgattgcatg tgacgcaatt gctcgttata aacggttgat
    20941 gggctttgat gtgttttatc tcactggagt ggatgaacat ggtcaaaaaa tcgaaacaaa
    21001 agcagaagaa ctaggtgtaa aaccgcaaga atatgtagat aaaatggcgg ctgatgtcaa
    21061 gaaattatgg aaaacgcttg atatcagtta tgataaattc attcgcacga cagatgatta
    21121 ccataaagca gctgttcaaa aaatatttga tcgtttatta gaacagggag atatttactt
    21181 aggtgaatat gaaggctggt actctgtttc tgacgaagaa ttctttacag aaacacaact
    21241 tgcagaagtg tacaaagatg acgaaggtaa cgtgatcggt ggtaaagcgc caagtggaca
    21301 cgaagtagaa ttggtaaaag aagaatcata cttcttccgc atgagcaaat acgcagaccg
    21361 tctattggaa tactatgaag aacatcccga attcatccag ccagaatctc gtaaaaacga
    21421 aatgatcaac aactttatca aaccaggatt agaggatctt gcagtttcac gaacaacttt
    21481 tacttgggga gtaccagtca agagtgatcc gaaacatgtg gtgtatgtat ggatcgatgc
    21541 attatccaat tatattactg cactaggata tggctccgaa gatgaaagtc tattcgaaaa
    21601 atactggcct gcagatgttc agatggtcgg taaagaaatc gttcgctttc atacgatcta
    21661 ttggccaatc atgctgatgg cccttgattt gcctctacct aaaaaagtat ttgggcatgg
    21721 ctggctattg atgaaagacg gaaaaatgtc taaatcaaaa ggaaatgtcg tttatcctga
    21781 aatgcttgtg gaacgttatg gtttagatgc actccgttat tatttgcttc gtgctgttcc
    21841 gtttgggtca gatggtgtct ttacgccaga agattttgtt tcccgtgtca attatgatct
    21901 ggcaaatgat ctagggaact tattgaatcg aacgattgct atgatcaaca aatattgtga
    21961 tggactcgtt cctgactatg cctcacttgt cactccattt gatagtgaac tgtctacgac
    22021 ggctgctaac gttgtcggac gttaccatga tgcaatggaa aaaatggaat tcaatacagc
    22081 attggctgaa atctgggtgc tgatttctcg tgcaaacaaa tacattgatg aaacagaacc
    22141 atgggtatta gcaaaagacg aagaaaagaa agctgaattg aatagtgtca tgatccattt
    22201 agctgaatct ttgcgtatcg cagcgatatt acttcagcca atcatgacag aaacgccaga
    22261 gaagatcttc aatcaactag gattggactc tgaaacaatg aatcttgaag gattacactt
    22321 tggcgaattt ccttcaggaa caaaagtagt tgcaaaaggt acaccaatct tcccacgttt
    22381 agatatggaa gaagaagttg cttttattca agaaaaaatg tctgaaggta ctcaaacaaa
    22441 cgaagataca gtcaaatggg atccagaaga aacagaactt gtttcaacga aagaaaaaca
    22501 aatcaaattt gatgtatttg aaaaagtaga actaaaagta gcagaagtca tcaactgtca
    22561 aaaagtagaa ggggcagata agttgctgca attccgacta gatgctggag acagccaaga
    22621 ccgtcaaatc ttgtcaggaa tcgctgaatt ttatcctgat ccaagcgaat tgatcgggaa
    22681 aaaagtcgtc attgtagcaa atctgaaacc acgaaagatg cgtggacaaa tcagtcaagg
    22741 aatgatcctt tctgcagaag caccagatgg ctctttacaa gtaatcgaag caccaaaatc
    22801 catgccaaat ggctcagaaa tcgcatagaa attcaaaggg ggtatgacac tagttgtgtc
    22861 ataccccctt tttccgaaat ttgagaagca attttcgtaa attccttctt attacttgaa
    22921 gccaatcata taatacagaa acagagaact atccaaaata aatcctagaa aaagaagtac
    22981 gagcttcgct gatttttgtt aatttagaat aatactaata ggaatcgtaa aaacagaaaa
    23041 aaagcataat gataaagggt cgggcagatt ttaattgatt catctggtac tcttttttat
    23101 tgtccttaca ttcataaagt ttgaatgcaa tgactttatt acatctatat ttatatctaa
    23161 cagtagcgtt gatgtgttaa ttataagttc tatcacttta tgttcaatta gtttgtgctc
    23221 ttatatgtgt gattgaaaat atagataaaa aaacaatcat cggctttgac ttcgatgatt
    23281 gttttcttta ttttgtaaaa atcctatcac ttgattttat ggtaaccttt tatttttgtc
    23341 ttcaaacggc ttggtaagag agcagtaagc agtgcaaatg ggatcgaaaa gacataccag
    23401 aaaatcgtac gtccgatacc ataatgttga tagagctttg tcaacatcac gtgtaacaag
    23461 atcaacccta aaagcaagga gccagctata aaccaatgat tatcccaatg catgacttca
    23521 ctaagtgcca tcactccaaa aggatagaaa taaaaataga tcccgactaa ggtcatccgc
    23581 cagttctttg taaatgttcc gatattcaat aaaaagaaaa tcgctgaaag caaaataccg
    23641 ataggcggaa aaagcccaac caaaaaggaa tacaacatcc cccacaagat catcacagga
    23701 cctttgatca atgcaacgat tgcgatgaac aaagtcagca atattagttt ttgtgataac
    23761 gttgcaacag tcattaatcc aaataaaatt aatgtgaacc aatgccaatg ggactgctgt
    23821 tgttctttac gggaaaaatc gacccggttg gataaataat ctgctaattt cggataagaa
    23881 cgcttaaaat ccataaactg ttcctccttc gtggaaaagg taggtttagt atacgaaaaa
    23941 taaaaaagaa atacatccgt cttaagaggg agtttttcac tgaaagtgga gaaaaaagaa
    24001 tggtaaatta aaatattctc agttatactg aaaattatac caaatttcaa agaagaaaga
    24061 ggatgggaat gaaaggcaaa ctaaaacgag aaatcaatct gtttggcgca ttagctaccg
    24121 ttatgggaac cgtgatcggt gcaggcgtct tcttcaaaac tgctgctgtt actgctagta
    24181 ctcagtctac aagtttgaca ttgctagctt ggcttttggg tggtttctta acgatctgtg
    24241 ctggattgac agtggctgaa ttagctacag caatcccgga aactggtgga gctgtaaaat
    24301 atattgaagc tgcgtatgga aaattgccca gctttttatt aggatgggcg caaagcttga
    24361 tttactttcc tgctaatatt gctgcattat cgattatctt tgcgacacaa ttgacgaact
    24421 tattacaatt atctaccgat tatcttttgt tgattgcagt gatcaccgct gtttctgtga
    24481 cagggttgaa tttgttaggg acaaaagtcg gtgcttctgt acaatcagtg acgttgattg
    24541 ttaaattaat accaattgct gtgattgtca tatggggatt attgacccca ggacaaggaa
    24601 ctgtccagtt atttccaatc gaagctggaa aagatgttac ttttgtcgaa ggattgagca
    24661 gtgcgttgtt agcaacgttg tttgcttatg acggctggtt aggtgtcggt gcaatggcag
    24721 gggaaatgaa acggccggaa aaagatttgc ctaaagcaat tattcttggt ttgagttttg
    24781 taacagtcgt ttatctattg atcaattttg tttttttgaa aactttgccg attgatcatc
    24841 ttgccggtaa tttgaatgca gcttctgaag catcagacgt catttttgga ggaatcggtg
    24901 gaaagctggt cactattggg atcttgatct cggtatacgg tgcattgaac ggttatacac
    24961 taactgggat ccgcgtgcct tatgcgatgg ctttagaaga cgagctacct ttcagtaaac
    25021 agctgacaaa gctttcaaag aagtttacgg ttccttatgt tcctgcggtt ttccagttag
    25081 cagtagcttg tattatgatg agccttggtt ctttcgactt tttaacagac atgttgattt
    25141 ttgtgatgtg gctattcagt ttactgattt ttatcggtgt ctttatttta agaaaaaaag
    25201 cgccggaatt gccacgtcca tataaagtac cactttatcc aatcgttccg attattgcga
    25261 ttctcggtgc tattttcatt ttaggtatga cgatgatgac gcaaacgaaa ttagcattga
    25321 tcggtatagg tgttaccttg ataggtatcc cagtctatta tcaaaaaaag aaaaaacgct
    25381 cagaagagta aaatatttca acaacagaca gaaaaagtac gaactaggtc ctctagatct
    25441 tcgtgctttt tctatttcaa taaaacaagt tcttctattt tgatttttgc tacttctgca
    25501 agtagtgctt atgtgctaaa gtaagaatga aaaggagaga taaacatgat atttgattct
    25561 catacccatt taaatgctga acagttcaat gaagatattc cagaaacgat tcagcgagca
    25621 caagaaatgg gggtaacgaa aatggcggtc gtgggcttcg atacgccaac gattgaaaag
    25681 tctttgcaat taagccatga ctatccaaat atatacagta ttatcggctg gcatccgaca
    25741 gaggctggca gttatacgaa agaaatcgaa aataaattac aagaacaatt gactttacca
    25801 aaggttgtag cattagggga aattggcttg gattatcatt ggatggaaga cccgaaagaa
    25861 gtccaagata aagtatttcg cagacagatt gctatcgcaa aagaaatgaa cctaccaatc
    25921 agtattcata ctagagaagc acttgaagac acgtatcaaa tcctaaaaga agaagatgtt
    25981 cgagatatcg gtggaatcat gcatagtttc agtggtgatt atgaatgggc aaaacgattt
    26041 cttgacttag ggatgcatat ttcatttagc ggtgtcgtca ctttcaaaaa agcattagac
    26101 gtccaagagg ctgcagcaaa tgtaccaatc gaacgattac ttgtggaaac agatgcccct
    26161 tatctggcac cagttccttt tagaggaaag cgaaatgaac caggctatac tcgttataca
    26221 gtagaaaaga ttgctgaact tcggaatctt ccgacagaag aagtggctgc ccaaacatgg
    26281 acaaatgcgc accgattatt tggtctaagc gaacatggat aagatgaaga tccaagagat
    26341 tatagtcgtt gaaggaaaag acgatacaag aaggctgcgg gaagtagttg atgcagatac
    26401 gatagaaaca atagggtctg caatcaatga agagatcctg atgcaaattg aacatgccca
    26461 agagactcgt ggagtgatcg tttttaccga tcctgatttt tcaggagaaa aaataagaaa
    26521 aatcattatg gaagctgttc ctcaggcaaa acatgctttt ttgccgcgca cacaagctaa
    26581 accaaagaaa aagggaagcc ttggtgtcga gcatgcaagt gatgctgcta ttttagaagc
    26641 attgaaaaag gtcgtgacgc cagacatttc ccaaacggct gtttctgaaa tcactagaca
    26701 agacctttta gactatggtt taatagctgg ggcacaagca aaagaaaaac gagaaaaact
    26761 aggtgacgag ttgcgcatcg gttacacgaa cggcaaacaa ttggaaaaac gtttgaagat
    26821 gtttcgtatt acgccgaaag aactcgccca agcaatgaaa atagtggagg aacaacatga
    26881 gtgaatatcg tgaaattgcg acaccctcta gaacaaaaga gattttattg aaacatggtt
    26941 tttcatttaa aaaaagttta ggacaaaatt ttctgactga accaaatatt ttaaggaaaa
    27001 tcgtagaaac tgcgcaaata gatgaacata cgaacgtgat cgaagttgga ccaggaatcg
    27061 gtgcattgac ggaacaatta gccaagcatg caaaacaagt cgtagcattt gagatcgatg
    27121 atcgtttgat ccctgtattg gctgatacga tgcaacccta tgacaatgtg actattgtac
    27181 atcaagatat tttaaagacc gatttgtcaa cagctgttag agaaacattt catgaggagc
    27241 ttccgttaaa agtcgtagcg aatttacctt actatattac gacaccgatc atgatgcatt
    27301 ttcttgaatc agatttggtt gtggatgaac tagtcgtcat gatgcaaaaa gaagtagctg
    27361 atcgtatctc tgctgaacca ggaacgaaag cttatggttc attatcgatt gctgttcaat
    27421 attatatgga agccagttta gcattcatcg taccaaaaac agtatttgtc cctcagccaa
    27481 atgtagattc agcgattttg aaactgacaa gaagagatat accggcagtc gaagtgacag
    27541 acgaaaaagc atttttccga ttgacaaaag cggcatttca gcaaagaaga aaaacgcttt
    27601 ggaataactt gcagcattcg tatggcaaag atgaccagac aaaagcatgg ttagcaaaaa
    27661 gcttggaaac agctggaatc gatccaaaac gtcgtgggga gactttgtca ttacaagaat
    27721 tcgccgcatt aagcaatgcg atgtcagaaa atatgcagta aaaaaagagg ttgtgacaga
    27781 agttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
    27841 atgtttttcg tttgtttggg cttgttaccg cagatgcaag cttttgaaca ccgtttaaga
    27901 aagaggttgt gggcctgccg tttaactcta agtcacaagg aacaatttca aaaaacagct
    27961 tctcatgttt ttgagttagt tggtcttatt acttgatgca agcttttgac cactattatg
    28021 ccgaacaagg agatgtgaca caaattttgt cacatctcct atttttgttt cttgatcaaa
    28081 aaattcttat acttgatact cacactcatt ttgtatggat ttagaaaaga atatgtgtct
    28141 tgacagttat tagtaagtga gttatactaa gtcgacattt atgtgtcaag acatattagg
    28201 agagagaaaa aatgagaaaa aattcagttc gaaaactaac gatttctgct ttattgattg
    28261 ggatgggggt cattatccca atggtcatgc ctaaaatcat gattccacca gcttccttta
    28321 ctttggcgtc tcatgtgcca ttatttattg cgatgttttt tacaccagga gttgcagtgg
    28381 ctgttgcgct aggtacgaca tttggtttct ttttaacaac gccggttatc atcgcattgc
    28441 gagcattgtc acatcttgtg tttgcgttga ttggggcttg gtacttgcaa aagcatccga
    28501 gtatcgtttt aaaaaatgga cagtttactt ttgctaattg gcgtttccaa gggttcaatt
    28561 ttgtgatcgg tgtgattcat tctttagcag aaatgctagt agtcagcatc ttttacttta
    28621 ttggtaatat gcctgaaaca tattattctc aaggatattt ttatacagta ttcatcttga
    28681 tggggattgg tgggcttatc cacagtttag ttgattataa tattgcttat tttatagcag
    28741 gaacactcag caaatatttt gatatccccg tatttacggc agcaaaaaaa gaaaatcgga
    28801 aagagtcgat tcaagggtac gcgaagaaaa cgacatatta gacatgatct ctcaaaaaaa
    28861 aaactctcat ctagtaatgg ataataaatc ctctaagatg agagtttttt gtcctaaaaa
    28921 gagctgcacc aacatcttct ggcacagctc taacaggggg caatttatgg aagtttgatg
    28981 acttgttctg tttctttatc aaagaagtgg cctttgctga tgttgaaggc aaggtcgact
    29041 atttcgcctg gtttatggaa atctcttgca tctacttttg agatgaactc agtagaacca
    29101 gtttttgtgt aaagcatcgt ttcagctcca agtaattcgg aaacgacaac ttctgatttc
    29161 actacggctt caggtgaagc ttcaagggct acttgttcac tgtggatgtc ttctggacga
    29221 ataccgaaga taacttcttt tccttcataa cctttttcaa ctaatacttt gttttttcct
    29281 tcaggaatac gaagcttaag cccatgtcca tctgtgataa caccattttt caatgtcaca
    29341 ttaaagaagt tcatggcagg agatccaata aagccggcaa cgaacatatt gacgggtgta
    29401 tcataaactt ctttcggtgt accgatttgt tggataaatc cgtctttcat gataacgatt
    29461 cggtcagcca tagtcattgc ttctgtctga tcgtgagtaa catagatggt cgttgtttca
    29521 agacgctggt gaagtttggc aatctcagca cgcatggcta cacgtaattt agcatctaaa
    29581 tttgataatg gctcatccat taagaagact tttgcatccc gaacgattgc ccgtcccaat
    29641 gccacacgtt ggcgttgtcc accagataaa gcagctggtt ttcgtttcaa gtattctgtc
    29701 agacctaaga tctctgctgc attgtccaca cgttttttga tgtctgcttt atcatattta
    29761 cgtaatttca agccaaaagc catattatca aaaacagtca tatgaggata caatgcgtag
    29821 ttttggaaaa ccatggcaat atcacggtct ttaggtgcca cgtcattcat gacttttccg
    29881 ccgatattta gttctccttc agagatatct tccagtcccg cgatcattcg caaagtagtt
    29941 gactttccac aaccggaagg accaacaaaa acgataaatt cacggtcttt gattgataga
    30001 ttaaaatcag ttacagaata attttctgca ttgtcatatt ttttatatac gtttttcaga
    30061 gccatttcta ccataatacg ttccacactt tcttttttag ttttattaac tatttaaagt
    30121 ataaatgaaa gcgttaacta acttcaatgt catgttgaac aagaattttt tctttttttt
    30181 gtcaatgtgc tagttttaag ctttaataaa tatccataag aaataaaaac tgatacactg
    30241 gagtaaataa gtatgttaac aatatcaaaa ggagtgtctc ttatgaaaat cgctttgatc
    30301 gctcatgacc gaaaaaaacc tatgatggtg aaattagcaa cagcgtataa aacaatattg
    30361 gaaaaacatg aactttttgc taccgggacg actgggcaga ggatcacgga agcaaccggc
    30421 ttgccgatcc atcggtttaa atccggaccg ttaggcgggg atcagcaaat tggagccatg
    30481 atttcggagg atcagttaga tttggtgatt tttcttcgag acccgctggc tgcacagccc
    30541 catgaaccag atgtaacagc acttatccgt ttatctgatg tctatgagat tccgttagct
    30601 acgaatatcg gaacagctga ggtgctacta agaggactgg aagccggctt cctagatttt
    30661 cgaagaatcg tccatgaaat agacaagcat ccactttcat tttgatggat aagcgtatgg
    30721 aaaaataacg tttaatgttc gcctatatgt agctataaaa gctaaacaaa ctgataatat
    30781 gcgatataat acgaacgtat tggataatat aaacaataat attagttttt cgtatttgca
    30841 gtaaatgtga aaatatgcta tagtttctgt gggaaaagat cccaaaaata atacagagta
    30901 gaaaataaag cgttaagtgc taagaggatg ggatgttgcc tcttggacga aggacgtaat
    30961 gttcgcggtt ttattttcgc attcgctgca tgatacgatg tagcagctct taaaggagat
    31021 gctaaaaatg aagaaacacc ggttagacgc acgtatgatt gctattatgg gcttattgat
    31081 tgcattgatg gtcactttgt cccgactggt agcaatcgag accccattta ttaaaatcag
    31141 tgtcacattt attccacaag tgatcatggg cattcttttt ggtccttttt ggtcagggat
    31201 aggcgcagtt ttagctgatt tagtaggaat ggccctcttc tcgaaatctg cattctttat
    31261 tggatttaca ttgaatgcat ttattgaagg agcaatctac ggattctttt tctatcgaaa
    31321 agagatcact tggaaaaatg ccattctggc tacgcttagt gtcacgctga tcatcaactt
    31381 atttttgaca ccattatggc ttgcgttgat gtaccatgtg ccattgttca gctgggtcgt
    31441 ttgggcgccc cgcctgttaa aaacagtaat atggttaccg attcaatcaa ttgccattta
    31501 ttacgtagga cgtagtattc cttacaaaaa aattttaaga agtttagcga tccacgctaa
    31561 ataaaaaaca agcagtcttc ccatcccaga agactgcttg tttttattgt gctgtgaatt
    31621 gtgcttgata gagccgtcgg taataaccat ctttttgaag cagctcatca tgggtgccca
    31681 tttcgacaat agctccctga tccatcacta atatctggtc cgcatttttg attgtggaca
    31741 ggcgatgggc aatcacaaaa ctggtttttc cttccatcat tgctaagaag gcttgttgga
    31801 tttttttctc tgtcaatgta tcgactgagc tggtagcttc atctaagatc aacatagggg
    31861 gattactgat catcgttcga gcaatcgtca acagttggcg ttgtccatct gatatcttta
    31921 acccttgacc gccaatctta gtatctaatt tttccggcaa acgttctaca aattcataca
    31981 tataagaggc ttttagggca tcattgattt ctgcgtcact tgcttccgga ttcccaaaac
    32041 gaagattttc tcgcaatgta ctgtcaaata gccaagtatc ttgtaacacc attccaaaac
    32101 ttttacgtaa tgtatcacgt tggatagagc ggatgtcatg gtcatcgact gtaattgttc
    32161 cttttgttgt gtcataaaaa cgcatcagca aattaacaag tgtggatttt ccggcccctg
    32221 tttttcctac tatagcaatg gtttgacctg gataagcagt aaaattgaaa tcttcgatca
    32281 aacgctgctt ggggtcataa gaaaaagaaa catgatcaaa agagactttt cctttgatat
    32341 ttttgggttc caaggcatag gcatctggca aatcaggctt ttcttctggc tgatcgatca
    32401 aatcaaatgc tcgttctaga ccagaaaaag cggtttgcag ttgtgtcgtg attccagaaa
    32461 gttcgataaa tggtttagaa aactgacttg agtagatcgt aaagctggaa atcacaccaa
    32521 cagtgattga cgaaccactc ttcaatgcca ataggccgcc aactaaacca atcgataaat
    32581 aagcaaggtg atctacaaaa cgagaagacg ggtttgtcaa ggaagaagaa aattgtgccc
    32641 gttgtccttt ttcatataga tcagcattta gcagttcaaa ttgctgttga ctagcttctt
    32701 cttgctgaaa agctttcacg attttctggt taccgattcg ttcggtcaca aagcccgaga
    32761 tatccccaat aatcctttgc tgtctatcga aatttgtttg tgaagcacga gcaacgatcc
    32821 aactcacaag gaaaataatt ggtgtagaac aaagaacgac caatgtcaga agtggactta
    32881 gacgcatcat aaaaataaac gaaagaatga tgattgccac tccagaaaat aattgattga
    32941 aggccgcaga aattgcaatg gaaatattgt ccatatcatt tgtaaatcga ctgacgatat
    33001 ttccgtggga gttttgatca taataacgaa gtggcaatcg attcagatga gcaaatgcat
    33061 cttttcttaa ttttgcaacg gataagtaag ctaaccgatt gcttaacact tggatgagcc
    33121 attgactgac tacgttgatt aaaacgatac caccgaatag aataagtaaa tggaacagtc
    33181 gattgaagcg gacctgtcct tctccaatca tttgatcgat actttgacca atttgtaacg
    33241 tcatcacaac tgttgcaatc ccattgatga ttcctagtat cacagctagc caaatgtctt
    33301 ttgcataggc agtaaggtaa ggaatgaatc ggcggagcgt ctgcaaggat aattttttta
    33361 ctttcataac tattcttcct cctgtgatgc gacgatttcc tgatattctg ctgattgagc
    33421 aagtaattct tcatgggtac cctgagcagc aatcttacct tgatccatca ctaagatatg
    33481 atgtgcttct tgaatagagc ggacacgttg ggagataata atcactgtac tgccaagata
    33541 agtctgtagc gccttacgca aatccaagtc tgtttgataa tccaacgcac ttaaagagtc
    33601 atctaaaatc agtaatgctg gcttggcaat caaagctctt gcaatcgtca gtcgctgttt
    33661 ttgtccgcca gagaaatttt tcccattttc catgactttt gtatctaaac catttggaag
    33721 ctgacggaca aattcttctg cctgagcgat tttcagagct tcccaacatt ctttttctgt
    33781 ggcgtctttc ttaccccatt gaaggttttc acgaatagtc ccggtaaata gtacagaagt
    33841 ttggggaaca agagcaattt gctgacggag atgatccagc tgccaagaac gaacatccat
    33901 tccgtccatt ataactgtac cggaactgac atcatagaaa cgcggaatca attgtgtcaa
    33961 agtcgttttc ccgcttccgg ttggaccaat gattccaagg actgtatttc gcttcagctg
    34021 aaatgtaata ttttggagag aaagaccaga atcaggagta taacggaagt caacatggtc
    34081 aaaagcgata attgcttcag aagaaaaatc gggagtagta ctgacctcag cttcaagaat
    34141 cgaattttct gtgtctagca cctctttcac ccgatttcct gaagcttcgg cacgtgtgaa
    34201 gatcacgacg aggtttgaaa caacgatcag tgccaaaagc atttgattca tataattgat
    34261 caaagcaaga acttgtcctt gttgcaagct accaatattt actttgatcc cgccaactgt
    34321 cagcaaacag atgatcccga cattcatgat caaggttgta gcaggtgata atagggcaga
    34381 gatattggaa acacgtaaat aattgttcgc tagatcattt gtgcttttat cgaacttctc
    34441 tgtttctttt tttgtctgcg caaatgcgcg gatgacacga acaccgctaa gattctgact
    34501 aacgtgttca ttgagctgat ctagtttttc ttgcactttt tggtagagag gaacggtttt
    34561 tttgatgata aaaaataaaa tgattgagaa aataggcaat aatgctaaga aaaacagacc
    34621 tacttcccag tcgatcacaa aagccatgac cactgacccg atacttaaaa acggtgctct
    34681 gatcaataaa cgaatgacca ttgctaaagc tagctgaagc tgattgatgt cgtttgtcat
    34741 tcgggtgatc aaggtatctg ttccaaaatg attcaactcg ttgtgagaga aagtattgat
    34801 ttttttgatt agctgattac gtaactctgt accaaatcct tgtgaagcta ctgaagcata
    34861 gtattggcaa atgatagcac aaatcagacc aatcacagac atagcgacca tccataagcc
    34921 catttcaatg atttttcctc ggtcattcat ttttagtcca ttatcgatca aggaagccat
    34981 cattaaaggc aggacaagtt cgaagacagc ttcaagaaat ttgaagaacg ggcccaaaat
    35041 gatttgctta cgatagtctt ttgcataacg taataactga atcatatgat tcctcctatt
    35101 atttatttgt ccacaattga aaaaacaagt actaccacat tgtagcttag gaaatttaga
    35161 atgaaaagag aacaagcctt ttcataaatg aaagttttat tatgaagaag ctttagaaat
    35221 tgaatagact atttttttta aaaaaaagaa aatgagaaaa catgccagtc tcttcattca
    35281 taggaacgtc tattttactt aatttttcta taaatgaatt ctgcataaaa aatatatcaa
    35341 gtaaatgttt atagatgaaa ctctatttac ttctttttta atttgttctt gcaatctttt
    35401 ttgctacgta atttttgtaa aaaagtgggt tgagagaaat ctaattgatt ttcaaagaag
    35461 gagtatgaaa atgagaaaaa tatttattcg tttattgtgg gtactatctt agaggaataa
    35521 ataatagtgg agaaaactat ggtttggcgc agattctatc aggtttgatg gaacgaagac
    35581 aacctcccga catcaagtca aaagctactt gagattttga tctgaaaata aaaaaagaaa
    35641 acaaggtagg aacatcaatg tttctacctt gttttcgtaa tcaatgggaa aaaactaaga
    35701 gtggtgtacg taatcaataa aaaggatggt gacaacagta cctctttact ctctgaaagc
    35761 atcataactc gtttttcctc attatgccaa taaattgttg ttataccaca tataaggatt
    35821 tgtaatagct aaaaacagac aggaaagttg aaatctgtga atcttatttt gtatggaagg
    35881 atttatagaa cgcaaaaatc aagataaaga tgaataaaaa tcctgtttcg gctatatatt
    35941 gcgtatctac gccaataaaa cgagtttgtt ctgtcatagc catataaagc aaaggcaaaa
    36001 atacaactac agcgagataa gatccgatga tttctcgaaa agttttccag gaagtatgtt
    36061 tcctgttttt atatttatcc gatttcattg aatagtcata gcctccttat tctttcatat
    36121 gttgaaattc cagtataaaa actggaagga actgtacgtt ttttagaata aaaattataa
    36181 gggataagta ggctgctgtc tataataaga tatatttttc aattctttat taaagatttt
    36241 attgacaaaa tgaatccgtt ttctatgatg gaggaggggt gaataagttg ttgaagaagg
    36301 aaattttatg ttacttagag aaccagacag cctttttcga tttagaacat atgaacgagg
    36361 tctttacagc acaaaggtta gctgatcatt ttggggtaaa aagaaacaca gtcagtcatt
    36421 atttgaacca gttaaatgat gagcaactac tagtcaaaat caattctcga ccagtgattt
    36481 atttccacaa agaagctttt gaaaaacaat tttttaagtt acgcacgaat ttttattttt
    36541 ctatcgatga tctcaaagag gaacagcctt ttttcgcgca accaaaagac ttattctctt
    36601 ttatgatcgg atacgacgcc agtttgaaag aaagtatcga acaaatcaaa acagctcttt
    36661 actatccaga tggcggatta ccacttttga ttacaggcga aagtggcaca ggtaaaagtt
    36721 atttagttaa tttggtctat cagtattgtc tgcttcatga tctgctagaa gattctgcac
    36781 cattcatcac ttttaattgt gcacaatatg cagataatcc tgaacttttg actagcaatt
    36841 tgtttggaca tgttaaggga gcgtacactg gtgcagaaga aaataaaaaa ggggcttttg
    36901 aagcagcaga tggtgggatc ttgtttttag acgaggtaca tcgcttaaca ccagaaggtc
    36961 aggaaaaact gttcacctat cttgatcaag gtgtgattta tcgtatggga gaacctaatt
    37021 tgtcacgccg gatcaaaaca cgtctctttt ttgctacgac tgaagagatt actagtcatt
    37081 tcttaacaac tttcattcga agaataccta tccgaatcga actacctgca cttaatcagc
    37141 gtagtcgtaa tgaacggcta gaactgattt actctttttt tctcgaagaa caaagaaaaa
    37201 tggggagccc tttgtcagtc agtggaccag ctttgtcact tttaacaagt cagaaattat
    37261 ccggaaacat aggagagctg aaaaatatcg tcaaagtttc agcagcaaaa gcatatgctg
    37321 aacagcgtga acagcctgaa atccatgtca cgatccatca tttgcaaaaa gaactacttg
    37381 cgcagcctat tactaaaaat acagcacagc aaggaatcta tattacgcaa gaccagacct
    37441 tagagcaatt aatggagaag caacagcccg aacagcagcg aatcgttaaa agttttgagc
    37501 aaatcctaat tgatttcaag aaaaataact gtctgctata ttcttgcgaa ggaaaattaa
    37561 agcaagaagt gattcaatta tttgattttt tgctgtttga gaccagtaga caagaaaaac
    37621 acgagctgct ggtttattgg acacaatcta tccgagagac ctttcgacaa atggaaactg
    37681 cctatcaaat caaattcgat ggaaacagtg tctatgcctt gagttactat ctctatcagc
    37741 gaagcactat caaatggtta ccggaagaac atgatattat ccaactaatc aaagaattag
    37801 aacaacaagt ttctcagtct tatccatcta gttatcacta tgtccaacga attctggaat
    37861 taagtaagat caaattagat attgaagaca caagtatgga tcggatttta ctgacgatct
    37921 atctaaaaaa agcgaaatgg tcaaaagaaa aacgactgcc aaaagcgatg attgctgctc
    37981 acggttatgc aactgctagc agtatggcaa atgtagtaaa tcgtttatta caagaggatc
    38041 tatttgaatc atttgatatg ccactagatg tcacaccaca gcagattgct tcagaaatcc
    38101 ttgattattg cgaaaacagc gatgtttcaa atggattgat catattagta gacatgggat
    38161 ctctgaaaga aatccatcag ttttttaaaa aacagttatc cgtaccgctt ttgattttga
    38221 ataatgtgac gacaccttta gcaatcacag taggggaatg cctgcaaaaa aatgtatcat
    38281 tagaagagat agcagaagag actgttcgac agatccagcc agaatggcga ctgctctatc
    38341 cagaggaaaa caaacccaaa gcactcatta ctacttgctt cactggaatc ggtacggcgg
    38401 tccacttaag tgaactgcta gaaaaaagct tacctacggc ttgtcagttg aaaatcatcc
    38461 catatgaata tcagcaatta aaagataaga aaaacagtga cccattattt tccatatacg
    38521 aagtcgtagg aatgattggc accacagatc ctgatatcac caatatcgac tatctttcat
    38581 tagaagacct catctcgggg gaaaagatgt ctgtactagc tgaatggttg gacactacta
    38641 tgaatgcttc agagaaggaa atattcaatc agcgcatcat tcgtaatttt tcattggaaa
    38701 aagtactgga ttccgtgact attctagata cagagaaggt gatgggagaa atcgatcatt
    38761 ttatgagaga attagaggtg cagttgaatc gaagcatcag caatcccaaa aaattagcgt
    38821 tgtatgtcca tgtgagctgt ctcattgaac gattgatccg acaaatgcca atcgaaacct
    38881 atcaaggatt agagaaatgg aagcagtgtc aaaaagaagg gttatctgct attaaatctg
    38941 cgtttagtgt cattgaagag cattatagtg tcatgatccc caattctgag attgcctata
    39001 tctatgatat tttgagtgaa gatacagagt ttctctcgat agaagaagaa ttttgaacat
    39061 aagcaagttg ttgccactaa ttttggcatg caacttgctt ttttagtatg tgtaagtgaa
    39121 aagaggagat gaaaaagtgg atagagaaat tatcttagct tcccatggaa aatttgcttc
    39181 agggatatta gattcattag agctgatcta tgggaaaaat cattctatta ctgtattcga
    39241 ttgctatact gatgagaatt ttgatttagc ggaaacagta aggcagcttt ttatgaggta
    39301 caaagataaa gaaatcattg ttctgacgga tctttttggc ggaagtgtca acaacgaatt
    39361 cttacaatat atcaatcggg atcatgtcta tctcgttgcc ggattgaatt tgccactatt
    39421 gattgagcta gtatctcggt tggacgctga agtagagaca actgttttga tccagcaggt
    39481 gctaactgat tcaaaaaaaa tgatccagtt ttgtaacgag cggctcaatc agaaaataga
    39541 agaagacgaa ttttaggagg aagaaaaatg attgtattga caagagtcga ccatcggtta
    39601 ttacacggac aagtagcttt ttcatggaca caaacaatag gcgcagactg tattttgatt
    39661 gctaatgatg atgtacctac gaatgagata cgaaagacaa cgatcaaatt agccaagcca
    39721 caaggagtca aactagtgat caaatcaatt gatgactcga ttgccgcgtt gaaaagcggg
    39781 gtgacagata aatataagtt attcatcgta gtggaatcga tcgaggatgc atataagctg
    39841 gcagaagcgt atccagaaat caaaaccatc aacttaggag ggatcaaggc aaaacctggt
    39901 actcgcagca tcggaaaagc ggtaaatatt ttaccagaag aggaggaacg cttgaaacga
    39961 ttaattgaca aacaaataga agtagaaatc cgtcaagtac cagcagataa aaaagtggat
    40021 gtaagagaag ttttgtaaga aaggaagaac ctcaatgatt gtacaagcag tcttattagg
    40081 aattgttgca tttgtcgcac aatcagaata tgcactaggg acatcgttgc tttctcgtcc
    40141 gattgtgacg gggctgttta caggaatcgt gttaggtgat gtaaaaactg ggatcattat
    40201 gggcgcaact ttagagttag catttatcgg atcgttctca gtaggtgctt cgattccacc
    40261 tgatgttgtg accggtggga ttttagggac ggcttttgct attacagctg gagccggtac
    40321 agaaaccgcg cttttactag gtttgccgat tgctacgttg acattgatat tgaaaaatat
    40381 ttacttaggg cttttgattc caattatgaa ccataaagca gacaattatg cagaagaagg
    40441 aaattataaa ggagtcgagc atatgcactt gcttgccggt atcggtcttt ccttgatgct
    40501 tggtgttgtg gtaatggttt cttatatggt gggaagcaat acgatcggaa atctattaaa
    40561 tatgattcct gacttcgttc aaaaggggtt gtcagtagct acagggttga ttccagctct
    40621 tggttttgcg atgcttgctc gtttgctgat caacaaacaa gttgcaccat attttttctt
    40681 aggttttgct atagcttctt acttagagat tcctgtgacc ggagtagcta tttttggtgc
    40741 gattttagca gttgtggtag tcaatattat gaatgtccgt caactaccac ttcaaacaac
    40801 tgcaggggag gtagaagaag atgaagaatt ctgagatcaa aaacagcaaa ataacgaaaa
    40861 aagagctaaa tcacgtattt tggcgttctt ttcaaatgga gttttcttgg aattatgaac
    40921 gtcagatgaa tatggcttat gtttatgcaa tgattccgat tttgaaaaag ttatatcagt
    40981 cgaaagaaga aatggcagca gctttgaaac ggcatctaga gttttttaac acgaccccac
    41041 atatcgttac actgatttta ggaatcacgg ctgcaatgga ggaagaaaat aaggatgatc
    41101 ctgaatttga tgtgactgcg atcgatagta tcaaaacatc tttgatggga cctcttgcag
    41161 ggttaggaga ttctttcttt tggggaactt tgcgattgat tgccacaggt gtgggtacct
    41221 ctttagccat gcaaggaaat attttaggcc ctatcttgtt cattttgatt ttcaatattc
    41281 cgcacattct gtttcgctat ctagcaacag gttggggata tcgcttagga acgggttttt
    41341 tgaaaaagat ccaggcaaac ggtatgatgg aaagtttgac attaggcgcg tctattattg
    41401 gactgatggt cgttggagca atgactgcca cgatgatcac catcaatatt cctttaaaaa
    41461 tcggttcagg agaaaatgct gtgactgttc aaagtatttt tgatgatatc gttccaaata
    41521 tcttatgtct tggagcattt ggcatagtgt tttatctatt gaagaaagaa gtgaaaccat
    41581 taacgatctt gatcggacta gctttctttg gaatttttgg ttcgtggata gggatttttt
    41641 aaaaaaggag cagagaaaat gtttacgatg caagattata tctatgaaga aaaggaagta
    41701 ctaagtacga tcttgaaaaa gaatgatttt tcgacaagag aacatatgaa aaaaacagtg
    41761 aatttactga tcttagcaac gggttcatct tataacgctt gtttagcagc gaagccagca
    41821 ttggaatctt atggtgatct tactgtcgat attcaagaac ctttttattt tttcaattat
    41881 ggaaaacttt ctccttcaat cgatactgtg attgctgtgt cacaaagcgg gaaaagtgct
    41941 tctactgttg aagcagtaaa aatgatacaa aaacaagggt tacctgttgt tgctatcaca
    42001 aatgacgtgc aaagtccact tgctttagaa gctgcacaaa ttattgattt aggtgccggg
    42061 gtagaatcag taggcttcgt tacaaaaggc tattctgcga ctgttttaca gttactactc
    42121 ttagggatag gaattggaat aagtaaaaat aaaatcagca aaaaaataga acaagactac
    42181 atgcaacagt tgcgcaaaat aatcaaccat ttaccagcga ttattcaaaa aacggaagaa
    42241 ttttttgatg gatatcaaag tttatttcgt ttagcacagc gtttcgtcac cattggatat
    42301 ggtccaaact ggggaacagc taaagaagca gagacgaaat tgactgaaac aatccgcgtt
    42361 ccgtcacaag gatttgagtt agaagcttat atgcacggcc catacctgga ggcagatgct
    42421 tcgcatttgc tattctttat tgaaggggaa agtgtaaata aggaacgttc gcaaaagttg
    42481 caaagatata tgagtcggta cgttggagag actctcacca ttaccacaaa aaaagcaaga
    42541 aacgagaaaa cacttggatt agcaatagaa tgtgatgaat atctgtctgt attagcgctt
    42601 gtcgttccgt ttcaactatt cgcttataaa accgcagtag ctaaaggtat tgacttgaac
    42661 aaaaagattt ttgaagactt tgatacagtc ttgaaaagta aattataaga ggtggaaatc
    42721 atgctgaaat tcaatgaaga agaacaaatc aaagcaataa aaggcgcact agctttacgt
    42781 ccgcaagtag aagaaatcat cgataaactt tatgcggaaa aatttgacgc tgtctattat
    42841 ctaggaatcg gtggaacgta tgcttcctcg atgcaagctg tcacttatat gaatgggaaa
    42901 agcaacttgc ctgtctttgt acagcatgct gcagaatatt acactactgg aaacaaaaga
    42961 ttaacaaaag attctatcgt tattttgtcc tctgttaccg gtacaactca agaagtagtg
    43021 aaagcagtag aacaaatcaa gaaagtagga gcgacactga ttggatttat cgataaagcg
    43081 aacagtaagt tgtctcagct atgcgatttt gtggtcactt atccagctcc tggaacagaa
    43141 caaatcaaat tttttatggc agcagatcga ttgatgtatt tgaatggaga attcgaagct
    43201 tattcagaat actatgaaca gctcgaacgt tatttaccaa cagggttagt ggaagcagag
    43261 aaaaaagcag atgcatttgg cttatcattt gcagaaaaac atcgacgtga tagtatgcat
    43321 tattttattg gagcagggaa tcaatgggga gctgtttatt catatgccat gtgttactgg
    43381 gaagaacaaa gctggcttcc atcgaaatct atccatgctg ccgagtttct tcacgggacg
    43441 ttagagatcg tagaagaaac aaccccagtc acattatttt taggcgaaga tgaacagaga
    43501 gtgttatctg aacgtgtggc taaacttttg ccaaaaattt gcagcaatta tacattgata
    43561 gacacaaaag actatccagt agaaggtatc agtgaaaaat atcgcggacg tgttttgtct
    43621 ttcttattga tgcatgtagt gacacaacgc atcgatgctc atgtggagca gttgaactgc
    43681 catccattag agatacgccg ctactatcgt cagtttgatt attaggatag actggataaa
    43741 aagaatataa aaaataaaga gagcagcgat aactgctccc tttatttttt taaaatattg
    43801 gcatcaatta cgttataaaa cacatttttt gcattatcta cagttcaact tataatctgt
    43861 ttaatgggac atgtgggtgc caaccatttt ttgttatgca atagtactat ttagttgttg
    43921 catggatccg tagtaaaagt ccaagtacaa cgagttgcgc cagtttacat agcgttttgt
    43981 atccagaatg ttgactaaaa caaaagttcc tatgtcaacc gtcaacagag acaatctgtc
    44041 cgttgcagga cctgacagat ttagaaatgt tcaatctgtc tcttatgtat tttaaatcaa
    44101 tactcactag gagaagtttc ctaacctcct aatagtaatg ctggtagtaa cattgtcaaa
    44161 actgcttcct cttaggaaca agcaaagcat aacgagtgta aaaataaaat gtagactatt
    44221 gtaacatatc attgattatt tcaagtaaaa acgataaaag aagtaaacaa acacttaaaa
    44281 acaatggaaa ttagtcccaa ctttaagaaa tgtatactat aatggaaatt atcaattatt
    44341 tatttggagg aatatccatg aaacaaaaaa tcgcagttct tggtcctgga tcttggggca
    44401 ctgctttagc acaaacattg gcagaaaacg ggcatgatgt ccgtatttgg ggaaatgtac
    44461 ctgaacagat tgatgaaatc aataagtatc atacaaatca gcatttttta cctgatttga
    44521 cgatcccaga atcgattatc ggatataaag aattggcaga agcagtggat aacgcagatg
    44581 ctattttatt tgttgttcca acaaaagcga ttcgttcagt cgcacaggaa cttattggta
    44641 aaatgaatac taagccaatc attatacatg caagtaaagg actagaacaa gatacgcata
    44701 aacgaatctc agaagttttg gctgaagaaa ttccagaaga aaaaagacag gcaattgttg
    44761 tattatctgg tcctagccat gctgaggaag tggcagtaca tgatatcaca acaattacag
    44821 ctgcaagtat tgatcaaaaa gcagcagcct atgtacaaaa actgttcatg aatgattatt
    44881 tcaggattta tacaaataat gatgtgattg gcgtagaaac tggtgcagca ctgaaaaata
    44941 tcattgcaat tggtgccggc gctatccatg gattaggatt cggagatgat gcaaaagcag
    45001 caatcatgac tcgaggattg gcagaaatca gccgtcttgg tgtcgcgatg ggtgccaatc
    45061 ctctgacatt tatcggtttg agtggtgtgg gggacttgat cgttacttgt acaagtgttc
    45121 attctcgcaa ttggcgtgcc ggtaacttgt taggacaggg acataagctg gaagaagttc
    45181 tagaaaacat gggaatggtc gttgagggtg tggcaacgac aaaagcagca gtagagctag
    45241 ctcaacagct aggtgttgag atgccaatta ctcaaacaat ctataatgtt ttgtataatg
    45301 gagaagatat caaaaaagca gcaaaagaaa tcatgttacg tgacggaaaa atggagaatg
    45361 aattttctct tcgttaaata gatgatcaat agttaatcgt
//
LOCUS       DANKAZ010000013        44619 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000013 DANKAZ010000000
VERSION     DANKAZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..373
                     /locus_tag="PWY67_000671"
     CDS             <1..373
                     /locus_tag="PWY67_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2056979.1"
                     /translation="RSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSEGNRRMYS
                     LNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQIFRDEILA
                     QGGLSKTGHYQSQGLQRQFFD"
     gene            401..1741
                     /gene="glnA"
                     /locus_tag="PWY67_000672"
     CDS             401..1741
                     /gene="glnA"
                     /locus_tag="PWY67_000672"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL2056980.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2934..4544
                     /locus_tag="PWY67_000673"
     CDS             2934..4544
                     /locus_tag="PWY67_000673"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL2056981.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            4836..5705
                     /locus_tag="PWY67_000674"
     CDS             4836..5705
                     /locus_tag="PWY67_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL2056982.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            5870..7141
                     /locus_tag="PWY67_000675"
     CDS             5870..7141
                     /locus_tag="PWY67_000675"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2056983.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7138..8433
                     /gene="rho"
                     /locus_tag="PWY67_000676"
     CDS             7138..8433
                     /gene="rho"
                     /locus_tag="PWY67_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL2056984.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8532..8795
                     /locus_tag="PWY67_000677"
     CDS             8532..8795
                     /locus_tag="PWY67_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL2056985.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9240..9689
                     /locus_tag="PWY67_000678"
     CDS             9240..9689
                     /locus_tag="PWY67_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2056986.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9746..11164
                     /locus_tag="PWY67_000679"
     CDS             9746..11164
                     /locus_tag="PWY67_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2056987.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11164..12024
                     /locus_tag="PWY67_000680"
     CDS             11164..12024
                     /locus_tag="PWY67_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL2056988.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12198..12971
                     /locus_tag="PWY67_000681"
     CDS             12198..12971
                     /locus_tag="PWY67_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2056989.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13118..13483
                     /locus_tag="PWY67_000682"
     CDS             13118..13483
                     /locus_tag="PWY67_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL2056990.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13631..14839)
                     /locus_tag="PWY67_000683"
     CDS             complement(13631..14839)
                     /locus_tag="PWY67_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2056991.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(14858..15136)
                     /locus_tag="PWY67_000684"
     CDS             complement(14858..15136)
                     /locus_tag="PWY67_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2056992.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15290..15985
                     /locus_tag="PWY67_000685"
     CDS             15290..15985
                     /locus_tag="PWY67_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2056993.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16427..17224)
                     /locus_tag="PWY67_000686"
     CDS             complement(16427..17224)
                     /locus_tag="PWY67_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL2056994.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17221..18405)
                     /locus_tag="PWY67_000687"
     CDS             complement(17221..18405)
                     /locus_tag="PWY67_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2056995.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18430..19677)
                     /locus_tag="PWY67_000688"
     CDS             complement(18430..19677)
                     /locus_tag="PWY67_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2056996.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            19892..21637
                     /gene="dnaX"
                     /locus_tag="PWY67_000689"
     CDS             19892..21637
                     /gene="dnaX"
                     /locus_tag="PWY67_000689"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL2056997.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21657..21971
                     /locus_tag="PWY67_000690"
     CDS             21657..21971
                     /locus_tag="PWY67_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL2056998.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            21996..22592
                     /gene="recR"
                     /locus_tag="PWY67_000691"
     CDS             21996..22592
                     /gene="recR"
                     /locus_tag="PWY67_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL2056999.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22656..23300
                     /locus_tag="PWY67_000692"
     CDS             22656..23300
                     /locus_tag="PWY67_000692"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL2057000.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23315..23644
                     /locus_tag="PWY67_000693"
     CDS             23315..23644
                     /locus_tag="PWY67_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL2057001.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23658..24596
                     /gene="holB"
                     /locus_tag="PWY67_000694"
     CDS             23658..24596
                     /gene="holB"
                     /locus_tag="PWY67_000694"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL2057002.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24632..25456
                     /locus_tag="PWY67_000695"
     CDS             24632..25456
                     /locus_tag="PWY67_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL2057003.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25449..25796
                     /locus_tag="PWY67_000696"
     CDS             25449..25796
                     /locus_tag="PWY67_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL2057004.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            25865..26737
                     /gene="rsmI"
                     /locus_tag="PWY67_000697"
     CDS             25865..26737
                     /gene="rsmI"
                     /locus_tag="PWY67_000697"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL2057005.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            26846..27967
                     /gene="dinB"
                     /locus_tag="PWY67_000698"
     CDS             26846..27967
                     /gene="dinB"
                     /locus_tag="PWY67_000698"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL2057006.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28021..28623)
                     /gene="nrdG"
                     /locus_tag="PWY67_000699"
     CDS             complement(28021..28623)
                     /gene="nrdG"
                     /locus_tag="PWY67_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL2057007.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(28938..31127)
                     /gene="nrdD"
                     /locus_tag="PWY67_000700"
     CDS             complement(28938..31127)
                     /gene="nrdD"
                     /locus_tag="PWY67_000700"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL2057008.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31426..32220
                     /locus_tag="PWY67_000701"
     CDS             31426..32220
                     /locus_tag="PWY67_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2057009.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32210..34252
                     /locus_tag="PWY67_000702"
     CDS             32210..34252
                     /locus_tag="PWY67_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057010.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34533..34682
                     /locus_tag="PWY67_000703"
     CDS             34533..34682
                     /locus_tag="PWY67_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL2057011.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34693..36207
                     /gene="dltA"
                     /locus_tag="PWY67_000704"
     CDS             34693..36207
                     /gene="dltA"
                     /locus_tag="PWY67_000704"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL2057012.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36204..37409
                     /gene="dltB"
                     /locus_tag="PWY67_000705"
     CDS             36204..37409
                     /gene="dltB"
                     /locus_tag="PWY67_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL2057013.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37454..37687
                     /gene="dltC"
                     /locus_tag="PWY67_000706"
     CDS             37454..37687
                     /gene="dltC"
                     /locus_tag="PWY67_000706"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL2057014.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37690..38958
                     /gene="dltD"
                     /locus_tag="PWY67_000707"
     CDS             37690..38958
                     /gene="dltD"
                     /locus_tag="PWY67_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL2057015.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39099..40181
                     /locus_tag="PWY67_000708"
     CDS             39099..40181
                     /locus_tag="PWY67_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL2057016.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40181..40984
                     /locus_tag="PWY67_000709"
     CDS             40181..40984
                     /locus_tag="PWY67_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2057017.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41054..41794
                     /locus_tag="PWY67_000710"
     CDS             41054..41794
                     /locus_tag="PWY67_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2057018.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            41805..42809
                     /locus_tag="PWY67_000711"
     CDS             41805..42809
                     /locus_tag="PWY67_000711"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL2057019.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(42854..44551)
                     /locus_tag="PWY67_000712"
     CDS             complement(42854..44551)
                     /locus_tag="PWY67_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057020.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14324 a         7801 c         9303 g        13191 t
ORIGIN      
        1 ccgatctatg tccgtatttc caattggtac agttatgaag ctgactgatt tgtctgctcg
       61 gcaaattcgc tattatgaag aacaagattt agttcatccg gaacgaagcg aaggaaaccg
      121 acgtatgtat tcgctgaatg atattgatat gttgcttgag attaaagact atctttcaga
      181 gggattgaat atggctggga tcaaacgtgt atacgaaatg gcacaggcga aaaaacaaga
      241 agcaaaaaac aagaagccgt tgacagatca tgacgttcga caaatttttc gtgatgagat
      301 tttggcgcaa ggtgggttaa gcaaaacagg tcattatcag tcgcaaggat tgcaaagaca
      361 attctttgac tgaataaatt aaaaaaggaa gagggatacc atggtaaaga aacagataac
      421 aggtgaagaa atcaaacgaa tcattgagga agaaaacgta cgatttttac gattgatgtt
      481 cacagacatc ttaggtacga tcaaaaatgt agaagttcca gtgagccaga tcgacaaagt
      541 attagaaaat aaaatgatgt ttgacggttc ttcgattgaa ggatttgttc gaattgaaga
      601 aagcgatatg tatttgtatc cagatctctc cacttggatg atcttcccgt gggaaagtgc
      661 tcatggaaaa gttgctcgtt taatttgtga tatctataat ccggatggga caccatttgc
      721 cggcgatcct cgcgggaatt tgaaacgagc attgaaagat atgcgcgacc ttggttttac
      781 ctctttcaat ttaggacctg aaccagaatt cttcttattt aaattagacg aagacggtgg
      841 gatcacaacg accttgaatg ataaaggtgg ttattttgat tttgcaccaa cagatttagg
      901 tgaaaattgc cgccgtgaca tcgtgttgga attagaaagt ctaggctttg aagtggaagc
      961 ttcacatcat gaagtagcgc caggacagca tgaaattgac tttaaatatg cagatgtggt
     1021 agatgcttgt gataatatcc aaaccttcaa gctcgttgtc aaaacgattg cacgcaagca
     1081 tggacttcac gcgactttca tgccgaaacc gttatttggg atcaatggtt ctggtatgca
     1141 ttgcaatatg tctttgttca atgatgaagg aaatgttttc tatgataaag acggtgaatt
     1201 ggaactaagt gaaacagctt atcatttctt aggtggtttg ctgaaacatg ctcgtgccta
     1261 cacagccgtt tgcaatccaa cagtcaattc ttataaacgt ctcgttccag gttatgaagc
     1321 gcctgtttat gtcgcatgga gtggccgtaa tcgttcgcca ttagtacgtg taccagaatc
     1381 tcgcggttta tccacacgtt tggaattgcg gtcagttgat ccatctgcaa acccatactt
     1441 ggcaatggca gtattgttgc aagcaggact agacggtatc cgtaatcaga tcacaccgcc
     1501 agcagctgtt gaccgtaaca tttatgtaat ggatgaggaa gaaagagaag ctgcacatat
     1561 ccaggactta ccttcaacta tccataatgc tatcaaagaa ttgcgtaagg ataatgtaat
     1621 gattgacgca ttaggacaac atatcttctc aaattttgtt gaagcaaaac gtctcgaatg
     1681 ggcggcattc agacaaactg tttccgagtg ggaaagagaa caatatctag aactttatta
     1741 aaacaggaat accgtgaatt agaggctgtg aaatcagcgt tttgacctga gtattagaga
     1801 aactaacgga aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
     1861 ctgattgaac accgtttagt ggagaggttg tgaaaaagct gccctgcaac aagtaataag
     1921 aatagccaag caaaaacagc ttttcttgtt tttgaattgg ctgaacttat taccgcagat
     1981 gcaagctttt gaacaccgtt taccaagagg ttttgaaaaa gacgtttagt ttcgagtatt
     2041 aagaaaaatt ttgtgaaaat ggctttctac atttttgcat aaatttggct taataccgag
     2101 aaactggctt ttgaataccg tttatccaga ggttgtgagc ctaccattta actccgaatc
     2161 acaagaaata atttcaaaaa cagcttctca tgtttgatgg atattgtgac ttgtgaccga
     2221 ggagttggca ggagaacacc gtttatttag aaataaggag gcacgacttt tgtcgcatct
     2281 ccttattttt tacttatgtg attcgttacc ttcaatgaat ccctacaagg cacttttgtt
     2341 gaaaaaaagg tcatctttga aaaaattagt ggaattgttt tgtttttgat attatttaat
     2401 agaaaagata tgccaaaaat ccgagaactg tggactacgt tttttacgta aaggaggtaa
     2461 ataagggtga aagaaaaaga ttgtttctac gataagctat ataaactgtt gctgcaagta
     2521 ggaaaaagta gttctttgtt aggtgggata ttatctatca taatgtttgt tttatttttt
     2581 tatctattat ttcacttaaa atttttcagt aacgattttc tgatttgctt aggtttaacg
     2641 ttagctatgt ttatatcaag cttattatgt gggttaaatt ttttttgcac tcataaagga
     2701 cttaagaaat aatttctcaa aggatatcga aaaagaaaaa attgtaagcg gatagtattc
     2761 atttgagtac ttgactttcc cttagtcagc ttgtattata caccttgggc acccttttag
     2821 agggtcagaa gtttacagaa atttcggctc cctattctta ttgaagaata gggagctttg
     2881 tttattgtct gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa
     2941 aatatatttt tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt
     3001 ctttaggacg cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat
     3061 acatcaacgt ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag
     3121 atgatggtgc tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt
     3181 tgaacaaata ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac
     3241 gtaagggcga atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca
     3301 aagaaaaaat catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag
     3361 ggggaacagt aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag
     3421 cagatgtcgg tacagataat gttatgtata tccatacaac attgattcct tacttgaaag
     3481 ctgctggaga aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag
     3541 gtatccaacc gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa
     3601 acaaattggc acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag
     3661 atacacttta ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg
     3721 accatttaaa actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac
     3781 gtgtcttgaa cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac
     3841 cagatgcata tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag
     3901 acatcgagat cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt
     3961 tgaaagacgc agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga
     4021 aaatcgaagc gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg
     4081 gtatgcagat ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat
     4141 cagctgaaac gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa
     4201 acatcgagaa tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag
     4261 gaacgaagac ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc
     4321 gctatgagtt caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag
     4381 gtgtctcacc agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg
     4441 tgggatgcca attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca
     4501 aagcatttgt cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag
     4561 aaagagaaaa gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct
     4621 gatttgttat tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg
     4681 ctattggata ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt
     4741 agtagaaaac taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca
     4801 aatttagttt tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat
     4861 ttttaaaagc tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg
     4921 aatggactca agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa
     4981 catcaatggg tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg
     5041 atttagttga agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt
     5101 acgaagctgc tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac
     5161 atctaccatt tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa
     5221 aaggtatctc agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg
     5281 gtaacggcga attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact
     5341 acttagcttg cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag
     5401 cttttgacca cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac
     5461 atggcggatc aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa
     5521 aagtaaatgt taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg
     5581 aagctggtaa agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta
     5641 aagcagcaat cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag
     5701 cgtaagcatc cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct
     5761 ctccattctt ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata
     5821 aaatacaagc atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat
     5881 cgtaatcaaa ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag
     5941 tgcagtcgca ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt
     6001 gccggacata caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt
     6061 ccatttttcc aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc
     6121 gcacggaaaa atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa
     6181 atttggcgaa gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga
     6241 cctacacgtc aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat
     6301 gtatttacgg acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc
     6361 agtcggagca acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat
     6421 tgaaaacgct gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg
     6481 ggcaaaagtt cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca
     6541 tggctgtcgg catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc
     6601 agctgcagta ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt
     6661 tatagcaaaa ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt
     6721 ttacccctcg aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc
     6781 cacagatctg cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat
     6841 catcgatacg atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc
     6901 tgatattacc gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac
     6961 agaagtcgtg gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc
     7021 agaagggaca acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat
     7081 cgagaaacta acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg
     7141 agcgattatt tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat
     7201 gccaaagaat ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct
     7261 gtgattcgtg cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc
     7321 gttggtcaag atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat
     7381 atctatattt ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca
     7441 ggaaaagccc gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc
     7501 gttaatggaa aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct
     7561 ctttatccgc aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg
     7621 atcgatatct tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa
     7681 gcagggaaaa cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa
     7741 gtagaattaa tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga
     7801 agcgtgaaag gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga
     7861 gtttctgaat tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt
     7921 atcttgatgg atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc
     7981 ggacgtacat tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc
     8041 ggggcagcaa gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc
     8101 gatacaggaa gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta
     8161 gaattacaac tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa
     8221 aaatcaggta cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg
     8281 aaacttcgaa aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt
     8341 ttgcgtaaaa caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt
     8401 aaaaaaaatc cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag
     8461 atgttaatgt tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga
     8521 gcgaaaaaat catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt
     8581 caacaactgg tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat
     8641 gggaagacgg taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat
     8701 tctacactgg acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa
     8761 aatacggtct caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt
     8821 agcagactat ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg
     8881 tttgatgctg aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag
     8941 ttttaattat ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg
     9001 aagtaaaaaa ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt
     9061 gttttgttat agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca
     9121 cgcggatgat gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat
     9181 gctatactga cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta
     9241 tgaggtttga tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa
     9301 aaggatcatt atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg
     9361 ttcaaaaagt cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag
     9421 atggatttgg gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt
     9481 ttcatacagc caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt
     9541 ttgccatcgt agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct
     9601 ccagacgatt gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag
     9661 atatcatcag acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata
     9721 gtgaagaaaa aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg
     9781 aattgctcat acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca
     9841 tcaagcaaaa ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat
     9901 caaagaggca gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc
     9961 cgggaaaaaa gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat
    10021 agacgaagca ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc
    10081 aaatgagaaa aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc
    10141 atttgtgatc gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc
    10201 tgatcccaac tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga
    10261 tacagcgttt gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg
    10321 aaaagcaggt tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg
    10381 ttttcttggc ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa
    10441 taaaattagc gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt
    10501 atcgatctta attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt
    10561 gttggatgct ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg
    10621 attacttatc gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgacttc
    10681 gactttctcg atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc
    10741 tgcaggaatg acacctcccc taggtattgc tttagccact ttattattca aacataaatt
    10801 taccaaagaa gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac
    10861 agaaggtgct ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac
    10921 aggttcagca gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca
    10981 tggagggatt tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt
    11041 ggcaattgca tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt
    11101 gaaagagaaa aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc
    11161 gtaatgttat atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta
    11221 ccagccttca atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga
    11281 ttagaagcac cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag
    11341 tttgttgcgg cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg
    11401 gatcatggcg gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg
    11461 atgatcgatg cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt
    11521 gcgttagctc atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat
    11581 ggttctgctg aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt
    11641 tttgtgaaag aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg
    11701 tatccgaaaa acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa
    11761 atcgatcttc catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa
    11821 gcagtaaaat acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc
    11881 aatgctttga gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat
    11941 ccagcagcta ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca
    12001 gtaggcaagg ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag
    12061 tcttgtcata ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct
    12121 tcttatttct cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa
    12181 gtaaatgggg tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt
    12241 atctaaaaaa caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg
    12301 aagccacgat taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc
    12361 gcggtggcgc catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca
    12421 ccaaattaga agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc
    12481 gagatgatat ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc
    12541 tagcaagatt tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga
    12601 gtgaatatac aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa
    12661 caatcaacgg ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa
    12721 tgagttgtcg cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa
    12781 tggtcaaaaa agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa
    12841 aaattgcaca taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga
    12901 caagtaaaga actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc
    12961 tgtacggata aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct
    13021 tatctttgct cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata
    13081 atcttacttt tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt
    13141 agtgaaggca atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt
    13201 tagctttatg gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg
    13261 ggatctgttg atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc
    13321 agctgcagtt atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat
    13381 gaaagtattg attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt
    13441 gctaagtcaa ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat
    13501 tcttatcgat aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa
    13561 ctatttttac tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta
    13621 ttcatcaaga tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac
    13681 tatgttttcg gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa
    13741 tcacatacaa tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa
    13801 cgcctaagcc taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt
    13861 cgcggacgat ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc
    13921 cgactaatgc tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata
    13981 atcctgctgt taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga
    14041 aaatccttcc agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa
    14101 atgacccagc agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac
    14161 ttgaataaga aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa
    14221 taaaactatt gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat
    14281 tttttacagg aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc
    14341 agataatgaa acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag
    14401 cggcagctaa actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag
    14461 ggatatatgc agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat
    14521 tcaagaatcg aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg
    14581 aaaggagata gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact
    14641 gtttccccat gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac
    14701 ctgcttggct caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca
    14761 acagataata aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat
    14821 taaataattt cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt
    14881 tttgtatttg aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa
    14941 taaataaatc tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat
    15001 tctcatccac tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta
    15061 aagaagcttt catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt
    15121 tcatccagta attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa
    15181 cgaataaaaa aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta
    15241 atatcattag taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt
    15301 agatgaaagt ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga
    15361 taaaaatgat tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat
    15421 ggaagattcc catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa
    15481 tgatgggaga gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa
    15541 agacgaatat tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc
    15601 agagttttat cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct
    15661 acaacactat gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca
    15721 gcaaatgcta ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga
    15781 tttattcggt gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga
    15841 aatcgccaaa ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact
    15901 gaaaaaagat ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt
    15961 gttagaagac aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct
    16021 gagtattaga gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat
    16081 accgagggtc agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc
    16141 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    16201 tgaccgagga gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc
    16261 atcaagtaat aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact
    16321 tatcaccgta gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac
    16381 gatttttgtc acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc
    16441 cgccaccgcc aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc
    16501 caggaggtgg gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg
    16561 ttttttcagt taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt
    16621 tcaattgata acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa
    16681 caacagcaat gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt
    16741 cttcgtctac acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag
    16801 acaggtaagc ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat
    16861 ctaagatcga atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa
    16921 tgtagatttc acgctgtccc atatctaata gcaatactga gccattttga tcatttccga
    16981 ctgcgtctct taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg
    17041 ttacgatcaa gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag
    17101 attggatttg atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat
    17161 gagtagccac cggccagcca acacttacta gcaaaagaaa gaataaaaaa aactttttca
    17221 tcatatcaag taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc
    17281 ggtcagtccc aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc
    17341 attcattgcg tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca
    17401 taccggcagt aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc
    17461 ggtcaatgtt gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa
    17521 ttcagattga atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa
    17581 cccggctaaa tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc
    17641 ttcgaccatc ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga
    17701 aagcttgcct gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat
    17761 ccgccatatg cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt
    17821 agcccaataa gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc
    17881 tttgggaaca aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt
    17941 ctcgacagca aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag
    18001 tacgacggga ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat
    18061 ctctgcacca cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc
    18121 gtccgctgta tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat
    18181 atcggcaagc tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt
    18241 gctttcttcg ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc
    18301 ttctgtatag atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt
    18361 caacggaccg ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt
    18421 ctaaataaat taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt
    18481 agggatgccg ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt
    18541 aggctgaacg ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt
    18601 ttctgtcccg caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt
    18661 ttgtttttct gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata
    18721 agaaccattt gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc
    18781 attttttccg gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac
    18841 gctaggatca cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc
    18901 ggtgtaagaa atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca
    18961 agaatcatcc aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata
    19021 agaaatccgt tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa
    19081 ttcagctaaa tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat
    19141 tgcgtttgta taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg
    19201 taagaataat tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa
    19261 tttgattccc tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac
    19321 gccgccaaat ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa
    19381 catggaagga gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat
    19441 gatttttcca ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc
    19501 gtctgttaaa acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac
    19561 tttgacacct tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag
    19621 ccattgatct gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa
    19681 aaattcctcc tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg
    19741 aagatgtgaa acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa
    19801 atacctgaaa aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag
    19861 atagtgaatt ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg
    19921 gcgttcgcag cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa
    19981 tgctattgtt tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg
    20041 aaagacgagt gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg
    20101 tgagccatgc aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt
    20161 gatcgaaatc gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg
    20221 ggcaaattat gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat
    20281 gctttcaaca ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt
    20341 catattcatc ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac
    20401 tcagcgattt gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat
    20461 cttgaaagaa tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc
    20521 agaaggcggg atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg
    20581 aacaattact ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga
    20641 cagttacttg agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca
    20701 aattctcgca gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg
    20761 tgacttgctg atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag
    20821 aagtacattt acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa
    20881 gattttgagc gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga
    20941 agtcgctaca gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc
    21001 atttgcagat gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga
    21061 aatcaaggaa ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca
    21121 aacaaatcat aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt
    21181 tctgcgggaa gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct
    21241 gatgagtcta tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg
    21301 acctacagga ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga
    21361 cgaagaattg cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc
    21421 tgatcctgtt tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca
    21481 ggcacaagaa gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga
    21541 gttgcttcct caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact
    21601 gtttggctcc gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga
    21661 tgcgtggaat gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa
    21721 tggcagaagc acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat
    21781 tggtcaaagc ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag
    21841 tcgttgatcc tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg
    21901 cgatcgcaca aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc
    21961 caggatttta gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga
    22021 aactgatcga cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg
    22081 ccttttatac gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa
    22141 gtgtaaaacg cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct
    22201 gcgaaatatg tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga
    22261 aagatgtaat ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg
    22321 gtgtgttatc accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc
    22381 agcggctgca tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg
    22441 gagaagcaac agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc
    22501 gtctagcaca tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat
    22561 tgaaagctgt cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac
    22621 aatataaaag cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg
    22681 aggggcctga tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag
    22741 aagtagcaaa aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag
    22801 agatccgagc agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac
    22861 tgctttatgc agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa
    22921 aagggaaaat cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag
    22981 gccgacgcat cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta
    23041 cgccggattt taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga
    23101 aaaacagaca aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg
    23161 tacgtcatgc ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg
    23221 caagaaaaag ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc
    23281 cgcaattttt tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca
    23341 agacaaagac agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa
    23401 actatcatcg acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga
    23461 agatgaccgt gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca
    23521 atttgtatct acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt
    23581 agaagtagaa gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt
    23641 ttaggaggag ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt
    23701 atcagcagct tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag
    23761 gggagaaagg aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta
    23821 cccaaatgaa agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga
    23881 aagaacatcc agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga
    23941 tcagacgttt gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt
    24001 tgatcaaaga agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg
    24061 aagagccccc tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc
    24121 ctactattca atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga
    24181 tccatcgatt gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa
    24241 caaacagttt aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg
    24301 acttgattta tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg
    24361 tccaaaaaaa attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg
    24421 tcttgatgtt ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca
    24481 aagtgaaaca gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca
    24541 gcaatgtcag ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg
    24601 ccgcaaagga tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag
    24661 agatgcaggt catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa
    24721 ggtattagtc gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga
    24781 gattgatccc aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc
    24841 tgaattagaa aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa
    24901 agaaaaagtg cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga
    24961 tcgaaataaa ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt
    25021 aaaagatctg gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga
    25081 tgaagcaaag ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt
    25141 tttaggcgat ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa
    25201 tccaacaaaa atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga
    25261 agaatacgag acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga
    25321 tggtaaaggg aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac
    25381 tggacgggaa aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg
    25441 tgagatgaat ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga
    25501 gaaatacatt aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa
    25561 atacaacttt ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag
    25621 ttgcacaagc tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg
    25681 aaaaaattta tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca
    25741 atgacgagcc ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa
    25801 taacaagagg agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg
    25861 acagatgtat aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt
    25921 accgacgcca attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga
    25981 atccgatctt atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga
    26041 aatccataca ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt
    26101 gattgctttg ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc
    26161 catcagcgat cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat
    26221 ctctatccca ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca
    26281 accattttta ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa
    26341 aaagctaaaa gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc
    26401 tacattgacg aattttttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt
    26461 gacaaaggtc catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga
    26521 agaacatcca gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc
    26581 agtgacggaa gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc
    26641 ttcaggcgaa aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa
    26701 acaagaagtc taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg
    26761 aaagactaat gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt
    26821 cttgtataat gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac
    26881 atcaagaaaa atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga
    26941 tcatcctgag tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg
    27001 gcgtggagta gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat
    27061 gagtgcacaa aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca
    27121 taaatacagc gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt
    27181 ggaaccagtt agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa
    27241 gtcagccatc aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac
    27301 atgttctgcg ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa
    27361 acctagagga atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc
    27421 aattgaaaaa tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat
    27481 ttatacagga aaagacctat atgaatgcac agaaatgatg ttgattcgaa attttggaaa
    27541 aatgggctat tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac
    27601 aagagagcga aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga
    27661 agcagtgctg actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt
    27721 ccaaaaacat gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac
    27781 caaaagagtg acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag
    27841 ccttatatgg gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt
    27901 gacgaatctg gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga
    27961 aatataaaaa cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt
    28021 ttatatcaag tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc
    28081 ccaaatcaca acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct
    28141 acttccgcga aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag
    28201 gatatcgatt tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa
    28261 ggcatagcct gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg
    28321 tacgacttgc agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc
    28381 ttgcacgtaa tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg
    28441 tttgccatat cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca
    28501 gccactaacg taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta
    28561 gtcagcaata tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct
    28621 cataaaaaaa cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact
    28681 cgaccacata aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat
    28741 tttgatagag ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg
    28801 tcatagcctc ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc
    28861 aactaaacga aaaatatgag gaactatttt tgtttagcta ttcttattac ttgatgcagg
    28921 acagcttttt cacaacctca tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg
    28981 ccatgtacca tcggacgagc ttgcggattt cctaaatagc cacaagtacg tttgactaca
    29041 tcacatgttt ttggatcatg atttccacat tctggacatt cgaaccctcg ttctgtcggt
    29101 ttgaaatcac cttcgaaacc acattgatag caatgatcga ttggcgtatt cgtaccaaga
    29161 tacccgattt tgtcatatgc aaaatcccat acagcttcta aagctttcgg attttgttgg
    29221 agcatagggt attcacagta gtggatgaat ccacctgaac aatattttgg ataatccttc
    29281 tcaaattcca atttttcaaa tggcgttggg tttttacgca catcatagtg gaaactgttt
    29341 gtataatatt ctttatctgt gatattttcg atgatgccga atttttctgt atccaaacgg
    29401 cagaaacggt cagttagact ttcacttgga gttgaataga cactgaaatg ataaccatat
    29461 tcatcccccc atgcatctgc attagctttt aattctttga cgatatcaag tgtgaaagct
    29521 tttgcttctg ggtttgtttc ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat
    29581 ccaatgtagc ctaacgatac agtagctcga cggtttttga ataattcatt gacagaatcg
    29641 gtacgtgaaa gacgtttccc gaaagaacca tacatatata aaattggcgc atttgctgga
    29701 atggcttctt tacaacgttc tacacgatag agaagcgcat ctttcatgat ttctaagcgg
    29761 tcagacagta actgccagaa tttattctga tctccttttg attcgatcgc gattcttggc
    29821 aaattcaatg taacaacacc taaattcatt cgtccgacat ttacttcttg accattttcg
    29881 tctttccacc cttgcaagaa tgaacgacag cccataggta ctttgaaact tccagttaat
    29941 tcaacgattt tatcgtaatt cagaatatca ggatacattc ttttcgttgc acattccaat
    30001 gctagttgtt tgatgtcgta attaggatct tcagggttta agttcacacc ttttttcaaa
    30061 gtgaagatca gtttagggaa gatagctgtc cgcttttcac tccctaatcc attgattcga
    30121 atctgtaaaa ttgctttttg aatttctctt tcaaaccagt tttctcctag tccaaatcct
    30181 aatgaagtga aaggagtttg tccatttgaa gtaaataagg tattgatttc atattccaaa
    30241 ctttgcatag catcgaagat atcttttttg gtttttgctt ttgcatattc tttttggcgt
    30301 tctggcgaat cgatccattc ttccgcgtct tttagatgtt ttttatagtt caattctgca
    30361 aaaggagcaa gcaattcatc tgttcggtcg gcagaacagc caccatattg acttgacgct
    30421 acattggcaa taatctgtga gatttgtgct gtggcggtct ggatagattt tggcgattcg
    30481 acttcggcat tgccgatttt gaacccattg ttcagcatgc ctttgaaatc aatcaagcag
    30541 caattcgtca tcggtgtata aggatgataa tccaaatcat ggtaatggat atcgcctttt
    30601 tgatgcgcat ttgctatatg tggtggcaac attttcagtc cgattgattt tccaacgatc
    30661 ccggctgtca gatctcgctg tgtattgaaa acattactgt ctttatttgc gttttcgttc
    30721 acgacagttt ggtccttgtt gattagcttt tcaatcgaaa agttgatatc tgttgcttta
    30781 ctgcgagcaa aatctcgttt ggtacgataa ttgatgtact cttctgctaa ttcatattcg
    30841 tttttttcta gtaatatatg ttccacgatg ttttgaatct catagatttt gacattttca
    30901 gcaaaccggc ttttgatttc ttggacaacc aagtctgcaa tcatttgaat tttttcataa
    30961 gtaagtggtg ataatgagcc gtgaattttc ttttctgctt tgatcagtgc ttcattgatc
    31021 ttttgttcat cgaaatcaac atggcggcca tcgcgtttga tgactttcat ctgggatgtc
    31081 tctggctgct gagtaacgac agttttttct tcataattta ctttcattat cttcaactcc
    31141 tgctaaattg ttcttgtcta tcataaaaca aaatcgggga aggagcaact aaatatcggc
    31201 tatatatagt attcaatgtg ctgtataatt ggatttctat actatatgta gtgtatattt
    31261 tattgtgaaa aaaggtgaaa acttgtagct ggtggatttg ttttttgcat atttaatttt
    31321 gctgaataaa ataattgaat aaaatttttt attcagtcca agtaaaagtg acaaaaacat
    31381 aagagcaaat tattcgtaga tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga
    31441 ggaggagaag aattggaaaa gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc
    31501 aaactttctc ccgtcaaagc cttagataat atctcattca cagtagaaga aggcgagttt
    31561 gttgggatta tgggcccaag tggtgcagga aagacaacgc tgatgaatat cttgtccacg
    31621 atcaatttgc ctacgatggg cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa
    31681 aacagtgagc tcagtgactt tcgtcgaaaa aaactaggat tcatttttca agagttcaat
    31741 ttgatcgata ccctaaatgc aaaagacaat atcctattgc ccctagcagt tgaacggatg
    31801 actaaagagg aaatggaaaa gagagtaaga catgtagctc aattgttgaa tatagaagaa
    31861 ctgctcaaac ggtatcccga tgaactttct gtcggacaga gacaacgaat tgcagctgct
    31921 agagcacttg ttgttcagcc acaagtgatt tttgcagatg aaccaactgg ctctttggac
    31981 tcgaagtcgg ctacagaatt gttgaactac ttgaagacca tgaatcaaaa agaaaaagca
    32041 acgatactgt tagtcaccca tgatccatat acagcaagct actgcgatag aattttattt
    32101 attaaagatg gtgtgatttt ttctgaagtt gtcagaagag ggacaagaag agagttcttt
    32161 gagaaagtaa tagacatgca ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa
    32221 actagccgtt aaaagttttt ttcggcagtc acgaggctat cttgtttatt ttttcagttt
    32281 gactttatcc gtaatgatct attattcctt cagcgcaatg acctacgatc aatctttagt
    32341 cagaagagca agtcaagata cttcgataga tggtggattg agttttggtg gatttatcat
    32401 tactgttgtt ttgctttttt tcgtattcag tgccaaccga tttttcttga atagaaggca
    32461 aaaggaaata ggcatctatc agctttttgg tatgaataaa ctgcagatat caggtatata
    32521 tgtgttagaa atcatgataa ttggattgtt cgcctgtatt tctgggatat tactcggtat
    32581 tattttttcc aaactctttt caatgatttt ggttcgaatg atggatatgg atcttactag
    32641 tccatttttt atctccattc catcagtagt agatacttta tttgcttttt tcatcatcct
    32701 tttagcagta tctgtatatt caatatggaa aatatggcgc tatccaatga ttcgaagttt
    32761 tggagaaaaa gaacaagcag atagttcaac aatgcgcttc aggacaaggc accgattact
    32821 cgcagtcatc gggttactac ttttaacgag cggatatttt ggagctagtc attttaggga
    32881 aatcacttca tggatgatca gttatcatta tcttgataca ttagttttct tttttccttt
    32941 tttaattttt ttcgtttgtg tggtcggtac ttatttgttt ttctgttata catttcggtt
    33001 atttttgatc atattgagta aatggaagat ttattactat ggtatcaatt ttttgataat
    33061 gggtaacacg caagttcatt tgctcaaaag ttggcgtaca aattctctga ttacagtcat
    33121 tctcggaatt tctttaatga caattggcgg cacggctagt atctctacta ttttatccta
    33181 ccaggagaaa gtttcctccc cgatggacta tcagtttgat gttgagacag agaaaaaaat
    33241 caagccgatt ctagcagagg aagggcagaa gataactaaa gaaatcacct tgcattataa
    33301 agtcattggg agtatctata gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct
    33361 gatcacggaa acacaatatc aagccttcag aaaagttaat ccagatttgc cgaacatttc
    33421 tttgaaatca cctggtcata ctgttttgct tgatcaaatg caaactcttt ttcggaatat
    33481 ttcattatat ggatctagta ttggactgcc agataatcaa gaattgaaga ttcaacaaat
    33541 gcttccaagt gtattaggtg acgaaacaat ggtatatcta agtcctgtac tgattgtaga
    33601 tgataccgtt tttgaaaaag caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc
    33661 aggtggaaat aatgaaaaaa tagatgaacg actgagcaat gacctgtcga taaagtggct
    33721 caatgctgtt tattattctt atgatatcgt aaatggaagt ctacaaggag aaattagcac
    33781 aaagaaactt ccggatgacc aaatgaagga agcacaattt agccaagaaa gctctcgttt
    33841 gaatttctct tccagctatt cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat
    33901 ttttgtcaca ctatttgtcg ggatgatcgt gattattgct actggtagta ttttgacagt
    33961 ccggcaatta gcagaagcag aggaagaaaa agaaaattat cacctattga ctaagctggg
    34021 gattccacaa agaaggatca gacggatgat cttcgagcaa aacgcactta ccttctttcc
    34081 acccatgata ttagggatca ctcacgcagt atttgcaatc aatgtcttca ctcagtatat
    34141 aaaaactgcc gattactggt tggcttatct tgtgagtggc ttattgatcc ttatctatct
    34201 tttgctttat gtcattacga gtcagttata ttgtcggatt atcgaggaat gatatacgga
    34261 ataagacaag ataataaata gaactaaatt ttgaatacca tttatttgga ataagaggtt
    34321 gtgacacgat ttttgtcaca acctctttat tttttatatt ttttaaaagt acgagaaatt
    34381 ataaagaaaa aagcagtgat ttgatgtgct atttttataa aagctatgtg acaaaactgt
    34441 tagtaaaaaa acggtttctg taagctagac acatgtttct ttttgtcaaa attagttaca
    34501 ctttgtgtga ggttatatga ggagggaatc caatgaacaa atggcgttct aatccaaaat
    34561 tgaattattg gggaaaattc atcattcgaa caatttttta tagtgcagta ctgctgttcc
    34621 tgatttatct gtatcactat aaaaatattc aaggaggaac ttttatctat aacgaatttt
    34681 aggaggaaga gtatggaaat caaaacgatt attgaagcaa tcgatgattg gggaataaat
    34741 gaacctgatc gagttgctta tcaggcggat gagacgcata cttttggtga attgaaacaa
    34801 gcatcagacg cacttgctta ttatttagaa aaaaaggtag aaggcgatgg accaattgtc
    34861 gtttttggca atttggagtt tgaaatgatt gtctcatttc taggggttgt caaatctggc
    34921 catgcctatt tgccgattga agagcataca cccaaggagc gtatcctatc gatctttcgg
    34981 gtggctaaac catctatggt catcagtatc ggggactgga tagaagatat tccttcggtt
    35041 cctgtcataa caaaaaaaga atttcaagaa atcaagaaga taccagtagg attttatccc
    35101 agaaatagcg taaaaggtac agaaaatttt tatattattt ttacttctgg tactactgga
    35161 gaaccaaaag gcgtacaaat cagtcatgat aatctagtca gctttgttca atggacgatg
    35221 gaagactttg ggatcaagcc tggtatgcat ttcttagccc aagcgccatt ttcttttgat
    35281 ttatcggtat tttctattta tcctgcatta gtttctggcg gaatgctgaa gccgttaaat
    35341 aaagcagttg ttcaagactt tcgtcaatta ttcgcgacat tgccagcctt gaagttgaat
    35401 gtgtgggttt ctactccatc atttatggat atttgcttga tggaacctac attcaatgga
    35461 gaaaatgtgc cggaattgga catgttttta ttttgcggag aagaattgac gaaaaaaact
    35521 gcagaaagtc tacttgatcg attcccaaat gcacggatat ataatactta cggtccaaca
    35581 gaagcaactg tagctatttc aagtattcag attgaccagc atgtactaga tagttatgac
    35641 cgactaccga ttggctacgt aaaaaaagac acgaaagttt ctattgtcca agaaggtaaa
    35701 cctgtttcaa aaggagaaac gggtgaaatc attattgcag gaccaagcgt atcaaaaggt
    35761 tacctgaaca atcccgaaaa gacagctgct gcgttctttt gtcaaaatga cttagcaagt
    35821 taccatacag gggatgcagg tagactagat gaatatgggc ttttatttta tgaaggacgt
    35881 atggatcagc aggtcaaatt acatggatac cggattgaac tcggggatat cgaacattat
    35941 ctgctccaag acaaccgaat caaacaagct gtcgtcgtac caaaatacca aggaacaaaa
    36001 gtacagcagc tggttgcttt tgtcgtatta gaaaacaaaa cgaaacagcc ggattttcag
    36061 ttgactaaat ctatcaaaga acaattacta gaagttgtaa tggattatat gattcctcag
    36121 aagttccatt atgtcgatct gctgcctcag acagttaatg gaaaaatcga tcgaaaaaaa
    36181 ttaacagctg aggtgaatcc tgaatgatgt tacctcatat gatcccttat gcagagccgt
    36241 tttatttttt tcttttagcc attgcgcttg taccgatcgt tctatcttta ttgatatggg
    36301 aaaaaagatt accagtttat caatcagcag tgacactttt ctttttattt gtcagctttg
    36361 gaggtaatta ttggcaacag ggagcggcgc tgattgggta tgttttgtgg cagacgatac
    36421 ttgtatgggg ctattttgtt tatcggcaaa ggaaaaatca gacaggatgg ttttttgccg
    36481 ccgttatttt agcgataatt ccactgtttt taacgaaagt gactcctttt tttgaacatg
    36541 gaaaagcatc gttacttggt tttttaggaa tctcttattt aacctttaaa tctgtacaaa
    36601 tgatcatgga aataagagat ggcatgatca aaacatataa gccatttcgt tatatccagt
    36661 ttctgctgtt ctttccaaca atctcttctg gaccaatcga tcgctatagg agatttgaaa
    36721 aagatcagct ccatccgcct gttcgtgata aatatgttga tcttttggga aaaggcgtcc
    36781 aaaatatttt tgtcggtttt ttatataaat tcattatcgg ttattatttt ggtcaagtac
    36841 tgcttcctgt agtgggacgc attgccttac agcatggtgg tatttcatgg gcattagtag
    36901 gttatatgta cgtgtacagt atgtatttgt tttttgattt tgctggctat agcttatttg
    36961 cagtcggtac aagctacatg atgggatatc agacaccaat caatttcaat aaaccttttt
    37021 tatcatggaa tatcaaagaa ttctggaatc gttggcatat gacgctgtcc ttttggttcc
    37081 gtgattatgt atatatgcga ttgatgttct tcatgatgaa gaaaaaattt tttaaaagca
    37141 agatcgtaat gtcaaatatc ggttattttg ctttgtttct tctaatgggt atatggcatg
    37201 gattgacgtg gtactacatc gtatatggac tgtatcatgc ttgtctgatc tgtctgacag
    37261 atgcatggat ccgatttaag aagaagcata aagagcagtt gccttctaac aaatttacgc
    37321 atggcttagc cgtatttttg acatttcaag cagtatgttt tagcttcctg attttttcag
    37381 gatttttaga taaacttttt tttagctaac gaatagtgac atattaattt atataacaaa
    37441 ggagaaaagg aaaatggaag aacaaatttt atcaatctta gaagaaataa caggaacaga
    37501 tgaagtaaga aaaaatcgtg atattgatct atttgatgaa gggttgcttg attctttagc
    37561 atctgttcaa ttattagtcg agctagacgg agagctggat atccaagtcc ctgtatctga
    37621 attcgagcga gaggattgga gcacaccgaa taaaatcatt caacaagcaa ctgcattgaa
    37681 gggttgagta tgaaaaagaa actttttgct attttcggcc cggttgtttt agctgttggc
    37741 ttactgtttg tgttttttac ctcctcctat caagtagata tctctaatcc agaaacgatt
    37801 agacgtgcct cttcttccat gtcagtgaac gtgttgaaag gagatgcgat aaaaaattat
    37861 gcattatctg aaaaacaata tattcctttt tttggctcaa gtgaattaag cagaatcagt
    37921 ccatttcatc cgtctgttct ggcagaaaaa taccagcgaa actatcgtcc atttttgctg
    37981 ggagcacccg ggacacaatc gttaagccaa tatatgatga tgcgttctgc aggagatgct
    38041 atgaaaaaca agaaagtcgt ctttattatt tctcctcagt ggttcgttaa aaatggtgta
    38101 aaaactgatt actttaatac gtattattct gaacttcaaa catacgactg gttgttttca
    38161 atgaagaaag taacgcctgc tgatcgctat ttagctagaa gattattaac tttctcaaaa
    38221 gtaaaagaga atgatacgtt gacggcgatt ctgcaaacga tcaaaaaagg gaagctacct
    38281 ttacctgaat ctttgaatca gctgcgcagc caatggaaca tgctgaagag agaggatgaa
    38341 gtattcagca atattgggtt gaaagatcga cagcaaaaaa tcgatcatga gtcaaaacga
    38401 ttaccaaaac aataccaaga aacagaatta tccattttag caaatcaaat tggagaacga
    38461 gaaacgacga ataatccatt cgggttgaaa aacgattttt atacgcatcg tatccgagcc
    38521 cacgagccag agctcaaaca gtcacaaaaa aattgggatt atcgtttttc gccagaattt
    38581 tctgattttc agctggtctt ggatcaatta gcaaaaaacc ataacgaagt attgttcatc
    38641 atcccaccgg ttaacgagaa atggagcgat tataccggac tatctcaaga aatgttacaa
    38701 ggatttgcca aaaagatcaa atttcaatta aatagccaag gattcaatcg aatagctgat
    38761 tttgtcaatc aagcgggtac caactatttt atggaagaca cgatccatct aggctggaaa
    38821 ggctggttgg ctgcagatca acagatcaga ccgtttttag aagaaaatca tattactgct
    38881 agtaagtatc atcttgacga tgcttttttt agcaaatctt ggcaacatca aataccagat
    38941 aaactacaat taaaataaag aaaacaggcg agtgttgctc gcttgttttc tttatttttg
    39001 aatattctga ataaaagata aaaataccaa gtttgcctga acaaaaatca taagcaatgg
    39061 tataatcaca atatgaatat agaaatgggg gaaaacattt ggatacaaaa gaagaacagc
    39121 tacttattga tttaacttct gcaaaaggag tacctggaaa tgaagaagag gtaagagaag
    39181 tattcagaga atatgccaaa ccattcgctg atgatatttt ttatgacggt ttaggcagtg
    39241 tgattgccaa acatggtgct ggcggaggcc ccaaggtctt tatttctgga catatggacg
    39301 aagttggttt tatggtaaca aaaataaccg aaaaaggatt cttggaattc caaactcttg
    39361 gaggttggtg ggggcaagtc atgcttgctc agcaagttga aatcaaaaca cgtgaaggga
    39421 aaatcgtcca cggtgtgatt ggttccaaac caccacatgt tttaacacct caagtaagaa
    39481 ataaacctta tgaaataaag gatatgttca ttgatattgg cgcatcaagc caagaagaag
    39541 ccaaagagtg gggcatacgt cctggagata tggtcactcc atatattgaa tacaaacgaa
    39601 tgaatggcag taaatattta ctggcaaaag catgggataa tcgtatcggg acagctgttt
    39661 ctctgcgagt attagagaat ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa
    39721 gcgatgtgca agaagaagta ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc
    39781 ccgatattgc ttttgcttta gatactggta cagcaggaga cactccagga atgacaccaa
    39841 aagaagcaga ttcgatttta ggaaaaggcc cacagatttt gatttttgat gcatctatga
    39901 tcccgcataa aaaattattg aattttgtta ttgatgtagc agaggaaatg gatattccat
    39961 tccaatatac agtgatttct ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag
    40021 atggtgttcc gtctgtagcc attaccgttc ctgttcgtta tctgcattcc catacatcag
    40081 tgattcatga agatgattat ttgaatacag tgaaactagt cacagaatta gtgaaacggc
    40141 ttgatagtga aacagttgaa aaaattcgga gttatgaata atgaataata gaatacctat
    40201 ttctgaatat cggaaaaaag cacgagtttc tttggaaaat caatggggaa tcaatacttg
    40261 gcttgttttc ttatctataa tatttacgag cgtcatccag aacttttttg acggtttcag
    40321 tccttttccg gaagaaagca tgcagcagca cttattgagt tttctagtcg ataatttact
    40381 tttattcgca ttcaactatg cattgtttta cattgcgcta gtagttgtac ggggtggcag
    40441 accaaaaagt aaccttttat tttcagtttt ccaaagaaag tattttggtt cgatacttgg
    40501 catcaatttg atcaattcag tcatcaactg gctactcaac agtttaatct ttctgcccat
    40561 gtttttaatc agcggagtga gtacttatat gcagctgctt ttcagttcaa atgcggtacc
    40621 tacgggtgtc atgccagaag attttcttga tatcagcttt gttttatcga tgatagggat
    40681 gatttttgtt tcagcactca tcatgagtat tgttggcggt ttattccaat ttgctgcatg
    40741 gacaaagttc gactatcctg atttgtcggt tattcaatgt ttgaaatatg gctggttttt
    40801 attgaaagat agaattggta cgtatatttt gttgcagctg tcttttatcg gctggtattt
    40861 gctaggagca atcgctttgt tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc
    40921 agttgcagaa ttttatgaac aagcacggat tgaaaaagga ggtccagacg cctattttac
    40981 ttagcatgta agcagtttat tcatcaaaaa aataactttt gtggtaggat aaatctttga
    41041 gggggaatgt acgatgaaat taactgtttt aggatgctta ggagcgtatc cttataaaaa
    41101 tcaagggaca acaggttatt tgcttcaatc tgacgggttc aatttgttga tcgatgcagg
    41161 tagtgctacg ctgatcaaat tacaagaaca ccttgatcca cttgatctag atgctgtgat
    41221 ccttagccat tatcatcatg atcatattgc tgaccttggt gtattacagt attactggca
    41281 acttcatcct aagagaaatc cggagacatt gctttcaatt tatggacaca caaaagattc
    41341 cattcacttt gaagaattga cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc
    41401 tgaagaatta aaaattggtc cttttctgat tacttttatg gaaacaatcc atccagtagt
    41461 atgttacgcc atgcgtatcg tagaagaaac aacggggaaa gtattcgtat ttacaggtga
    41521 ttcaggttat ttggaaagtt tcgctgattt tgcgaaagat gctgatttgt ttctagcaga
    41581 tacctatctt tttgaaggaa atgaacatca tcatgcccat ttcacttcaa aagaagctgg
    41641 agaacttgcg aaatcagcta acgtaaagaa acttgtactt acacacctac ctcagtttgg
    41701 tgatttagaa caattgaaaa atgaagcgca aaaggcagca ggcaaaggga ttgatataga
    41761 acttgctgca gttgacaaag tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa
    41821 atgaaaacca cgacccaaga atcaatttag caattgaaca atatttgctg caagaaatgc
    41881 ctttagatga acctattttg ctcttttata tcaatgaacc ttcaatcatc atcggcagaa
    41941 accaaaatac gcttgaagaa atcaatcgag attatgtgga agaaaatggc attcatgttg
    42001 tccgtcgctt aagtggaggc ggagctgtct accatgattt tggaaacctg aactttagtt
    42061 ttatcatgcc tgatgatggg gattcattcc gtgactttgc gaaagtaaca cagccaatca
    42121 tccaagcact tcatgaattg ggtgttgctg gggcggaatt gaaaggccga aatgatctag
    42181 tcattgatgg catgaaattt tctggcaacg cgatgtatgc taccaacggt cggatgtttg
    42241 ctcatggaac aattatgttc gactctgata tcaatgaagt agtcaatgca ttaaaagtaa
    42301 aaaaagacaa aatcgaatca aaaggaatca aatcgatccg ttctcgtgta acgaatatca
    42361 aaccatttct tccagaagaa aagcaaaaaa tgacaacaga agaatttcgt caagaaatct
    42421 tgttgaaaat tttcggcgta gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg
    42481 aagattggaa aaaaatcaat cgaatctctg aggaatatta tcgaaattgg gattggaatt
    42541 acgggaagtc tccagatttc aatttctctc gccaaaaacg tttttctatc ggttcgattg
    42601 aagtccattt gaatgtctca gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct
    42661 tcggactagg agatatctct gacgtggaaa aaaaattggt tggacagaaa tatgataaag
    42721 catctctatc aaaaatagtc agccaaattg atgtgaagaa atattttggt gcgatagaac
    42781 cagaagaatt gcttacctta ctttattgaa aataaaaaat agcggaatcc acatatatgg
    42841 attccgctat tttttataca ggattcgggt aatttcgaga gagttgaaca gtgatccgat
    42901 caatgaaagt atctttttcc tgcgttagtg ttcctgtgta acgatccgct acgtctttca
    42961 tcatcattaa gtcaaaacgt tcctgatcat ctaattcttc tgtgatgcct cgactaaata
    43021 ggtttgtatc gtaatgtgca ctatgttcgc aacaaatgac gaaatgttcc tcttgttcgt
    43081 gtatcgacaa ataaattttt cgatacagtg gattttcgat ggcatgagtt tctctaaagg
    43141 aatgctctaa agcgtggatc aaaagttgag ccagatcgtc atcgttgata ggaagttctt
    43201 cgggaagatc tgcttggaag gttatctgaa tattccgttt ggcagctaac ttctggtaac
    43261 gcgctaagat aagatttgtc acctggtggt tgcaaaaaga acacagcatt tcgaaatttt
    43321 ttgcttcttc taatatcttt tccacatagt ttttagcttt tgtttggtcc gtatgcatta
    43381 aatcttgcag attctccatc tcttggacaa attccaggcg aagtgtattt acttcttgga
    43441 tatgctgtcg aagctgctca taatgttgct ccaacaaatc atttttgatt tttaagaaat
    43501 ctacttgtct ccgatctttg tcggtattta agatgtgatc gattagattg tagctgataa
    43561 taacgattag aatgataata tataaaatcg tagaccaatt gatcgttgtt tgatagatac
    43621 ctaatgccgt ttggatgtaa atcaagcaat ggataatggc taccaagacg atccaaggtg
    43681 tacaagctac ataaaaacgg ttattcttat atgcttcgcc aatgcatgca agtgaagtga
    43741 ctaaagtgct tataacacta gcaatattag caatcggcat taattcagga agttctgcag
    43801 gagtaaaagt tgcataaagc agaacgccga taaaaatcaa aagttggaac gtacaaaaaa
    43861 ttccgtacca ttttttatag taaaacattc ggctccaata gtagcagatg atgaaagctg
    43921 acgttaagat aataaataaa tgagataaaa aagaatgaac gaatggttca aaaagaatac
    43981 cacttaaaat atcttctgat acagcttcaa atcctaatgc caaagcaaag caacttaaga
    44041 gaataagtga gtattcaaga cgtttttgtt tatacatcaa gtagatcgta tagaaaaagg
    44101 tccctatagc gataaaaaca gcgattatca tcgtcaatac ttgcggcact gattgtgaat
    44161 aaataaaact aatcaatggt ccagttgttc ctagataaac tttcggtggc agtccagcat
    44221 agttctgata aggagatgag actgtaaggg tcaatttttt tccgatataa tttgctggta
    44281 aatctatcac agccaatgag agtccgggat tgctttcttc ctgataatct ttccgttggt
    44341 ataaaatctt ttcgcctaga tatacagtaa tccattgatg gtttcctttg attagtaaat
    44401 ttgcttcgtc caacggtttt tcaagtgtcg tttccataat gaatgtttcg tctttatcca
    44461 cggtaggaat cgtacgagtg tacttgctgt caaacttaaa atcaggatca tcttttgtat
    44521 agaaaaccca cgaatctttt atttctttca tttgattgct gtccgcagga gaaaaaataa
    44581 aataaaagaa ataagcaatt cctgttaaaa ataacaatg
//
LOCUS       DANKAZ010000014        43701 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000014 DANKAZ010000000
VERSION     DANKAZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(340..2721)
                     /locus_tag="PWY67_000713"
     CDS             complement(340..2721)
                     /locus_tag="PWY67_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2057021.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2711..3358)
                     /gene="recU"
                     /locus_tag="PWY67_000714"
     CDS             complement(2711..3358)
                     /gene="recU"
                     /locus_tag="PWY67_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL2057022.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3422..3961
                     /locus_tag="PWY67_000715"
     CDS             3422..3961
                     /locus_tag="PWY67_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL2057023.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4051..4470
                     /gene="gpsB"
                     /locus_tag="PWY67_000716"
     CDS             4051..4470
                     /gene="gpsB"
                     /locus_tag="PWY67_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL2057024.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4492..4859
                     /gene="rnpB"
                     /locus_tag="PWY67_000717"
     ncRNA           4492..4859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PWY67_000717"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5068..6234
                     /locus_tag="PWY67_000718"
     CDS             5068..6234
                     /locus_tag="PWY67_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL2057025.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6280..7776
                     /locus_tag="PWY67_000719"
     CDS             6280..7776
                     /locus_tag="PWY67_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL2057026.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7857..8540
                     /locus_tag="PWY67_000720"
     CDS             7857..8540
                     /locus_tag="PWY67_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2057027.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9185..10375
                     /locus_tag="PWY67_000721"
     CDS             9185..10375
                     /locus_tag="PWY67_000721"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL2057028.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10477..11430
                     /locus_tag="PWY67_000722"
     CDS             10477..11430
                     /locus_tag="PWY67_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL2057029.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11499..12848
                     /locus_tag="PWY67_000723"
     CDS             11499..12848
                     /locus_tag="PWY67_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2057030.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13031..13354)
                     /locus_tag="PWY67_000724"
     CDS             complement(13031..13354)
                     /locus_tag="PWY67_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057031.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13483..14646
                     /locus_tag="PWY67_000725"
     CDS             13483..14646
                     /locus_tag="PWY67_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2057032.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14716..16065
                     /locus_tag="PWY67_000726"
     CDS             14716..16065
                     /locus_tag="PWY67_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2057033.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16110..16541)
                     /locus_tag="PWY67_000727"
     CDS             complement(16110..16541)
                     /locus_tag="PWY67_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2057034.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16929..19571
                     /gene="alaS"
                     /locus_tag="PWY67_000728"
     CDS             16929..19571
                     /gene="alaS"
                     /locus_tag="PWY67_000728"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL2057035.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19771..20367
                     /locus_tag="PWY67_000729"
     CDS             19771..20367
                     /locus_tag="PWY67_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2057036.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20489..21637
                     /locus_tag="PWY67_000730"
     CDS             20489..21637
                     /locus_tag="PWY67_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2057037.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21675..22088)
                     /locus_tag="PWY67_000731"
     CDS             complement(21675..22088)
                     /locus_tag="PWY67_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL2057038.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22167..22598
                     /locus_tag="PWY67_000732"
     CDS             22167..22598
                     /locus_tag="PWY67_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL2057039.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22697..22930)
                     /locus_tag="PWY67_000733"
     CDS             complement(22697..22930)
                     /locus_tag="PWY67_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL2057040.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23113..24783
                     /locus_tag="PWY67_000734"
     CDS             23113..24783
                     /locus_tag="PWY67_000734"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL2057041.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24930..25316
                     /locus_tag="PWY67_000735"
     CDS             24930..25316
                     /locus_tag="PWY67_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057042.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25303..26001
                     /locus_tag="PWY67_000736"
     CDS             25303..26001
                     /locus_tag="PWY67_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2057043.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26006..26470
                     /locus_tag="PWY67_000737"
     CDS             26006..26470
                     /locus_tag="PWY67_000737"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL2057044.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26467..27378
                     /locus_tag="PWY67_000738"
     CDS             26467..27378
                     /locus_tag="PWY67_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2057045.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27613..28152
                     /gene="pyrR"
                     /locus_tag="PWY67_000739"
     CDS             27613..28152
                     /gene="pyrR"
                     /locus_tag="PWY67_000739"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL2057046.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28166..29449
                     /locus_tag="PWY67_000740"
     CDS             28166..29449
                     /locus_tag="PWY67_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL2057047.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29462..30388
                     /locus_tag="PWY67_000741"
     CDS             29462..30388
                     /locus_tag="PWY67_000741"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL2057048.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30407..31687
                     /locus_tag="PWY67_000742"
     CDS             30407..31687
                     /locus_tag="PWY67_000742"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL2057049.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31692..32768
                     /gene="carA"
                     /locus_tag="PWY67_000743"
     CDS             31692..32768
                     /gene="carA"
                     /locus_tag="PWY67_000743"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL2057050.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32773..35958
                     /gene="carB"
                     /locus_tag="PWY67_000744"
     CDS             32773..35958
                     /gene="carB"
                     /locus_tag="PWY67_000744"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL2057051.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36012..36788
                     /locus_tag="PWY67_000745"
     CDS             36012..36788
                     /locus_tag="PWY67_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL2057052.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36785..37717
                     /locus_tag="PWY67_000746"
     CDS             36785..37717
                     /locus_tag="PWY67_000746"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL2057053.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37714..38430
                     /gene="pyrF"
                     /locus_tag="PWY67_000747"
     CDS             37714..38430
                     /gene="pyrF"
                     /locus_tag="PWY67_000747"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL2057054.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38437..39126
                     /locus_tag="PWY67_000748"
     CDS             38437..39126
                     /locus_tag="PWY67_000748"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL2057055.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            39099..39368
                     /locus_tag="PWY67_000749"
     CDS             39099..39368
                     /locus_tag="PWY67_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057056.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39406..40047
                     /locus_tag="PWY67_000750"
     CDS             39406..40047
                     /locus_tag="PWY67_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2057057.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40090..40782
                     /locus_tag="PWY67_000751"
     CDS             40090..40782
                     /locus_tag="PWY67_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL2057058.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40779..41681
                     /locus_tag="PWY67_000752"
     CDS             40779..41681
                     /locus_tag="PWY67_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2057059.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41839..43545)
                     /gene="efbA"
                     /locus_tag="PWY67_000753"
     CDS             complement(41839..43545)
                     /gene="efbA"
                     /locus_tag="PWY67_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL2057060.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        14005 a         7700 c         9490 g        12506 t
ORIGIN      
        1 aactcaaaat gaagccttct ttcaaaaaat tattcaaaac catatattgt attttttatt
       61 tgataataac aatgggatta ttaagaagtg gcactaaata aaagactagt gtgtatttag
      121 ccgatttaat tataaacaag taaatggata aagtatatgt aatgataaaa gaaaaatgga
      181 ttttctaatt tttaagctaa atgttagatc acacatgtat atgctggata gtcttataaa
      241 aaatcatgta atacagcttg gtacatgatt tttatttgta gctgtttttt acatagacac
      301 aaaaaagaaa ccacgcttgt agacgtggtt tcttttttac tatctgtttc cagaagctga
      361 ggtgctgctg gatggtgtat ggtcatttgc agcatccgct ccgctagttg ctggctcact
      421 ggaagaagca ggaggagcag atgaagacgg tgtcgagctt tcttcggatg aagaagaagg
      481 aaccgttgtt gaagacgctg gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc
      541 agcagttgat tcgctttgac tagtagagct agagctttga gtcgttgtct ctgttgttga
      601 gcttccagga gtctggacat agctggaaga tggaatagtt gttgatggtg taattgcatt
      661 cgatctagct gtatattgat ctttgtagta cagttcaccg cccacacgaa ttaatccgct
      721 tggcatctcc cagtctgtat ttgtcacgtt agctgaaaca tactgcataa gctcgcgata
      781 aacatcagag gctacgtgtg acgattctga agtgacaggt gtcatttttt tattgtaacc
      841 tgtccacacg gaaatcgaat aatttggcgt atagcccgca aataggatat ctggatacac
      901 cccagaagaa ataccaagtt ttgcatactc gtcatctgtg taattggatg ttccggtctt
      961 accggcttga tataacccag cgatttgcgc attcgtacca gttccttcag tgatcgtatc
     1021 ttttaaaatg tctgtgatca tataagctgt ttctggagac atggctgttt ttccatctgg
     1081 ctcataagtt tcttctgttc cgtcttggaa gacgatttta ttaacatatt gaggtttgta
     1141 ataagtccct ccattggcga aagccgcata ggctgctgcc atttttaagg aagaagctcc
     1201 gtattttgtc ccatcttgtt cttccgtatt actagagatc gcattcgatt gatggatcgt
     1261 actgtattcg atcccaagat tttttaagaa agaagccact ttatctgaac cgacttcatt
     1321 gaataatttg actgctggaa cattacggga taagtaaagt gcttggcgta aagtaatggt
     1381 gcccatatat tgattatccc agtttcctac aggagtcgaa gtcccttcat agttatacgg
     1441 tgcgtcagta atagtttttc cagtagaata tttcaaatat tcaaaagctg gtccgtaatc
     1501 tgtgacaggt ttcatcgtag aaccaaaatc acgggaagta ttcactgcta agttgttgcc
     1561 taaagttacg tcttctgcga tgtgacgtcc gccaatctgg gcttttactt tcccggtatt
     1621 ggtgtcgata agggtggagg caacttgcat ctcgtcgtct gggtaagaga catactgatc
     1681 cgtgttgacg atatcatata gtttcttttg ggcgtccaga tccaaattcg tataaatctc
     1741 taaaccgtct gtgtagacat tcttatctgt tttttcttgt acttcattga tgacttcttt
     1801 gacataatta tcgacgatct tagtattgtc atcggattga gttagttctt gcagaccatc
     1861 cgttaccggt acattaacag cttgatcata ttctgtttga gagatttttt cgttttgaag
     1921 catcgtatac aaaacagtat ctcgtctttt cttcgcctga tctggataaa cataaggatc
     1981 ataggcggaa ggggcttgag gcatcccagc aagcaatgct gtttgcggta aagaaagttc
     2041 tgataatttc ttaccaaagt acatctcaga agctgtctcc atcccgtaca agccgtttga
     2101 catatacact ttatttacgt agtaggtcaa gatctcttgt ttcgattttt tttgttccaa
     2161 tcttacagcc atccacgctt cttgggcctt gcgtttcaga gtctgatcct cggcactggt
     2221 ggagaagaag gaaagtttga tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc
     2281 agaggtaaag ttagagaggg cagaaccgat gatccgaata gggtcaaccc cgatgtgttt
     2341 ataaaaacgg cgatcttcta ccgacacgat cgcatcttct aatgttttcg gtaattcatt
     2401 cgcagaaatt ttttcacgct tttctgcgcc taaatcttcg aatagttcgc catcttgtgt
     2461 atatagtttg gaagatacgg tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt
     2521 tgcgtaatac cagaaaagtc ctacacctgc taaaaagagg atacagaaaa atgagagtat
     2581 tcctaaaagg atcttgataa ataatccttt tttatgtgtt ccagaggact ttccagaacc
     2641 tttgccagaa ttcttcttca cactcttttt aggagtaggc tgtttctgtc gtctagagct
     2701 tctagtttgt tcatttgcca ttcttgcttt ctccttcaat agataatgtt gataaataga
     2761 agtcaaccgc gtccaaataa ggaatccgcg gagaaatacc agttttgatc tctattccta
     2821 gttcttcgat caaagataaa ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt
     2881 aagcaatcaa atgaacacta ttcaaataaa aacagcgctt caaagacgaa aaccataaca
     2941 agacaaagca gaccccattt tgttcgaggc atgctttcat atgttcgatt tgatgggcat
     3001 ggaagttttt aaagggaaaa gatgttttgt tcttggtttc ttttgcttca aagtccaagt
     3061 aaaagcctcg atagacgcca ttatagtctg tagttgaggc ttgggtaaag taggcttcct
     3121 tgatcacagc agcacttctc ttaggatagt ctactttcac gatttggata ggggtaggct
     3181 ttttatggat aaccgccaac ccgcgggaaa gataatattt attactttca ttgattgctt
     3241 cttcaaagcg catcccccga ttgccgaatt cagtgatctg atttcttttt tcttgaattt
     3301 tttcgacggg agaggcattc ttataataag gttggccatt tggatagcgc aataccatct
     3361 ttaatcacac tcctaggcca cattatagca aaaaagcgga tagaaagaaa aggaatctga
     3421 gatggataag tcaaaagtaa tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt
     3481 taaagaaaat gatcctaaaa tccaagttgt taaaaatgtt ttaaaaaaag aactccatca
     3541 gttagcagaa gaagggctgg aatgggtact tgtcagtggg aatttgggcg tcgaactatg
     3601 ggctgtgcaa atagtagcgg aattgaaaga agaatatccg gaactatctc ttggtatttt
     3661 gtatcctttt tctgattttg gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt
     3721 agcagaacaa ttagcagatt atgtcgaagc tgttagccat caaccctacc aatcacctag
     3781 tcagctaaag aatcacactc gttttatact tgagcataca gtgggctgtc tgctgatcta
     3841 tgatgaagaa ttccctggaa aaacgaagta tttttttgaa gatgcacaga attaccaagc
     3901 agatcatccc tacgaaattc ggctgatcag tatggacgat cttcaaaatt ttagtgaata
     3961 atcgatattg tgtttgattt atcaactttt ttcctataca atgaaaaagg tagaaatgtt
     4021 attacgtaaa tgaaaactag aggtgtaaat atggcgaatt tggtttatag tcctaaagac
     4081 attttacaac aagaattcaa aacaaaaatg cgcggctatg atcccgttga agttgatgaa
     4141 ttcttagaca atatcatcaa agattatgaa gcttacagca aagaactcct tgctttacaa
     4201 gaagaaaacg atcgattgag tgcaaaagta gctcaattat ctaaaactca aggagcagct
     4261 caaacacgcg tgcaacaaac agaggcacca aagagcgcag cagtgaccaa ttttgatatc
     4321 ttaaaacgct tgtcaaatct tgaacgtgaa gtatttggta aaaagcttga tcaacaagca
     4381 agtgcagtca aaccagcaca acctaatcca aataactaca caaatgcaga cacaagctta
     4441 gatgataatg aaaaaacacg tcaattttaa tttttgatta atgaatttgc ggttttcggg
     4501 taatcgcggt ctgacttgat caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc
     4561 tgtagtgttc gtgcttagcg aaaccataag ctaaggtact ctttggagta acggcaggaa
     4621 aacaagctaa ggcttcggct atgcttaagt atccttgaaa gtgccacagt gacgaagcgg
     4681 ctggggaaac ccatccggtg gaacgcggta aacccctcga gcgagcaacc caaacaatgg
     4741 taggggcgct ttttgtctgg aagaagaaca ggacaaaaag gcagtataac tgcagataga
     4801 tggttaccgc tttgtttttt ctccctggaa aagcaaagta cagaacatgg cttatagaaa
     4861 accgcaaaca tcagggaatt aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa
     4921 acatttgaaa gctatttttc aatacttcag atgaatcgtt cttattacaa aacaaacttt
     4981 tgtatcaccg gttattcgga atttgagtaa aagtgtgggg taggactgat gtcccacctc
     5041 ctttttttat aaataaggag attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca
     5101 actgcggcaa gcgggctaga agcactggtt ggtaaagaat taagagatct tggaatcgaa
     5161 tgccaagttg agaacggacg tgcacgtttt gaaggaacaa tggaaacgat cgctacagct
     5221 aatttatggt tacgtacagc agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat
     5281 acgtttgatg aattgtttga aaacgtaaaa gcattgcctt gggaagattt tcttccacta
     5341 gatgcagctt ttcctgtagc tggaaaatcg atcaagtcga aactatatag cacacctgat
     5401 tgccaagcga tcacaaaaaa agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca
     5461 gcttctgtac cactgacgga aactggcgca cattttcagt tggaagtagc tttgctgaaa
     5521 gaccatgtga tggtaactct ggatacgaca ggaccaagtt tattcaaacg tggttatcgc
     5581 ttagaaaaag gtggagcacc attgaaagaa aacatggcag cagcacttat tatgttgaca
     5641 aattggcgaa aagaccgtcc attttatgat cctgtatgtg gttcaggtac gctttgtatt
     5701 gaagcagcct taataggcca taatatcgca ccaggattca atcgtgagtt tgcttgcgaa
     5761 tcttgggatt ggttcagtca agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa
     5821 gcagattatg atattgaatt agatattact ggttcagata tcaatggacg gatgattgaa
     5881 attgccaaag ccaatgcaga agaaatcgga ttaggctctt cgattacctt taagcaacaa
     5941 gcagtcaaag acttcaaaac ggataaagaa tacggggtca tcgtggctaa tccgccttac
     6001 ggagaacgtc taggagaaga agaaactgtt cggaaattat ataaagaaat gggcgatgta
     6061 ttccgtccat taaaaacatg gagtaaatat atcttgacta gcgacttagc gtttgaggaa
     6121 ttttacggac aaaaagcaac caaaaaaaga aaattgtaca atggtgcatt gcgtacggat
     6181 ctattccagt attggggaac tcgtccgcct agaaaaaaag agaaagacga ataatcttac
     6241 taattgattc attcaattgg atgtttggag gaatagggaa tgaaagaaaa agagttttta
     6301 caagaagtaa aagaaatcaa tcttctacaa caagctttag gaatcctcga ttgggataca
     6361 caaacaggta tgcctgaaaa agctagtgca taccgaagtg aagtagacag ctatctttac
     6421 agcatttatt tttcaaaaaa aatcggtcca aaaatcaaag aagccattcg ctatttttct
     6481 gaacatccgg aagaattgtc ggaagttgga actgccgttt ttgccaaagt aaaagaagag
     6541 tatgatttgg aacatcgcgt accggaagat cttatgagaa aatatacagc tgccgtttct
     6601 tcagcacacg gacaatggca aaaagctaga gaaacacaac aattttctga ttttcaacac
     6661 gcattgaatc aaaatattga attaacaaaa cagttgatac cttactggag aaaagatgaa
     6721 aagacgaatt atgatgtttt gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt
     6781 gataaggtat ttgatcagct taaagaagga attctttcga tccgccgtac gctccaagaa
     6841 aaagggactg aacctgaaac agacttttta aatcggagaa tgacgaaaaa acaacagaag
     6901 agatttgtag tgaaagtaat cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat
     6961 gacactgtcc acccatttat gactggaatt aatccgaatg atgcacgaat cacgacacga
     7021 tggaacgaaa cagatttcaa aatggctgtt tttggagtga tccacgaggc tggtcatggt
     7081 atgtatgaac aagacatcga tgataaatat gcgtatacac cggtttatga aggtacatca
     7141 atgggtatcc atgaatcaca atcgttgttt aacgagatca ttattggaag tagtcgcagc
     7201 ttctggaaaa agcaatatcc tttctttcaa gaatgtgcag aaggaacatt cgatgatatt
     7261 ccttttgaca aattttatcg ttcattgaac catacaaaat caagtttggt tcgtatcgag
     7321 gcagacagtt tgacttaccc tctccatatc atcattcgtt atgaaatcga aaaaatgatt
     7381 ttcaatgaag gggtagatgt tgctgaacta cctgaaatat ggaatcaaaa atatgaagag
     7441 tatctgggaa tacgaccaac aaatgatgtg gaaggcatcc tacaagacgt tcattggtca
     7501 ggtgcaagct ttggttattt tccctcttat gcattagggt acatgtatgc ggcacaatta
     7561 cttcattctt tgcagcaaga aatagatgtg gaaaatgttc tagcgtcaga cgattattct
     7621 ccaatcaaaa actggatgtc agagcatatc catcaatatg gtgcatctag aaaaccaaat
     7681 caattaatca tggatgcaac acatgaaccg cttaatccgc aatacctgat cgactatatg
     7741 aaagacctgt attttaatgt atatcaggta aaataaaata caatttacaa agatagctga
     7801 aattcatcta atctatagat gttttcagct atttacttaa gaaagaagtg gaacgaatgg
     7861 ataaattaca agtgatcgct gattatagta tggaccaatt agcgcatgat caaacaggac
     7921 atgggagcga tcataccaaa agagttgtca agttagctga aaaaattttg gagacggaac
     7981 cgcaggcaga ccgctttgtg acgcttgcag cagcttacct acatgatacg attgatgata
     8041 aagtcgttaa aaatgagaac gaggcaaagc aacagcttcg tgtattcctt cgctctttgc
     8101 caatagtaga ggaacagatc tcgatgattt tttccattat cgaaaatatg tccttttcta
     8161 aaaatctctc tgaagcggcc gaactatctc tggaaggtaa gatcgttcaa gacgctgatc
     8221 gtatcgaagc attaggagca atcggcattt tgcgtactgc ttatttcggc ggtggacatg
     8281 ggcacccgat ttttgatcct gaactgtatc cgcaaacatt caaggacaaa gaaaattacc
     8341 gaaaaggcac gacagtgatc aatcattttt atgaaaaact gttcttgctg tctgataaaa
     8401 tgaatactga ttacggatat gaagaagcca aacgaagaga aaccttcatg cgtgaattct
     8461 tagaagaatt ttttctggaa tggtctattg acgatgaaaa ttttactcca gacaattgga
     8521 tcaaaaataa caaaaaatga tggaggttgt gacagaagtg gtcagctcca agatatttcg
     8581 gatcattttc cgtaaaagtt acttctggaa cactgtttac caagaggtcg tgagcctgcc
     8641 gttcagctcc gagtcacaag gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat
     8701 tgtaacttgt gattcggaat tggcaggaga acaccgttta ttcggaagaa taggactgtg
     8761 acaagacttt tgtcacagtc cttttttcga attcaacaag taaaactttt aagaaatatt
     8821 cagagagtaa aattaaaaat tgctgataaa cgaagatgga aattttatgt atttttattg
     8881 gtctatgcaa taaataaatt aaatattcag aatacaattg attttaacga aagggcttct
     8941 atcaatggtt gtgttgtatt tatctgccca ggtagagtaa aatagatcat tatctaaaag
     9001 gtaattgttc atactatata agtcatttga aagtaaaaag atactgtaaa taaaaaatgt
     9061 ggttaaataa ttctcataat cagtgtaagc gttatcaaaa atatagcata cttgctttgt
     9121 agatgaatga cacgatatat gtttggtatg ttctgcaaga cagatgaata tggaggtgga
     9181 gacaatgtct tccaaagaac taacaatgta tttagagaaa gaattggatc aactgaaaga
     9241 aaaggggtta tacaatacga tcgacatact agaaagtgaa aatggtgctt gtatcatcgt
     9301 agacggaaaa aagatgatca atctagcatc caacaattat cttggatttg ccaatcgcga
     9361 agaattgaaa aaagcatgta ttgaagcaac cgaaacttat ggtgtaggag caggagcagt
     9421 ccgaacgatc aatggttcgc tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt
     9481 caaagggaca gaagcagcaa tcgcctttca atctggattc aactgcaata tgggcgctat
     9541 ctctgcagta atgacaaaag aagatgctat cttatcggat gaattaaacc atgcatctat
     9601 tattgacggt tgccgcttat ccggagcaaa aatcatccga attaaacatc aagatatgaa
     9661 agaccttgag aaaaaagcaa aggaagcgat agaaagtaag aaatataaaa aaatcatgta
     9721 tatcacagat ggtgttttct ccatggatgg agacattgca cgattgccgg aaattattcc
     9781 gattgttgaa aaatatggac tgatcaccta cgtcgatgat gcgcacggct caggcgtaac
     9841 tggaaaaggt gcaggaaccg tcaagcattt tggattaagc gacaaaatcg atatgcaaat
     9901 gggcacgtta tcgaaagcta taggtgttgt tgggggttat gtagctggat cgaaaacact
     9961 aatcgactgg ttgaaagcta gaagtcggcc attcctattt tctacttcgc taactccagg
    10021 tgcagctgca gctgcattgg cttcgattac tttgatgcaa gaacatccgg aacttgtcga
    10081 aaaagtttgg gaaaatgcta attattttaa agaagaattg aaaaaagtag gctataacat
    10141 cggtatgtct gaaacaccta ttactccagt cattttggga gacgaaaaag tgactcagac
    10201 tttctctaag aagctgatcg aacatgggat ttatgctaaa ccaatcgttt atccaactgt
    10261 gcctcttggg acaggcagga tcagaaatat gccgactgcg gaacatacga gggaaatgct
    10321 tgatgaagca gtagcagttt atcaaaaaat aggaaaagaa atggaaatta tttaactaaa
    10381 tttagttttt tcgtctattt aaaattagag aacatgttca cgcatatatt gttaatcatc
    10441 tgaagatagt agtaaaaaag atcagggagg atcatcatga aaaaagtatt ggtgacagga
    10501 tgtttaggac aaattggttc agagttgatc acacggctac gtcaagaact aggcagtgaa
    10561 aatgtcattg cgacagatat acgcatgcct gaagtcattg agaaaaacga tatctttgag
    10621 atcctagatg taatggatta cgaaaaaatg cgcagcatag cagaacaata tcaagtggac
    10681 acattgattc atttggctgc tcttttgtca gctgttgcag aagcaaaacc acaattagcc
    10741 tggaatttga acgtgactgg gctggtcaat gcattggaag tcgctagaga attccagctg
    10801 aaatttttta cgccaagttc aatcggcgcg tttggtccaa atactccaaa agatcatacc
    10861 ccacaagata ctcttcagcg tcctacaacc atgtatgggg taacgaaggt agctggagaa
    10921 ctactatgtg attattatta tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct
    10981 ggattgattt cttatgcaac cttgccagga ggcggtacga cagactacgc agtcgatatt
    11041 tattattcag caatcaaaga aggaaagtac actagtttca ttgatcaagg tacatcaatg
    11101 gatatgatgt atatgccaga tgcgatcgat gccattataa aactcatgaa tgcagatcct
    11161 gatcgattgg ttcatcgaaa tgccttcaat atatcggcga tgtcttttga cccggaagag
    11221 atcaaagcat ctattcaaaa aatcatacca gattttgaaa tggattatga tgtggatcct
    11281 gtacgtcagt caatagcaga atcttggcca aattcactag atgtttcttg tgcgcaagag
    11341 gaatggggct tttcgccgaa atacgactta gatgtgatga caaaggatat gttggaaaaa
    11401 ctgaaagaaa aattggtaga gacggtataa aacaagaacc acatccaaaa agttttttat
    11461 acttttggga ttggctatgg tatgaaaggg gacaagggat ggaagaatat caagccacgc
    11521 cagtaaaaga agtaaaaata gaaaatgaat taaaacgtac gatgggattt tttactgctt
    11581 tatcgactgt tatgggcaca gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag
    11641 cagaagttac aggatccgcc agtttgcata tgttttcttg gtttctcggt ggtatgattt
    11701 ctgtctgtgc aggactaaca ggagctgaac tggcagctgc tattccagaa acaggaggga
    11761 tgattaaata tatcgaacgg atttacggaa atacagcagc ttttttattg ggctgggcgc
    11821 aagtcgtgat ttattttcca gcaaatgtag cagccttgtc gatcattttc ggtacacagt
    11881 tcgtcaatct atttggtctt tcccagtcaa tgattgttcc cgtagctgtt acagctgctg
    11941 tatcgatcct gttgatcaat tttttaggtt ctaaagctgg aggggctttt cagtcgatta
    12001 cgcttgtctg caagcttatt cctttgtttg tgatcgtcat tttcggtttg ttccgacaag
    12061 aaggagtaga cttccaactg tttccgatcc aagcaggaga aaatctttca tttttctcag
    12121 ccttgggagc gggcttgcta gcaacgatgt tcgcatacga cggttggata cacgtaggaa
    12181 atatttcggg agaattgaaa aagccggcga aagatttgcc aaaagcaatt tctttaggaa
    12241 tcatcggaat catgatcgtt tatttattag taaatgcggt gttcttaaga accgcatcta
    12301 tcgacggagt cgcaggaaac agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg
    12361 gcttcggtgg aagactggtc acagttggta tcttgatttc tgtttatgga accatcaatg
    12421 gctataccct tactggaatg cgtctccctt atgttatggc aaaagaaaac aacctgcctt
    12481 ttagcaagct ctttgctaaa cttcatgata aaactaaagt tcctgtagcc gcaggtatct
    12541 tagagctagt catcgctatt ggcatgatga tgatcggtgg ttttgataca ctaacggata
    12601 tgctgatctt tgtcatttgg attttttata cgatggtctt tgttggtgtc attcttcttc
    12661 gtaaaaaaga acctgatttg tttcgaccat acaaagttcc aatgtatccg tttattccac
    12721 tagtagcaat cattggaggg acttttatcg tatcaagcac gcttatcact cagacctttt
    12781 tagcttcgat ggggatagta ttgacacttg ctggtatccc gatttatctc tatttaaaac
    12841 gtagatagct aaaacattcg aattttatta gaactgcact tctctgccaa acaaaatatt
    12901 ttatttaaat gaggagtacg tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt
    12961 aaatgtcgtt ttgtctacga tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga
    13021 tttctgtctt ttactttgat ttgcgtaact gaactattcg gcgtacgccg cgataaatat
    13081 ttaataaaac caagcaacca gaaatgaaaa tgggaagcca aatcagatag acagggtagc
    13141 caagcacttt agacaaagca tgtggagtca ttttatccat ttcaagaagt gcttgctgcc
    13201 aagtctgaag attgaagact agcatgaaac ccgaaataat caggagaata aatcctaaat
    13261 aatgaattgt agttcttttt cttacataag cgatcgtagc ataaaggaac agtaaaaaaa
    13321 taggtacgcc aaaaacaaag tacatattca gcatctcgac accccctttg tgtttatctt
    13381 actttacttt gaaagaaatg agaaataata gcctcgttac ttgcttgaaa cagcaaagga
    13441 ctgctaagat aagagagata caaagaatca aggaggagtc atatggcaag ttatatcgaa
    13501 ccgattcgtt ttgcgctgat tgtttttcca tttttagcct tagcgatctc atctgttttt
    13561 tttatttatg aatatcgaaa gtatggaaca tttctagtaa caagggcagt tattttgtat
    13621 tctttcgttt tttatttatt gtgtgcgtat tttttggtga tcttaccact tccggcaaag
    13681 gagacggttg cccaaatgac agggccaaag atggagctgc atttatttgc ttcatggcaa
    13741 catttcagag acaacacggt attggtcttg tctgaaccta gtacctacct gccggcaatg
    13801 aagcagagtg tatttttgga accagctttt aatgttttat tactcatacc atttggcatt
    13861 tatttaagat attatttccg gctttctttt atgaaaacga tgattgctag tttctgcctt
    13921 tctctatttt ttgaattgac gcagctaagt ggactttatt ttatctaccc aagaccatac
    13981 cgcttatttg atgtgaatga tttagtgaca aatacattgg gtggaatcat tggttttgtc
    14041 gttacgccgt tatttacatt tatgcttcct acaagaagac gaatggatga actttcttat
    14101 gaaaaaggaa agactgtctc attgactcga cgcttggtcg cttatttgat cgactggaca
    14161 tttttatctc ttattaattt ggtagcggcg attttcttta aaattgcgtt cgattcaggg
    14221 gcagttactt ctcatatctg gtggctttta ttagaagtag gggtatactt tatgattgta
    14281 ccgtttataa ccaatggatg gacacttggc aaaaaaattg tgcgtatccg tttggtggaa
    14341 gaagaccgcg aacgaatttc tttaaaagca ttgatgatcc gctacgggta tctgtacttt
    14401 cttttttatg gtttaagctg gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg
    14461 aatacggtgt tgcagatgag tgcagtattc atttctctcg gtgtcttgtt actacaaggt
    14521 atttttattt taaatatctt gctatcattt ttgagaaaaa aacggattct gttttatgaa
    14581 aaagctagtc acacatatac tgttagcacg attggccaaa caaaggatga tgattctcga
    14641 aattagctga gcaaaaacgc tttgtctgtg ttataatgcc aaagtcgtga taatttaaag
    14701 agaagaggtg cacgaatgaa taattttgat atattccgct tcaaaccgtt tatcaatgaa
    14761 gcgttaaccg caaagggctt caaacagccc acagaggtcc aggaacgttt gattccttta
    14821 attgcaaaag gaaaaaatat cattgggcaa tctcaaactg gtacaggaaa aacacatacg
    14881 tttttgttgc ctttgtttga aaaaatcaat gcagaaaaag aggaagtaca agttgttatt
    14941 acagctccaa gtagggaact agctgcacag atctatgaag cagctacaca aatcgctaaa
    15001 ttttctacac cggagatccg tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa
    15061 ttaaatcgtc tgcatcacca acagccacag gttgtagtag gcacgcctgg tagaatatta
    15121 gacatgatga atgaacaagc gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa
    15181 gcggatatga cattggatat gggtttttta aatgaagtag accaaattgc aagtcgatta
    15241 ccagaaaagc tgcagttttt agttttttct gccactattc cagaaaaact tcgtccattc
    15301 ttacgaaaat acatggaaaa tccaatcatt gaagaaatca aaccaaaagc agttatttct
    15361 gaagacattg aaaattggct gatttcaaca aaaggtaaag atgagaatca aatgatttat
    15421 caactgctga caattggtca tccttattta gcaatcgtat ttgccaatac aaaacagcat
    15481 gtggatgaaa ttgccgatta cttgaaaaac caaggcttga aagtagcaaa gattcatgga
    15541 gacatcaccc caagagagag aaaacgagtg atgcgacaag tacagaatct ggagtatcaa
    15601 tatgttgtag cgactgattt agctgctaga gggatcgata tcgaaggtgt ctctcatgtt
    15661 attaatgcag aaattccaga agatctagac ttctttatcc atcgtgtcgg acgtactgga
    15721 cgtaatggct taaaaggaac ggccattacg ttatattcgc caaatgatga gcaagccatt
    15781 gatgcgatag aaaaattggg cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc
    15841 gtcaaaacat acgatcgtaa tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat
    15901 cccacgctga ttggtcttgt gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa
    15961 aaaatcgatt gggcaattgc agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag
    16021 caaagccgta ctgctcgtaa aaataagaaa aattcacata aataaaaaac aagaggttgt
    16081 gacaaaaggc gtgtcacaat ctcttgtttt taagctagac tttttttcat tccgatacaa
    16141 agtacttcat cttcaataaa aggttctgtg caagtatgat acccttgttt ttcataaaaa
    16201 tctacagccg tcatttcagc tactacataa ctagtctgac aaccttctga aaccgcccgt
    16261 ttttcagcag cttcaagcaa ctgaccgcct aacccttttc ctcggtattc ttttgctacg
    16321 caaaatcgtt ccggcttgaa gcaagtaggt gttttctttt cataacgcaa agtagctact
    16381 ggtctttttt gatcaagaaa tagataatag gtttgttgcg ggatatcttt tccgtcgaat
    16441 tctagttctg caggaatccc ttgttctaat acaaaaactt gatatctcaa aaagcagcta
    16501 gctgcgcgta tccattcatc cgctccaact atttttttca tcatattcct ccttgtcatg
    16561 aatagcagag atgtattgat tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc
    16621 aaaaggaaat gaaaataaaa attgagatat tgacagtgat caatagaatg cgtatactaa
    16681 ttaagtacga aaaggatatg atttgttttt taagactcac agaaaattct ttcttggctg
    16741 aaaaaagaat agtcactaat caaattgctc cctttttcta ttttggtaga aataccgacg
    16801 cagctcagcg ttatcgagtt taagaggtaa tgattacaca tcattgcaat caaggtggta
    16861 ccgcgagttt tcgtccttgg ctgtgatggt gtttttttgt atttaattta tagaaagcag
    16921 gtaatgacat gaaacaatta agtagtgcac aagttcgaca aatgtttctt gatttcttcc
    16981 aatctaaagg acatacgatt gaacctagtg cgtctttgat ccccgtgaac gatccaacct
    17041 tattgtggat caactctggt gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc
    17101 cagaaaatcc aagaatcaca aatgcccaaa aatctattcg aacaaatgat atcgaaaatg
    17161 tcggtaaaac agcacggcac catacgatgt ttgagatgct gggtaatttt tcgattggtg
    17221 actatttcaa aaatgaagcc atccactggg catgggaatt tttgactgat ccaaaatgga
    17281 tggcattcga tccagaaaaa ctttatgtaa cagtttatcc aaaagataca gaagcaaaac
    17341 gcatctggca tgaagaagtt ggcctgccac tagatcacat tattgatgta gaagacaact
    17401 tctgggatat cggtgcaggc ccatgtggtc cggatacgga aattttctat gaccgtggag
    17461 aagcttataa cgatttagca gaagacgatc ctgaaaacta tccaggagga gaaaatgaac
    17521 gttatttaga aatctggaac ttagtatttt cagaattcaa ccataagcct gataacacat
    17581 ttgaaccact tcctcataaa aacattgata cggggatggg attggaacgc atggtctcaa
    17641 tcgttcaaga tgcgtcaacg aactttgaga cagacttgtt tatgcctatt atccacgccg
    17701 tagaaaagct aagcaaacaa gtaaaatatg gtgaaaaccc agtaacagat atttcttaca
    17761 aagtaatcgc agatcatatt cgcgcccttt catttgctat tggcgacggt gcattgccat
    17821 cgaatgaagg gagagggtat gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga
    17881 aaaaattagg tattgaacaa gcattcttat atcgtttagt ccctgttgtt ggggaaatca
    17941 tggtaagtta ttatcctgaa gtacttcaac aaaaagaatt tattgaaaaa gttatccgta
    18001 cagaagaaga acgtttcaat gaaacaatca atgaaggttt ggaaatctta aatcaagtga
    18061 tccaagaagt aaaaaacgaa aacgggacga ctctagcagg aaaagacatc ttcaaactat
    18121 acgacacata tggcttcccg gtagaattaa cggaagaggt ggcagaagat gaaggactga
    18181 aagttgatca tgctggtttt gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg
    18241 cccgcagtac tgaaaaatca atgggggtac aatctgcttt attgacagat atcaaagtag
    18301 aaagtaaatt cattggatac acagaacaag aatcagatag tcaattattg gtcatcgtta
    18361 aaggtgagtc tcttttgaat gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa
    18421 cacctttcta tgcagaaatg ggtgggcaag tagcggatca aggatggatc atgaatgaag
    18481 taggcgaaat cgtagcagtt gttacagatg ttcaaaaagc gcctaatggt caatttatgc
    18541 acaccgtaga agtaaaagca ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc
    18601 aatcgatgcg caatcggatc atcaaaaacc atacagcaac ccatctactg cacaaggcat
    18661 tgaaagaagt acttggcgag catgcaaatc aagctggttc tttagtcgct cctggacatt
    18721 tacgattcga ctttacccac tttggtcaaa tcacttcaga tgaattagct gaaatggaaa
    18781 gaatcgtcaa cgaaaaaatc tgggaagcta tcccagttga aacaattgaa acagatatcg
    18841 atacagccaa aaacatgggt gcaatggctt tgtttggtga aaaatacgga catgacgttc
    18901 gagtagtaaa tattggcggc tggtcaatcg aactgtgcgg aggcacacat gtagcaaata
    18961 cagaaaacat cggaatcttc aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac
    19021 gaatcgaggc tgtaacaagt aaagaagcgt acgaactact tgaagaagaa gaaaaacaac
    19081 taaaacaagt agcaacaatt gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg
    19141 aacaattgca acagcaagta agagagctac aaaaggaaaa tgaacaatta gctagcaagt
    19201 tagcgaatca acaagctggt gatgtattca aagatccaga aacgatcaat ggaaaaacaa
    19261 tcattgcagc acaagtaaaa gtgaaagata tgaatcagct gcgtcaatta gctgaccaat
    19321 ggaaacaaaa aaatatttca gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca
    19381 gtctgctagc ggcaatgacg aaagaatcaa atgaagctgg actaaaagct ggcgacttga
    19441 ttaaagcaat cgcaccgaaa gtaggcggtg gcggtggtgg acgtccagac atggcacaag
    19501 ctggcgggaa aaatcctgct ggtattccag atgcacttaa agctgcgaaa gaatgggttg
    19561 cgcaacaata gggcgtttac gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca
    19621 tgattatggg ggcgaagaca aacaagtctt gccctcatac ttttttcaat tggatggcgc
    19681 atctaatcaa atggtcggat tcggatggtc tttagttgac gaaagtgaca gaaattttta
    19741 cgattagtat aaataaaaag gggtgaaaaa atgaatagta gaaataagac ctttcgacta
    19801 gtcttacgtg cgatcttgtt agcaatcatc atcgtacaag cgatggttcc ttggctgggg
    19861 tttattccat taggattcat tagtttaaca attatccata tcacagtaat tattgctgca
    19921 gtagtattag gcccaaaaga tggcatggtg attggcttgt tttggggaat cgctacaatt
    19981 gttcgtgcct atgccatgcc aacaacgcca tttgatacat tagtgttcac gaatccgatt
    20041 atctcggtag ttcccagagt acttgttggc ttagtagcag gcttggtttt ccactggatt
    20101 tatcaaaaaa ataaatcaat aacgatcagc tcagcctttg caggggtact gggttcattg
    20161 gtcaatacag tcttagtgtt aggattcatg ggattatttt atacaggtgc aacggcagat
    20221 gcctatggtg tagactctgc tcttttattt aaaaccttag caggggttgc agcaatcaat
    20281 gggataccag aagcaatagg tgcgggaatc ataacaccat tactagcaaa agcgttattt
    20341 gcagcaacac cgttgaaacc agaataatga ccatctttta atagtaatag gacgcacgat
    20401 agatagaaaa aggaagagag gagaggaaaa caatcgtcct ctccatcttt ttacctgagc
    20461 aacaagcaaa ctagaagggg taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca
    20521 aataccgcaa cagcggacaa aaaaaggagt actagcgggt atcttaggat tggttacgaa
    20581 tctgatcttg tttgtctcta agctaatgat cgggcttttt tcaggaagtg tctccattat
    20641 ggctgatgca gtgaacagtc tatcagatac tgcttcatcc atattgactt tgatcggatt
    20701 taaaattgct gcaaagccag cagatcagga acatccttat ggacacgaac gcttcgaata
    20761 tatcagtggg ttatttgttt cgatcatcat tacttatgta ggatttcaat ttttagagtc
    20821 ttctgctgaa aaaatcatcc atccagaaaa tgtctcttta acacccatcg tcttttttgt
    20881 cctaatcatt tccatctttt tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca
    20941 atccattgat tctgaaacat tgagagctac agcaacagat agttataatg atgtctttac
    21001 tacactggct gtattgcttt cagcagcaat cgaatgggta actggatggc gtattgatgg
    21061 atatataggt tttgctttag cattatatat cctttatagt ggattgacga tgcttcgaaa
    21121 ttttgtatac gaactattag gaagtcgccc aacgccagca gagattgaag aaatggaaga
    21181 gcatttaaat gcttgcgatg atatacttgg ctatcatgat ttattggtgc ataactatgg
    21241 acccaataaa cgctttgcat cagtccacat cgaagtagat gaatcaatga atttgaatgt
    21301 ggctcataag attattgatc tgatagaaaa agactttaaa aagtctttgg atgtggaact
    21361 tgtctgtcac ttggatccaa tgcctgttga taacgaaaaa tactataata tcttgcggca
    21421 gttaaagcaa attcttcttg aaacaggtac agggcttaat attcatgatt ttagagtaat
    21481 tcatcaagga aaaacgctgc aatttgatgt agtggtccct gagaaattcc agtttgttga
    21541 tacagaacta gaaagaatct tacgtagtca aatagaagaa aaaatcggag attaccaatt
    21601 ggaaattacg tttgatcaca attatctttt atattaaatg agaaactata aaaagactcc
    21661 ttaaagtttt taatttaaag agtctttttt tcttttaatt gtttatggag agcctgacgt
    21721 gcaagattat ctgctccttt atttttgttt tcgggcaacc attgcaggat caataaagaa
    21781 aattcggata gaagttcttg gattctttga aggtagggaa ggaagagctc gttcttcgtt
    21841 gcgtttttgt cgaccgtaga aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca
    21901 ttctgtaagt cgtttttctt tagaaattct aaagttttta aaaagacttc gaactctgct
    21961 tgatggtttg tccctataga aagcgggata gatagctgtt cttgtttacc ttgatagaca
    22021 atcaagattc cacctccact aggaccaggg ttgccttttg tagaggcatc aatgtatact
    22081 tttaacataa aaagagattc tcctttttgt tcaaactaat gttatggtaa gattatatca
    22141 ttactgaatc ggtgagaggt tagagaatga aaaaaaatat tcgatggcag cctgaactgg
    22201 cacatacgat catctattgg tcattgacct tgatcattct gttttttagt ctgattcttt
    22261 ctttagaaaa cacaggtcct tattggaaaa gcaattttgt tttaggtatt tttcttgttt
    22321 ttctatttct aggtttgcgg cgttctttta ttttaaaatc aaactctgtg attatacgtt
    22381 atgctgcttt ttggaaagac cgagaaatat ctgtcggaca gattcgatct ttgacgccaa
    22441 ctaatactgg ggtgcaaatg atgttgcaag atgagaaagc cccatctgtt ttcatgatgc
    22501 gaaaaaaaga tcaagtagtc ctttttgatc attttgaaaa aaacggaccc aaatcagaat
    22561 caattcctta tccttatacg aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa
    22621 aaacgaattt ttacaggaaa agaaaagagt gagacgtacg gttcatcccg tttagcgtct
    22681 cactctattt gtttatctat tcatgcatac tagtatcggt agtctgaacg ttgcctttct
    22741 tgacatgggc agcttgtgtt cctcgattcc cttcgatgat ctcaaattcg accacttgat
    22801 tttcttccaa agttttgaat ccctcttctt caatcgcagt aaaatgtaca aaaatttctt
    22861 ctgtgtcatc ataactgata aagccgtatc cttttttgtt gtcaaaccat tttacaattc
    22921 ctgtggtcaa agcactcacg acctttctaa agtctgatca tagccttcat tataaagagt
    22981 atttataagt acgtcaaata aattaaaaac ctgtaatttt atgttttttt tctgaataaa
    23041 aaaaggtata attggcatat cttaacaaag aagatatttt gttgaataaa gttcgtgaaa
    23101 aggggtaatc aaatgaagaa tgatctgcaa atttcacaag aaacagagct agaaccgatt
    23161 gttgaagtgg ctagtaaatt agatcttaag gaagacgatt tggaactgta tgggaaatat
    23221 aaggcgaaaa tcaatttttc tggaatcaat cgatcaaagg ataacgctga ggggcatttg
    23281 atcttagtta cttccatcaa tccaacacca gctggcgaag gaaagtcaac gatcactgta
    23341 gggttaggag acgcgctcaa taaaatcaat aaaaaatctg ttattgcatt gcgagaaccc
    23401 tcacttggtc ctgtgatggg aatcaaaggt ggtgctgcag gcggaggata tgcacaggta
    23461 ttgcctatgg aagatatcaa cttgcatttt actggtgaca tgcatgctat cacaacagct
    23521 aataatgcat tatctgctct tttagataat catattcatc aaggaaatga attgaacatc
    23581 gatgcccgcc gagtgatttg gaaacgggta gtcgatctca atgatcgtga actgcgtaaa
    23641 gtaattgttg gcttgggtgg gccaatccaa ggtgttccaa gagaagatgg gtttgatatc
    23701 acagttgcca gcgaaatcat ggctatcttg tgtcttgcgg cagaccttga agatttaaag
    23761 gctcgtcttg caaggatcgt tgtcggctat acatttgacc gtcagcctgt gactgctgga
    23821 gacttgaaag cagaaggcgc tctggcatta ctgctgaaag atgcaatcaa gccgaatctt
    23881 gtacaaacaa tttacggaac accagctttt gtccacgggg gaccgtttgc caatatcgct
    23941 catggctgca atagtatttt agctaccaag acagcattga gattagcaga ttacgttgtc
    24001 acagaggcag gatttggtgc agatcttggc ggagagaaat ttttagatat caaagtacct
    24061 aacttgaaaa aagcgccgga tgctgtggtg attgtcgcca ctgtccgtgc attgaaaatg
    24121 catggcggaa tgaaaaaaga tgaacttaaa aatgagaatc ttgatgcttt gaaagtaggt
    24181 tttgcaaatt taaagcgcca tatacgtaat atggaacaat atcagctacc tgtgatcgtc
    24241 gcaatcaatg aattcgtaac agatacagac aatgaactca cactgttgga gcacttgtgt
    24301 gaaaatcagg ggattcttgc caagagagcg agtgtatggg cagatggagc agaaggcggc
    24361 gttgatttag caaaagctgt agtacgattg attgatagaa aagaagctaa ttacaagcca
    24421 ttataccgcc tagaagaaac gatacaagaa aagacagaaa caatcgtcaa gaaaatttac
    24481 ggaggaaatg gtgttgtttt cagcaaaaaa gcgcaaaaac aaatagaaga gtttacgaag
    24541 aatggatggg acaaactgcc gatttgtatg gcaaaaacac agtattcatt ttctgatgat
    24601 ccttcactgc taggtgcacc ggaagatttc accatcacga tccgcgaaat catcccaaaa
    24661 ttaggggcag gatttttagt agccttgact ggagatgtta tgacgatgcc tggtttgcct
    24721 aaaaaacctg ctgcattgaa tatggacgtg acaaatgacg gagaagtgtt gggattgttt
    24781 taagcttgat tataatctac catcgaagga gctgtatttt gactacagct tcttttttta
    24841 gacgataggg aattaatttt gtttacacta ggatcattct ttttctttat tgtattttga
    24901 gctatactaa agcataatag gagggtatga tgaaaacgac atttataaga aaaaccggtt
    24961 tttacggtat gggtagctcg attcagctaa gaatcaacaa tgaaaaacgc agtgttctca
    25021 gtcatgaaca aaagataacg cttgatctgg aagtgccctt tacgatgcag gtcacctttt
    25081 attggctaaa aagccccgtt tatacagtga aagaagcaaa gcctgcttat gtgatcacaa
    25141 tgaattactt gattcttcaa atctatccgt ttttatttct agcaactggg ttgggggccg
    25201 ttttcctaca aaatttattt ttcagttttt ttgcagttat tgtgttgatt gcgttctttt
    25261 tctatataaa aaaccgggca tatttgatca aggaggaaaa ttatggcaaa ctctgatctt
    25321 tttttaatca gtttcaatcg aatcgaaaaa tggatgagag acgagatggg aaatgcacga
    25381 aatatgggat ttaccgaact tgtcagacgc ctagctcaaa gaaaacaatt aatgatcaga
    25441 aaatacgaag atgatctttt acagcttgct cagttaagga atgcgatcgt ccatgatcgg
    25501 atcgcagtcg attttgtcat tgcagagcca aatgattgga cagtcaagcg aatccagctg
    25561 atcgaacaag aactgattca gcctgaaacc gtgctgcctc gatttgcaaa acgtgtaaca
    25621 ggttttgaac aagatcttcc gttacttgaa ctgttaaaga tagtagcaga aaaaaggtat
    25681 tcacaatttc cgttatacaa taaaggaaaa tttgtagcat tgattacttt acgtaatatc
    25741 gggttttggc tagcaaaaga aagccaaaaa ggtccggttg atttaacaaa taaacgagcg
    25801 ttggatttga tcatccaaaa cggaaagtac acaaattatc attttgttcc agctaaaaca
    25861 catatttacg aggttgaagc gatgtttcgt gagcaaggga ctttggaagc cgtacttatc
    25921 actaaagatg ggaatcccga tggtaatctg ctcgggatcg tacgacctcg tgatatttat
    25981 aaacaaatag aaaaggattg attttttgtt aattatttat tggatattca gcgctttgct
    26041 agtaggagtg gatcagttgg tcaaatggtg gatcactgat aatctagcgc ttggagagac
    26101 gaaaaatctt ataccaggta ttttgtcact gaatcatatt cgaaacactg gagccgcatg
    26161 gagtatgcta gaagggaaga tgtggttctt tactatagtg acactcattg cagtcgttgt
    26221 gattctcaca ttgatgatta aaaatcgtga aaaaggtaat cgctggttca tgattggatt
    26281 gagtttgatt ctagctggtg cgattggcaa ttttatcgac cgtattcgtc ttggctatgt
    26341 agtagatatg ttccagacag attttatgag ctttccgatt tttaacgtag cagatgtgac
    26401 acttgtatgt ggagtgattt gtatgttaat ttatattatt ttagatgaaa aggatcaaag
    26461 aaaaaaatga cagaacttaa aacgacgatt caccatgaat ctggtaggat cgataaagta
    26521 ttgactaatt tattcaccga ttacagtcgt tcgcaaattc agctgtggct gaaaaatgga
    26581 gcggtttctg tcaatggaca gccagtcaaa gcaaattaca aagtaaaaca aggagacgag
    26641 attactgtct tgatccccga accagaaaca ttagatgtgg tcccagaaaa tatcccgttg
    26701 acgatcgttt acgaagatga agatgtagcg gtgataaaca aacctcaagg aatggtcgtt
    26761 catccttcag caggtcatac gcatggcaca atggtcaatg cgctgatgta ccatatcaag
    26821 gatctttcta cgatcaacgg cgttattcgt ccaggtattg ttcaccgaat cgacaaagat
    26881 acttccggac tgctaatggt tgccaaaaat gaccaggcac acgaatcatt agctcagcaa
    26941 ttaaaagata agacctcttt gcgcaaatat gttgctttag tccatggaga gattcctcac
    27001 gaaaaaggaa ccatcaatgc gccaatcggc cgttcgaagg aaaaccggaa aatgcaggcg
    27061 ataagagaag atgggaagtc ggctgtcaca cattttactg tattggaacg tttcgaagga
    27121 tttactctgg ttgaattaaa actagaaacc ggaagaacac accagatccg tgtgcatatg
    27181 aagtatatcg gttatccgtt agctggagat ccagtatacg gaccgaagaa aactttgaaa
    27241 ggcaatggtc agtttctcca tgcgcaaatg ttaggtttta cgcacccgac tacaaacgaa
    27301 aagatgatct ttgaagcacc tcttccagaa gtttttgaaa aaactttaga aaaattaaga
    27361 aaaaacattg cattttaatt tagataacgg tatagtaatt aacaacgaaa gcataaaact
    27421 taatagaatt cctttaagtt tagtcccgtg aggctaagaa gggtgggagc aaggatacgt
    27481 atggtgcgcc ataagtagat tgctcattga catagtatag ctatcagtgt atcagtatac
    27541 gctcagtcta aacattcctc ctgtcctctt agggcaggag tttttttatg caaaaaagta
    27601 gggggaatga aaatgcaagc aaaagaagta gtagatcagg taaccatgaa gcgagcattg
    27661 actcgaatta cttatgagat tatcgagaga aaccatagta tccaagatat cgtattggta
    27721 ggtatcaaaa cacgaggaat ctacattgca tcaagaattg ccgaacgcct gaagcagcta
    27781 gaagatatcg atattcctgt aggagaactg gatatcactt tataccgaga tgacaaaaaa
    27841 gaaaatccag aagaaccgga acttcattct tctgacatcc cagtttcctt agaaggaaaa
    27901 gaagttattt tgattgatga tgttttgtat acaggacgaa cgattcgagc agcaatggac
    27961 gcagtaatgg attttggcag accaagaaaa atttcattag cagtcctggt cgatcgtggg
    28021 catcgagaat tgccaatccg tgcagattat gttgggaaga acattccaac atctcgggcg
    28081 gaagagattt tagtagagat gcaagagctt gatggacaag atcgcatcat gattttgaaa
    28141 gaggaagatt agaggagaga gaaacatgtc ggagaaaaaa ggatttcata atgatgacgt
    28201 ggtgttagat atccacgatc gcccaacccc aggacattgg gtaggattaa gtttacaaca
    28261 cttgttcacg atgttcggag caaccgtgtt agtccccatc ttagtaggta tcgatccagg
    28321 tattgcgcta gtcagttcag gactaggtac attggtgtac ttatttacta ctaaaggaaa
    28381 gatacctgcg tacttaggaa gcagcttcgc tttcattgca gcgatgcagc ttttgatgaa
    28441 aacagatggt tatccagcga ttgcacaagg agcagtcaca actggattag tctatttgat
    28501 tgttgcgctg atcatcaaaa agatcggttc agcatggctg gataaaatct tgcctccaat
    28561 cgtcgtcggt ccagtcatca tggttatcgg gttgggactt gcggcaaatg cagcaacaaa
    28621 tgctatgtat aatcagggca cgtatgattt caagtatatt gctgtagcgc tgatcacatt
    28681 aggattaaca attttcttca acatgtggct aagaggtttt ctcggattga ttccgatttt
    28741 actaggtatt attagcggat acatcgtagc cttgctgttt ggaatcgtgg atactaagcc
    28801 aatcgtagac gctgcctggt ttgctgttcc taactttgag attccctttg tacaatatga
    28861 accaaagcta tatcttggag caatcacaac aatggcaccc atcgcttttg tgacgatgac
    28921 agagcatatc ggccatctta tggtattgaa taaactaact aaacgtaatt tctttgaaga
    28981 accgggtctt cataagacgt tgacaggtga tggattagca caaatcgtgg cagcctttgt
    29041 cggaggtcct cctgtaacaa gttacggtga aaacattggt gttctggcta tcacacgtgt
    29101 ccatagcgtg tttgtgatcg gtggagctgc agtatttgca gtcattttag gattcgtagg
    29161 aaaattaagc gctttgattt taagtatccc tggaccagtc atctcaggaa tcagttttat
    29221 cttatttgga gtaattgccg caagtggaat gaagatttta atcgagaaca aaatcgattt
    29281 tgataaaaag aaaaatctgt tgattgcttc agttatctta gtagtaggaa ttggcggact
    29341 cgtctttgaa gtaggcacat ttacattgtc ggcaatggca ttagcaactg tacttggcat
    29401 cattttaaat cttatcttac cagaaacttc acgaagcgaa gaaaactaag gaggaaacat
    29461 tatgatcatc aaatcagaac gtatcagctt aaagcattta ttgaccgttg aggcacttac
    29521 tgatcaagaa gttatgggct tgatccgcag agggcaggaa ttcaagcaag gtgctaattg
    29581 gactccgcaa aagcgccagt actttgctac aaacctcttt ttcgaaaata gtactcgcac
    29641 acataaaagc ttcgatgtag cggagaaaaa actagggcta gaagtgattg aatttgaagc
    29701 aagcacaagc tctgtccaaa aaggggaaac actatacgat acagtactga cgatgtcagc
    29761 attaggcgta gacgtagcgg ttatccgtca tggtgatgag aactattatg acgaactgat
    29821 acaaagtaaa acaatccagt gttcgattat caatggagga gacggaagcg gacagcatcc
    29881 aacccaatgc ttgcttgatt tgatgaccat ttatgaagaa tttggtcatt ttgaaggtct
    29941 gaaagttgcc atcgtaggtg atatcactca ctctcgcgta gctaaatcca atatgcagat
    30001 gttaaaacgt ctaggcgcac aagtattctt ctcaggtcct agagaatggt acgacgatga
    30061 attcgaagtg tacggacatt atatgccatt ggatgaacta ctagatcaag ttgatgtcat
    30121 gatgatgctt cgggtgcaac acgaacgcca tgatggaaaa gaaagtttct caaaagaagg
    30181 ctaccatcag gaacatgggt taacagttga gcgtgcgaag aagttgcaaa aacatgcaat
    30241 cattatgcac ccagcaccag ttaaccgtga tgtagaatta gctgattcat tagtggaagg
    30301 attacagtcg cggatcgttc agcaaatgag caatggagta ttcgtcagaa tggcaatctt
    30361 agaagcagtg ctaaatggaa aagcgtaatt ataggaggat gaaaaaatga aaacactcat
    30421 taaaaacggt cagatcaata cacgaaaaaa tatgacgaca ccagcagaaa tttggataga
    30481 agatgggcga atcaaagcaa tcggtacagg attctcagaa gccgaatttg atgaagtgtt
    30541 tgatgcaaag ggacaattga tcacacctgg tttagtggac gtccatgttc atttgagaga
    30601 gccaggattt acatacaagg agacgatcga agctgggact cgctcagctg ctcgtggcgg
    30661 tttcactact gtatgcgcga tgccgaattt aaatcctgta cctgatacgg cagaaaagct
    30721 gagacaagta tatgacatca ttagaaaaga tgcagttgtg aaggtattgc agtatgcgcc
    30781 aatcacagaa aatctgcgaa gtgagaaatt agttgatcaa gaagcactga ttgaagaagg
    30841 tgcttttgct ttcacaaatg acggtgtagg tgtccaaaca gctgggacga tgtatctggc
    30901 tatgaaagaa gctgcaaaaa acaataaagc cctagtagcc catacagaag atgaatcttt
    30961 attatttggc ggcgttatgc acgctggcaa gaaggcagaa gaacttggtc ttcccgggat
    31021 cttaagcgta accgagtctt ctcaaattgc tagagatctg ttgctagctg aagcaactgg
    31081 tgttcattac catgtttgcc atgtttctac taaagaaagt gtccgtgtaa tcagagacgc
    31141 aaaaaaagca ggaatccacg taacagcaga agtttcaccc catcatctta ttttgatcga
    31201 cgaagatatt cccgaagatt tcggcttttg gaaaatgaac ccgccgctaa gaggacggga
    31261 ggacagagaa gctttgatcg aaggcttgct tgacggtacg atcgattgca tcgctacaga
    31321 ccatgccccc catggattag aagaaaaaag ccaaagtttc atgaaatcac catttggtat
    31381 cgtaggaagc gaaacagcgt ttcaactgat ctatactcat tttgtagaaa ctggtcgatt
    31441 cacgttggaa caagtgatca attggttggc agttaaaccg gctgagattt ttggtttgaa
    31501 tgcaggaacg ctaacggttg gtgcccccgc tgatgtagca gtttttgata ttacacaaac
    31561 ttgcacgatc gataaagaag actttttatc aaaaggggag aatacacctt ttatcggctg
    31621 gaaagtaaaa ggcgaaacac aaatgacttt tgtcaacgga aaacttgtgt ggcagaaggg
    31681 agaataaaag tatggaacgt ttgcttatat tagaagatgg aacagttttc gaaggaaagg
    31741 cttttggtgc agatgctagt gtcttcggag aaatcgtttt tacaacaagt atgactggtt
    31801 atcaagaaac tatcactgat caaagtttta acggtcaaat cattacattt acctatccta
    31861 tggtgggtaa ttatggggta aatagagatg attatgaatc gatttctcca acatgcaaag
    31921 gagtagtagt aaaagaacat gcacgtgtag cttcaaactg gcgaaaccaa atgacgctag
    31981 attcattttt gaaacaaaaa ggaattcctg gaatatcagg tatcgatacg cgcgccctta
    32041 ctcgcaaaat ccgtgaagtc ggaacgatga aagctagtat agttgatgct ggagattcct
    32101 tcgaacatgc atttgatcag ctgaaagcaa ccgttctagc aaccaatcaa gtgcagcaag
    32161 tctcaactaa caaaccttat ccaagtccag gaacaggtcg gaaagtcatt gtcattgact
    32221 tcggcttgaa acatagtatt ttacgagaat tgagtaaaag aaactgtaac ctcacagtac
    32281 ttccctataa cacagatgcc gagacgattt tagcgctatc accagacgga gtcatgctga
    32341 cgaatggacc tggtgatcca aaagatgtgc cagaagcaat cgaaatgatc aaaaaaatcc
    32401 aaggaaaagt accgattttt ggtatttgtc tcggtcacca gttatttgct ttagccaacg
    32461 gtgcagatac ctataaaatg aaattcggac atcgaggatt aaatcacccg gtacgagaag
    32521 tagcgaccgg aagaattgat tttacttctc aaaatcatgg ctatgcagtc gatgaagcca
    32581 cgattgatcc agaaaaactc atcgtaactc atgtagaagt aaatgacgga acagtagaag
    32641 gagtcaaaca cagagattat cccgctttca gtgtgcagtt tcatccagat gctgcccctg
    32701 gtccgcatga tgcactacat ttatttgatg aatttatgga aatgatggac gcaggaaagg
    32761 agcagtaaac agatgccgaa acgtacagat atcaagaaaa ttatggtgat tggatctggt
    32821 ccgatcatca ttggtcaagc cgcagaattt gactacgcag gaacacaagc ctgccttgcc
    32881 ttaaaagaag aaggatatga agtggtttta gtaaattcta atcctgcgac gatcatgacg
    32941 gataaagaag tggcagatca agtatatatc gaaccgatca cacttgaatt tgtatcaagg
    33001 attcttcgaa aagagcgtcc agatgctctc cttccaactt taggcggaca gacaggcctc
    33061 aatatggcaa tggagctggc agcatcaggt attttagaag aattgaacat agaacttcta
    33121 ggaacaaagc tttctgcgat cgatcaagca gaagatcgtg atctattcaa acagttgatg
    33181 aaagaattga atcagccaat ccccgagtct gaaatcgtca atacagtaga agaagctgta
    33241 gattttgcaa ataaaatcgg ctatcctgtc atcgttcgtc cagcctttac tttaggcgga
    33301 actggtggag gtatgtgtga aaatgaagaa gaattacgta tcgtatcaga aaatggatta
    33361 agtctttccc ccgctaccca atgtttgatt gaacgaagta ttgcaggttt taaagaaatt
    33421 gaatatgaag tgatgagaga ttcggctgat aatgcaatcg ttgtctgcaa tatggaaaat
    33481 tttgacccgg tgggtatcca tacaggtgat tctatcgtat ttgcccctag tcaaacattg
    33541 tctgaccatg aatatcaaat gcttcgtgat gcttctcttt cgattattcg agcacttaag
    33601 atcgagggag gctgtaatgt ccaactcgct cttgatccac atagttttaa ttattatgtg
    33661 attgaagtca atccgcgtgt ttctcgttca tctgcgttag caagtaaggc gactggctat
    33721 ccgatcgcta agctagctgc taaaatagcg cttggattaa cattagacga aatgaaaaat
    33781 cctgttacag gaacgacttt tgcagaattt gaacctgcat tagattatgt tgtagctaaa
    33841 attcctcgct ggccgtttga taagtttgaa aacggagaac ggcttttagg cacgcagatg
    33901 aaagcaactg gtgaagtgat ggctatcggt cggaatatcg aagagtcttt attgaaagct
    33961 gtgcgttctt tagaaatcgg tacacaccat atcgaacttc ctgagttggc agacatcacg
    34021 gacgatgacc taatccaaaa agtggttcga gcacaagatg atcgcctgtt ttatgtagct
    34081 gaagctatta gaagagggta caccatagaa gaattagcgg atttgacaaa aatcgattta
    34141 ttctttttag ataaactgtt gcatatcgta gaaatcgaac agcagttgaa acagcagccg
    34201 ttggatgaca agatattgaa aacagcgaaa caaaacggtt ttacagacaa aaaagtggcg
    34261 gagctgtggg aaataaacga agaagccgtt cgccagatga gaaaagaaaa tgatttgatt
    34321 cctgtatata agatggttga cacttgtgca gcggaattcg aatcaaaaac accttatttt
    34381 tatagttcct atgaatatga aaatgaaagt tcacgatcag aaaagccctc aattctagta
    34441 ctgggatctg gtcctatccg tatcggacaa ggggttgaat ttgactatgc gactgtgcat
    34501 tctgtcaaag caatccagca ggcaggatac gaagcaatca tcatgaacag caatcctgaa
    34561 acagtttcga ctgacttttc tatttcggat aaattatatt ttgagccatt gaccttcgaa
    34621 gatgtgatga atgtgatcga gctagaacag ccgataggag taatcgttca atttggtggt
    34681 cagacagcaa tcaacttagc cgaacctttg actgctgctg gtgtaaaaat cttagggaca
    34741 acgatcgaag acttggatcg tgcggaaaat cgtgatttat tcgagcaagc gctgaaagag
    34801 ttggatattc ctcaaccttt aggggatact gctacaagta aagaagaagc cgtggttatc
    34861 gcttcgagaa ttggttatcc agttcttgtt cgtccaagct atgtattagg cggacgtgcg
    34921 atggaaatcg ttgaaaacca gcgagattta gaagattaca tggaacatgc tgtgaaagca
    34981 tctccagaac atccagtact agtggaccgt tacttgcttg gtggtgaatg cgaagtggat
    35041 gcgatttgtg acggtgaaac agtactcatc ccagggatca tggagcatat tgagcgtgct
    35101 ggtgtccatt caggggattc aatggctgtg tatccacctc aaacattatc tgaatcgatc
    35161 aagaaaacga tcgaagatta cacgatccgc ttagcattag gtttgaattg tgtcgggatg
    35221 atgaatatcc agtttgtcat ccatgaaaat gaggtttatg tgatcgaagt caatcctcga
    35281 gcaagtcgga ctgttccatt cttaagtaag gtgacaggaa ttccaatggc acaaattgcg
    35341 acgaaagcta ttttgggaga gaaactaaag acacttggtt atgaaaacgg actttatcca
    35401 gaaacaaaag aagttcatgt taaagcacct gtcttttctt ttacgaaatt gcagaaagtt
    35461 gatacttact tagggcctga aatgaaatcg actggagaag taatgggatc tgacagacaa
    35521 ttagacaaag ctttgtacaa ggcattcgaa gcttctggat tacacttgcc tgaatatgga
    35581 gccgtattat ttacgattgc ggacgaaaca aaagaagaag cactagaatt agctaaacgt
    35641 tttacagaaa ttggctatag tctgatagga acgaaacata cagctgctta tttcgaaaaa
    35701 aatggtctgg tcgttgaaac agtagcgaaa atttctgagg aagcagcaga aaaaaatgta
    35761 gtcgacttaa taagagatgg taaaacacaa gtggttgtca atacgattga taaagatcgg
    35821 ggaaatgctt ctaaagacgg tttcatcatc cgaagagaag cagttgaaca tggtattcca
    35881 ttgtttactt ctttagacac agcagatgcg atcgttcgag tgatggaatc ccgtgctttt
    35941 tcaactaacc ctatttaacg gcgagcacat gttaagacaa aaagacgaaa aaccgttgca
    36001 ggaggggaca gatgaaacaa gaaatgatgg aaatcgttca gcaaaaacag ctggcaccaa
    36061 agatttttga aatgacattg aaaggtgatc tagtaaaaga aatgcagcaa ccaggacagt
    36121 tcttgcatat tctcgtgccg cgtgcagact tgctattacg ccgaccgatt agcttgaatc
    36181 atatcgacca cgaaacaaat acttgccgta tcatttatcg ggtggaaggt gaggggacta
    36241 gagtcttctc tactctttct gccggagacc atttagatgt catgggtcct ttaggcaatg
    36301 gttttgattt gtccaatctt catacaggag acgaagtatt tataatcggt ggaggaatcg
    36361 gtgtccctcc tctatatgag ttatcaaaac agttagtcga aaaaggaatt aagccaacgc
    36421 attttttagg ttttgccaca catgaagtcg tttattatga aaaagaattt aatcgtctag
    36481 gagaaacaag aatcgctact gacgatggaa catacggtat gcacggaaat gtcggtaatt
    36541 tattgttagg aacgaagacg gaacctacag ctgtttttgc atgtggttca aacgggttat
    36601 tgaagacagt ggaacagctg ttttcttcgc atcaaaatgt ccagttatca ttagaatccc
    36661 gcatggcatg tgggataggg gcttgctatg cttgcgtttg tcatacgccg gaagatgaaa
    36721 caggaacaaa aagtgtgaaa gtttgtgacg aaggaccgat ttttaaaata ggagaggtaa
    36781 ttatatgaat gagaatcctt tagcggtcaa acttcctggc ctagaattaa aaaatcctat
    36841 catgccggca agcggctgtt tcggttttgg caaagaatac ggaaaatact atgatttgaa
    36901 tcagcttgga tcgatcatgg tcaaggcaac gactccaaat gcccgctttg gcaatcccac
    36961 gccaagagtg gcggaaacac catctggcat gttgaatgca attggtttgc aaaatcctgg
    37021 tctagacgtt gttatgcata aacttcttcc agaattagag gagtacaatg agttacccat
    37081 catcgcaaat gttgctggtg cttgtgaaga agactatgta gaagtttgta gcaaaattgg
    37141 cgaagcacca aatgtgaaag caatcgagct gaacatttct tgcccaaatg tcaaacatgg
    37201 cggaattgct tttggaacag attctgatgt tgcttttcaa ttaacacaag cagtaaagaa
    37261 agtttcttct gttcctgtct atgtcaaatt gtccccaaat gtaacagaca tcgttccaat
    37321 cgctcaggca atcgaagctg gaggggcaga tgggtttacg atgatcaata ctcttcttgg
    37381 tatgcggatt gaccttaaga caagaaaacc gattttagcc aatcaaacag gtggactctc
    37441 cggaccagca atcaagcctg tcgctattcg tttgattcac caagtagcaa gtatatccag
    37501 ccttccaatc attgggatgg gcggcgtgca gacagtggat gatgttcttg agatgtttat
    37561 ggctggagca agtgctgtag ctatagggac cgcgaatttc accgatccgt atatctgtcc
    37621 taaactaatt aaagagctcc ctgtgcgaat gagcgaacta ggcatcgaat cgctggaaag
    37681 attaatcaaa gaagtgagag aggaacgtga acgatgaatc aactagacca acgaccaatt
    37741 attgcattag atttttcaac taggcaagag gtagaagact ttcttcgatt tttcccgaaa
    37801 gaagaaaaat tgtttgtgaa aatcggaatg gaattgttct accaagaagg acctgaaatc
    37861 gtacgttatc taaaagaggc tggtcataat gtatttttag atctgaaact tcacgatatt
    37921 ccgaatacgg tcgaaaaagc aatgcgggga ttggcaaagc ttggtgttga tctcacctgt
    37981 gttcatgccg ctggcggtat tcgtatgatg gaggcggcaa tgcgaggatt agaagagggg
    38041 actccagaag gtggaaaacg tccgctactt ctagctatca ctcagcttac ttctacaagt
    38101 gaagaagaaa tgcatgccga tcaactaatc gaagtttctt tagaaaaaag tgtgatccat
    38161 tatgctagct gtgccaaaaa agcaggatta gatggtgtgg tttcttctgc ttgggaagta
    38221 gaagcgatca aagaaatggc aggagacgaa tttgtttgct taactccggg tataagacca
    38281 gaaggaacag tcgcaggaga tcagacaaga gtagtcacac cttctcaggc aaaaaagatc
    38341 ggctcaactt ttatcgttat aggtcgacca atcactcagt caactaatcc ttataaagca
    38401 tatcaaacga ttcaaacaga atggagctaa cctaaaatga atgttgaaca aagtatcgca
    38461 aaagacttat tagaaattga agcagtattt ttaaatccaa gtaatccttt tacatgggca
    38521 agtggaatca aaagcccaat ttactgtgat aaccgaatca cgatgagcta tccaaaagtc
    38581 cgaaaagaga tcgcaaaagg attagcaagc aaaatcaaag aagcttaccc tgaagtacaa
    38641 gtgattgctg gaactgcaac agcaggaatc cctcatgccg catgggtagc ggaaatactg
    38701 gatttgccaa tggtctatat tcgaagtaag gcgaaagatc atgggaaagg taaccaaatc
    38761 gaaggacgta tatttgaagg tcagaagatg gttgtgatcg aggatttgat ttctacaggc
    38821 ggaagtgtct tggaagcagc tgaagcagca aaaagagaag gtgcggatat ccttggtgtt
    38881 gcggctatct ttacgtatga gttgccaaaa ggaaaagcaa atttcgaaaa agccgaaatc
    38941 cctcttatga cgttgaccaa ttattctgta ttgattgaag cagctttaga agatcgttat
    39001 atcgatgagc aagaattaac tctgctaaaa gaatggaaaa aagatcctga aaattggcag
    39061 attgaagcgc gcttgatgaa gatagagaaa tccgatttgt gtctccagaa tcatcaattg
    39121 gaatagcaaa aatccaaaaa ctgattgcga gaagcatctt ttcatcaggt atgaaaagaa
    39181 acttctatcg tgatcagttt ttgatttttt taaaattcat ttttctaaga ctggttagca
    39241 gaaagaagag aataatctct tctctttttt ctattatata tagattttct ctattttttg
    39301 gtaagggagc tgtttcaaaa atggtaaaga gcggaaatgt tgcgaatatc agttatttaa
    39361 tgatatgata ttcggaaaaa gaaatctcaa agggaggtaa cttttatggg gttgaatgaa
    39421 tttattttag gattgaacaa tttagagacg atcacaaggg cacctggatt ttttaaatat
    39481 acagagcata cagttgcagc acattcgtat cgtgtagcat ctattgcgca agttctagga
    39541 gacattgaag aagcgaatgg acaaccgatc gattggaaac tgctctatga aaaatcattg
    39601 aatcatgact acacagagcg ttttattggt gatatcaaaa cgccggtgaa atacgcaagc
    39661 aaagagttaa gagggatgct gcaaacagtt gaagaaaaga tgacggacga attcatcaac
    39721 caagagattc cagctgagtt ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat
    39781 gaaaccgtag aaggagaact attagccatt tcagataagg ttgaccttct ttatgaatct
    39841 tttgatgaaa tcatcaaaaa taacccggaa aaagtttata aggagatgtt cattgaggca
    39901 gtgactacga ttaaaagctt tgaacatcgg ccttctgttc agtatttctt taaagagata
    39961 tttccggaac tattgaacca ggaattttat ggaaaagagg cgtttttaga aaccgtctca
    40021 acgattgtaa agaaagaaga aaaataaaaa tgatatgaga tgcaggggga agtatagttg
    40081 aaaaagaata tggatgtcga atggggctat tctggagaca aaggtccgga aaattgggcg
    40141 agtttatgtg attggtttgc gagaggtgcg gaatttccat tgcagtcgcc tattcatttg
    40201 accaaggggg aagaaaccaa aatagaagga gacacgcttt cctttcatta tcaaaagcaa
    40261 cggtttactg acaaggaatt caaaaacacg attcatttag taccttttga ccaactcagt
    40321 tatgtagagt tcaagaaaga acgctattat ttgacagata ttcatttcca cttgccttct
    40381 gaacatatca tcaacggaaa acaggaagat attgaatttc attttgttca tatgaacagc
    40441 aaaaaagaga atctagttgt gggtgtcatg taccagctaa tggtacagga aggttggctg
    40501 tataatcaag aaaatgggga aagttggaac ttagaaaaac atgagcattg ggtaaatccg
    40561 gatgtgttct ttccggagca aaaaagccat ttccattata ttggctcatt gacaactccg
    40621 cctaccagcg ggccaatcca atggtttgta atggatcatg taggaaaact gaaccaagag
    40681 tttcttctcc ccttcgatgg tcagtatgct cgaccgaaca accgaccaat ccagcccttg
    40741 aatggaagag aaatttatta tttcgaggag tttgagcaat gaatattcag cagatgaaat
    40801 acgtggtcgc tgtagcaaac aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct
    40861 cccagccaag tctttcagct ggtatcaaag aattagaaac cgagttaggg atcacgctgt
    40921 ttactcgaac gaatcgaggg gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg
    40981 ccgagaaaat attggtccag cttgatacat tagaaactca ctatctaacg agtgaaaaga
    41041 gagacaattt ttcgatcgct gcccagcatt atgatttctt aggtcctttg accgcacagc
    41101 tactcgagga attcaaagac gagatcaaac aatttcggat catggagacg acaacagcga
    41161 gagtgatcga agaagtcaaa gagcagcaca gtgagatcgg cattatttat atgaatgagc
    41221 ataatcaagc tggtatcaga agatatttac aacaaggaga gctaagtggc cgcagcattg
    41281 gaaaattcaa aacgcatatt tttttaaggc aaggacatcc attagctgaa cacgcagaga
    41341 tcaccaaaga agaattgtta tcttatcctc aagttcgttt tacacaggat ggaaataatt
    41401 tcccgtattt ttatgaagac cttatcgaga tccctgatca agaaacagtg atttatacga
    41461 gtgatcgagg tactttgatg aatatcgttc ttgaaactga tgcttacgct tcaggatctg
    41521 gtattgtgat tggagggatt cgcgagcact tgcggttaat ccctttggca gaaggcgaac
    41581 taaatgaatt ttatatcatt tattcggcga aacgtccgct cagtgaaatc gctcaaagat
    41641 ttatcaaaga gcttactcgg ttactggacc agcagagcta agaaaagcag atgagaacgc
    41701 tgtttattga acaccgttga tcaaaaaaga ggggttgtga cagaattttg tcacaactcc
    41761 tctttttgta gcaaacaatt tataactaat tgctgttgtg ggaaagtacg caaatatgat
    41821 tctagaaaca ctcattgatt acctgttttg tttcatagct gtaatttttt cttcttctgg
    41881 tgtcacgata atcgtctttt ggttttcata aataacataa ccaggcttgg cgccatttgg
    41941 ttttcggata tgctttacct gtacaagatc aaccggcact tgggcagagt atcggtattt
    42001 tgaaaaatag gcagctaact cggcagcttc tgtaattgtt tcatctgagg gcttgtcact
    42061 tttaatgatg acgtgcgaac cgggtatatt ttttgcatga agccaatagt cggttttttt
    42121 agctgtcttc attgtcagct ggtcattttg aagattgttt ttaccaacaa gaatgagcgt
    42181 tccgtcagaa gaaaagtatt gatcaggctg agatggtttc ttacgttttt gtttttttaa
    42241 gttcttcttt ttcaggtatc cttctgaagt caattcttct ttaattgctt cgatatccat
    42301 tgggccggca atttccagtt gagataagac agattcaaga tattggattt catctttggc
    42361 ttcttggatt tgttcgccaa tcagcttaac agcgttcttc agcttttggt atcgatggaa
    42421 atatttctgt gcattttgat ttggtgtcaa tgccggatct agtgctattt tgattggccg
    42481 atcttcttca taataattag gtaaaacaac ttcttttgcg ccgcgtggta cttgtgtcag
    42541 aaaagtagta agtagttcgc catttctacg gtaattctca gcgttttcgg attctttgag
    42601 tgtttgttca cgttttttca atttattttt atttcgtttt agttcatttt cgatttttcg
    42661 aatcagttct ccgccttgtt gttttgcccg atctttctct gctttttcgt gataatagct
    42721 atctaataat tgactgagtg ttggataagc tgttacagca gatgcctgtt cggacaatac
    42781 ctgataagca atcggtgtga aaaaatcttt attttccgtt tcgtaaaaag taggaatagg
    42841 ctgatggctg atggctgaga aaaattcgtc ccatacaacc atcttttcat taggacgttc
    42901 cagtaatctt ttggtcagct cttgagcgat atcgaaaccg attccctgaa actgacgttg
    42961 gattgctttt ggatctagtt cagtttggga aagccgctga aagatttttt ctttctcgga
    43021 gctaaatggg ttcaagacct cttgttttgg cggagcaaca tattctacac caggaagcaa
    43081 agaacgataa gtgttttgag agcttccaat gtgtttgatc gcatccagta tttttccagt
    43141 ttctcgatta actaagacga tcgtactatg acgtcccatt aattcaacga tcaatatgat
    43201 attttgtaga tcacccaact cgtttcgttt agcaaagtgg aaatggatca ctcgatcatt
    43261 ttcgatttgt tcgatgtctt ctaaaatagc gccatccaaa tattttcgaa gcatcatcac
    43321 gaagttaggc ggattatcag gattctgata atcgatttgg gtaatctgta cacgagcata
    43381 acttgggtgc gcagataata ataatcgatg gtttttgccg cgtgaacgaa tcaccagcac
    43441 gatttcattt tcataaggct gatggatttt cgagatccgg ccagatagta acgtttcacg
    43501 caattcgttg accatcgcgt gggtaaatac accatcaaag gacatggttc attcctcatt
    43561 tcttttcata agtatattca ttataacgaa aaaaagaaga aaaaccaatt tggccgaata
    43621 gtaaagcaaa agtctagaaa aatcttggac taattacata aaaaacagtg tttctatcgt
    43681 tgaaatccaa atttttaggt a
//
LOCUS       DANKAZ010000015        41211 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000015 DANKAZ010000000
VERSION     DANKAZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            80..1084
                     /locus_tag="PWY67_000754"
     CDS             80..1084
                     /locus_tag="PWY67_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2057061.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1085..2029
                     /locus_tag="PWY67_000755"
     CDS             1085..2029
                     /locus_tag="PWY67_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2057062.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2029..2991
                     /locus_tag="PWY67_000756"
     CDS             2029..2991
                     /locus_tag="PWY67_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057063.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3018..3932
                     /locus_tag="PWY67_000757"
     CDS             3018..3932
                     /locus_tag="PWY67_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057064.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3958..5739
                     /locus_tag="PWY67_000758"
     CDS             3958..5739
                     /locus_tag="PWY67_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2057065.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6087..6773
                     /locus_tag="PWY67_000759"
     CDS             6087..6773
                     /locus_tag="PWY67_000759"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL2057066.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6793..10374
                     /gene="smc"
                     /locus_tag="PWY67_000760"
     CDS             6793..10374
                     /gene="smc"
                     /locus_tag="PWY67_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL2057067.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10383..11192
                     /locus_tag="PWY67_000761"
     CDS             10383..11192
                     /locus_tag="PWY67_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2057068.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11203..12201
                     /gene="ftsY"
                     /locus_tag="PWY67_000762"
     CDS             11203..12201
                     /gene="ftsY"
                     /locus_tag="PWY67_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL2057069.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12388..14655
                     /gene="gshAB"
                     /locus_tag="PWY67_000763"
     CDS             12388..14655
                     /gene="gshAB"
                     /locus_tag="PWY67_000763"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL2057070.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14744..15196)
                     /locus_tag="PWY67_000764"
     CDS             complement(14744..15196)
                     /locus_tag="PWY67_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL2057071.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15346..16296
                     /locus_tag="PWY67_000765"
     CDS             15346..16296
                     /locus_tag="PWY67_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2057072.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16289..17248
                     /locus_tag="PWY67_000766"
     CDS             16289..17248
                     /locus_tag="PWY67_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2057073.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17245..18000
                     /locus_tag="PWY67_000767"
     CDS             17245..18000
                     /locus_tag="PWY67_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2057074.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18023..18976
                     /locus_tag="PWY67_000768"
     CDS             18023..18976
                     /locus_tag="PWY67_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2057075.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDITKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19620..19916)
                     /locus_tag="PWY67_000769"
     CDS             complement(19620..19916)
                     /locus_tag="PWY67_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDL2057076.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20271..20627
                     /locus_tag="PWY67_000770"
     CDS             20271..20627
                     /locus_tag="PWY67_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2057077.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20637..21536
                     /locus_tag="PWY67_000771"
     CDS             20637..21536
                     /locus_tag="PWY67_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL2057078.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(21933..23012)
                     /locus_tag="PWY67_000772"
     CDS             complement(21933..23012)
                     /locus_tag="PWY67_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2057079.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            23248..23838
                     /locus_tag="PWY67_000773"
     CDS             23248..23838
                     /locus_tag="PWY67_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2057080.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(23966..25315)
                     /locus_tag="PWY67_000774"
     CDS             complement(23966..25315)
                     /locus_tag="PWY67_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2057081.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(25483..26223)
                     /locus_tag="PWY67_000775"
     CDS             complement(25483..26223)
                     /locus_tag="PWY67_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2057082.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(26236..27828)
                     /locus_tag="PWY67_000776"
     CDS             complement(26236..27828)
                     /locus_tag="PWY67_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2057083.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(28396..28692)
                     /locus_tag="PWY67_000777"
     CDS             complement(28396..28692)
                     /locus_tag="PWY67_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057084.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            28868..29593
                     /locus_tag="PWY67_000778"
     CDS             28868..29593
                     /locus_tag="PWY67_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL2057085.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            29586..30428
                     /locus_tag="PWY67_000779"
     CDS             29586..30428
                     /locus_tag="PWY67_000779"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL2057086.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            30431..30952
                     /locus_tag="PWY67_000780"
     CDS             30431..30952
                     /locus_tag="PWY67_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL2057087.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(31205..31768)
                     /locus_tag="PWY67_000781"
     CDS             complement(31205..31768)
                     /locus_tag="PWY67_000781"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2057088.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            32019..32288
                     /gene="rpsO"
                     /locus_tag="PWY67_000782"
     CDS             32019..32288
                     /gene="rpsO"
                     /locus_tag="PWY67_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL2057089.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            32476..34602
                     /gene="pnp"
                     /locus_tag="PWY67_000783"
     CDS             32476..34602
                     /gene="pnp"
                     /locus_tag="PWY67_000783"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL2057090.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            35112..35378
                     /locus_tag="PWY67_000784"
     CDS             35112..35378
                     /locus_tag="PWY67_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2057091.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            35555..37513
                     /locus_tag="PWY67_000785"
     CDS             35555..37513
                     /locus_tag="PWY67_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL2057092.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            37867..39168
                     /locus_tag="PWY67_000786"
     CDS             37867..39168
                     /locus_tag="PWY67_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2057093.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(39298..40668)
                     /locus_tag="PWY67_000787"
     CDS             complement(39298..40668)
                     /locus_tag="PWY67_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL2057094.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(40850..41173)
                     /locus_tag="PWY67_000788"
     CDS             complement(40850..41173)
                     /locus_tag="PWY67_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2057095.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
BASE COUNT        13169 a         7704 c         8684 g        11654 t
ORIGIN      
        1 ttataaaacg atttattctg tgtaatgtcc ttttttatta agaaaaactt ccatttagga
       61 agcagagagg ggtattcgat tgtccgataa ccaattatta gaagttcaga acctacatac
      121 aggattccga ctcaaagatg actattacga tgcagtagat gacgtttcat ttactttaga
      181 caaaaacgag attttagcaa tcgttgggga atctgggtgc gggaaaagta ccttggctac
      241 tacgattatg ggattacttg atcctaataa cacaaaaatt acaggtgaga ttttatataa
      301 cgatctgaat ttaatcgacc tgaacgaaac gctctataat aaaatccgcg gaaatgatat
      361 cggaatgatt ttccaagatc cgttgtctgc attaaatcct ctcatgagaa tcgaagacca
      421 gatcaaagaa ggactttcct atcacacaaa gatgaatgca gaacagcgtc aagctcgtgc
      481 actagaattg ctagaacaag tagggattcc taatcctgcg agagttggac gccaatatcc
      541 ccatgaacta tcaggcggta tgagacaacg tgtcatcatc gcaatcgcaa tcgcctgcaa
      601 acctccaatc gtcattgcag atgaaccgac gacagcccta gatgtaacca tccaagcaca
      661 gattcttgat ttactgaaag accttcagga agaaacaaaa acagggatca tcttgatcac
      721 tcatgactta ggagtcgtag cagaaatggc tgatcgagtg gcagtgatgt atggcggaca
      781 gttcgttgaa gtagcaggtg taaaagaact gtttgatcat cctaagcatc cttatacacg
      841 ctctttactg aactctatac cacaagaagg aaatcatgat gcagaactcc atgtgatcga
      901 aggtgtcgtt ccttctctga aaaacatgcc tcgtaaaggt tgtcggtttg caccacgtat
      961 tccatggata ccagcaagcg atcatgaaga agagcctatc cttcatgaag tcgcaccgaa
     1021 ccacttggta cgttgctcct gctataagca tttccacttt agagacgaaa aaggagaggt
     1081 gtagatgaca gaattaatca caatcaaaga cctgaaagtt cattacccta ttcgcagtgg
     1141 attcttcaat cgagtaacgg atcatgtata tgctgtagat ggtgtggatt ttattatcga
     1201 aaaaggaaag acatatggtt tagtgggaga gtctggctct ggaaaatcta ctacgggaaa
     1261 agccgttgtg ggattagaaa aggtaacagc cggacaaatc atgtatgaag gaaaagatgt
     1321 aacgaagcga agcaaccgca aaaaaatggg ttataacaag gatgtgcaga tgatcttcca
     1381 agattcaatg tccagtttga atcctaaaaa gcgagtgttg gatatcattg ctgaacccat
     1441 ccgaaatttt gaacggttaa gcgatcaaga agaaaagaaa aaggtaaaag gactgctaga
     1501 tatcgttggg atgccggaag atgcgctata taaatatcct catgaatttt caggcggaca
     1561 acgtcaacgt ctaggagtag cccgtgctgt tgctaccaat ccaaaactga tcgtagccga
     1621 tgaacctgta tctgctctgg atctatctgt tcaagcgcaa gtattgaatt tcatgaaacg
     1681 aatccagcaa gaatacggcc tcagctattt attcatttca catgatttag gtgtagtaaa
     1741 acacatgtgc gacaatatcg caattatgta caaaggtcga tttgtagaaa tcggtacaag
     1801 agaagacatt tataatgacc cgcgacatat ctatacgaaa cgtctgcttt cagctattcc
     1861 gcaaatcgat gtagaaaata gggaaagcca caaagaaaat cgacgaagag tagaacaaga
     1921 gtatatccaa aaccaaaaag aatactatga tgcgactggt cgagtctacg atttgcgtac
     1981 gatcacaact acacacaaag tggcgttgaa agatggaggt gcaagctaat gtggaaaacg
     2041 attctgcgac gtgttttatt gatgatcccg caagttatta ttttaagtgt tctgatcttc
     2101 ttattagccc aagcgatgcc gggggatcca tttaccggtt tgatcaatcc aaatcaggat
     2161 ccggcagtga tcgaacagat gcgacaagca gctggcttga atgacccatg gtatgtccag
     2221 tacttccgtt ggatcggaaa tgctttgcaa ggcgattttg gtcaaagttt cttatacaaa
     2281 cgctctgttg ctgacttgat cggcgaacgg atcatcaaca ctttatatct atcgatcttg
     2341 acagttgtcc ttacttattt gattgctgtg cctctgggga tgcttgctgg ccggtatcaa
     2401 aattcaatct tggataagat tgtcgtaatc tacaacttct ttagttttgc tgttccttta
     2461 tttatcttcg gcttgatcat gctatttgtt tttggttatc gtttaggctg gttcccaaca
     2521 agtggatcac aaacactagg tttgcaaggt ggatttatca ctcaatggat agaccgtttg
     2581 aaatatattc tgttaccatc aattacccaa gcatttttgg cgactgccgt cacaatccaa
     2641 tatctgcgaa gtgaagtaat cgattcgaaa tcgctcgatt ttgtcagaac agcccgttcc
     2701 aaaggggtac cgacgaataa agtattcagt cgacatattt tccgaaatgc agcgttgccg
     2761 atggcttctc aaatggggta tgaaatcact tcgttgattg ctggttctgt agttatcgaa
     2821 aagatttttg gttatccggg agtcggaaaa ctatttattg attccatcgg ccaacgggac
     2881 tataccgtga tcacatcctt agtattgatt ttaggaatcg cgacgctagt ggggacatta
     2941 ctttcagata tcatcatgag tatcgttgac ccgcgtattc ggattcaata aaaccaatca
     3001 gaaaggagaa tggatcgatg agtgaaaaaa acaatacagc agttcaagaa agtattcctc
     3061 ctatggggat acgaatgatt gctcgagaat ttaaaaaaga taaaattgct attttttcat
     3121 taggtttgct agtggtttta ttattagtga tctttatcgg tgcgatgctg accgatcaag
     3181 acaaagtgat gtatgttagt atcttcgaca aatacgctgc accaggaaca gtttcgacac
     3241 aaggacaaaa gttcattctg ggagcagatg aaggcggacg tgatgtatta ggacaattga
     3301 tcataggtgc tagaaactct gttacgatcg gatttgcgat cacgatcctt acttcgatca
     3361 tcggggttgg attagggatc atctctggct actacggagg gatcattgac aacatcttga
     3421 tgcggatcgt tgattttatc atgattctac cgatcatgct gatcattatc gtcttcgtat
     3481 cgattatttc cagatacaat gtctggtcct ttatattcat catgagtgca ttttattggg
     3541 tagctaaagc ccgattattc cgaagcaaga cattatctga agcaagaaga gattatgtca
     3601 gtgcctcaaa aacaatggga accaatgatt ttaaaatcat gttccgcgaa atcatgccga
     3661 atctaagttc attaatcatt actaatttga cgatgaactt tgcggcaaac atcggtatcg
     3721 agacaacttt gactttctta ggtttcggct tgccggcaaa tgtacctagt ttaggtacat
     3781 tgatcggtta tgcaagtaac ggagaagtct tagtgaatcg gcaatggatc tggttacccg
     3841 catcaatcct tattttagtt ttgatgttga gtatcaatta tgttggacaa gcattcaagc
     3901 gttctgcaga tgcgcgccaa cggttaggct agtatagaag cgaaggggga aataggaatg
     3961 aagaaaacga tttttggatt tgcagcgctg atgtctgctg tcgttcttgc agcatgcggg
     4021 ggcggaggta cagatacctc tggaaacaac tctggaggca acggcaataa caaagaggaa
     4081 ttaacttttc caattgcaac aaaaaataat gatgatgcaa tcgaagacgg cgaattagaa
     4141 gctgcggtcg caacggacag tcagtttaaa ggattgttcc agtgggagtt ttaccaagat
     4201 tcatatgatg cagcttttat ggctccttct catgaaccgc tatttgcaaa cgatgacgat
     4261 ttcagaatca cagacgacgg agctgcaacg cttgatctag atgaagatac gaaaaaagcg
     4321 acaatcaaag taaaagacaa cgtcaaatgg tcagatggtg aagatgtaac ggcggacgat
     4381 gtgattttcc cttacgaaat cattgggaat aaagattaca ctggtatccg ttatgacgac
     4441 actttccgta acatcgtagg tatggaagaa tatcatgatg gaagtgccga cacaatctcc
     4501 ggaatcaaaa aaatagacga caaaacagtt gaaatcgaat ataaagaagt aaacccaagc
     4561 atgcttcaat cgggtggtgg tatctggtca tatgcagcac caaaacatac gttagaaggt
     4621 gtagcaatca aagatatgga atcaagcgat caagtacgta aaaatccagt gacttttggc
     4681 ccttattaca tgagtaatat cgttgcagga gaatcagtag aatacttgcc aaacgagcac
     4741 tactggaatg gtacgcctaa attgaaaaag atcacgatga aatctgtacc gatggcaagt
     4801 gcaactgaag cattgaactc taaattatat gatatgattt tccaaatgcc aacagatact
     4861 tacgatacct ataaagaaat tgctggctat acaaacttag gccgtgaaca aacttcttat
     4921 acttatctag gattcaagtt aggtaaatgg aatgcagata caaagagtgt ggaatacaat
     4981 ccagattcaa aaatggctga taaatcatta cgtcaagcaa tgggctatgc attagataat
     5041 aatgcagtag gtgaacgttt ctatgcaggt ttgagaagca atgcgacttc attgattcct
     5101 ccagtattcc agtcatacca tgatgatgag atgaaaggat tcacacaaga tctagacaaa
     5161 gcgaaaaaac tattagatga tgctggctac aaagatgtgg atgacgatgg tttaagagaa
     5221 gacaaagatg gaaataaact aacaatcaac tttgcttcga tgtccggtgg ggaaacagcg
     5281 caaccattgg cagattacta tatccaacaa tggaaagaaa ttggtttgga tgtccaatac
     5341 acaacaggtc gtctgatcga attcaattct ttctatgata aattagaaaa cgatgatcct
     5401 gaaattgatg tttaccaagc tgcttggaat acaggaactg atccaaatcc aagtggacta
     5461 tggggaccaa atgctgcgtt caactacact cgttatgaat cagaagaaaa tacaaaattg
     5521 atcaacgata tcagttcaag caaagcattt gatgaagagt accaaaaaga agcatatcaa
     5581 aaatggcaac aatatgcatt tgaagaagca tttgcgattc caacgttgtt ccgtaatgaa
     5641 gtattgccag tcaacgaccg tgtcaaagac tgggattggt cttatgatgt accaaaccca
     5701 tgggcaaatg tttctgttac agcagattca cgttcataat cactaaaaat gaccgaaaaa
     5761 gggcgatgtc ctttttcggt tttttgatat ttttctgttt atttctctaa tattcaggtc
     5821 aaggcgctga tttcatgact tctccaataa acggtgttca aagtccagtt tctcggtatt
     5881 aagtcaaatt tatgcaaaaa tatggaaagc cattttcaca aaattttttc ttcatactcg
     5941 aaactaaacg aatttttcac aacttctcgt caatcactag gattattaaa gctttactta
     6001 ccttttgacc gaaccatttc aaatttgaca aaaaaccagt ataataagga agtatcgaaa
     6061 aaatatccat aaaaggaatg tggaaaatgg acaaccagct gacaaaagaa ctaaaagaaa
     6121 actataacat cgtttttcac gatctaaatt tgcttgaaca agcttttacc cattcatcct
     6181 atgtgaatga gcatcgcaat ctgcaattat cggataatga gcgacttgaa tttttaggag
     6241 atgccgtttt agaactgatg gtttctcagt atttattccg tttatatcct catttgccag
     6301 aaggaaaatt gacaaaaaca cgagctgcga tcgttcgcga agacagcttg tcgaaatttg
     6361 ccaaagaatg tcactttgat caatatgttt tgttgggcaa gggagaagag aattcaggag
     6421 gacgtacgcg accagcattg ctttgcgacc tattcgaagc ttttttaggc gcgttatatc
     6481 ttgaccaggg attcgacacg acccatgcat tcatcgaaaa agtgattttt cctaaagtca
     6541 aagcaggtgc tttttcacat gagatggatc ataaaacaaa actacaagaa gtcttgcaaa
     6601 aatcaggtga tgtatcaatc gaataccgat tgatcaatga agaaggtcca gctcatgaac
     6661 gagtattttg gatcgaagtt tatgtagatg atcaattgat tggcactgga caagggaaat
     6721 cgaaaaaatt agcagaacaa gctgcggcgg aaaatgcatt ggcagcactg taaaagtaag
     6781 tgagggaaag ctttgtattt gaaacgaatt gaaatagccg gttttaaatc atttgcagac
     6841 aagacagtca ttgattttga aaatagcgtc acagcagtgg ttggaccaaa tggcagcgga
     6901 aaaagcaata ttacagaggc tatccgctgg gtattaggag aacaatctgc taaaagtctg
     6961 cgaggcggaa aaatgccgga tatcatcttt gccggttccg atacccgtaa gcagctgaat
     7021 attgcagaag tcacagtgat tttagacaat agtgatcatt atctgccatt ggagtacaat
     7081 gagatcagtg taacccgccg ttatcgacgg actggggaaa gcgagttttt catcaataaa
     7141 caaagctgca gattaaaaga tatccaagaa ttgttcttag attctggatt aggaaaagaa
     7201 tcgttctcaa tcatttctca aggaaaagta gaagcgattt tcagtagtaa accggaagac
     7261 cgtcgtggga tctttgaaga agcagctggt gtcttgaaat acaagcagcg gaaaaagaaa
     7321 gcagaacaga agctttttga aacagaagac aacctcagcc gcgtccaaga catcatccac
     7381 gaattggaag aacagttaac tcctttggcc gcccagagtg aggcagcaaa ggagttttta
     7441 cggctaaaag aaacactgac tcaaaccgat gtctctttga tggttgctga aatcaagaca
     7501 gcaaaaaaag attgggacaa caaacaagca cagcttgcga aattcaatct agaattaggc
     7561 aaactatctg agtcgatcca agaacaagaa tccattttgg cgaagcagcg taaagaaaat
     7621 gcgcaagccg atcgtctgat cgaaaaaaat cagcaagtat tattagatct ttcggaaaaa
     7681 ctgaagcaga cagaaggaca aaaagatgtt ttgcaggagc ggacaaagca tacacaaaaa
     7741 agctcgcaag aatatcaaac ttcattagca gaagcacaaa aaaaggttaa acattttgaa
     7801 aagcttcaag aaagcttgat gaaagcagca gcagaaaaag aaacagagat ccaaaaagct
     7861 gaagcaaatc tcataaaaac acagcaagaa ttagaaaaat accaaaaatc aacgaaagaa
     7921 ttattggcag aattaagaga tcaatatgtt gatttgatgc aagaacaagc agctgtagga
     7981 aacgaactga agtatctaga acgtcaatat atacaagaaa cagcaaaaag caagcagacg
     8041 ttagccaagc aatcggaagt agaagcatca gtagaccgat tgatcttaca aaaagaagaa
     8101 ttgacacaaa agcaggcgca gctaaaatca tctttaactg aaacaaaaga gaagttagaa
     8161 atgatccaac aaaacgggaa aaagttccaa gaagcattag caaaagaaca gcctaaaatg
     8221 tatcaattaa tgaatcaagt ccagcagcta cgtgctcgta agaaaagcct tcaagaaatc
     8281 caagaaaact attttggttt ttaccaaggg gttcgattgg tcttgcagca taaacagcag
     8341 ctttcgggta ttgtcggagc agtggctgaa ctgatcgatg ttcctgcaga tttcacgtta
     8401 gctatcgaaa cagctttagg tggggcagcc cagcatgtca tcgttgaaaa tgaaaaagat
     8461 gctcggcaag ccatcacgta tttgaaacag caaagaggag gaagagcaac attccttcca
     8521 ctgacgacaa tcaaaccaag acaattaccg gctcatatat tgacacaagc agcagctgta
     8581 gaagggttta tagggattgc cagtgaacaa gtcagttatc ccgatcaaat ccaaactgtg
     8641 gttcaaaacc tgctaggaac gatcctttta gccaaagact taactagtgc caatgcaatc
     8701 gcgcaaacga tccgctatca atatcgagtg gtttccttag aaggtgatgt gatgaatgcc
     8761 ggaggatcca tgacaggtgg agcgaataaa cgaggaaacc aaggcagttt gtttgtacaa
     8821 aatcaagaat tgaaacagtt gactagtgaa tttgaagaag cagataaaca actccaagca
     8881 caagaaaaaa aagttcagga gctgcaacaa gaaacagctc gtttagccga agaacaagaa
     8941 gttttgcgta cacgaggcga acaattacgt tttgaagaac aagaagcgac aaatcagcta
     9001 caaaacatca taaatgaact tgaacgtttt gaaaaagaaa agcaaatctc aacttttgag
     9061 acacgtgaac tgcagcagtt tatcgaagat tacgaaaagc aaacgaacga attgaaagac
     9121 aaacaaacag atcttgaatc gcaacgacaa aaaatcgacg aagaaatcaa atcgttaagt
     9181 caagaaagcg accagatgga agccagaaga gcacaagtac aaagtcaaaa agcacaagaa
     9241 caagcagatc ttgctgtctt gaaagagcag tttaatcatc tgcagatcca gctccgcggt
     9301 gcccgagtcc aaaaagcaga ggcaaccgaa cgtcaagaag cgatagaaaa gcagttggcg
     9361 acattgacag cagacttttc tgatcatgaa gtcacagaag aaagtttgga aaaacaaatt
     9421 aacgaattat ctgctcagcg agaaacatta aaagccgagt tggcaaaagc caaagagcag
     9481 cgagatagaa ggcaaaagga gatcgataag cttgaagcag tcttggcaga gcgtaatcgt
     9541 gagcaaaaag cacgactgtc ggaacaatcc aaattagaag tccaaaaaga ccgagctgaa
     9601 atgattttag ataatcattt gacctatctg cagtcagaat atcagctgac atttgagaaa
     9661 gcttcccaag actatcaaga aacaaccgat atcgaagaca gtagaacaaa ggtttcatcc
     9721 ttaaaagaac agattgaaaa actgggacca gtaaacttaa attcaatcga acaatacgaa
     9781 caagtcagtg aaagacatac attcttagca acacagcgag atgatctgtt agcagcgaaa
     9841 aaccaattgt ttgaaacgat ggatgagatg gatgatgaag tgcggacaag attcaaagaa
     9901 gtatttgaag ctatccgtca agaattcaaa attgtctttc caaatatgtt tggcggcggt
     9961 aaagctgaat tagtactgac ggaaccaact gatttgctga aaacaggtat tgagattgaa
    10021 gtacaacctc ctggcaaaaa actgcaaagt ctaagtttgc tttctggagg agaacgagca
    10081 cttacagcca tcgcgttgct cttttccatt atccgtgtct gtcctgttcc gttttgtatc
    10141 ttagatgagg tagaagcagc gttagatgaa gccaacgtga aacgttttgg tcgttacttg
    10201 agcgatttcc aagatgacac acaatttatc gtagtcacac accgcaaagg aacgatggtc
    10261 gcagcagatg tactgtacgg agtgacgatg caggaatccg gtgtatcaaa aatcgtttct
    10321 gtacggatgg aagatatcaa tgaagaggcg aaaatcaacg aaacgaaagt gtaggaacga
    10381 aaatgatcaa attagtagca atcgatttgg acggtacatt attaaatgaa caaaagctga
    10441 tttcagatga gaataaacaa gcattagcac aagcaaaaca gcaaggggtc aagatcgttc
    10501 tatgtacagg acgcccctta gctgcgatgg cgcattatct gcaagaatta ggactggtag
    10561 atgaaggtga ctacagcatc actttcaacg gcggtttggt acaaaaaaat gatactggtg
    10621 aaatcatgga aaagaaagtg atggaagtat cagatatcca acgcttatac aaactggctc
    10681 agcaattgga tctgccacta gatgttttgt ctgacaatgt ggttttacag ctgcctagtg
    10741 ccccaaggaa gcaatccttg tacaatgtgt tgaataagct gctgcaattc caaccagcgg
    10801 atataagcga tattacagat gaatttgtgc ttaataaggc agtaatcgct tatgaccaag
    10861 aagaattaga tcctaaaatc aaagaaatac cagcagaatt ccatgaaatg tatgagatca
    10921 tcaaaacaag aagtatgctt ttggaattta tgccaaaagg tgttacaaaa gcttatggta
    10981 tttctttatt ggcaaaagat cttggtttga aacaagaaga aatcatggcg atcggtgatg
    11041 aggaaaatga tttaccgatg atccaatatg ctggactagg cgtagcgatg gagaatgctg
    11101 tgcctttcgt caaagaagca gcagatttcg tgacagccag caatgaagaa aatggcgtag
    11161 ccaaagtgat ccaaaaattt gtattagagt agggggagtc aaatgggatt ttttgataaa
    11221 atcaagcgtg cttttactgg cgaagagaaa acagaagaag ttgaagtaca ggagaagtac
    11281 gacaagggtt tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga gttgtttgcc
    11341 aattttcgaa cagtcgatga agactttttt gaagaattag aagaaacact tatcggtgca
    11401 gatgtaggtt ttgaaacagc gatacgaatt accgaagcat tacgtcaaga agtgaaactc
    11461 agaaatgcga aaaaatcggc agaagtccaa aatgcgatca tcgagaaact tgtggatctc
    11521 tatgaagcgg aaggaatcaa tgaagtaaat gaactaaata tccaagaaga cggactaaca
    11581 gttattttgt ttgtcggtgt taatggtgta gggaaaacaa caagtattgg taaacttgcc
    11641 catcagttca aaaacgaagg taaaaaagta ttgatggcag cagcagatac cttccgcgct
    11701 ggagcaatcg accagttagt tgtctggggc gaacgcgctg gtgttgaagt tgttcgtggc
    11761 aatgcaggag gcgatccagc agctgtcgta ttcgatgctt tggaaagagc aaagacagag
    11821 caggcagatg ttttactagt tgatactgca ggccgactgc aaaataaagt gaacttgatg
    11881 aacgaattag aaaaaataaa acgagtaatc cagcgggaat taccagaagc accccaagaa
    11941 acacttcttg tattagatgc aaccactggt caaaacgcga tggttcaagc aaaacaattc
    12001 aaagaaacaa cagatgtgac cggtcttgtt ttgacaaaat tagatggtac agcaaagggc
    12061 ggtatcgtac ttgctatccg taatgaattg catttaccag tgaaattggt cggtcttggt
    12121 gaaggaatcg atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg attgttcaaa
    12181 ggattactaa aagaagaata acccatgtct aaaaagcagg agctgtgaca aaagttgcag
    12241 ctccttgttt gaatgattgt ttttaacata gctgtgtttt tgatgccttc atttttatac
    12301 ttatttgtaa gcggttctca aacaatttcg ttctttttct tagacttttt gattataatt
    12361 agattcatat gaattggagg tcacttaatg atgaatttta agcaattatt attgcatgtc
    12421 aatgcgcgcc cttttatcga ccaagctcga tttggtatag aacgggaagg acagcgggtt
    12481 gatcttgcag gaaatctagc aaaaaccgat catccagcaa tctttggcga tcgatcctat
    12541 cacccctata tccaaacaga ttttagcgaa acacaaacag agatgatcac ccctgttacc
    12601 gattctattc ccgaattatt tcagtatctc gctgctgttt atgatgtgac tgctcgttct
    12661 ataccgaaag aagagatgat ctggccatta agtatgccac ctgccttacc ggaaaaagac
    12721 gaagagatca ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg acgttattta
    12781 gcaaaagaat acgggaagcg aaaacagatg gtaagcggta ttcatttcaa ttttgaattt
    12841 ggcgatgagt tgctaagaac attgttcagc catcaggaag aatttcaaga tttttccgaa
    12901 ttcaaaacag aactttattt gaaaacagcg agaaacttta tgcgctatcg ctggatgatc
    12961 acttatttat tcggtgcttc accaatgagt gagaagaact actttttaga tgaatcacat
    13021 ccgcaagaac ctgttcgcag tattcgcaac agtgcgctgg gctatacgaa tcatccaaat
    13081 gtgaaagttt cctatgcttc tatgaaacag tatttagcag atatcgagcg aatgatcgaa
    13141 gaagggaaac tttcggagga gaaagaattt tatacaccgc ttcgtttccg aggcggaaag
    13201 aaagtcgcag atttagcaac aacaggtgtt cgttatattg aattgcggaa tatcgatttg
    13261 aatccttatg caagattggg tatcaatcca gagcaagttc ggttcttaca attattcctg
    13321 atgtacatgt tatggacaga agaaaaagaa gactgtgacc aatgggtagc agaaggtacg
    13381 actcgaaata acaaagtagc actagaacaa ccatcagatc aaacagaatt tcatcaagaa
    13441 ggtagagaaa ttcttgaagg aatgaagcaa atgctggttg aattggattg gttggattct
    13501 ctttatttag tagaggaagc attgactcaa atggatcatc ctgaacaaac gttagcagcg
    13561 aaactctatc aagaagcaca gctatcgagt cagcaagaag ttgctgtggc attgggacat
    13621 caatattata aagaaagcca tgaacgtccc tatcagttgg ccggttttcg cgaaatggaa
    13681 ctttctactc agatatttat gttcgatgcc atccaaaaag gtgtccaagt caaagtatta
    13741 gatgagtcgg atcaattttt gcgtctgcaa ttccaagacc atgtagaata tgtgaaaaat
    13801 gcaaacatga caagtaagga cagctatatc gtcccactta tcatggaaaa caaaacagta
    13861 acgaaaaaag tcttaaaaga agctgggttc cgggtaccag gaggcgcaga attttcatcc
    13921 atggaagaag cggtaaaagc ttatccgaga tttgccgagc aggcatttgt gatcaaacct
    13981 aaatcaacca attacgggtt aggtatcacg attttcaagg aaggagctag tctggaagat
    14041 tatcaagcag gtttagccat tgcttttcgt gaagacagtt cggttttagt agaagaattt
    14101 atgccgggaa cggaatatcg tttctttgtg atcgacggag aagtccaagc catcatgttg
    14161 cgagtccctg ctaatgttat tggtgacagt atccgaacag tgaaagaact tgttgaagaa
    14221 aaaaatagtg accctttgcg tggaacgaat catcgtgcac ctttagaatt gatccaatta
    14281 ggtgaattag aacaattgat gttgaaagaa caagggttga cgatcgaatc agttccccaa
    14341 gccaatcaaa ttgtttatct aagggagaac tcaaatatca gtacaggtgg tgattcgatc
    14401 gatatgactg atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc ggtagaagca
    14461 ctaggagcta agattagtgg tatcgatttg attataccag ataaagaaat cgatcctaca
    14521 actgataaaa aagcatatgg gatcatcgaa gcaaatttca atccagccat gcatatgcat
    14581 gtttatcctt ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa attgttatat
    14641 cctgaagtat tttaaaagaa aaagcttttg acagattgtc ctatttgatg tcaatctgat
    14701 caaaagcttt ttttgcatgt aaaaatttat gtgttttttc gttttaaaat aaacgatccc
    14761 agaagctttt tttctttttt ggtttttctt tttcagctgg cgaagagttt tcttctgggt
    14821 attttttttc gatatcaatc acaggttcat ttactgcttc ttttgctgtt aagagcaatc
    14881 cgaattcctc aggatttgca gtgacgtaat cgttgactac agtgaaacgg ctattgtttt
    14941 tcgttgctag ctggatatat tggttttgga cagaaagggg tagagcccca ttcaagagta
    15001 tggaagcatc tggatgtttt ttgagttctt ttaaaaagtt ttctttgttt tttggttgga
    15061 tcatttcagc aacagtcata ctaagatagc agcgttccct gaatgtccct aaatatttat
    15121 gctgttcttc agggttcact aaaggtgtac cataaagtcc cttatccaaa tggcttttta
    15181 catcatcttg tgccataaaa aataccctcc ttcttctctt tgtctttctt acgattatag
    15241 catattccca ataataccta aaacatatga aaaagcggtt gcttttgttc aacgattcac
    15301 ttaaaataag aatgaaaatc attctcaatt agaaaggtgg attggatgcg ctacggacga
    15361 ctagtagcat tattagttat tttaagtagt atttcattgt tcatcggagt atcgaatgta
    15421 caattgtcag aactgctcca gttagatggg caaagcttac aaatcttgct agtgactcgt
    15481 ttgccacgaa cgatttgttt gatccttgtg ggagcgacca gtagtatttg tgggttgatc
    15541 atgcaacatt tgacccaaaa taaatttgtt tcaccaacga ctgcgggcac gatggatagt
    15601 gcgcgtttag gtattttagt ggctatgatt ttcctacctg gtgcatcctt gattgtccga
    15661 tccttgacag cattttgctt tgcttttgca ggaacattgc tttttttatc ccttacgcga
    15721 ttgttcctcc agaaaaatca attgatgctt ccgttgattg gagtcatgtt agggaatatc
    15781 attggttcag ttgctacttt ttttgcttat caattccagt tggtacaaaa tatgacttca
    15841 tggcttcaag ggaattttgc gactgtgata cgaggaaatt atgagttatt gtatgctgtt
    15901 gttccattgt tgctcttggt ttggctattt gcttaccgat ttacgatcat tggtctgggc
    15961 gaatcgtttg cagtaaatct gggaatagat taccagaaga tgcagttttt aggcattggc
    16021 ttagtatcat tggctagtgc agtcatgttg atcatggtag gaagcattcc ttttatcgga
    16081 gtgatcattc cgaatcttgt ctctagaata tatggagatc aagtgcacaa aacaattggc
    16141 gtaactgctt tagcaggtag tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg
    16201 atttttcctt atgaaatacc agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc
    16261 ctttatcttc tgataagggg acgacgatat gcttaaaaaa aagccatcta tttttattgg
    16321 tatgcttgtt cttgttttat ttgttacagg aatgtacttg acgatccata catatggaaa
    16381 ctggcatttt gcatgtgcat tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct
    16441 agctgtcagc agtgccacaa tcagctttca gacaattacg cataaccagt ttttgactcc
    16501 ttcagtattg ggacttgagt cgttatatgt ccttgtccaa acggtccttt tcttctttat
    16561 cggaggagtg acgatgcttc agcagacagg tacagttttt ttcttattga acgttgggct
    16621 gatggtcggg ctgagtctat tgttattttt ctttcttttg aaaaaaatga gagcgaatct
    16681 ttttttatta ctgatgtcag ggatgatttt aggtacgctt tttggtagtg tcagtacctt
    16741 tttacaagtc atcatggatc cgaatgaata tgatttgctt caaggcagat tatttgcaag
    16801 ctttggcaat atccagagtc agtatctttt gcctgcttct ttgatcatta tcggctgctg
    16861 gtacctgtta tttcgatatc gacatgtgct agacgttttc catctcggaa atgaacaagc
    16921 agtaaacctc gggatagaca cagatcgttt tgaatttttg attttatgtt gtgtcagtct
    16981 tttgacaggg acatcaacgg ctcttgtggg tccagttact tttttagggt ttattgtcgc
    17041 aaatatcagt tatcgtgtct tttctactta tcgtcatggt cagctattta tcggaagctt
    17101 tctggtagct atgctgtttc tgacaggagg acagctcgca gtagagcaga ttttcggctg
    17161 gaatacaaca gtgagtgtcg tcgttgaatt tgtcggagga atttattttg tcacaaaatt
    17221 gattcgcgaa aggaagagta tcctatgatt gatttacaaa aggtcaataa actgtaccat
    17281 ggaaaatcag tggttaaaga tgtacagtta acgattcctg aaaagaaatt gactgctttt
    17341 atcggtccaa atggtgctgg aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca
    17401 aaagatacag gtgaaatcta tttagaccat aacgaagtaa aggcttggaa atccaatgaa
    17461 ttagccaaaa aattatcgat cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg
    17521 aaagaattag tagaatttgg acgatttcct tatacgaaag gaaaattgac gacaaaagac
    17581 gaggaaatcg ttcaggaatc actggaacac cttgggttag aagaaatagc ggatcaatcg
    17641 attgacactc tttcaggcgg tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa
    17701 gacacggatt atattttact ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt
    17761 caaatcatgc agattttaaa aaatttagta caagaaatcg ggaaaacaat tgttattgtt
    17821 cttcatgata tcaactttgc tgcaagttat gcagacgaaa ttgttgcaat gaaagatgga
    17881 cagttattta cgcaaggaac gacccaagaa atgataaaaa aagatgtatt ggatgaatta
    17941 tacgaaatgg acatacggat ttgtgagatt gaaggccgca gattctgtct ttatttctaa
    18001 aactaacatt aggagagaag agatgaaaaa attagtagta ggtatcttga ttctagcatc
    18061 atttggttta gcagcatgtg gaaatagcaa caacaccagt caggcagaca caaaaaaaag
    18121 cagcacacaa actgaaacta cattgacgat aacagatagt aatggagacc aaatcgaggt
    18181 gccaaataat ccagaaaaag tcgtcgtatt cgacaatggt tctttagata cgatggatgc
    18241 tcttggtgtt ggagataagg tagttggagc agctacaagc agccttcctg agtatctttc
    18301 atcctataaa aaagtagaat cagcaggtgg aataaaagaa ccagacttag aaaaaattaa
    18361 tcagcttcag ccagatctaa tcatcatttc tggccgacaa agagattttc aaaaagatct
    18421 aagtcaaatt gctccgacca ttttcctttc tttagatgcg aaaaatccat gggaatcttt
    18481 ccagcaaaat gtcactgctt taggggagat ctttggtaag caagaagaag ctaaaactca
    18541 attagaggaa ttatctagtg caatcgatca gacgaaaaag aaagcagaag ctactgacaa
    18601 aaaagcacta gtcacacttg tgaatgaagg gcaattatca gcctacggat ctggttctag
    18661 attcggtttc atccatgatt tatttggctt tgaacaagca gatgatcaaa tcgaagcatc
    18721 cactcacggt caaagtgttt cttatgaata tgtgctggaa aaaaatccgg atatcttatt
    18781 tgtagtagac ataacaaaag ctattggtgg agacgacagc aaagatgata tttctgccaa
    18841 tgaactggta gcacaaacga atgctggtaa aaatcagcag atcatctccc ttgaaccaga
    18901 tgtctggtat ttaagcgggg gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa
    18961 tcaagcattt aaatagcata aataacagag gattaaactc tcatactatg taacaataat
    19021 gtttcttaca ttcttaaata gcaagagcct atcctcaaag aagttatcaa aaaccatccg
    19081 taaaatcgta ttgatgattt tacggatggt ttttatttat ttgagaaatc ttttttcaac
    19141 gtgaattcat tatctacatc atacgggttg attctagaat ggggagtggc aaaaaatagt
    19201 gtgatccatt ttttcagcat caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg
    19261 aaacttagat aaaacttaaa tttagatatt tttattttgt accgatctct tctttaaacg
    19321 atgttccatc tgctgcttct cggcaataag tccactagac tagaaaatat gagaagctat
    19381 tttctgtcca gcgttcttat tactcaaagc aaatcgcagt tgtcacaatc tcttctaaac
    19441 gatgttccat ctgctgcttc tcggcaataa gcccactaga ctagaaaata tgagaagcta
    19501 ttttctgtcc agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa
    19561 caatgttcaa tcagctgacc ttcggtatta gctcaaagca ctgatttcac aacctctatt
    19621 taggcatgtg tctcagcaag cggttgatag ttgatgatct gcatttttcg ataaaaatcg
    19681 gcgggaagct tactccaccg ttttaataat tgatagaaat aactctcttc tgctttgatt
    19741 ttatactcgc ttaatatcaa tccaaaacta ttcgtttttt ttgaagctaa ttttttataa
    19801 atatccaatg ctttggtttt atcttcattg acaaagagtg tttttacttc aatataagaa
    19861 ccctcgatat tttgtgtttc ttcgataaaa tagatgtttt ttatatattc cttcatagtg
    19921 attcctccaa agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc
    19981 cgtgaacaaa aagtaattct ttttccaata gttgttaaag tttttttatt ttttatagta
    20041 ttttttgtta tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt
    20101 tttccgttca agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa
    20161 tcataataaa cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt
    20221 agttgcatct gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag
    20281 aagtgacaaa agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt
    20341 tattagatat cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg
    20401 atacggcatt tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt
    20461 ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca
    20521 tggatgatcc atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac
    20581 caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg
    20641 gaaaattggc gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc
    20701 tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag
    20761 atacggctaa taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat
    20821 tatctacgac atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga
    20881 tggaccattt tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga
    20941 ccgttctttt agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg
    21001 aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa
    21061 cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg
    21121 aagcaaaaaa acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca
    21181 tgtatcaatc tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat
    21241 cattcggaga attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc
    21301 atcgtcgctt tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt
    21361 attattatga atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct
    21421 atgatcctat gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc
    21481 aaacacaaca tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca
    21541 gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag
    21601 atccatttat cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg
    21661 atcggatgat ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga
    21721 taatcacaaa aaaaaatata atcgtaaatt tcaagtttaa gttagccacc catggtgacc
    21781 cggttgctcg ttaccaccaa ccgtctaatt tttctaatcg agtgggccct tgacaatgag
    21841 ctgaggcatg gtagtctccc gactggctgg ccccggccag ctttccagac acaagccatg
    21901 ccgagggagg ctcgttgtca agggcccacg tgtcaagccg tcaaccgttc ctgcctaaat
    21961 gcctggacga acgtgtcatg cggcgttgca tagcccaaaa tctttcgcgg gtaatcgttc
    22021 atccaacgct gaatccgcaa gatttggttt tcgctcacgt tgccgattgg gctgcccttg
    22081 gggatgaaac ggcgaatcag cttgtgttgg ttttcgctcg tcccgcgctc ccaggaagca
    22141 taggggtggc tgaagtagat gtttgccaca tcctgcaagg catcgtgcag tcccgcgaac
    22201 tccgagccgt tgtccgaggt aaccgtcttg aacagctggc cgaagtacgc accagcccgt
    22261 tctctcagct gtcggaccgc ttggttgacc gattcggcat ccttcccggc cagcttcaga
    22321 atgacctcga agcgtgtctg ccgttccacc aacgtcagca gcacggcatc cgacttggtc
    22381 ttgtttccta cgaccgtgtc gacttcccaa tggccgaagg ttttccgggt accaaccgcc
    22441 tccgggcgct cttcaatgga tttccctagc acccgtttat tcgggcggtt gccatgacgg
    22501 gacgatttcg gtttgcggga cagtttttct agcaggtcca tattcttcgt tttcatgatg
    22561 ccacggtcga tccactgata gagggtggtg gtcccaggga taatggccgg gtcgaacaat
    22621 tcgtgttttc tggcaaagcc caccaccacg tccggggacc atttttccaa cagcatcatg
    22681 tcgtccgccc attcaatgaa ggcgtccgtg tcggcccata agggccgtct gccgcagttc
    22741 aagcggttct tctcgtaggc cgcctgcccg gcatcggcgt cgtagacgtc atgataatag
    22801 tcgtagacct tcccgttctg attctggcgg ttaagctgag taacggtccc gcgtttgatt
    22861 tcgttgttta tggtctgcgg cacgcagccc aggacatccg caacctgacg gttggaatag
    22921 ttttcctttt tcaggatggc gatttggcaa cgttctgagt aggttaggtg ttttcccttg
    22981 cgtgaaactg tgttatcatg gttttgcgtc atgtgaattc atccttgtct attgagagtt
    23041 gtggtaactt caatataaca tggaattcac gtggcgtttt tttatttcca aatggctaag
    23101 ttaattataa aatccgcctt tttttaaaag taacaaaatt tcgaaaaata tgcaacaatt
    23161 ttcagaaaat tccctatata aaacgatggt tatttaataa cgtaagtgat aaaataaaag
    23221 cgctgaatca tctgaggagg aatcatcgtg acaaagtcga gattagaagc atttactgat
    23281 ggagtggtag ccattgtttt gagagttatt gtattagaga tcaagatccc taatacatct
    23341 agctttcatt ctttatgggt catccgaaat acattattag cttataccat cagctttata
    23401 tttgttgcgg tgatctgggt gaaccatcat cgtatatttc agatggcaga acgaattaat
    23461 taccgagtgg tctggtcgaa tatctttttg ctcttttggc tgactttatg tccttctgtt
    23521 actagttggg tcgggcgtaa ccctgaagct ttttggcctg agatggctta tgtcttagta
    23581 tacacgatgt ggagtttttc ttttggtatc ttgatgaggc aaatcatgaa agcaaataaa
    23641 ccagattccc atgtcgtgag agttttgtca aaggaccgtc gaagtcttat ctccatgatg
    23701 attaacttat gcttgattgc cggtgtcttt tttgtaccgt tgatcgggtt attcggacgt
    23761 ttttttgtat ctggtatttg gattttttcc tataaaaaag cagatgccta ttatcagcgg
    23821 ttgtttccgg ggaaatagaa aagaggctgg gacagaagct tttatctcca agaaataagc
    23881 cgaaatttca caaaaattta agaagcaatt ttcgtaaatt ccttcttatt tctcgaagct
    23941 gaccacttct gtcacaacta ctttcttact tcttacgttc atatttcata tgatagatta
    24001 gtaatagaag caagaaccac acaggtgtta gtaataaagc gattctcgta tcttttccta
    24061 ggaacaagac gacacacaca aaggccatgt aaagaaatac cagataatta ctaaatgggt
    24121 acaacggcat tttaaatgaa tgagcttttt tgccgaggat acttttacga tatttcatgt
    24181 gcgtgtagac aagcatactc cagataaaca agaaacaagt agtggcaata ctgcttacta
    24241 gtgtgaatac ttcactaggc atcacgtaat tcaagataac agaaatgaaa atgacgatag
    24301 tagaaaatgt caaagcattc cctggcacat gatgtgttgt taactttttg aattttttag
    24361 gggcactgcc ttcttgcgct aaagaacgaa gcatacgtcc tgtactatat attgcactgt
    24421 tgcaggcaga agctgctgca cttaatacga ctaaattgat caaggaagca gcaatcgtga
    24481 tcccaatttc cgaaaatact tctacaaatg gactgttatc tggattcaag ctgttccatg
    24541 gatagattgc cataatgaca aaaagagatc ctaagtaaaa taagatgatc ctgataggaa
    24601 tattattgat agcttccggc aacacttttt caggattttc tgtttctcca gcgactaatc
    24661 caactaattc tatcccagca aaagcaaagg ttaccatttg gaaagctaag ataaatcctt
    24721 ttgcaccagt tggaaagaat ccgccatgag accatagatt cgtgatactg gctgtgccaa
    24781 tatctgtcgt atagtgggtg aggatcatat aagctcctac gaggataaag gcaatgatcg
    24841 ctacaacttt gatcaatgca aaccaaaact ctaactcacc aaacaatgag acagccacca
    24901 tattcaagca aagaagaaga cctaatgcaa tcagttcagg aagccactga ggaacaccag
    24961 gcgcccagta gcgtacatac attccgatgg ctgttaaatc ggccattgca atcgataccc
    25021 agcagaacca atacgtccag ccagtgatga aagccgcttt tttgccaaga tattctgcaa
    25081 caaaatccag aaaagaatgg ttattggtgt ttgataacag aagttcgcct aacgcacgca
    25141 taatgaaaaa acagatgcat ccagtaataa gataggcgag caatatcgaa gggccagcta
    25201 gctggataga ctttccggaa cctaaaaaca accccgttcc aatcgctccg ccaatagaaa
    25261 tcagttggac atgtctattt ttcagtccac gctgcagttc ttgctcttca ttcattttta
    25321 aatcatccta actaaaaaag tttaattact tccatattaa gagagttttc tgaaaaacac
    25381 aactaagatt ccaaaatttt cagaaattta ccttttatga gtaattcata aaaaagaact
    25441 gcggtaaaca ccgcagctct ttgtcccaat cagctattga tcctaagcat ttaaaatacg
    25501 tgaaaggaat tcttttgtac gttcttcttt cggatggaca aagatatctt ctggtgtacc
    25561 ttcttctgca atcacacctt tatccataaa aatgacacgg tcagatacat cttttgcaaa
    25621 ttccatctca tgcgtcacga taagcattgt caagccagta tgtgctaagt ctttgatcgt
    25681 attaagcact tcgccaacca tttcagggtc taacgccgaa gtaggttcgt cgaatagcaa
    25741 tacatctgga ttcattgata atgcccgagc aattgcgaca cgctgtttct gtccaccaga
    25801 taattgtgct ggtttggctt cgatataacg atccatacca actttttcca gattttcgat
    25861 tgctactttt ttggcagttt ctttatctcg tttcaggaca gttgtttgac cagacataca
    25921 gttttccaag acattcatat tgttgaacag gttgaaggat tggaaaacca tacctagatt
    25981 cgttcgatat ttaggtaaat tatatttagg agctaataca ttttctccat tataaatgat
    26041 ctcgccgcct gttggttttt ccagtaaatt gatacagcgc aagaaagtag atttaccaga
    26101 accggaagaa ccgatgatcg tcacaacttc acctttattg actgttacgc tgatatcttt
    26161 caatacttca ttttcaccga agctttttct tagatgtttg atttcaataa ttttttcatt
    26221 catgattctt cctccttatt cctgcaaatt tgaattgtcg atttcttcaa gcttggtata
    26281 agctgaagga ccatcgattt tcttttctac cgcccgcagg attctggtga tcgtaaaggt
    26341 catgactaag tagatgatcc cgacgatcgt aaatgtttgg aagaattgga aattcgcacc
    26401 ggaagcagag tttccttgga agaacaaatc agctacgccg attacactta aaactgctgt
    26461 atctttgata ttgataacaa actcatttcc tgttgctggc aagatattcc gtaaaacttg
    26521 tggaataaca acttttctca tcgtttggcc atgggtcata ccaatggctt gcgcagcttc
    26581 gaactgccct tcatctactg caaaaatccc cccacgaacg atctctgtca tgtaagctcc
    26641 agtattgaga gagacaataa acaaagcggc aatcgttcgg ttcaatgaaa tgccaaaagc
    26701 gagtgccaga ccgtagaaga tgaccattgc ttgtaccatc atcggtgttc cgcggaatac
    26761 ttcgacatag atagaaagaa gtgcatttcc gattttttgg aagaagcgag tcagtgcatt
    26821 tcctgattca ggtaccgaac ggaagacacc aatcagtaat ccaagtgtag taccgacaac
    26881 tgtcccgatc aatgcgatga acaaggtaag tccagcacct cgtaagaaca tattgccgta
    26941 ttgtttccaa atcgtagtga aatcagaaat gatgccaggt ttttcttcac tatcagttgc
    27001 agcaggctga tctttgactg cttggtccat aattttggtt cgttcatcgt tagagatgcc
    27061 agaaagaatc tggtttactt tttggatatc cgggtcgcct ttacgcatac caacagaaat
    27121 ctgaacatct tccgggtttg tctggaagcc atctgcaccg ctgaattcaa gcatttccaa
    27181 gtctttgttc acactagttg ccgttacacc ttccggacgt tcgctgacat aaccatcgat
    27241 cattccagaa gctaaagctg ttctcattgc tgagaaatta tccattgctt gttgtttctg
    27301 cacatctggt atctgatcga tcacagtata gtggaatgta tttaactgag cagtgatctt
    27361 tgctccagaa agatctgcta aactttttgc ttttgcatat ttgctatctt tttgaactac
    27421 tactactaat tgtgattcgt aataaggatc ggtaaaatca atttcttttc gtcgttcagc
    27481 agtagggctc attccggcga tgatcgcgtc gatcttgcct gattgaaggg caggagccag
    27541 accatcccat ttcgtttgga cgatgactaa ttttttccct aaaccatctg ctactttttt
    27601 agcgatttga acatcataac cgccggcgta actattttgg ccttgaattg gcaaggcatt
    27661 attctcatct gtttgttgag accagttgaa gggtgcatac ccagcctcca tacctacgcg
    27721 aaactcatcg ctcggtgttt tttcatctgc atttgccaga gatcccacac tgaatagtga
    27781 gaagacaaaa gcaaatagta cggcaactga aaccatattt tttttcattt ttttctccct
    27841 ttctgattat atgatacacg agcggtctag atgaaatgcg gtaaagcttt atctgaaaaa
    27901 ataaaaagcg accgatttat agatataaaa aggccgctaa aattccataa ggtgataaac
    27961 ctctcacaaa acatagcgca acttagcatc actgctaaga cagtccgcaa gctcttaact
    28021 tgcgtcccaa catacactcc aagcaaaaaa gtgcacattt cggcgatcct ccctattcct
    28081 gcttctccat gtttgcttca ctccacaggg ttaatgattc tcgcgacctc tacctcattg
    28141 attgaggcat ctcgtattca gttatttata caaaaaaagt ttacgatact cgagaggctt
    28201 tgtcaatcta taactgctgg tttttgaaaa aatactgata aatatagaaa tctttacttt
    28261 taccgaaaaa acaatcaaaa tgtgaatcat ctatccattg caaacgatag tataattata
    28321 cacaaaaatg tatgtaatac aataaaaaaa gaactagaac agaaaatttc ctgtgtccag
    28381 ttccatctcg atcatttaag ctgccgcttt ctttggctca cgtcctagga tcgcttgcaa
    28441 aagaacaacg acgccataaa tggctaatat tagataaatc aaacttgagt attgggtgat
    28501 tccccagccc catgttcgat atacggctaa tgtaatggct aaaacaattg cggcaatcac
    28561 gttgaataaa gcaaaagccc aaagatttcc attttctttg cgtacaagat aaaaaacaga
    28621 gaaataaacg cttaacgcta accaggcgat acaagccaga ataacgcccc aataaataaa
    28681 gaatggtaac atatcgattc acctcacttt tatacagcag tattgtaaca cgttttcatt
    28741 cttttgtcag tgaacattgt gataaattgc tcaaatgccc ataacacttt tttattccca
    28801 tttttctgac ttttcaacaa taaatttgtt cgagtatggg ctataatgga aagtgaggtg
    28861 acaaaacatg aaacaattaa cagtagacga agcagcaaaa gagatattag cttatcagca
    28921 aattacattt ttgaccggtg caggggtatc cacaccttct ggagtgccag actatcgatc
    28981 tttgtcagga atctaccacg ggatggagca gccggaatac ttgctgagtc atcaagcaat
    29041 ggtgaatgaa ccggataaat tctatcaatt tgtcaaaaag atttatcatc ctgaagcaaa
    29101 acccaatatc attcatttgg aaatagcaca tctagcgaaa gaaaaaaatg tttgggttgt
    29161 ttcccaaaac atcgacggtt tgcacgcaaa ggctaaaagc ccgcaactcg tcaattttca
    29221 tgggagtctg tatcaatgtc attgtcgaaa atgtcaacaa acggtagact ggaaagaata
    29281 tcttcacagc gacaaacatc aagcatgcgg aggacaaata cgcccagaca tcgtgttata
    29341 tggtgaaggg tttcaagatg gagtcatgga gcaggcggct tatgccgtca gccaagcaga
    29401 gctgatcgtc attgttggaa cgagttttca agttcatccg ttttgtgatc tggtacagtt
    29461 tcgccagccg tcagccaaaa tcttagtaat caatcaaacg cctgtttatt tagcggagcc
    29521 ttatacgttt attcaagaag acggagcggt tgttttcaaa aaaatacaag aattaggagc
    29581 agattatgac tgaattcaaa accgaaaaag aaaaaatgat tgcaggcgag ctttattttg
    29641 ccaatgatcc tgagctggtt gccgaccgta tgtttgcaag aagtcaaagc caaatcatca
    29701 accaagcaga atcagcggaa ctgcgatccc aattgttgaa agagactttt ggccgtacag
    29761 ggaaaaaaat ctatatggag ccagttatca attttgatta cggatataat atatttgttg
    29821 gggagaattt ttatgcaaat ttcaattgta catttttaga tgtcagcacg attgaaatcg
    29881 gtgataactg catgttcgct ccgaatgtcc agctttacac ggcgacgcat ccactacatc
    29941 cagtgaaacg taacagcggt ctggagtacg caaaacccat taaaatcggt aataatgttt
    30001 ggttaggcgg aggagttatc gtcacgccgg gtgtcactct aggggacaac gtagtagtcg
    30061 gcgcaggttc ggttgtgacc aaatcttttc ccgacaatgt agtgattgca ggaaatcctg
    30121 ctcgtatcat caagacagta gaggaagaac taacagaaga gtctcttgaa acattacgtc
    30181 ataagatcga tatgatcgat cgagagttgg ttgctttatt agaaaaaaga atggatacgg
    30241 taactaaaat aggacaagtg aaaaaagaag aaaaccaagc agtttatgat gcaaaaagag
    30301 agcagcaagt attggataaa gttgttagtc tgctgaaaaa taagggatat aaggaaacaa
    30361 taacagacac gtatgttgat ttgatgaaac actcgcgcga ataccaaaac aaaatgaagg
    30421 aggaataagt gtgatcgaag gagagacacg gcgaaaacaa atcatgcaaa ccttggcaaa
    30481 tacagaaaaa ccagtgagtg ctagcaagtt tgcgaaaact tttggagtca gccgtcagat
    30541 cattgtaggc gatattgctt tactacgagc tgcaggtaaa agtatcgtgg caacagcaag
    30601 aggttatatc ctcgaaacag aagaaaccaa tactgggcac atcagtaaaa tcgctgtcca
    30661 gcatggaaaa gatcaaacag aagaagaact gagactaatc gttgaaaatg gtggtgaaat
    30721 cattgatgta attgtggaac atccccttta cggtgagtta gcaggaactt tgcacatcaa
    30781 aacgccacaa gatatcgatt cctttatgag acgttatcaa aaaagtaatg ctactttgtt
    30841 atccgaatta acaggcggca tccatctgca tacgatccgc tacccagaaa agcatatgct
    30901 gaaaaacatc aaacaaaaac tagctgaagc aggaatcctc tacgaaggat aacatcgtag
    30961 ctgctacttt ttatcctaga aactattcta tcatctctga gataaacacc catatggaag
    31021 aaataagaaa aaataagaaa agatgcgtat ccaatacaca gaattttttg gaattgcgat
    31081 ttctagatgt ctatctaaat ttcgattagt ggagaaacat aaaaaaggga agtgcagctt
    31141 ttgagcactc ccctttttta taaagctttc ttgtagcata gtatcgaagg aacctgccaa
    31201 ttctctactc gatcaccaat acgccttctt ttagtgcaaa tggattttga tcgttgatgt
    31261 ggtcatagaa catcacaccg ttgatatggt cgatttcatg ttgaacgacg atcgattcat
    31321 aattcttcaa acgaatcttg tgtttttcac cattcatatc ataataagac acagtgattt
    31381 tggcatggcg aacaacatat cctggtactt ctcgatcaac agataaacag ccttcacctt
    31441 cccctaaaca agcatcttga acagaatggc tcaaaatttt aggattatac ataacagtac
    31501 ttagagaagg tccatctgcc tctgggtcag gacttggtac gtgaacagca atgattcttt
    31561 tagaaatatc taattgagga gcagctaagc caacaccgcc tcgtaagtgt agttcttcag
    31621 ctttgattgg atcttggctg ttttttaaga actcaagcat ctcttttcct aaagagatat
    31681 cttcttcgct taatggaaga gaaacttctt ttgcaacttc gcgtaaggtc ggatttcctt
    31741 cacgaatgat atcatccatt gtaatcatgg aatatgtacc cctttcttaa aagactgtgt
    31801 ttcaagtcta ttttcgcatt atagtttacc acaaaaatgg aaaagtagta gtaagattgt
    31861 ttgaaaacta ccttttctta atgattaact acttgatttt caacccggtt tcaggtaaag
    31921 tatagctgtg tgctctaggc acatgacctg ccacttggtt aagcgaatca ccaacgtttt
    31981 actcagtcgc aggggaataa tcgaaagagg tgaaagaaat ggcaatttca aaagaacgca
    32041 agaatgaaat catcaacgaa tacgctcgcc atgaagggga tactggttct ccagaagtac
    32101 aaatcgctgt attaactgaa gaaatcaacc acttgaatga acatgcacgc gtgcacaaaa
    32161 aagaccatca ttcataccgc ggcctaatga aaaaagttgg tcaccgccgt aacttgttag
    32221 cttacttacg taaaactgac gttcaacgtt accgcgaatt gatcaaacgt ttaggcttgc
    32281 gtcgttaatt ttagtcaaaa agagtgggat tcacatgaat ctcacttttt ttactcgtat
    32341 atcaattaag taaaattccg tgatcaagct agatggactc ctactaacca aatgaaagct
    32401 gctcaaaaga cggctttcgg cagttttcat ttgtttagta gcgtcttgct ttcacgagaa
    32461 ggaaaaggag aagttatgtc agaaaaacaa gtattcaaaa cgacatgggg cggccgtccc
    32521 ttacaaatcg aagtcggtca gctagcaaaa caagcaaacg gagccgtttt agtccgttat
    32581 ggagatacag tagtcttaag tgcagctgtg gcctctaaag aagcaaaaga tacagacttt
    32641 ttcccattaa cgatcaatta tgaagaaaaa atgtatgcag cagggaaaat ccctggaggt
    32701 ttcatcaaac gagaaggccg tccaagtacg gaagcaacat tgactgcccg tttgatcgac
    32761 cgtccaattc gcccgatgtt tgcagaaggc ttccgtaatg aagtccaagt aacgaacatc
    32821 gtgatgagtg tagaaacaga ctgctcgcca gctatggcag caatgctagg ttcttcttta
    32881 gcactatcaa tctcagatat cccatttgac ggcccaatcg caggcgtaga agttggtcgt
    32941 gtaaatggcg agtacgtctt gaatccaaca gtagaacaag cagaacaaac agatatcgaa
    33001 ttgactgttg ccggaacgaa acaagccatc aatatggttg aaagcggcgc gaaagaagtt
    33061 tcagaagaag acatgcttgg tgcattgctg tttggttttg acgctatcaa agagttagtt
    33121 gctttccaag aagaaatcgt tcaagcagta gggaaagaaa aaatggaagt gactttactg
    33181 caagttgatg aagtattgaa aaaagaaatc tttgatgctt cttacgctac aatgaaagca
    33241 gcagtaatga ctgaagaaaa acttgctcgc gaagataata tcgagcaggt gaagatcgat
    33301 atccgcgaag cttatgctga gaaatttgca ggtcatgaag atgaagatca cttgttgaaa
    33361 gaagttaaac aaatcacaga agatcttgaa aaagatgtcg tacgtgaatt gatcaccatc
    33421 gataagatcc gtcctgacgg gcgtaaattg gatgaaatcc gtccattgtc atcagaggtc
    33481 agcttattgc cacgtgtaca tggatcaggt ttgttcacac gaggacaaac acaagcttta
    33541 tcagcttgta cactcgcacc tctaggagag caccaaatca ttgatggttt aggggtggaa
    33601 gtcagcaaac gattcatcca ccactacaat ttccctcaat tttctgttgg ttctacagga
    33661 cgtgcaggct ctccagggcg tcgggaaatc ggacatggcg cattaggtga acgtgcgtta
    33721 gcacaagtaa tcccttcaga agaagaattt ccttatacga tccgtttagt agcagaagta
    33781 ttggaatcaa atggttcttc ttcccaagca agtatctgtg ctggcacatt agcattgatg
    33841 gatgcaggtg taccaatcaa agcgccagtc gccggtattg cgatgggact tgtttctgat
    33901 ggtgaaaact atacgatcct aactgatatc caagggctgg aagatcatct tggcgatatg
    33961 gactttaaag ttgccggtac aaaagatggt atcactgctt tacagatgga tatcaagatt
    34021 caaggaatca cagaacaaat cctaactgaa gcattaactc aagcaaaaca agcacgtatg
    34081 gaaatcttag aagaattgac ttcaacgatt gctgctccac gtgaagaatt aagccaatat
    34141 gcaccaaaaa tcgaaatgat ccagatcgaa cctgctaaaa tcaaagatgt tatcggtaaa
    34201 ggcggcgata cgatcaacgg aatcatcgat gaaactggtg tgaaaatcga tatcgatcaa
    34261 gatggaaaag taagtattgc ttcagctgat gcagaaatga tcaaaaaagc tatcaagatc
    34321 atcgaagatt tgaccaaaga agtcaaagtt ggtgaagttt atctagggaa agttgtccgt
    34381 atcgaaaaat tcggtgcatt cgtcaacttg atcaaaggaa aagatggact tgtccatatt
    34441 tcccaattag caaacgatcg tgtcaataaa gtcgaagatg ttgttaaact aggtgatgag
    34501 atccttgtga aagtaactga aatcgataaa caaggacgcg taaatctgtc aagaaaagca
    34561 atgttgaacg aagatggttc aacgaaaaat gaagaaaaat aatttaattc ataggaataa
    34621 tgtacttgtg atcgtgaagg tgacaggggg acaataatta ttcgaaaaaa ggggactgtg
    34681 acaaaagccg tgtcacagtc cttttttttc gaataaacgg tgttcaaaag cttgcatctg
    34741 cggtaataag tccagccaag ttaaaaacat taggagctgt ttttatttgg ctgttcttat
    34801 tacttgatgc agaacagctt ttttacaacc tctccaataa acgatgttca atcagctgtc
    34861 cctcgatatt agctcaaaaa acggcaaaat atgaggaact atttttccgt ttgtttctct
    34921 aatactcagg tcaaaccgct gatttcacaa cctctgattc acggtgttcc agaagcagct
    34981 tctacggaaa taagctgaac acttttgtta caacttcttt ttttctggag tttcttggcg
    35041 catatttttg gagtccttct gaataaagcg taaaataaaa gtgagaaata tggataaagg
    35101 agggaaagga gatgctgaat tttttatggt cgttatttat cggcggtata ttaggtttga
    35161 tagctggcgc aattttagga aaggaagtcc cgggaggcgt atctggaaat atcattatcg
    35221 gttttttagg tagttggctc ggcgaatttt tactaggacc actgggaccg gaactcggcg
    35281 gtttttattt gataccagca ttattgggtg caattttttg ccttgctctt tattcatttg
    35341 tggcaagacg tatcagagaa cgtccaaaga aagaataatt ttattcaaat aagttgacac
    35401 ttcagtgtgt tacttggata attagaagca atttaggaaa gcgggtgtca tggataagaa
    35461 tatcatctat cattcaagct tacctaaagg atatccatat aacaagaaat gagggaaaaa
    35521 cgtggcaaca acgcacaata ccaaaaagat atcaatgact ggcctgcttg tagcgatggg
    35581 agtcgtctac ggagacatcg ggacaagtcc gctttacgtt atgaaatcga ttgttgaaga
    35641 taatggcgga ttgtcaggaa tcaatcctga ttttgttatc ggatcagtct cattgatttt
    35701 ttggacattg actctactga caacaatcaa atatgtcgtg attgcattga atgcagataa
    35761 ccatggtgaa ggtggaattt tctcactcta cacacttgtc cgaaaaggtg ggaaatattt
    35821 gatcattcct gcaatgatcg gaggagctgc cttactagca gatggggtat tgacccctgc
    35881 tgtgacggta acgactgcga ttgaaggctt gcgaggaatt ccgcagtttt ttgaccggtt
    35941 cggtaataac cagaacgtga tcgtcattat cactttagtt attatttttg ttttattcat
    36001 ggtccaacgc tttggtacag atattgtcgg aaaagcattc ggaccaatca tgttcgcttg
    36061 gtttactttt ttaggcgtaa tgggattaat gaacttcagt caagactgga cagttatccg
    36121 tgcactgaat ccttattatg ctatccagct gctttttagt gcagataaca aattaggctt
    36181 gttcattctt ggaaatattt tcctggctac gacaggtgca gaggcgcttt attccgatct
    36241 tggacatgtc ggcaaacgaa atattcgtct gagctggcct tacgtcaaaa tttgtttggt
    36301 tatcaattat tttgggcagg ctgcgtggtt gttgaatgtt tatcaaaacc ctgaagcaca
    36361 gcaaatcaag aacctgaatc cattcttcca aatgatgcct caaagttgga tcgtgatcgg
    36421 cgttgctttt gcaacagtcg ctgcagtcat tgcttcgcaa gcattgatta caggttcgtt
    36481 tacgctagtt tcagaagcaa tcaagttgaa actgctgcct cggttaaaga tcatgtatcc
    36541 tgggaacagt atcgggcaaa tgtatatccc agcagtcaat ctgattttat ggatcgcctg
    36601 ttctctagtg gttgtcacat tccgaacgtc tcatcatatg gaagctgctt acggtctatc
    36661 gatcactgtg acgatgttga tgacaacagc actgctttac ttttatctta tccaaaatgg
    36721 gcattctcgt tggcttgcat atttagttac ctttttcttc ggtgcgatag aagtcgtttt
    36781 ctttatctca agtgttgtca aattcttccg aggcggtttt gtagccgttc tgattggttt
    36841 gatcatctta gtcgtgatgt ttatttggga acaagggaac attattcgcg aatcgtcaac
    36901 cgaggatgtg tctttgagag attatatccc gcagcttaga gaattacgtg atgatacttc
    36961 tttaccaatc tatcagacaa atgtggtctt cttggttcct gatatggctg aagataaagt
    37021 tggccgacag ttcatctatt cgattttaga taaacggcct aaaagagcac gagtctattg
    37081 gtttgttcat gtggaagtga cggatgagcc atatacgaaa gaatatcaag tagatatgat
    37141 ggacactgat tttattgtcc aagtgaatct gttcttaggt ttcagggtcc aacaggaaat
    37201 caacgtatat gttcgccaag tcatccatga tttgatgaaa caaggccgtt tgccaaaaca
    37261 gccacaaaga tactctctga caccagggcg agaagttggc gatttccaat ttgtcctgat
    37321 tgatgaagta gtttccaatg tgacaactct aggaaaatgg caacgtcaaa tcatgcaggc
    37381 aaaactggca atcaaaaaaa tcgctacctc gccagaaaca tggtttgggc tagagtacag
    37441 cgaagtaaaa catgaaactg ttccgcttat cattggaaat actcggaaaa catggttgaa
    37501 agagcggttg tgaaagtagc gctttgacct gaactaatgt cgaatgtcag ctacttgaac
    37561 accgtttatc agaggtcatg aaaaagctgt actgcaacaa gcagtaagaa cgagcagata
    37621 aaaacagctt ctcatgtttt tcatctgttt gggcttaatg ccgtagatgc aagcttttga
    37681 ataccgtttt tatcagaggt catgaaaaag ctgtttagca acaagtaacg caatgatttt
    37741 gaagtgataa ggagatgtgg ctgaaaaatg ccgcatctcc ttttttattc ctaaatatga
    37801 atacgcatta ttttcaatct ttttgtagaa aaagggtaca atagaaaaag agaaaggcgg
    37861 gagaatatgg ttatcaattt cattatttta tttttgatga ttgcaattac ttcatttttt
    37921 gtagcaagtg aatttgcttt agtgaaaatc agacgatcaa gattggaaca actagaaaag
    37981 gaaaatgtaa agaacgccaa gctagctctt catgttacac accatttaga taactattta
    38041 tcagctagtc aattaggaat tacgctaacc ggtttgatca tcgggtgggt cggtgaagga
    38101 tctgtcgcgg ctttactaga accagtgatc ggtaaactac cttttagttc ggctattagc
    38161 agtacaatct ctgtggcgct gggatttatt ttagttacgt atatcgatgt ggttattggc
    38221 gaactgcttc ctaagagcta tagtatcgtc aatacggaga aggtcgtctt gttcgtagtt
    38281 aaaccacttc attattttta taaagtaatg tttcctttta tttgggtatt gaatcattca
    38341 gctgcagggc tagggaagct attaggtgtg cgtctcgtat ctgaaggaga agagacattg
    38401 tctcaagaag agctgacatt agtcgcattg aattcttatg aaaaaggaga gttgaccaaa
    38461 gaagaatatt attatttgga aaacgtgttt gaatttgatg ataccttggc aaaagatata
    38521 caagtagacc gtacgtccat gcaagtattc gaagcttcag aaaccgtcaa agaagcgata
    38581 aaagcttcga ttgaacaagg acatactcgc tatccggtta tcttagaatc aaaagacaat
    38641 gttttaggtt atgtcacctt gccagatttg atcaagcaat cttataaaga tgaccagctg
    38701 acagtagaac agttgattga agagccgatc gtaacaactg aaacgatccc tataaaaaaa
    38761 ttattgacga tcatgcgaaa aaagggtaag catattgcta tcttaaaaga tgaatacgga
    38821 gggacaagcg gcttagtgac gattgaagat attttagaag aaatcgtcgg ggaaatccga
    38881 gatgagacag atttggacga agcattgata gcggaacagt cagatggctc ttatatcatc
    38941 tccggcaaac taacattaga tgatttccag cgttattttc atgtagagat tccagagttt
    39001 gaagaaacga attttactac acttgcgggc tttgcttcta gccgatataa agaaataaaa
    39061 gcaggaacaa tcattgagat tgcatcattc cgtttcacgg tattagaata ccagcatgca
    39121 catatcgatt atttcaaagt agagtccacg gaaagaaaaa cagaataaaa gaaaaaaggc
    39181 tgtgacattt tttgtcacag cctttttgtt atctaaaaaa cctgttttcg gtaaacgaag
    39241 aagatatttt caataaaagt tttcattttt agccgcaacg atttctttta atcaaggcta
    39301 tcttgcttct gcatgttcta atgtgaaatg ttcctcttca cccatgtgtt tcaattgttt
    39361 catcgaacgc atataagagt aaagcaacac ggcaacagaa ccgatccaag ctaaaggaga
    39421 agctgctgct gctcctgggt atccccacat tcctgtaaga acgatagctg caaaagaacg
    39481 cataagcaat tccatcatac cggcgatcgt agggatcttg ctttgtccaa gcccttgaag
    39541 tgtgtagcgt aaaatgaaca ggactgctaa gatccagtaa agagacccat tgatattgaa
    39601 gtaggtttgt gctaattcaa atacacgtgt ttctgatggg ctaacaaaca attgaacaaa
    39661 gaaatgaccg aagaaaatga cgatagcacc tgctaagaga ctaaatccga cactcatcag
    39721 taaagtttga cgtacccctt gcaagattcg gccatattct ttcgcaccat agttttgtgc
    39781 agtgaaagtt gccattgcaa taccaaatga catcatcggc tgatttgcaa attggtcgat
    39841 acgaccagca gcggcttgag cagctacgac atctgtccct agactattta gtgctgcttg
    39901 taacacgata gcaccaacag cgatgatcga agattgaaac gccattggca aagcagcatt
    39961 taggtgtaca gcaaactctt ctttttcaaa tttgaaatct tttttgcgta actgtaacaa
    40021 aggcattttt ttcttgatat acactagaca cagtacgctg gaagaaactt gtgcgattac
    40081 tgtagcaata gctgcacctt ccacacccat gccaaaatag ataataaaga tcaaatcaag
    40141 tacgacgttg atgatgaccg caatgatcag aaagaatagt ggtgtgcggc tgtcaccaag
    40201 tgcgcgaatg acattagaaa gcaaattgaa actgactgat gcaaagatcc ctaataaaat
    40261 aattgaaata aatgtttgtg cttgatcaag tatttcagga ggagtttgca tcaattgaag
    40321 catcggacga ataaacagga gacttaggac agttaaaatc acagtgacga caagactaat
    40381 gacaacacta gcagcaaaac ttttcttcag accccgataa tcttttgcac cgtaacgttg
    40441 tgcggtaata atggctaaac cagctgttaa accttgggca aagccaatga tcaaaaatgt
    40501 caggcttcca gttgcaccaa ccgctgccag cgcattttta cctagcgtct gacccacgat
    40561 gatcatatcg atcatattgt agaactgctg aaaaacattt ccgatcagta aaggaacagt
    40621 gaacatcaaa atcagtttag cggggcttcc ttttgttaaa tcgcgcatct tttaaactcc
    40681 ttatatcttt aaaatttagt tagtagtata tagcgtttat cctgagaata ccaattagaa
    40741 atcaatgata atttatgaaa ctccataaaa agaagccaag aattgagtca aagaatcaac
    40801 aacttggcct ggtctgtaaa ataagggaca tccacgttta tctatcacct taatattgtc
    40861 taatctcact tggtaaaagg atcgtttctc tcgtaccgtc atcataggcg tatacttgag
    40921 caggaaattg gttcgaatag gcaaacggca aatcaatatc catttccaca ttgcttcctg
    40981 cttgagaaaa atgcatcgtc acttttccac gattttcgat caaatcaaag gttaatatat
    41041 tttctaatgg aatgactccc ttaagatcta ggtcaataat aaaccatata ctgtcaatca
    41101 attcatctgg caaacttgat acgacaccaa ctgaagcgta acggttgcgg ctgctatcaa
    41161 aactttcaaa catctgttta acctccttcg aatcaacgaa tgttttattt t
//
LOCUS       DANKAZ010000016        40414 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000016 DANKAZ010000000
VERSION     DANKAZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="PWY67_000789"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="PWY67_000789"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL2057096.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="PWY67_000790"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="PWY67_000790"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL2057097.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="PWY67_000791"
     CDS             complement(2303..2965)
                     /locus_tag="PWY67_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2057098.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="PWY67_000792"
     CDS             3149..4795
                     /locus_tag="PWY67_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2057099.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="PWY67_000793"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="PWY67_000793"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2057100.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="PWY67_000794"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="PWY67_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="PWY67_000795"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="PWY67_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="PWY67_000796"
     CDS             complement(8944..10023)
                     /locus_tag="PWY67_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2057101.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="PWY67_000797"
     CDS             complement(10126..10566)
                     /locus_tag="PWY67_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2057102.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="PWY67_000798"
     CDS             complement(10623..11033)
                     /locus_tag="PWY67_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2057103.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="PWY67_000799"
     CDS             complement(11213..11647)
                     /locus_tag="PWY67_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2057104.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="PWY67_000800"
     CDS             complement(11747..12595)
                     /locus_tag="PWY67_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2057105.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="PWY67_000801"
     CDS             12984..14750
                     /locus_tag="PWY67_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL2057106.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="PWY67_000802"
     CDS             14852..16111
                     /locus_tag="PWY67_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2057107.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="PWY67_000803"
     CDS             16258..17559
                     /locus_tag="PWY67_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2057108.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="PWY67_000804"
     CDS             17562..18413
                     /locus_tag="PWY67_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2057109.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="PWY67_000805"
     CDS             18426..19241
                     /locus_tag="PWY67_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL2057110.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19392..20558
                     /locus_tag="PWY67_000806"
     CDS             19392..20558
                     /locus_tag="PWY67_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2057111.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20788..21435
                     /locus_tag="PWY67_000807"
     CDS             20788..21435
                     /locus_tag="PWY67_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2057112.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21519..22463
                     /locus_tag="PWY67_000808"
     CDS             21519..22463
                     /locus_tag="PWY67_000808"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL2057113.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22598..24049
                     /locus_tag="PWY67_000809"
     CDS             22598..24049
                     /locus_tag="PWY67_000809"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL2057114.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24070..24906
                     /locus_tag="PWY67_000810"
     CDS             24070..24906
                     /locus_tag="PWY67_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2057115.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24906..25565
                     /locus_tag="PWY67_000811"
     CDS             24906..25565
                     /locus_tag="PWY67_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2057116.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            25691..26380
                     /locus_tag="PWY67_000812"
     CDS             25691..26380
                     /locus_tag="PWY67_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057117.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26386..27342
                     /locus_tag="PWY67_000813"
     CDS             26386..27342
                     /locus_tag="PWY67_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL2057118.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27386..27898
                     /locus_tag="PWY67_000814"
     CDS             27386..27898
                     /locus_tag="PWY67_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2057119.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28154..28702)
                     /locus_tag="PWY67_000815"
     CDS             complement(28154..28702)
                     /locus_tag="PWY67_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2057120.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28791..29972)
                     /locus_tag="PWY67_000816"
     CDS             complement(28791..29972)
                     /locus_tag="PWY67_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL2057121.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30208..31716)
                     /locus_tag="PWY67_000817"
     CDS             complement(30208..31716)
                     /locus_tag="PWY67_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL2057122.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31875..32066
                     /locus_tag="PWY67_000818"
     CDS             31875..32066
                     /locus_tag="PWY67_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057123.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32269..33186
                     /locus_tag="PWY67_000819"
     CDS             32269..33186
                     /locus_tag="PWY67_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL2057124.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33179..34252
                     /locus_tag="PWY67_000820"
     CDS             33179..34252
                     /locus_tag="PWY67_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057125.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34400..34918
                     /locus_tag="PWY67_000821"
     CDS             34400..34918
                     /locus_tag="PWY67_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057126.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34899..35291
                     /locus_tag="PWY67_000822"
     CDS             34899..35291
                     /locus_tag="PWY67_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL2057127.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35367..36302
                     /locus_tag="PWY67_000823"
     CDS             35367..36302
                     /locus_tag="PWY67_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL2057128.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            36435..37214
                     /locus_tag="PWY67_000824"
     CDS             36435..37214
                     /locus_tag="PWY67_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2057129.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            37207..37524
                     /locus_tag="PWY67_000825"
     CDS             37207..37524
                     /locus_tag="PWY67_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2057130.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37768..39378
                     /locus_tag="PWY67_000826"
     CDS             37768..39378
                     /locus_tag="PWY67_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2057131.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            39535..40383
                     /locus_tag="PWY67_000827"
     CDS             39535..40383
                     /locus_tag="PWY67_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2057132.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13231 a         7260 c         7799 g        12124 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    19321 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    19381 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    19441 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    19501 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    19561 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    19621 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    19681 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    19741 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    19801 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    19861 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    19921 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    19981 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    20041 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    20101 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    20161 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    20221 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    20281 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    20341 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    20401 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    20461 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    20521 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    20581 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    20641 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    20701 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    20761 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    20821 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    20881 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    20941 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    21001 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    21061 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    21121 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    21181 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    21241 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    21301 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    21361 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    21421 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    21481 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    21541 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    21601 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    21661 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    21721 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    21781 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    21841 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    21901 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    21961 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    22021 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    22081 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    22141 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    22201 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    22261 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    22321 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    22381 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    22441 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    22501 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    22561 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    22621 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    22681 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    22741 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    22801 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    22861 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    22921 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    22981 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    23041 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    23101 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    23161 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    23221 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    23281 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    23341 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    23401 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    23461 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    23521 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    23581 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    23641 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    23701 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    23761 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    23821 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    23881 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    23941 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    24001 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    24061 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    24121 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    24181 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    24241 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    24301 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    24361 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    24421 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    24481 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    24541 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    24601 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    24661 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    24721 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    24781 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    24841 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    24901 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    24961 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    25021 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    25081 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    25141 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    25201 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    25261 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    25321 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    25381 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    25441 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    25501 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    25561 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    25621 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    25681 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    25741 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    25801 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    25861 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    25921 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    25981 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    26041 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    26101 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    26161 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    26221 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    26281 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    26341 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    26401 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    26461 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    26521 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    26581 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    26641 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    26701 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    26761 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    26821 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    26881 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    26941 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    27001 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    27061 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    27121 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    27181 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    27241 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    27301 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    27361 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    27421 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    27481 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    27541 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    27601 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    27661 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    27721 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    27781 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    27841 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    27901 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    27961 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    28021 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    28081 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    28141 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    28201 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    28261 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    28321 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    28381 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    28441 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    28501 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    28561 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    28621 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    28681 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    28741 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    28801 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    28861 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    28921 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    28981 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    29041 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    29101 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    29161 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    29221 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    29281 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    29341 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    29401 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    29461 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    29521 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    29581 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    29641 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    29701 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    29761 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    29821 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    29881 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    29941 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    30001 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    30061 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    30121 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    30181 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    30241 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    30301 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    30361 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    30421 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    30481 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    30541 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    30601 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    30661 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    30721 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    30781 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    30841 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    30901 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    30961 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    31021 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    31081 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    31141 atgatacagt tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac
    31201 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    31261 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    31321 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    31381 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    31441 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    31501 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    31561 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa
    31621 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    31681 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    31741 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    31801 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    31861 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    31921 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    31981 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    32041 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    32101 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    32161 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    32221 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    32281 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca
    32341 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    32401 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    32461 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    32521 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    32581 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    32641 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    32701 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    32761 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    32821 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    32881 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    32941 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    33001 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    33061 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    33121 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    33181 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    33241 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    33301 tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac
    33361 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    33421 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    33481 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    33541 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    33601 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    33661 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    33721 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    33781 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    33841 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    33901 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    33961 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    34021 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    34081 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    34141 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    34201 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    34261 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    34321 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    34381 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    34441 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    34501 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    34561 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    34621 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    34681 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    34741 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    34801 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    34861 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    34921 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    34981 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    35041 aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga
    35101 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    35161 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    35221 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    35281 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    35341 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    35401 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    35461 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    35521 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    35581 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    35641 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    35701 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    35761 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    35821 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    35881 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    35941 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    36001 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    36061 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    36121 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    36181 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    36241 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    36301 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    36361 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    36421 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    36481 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    36541 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    36601 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    36661 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    36721 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    36781 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    36841 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    36901 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    36961 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    37021 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    37081 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    37141 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    37201 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    37261 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    37321 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    37381 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    37441 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    37501 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    37561 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    37621 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    37681 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    37741 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    37801 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    37861 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    37921 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    37981 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    38041 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    38101 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    38161 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    38221 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    38281 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    38341 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    38401 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    38461 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    38521 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    38581 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    38641 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    38701 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    38761 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    38821 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    38881 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    38941 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    39001 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    39061 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    39121 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    39181 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    39241 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    39301 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    39361 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    39421 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    39481 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    39541 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    39601 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    39661 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    39721 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    39781 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    39841 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    39901 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    39961 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
    40021 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    40081 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    40141 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    40201 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    40261 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    40321 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    40381 taattccttt tattcaaaaa acataccaaa aata
//
LOCUS       DANKAZ010000017        39937 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000017 DANKAZ010000000
VERSION     DANKAZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PWY67_000828"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PWY67_000828"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL2057133.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PWY67_000829"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PWY67_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL2057134.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PWY67_000830"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PWY67_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL2057135.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PWY67_000831"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PWY67_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL2057136.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PWY67_000832"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PWY67_000832"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL2057137.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PWY67_000833"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PWY67_000833"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL2057138.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="PWY67_000834"
     CDS             complement(8053..8523)
                     /locus_tag="PWY67_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL2057139.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="PWY67_000835"
     CDS             complement(8569..8994)
                     /locus_tag="PWY67_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL2057140.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="PWY67_000836"
     CDS             complement(9061..9330)
                     /locus_tag="PWY67_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL2057141.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="PWY67_000837"
     CDS             complement(9341..10936)
                     /locus_tag="PWY67_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2057142.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PWY67_000838"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PWY67_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL2057143.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="PWY67_000839"
     CDS             complement(14489..15049)
                     /locus_tag="PWY67_000839"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL2057144.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="PWY67_000840"
     CDS             15402..16376
                     /locus_tag="PWY67_000840"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2057145.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="PWY67_000841"
     CDS             16546..17946
                     /locus_tag="PWY67_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL2057146.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="PWY67_000842"
     CDS             complement(18024..18746)
                     /locus_tag="PWY67_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2057147.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="PWY67_000843"
     CDS             complement(18825..19331)
                     /locus_tag="PWY67_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2057148.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="PWY67_000844"
     CDS             19454..21253
                     /locus_tag="PWY67_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL2057149.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="PWY67_000845"
     CDS             complement(21321..22895)
                     /locus_tag="PWY67_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2057150.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="PWY67_000846"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="PWY67_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL2057151.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="PWY67_000847"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="PWY67_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL2057152.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="PWY67_000848"
     CDS             complement(24672..25310)
                     /locus_tag="PWY67_000848"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL2057153.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="PWY67_000849"
     CDS             complement(25311..25961)
                     /locus_tag="PWY67_000849"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL2057154.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="PWY67_000850"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="PWY67_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL2057155.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="PWY67_000851"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="PWY67_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL2057156.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="PWY67_000852"
     CDS             complement(29104..29844)
                     /locus_tag="PWY67_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL2057157.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="PWY67_000853"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="PWY67_000853"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL2057158.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="PWY67_000854"
     CDS             complement(31215..32153)
                     /locus_tag="PWY67_000854"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL2057159.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="PWY67_000855"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="PWY67_000855"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2057160.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="PWY67_000856"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="PWY67_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL2057161.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="PWY67_000857"
     CDS             complement(35313..35618)
                     /locus_tag="PWY67_000857"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2057162.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="PWY67_000858"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="PWY67_000858"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL2057163.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="PWY67_000859"
     CDS             complement(36724..37008)
                     /locus_tag="PWY67_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057164.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="PWY67_000860"
     CDS             complement(37026..37916)
                     /locus_tag="PWY67_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL2057165.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="PWY67_000861"
     CDS             complement(37936..38154)
                     /locus_tag="PWY67_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL2057166.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="PWY67_000862"
     CDS             complement(38124..39824)
                     /locus_tag="PWY67_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL2057167.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
BASE COUNT        10796 a         8707 c         7253 g        13181 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaa
//
LOCUS       DANKAZ010000018        37273 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000018 DANKAZ010000000
VERSION     DANKAZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            25..702
                     /locus_tag="PWY67_000863"
     CDS             25..702
                     /locus_tag="PWY67_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2057168.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            705..1832
                     /locus_tag="PWY67_000864"
     CDS             705..1832
                     /locus_tag="PWY67_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057169.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(2032..2472)
                     /locus_tag="PWY67_000865"
     CDS             complement(2032..2472)
                     /locus_tag="PWY67_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2057170.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(2483..4516)
                     /locus_tag="PWY67_000866"
     CDS             complement(2483..4516)
                     /locus_tag="PWY67_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2057171.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(4518..6221)
                     /locus_tag="PWY67_000867"
     CDS             complement(4518..6221)
                     /locus_tag="PWY67_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2057172.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(6315..7265)
                     /gene="manA"
                     /locus_tag="PWY67_000868"
     CDS             complement(6315..7265)
                     /gene="manA"
                     /locus_tag="PWY67_000868"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL2057173.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(7304..8329)
                     /locus_tag="PWY67_000869"
     CDS             complement(7304..8329)
                     /locus_tag="PWY67_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2057174.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(8538..9407)
                     /locus_tag="PWY67_000870"
     CDS             complement(8538..9407)
                     /locus_tag="PWY67_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL2057175.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(9866..11758)
                     /locus_tag="PWY67_000871"
     CDS             complement(9866..11758)
                     /locus_tag="PWY67_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2057176.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(11755..13482)
                     /locus_tag="PWY67_000872"
     CDS             complement(11755..13482)
                     /locus_tag="PWY67_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2057177.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(13498..14148)
                     /locus_tag="PWY67_000873"
     CDS             complement(13498..14148)
                     /locus_tag="PWY67_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2057178.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            14352..14648
                     /locus_tag="PWY67_000874"
     CDS             14352..14648
                     /locus_tag="PWY67_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2057179.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            14820..15440
                     /locus_tag="PWY67_000875"
     CDS             14820..15440
                     /locus_tag="PWY67_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL2057180.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(15532..16698)
                     /locus_tag="PWY67_000876"
     CDS             complement(15532..16698)
                     /locus_tag="PWY67_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2057181.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(16710..17063)
                     /locus_tag="PWY67_000877"
     CDS             complement(16710..17063)
                     /locus_tag="PWY67_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2057182.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            17191..18213
                     /locus_tag="PWY67_000878"
     CDS             17191..18213
                     /locus_tag="PWY67_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL2057183.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            18232..18393
                     /locus_tag="PWY67_000879"
     CDS             18232..18393
                     /locus_tag="PWY67_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057184.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(18652..19086)
                     /locus_tag="PWY67_000880"
     CDS             complement(18652..19086)
                     /locus_tag="PWY67_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057185.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(19196..21055)
                     /locus_tag="PWY67_000881"
     CDS             complement(19196..21055)
                     /locus_tag="PWY67_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2057186.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(21173..22333)
                     /locus_tag="PWY67_000882"
     CDS             complement(21173..22333)
                     /locus_tag="PWY67_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDL2057187.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(22640..24064)
                     /gene="araA"
                     /locus_tag="PWY67_000883"
     CDS             complement(22640..24064)
                     /gene="araA"
                     /locus_tag="PWY67_000883"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL2057188.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(24080..24778)
                     /locus_tag="PWY67_000884"
     CDS             complement(24080..24778)
                     /locus_tag="PWY67_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2057189.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(24735..26396)
                     /locus_tag="PWY67_000885"
     CDS             complement(24735..26396)
                     /locus_tag="PWY67_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL2057190.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            26614..28014
                     /locus_tag="PWY67_000886"
     CDS             26614..28014
                     /locus_tag="PWY67_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL2057191.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(28266..29345)
                     /locus_tag="PWY67_000887"
     CDS             complement(28266..29345)
                     /locus_tag="PWY67_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2057192.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(29431..30390)
                     /locus_tag="PWY67_000888"
     CDS             complement(29431..30390)
                     /locus_tag="PWY67_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2057193.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(30402..31913)
                     /locus_tag="PWY67_000889"
     CDS             complement(30402..31913)
                     /locus_tag="PWY67_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2057194.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(31935..32498)
                     /locus_tag="PWY67_000890"
     CDS             complement(31935..32498)
                     /locus_tag="PWY67_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL2057195.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(32504..33367)
                     /locus_tag="PWY67_000891"
     CDS             complement(32504..33367)
                     /locus_tag="PWY67_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2057196.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(33381..34277)
                     /locus_tag="PWY67_000892"
     CDS             complement(33381..34277)
                     /locus_tag="PWY67_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2057197.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(34452..35741)
                     /locus_tag="PWY67_000893"
     CDS             complement(34452..35741)
                     /locus_tag="PWY67_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2057198.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(35713..37230)
                     /locus_tag="PWY67_000894"
     CDS             complement(35713..37230)
                     /locus_tag="PWY67_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2057199.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        10797 a         7803 c         6449 g        12224 t
ORIGIN      
        1 aaattttata ttggaggcct atctatgaca ttactgatca aaaacatgaa catgaaacgt
       61 ggtaaaaaac gtgtgattcg acaattgaat cttttagtag aatcaggaga agtagctgct
      121 ttgattgccc cgaacggatt tggcaaaaca actttgctga atggcattgc ccaactgctg
      181 ccaacaactt atgatcaatt gtcccttcac aacatctctc ctactgctta tacagattat
      241 cgccggtctt tcttttttct agaatcaacg aatcagttat ccgactatct atcggctgtt
      301 gattatttga ctttgatcaa atactattgg cagagcaaag tagaatgcca aactgttttc
      361 caacgattga agatgactga gtaccttact ttgccgattg gaaaaatgtc tctaggaatg
      421 aaacaacatg tcttgcttgc tgcttacttg atcagtgatt catctgtcat gttattcgat
      481 gaaccgttga acggattaga cccaggaagt attgatatct tgaatagcct catcagacaa
      541 tggcagaaag aggggaagac gattcttctt tcttcccacg ctatcgcgaa tatccaatcg
      601 atttgttcta aagcatgttt tctaaaaaat ggagaaatcg tcacagaatc ctcaaatatg
      661 gaagagattt tagctattta tcgtaaatta tatgaggtgt aactatgtct ttacttacat
      721 ttgaacgcaa tctcttacta aaaaacaaag taaacttttt atttcctcta ttgttggtcg
      781 tgctctttgc attcccactt ttttttgatc ataaacttgc ttatacagaa ttcgatgaac
      841 tgaatcataa ctatgaagag atgcaacgtt tgattgaaac gttgaaagaa gatgagaatg
      901 agaaagaatt tgtggaaagt ttagagaaaa gcaacaaact gatcgaagca atcttacatg
      961 caaaaaacac agggaatgtc cagcaaatgg tcgaagcaac ttatcattat gaaaaagata
     1021 tactggatcg actgatttct ggtcaaagac aaggaattcc aatcattgaa caacaaaaac
     1081 gtgtcgagtt gctccgttat atgaaagaac atcagatcca aagatattct atttttgact
     1141 tgccagcgca tctcccttta gcaaattact acgaaaatat tttttcagga atgatttcta
     1201 gttttttgat tttatgtata accgccttgt ttctttcttc catcatctct tatgaaaaaa
     1261 gaaagcaggt aatctctcta gtcaatctat taccggatag tatggtaaag aaacatagca
     1321 tccgttttac catttattat ggtgcagcta tgctaagtct cgtgatgcca tttttgatcg
     1381 tctctatact agtaataatc aaaaatggtt tgggagactt ccgttatcct gtaggaacga
     1441 tcattggtca ggagatacga atccttccga tgtatgagta tcttttccag agctttctgt
     1501 tcctgttgtt gtgggtgctc tttttaagta caatcagttt tcttctatct gctttatttg
     1561 aacattctct agtcaatctt ttaggtacac ttctttgtct tttcttagca gagtatcgct
     1621 tgttttcttc gattggatgg atagaatcaa tcagtcatta cttgccaact tcttatgttg
     1681 attttcaaaa tgtgatcata ggaggagata ttttctcgcc actagcttca gaacaggtta
     1741 ctttcatgaa tggtatcttg acacttggta tatggagtat cgttttatta ttcataggta
     1801 tgggcactat ctatataaaa aagagctatt aaaacacaaa aataataggg gcggtgacaa
     1861 aaaccttgtc accgtcccct atttcaaata aacggttttc tagaataaat agttttcaaa
     1921 agcttgcatc cgcagtaata agaacctccc aacaaaaata gctcctctta tttttatctt
     1981 ggttgaagtt attacttgat gcaaggcagc tttttttact gtgaacattg gttatatatg
     2041 tccatgttca aaaatcattt ggacttcttc aatggttttc gcatcactga gcgccattac
     2101 ttggtcgatg tctgaacaga aaaaggctaa atcttggagc accgctaact gatgttcttt
     2161 ttttaaagcc actacaaata agagtgtcgc gatttttggt tcctcttttg ttccaaagtc
     2221 cacaccatca ggcacttgga ctaaatagat tccttcttct actacttctt catctttgtc
     2281 agtcccatgt ggcaaggcta cgaaattccc gatatagaca ctcgcttttt cttgtctttc
     2341 gatcattgat tggatatatg ccggtttgat tcccccttgt tgggtcatcc actggccgat
     2401 ctcggtcaac gcttctttcc aattttgata ggattgatcg agcaatattt tttctgctgg
     2461 tagctgtatc atcttttcac cttcatttct ttatttcttc aatcaggatt ttcgctaaca
     2521 actgataaac gatttcttta tttcctgagc ggaagatctc ggtgttcaag tcattcatga
     2581 tgattgctcc actgatcttt cctaaaagaa tcccgtcttt ttcttggatt ggcgaaggcg
     2641 ccagcataac taacatacgg gacaaatcga ctggctgtct atccatccct tcaatggtca
     2701 acggatgttt caaattgaaa atacaaaaga tcggtttaga aatcaatgag ctggatgtat
     2761 ggaataaccc catatttgta tgaggaatac cgataggtgc ttgttcatat cgcttaagca
     2821 gccggcgatg aaccacctct tcatccgtta ctagtccttc aggaaaataa ggaaataagc
     2881 tgcgaatcgt ttcggaaatc gtctcaggat tatccagttc ttggataaaa aaacgaccga
     2941 gtaattgatt gatttgatcc atgaaatgca ttgtttcctc ataagtatct tctatcatcc
     3001 actcgttatt gctgttatac gtaatttttt gccttttttt cttctcaata tgcgaaaaag
     3061 cttcttttag ctgcttgact tcatcttcta gcagtagcgg ggagactaaa atatattttc
     3121 caccgtatcc tggtaaaata gatgtagaaa taacaacatc atattcttct tgtaaatcga
     3181 ttttttttaa atcagcaatc cgatagaaat gaatttgttg gatgaaggga aaataccttt
     3241 tcaatcgtag ttccaacatg ctagtagaag ctaatccact tggcgaaaat cctgcaatac
     3301 tgatattgct ttctttcgga cttcgttcca atgaattcgc aaaatgaagg accatataag
     3361 cgatctcttc ttcggataat tttttttctt gaaaaatccg ctctactgct ttacggattg
     3421 cattcgcaac ttcctcgtat tgaaccatga ttttttctaa aatcggattg gtcagtgtct
     3481 cttcttgtaa aacggctctt gaaaacacac cagataaatg agtcaacagc attttgtaca
     3541 aagtttcatc ttcataaaaa tggacattcg tctcttcact tacttcttga atcagttgtt
     3601 ttactaaata agcaagctgt gtatcgaacg tctcttcaaa gaaattctga tcaaatgagt
     3661 tcgtgaagtc atttaataat cctgcaaaaa agacgatctc atttactgga taaagttgtt
     3721 ttgtctcaga agcgatccgt gaaaacaatc gtttagctgt ctgtagtaat tctttgtttg
     3781 ccagactatt atatgtctca tttgtcagta aatgattttt agtcacacgc tccatcgtca
     3841 atgtcagcat caaaaccaaa tgttcaagct ttctatcact tagattggtc tttttttgga
     3901 tcaattcttc cccgattact ttgcgtgcaa gagatagatg ttgcggatca acgaatcgaa
     3961 aaaaataatt cagtgatgca tcgaaagaaa tactatgaaa aagttcatat tcattgattt
     4021 ctaattctaa tacattcgct gtcagaagcc ggcggtattt ttccggtcct tcgatctcgt
     4081 atccctggtt ccgaatgatt tccaaaggca gttggcgaat actttcttcc aactgtttga
     4141 tatctgcata aaaagtggta ttactgatgc gatatgattc catgaaatga gcaagactca
     4201 acggctctgt cgtcagtaac aactcggcta aaatcgcgtg ttgtcgttta gcagtggata
     4261 agtcattact tttcccatct gtattttctt ttaattgctc taatacttct tctgatgcct
     4321 caatgacaaa tgagcctcta gctgcttttt tcaaagaagc atttaccgat ttcaatgaga
     4381 cttcaagatt atctaattcg cggtaaatag tccgtttact gaccttcaat aaattcatca
     4441 ttttttggat agatacaggt gacgggctac tgagaaattc tgctaataat tgtttttctc
     4501 tgggcgataa atacatctta tatttctccc ttcacgagtt tttcgtattc attcgtagct
     4561 acccaatgtt caactagaac tagccgaggg atcttttctt ctagctgttc agttacagct
     4621 agttctcttt gtgcgatata aatcgtttct tcttggattt tgattttttc tattgattct
     4681 tttgagataa caaactgttt tttcgctttt tttgctaatt gacttaaaat cgtcatgccc
     4741 atagtttgtg agccgcgtct gttgttttta tacaaaaata caatattagc aggcgacttt
     4801 ttttgttctg tgatatctgc tatctctttt ttttcttccc gctttggaaa ttggatcgct
     4861 tcttttattc gtgacagcaa gatatctgtt tccaaaaagt gatccagtac atagatttga
     4921 ctttgaggta ctttttctct tgcaagaccc gctagttcat gttgcacgat aaccagctga
     4981 tttacttggt tattcaattc atagatagag ctgtatccga cagaaagatc gattttttct
     5041 gctgctaatt tctttttcag cagggcggct cccatagcac ttgatcccat acctgagtcg
     5101 caagcaaaaa tgacggaatg aatgatagat ggttccaaga tttctcccct ttctttgacc
     5161 gttttgttct ctttttcttt atttggtaat ttcaatagta tcgctgcaca aatgaaggtc
     5221 acaaatgtac tgattgctat ccctatactt accccagcta gttgtggata aggcgcattg
     5281 gcaaaaatca cgagtagtga cccaggcgat acaggtgcag ctaatcccgt atctaaccat
     5341 tgaaatatca acgtgccgct tgccccacca gccattacag ctaaaaatag atacggtcgc
     5401 agcaagacaa atggaaaata gatttcgtga atcccgccaa tcgcatggat catcaatgct
     5461 ccactcgcat ttactttttc ctgtttttct gcaaaacaaa ggatagaaag taagatccct
     5521 aaaccaggtc caggatttgc ctccatcaaa aataaggaag agtacccgac tgtttgtgag
     5581 aattcgattc ctaaaggtgt caatacacca tgattgattg cattattgaa aaatagtact
     5641 tttagaggtt ctaagatcag actgacaagt ggtaacagat tcattgtgac tagccaagac
     5701 acaacggagg cgagcaacaa gtgaacacta tttaaaactg gttcaataaa aacgattcca
     5761 attatacaaa taacgctacc agtcagtcct actagtaggt tgctgacaag catttcatat
     5821 ccagccttga atttcacgtg gaatctagaa acaaattgtt catatacgat gccggcaagc
     5881 ggcccgataa tcatcgctcc tacgatttgt ggagcattgg aacgagcgat caaccctaaa
     5941 gaagcaattg caccggcgac tgcccctttt tgtcccccta ccgatttccc gcctgaataa
     6001 ctgatcaaga tgggtaaaag cagctggatc atccaatggc atacctcttc taatgtggtg
     6061 tgcaaatcgc cagacacacc ttttgttgct aatgacaaga ttccccaagc gataaacaga
     6121 cttagatttg gcatgatcat ttcatttaag tgatgtccag cttttctcag ttgtttcata
     6181 gacacgcgac tgctgttgtt tgctttccat ttgtattcca tctttttcac catacttcta
     6241 tttattgatg gacggatttt atgtagaaaa agcagcaaac gaaaaaccgt ctgctgcttt
     6301 cagctatctg ggaactattt gcctggttca gtcgcaatga ttgctagttc accttggacc
     6361 gcccactcag aaacgccatt tggaataata cagcttgttc ctttttctaa tggatatgtt
     6421 tttccgtcaa cgacaaggtc tcctgctcct tcgattaccg tcatcaatgt ataaggggct
     6481 tgttttttga aagatgtgat tcctttaata tcccactcat atacattgaa aaactcagtt
     6541 tctaaataag tgacgatcga tgaattccct tttcggactt ctttgatttg taattctggt
     6601 gttttcgcag ggacagttgt tacatcgatc gattgttgga tatgcagctc tcttgtttgt
     6661 ccttgcgcat cttttctgtc gtagtcatac acgcggtaag tcgtgtcaga actttgctgt
     6721 gtttctaaaa tcatgattcc tttaccaata gcatggatcg ttccgcttgg tacataaaag
     6781 aaatcacctt ttttaactgg tactttcttc aaaaggtcat cccagcgccc atctttgatc
     6841 atctcagcta gttcttctct tgtctttgca tgatgaccat agatgatttc tgcaccaggt
     6901 tcagcatcga tgatatacca gcattcagtt tttcctaatt caccttcatg tttcatccca
     6961 taggcatcat ctggatgtac ttgtacagat agatcatctt ctgcgtctaa gatcttgatc
     7021 aataatggaa acactggttc tgaaggatgg ccaaacaatt ccttgtgttc tgaccatagt
     7081 tcatctaatc ttttcccttt gaattcgcca ttttctacta cactgacacc atgaggatgc
     7141 gcactgatcg cccaatcttc tccgatttta tcgttaggga tatcaaaacc aaagactgaa
     7201 cgtaaacgac ttcctcccca aattttttct tgaaaaactg gttttaaaaa cataggttcc
     7261 tgcataattc aaactcctct catttttcat agctattata gcattatttc ggtaataccg
     7321 ctttctcttt caaaaagttt tcaacaattt catctcgaca tatgagatac tccgcacgat
     7381 gatcgacagg atgtacgcga tttgacaaaa atatcaacgc ttcttgtgtt tttatatcaa
     7441 tcaagaaaaa cgtacctgta taaccagtat gaaaaagaat cgcatgaccg tcttctacat
     7501 ccttttttaa gtcccatcct aaagaacgat agccattttt tttcggtgtt tgatcttgca
     7561 gtaaagaatc aatcgtctct tcttctaaaa aacgaccatt acggattttt cccttgttca
     7621 aatacatttg gacaaaagta atgctgtctt tcaaatcaat aaagagaccc gcgttacctg
     7681 catgttcagc caaaactctt gctttcggat catgagtttg tccacgcaaa acctcacccg
     7741 aaggcagttg ttctgtggga acgatttttt tggaaggaaa aggaagaaat aagctgttca
     7801 tcattcctaa tggttccaat acttgttctt gaaagatttc cactgcagtt tcttggtaaa
     7861 tttcctctag catcaaccca agaagaatcg taccggcatc tgtatatttt acttcttttc
     7921 ctagttgctc acctggttct agcttcaaat aagcggcgat caattcttcc tgagatagct
     7981 tatctctgtt tggtatccat gttttgatat cagaagtatg tgtcagtaaa tgacgcaaag
     8041 tgattgtctg attttcaaat tcaggaagat atttatgaag cggttgatcg agttctatct
     8101 ttccatcttc taatagacgt aaaactaatg aagtggttgc aacgactttc gtcagtgagg
     8161 ctgcatcaaa caacagatct tctgtcatca gctgcttttc aggaacgact tgggcaaagc
     8221 cggctgtaaa tgtttctact tgattttctt ttatgaaggc gactgctaca cctggataaa
     8281 atccttcttc catctttttc aaaataagct gtttcgtgtg tggatacatc tttttctcct
     8341 gcttttctat cagattctta cagtccaagc ttaaagctta atctatgagg aatcaacctt
     8401 ttttacacaa aaaaactgcg cgaaaaaccg cgcagtttcc cccggtatac tattttctct
     8461 ttctatctct agaataagcg atagtatgac cgttcataaa atgacgagac atgcgacaat
     8521 cgttaagaaa gaaaaagtta agcgtcgcat cgaatgtcag ttttattctt ttcttttggc
     8581 tcactgacga accaccattc gattcctata gcatcataga ttgtgagtac tgtgtgcttt
     8641 ttgtctaaga agaaatcaac tgatagttgc tgcaagtgag caatcagtcc ttccatgctt
     8701 tcagtatcga tcatgaattt cagaaaatca agtccaatca cttcatctgt aggcaaatca
     8761 attgcgcctc cgctagcttc tgtcaaacca acatggaaat ctccttcatt caatacatgg
     8821 atgccctctt cttcattatg taccttcaaa ccaagtacat gattaagaaa ttggcgttct
     8881 tttgttaagt tagtaacatt taaatggatt ttttcaaaat aagaacctgt tgatagttta
     8941 ctgaattttt cttcagattt tcttaataat gcttcttgat ccattgtttc tggaatttct
     9001 ctttggtcgt tcttattctc ttcatggtaa atttccaatt cattctcttc tgggtctacg
     9061 agcaaaatcc ccatacgtcc atgatcttct aaagcttctt tgattggata attcatttta
     9121 cggatacgac aaaggatatc tgccatttct tctgtgctag ggataatcaa tgaaaaacgg
     9181 ttcagctttt tgatcacccc catgtgatca ttcgctctag gactttcttc cagcagcaac
     9241 atttcacttc cagcttcttc tgttcctaaa atggctaatt cgttttcttc tcttttcaaa
     9301 tcaaagccaa caacgttctt gtaaaaatca atcattttat ctcggtcttt cacccgaatg
     9361 gctacacttt ttaaataggt ggcagccccc aattgaaaat ctttcatatt tcttgctccc
     9421 ctctacgtca agctttattt taacgatgga ttcttcttct ttcaagcaat aactttgatt
     9481 ttgttaaaaa aaatgtgaga aaattcgcat tttgcttgtt ttttgtaaat taatgagtaa
     9541 aaactcttat aaaaagaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
     9601 aacaaaaata gctcctccta tttttcgttt ggttgggctt attaccgcag atgcaagctt
     9661 ttgaacaccg tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagata
     9721 caaaacggaa aaatagcttc tcatattttg ccgttttttg agctaatacc gaaggacagc
     9781 tgattgaaca ccgtttattc ggaaaaaggg actgtgacac gacttttgtc acagccccta
     9841 aaaaaaataa taaatttgta ctattctatg aagcttcttc ttcagaaaac tgactgttat
     9901 aaagatctgc atagaatccg tctttttcca tcaatgtatc gtggtttcct gtttctacga
     9961 tcgaaccatg attcatcaca ataatgttgt ctgcatcgcg tattgtagac aagcggtgag
    10021 caacaacgaa acttgtccgg ttttccaata ggcggttcat cgctttttga atcagtattt
    10081 ctgttcgtgt atctacacta gatgttgctt catcaaggat caggacatct ggatttgcta
    10141 agaatgcccg cgcaattgtg attagctgac gctgcccttg cgaaatattg cttgcatcct
    10201 cgtttaatac agtgttatat ccttctggca atttacgaac aaaatcgtct acgtgagcag
    10261 ctttagcagc ctcaatgatt tgttcgtctg ttgcatcgtc gttcccatat ttgatattgt
    10321 catagatact tcctgtaaac agccaagtat cttgcagtac cattgaaaac tgtgcgcgca
    10381 attcatcacg tgaaagatca cgggtatcta caccatcgta acgaatacta cctccagaaa
    10441 catcgtagaa ccgttcaaga aggttgatca gtgtcgtctt ccctgcacca gtcggaccta
    10501 caatagcgac catctcacca ggtttgacat ccaagttaaa atcagtcatt agcaagttat
    10561 ctggtgtgta gccgaattga acattttcaa aacgaacttt gtatggtgta tctttttctt
    10621 ctggaaggcc agatttttca ttggacatct cttcttcatc caacacttca aatacccgtt
    10681 cggctgaagc aactgttgat tggatagtgt tcatcaaact tgcaagttga gtgattggtt
    10741 gagagaactg tgttgtatat tgcaagaacg cttgaacatc ccctaatgtc atgttcccat
    10801 ttgcaacttt tactccgcct agtacagcga cgaacacata gcctagattt ttaacgaaat
    10861 tcatcaacgg catgatgatt gccgaaataa attgagcttt ccaaccagcg gcatacagat
    10921 tctgattttc tttttcaaat acttcctggt ctttttcagt atgattgaat gttttaacaa
    10981 tggtatgacc accatatgtt tcttctactt ggttattcaa taaacctaaa cttttttgct
    11041 gatctgcgaa aaacacttgt gatttcggcg ctacgatcat cacgatgatc aagcttaaag
    11101 ggatcatcaa taacgcgact aacgtcaatt gccagctgat tgaaagcatc atcacgatga
    11161 ccccaatcaa tgtgactgca ctagttacaa attgagtcaa attttgctgc agcgtgcttg
    11221 cgatgttatc catatcattg attgctcgag acatgatatc cccatttgta tgtgtatcat
    11281 aataggaaat cggtacacga ttcattttgg cttccaaatc atgacgtaat tcataaactg
    11341 ttcgttggga aacacgtgtc ataataaatt gctgtaaaaa gttgaataca gcagaaatca
    11401 aatacatcac gattactgtc gcaatgatct gcgcgatttt atcaaagtct atcggaaatt
    11461 ttgttacttg ttggccttgt tgcatctgtt gagcgccagc cattacacct ttgaatattt
    11521 ctgtggttgc tttccccaag acctttggtg tttggatttg gaagatcacg gctgctattg
    11581 ctagtacaaa tacagcgatg atcgcaacca tacgtttaga catatagcca aacaatcggc
    11641 gtaccgttcc ccagaaattt ttaggctttt caccttttac accgagattt ctgccaggtc
    11701 caccatgtcc tggaccgcct gctggtccat gagagggacg tgtgtttgtt ttactcatgc
    11761 taagtcctcc tctcttagtt gtgaatgaac gatttcttga taagtttcgt ttgttgctaa
    11821 tagttcttca tgtgttcctt tgccaaccag ttttccttca tctagcacaa ggatcatatc
    11881 tgcatccatt actgtactta ctcgttgagc aactaaaacg gtgatacttt ctttcatatt
    11941 cgttttcaat gcttggcgaa gattggcatc cgttttgaaa tccaacgctg agaaggaatc
    12001 atcaaacaca tagatatctg ctgttttgac taatgcccgt gcaattgcca gacgctgtct
    12061 ttgtccacca gagaagtttc ctcctccttg ttccacatgg ctatctaagc catcttccat
    12121 ctctgaaaca aaatctttag cttgtgcgat ttctaatgct ttccaaatca tttcatctgt
    12181 tgcatccggc ttaccatact gcatattgtc tcggatcgtt ccagagaaca gaactgcttt
    12241 ttgaggaaca aagcccatta attctcgcaa attatattga gtcatgtcac taatgtccgt
    12301 tccatttatt tggatcgaac cagattcgat gtcatataat cgagggatca aattgactaa
    12361 ggtcgtttta cctgaaccag tcccccctat gattgcgacg atctcgcccg acttggcttg
    12421 gaaatcaata tcttccaatg caagtttttc tgctccatga tatcgataat tgacatgatt
    12481 gaaggccaat gttgcgtttt ttccagcaaa agaaactgtt ttaggatttt cagggtcatg
    12541 gataccgatt ttttcatcca acacttcatt aatacggtca gctgaagctt gtgcacgagg
    12601 caccatgata aaaatagcgg aaagcatcat aaagctgatc aagatctgca tcgcataagt
    12661 cataaatgca atcagattac cgacttccaa agtcatatct gctatgtagt gtccaccaaa
    12721 ccaagtgatt gctacgtttg taccgctcat gatcaatgtc atcattggca tcatcaatgc
    12781 gacgatcgta ttaactttga ttgccgtatt tgtataatct ttattcgcta aatcaaaacg
    12841 attttcttca aagcgtgtct tatcgaatgc tcgaatcact ctgacccctg tcaatccttc
    12901 tcgaaatacc aggtttaaac ggtcagtttt cttttgcata cttttgaata atggaacagc
    12961 aaagtacatg attccgccga tcaaaataat cattactggg atgacaacta agaagatttt
    13021 tgtcaactga tgatcttttt gataagcaag tatgctcgcg ccaatcaacg tgattggtgc
    13081 attgatcatc atacgtaaaa acatttgtgt aaccatttgg atttgagtca catcattggt
    13141 cgttcttgtg atcaaagagg ccgttccata tttatcaaac gcatcttttg tcagactctc
    13201 tgatttttta tagacatccg tacgcaattg cttacccagt ttctgtgatt cacgagtagc
    13261 aaaaaatgta tttgcgatag cagctaagat actgatcaaa gaaaagccga tcatcacaaa
    13321 acctgtatgc caaatataat cgacatctcc ttgcgctacc cctttatcaa taatattgga
    13381 ggtaagtgtc ggcaaatata aatctgccag tacttggatc accatgaaaa ctgcagctgc
    13441 aattgctgaa gtcaaggaga tccgttttac gatttttagc atctagttcc cttctttcta
    13501 tttatatact ccgtttttca gccaagaaag tttcaaattg aattcactga ttgcttgctc
    13561 tactgagtaa tcttcagatg ttgaaaataa gcgatacgaa tgcgcaattg ttgtaaatac
    13621 agtattcatc actaattgga tcagtaattc caattcatga tcatttttta ctttcagctt
    13681 cgtccgatcg atcaattcaa gcaactcttt tgcatcttgt ttgtgttctt tatgtggatc
    13741 gtgaggcgtt ttttggcgac tcgtatgagg agcgactttt cttgaggcac ggtaatccat
    13801 gttcataaac aagttacgat aaaaagaagc attctcgcct tccaaaatct cataaatcat
    13861 tttagaaaaa taggattcga atcctttgaa tagatcacca tcggattctt tcattagttg
    13921 taacatatct ttagagctgt cacgtctcat tgtctcaaag taataaaaat aaagatcctc
    13981 tttatcttca aaatactgat agaaactgcc tctagggata tccgctaatt tgacgatatt
    14041 agcaatcgaa gcttctttta agggtactct agaaaactcg attctcgcag cctcgattag
    14101 tcttttttgt ttttctttag gcaaatggaa aaatgtctgt gtcggcatat ggatcctcct
    14161 ttctttcaag atgacacatt gtcatatgac gctgtgtcat tataacacct cctacctgtt
    14221 ttgcaagaca tttgcatagt ttttttcatt atcatccgat acattaaaaa agtgataaga
    14281 ggaatccttc catttgaatt aatgagagtt gcccattaaa atagccacat actaaacaaa
    14341 ggatgagata aatggcccaa tcaattagtg tccaagactt caataaattg cctctcgatg
    14401 ctgacagcat cgttttagat acaagaaata ccttagatta cacaaaaaac cacttaagcc
    14461 actcgctttc tatccccgct gctatcttgc caagacaatt aaaaaatcta gatccaaaga
    14521 aactttatta tattctaagc tatacaggca ggcgatcaga agcattagcc gatcaactta
    14581 ctgctaatgg ttttcgtgct gtttatatca ttggtgggat ggaagctctt cagtcgagtg
    14641 ttgcttgaat attgcttgtt agattcaaaa gaggctgtga caaaaaatcg tgtcacagcc
    14701 tcttttatac cctgtctttt tttagttatt tattcttcaa ccttatcaat atgacttgat
    14761 agccttttct ttttctcata gttataacaa tcatagcaat aaaaggagaa ggtaagcata
    14821 tggcatctgt tttagtagtc aaaggtcatc ccctaactgc agaagaatcg cgcacagtta
    14881 aggcattgac gtctttttta acaagttata aagaaaatca tccagatgat gaagtgactg
    14941 ttttggaact ttatcgcgat gatattccgg aaatcgatga agaattatta tcaggttggg
    15001 aagcattacg tgctggtgca gaattcactt ccttatcaga aagtcaacaa caaaaaattg
    15061 ctcgtttcaa tgaattgact gatcagttcc ttgctgctga taaaatcgtt atcgcaaacg
    15121 ccttatggaa tctaaatatc ccaacaaaat taaaagcttg gttcgatacc gtcaatgtag
    15181 ctggaaaaac gttccgctat acagaaaacg ggccagaagg attagtgaca ggtaaaaaag
    15241 cactgcacat ccaatctaat ggcggtgtat acaacggaca agactttgcc tctcaatatg
    15301 taaaaggtat tttaaacttt gtaggcatcg atcaagtaga tcagctattc atcgaaggaa
    15361 tcgattatga ccctgaccgt gcagatgagt tgatgcaaaa tgcattagat aaagctgctg
    15421 ctttaggtaa atcattttaa aaattgagat aaatgcatac aaaagagaca gaggaaaaaa
    15481 tcttcaaagc agcatttgta ccttggatat tttcccatct gtctcttttt gttattcttt
    15541 ctgctttttc aacggatttt cttcataaag agaagagatt gttgtgtacc attcaaaaac
    15601 atgagtaatc actactttag ctgctgcata aacaggaatc cccaagatca caccgatgat
    15661 tccgaacaat ttcccagaag tcagcagaac taataaaatc gttactggat ggattgctaa
    15721 ctgattccct agtactaatg gagagatcaa gcgtccttct attgtctgct ctactgcaaa
    15781 aacgatcagt actttgatga tcatgaatgg acctgctaca atggctaaaa agatagccgg
    15841 aatcatcgct aaaaatgatc ctaagtaagg gatcaggttc aaaaaacccg caataatccc
    15901 caacgtgaca gcatactcta aaccaatgac cgcaaaacca atcataaaca tgattgccac
    15961 agcaaaagcc acagttagtt gtcctcttat ataagaagaa acttgactat tcatttcact
    16021 cagcacattc aacgtcggtt tttgcatgcg tgtcggtaaa aatttgacaa aataaggtgc
    16081 taattgttta ccgtctttca atagataaaa taagataaac ggcatcgtca gcagagccac
    16141 taaaatagtc gctaccgcgc cgacaaaact tccaagactc tgaactgtac tcttagatat
    16201 atcttggatc atatcgcctg ccgaattcat gaacttatca cctgctgttt ccaactgttc
    16261 gcgaaattgg ttgaacagcg ggtcccgcaa aatctcggtt aatttattat caatcgtatc
    16321 tacatattga gggaagttac tgatgaagct cactgtttgt tcctgaatct tagggatgat
    16381 cactacgctc ccccatgcga ttagtgcaac aaccaaaaca aacagcccaa tgatactgta
    16441 aagccttggt acttttcgct tttcaaagta atcaactacc ggattcatca agtaatacaa
    16501 gattcctgct aaaatgatag gcagacctac gattgctaaa aattgccaga ttggtgcaaa
    16561 caaatatgaa acttttgtaa ataaaaagac gatcagcaat attaacaata caacaagtaa
    16621 ggctgtgact acttgattat ttaaaaacca gcgccaaaac cacgataccg gtctgttctt
    16681 cttatttttt ccttccataa ttcctcctgt tagttataaa aaatttggct tcccaaacca
    16741 atagcaggca gtctatgcgg atgaagatag attccatttg caatttgtcc ttcttctgga
    16801 caagcatcaa aaatcaatga tacgtgagct actgtctcta aattcccatt ggccatcgga
    16861 ccgacataat cgatcgtata tacctgatca tcaaaagcaa ttgttccgcc tgctttcaaa
    16921 gaaaaaggtt cgtcttttga tacttcttga atcaccgaat attttcgtaa ggcagctgtt
    16981 gctgttttat cgaacaaaat cagcatgggt tcttccttgt ttattgcttc tgtcccaatt
    17041 tctgtaatct ttgcctgaat cattgcactc tctcctttaa ttgctttcaa aaacagtata
    17101 acacattctc attctttagc cgtgtggaat aattatgtga tagaataaaa acgaaaacta
    17161 tttcattaca attaggagga atcagctcat atgatcgaaa agataatttt aggaacctat
    17221 acgcgtcgcg aaagtaaagg aatctatacg atcgaccttg atacagaaaa agaagaatta
    17281 gccaaccttt cattagctgt tgaagaaaat agccctacct atgtagcaaa aagtaaagct
    17341 ggcaatcttt acacagttac tagtgtggat ggattaggtg gtgccggtgc gtacgataag
    17401 gattttcatt ttttgaatgc ggtaacagag tctggcgctc ctttgtgtta tgtagcggtt
    17461 gatgaagcac gtcaattact atacggggcg aactatcata aaggtgaagt aaatgtctac
    17521 catatattag gagatggcga actgaaagca gcagattcaa tattccatca agaagcgaca
    17581 ggtcctcata aaaaccaaga tcatgcgcat gtccactata cggatctgac tcctgaccaa
    17641 cgattagtcg tatgtgatct tggtacagat cgtgtgtata cttatgatgt gtccgaaaac
    17701 ggcaaattga atcttgtaac taccttcact gcagagccag gaacaggccc tcgccatctt
    17761 gtctttcatc caaatggaca attcgcttat ttatttggtg aattagacag cacagtccgc
    17821 gtgctttctt ataataaaga aacaggtgct tttgaagaat tacagaaaat atcgaccttg
    17881 ccagaagaat tcgacggtga aaatggaggg gcagctatcc gcatttcaaa tgatggcaag
    17941 ttcctttatg cttctaaccg aggacataac tcgattgctg tattcgcagt ttttgaagat
    18001 ggttcccaca ttcaaaacaa gcaaatcatc tcaacagaag gggattttcc ccgcgatttc
    18061 gcactagatc caagcaataa ttttgtcgtg tgtgccaatc agaacaccga taatctgaca
    18121 ttatatcgcc gtcatcctga aacaggatta ctggaattac tgaaaaaaga tgtctatgta
    18181 ccagaatgtg tctgtgttta ttttgatcgt tagtaagaaa aggagaatga aatgaatcag
    18241 caagacgtat tagacagact acgggaagaa ctaaatattc ctttctttga tgggaaaatc
    18301 gaagataaag agtatagcga agaggaatat cagaaactca aagccgattt acagaactac
    18361 tttgaacaat atgtacagaa tgtagaaaac taatcagctt tccatactat ctgaaaagaa
    18421 catcaggaca agacttttcc gtctatcctg atgttctttt ctacaaataa aaaaccgccg
    18481 agatggttag actcgacgga aaaggagtat ttactcgatt ttgagtaagt acaaggttgt
    18541 tgttggttat aggtatgtca ttatcataaa ggattttgat cactctatca taaaggtaat
    18601 attacattta tattagataa ctctagtttt tattgttatc aaagatttct tttatttttt
    18661 caacaacaaa ttagtcattt tttggatggc agctgtcaat tggtctgtcg cttggtcttg
    18721 ttttgcttca gatgtattga atagatcttc atctacccct gattcaagat cgatactttg
    18781 gatcatttct tcacagcctg ctacatatga aagataagct ttttcaaaat ttttatgaat
    18841 gcccattgct tttgctggtg gacgcaaagt actgactttc ttaagcattg cccgatattt
    18901 ttttgttcct tcttcaaata acaggatgat ctcttcaaga gtcgatttgt ccagttcact
    18961 tgtttttcct tcatcaattg cgtttctgat cacttcatat ttcggatgca ttccttctcc
    19021 gattgcttct gtttccttga cgatttgatc gatggactgc acataatagc caatattcgg
    19081 taacatgttc gttttcctac tttctccatt gttgttatta tttattctac ttgtttttat
    19141 cctcaaatga caagtacttg aaaaaattta atggtttcta tacagctcgc cattcttatt
    19201 cgtgctcaaa gaaaccatct gctaaactat attgtttccc tgcgacaatg tcatattgga
    19261 tcaattgata ttcgtcgtaa atctcttgtt gcgagcgatt gaattgcgct tctttatacc
    19321 actgtccgtt ttgatagtac agttctcgtt caaacgccgg aagttcctgt tctaatgata
    19381 aaagcagctg ataaaagcca tttgttgtct gattcgtctg ttccattaga ttcgctgcaa
    19441 aataaaacgg actggtgatt gcatcgtgag tcgtttgctt ttctaattct cctttactgt
    19501 cgaacataag aaattgtgtt tcatgcaatg tttgtttgtc attttttgct tggatcgtat
    19561 ctgaatagat ccctggaaga tgatcccccc aaaaaacaac taaagtcctt ctagaaagat
    19621 tcttcaattc ttccgtgaac tgcttcagtg ccgtgctgct ataggaaata tcttgcagat
    19681 aattctctaa gtcaagtgtc ccacttccat ctgaagtttt tgcaggatag tctagattat
    19741 catactttcc atcgtagggc atatgtgtct gcatcgtgac taaatgaatg aactgaggtg
    19801 ttttttcttc cttgagaagt gtcattactt ctttgtaagc tgactcatca gaaatatatg
    19861 gattattctc tatcgtatcc gtataggtca tcgtacgctc gctgatgaac tggtcaaatc
    19921 ctaatgtctg ataaacatcc tctcttttat acatcgaagt attgtatgga tggatcgctg
    19981 tcgtctggta tgattgtgcg tttaacgctg aaacgataga aggaagctga tccatttttg
    20041 gtacaagcat cgtgtaaggg gtggtgagtt gcgcattaaa taacgccata gaaaaaccag
    20101 tcaatgcttc aaattcgata tttgctgttc caccgccata gttttgagaa agcatattgc
    20161 cactatatgt ttgatcagct acttcataat aatctgccaa tggctcacca gatacttcta
    20221 cgccgtttaa tcgactagga tctgagaaac tttcactcat cacgaacacg atgttcggcg
    20281 actcttcctc tacagtttta tttttttcat cggctagctt ctggtatttt tcagtgattt
    20341 ctttgatctt ttctttcgaa tatccttcag gttcatccat tggttctacc ttcagattgt
    20401 acaaaaaccc accgataaaa ccagtgttat agtagttcat tttctggctg taaggtatcc
    20461 atagtgccgt tttattatat gcttttcgca acaaattatc gggattgttg aaattactga
    20521 tataccctag cagtccaatt gccacaagca gtgtcgttaa tcggatcaat tggaatcgcc
    20581 ttcctttttt caggcttcga aacatgtacc agcaaaacaa tcctatcacg gttcctgcgg
    20641 cagctaagat cactgtaaac agcatagtgc cggtcatatc tttcagcaat ccgatttcag
    20701 tgatcatctt caaatcgtct ggataaatcg gttcttgccg ataactcatt ttcatataat
    20761 ttgcaaaacc taatacaccg atggctacga catacgtaag ggaacctaaa gggacagatc
    20821 ctgctagtgc aatcagaaaa ataagaataa tcagcaagac gagacaggct aagaagaatt
    20881 tttccgtatg ccaagaaaaa gcaaatttca acgcaaggtc tacggataga tcattttgac
    20941 accactgcaa aaataaatta ctaaaaacaa caagaaaaaa gatactgatg attgctgctg
    21001 ttccttttaa aatctttttc tgtttggtcg aaagtctgct tatatatctt ttcaaatgct
    21061 tgctccttca tttctatcaa taaaagaatg ttgcttctct tattttagtt gtacgaaatt
    21121 cgaaagtaaa gaacggaaag aagaattaat gaaatccaaa tattctcttt ctttatggcc
    21181 tttctggttt ctttaataag cggacctttc gtactccaga aattttttct agctgttgtt
    21241 tcacttgatt gagctgttca aaatcagtac tgtctatgtc aatcaatatg tagacatgct
    21301 cattcttatg gttacgcgat atatctgcga tcgctagctc gttctcattc agcagagaaa
    21361 atatctctgc taatatattg gttttctttt gataaaatat agtgaatcga aacggcgctt
    21421 ggaaaaacat tcgtgcggat ggataattga ccgcttctct aaccgtaccg aacaaaagat
    21481 aatcttttaa ggcttcaact gccaaacgtc cgctgtctgc caaagcttcc tgagtcgatc
    21541 cccctagatg aggcaacata agtatacggt cattttctaa gaactcttct tctggaaaat
    21601 cagtcaagta tttagccaga tgttgttttt ccaatgcttg cataacagca tatttatcaa
    21661 caatttcact tcttcctaca ttgataagta cagcatcttt tttcattttt tctatattcg
    21721 cctgatcgat caatcctttt gtatcttctg tcaacggcag catgactacg atgtaatccg
    21781 aggtagaaag aactgtttct aaatctgctt gccgaacata atccaactgg gcatcgcgga
    21841 tagaataacc caagacatcc atccctaagc tatagcaact tagcgctact tttgtgccta
    21901 tcgctccaag accaagaaga ccgatggttt tgccttgcaa ttctcgtccc acataagcac
    21961 ttcttttgtt ttctgcctgt tctaaaatat taggacctgt tagtgtttgt accatacgac
    22021 ttgcttcgat tatcggacgg gaagataaga gcaaacagca caaaacaagc tcttttactg
    22081 cattagcatt tacacctggt gtattcatga cgatcgtccc gttttcagaa gctttttcaa
    22141 cgttgatcgt gttcacccca acaccagctc ttgaaattgc tagcaaatct ggtgtaagcc
    22201 attcatcttt cactttcgta ctacgaataa ctattccttc ggctggcaaa tcatctatta
    22261 ttttgaaata ttccccttgt aacttttcta tctcttgttc tggaatagca tcaatcaaat
    22321 gaacgtgata cataaaaccc cctccttact aacaagtatc tcgaaatttc tgcaattaga
    22381 caactgttta ccatttgact tcctagaagc aaaaagagca gttagagcag agtaggctga
    22441 gtgataaaaa taaggctatg acaagttact gccataagcc ttattttcga gagcaaaata
    22501 ttgagggtga gatatcttga taaatggtgt tccgttagct gacattgata ataagactaa
    22561 atttcaaaaa agttgaaaag caaatttcgt caatctcttc ttatttctta aaactaagtg
    22621 gctttttcac aacctctttt tatctaataa ccactgtttc caactcaact aattttgccc
    22681 acgtttcaat ttgatctgtt gttagattta gcgagacaac ggtatgatga ccgccacctg
    22741 cctgaatcca tgaatggaca ccttcatgga aattcggttt agttttccat aacacacgtg
    22801 caacaggtaa attaggtgct tcagtcgttg gttcaaaggc ttctacttca ttgatcagca
    22861 gtttataatg cgtaccgaaa tctgccatcg atacgactac cccttctcct gctttcccat
    22921 caaatacaag acgcgccgga tcttctctat ctcccattga taaaggagaa acaacgattc
    22981 ttggtttcgt cgcagcaagc gtcggatcta cttccatcat atgggattcc aagattgctt
    23041 cttggccact agctagctcg taagtataat cttccatgaa tcctgtatct ttccctcgag
    23101 ccatgatttt cagcaaccga tcaatcgctg cagttttcca gtcgccttcc ccagcaaaac
    23161 cgtatccttc tgccatcaaa cgttgtacag ctagtccagg tagttgtttc attccataca
    23221 ggtcttcaaa attagtcgta aaagcgttat agcctccttc ttctaagaaa cggcgaatac
    23281 cgatttcctg ctgagcttgt acttttacat gttcctccca tgtcttttct tcataatcac
    23341 catagtcgaa ggtatacaat tccttgtact cttcaaataa ttgatcgact tcttcgtctg
    23401 atacgcgatc gattacttgt acaagatctc cgataccaaa ataatcaaca gtccaaccaa
    23461 attgaatctg tgcttccact ttgtctcctt ctgtgacacc cacgtttctc atgttgtcac
    23521 caaaacgagc cacttttatt ccaaaacttt cattataggc tactgcaaca tccatccaat
    23581 cagcaatctc tttttggatc tcaggtcgtt tccagtagcc aacgacgatt ttattttgtt
    23641 tatttaatcg agcattgatg aacccatatt cgcggtcacc atgagcggat tgatttaaat
    23701 tcatgaaatc catatcgatc gttttccaag gaatgctttc gttatattgc gtagctaaat
    23761 gaagcaatgg tttttgtaga agctttgtcc cgcggatcca catttttgct ggtgagaaag
    23821 tatgcatcca agtgatcaca cctgctactt cttctcgata attgacctct ttcatgattt
    23881 ttgtgatcgt atcagcagta actgctaatt cttgaaggcg gattggataa ggcaattggc
    23941 cattttcatt cagttcatcg accatttctt gtgcatgttt ctttacttct cgcagtgctt
    24001 cttccccata taaatgctgt gatcctacaa caaaccaaaa ttctttttct ccaatattca
    24061 acataattag ccactccttt tatttgtttt gtccgtaata cgcatttttt ccgtgttttc
    24121 gtaaataatg tttatctaaa atccgttgag gcagctcttc tgaaaatgaa ttgagctgtc
    24181 tggtaaataa attcatcttg catacttcat ctagtacgac tgcgttcatt accgcacttt
    24241 gcgcatcttt cccccaagta aatggaccgt gtccgtgtaa aagtacccct ggaattgcta
    24301 agggatcaat tttgcgttct ttgaatgttt ctatgataac ttttcctgtt tcatattcgt
    24361 acccgctgtc gatttcctgt tgtgtcaaaa atcgagcaca aggtaccgat ccataaaaag
    24421 tatctgcatg cgtcgtcccc atcgcaggaa catccaatcc tgcttgtgcc cagatcgtcg
    24481 cccatgtcga gtgagtatgg acgattcctc cgatttgagg aaatgaacga tataaaacag
    24541 catgagtagg cgtatccgaa gagggattga gtttaccttc aatgatatga ttgtccaagt
    24601 ccacaacgac catgtcgtct gctgtcatct cttcataact gactccgcta ggtttgataa
    24661 cgaaatatcg tgtttcagga tcaaaagcgc tcacatttcc ccatgtatat ttgacgagtc
    24721 cgtgtttagg caagtcaaga tttgcttggt atacttcttc tttcagttgt tccagcattt
    24781 tctatcactc ctttttcttg caattgatga atcgctgttt tttcaatgtc gatcccctca
    24841 cggtaacgtc tcataaatat ctgatagcct gctatttctt ctgctgaagg tgtgatggtg
    24901 attccttggt cttgtccaaa cacgtcttct gccaagaagt tttctaaaga cttcccatgt
    24961 cgatccatca tataggctgc taataacgcg attccccacg ctccaccttc tccggctgtg
    25021 tccatcactg taactggtgc ctccatagca gatgctagaa tccgctgtcc aacttctggt
    25081 gttttaaaaa tccccccatg ccccactaaa cgatcaattt ggacattctc agatttcaat
    25141 atatccattc cgattctcat tgcaccaaaa gcactggcta aatggatccg catgaaattc
    25201 gctaaatcaa aacggctacc tggtgtacgt acaaataatg gccttccttc atttactcct
    25261 gtaatgtttt caccggaaaa ataaccataa gaaagtaatc ccccgcaatc cgtatcgcct
    25321 tctagcgctt tgttgaataa taccgtgaat aatttatctc tgcttatctc catacccaaa
    25381 gaattggtaa attcttcaaa aatactgacc caagcattga tatccgaaga gcagttattc
    25441 gtatgaacca tgcctactag ttctcctgaa ggtgtagtga ccatgtcgat ttccggatgg
    25501 acattttcta gttctttttc taatacgatc atagcgaagg cggaagtacc agccgaaatg
    25561 ttccctgtcc gtttggctac actgttcgta gcgaccatac cggttcctgc atccccttct
    25621 ggcggacaaa ccgggattcc tggtctcaaa tttcccgtag ggtcgatcag ataagcccct
    25681 gattcgctta acttgccagc ttcttctcct gctactagaa ctttaggcaa aatgtgatcc
    25741 aagtcaatcg ctacgtgcgc tttttcgatt aactgaccaa aaatattttt catttcttga
    25801 tggtaggttt tcgttgaagg atcgatagga aacatgcctg atgcatctcc tacgcctagt
    25861 actttctcac cagtaagctg ccaatggaca taacctgcaa gcgttgtcaa aaaattaatt
    25921 tctttcacat gttcttcttt attcaaaata gattgataca aatgagcgat actccatctc
    25981 tgcggaatat tgtattgaaa tttttccgtt aatattcgtt ctgcttcttc agtgattgta
    26041 tttcgccaag tacgaaatgg tactagtaac tgttccttgc tatcaaatgc tagataaccg
    26101 tgcatcattg cactgaaccc aatcgacccg attgtcgtaa gattctcgcc atagatttcg
    26161 aatacttctg ctgccatttt tcggtaactg acgcgcagtc ctttccaaat atcttctaat
    26221 gaataggtcc agataccatt ttctagtttg ttttcccact cataactacc agcggcaata
    26281 ggagtaaaag atgaatcaat caatacagct ttgatcctag ttgatccaaa ctcaatccct
    26341 aaagatgtac gaccatctat gacatctaac atactttgcg catttacttc cttcataatg
    26401 atttaactcc ctccttctcg tcaaacctga tcaaaccgtc tttttagtag cgcttacaat
    26461 gattattgta tacttttgta cgtacatgtc aagcggttat ttacaagaat acaataaaaa
    26521 acacttgttt tgtacgtaca taagttataa aatgaaattg cttacaaaat ctgatcaacc
    26581 gccttctcga aattctagaa ggagagaata cttatgaaaa aagaaaaaaa agtttcctca
    26641 tcgtttattt atttttttgg cgcatttggc ggcattctat ttggatatga tattggtgtt
    26701 atgaccggcg ccttaccttt tttacagcat gattggggtt tggcaggtaa agcttctttg
    26761 ataggatgga tcacgtcttc ggtcatgtta ggtgcgatcc ttggcggctc tttatctggt
    26821 cttctttctg ataagcttgg acgacgaaaa atgattcttc tttctgcact tatttttatg
    26881 gctggttctg tcctttctgc atctgcccct cacaatggtt cttattttct gattgcagca
    26941 aggattcttc taggtttagc tgttggcgct gcatctgccc tagttcctgc ttatatgtct
    27001 gaaatggctc ctgctagact aaggggaaga ttgtcaggga tcaatcaagt gatgattgct
    27061 tctggcatgc tcctttccta tgttgcagat tatttgctaa aagtgttgcc tgaaacgatg
    27121 gcttggcgcg tgatgttagg gctcgctgct gtccccgcgc tgattttatt tttcggtgtt
    27181 ttagctctac cagagtcacc gcgttttctt atgcaatctg gccgcttgga agaggcaaaa
    27241 agagtattga actatattcg gacaccaaaa gaagctgaac aggaattcga acaaatccag
    27301 ctgaatgtca aacaagaaaa gacaaccgga acttcttggc atactttgtt tttagaaaaa
    27361 tatcgttcct tagtcattgc cggtattggt gtagcagtat tccagcaatt tcaaggagct
    27421 aatgctattt tttactatat tccgctgatc gtagaaaaag cgactgggca tgcagcgagc
    27481 gatgcattga tgtggccgat catccaagga atcattttag ttgctggttc tttgctcttt
    27541 ttggttattg cagacaagtt caatcggcga actttattga aaataggcgg tagcgtgatg
    27601 ggactttcat tcatcctacc agctgtttta ggaactgtac tggatgctca tacaaattcg
    27661 ttgttgattc tcttgttttt atgtatttat gtcgcatttt attcttgcac atgggctcca
    27721 ctgacatggg taatcatagg cgaaatattc ccactagctg tccgcgggcg agcatctggc
    27781 ctagcctctt cttttaattg gattggttct ttcctagttg gcttgctttt tcctgtgatg
    27841 actgcttcta tgtctcaaga aatcgttttc ggcatttttg ggatcatctg ctttttaggg
    27901 gttttattta ttcaagagat cgtaccagaa actcgtggga aatctttaga ggaaatcgaa
    27961 caaagtgcat ctaaaaaaac ttatcctaaa cgtatttcta tccatcattc ttaaagaggt
    28021 tgtgacagaa gtggtcagct tcaagaaata agaaggaacc gcttagtttc cagtcacaag
    28081 gaacaatttc aaaaaacagc ttctcatgtt tgatgaatat tgtgacttgt gaccagttgg
    28141 taggagaaca ccgtttagtg gagaggtggt gaaagaaacg ttcagccttg agcgatgaca
    28201 aaagctctca caaacattgc gacgagtaac cgtaggagca ataagaggcg gtaaaaaccc
    28261 gtctcttatt tttcgttttc tttcactgtt tcccgttcaa ctagtacagg ttcatagaga
    28321 atatttggct gtgcctgtcc tttttcaatc gttgtgacga tccaccgtgc ggcatcttgt
    28381 cccaactttt cttttggatg cgtcaatgtt gtcaattttt tattccctaa atggcttaaa
    28441 gaagaatcgt ctgttcctac gatcgatagg tcatctggaa tatgatagcc attttctgtc
    28501 aacttatcta gcattaaact agctatttga tcgttatagc aaatgatgcc atctaaagct
    28561 tgttcttctt tcaaccgttg tactgttttt tcagcgattg tttctcgtgt ttccgtggtg
    28621 aaagtaatga tatcttccgt caaaaaacgc acattatact gttcacacgc gctgataaaa
    28681 cctttcatcc tgtattttcc ttgaagatcg tcgatttttg tgatgaacaa cagacgctga
    28741 tgtccttttt ttatcaaata ttctgctgcc ataaaaccga ctttcacatc atctaaacaa
    28801 atataagagg catttaactc ttcataagca gcattgatca tcaccatcgg aatgccttgt
    28861 tctctcaaag caacataaag tgctaagttt gggttatact gattactttt cgtaggctca
    28921 acgatcaaac catctacccc ataagcaatc attttcttca aacactcttt ctccaattcg
    28981 tgatcgttat tcgtactggc taaaagcaat gaatacccag attctcgtaa ttcattttcg
    29041 attcctcgga taatcgacgg aaaaatataa tcagagagat atgtggtgat gacaccgatc
    29101 gtttttgttc tgttcaatgg tgttttttct ttcttatagc tgtcatctac atatgtgccc
    29161 gacccttttt ctttacgcag atagccctca tttaccaaca cagcgattgc ttgccgaatc
    29221 gtatgtctgc tgacgtcata tatttcttgc agcttgaatt ctgagggaat ggttgtattt
    29281 acttcgtatt cctcacgaag gatcttttgt tttatctctt ctgcaatatc ttgatatttt
    29341 gccatttgcc ttgccccttt ttatttgtac gtacatatat cataacgagt ctgcaaggtt
    29401 tattcaagca aaaatcaagg tttcttgtgc tcataaggca tacggaacag gttcaccaaa
    29461 ttctggaaat ccttgctcat tccacgtaaa gcattgcgcg ttcgcgtgcc gattaggatt
    29521 gtctaacgga tccccttttt ggttcttctc tggtcgagca tgatagatca aaatatcaaa
    29581 gcacccgtct tctgaaaccg taaacgagtt gtgaccagga ccaaattgct tatttttttc
    29641 agaagaaacg aatactggct ccgataattt atgccatgat tcttttaata aaagattctt
    29701 gtgctcatca gcccacagca ggcccatggc atagttttca tctgttgcac taccagaata
    29761 cgtaatgaat attttcccat tgcgagtaag gactgctgga ccttcattta ctgaaaaacc
    29821 ccgtttttcc cattcatact caggtataga aaggaggatc tgcttcccct ttaacgtcca
    29881 aggattatcc atttcggata gataaagatt agaattccca gtaatttcag gatcttgttg
    29941 ggcccacaca taatatagct ttccttgaga agagaaagtt gttccgtcta aactgaaagt
    30001 ttcccttttc gtttttattt gtcccttttc tatccaatct gtattcattg gatctatatg
    30061 ctgattttca agtacgaaca tgcggtgatg atgagattca tcacgtatag ttttatgatt
    30121 gcttgcagca aagtaaatat accattttcc attgatataa tggacttcag gtgcccagat
    30181 caattcactc atcggtcctt cttcatgagc ctgccataca atcaggcttg cagcatgttc
    30241 taattcattc agtgactttg cccttcttaa ttcaatcact tggtagcctg gaaccgatcc
    30301 tgtaaaataa taataaccat ctgtatgttt atatacccaa ggatctgccc gttgccgaac
    30361 gataggatta ttataaagtg tcttcatcat aacaaatttc ctcacatttt ctttattcta
    30421 attacattcc aagagtattt ttctatccct acatttaatt cgtcatgttt ttctattgaa
    30481 aaatagttta tttcaggttt tattttttct ggttcttcac gtgtattttt gtccaacaga
    30541 tcgctactgg ttaatgtgta gtgctctcct tgatctagca aattgaaatc atgttcaatc
    30601 gttatctttt gttgttcatt tgtgcgattg atcaagaaaa tagtcagctg attgccgtta
    30661 tctacgatca cactgtccac ataaggaaca ttttttatct tttcacaatc ataaacaggg
    30721 caaatgattt gttgtttcaa tacttgacct ttagcatatt tcgataagtg ttgcatcaca
    30781 taataagttg gattcctcca tgcttgtcct ttagcatctg tcagtattaa aggtattgtg
    30841 tttactaaca atgattgaca actaatcttt acgcgatctg cgtgtcgtaa aatagccagc
    30901 cctaatgaac caaataataa tgtatcttcc atagtaaagt aacaccaatc gatgggacta
    30961 ccgacttcaa aatccctatg tcttttttcc ggttgggcat gtacattcca ttcatcaaac
    31021 gctagataca ttgttttctc acttctcaaa tatcctttta cataatcaca agtagcaacg
    31081 atttcttcaa tttgacgatc gaatcgataa gcttttgcaa ggtatgtcgc tgtatcatca
    31141 cgctcatttt taggaggttt agtcaagtct tcttcatcat acttatcaat atagttatgc
    31201 aaagctaaat aatctacatt ctcataagct tcttctaatg cgatacgatc ccattcaggg
    31261 tacgatggta aacgtggcgt agagcttcca actaagattg tttcaattga atcatccact
    31321 aacttcatga cttttgaggc ttcatttgct aacctaccat attcataagc actcttagcc
    31381 cccacttgcc atggaccgtc tagctcattc cctaaacacc ataatttaac gccaaaaggc
    31441 tttttttgcc cattttttat tcttaaatca ctccagaagg ttccttttgg aaaattacaa
    31501 tattctacta aattttttgc ttcatcaatt ccttttgttc ctaaattcac cgtgaatatc
    31561 ggtttcgctt gtacatgact gatccattta aaaaattcgt ggagtccaaa agaatttggt
    31621 tctatacctc gccatgctaa atcaattttt tttggtcgtt gttctttcct acctacagta
    31681 tccacccaat cataaccaga tgtaaagttt cctccaggat agcgaatgac acctaattct
    31741 aattccttta cataagagag gacatcttgt ctaaacccgt caccatcagc tgtttcatgg
    31801 cccggttcat aaatccctgt atagacgaca cttcccatat gctcaacaaa tgagccaaac
    31861 agccgagaac tgatctctcc taccgtaaaa tcctctctga tttttacttt cacttcttcg
    31921 tccaacagct ttccctacct ttcatttcct atagtcaaga taatagatac aagtttccat
    31981 acgccaccag caaagataaa aataaaagga gggaaaagaa atatgagcag tacaaatcct
    32041 aataacaata cgcaacaaag aatatttctc aaaaaatcaa aaaatacttt ccatgcactt
    32101 tgttcaaaaa tttgtaaaaa tgtgatatct ttgttttcag aaatcacata tatccaggat
    32161 aaaagaaaca tggaataaat aagtaaaact acatatccac taattgaaag tacgattgtc
    32221 tgttcttcta ataattgatt aacaagaaaa taaataatga tcgttaacag actaaggaac
    32281 aagttcttac caaaatgttg ctttattcct tgaaagaaaa aataaatgat atttcctgat
    32341 ccctcacctt tccattgcgc cattacataa aaaacagcac atgtagcagg cacgatagta
    32401 ataacaggca aacacacgat tacccagaga atacctaata agatgtaatc agaaaccaca
    32461 atcaactgtc cataaaattt agtctcaact attttcatct tcctcactct ttcattccag
    32521 acatagctgt tccttgaaca aaatatttct gtgcaaaaat ataaaccact agtaatggca
    32581 gcattgaaat aaccgttcca gccattacag gtccataaaa agaaatgttc tgccctgtaa
    32641 ataaagctaa ccctgctggt aaagttctca tttttgaact acttgtcaat atcaatggat
    32701 aaagcaagtc attccaacta ttcatcagcg tgaaaatccc taatgataaa agagtaggtt
    32761 tgctcaatgg caacatgatt ttcaaaaaaa tattaaactc acttaatcca tcaatgcgtg
    32821 ctgcctcttc caaagaatct ggtaaagtaa taaaaaagga acgcatcatg aagataccaa
    32881 atgcagttgt catacgagga atgatcaaac ctgcataagt atcaagcatg ccaagcaaat
    32941 tgacttcaat aaatagagga atcatgaaga cttggaatgg gatcatcatt gtcagcaaaa
    33001 cgaaatagaa caaaactgat tttcctttga aacgcatacg agcaaatgca taccccgcaa
    33061 gagaatcaaa gaatacagaa gtgatcactg tccctccagc aaaaatcact gtatttttga
    33121 tgtaatctaa taaagggatc gttgtccaaa ccttaatata gttatctagg gtatagctat
    33181 ctgcaaatat tctaggcgga aatgagataa tgtctttttc gtgtttaaaa gaagaaataa
    33241 tcagccacaa aaaaggaaac gcaataacca acaatataac tagcatacta atcgttcgaa
    33301 tgattttttt accactaact gcttttcgac tattcacagt aactgtaatc gagctttctg
    33361 gtgccataca ggtttccctc ctatttcatt tgtttttctt tccgtgacat gtacacatta
    33421 gtggagatag ctatagttgc aacaatgata aacaaaaccg aagacagtgc tgatgcatag
    33481 cccaattcat aaggtgctgt aaatcctcga ctgtaaatat attgaacagc tgtttctgtc
    33541 ttaaattgtg ggccaccagc ggttgcaaca tatacttggt caaacacttg catcgaacca
    33601 atcagattcg ttaaaataca aaaacctaag gagggaacga tctgaggtag cgtaatgtta
    33661 aaaaactgct gtcgatttgt tgcaccatcc atttgagcag attcatacaa agagggagag
    33721 attccctgaa tagcagtcaa gagaatcgtc atagtaacac caaaattctt ccaaacagtc
    33781 atgatggcta ccgtcggcat agcaagggtt ggatcgcgaa aaaattgagg gatcggcaag
    33841 ccgacttgtg aaaacaagta aggaatcgcc cctacattcg gatcaagcaa catagaaaaa
    33901 ataatcgcaa ttgacgtcaa tgaacaaata actggaatgt aaaatgtcga acgacaaaaa
    33961 cgattgaatc tggtattttt agataatgct actgctgcta ataaaccaac gataatttgt
    34021 gttggcgttt cgagtacggt gaaatataaa gaatgaatca acgaattaac tgctcgttca
    34081 tcactaaata tacgtgtgaa attttcaaaa cttacaaaat ttcttgatgt aaagaaaata
    34141 ttcaaatctg taaaactaat agcaaatgtt cctaaaagcg gtatgacagt aaataataat
    34201 aaaactaaaa aagatggagt taaaaacaaa tacgcagcac gatcaggacg attccaatat
    34261 ttggtgaatc cgttcatata gtccacctca tctttcattt tgataaaata tacaatcttg
    34321 gtttaaacga atctgaataa gatatccgaa caatcgctgt gatttttgca ataacaccta
    34381 tctgcaaata ctaggtcgat cattcagata tctgtatctt gccaacagtc tatcctgtat
    34441 aaagttctgt actatttttg cagtaattga ttgatggcat catctgcatc gttcatgagt
    34501 gtctcaggat cttctcctgc taataatttt tcaatcagtg gattgatgat atcgttattg
    34561 atcgcagaga tttgggtaaa acctggtaaa tatggctctg catagtccat ttgtttcgat
    34621 aattcagata caataggatc attttttact tcttcatctt ttgctactga atccaagtag
    34681 gcaggaaaac cattttcgat actccatttt ttaccaattt cagttgtatt ccaatatttc
    34741 aaaaactcat agacagcttc ttttttatta ttatctgtac ttgccggaat ggcatatcct
    34801 acgccatcta aaatagctgt ttttttgtct gaatcactcc cttgtggtaa ggtagtcaca
    34861 ttataatcaa tctcgtttgc tttcaatccg ttatttaacc atggcccatt gatttcaatg
    34921 gctagttgac cagcattcat taaattgtcc atttcggcgc cagaaatcga ttctggacca
    34981 gtcttgtcta catggatcat tttttggatc gcttttaacg ttttaaggtt ctcaggagac
    35041 gcaaattcag acttagtata atcatcgttc actaatttac cgccatttgc taacatccag
    35101 ttatataacg gtgcagttcc atccttattg aagacaaaac ctgatacatt tttactacta
    35161 tcagttaatt ttggtgccat ttctgtcaat tcttcccaag tagtaggtgg ttgttcagta
    35221 tctaatccag cttgtttaaa caaatcatta ttccaataca tatacatgcc ttgtacctgc
    35281 atggggacaa aatactgttt accttctact tcacctaatt caatagccgt ttctttaaaa
    35341 tcagatttat ctactccatc atattcccag aaatcgtcta atggttgtac cgctccgttt
    35401 tctacatact gtgcaaaatc tggataattt aatgcaataa agtcaggtgc ttttttagaa
    35461 gaaatcgctg taggaagttt ttcattcaaa ttggcccaag tcatgatatc catttcaatt
    35521 ttgatgtttt tatcatttgt tttattaaat tcattaacga tttcttgcaa tatttcacca
    35581 tcagatgcag taaacccatt ccaaaaagtc aaagtcactt ctttatcaga atcagattct
    35641 gctgaacttc cgccaccgca agctcctaaa atacctgttg cagctaccaa caaagagcca
    35701 attaaccatt ttttaccttt cattttttgt tcctccttca tctgataaac gacatctgac
    35761 cacattccaa gatagcggtt tcaataaaca attgattttc ccatcttcag cttttacatt
    35821 tgttgaatga cttggtttta cttgttcact tttaggaaga ttagttgctt ttgtatcaaa
    35881 tccactcatt tcagaaaaat caatcactcc ctctaatgat aattcagaca agtgaacagt
    35941 tagattcatt tcttcttctc cacggttaac tgcaaatatg acaagttctt tgtcatttat
    36001 tacggctata ctttctaaat acggcacttc ttcaaaatct tccgtcgcat agtttgctga
    36061 acgaatttgt gggcttaaca cctctccttg tccatacaca gcagcttgca taaatggata
    36121 atagatcgtc tgtctccaag cttctccgtt tgtttctgtc ctgattggtg caataacgtt
    36181 taccaattga gcaagacaag caatctttac tctatctgca tgtttcaata aagtgattaa
    36241 tagacagcct aatagtaatg catcttcaaa attatagtta tcttctagta aaggaggagc
    36301 aatctgccaa ggcgctgctt gctcatcttg ttccgctgaa tgataccaaa tattccattc
    36361 atcaaaggac aggttgatct gcttcttact atgtttcttc gccttgactg tatcgcacat
    36421 cgctgtcact tcagaaatga accgatccat gtctaaggaa cgtgcaagat aatgagaaag
    36481 atcatttttt tggttgccat aatattgatg cagagacaaa taatctattt gatcgtacgc
    36541 ttgatcaaga actgttaact cccaatcacc aaatgtaggc atttgccgat tcgaacttcc
    36601 gcataaaacc acctcaattg aatcatctac gagtttcatc gccttagccg tttcctctgc
    36661 taaccgtcca tattcttccg ctgttttatg tccaatctgc caaggaccgt ccatttcatt
    36721 ccctaaacac catgtcttaa ttccaaaagg gctctcttga ccattcgaac gccttaaatc
    36781 actccaataa gttcctttag gaaaattaca atattcgact aaattacgag ctgcatctac
    36841 tccacgagta cccagattga cagccatatt gatttcggcc cccacatgct gacaccagtt
    36901 ataaaattca tgaatcccaa cttcattcgt ttcaattgtc cgccaagcta aatccaacct
    36961 tctaggtcta tttgctatgg gcccaatact gtcttcccaa cgaaatcctg acacaaaatt
    37021 tccaccagga tatctaatca tgggaatgtt caattctttt accaagtgaa tgacgtcttg
    37081 gcgaaatcca cttttattag ctgatggatg atctggctga taaatccctt catatactgc
    37141 tcgtcccata tgttcaataa atgaactgta caaccgcgga tcaactttgc ctacacgctt
    37201 gacactacta atcaacattg tggcttccat aactgacctc ctttaattta tacatgaata
    37261 atacacctcg ttg
//
LOCUS       DANKAZ010000019        34688 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000019 DANKAZ010000000
VERSION     DANKAZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            322..480
                     /locus_tag="PWY67_000895"
     CDS             322..480
                     /locus_tag="PWY67_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2057200.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            552..1778
                     /locus_tag="PWY67_000896"
     CDS             552..1778
                     /locus_tag="PWY67_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2057201.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(1866..2258)
                     /gene="rpsI"
                     /locus_tag="PWY67_000897"
     CDS             complement(1866..2258)
                     /gene="rpsI"
                     /locus_tag="PWY67_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2057202.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2274..2717)
                     /gene="rplM"
                     /locus_tag="PWY67_000898"
     CDS             complement(2274..2717)
                     /gene="rplM"
                     /locus_tag="PWY67_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL2057203.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3236..3703)
                     /locus_tag="PWY67_000899"
     CDS             complement(3236..3703)
                     /locus_tag="PWY67_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2057204.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3823..5361)
                     /locus_tag="PWY67_000900"
     CDS             complement(3823..5361)
                     /locus_tag="PWY67_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL2057205.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5441..6127
                     /locus_tag="PWY67_000901"
     CDS             5441..6127
                     /locus_tag="PWY67_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL2057206.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6328..6855)
                     /gene="msrA"
                     /locus_tag="PWY67_000902"
     CDS             complement(6328..6855)
                     /gene="msrA"
                     /locus_tag="PWY67_000902"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2057207.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6939..7616)
                     /gene="pgmB"
                     /locus_tag="PWY67_000903"
     CDS             complement(6939..7616)
                     /gene="pgmB"
                     /locus_tag="PWY67_000903"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2057208.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7613..8743)
                     /locus_tag="PWY67_000904"
     CDS             complement(7613..8743)
                     /locus_tag="PWY67_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2057209.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8743..10926)
                     /locus_tag="PWY67_000905"
     CDS             complement(8743..10926)
                     /locus_tag="PWY67_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2057210.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10923..11948)
                     /locus_tag="PWY67_000906"
     CDS             complement(10923..11948)
                     /locus_tag="PWY67_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2057211.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11993..12820)
                     /locus_tag="PWY67_000907"
     CDS             complement(11993..12820)
                     /locus_tag="PWY67_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2057212.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12817..13884)
                     /locus_tag="PWY67_000908"
     CDS             complement(12817..13884)
                     /locus_tag="PWY67_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL2057213.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13910..14746)
                     /locus_tag="PWY67_000909"
     CDS             complement(13910..14746)
                     /locus_tag="PWY67_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2057214.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14743..15612)
                     /locus_tag="PWY67_000910"
     CDS             complement(14743..15612)
                     /locus_tag="PWY67_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2057215.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15682..16965)
                     /locus_tag="PWY67_000911"
     CDS             complement(15682..16965)
                     /locus_tag="PWY67_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2057216.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16979..18664)
                     /locus_tag="PWY67_000912"
     CDS             complement(16979..18664)
                     /locus_tag="PWY67_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL2057217.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18939..19964
                     /locus_tag="PWY67_000913"
     CDS             18939..19964
                     /locus_tag="PWY67_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2057218.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20559..24233)
                     /gene="rpoC"
                     /locus_tag="PWY67_000914"
     CDS             complement(20559..24233)
                     /gene="rpoC"
                     /locus_tag="PWY67_000914"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL2057219.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(24291..27908)
                     /gene="rpoB"
                     /locus_tag="PWY67_000915"
     CDS             complement(24291..27908)
                     /gene="rpoB"
                     /locus_tag="PWY67_000915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2057220.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28636..29604
                     /locus_tag="PWY67_000916"
     CDS             28636..29604
                     /locus_tag="PWY67_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2057221.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29622..30527
                     /locus_tag="PWY67_000917"
     CDS             29622..30527
                     /locus_tag="PWY67_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057222.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30524..31330
                     /locus_tag="PWY67_000918"
     CDS             30524..31330
                     /locus_tag="PWY67_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2057223.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31466..32455
                     /locus_tag="PWY67_000919"
     CDS             31466..32455
                     /locus_tag="PWY67_000919"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL2057224.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32772..33296)
                     /locus_tag="PWY67_000920"
     CDS             complement(32772..33296)
                     /locus_tag="PWY67_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL2057225.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33583..>34688
                     /gene="scm"
                     /locus_tag="PWY67_000921"
     CDS             33583..>34688
                     /gene="scm"
                     /locus_tag="PWY67_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2057226.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTS"
BASE COUNT         9938 a         7343 c         6486 g        10921 t
ORIGIN      
        1 atacatgttt atcgttatta atagtgaata ggaaaaaaac tatttttgat cattgacaat
       61 taaatacaat ttttgaataa cgatccctgc tattcgagct agaaactcaa gtacgccatg
      121 acctgattag cgacaaatac ttagagattt ctttttgttc tgggaaaaca aaatgccatg
      181 acaatagcag caaataatga tacttccgta aggctcgccg taaactgggg agaggtgaaa
      241 ttcctatgtg cttacccaaa gcaattcaaa aatttcataa ctactatttt aggagatgat
      301 tttatagaat ccagagtcta cctgaataaa aaacaaacct gtgcatttct aggaatcgat
      361 aataatactc tagataagtg gattcgtata tataatttcc cagtaataag agttggtggt
      421 gtagttcgct ttgacgtaga agatattaaa gactttatga atacatttaa aataacttga
      481 aattttcttc tcgccgcata tactaaaatt agtgaaatat actgccttac tttttttaag
      541 gagtgaggat tttgcctaca aagctttcaa atgggaaata taaaactaat ctgagatatc
      601 caaaaaggtt taaagaaata acaggaattg catcagaaaa gtatcaaaaa acatttccaa
      661 ataggcaatt ggcgattaaa gccgaaaatg atatgaagaa gaaaatagaa aaagttttga
      721 gagaggagaa tgcaaactca ttagaattaa aaggaaaaat aacgtttaaa aaattttacg
      781 aatctaaatg gcttcctaga tatgaattag gtcaaacaat tagatcaaac aggcctccta
      841 gtgatataac cattagtaat acaaaagata tatttcggtt gcatatttta cctatgtttg
      901 gtgaatacgc aatgaattat ctaaatatca atactgagat aatatcagat gaattaacca
      961 aaaaatcgaa agaatatgca aatataaaaa tcataaaagg atatgttcgt tccatgttcg
     1021 atattgcaga aatactaaat tatatcgaat ttaatcgaac tactaaaatt attcaaagta
     1081 tcactgcacc taaaaaaaat gctttagaag aaaaaagaat acaagaaggt aagcaagcac
     1141 tatcatcaaa agaacttact aattggatag aagcagtaaa tgatgatttt aataatcatt
     1201 tacttacttt tcatgattac acacttttta tgattaccct gtatcttggt gatagaaaaa
     1261 gtgagactta cgctttgcaa tggaaatata ttgattttga aaaacaaacg gtccgtctta
     1321 aacatacttt agataaatat caaagaaaaa agtttacaaa aggtcgaaaa gataccgtca
     1381 ttcaagtacc agaagttgta atgacattgc taagtgaatg gaaatcggta caagctgatc
     1441 aattgttgaa attaaaaatt aagcaaacgt tagatcagta tctctttact tatactaaac
     1501 catctggaga agtaaattgt cctgttcatg ccgattacct aaactatcga atcaattcaa
     1561 ttaaaagaag gcatcctgac ttagttcatt tatctccaca caaactccgt catacttatg
     1621 caactatagc tagacaaggt ggagcagata tgaatcaaat ttcaaatgct ttaacacact
     1681 cagatatttc tacaacaaag atatatgtga acacaccaga tatagttgat aaggctgttt
     1741 ttgaagcttt tcagagagga ttgaataagt gtgattgata tgtgttctta aatgtgttct
     1801 taaaaacaaa agaagcactt tccaaataat tgaaaagtgc cagaaacgtt gatatataaa
     1861 ggatattaac gttttgagaa ttgtgaagct ttacgtgctt tcttaagacc tggtttttta
     1921 cgttcgacca tacgtgcgtc acgagtaagt aagccagcac gtttaagagc taaacggaag
     1981 tcaggatcaa cttctagcaa tgcacgagcg ataccatgac ggatagctcc tgattgtcca
     2041 gcgtatcctc cgccgtttac gttaacaaat acgtcatatg cgccttttgt ttcagtaaca
     2101 gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg gaatgtattc ttcaacatct
     2161 tttttattta cagtaatttt gccagttcct ggtactagac gtacgcgagc aactgcgttt
     2221 ttacgacggc ctgtgccgat atattgaact tgtgccaatg aaattccctc ctattagatt
     2281 aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat gtggatgttc tgctccaccg
     2341 tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt ttttaggaag catacctttt
     2401 acagaagttt cgatcaaacg acgagaattt ttagcacgca attcgccagc agtgattgat
     2461 ttcaatcctc ctgggtagtt gctgtgacgg taataaattt tgtcagtcgc ttttttacct
     2521 gttaatttca ctttgtctgc attgatgacg atgacgaaat cgccagtatc caaatgaggt
     2581 gtgaaagttg gtttattttt accacgtagt acagatgcaa caactgttga aagacgtccc
     2641 aatggaacat cagttgcgtc tactacatac catttacgtt ctacttcgcc tggtttggcc
     2701 atatatgttg tacgcacgtt attttcctcc aatttaagtt ctgatgtttg acatacactt
     2761 cgagtttccg gggctctttg tggggcaaac aataccattt aacatattac ccgctaatct
     2821 agtcaatgtc aacgtttttc tgcttgattg aaaaatttat atgaaacttt acttcttttc
     2881 tttcttttat ataaaagggt cgtttcctgt caaaatgaaa aacaaaaaga ggctgtgaca
     2941 aaagtcttgt cacaacccct tattttgaat aaacggtgtt ctcctgccaa ctcctcggtc
     3001 acaagtcaca atatccataa aacataagaa gctgtttttt aaaattgttt cttgtgactc
     3061 ggagctgaac ggcaggctta caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg
     3121 tattagctca aaaaacggca aaatatgagg aactattttg acgtttgttt ctctaatact
     3181 caggtcaaaa cgctgatttc acaacctctt tttcggtcaa gttcaacaac agtttttacg
     3241 cgtcgatctc tggcgctttg cccaaatgag cttggatcat ccagatacgt ttttctgtgg
     3301 ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc gccttcttct ccagaaactt
     3361 cgattccttt ttggtaaaga tctgctagat aacgatagcc tgcaacaagt gtttccaaac
     3421 gttctggaat agtacgatcc cagcggccaa tttcatcagg gatttttgtg ttatctgcaa
     3481 attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat caagcgttcg gatacttcat
     3541 ctaactgagc gttcaaatca tccatgaact catccatcat tgggtgcaat gtaaggaaac
     3601 ctggtcctct catgtaccaa tgtgtttggt gaacaacaac agacatttgg cttagatctg
     3661 caactaattg atttaatact tctttggttt gttggaattt catctttctt gcctcctaac
     3721 attttcttac acttaaaata atagtagact ttcaatgaaa agtacaacaa aatcatttac
     3781 ctgagaacga ttttcagttc gcttttttat ccgtaaagcc aatcattttt cagaatcagc
     3841 taattcttct cgcagttgag caaattgctg ataagtctgt gctaaatgct tttgtgtcga
     3901 aacaaataat ggatagattt tctgatattt tttatgatca gtttcttgta ctccaacttg
     3961 ctctttcaat tctatcaatt cctgtgcttc actgatactt tggatctttc cgatgcttct
     4021 ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg acagtaggga aaacaagcgg
     4081 gtaacccagt acatcagcca tcatctgacg aaaaatcgga gattgagcaa acccgcctgt
     4141 agcacggatc tctgtgagct gattttcctg gcaaactcct tttaaaatcc gataaaggtt
     4201 gagacagatc ccttctagaa tcgccttcat catttctttt tctgtatgtt gccgctgtaa
     4261 accgataaaa ctgccaaacg catctgcgtc ccaaagcggt gctcgctcac caagtaaata
     4321 gggatgaaaa atcagaccat tggcacccgc tggcacaaaa gcgatagccg aaagcagtgc
     4381 atcatatgga ttttctcctt tttctttagc agattgaacg acttcctgta aaaatgtatg
     4441 ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct cctcccacta cccaatgttt
     4501 ctgatccaga acataacaaa acgtctccat ttctggatgg aactgaggct gagtagtaat
     4561 aaaacgaagt gctccgctcg ttcctacggt caaagcggcg atcgtatcac caaaagcacc
     4621 taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa acaaatgata tatcatttgg
     4681 caaacctagt tctgctgctg tttttgatgg tagacctgtt acttgatagg tggaaggaaa
     4741 gacttgcggc aattgctcta ttcgaatgtt caagtagtca agcacttctt ctgcccattg
     4801 gaattcatgg atatcaaaat atccagttcc tgaagctgtt ccgtagtcgc tgatcagttc
     4861 gccaaacaat ctataaaaga tatagtcttt gataccgaca aaataagcag tttttttgaa
     4921 taattcaggt tggctgtttt gcagccactt gattttgttc aatggtgtca ttggatgaat
     4981 cggcgtccca gtctgccgat aaaattcttt gccaagcacg gtatatttca gtttttctgc
     5041 ttcgtccgct gctcggttat ctgcccaagt gatcattcga gataacggtg ttttctcttc
     5101 atccaacaat atcaggccat gcatcgcaga ggagaaggag acaaattgaa tgattgtgtc
     5161 tgaaggcaac tgtttaatta cttcttttat aacggtgcag acaccttgaa agatcaattc
     5221 ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa gggtagcgaa aataagcgga
     5281 tgctgcttct tgggcttcat ctgtgaaagc aactgctttg gtttgtgtgg ttccgatatc
     5341 taatccgatt gaaacgttca ttttttcacc tcaaatttta tgtatgtaca aaaatttagt
     5401 ttaagcttgc gtatcttggt tttgaaaagg gggagatggc gtgttacttt attttatcat
     5461 tggttgctgt atcggatcat ttttatgttt aaccgcccag cggattccgt taggtcactc
     5521 tattatttat cctagatctc attgtgtcaa atgctgtcgt tcgctttctt ggtatgaatt
     5581 gattccgatt ctctcgatca tcgtccaacg atttcgctgc cgctattgca ggtgtcggtt
     5641 gcccttctac tatttgttgg cagaagcctt atgtggtggt ctttttgcct ggttctttac
     5701 tttttcttcc gcaagaaatt tttcgacgtt catatggatg ctttcggctt tgctattttc
     5761 gttgatggat ttattttact tcatggtcca ttctcacacg ctattctgtt cttgggcaat
     5821 tttatggatt ttttggttgc aggccggcgt cttccaatgg caaagtgtgc tgttaacgtt
     5881 tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat ctcggagctg gcgatactct
     5941 gttgatgctt tcttggagtg gtggcttgtc tctagaagag ttgcttcaga tcttgtttct
     6001 tgctagtctg tttggaatat ccgttttttc tgtttacttt atctgtcata aaaaaaagtt
     6061 ggaaaagctg cctttcattc cttttttgag cagtgcgttg ctgattgtcc ttcatcttag
     6121 gtattgaaaa aaggttgtga caaacgactt gtcacaacct ctgatatata gtgttctctt
     6181 accaaatcct tgtgacttgg agctaggcga caggttcaca acctctccac taaatggtgt
     6241 tcaatcagct gaccctcagt attagctcaa aatgctggtt ttacaacctc tgatatacgg
     6301 tgttccataa gtagcttttt cggcttctta tttcttgacg ccccaatgtc gacgaaggaa
     6361 acctgcacgt ccagaacctt ctcgataaag attgtaatgg agcttgtttt tcttgtaata
     6421 atcctgatgg taatcttctg caggataaaa ttcgcttctt ggttcgattt ttgtcacgat
     6481 tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt gaacgctctg caatatttcg
     6541 ttcttcttca tcattatagt agatcactgg acgataagaa tcaccgcgat cagcgaactg
     6601 tcctaatgcg tcagtcggat cggtttgctg ccaatagatt tccaccaact gatcataaga
     6661 aataacttct ggatcatatt cgatctctac tgcttctgta tgccctgttg tgcctgttgt
     6721 tacttgttca taagttggat ttggtacatg cccgccagta taaccggatg tcacagaaat
     6781 gatccctggc tgctcttcaa atggtttgac catacaccaa aagcatccgc ctgcgaaaac
     6841 tgcttttttt gtcatataaa atcccccttt tttctcatca taccttgatt tccaaaagga
     6901 ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt atatacgtga tacggcttct
     6961 gataaatgcc aaaacgaacg tacttgatca aaaacggtta aatcaggaat cacatattca
     7021 gcagaaggaa gatttatgtt ttcacctatt cccaatacat gcatgttacc agccaaagct
     7081 gcttgacaac cagcttctga atcttcaatg accaagcatg catttggcgg tacattcaat
     7141 tgctgtgctc ctttgagaaa aacttctgga tcaggttttg cttttgatac ttgcgtgccg
     7201 tcaataagga catcaaaata acttgtcaaa ttcgtttttt ctaaaatgag gcgtgcattt
     7261 ttgcttgctg atcccaatcc gatcttgatg tttgtttttt tcaagtactc aagaacagct
     7321 attgcaccag gcaaaacgga tgtttcatcc attttttgaa tcgcttgaag atatagattg
     7381 tttttttgct cagcaaaggc tgatttctct ttttctgaga atttctgttc catttttcca
     7441 aatgacagca atcgctctag tgactcggtt ctggatattc ctttcagttg ctcgttttct
     7501 ttctcagtga atggaatgga cagctggtta gctaaaacca gccacgcttc gtaatgataa
     7561 cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg ctgctttgat tttcatcgat
     7621 tacctccttg aaaatgaacg acttgaagta aagatgcgat gtttttagct gtgcgatgaa
     7681 gattatcagt ggcttgctgt aaagcttctt gtaaaggcac agctgcagga aggatcggaa
     7741 aaacggccga aatgccaaca tctgctgctt tttcgcttcc tttaccaaca cttccgcaaa
     7801 tagctatcac aggaacgccc tctggtactc ttctagcaat cccaactggc gcttttcctt
     7861 ttagactttg tccatctaga cgtccttcgc ctacgattac gaggtctgct tgctgtaccc
     7921 gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc agagactatt ttccctgaag
     7981 caaatgctaa cagtcctgca cccattccac caccagcact actgcccgat gcctcgatta
     8041 cttgaggggc aaatctcata taaaaatcat gcatggcttg gtcagccact gctaattcat
     8101 tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc gccttctttt ccgcataaaa
     8161 gattagtaac atcagtaatg acagtgattt tggctttaat cagctcaata ggcacgttcg
     8221 ttttatcaac tgtagcaatt ttacctagat tttctcccag tgcttctact ttttgattat
     8281 cttgatcgta aaaagcataa cctagacctt ctgccatgcc gatgccgcca tcgttggtac
     8341 tacttccgcc aacgccgatg atgatgtgtc gaacaccttg ttgcaacaat tgtcgtatca
     8401 gttctcctac tcctcgcgtg gataacctaa gtggctgttg ttggtttagt gggacatctt
     8461 tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg accgtctgaa gcgtactgga
     8521 tttctttgtt tttgccaaat gcattagtaa cgactgctgt ttgccgcttc aaccttaaac
     8581 tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc tataggtagc agatcatatt
     8641 cagcctgaac aaaaacttct tgaaaacctg attgaatggc ttgtgctgcc tctaaggctg
     8701 tcagactttc tttgaaagaa tctggcgcaa tcagaatacg cattattttc ccctccgttc
     8761 caagatcagt tctttttcca gctggtattg gtctccattg acaaaaatag gtaatgtttc
     8821 tttcgtctct ttttttatta ctatttgttt ttttgtcaaa acaaatagca acctgtgatt
     8881 tttccaaata aaaggaaaag aaattttttc ccatgctgaa ggaagtcttg gagaaataga
     8941 aagagttcca ttcgttactg taatacctga aaaaccaaat ataactgcta gccatagtgc
     9001 tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc ggatctggtc ctaaatcaat
     9061 caggcacgct tcttggaata aatgatacgc ccattccatt tcttggatac ggcaagcctc
     9121 tatggcgtga atcgctttac tcaacgatga gtcgtggatt gttttgtttt catagtaatg
     9181 caaattctta gaaacgattg gttgagaaaa atactcaggt aataagtata acagcatgac
     9241 aagatctgct tgcttgctga cttggagttc atttacttcg ctacgggcat aatcaagtaa
     9301 aatagactgg ctgccaggac gttctttgta agtggtcaaa tcaataactg gcttactgag
     9361 atacgtgtca tcttgcggaa tcagctgttc tttgttaggc actggcaaat ataacttatc
     9421 taaaaaattt tgacattctt tcaaaaaaag cggatcgcca gcttgatatt taacagctaa
     9481 ggccacatta taatgcgcca gatagttcgt atatgcatta ttgtcgatat attctgtgta
     9541 ttcatctgga ccaatgactc tgtgtatctc taaatgatca tcgactttta cagctctgct
     9601 cagccaaaaa gctgctgttt catgaatgag cgtttttccc tttgtttcta aaaacgattg
     9661 atcctctgtt gcttggacat actcttgaac ggcataagca aggtctgcac tgatatgatg
     9721 ttctgcggca gctgaagcaa cttcttgtct tgttccggtt ttgatattca atgcagcaaa
     9781 tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat gggaaaagtg caccttgata
     9841 gtgataatct cttgcttttt gccgtgcgcc ttctaatcga tcataacggt acagcagcaa
     9901 attctttgct acttcaggaa actgatataa aaagaaaggc aggataaaaa tttccgtatc
     9961 ccagaaaaca tgccctttat aaccttcgcc agttagtccc ttcgccccta cactaacact
    10021 tggatcattg cggggagtca taatctgtaa atggtaagca gcgaagtcca tcgctaactg
    10081 atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga tgccaaaaat ctgtccaata
    10141 agacatcgac tgctctaaaa tttctgtata actaacgctt ttctgggctt gggcaatact
    10201 tttcttaaac gggtctgttt gctgaagaga ggtagagagc acgatgatct tctccagcgt
    10261 aaaggacgta ttttttctga tcttttctga aaaggaagcg gtgatccggc gattttttgc
    10321 ttggaataca cctgctttat tgaatttagc tgcaatccca atatcaattt tactttctgt
    10381 tgtctggtag gatgcactga tcaaatcgtc accaaatact cgcagttctt tttcaaccaa
    10441 ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc gttttcacat ctacttggca
    10501 gtcttcggtc aacggcttga tgaccacttt cagggctgcc tgatggagat tattttgatc
    10561 agcaacacga caagtaatga cttccacttt ttttccttga gcagtttcaa tgagcgcata
    10621 gcgtttaagc tctcccattt ctaaatctag tgcccgctcg tatgcaagaa ctttcatcgt
    10681 ctcaagagaa aaatgttcac cgtcgatttt tatttcccat ctgatgacat ctgggagatt
    10741 cactaactcg gctgattctg ctcctaaagg tcggttatat acaccagctg caaacattcc
    10801 ccttacttga ttccgatagt cttcctcctg gcttcctctg ataccaaaaa atccatttcc
    10861 aatggtcatc tgactcgcat acttattgat atactctaaa tcaaactgtt tttcttctac
    10921 tttcataagg taataacttc tcctgtttct gccgaacgat ataaattttc caccagattc
    10981 tggatgacat atccttgcca gccatctgca atcatctgag tctggtcacc gagcgcccga
    11041 ttcacaaaat gagccatact tagttcatgt gggtcattaa ctggcgtggt cttttgtaag
    11101 gaaaccagct ttccatctac atcttcgaat agttcgcctg gatacagatt agcacctgcc
    11161 tgatcgccac aaaaagccac attcagtttg tcttctctga tattcaaagc aaacgaagta
    11221 ttcagttgga tgatcttgcc gttttctaat tccagtgtgc caaaaattgc atcttctact
    11281 gtgaatttgc ttggatccca tttcccgaat ttccctgaat tttttttcgt accaattttt
    11341 tggaagctgt acgcccgaac gctttttact ttaggaaatc cagtaatata catcgctgta
    11401 tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc cttgaagttc tttgtcgata
    11461 aacgttcccc agcctggaac accagaccga cgaagcgcat tcgcttctac gtaataaata
    11521 tccccgagaa catcaagatg attcttgatc attgttgctt cgtcagaaaa acgatgctgg
    11581 aatccataag ccaaaatctg cccgttttta tcggctgtct ctgccatctt tcttgcttga
    11641 tcagctgtca tagctggtgg tttctcgcac atcactgaag cattagcttc gagggcagcc
    11701 aatacatgtt cgaagtgaaa gcgattgggt gtacagacac tcacgatatc gggagaaatc
    11761 tcattcagca tttgtgtaac tgatgtatac gcatgagaaa atcctgtacg tcggcaaaag
    11821 tcttctgctc gctggctgac tggatcaacc acagcggcca attctaagac atctgatctt
    11881 ttttgataat aagcagcatg tactttttct gctacttgtc cagcgcctat gattgctact
    11941 ttcttcattt atttctctct cctttgctta tttacttatt gttattcttt ctctattctt
    12001 tctctaaatc atttaaatga cgcgctgttt ccagacagtt tttcaagtat tgcaatgatt
    12061 cctgataaat tttcacttca ttttctcctc gcaaacgaca ttcatatacc atataacctt
    12121 catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa atcaattgcg ccagatcctg
    12181 gttggtagcg atgattatct gctaaatgga tatgacccac atactctttt tgcgtagtca
    12241 atgcttttga aatatcatct tcttcaatat tcatatgata aaaatcagcg atgatctgca
    12301 catgtttcaa ttgattagag tcaatgtaat gacgcgcatc cgctaacaaa ttgatcatat
    12361 ggtcttggta tcggttcaaa ggctccaaat aaataaccgt acctgtttct tcggctactt
    12421 tatctaaata acgaagtgat tcgctgactg ctttatagtc tccttctttg cttcggggtg
    12481 aaaccatcgg cggtaatcga taagtgaaca ttccccaagc tgctgggata acgattcctt
    12541 tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat ttgacgtaca ccattaagac
    12601 gccgttcttc gataaagtca ccgatccacc catcgtagcc gttacaagct gtcactactg
    12661 ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat attttctatt aacaacttac
    12721 cgtcgatttc gtattcttca aatccacagt ctttgataaa tctaaatttt tctaaaatgt
    12781 ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat tgttctcctc ctcggacttg
    12841 aacgcccatt ttgatacttt cttctgggtg ctggtctaca taacgcatgt aagcttctgc
    12901 gctttctgca aatggcacaa ctggatcaat gatttcttca caatccaagt aaccgttcat
    12961 caataattcc caacatgtgt cctcgattcg tttacggttc catcttgggt aatctgggtt
    13021 tggttcacta gatgctcgag caaagacgat ttttgcatta ttgaaatgtg cttcgcgacc
    13081 aagattaaac ccagctggaa aaggtttagc aaaggcaaca taagaaatga tcccgccata
    13141 gccgatccct attaaagaag cttgtaatgc ggcagcactg ccactcgttt caataactga
    13201 atctactcca cgtttatctg tcaattcctt tagacgaaaa ccgacatctg ctttcatagg
    13261 atctagcgtg taatcggcac cgtgtttttc tgctaattct ctacgctgcg ctatgggatc
    13321 gatcccaacg atggtagtag cacctgcttt tttagctaat tgaatcgcaa tttgcccgat
    13381 agcgcctaaa ccgaagattg ctacagcatc acctgctcga acatgtgctt cccggatacc
    13441 acctaaagcg aattgagcag gatcgtaaca tactgcattt ttccattgat ccaaccgatc
    13501 aagtttacgt agtttataat tatcaacacc gtttgtaatc agcgtttcac gaatgggtcc
    13561 ataagtgcag actaactctc ctttttgata ctctgtcacc aaactgcccg tttcttcgat
    13621 cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat accacaccgc gttggcttcc
    13681 ttcttctctt ggcaagaata ggcgccattc ttctgaaaat tcttcatcga taaatggact
    13741 taatcctcta aagtcgacaa tttctgttcc tggttttgga gtagcgaaaa ttactcggat
    13801 acgtacttcg ttcttttcga tctcacggtc ttcgtaatct ttgaatccgg ctattcttgg
    13861 ttctaatgca actaattttt tcatcttttc tttcctcttt cttttctttt tatcctttta
    13921 cgccgccttc gactaaatca cctttaataa atcgttcaga taacgcatac ataatgatga
    13981 ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc ccaaatgtag tctggtgtac
    14041 tgaataatgt gttcaaaccg attggtaaag taaatttttc ggaggtcgac aggaaaatcg
    14101 aagcaaataa ataatcattc cacgaaacca taaagcaata aacgaagaca gaaatgattc
    14161 cagaaatagc caatggtata atgatccgaa taatgatctg gatccggcta agaccatcga
    14221 tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa ataacttttt agcatgaata
    14281 tcgcagtagg aagtgtttga acgaccatcg tgataatcag tgctgtattg gtatcataca
    14341 aacctagacc ggaaatgatt ttaaataatg gaacaacgat caaaataccg gaaaacatat
    14401 aaacggtgta aaagctagca ttgattttcg agcgagcttt gaagcgtagt tttgacaaag
    14461 cgtatgcccc gctgatccca atcactactg cagttacgga ggctatcaag gagatcacta
    14521 aactattttt gaaatacatg atgaatggaa aaatctcagc attgaaaata tcctgataat
    14581 gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt tgcaattgct tcttgtgaat
    14641 ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa attgacagct aatagcaaca
    14701 aaatcgtcgc gtaaaataag attttcctgc tctttttatt tttcatttca agtccacctt
    14761 ctttcgtgag agcaagacga ccgctgcaat aatgatgaac aatagaatcg atatagctgc
    14821 tgcttttcca agatcattga acgcaaaggc tgtctgatac aaataaacgc ctaagatatt
    14881 gactttattc gtcagtagat agacatctgt aaacatatag aacatccaga tagatcgtaa
    14941 aacaatcaca gttgctagta ctggtttgat tgccggcagt gtcacgatct tgaacttctg
    15001 ccaagcattt gcaccgtcca tttcagctgc ttcataaagc gttttgtcga ctgtttgcaa
    15061 aatagccaaa aatgaaataa acgcatacgg atagtatctc cagatagcaa acaaaacgac
    15121 cataaagaag ctagcaactg gctggtcgaa ccacagcggt gcttcgttga aaagctgcaa
    15181 cctatctact aacagatcat tgacgattcc ataactatta ttgaacatat atttccaagc
    15241 aaaaatcaaa gaaatagatg gcgtaacata acttaataaa atcagtgaac gcaccgtttt
    15301 cctaaatctg aacttgcggt taagcaaaat agctacggct aatcctaaag ctgtactgcc
    15361 tgctacaact aagaatgtgt aagcaactgt taaacctaaa gaacgataaa attcactatc
    15421 acttaaaatt tcaagataat tgcttaatcc gataaaaacg gcgtctgagt taggattcag
    15481 cggcatatcg aaaaaactga tctgaatatt ggaaagcatt ggaatcagca ctaaaacagc
    15541 taataaaagc atgcttggtg ctaaaagtcc catggctaat tttaagtcag aatgtttcct
    15601 tggacgattc attgaaacct ccccctgata atctgccaat agctatctac ttttttcgaa
    15661 caccattggc agaacttctt gttatttcgt atttttttgt gcgtcagcta aagcttgatc
    15721 gacatcttcg tttccgacag ttacttggtt cactgctttt ccgatcacac ctgagcttgt
    15781 gatatctccc attttcgtga aattcttttc cccgactagt ccaaagactt gtacttcatc
    15841 aaaagcacta gcaatctcag atggcagctc gccaaaagac ttgatgactt cattttcttt
    15901 gtaaccatcc agttcgacta cttgtttatt cactggttga gcaccaccag gagacatcaa
    15961 gacccacttt tccatgtttt tcggttgaga taagaaagct gtgaatgctt cagcagcctc
    16021 tttttgtttc tcgtctagac cagaagaaat cgtcaatcca gatactgtgc catagacagc
    16081 ttgatctttt tgtgttggga tagcaaaacc gatattttct gagttccccg cttcataaac
    16141 agaaggcaaa atgtaagttg aatagattgc cattggtgct gttccattca tgaaagcatc
    16201 tttgatttct gtcacatcat tagagcctgg catcgtgtat tgggataagt tttgataaaa
    16261 cgacaatgct tctctcatct tttccgtatc gatcgttacc tcgccttcat catccaaaac
    16321 atttgctcca tttgacaaag caaattgaga gaacgcttgc tcactcattg ttccttcagc
    16381 agtaggcata gcaattccat attgtttatt ctctttatct gtgaaatgtt gagcaatttt
    16441 taaaatatca tcccaatttt ctggttctga aaaaccagct tctgataatt tttctttgtc
    16501 ataccaaata ccttgaaccc agccgctaat tggtgcagca atatagcttt ttccatcttc
    16561 tgaacgaaca aggtttttgg ctccgtcata ataattatct tctccagctt cctcgataac
    16621 gttttggact gccgtctgat caatcagttc atctttatcc atcactttag caaaatcctg
    16681 actgacttcc attacttctg gtaatttacc ggaacgagct aacgtaacga tttttgtatt
    16741 gaacgctgat tcttctaccg gcacttgctt gattttgatg tctggatttt ctttttcaaa
    16801 atcagcaacc agttcattga tcacacttaa tcgttcctgt tcgacagaag agtgcatgaa
    16861 ttgaatcgtt gtctgttgtg attcgttaga gctttgactt tcaccacaag cagtaagaag
    16921 aaatccagta gctagcagac aggtagtaac aataattttt ttcatttttt tccacctctt
    16981 attttattat tttcagccac atatattcat acggctggaa tatcaacttt cgttcttgac
    17041 tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc tccttttggt aaaagataac
    17101 ctgctgattg attggaaaaa ttatggataa ctaagacttt ctcttccttc gcttcgcgaa
    17161 taaaactgat aaaagaatct gctgagtcca gtaagtaaat caaactattc ggatggaagg
    17221 ctttttcttt ttttcgaata gatagcatct gggtcaattc accatatact gctgttctta
    17281 atggatctgt ctgcaactct ttctcaatat ctgctaacaa aaatttttgc cgattgattt
    17341 ttcgattctc tcctgttttt tctacgcctt tgtaatcatt tcgacttccc agcacacttt
    17401 gaatataaat tgccggcacg cctggtaacg ttaccaaaaa tgaatgagca gctaaaaagc
    17461 gtttgcttct ccattccgtc gtatctgttg gagaacttaa cgcatctaga tacgtagcgt
    17521 ttatctcata tggactggtc gttccatctg ggtttttctt gtagtttact cgggcaccct
    17581 ctttcttcag ctgttccact aattcaagga tcgttttttc cggcagaata cctcgtaccg
    17641 gattcaatcc gataccgtca tgggaggcta aaaaattaaa aaaagttgtt tttttcgatg
    17701 cgaaatcgat ggtttttaac cattctttca acttacttgt attacccgaa cgaatcgctt
    17761 gaagggtcaa aggtggtagc gaaaactgat agaccatctg tgcttcattc tcgccattcc
    17821 caaaataact gatattatct ttgtgtggca catttgtctc agtgatcaat accgttcctg
    17881 gagctgcttc atcagcgatt gcacggaata attggatgat tttatgcgtt tctggcaggt
    17941 gtatacaaga agttcctagt tctttccata aaaatcccac agcgtccaag cgaataaatg
    18001 aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat tttcaataaa acttctggat
    18061 ttttaaaatt caagtctact tgatcttcgc taaaagtcgt ccatacgtgt ttttctttcc
    18121 cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct tggtctagtc acgctgcttg
    18181 tatctgtatc cagtggcaaa tcaataaaaa aatctttgta cgtatcattc aagtttaaat
    18241 attcctgaaa ccaagtactt tcggcagaca tatgattgca gacaaagtcg aacattaacc
    18301 gtgtttcgct tctcatttct tctatatctt cccatgtgcc caaacggtcg ttgattgcct
    18361 gataatttac aacggagaaa ccatcatcag atgtatatgg gaaaaatggc aagaaatgta
    18421 caactctaaa tgtatctgcc aaatactttt gataaaattt cttaaatgta cgcaaagttg
    18481 gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa gaaaatatct tcttcgtccc
    18541 aatgcagttt gcctgcctgg cctttcttct gctgataaac actgactttt tgttcaatca
    18601 tagaggcggt tttctcagac aaatctgagt cgcgataaat cgcttgtaaa agttttgtat
    18661 acatcggatt cccctttctt ttttgggagc gttcccaacg atttgatcat attatggaaa
    18721 acgcttactg tcaatagcat ttttttaaat aattttgata tacacaaaaa tacaaataag
    18781 aaagcttatg tatcaataat taatagcttc ttaaaaaaat atggtctctc atgatcatgc
    18841 aagctattcc taaattttca aaatcataga caaaaaagaa tgtctattgt actatggaat
    18901 tattctaaaa cgctcccaaa aaggaggtgc atataaaatt gaaacctact atttatgaca
    18961 ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag agtattaaat aaccagccaa
    19021 atatctctga taaagcaaaa gcaagtgtac ttcaagcaat caaagaattg aattatgaac
    19081 cgagcaaaat tgcaagagga ctgtcaggaa atggttttga cgcgattcta gcattatctc
    19141 atcgtacttc tcatacgacc tatggcaatc cttttttttc agatgttatt caatccattt
    19201 ctgccgtttc tgaacaaaat aacttcgact tgattctaca aacggcaaaa acaccagaag
    19261 aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat gatccgagga attattattt
    19321 taagctctac gatggatgaa acacttcttt ttgaattgga tcaatataat attcctattg
    19381 tggtaatcgg aaaagtcgga aacgactaca cgaacatcta ctcagtggac acggataatt
    19441 tccaggatag ttacgatttg gtggaaacac tgatcaagaa tggacaccgt catatcggct
    19501 gtctccatgc tccgacagaa gtttatgtat ccaatgatcg tctcaatggt taccgtcaat
    19561 gcttatttga tcaccaccta gatatccgta atgaatggat tatagacggc ggctttcgct
    19621 tagaagatag catggcagca atgaatgatt taatgaagct acgagaaaga ccaactgcga
    19681 ttttttctac cgatgcatta aaatcattga gtatttacaa agtggcagaa caatctggaa
    19741 agcagatacc tgatgatctt tcgatcgcag gattcagtga tcccttattg tcctccctat
    19801 tttcgccaca gttgactcgg atagacattc cgacaaatca gttaggaatc aaagccacag
    19861 aacttttatt ccaactaatc caccaccaat accctgaaga aaaaagaatc attctttcaa
    19921 ccgagattac tctcacagat tcgatccaac aacaagaaat gtagaacaga attataaaag
    19981 aacagaaaca ctgaggataa gtcagttact ctgttctttt ttcaatcact catccctcgt
    20041 taccccctcc cggtatttta gcgaattttt cttattttcc atctgtttta gatagctgtg
    20101 gataactata atgtttgtgt taaactattt tttccacagg gaagaaaaaa ctatttttaa
    20161 ccacgttgtg tataagtttt tatagttcgt ttttttaaac acatttaccc ttatttcaat
    20221 ccatgttatt tggggatact tatctctaaa acataaatag aataacagac atgtctactg
    20281 gttttgtttt attcgaggaa ttattcaata atagctagca aaatattaaa aataaccgct
    20341 ctatcttccc atcaatttat tttgcattca aaaaatgatc tatctttact tttgttgatt
    20401 gacttctctt ggaagatgat cgttccgata ccttaattgg atacgaaccg atgttgttaa
    20461 gatcctttcc cacttttctt ctagttagac aaattatttt agacaaacca aaaaagaggt
    20521 tgtgacaata atttgtcaca acctcttttt taaaatatct attttgtttg attgttcatt
    20581 gcgtcttctg cagccatttg cgcttcaata tctgaaatac tgtagacatt ttcgcttgct
    20641 acaccgactt cttttggttc catgtcacga taacgttgca taccagtacc agctgggatg
    20701 atcttaccta taataacatt ttctttcaaa ccaagaagag gatctttctt accgcggatc
    20761 gcagcatccg tcaagacacg agttgtttct tggaatgatg cagcagataa gaaactattt
    20821 gtttctaatg atgcttttgt gatacctagt aagacaggtc gagaagttgc aggaacgcct
    20881 cctgagacta gcgtattgta gttttgatct ttaaagtctg cgatgtctaa taatgtacct
    20941 ggtaagatat ctgtatcacc tggatccatt acacgaactt tacgcagcat ttgacgaacc
    21001 attacttcga tatgcttgtc gccgatttct accccttgca tacggtaaac acgttgtact
    21061 tcacgaagca agtagttttc aacagataat acatcgcgaa cttgcaacaa ttgttttgga
    21121 tcgattgatc cttctgtcaa tggtgcacca cggtggatca tatcgccttc tgctactttc
    21181 atacgtgctg tataaggaac agtatacgta cgtgtatctg tttttccttt gattgttact
    21241 tctttggtac gtgttgcagg atcttctgag atatcgatga catcccctgt tacttctgtg
    21301 atcactgctt gccctttcgg attacgtgct tcaaagattt cttggacacg aggaagacct
    21361 tgagtgatat cgtctccggc aaccccacct gtatggaatg tacgcatcgt caactgagta
    21421 ccaggttcac cgattgattg agcggcgatc gtaccaactg cttctccgac ttcaacgtca
    21481 gagccagttg caaggttgcg tccgtaacaa tgtttgcaga caccatgttt tgtattacat
    21541 gtgaagacag aacggattgt cactgtttcg acacctgcat caacgatttg ttttgcgatg
    21601 tcttcggaaa tcaattggtt tcctgcaatg atcaattcat ttgtttcagg atgacgtact
    21661 tctttacggg tgtaacgtcc cactaaacga tcttctaatg attcgatgat ttcatttccc
    21721 tcgcggatgg cttggatttc aagacctcgg tcagtaccgc aatcttcttc acggatgatt
    21781 acatcttggg ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc agtcttcaag
    21841 gctgtatcgg tcatcccttt acgagccccg tgagttgaga taaacatttc caagacggat
    21901 agtccttcac ggaagttcga aatgattggc aattccatga ttcgaccatt aggagcggcc
    21961 atcaaaccac gcattccggc aagctgtgta aagttggaaa tgttaccacg agctccagaa
    22021 tcagacatca tgaagattgg atttttcgct tccaaacctt ccatcagttt ttgttggatg
    22081 ctgtcttttg cagcattcca aacagcaatg acacgttcat aacgttcgtc atctgtgatc
    22141 agaccacgac ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt ttcaatgatt
    22201 tcttgttttt cattcagtac actgatgtca gcgatcccta ctgtgatacc ggcatgtgtc
    22261 gaatgtttgt accctaagtc tttcatacgg tcaagcatct tagaagtttc tgttactttg
    22321 aatcgtttga agacttctgc gatgatgttt cctaagtttt tcttcttgaa cggtaatact
    22381 aattcttgct ctttgatatg tgcaggaata tcagtaccag cttcaacaaa gtatttgtcc
    22441 ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca gataagggaa ttcaggaggc
    22501 atgatctcgt tgaagatgat cttaccaact gttgtgatca tcgtacgttc tttttgccat
    22561 tcagtaaatg gtttttcacc taatgagtta gggttcacac cgatacgtga atgtaaatga
    22621 acatatccgt tgcgccatgc gataacggct tcgttttgat ctctgaagac cattccttcg
    22681 ccttcgcgtc cttcttcttc cattgtcaag tagtagttac ctagaaccat atcttgagaa
    22741 ggagtaacaa ctggtttacc atctttaggg ttcaagatgt tttgagcagc taacatcaac
    22801 atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt ggacagccat ttggtctcca
    22861 tcgaaatcgg cattataagc ttcacataca agtggatgca aacggattgc gcgtccttgt
    22921 actaaaactg gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc ccggttcagc
    22981 aatactgggt gttccttgat tacgtcttct aaaacatccc atacttcgtc ttcttgacgt
    23041 tcgattttac gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac aagctcacgc
    23101 atcacgaatg gtttgaataa ttcaatggcc atttcttttg gaagtccaca ttggtacatc
    23161 ttcaagaaag gtccaacaac gataacggaa cgacctgagt agtcgacacg tttacctagc
    23221 aagttttgac ggaaacgacc ttgtttccct ttcaacatat gagaaagaga tttcaatggg
    23281 cggttacctg gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa tgcatctact
    23341 gcttcttgca gcatacgttt ttcattttga acgatgattc caggtgcatt caagtctaac
    23401 aaacgtttca aacggttatt acggttgatt acacgacggt aaagatcatt caagtcactt
    23461 gttgcaaaac gtccaccttc taattgaacc attgggcgta aatctggtgg aataacaggg
    23521 ataacgtcca taaccatcca gcttggttga tttcctgaag cgcggaatgc ttctaaaata
    23581 tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa ttcttctttc
    23641 aattcggctg cttctgcatc tagatcaaca ttatcaagta attgtttaat cgcttctgca
    23701 cccatagcag cttggaattc ttgtccatat tgttcacgtt tttcgcgata ttcgcgttct
    23761 gtcaacaatt gttttttctc taaattcgta tttcctggtt cgatgactac atatgaagca
    23821 aaataaatga tttcttccaa agcgcgtggg ctcatgtcta atacaagacc catacgtgaa
    23881 ggaatccctt taaagtacca gatatgtgaa acaggagctg ctaattcaat atgtcccatg
    23941 cgttcacggc gaactttcga acgagtaact tccactccgc atcgatcaca aacgattcct
    24001 ttataacgga tacgtttgta ttttccgcag gcacattccc agtcttttgt aggaccaaaa
    24061 atccgctcac agaacaaccc ttcacgttca ggttttaagg tacgatagtt aatcgtctca
    24121 ggttttttta cttcaccgta agaccagctt ctgatttttt cgggagaagc caaacctatt
    24181 tgcatacttt cgaatttatt tacatcgatc aaaaggggtt ccctccattt catttaatgg
    24241 caaaccgcaa gacggctgat cttcgaccgc cttgcagctc atagaccgga ttagtctaaa
    24301 ttatcttctg cggtttcaaa gttttggatg acatcgtctt tttcatcttc cacttgttca
    24361 gcagctttct tttctaattc tttggcagtt tgctgttcgg caaattttgt caaagcatca
    24421 acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc tgtttcttcg
    24481 atatccaaga cacgcatgtc cagacctagt gattgcaatt ctttcaccaa tacgcggaat
    24541 gattccggta cacctggttt tggaattggt tcacccttga cgattgcttc gtatgttttc
    24601 acacgtccga ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt ataagccgca
    24661 ccgtaagctt ccagtgccca tacttccatt tcaccaaaac gttgtccacc aaattgagct
    24721 ttacctccca atggttgttg tgtaaccaat gagtaaggtc cgattgaacg tgcatgcaat
    24781 ttgtcatcga ccatgtgagc aagtttgatc atgtacatca caccgacaga cacacgtcca
    24841 tcaaatggtt cacctgtacg tccgtcgtac agaacagttt tcgcatcgct ggccataccg
    24901 gcttcacgaa ctgtttccca tacgtcttca tcacttgcac catcaaatac tggcgttgct
    24961 acgtggatac ctaactgacg tgcagccata cccaagtgta attccaatac ttgcccgatg
    25021 ttcatacgtg aaggcacccc taaaggattc agcatgatat cgattggtgt tccatcaggt
    25081 aggaatggca tatcttcttc cggcataata cgggatacaa cccctttatt tccgtgacga
    25141 ccggccattt tatccccttc atggatttta cgtttttgaa cgatataaac acgaactaac
    25201 atgtttacgc ctggtgacaa ttcatctcca gcttcacgag taaagatttt cacatcgtga
    25261 acgataccgc cgccgccatg tggtacacgt aatgatgtat cgcggacttc acgagctttt
    25321 tcgccaaaga tcgcatgcaa tagacgttct tctgcagata attctgttac cccttttggc
    25381 gtgactttcc ctacaagcaa atcgccatct tggacttctg caccaatacg gatgataccc
    25441 atttcgtcta aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg tgtgatttct
    25501 tcaggtccta attttgtatc gcgggcttct gattcatatt cttcaatatg gatagaagtg
    25561 tatacgtcat cttttaccaa acgacggctc atgatgatcg catcttcata gttgtaccct
    25621 tcccaagtca tgaaaccaac taaaacgttt tggcctagag ccatttcgcc ttgttccata
    25681 gatggtccgt ctgccagagt atcccctttt tcgactttct cacccaaatg aacgattgga
    25741 cgttggttgt agcttgttcc ggagtttgaa cgacggaatt ttgttacatc gtatttatct
    25801 aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat caacgtattc aacgacaccg
    25861 tcatgtttac ataacaaagc agctcctgag tcatgcgctg atttgtattc cataccagta
    25921 ccaacccatg gtgcttgagg attgatcaac ggcactgctt gacgctgcat gttggcaccc
    25981 atcaaggcac ggttggagtc atcgttttcc aagaaaggaa tacatgctgt cgcaacggca
    26041 actacttgtt taggtgatac gtccatgtag tctactttat caatagatac ttctaagttt
    26101 tcactttgag cacgagccat aacaacgtct tgagcaaatg ttccatcttc attcaatggg
    26161 ctgtttgctt gggctacgat gtaatggtct tcgatatcag ctgtcaagta gtcgatttgg
    26221 tcagtaacac gacctgtttc acggtctaca cgacgataag gtgtttcgat aaatccgaat
    26281 ttatttactt tcgcataact ggacaaacta ttgatcaacc cgatgtttgg gccttcagga
    26341 gtttcgatcg gacacatacg gccatagtgg gagtagtgaa cgtcacgaac ttcataaccg
    26401 gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa gacgacgttt gtgtgtcaat
    26461 tcgccaagag ggtttgtttg atccatgaac tgtgacaact gagaagaacc aaagaattct
    26521 ttgatacttg ccacaactgg acggatattg atcaattgtt gaggtgtcaa tgtttcagta
    26581 tcttggatcg acattctttc acgaaccaca cgttccatac gtgctagccc gatacggaat
    26641 tggttttgta aaagttcacc gactgaacgg atacgacgat ttcctaagtg gtcgatatca
    26701 tcgacattgc cgataccttc catcaagttc aagaaatagc tcattgaagc aatgatgtcg
    26761 gctggacgaa ccgtttttac agctgcttct ggatagccgt ttccgatcac gtttacttca
    26821 cgttcagggt ctcttggtga aaatactttg atgacttgga cagtcattgg atctgtgacc
    26881 acaccgtctt ctgatggata gtaagtcact gaatttaggc cattatcaat gaaaggagct
    26941 agtgtttcca tgacttggtg agtcagaact gttccttttt caacgatgat ctcgcctgtt
    27001 tctggatcaa ctaatgtttc agctaatgtc agattcaata gacgtgtttt caaatctaat
    27061 tttttgttta ctttgtaacg accaacgttt gctaagtcgt aacgttttgg atcgaagaaa
    27121 cgagcattca gcaagttacg tgagctgtct gctgtttttg gttcgcctgg acgcaaacgt
    27181 tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt atgcaagtct
    27241 ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat cgtatcatcg
    27301 gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt acggtcgata
    27361 cgtacataag agatgtcttt tgcatcagtt tccatttcta gccaagctcc acggttaggg
    27421 ataacagtag aaccgaatcc ttcttttccg tttttgtcga cttttccatg gaaatagaca
    27481 cctggtgaac ggactaactg agaaacgatt actcgctctg ccccgttgat gatgaatgtt
    27541 ccttgttctg tcattaatgg gaaatcaccg aagaatactt cttgtgcttt gatttcgcca
    27601 gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt tgcatcatgc
    27661 gcacgagctt ctgctactgt atactttggt tctttcaatt cataatctac aaattccaaa
    27721 gatagattgc cattgaagtc atcgattggc aaaatgtctt caaacatttc tctaagtccc
    27781 tcatctaaga accattggta agagtctgtt tgaatttcga tcaaatttgg taattccaac
    27841 acttcactga ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt tactacgtgt
    27901 ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa gactacccga tcaattgtta
    27961 caataatatt tcaaatatta tttttaggta acaacagaca ttttttgcgg cttttaggca
    28021 aaaaaaaacc aaaaatagaa cattctcttg aaaccttact tcgtgagctt ctacttttgt
    28081 ttccttatat taaagccgaa acggacaata tttcgtttct gccttttctt catcagatag
    28141 tttttgcaac ttcatatact acacttattt tctataaaag tcaacacttc ctggcgtttc
    28201 ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa ttatctgaca
    28261 attcttaatt gacagacctt tttatctcgg ttatacttta gaaaattcat tttgtggagg
    28321 aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca caactgaata acttttactt
    28381 tagctacttt agataggttt gtattcatca tgatggatac aaacgttaaa cgacagtttg
    28441 tatctatgat ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac aaaatgctaa
    28501 gtggctgagc agtgaaaata caaagtaaaa gtatcaagca ttctactttt tcaatttatt
    28561 ttccgacttt tcaatccatt tggacatttt gtctgaatgg attttttgtt tcccaaaatt
    28621 aaggaggaaa aagaaatgaa aaaaaatata atcatcggat taggcgctgc cctgctacta
    28681 gctttaggag gttgcgcaca aaaagacaat tccgcagagg catcagatgg acaaaaaaca
    28741 attggcgtat tgcaacttgt acaacataac tcgttagatg ctgcctataa aggattcaaa
    28801 gaaggattag cagaagaagg gtataaagaa ggcgataatc taacaatcga ttatcaaaat
    28861 gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa agaagatcct
    28921 gatttgctac ttggaatcgc cactccagca gcacaatctt tattaaatga aacaaccact
    28981 atcccgatga ctgtcacagc ggtcacagac ttggaacaag cccatttagt cacatccaat
    29041 gaaaaacctg gcggcaatgt gactggcacg acagatatgg tgccaatcga aaaacaaatc
    29101 ggcttactgt taaatatcgt gccagacgcc aaaacaatcg gcatcatgta taacaacggt
    29161 gaagcaaact ctaagatcca agctgatttg gcagaaaaag aattgaaaaa agcgggcgtc
    29221 aaagtcaaag tactgacagc aaattccaca aatgatgtcc aacaagtgac gaaaagtcta
    29281 gctaaagatg tagatggaat ctatattcct actgataaca cgttcgcaag tgcggcaagt
    29341 gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc agttgatcaa
    29401 gtcaaagacg gcggtctagc aacagtagga atcgactacg aaagcttggg caaacaaaca
    29461 gggatcatgg cagcaaaaat cttagacggc aagaatccaa gtgaacttcc tgtcgaaaca
    29521 gcgaatgatt tggaactgta tgtcaataaa gacatggcca aagcattagg catcaatcca
    29581 gacagcatca aagaagctga ataaaacaca ggaggattct catgacaaat ttgctctctc
    29641 ttttcttatc atctgcatcg caaggggtct tatgggccct acttgcgatt ggtgtatacg
    29701 tcactttccg tatcttggac attgccgact tgactgctga aggaagcttt cctttaggtg
    29761 cagggatcgc ggcagtaagt attacaaatg gctatcatcc attgacagct tgtcttctag
    29821 gctttttagg gggtgcggca gcagggcttg tttccggact gcttcacaca aaattgaaaa
    29881 ttcccgccct tcttgcaggg atcatcacca tgactggttt atattctgtc acatcccgtg
    29941 tgatgggtgc gccgaacatc tctttattgg gtcagccaac gatctttcat tgggtcgagt
    30001 cattcggcat aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt gctttgatcg
    30061 ttgtatgttt acttgttttg ttcttacgta cagaaacagg acttgctatt cgtgcaactg
    30121 gcgataatct ggcaatgagc gaagcaaacg ggatcaacac agataatatg aagatcatcg
    30181 gttatatgat ttctaatgga tgcattgctc tttctggctc cttacttgcg caaaacaacg
    30241 gctttgctga tttaaattct ggtattggaa cgatcgtaat tggacttgct tctattatca
    30301 tcgcagaagt tttgttacgt aaccagccac tatttctccg tctcttgact gtcgttttag
    30361 gtgcggtcat ttatcgtttt attttagcgt tagtcttcga actgaatgtt gaaccaagtg
    30421 attcgaaact tgcttctgct ttcgtgctag tgatctgtct ttctttcccg cagatccagc
    30481 aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg acgtcttaac
    30541 attggaaaat ctccatcata cctttgaaaa aggaacgatc aacgaaaatc atgtcctgaa
    30601 aggcattaat ctttccttag atcccggcga cttcgtgacg atcatcggtg gaaatggggc
    30661 tggtaaatct actttgctga acagcatcgc cggtacattt tctgtcgatc agggtcaaat
    30721 tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga aatccatcag
    30781 tcgcgtattc caagatccta aactagggac agccgttcgt ttaacggtag aagaaaatct
    30841 tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca aaccacaaga
    30901 ccgaacattt ttcaaagacc agctggttcg gctaaatttg gggttggaaa atcgtttatc
    30961 ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct attacgttat tgatggccac
    31021 attacagcgt ccagaactga tcttattaga tgaacacact gcagcgcttg accctgctac
    31081 ttccaaaacg gtgatggaat tgacagaaaa gctgatcact gagcagaatc tcactgcttt
    31141 tatggtcact catgacatgg aagacgcgat ccgctatggg agtcgactga tcatgctgca
    31201 tcaaggaagg atcgtcgtag atatccagca agaggaaaaa cagcagctca cagtgcccca
    31261 attattagcg ttgttcaaac aaaacagcgg cacggcatta aaagacgatc aagtactttt
    31321 atcttcataa tcagaaagag gatgggacaa aaaatgaacg cccaccctct tcttttttct
    31381 ctttccttct ttcgtttttg ctttatgtat caaccgttgt ttcttcgaaa actattttgt
    31441 tctctccttc ttcctgatag aatggatggg aaaggagctt gatgccatga caacgaaaga
    31501 caaggtactt caattattga aaaagacacc agattttctt tccggagaaa agttggcaca
    31561 gcaactggaa gtttcccgga caagtatttg gaaagccatc aaagaattgg aaaaggcagg
    31621 ctatcgtttt gagcacgggc ctacagggta tcgttacctg ccctctgacg tactagatgc
    31681 aaaagaaatc cagcaagagc tacggtcgtt tattcctgat ttgaaagtac aaacgatgtt
    31741 gacttctgaa tctacgatga aagatgccaa actggcagca gctcaaggag attctgtttc
    31801 gaagttattt gttgctgata cacaagaaca agctcgtgga cgttttggac ggcctttttt
    31861 cgctgaacct ggacgaggaa tctacatgag tctcttgctc catccagaca aacggtttga
    31921 tgaattgcct caatatacga tcatcgcagc cagtgcctgt gtgaaagcca ttgaatctgt
    31981 aaccggtcgt tctgcttcga tcaaatgggt caatgatatc tacatggatg gaaaaaaagt
    32041 ctgtgggatt ttatctgaag cagtcagtga tatggaaagc ggcaggatca gtcatatcat
    32101 cattgggatc gggcttaatt tttctgttcc tcaaaatcat tttccagctg atctccaaga
    32161 aaaagccatc tctgtctttc ccgacggaca agctagtgtc acaaggaatc agctaatcag
    32221 caaaatctgg caagaattgt tcacttatct ttctcaccca gaaaaagcgt tggacttcta
    32281 taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc aatcaaaaag gaacgactta
    32341 tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg atcgtccaaa caaatgaaca
    32401 gaagatgagt ttgtcttctg gcgaaatcag tctcacatct atccgtacgc aataattatc
    32461 agatttcaca aaaggacacc tgatctcata cagtgtcctt ttttctgaat tctcttcaaa
    32521 agactctcct tattctatca gctgacagtt tctctatcct ctagaaaaag gtattctaaa
    32581 aatatctata aagcatggta cactttcttt aaatgaatca atgcaaaaaa caagagagtc
    32641 atcagtttac acgatcaaaa gagatagcag gccgcttcct ttttcaatct tatagataga
    32701 ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta ttttgccgta agttcttttt
    32761 tagctagtgc cttaatctct taatcccaac aaacgtctcg catagatcac ctgaccagag
    32821 tgcatagcag catcatcaat aatagaaacg agacgcattc ctcgtgtgac tggtggtgtc
    32881 cagttctcgt caattacttc atctagctcg gaaggttcca gccttcttaa gtaagaaata
    32941 gcaaaatcag cagctcctcg caagtatcct agcagatctt ctatttgatt agcttggatc
    33001 tgctttgctt gttccagact atgcttccaa tcttgttcgt tatctgctat cgtcatcgga
    33061 aaccggcttt cccagtcttg actatgccaa attggagaag ttcctgctaa atcagcaatc
    33121 tggaaatcta attctcgagc ggtatgccaa gccagccaag tcattgattt gatctgcggg
    33181 gctctatctt ccactggaaa acgattggcc tcctcaatag agacgccttc aaacaaacgt
    33241 tcaaaccgat cgtatgcccg atccacactc tcgcatagca tatctaaata atccatttat
    33301 tttcctcctt tttatttatc ctacttactt tttcctaatt ctaggatacc tacttatatg
    33361 ttactacaca cccttatttt ttacacttta acagtcacca tcatcaatag tttggagatt
    33421 gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa gcaacaatat gaacgtttca
    33481 ttaatattct ttaaataaac aatgattttt caaacgaaca gtttaagact aggagaaaaa
    33541 gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa aaatgaaaaa aagggcaagt
    33601 ttactagtcc tagttgcttt gttgttcaat atcttttcgt atggtgtggc cgtttcagcg
    33661 gaaacataca ttcctaaaga ccctgggaat atttataatg atctaactgg taactatggc
    33721 ttgttaggaa ttgctagtca attccacgta tttgctaaaa ataagacaac tgtgaatgca
    33781 catacagacg gaaatatagc aacacatgaa cttgatgcaa aaaataattt tggtacagag
    33841 cttcatgaag gtttattgaa ccaagaaata aattatatac aaaaaactga ttctttcgct
    33901 aattcttcag gcatacctac aggtagcaca agaatgaata aatttgtcgt tggcagtaac
    33961 gtggatgtag cacttcaaga tggtcatcct acagtcaatg gatccaggct tgatcactta
    34021 actccagatg atttttataa agatcgtgct gggcatgaat atattgattt cgagcaggaa
    34081 tttgctaaat tgaatgtagc agcaaatgat cttgcaacga tcactccagc taaaacatat
    34141 actgctgcag attttccaga catgaacaat cggacaatcg atttaactga tttatcagat
    34201 accgggtttt tattggtaaa tatcgatgca gaagtgttaa cgatgaatac gccacttcaa
    34261 atcattaatc caaatgatca agtagttgta tttaatgtca tcaacagttc ttctgcattg
    34321 aacgtacagt caccaatcaa atacaatgat cgttcaaatc atgaaacaga agatttttcg
    34381 gatgcgaata tctcttggaa ttttggaaat gagatgactg atttgacaat cagtgctcct
    34441 tttcaaggaa caatccttgc gcctaatgct actatccgca ttactcaaaa ccaagacgga
    34501 acgattatag gaacaaatgt gattttagat gcagctacca atcgttggga tcctaacgaa
    34561 atatttatca gcgacaatgg tacagatacg acagacacaa gcgacagtac agatacatct
    34621 accacaagtg atagctcaga ttcatcaact tcaagcgaca gtacagatac tacctctacc
    34681 acaagtga
//
LOCUS       DANKAZ010000020        34330 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000020 DANKAZ010000000
VERSION     DANKAZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            202..1077
                     /locus_tag="PWY67_000922"
     CDS             202..1077
                     /locus_tag="PWY67_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057227.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="PWY67_000923"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="PWY67_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="PWY67_000924"
     CDS             complement(1642..2973)
                     /locus_tag="PWY67_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2057228.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="PWY67_000925"
     CDS             complement(3122..3835)
                     /locus_tag="PWY67_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2057229.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="PWY67_000926"
     CDS             4010..5434
                     /locus_tag="PWY67_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2057230.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="PWY67_000927"
     CDS             5733..6440
                     /locus_tag="PWY67_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2057231.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="PWY67_000928"
     CDS             6427..6750
                     /locus_tag="PWY67_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2057232.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="PWY67_000929"
     CDS             complement(6839..7876)
                     /locus_tag="PWY67_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2057233.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="PWY67_000930"
     CDS             complement(8197..9180)
                     /locus_tag="PWY67_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL2057234.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="PWY67_000931"
     CDS             complement(9595..10365)
                     /locus_tag="PWY67_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057235.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="PWY67_000932"
     CDS             complement(10358..11293)
                     /locus_tag="PWY67_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2057236.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="PWY67_000933"
     CDS             complement(11609..13258)
                     /locus_tag="PWY67_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2057237.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="PWY67_000934"
     CDS             complement(13375..15978)
                     /locus_tag="PWY67_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2057238.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="PWY67_000935"
     CDS             complement(15990..17039)
                     /locus_tag="PWY67_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDL2057239.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="PWY67_000936"
     CDS             complement(17065..17379)
                     /locus_tag="PWY67_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDL2057240.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="PWY67_000937"
     CDS             complement(17395..17841)
                     /locus_tag="PWY67_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2057241.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="PWY67_000938"
     CDS             complement(17842..19764)
                     /locus_tag="PWY67_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL2057242.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="PWY67_000939"
     CDS             complement(19976..20872)
                     /locus_tag="PWY67_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2057243.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="PWY67_000940"
     CDS             complement(20865..21545)
                     /locus_tag="PWY67_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2057244.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="PWY67_000941"
     CDS             complement(21546..22328)
                     /locus_tag="PWY67_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057245.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="PWY67_000942"
     CDS             complement(22321..23238)
                     /locus_tag="PWY67_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2057246.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="PWY67_000943"
     CDS             complement(23402..23725)
                     /locus_tag="PWY67_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057247.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="PWY67_000944"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="PWY67_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2057248.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="PWY67_000945"
     CDS             27853..28167
                     /locus_tag="PWY67_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2057249.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="PWY67_000946"
     CDS             28180..28554
                     /locus_tag="PWY67_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2057250.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="PWY67_000947"
     CDS             28564..28899
                     /locus_tag="PWY67_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL2057251.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="PWY67_000948"
     CDS             28982..30322
                     /locus_tag="PWY67_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2057252.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="PWY67_000949"
     CDS             30400..31074
                     /locus_tag="PWY67_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2057253.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="PWY67_000950"
     CDS             31067..31231
                     /locus_tag="PWY67_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057254.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            31307..32449
                     /gene="dcm"
                     /locus_tag="PWY67_000951"
                     /pseudo
     CDS             31307..32449
                     /gene="dcm"
                     /locus_tag="PWY67_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            32439..32729
                     /locus_tag="PWY67_000952"
     CDS             32439..32729
                     /locus_tag="PWY67_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057255.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /locus_tag="PWY67_000953"
     CDS             32988..34172
                     /locus_tag="PWY67_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2057256.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34169..34306
                     /locus_tag="PWY67_000954"
     CDS             34169..34306
                     /locus_tag="PWY67_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2057257.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT        10701 a         6609 c         6254 g        10766 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DANKAZ010000021        33727 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000021 DANKAZ010000000
VERSION     DANKAZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(113..1081)
                     /locus_tag="PWY67_000955"
     CDS             complement(113..1081)
                     /locus_tag="PWY67_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL2057258.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1313..2146
                     /locus_tag="PWY67_000956"
     CDS             1313..2146
                     /locus_tag="PWY67_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL2057259.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2180..3019
                     /locus_tag="PWY67_000957"
     CDS             2180..3019
                     /locus_tag="PWY67_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2057260.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3045..3605
                     /locus_tag="PWY67_000958"
     CDS             3045..3605
                     /locus_tag="PWY67_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2057261.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3804..5525
                     /locus_tag="PWY67_000959"
     CDS             3804..5525
                     /locus_tag="PWY67_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL2057262.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5690..6832
                     /locus_tag="PWY67_000960"
     CDS             5690..6832
                     /locus_tag="PWY67_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2057263.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6848..8059
                     /gene="thiI"
                     /locus_tag="PWY67_000961"
     CDS             6848..8059
                     /gene="thiI"
                     /locus_tag="PWY67_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL2057264.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            8632..9279
                     /locus_tag="PWY67_000962"
     CDS             8632..9279
                     /locus_tag="PWY67_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2057265.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    9408..9624
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9672..12317
                     /locus_tag="PWY67_000963"
     CDS             9672..12317
                     /locus_tag="PWY67_000963"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL2057266.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            12627..13940
                     /locus_tag="PWY67_000964"
     CDS             12627..13940
                     /locus_tag="PWY67_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL2057267.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            13930..14583
                     /locus_tag="PWY67_000965"
     CDS             13930..14583
                     /locus_tag="PWY67_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2057268.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            14638..15189
                     /gene="radC"
                     /locus_tag="PWY67_000966"
                     /pseudo
     CDS             14638..15189
                     /gene="radC"
                     /locus_tag="PWY67_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            15320..16405
                     /locus_tag="PWY67_000967"
     CDS             15320..16405
                     /locus_tag="PWY67_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2057269.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            16402..18294
                     /locus_tag="PWY67_000968"
     CDS             16402..18294
                     /locus_tag="PWY67_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2057270.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            18301..18678
                     /locus_tag="PWY67_000969"
     CDS             18301..18678
                     /locus_tag="PWY67_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2057271.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            18832..19614
                     /locus_tag="PWY67_000970"
     CDS             18832..19614
                     /locus_tag="PWY67_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDL2057272.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(19740..20471)
                     /gene="erm(A)"
                     /locus_tag="PWY67_000971"
     CDS             complement(19740..20471)
                     /gene="erm(A)"
                     /locus_tag="PWY67_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDL2057273.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(20529..20588)
                     /locus_tag="PWY67_000972"
     CDS             complement(20529..20588)
                     /locus_tag="PWY67_000972"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HDL2057274.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            20980..21642
                     /locus_tag="PWY67_000973"
     CDS             20980..21642
                     /locus_tag="PWY67_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2057275.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            21877..22020
                     /locus_tag="PWY67_000974"
                     /pseudo
     CDS             21877..22020
                     /locus_tag="PWY67_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            22170..22370
                     /locus_tag="PWY67_000975"
     CDS             22170..22370
                     /locus_tag="PWY67_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2057276.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(22624..24303)
                     /locus_tag="PWY67_000976"
     CDS             complement(22624..24303)
                     /locus_tag="PWY67_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2057277.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(24305..24517)
                     /locus_tag="PWY67_000977"
     CDS             complement(24305..24517)
                     /locus_tag="PWY67_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL2057278.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            24910..26640
                     /gene="efrA"
                     /locus_tag="PWY67_000978"
     CDS             24910..26640
                     /gene="efrA"
                     /locus_tag="PWY67_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL2057279.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            26637..28397
                     /gene="efrB"
                     /locus_tag="PWY67_000979"
     CDS             26637..28397
                     /gene="efrB"
                     /locus_tag="PWY67_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL2057280.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            28492..28689
                     /locus_tag="PWY67_000980"
     CDS             28492..28689
                     /locus_tag="PWY67_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057281.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            28842..29327
                     /locus_tag="PWY67_000981"
     CDS             28842..29327
                     /locus_tag="PWY67_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL2057282.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(29431..30024)
                     /gene="clpP"
                     /locus_tag="PWY67_000982"
     CDS             complement(29431..30024)
                     /gene="clpP"
                     /locus_tag="PWY67_000982"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL2057283.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            30204..30731
                     /locus_tag="PWY67_000983"
     CDS             30204..30731
                     /locus_tag="PWY67_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057284.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            30822..31559
                     /locus_tag="PWY67_000984"
     CDS             30822..31559
                     /locus_tag="PWY67_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2057285.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            31563..32480
                     /gene="miaA"
                     /locus_tag="PWY67_000985"
     CDS             31563..32480
                     /gene="miaA"
                     /locus_tag="PWY67_000985"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL2057286.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            32482..33711
                     /gene="hflX"
                     /locus_tag="PWY67_000986"
     CDS             32482..33711
                     /gene="hflX"
                     /locus_tag="PWY67_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL2057287.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        11317 a         5582 c         7061 g         9767 t
ORIGIN      
        1 agaaatttac gaaaattgtt tctcaaattt ttgtaaattt tcgacttagt tttcgaagaa
       61 gctatttttg tcacagcctc tctttttaag tttagctatt agataatgat gtttagtttt
      121 ggataaacga aatcaattct tcacccgaca aaccatagtt tacagcaatc aatagtttta
      181 aacgtgcttt ttgactatta atactgttac agaaaataat ccccattttt tctagttctt
      241 ttcccccgcc ttcataatca taaacaggct ctgcgattcc attgaaacaa cgagaaacaa
      301 gtactacagg gattttttgg acaagaagct tttccaaagc agctaaggct tgtggaggga
      361 gattccccgc acctaaagct tcgattacca aaccgtctac tttcgtatgg gcgatggctt
      421 ccaataagtc cccttgcatc ccagcatatg ctttaacgat cgggatagtc ccatctacag
      481 cggaaatgtc cagtcgcttt gtttctaaaa gttcctgcaa gaataaaatt cgattcttag
      541 ttactaagcc gatgggaccc aatgtaggtg ttcggaaagt agcaacattt gtggtatggg
      601 tctttgtcac gtagcgtgca gaatggatct catcgttcat gaccaccaaa acccccttat
      661 ctagcgcttc ttcacaactt gcgacacgaa tcgcactttc aaaattataa aggccgtcac
      721 ttccaagttc attacttgaa cgcattgccc cagtcaaaac aattggtaat ttgttgccaa
      781 tcgttgtatc aagaaaaaaa gcagtttctt ctaatgtatc tgtaccgtgt gtgatcacca
      841 ctccggatat acctgatgca taagcggaca agatacgttc ttttaatacc aacatgtgag
      901 aaggcatgat gtgtggtgag ggaagattga agatatcttc aactaataat tctacatgct
      961 tggggattgt gatttctgca tctaataatg ggtttgccac atcaggacta acaccgcccg
     1021 tgagcatgtt ttctttcatg gcgatcgttc cgccggtgtg tagtacaagt atttttttca
     1081 ttttacagac ttccttacga ataattttgc atagccgtta aatcttattg tatactatat
     1141 aaaagagttt tgctatcagt gaagaataac gaatagccta acttatcata aaaaagctga
     1201 ccttattaaa atggaaaaga ctcaaccttg acctcctaat acaatggagt atctggttga
     1261 gtttctttta caagaaagca gccaaggaaa tcaatcacga aggtggccgg aaatgatcag
     1321 aatcggatta acatcgttta aagaacatga caaattaact ggtaaaaaga ataacactct
     1381 ttatgaatat gcagggtttc tacccttagt ggaattagat actgcctatt atggaattcc
     1441 ccgaaagtcc tcagttgaga attggataag tgaagtacct gaatcatttc gctttgtagt
     1501 caaagtctat caaggaatca gcggtcaagg agactggaag ccttattatc agaccgaaga
     1561 agagatgatt gcgcattttt tagatgttat gtcgccaatg gtagatgctg gaaagatgtt
     1621 ttgcttttta gtccaattcc cctcatcatt caaatgtacg aaagaaaatg tccagtactt
     1681 gcgcaaactt agaaagtggt ttgagggtta tcctgtttct atagaattgc gggattattc
     1741 ttggtacagc gatgaatttc ttgaacgtac aagaaacttt atgtctagag ccgctttttc
     1801 tttagccatc atcgatgagc cacagttgct gaatacaacg attccttttg atccatttgt
     1861 caccaatccg aactttgctc tttttcgatt ccatggtagg aatgcgcaag gatggcaagc
     1921 aaatgataaa gagtggcgta aaaaaagaac gttatatcgt tattcagaaa aagaactgca
     1981 agaactagca catgaagtca aagtagttga ttctaagaca aaagaggtag gtgtgatttt
     2041 taataataat tctggtggtg atgcggctga aaatgcatta gcactgcaaa aaatattaaa
     2101 tatcaagcca gatggactca atccaaccca aatggatctg ttttaagggc ttattttatt
     2161 caagaaaggg gcgaagtcta tgatcaaagc tatttttttt gatattgacg gaacattggt
     2221 caccaatcgt tcaaaagcgt tggaatcaac aaaacaagca attgaatatg ctcggaaaaa
     2281 cggcatttta tgcggtgtag caacggggcg cagcccagta aagatcaaag aaatcattga
     2341 tgaattggaa ttagatatgt atgtcgtgta taatggtcaa ctggttttta ctgcagatcg
     2401 cacgattatc gaccaccctt ttgaacaaaa agtattggaa catattgttg aatttgcaga
     2461 tgagaaccat cgacaaatcg tttttggtgc gagaaatcgg ttagatggat caacgacgat
     2521 gctattagga caatcgattt ttatcaagcg actagtcagc tttttaccgc gtaaattccc
     2581 ggtacgtctg atgaaaaaaa tcctccaggt ttttagccct caccgacaaa aggatcgtta
     2641 tgaaacgtta gagatattaa aagaaccaat ttatcaatgt atattgctca gtccggagtc
     2701 agaacagaaa aagttagaac atcgctttcc cgagtgtacg tttcagcgtt caaatacgta
     2761 tactgtagat atcatcccaa aaggtggttc aaaattacta ggcatccaag ctttcgcaaa
     2821 tgctgtcgaa attgaaatgg aagaaattat ggcattcggc gatcattaca atgatatcga
     2881 gatgctgaaa ggtgtaggaa ttggagtggc catggggaat gctcagatcg aagtaaaaca
     2941 tgctgcagac tttgtgactc aatcaaatga agaagatggg atatacgaag gactgaaaca
     3001 cttcggtctg atttattagt caatgaaaga aggaaaaaat cagtatggaa gcagagacac
     3061 cttttcaaaa agtagaaaca ttccacaaaa cttttgatcc aagaagacca gagataccca
     3121 cggcttttac agaaaatgaa gctcatacac gtgctgcgtt caaaatagaa gaacttgttg
     3181 aatttgttgc agctacttca cctaataaag aacaactgca tgaagctgtt atgctgctgc
     3241 acgaagcatt agataaagca gaagaaaaaa tacttggtaa aacacagtgg ggaagtgatc
     3301 cgctggttga acaagtagat gcactgacag atatccttta ctttacttat ggttcttttt
     3361 cattactagg aatagtgcct gatcgaatat tcgaaatcgt ccatcaggca aatatgggga
     3421 aattatttcc agatggaaaa cctcattatg accctgtaac aaataaagtg ttgaaacctg
     3481 aaaactggca gatggaatat gctccagaac ctaaaatcgc ggcagaaatc caacgtcaga
     3541 ttcaagaagc gattgctaag aagaaatccg ctgatatagg catgtgcaca aatgaaaaag
     3601 agtgatcaga gtttcactat ttttgttatc tttatacgat ggaaaaacaa aagattttcg
     3661 tttaagttag tgaatgcatg aaaatagtat gctataatct agaatagatg tcttttgcac
     3721 gagagacgta ttatttggat ttatgccaag cagaacgcca tctgttgcat aaaagatgca
     3781 tactgaaaac ggaggaacca taaatgggaa ataatgttat catcggaatc gtcattgcaa
     3841 tcattatagt cgccattgtc gtctttttgg ttagttactt gatgaagaaa aaaaaccaag
     3901 atcgattgga tgccttagaa aaacgcaagg aagaattatt tgacttgcca gtgattgaag
     3961 aagtcgatga tgttaaaaaa atgcatctgg ttgggcaaag ccaaaatacc tttcgtgaat
     4021 ggagtcaaaa atggaatgac atatctactt cctcgttcgc tgaactagaa agtcaaattt
     4081 tcgaagtgga aaacctcaat gaaagttttc ggttcttaaa aggaaaaaaa gcagttgttg
     4141 aagcagaagc tacaatggac caaatggaga aacaagtgac ggaaatccgt gaaggtctaa
     4201 aggaattgcg ggaaacggaa gaacgcaact cactagctgt tcagcaggca cttgacgtct
     4261 atgaagaact gaaagaaaaa gtcagagaag acgagcatga atacggacca gctctttttg
     4321 aattgcaaaa acagatcaaa gtgatcgaaa aagaatttac gcagtttgtg actttaaaca
     4381 cttctggaga ccctgttgaa gcccgcgagg ttttggataa agcagaaaaa catacgtatg
     4441 aagtggaagc tctgatgaaa aaaattccac cgctgtatga ggagctccat accactttcc
     4501 ctgaacaact tgaagaaatc agtgatactt acgagaaatt gatggaagaa caatatgtat
     4561 tcccagaaga agatcttgct gaagatatcg caaaagtaag tcgaagaatt gaaaattcat
     4621 tagccaatct tgaaaaaaca gaagttgaaa cggtcgaatt tgaaaaccgt gaaacagctg
     4681 atttgattga ttcgttatat gatatcttag aacgggaaat ggaagctcag agatatgtaa
     4741 aaactaacca atcaacgatt gcagagtata tcaaacatac aacaaagaat aaccgtcaat
     4801 tattgattga gctggatcac acatctcaaa gctatacatt gaaccacaat gaaattggcc
     4861 gtgtacgagg tttccaaacc gaagtggaag aaatggaacg tcaaaatgaa cagatgattc
     4921 cacagatccg tcaacacgaa attccttatt cagaagtgcg cacgttctat aaaacagtct
     4981 tcaaagtctt agaagatatc gaaaagcaac aagtagagat cgacgactca ttacatgaat
     5041 tgagaaaagg cgaaaaagaa gcgcaagaaa agatcgatac attcgaattc aaattgcgca
     5101 gtttgaaacg ttttgtagaa aaacaacgtt tgcctggctt gccgaatgat tacctagaat
     5161 tcttttttgt tgctactgac cgtattgaag aactaagtgt tgtcttgaat aaaattcgag
     5221 taaacatgga agaggtcaat cgtttagttg ctctatgtga agaagatcta gaattattag
     5281 ataagaagac acatgattta gtagatgcag cagcattaac ggagcaaatg ctccaatatg
     5341 caaatcgtta tcgtcataca cacgaagaag tgagagaagc aattgataaa agctactatt
     5401 tattcaataa agaatatcac tatcaagaag cattagacga aatcggtaca gcattagagc
     5461 gagtagaacc tggtgctttc aaacgcatcg aagattttta ttttaatcat ccagacttag
     5521 tataaagcaa aaaagttagt aaaccagcaa gagcaagacg gattattgtc ttgttcttgt
     5581 ctttttttgt atatttgaaa attgaaacaa gtgaaaattt cacctgctct tgaaagcacc
     5641 ttggacaacc gttataatag aaaagaattt caaaaaagag gggtttctta tgatatattt
     5701 tgataacagt gcaacaaccc caatctttcc gcaaagctta gatgcatatg taaaaaccag
     5761 tcagcggatt attgggaatc catcaagctt gcatgattta ggcaatcaag ctaaccggtt
     5821 gttgcagcaa gcaagaaagc agatcgctga tttgatccat gtagaaccag aagaaatcta
     5881 ttttacttca ggcggaacag aaggagataa ctgggtatta aaaggaacga tgatcgaaaa
     5941 aagagaatat gggaatcata tgatcatttc aggagtcgaa catccggccg tcagcgagac
     6001 agcagaacaa ttaaaagaat taggaatcga agtaagtatc gccccagtgg ataaacgcgg
     6061 atttgtacgt gtcgacgaat tgaaagaatt aataagaaaa gagacagtat tagtctctgt
     6121 tatggctgtg aataatgaag taggatcgat tcagcccatc caggaaatca gtgatctttt
     6181 ggatgatttt cctaaaattc atttccatgt agatgctgtt caggcgattg gaaaagtacc
     6241 attctcgcaa tggttgacag aaagagttga ttttgcaaca ttttcagctc ataaattcca
     6301 tggcccacgg ggaactggtt tcatttattg gaaaaagggt cgtcgtttag caccattact
     6361 gacaggtgga gggcaagaaa gaaatcagcg aagtgggaca gaaaatgttc caggtattgt
     6421 ggctatggtt aaagctttac gtttatcatt agagataaag gaacaaaaac ctgagcagac
     6481 acaacgctta aaagtagctc taatcaatgc gttgaagacg tatgagaaag tgacgatttt
     6541 ttcagaagga actgactatg caccgcatat tttatgtttt gctctaaaag gaattcgcgg
     6601 cgaagttctc gtccatgcct tagaagaaaa acaaatattt gtttctacta ctagtgcctg
     6661 ctcctcacgt aaacatatgg caagcagtac attgcacgca atgcatgtac caaatgatct
     6721 ggctacatct gctgttcgaa tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca
     6781 atttatgatt attttcaagc agctttataa gaaattttct gcaatcaatt aaaagaggag
     6841 tgttcttgtg aaatacagtg aaattatggt ccgttatggg gaactttcga caaaaggaaa
     6901 gaatcgaaaa agctttatca tgcaactagc acaaaatgtc agacaagctt tgaaagactt
     6961 tccagaaatc aaaatccatg ctgatcggga ccggatgcat cttttattga atggcgcaga
     7021 cgggcagcta gttattccaa aactagcaaa aatatttggg atccaaaatt tctctccaag
     7081 tattcgtgta gaaaaagatg tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga
     7141 aatttatact gggaaagaaa catttaaaat tatcgctaaa agaagcgatc accaatttga
     7201 attgacaagt aatgaattga atcaaacatt aggtaatgct gtttttgata ttttcccgca
     7261 tatccaagtt caaatgcgtc aaccagatat tccattacgt gtagagattc ggcgtgatgg
     7321 cgcttatcta tcgtatgaaa cgatcaaggg agctggagga ttgcccgtag gaacaagcgg
     7381 aaaaggaatg cttatgcttt ctggggggat tgattctcct gtagcagggt actttgccat
     7441 gaagcgggga gtggaaattg aagctgttca ttttgctagt ccgccgtata caagtgaaca
     7501 agcactgcaa aaagcaaaag atctaacagc caaactagca ccttatgtgg gaacgatcca
     7561 ttttatcgaa gtgccattta ccgagattca agaagaaatc aaaaagtcag tgccacaagg
     7621 atattggatg acaattactc gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat
     7681 tcgtcatggt ttagtcattt tgaatggtga atcattaggt caagtggctt cacaaacttt
     7741 gcaaagtatg gtggctatca atgaagtaac gaatacgcca atcattcggc ctgtagcgac
     7801 aatggataaa cttgaaatca ttgaagtagc agaacagatc gatacattta atctttctat
     7861 ccagcctttt gaagattgct gtacgatttt tgctcctcca cagccaaaaa cacgccctca
     7921 actggaaaag gtccatcaat atgaagagcg tttagcaata gatgaaatga tccaacgagc
     7981 actagctggc ttgaagatgg aagagataca agcagaatcg tctaatgaaa aaactgaaga
     8041 attttcagaa ttgctataag aggttatgaa aatagtgttt tgaccagagt attagagaaa
     8101 caaaacgaaa aaatagctcc atgtatttta ccgtttttga actaatgtcg aagattagct
     8161 gattgaatac catttatcaa gaagttatgg aaaagccggt tcttttttac taactgaaaa
     8221 ataaaatttt atttagagat caagctacag ttgataaatg atataaaaaa gagaatttcc
     8281 catgaaatag cgaggttgtg acagaagtgg gcagcttcaa gaaataagaa ggaatttacg
     8341 aaaattgctt ttcaaatttt tgtgaaattt cggcttattt ttcgaagaag ttacttctgg
     8401 aacaccgtgt atcagaggtt gcgaaaaagc tgccctgcat caagtaataa gaacagccaa
     8461 acaaaaatag ttcctcatat tttacgtttg gttgagctta ttaccgcaga tgcaagcttt
     8521 tgaacaccgt tttatttgtc tcacaccctt ttccttccta cttgtaacct tgctcccgtg
     8581 ttagaaacat gttacactaa atatgtgaaa ttattaacaa ggagcgtcgt tatgcgagaa
     8641 aaaacagata cactgccaaa agcaactgcg aaaagattac ctttatattt acgctacttg
     8701 aagatgctgg atgactcagg tataagtaga atcaaatcaa atgagttcag cgaaatcacc
     8761 caaattcctt ctgcaacgat ccgcagagac ttctcgcaat taggcgagct aggcagaagc
     8821 ggatatggct atgatgtccc ttttcttatc gatgtattca ataatatcct taatactaaa
     8881 gaagaaaaac gaatcgcatt agtaggatat ggaaatcttg gcaaagcatt gaaacacaac
     8941 aattttagaa gaaatgaaaa tctaaatatc gtgtgcgtct ttgataatga tccagcactg
     9001 atcaatcgag taattgatgg agaaatgatc tatccgattg atcgtttcgc ggagatagcc
     9061 aaagcaaaaa atgtgacagt agcaatctct acggtaccaa gtaaatattc acaatctgct
     9121 attgatgaaa ttgtcaaagg aaatgtaacc gctatactta attttgcgcc tgacagagta
     9181 actgtcccag cttatgtcaa tgttcaatac attgatttga caaccgagtt gcagacgctt
     9241 atttactttg atgagaatta ctcagaagtt ttttcttagg aaaatttgag taggcttatt
     9301 gatcttgcga gaactcgtcg taattctttc aaaaattttt tagtactaaa tgaagtggtt
     9361 ttttcacgac cacttgttga ctaaaaaaaa aaaccattgt agaatgaagc ataacgctga
     9421 acaagaggag taactggcac attcattctt agagagagaa ctatctgctg aaaggttctc
     9481 aatgacaacc agtgaaggta gcttgggagt ttctgtcttg aatggagtag agatagacga
     9541 gtcgtgatcg ttaactcact tgagaggcaa taatgattga ttattgcaat ttaggtggta
     9601 ccgcgtaaat catacgtcct agaatacgaa agtattttag gacgtttttt agtttaaagg
     9661 aggaaaacag catgacggaa gaaaataacc tatcaacaaa atataatccg caagaagttg
     9721 aagctggacg ttatcagcat tggttagaac aagatttgtt taaaccgagc ggagataaaa
     9781 aagcaaaacc ttattcaatc gtaatcccgc ccccaaatgt tactggaaaa cttcatttag
     9841 ggcatgcttg ggatacgacc ttgcaagata tgctgattcg tcaaaagaga atgcaaggat
     9901 atgatacttt atggctgcct ggtatggacc atgcaggaat cgcgacacaa gcaaaagtag
     9961 aagcgaagct tagagagcaa gggatttctc gttatgatct aggtcgtgaa aagttcatcg
    10021 atcaagtgtg ggaatggaaa gaagagtatg cgagccacat ccgcgaacag tgggctaaaa
    10081 tgggcttatc tttagattac agtagggaac gttttacatt agatgatgga ttatctgaag
    10141 cagtacgtaa agtttttgtt accttatacg aaaaaggttt gatttatcgt ggtgaatata
    10201 tcatcaactg ggatcctcaa gcacgaacag ctctatctga tatcgaagtt attcataaag
    10261 atatcgaagg cgccttttat catatgagct atgaactagc agatggatca ggcgtagtag
    10321 aaattgcgac aacccgtcca gaaacgatgt tgggagatac ggcgattgct gttcatccag
    10381 aagacgagcg ttatcaagcg ctcatcggca aaaaagtgat tctaccttta gtcaataaac
    10441 aaatcccaat cattgcagat gaatatgtag atatggaatt cgggacaggt gtagtaaaaa
    10501 tcactccagc acatgatcca aacgactttg aggtaggtaa tcgtcatgat cttccacgcg
    10561 taaatgttat ggatgatgat ggtacgatga atgaactagc aggaaaatat gctggaatgg
    10621 aacgttttgc tgctcgtaaa gcaatcgtgc aagatctgaa agaaatcggt cgattgatta
    10681 aaatcgatac gatgatccac agtgtgggtc attccgagcg gacaggtgtc gttgttgaac
    10741 ctcggctgtc tactcaatgg tttgtaaaaa tggcgccgtt agcgaaaaaa gccattgata
    10801 accaagaaac agatcaagca gtggaattct tccctccaag attcaatcaa acgtttttac
    10861 gttggatgga aaatgtccat gattgggtaa tctcacgaca attatggtgg ggacatcaaa
    10921 ttcctgcatg gtatcacaaa gaaacaggtg aaatgtatgt agggatggaa gcaccagcgg
    10981 atagtgaaaa ctgggtacaa gatcctgatg ttttagacac ttggtttagt tcagctttgt
    11041 ggcctttctc aacaatgggc tggccagata cggaaagcga agattataag cgatacttcc
    11101 ctactagcac tttagtaacc ggttatgaca tcattttctt ctgggtaagc agaatgattt
    11161 tccaaagctt ggaatttact ggcgaacgtc catttgagaa tgtcttgatt catggattga
    11221 ttcgtgacga acaagggcgg aagatgagta aatcattagg taatggaatc gatccgatgg
    11281 aagtaatcga aaagtatgga gcagatgctc tccgctggtt cttatcgaat ggttctgcac
    11341 caggacaaga tgtccgcttc agctatgaaa aaatggatgc ttcttggaac tttatcaaca
    11401 aaatctggaa tgcttcacgt ttcgttatca tgaatatcga aggaatgacc gtagaagaga
    11461 tcgatttatc tggagaaaaa tcagtggctg accggtggat ccttactcgt ttgaatgaaa
    11521 cgatcgaacg agtaactgag ctatttgatc gttttgaatt tggtgaagca ggtcgacaac
    11581 tgtataactt tatctgggat gatttttgtg actggtacat cgaaatgagt aaagaagtac
    11641 tttatggaga tgatgctgtt tcaaaacaaa ccacccgtag catcttagtc catacacttg
    11701 atcagatcct acgtttattg catccaatca tgccattcgt tacagaagaa atatgggaac
    11761 atattcctca tcagggaaat tctctagtag tggcagaata tcctgttgta cgtccagagt
    11821 ttgatgatga aacagcttct aaagggatgg aagtattgaa agaattgatt cgagccgttc
    11881 gaaatattcg ttcagaagtg aatacaccat tgtctaaacc aattacgttg ctaattaaaa
    11941 caaacgatgc taaaatcgat caatttttag ttgaaaatac aagctatatc gaacgcttct
    12001 gtaatcccga agagttgacg atatcaagtg agatcactgc gccagatcta gcgatgtcag
    12061 ctgttctgac aggtgcagaa atcttcttgc cgttagcagg attgatcaat attgaagaag
    12121 aaatcaaacg attagaaaaa gaactggaaa agtggacaag cgaagtaaaa cgtgtccaag
    12181 gaaaactttc taatgaacgt tttgtttcta acgcgccgga agaagtagta gaggcagagc
    12241 gtgcaaaaga aaaagattat ttagagaagc aaacggctgt caaagaacga atcgaaagtt
    12301 tgcgtactat tcaataagaa tagaaaaaga gagtgtgaaa tcagctagct attactaaaa
    12361 gctagctgat tgaacatcgt tacaggaaag agtttgagcc tcactcttag cttcgattca
    12421 taaggagatg tgacataaat tttgtcgcat ctctttttta gtctactctt attatgtagg
    12481 agaaggaggg aggtggatca tgtatcaaga gtcaataaag tagttgataa aatcgttcct
    12541 ctctctgttt cttttctctt tgtcatactt ttatttttct tatgtgttct atttgttata
    12601 ataaaagaga aatgggggaa ttgttgatga cgattgaaga agcaatagaa tggatccata
    12661 gtcgattacc atttggttca cgtcctggcc ttgaccgagt cgaagcttta ttagaagtgg
    12721 tagggcatcc agagagaaaa gtaaaaacga tccatatcgc tggaacaaac ggaaaaggtt
    12781 ctactgtagc ttatttgaga agcctattag aagaggctgg gctgacagta ggtacattta
    12841 cttcacctta tatcgaaacg ttcaatgaac ggatcgcaat caatggcaaa agcattccag
    12901 acaatgattt gatccagctt gtaaaaaaat atcagccact gatcaaacaa cttgatcaat
    12961 ttgaagcagt agctggtatt actgaattcg agacattaac agcaatggca tttgattatt
    13021 ttttgatgaa gaaagtggac gtagcgatta tcgaagttgg cttaggcggc ttattggatt
    13081 ctaccaatgt agctgtacca atgctgacgg gaataacaac gattggactt gatcataccg
    13141 aaatacttgg agatacaatt gaagaaatcg ctgcacaaaa agctggaatc atcaaacaag
    13201 gcgttccagt agtaacagga aatattgccc cagaagcttt tactgtcatc caacaaacag
    13261 ctaaagcaaa aaatgcaccg ctttatgtat acggggaaaa ttataaaata aactatctgc
    13321 atcctgatga gatgtgggga gaagtatttg aattttttaa tgaaatagga aagatccctc
    13381 aattaaccac tccgctttta gggcaacatc aagctgaaaa tgcaggagta gccatcgaat
    13441 tgtattttgt ttactgtcaa ttggaaaagc tgcctttccg agaaaaagat gttttgaagg
    13501 gattgaagaa atctcgatgg ccaggaagaa tggagcgttt gagtaacgag ccattagttg
    13561 ttttagatgg tgcccataat acgcatgcaa tgaaccggct tgttgagaat ttgaaaaaag
    13621 aattcaaagg atatcaaatc aacattttgt tttccgcctt acgaactaaa aatgttagag
    13681 aaatgctaga acgattgcag caagtaccaa atgtccatat ttatttgacg acctttgaac
    13741 atccaaaagc catcgaatta agtgaagtat cagatttagc aagtgagaga gtatcaatcg
    13801 tttcattgtg gcaattcgga ttagcagata tattggaaaa gatgacaaat gaagacgtat
    13861 tagttgtgac cggttcactt tatttcattt cagaagttcg ccatttatta atacaattag
    13921 gaggagatca tgaagtataa tgcagtgatt tttgacatgg atggtttgct gttcgacaca
    13981 gagttagtgt attacgaagc cacacaagta gtagcagaca agatgggttt tccttatgat
    14041 aaagacctgt atttaaaatt cttaggtgtt tcagatgaag aagtatgggc gaattatcat
    14101 atgattttct cggaatttgg caaaacaagt gtacaaagat tcatagatga ttcataccag
    14161 gaaacgcttg aacgtttttc gcaaggagcg gtacaaatga agcccggtgt gttggaactc
    14221 ctctcttttt tagaagaaca tcagattccc aaagttgtcg cttccagtaa ccaacgagca
    14281 gtcattgaac ttttattgaa gaagaacaat ctgtttgaac gatttgagac gattgtttct
    14341 gcagaaaatg ttcaacgagc aaaaccagac ccagaaattt tcttgttagc taatcagaag
    14401 ctaggtacag cgaagaaaga aacattgatt ttagaagatt cacaaaatgg tattttagca
    14461 gcttctgcgg ctgagatccc ggtagtcatg gtaccagatc tgcttccacc atcccaagaa
    14521 ttgatcaaaa aaacagctgg ggttgtttct tctttacatg aaatccctgc attgttaaaa
    14581 taaaaatcca tctcttttcg tatcttattt tagataagat agaaaggaga tgtttttttg
    14641 ggaaaacgat tgatcaaaga agtacctact agctctttac caagggaacg tatggaaatt
    14701 tatggcgcgg aagctttatc ggatcaggag cttttggcaa tcttactgcg taccggtcaa
    14761 cacccttata gtgtgatgtc gattgctgga aatctgttaa aaacgtttgg aggactggcc
    14821 tctttacgac aggctacttt gcatgagtta gaagagattc gagggatagg acgcgtgaaa
    14881 gcaatcgaaa tcaaagcctt gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga
    14941 gcggctccac cggttcgtac cagctacgaa ttggcccagc aattgattct ggaaatgaaa
    15001 gatcacaaac aagaacattt ggtttgtatt tatttggaca cgaagaatca agtgatctta
    15061 aaaaaaacag tgttcatcgg ttcgctgaat cagtcagtcg ctcatcctag agaaattttc
    15121 cattatgctg tacgttattg tgcagcaagg atcgttttag cgcataatca tccttctggt
    15181 aatgttttaa ccatagtata ataaataata gtagttaaag tagtttaaga ggagatatta
    15241 aagggatatt ttactttaaa taaaaattaa caaggtttat aatttgtagt agttaaagta
    15301 tttaaagagg tgggaacatg tgaaggttca aaggatagaa gtggagaata agccgtatcc
    15361 attgtattta ttactagata aagaatacca gctaatagaa ccagtaatga aatttattaa
    15421 atacttagat aatactggta agtctcctaa taccattaaa gcatactgct atcatttaaa
    15481 gttgctgtac gagttcatgg aacagagagg tgttattctt aatgatatta actttgagtt
    15541 gttagcagac ttcgtaggtt ggttgagata tccttcagca tcaaatgtaa ttgatcttca
    15601 gtcaaaaaaa gccataagag aagaaacgac agtgaataca attttaaatg tagttatgag
    15661 ttttcttgat tatttaagta gattaggaga atttaaatca attgatgtat ttaaacaagc
    15721 aaagggaaga aatttcaaag gatttttaca tcatgttaat aagggtagat accaaaagaa
    15781 tgtcttaaag ttaagggtta aaaagaaaca gataagaaca ttgagatcag aggaagttaa
    15841 gcaaattatt gatgcttgtc atacgaaaag agataaatta attttaatgc ttatgtatga
    15901 gggtggttta agaatcggtg aagtgttatc gcttaggctt gaagatattg tcacttggga
    15961 caatcaaatc catttaacac ctagagatgt taatgttaat gaagcttata ttaaattaag
    16021 gaaggaaaga acaatacatg tgagtaaaga acttatgtca ctttatacag attacttgat
    16081 atatgagtat agtgaggaat tggagcatga ttatgttttt atttccttaa aagaaggcta
    16141 ttttgggaaa ccactaaagt accaaagtgt tcttgatcta gttagaagaa tagttaaaag
    16201 gactggaata gaatttacat cacatatgct tcgccacact cacgcaacgc agctaattag
    16261 ggaaggatgg gatgttgcgt tcgttcaaaa gagattaggt cacgcacatg ttcagacaac
    16321 gttaaatacc tatgttcatc tttcagatca ggatatgaaa aatgagttta ataaatacct
    16381 cgagagaaag gagcataaga aatgaatgct tctagtaaaa ggaaaattat tagtcagagt
    16441 gagattagca aaaaaatagc tgtaatgaat gaagaaatgc aggggttttg ggctaataat
    16501 agttgggata taagaaaatg tccacatcct tctgccatag aattaagtaa gaatcctgct
    16561 ttaaggaatc gttgggttcg ttttgaacgt gttaaaaatc tgtggttaag aacagaattg
    16621 aaatattttt atttttacca tttaaacaat ggaatatgga atgcaaaaac tgtctggatt
    16681 agaaaaggaa cagtaattaa taaaatgtta gatttcttag atttaaagta tcctagcatt
    16741 acttcaatta ctgaagttcc tattgaaaaa gcaatgacgg agtatagaac ttatttgaca
    16801 aaacggggtg ttagaattac cactactaat tataagatta ctgctaatca agaaaaaaca
    16861 cctgtaaaag ctaattccta ctatgttact aatctaaaac aatttatgga gttttatgag
    16921 aacttttatt ttgatggaga ggagtgggat aaagacgttt gggatagacg taacttacct
    16981 ttgccagatg ataaggttaa cccaacacaa tatgaatata caattaactt taaagggttt
    17041 cggaatacat attttaaaca acttgtaaaa agatattgta agttgagatt gaacatggat
    17101 agcttttcct atgtaagtga tattgcccaa agacttaaag agttctttaa ttttctggac
    17161 atgaaattta aacaagttca gagagtacac caattaacga gagtggaaat tgaagcatat
    17221 ttaagtgaac taaacatgat gggaataaaa cctagtacaa taactgggag gatctctata
    17281 ttggaaggac tatttagtac ccttcttagg ctagaatggg atgatgttcc ttccaaaata
    17341 ttaatttatt ctgaggacta tccgaaaata ccaagagcaa aaccacgctt tatagatgaa
    17401 ttcgtcctag agcaattgaa cagtcatctt gataaattac ccgaatatat agctacgatg
    17461 actatgattg ttcaagaatg tggaatgagg ataagtgaat tgtgcacctt gaaaaaaggc
    17521 tgtctattag aggacaaaga tggagatttc tttttaaagt attatcaatg gaaaatgaaa
    17581 aaggagcata tagttccaat atctaaagag gtagctttac ttattaaagt tcgggaagat
    17641 aaagtttcag aggaatttcc agatagtgaa tacctctttc caagaaaaga tggatcgcca
    17701 ttaaaacaag aaacatttag aggtgagtta aataaattag cttatgagca aaatatagtg
    17761 gataaatcag gtgagattta tagattccat gcccatgcct ttcgccatac agtaggaaca
    17821 agaatgatta acaacgggat gccccagcat attgtgcaga aatttttggg gcatgaaagc
    17881 ccagaaatga caagcagata cgctcatatc tttgatgaaa ctctaaaaaa tgaatttact
    17941 aaatttcagg aaaaactggt taccaataat ggagatgtgc ttgatctaga tgaagataat
    18001 gaagtcgatg atgtagagct tcaatggttc aagaaaaata taaatgcaca agtgcttcca
    18061 aatggttatt gtagattgcc agtagtagca ggtggttgtc cacatgcgaa tgcatgctta
    18121 gattgcactc acttctgtac cagtaagcaa ttcttaccac agcacgaaga acagttagag
    18181 cgtacagaag agttattagc catagctaag gataaacaat ggcaaagaca agtagagact
    18241 aatagccgtg ttaaagagcg tttagaacaa atcattggaa gtttgacggg gtaattatca
    18301 atggataaac aagttagaaa tacaacagaa attgtacgtt tggcgaagca gaaatcaaaa
    18361 aagacaaggg aaaaagtaga caaagcgatt tctaaatttt cgattgaagg taaagttatt
    18421 aattttaatt caatagcaaa ggaagctaat gtttctaaat catggcttta taaggaacac
    18481 gatattaggc aaagaatcga atcccttcgt gagcgtcaaa taacagcaaa tgtagtctca
    18541 aaacccaaga aaagttctcg ttcggaggaa atccttatta aaaccttaaa aagaagagta
    18601 atggaattag aaaaagaaaa taaaaaatta cagaaccaaa ttcaaaaatt atatggagat
    18661 ctgtataata aagaataatt attaatctgt agacaaattg tgaaaggatg tacttaaacg
    18721 ctaacggtca gctttattga acagtcattt aggtatatgt ccaatctagg gtaagtaaat
    18781 tgagtatcaa tataaacttt atatgaacat aatcagttac gaggtgaaat catgagcaat
    18841 ttgattaacg gaaaaatacc aaatcaagcg attcaaacat taaaaatcgt aaaagattta
    18901 tttggaagtt caatagttgg agtatatcta tttggttcag cagtaaatgg tggtttacgc
    18961 attaacagcg atgtagatgt tctagtcgtc gtgaatcata gtttacctca attaactcga
    19021 aaaaaactaa cagaaagact aatgactata tcaggaaaga ttggaaatac ggattctgtt
    19081 agaccacttg aagttacggt tataaatagg agtgaagttg tcccttggca atatcctcca
    19141 aaaagagaat ttatatacgg tgagtggctc aggggtgaat ttgagaatgg acaaattcag
    19201 gaaccaagct atgatcctga tttggctatt gttttagcac aagcaagaaa gaatagtatt
    19261 tctctatttg gtcctgattc ttcaagtata cttgtctccg tacctttgac agatattcga
    19321 agagcaatta aggattcttt gccagaacta attgagggga taaaaggtga tgagcgtaat
    19381 gtaattttaa ccctagctcg aatgtggcaa acagtgacta ctggtgaaat tacctcgaaa
    19441 gatgtcgctg cagaatgggc tatacctctt ttacctaaag agcatgtaac tttactggat
    19501 atagctagaa aaggctatcg gggagagtgt gatgataagt gggaaggact atattcaaag
    19561 gtgaaagcac tcgttaagta tatgaaaaat tctatagaaa cttctctcaa ttaggctaat
    19621 tttattgcaa taacaggtgc ttacttttaa aactactgat ttattgataa atattgaaca
    19681 atttttggga agaataaagc gtcctcttgt gaaattagag aacgctttat tactttaatt
    19741 tagtgaaaca atttgtaact attgaaaata gaaagaaatt gttccttcga tagtttatta
    19801 atattagtga catttgcatg cttcaaagcc tgtcggaatt ggtttttagt gaaaagaaca
    19861 cgatattcac ggtttaccca cttataaaca aaagatcgat actttttgta gtccttcttt
    19921 gaaatcaatg gttgatgtcg ttcaagaaca atcaatacag agtctacact tggcttagga
    19981 tgaaaatata gtggtggtac ttttttgagc atttttatat ccatctccac cattaatagt
    20041 aaacccaaag ctcgttgcag attttgcaat cttttcgcaa atcccttctc aacgataaga
    20101 tagctatatt tagcctgact ttcaaaggta attcttttga caatatccgt actgatgtta
    20161 taaggaatat taccatatat cttatagttt atatgttttg ggaaggaaaa ttttagaata
    20221 tccgtttgaa tcacttttat attctcagag gggtttaccg cttctttagt cacttgacat
    20281 aagcctccat caatttctat agcagtaact gatcgactca ttttgactag ctctttggta
    20341 aaatgtcctt ttcctgatcc gatttctatt acgttgtctt gtttactgat attcgtgtga
    20401 ttcaatattt cttttacatg ctttttagaa gtaataaaat tttgcgtgtc tttagggttt
    20461 ttctggttca ttataacctt ctccttactg gttataatga actgacatta agcgttcatt
    20521 ataaccaatt aattttgatt tggttgataa cgaactttat taataacaaa tatagaaaaa
    20581 gtacccatac ttttatctcc tttccatttt tttcattatg aatgagataa agtaatttct
    20641 actactgcga tactggtgca cataattgaa acctccttat gtaactgaat atgattgtat
    20701 catcttttca ttttagaata aacctttata tgtaattttg cacttgacac ttcagatgga
    20761 cgttttcttc aaaataatat gttttgaaac ttgataagag aagccactgt ctttactaaa
    20821 aagtaaaggc tgcgataaag gacagtgact ttaataaagt tgcactgttg tattgtcttt
    20881 ggataaaatt gtctaaaggg aagttttatc cccaacgaaa aaacatctaa agtggtaagt
    20941 aatgaaagaa tgtcgataaa gtaggaatgg gggatctttt tgaatacaat agaatcacag
    21001 tttgataagg ttgcagaaga ttacgatttc gtgaatgagc ttttgaatga ttattcattc
    21061 tttgtgtcta atatgtctcc aaagaaaggc agagcattag atatcggatg tggctcgggt
    21121 ttgttagtgg agaaattagc aagttattat gatgaagtgg tagggattga tatttctaat
    21181 caaatgctcg atcttgccaa atctaaacgt caactaacaa atacggtcta tctgaatatg
    21241 aatgcagaac aacttaattt taatgagaag tttgatttta ttgtaagccg aacaaccttt
    21301 catcatttgg atgatatagc cagcgtaata cagcaaatga aggaactgtt gaatgaggaa
    21361 ggaagaatag ttattcttga taatgtatct gaagtcgaga caccgcctac ctatgtttat
    21421 aaattagggg caatccaaga gtttttaccg cattgtttca aatttggaat aaaaaatgcg
    21481 attagaattt ataatcacaa cacatctaaa tcatggcttg agcatttagc ttcagataag
    21541 tacctatctg aacaaaatta ttacgattta tatgaaaagt tgttgcctgg atgccaattc
    21601 cataaaatgg gttgggcaat gggggttgtc tggacaaaat aacttaaagc tgtggctttg
    21661 aaaagttgaa tttgtgattg ttttcagagc tatcgggtgc tttagtataa ataacaatga
    21721 ataaaaaatc agcctgtatc ttatgttact attaagtaat ttaagataca ggttttacta
    21781 tttttgaaat aagtaccttc cacttcaaat acatgtattc aaagtgaaag tattgattta
    21841 aatgattctt ttgtaattta ctcttgataa tatgaaattc catcgcagca ggatatgaat
    21901 tttaccaaaa ggatacaaaa gtgtggagaa atgatgggga tcactgtact ggaccacttg
    21961 atcatcggac gcaaacgata ttttagtttg agagaagaag gaatgatgga agagaagtaa
    22021 gttgctttct tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt ttgtttggga
    22081 atagaagttg aaacatctgt tgcacctcta tcgtacacga gcaatgttac aatttaaatt
    22141 agtgcttttg gcacgaggag gatattatta tggaaaacgg aacagtaaaa tggttcaacg
    22201 ctgaaaaagg atttggattt atctcacgtg aagatggaag cgacgtattc gttcacttct
    22261 cagctatcca aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg actttcgacg
    22321 ttgaagaatc agaccgcggc ccacaagctg caaacgttgt aaaagcttaa ttttatctgt
    22381 aaatcaaatt gatggtagac atcaattttt acgagagaac aaagcgagta acaaaaatac
    22441 ctgccacaag agacaatttc tcttgtggca ggtatttttt tgtatgaaaa agtaaataaa
    22501 tttaagaggc tatgaaagag ccaccttgta tcaaggaata agaacaaaca aaaatggctt
    22561 ctcatgtttt tcatttgttt tctcttatat tcaggacaag gtgctgattt cataacctct
    22621 tatttattct tcatctggtg tcattaccat tggcaaaatc attggatgtc tttctgtacg
    22681 ttcgaataag aaaggctgaa gcgcgccagt catcgcttct ttcaatttat attctgaaca
    22741 gtcaggtcca ttcaaacatt ctcttaaagc gtggaataag attcgctgag cttcattgat
    22801 catttctcct gattctcgca tgtagacaaa tccacgagag agaatatctg gaccagccaa
    22861 gatttctttt ttcttgatat ccactgtcgc gacagctaat acaagacctt cttctgaaag
    22921 caggtgacgg tcacgtaaaa cgacattacc aatatcaccg atcccgctac catcgatata
    22981 cacatctcca gccacaaaat gcccagcagg acgcgcagaa tcgctagtta acgctaaaac
    23041 atcgccattt tccatgatga agcagttttc tgcaggaaca cctacatctt gtgctagaga
    23101 agcatgtact ttcaacatgc ggaattctcc gtggacagga ataaaatatt ttggcttcat
    23161 caaacgaagc attaattttt gttcttcttg tccaccgtga ccggaagtat ggatgttatt
    23221 gatttttccg tgaatcactt ctgcaccagc ttcagacagc aaattgatca aatgattgac
    23281 acttgttgta ttaccaggaa taggtgaact tgaaaagatc actgtatcgt ctggctggat
    23341 ttggatttga cggtgagtac cattcgcgat gcggctcaac gcggccatcg cttctccttg
    23401 agaacctgta cataggatca tcgtttcatt ggcaggcaag cgattgattt cgtgtgcatc
    23461 tacaaatgtg ccgtctggaa ctttgatata tcctaaacgt tgaccgttga cgatggcatt
    23521 ttccatgctt cgtccgaaaa cagcgatttt acgaccagtt gccacagcgg catctgctgc
    23581 ttgctgcaaa cggaagatgt tagaagcaaa gctggcaaaa atgatacgtc cttcgatttt
    23641 tgaaaagatc ttcaaaatag aatgcccaat cgttttttct gatttagtaa atgttggaat
    23701 ctcagcattt gtactatcag ataaaaggca caagactcct tcttccccga tacgagccat
    23761 tttatgcaag tttgctggtt cgccaacagg agtgaaatcg aatttgaagt ctccagtcgc
    23821 tacgatattt ccagaaggtg ttttcaccac gatccctaat gcatctggga tactatgggt
    23881 cgttctgaaa aagctgacag aagtctttct aaaacggatg atcgtatctt cattgatctc
    23941 atggagctct gcatctcgaa gcaagccatg ctcatctaat ttgtttgtga tcaacgctaa
    24001 tgcgagagga ccagcataga taggaatatt tgcttgtttc agcaagtagg gtattccgcc
    24061 gatgtggtct tcgtgcccgt gagtaatcac caaggctttt actttgtgta agttttgtat
    24121 gatgtagctg tagtcgggaa tgacatagtc aattccaagt aaatcgtctt ccgggaattt
    24181 gatcccggca tcaatcaaaa tgatttcatc ttggaattgt accccatacg tatttttccc
    24241 gatttcacct aatccaccaa tagcaaagac gcctgtctcg ttatttttga tggaaagttt
    24301 catattaaaa ctccactaat gagaaatcag cattttcttt ttcgtactct agatgacttc
    24361 cttctagaag ttgaacgaat tcaatattgt atggggtatt ttcttccaca cgttggcgag
    24421 cgtctacatc gctttttgcc tcaatgtaaa gagattttgt ttcttcccgt ttagggttgc
    24481 gtacttttgt ttcttgataa taaactttat agatcattgt tttgtctcct ttatctgcgt
    24541 aaagcagtga tttagctagt tgattttttt agacaacaca aaacgagggg acaagccacc
    24601 tggtttttca tatgctttga ctagaatact ggattcgagt caaagagaca agcctcgttt
    24661 ttgcttatct gttcggatat tttttcaacc gtaccttctg tataaaaaat atacaacaat
    24721 taattgttat tctagcataa aacagcaaat aagtatagaa atcaactaga ttgaaagaaa
    24781 aaatcaatgt ttatcatccc gctataatca taatgacttt tttatgagag aagtgttcta
    24841 ttaataggct cgttgtgcta tcatttttag taatgaaaat gtaacaaaag attaaaagga
    24901 gatgagaaag tgaaactaat gtggcattat accatgcgtt ataagaaatt cttgtttttc
    24961 aatttcatat gcgtatttgg ttttattttg attgaattag gacttcctac gattttagca
    25021 cgtatgatcg atgtaggaat ccgcaatgat gattttgatt atgtgaagca acaagggata
    25081 tttatgatcg ttattactgt aatcggtatt attttgaata tcttgttagg ttattttggt
    25141 tcgaggatta cgactaatat cgtagcagat attcgagatg atttgttcga aaagatccaa
    25201 agttattcgc atcaggaata cgaaaatcta ggcgtatctt cattgatcac tcgtacaaca
    25261 aacgatgctt atcagatcat gttgttcctg caaaatattt tacgtatcgg ttttatggca
    25321 ccaatgatgt tcattgtcag tttgtacatg gtcatgcgta caagtgtgac actcagtttg
    25381 tatgtagtag gtgcattacc tttactatta ttggcagtcg tagcgattgc taaggtgtct
    25441 gaaccgttat ctaaaaagca acaaaaaaac ttagataaaa tcaacagtat cttgcgggaa
    25501 aatctttcag gattgcgtgt gattcgtgca ttcgtcaatg aaaagtttga agaaaaacgt
    25561 tttgctgtag tcaatgagaa ttatgcttct agttcaaaaa gcttattccg tttgatggct
    25621 gttgcacaac cgggttttta tttcttattt aatatcgtaa tggtattgat catctggagc
    25681 ggggcagtgc aaatcgatgc aggcgagctt cgtgtaggag atttgattgc gtttatcgag
    25741 tatatattcc atgccttgtt ctcattcatg ttatttgcta gtgtgttcat gatgtatcca
    25801 agagcagctg tttcagctgg acgtatccaa gaagccctag atgccaaacc ttcgattgta
    25861 gaagatccaa atggtatatc tgatacaaaa actaaagggt atatcgaatt tcaaaatgta
    25921 acatttgctt atccaggcca ttcgcaagag ccagtgatac gaaatgtcag ttttacagcc
    25981 tctccaggag agaccgttgc ttttattgga agtaccggta gcggaaaatc tacgttgatc
    26041 caattgatcc ctcgctttta tgatgtgagt gaaggaaaga tcttgatcga tggagtagac
    26101 gtgagagatt atcggctaag caagttgcga gataagattg ggtttattcc gcaaaaagcg
    26161 ctgctattta ctggaacgat tgcagataat ttgcgatacg ggaaagaaga tgctacgcaa
    26221 gaagaaatgg aacgtgcagc tgagattgct caagcttcag aattcatttc tcgaaaacca
    26281 gatggattta atgaacattt atcagaaggt ggttccaatt tttcaggtgg acaaaaacaa
    26341 cgattggcaa tagccagagc catcatacgc cgtccggaaa tctatatttt tgatgacagc
    26401 ttttctgcat tagattatca aacggatgcg aagttacgtg ctcgtctgaa aaaagaaacg
    26461 acagaatcta ctgtattgat cgtagcacaa cgtgtgggaa cgatcatgca tgcagataag
    26521 atcatcgtat taaatgaagg agaagtcgtc ggtatgggga cgcatcggga gttgttggaa
    26581 aaatgcccga tttattatga tattgccgct tctcaattat caaaggagga attagcatga
    26641 aaaaaacctt ttcttcctta aaacgtttag ctcgatatat cagaccgtat cgtgagactt
    26701 ttcttttagt gatcctattt acagttctga cagtggcttt caacgtggct ttgccatatg
    26761 ttacaggact tcctactaca gaaatcagcc gtaatattgc caatggcgaa tcaatcaatt
    26821 tttcttatat attcaaatgt ctcatttgga tcacagttgt tggaataggg tattgtattt
    26881 cgcaattact ttctggtgtt ttgatgacaa atgtcgttca aagtgccatg catgatttga
    26941 gaagagacat agaagaaaaa atcaatcgtc ttccggtttc atatttcgat aaaaaccagc
    27001 aaggaaatat tttgtctcgt gtgacgaatg atgtagatgc agtcagcggt gctttacaac
    27061 aagcttttat tggtatcgta aatgcgatat tgggaattac gttggctgca gcaatgatgt
    27121 tttatatcca gccgatgatg gcgctgatct ctatgatcat gattccaaca tcgatctgga
    27181 tctcaaaacg agtgatcaat gcttcgcaga aatattttca aagcatgcag aattctcttg
    27241 gtgagctaaa cggctacgtc caagaaaata tgacaggttt tagtgtattg aaagtatatg
    27301 gacgcgaaaa agaaacattg gaaggattca aaaaagttaa tcacagcttg aaatactttg
    27361 ggtttcgtgc agcattcatt tctggcttga tgatgcctct agttcaatta actgcctatg
    27421 ctacttatat tggtatggct gttctaggaa gcttttatgt tattactggg gcgatcgttg
    27481 tcggtcaact acaagcattc atccaatata tttggcaaat cagtcagcca atggggaacg
    27541 tgacacaatt gtcttcagtt ttgcaaagtg catcagcggc aacgaaacgt gtattcgaga
    27601 ttttagatga gccagaagaa aaagaaaaca aagtagatgc ttcactgcca gaaacgattt
    27661 taggagatgt gacattcgat catgtcgatt ttgcatatga tccgaagaaa ccattaatcc
    27721 aagatttgaa ttttgaagtg aaagctggtc aaaccgtagc tgtggtcggt ccgaccggtg
    27781 ccggcaagac gacattgatc aatctattga tgcgttttta tgacgtagac aaaggagcaa
    27841 taaggattga tggaatcgac accaaatcaa tgagtcgcag tgatgtgcgt tcgctatttg
    27901 gtatggtctt gcaagatgca tggctttatc aaggaacgat tgcagataat atccgcttcg
    27961 gaaagcttga tgcgacagat tatgaagtag ttgatgctgc gaaaacagca aacgtagatc
    28021 attttatccg aacgatgcca gatggatatg atatggccat caattctgag ggagacaatg
    28081 tgtctttagg acaaaaacag ttgctgacga ttgcccgagc agtggtctcc gatcctaaaa
    28141 tcttgatttt agatgaagca acaagttcag tagacacccg tttagaagcg ttgattcaaa
    28201 aagcaatgga tcgcgtgatg gagggacgga caagtttcgt catcgctcac cgtttatcga
    28261 caatccgtga tgcagatctg attctagtga tggatcaagg tcagatcatt gaaaaaggaa
    28321 cgcatgagag tttattggca caaggtggat tttatgaaaa actgtataac agccaatttg
    28381 ctgaggaagc agaataagtc atataagaat aaaacagttt aaaacttatt ttttgttctc
    28441 tattcttgat aatggtaaag taaagttatc aagaaaagtg aggacacgga gatggaacag
    28501 tttttaaatg aaaaaattac tgaagccaaa agagaagttg atcgtttaac gcacgtctgt
    28561 cagacgaagc cttttgattc agctgatttt tttgaaaatg agtttcaaat cgaacgcttg
    28621 gcagcacaaa tagaagtgta cgaattaatt ttaaaaaaag taatagaaga aaatatagat
    28681 gtacaataaa aacaggtaaa acgtcagtta ccagatactg taaatagaca tctggcctga
    28741 gcaggtttta cctgtttttt ttcttatgaa ataaatatat gctatgatac ctttattcaa
    28801 taggattagt ttctacaaaa aatgaaagga gaaaaataaa tatggaacgg tttatagcat
    28861 caaatgccac agtgattggt gatgtgacat tgtcagaaga tgtgacgatc tggtatcaag
    28921 ctgttttacg cggggacagt aattggataa aaataggaca aagaacgaat atccaagatg
    28981 gtacaatcat ccatgtcgat catgatgcac ctgtagatat tgcggagaat gtgacggtag
    29041 gacatcagtg catgctacat ggctgtacaa tcgaaaaagg ggcgctgatc gggatgggga
    29101 ccacgatatt gaatcatgcg gttattggtg agaatagttt gattggtgct ggatcattag
    29161 taacagaagg aaaagttatt cctcctaatg tcttggcttt tggtcgccca gctaaagtga
    29221 tccgtccttt gacaaaagaa gaaatacaaa aaaacaaaga aaacatccag cattatgtcg
    29281 agataggtcg gatgcatgcg caaggaaaat tcaaggagca aagataggca gtagcatcca
    29341 taaaataaaa aaggatggtc agtttgagtt gaccatcctt ttttatttgg tttagataag
    29401 agagcttaaa aaagcttcct ttgaaacaga ttaatttaat gaagaactgt tttccatgac
    29461 ttcatcgatc aatccatatg cttttgcttg ttcagctgtc atatagttgt cacgatctgt
    29521 atctttttca atcacttcaa gcggttgtcc agtgcgttca gctaaaattt tgtttaagcg
    29581 ttcgcgtgtt tgcaaaatat ggcgtgccgc aatttcgatt tcagttgctt gcccttgagc
    29641 gccgccaaga ggttgatgga tcatgatctc agcatttggt aaagcaaatc gtttaccttt
    29701 tgtaccagct gtcagcaaga agcttcccat tgaagcagcc atacccataa caattgtttg
    29761 gacatctgct ttgacaaagt tcattgtgtc gtaaatagcc atacctgctg taactgaacc
    29821 accaggagaa ttgatataaa gatagatatc tttttcagaa tcttgagcgt ctaagaaaag
    29881 caattgggcg atgatcgaat tagcaagatc atctgttact tggccgctta acatgatgat
    29941 acggtctttc aataaacgtg agtagatatc gtaagctctt tcacctcgtg aagattgttc
    30001 aatgactgta ggaattaaat tcataataga gttcctcctt gatttcgttt agaaatagtg
    30061 taccataatt tagtttctaa tacctaaaat gtagtataca tttttggtca aaaaaggtca
    30121 aataaaaaac ttaggcaaat tagtcgcata aacccgcttt atggtatgat aagcagagaa
    30181 acaaaacaag gaggaacaac agattgatag ttggtattac tggccttatc ggagtgctga
    30241 caggtgctgt agttgtctat atcggcatga tactgcaaat gcgtcacttg gaaaataaag
    30301 aacagcgtag acgtgaattg gaattgaaag tcaaagaaat cgagacgctg aatcaattaa
    30361 ataaaaagat caacgagatc ttgcaaaaac ggacaacgct gatggaagaa tatgtcagct
    30421 ttgatgcatt tgatgattgt tatatcacga ttgacgattt tgcgtatctc caatcatttt
    30481 cagcgcaaaa tagtttttat ctgccaaatt atattttaga cgagctattt aagaatatct
    30541 cccatcgaaa agtgatttta tctccagatg aaacaatgaa gattggtgga tatgcttata
    30601 aaggcggacg cgtgatttta gagaatttta cggatagtct gactgaaatg atcaatgaaa
    30661 aaaaagctca gttgaagact atgacgcgcc agccgattgg tttcttctcg aaagatgatg
    30721 tttggagctg atattttgaa gcattgccgt tgcacatgag agcgtggtaa tgctttcttt
    30781 ttttcttaat gatacaatag aactgaacgg agtgaagaaa gatgttacaa atctatgccc
    30841 atcgcggaag cagcggcacc cacccagaaa atacgcttcc tgcatttgca gaagctgtac
    30901 gagtaggtgc agatggtatc gaactagatg tccatctaag caaagatggt tatttgatcg
    30961 ttatgcacga cgaagaagtg gaccggacga ctaacggtaa agggctgatt cgagaaaaaa
    31021 cattggaaga actcaaaaaa ttgaatgcag ggggcaaatt cagcaaagaa tttccagcag
    31081 ctaaagttcc tgaattgaga gaagtgttag gactgctcct ccaaaaaaat taccgaggga
    31141 tgctaatgat tgaaattaaa actgatcagt atgaatatcc cggtatcgaa gaaaaattag
    31201 ccgaaacatt aaaaagtaga gattggcctt ttcaacatgc atattgcagc tttcatttgg
    31261 attctttgga aaaaatcgca ttactggaac cggagataca gctggactta ttgatgagta
    31321 cttctgataa aaagatccaa ctagcagcag aaacccctta tatagaagga gtacatccaa
    31381 aaaatacttg gttgttcgaa catccagtcc aggcagctga ttttccaaaa gcaattcgtg
    31441 catggacgat taacgatgaa gaagaaatgg ccaaatgtca agaactcaaa atagatggaa
    31501 taataactga ttttccggag aaagctcttc ggtggaaaca atcaacgaaa agtaggtaaa
    31561 atatggaaaa agtattagtg atcgtcggtc cgacagcagt cggtaaaaca gcgctaagtg
    31621 tggaactcgc acaactattt ggaggagaga tcatcagtgg tgattcattg caaatctata
    31681 aaaatctgga tatcggtaca gcaaaaatca aagaagaaga gatgaataat gttccgcatc
    31741 atttgattga tgtgatcgaa cctactcaac gttactcagc cgctgatttt cagcattcag
    31801 gtcgacaatt gattaccgag attgcgaacc gagggcacct acctatcata gctggtggta
    31861 cgggtctcta tatccaatcc cttctatatg attatcagtt aggagcgaca gaagaagcga
    31921 atcctgagat tagaaaaaag tatgaaacgt tagctgaaac aattggttcc caagcattat
    31981 ggaatcagtt gaaagaaaaa gacccggcag ctgctgagaa aatccactgg aacaatcaac
    32041 gcaaagtgat aagagctctg gaagttttag agacgacagg acatagtatc acggcaccta
    32101 aagaagagcc aaagcaattg tatgattacc tcatgattgg attagatacg aagcgtagta
    32161 ttctttatga acgaatcaat caaagagtcg atttgatgct agcagaaggt ttagtagcag
    32221 aagcgcgtaa ggtgtatgag cttggagaag tccaggccag tcaaggtatt ggatataaag
    32281 aatttttccc ttattttgaa gggaaagaaa cattggaaga agcaacagaa caagtcaaac
    32341 tacattcgag aagatacgcg aaacgtcagt tgacgtggtt tcgtaaccgt ttggatgcac
    32401 agtggtttga tcttataagt gagccaaatc aaaaaagtca gattgagcaa acaatcaaaa
    32461 aatggctgga ggcggaataa aatggaagaa aaagtgatca tcgtaggggt agaaaccgaa
    32521 gcaaaccagc gttacttttc agagtcaatg gaagagctag ttcagctgac gaataccgca
    32581 tcaggagaag tcgtattcac aattacacaa aagagaccac aagtggatcg gcaaacgatt
    32641 atcggtaaag ggaaattaca agaattagtt caacaagcgg atgcgcatga agcggatttg
    32701 atcatcttta atcatgagct aactccgaga cagagccaac tgattaccga tgctgttggt
    32761 cttccggtaa ttgatcgcgt gcagcttatc ttagatattt ttgcgatgcg tgctcgttcg
    32821 aaagaaggga agctgcaggt tgaacttgct cagttagaat acctgctgcc tcgtttagta
    32881 gggcaaggga aaacattatc tcgtcttggc ggtgggatcg gaacaagagg gccaggtgaa
    32941 acaaagttag aaacagaccg ccgacatata cggaataaaa tttttgctgt taagaaagaa
    33001 ttgaaagaag tagaagctca tcgagagcgc aatcgccaaa aaagaaaaaa tagcgagatt
    33061 tttcagattg gacttattgg ctacacgaat gccgggaagt caactatctt gaatctattg
    33121 acacaagcgg atacttattc gaaagatcaa ttgtttgcaa cgttagatcc attgactaag
    33181 agatggcgtt ttgctgaagg atttgagatt actgtaacgg ataccgtcgg gttcatccaa
    33241 gacctgccta cgcaactgat cgatgctttc cattctacat tagaagaaag tcaaaatatg
    33301 gatcttttgc tacatgtagt cgatgctagt tctccagatc gtattttaca ggagcagact
    33361 gtcttaaaat tgatggatga actgaatatg aaagaaatgc cgatactgac tgtttacaat
    33421 aaggcagacc agatcgaccc agcgatgttc acgcctacgc tattcccaaa tgtattgatt
    33481 tctactcaga cgaaagaagg taagatggcg ttgatcgaag caatcaaacg tcagctgatg
    33541 gaactaatga ccccttatac actttctgtg ccaagcaacg aaggacagaa attaagcgag
    33601 ttgagaagac aaacaatggt tttggacgaa gcgtacatgg aagaaacaaa cgaatatgaa
    33661 gttaaagggt ttgcaaaaac caattcaaaa tggttaagaa aaaaccaata aaattttatt
    33721 tttttaa
//
LOCUS       DANKAZ010000022        32896 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000022 DANKAZ010000000
VERSION     DANKAZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            348..1037
                     /locus_tag="PWY67_000987"
     CDS             348..1037
                     /locus_tag="PWY67_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2057288.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1024..2232
                     /locus_tag="PWY67_000988"
     CDS             1024..2232
                     /locus_tag="PWY67_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2057289.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2526..3797
                     /gene="serS"
                     /locus_tag="PWY67_000989"
     CDS             2526..3797
                     /gene="serS"
                     /locus_tag="PWY67_000989"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL2057290.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4346..5830)
                     /gene="guaB"
                     /locus_tag="PWY67_000990"
     CDS             complement(4346..5830)
                     /gene="guaB"
                     /locus_tag="PWY67_000990"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL2057291.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6335..7030)
                     /locus_tag="PWY67_000991"
     CDS             complement(6335..7030)
                     /locus_tag="PWY67_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL2057292.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7120..8220)
                     /gene="ychF"
                     /locus_tag="PWY67_000992"
     CDS             complement(7120..8220)
                     /gene="ychF"
                     /locus_tag="PWY67_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL2057293.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8229..8420)
                     /locus_tag="PWY67_000993"
     CDS             complement(8229..8420)
                     /locus_tag="PWY67_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL2057294.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8441..9331)
                     /locus_tag="PWY67_000994"
     CDS             complement(8441..9331)
                     /locus_tag="PWY67_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL2057295.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9318..10085)
                     /locus_tag="PWY67_000995"
     CDS             complement(9318..10085)
                     /locus_tag="PWY67_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2057296.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10097..10810)
                     /gene="rsmG"
                     /locus_tag="PWY67_000996"
     CDS             complement(10097..10810)
                     /gene="rsmG"
                     /locus_tag="PWY67_000996"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL2057297.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11042..12943)
                     /gene="mnmG"
                     /locus_tag="PWY67_000997"
     CDS             complement(11042..12943)
                     /gene="mnmG"
                     /locus_tag="PWY67_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL2057298.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12960..14357)
                     /gene="mnmE"
                     /locus_tag="PWY67_000998"
     CDS             complement(12960..14357)
                     /gene="mnmE"
                     /locus_tag="PWY67_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL2057299.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14656..15039
                     /locus_tag="PWY67_000999"
     CDS             14656..15039
                     /locus_tag="PWY67_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057300.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15207..15965)
                     /locus_tag="PWY67_001000"
     CDS             complement(15207..15965)
                     /locus_tag="PWY67_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL2057301.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15983..16801)
                     /locus_tag="PWY67_001001"
     CDS             complement(15983..16801)
                     /locus_tag="PWY67_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2057302.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16798..17154)
                     /gene="rnpA"
                     /locus_tag="PWY67_001002"
     CDS             complement(16798..17154)
                     /gene="rnpA"
                     /locus_tag="PWY67_001002"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL2057303.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17272..17406)
                     /gene="rpmH"
                     /locus_tag="PWY67_001003"
     CDS             complement(17272..17406)
                     /gene="rpmH"
                     /locus_tag="PWY67_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL2057304.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17929..19263
                     /gene="dnaA"
                     /locus_tag="PWY67_001004"
     CDS             17929..19263
                     /gene="dnaA"
                     /locus_tag="PWY67_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL2057305.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19461..20591
                     /gene="dnaN"
                     /locus_tag="PWY67_001005"
     CDS             19461..20591
                     /gene="dnaN"
                     /locus_tag="PWY67_001005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL2057306.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20820..21062
                     /gene="yaaA"
                     /locus_tag="PWY67_001006"
     CDS             20820..21062
                     /gene="yaaA"
                     /locus_tag="PWY67_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL2057307.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21049..22173
                     /gene="recF"
                     /locus_tag="PWY67_001007"
     CDS             21049..22173
                     /gene="recF"
                     /locus_tag="PWY67_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL2057308.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22170..24116
                     /gene="gyrB"
                     /locus_tag="PWY67_001008"
     CDS             22170..24116
                     /gene="gyrB"
                     /locus_tag="PWY67_001008"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL2057309.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24136..26607
                     /gene="gyrA"
                     /locus_tag="PWY67_001009"
     CDS             24136..26607
                     /gene="gyrA"
                     /locus_tag="PWY67_001009"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL2057310.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26843..27142
                     /locus_tag="PWY67_001010"
     CDS             26843..27142
                     /locus_tag="PWY67_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL2057311.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27192..27704
                     /gene="ssb"
                     /locus_tag="PWY67_001011"
     CDS             27192..27704
                     /gene="ssb"
                     /locus_tag="PWY67_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2057312.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27730..27966
                     /locus_tag="PWY67_001012"
     CDS             27730..27966
                     /locus_tag="PWY67_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL2057313.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28109..30082
                     /locus_tag="PWY67_001013"
     CDS             28109..30082
                     /locus_tag="PWY67_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL2057314.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30088..30540
                     /gene="rplI"
                     /locus_tag="PWY67_001014"
     CDS             30088..30540
                     /gene="rplI"
                     /locus_tag="PWY67_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL2057315.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30814..32181
                     /gene="dnaB"
                     /locus_tag="PWY67_001015"
     CDS             30814..32181
                     /gene="dnaB"
                     /locus_tag="PWY67_001015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL2057316.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT        10007 a         6425 c         6475 g         9989 t
ORIGIN      
        1 caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct tttgaacaaa
       61 taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt atcaacattt
      121 ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta taactccgag
      181 agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat tcacaccaac
      241 tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct atttctgctt
      301 ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg aaaattttga
      361 ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac aatgaggggt
      421 atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga acaatccctg
      481 atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg caagtagtga
      541 aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa actactgata
      601 tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag ccatttaatc
      661 cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg cagattgccc
      721 cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac tctcatgaag
      781 ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt atcctctact
      841 tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag cgtgtttgga
      901 aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat ttaagagata
      961 agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga gtgggctata
     1021 aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa tttgacttca
     1081 aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt gcttctatta
     1141 aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc tttgacgaat
     1201 gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt gttctggaac
     1261 ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt gtattggcgc
     1321 ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact tcactacatc
     1381 gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt aagccgagtg
     1441 gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga cgaacgtaaa
     1501 atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg taccccactc
     1561 acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc agaagaagat
     1621 cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc tttagtggac
     1681 gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt ttctgccttt
     1741 gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc ttctaaaaaa
     1801 aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg cgataccgaa
     1861 aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg aaaaggagca
     1921 acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac agtcaaaaat
     1981 aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt ttatcgtgta
     2041 gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat cgctcaaagc
     2101 attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg gacttctttc
     2161 atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca ggaagttatt
     2221 gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg aaaaacacag
     2281 aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc agcaaaggat
     2341 aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc ggttcaacag
     2401 ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc gaatttcaga
     2461 ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa aaaggaggaa
     2521 atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa gaaaaattga
     2581 tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat gaaaatcgtc
     2641 gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt tctgctgaga
     2701 tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa atgaaagaag
     2761 tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa aaattgaaac
     2821 aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga acagatgaag
     2881 aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt gaaccaaaac
     2941 cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc gcaaaagtag
     3001 ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt gctgtctaca
     3061 acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc actccttata
     3121 tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa gaagatgttt
     3181 tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca ttaactaact
     3241 attataatga tgaaatttta gatggtgcag atttacctat ttatttcact gcactaagcc
     3301 ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg attcgtcttc
     3361 accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct tacgaagaat
     3421 tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg ccttatcgtg
     3481 tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat gacttagaag
     3541 tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac tgtgaagatt
     3601 tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc cactatactc
     3661 atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt ttggaaaact
     3721 accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat atggggaatc
     3781 tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta ttaagaaaaa
     3841 ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg agaaactggc
     3901 ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag tattagagaa
     3961 aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc gagggtcagc
     4021 tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct agttacaagg
     4081 acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat tcggaggtgg
     4141 caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact ccaagaaata
     4201 agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta tttccgaagg
     4261 agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag tgttctttac
     4321 ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt gcttcttttg
     4381 tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt tggataaatt
     4441 gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt cctgaacgca
     4501 aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca acccgacctt
     4561 cgattccttc tggtactaat ttatttgctt cattgacact accttggaag taacggtctt
     4621 ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg aatcgacgtc
     4681 cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata cttcctagca
     4741 tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat ttgatccctc
     4801 cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca taaatagctg
     4861 tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat cctggtccga
     4921 taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct tcagccgtcg
     4981 caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct tgaatttttc
     5041 ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct gcccctgctt
     5101 ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct gctgctacaa
     5161 gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc tcgatgtctt
     5221 tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat ttttcgattt
     5281 tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg gcagtcacta
     5341 agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca aaacgcatgt
     5401 cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg attggcacac
     5461 cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc tgtggcgtta
     5521 agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt acttcatccg
     5581 cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt cttgccatag
     5641 caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt ggaatattca
     5701 acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac acgtgacttt
     5761 ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac tttgtttccc
     5821 agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat aattgatttt
     5881 ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa taccaatata
     5941 aagcgtttac atctgtgtat attaaaagag attgtaaaaa agctgtcctg catcaagtaa
     6001 taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt ttattaccgc
     6061 agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc ttagtttcga
     6121 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata
     6181 ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca agacttttgt
     6241 cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt actaccccta
     6301 gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg gataaacttc
     6361 caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt gccccgataa
     6421 tgataactac tactggatct aatactggat tgataactgg tggcaatagt gttgtcgcag
     6481 aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc ttacccattc
     6541 ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat ttgtacatca
     6601 tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag ccataagctg
     6661 gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat agaccaagca
     6721 acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt acttctgctg
     6781 gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga cgggctgttc
     6841 ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct tttacttttt
     6901 cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg tactgttgat
     6961 ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct cgtaattgtt
     7021 ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt cgtcacaaaa
     7081 gacattctac catattctac agctagtttg atagcccttt tacacattaa agcggaataa
     7141 cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac gtccagcttc
     7201 ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag tttctgcacg
     7261 gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg ctttgattcc
     7321 ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag tagctaagcc
     7381 taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga ttcctaaagc
     7441 ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt ctgcacgagc
     7501 actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt cttgtacata
     7561 atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat aaagaattgg
     7621 ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag taaattcgat
     7681 cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta cagctagttc
     7741 tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat agcgtttgtt
     7801 gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat cttctagtgg
     7861 atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa caacgtgaca
     7921 aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt ctcctttact
     7981 tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa cagttttttt
     8041 cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta ccatccctac
     8101 gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt ttgtgatcgc
     8161 attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag tcaatgccat
     8221 aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt cttttcaaaa
     8281 tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt gatatctgcg
     8341 cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt catttcaacg
     8401 atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca tcaaattgaa
     8461 tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca atctcgatct
     8521 tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc atcaaacgat
     8581 cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt tttttctttt
     8641 cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg acagttaaat
     8701 tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt aaacctaaca
     8761 gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg acaagatccg
     8821 gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct aaacgactag
     8881 ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct tctgcttctt
     8941 ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc atcatcgctt
     9001 cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt ttggacgctc
     9061 gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg cgtacgatga
     9121 tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa gatacttctt
     9181 caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt agctggacaa
     9241 cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac agtgcatcga
     9301 ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc caatgcttgg
     9361 tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc atgactaggt
     9421 gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc gcggaagtat
     9481 ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata cattgtcagt
     9541 aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg aaccgtattc
     9601 agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat caaaatcgaa
     9661 tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca atcgatcaaa
     9721 atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga ttctcttgcc
     9781 atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg cacaatactc
     9841 aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt gactagaaca
     9901 tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt agcatttcct
     9961 tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca agcacctaga
    10021 ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga tattatttgt
    10081 gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc tttcttgggt
    10141 atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga tcagagacat
    10201 tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca atcgcttgtt
    10261 ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa tagccttctt
    10321 gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc gcacgagcag
    10381 tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct gcccggtcat
    10441 gatataagga agcatcgatt cctaataatc gggttaactc agataagaag gttattcgtt
    10501 tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt aaaggtatgc
    10561 taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta aagtctactt
    10621 caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt tcatccgtaa
    10681 tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa atagcgaatt
    10741 gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg cgaaattctt
    10801 ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga ttctctccct
    10861 actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa aaatttgaaa
    10921 agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat acacagaaga
    10981 gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt attattacgt
    11041 attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc attaggatac
    11101 ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt tctggttgga
    11161 ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg tagtcgatgt
    11221 tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc atggcttttt
    11281 tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt cgtgtcaatt
    11341 cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga cgtcttaaaa
    11401 agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct aaaaagtctt
    11461 ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct tgttcaactg
    11521 cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct acacggtgac
    11581 ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg tattcagcac
    11641 gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca tcgatcatga
    11701 caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga actttcaaag
    11761 ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg cttgtaccat
    11821 ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc ggacggagct
    11881 ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt tctacttttt
    11941 ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa gaagtagata
    12001 aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat aattgatgac
    12061 gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag cgagcgccta
    12121 ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga ataatttggt
    12181 gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta taggcactat
    12241 ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct tctgtaacag
    12301 aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac cgttcgatag
    12361 caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag ttattcggac
    12421 ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt ccggctgtaa
    12481 taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca tgacaaacac
    12541 catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt ggttcttttt
    12601 cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct cgcaatgctc
    12661 ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt ttatcgatat
    12721 tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct ttagctggtc
    12781 cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt gtcagtaata
    12841 atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg tgtcctgcgc
    12901 caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg ttcctccttt
    12961 tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg tacactgtcg
    13021 ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac gagatccaca
    13081 ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc tttttctaac
    13141 aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt ttctccgctg
    13201 aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt agctacggaa
    13261 attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc cagctgtgct
    13321 ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc ttccaataat
    13381 tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat caagtctgct
    13441 tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc ttctgtttcc
    13501 cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac atactcttcg
    13561 attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc tcttaacaaa
    13621 tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt acttaaccct
    13681 tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct ttgttggatc
    13741 atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc atattctgga
    13801 taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct caatgagcgg
    13861 atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc tttgtctgtt
    13921 tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg accattcaag
    13981 aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct taacaatagc
    14041 tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac tacatcttcc
    14101 cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac aagctgattg
    14161 ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc tgataatcgt
    14221 ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc acttaaacga
    14281 acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt atcaaattct
    14341 aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa aaagtgccca
    14401 ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg ttcttgatta
    14461 aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa acaattagat
    14521 gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga tgtctctttg
    14581 cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc tttcttaaaa
    14641 aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag gagaaaatat
    14701 gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat caacgatgtc
    14761 gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt ttttgttgtg
    14821 gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct tttcttaaca
    14881 acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt aatcaactgt
    14941 cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact ttttgcttat
    15001 ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac aaaaaaagcg
    15061 gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg actacttcat
    15121 cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa actttgtcaa
    15181 cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc tactactaaa
    15241 taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt gcttaatgca
    15301 gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg ctgccctgtc
    15361 cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg gcgcttttca
    15421 cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg atggatgaag
    15481 acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat cagtatgcct
    15541 tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc cattttgaca
    15601 agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa ttccgttaaa
    15661 gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat cgtttcgttg
    15721 actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt ctgttctact
    15781 gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt ctttcgaccc
    15841 attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg gtctttggcc
    15901 aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc ttcataaatc
    15961 ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt gctttcttaa
    16021 gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt cgaattttga
    16081 aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact acccagtata
    16141 atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc atcacgaaat
    16201 tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa tacgtacttg
    16261 caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg ttccctaagt
    16321 tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat tgccataacg
    16381 ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga ttgacgccat
    16441 tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc ggatctcttg
    16501 aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt ttacgcatgc
    16561 tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt gtaaataaaa
    16621 tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca aagtaataaa
    16681 caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt ccgcaaccgg
    16741 ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg tatttcttca
    16801 cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga ctaaattgga
    16861 gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa taaaatccaa
    16921 ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc gtttgacttc
    16981 attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc gaaaatgagg
    17041 ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag aatctcccgt
    17101 atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc tcattagttt
    17161 tctttccttt tctatctctc acttacgctc ataatcatac cataattacg attgtccttt
    17221 catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg cctaagctga
    17281 aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat ttttagtact
    17341 catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt ggtatgttct
    17401 tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga catactttga
    17461 tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat tgttaaaact
    17521 tatccacaag attatttttt tctatgggaa tcctatgtgg aaaaagttat gcacagggat
    17581 tatccacaga taataaaagg ctggttttac acagaaaaca cacgtgtaaa aaaagtaatc
    17641 cacaatcagg cgatttcctg tgtattccgt ctcctaacta ttggtatatc tgtatttttt
    17701 atccacagac gcaagaggaa atctattggt ttttacgagt tttccacctg tgcattagtg
    17761 gtgtggaaaa ctttttttga caactgtgtt tttctctttt ttctttttgt cgattgtgtg
    17821 gataattgta attaaaaaat ttttttcttc ggtttctgtg gaaaactatt gaaaaaaatg
    17881 ctaaattagt actagttatt cactttttaa tgaaggagga ggccttatat ggtatccctc
    17941 gatgctttat ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc tgctagctat
    18001 aatacatgga tcgaaacagc gaaccctcga acccttgatc aaaaccagct cgttgttgag
    18061 gttcctagca agattcataa agaatattgg gaaaaaaacc tggcgacaaa aatcgttgaa
    18121 gtcggttata tgttatcagg aaatgagatt atccctcgct ttattactgg cgaagaagca
    18181 gagcaagaag aagttataga agaaaaaaat ccaaaagttg tggcacctag tccgctaaaa
    18241 aaagccatgc tgaaccctaa atataccttt gatacgtttg tcattggtaa aggaaaccag
    18301 atggctcatg cagcagcgct cgttgtagca gaagatccag gttctattta taacccgctc
    18361 ttcttttatg gaggcgtggg acttggtaag actcacttga tgcatgcaat tggtcatcaa
    18421 atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac ttttgctaat
    18481 gatttcatca attcgatcca aaacaaaaca gcagaagagt tccgtcaaga atatcgaaat
    18541 gttgatttac tgctagtgga tgacattcaa ttttttgcag aaaaagaagc tacgcaagaa
    18601 gagtttttcc atactttcaa tgccttgtac aatgaaggga aacaaatcgt actaaccagt
    18661 gatcggttgc caaacgagat tccgaaactg caagaacgcc ttgtttctcg ctttgcatgg
    18721 gggctgtctg ttgatattac accgccggat cttgaaacaa gaacagcgat tttacgtaaa
    18781 aaagcggcgg ctgaacgtct agagatccca gatgacacac taagctacat cgcaggacaa
    18841 attgattcta acattcgtga attagaaggt gcgcttgttc gagtgcaagc ttttgctgcg
    18901 atgaatagtg aagatatttc gactagtcta gctgcagacg cactgaaaac gttaaaatca
    18961 ggaaaaggac atcctcaatt gtcaatcctg cagatccaag aagaagtggc aaagtattat
    19021 cacatccaat tgaaagatct caaaggaaag aaacgcgtca aatctattgt tgttcctaga
    19081 caaatcgcta tgtatttggc acgtgagctc acagataatt ctttaccgaa aatcggagct
    19141 gaattcggtg gcaaggatca tacaacggtt atccatgctc atgaaaaaat ccagcagctg
    19201 atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt tttactaaat
    19261 taatttgtcg attctatcaa ctgaaaatag catgtggata aaagaaaaaa acgttcaaaa
    19321 gttatacaca ctttttccac agctgttttt tcgtttctct cttacgtttt ttgagttttc
    19381 cacagaatca acatgcccta ttacttttat tattcttttt aactaataat aaataaacaa
    19441 acattcaaag gagatacatc atgaaagtta ctttaaaccg agctagcttt atgcaggaat
    19501 tgcaaactgt tcaacgagct atttcaagca aaaccacgat ccctattttg acaggtgtaa
    19561 aaatcacact gacacaagaa ggtttgactt tgacggggag caacgctgat atatcaattg
    19621 aaactttttt gtctgttgaa aacgaaaaag caaatatgca aatcgaatct actggttcca
    19681 ttgttttaca agcacgtttc tttagcgaaa tcattcggag acttcctgaa gaaacattta
    19741 ctttagaagt tttagaaaat aaacaagtag cgatcacttc tggaaaagcg aattttatcg
    19801 taaatggatt agatgcagat aactatcctc atcttcctgt tgtcgaaagc cataaccaga
    19861 tgaaattacc tgtacacgta ttgactaaac taatcaacga aacagttttt gctgtctcac
    19921 aacatgagag tcgtccaatc ttgacaggtg tgcatttcat tttatctgat aattctttat
    19981 tagctgtagc tactgattct caccgtctaa gtcaacgcgt gattccagta gaacaagcgg
    20041 ctgatcattt tgatattgtt attcctggaa aaagtttgat cgaattatct cgctcattaa
    20101 caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg ttcaaaacag
    20161 aaacgatgta tttctattcc cgtttgctag aaggaaacta tccagatacc aatcgtttga
    20221 ttccttcaag ttttaataca gaggttgaat tttctgttcc tagcttttta gcggcgatcg
    20281 aacgggcttc tttactttct catgaaggcc gtaacaacat cgttcgttta tctattcgtc
    20341 cagatgcggt tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa gaaagcttga
    20401 gttatacagc aagcagtggc gatccattag atatttcttt caatccggat tatatgaaag
    20461 cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa atttatctct gctattcgtc
    20521 catttacatt ggaaccaacg gaagatggcg ttcaatttat tcagctgatt actcctgtac
    20581 gtacaaatta acaatgattt tcaatgttct tgaaaattta attgaaaaga aatagaaaaa
    20641 ataaaatttt aaacagaagg ccctaaaatc tcttttaagg ctttctgttt atttttgttt
    20701 aaatcacctt ttttagatta aatcgtctta tatggaaaaa tatatggata aatttgtttt
    20761 attgcgaaaa aaaaggtata atagagtata cttttaataa tgaaaatgag ggatgggcat
    20821 tgaaacaaca agtcattcta aatacggaat tcatgacgtt gggccaaatg ttgaaagaag
    20881 tcacagttat cggtagtggc ggacaagcaa aatggtattt ggcagaaaac actgtgttag
    20941 tcgatggcga accagaaaat cgtcgtggac gcaaacttta tcccggcatg atggttgaag
    21001 ttccagaagt aggaactttt tttatggtaa aaaaaggaaa cgacgataat gaggctgaat
    21061 aagctgtatt taaaaaatta tcgaaattac gaagagctga acatcgaatt ttccaagaac
    21121 ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga aagtatctac
    21181 gtattagcta tgacgcgaag tcatcggaca agtagtgagc aggaattgat tggctggact
    21241 gacgatcagg cgatgattca aggagagatc acaaaaggtt catccactct tccattagaa
    21301 atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca gaaaaagtta
    21361 agtacttacg tgggacaaat gaatgtcatt ttatttgcac cagaagatct ttctttagta
    21421 aaaggcagcc cgcagaccag gcggaagttt ttagatatgg aattaggcca gatcaatcca
    21481 atctatcttt atgatcttgt ccaataccaa gcaattttga aacaaagaaa ccagtacttg
    21541 aagcaattga atgaaaaaaa acaaactgat ttgctttatt tggatatttt gtccgaacaa
    21601 ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa gcgattggag
    21661 ttttgggcta attctttgca tcagcaaatc acccatcaaa aagagcagct ggaaatcgag
    21721 tatctcaccg cagtcgattc tttagaaaca catacgcaag agcaaatcca agaacaattt
    21781 ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac gacagttggt
    21841 cctcatcgtg acgatctttc cttttttatt aatcagaaaa atgtgcagac ttatggatcg
    21901 caaggacagc aaagaacaac tgcactaagt gtcaaattag cagaaatcga tttgattaaa
    21961 gaagagacag gggaatatcc aatccttttg ttagatgatg tgatgagcga gctggatgat
    22021 aatcgtcaac tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt cttaacaaca
    22081 acgacacttg accatgtaaa aaataaaatg actgttgaac ctgagatttt ttatgttcaa
    22141 caagggaaac taaaaggaga agaaactgaa tgacagaaga aagaagttta gtagaacgcg
    22201 ctaaagagta tgatgccagt cagattcaag tacttgaagg acttgaagcc gttcgtaaac
    22261 gacctggcat gtatatcggt tcgactagtt ctgagggtct gcatcacctt gtttgggaaa
    22321 ttgtcgataa ttcgatcgat gaagtattag caggttttgc aacgaaaatc catgtgatta
    22381 ttgaaaaaga caacagtatc accgttattg acgacggtcg ggggatacct gtagatatcc
    22441 aggcaaaaac tggacgtcct gcagttgaaa cggtctttac tgttttacat gctggaggaa
    22501 aattcggcgg tggtgggtac aaagtttctg gtggacttca cggagtagga tcttctgttg
    22561 ttaacgcgct ttctacacag cttgatgtca aagtatacaa agaaggaaat gtgtactatc
    22621 aagaatatcg tcgtggagct gtagtggacg acttaaagat tatcgaacaa acggatcgcc
    22681 atggaacaac ggttcacttt acgccagacc ctgaaatttt tacagagaca actgagtttg
    22741 atttttcaaa attggctacc cggatccgtg aacttgcttt cttgaaccgc gggatgcgta
    22801 tatcgattga agacaaacga gaagaagaac cagtcatcaa tgaataccat tatgacggcg
    22861 ggattaaaag ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt ccagaacctg
    22921 tatatttgga aggtgaacag caagatatcg cagttgaagt ctctatgcaa tatacagacg
    22981 gctaccattc caatatcatg agttttgcca acaatatcca tacgtatgaa ggcggaacac
    23041 atgaatcagg attcaagact gctttaactc gtgtgataaa tgattatgcc cgaaaacaaa
    23101 agctgatgaa agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa ggattgacag
    23161 ctgttatttc tatcaagcat cctgaccctc aattcgaagg acaaacaaaa acaaaattgg
    23221 gcaattccga agtacggaca gtcactgatc ggttatttag cgaacatttc atgaaattct
    23281 tgctggaaaa cccaagtgtg ggccgtcaaa ttgttgaaaa aggattacta gcttccagag
    23341 ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct ttagagatta
    23401 gtaatctgcc aggaaaatta gcggactgct ctagcaacga tcctgaaaaa tgcgaattgt
    23461 tcatcgtcga aggagattcg gccggcggat cagctaagca aggtcgtaat cgagagtttc
    23521 aagccatctt gcctattcgt gggaaaatct tgaatgttga aaaagcaagc atggacaaga
    23581 tcttggcaaa tgaagagatc cggtctttat ttactgctat gggtacaggg tttggagaag
    23641 attttgatgt atcgaaagct cgctaccaca aattagttat catgactgat gccgatgtcg
    23701 acggcgcgca tatccgtaca ttgctgctga ctctttttta tcgttacatg cgtcctattg
    23761 ttgaagctgg atacgtctat attgctcagc cacctttata cggggtaaaa caagggaaga
    23821 atattacgta tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa gtcgttgctt
    23881 cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa aggacttgga gaaatggatg
    23941 atcatcaact atgggaaaca actatggacc caagcaatcg gatgatggcc cgcgtcagtg
    24001 tggacgatgc tattgcagca gatcagatat ttgaaatgtt gatgggggat cgagtagaac
    24061 cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa gaacttagat atttaatcat
    24121 gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca tccatgatgt caatctaacc
    24181 agcgaaatga aagactcctt catcgattat gcgatgagtg ttattgtcgc acgtgcactt
    24241 cctgatgtca gagatggtct gaaacccgtt catcgccgta ttttatacgg catgaacgaa
    24301 ttaggtgtaa caccggataa agcccataaa aaatctgcca gaatcgtcgg agatgtcatg
    24361 ggtaaatacc atcctcatgg agactatgcg atctatgagt caatggtacg gatggcacag
    24421 ccattcagct accgttatat gctagtggat ggtcatggaa actttggttc tgttgacggt
    24481 gacggtgccg ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc tacagaaatg
    24541 ctgcgtgata tcaataagaa cactgttgat ttccaaagca actatgatga tacagaaaaa
    24601 gaaccagttg tactacctgc tcgcttcccg aatcttttag tgaacggaac aacggggatc
    24661 gccgtaggta tggccacgaa tatcccacct cataacttgt ctgaggtagt agcggctatc
    24721 gatttgctga tggataatcc tgatgtgacg accaatgaat tgatggaagt attacctgga
    24781 ccagattttc caacaggtgg tttggttatg ggaaaatcag gaatccgaag agcgtatgaa
    24841 acaggtaagg gttctattac cgtacgtgca aaagttgaga ttactgagat gccaaatggt
    24901 aaagaacgga tcatcgtgac agaattgcct tatatggtca ataaagcaaa attgatcgaa
    24961 cggatttctg aactgcacag agacaagcgt atcgaaggga tcacagattt gcgggacgaa
    25021 tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag cgcatctgtt
    25081 atcttgaata acttatataa attgacatcc ttgcaaaatt catttggatt caatatgttg
    25141 gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac aaattcttga aaactatatc
    25201 gaacatcagc gcgaagtcat cacaagacgg acagtctttg aaaaagaaaa agcagaagca
    25261 agggcacaca ttttagaagg tttgcgtatt gctttggatc atatcgatga aatcattgcg
    25321 attatccgcg ggtctaaatc agatgacgaa gcaaaagcga cgatgatcga acggttcgat
    25381 ttatccgatc gccagtctca agctatttta gatatgcgtt tacgccgatt gaccggtttg
    25441 gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa aactgattga agatttaact
    25501 gacattctgg caagaccaga acgtgttaca gaaatcatca agaatgaatt agcagacttg
    25561 aatcaacgat ttggtgataa acgacgcaca gaattactag tcggtgaggt cttaagtctg
    25621 gaagatgaag atttaatcga agaagaagaa atcgttatta cattgacaaa caatggttac
    25681 atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa ggggtggacg tggtgtccaa
    25741 ggaatgggta tccacaatga cgacttcgtc aaaaatctgg tttcttgttc tacccatgat
    25801 acattgttat tctttacgaa taacggaaaa gtgtatcggg caaaaggata cgaaattcca
    25861 gaatacgggc gcacagccaa aggaatacca attatcaatt tactaggtat cgattcgagt
    25921 gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag aagaaggcca ctatctcttc
    25981 tttacgacgc gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc taacatccga
    26041 agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa tgtgctactg
    26101 acagatggtc aatctaatat cattattgga acacataacg gctactctgt cactttcaaa
    26161 gaagaagcag tccgcgatat gggacgtaca gcttccgggg ttagaggaat acgcctgcgt
    26221 gaggaagatt atgtcgttgg ggcagcctta atgaaggaag atcaagaagt attagtgatc
    26281 acagaaaaag gttacggaaa acggacaaaa gtatcggaat atccagtgaa aggccgcgga
    26341 ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg gtccactagc tggattgacc
    26401 actgtttctg gagatgaaga catcatgctg attacagata aaggtgtcat tatccgtttc
    26461 aacgtatcga ctgtttctca aactggacgt tcaacattag gcgtacgttt gatgaagatg
    26521 gaagctgata cgaaagttgt cacgatggca gcagtcgaac cagaagctgc agaacaaaca
    26581 gttgaagaac aaggatcaga agaatagcca tcaagactaa ttgtgtaagc tagcaagaat
    26641 gaccgattaa aaaatctggt tattcctgct ggcttttttg attagttgaa tttctcttaa
    26701 aatattcgaa actgttcatt ctgtattgct tttgaccaaa atagagtgta taatcagagc
    26761 ttgtgagtag tctaattaat tagataactc tccttgctct taggaaccta agagccgaaa
    26821 gtccacaggg aggtgaatat caatggaaaa cacgaaatat gaaatcatgt acattattcg
    26881 tccaaacatt gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg caatcttgaa
    26941 agataacgga gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt tcgcttatga
    27001 aatgaacggt taccgtgaag gtatctatca catcgttaaa gtatcttctc catcatcagc
    27061 aaatgctgtc aatgaatttg accgtcttgc taaaatcaac gacgatatca ttcgtcacat
    27121 gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa cgatgagagg
    27181 agatgactat tttgattaat aatgttgtat tagttggacg tctgacgaaa gatccagatt
    27241 tgcgttacac agcaagtggt acggcagtcg caacatttac tttggctgtg aatcgtaatt
    27301 ttacaaacca aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt tggcgcaaat
    27361 cagcagaaac tttagcaaac tatgctcgta aaggaacact tttaggtgtt actggacgta
    27421 tccaaactcg ttcttatgat aatcagcaag gacaacgtgt atacgtgaca gaagtagtag
    27481 ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa caaagcggcg
    27541 gattccaaag ttctggtcaa tcagctggcg gatttggcgg aaacaataac tctaatcaaa
    27601 catctcaatc atccaacggt atgcctgatt tcgaccgtga tacttctgac ccattcggct
    27661 caagttcaac gatcgacatt tcagacgatg atttaccatt ctaaacgaat ttgataggag
    27721 ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt agactacatt
    27781 gctgctaacc atattgaata tatcgattat aaagatatcg aattactaaa aagatttatt
    27841 tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta caggcgctaa aaaccaacgt
    27901 aaaattactg ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt cgttagcgaa
    27961 gactaataaa tcctaggaat tgaatgactg atccttttga tcagctattc aattcctttt
    28021 tttctgtaaa aataacctct attcgtttag tcgctcgaat gacgaaagtt atggtaaaat
    28081 caaattagtc atatatggga ggaaaattat ggaaaataaa aacaatcatt tatccccgaa
    28141 acctctcttc cttatcctgt ttatattaat cgaagtattg gtagtcttaa ttttaccatt
    28201 tcgttttgtt agcgcttcgg tcattctatt gatgaattta tggggtgttc gctattggtt
    28261 gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa atccagcagg cttcgactgt
    28321 agctgaaacc aatctagctt atgtctcaga agtcatgcct gtaggagtga ttgcttatca
    28381 gccatcaagc caaaaaatcg aatggctcaa tccattagca ttgaaaatca aagatcaaag
    28441 tggattaaca gaggaagaat tgctagatac tttttttact gcaaaagaaa aaaagaaaaa
    28501 ccaagtctat ctagcagata aaacatttca gtttgatatg gatacagtaa agggtgttgt
    28561 ttattttaat gatattaccc aaaaccagtt gttaaaactc catcagacga acagtcagcc
    28621 ggtgattggt attttatcta ttgataacta tgcagatgcc attgataaat tggatgacaa
    28681 agagatttct tatttgaact ctttcgtcac tacactagtt tctgactgga tgaatgaata
    28741 ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac agatgatcga
    28801 tctgaaaaag atgatggata aaaaatttga tttgctggat cgctttcgag cagaagctga
    28861 aaaacagagc tcgcctatca cccttagtat ggggattgct tatggctcaa gaaccctcga
    28921 tgaaatcggt ggagaagctc agaataattt agatgttgct ttagtccgtg gaggcgacca
    28981 agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt tattatgggg ggaaatctgc
    29041 taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt tgcgtcggat
    29101 ttttgatgaa acaggcgatg tgtttatcat ggggcacaag tttccggata tggatgcttt
    29161 aggctctgct tttggtgtca gttgtctggc acgcttcaac cagaaaaaag cttacatcgt
    29221 tatcaacgag aacgagatca ttccagatgt agaacggtgt cttgaagaaa tccataagca
    29281 tcctgaatta gaaatgcaac taatctctcc agaacgtgcc atagagctga aaaaagaaga
    29341 ggacctcctc gtgatggtgg attaccataa accttctcta tcgatctctc agggcctata
    29401 tgagcaattt gataagattg tgatcattga tcaccatcgt cgtggggaag agtttccaag
    29461 taaacctctg ctgacttata tcgaatcttc agcttcttca gcttcagagc tagtttctga
    29521 gctgatccaa taccaaagca gcaaaacaaa tcgcttagat cgttttgagg ccacattatt
    29581 gcttgcaggg atggttgtag atacaaatag tttcaatgtc cgtacaactg cacgaacttt
    29641 tgacgttgcc agctatttaa ggtcttgtgg agcagacgca tccttagtac aatatttatt
    29701 gagctcggat ttagcgacat acttggagat cagcaatttg attgctggaa gcgactatgt
    29761 gacttctgat attgttgtgg cggcagggac agacgaaaaa gagtataata gtgttatagc
    29821 agcaaaaaca gcagatacat tgctttcgat gatcaatatc aatgcagctt tcgtcattac
    29881 aaagcggaca gacggcttga ttgggatcag tgccagaagt accggcacga tcaacgtcca
    29941 aatcatcatg gaatccttag gtggcggagg acacttcaca aacgcagcta ctcaactgga
    30001 aaatacgaca gttgaggaaa caaaacagaa gctatttgct gcaatcaaag aaaatatgaa
    30061 tcaaatatat gataaggagt aatccaaatg aaagtaattt ttttacaaga tgtaaaagga
    30121 aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt atgcacaaaa ttatctaatc
    30181 aaaaataact tggcaaaaga agcaacgaaa agcagtatca gtcagttagc aggacaaaag
    30241 aaagcacaag aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt aaaagaattc
    30301 ttggaaaaag aagatactgt agtcgaattg aaggcgaaag caggggaaga cagccgtttg
    30361 tttggttcga tcccttcaaa acaaattgct gaagggttaa acaaacaata caaaatcaaa
    30421 ttagataaac gaaaaatcga attagaaaat ccgatccgtt cactaggcta cacaaacgta
    30481 cctgttaagc ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt acaagaataa
    30541 caaatcttga tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa gacaaaccga
    30601 aatttatctg agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg atttcagatt
    30661 gatttcggtt tcttttttgg gaacagcaaa atagcataaa gaaaacgagt ataataaaag
    30721 agaactattt ttttattaca ctttcggtac gaatgatgac ttttctctca ttttcttgta
    30781 aaatgaagca attgaagaca aggagcgata cgtatgaatg aagtgtggca ggatcgaatc
    30841 ccaccgcaaa atattgaagc tgaacaagcg gttttaggct ctgtttttct tgacgcagaa
    30901 acgatcattg atgccatgga gtacatagag ccaaaagact tttatcgtcg tggccatcaa
    30961 attatttttg agacaatgat cgagttaaat gatcgtaatg aagcgattga cgtggttact
    31021 gtcaaagacc ggctggaaca agccaatttg ttagaagatg ccggtggttt aagttatctc
    31081 tcagacttgg cattagcagt tccgacagca gcaaatatcg tctactatgc gaaaatcgtt
    31141 gaagaaaaat cattgctgcg tacattgatc cagacagcaa caggaatcgt tacgaaagga
    31201 ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag tatcttggag
    31261 gtttcggaaa aaagaaatcg aagcggcttt ttatctattg ctgaagtact gaactccacg
    31321 attgaaaata tcgatcgact agctcaaaac aatgaagaaa tcacaggtct accaactggc
    31381 tatcaaggtc tggataaaat gacagcaggt ttccagcccg aagaactaat cattcttgct
    31441 gcacgtccag cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa tgtaggaaca
    31501 aaaacagatc gttctgttgc gatatttagt ttggaaatgg gggcagaatc tcttgtaaat
    31561 cggatgcttt gtgcagaagg ttcgattgag gccagccatt tacgtacagg tcagctttcg
    31621 gaagaagaat ggtcgaatct gatcgttgcg atggggagtc tttctcgagc aaatattttt
    31681 atcgatgata caccagggat caaaatctct gaaatccgtg cgaaatgtcg gaaactagcg
    31741 caagaaaaag gcaatctagg cttgatattg atcgattatt tgcagttgat tgaaggtaac
    31801 ggtaaagaga accgtcaaca agaagtatca gatatctctc gccaactgaa aaaactggcg
    31861 aaagagctga aagtacctgt cattgctctt tcacagttat ctcgtggagt agaacagcga
    31921 caagacaaac gaccagtgct aagtgatatt cgtgaatccg gatcaattga acaagatgcg
    31981 gacatcgtag ccttcttgta tcgagatgat tattatgaaa gagaaggcgg agatgacgat
    32041 gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa gaaccgaagc
    32101 ggtgcacgag gaacagttga actactgttt atcaaagaat acaataagtt ttcgtcgctt
    32161 tcgccaagag aagaatttta aagaagaggt tgtgagcctt gagcaatccc gagagctctc
    32221 gtaagaagat gcaaggagta accgcaggaa cgactagagt acttaattta aacagatctc
    32281 caaagggatt gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac ggtgttcaat
    32341 cagctgaccc ttggtattag ctcagcaaac gtcaaaatat gaggaactat tttgacgttt
    32401 gtttctctaa tactcaggtc aaaacgctga tcttacgacc tcttttcctt aaacggtgtt
    32461 ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa acatgagaag cagttttttg
    32521 aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctttc ttaaacggtg
    32581 ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga gaagctattt
    32641 ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc tgattcacgg
    32701 tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt tgagaagcaa
    32761 ttttcgtaca ttccttctta tttcttgaag ctgaccactt ctgttacaac ctctttttta
    32821 ataaccattc taaaaagata aagttcgttt tttaagcgtt tttataaaat aaaaataaaa
    32881 atcattcgat tttatt
//
LOCUS       DANKAZ010000023        32618 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000023 DANKAZ010000000
VERSION     DANKAZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(99..1229)
                     /locus_tag="PWY67_001016"
     CDS             complement(99..1229)
                     /locus_tag="PWY67_001016"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL2057317.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="PWY67_001017"
     CDS             complement(1877..3079)
                     /locus_tag="PWY67_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2057318.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="PWY67_001018"
     CDS             complement(3139..3342)
                     /locus_tag="PWY67_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL2057319.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="PWY67_001019"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="PWY67_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="PWY67_001020"
     CDS             4879..5691
                     /locus_tag="PWY67_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2057320.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            5846..7185
                     /locus_tag="PWY67_001021"
     CDS             join(5846..6287,6287..7185)
                     /locus_tag="PWY67_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL2057321.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            complement(7210..7467)
                     /locus_tag="PWY67_001022"
                     /pseudo
     CDS             complement(7210..7467)
                     /locus_tag="PWY67_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7483..8133)
                     /locus_tag="PWY67_001023"
     CDS             complement(7483..8133)
                     /locus_tag="PWY67_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057322.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(8144..9316)
                     /locus_tag="PWY67_001024"
     CDS             complement(8144..9316)
                     /locus_tag="PWY67_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL2057323.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(9335..10207)
                     /locus_tag="PWY67_001025"
     CDS             complement(9335..10207)
                     /locus_tag="PWY67_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2057324.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(10229..11149)
                     /locus_tag="PWY67_001026"
     CDS             complement(10229..11149)
                     /locus_tag="PWY67_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057325.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(11149..11418)
                     /locus_tag="PWY67_001027"
     CDS             complement(11149..11418)
                     /locus_tag="PWY67_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057326.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(11415..11795)
                     /locus_tag="PWY67_001028"
     CDS             complement(11415..11795)
                     /locus_tag="PWY67_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL2057327.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(12262..13725)
                     /locus_tag="PWY67_001029"
     CDS             complement(12262..13725)
                     /locus_tag="PWY67_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2057328.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(13789..16806)
                     /locus_tag="PWY67_001030"
     CDS             complement(13789..16806)
                     /locus_tag="PWY67_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2057329.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(16957..17796)
                     /locus_tag="PWY67_001031"
     CDS             complement(16957..17796)
                     /locus_tag="PWY67_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2057330.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            17941..18186
                     /locus_tag="PWY67_001032"
     CDS             17941..18186
                     /locus_tag="PWY67_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057331.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            18249..18671
                     /locus_tag="PWY67_001033"
     CDS             18249..18671
                     /locus_tag="PWY67_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057332.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(18765..19082)
                     /locus_tag="PWY67_001034"
     CDS             complement(18765..19082)
                     /locus_tag="PWY67_001034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057333.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(19109..19315)
                     /locus_tag="PWY67_001035"
     CDS             complement(19109..19315)
                     /locus_tag="PWY67_001035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057334.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(19296..19478)
                     /locus_tag="PWY67_001036"
     CDS             complement(19296..19478)
                     /locus_tag="PWY67_001036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057335.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(19492..19842)
                     /locus_tag="PWY67_001037"
     CDS             complement(19492..19842)
                     /locus_tag="PWY67_001037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057336.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(19868..20170)
                     /locus_tag="PWY67_001038"
     CDS             complement(19868..20170)
                     /locus_tag="PWY67_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057337.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(20173..20406)
                     /locus_tag="PWY67_001039"
     CDS             complement(20173..20406)
                     /locus_tag="PWY67_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057338.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(21392..21714)
                     /locus_tag="PWY67_001040"
                     /pseudo
     CDS             complement(21392..21714)
                     /locus_tag="PWY67_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21837..22952)
                     /locus_tag="PWY67_001041"
     CDS             complement(21837..22952)
                     /locus_tag="PWY67_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2057339.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(22958..23356)
                     /locus_tag="PWY67_001042"
     CDS             complement(22958..23356)
                     /locus_tag="PWY67_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2057340.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(24385..24771)
                     /locus_tag="PWY67_001043"
     CDS             complement(24385..24771)
                     /locus_tag="PWY67_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL2057341.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(24768..25289)
                     /locus_tag="PWY67_001044"
     CDS             complement(24768..25289)
                     /locus_tag="PWY67_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2057342.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(25291..26325)
                     /gene="sppA"
                     /locus_tag="PWY67_001045"
     CDS             complement(25291..26325)
                     /gene="sppA"
                     /locus_tag="PWY67_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2057343.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(26358..27773)
                     /locus_tag="PWY67_001046"
     CDS             complement(26358..27773)
                     /locus_tag="PWY67_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDL2057344.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(27742..29895)
                     /locus_tag="PWY67_001047"
     CDS             complement(27742..29895)
                     /locus_tag="PWY67_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HDL2057345.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            30153..30389
                     /locus_tag="PWY67_001048"
     CDS             30153..30389
                     /locus_tag="PWY67_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HDL2057346.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            30408..30623
                     /locus_tag="PWY67_001049"
     CDS             30408..30623
                     /locus_tag="PWY67_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HDL2057347.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(31326..32078)
                     /locus_tag="PWY67_001050"
     CDS             complement(31326..32078)
                     /locus_tag="PWY67_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2057348.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(32093..>32618)
                     /locus_tag="PWY67_001051"
     CDS             complement(32093..>32618)
                     /locus_tag="PWY67_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2057349.1"
                     /translation="NHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIA
                     KLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIY
                     RDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQ
                     NDPYQFTTQVILLF"
BASE COUNT         9552 a         6403 c         5025 g        11638 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gatgatgtgc accccaaaag ttagactaga
     5821 aatctaatta aaaggggtgc tttttttgcg gaaatataca tttgttttta agaaaaaagt
     5881 agtttcagac tatttaaata acgaaggcgg ctacaaatat cttgcacata aatatcaaat
     5941 aaatcgtaca ctggttagac attgggtaag gatttataac tatcatggtt gggaaggctt
     6001 ggttggaggt ggcaaaagct acactacaaa atttaaactt gatgttatag aatatatgga
     6061 aacaaatggt ctttctatcc aagaaactgc taaaaaattt aatatcggtt caaatagaac
     6121 tctaagtaaa tggatagagc aatatgaaga aggcggtgct tcttcacttg agagccaaaa
     6181 aaggggcaga aaaattagta tgaattccaa gctaaacatt cctaaaaaac ttaaagatga
     6241 gtctcttgaa gaagaagtta ttcgtttaag agcagagaac gcatatttaa aaaagttaga
     6301 aaccttgatt caagaacaag atttgagcaa gaagaaatca aggttaaaac aatccttcag
     6361 ctcaaaaaag aatttaaatt gaatctatta ctttcaattg cacaattagc gaagtcaact
     6421 tattattact gggtaaaaaa attagataag ccagataaat atagcaagat aaagcaagaa
     6481 attacagcga ttgtaaaaga atcaagaaac tcttatggtt atcgtagagt cactttagcg
     6541 ttaaagatga aaggatatac aatcaatcat aaaacagtta gaaaattaat gtcccaaatg
     6601 ggacttacct gtcaaatccg aataaagcga tataaatctt ataaaggaac agtaggaaaa
     6661 attgccaaga atgtgttaaa acgaaatttt tcagtagata cacccaataa aaaatgggtg
     6721 acagacgtca ctgaattcaa gataaaagga agaaaaatct atctatctcc tatacttgat
     6781 ttatttaacg gagaaataat tagttatagt atatcaacta gtccaacata taaactgatt
     6841 gaagaaatgc ttcaacaagc aattaaaaaa aagggaactg agggttcact gattctacat
     6901 tcagatcaag gatggcagta tcagatgcca caatatcaaa aaaaattaaa agagaataac
     6961 attattcaaa gcatgtctag aaaaggaaat tgcttagata attctgtaat agaaaatttt
     7021 tttggtgtgc ttaaatcaga gtttttttat cgagaaaaat tccgatcaat tgagatattt
     7081 caaagtaaat taaatgagta tattaggtgg tataataaca aaaggataaa actaaagtta
     7141 aatggactat ctccagtaga atatcggaaa cagtccatta aatagttcaa cttttggggt
     7201 tcagttcacc aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa
     7261 agcatgacaa atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat
     7321 ccgccacaaa acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc
     7381 ataagaaaaa gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac
     7441 ctctcttatt gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg
     7501 ttgattaaag ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa
     7561 ggttacgtca aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata
     7621 cgacaccaca acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca
     7681 tttctctaat tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata
     7741 ttgcttcatc agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga
     7801 atcaggctca taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg
     7861 aatgtttcct ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt
     7921 cgtgacctcg ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg
     7981 tcctttcttc tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt
     8041 tagctgtagc ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc
     8101 gcctacacaa acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc
     8161 gagcaaaaca aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt
     8221 cacctacacc gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc
     8281 aatcttccgc taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa
     8341 tgataatatc gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc
     8401 ctcttgcgtc gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc
     8461 ctgctttcgt taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg
     8521 gattttcgac actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca
     8581 gactttttgc tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta
     8641 gacaataatc ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag
     8701 cgacgggatc actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac
     8761 gattccccat cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt
     8821 cctctacgct ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat
     8881 gaatcaaaaa ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa
     8941 aatttttcaa agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg
     9001 gatcgtttgg tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta
     9061 acatacccgc tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa
     9121 tttgaagccc gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt
     9181 cggttggact acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa
     9241 acagaaaacc aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa
     9301 gaagtttttt cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc
     9361 ttttgcgcca aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa
     9421 cgagagacac ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca
     9481 ccgcaatatt tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc
     9541 ctgattcttt ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt
     9601 caattggtgt acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt
     9661 gaacattttg tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt
     9721 cctgtactgg aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag
     9781 tcgtatttcc ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac
     9841 catttcctgg gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat
     9901 ctggattttc tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg
     9961 ttggtggaat ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt
    10021 ctcctccatc tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg
    10081 gtggaataat ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg
    10141 cactggttcc taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt
    10201 ttttcatgtg tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg
    10261 gaagcgtgga aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg
    10321 tttcttgtgt ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc
    10381 cttctttctt catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt
    10441 gtttcgcaac tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca
    10501 tttcacccga ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt
    10561 ttgtcgcaag ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta
    10621 gttgttcaat ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga
    10681 cgataaatcc aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc
    10741 tttgtgtaaa cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg
    10801 gtcgacgttc gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta
    10861 aggtttgaat aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt
    10921 caatcaatga aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg
    10981 gaatttcatg gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca
    11041 atagctcttc attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat
    11101 ctgttagtaa agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac
    11161 ttcttccacc acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac
    11221 ccgatattct tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt
    11281 atattcgttg cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc
    11341 aatctttgga aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat
    11401 tcctgttact ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt
    11461 gtcggtaagc aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa
    11521 aaaataaatg cccaatgaaa acctgcgtct cgtaaacgtc gaacgctgag agaaagagac
    11581 gggacaagaa ctacgaaaca atacacaatc aatacgccta tcaaacaacc aattattgtt
    11641 tcagaagcat tcaacgctcc tgctagtcca atcgctaagc ctaggccaaa cccaatgagg
    11701 gcattcatta acgcaacgag ccaatactct tttcttgtgg ctttcccttt gtaattaaag
    11761 ctattctgcc aaaatttttt ataatttgtg atcatatctt tgtcctttct aatgtttatt
    11821 tttgattcta gtttcttctg ttttactaac aatgaaactt gccataaaaa aggtggccaa
    11881 tacaccacca gctagaaatc cacttacaaa taacaaaatt ttttcctcct ttctaaaaaa
    11941 tttcgccata aaaaggcaaa gtatgaacac gataaatacc atgtacatac tttgcctttt
    12001 tatgcaatat atgttaactc tccaagcaat tactgatttt taaattcttt ataaataaaa
    12061 taaaaatcgt ttgagtaaag aaataagtca atgaagaccc aaacgataaa aaatattcct
    12121 aatactatat ttaataaact acgttctaga tagatacgag atttcaaatt aaaaaacgaa
    12181 aatgagagat acaaatatat caaatattta aaaagtaacc acattttcta taccactcct
    12241 tatcagaaac agccttattc tttagaataa gactgtttct gggttgatta atttgtcttt
    12301 tgttaaacta ccatctaagg caagttctaa atgtaaatgt ggtcctgtac tatttcctgt
    12361 tgaacccaca taaccaataa tttgtccttg ttcaactgtt tgtcctacgt tcacgcaata
    12421 ttcctgttgg tgcgcataaa gagccacact tccgtcttca tgctcaattg tgacataatt
    12481 tcctgacaaa gtatgatacg aagcttctat gaccgttcct gccttgctcg catagattgg
    12541 ttctccttga ttggctgcca tgtctaatcc tctatgaaat tcttctcctc gccaaccgaa
    12601 ggaactagaa attgaataat tttttagagg taccatatag ccttctttgc ttagttgtgg
    12661 accagctact tctttatcat atttgattaa gtcataggtt tcgattaacc catttaattt
    12721 tttatcgtag ctagtgtctg ttgcgtatct tcctgttaaa aatttagtcg cttcctgata
    12781 ggtttttgcc ttacttttcc aaacaccatc ataaaaagag gtatttccaa ctaagccttc
    12841 ttttaacagt ttggcatagt cttttaagct ttcttcatag ctctcatatt gccgaaaagt
    12901 tgcttgggtc gtataaagtg tcccattgcc taaatcttct tgtgtcgcaa aagagacgcc
    12961 ctttccgtta tgtgtgcctt tgattccaaa tagattgtag tttggtgcct gagccaattg
    13021 actttgtccg cttgcggatt ctaaaatggc ttgcgcgatc ataacagaag cgtataaatc
    13081 attttcttgt ccaattttac gagcagattc accgatttta cggataaacg tttctacaga
    13141 cgcgtctttt tcaaaatgaa tagtcccttc gttatcttgt gtgctggaag ggactaattc
    13201 ttgtatcggt gcgcttggtg cttggtggta gttttttgtc tcttctgaac cgattgattg
    13261 tgcctgcggt acgtcattca taggtacagt cacactctgt tcaggtgcct tttgtgggcg
    13321 ttccgttttt gttggcggtt ccgcttctgt ttcggtactt ggtttttgag gagtctgtga
    13381 gctttcatca gaaggttgct catttggttc ttctgacggt tcctcctcag gttgttgatt
    13441 gtcttctggt ttgtctggtt gattcggtgt ttctggttgt tcttcagaac ctgttggtgg
    13501 agcgttcatt tctggattat ctggtgtatc tattgtatct tcagtcggtg tttctggttg
    13561 attttcttct ccagtggaat ttgttgtcgt ttcttcagtg atcgttgcag attgcgaatt
    13621 tgtcagttga ttgttgcctt gttcattagc caaaaccgct ggcgtatacc caaatacttg
    13681 acctactaat aacaaagcag ataaacattt actcgttttt ttcacaagat tgcctccatt
    13741 cttcaatcaa tcatctagag acagctgatt gctgtctcta gtcatacttt aattgctttg
    13801 tttcatttta cgacgaacca gaacagcccc cacaccaaca aaaagaaaca gtcccaatgc
    13861 aattaacaca tcgttcactg tttctcctgt ttttggtaag ctttgttttg gtgtgctcgt
    13921 cggcgtttgt ttgacagggg ttgtcggcgt ttctttttta atgccttttg ctgtcaatgt
    13981 ttgtttttca ttcttcaaat caaagtgacg tgcttcttcc tctttatggt ctttatcctt
    14041 atagacaata tgagttgcga ccaatttctc cccgtctttt aacgtgcctg ctttgtagtc
    14101 aaaggatact ttaaactctt cagacgcttt agaaacttta cgtgcagttt ctaccgtacc
    14161 aacgactgta tcttttccat ttttatcaat cttatggaat tgagtcaccc agtatttggt
    14221 ttcccctacc gtagattctg gaaattcttg cgatacatga tcgatcaact cttggtcttt
    14281 gtctggatca aatgtttggt cgccattcac tgtaataaag cgtgtttcga tttttggttc
    14341 tttcgcagtc actgtttgat ctttattggt taaatcaaaa tgacgagcga attcttcttc
    14401 ctcttctttg tcgttataga caatgtgcgt tgcgacaatc gtgtcaccgt ctgaaaccat
    14461 gtctgctttg tagttgaacg tcacatcaaa tgtttctttt tcatcggtca cttttcgtgt
    14521 ttcttttacc gtatctaata cagtgacttt tccatcggca gtaattttat ggaattgcgt
    14581 cacccaatat ttctctgtcc caaccgcaga tttagggaat acttgggaca ctttatccac
    14641 taattcttgg tcttttttcg ggttgaaatc ttttccacct gtcgttgtca caaacaaagt
    14701 agaaatttct ggttggtctt cttctggaac cacgatttct tcgtttttca cagtaattgg
    14761 ttgtacttct ccgtctttga ccgttactaa aatcgtttct gtttgtcctg acgcttgccc
    14821 ataatgctca gggaacacaa tttccgtaaa tttatacgta ccgtctggta attttagttt
    14881 ttctggttct tcttttgttt catacacaaa ggacacattt ttattatgtg cagtcatacc
    14941 ttcaatcagg aatttcgctc ctggtaattg tagatgattg attgaatcga ctttattgaa
    15001 gtctacttca cccgttttta acaagttgtc aatcacatct aattcaaacg taagcacagg
    15061 cgtcgtgtta tcgccatagg ccacagagaa cgtggtttgt tcttcagaag gttgacggta
    15121 gttgtctggc acattttttt caatcaattg gtatttgttt tgatcgccat tgtagaattt
    15181 tttgtttgcc ttccagtgtc cgtctttgtc agtttttact gaatccaaga cttctccttt
    15241 tttcacaagg actgtttctt tatctggcgc agtaatatct gttttggcga taatatcaaa
    15301 ttcgatattt tctgcaggaa cggtatcata aacaggtttg ccttgattcc aggattcttc
    15361 cgcattatag acttctttga ctttctttcc ttctggaatg aactgttgta agtcattttt
    15421 tgcagttaac gtcacttctt tgtttggttc aatcgtaatc gtatgagttt gtttgtctag
    15481 aatataccca gctggtgctt tgacttcttt gacatccaat ttattttgaa catattttcc
    15541 gtctttttca tagaatgcag ggatttcttt taaggcgatt tcccctttgg catccgttgt
    15601 atattgtttt gtttctcccg tatcatgacg tttgatttca tacgtcacat ttggaacttt
    15661 tttccctgtg acagcgtcta gtttggttag tttgaagtcg ccatttttta gaactttaat
    15721 agttagtcca gctctattag gatctggcac ataaagcttt cctagatttt gagcaacagg
    15781 atctacatag gcaaaacttg aacctacccc tctagaattt ggatccagtg aagaaacaga
    15841 aattcgtccg tcatttgagt tttgatttgc ttttataaac aaactatttc ctgattttct
    15901 tacttgaacg ttagctgtgt tttcaacaac cgacccatcc cattgagaaa acacaccttt
    15961 tgtatcctta acttctactt cttcccctac tttaaccgtc actgtttggc tatcaaaaga
    16021 aggatcttca agtaaattgt ttatttttct atctacagtc tgtttgaaag cttcataatt
    16081 agattgtcca atagttccat aattaacaaa tgaaccacct aaaacttccc aaatatatgt
    16141 ttgtgctaat acatagttat ccattgaacc actagaatag tagccaaaat aaccaattaa
    16201 agaaatcttt tttctaatag cacttgtcgt aattaaattt tcgagtggtg tcacatcata
    16261 ttgaataccc gcactcgtga ttgcgccttc ttgaatgcaa aaaacatctt ctccgtcaat
    16321 ttgccagcga accatgtaat catgagtata cattgttcct tcaggagtaa ttcgagtcat
    16381 attcacatct ttacgaccat tttcagtcaa tgttagattg gtagcacgtg tctgtgcatt
    16441 cttttccaag attgcttgcc attcttcaga ttcttgcgcc aatcgttctt gttcagaaaa
    16501 agaagtcgct ttttgaatat cttcaatggt tataaagtat ttctcataac cttcagggac
    16561 ctcttgagtc attacattgg gcggagaaac atcttctgtc gtgtcctgcg tagttgtctc
    16621 ttctgttgtt tcacttgtgg tttcagtact gatcgcttct tcactacttt ttgattcttc
    16681 agtcttcatt acttcttcag tagaatttga ttcatttatc gtttctgcaa atgccgttac
    16741 agatcctaac atgggattga gaaatgtaac gaaaattgcc aaccaagttg ccattctttt
    16801 aaacatattt ttttcgttcc tttcatttta tttttcagtc ttctaaagta atttcttatg
    16861 gacttcgaca ccgaaacatt tttatcctcc tcaaaacaaa aaaagactag aacttataat
    16921 tctagtcttc atcaaccact tacttttctt ttatttttag tagaaatcta cagtgtgttt
    16981 ttgtgacccg tcactgtaaa taataacatc caccataaaa cctttgttgt accactgcga
    17041 atttggatcg ctaacctctg cattagccca atcataggct tcatctacag tgttaaatac
    17101 ctttccacta ttaccaatct ctccaactac aattgttggc tcttcaggtg tcgttgtgcc
    17161 accatttccg ccttgatcgc ctccaccagg attgcttggt tcttcaggcg ttgttgttcc
    17221 tccacctgtg cttgggttag aaggagttgt ttctcctcca gtatttccac cattacctgg
    17281 ttgtgtaggt gtttgagtag tgccaccatt gttttctggt gtacttggcg ttgttgtacc
    17341 accagttgtt cctgtttgat ctccagctgg tgttgttggt gtcgttgtat ttggtgtcgt
    17401 ttgtgtattt gaagtcgttc cttgtcctac tggttgattc ggatcaactt tgtctttctc
    17461 ttgaatcact gtttgagcca cagtttcacc tgcgctgttt tgtacacttg ctacattgcc
    17521 attaaatact aatttatttc ctgcatagat taaatccaca tttgtaatct tattcatgtc
    17581 cgctaatttt tggacagtaa tatttgttgc ttgactgatt gcgcttaggg tatctcccca
    17641 gacaattgtg tattcattcc ctttaatttc tgctttgatt tgttccgccg aacgaggtgt
    17701 ccaaactttg ttttctgctg cttcagcatt cgttcccaca gcaagactaa ctcctccgac
    17761 tacgattcct agtgccaata ctttcaacat tttcattgtt tttcgcctcc gatttgaata
    17821 aatttattta caaaaacata gtaccaaact tatgtctatc agtcgatata ttttttgtag
    17881 atttttttat tttttgatat actaactttg gtactaaaat aactaaaagg aatgtcacac
    17941 atgcaactta ataatttaaa tgaaatacat gattgggctc aaaataattt acttaatcgt
    18001 aaacaagctg ccaaaatcac tggacaaaac tatgatgctt tttctagctc tttacgtaga
    18061 aataatatca aaccatttct agaaataggc gacgaacctg gtccttccat cattcgctta
    18121 tatctaagag aagatattga aaaatacgca aaacagctcg aagaaaaaag acagaaacgt
    18181 caatgaggcg ttttctgtct tggatttaca agtaaaacaa taataaacac ttttacaacg
    18241 gaggttctat gaataagaaa tacattgttt cagttggttc aatcgtactg attgtactcg
    18301 ctattattgc tttttctgta tccaacaaaa cagaaacaca gccacaaaat acggttcaat
    18361 cgactactac aagtcaatct agcgatcatt ccgtctatcc ttcactaaat agtttaaaag
    18421 aaacgcatgg acaagattta attgatcttg gttcttctca aattactact gtcttatttg
    18481 aaaagaaaaa tattgcgttt attactccta aagaaggaac tgaccttgaa ttttctatca
    18541 aagtaacaaa taccaaagaa aatttattct ccattcctga cattcaatat acccctaaca
    18601 agttaaagat tggggatacg tttggtttgg ctaaagtaaa tgaacaatac tttgcttact
    18661 cactgaaata agtttctaat agcaagagga aaaaccctct tgttattttt atcacataat
    18721 cattttataa aaacgcttcg aaaatatcaa catttttgaa gaaactattt ttctaatttc
    18781 tcaatttctt ccaagggatt ttcgctaatg acccatacag gcacagattg ctctcgaggc
    18841 tccccttctt ctaaccacca acctgcataa tgaacttctt cctcctcagc attaccatga
    18901 taccaaccaa caaaggattc atctacaacg aatgaaattt cttcttctgg atcaaataat
    18961 tcatttttcc atgcttcaat tgcttgttct ttggtgtact tttgtttgtt aaatacacca
    19021 caaatttcgt catctacata aaaaatttca aaatcaaaat tactcgttcc ttttactggc
    19081 atcttaactt ttcctccttt gactcacttc aatagatata ccactcatgt tcattccatt
    19141 gtttactagt taattggagt tttatcttag ggctttgtgc actctttcca aaaacttgag
    19201 cgtcgttttt ttcttctaac caaaaaaggt aatttcttac attcggtact aaatgttttt
    19261 cctgcatttc ttcatgaaaa acatataatt ctgcttcatt tttcaaccaa ttcattattc
    19321 attccctcat tcacttcttc ataccgccat tcagatagtg agatttcttt ttgtttctcc
    19381 acttgtccta taaaaatcgc tgtattcaac aagctgatca cacttaataa taaagcaatg
    19441 aaactaatgg ttacacttac tctttcacat tttgtcattt tgtttccttc tttactcgtt
    19501 tgttatttct tgtaataggt ctaataactc cttcaccgaa atttgcaaat ggttcgcaaa
    19561 aatttcaata aaagcactcg cacacattac cagaccttca actgatatat cactgcgaca
    19621 ataaaattct ggagaaacac tgatttctga ttctttctta caattgaacg aaaaaactat
    19681 ttttgaatca aattccggta attcttccac aactatgttt tgggattcta ccaagaaagg
    19741 gagtgcctgt tctaatgctt cttctggtgt ttgagcagat agaataattg cttcttgttc
    19801 ttttatttca ttgttctcaa atactttata actttttttc attgttagtt cctctttcat
    19861 tcctctctca attagctaag ccgttactgg tacgccattc tctaaattta gtatcgctaa
    19921 tccttaatgc tcgtcgaata tcaatcattc gataacctat tgcacgaagt tctttatatc
    19981 ttttagcagt tagcgtctct aacgtcattt caggcaatgg gttcgaatca aaaatagaat
    20041 tcgaaccgat tatttgggcc aattgttgta cttgtaacag ctcttctgaa cttaattcag
    20101 tgattgctcg attgcctatc attgcataca ttttctgtcg aatatacttc ttttcttgtt
    20161 tagacaacat ttctatccac tcctattttc attatcattt gttacctcca cttcagtaaa
    20221 tccacaaata aatgaattca tattccaagt accattccct tcaactacaa cttttctcct
    20281 gtcctctttc ggatattcaa gaatcagacc gtttaccaat atgtttttca ctactactaa
    20341 tttgtttgta taccgtggaa ttgctgttcc gatatacttc actttatttc ctacgctaat
    20401 tgtcatttcc tcactcctat tcttttttat ccaaaacttt ttcctgtcct attcctgcca
    20461 acgaatatac ttcactgcat gcttaaacaa aacaaaatgt tgccctgttt tatttccgtc
    20521 ttttacttgt tccacataca gttcatattt ggtcacacct tttaaaacgc cagatacttt
    20581 ttttcatcca ttgtcacaat tagttttttc atcaatcgat tgatactgca ctgctcattt
    20641 ttcttgttca tatagtattc ctttcttttt caaaactagc ctcttttgtt tactagtttt
    20701 catcaatcat caacgattta acagattttt tctttcgcag ggtttctagc gtcagcaacc
    20761 ccaaactcta aaaaagtgcc tttgctccct tgaaaaacga gcggacgatt gtactcgtca
    20821 tcatatgttt ttcatactac ctgatccacg caaataacct ttttttgttc tgtgccacac
    20881 ccatccatac gtatcgcttg agattcacca tactttttta ggtggtttat ccttcctaag
    20941 tccctgttag acacgctgat taacttagaa agtcctgttt gtatccaatt ttcaaagatc
    21001 tcccaaaaga aataagtagg tttacgtgta tcgcttctgt tcaaagtacc tgtttgtact
    21061 tgagtatttc tactcccctc gttcaaggga ttccttccac cagctagctt ttcctttcat
    21121 catcaaatgt tcctgaaaag catttacgtt tcgtgtccgt gtgcttactt attctttttt
    21181 cgatagcgat tatccgctcg ctactcgcta ttttatactt tgtttgttag ctcaaataaa
    21241 aactaacaaa caaaaaaggc tagtttcttt ccgttttatt tttttaactg tcgataaata
    21301 gttgttagtt taattccaag ttctaaaacc aatacgccca acaaaaccac aggaaaaaca
    21361 attggggcaa acaagtaaaa tcggatagtt ttcatgtctt ccctctttct ttttggtgtc
    21421 gctgtggtcg ttcttgccgt tcaatacttc gatacatttt ttattcatta tttgctcgtt
    21481 ttgttttcct ctggactgct tttcttgctt tttgaatact ttatacgctt ttcgtgcctg
    21541 aattaatagg tcttgtcggc gtttcattcg tgagaaaaca atatttgaaa ccatcaaatc
    21601 ctggttcgaa caaccacgat tcgcaataat tttcccatca aaaaaaatag ttttcacttg
    21661 agaaaaaaac agttggaaat cttgttttgt tgctaatttt tttgtcaaaa tcactgtgct
    21721 tttccctgtt gaatctacaa tgattcttcg aacaatccga ttcgataact tccaacgttg
    21781 ccgattaaac aatgttactt cacaacaaat atgacgtaag tgattagggg tcattttcat
    21841 gtgtccaatt cctccaacca attatgcatc gaaattttgg gcaattgtga cagcccataa
    21901 atctgcgttt ccaggtcttc ataactacaa gaacgcttgg cttgaagtaa tgaatattct
    21961 gcttgatctg atagttttgt tgccattgga tctagattca attctcttac acaacattcc
    22021 caaaaattca atagcgctct aaaataaaat ccttttcgat tctgaattgc ttttccttca
    22081 tgacgagcaa ttttttcatt ttgaatacac cgttgatacg ttttgcttaa atcttctgca
    22141 aatgtttccc ctaaaattcg atactcgctt ccatcagata aaccatgtgt ttgattatac
    22201 tttcgttgcc gcttttctat aattcgttta ctgtcgtata tcatgtttag ctgctctttc
    22261 gcttcacgcc attcagctga actgcgaaca atccatgtca aaacatcttt cggaacaaac
    22321 gtactttcgc tgtaatgagc aataaactct ttttgccaat catcagccat ttcaacagga
    22381 ctagtcgtat cgatttttgt atctagattt tctgtatcta gattgttaga agaattatta
    22441 aataattgtg cagaattttc acatggattt ttttcgtcaa aatcatcgaa aatcgctaaa
    22501 ttatcaacag attcctcctc ttcttttcct tgtgcagaat ctacactggt tgagtcaaca
    22561 ggacctaatg aatacacatc attttctgtc gtatctaatt ctcctagata caaccgagct
    22621 ttgtttcttt ttcctgtttg ttgatcaaat cccatatcct ctatttctag tagctcatat
    22681 tctttcaatt cttttttgat tgtattaaca gaacgtctgc aacaattcaa tgcattggct
    22741 aattcttgtt ccgtaaattc aaaataaaca aaaccttgac tatctacttg atttcgttga
    22801 atggctaaat ctaagcgtga ccgaaaaacc atatacgcga tttttgcttt atcagacaat
    22861 gagcgatatt tttcacttgt aataaatacc tttggaaatt gatagtataa ttcattgtat
    22921 ttacgagttg cggtaaatct ttttgttgcc atcgttatta cacctcatct tgtccatata
    22981 ttaagaaaga attttggtgt tggtagacac gaacatcttc cattgtagca atcaattctt
    23041 ttagataaat tcgaccgaat tcaattgtta gtaagtcgta atcttctaat tcatttttga
    23101 tttcttcaat ttcttcttca gacacattga gtaaaatggc taattcttga ttggtataag
    23161 aaatatagac atgtttcttt tcatcaatcc gtttttcaat taaggattct tctaagtact
    23221 cattcagtaa gctataggcg tattttgctt gtgcagtcat cgcataatat tttgacgatt
    23281 ttataaagaa tagttttggc atatgaaaag ttgcttgtgt cagcacatct gttgcgcgaa
    23341 atttgttttt taccatattt tttcctccta gaaaaacacg caaaaaaagc tctgtataaa
    23401 tcgaattaca cagagtttct tcttgttgat atttaatttc taaaatacgt ttcgggtaaa
    23461 aactaaacac ttactaatat ttagattgta gaatcttcgc cttcatttat tcattttaaa
    23521 gagaaaaaga tcaagtgaaa tgtgcccaaa agtttgattc tattaatttt taaacaccat
    23581 tcataatata tttcctataa tgtaaaaagc gtcgatactt attaaagcat cgacgttatt
    23641 tttatagtaa atttatcaaa atttttgcca acattttttc cgattttttt ccctatttct
    23701 atctttcctt atctttctta gttaatatta tttgatgtta atatatcact actaatatta
    23761 aaaaacattg atttatcata ggataaagag tgatagttta atacttaaag agacatatta
    23821 attttattta aaaattgaca ttaatgactc ttaatcagcg ggtcgcgggt tcgagcccct
    23881 cacggcccat tgggtgccaa acccacggaa caggtaaatg ttggtgcgaa agcatttcga
    23941 aaacatttac gctgttcttt ttttattttt caaaataact agggcttttt atttttaact
    24001 caaagtccac tgtagccctt tggactttga gttaaaaaag aagcataatt atgaacagta
    24061 aagtttctaa tataacaata attcgagaag ctttgcaaaa tcatgctgcc aatgaactct
    24121 ctaaacatat aggattaaat ctaagtacta taaaaggtta aaaagtgaag aatatttaat
    24181 agaaaaacta aatttacttg ctgctttccc cttttccatt aatacaacaa tcagagagtg
    24241 tctttttttc gaaaaaatta gaataaataa aagacacgta gtttttttat ctacatgtct
    24301 tccatcgact acaagttatc atttctcaaa acgtatttag cttttaattg attaatcgtt
    24361 tcacgttgtc tacacattgc ctaattaaga ttttcttatt agaagcatca atcccgcaat
    24421 aatacctaaa actccaggaa ttacccacaa taatgttaaa agaaaaaatg gaataataga
    24481 taaaatgagt aaactaatac catagaccac tgttttttga ttttttctta ctaaaaaacc
    24541 tattatgaaa atcattatcg aaaaaactag ggtaattcct ccaaataaaa caacattccc
    24601 gcttagattg actaacatat ctatatcctt actggttagt tctgtagtat ttaaagtagt
    24661 tgaatcaata gaagatagaa acataccacc catcaatgct aaaagactac ataaaatccc
    24721 tgttattcca ctaattaaaa tcaaaataaa ctctgttttt ctcttattca tttaattctc
    24781 tccagattaa gtgttttaga attagaaatt ttttgaccat agttaatagc ttctaatgaa
    24841 cgaataacaa ccactgacgt gttagaaaaa taatccccaa tatgttgttt cttgggcgtg
    24901 aaagcagcca ccaaataccc aatttgaaat acaaaatttt ttaatataaa cctacatata
    24961 cactcacgaa taaagatagt ttgccaactt agctcttttt cctcaaagct cactactctt
    25021 atcccaaaaa tcattttccc aattgtttgt cctgaattca gcttagtcaa aagtacaaaa
    25081 taggttaaat aaatagctaa tgacgctata ccataaaaag taataatgga tttgtcagat
    25141 tccagtccca tgattttgta aaagagacct atagtagcat gggtgatgat gctaatacaa
    25201 attaaatcaa taagaaaagc aaaaaatcgt aaccaaaatc ccgcaaatac ataattaggc
    25261 aaagtttcaa tgccactctt cttattcatt ttatagccct ccatagtaat acattggttt
    25321 tggagtatca gaaaatccaa gtttttcaaa aactgaaatt acacgatctg atgtagttgc
    25381 ctttagccct tgccattcag caatcttatt acctaaccaa gaagaagcaa agccagttgt
    25441 gtcattttta tattctataa ccgtcgcatc agttaatttc ttctcttttt ttagggaatc
    25501 taatgcatct tcagagtaac caatttgatc caccaaccca ttttctactg cttggttacc
    25561 atcataaatt cttccatccg caagttcttt cactgcatta gtatccatat ttcttccttt
    25621 tgctacaaca ttgacaaaac gatcataagc actcatcaca aattcctgta taactttatt
    25681 ctcttcctct gaaggtttgt gttgaggtga catcatatct tttaatgcac cacttttata
    25741 cgtcgcatca gttacgccaa gtttttctaa taagccagaa taatttaaac cagaaataat
    25801 gacaccaatt gatccagttg ttgtttcttc cgtagcaaat attttatctg aaccagcaga
    25861 aatgtagtac cctccacttg ccgctgtatt tttaaatgca gtatagatag gaatatctag
    25921 ctttttaatt tttgccattt ctttagcaat ctcagcactt tcatatattc ctcccccagg
    25981 tgaatttact tctaataaca ctcccttaac cgctttgtct tcttgaattt tctttagctg
    26041 tgttaaaaaa ttctgatggt tatattgttc cctactaaaa agattgcttt cacctgtatc
    26101 tgcaataact ccattgacgg atagtttgac aatcttttta tttgatgcgc cttcttctaa
    26161 cgtttcttct gtgatttcgt tagatccgta aaaaatctta tttattcctg ttagggttgt
    26221 atttgatgct tcctctttat cttttcctgg aattagcgcg acgataatgg atagtaacaa
    26281 taatccgcac gctactccca cagcaatcca tcttctttta ttcatataaa ctcctcctat
    26341 attcttagta aaaataatta ttttttattt agaaacacat cgacaatttg ttgccaatat
    26401 gttttttctc ctacaattaa agacaactct ccagttaatc cataacgaaa cggaaacgat
    26461 tgagttgttt ttaacgttcc tttcactgca taaaaaacac ctttctcagt ggcagtagaa
    26521 ttttctgaaa tttcatctaa agttccgtcg atcgtaatcg gagccccacc tttttgatcc
    26581 aatttaaaat gaaccttcat cccttcttta atataggtac tttcattcgc aggtaaaagt
    26641 gctgtaaatt tcatttttga tttttcgtgg atcggataaa tttctgcaag cactgttcct
    26701 ttggctagtt ctgcttgtcc ctttgtttct tcatttaaat gaaccgttcc agatacagga
    26761 gcttttagta ttccttttga aagttgatcg tccaaacttt tgataactga ctggttcttc
    26821 tcttgttttt catttagctc tgtaactgtt tgttttacag ttgttattgc ttgttctagc
    26881 tcttgctttc ctttttccgt ttgactaatt ctgtcattct cagaagtagt tggtttaact
    26941 aatttagctt tttctgactg atactgttct acttcttttt ttagttgctc aatttgttca
    27001 tttatgcttg ctgaaattgt cagtttcact tgattttttt gatcatcaga aacattattt
    27061 agttgttctt gccaattctc atagtttgcc atcatttctt ttgaaaaatc ttttaatgat
    27121 tgattattac tccaagctac ctgtacttgt tgccattctt gtaatttact atctgaactc
    27181 tcaatttgtt tttctagttg tctttttgtc ttttcgtata cttctaattg cttttgatca
    27241 tttaattcac tttgcttcaa agcgtagcgg agtgattctt tttctgacaa catgctcttt
    27301 aattgattac tgtagccaaa actgtcatcc gttgaaaaca aattttttcc tttacttata
    27361 ctttcaataa atagctgagc catctttatt tgttgagtaa tacgttggtt ttcttgttct
    27421 atctgagcct tttcattctc tattaaagaa caatccaatc taaccaacac atcgtcctta
    27481 tgaacaaatt gattttcttt taggaagttt tccattattt tagcttctaa tggcacttgt
    27541 aacttctcta ttttttcggg gttcaattga gccgttgtcc gaataacaac ctctttttta
    27601 gcaaaggtta aaaacaatcc aataatacat aaaaaaagaa ttacagggta taacacccaa
    27661 cggtagaagc tatgatgttg tttagtatag atactagaat gttcgaacca attattattc
    27721 tgcataaatt gtttccctct cctagatttc ccataatttt tgatagatac catcggcaaa
    27781 acgaagttca tcgtgcgttc ctttttctac caaatgccct tcatggagaa ctaaaatttg
    27841 atcacatgct tttgctattg gtaaatgatg cgcaataaaa ataatcgttt tttcttttaa
    27901 attgaggaga taattcataa ttgcatgttc taacaaggta tctaatccac tagttgcttc
    27961 atctaaaatt aatatgtccg gattttttaa taaagcccgt gctaaggcta atcgttgtcg
    28021 ttgccctcct gaaagattat ttccaccttc ttccaacaat gtctcgtaac gtaatggctg
    28081 ttgattaata aattcattta gttggactat ctcacaaact tctattattt gttcgaaact
    28141 tggtgggcta tctaaaccga atataagatt ctcaatgatt gttccactaa agaaaaatga
    28201 ctcttgaggc aaatatgtca ctcgatcgcg taatatctga aaaggaatgt ctaagtgatt
    28261 aatgtttcca taatggatcg atccttctga tgcttcataa aaacgaacaa ataactttgc
    28321 aagagttgat tttccagaac cactaactcc aactatagcc gttttactgt gcgaaggaat
    28381 tgtacatgaa atttcgttca acgttggggc cttcatgcta tacgagaatg aaacgttttt
    28441 tacagtgata tcttgttgaa ataattgttc agaaagcata tattcaaaag actggttcga
    28501 tttttctgaa tcaatcgcga aaatttcatt taaacgatta tttgcaactt gagccgtttg
    28561 cattttcact tgtaaattaa taatgttttg taaaggatct gtcaaaaata ctagtaaagc
    28621 attgtatgtg ataagttgac ctagactaat tctattatcc ataacaaaat aagaacctag
    28681 ccataaaatt aatgcactgc taattaattg aatcaaatgt ttaattcctt gttgaatatt
    28741 atcaagcgta accgtacgaa atgatttttt cattaattta acaaattcat ggtctatacg
    28801 gttatatact ttctcttcac cgctatatgc tttaattgtt tctattcctt taagactttc
    28861 tatgatacta gaatttacag ctgcaccggc tgccatttct tccctgttcg atttttcata
    28921 gcttttcacg aaagcgaata ttgcaactac ataaaaaggc aaagagcata gagtgattaa
    28981 aaacaacgta ctattctgaa tagctaacgt aaccccaaca agcaacacca taccaatatc
    29041 taaaaatatt gataacgtag cacttgctaa agcatcaata atcttactgg catcaagaaa
    29101 acgagaaata atttcccctg attttctcgt agcaaaaaaa ttcattggta aagttaacac
    29161 atgtttgaaa tactgaagca taattgccat actcattcgc tgtcctaaaa taataagaag
    29221 ataactacga ctatattcaa acaatacccg aaataaatag acagcgatca agccaatgga
    29281 aattatattt aaagttgaac gtgcttgatt agggatgaaa aaatcaagaa taccttgaaa
    29341 ataatatgag cttcctatgc caaaaaacgt aatgaaaatc gatgctaaaa cgatgtggaa
    29401 tactaatcct ttttgtttcc aaattatagg caaaaaagaa cttaatccag ctactttttc
    29461 tttaatagga atataagttt ctctaggcat aggtaataaa agaatacctg tccattcttt
    29521 ttgaaacgct tcaatcgttt ttctattttt tccattagcg ggatctgcaa tccatagtac
    29581 gtttcccttc gttttataaa ttacaacgta gtgcatatag ctgttatcaa ccaatatatg
    29641 tgcaattaat ggtaagataa gatctctttc tttccagatc tcactatctg cttgaatagc
    29701 catacaatca aaagacaatt tttcaaatgt ctttttcaat ccaaatgctg aagtaccctc
    29761 taaatcagtc ccagataggt cccgtaattt atgaatcggg atatcagttt taaaatattt
    29821 taaaatcatc gaaaggcaag caacaccaca atctttttca tcttgttgct gcacaaaagg
    29881 aaatgatttt atcacttatc taacacaact ccttctaaaa aatttttata gaaaaattct
    29941 caatatagct ttaatacagt gaaaatattg tctcataatt ccacaaaaac tttgaaaaca
    30001 gttcttgaac actaactttt tcattcctga atcaaattta ttttggaaat tttactattc
    30061 aagaataaac ttgataagtt tcaggaagat ttttactgtt aatcaaatac gtatgttaca
    30121 gtagcaccgt aaaaaatatt atttaaagaa gcgtgtttaa acatgcaaaa tgtaaaggaa
    30181 ctaagtattg tggaaatgca caaactgttg gtggaggcaa taatgcttgg ggaaaattgg
    30241 ggcaagttgt aggtggactt accacagggg cagtaggtgg tgcaggttta ggaacagcaa
    30301 tttgcggacc agcttgcggt gttgttggag ggttatatgg agctgtcgct ggaggtgctg
    30361 cagctggctg ggatgctcga aaaaaataaa aaaggagaat aagtagtatg catattaaaa
    30421 acacaaaaac aacatttata ttatcttcag aagagttgaa aaatattcaa ggtgggagtg
    30481 ccgtgggagt tttaggcact actttttctg gggcaacagc aggagtaaaa ctttgttcag
    30541 ccggaggacc ttatgcaata gctgcttgtg gtgtaggtgg agctttatta ggtgctggat
    30601 tcagcatgtg gaccggtgcg taaacaatta aaaaatatca aacctcaaga ttggtttgtg
    30661 tcgattacga acataggtat gttctttatc ttttcaaaag tttggaatgg acagaacttt
    30721 ttactaaagc ttcttgtgct agtttttata attatattaa atttttatct tttattaaag
    30781 tcagataaaa attctttgga aatgtaatgg tagagatatt tttacaaaag gacttgcagc
    30841 gctgtagttg gtgctgcagg aggacctatt tcggccgtta caggaggtat tggtaatgta
    30901 cttgtcggat gtgcattaaa gcctgttcct gcaagttaaa tttataaaaa caggaaagta
    30961 attatctcaa atcctatcaa gtacaatcat aggattgcac ttgacaggac aaatttttta
    31021 actaaaaaat gaaaaacact ttctaaaaat tagtagaatt tgccaaatct ttcgcatctt
    31081 ccaccataca tttaatttta attccccttt atggtactta ataaaaacaa gaaaagaact
    31141 attcatttgg cccttttttg gttccagacc atagaaatat tcctccagta actaatattg
    31201 ctgttatgca cgtaactaat aacagctata aaaattggta caactttaat aggattcaga
    31261 aaaatttgca ctagtgaagg aatctatttt attagaaaaa tgcaatctcc ctaaagaaaa
    31321 aaccactaaa cagtgctttc tttcaacttc tccttaattt tttgtgcttt ccctaaagta
    31381 aagtttctgg ccaactctga atcaaaatat agtgaacgtt ttttaaaatc tatttcttta
    31441 acgttaaaga tatttactaa acacgctcga ctaattcttg ttaacatagg atatttcttt
    31501 tcaagctcac taattcttcc ataaaattcg tactgcccat ctttagtaca caatgataat
    31561 ctatgtggta attttgaagc ttctacaaaa taaatgtcat ttatatcaaa agtaaatgtt
    31621 tgagaaccta tggaaaaaac aaatgcttga ttcgtaacct gttttgttgc atctatcctt
    31681 tcttgcgcta ataacaataa ctcaacaatc tcgtttcgat actcatctag tgtttgatct
    31741 tttgttacaa atcctaacgt ttcaactctt ctttttattg ttacgggtaa catctcatca
    31801 tgagtagttg taaaaatgat ttttgcttga acatcatatt ttctgattac ttctgctaat
    31861 tctataccgt taacttcatg atttaaatca atatctaaga aatatattcc ttgctttggt
    31921 cggaattgtt tgaggtattt tttaacttct aacggacttt gtgttttcaa tactatctta
    31981 aatactttat cgtgaaaaag aatgaaatta tcaataattc gttctaactg atttaattgt
    32041 ataatttgat cttcacataa tattattgga taactcataa atcctccttc cctcaaaaca
    32101 acaaaataac ttgagtagta aattgatacg gatcgttttg atagttcaca aacatattgg
    32161 gattcttttt attgatttcc tctatactag ataaacctaa cccttgatgt cccttttttg
    32221 ttgttattcc ttttttcatt agagtatcta tagagagtag tatctctcta caactattcg
    32281 atatcgaaat ttctatctgt ttatcatctc ggtaaatagc tagagacagt tctctatctt
    32341 gactctctat agatgcttca attgcattat cgagaataat tccaagcacc ctaatacagt
    32401 caaatattgg aacaggaact tcaataatag tacttggaca ttcgaattta caacaaatct
    32461 tattgttatt tgcttggtat agcttcgcta tcaacaaact tttcaaatat tcatttttta
    32521 tattttttaa ataacgatat tcaaacttat tggttagata agaattagaa tagatctcta
    32581 attcttttat ttctttttcc aatatcgaat catggttt
//
LOCUS       DANKAZ010000024        32159 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000024 DANKAZ010000000
VERSION     DANKAZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(97..477)
                     /gene="yajC"
                     /locus_tag="PWY67_001052"
     CDS             complement(97..477)
                     /gene="yajC"
                     /locus_tag="PWY67_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL2057350.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(574..1719)
                     /gene="tgt"
                     /locus_tag="PWY67_001053"
     CDS             complement(574..1719)
                     /gene="tgt"
                     /locus_tag="PWY67_001053"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL2057351.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1937..2635
                     /locus_tag="PWY67_001054"
     CDS             1937..2635
                     /locus_tag="PWY67_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2057352.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2803..5442)
                     /locus_tag="PWY67_001055"
     CDS             complement(2803..5442)
                     /locus_tag="PWY67_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2057353.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6061..7092)
                     /gene="queA"
                     /locus_tag="PWY67_001056"
     CDS             complement(6061..7092)
                     /gene="queA"
                     /locus_tag="PWY67_001056"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL2057354.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7285..8460
                     /locus_tag="PWY67_001057"
     CDS             7285..8460
                     /locus_tag="PWY67_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2057355.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8464..9105
                     /locus_tag="PWY67_001058"
     CDS             8464..9105
                     /locus_tag="PWY67_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057356.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9102..10019
                     /locus_tag="PWY67_001059"
     CDS             9102..10019
                     /locus_tag="PWY67_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2057357.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10016..10687
                     /locus_tag="PWY67_001060"
     CDS             10016..10687
                     /locus_tag="PWY67_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057358.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11228..11716)
                     /locus_tag="PWY67_001061"
     CDS             complement(11228..11716)
                     /locus_tag="PWY67_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL2057359.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11917..12183)
                     /locus_tag="PWY67_001062"
     CDS             complement(11917..12183)
                     /locus_tag="PWY67_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2057360.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12183..12446)
                     /locus_tag="PWY67_001063"
     CDS             complement(12183..12446)
                     /locus_tag="PWY67_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2057361.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12922..13107
                     /locus_tag="PWY67_001064"
     CDS             12922..13107
                     /locus_tag="PWY67_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2057362.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13249..15072)
                     /locus_tag="PWY67_001065"
     CDS             complement(13249..15072)
                     /locus_tag="PWY67_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL2057363.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15670..18375)
                     /locus_tag="PWY67_001066"
     CDS             complement(15670..18375)
                     /locus_tag="PWY67_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL2057364.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18681..19628)
                     /locus_tag="PWY67_001067"
     CDS             complement(18681..19628)
                     /locus_tag="PWY67_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL2057365.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19818..20087
                     /gene="rpsN"
                     /locus_tag="PWY67_001068"
     CDS             19818..20087
                     /gene="rpsN"
                     /locus_tag="PWY67_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2057366.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20263..20625)
                     /locus_tag="PWY67_001069"
     CDS             complement(20263..20625)
                     /locus_tag="PWY67_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2057367.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20636..21757)
                     /locus_tag="PWY67_001070"
     CDS             complement(20636..21757)
                     /locus_tag="PWY67_001070"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL2057368.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21773..22123)
                     /locus_tag="PWY67_001071"
     CDS             complement(21773..22123)
                     /locus_tag="PWY67_001071"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL2057369.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22253..23764)
                     /locus_tag="PWY67_001072"
     CDS             complement(22253..23764)
                     /locus_tag="PWY67_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2057370.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24086..25438)
                     /locus_tag="PWY67_001073"
     CDS             complement(24086..25438)
                     /locus_tag="PWY67_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL2057371.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25591..26667)
                     /locus_tag="PWY67_001074"
     CDS             complement(25591..26667)
                     /locus_tag="PWY67_001074"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL2057372.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26863..28608
                     /locus_tag="PWY67_001075"
     CDS             26863..28608
                     /locus_tag="PWY67_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL2057373.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28888..29325)
                     /locus_tag="PWY67_001076"
     CDS             complement(28888..29325)
                     /locus_tag="PWY67_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL2057374.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29309..30682)
                     /locus_tag="PWY67_001077"
     CDS             complement(29309..30682)
                     /locus_tag="PWY67_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057375.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31004..31492)
                     /locus_tag="PWY67_001078"
     CDS             complement(31004..31492)
                     /locus_tag="PWY67_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2057376.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31514..32116)
                     /locus_tag="PWY67_001079"
     CDS             complement(31514..32116)
                     /locus_tag="PWY67_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2057377.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9191 a         6877 c         5939 g        10152 t
ORIGIN      
        1 gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg taggacatct
       61 ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct tctcttctgt
      121 ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg gttcttctgt
      181 tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga ccgttttgat
      241 tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg tccgtttgtc
      301 attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg aatcacctgg
      361 tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg aacgtgacat
      421 aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg ttcccatttt
      481 tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg ctgataacac
      541 tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt tttctttatt
      601 gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat ccatgattgc
      661 ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag aagtcaaacg
      721 aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg tatagttttt
      781 acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat atttcgcatt
      841 tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga ttcgagtagg
      901 caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg aatcagcagc
      961 accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag tgaattctaa
     1021 tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg agtaaccagg
     1081 gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa accctgctcc
     1141 ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca atcctcgttc
     1201 tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt catcgaatgg
     1261 tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga tgttgattgc
     1321 tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat gcacgccttc
     1381 ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg aatctgttaa
     1441 aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta ctaagtcttc
     1501 accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac ccatttcttt
     1561 caactcttct ggggacattg tttttactgt agctaatgtt cctactggca tgaacattgg
     1621 tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag tatgtttttc
     1681 tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta ctttctatat
     1741 aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc aagttccctc
     1801 ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga ataacaaaaa
     1861 aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg ttattaaaga
     1921 aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa aagagagtct
     1981 aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga ttggtattgc
     2041 tactattctt cttattatga ttcctattat cttgtttgcc accaccatat atgatggatc
     2101 agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg gtggcggaat
     2161 tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt atcttgatat
     2221 tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg gattcaaagg
     2281 tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta caacattatg
     2341 gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc aaagctattt
     2401 tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg atacgatcac
     2461 tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag atctttcatt
     2521 tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat ggctcagccc
     2581 atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata tttaaaagag
     2641 agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag gcagtaaaag
     2701 aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca cctccttttt
     2761 gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct cttcttctgc
     2821 ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga tagataaaat
     2881 ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt cttgcgtaaa
     2941 tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt ctaccaaagc
     3001 tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat tgaacacatg
     3061 cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat gctgacttgc
     3121 gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa acaaggttac
     3181 gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag caaagatccc
     3241 ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct ctcttcctaa
     3301 agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca cagctgtaaa
     3361 tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg aaatattaca
     3421 gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc cttgactgac
     3481 agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg cactttgcgc
     3541 cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca acgctggtgc
     3601 atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc aagatttgac
     3661 gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt tgacacgcgc
     3721 atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt ctgacggatc
     3781 attcaagata cctaattctt tcgctatggc actggcagtc accacatgat ctccagtgat
     3841 catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac tttctggacg
     3901 tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca gtgcttcaga
     3961 agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca ctctcagtgc
     4021 ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg aactaaagcg
     4081 cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc ccatttggtg
     4141 aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc gtggatattt
     4201 ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga tggctacttc
     4261 tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca catctgtaca
     4321 tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt cgatattcgt
     4381 tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg tcagcgttcc
     4441 tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg gcaactgtcg
     4501 gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca acgtaacaat
     4561 aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca tgaacatctc
     4621 aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag ccgctaaagc
     4681 gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg ttttttgcat
     4741 agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc caatggcagt
     4801 gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca tattaagttg
     4861 gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa ctgcttcact
     4921 ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca aacgggcatc
     4981 ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca gttgctctga
     5041 ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat tcatatcttg
     5101 caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata ccgagttgat
     5161 cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg tagcaaatgc
     5221 ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat cggacaagct
     5281 tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat tcaatccctg
     5341 cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat ctgcttctgt
     5401 taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc tcctttaaaa
     5461 tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt tacttcaaat
     5521 aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta tatttgcaaa
     5581 cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca aatagacaga
     5641 agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag gtttcaccca
     5701 gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt tggatcacga
     5761 agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa cgtctgaaaa
     5821 ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa aatttttgtt
     5881 taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac aatcatttct
     5941 gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct cggctttttc
     6001 tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct tttcataacc
     6061 ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt caatcgcatg
     6121 ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca tgactaaagt
     6181 cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt catagccagg
     6241 cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa atttcgttcc
     6301 aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc cgccattttt
     6361 acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt cactatgcat
     6421 atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc ctacatgtag
     6481 tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt ctttcgtaaa
     6541 atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga cagtttgata
     6601 gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt ctcccaatga
     6661 ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc cgccgtgttc
     6721 caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc ttgtcccaac
     6781 tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg tattcgttag
     6841 aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac gagcaggcag
     6901 aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa tgatttcatg
     6961 gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac gagaatgatc
     7021 gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat caaaatcttc
     7081 tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta tcatgttttt
     7141 cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct aaataaaaaa
     7201 actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata gttcttcatt
     7261 gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa aatttataaa
     7321 ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga gttcatttgt
     7381 tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa ccgaatgact
     7441 gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat tgatccagtg
     7501 gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc tcacatgacg
     7561 atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga agaacggaat
     7621 aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt agatcgctac
     7681 ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc ccttgcagcg
     7741 aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat cactcgtgat
     7801 tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat cgtatttgta
     7861 acccatgaca tggatgaagc gattaaacta gcaagccgta tcgccatcat gagtgaaggc
     7921 cgtgtgatcc aatttgatac accagaaaat attttacgtc atcctgcaaa cgatttcgta
     7981 gaagagttga ttggagaaga tcgcctacta caagccaagc caaatgcgac aagagtcggc
     8041 gaagttatgc taaatacagc aatcacgatt acaccggaaa aatcattaca agaagcaatc
     8101 aaactgatga gggaaaaacg tgtagatacg ttgttagtta ccgataattc gaatgtatta
     8161 aaaggattta tcgatgtaga aacgttgaat aaaaaacgag gaaaagtatc cagtgtcggc
     8221 gacattttga ataaacatgt tttttacgta aaagaatccg cctatcttcg tgataccttg
     8281 caacgcattt taaaacgcgg gctgaaatac gtgccggtcg ttgatgaaca gaacaaagtc
     8341 gtaggtatcc tgactcgtgc atcattagtg gatatcgtct acgatgtgat ttggggagaa
     8401 gatcccgcaa ctactgatgt tgcagagtca gctactcctg aagaatctaa ggaggtctga
     8461 cctatgcagc agttttttca tcagcacggt tcagaaattc tctcaaaagg ttttgaacat
     8521 ctttatattt caggacttgc tttgatcatt ggtatcatca ttgctgtacc gcttggtgtc
     8581 ttactgacac gttttccaaa agctgccacg attgtgattg gcttaaccag tgccttgcag
     8641 acaatccctt ctcttgcttt attggcattg atgatcccgc tatttggcgt aggtaaatta
     8701 cctgcgatta tcgcactctt tatttattca ttattgccga ttttacgtaa tacgtacatt
     8761 gggatgaaaa atgtcgattg gaattatcgg gatgtggcaa aaggcatggg gatgactgag
     8821 ttccaatcca ttttttccgt tgaattgccc attgctatgc caacgatcat ggctggtatt
     8881 cgtttagctg ccgtttacgt gattgcatgg gctacacttg cctcttatat cggcgctggt
     8941 ggtttaggcg atttgatttt tagtggactg aataattacc aaccagatct cattttggca
     9001 ggtacgatcc ctgtaacaat tttagcacta ttggctgatt ggcttctagg tctcttggaa
     9061 caccggctga caccgatcgc acttagagag gagaatgaag aatgaaacgt atactaaaaa
     9121 tgaccatggt tcttttctct cttcttatac ttgcgagttg ttccttccct ggtcttgcca
     9181 gcaatacaga tgaagacacc atttcgatca caggtggaat cacttcagaa gcacagatct
     9241 tagcaagtct agttgccgga atggttgaac attatacaga taaaaatacc gcaatcatca
     9301 acaatctggc tactacaact atcaaccatc aagcgatgat gaacggagat gcttcaattt
     9361 cagcggctcg ctacacagga acagatttaa cgactacatt gaaccttcct cctgaaaaag
     9421 atccgaaaaa agcgtttgct accgtgaaag atgaatttga gaaaagatac ggtcaaacgt
     9481 ggttcccgtc ctatagtttt gaaaatacgt atgtctttct tgttcgaaaa gacaccgcac
     9541 aaaaatacca tctatctaaa gtcagcgatc tgaaaaatgt agcggatgaa ttagtagctg
     9601 gtgtagatac ttcttggatc aatcgtaaag gagacggtta tgacggcttc caagaaactt
     9661 atggcttctc cttcaattcg atattaccta tgcagattgg acttgtttat gacgcagtcg
     9721 aagcaggaaa aatggatatt gttcttggct actctacgga tggacggatc gccagttatg
     9781 atctagtcat gttggaagat gatcgtcaat tcttcccacc atatgatgct gctccagttg
     9841 tagacaacaa gatattagct gatacgcctc atttaaaaga agccttaaat aagttaggtc
     9901 ataccatcag tacggaaaaa atgcaggaat tgaactacga agcagataat aatctagtgg
     9961 aaccatcagt agtagcacaa cgtttcttac aagaaaacca ctattttgaa gggaagtgaa
    10021 gcaattggat aaaatgaatg tctttcaaca atttttctat tatttccagg aaaatggctc
    10081 ctatatcttt gcacaattta tccgtcactt cctgatttct atctatggtg tcctctttgc
    10141 agcagttgtc gggattccgg ttgggatcat gatttctagg agacgtaaat tagcaaactg
    10201 ggtgattcgt ttggcgaata tcatccagac gatcccttct ctagccatga tctccatttt
    10261 aatcattgga ttaggcctgg gagtcaatgt cgtgattgtt actgtatttt tgtattccct
    10321 cttaccgatc atcaagaata cgtataccgg gatgatccaa gtagacaaaa acatccttga
    10381 tgtcggcaaa ggcatgggga tgactgctag acaacgacta ttcatggtag agttaccact
    10441 ctctgtttct gttatcatgg caggcataag aaacgcgtta gttgttgcca tcgggataac
    10501 cgcgatcggt gcattcgtcg gtgctggtgg tcttggagat atcattatcc gaggaaccaa
    10561 tgcaacagag ggcacatcga ttattttagc aggtgcattg ccaactgcat taatggcgat
    10621 catcacggac tggcttttag gtattttgga aagacggcta gatccagcca gccgtacatc
    10681 acgctgatgt tattgtaaaa aacaaaagct tctgcgacca tttgttggtc gcaaaggctt
    10741 ttttagttct gtcattttta aggcaataat ccactctgat tcttccctcc ttatgggtaa
    10801 agagttgctt ttcttagcta tttatccatt ccgtatactc caacttcata ctgccaacgg
    10861 gttcatatct gcatccaccg actaagtttt tttcttatcc gctaaggcat tttgccaatc
    10921 cattttccca tcactcgtca tggaattcat accacatgat ctctaaatac tcgtctgctt
    10981 gttctttgaa ttgggcatca attcttacat cctttggcgt ggctttcatt taagcctttt
    11041 ccatcagtcc tcgattgttc gatgccttca gctgcatcat caaaactggt gactgccgta
    11101 tcggtaactt cgatcatctt ttatacaatc gggaacttgc catcctcatc cttggctaaa
    11161 aacatatcta gccattccag cttctcgcca tgggaggttt gcggttccta aaaccggtac
    11221 acgattctta tggactgcat ccgtcgcaac tagacttggc gatacgatga tccttcacca
    11281 gaagagcacc cccgtaaacc aattgatcga tatattgcca ataagaaaag acattggcgt
    11341 aaaagatatc gatccctcgt gatgcattgc cgttgatact gctattcatg gccgaaagcg
    11401 ccacgacttt aacttcttcc gtttgagtga cattcatgtt cggcaatgtc gctttggata
    11461 cccgctctgc taaaggaacg acactggtat tgtatttcgt atctgtatct tttccaaaag
    11521 accatgccaa cgaatcctct gggaaccaat aagacgactc cggttgattg tccattgctt
    11581 ttttcaacat acttttggct gtttctcgat aaacgacatt tccaattgtc ttcgcctcac
    11641 tttggcgata atcgatggtc gttgccactg ccaacactaa caacgatgct ccaattttac
    11701 tgattttcct tgtcacactt cttcctcctt gttttacata aacccgtcaa cattccattc
    11761 ctaaactcaa acaatgaccc ttcaaaggtg gagatatagg aacaaattaa agaaatcatg
    11821 caataaatcc aagaaggttg gttttagaac aaaaaaagag tctccttttt ggggagactc
    11881 tttgttaact atctaaaaag gttggctctt ctcacttcac tcataatgtg tccaggcaga
    11941 ataaatttct acaactttat tttcctcatc aactttgtaa accactcgat gctgtctatt
    12001 cagcctgcgt gagtatctgc cttcatgtgt aggtctcagc ttttcaaaag actgcgtggg
    12061 catatatgga tcttccttca atgtctgaat cacttcttca aattgtttct ttaaattcgt
    12121 ttgcttgatt tttcttaaat ccactttcgc agagtttttg attgcgactg tatagttact
    12181 catcaaagag cttcccaatc aatatcatcg atattcgtaa agctactgtc gtctttttcg
    12241 cggtctcgta ctttatctaa agtacctgtt tgctcaagaa aaagggtttc ttgaatcgct
    12301 ctccagtctt ccagcccaat caatacagca ttgtttccac tacgatcact aataatctcg
    12361 atttcagtat gattttcatt tacctctttc aataactgat aaaaattttt acgagcagtg
    12421 ctaggattca tcgttgtatt agccattgtc tctcctcctt tttctgatat aattatagcg
    12481 tacttgatta agtacgtcaa aaaaatacaa taaagcactt aaattcagca aacttttcct
    12541 cttttttaca tagagaaaaa gacccctttt tagggaggat ctttgtccac gctcacttct
    12601 tactccttta cgactagcgg tagccaaacg taaagtaatt ggtaacaacg tatcatcttt
    12661 agcaaaggtc gaatccaaag cctgtgagtc tatgcaacaa caccaataca cgaaggttcc
    12721 tcgttgcagt gtgacttgat tgatatcttg aataacctga ggatacgcac gaaagatttg
    12781 taacggttat tcaaaactga ttttggccgt caagtcttct actgcctcaa aaatgattgc
    12841 ctctttcgtc tcctgtcatt catgttcact atcgatttga tacttgctta gaccgttggg
    12901 tttgtgaatc accaattaaa aatggcactg agttatggta acaaaaaatg gtcacttgac
    12961 gaggctgaga ttcctactta tgaagacaca tggcacttaa gagtcgaggt aaaggaagaa
    13021 gctgaaaata taggaggctg tacattattt ggcaaaggat tcggaaatta tgatcaagac
    13081 atcaaaatca aaatgtttta cagctaaata ggaagaataa taacatttca tacgctgttc
    13141 ccatctttaa tggcttatag agggcacctt tagctgagaa taagacaaaa cagaccctct
    13201 agcttcaatt aagagctaga gggtctgttt ttgattttaa aacttggttt atttcttcaa
    13261 atggtagcta taggtgctga caacgatatt ttcacgccaa gacaatcgca atcttaaccg
    13321 caaacttgtt tggttatgga gaatattctg ccagcgtctg tttggtacaa attgaggaat
    13381 caacacagta gttgtatagt tttgtttcgc tgcattttta cttacgatat ctacataacg
    13441 gataattgga ttttcaatcg agcgataaga agagtggacg attgaaaaac ggacatctgg
    13501 gaaatgcttt ttaaattctg cttcgatttc tttttctttt tcgacatctt cgtctagcga
    13561 aacatgcatc gcaacgacat aatcaccgat cgaacgtgcg taattcaacg caccgatatt
    13621 tacccgtgtc acattcccga ctaatacaag aacggtattc ccgtcataat catgtagttt
    13681 tgcatcattt tctaaacgaa gctgttctgc tactttttta taatgatcat ggatcttata
    13741 aaaaatgaac ataacgattg gcataatgat gaagaaaggc caaatgtccc ctaaccgata
    13801 gacaaacaaa atcgcaatga ttgcatatga gatgaacgca cccgtaatat tggcaatcga
    13861 tttgcttaac catctttttc cttctttttt ccatttgacg accatccctg tttgagataa
    13921 agcaaatgga atgaacacgc cgatagagta taacggaatc aagcgttctg ttgagccatg
    13981 gaaaataaac agcaatacga tcgaacctag cgctagtgtg ataatcccat tggaatagcc
    14041 tagtcgatcc ccacgatctt gatacatgtg aggcatgaat ttatcttttg ccagattgta
    14101 tgcaagaact ggaaaagcag aaaaaccggt gttagcagca actgctaaaa tcagcgctgt
    14161 agcaaattga aggacgtaat acaaaatacc atgtccaaag acagctttac caatttgaga
    14221 caaaacagtg acttcttttt caggaacgat cccataccaa tagttgataa atgtgatccc
    14281 cacgaagaag aaacctaaga tcaatgccat catcgtcaat gtagcagcgg cattttttgc
    14341 tcttggcttc ttaaagaatg gtacagcatt actaatcgcc tccaccccag ttaaagagga
    14401 agaaccagaa gaaaatgctc gtaaaatcaa agcgatagaa acacccggca cagccgctcc
    14461 tggaagtgca gtagcttgca atggcacagc acctgtcacg atattatata aaccaacaac
    14521 gatcagtaac gagatcacaa ggataaatgt gtacaccgga aataatagga acgatgcaga
    14581 ttccctcagc ccacgaagat tcagcatcat cagtagcaaa acgattgtca cagaaatagc
    14641 gacttgatga ccgtagagtg ctggcaccgc tgatgtgatc gcttcagcac ctgctgagac
    14701 agatactgct actgtcaaca tgtagtcgat caataaagag ccaccggaaa tcaagccagc
    14761 attctttcca agattctcac tgctgacgac atatgctccc ccgccatgag gataagcatg
    14821 gatgatttga cgatatgata gcgttaatga aatcaataaa ataataacaa aagcggcaat
    14881 gggtagcgaa taccagatgg ctgcagcaga caaagcgact aaaacgacaa cgatttgttc
    14941 cgtaccatat gcaatagatg ataacgcatc agacgacaat aatgccaaag cagcaaatcg
    15001 ggttaatttg tgctcatcat tttcagcaga ttttaaaggc ttacctacta acaacctttt
    15061 taagtaatcc aatttctaaa ctcctttaca cacaatttta cttcaagttt tatagtcgaa
    15121 aatttatagt aaacaagatg ttatagtacg ctcactgacc ttaaaagtca ataaacttta
    15181 agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc tttaatacag
    15241 caaaaatcta acattttttt atgggctttt tctttgaatt ctataataaa aggatagaac
    15301 ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa gagagatgta
    15361 aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt cacaacctct
    15421 tgataaacgg tgtttaaaag cttgcatctg cggtaataag cccaaccaaa ttaaaaacat
    15481 aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt ttttacaacc
    15541 tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa acggcaaaat
    15601 atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact gatttcacaa
    15661 cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt tcgtcatttt
    15721 ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa acaaaccgat
    15781 catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag attcccgtaa
    15841 caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga atttccctga
    15901 gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga tgataatagc
    15961 gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca tcatgaacgc
    16021 taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat ctactcctag
    16081 taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg tcaaaaatgt
    16141 cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt tctttcctaa
    16201 atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca gacatagtgc
    16261 agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg cattggtttg
    16321 tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt tcaataattt
    16381 gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg ctgctttttg
    16441 gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag cgttcacagc
    16501 tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga tttcattttt
    16561 tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt gctgaatgat
    16621 cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga tggtatccat
    16681 atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag gcatttcttt
    16741 ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg agctgagaag
    16801 cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc caatcgtatc
    16861 gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat tgataccagc
    16921 aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt catcaatagc
    16981 cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt gcagattgct
    17041 tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg tatttccagc
    17101 acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt gttgttgaga
    17161 tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat catcagtcag
    17221 cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg ttgttgccac
    17281 ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta tcgcatcttt
    17341 cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat tttctaaatt
    17401 tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt gttttgcttc
    17461 atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga tctctgctag
    17521 ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga tgatttttgc
    17581 ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac ccatctcatc
    17641 tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct tgatttcagg
    17701 tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt ccaaattttc
    17761 atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg tatcgatgtc
    17821 gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga taaattcatt
    17881 tgtgatttgc gattggatcg aacttgctcc tttatcggtg attttcggtg caatcgcatt
    17941 gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac tagtaaaact
    18001 cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact ttccggaacg
    18061 gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt gtttattttt
    18121 atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa actctgctgg
    18181 cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg gatcccataa
    18241 tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga tgatcagaag
    18301 tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc gaagcgtatt
    18361 tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac tttaccatgt
    18421 atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttttgga catatcacgg
    18481 caattgtctc attgtaatct cttacatatt gagaaataat taaggattac agcctttact
    18541 ttatcatgaa ataaaaggga aagataattt tatgccaaag tttttatcta ttatacgtat
    18601 acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca aaaatgtttc agcttttttc
    18661 gttttttatt cctgtatttc ttatttttcg aatgacagga tgattttccc agcaggagga
    18721 tgtccttcaa ctagctgatg tgcttggatg acatgcttaa gtgaagcaga cagattcatt
    18781 gaaataaaaa catgaaaggc tcctttttgg taggctttga ttatgccagc aaaggcttct
    18841 gcatctagat attcctttct tggaacaaac gattcataaa agccttcctt tttttgccgc
    18901 aaatctagat ctgggaggtc atttaatgca acatacctgc cgcctggttt catgatctgg
    18961 atgccggttt ctcctttgat acttcctttc gtcgcatcga ttacaaggtc tgcctgatca
    19021 gcaaattgca gacctggatt tgttttatca taagcagcaa atgctgaagc tcctaatttt
    19081 tttacctttt cctcattttt actggatgca ctggttaaga ttcggattcc cttttcatgg
    19141 agaagctgga tcagacttga cccgaccgca cctgatgctc ctagtatcat tacagtatct
    19201 gtcggctgga tttccgtcaa atggttgatt agattataag ccgtaattcc tggcgtgacc
    19261 attccggctg cttcttccca actcatttta tctggaattt ttgctacttt tgctgatggc
    19321 aacacgactt cttctccgta agtcccgccg actgcatgga cagctactcg atcacctaca
    19381 tgaacagttg tgacatctga accaatctct gtcacaatac cagcgccatc attacctggt
    19441 acataaggga atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa ggcaacatca
    19501 taaggattca cactgaatgc ttttatttct acacgaacat gtccagaacc gacttctctg
    19561 ggatgagcat cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt aattgcaaat
    19621 cttcgcatgg tcaccatctc cctttttctt taagtttacg tttatgcctt tattttgaca
    19681 aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc ttttttcgcg
    19741 taacgtgtta aattatttat ctaaaagcgt aaagattacg atttacgcta ataaaaaata
    19801 attggaggaa tttgttgatg gctaaaaaat caaagattgc caaagcaaaa aaacagcaag
    19861 aaacgattga acgctatgct gctttacgcc aggaattaaa agcgcaaaaa gattacgaag
    19921 gcttgcgtaa acttccttta gatgctcgtc cagaacgatt gaaaaatcgt gatttaatcg
    19981 atggacgccc tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa ttccgtgaat
    20041 tagcacataa aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc aagtctggct
    20101 gtaatgtaaa ggaggagggc gcatggacca ctccttggat catcaaaaaa gatacgttcg
    20161 cttcttctag cagaaatgct ggtactagaa gcagacgtat ctttttttac gcacagctgg
    20221 tcctttttta tatgtaaagg ctgcatggtt acttcgctat atttatttag taacaggagt
    20281 cagtgcttcg atacccacac taattttcaa tgctcggtca acttctgcca taatgggtcg
    20341 atccaaatgg cagacctttt ctttcaatct tgatttatcg atcgttctga tctgctccaa
    20401 caggataacg gagtctcttt ctattcctgt tccagtagct ttgatcccaa tgtgagtcgg
    20461 aagcttaggt ttagccattt ttgccgttat ggccgcaaca atgatcgtcg ggctaaaatg
    20521 gttacctaga ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct ccgatcccac
    20581 gactggagaa agatcagcaa aataaatatc tcctcttttt accataggat cctccttatt
    20641 cttgatattc tcttggtatc cgctgtccta atccgcaagc cacttcatag tggatcgtac
    20701 ctaattgatc tgctatatcc tgcatagtga tttctttgtc tgcatttttg ccgataagcg
    20761 tcacttttgt tccgacagga aattcttgag gcaatcggat catcagttga tccatacata
    20821 cacgcccaat cgtttcacag tagttccctt ctaccaatag agaaaaaccc tgcatctttc
    20881 gtggccagcc atctgcataa ccgatcggta tcgttccgat ccactctgct tcaggagtga
    20941 tatatgtttc tccatagccg attccttcac ctgctgggag ttttttgact tgaatcaatt
    21001 cagaaaccag ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg tgcccagagg
    21061 gattaagtcc gtacattgct actccataac gaatcatatt gccgattgtt tcatcatgcc
    21121 ataaagccgt tgcactattg ctgacatgta catatcgagg aagttcggga agttttttca
    21181 gcacttctat aaagcgttct ttttgcagat tccaatatgt atcatctgcc tgatctgctg
    21241 tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg actttctttg atgaatgcag
    21301 ctgcctcttt tacttcttca ggtgtacaaa atccgatacg acccatccca gtgtctgctt
    21361 ttagatgaat cgaaagaggc gtctctagct tacttttctc taaaacagca gctgctgctt
    21421 gcaaccattc ccgtgtacct gcagttactg ataaatcatg agcaacagct acagtcagtg
    21481 cgtcgtaagg aaccatattc aagataagga tgggctgagt aaatccagct tctctcaact
    21541 cgactccttc atctaaattt gaaacacaaa agcccgaagc tccacctgca actgctgcct
    21601 cagctgtttc aatagctcca tgaccgtatc cgttcgcttt tactactgca aacaattctt
    21661 ttccttgcgg aagtcttgca acttcatttg ctacattttc agttatagct tttttatgta
    21721 tcactgcttt cgttgggcgg tgccaagcga caaccatcgt ttttccttct ttctagtctt
    21781 ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc catacattgc
    21841 cttcatgagg tgaacgcgtc acaacaggct gaccattttc attttttaaa atctcgacat
    21901 cttgaaaggt aacttttccg atcccagttc cccaagcttt agagaaagcc tctttgcaag
    21961 cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg caaggactgg aatagtttca
    22021 tttcatcaga ggtcaagatt cgtgcaatga attttggttt ttcttcgatc agccgagtga
    22081 tcctaggaag ttcgactgca tcgatcccaa ttccttttat catatgcttt tctcttttca
    22141 ataaatttag aacactctca ttctaccaaa ttataaccat tttgccacaa ataagtccac
    22201 aaataacaaa aatgaaagtt tctcttaaaa aagagaaact ttcattttat gtttaatcgt
    22261 tatttgaacg gattacaaat ccgcgttttt tatcattgtg acgtttaccg ccatcttttt
    22321 ggctgttatt atttttgcta taaccgcctt ttgatttgtt tccacgataa ttaccgccgt
    22381 cacgacgatt acggttgttt ccgccaccac gtttgccatt tttattgtat ccttttttct
    22441 gcattggcaa tgggcgttct ggtgtgatct tcactggcac tgcatcagct ggatcttttg
    22501 ctgtcgtttt tagcaaaagt gcaactaaat cttgtgcaga atattcttct aataatttat
    22561 ctgctgtttg caggtattta tctagaccat tttctgcaag ttttgtttcg atttgttcaa
    22621 ctgcagcacc taattgacct ttgaatgctt ctttttcggt tggtggacgt aaagtcgtca
    22681 tccgtttttt cgtcaagttt tcaatcacat gcaagtagtc catttcattt ggtgtaacga
    22741 aagtcacaga cataccgcct tttccggcac ggcctgtacg tccgatacgg tgaacgtagc
    22801 tttctggatc ttgagggata tcatagttgt acacatgagt gacacctgaa atatctaatc
    22861 cacgtgcagc aacgtctgtc gctacaagga tatctaaatg accgctctta aatgaacgca
    22921 atacgctcat tcgtttttgt tgagacaaat cgccatggat tccttctgca cggtaaccgc
    22981 gtgcttctaa tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg aagacgattg
    23041 ttaattcagg tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt tcgtattctt
    23101 ttgcgcggac atagtattga tcgatcaagt cagctgtcat ttctttcgct ttgatcttca
    23161 catgatgtgg atttttcatg aatttaacgc caatattctt gatagctggc ggcatcgttg
    23221 ctgagaaaag taatgtttgt ctttgatcag gaacttgtga aatgattttt tcgatatctt
    23281 ccaagaagcc catattcaac atttcatctg cttcatccaa aactaatgtt tgaacggttc
    23341 caagtttcaa tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta cccacaacga
    23401 tatgtggacg atctttcaag ccacggatct gacggccaat atctgctccg ccgtaaacgg
    23461 cttgtacacg tacttttttg tcgcgcccta aacgatataa ttcttcttgt gtttggatag
    23521 ctaattcacg tgttggtgcg ataactaaac cttgtaattc gtgacgatct ggatcgattt
    23581 tttctaacat tggtaatcca aatgcagctg ttttccctgt acctgtctgt gcttgtccaa
    23641 taacgtcttt tccagctaat gctagtggaa tcgtttctgc ttggataggc gttgcttctt
    23701 caaaaccagc gcgttcgact gatttcaaca attctggtga taattctagt tctttaaatt
    23761 tcaaatgaat cctcctatat ggttgttaac tgaggatgta gcttttttgc taccccgtcg
    23821 atttgttcgg tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt ttttatccat
    23881 tcttagcccc tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat tagaaaagcc
    23941 aaacgggtcg cgttcagctt tatcattatt atgacatctg gcttcaacaa gttgtttcta
    24001 tggcaaaacc agtccaattt ctcttcatct ttcggcaata ggttattcta acatagtttt
    24061 tcgttttctg caagtttaca gatttttatt tcatttgctg aagttgttct accacttcaa
    24121 ataagcccat gctgttgctg ccttttagca taatactatc tgttgcttca agattttgtt
    24181 tgatagcatc aatcaattta ttcttttctt tttcaaaata gaacacaggt agttgggggt
    24241 atttttggtt caattcttct tttagtgcat gcatttgttc cccataaagg aaaatcatgg
    24301 cgaatttctc aggatcgata tgttcactca tttgcgcatg catatttaaa gactctttcc
    24361 ctaattccag catatctgct aacacagcga ttctgcgtcc ttttgttggc agatttttaa
    24421 aagtatctaa taccagtccc atggctgttg gatttgcatt gtagacatcg ctcaaaatag
    24481 ctgcaccatt tcctgccgtt agccattcgg ttctgttttg agtgatagaa acatgggcga
    24541 gcccttggcg tatctcaggt atggacagtc caaagaagcg cccgatcgta tacgcaatca
    24601 aagcattcgt tacattataa cttccaggca gcgggatcgt aaatgtttct ccttctacta
    24661 caaaatcggt ttgtgctttt ccttttgctg tcacttccgc atgaacatcg ccatttccaa
    24721 taccgaatgt tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc aacggttcat
    24781 ctgcgggcac aacgagtaat ccgtctgata caagtccgtc agtgatttcc attttggctt
    24841 tagcgattcc ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc attgtgattg
    24901 ctgcagcttt tggttgagcc ataagtgaaa gttccgtgat ttcattggcg tgatccatgc
    24961 ccatttccaa aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg taaggaagac
    25021 caatattatt gttgtaattt ccttgcgtct tatatgtttt gtatttttca gacaagacag
    25081 acgcggttag atctttcgtg gtggttttac cattgcttcc tgtaattgca acgacttctg
    25141 cgcctacttt ttccaagtaa taggttgcca attgctgcat ggctttcaaa ggatctgcta
    25201 caggaataac tggaaaattt tttggagctt gttcaacagg caagctccaa aatgaagcag
    25261 atgctccgtt tgtgatcgct tgctggataa attcatgtcc atctcgcgta ccagcaagag
    25321 gaacgaataa acttccttcg gttatcttcc ggctgtcaaa ttcgacattg gtaatctttg
    25381 tcagtgcaga gcattcgctg cccacggctt gtgcaatttc ttgtattgtt agattcattg
    25441 agatctctcc taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg gttcgcatga
    25501 ctctcacttc caagtaaatt tctctattcg tatttagaag ctagaatgaa cgcggttacg
    25561 caatcactcc agcctcttct ttatttctct ttattcattt ttttcaaaaa aagattgacg
    25621 ctgatggtat cgattcattc ctaactggat cagttcttca atcaaatcac cgtatttcaa
    25681 gcccatattt tcccataaga gtgggtacat actgaactcc gtaaatcctg gcatagagtt
    25741 taattcattc aggaataatt catttttatt tgtcaaaaag aaatcgcacc ggctcaatcc
    25801 gcttccacct aacatcgtgt aagctaactt cgcgtactct tgcgcttttt gataaacttc
    25861 ttctggcact tcggctggaa tctgcatttc gattttatta ttgatatatt ttgcttcata
    25921 atcatagaat gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa cgtcttcatt
    25981 tcctaataca gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga ttgctcgaga
    26041 atcatactga taggctgttg ctaaagcatt ttgcagctct tctcggtttt ctgcttttgt
    26101 aatgccgaca ctagaaccca tattcgccgg tttgacaaac atcggataaa gcaaagaacc
    26161 ttcacattga tcaaatactt ttttaggatt ttctttccat tgattcttaa gtactggtac
    26221 ataaggaact tgcggcacac cagcagcttg taaaatatac ttggtcatga ttttatccat
    26281 ggcacatgca ctggtcaata cgcctgcgcc gacataaggc atattcaatg tctctaagaa
    26341 gccttggatc gttccatctt ccccgtttgg cccatgtaaa actggaaaaa cgatggctcc
    26401 ttcttctttg atttcgcccg gattgatcac ttttcctgta aagccttcct ctgtctgtcc
    26461 acttgggtcc catgaaagat gcaagacatc tttgctggca ggtttttctg ttagtaatgg
    26521 acctttgacc cattgtcctt cttttgtaat aaatacgagt tgaacttggt aataattata
    26581 ataaatggca tttaaaactg aaaatgcgga aagaatggac acttcatgct ctgcgctgcg
    26641 tccgccatat agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa atcgttcagt
    26701 gatttactct catattttag cacaaaacaa aaataaacga ataacttatt tataaaaaaa
    26761 cgagccagct tctaaaagaa cgagcgtcat ttattttttg tcagctagat tatttgtccc
    26821 gttttttata aaaagttatg ataaagaaaa ggagggatga tgatggcgca aacaaatcca
    26881 cgcagccgct tggcagaaat cattcgagtg ttcatcaatt acaatgtagt gccaaacttt
    26941 gtacagcaaa aaaatcccga acaggtaaaa aaagcctttg aagaactagg ccccaccttc
    27001 atcaagatag gacagatgct ttctgttcga gaagatttgc tttcttctgc cttcactcaa
    27061 acattcaaaa cattacaaga tagtgtacct agcgacacat tttcaacggt caaaaagacg
    27121 atcgaaacag aactttctct ttcattatca gatatattcg atgatttttc aaaatcgcct
    27181 tttgcctctg cttctatggg gcaggctcat cgtgccaagc tgaagaacgg tgacgcggta
    27241 gtcgttaaga tccaacaccc taacatcgcc gaagagatcc gacttgatct tcaactattt
    27301 gaacgagcga tccctttgat caaatacatt cctgaaacta gcgtagttga tctaaaaggg
    27361 gtactccaag aagtaaaacg ttccttgatc aatgaaatgg acttcttaaa agaaagtcaa
    27421 aatggcgaac aattctatca aaagaacaat ggctggaaag agatccgcag ccccaagatt
    27481 tatgacgctt tttgttcgaa aaaagtgatc gtgatggaag aaatgtctgg taaaaacttg
    27541 aatcatttaa tgaacgccga aaacaaaact gaaactttta tcactggaat tcaaaacaag
    27601 cagctgaaac aagaagttgc aaagctttta gttgagaact ttatgaaaca ggtgtttgat
    27661 gatggttttt tccatgctga tcctcatcca gggaatctgc tattccatgt actaacaaaa
    27721 gaagaacaga cacaagctag ccgaaaaact gaaacagtcc atgaaaaaga gttcggttcc
    27781 tttgcttttc gagcctccac ttctgcagaa gaccctgttg cgccatatac catcaactac
    27841 atcgattttg gtatgatggg gcatctttct gcaggactta ggcaaaaact cacacaagcc
    27901 gttttagcgc tgtatacgaa agatgcttat cgaatcgaaa aagcggtcct tcgactttgt
    27961 cagcaagaag gctcgtttga tgaaagtcgg ttccaccaag aactgacaag ttttcttgaa
    28021 cagtattatg attctcctat tgatgaaatc aacttgcaag aagtattcac acatgtcgtg
    28081 acgatttgtc atcaaaataa tctgcagttc gatcgtgata tcacgctttt gctgaaagca
    28141 tttgggacat tagaaggggt gatccgcgtc ctcgatcctg aagtttccct catggaagtc
    28201 gcttcgcctt ttgctcaaca ttattttctc actcatctgg atgtagaaga tacattgaaa
    28261 caaagcggat tagacttatt ggaagggatg aaggctgcgc ctaagatccc tcaacagctc
    28321 caccatctat tagaaatgtg gacttcggga caaggaaaag tcaatttgga attgaaaaag
    28381 caagacaaat tattgagcag gatcgaaagt atgatcaatc ggcttgtttt cggtatgatt
    28441 ttagctgcat tgatcgtagg gtcctccctg cttgtccaag cagcacctgt agaaaacgca
    28501 gaggtcgttt ccttattagg gatatttacg tatgctattg ccgcattcgt cattattttt
    28561 ttggcaatcg atgcattgat ccaaatgtat aagaagagga aaaagtagaa aaaggttgtg
    28621 acagaaagaa tcagcttcaa gaaataagaa ggggttttac ataaattgtt tctcaaattt
    28681 ttgtgaaaat tcggcttatt tttcgaagag gctacttctg aaacaccgag aataaaaaat
    28741 gtgaaaaagc tgttctgcac ccagtaataa gaacaatcaa aaagggaaca tggaacacat
    28801 ttatcctgtt tccctgttcc ctttttgatg tattcgagca taaataaaat accttcgtta
    28861 cctactatct tctgtacttg gtaaacctta gtcaattgcc ttttttaacg cttgacgaaa
    28921 agacatatga tctttctccg taaagggttt tggacccttt gtccttatgc cagcttttct
    28981 taactttcca ctatgataac gttgttctag aagcgtgccg atattcgctt cgtcaaattc
    29041 ttttccagag tgatggaaca ctcgcacacc cttcggcaat acaagtgaag ctaaaccata
    29101 atcttgcgtg atcaaaaagt cattcttctt aaccataccg acgattttat aatccgcact
    29161 atcagctccg cggtctacgt agacgatttg gacatgatta gcgtattctt tggtcgtata
    29221 atggtccaca ctggtcacga taaccacgtc aagaccatag gattctccta attttatgac
    29281 atcttctttt actggagaac catctccatc aatgaatagc ctcattcttt ttcgccttct
    29341 tccataaatg ataaacagtt actaatccgc tcagtaaaag accaacattg atccattttg
    29401 ccaagataag cagttgtaca ctttctatcg gcactgattc atggttcagc gtgatcaata
    29461 atagatggat tggctctccc atttcttttg ctaaaacaaa caaaagcgtc atcaataaaa
    29521 actggtggat cacactccct ttattttgag tagtatactt gacaacgatc cggcgatcca
    29581 ttggaatagc tgtatcagtt tgataatatt ccttatttag ccagctgcct gttactacgt
    29641 gccagaaact aagcagaaag acacctatcg caaaaccatt tggaatcaag aggaccaata
    29701 aagaagaact cagtccaagc aattgcatag ccaatggaga ccatgttaaa cgtttcaaaa
    29761 tccatggtcc gactattttt gctgcaatca agcctgctgc ataaggcaaa tacataaaaa
    29821 tcgaaagttt ttctaatccg tacagtatgc caacataaaa actgctaaat aaaaaaagga
    29881 aattccctag cattccattt agaaaagtaa acagatttag ccagcttggc aacttccatt
    29941 ccttcttctg gtgtcctaaa acaaacacga acaagatgaa cagcagcaag aaaccataaa
    30001 tagcaagatc aaaaagcctt tcatcgaata acaatcgggc atttctcaaa agaaacaata
    30061 agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc taccagattt cttttgatgt
    30121 ctttgaaatc cagttcgtaa cgcggataat gactaaccgt atggtaggcc atgacgtaaa
    30181 aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt
    30241 gatacaggag aaatagccag atagctgcaa ataagtactt tttcccatcc atattgataa
    30301 aaccttgcgt cttttcgtga aaattgaccg tggtattagc aggtacaagc catgctgcac
    30361 tcagccctag caaaacactt gaaaaataaa ataaaggaaa ataaaatacg cctaatattc
    30421 caagcaatga accagcacca ccaatcagca gagcgatcat cagcaacgtg taactatcta
    30481 gaccaaatcg tagactgttg actaaaaata gtccagtcat ccgaaaggca taaaacagcg
    30541 taaatggcaa aatcgtcagc caattatgcg agtctgttag tcccaaaaat aaaagataag
    30601 gaataaaaat aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa
    30661 atcgtttgag tttttcgaac atgacaatgg ctccttaaac agtcttataa ctaatctgct
    30721 cttcgatttt agcattaaag gggaaataaa ccaattttac agaatatatt cttgtaaaaa
    30781 aaaatacaga aagaagcata agcttctttc tgcattttta cggtacttga ttactcaagc
    30841 agacaagccc ttctccacaa aagtcttgcc cgacacccct gcatccggca ggtgataaag
    30901 atagaaatca attatcctgt gcctccaaaa tcaattagga gctctgtaaa ccagagtcct
    30961 tctcgacccg tcgcataaat ttactttaac acagttccta tgtttatgcg agagcttttt
    31021 tcattttata atcgaccatt tgaaatcctt gaccgatatc tgaaataagt tcttctgtaa
    31081 tctcaaatcc ttcatgctta taaacctcga ttgcttgatg attcccctga ttcactcgca
    31141 aaaagatctc tctttccttt gtttgcgcag caagttcttc ataccagcga aaaactgaag
    31201 aagtcaatcc ttgaccacgt actttattta gcaaatataa tttacttaaa taaatttttc
    31261 catttatctc ttcataagca gtgtaaccga cagtcttttc ttcgcttttt aataaaaagt
    31321 aggacacacc ttctgtcagt tctttttgaa tctgtttttt tgactgataa gttttcagca
    31381 tgtaatccac ttgtttttga ccaatgatcg gcacaaaaac ttccggccat acttcttgta
    31441 tgactgccat cagctcatcc agttcatttt ctgttacttt tattttttcc atacacccac
    31501 gtcctttttt acattataaa tgaccttgcg cttgatggaa taaaaagagc atgatcatgc
    31561 tatacacacc caccgaccac ggtttcaaga aaatcacaat ctgcataaat tccctaaaag
    31621 ataatttcgt caaaccagct actagacaga tcaagtcgtc tggcgcaaag ggaagcagca
    31681 aggtaacaac tagtactttt ttaatatctt tcgtttttct tgtcagcaat tcatcaaatt
    31741 tctgatactt ttttggcgat aaaataagct ggacaaaggc tacgccatag taacggacaa
    31801 gcaaaaatcc gattgcttcc ccaatcacta ggccaataca actatataaa agcccaaatc
    31861 cattgccaaa catcagcatg cccgctactg aggagatccc tcccggcaaa ataggaatga
    31921 tcacttggat cacttgcaac agaacaaata tcatcactgc atacttgcca aattgctgga
    31981 taaacgcttg caaagacgtg ctcgatcgaa aaatcccatg atgataagca taaagaatca
    32041 acacaaagaa aaaaaacaga ccaataatgg gcatgatctg aagacatttt tgtaaaactg
    32101 atcctttttt agacatctat attccccaca tcttttttca tttattaaaa ctgcctcct
//
LOCUS       DANKAZ010000025        31407 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000025 DANKAZ010000000
VERSION     DANKAZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            164..1309
                     /locus_tag="PWY67_001080"
     CDS             164..1309
                     /locus_tag="PWY67_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2057378.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="PWY67_001081"
     CDS             1446..1790
                     /locus_tag="PWY67_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL2057379.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="PWY67_001082"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="PWY67_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL2057380.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="PWY67_001083"
     CDS             complement(3485..4084)
                     /locus_tag="PWY67_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDL2057381.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="PWY67_001084"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="PWY67_001084"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL2057382.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="PWY67_001085"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="PWY67_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL2057383.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="PWY67_001086"
     CDS             7290..8408
                     /locus_tag="PWY67_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL2057384.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="PWY67_001087"
     CDS             complement(8468..9760)
                     /locus_tag="PWY67_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2057385.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9892..10470
                     /locus_tag="PWY67_001088"
     CDS             9892..10470
                     /locus_tag="PWY67_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2057386.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="PWY67_001089"
     CDS             10599..10976
                     /locus_tag="PWY67_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL2057387.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="PWY67_001090"
     CDS             10909..12981
                     /locus_tag="PWY67_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2057388.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="PWY67_001091"
     CDS             complement(13192..14139)
                     /locus_tag="PWY67_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2057389.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="PWY67_001092"
     CDS             14385..14849
                     /locus_tag="PWY67_001092"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL2057390.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="PWY67_001093"
     CDS             complement(14911..15954)
                     /locus_tag="PWY67_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL2057391.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16331..17296)
                     /locus_tag="PWY67_001094"
     CDS             complement(16331..17296)
                     /locus_tag="PWY67_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL2057392.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17530..18375
                     /locus_tag="PWY67_001095"
     CDS             17530..18375
                     /locus_tag="PWY67_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2057393.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18566..19753
                     /locus_tag="PWY67_001096"
     CDS             18566..19753
                     /locus_tag="PWY67_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2057394.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19759..20115
                     /locus_tag="PWY67_001097"
     CDS             19759..20115
                     /locus_tag="PWY67_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL2057395.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20117..20455
                     /locus_tag="PWY67_001098"
     CDS             20117..20455
                     /locus_tag="PWY67_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL2057396.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    20531..20787
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            20850..21884
                     /locus_tag="PWY67_001099"
     CDS             20850..21884
                     /locus_tag="PWY67_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2057397.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21877..22563
                     /locus_tag="PWY67_001100"
     CDS             21877..22563
                     /locus_tag="PWY67_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057398.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22587..23435
                     /locus_tag="PWY67_001101"
     CDS             22587..23435
                     /locus_tag="PWY67_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2057399.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23632..24405
                     /gene="sufC"
                     /locus_tag="PWY67_001102"
     CDS             23632..24405
                     /gene="sufC"
                     /locus_tag="PWY67_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL2057400.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24418..25704
                     /gene="sufD"
                     /locus_tag="PWY67_001103"
     CDS             24418..25704
                     /gene="sufD"
                     /locus_tag="PWY67_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL2057401.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25701..26936
                     /locus_tag="PWY67_001104"
     CDS             25701..26936
                     /locus_tag="PWY67_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2057402.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26923..27393
                     /locus_tag="PWY67_001105"
     CDS             26923..27393
                     /locus_tag="PWY67_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL2057403.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27398..28789
                     /gene="sufB"
                     /locus_tag="PWY67_001106"
     CDS             27398..28789
                     /gene="sufB"
                     /locus_tag="PWY67_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL2057404.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28884..30026)
                     /locus_tag="PWY67_001107"
     CDS             complement(28884..30026)
                     /locus_tag="PWY67_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2057405.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30065..30799)
                     /locus_tag="PWY67_001108"
     CDS             complement(30065..30799)
                     /locus_tag="PWY67_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2057406.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            31031..31306
                     /locus_tag="PWY67_001109"
     CDS             31031..31306
                     /locus_tag="PWY67_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2057407.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
BASE COUNT        10224 a         5741 c         6621 g         8821 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    16081 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    16141 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    16201 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    16261 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    16321 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    16381 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    16441 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    16501 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    16561 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    16621 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    16681 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    16741 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    16801 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    16861 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    16921 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    16981 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    17041 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    17101 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    17161 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    17221 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    17281 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    17341 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    17401 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    17461 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    17521 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    17581 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    17641 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    17701 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    17761 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    17821 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    17881 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    17941 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    18001 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    18061 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    18121 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    18181 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    18241 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    18301 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    18361 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    18421 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    18481 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    18541 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    18601 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    18661 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    18721 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    18781 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    18841 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    18901 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    18961 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    19021 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    19081 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    19141 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    19201 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    19261 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    19321 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    19381 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    19441 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    19501 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    19561 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    19621 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    19681 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    19741 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    19801 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    19861 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    19921 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    19981 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    20041 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    20101 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    20161 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    20221 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    20281 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    20341 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    20401 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    20461 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    20521 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    20581 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    20641 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    20701 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    20761 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    20821 aaaaatcata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    20881 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    20941 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    21001 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    21061 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    21121 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    21181 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    21241 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    21301 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    21361 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    21421 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    21481 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    21541 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    21601 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    21661 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    21721 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    21781 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    21841 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    21901 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    21961 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    22021 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    22081 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    22141 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    22201 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    22261 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    22321 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    22381 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    22441 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    22501 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    22561 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    22621 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    22681 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    22741 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    22801 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    22861 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    22921 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    22981 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    23041 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    23101 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    23161 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    23221 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    23281 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    23341 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    23401 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    23461 taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa
    23521 ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc
    23581 tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc
    23641 ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac
    23701 ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct
    23761 accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg
    23821 tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt
    23881 ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca
    23941 gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag
    24001 ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac
    24061 gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa
    24121 cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc
    24181 gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac
    24241 tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga
    24301 gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt
    24361 atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg
    24421 aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc
    24481 gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca
    24541 ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca
    24601 caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg
    24661 cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa
    24721 ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa
    24781 tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct
    24841 tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc
    24901 gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc
    24961 gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa
    25021 gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc
    25081 aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg
    25141 cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg
    25201 atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg
    25261 gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct
    25321 catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt
    25381 aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt
    25441 gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa
    25501 aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac
    25561 tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc
    25621 ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta
    25681 atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct
    25741 tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa
    25801 agccaaaaca agtactccag gcattagcgt actattacga acacgataac gccaatgttc
    25861 atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa
    25921 aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga
    25981 cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg
    26041 agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa
    26101 aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg
    26161 aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca
    26221 atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg
    26281 gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac
    26341 tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg
    26401 ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa
    26461 tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag
    26521 cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa
    26581 aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa
    26641 aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag
    26701 gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca
    26761 tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg
    26821 aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc
    26881 gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta
    26941 gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc
    27001 ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa
    27061 ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc
    27121 tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa
    27181 gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat
    27241 cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc
    27301 aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca
    27361 gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga
    27421 atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac
    27481 agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt
    27541 ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct
    27601 ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg
    27661 tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga
    27721 agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca
    27781 taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt
    27841 gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa
    27901 caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa
    27961 aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg
    28021 gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg
    28081 ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac
    28141 aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa
    28201 cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa
    28261 cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg
    28321 aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa
    28381 aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga
    28441 cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat
    28501 ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc
    28561 attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat
    28621 ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga
    28681 aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt
    28741 cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag
    28801 gagctagaac ccttgatata taagggttct agcttttttg ttttgcctgt atactagaaa
    28861 tgaccagatt ttgaccagct cttttactga tgagccatca tatcagcaaa gtcttgacct
    28921 gttttcttag gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac tttactattt
    28981 tcatgcccta atcgttgttg aactaccttg acagattctc ctgcttctaa taataaacta
    29041 gcatgtgtat gtctaaaccc atgaggtgta atacgaggca agttatattt tttcaaaata
    29101 tatcttaacc aatcattaac agtgtttgga atgtatagag tgtttttaac tgttgtaaaa
    29161 acatgttgct ctttagagct agtattgtat ccaagtttta aatactcttc tttttgctgt
    29221 aatttccagt tgttgaggat agaaagagtc tcagtatcta aagagataac tcgtctagaa
    29281 gctcttgttt tcggagattg aattataact ttaccaaatt catctacagc aagagtttta
    29341 ttgacatgta gttctttatt aaacgtgtct atgtctttcc actgtaaagc gagtacttca
    29401 ctctttctca tacctgtgta tgccaataat ctgaagaagg cgtattgttt catgttacca
    29461 aaatctttaa aagcatccag taaattatta agctcctctt ttgagtaaaa tttacgattt
    29521 ttctcatact ctttttttcg tggaagaagt gttttttcca taggattatt gtcaatatat
    29581 tgcattgcaa ccccgtagcg taaaatttgt gcagtctgtt ttctcatata atcgtaactt
    29641 tcatatttac tgtgccattc atctaccact ttttgacaat atgggacaga gatattcgat
    29701 agtttaagct taccaaatgc tggaagtatt tgattatttg cgtatcttac tgagattgcg
    29761 attgttgaag ctttaacact ggtacgctga tgagttatcc attcattata caggtcttca
    29821 aatgtcatat caggtgcttt ctttttaaca ccgtataata tttgatgtct gtactcgtca
    29881 taatcttttt gagcttcttg tttgctacta aaacctcttc tcgtagtatt tattttttca
    29941 ccagttcgtg gatcggtggc aatgtgtcct ttatacatcc acgccacttg accgtttttc
    30001 tttttgtact ttgatatttt tgccattaaa tcatctcctc ttagtttaca aggagtactt
    30061 accactagat ggtatcacct cccttcattt ttttcttagt gtattttttc ccaattgatg
    30121 aaatcgtttg ttctagcttg tttatattat cagtaatttc tctcacagct tcttcgggat
    30181 aagacatgtc tttcaattga ataggtagcc attcatctac cattctttct agtagttctt
    30241 ctatactgct agtgaggaaa agaatgttat tgtcaaattc ttctacaata gcattgatga
    30301 ataattcttc tatttttaag atattgaaaa gactaatttt ttcattttta actttattac
    30361 aagtcttgtt agcaataaat tcttgcatct ctggttctaa tttggaatat ctatccagcc
    30421 atgtatctaa attagagtgt actggtggga actgttgacg aagatgctgg aacaggtttt
    30481 tatctggaaa tgttatacca tctagtacaa tgctatctct ataaagaata attcccaaaa
    30541 tatattcttc tacattgcca caaactaact cctctatact catatttccc aaatcagcaa
    30601 ttcgggataa tcgctctggt attggtaaat aggttccctt ctcccattcg tagatagttt
    30661 ttttgtcaat taattgaccg ttgttaatca taagaccaaa tgctggttgt gttaattttt
    30721 tactctttct tatagctttt atttttgttc cgagtatctt ttttcgttct aaataattta
    30781 ttggtggacg ttttatcata ttgaccacac ctcctaaaat tagtatacct caaaaaaggg
    30841 gtgttgcaaa taaattttac aaagctgttg acaccccttt tttatcgagg tattatgtgt
    30901 atgtcgatga actggtaatc attttttgtt tgattaatcg gttaataaca aaatactttt
    30961 attttgagca aaaaatacga agataattct tagaaaattt gttcaagcaa tcacgaaagg
    31021 agggggaaat atgtctctac aaatcattga tagcattgtt tctatacaag ttgaacaaca
    31081 agtgaaagag ataattacag tgctaaagcc tatgctagaa gacattattt ctggagtcac
    31141 tctagagtat atggactatc agcaggcaag tgaatatcta ggtgtatcag taggaacgat
    31201 tagaaaatat gtttctcaat acgggttacc tgttataaaa attgatacgg tcattagatt
    31261 taaaaaatct gatattgata gtttcatgga acggtataaa ggttgagtaa acaaataaga
    31321 ctaaaacata tagtaataaa gagtttgttt ttattatctg tatgttttag tctttaacag
    31381 aattttataa ggaggaaaca caaatgg
//
LOCUS       DANKAZ010000026        30369 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000026 DANKAZ010000000
VERSION     DANKAZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(59..187)
                     /locus_tag="PWY67_001110"
     CDS             complement(59..187)
                     /locus_tag="PWY67_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2057408.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="PWY67_001111"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="PWY67_001111"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2057409.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="PWY67_001112"
     CDS             complement(1674..2729)
                     /locus_tag="PWY67_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2057410.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="PWY67_001113"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="PWY67_001113"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL2057411.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="PWY67_001114"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="PWY67_001114"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL2057412.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="PWY67_001115"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="PWY67_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL2057413.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="PWY67_001116"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="PWY67_001116"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL2057414.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="PWY67_001117"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="PWY67_001117"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL2057415.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="PWY67_001118"
     CDS             10491..12050
                     /locus_tag="PWY67_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL2057416.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="PWY67_001119"
     CDS             complement(12161..14092)
                     /locus_tag="PWY67_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2057417.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="PWY67_001120"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="PWY67_001120"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2057418.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="PWY67_001121"
     CDS             complement(15021..15773)
                     /locus_tag="PWY67_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2057419.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="PWY67_001122"
     CDS             complement(15932..16141)
                     /locus_tag="PWY67_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057420.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="PWY67_001123"
     CDS             complement(16152..17480)
                     /locus_tag="PWY67_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057421.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="PWY67_001124"
     CDS             17616..17771
                     /locus_tag="PWY67_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057422.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="PWY67_001125"
     CDS             complement(17845..18432)
                     /locus_tag="PWY67_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL2057423.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="PWY67_001126"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="PWY67_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL2057424.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="PWY67_001127"
     CDS             complement(20012..21301)
                     /locus_tag="PWY67_001127"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL2057425.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="PWY67_001128"
     CDS             complement(21455..22387)
                     /locus_tag="PWY67_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057426.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="PWY67_001129"
     CDS             complement(22544..23203)
                     /locus_tag="PWY67_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2057427.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="PWY67_001130"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="PWY67_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2057428.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="PWY67_001131"
     CDS             complement(24244..24534)
                     /locus_tag="PWY67_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057429.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="PWY67_001132"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="PWY67_001132"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL2057430.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="PWY67_001133"
     CDS             complement(26820..28175)
                     /locus_tag="PWY67_001133"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL2057431.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="PWY67_001134"
     CDS             complement(28199..29368)
                     /locus_tag="PWY67_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057432.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="PWY67_001135"
     CDS             complement(29365..30252)
                     /locus_tag="PWY67_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL2057433.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
BASE COUNT         8164 a         6583 c         5447 g        10175 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgca
//
LOCUS       DANKAZ010000027        30142 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000027 DANKAZ010000000
VERSION     DANKAZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(373..2100)
                     /locus_tag="PWY67_001136"
     CDS             complement(373..2100)
                     /locus_tag="PWY67_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL2057434.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2093..3286)
                     /locus_tag="PWY67_001137"
     CDS             complement(2093..3286)
                     /locus_tag="PWY67_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2057435.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3473..4117)
                     /locus_tag="PWY67_001138"
     CDS             complement(3473..4117)
                     /locus_tag="PWY67_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2057436.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4211..4345)
                     /locus_tag="PWY67_001139"
     CDS             complement(4211..4345)
                     /locus_tag="PWY67_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL2057437.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4347..6479)
                     /gene="feoB"
                     /locus_tag="PWY67_001140"
     CDS             complement(4347..6479)
                     /gene="feoB"
                     /locus_tag="PWY67_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL2057438.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6476..6949)
                     /locus_tag="PWY67_001141"
     CDS             complement(6476..6949)
                     /locus_tag="PWY67_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL2057439.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7350..7574
                     /gene="nrdH"
                     /locus_tag="PWY67_001142"
     CDS             7350..7574
                     /gene="nrdH"
                     /locus_tag="PWY67_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL2057440.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7733..9892
                     /gene="nrdE"
                     /locus_tag="PWY67_001143"
     CDS             7733..9892
                     /gene="nrdE"
                     /locus_tag="PWY67_001143"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL2057441.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9942..10907
                     /gene="nrdF"
                     /locus_tag="PWY67_001144"
     CDS             9942..10907
                     /gene="nrdF"
                     /locus_tag="PWY67_001144"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL2057442.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10996..12135)
                     /locus_tag="PWY67_001145"
     CDS             complement(10996..12135)
                     /locus_tag="PWY67_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2057443.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12444..13157)
                     /locus_tag="PWY67_001146"
     CDS             complement(12444..13157)
                     /locus_tag="PWY67_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL2057444.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIVLTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13411..14112)
                     /gene="nagB"
                     /locus_tag="PWY67_001147"
     CDS             complement(13411..14112)
                     /gene="nagB"
                     /locus_tag="PWY67_001147"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL2057445.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14346..15878
                     /locus_tag="PWY67_001148"
     CDS             14346..15878
                     /locus_tag="PWY67_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2057446.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16069..17271)
                     /locus_tag="PWY67_001149"
     CDS             complement(16069..17271)
                     /locus_tag="PWY67_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2057447.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17353..18042)
                     /locus_tag="PWY67_001150"
     CDS             complement(17353..18042)
                     /locus_tag="PWY67_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2057448.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18148..18516)
                     /gene="rplL"
                     /locus_tag="PWY67_001151"
     CDS             complement(18148..18516)
                     /gene="rplL"
                     /locus_tag="PWY67_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL2057449.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18569..19072)
                     /locus_tag="PWY67_001152"
     CDS             complement(18569..19072)
                     /locus_tag="PWY67_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL2057450.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19276..19403)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19482..20171)
                     /gene="rplA"
                     /locus_tag="PWY67_001153"
     CDS             complement(19482..20171)
                     /gene="rplA"
                     /locus_tag="PWY67_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL2057451.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20275..20697)
                     /gene="rplK"
                     /locus_tag="PWY67_001154"
     CDS             complement(20275..20697)
                     /gene="rplK"
                     /locus_tag="PWY67_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL2057452.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21040..21708
                     /gene="sdaAB"
                     /locus_tag="PWY67_001155"
     CDS             21040..21708
                     /gene="sdaAB"
                     /locus_tag="PWY67_001155"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL2057453.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21721..22593
                     /gene="sdaAA"
                     /locus_tag="PWY67_001156"
     CDS             21721..22593
                     /gene="sdaAA"
                     /locus_tag="PWY67_001156"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL2057454.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22684..23703)
                     /locus_tag="PWY67_001157"
     CDS             complement(22684..23703)
                     /locus_tag="PWY67_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2057455.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(23876..24781)
                     /locus_tag="PWY67_001158"
     CDS             complement(23876..24781)
                     /locus_tag="PWY67_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2057456.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(24840..25385)
                     /gene="nusG"
                     /locus_tag="PWY67_001159"
     CDS             complement(24840..25385)
                     /gene="nusG"
                     /locus_tag="PWY67_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL2057457.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25486..25656)
                     /gene="secE"
                     /locus_tag="PWY67_001160"
     CDS             complement(25486..25656)
                     /gene="secE"
                     /locus_tag="PWY67_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL2057458.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25680..25832)
                     /gene="rpmG"
                     /locus_tag="PWY67_001161"
     CDS             complement(25680..25832)
                     /gene="rpmG"
                     /locus_tag="PWY67_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2057459.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25994..26638
                     /locus_tag="PWY67_001162"
     CDS             25994..26638
                     /locus_tag="PWY67_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2057460.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26694..27197)
                     /locus_tag="PWY67_001163"
     CDS             complement(26694..27197)
                     /locus_tag="PWY67_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2057461.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27210..27256)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27794..28759
                     /locus_tag="PWY67_001164"
     CDS             27794..28759
                     /locus_tag="PWY67_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2057462.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28858..30030)
                     /locus_tag="PWY67_001165"
     CDS             complement(28858..30030)
                     /locus_tag="PWY67_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057463.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30017..>30142)
                     /locus_tag="PWY67_001166"
     CDS             complement(30017..>30142)
                     /locus_tag="PWY67_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057464.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9191 a         6195 c         5365 g         9391 t
ORIGIN      
        1 atgtttttag actattattc taacattcat taaatttcaa aaaacaattc cttcttgaaa
       61 cgtagcaaaa taattcctga ataagaattt aatcactttt aagtatataa actatgtata
      121 ttctttaata ttttttgaga atgcacttcg tacataataa actttaatca cattagtctt
      181 tcatacctac gatcgatctc tttgactcgc acccataaat tcataatagt ttgtcggcgt
      241 ctttggacgt ttcgaagatg agctacgcat tctctttttt tatagacaaa aaagatagag
      301 acgaacatgg agcttccaaa tttggatatt tgatccaatt ttggaagctc catttatttc
      361 gtgtctgtct ttttattttt tccagctttc gactaattct tggttctctt tgatccagtc
      421 ttttgctgct tgctgtggat cttttccatt gttgatttcc aacataactt tttccatatc
      481 tttttctgac caatggaaat tgtctaatac tttgtaagct tctggctggt cttctttcaa
      541 tccttttcga accatcgtat ggatctgctc ttcgcttccc attccgtttt caggatcttc
      601 taagtatttc agatcatatt tggcaaacat ccaatgaggt gtccatcctg taactacaat
      661 tggttcgtgt tttttgatcg cttgacctag tgcaacggtc attgctcctg aagaagatgt
      721 ctctactttc cagtctttca agttgtcata tttttggatc gtgttttcag cagccgtaac
      781 tacaccagct cctggctcga ttcctatgat tttcttacct gcctgatcgg ttaactcatc
      841 gatcgaattt acatccatgt aggctggcac tgctaaacca acttttgctc ctgtcaaatt
      901 ggcacctagg tcttcgactt gatctttata ttgtgcatat tgtgaaccat gagttttagg
      961 tagccaagct gaaaccatgg catctgactc gccatttgaa acagatttcc acatgattga
     1021 attatctaat ggcgtcatcg ttacatcata gcccatttgt ttcaatactt caccgactac
     1081 atttgttgaa gcaacttctg tatcccattc aacataagat aagttgatac gttttgtttc
     1141 tttggcagat gaaaaactcc ctataccaat cgcacctaag atcactacta ctacagcacc
     1201 aatgatcagt ccttgacgtt ttttactttt ctgtgttgtt gctcctgcag cttttttatt
     1261 gtttggctgg ttcaaatgct gtgtaaaacg gtcaatgatg attgctaaga taactaatgc
     1321 aaccccgttg acaaatccat tcccgacttg tgctctttgc aatgctgata atacgccgcg
     1381 tcctaagccg ggtgcaccga tcatagaggc aataacgacc attgaaagtg acagcattgt
     1441 tgtttgattg atccctgcca tgatcgtact tttagctaaa ggcaattcta gtttgaatag
     1501 tttttgacga cttgtgctcc cgaatgaatc agaagcttct actaattctt taggaacttg
     1561 tcgaatccct aaattcgtaa aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa
     1621 tacccctgga accatcccga taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc
     1681 tggcattgtc tgcataaagt caaggatcgg tttgataata ctttgggctt tactgctttt
     1741 tgccatcaag atccctaatg gtaccccaat gatgatcgag atcacactag ataaaagaac
     1801 aagtgtgaca gtattcatca aatcattcca tagaccttgg ttataaataa accatagacc
     1861 gattaatgtg aacaacgtaa gtcctggacg tttcttagaa ataaagaaag ctgctaccgt
     1921 caataacaag ataaacaaag gagctggaat cactaacagt aaatttgtaa tccccgacat
     1981 cactgcctgt ccaatcgttt gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca
     2041 ttctgtgata ttttctaccc agtttgccac tggtaattga tagttcaata gattattcat
     2101 gttcactcac ctcgcttgtt tctgctaatg cttcaatcac actacctcgg atgacgacac
     2161 cttttaattt tccatcctct acaactgcaa gtggagtcgg tgagtcataa ataatattga
     2221 aaatatcact taccaccatg tctttcccaa tcaccgtcac attttcatct aggaagtcga
     2281 tcaatggacg tttttgcttc ctagcttcta acgcttgatc tgcagtgatg atccctttca
     2341 ggtgtctctt tctgtcaaca gccatcagca tgctgacttc ttcattacgc atccttgtca
     2401 atgcaacgtt tggtccatca gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt
     2461 gagccgtaag cactttggaa cgatcgactt cttccacaaa ttcacgtaca taatcatttg
     2521 ctggattggt caatatttct tcacctgttc cgatctgcat gatttcgccg tctttcatca
     2581 aggcgattct gtccccaatc cgtaaagctt cattcaaatc atgggtaatg aaaataatcg
     2641 ttttttgaac attggcttgc aggtccagta attcgtcttg catttctcgc cggatcagcg
     2701 gatcaagagc cgagaacgct tcgtccatca gcaagatctc aggatcattt gctaaagctc
     2761 tggctaggcc aacccgttgt tgcatcccac cagataactg actaggatat tggtctttaa
     2821 atgaaagcaa actagaattt tctaatgctt tttctgcttt ttcttgtcgt tcttctttag
     2881 gtacaccacg gacttctaac ccgtattctg tattttccaa gatcgttcga tgtggaaaca
     2941 gaccgaagtt ttggaaaacc atattgattt tatgacgacg gacttctcgc aattcttctt
     3001 tgcttaactt cgatacatct tctccatcaa tatagatact gcccgaagtt ggctcaatca
     3061 gacgattcaa tagacggatc aatgtggatt tcccactccc ggaaagcccc atgatcacga
     3121 agatttctcc ttcttttacg tcaaaattca cgtcatacac accaaccgtt gcaccggttt
     3181 tctttaaaat ctccgttttc ggctggtgat cattcatcat atctaaagcg gcttgtgtct
     3241 ttttaccaaa tatctttgtt aaatgtttta cttctacttt taccaaacaa attctccttt
     3301 tttatgtaaa ttcatttttc tttctgaaaa aagtgaccac actatgttaa catcatgtag
     3361 tcactttgtc aaaaatgaaa gaaatttgct tgtccaaact gaaaaaaact tggctaaatc
     3421 tttgtaaata aaaggtttag ccaagttaat atttttttaa tatttcacac aatcattctg
     3481 caagctgatg ctccgggtaa aaaaagtttg tcatacgctg tagtgtatat tcatttccaa
     3541 taaaatgaac gatgtcccct gccgtaaatt tagcataggg gccaggtgaa acaagcagtt
     3601 cttcattatg agaaattgcg ataactgtgg cgccagtcgc ctgccagata ttcaactcgc
     3661 taattgaacg gtccagatgc attgcttttt ctgataaaga taattcaaac ggaagtaaag
     3721 gattcatctt atgtattcgc ttcgtttgat tgaccagctg atccaataat tgagaaaaat
     3781 gattcagctc atcttgttgc ttctccacac ttgccatgat ttctgctttt gtatcttgga
     3841 tggattgtac atcttgatat tgcaatagga aatcttttgc tttttccttc gaagcaacaa
     3901 atgccccact tccatgccgt acttccatga ttccaagatc taccagcaca ttgatagcct
     3961 tgcgggtagt ctcaggagaa gcattgtacg tactggcaag tgttgagcgg gcatggatct
     4021 tctcacccac tttatatcgg ttttcaacga tacgttcggc taaatcaaca gcaatctgct
     4081 ggtagcgcgg gcggacgatt tttttctttc cagacatttt ctttcctctt tcttttttta
     4141 ctttccctca tcttactatg ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg
     4201 caacgactga tcatcgcttg atctgttcct tttttactgg gcaagctgtt tgacagtcat
     4261 cacaattctt accggatttt aaacgagtat agacaattgc gcctgcactt ccaaagatta
     4321 acacacttaa taagaatgtc gccatcctat tcctctcctt ctaatgacaa tatagtataa
     4381 aactggttct tggattttgg ctgacggaac aaaacataaa cgagtcctat catcagtaaa
     4441 atagcaagat acgtccctaa cgctacctgt ccaccctcca ataagtgtcc caactgataa
     4501 atcacaaaac taactaaata agctaaaccg cattgatagc ctatggcgat ccaagtccat
     4561 ttcttggcat tcatttcccg atggatagcc ccaattgccg caaaacaagg cgcacaaagt
     4621 aaattgaagg ctaacaatga ataagctgct gcgggagtat atgtgtgttg tagcacttgc
     4681 cagatttctc tgccattttc ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca
     4741 ataatatttt ctttagcgac caatcctgta atcgctgcga ctgttccttg ccagtttccc
     4801 caaccaagcg gctgaaaaac aactgccagg attcgcccga aaaaggctaa gatactttgg
     4861 tcttcagaaa caggttgagc ataaaaatta taagaagagc tgaaccagat cacgatacta
     4921 gaaacaaaga taatcgttcc tgctttttta acaaaagact tgctgcggtc ataggtttgt
     4981 cttaggaccg ctgaccattg aggaaaatga tatgcgggca attccatgat aaaaggagct
     5041 gtatctccag caaacaaacg tgtttttttc aagctgattc cagagaaaat aattgcagtc
     5101 ataccaagaa aataggcaga aggagctacc caagattgat gaggaaaaaa ggcccctgct
     5161 actaaggcaa taatcggcaa ctttgcagaa caaggcatga aggttgtgac catgatcgtc
     5221 aaacgacgat cttgctcgtt ttcaatcgtg cgactggcca tgacgcctgg cactccacat
     5281 cctgtagcaa tcagcatagg aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt
     5341 cgatccatga caaaagcgat cctcgccata tatccgcagt cttccagaaa gctcaagcat
     5401 aaaaacaata taatcaattg cggtagaaat cctaacactg ctcccacgcc tgcgatgatg
     5461 ccgttcaaga tcagctcctg catccatccc gctacttgcc attcagctaa caatctgcca
     5521 acatgttctg gtaaccatgt accaaacaat gtgtcattga tccaatctgt ccccattgtt
     5581 cctactgttt gaatagccag ataatagatc agccacatca caaatgcgaa gatcggcaag
     5641 gctaaccaac gattcgttgc aatctgatcg attttgtcac tcatcgataa ttggaaagaa
     5701 tcatttgaca caacacagag tgtacatagt tgggtaataa attcataacg ttcattgacc
     5761 agaatcgtct cactatcgtc gtccaatagt ttctctgtta actggatcag ttgctcgatc
     5821 tctttttgct gagaaactgc tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa
     5881 tatttcattg cataccagcg tttcttgccg ctttgttctc ctagtacatc ttcaatttca
     5941 tgtaaagctg cttctaaacg tggatcatat gtacagggaa caggaataga aggccgcttt
     6001 tttgcagact cctgaatctt ttcgattagt ttttcgattc cttgcttttt caatacgctg
     6061 attggtaaaa ctggtacacc aagaccatag gaaagctttt ctagattgat tttttttcct
     6121 tcttttttga tgacatccat catattcaga ccaatgatca taggaatgtc taaatccaat
     6181 aactgcgtag ttaaataaag atttcgttct aaattggtag catcaataat gttgatgatc
     6241 agttcaggct gttcctgaac caaataatct cttgcaatca cttcttctgg tgtataagga
     6301 gacaaagaat agatgccggg taggtcctga atatgaatgt tcggattttt tttcaacgat
     6361 ccttcttttt tttctactgt tacgcccggc caattcccta catattgatt cgaaccagtc
     6421 aagagattga acaagctagt tttcccgctg tttggatttc cgactaacgc gatattcatt
     6481 ctttttcctc cctttgtatc aagattagtt cggcctctga ctttcgtaac gtcagttcat
     6541 accctcgcac tgtcagttcg attggatctc ccagaggggc tagttttcgg ataactattt
     6601 ttacatttcg cgtcaatccc atatccatta accgtcgctt cactgcgcct tgtccgtgga
     6661 ttgctacgac gatcccagtc tctcctgctt ttaattgatc aagagaagtc cagctttcag
     6721 aggaagcagt ctttttattg acaaagattt gacctagtat ttcccgactt actgccactc
     6781 gatgactttt taccagtaaa atggcattgt tgcctgataa attgacgacg acgattttac
     6841 ttccggcaac gatcccgata ttttgcaaat ggcgttttgt tttttcttct gtttcgattc
     6901 gttcgacttg ataaacgaaa ttctgctttg cctgcgctaa ggtctgcata ttgattcctc
     6961 tcttgtgaaa taaatcatgt attaaaatca ttcattatat gttctaattt tcttacgtgt
     7021 tacgcaaata aatgagaatc attctcacat agaatactct cggtaattga gaatgtcaat
     7081 tatacttgtg atattatgag cgaatcttta gtattgatag ataaaaatga gaaaaaaata
     7141 tttcttcata gttttccaca attttttctc tattaaattt gctattttcc cacacgatac
     7201 ttgttttcag ctcttttttc aaaaacacaa aacttagtat tccacagttt ccacaacaca
     7261 atatgtagtg tttgttgttt gactttttta ctaagagttt tatactatct attatagata
     7321 ataagaatgt gttagaggag gcactcacca tgaacgtaaa aatcttttct aaaaataatt
     7381 gtatgcaatg taagatggtc aaacggtttt taacagaaaa tcaaattgct tttgaagaag
     7441 tcaatatcga tgaacagcct gaagcagtag actggttgaa agcacaagga ttccaaagtg
     7501 ttcctgtcat tacttctgat gcagaaacag ttatcggatt ccgtcctgat cagttgcgta
     7561 aacttgccag ctaacatgac taaaagaatc aaaacgtgat acatggattt gaagggcttg
     7621 agacagcacc agtttttgtc gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc
     7681 aatgatttgc aatcaagcat catcaaatac gaaatgaagg ttgggcgtac gtatgagtct
     7741 taaaaattta caagatgtca gttatttcaa gctgaataat gaaatcaatc ggccagtcaa
     7801 tggtcaaatc cctttgaata aggataaaga agctttagtt gccttttttg aggaaaatgt
     7861 caaaccaaat acgatgactt ttctatctat tatggaaaaa atcaattact tgatcgatca
     7921 agaatatata gaagcagaat tcattcaatt gtattcacca tcatttattg aaaagctata
     7981 tcaatttttg gacgagcaac atttttcttt caaatctttt atggctgctt acaaatttta
     8041 ttctcaatat gcgttgaaaa ccaacgacgg ttccgcttat ctggaaactt atgaagatcg
     8101 agtagccttc aatgcgcttt actttgcgaa cggggatgaa gaattagcac ttgctcttgc
     8161 agacgaaatg atccatcaac gatatcagcc agctacacct tctttcttga atgctggacg
     8221 taaacgtcgg ggcgaattgg tgtcttgttt cttgactcaa gtcactgacg atatgaactc
     8281 tatcgggcgc tcaatcaact ctgctttgca attgtcccgt attggtggcg gcgtagggat
     8341 tactttatca aatcttcgtg aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag
     8401 tggtgtcgtt ccagtcatga aattattcga agatagtttc agctattcta atcaattagg
     8461 tcagcgtcaa ggagctggcg tggtttattt gaacgttttc catccagaca ttattgcttt
     8521 cctttcagca aaaaaagaaa atgcagatga aaagattcgc gtaaaaactc tttctcttgg
     8581 cgtgatcgta cctgataaat tttatgaatt gacacgtaaa aacgaagata tgtatttatt
     8641 tagtccatat agtgtcgaaa aagaatatgg tgtcccattc tcttatgtaa atattacagc
     8701 tgagtatgat catctagttg caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga
     8761 tcttgaaaat gaaatctcaa aattacaaca agaatctggc tatccttacg taatcaatat
     8821 tgatacagcg aaccgcgaga atccaatcga tggcaagatc atcatgagta atttatgttc
     8881 agagatttta caagttcaaa cgccttctgt gattaacggc aaacaggaat atgaagtatt
     8941 aggaacggat atctcatgta acttaggctc gacaaatatc gtgaatttga tgcaaagccc
     9001 taactttggt caatctgtgc gtacgatgac tcgtgcattg acatttatta cagatgcatc
     9061 tgatatcgat gtcgtaccac cgatccaaaa cggaaataag ctgaatcata cgattggatt
     9121 aggtgccatg ggcttacaca cttatcttgc aaaagaacaa atggaatatg gctcagagga
     9181 ttcattagac tttacaaata tttacttcat gctgttgaat tattggacac ttgttgaaag
     9241 caacaatatc gctcgtgaaa gaaaacaagt tttccataat tttgaaaaat cagcttatgc
     9301 tgacggcact tatttcgaaa aatacgtaac tggagaattc cagccaaaat cagaaaaagt
     9361 caaagcattg tttgaaggca tttttatccc aactgctgaa gattggaatg ctttgaaaca
     9421 agcagtaatg aaagatggac tgtatcatca aaatcgcttg gcagtagcac caaatggctc
     9481 aatttcatat atcaacgata cgagtgcaag tattcatcct atcactcgca tgatcgaaga
     9541 acgccaagag aagaaaatcg gtaaaattta ttacccagcg ccttatctat ccaatgagac
     9601 gattccgtat tacacttctg cttatgatat ggacatgcgt aaagtaatcg atgtttatgc
     9661 aatggcacaa caacacgtcg atcaaggcat gagtttgact ttattcatgc gttctgaaat
     9721 cccagaaggc atttatgaat ggaagacaac aacgaagcaa acgacacgtg atttgaatat
     9781 tttaagacat tacgctttcc acaaaggaat caaatccatt tattacgtac gtacctttac
     9841 tgatgatgca gaagaaatcg gaagcaatca atgtgaaagc tgcgtgatct aatatcatag
     9901 atagattcgc ttattcatat tttaattaaa ggagcattct catgtcagaa acatattatg
     9961 aagcaataaa ctggaatgaa attgaagata tcattgataa atccacttgg gagaagctga
    10021 cagagcaatt ttggctagat acacgtatcc cactttcaaa tgacctagat gattggcgta
    10081 cattgtctca attggaaaaa gatacagtag gacatgtatt tggcggtttg acactgctag
    10141 atactgtcca atctgaaagt ggcatggatc agttgcgtaa agatgtccgc actccccatg
    10201 aagaagccgt tttgaacaat attcaattca tggaatccgt tcatgccaaa agttattctt
    10261 ctatttttag tacactgaat acgaaaaaag aaatcgaaga aatttttgac tggacaaata
    10321 cgaatcctta tctgcaaaaa aaagctgagc gtatcaatga aatctacaaa cacggtacac
    10381 cgctagaaaa gaaaattgcc agcgtctttt tagaaacatt cttgttctat tcaggttttt
    10441 atactcctct ttattattta ggaaacaaca aattggctaa cgtagcagag atcatcaaac
    10501 taatcatccg agatgaatct gtacatggaa cctacatcgg ttacaaattc caattaggat
    10561 tcaacgagtt acctgaagca gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt
    10621 atgagcttta cgaaaacgaa gaacgttata cagaagaatt atacgatcca atcggctgga
    10681 cagaagaagt caagacattc ctacgctata atgccaacaa agcattgatg aacctaggga
    10741 tggatccact ctttccagat actgccaatg atgtcaaccc aatcgtaatg aacgggattt
    10801 caacaggcac aagcaaccat gacttcttct cacaagtcgg aaacggctat ttgttaggtc
    10861 atgtagaagc gatgaaagac gacgattatt tgattggatt ggaataagga tttattttaa
    10921 ttcaaaaatt tttcgaaaaa taaaaagagt ttagcgtaca gatattttaa atgtacactg
    10981 aactcttttt tatgcttatt cacttgtttc ttccagcact tttggactgt ctaacttttc
    11041 cgaaacatgt tccattcttc tcaataaata gattttccgg atatttaata ttatcgtctt
    11101 aatcactgca tagacaggta tgcaaatcag catgcctaat attcctgcga cacttcctgc
    11161 tccaatcaat accaagatga tcgtcaaagg atggatattc attgatttcc caaataacaa
    11221 cggcttgatc aaatttccat ctaattgttg gatcactaaa atcgacaacg ccatatacaa
    11281 agcttgaaat ggagagataa acaaaccgac aatgattgct ggtaccgctc caataaaagg
    11341 gccgacatac gggataatat tcgtgattcc gcaaatgacc gcaagcaaca agctataagg
    11401 ctgatcgaat attctcattg ccaaaaagct cattacacca ataatcaaag catctaagat
    11461 cgtactgctg atataggcag ataatgtttc gtttaattct tttaccgttt ggcgtaactc
    11521 tttacgaatc gctttcggaa agaagtgaga caacgcatca ataaatttgt gcccatcctt
    11581 gaacataaat agcaaaataa atggaacggt aaataacaaa ataaaaaatt gcatcataaa
    11641 tgaaatgatt tttgagacac ttgcagataa actgactaat atgatattga acaaattact
    11701 aagagataaa tttgctgatg ctaattgttc ttccaaattg aaatttttaa actcttcccg
    11761 ctgcgccaat tcatttaacc atttgcttgt ttcatctgca tacattggca agctctgggt
    11821 caattgaatt gtctgttcca ttaactgagg cactacattc atgacagtta aaacaactaa
    11881 aatgattacg accaaaaacg tcaataaaaa accgaacact ctcgggatcc gatgcttttc
    11941 gagaaaaaca acgattggat cgaacatata atagataaaa ccagcaacaa gcaatggtaa
    12001 aaaaactgcc gaaagcattt tcaatattgg actaaaaata ttcggcattt gtaaaataaa
    12061 gtacacgccg atgatcgtga gtactagctc cactgtccag aaaaataatt ttgattgttt
    12121 aaaacgtgat tgcatacatc tcctccaagg tcctttgata cttctattct atcatttaac
    12181 aaagagttac gcgctgagat tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt
    12241 taatgagtag acgagtactt aaacaaaaac acggtttgga gtagtgacaa aaattatact
    12301 gcagtccctt tttccaaata aacggtgttc aaaagcttgc atcttcgtaa taagcccagc
    12361 caaacgaaaa atataaggaa ctatttttat ttggctgggc ttattacttg atgtaggaca
    12421 tctattttac aacctcagat catttattgc gcaatactga ctaaatgcaa ctttgtgata
    12481 tttttgtgta cggacaactc ctcgatcaca tcggcataag tgagcccttt tggaagatca
    12541 atcgtataga tatttttgta cactcggtag tcatcaacga attttacgtc aaaattcaca
    12601 tcttcgatta cgatattttt ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt
    12661 ctatggataa attggatttc taacttttta gtcgtaggta catggatcac gcgtttcact
    12721 actgtgagta caatagcaat cccgataaaa ctgatgatcg caatggaata gtaacccatg
    12781 ccgatggcga tccctaatcc agcaattgcc cataaagaag cggcagtcgt aagtcccgtc
    12841 accgattgtt tcgtgacgat gatcgttccc gcgcctaaga aaccgacacc actcaccact
    12901 tgagcaatca gccttgcttc atctgaacga atcgttccag caagctgtgg atactctaaa
    12961 gcatctctta atgcaccact agcgatctca acttggatca atgcaatgat acatgctccc
    13021 atacaaacta aaatatgcgt acgcatacct gctggacgat ttttatattg tcgttcaaaa
    13081 ccgattgctc cgccaaatag catagcgatc cctaaacgta aaataatctc tggaattgtc
    13141 aataccatat cattcatcta aacaccctcc agtccaatat atcatgtctg aaaggagcag
    13201 acaaaaaaag tgcaattttt acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat
    13261 agtccttttt atgaataaac ggtgttcaat cagctgaccc tcggtattac tcaacaaacg
    13321 gcaaaatatg gggagctatt tttcctttgt cttctctaat actcaggtca aaacgctgat
    13381 ttcacaacct ctttagccta ctattgaaaa ttacaactta cttgctgctt cttcatcgat
    13441 gatcacaaca acattgtcgt gattttgcaa tacacttgct ggcaaatgat tcgttacagg
    13501 tccttcaatc atccctttga ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat
    13561 ttctttactt ttcataattg aaccgattcc cattgaaaca gcttttgttg gtacatcttc
    13621 tgctttatcg aagaatcgtt tgtttgcatt gattgttgat tctgttaagt caactactga
    13681 agtcgttccg tcaaatgaag caccaggttc attgaaaccg atatgtccat tttgcccgat
    13741 tcctaagatt tggatatcga ttggatgtgc atcgatgatt tgatcatact ttttgcatgc
    13801 tgcttccaga tcttctgctt ttccatctgg tacaaacgtt tctttgaatg gttttttatc
    13861 aaataattgg acatccatga aatgacggta gctttgttca tctgaaccgc ctagtccaac
    13921 gtactcatct aagtttacag aagtcatttc actgaaatcc aaatcgcttg aagtcatttc
    13981 tttatataaa gtaactggtg tgcttcctgt tgccaatcct aatacttttg cacctttgtt
    14041 cataccttct ttgatgattt caaatgcttt ttttccgcct tcttcagcgt tttttacacg
    14101 aatgatttcc atagtgattg ctcctttata ttttggtata gcccaattat agcattcttt
    14161 ttttcttttt acaataatta gtatagacca aattgcattt ttttcatttt cttgacaata
    14221 aataggaatc cgtgttaggt ttcataagat ggttcaatca aacaatccat gttgcatgaa
    14281 aaccaagcaa gtgattcact gtggacgctt tatgtctgca gtttttttat gaaaaaggag
    14341 ttattatgag tatactgaca ttaaacaaca ttacccagca atttggcgac aaaatcctct
    14401 atgaaggtgt cgatctgcaa ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg
    14461 gagccggtaa aagtacattg attaaaatca tcactggtga gatcttacca gatgacggac
    14521 agatccattg gcaaaaaaat attcacaaag gttacctcga tcaatatgtg gaagtcgatg
    14581 aaacattaac aatcgaagaa tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag
    14641 aaaaaattgc ttttctttac caaacatata gcgagacctt cgatgattcc ttgctggaaa
    14701 aagctgggaa actccaaacg gaattagacc aaggagtgtt ttatcaaatc gacaccttag
    14761 ttgctgagat gagcagcggg ttggggatcg atgtcttagg tttagattca ctgttgaaaa
    14821 atctaagtgg tggacaacgt tcaaaggtga ttttagcaaa actactttta gaaaacccag
    14881 atgttctgat cttagatgaa ccaaccaatc acttagatga tcagcatatc caatggctga
    14941 cgcagtttct tcaggatttt gacggtgcgt atatcgtgat ttcccatgac caagaatttc
    15001 ttgatcagat caccacccat atcgcagaca tcgagtttgg aaagattact aagtatactg
    15061 gccatctaaa acaagcattg aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat
    15121 atcatgctca acaaaaacaa atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg
    15181 gttcccgatc aacgatggca aaaagccgac aaaaacaatt agataaaatc gaacggctga
    15241 cgcctccagc ttcagcaagt aaagctgtct ttgattttcc attctcgcct attgtcacga
    15301 cattagcagt ggaaacaact gatttagtca tcggatatga tcgaccgcta cttgcaccca
    15361 tcaatttgac catccgcttt ggtgaaaata ttgccattcg aggcttcaac gggattggaa
    15421 aatctacttt attaaaaaca ctgatcggtg agattgaaaa aataagtggc gatttccact
    15481 tcccagataa cacaaaaatc aattactttt cacaagatct gtactgggaa aatccattag
    15541 aaacaccact acaatattta agcagtcaat ttccaaaagc gactatcaaa gaattacgtc
    15601 gccaactagc taaagcaggg ttagttaatc aattagcaag tgaacctctc actacattaa
    15661 gtggtgggga acaaactaag gtgaaacttg cacagttgac aatgaaccaa ggtaatttac
    15721 tgattctcga tgaaccgacg aaccatatcg atcaagaaac gaaagaaagc ttacaagacg
    15781 ggatacagaa atttccagga acagtaatca tcgtctctca tgaacaagaa ttttatcaag
    15841 atcttgtgca acgagtcatt gagattgaac caaaataaac gtttgattga atagttgaaa
    15901 aaaaggagct gtgacaaaag ttttgtcaca actccttctt ctgaataaat ggtggccaat
    15961 tagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg
    16021 ctattcttat tacttgatgc agaacagctt ttcacaacct cttttcgtct attttttatt
    16081 ttcgagattt ctctttcctt taaaaacaac tgtttgaaag aagaacgcac atccttgaaa
    16141 aacaagcccg ctaaccgtta cacctaacca gccatactgc tgccacattg tcgtccctag
    16201 caatgaacca gccgcacctc caataaagta cataaacata aatacagtat tatttcggtt
    16261 gcttgctgct tcacctagcc cttgtactcg tgtttgattg gcgacttgac caaattgatt
    16321 cccgacgtct aataaaataa tagctaaaag caaaatggcc aaatggcttc cgccaaccaa
    16381 taaagcaata aagcttattg tttgcataac aagtcccata tacacgattt ttctttcaga
    16441 ataagaatct gccagtcgtc ccacgatagg tgcagcaact gctccagaca aaccaaaaat
    16501 cgctaatatc cctgcttcga atgttcccca attgtaggcg ggactgctga tataaaaaat
    16561 caacgtagac caaaagatcg aaaatgttcc aaacatgaaa aatccactga ctgccgcttc
    16621 tcttaataga cgttgagaag caactaaatg cggcaaactt ttaagtgaat caaggtatga
    16681 aacagctgta ctactttgct ggacagaacg cggcattttc cagtaaagca gaagtgtaag
    16741 aattccgact gcacataaag caaataaata gatcatccgc caagaaaaaa gactggcaac
    16801 aataccagat accgtccgag aaagcaaaat ccccacgagt aaaccgctaa gcaatgtacc
    16861 catgaccttc cctcttgctt ttggtcccgc tagtaccgca ccgtaaggaa tgatgatttg
    16921 cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat gcaaataacg taaacgaagg
    16981 cgcaaaaaaa gcggccaaca gagagacggc agataacgct gccattcgaa tgatcaattt
    17041 tggacgatcc accacgtccc ctaaaggaac taaaaacagt aaccctaacg catagcctaa
    17101 ttgggtcaac atcgtcaata tcccgactgc acttgtgcta acagataaac tagctgctac
    17161 ttttgttcca atcggctgga tgtaatacat attggcaaca acaacgccac aagtgattga
    17221 taatagtaaa ataagcgaag ttgttaaagg ttttttttca ttactcttca ttgatttccc
    17281 ccatttccta tcttagttta caacttatgg aaaagattgg aaatagaaaa atacaactat
    17341 tcgccacact tattatttgt gcttttgttt tccttgtttt ttcttgcgat ctttataaat
    17401 gaagacaact aaccctacta gcacagtaac acttgcaacc aacgtgactg cagttttgtt
    17461 atatccgtga aacagagctt ctccaccaca cgcatacaac acagacgaag ggatgactcc
    17521 aatcacgatc gacaatatga tttgctgtag aggcaatttc atcgcatctg cagcaaaatt
    17581 gaccatagaa gatggaataa ccggtatcat atagcctaac attacaccta tttttggatg
    17641 tttcatttgt cggattgctt gcacccaatg attcgtttcg gttttcttat ctaaaaattt
    17701 taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga gcattaccca aaatgttgat
    17761 gatcgcacct gtcagtggtc caaagctcaa accagcaatg actccaacta ctgaagtagg
    17821 aatacctgga acagcacaca tcaccgctgt caatacgatc aagagtatcg atgcaaaagt
    17881 cccatgcgaa cgaatatgtg cgagtagctc tgccttactc gctctcggat ttaaaaataa
    17941 ttggatgtct gtctgatatt ctaaatacaa ccgatagccc actaccagta tcagaatgat
    18001 tcccaacgag atcaatatca atcggattat tttctttttc atatttcctc ctgaaaaaaa
    18061 tctatattca ttaaataata gtttaacata aaaacaaaaa agcaccgaaa tccaacatag
    18121 gatctcagtg cttttaaata gtaaaaatta tttaacagtt acagatgcgc caacttcttc
    18181 aagtttagct ttcaattctt cagcttcttc tttagatacg ccttctttaa ctggtgcagg
    18241 agcgccatca actacagctt tagcttcttt caagcctaaa ccagttgctt cacgaactgc
    18301 tttgataact ttaacttttt ggtctccagc tgcagttaat tctacagtga attctgtttg
    18361 ttcttcagca gcagcagcgc caccagcagc agctacagct acaggagcag cagcagatac
    18421 gccaaattct tcttcaatag ctttaactag gtcgttaagt tctaggatag ttgcttcttt
    18481 taactcagca acgatgtttt caatgttcaa tgccattata atttcctccg tttaattgtt
    18541 cttttttatt taatgcttta tgcggcaatt aagctgcatc tccatcttct ttgtctgcca
    18601 ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg tagtacagat agcaacatag
    18661 atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt gatttcttcc aatgaagaga
    18721 ctttaccttc gatgataccg cctttgattt ctaatgcttt tgcttcttta gcaaattcgt
    18781 ccatgatttt cgctggtgcg actacatctt cgttactgaa tgcaacagct gtaggtccag
    18841 tgaatacttc gtccatgcct tcaaggccag cttttttagc tgcacgtgaa agaatagaat
    18901 ttttgataac tttcatttct acgttagcat cacgaagttg tttacgtaaa cgtgttactt
    18961 catcaactgt taatccgcgg tagtcaacga ctactactga tgcagctgct gtaaattttt
    19021 cgcttacttc atcaacgatt gctgcttttt ttgcgataat tgcttcactc atttttgagt
    19081 ttcacctcct gaattttgat ggaagagttt tttgcactga aaaggcaagt cacttttata
    19141 gttactttgc tcaaccagta ttattcatca cctattcact tcgttcatag tgattctact
    19201 cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc agtattgttc atcatctctt
    19261 cacttcgttc atagtgattc acaataaaaa actctatgcc accgcagaca tagagggact
    19321 attgaatata ttcaataaac gtcctcggta ggaaattaag gcttgatgcc acctactgtc
    19381 ttcggtacaa gttaattatt aacctcaact agcttagcat gctaaactag ttgagtcaag
    19441 aattattttt cagttggatc aatcaacatt ctaatattta attagaaaga agcttggtca
    19501 acatggatac caggtccaaa tgttgttgtg atcgttacat ttttcatgta ttgaccttta
    19561 gctgctgatg gtttagcttt tagcaataca tcatggattg ctgcaaagtt ttcaactaat
    19621 ttttcatcgt caaatgaaac ttttccgatt ggtacatgga tatttccagc tttgtccaca
    19681 cggtaagtta ctttaccagc tttcacttcg tttactgctt ttgttacgtc cattgtaact
    19741 gtaccagttt tagggtttgg cattaagcct tttggtccta atactcgtcc tagacgacct
    19801 acagtagcca tcatatcagg tgttgcaaca actacatcaa agtcaaacca tccgttttgg
    19861 attttttgaa ccatgtcgtc gtcacctacg aaatctgcac cagctgcttc agcttctttt
    19921 gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt taccagttcc gtttggtaat
    19981 acaacagcac cacggatttg ttggtctgct tttttagggt ctacgttcaa acggtatgca
    20041 acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt ttgctaaagc tactgcttcg
    20101 gcaacaggat aaactttact tgaatcaact ttttttaatg catcttgcat ttttttgctc
    20161 tttttagcca ttttggttcc tccttgattg tggttataac ggttgtacct cccacgtgtt
    20221 ctatatcttt caatatagag ccaactgaga agctgctgtc ttagggtgta tagattattc
    20281 tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg cgcattgctg cttcaacgtt
    20341 agctgcgttt aggtcttcca tttttaattc agcgatttct cttacttgat cgctagaaac
    20401 tttcgcaact ttatttttgt ttggttcgcc tgaacctttt tcgattttag ctgctttttt
    20461 caatagaaca gcagctggcg gtgtttttgt aacgaatgtg aatgaacggt cttcgtatac
    20521 agaaataaca accggaatga tcaaacctgc ttgatcagct gtacgagcgt tgaattcttt
    20581 tgtgaatccc atgatattga tacccgcttg acctagtgca ggacctactg gtggagctgg
    20641 agttgcttta cctgcaggaa tttgcaattt aacgattttt tctactttct ttgccacgag
    20701 acatacctcc ttaagtccgt gatgtggtta attggagatg catatttctc ctcccacgtt
    20761 ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa caatgcacac tggtacagta
    20821 tagcaaattt atttgttttt tcaagatttt tttctgctat ttttacattt tgcttgaata
    20881 aacgctaaat ttattctaaa aaaacaacaa tttttcgttc tcatttcttc ttttatgtta
    20941 cacttgcgct agcatggttc aacagatgaa agcaaacaat aacacggttt tttagaccct
    21001 tgtacaaaga ttcctacata acaaaatgag gtggagaaaa tgaaagattt aaggtataga
    21061 agtgtctttg atattattgg acctgtgatg attggtccaa gtagctctca cactgccgga
    21121 gctgctagaa tcgggaaaat catacgtagt atatttggcg aacagcctga ttctgtagat
    21181 atttatctat atgaatcatt tgcaaaaacc tatcgggggc acggaacaga tattgcatta
    21241 gttggcggcc tgttagggat ggaacccgat gatgaacgtt tagctgattc tttgaaattg
    21301 gcatatgaag ccggtatgga agtcttgttt gttcctaaga gtgaaaaagc cgaacatcca
    21361 aactctgtaa agatgcttct aaaaaaagga acacgaaagc tttcagtcac tggcatttcg
    21421 atcggcggtg gcaatatcca gatttctgaa ttgaatggat tcaaaatttc tttaagcatg
    21481 ggtactccta ccttgatcgt tgtccaccaa gatgttccag gtatgattgc taaagtgact
    21541 agtatacttt ctgagaccaa tacgaatatc ggcactatga cagtgacacg ggaatcaaaa
    21601 ggtgaaaatg ccattatgat aattgaagta gatgatcctc aagtagaaga aacggtaaaa
    21661 agactcaaac agctgccaaa tatcgacagc gtaaattatt tcgattaagg agctaaaaaa
    21721 atgtttttat cgattgaaga attagtagat caagcaaaag cttatccaag tgtagcagaa
    21781 ttgatgattg cagttgaaat ggaaatgagt ggccgttccc gtagtcacat catcgagatc
    21841 atggaaaaaa acctctcagt aatggaacaa tcaatcaacg agggaaaaga aggtgttacc
    21901 tccgtcacag gaattaccgg tggtgatgca aaaaaattag atgattattt aaatgaggca
    21961 gactttttaa gtggagaaac aatcttaaaa gctgtacgca atgctgtagc tgttaatgaa
    22021 gtcaatgcaa aaatggggtt gatttgtgct acaccgactg ccggaagcgc gggtgttgtt
    22081 cctggtgtgc tgatggctgt ccgagatcgg cttgatttga cacatgaaca gcaattggaa
    22141 ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa acaacgcttc tatcagtggt
    22201 gcagaagggg gatgtcaagc agaagttggt tctgcaagtg caatggcagc agcagctttg
    22261 gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag cagtagccat tacattgaaa
    22321 aatatgatgg gactgatttg cgatcccgtt gcaggcttag ttgaagtccc gtgtgtcaaa
    22381 cgtaatgctt taggctcttc tcaagctttt atctctgctg atatggcatt agcgggtatc
    22441 gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt accaagtcgg acgtcaaatg
    22501 ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg ttacgccaac tgctaaacga
    22561 cttgcggaag agattttaaa taagagggcg tgataaagct ccttaatttc gagtattaag
    22621 agattagtta tcttcataac aaaaaacgaa gagaggacta attgcccttc tcttcgtttt
    22681 ttattaatcc atcaatttcg tattttttac ttcatcgatc gtttttgttc ctgcaagttg
    22741 catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca gatgttactc cttctgcccc
    22801 acctagattc aacccataaa tcactggtcg gccaatcgca actaagtcag caccacttgc
    22861 cagagctttg aagacatgct ctcctcgacg gacaccagag tcgaaaatca ctggtacacg
    22921 tttgttgact acttcggcaa tttttggcaa tacttcaaag gacgcaggac ctccatctag
    22981 ttgacgtcca ccatgattgg atacccagat accatctgcc cctgcagaga tagcgattac
    23041 tgcatcttct ggcgattgta ttccttttac gataactgga agatgggtga tttctttgat
    23101 tgttttgatg tcatctggtg tcaagccttg ttttgcagct gcataaattt ctgcgattcc
    23161 ttttccttcg ccattaccac ttgcactttg ttcgctatac gcagctaagt taggcattgg
    23221 taatggaaat tggaattgat tgatgacgtc ttcttcacgg taaccaccga gcgttgaatc
    23281 tgccgttaaa atgatagctt tagcccctgc ttctactgct ttgttcaaaa tgaactcatt
    23341 gaatctgtcg tctttactca tatataattg gaaaaactgc ggtgcgcctg gcgctgcatc
    23401 ggctgcgtct ttgattgtcg tatttgcata agtgctgatg gaaaagattg atccagcagc
    23461 agctactcct ttagctgtat cagcttcccc tttttcatgt gccaatcctt gggccgctga
    23521 tggcgcttgg ataatcggtg tatctaattc aattccgaaa acagatgtat gcaaatcggc
    23581 tgagtcgatt ccgcgtaata tccgtggcat gattttcttt gtattaaaag aagtcgtatt
    23641 ttctttcatc gtccactcat cttcagaacc accgcgaata tagccaaaag ctcctgcttc
    23701 catgcgctct tttacacgac cttctagtta ggcaatgttt acaatatcga ttggatgttc
    23761 ttcattactt gtatgataag tcatagtaga tccttctttc atcttcttga ttacatataa
    23821 atcatactaa acttacaaaa agtaagcaaa catttactct cagatacttt tatcgctatt
    23881 ctttccaaat aaatcgactt acaagctgat ctacttcttg gttttcatga agcatactat
    23941 gagaggcatt ttcacctgtc actacttttt cctctatttc atttccgcgt tgcttcaaca
    24001 acgcatagac cgctaatgca ctgtttaacg gtactgttcc atcactcaag tctgtttcat
    24061 ctagttgacc agcaatcaag aaaaatcgag tggttatagg aatattaccg atcatttgct
    24121 ggtaatcttg atagcggtta gaaacgacag caggcccctt tgctaactca tctgtcagag
    24181 actgcgcact atcatcgaca aaatcattaa aaggcgcgcc aattgctgtt aatgtctgaa
    24241 cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata gcgtagtgca ctaactcctc
    24301 ccatggaatg ccccactatg ttgactttat ctatgccgta gttttctttc agatacaaaa
    24361 gacaagtttt gatccattct gcttgattcc actcgttatt tttattatct tcaaacaata
    24421 cttggaccat cggattgtct gcttttccag acaaatctcc tgttgcttgt atttgtccat
    24481 ctgttcctac tgttaaaacc aattctttta ctgcttgctg atttttttcc aaacgccgca
    24541 acatcccttt aaaggaattt tctgtcccgc tgtagccgtg gaaaaaaagt gttggaatag
    24601 tggttttttc tatcacttta ctggattctg ccgttttttc tttatcagaa acttcagtag
    24661 tatttatctt cgtcgtctgg caaccactta acgcaaacaa actgataagg actaaagctg
    24721 ctgcagttgc acgtttgcat aaatttttga ttggttctgt catacacctc ttcctttcca
    24781 tatagaaaaa agaaagactt gaaaggccaa tcatatgatc gacatttcaa gtctctttat
    24841 taatcgattt tatctacttg atcgaaatca agttctgtac tggtttcacg tccaaacata
    24901 tcgatgttga ctttcaattt ttctttttct ccgtcaactt ctgttacttg tccttctaat
    24961 ccagcaaatg cgccttcgat gattttcacg acttcgccaa cttctacttc tagatcattt
    25021 tgtcgcaggc tcataccgat agaacgcaag atatgattga cttcttctgg aagtaatggt
    25081 gctggtttgc ttccggctcc gtgtgaacca acgaatcctg tcacaccagg tgtgttgcgg
    25141 acaacatacc atgaagcgtc tgtcatgatc atttcaacaa gtacgtatcc tgggaatgtt
    25201 ttatgaacga tttctttttc tttcccattt ttgacttcag tttctttttc ttcaggaaca
    25261 acgacacgga aaataaagtc ggccattttc atactttgcg cacgagattc gatatttgct
    25321 tttactttgt tttcatatcc tgaatatgta tgcaagacat accagttttt ttcaaaagat
    25381 tccattgaag gctcctcatt tcattgtaaa ataaaaaaac ctcagtacac ccgaagtttt
    25441 tctttcgctt cattatagca ctaaaagtac gttgtggcta gcgagttatt ttaaaatcca
    25501 gccaaataag ctttggatgg ctgtatccat tacgtaaaac atcaatccga aaataataga
    25561 agtttcgatg actactaacg tatctttacg gagttgtttt ctgcttggcc aagttacttg
    25621 tttgatttca tcttttacgc tttttagaaa tttcatgaga caacctccct tgctactgtt
    25681 tatttcgttt ctttatgtaa tgtatattga ttacaatatt tacaaaactt attgatttcc
    25741 aaacgttcgc cgctttttcc ttcactgact gattttgtgt agttgcgaga cccacaaacg
    25801 gaacaagcga gtgctgcttt tttctttgcc atttacttct gcccccttgc tgagaatcag
    25861 acttatactt cataacagta tcacgtaact ttttacttgt caatttgtga aataaactat
    25921 ctattgtttt ggataaaatt atgatatgat taatttgaaa ttaagctaaa atgagacaat
    25981 tggaggtata gcgatgctat taaaaacact tgtatataag aaaaaagatc tgacaaccgt
    26041 tacagagact agtacattag aagaagcatt aaaaatttta gaaacttccg gataccgttg
    26101 tgtgccgatc ttagattctt caggtaatat tttccgtgga aacatttaca aaatgcatat
    26161 ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct gtcactcatc ttttgaaaaa
    26221 cgctacaaag ttcatttacc tggatacttc tttcttcaaa gtattcttta cgatcaaaga
    26281 gcttccttat attgctgtcc ttgacagtga aaatcatttt tatggtattt tgacacatag
    26341 tacgttgctt aatatgctct ctcaatcttg gagcgtagaa caaggaagct atgtactgac
    26401 aattgcttcc acaggaaagc aaggagattt ggctgctatt gcaaaaatca tttcaaaaca
    26461 cagcagcatt gccagctgta ttactcttga tatcgaaaaa gacgaataca ttcgacgtac
    26521 attgatcacg ctaccggcta acactgaagc gaaagtatgt gaaacgatcg tcaaccactt
    26581 agagaaaaag aattataaag tggtagaaat tgaaaatttg caaaaccaag aagcataatc
    26641 aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt tcgcttttta taattagaca
    26701 cgcttttcaa aatcaagcgc gcgatctaaa taatacatca atggtgctga aacagccatg
    26761 acgataaact cacttaatgc aagcgtgcca taggttgtcc aaaatgcttg gccacctgtt
    26821 ggaactaaca tataagcaat cagtcccatg ctgatggtaa agaagatcgt gttcaatact
    26881 aaacgcatct ttacattttt cactttgttt tgcaataaag ctgtcaggcc taaagaaagc
    26941 aatgtttggc cgccaccaaa aaccacatcc atgataccaa aaccaaagaa ggcattatag
    27001 acgatgaccc cgcctaaaac tccccaaagc aatttacggt taaagacaac taaatgattc
    27061 aaactttctg aaatacgaaa ctggatcggc cctgaggata caggcgcaac taaaatagtt
    27121 aacgctagat aaagagccat gatgatgcca tttacaacga ttacggataa ccgttgtctg
    27181 cttttcgttt tttgcatttt ctctcctctt ttctcctagt ttttttaacg cgggatggtt
    27241 gatgaaccgc ggttatgtga aacaaacaat aattataaca gacttttcat ttatttcaat
    27301 ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat gtaaacgttt gctaaaaatc
    27361 gtgggatatg gtataaagta agtgtaaggg gtcataagaa ttcccagtaa agcagacgaa
    27421 gtaggatctg caagaacaga attgttgtga aacttaaata aataaaaaag gatgtgggaa
    27481 tttgagcaac ctcttactag agaacggtct agcaagcagc tgaagatcta agtggacatg
    27541 ccgaaccttg gaacttctcc ttacgtaagt aatggatcat gcgcttgcta ggcttttttg
    27601 tgtttttcgg tcttcggacc tttttcattt tcctagactt ctgctataat aaccaagaat
    27661 taattttgta acagattttt ttgaaaaaac aagtttcttt cattcaaaaa atagaaaaag
    27721 cttgctaatt attggttaac cagcaaacag gagtattaaa atatcttgtt ttattgaata
    27781 gcaggaggaa atcatgaaat tccattatca tatattgatc aatccatcgg caggcagcgg
    27841 aaatggctat aaagtagcag aaagaatttt accggtatta aaaaataagc atattgacta
    27901 tacacttcat tatagtgaat acaaaggaca cgatgctgag attgctgaaa ctttggcaaa
    27961 agaaacattg attccttgga tagaagaaga acagaaaaca atcgatactt tccccctttt
    28021 gatcatcgta ggaggtgacg gcaccttaca tcaggtactt gatactttct atcagatgga
    28081 agtagaattc ccagttgctt atattccagc aggttctgga gatgattttg ctcgaggggc
    28141 aggccttcca aaaaatccca aaaaaggact tcagctgatt ttagcagctc aatcaccaga
    28201 aaaagtccac ataatggctt atgaagaaaa aataagtgaa aaaaaaggaa tagctgttaa
    28261 taatttcggt ataggattag atgcagctat cgtccatgcg acaaatcatt cttccacaaa
    28321 aaagcgattg aacaaataca acttaggatc attttcgtat ttattctcta ttctccgtgc
    28381 tttattcact caaaaaggat ttccaattct ggtagatgca ggaggaaaac aattaagttt
    28441 ttcaaacgca tttttatgta cagcaacgaa gcatcctttt tttggcggag gaatcgcgat
    28501 tgttccaact gctgatgcgt ctaaaccagt cattgatttt gtcgtggtcg aacgaatcaa
    28561 tttctttaaa atcttgtggc tcattctttt acttatacag aaaaaacaat tgaaatcgaa
    28621 atatttccat cactacacaa caaacagatt acgtatcgtt tcaacaacac cgcaacatgg
    28681 tcaagaagat ggagaagaaa tggaaaaacg accttttgat atcacaataa ccacaactaa
    28741 acagttgttt tggtgttaat aaaagaggtt ttgtcagaag ctacatctgg aacaccgttt
    28801 attcggaaaa aaggactgtg acacgacttt tgtcacagtc ctttttttac tatgcagtta
    28861 attctttgcc caattgggtt gccatcgata gtttctttct ttacccaaaa taaattggat
    28921 cattgaaaac accgcagcaa aagcaactaa gacacaacca ttgaaccaaa aagattctgc
    28981 aaatgtttga ttgtaactga gtgtttcact gatcaagtta ttattttgaa caaaccaata
    29041 tgttggtgtg aatgccgcta ttttgctaac catttctggc aagtattctg ctggtacaaa
    29101 caccccaccg ataaagcaac tgcctgtgat aaaaatatct ttgatcccac cgctggtttc
    29161 tgagtttttt accaatgaag tgatacaagc cgaaaagcta atgatcggca ataaaaacaa
    29221 gaaagtattt aaaatatgat accataccgt tatatcccac gaggtgtgga tcactaacag
    29281 aaccattaga agtaagaagc cactgaacaa cagcagcgag taactaatca aactaaaggt
    29341 tactttgcga ttcaagtttc gtttggtaat gggcgcactc tggtttcgtt tactgatctc
    29401 tttgcgatta aatgctagat tgatgacgct gaaaccgcta aaaatggata gaaacaaccc
    29461 ataagacaag aaattaaagg ttgtgcccgt caacagcttt ttcatccgct gactatatcc
    29521 cgcacttagc tcaacttctc cagtaaccga gagtgccatt tgtgtctgtt ccatagctgc
    29581 atcgattgtt ttatcttctt cttgaaacag aatcagtgtt tgcaaaaagg tgttcacttg
    29641 ctgtgtaaca actgttttag aaaaggcatc tgggcgactc tgtatcttga tcgtcggcat
    29701 ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga tgtaagataa tgtttacacg
    29761 attaaaataa agagcatcat cgatttcttt ttgtgaggtt ccatccaatg taacgactgt
    29821 ttccttgtca ttcaaatatt tacgaaggcc atcgactaca ggcgactctt cttcactaat
    29881 caaggcgatt ttcgcatctt gcagagaggt attttcaaca tcaatatttt gcgcaaaaaa
    29941 gaatgtggca acaacagtga taattacacc tatcagtaaa ttcgatttat ttgctttaag
    30001 caatgtccaa atcgttttat aaagcgtcat aacgcacccc tctttccatc caagcatttc
    30061 ctaacgcaaa aaggaaaata aatccgatca accatagaag attggtataa aaactagcca
    30121 aatcagtgta gaaataaagt tt
//
LOCUS       DANKAZ010000028        29983 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000028 DANKAZ010000000
VERSION     DANKAZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            139..477
                     /locus_tag="PWY67_001167"
     CDS             139..477
                     /locus_tag="PWY67_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057465.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="PWY67_001168"
     CDS             complement(682..1257)
                     /locus_tag="PWY67_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2057466.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="PWY67_001169"
     CDS             complement(1989..2570)
                     /locus_tag="PWY67_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2057467.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="PWY67_001170"
     CDS             complement(2753..3379)
                     /locus_tag="PWY67_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2057468.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="PWY67_001171"
     CDS             complement(3401..4732)
                     /locus_tag="PWY67_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL2057469.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="PWY67_001172"
     CDS             complement(4887..5288)
                     /locus_tag="PWY67_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2057470.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="PWY67_001173"
     CDS             complement(5374..5580)
                     /locus_tag="PWY67_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057471.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="PWY67_001174"
     CDS             complement(5675..7177)
                     /locus_tag="PWY67_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL2057472.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="PWY67_001175"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="PWY67_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2057473.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="PWY67_001176"
     CDS             complement(7748..8365)
                     /locus_tag="PWY67_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL2057474.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="PWY67_001177"
     CDS             8586..9959
                     /locus_tag="PWY67_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2057475.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="PWY67_001178"
     CDS             10034..11146
                     /locus_tag="PWY67_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL2057476.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="PWY67_001179"
     CDS             complement(11218..12855)
                     /locus_tag="PWY67_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL2057477.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..13631)
                     /locus_tag="PWY67_001180"
     CDS             complement(13023..13631)
                     /locus_tag="PWY67_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2057478.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14015..15166)
                     /gene="queG"
                     /locus_tag="PWY67_001181"
     CDS             complement(14015..15166)
                     /gene="queG"
                     /locus_tag="PWY67_001181"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL2057479.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15185..15937)
                     /locus_tag="PWY67_001182"
     CDS             complement(15185..15937)
                     /locus_tag="PWY67_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057480.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16078..17028)
                     /locus_tag="PWY67_001183"
     CDS             complement(16078..17028)
                     /locus_tag="PWY67_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2057481.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17029..17868)
                     /locus_tag="PWY67_001184"
     CDS             complement(17029..17868)
                     /locus_tag="PWY67_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2057482.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17865..18611)
                     /locus_tag="PWY67_001185"
     CDS             complement(17865..18611)
                     /locus_tag="PWY67_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2057483.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18849..20219
                     /locus_tag="PWY67_001186"
     CDS             18849..20219
                     /locus_tag="PWY67_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2057484.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20274..21170)
                     /locus_tag="PWY67_001187"
     CDS             complement(20274..21170)
                     /locus_tag="PWY67_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2057485.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21284..21454)
                     /locus_tag="PWY67_001188"
     CDS             complement(21284..21454)
                     /locus_tag="PWY67_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057486.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(21451..22128)
                     /locus_tag="PWY67_001189"
     CDS             complement(21451..22128)
                     /locus_tag="PWY67_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2057487.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22219..23124)
                     /locus_tag="PWY67_001190"
     CDS             complement(22219..23124)
                     /locus_tag="PWY67_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2057488.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23299..24753
                     /locus_tag="PWY67_001191"
     CDS             23299..24753
                     /locus_tag="PWY67_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2057489.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            24769..25800
                     /locus_tag="PWY67_001192"
     CDS             24769..25800
                     /locus_tag="PWY67_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2057490.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25807..26994
                     /locus_tag="PWY67_001193"
     CDS             25807..26994
                     /locus_tag="PWY67_001193"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL2057491.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27150..28532
                     /locus_tag="PWY67_001194"
     CDS             27150..28532
                     /locus_tag="PWY67_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2057492.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28504..28890
                     /locus_tag="PWY67_001195"
     CDS             28504..28890
                     /locus_tag="PWY67_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057493.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29090..29758)
                     /locus_tag="PWY67_001196"
     CDS             complement(29090..29758)
                     /locus_tag="PWY67_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL2057494.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8404 a         6141 c         5483 g         9955 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    13621 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    13681 ttatcattta gaaatatttc ataggtaaaa gaaacatgac atttaatgac aaaataacga
    13741 attatttaat atttgtaaca tttaaacaaa tatttttttg atacaaaacc ataaaagcct
    13801 tgttgtataa gcaaagtaat cgtttttcta tgttttttta tttttgaatg aaaaacgcag
    13861 attctgtaac attcactgca ataatccata aaaaaaagca aaaaaatttt tgatttttat
    13921 caaattctct taaaaaaatg taaaaaatac aaaaaaacca gtaattgtta caacaattac
    13981 tggacaaaaa ctttgaataa tgttaactat ttttttattt taactttctc cgcaataatt
    14041 tctgggcatg ttccaattcg tttataacga cttcttctgt ttctttagct aatatttcat
    14101 ctaatacatc aagccatttt tccggttctt ttgttcctag acgtccaata gcccaagctg
    14161 ctgttcctct gatgacaggg cggacatctg ttaaacattc tgaaatctca ggcaatgccg
    14221 ttcgatcacc tagattagct aaagcaatca atgcgtttct ctgcaaaggt ttcttcccac
    14281 gccatgaacc agctaaatgc ccaaattgct gtttgaagtc tttattagac atcgaaagca
    14341 ttggctttaa ttttggatat acttcgtcta cttttggttc catctcttca tgaaaatgaa
    14401 aatcttttcc ttgattgtaa gggcagacta actggcagat atcacatcca taaatgacat
    14461 ttcgcatttt tttacgatat tcttccggca tcatcccttt tgtctgagtt tgataagaca
    14521 aacatcgctg ggcattcatt cttccgtcac caagcaatgc ttttgtcgga caagcagtca
    14581 cgcagcgcat acagtcaccg caaccaaatt ctcctggttt atcaggctca aatacaatat
    14641 tagtaacgat ctcaccaaga tagacaaatg aaccgaactc ttcagtgatc aacaatccgt
    14701 ttcgtccaat aaatcctaaa ccagctcgct gtgctacagc gacatcgatc agttctccag
    14761 tatctacttg cggagctaga cgccattcct cttctttttg ccatttttct gctcttgttt
    14821 ggatgaattc gatcaatcgg ttcaaacggt ctctgagtat atcatggtaa tcgatccccc
    14881 aagaagcacg agcaaactgc ccgcgttttt catccctagg cacttcttca tggatcctag
    14941 taggatacgc aagtgcaatg gaaatgatcg atttcggctg atcaaatgtc atttctggat
    15001 aaagacgctc atcgatattt ggatgttcaa agccagaagt atggccagct gctttttgtt
    15061 cttccaacga ttcttttaat gaatcaaaag gttccgccgt ggtgaacccg attttgtcaa
    15121 ttcctaatcg tttgctttct tgtatgattt cttcttttaa tgtcatggtc ttcctcgctt
    15181 gctgttaacg gctgtataac ccataatata gtttttcttt tttcagctgg acaaactgga
    15241 tctgctgatc ggcaatcttc ttttcttcca tccatttttt tcttgcagca aatacttccc
    15301 aataatttgt tgaaattaat tttcttttgt tatctaatgc attaaaaaaa ttcaatgaaa
    15361 ctggttttgt acttaacagt tcatggagcc ttacctcttc ccatggcgaa aactcagaca
    15421 gatcgcggga atacaatact tcttcataga gatttttttg ctgtgtgtac gtgaatgttt
    15481 gcgggatttt cccaggccta gcaatttcta ctagacgatt ataaaacata ttcattaatt
    15541 tgctgttggc aataaattga cttttataat aggccgactg gatctttttg attactcctt
    15601 ctgctttgat gactgaaaat aatgcaggcc atgctttgac ggttatttcg tcgttttgtt
    15661 taactaatac gccaggtgtg aagccgactg gagaaaggaa cacaaaataa ccgataccgg
    15721 tattttcatc taaaggctga aattcatcaa acaaccattt gcctatgtac agctcttcat
    15781 ctggttctgt tgtctcattt tgatttgcta tgaataattt agctaattct gcttcaagaa
    15841 attgaaggtc ttgatccttg tcggtatttt cctgctggct gatcaatcga aagacttctt
    15901 ctttaatcgt atatttatct ttacgtgtaa aattcatagc cacaccttcc ttccttactc
    15961 taactataac ggaatttctt caaaaaaggt atctgtacga cgaaaaacag cataattttt
    16021 tttcatatct cttataaaaa atgcaaggtc tgctcctcaa gctagacctt gcacttttca
    16081 gctttctttt gtttgtgaca aaccttcatg gatcgtttca atattccatt tcatcatctg
    16141 atagtaagaa tctccttgtt ctccttcttt tgctatggaa tcagtgaaca gtttatcata
    16201 gattttcaaa cccgtttctt ttgctacccg ttccatgctt cttgagtcta cactggtttc
    16261 cacgaataaa accggtactt cttttgctct tatctgatca atgatcgctt tcatttgatc
    16321 aggcgtacct tgactttctg tattgatctc ccatatataa gctgctggca aatcatatgc
    16381 tcgtgaaaaa tacttgaatg ccccttcact tgttactaat aattttttgt tctcttcgat
    16441 agaagcaaaa ctttccttcg cttgtgtatc taattctttt agtttttcca catatgcttc
    16501 tgcatttttt ttatagagtg tcgcattttc tgcatctttt tcagagaata tacgtgcgat
    16561 ttcctccaca tattttattc cgttagatag gtctagccaa gcatggggat ctgcttttgt
    16621 atgttcttca ccgctagtta aataaagagg ttctacattt ttgctaactg caaaataatc
    16681 ttttccttct tcttttttag ccgtttccat caagttatcg aaccagctgt tacctgtttc
    16741 aagattcaaa ccgttgtata aaataacatc tgcatcgctt gcctttttga tgtcttctgg
    16801 tagtacttca tattcatgcg gatctgttcc gactggtacg atactgtgga tatctatttg
    16861 atctgttccg acctcttttg ccatgtccgc caagatcgaa ttggtagcca cgactgacaa
    16921 cttttcttta cttccttcat ccgcttggtc agaagcctgc tttccgcaac cggctaatac
    16981 caagaaacta actgccaaga ttaaaaagag tgattttttt gttttcatct atctgcttcc
    17041 tttctgaaat aaaattcctt ttgttggaga aaataaaaaa gcaaggataa aaaatgcggt
    17101 acatgctaac acgatcgtag ctcctgatgg ccaattgtaa ctataactga aaaacacacc
    17161 gacaactgca cttagcatac cgattcctgc agctgtaatg atcattttca gtaaatggtt
    17221 cgttagcaag taagccgtgg cggctggtgt gatcaacatg gcaatcacca agattgttcc
    17281 tactgtctgt aaactgacta cagccactaa tgtcaaaaag aacatcaaaa gataatggat
    17341 cagcgatgtg ttcagcccat aagcttttgc cattgtcgga tcgaatgatg tgatcttcaa
    17401 ttctttataa aagaaaaaga caaatatcaa gacgattcca ctaacgagtg ctgtcagcaa
    17461 caaatcgctt tccctaacag ctaagacatt cccaaataaa atatgataca aatctgtcgc
    17521 actctgggca tatgaaatca agataatccc taatgcaaaa aaggagctaa agacaattcc
    17581 aatcgctgta tcatttttca atcggctccg ctgagtcacg aaaccaatca ctgctgctgt
    17641 tagcatccca aaagcagtgg ctccaaagat gtaactaaaa ccaaacatat aagaaatggc
    17701 tacacctgga agaactgcgt gagagatagc gtctcccatc aaagacatcc ctcgtaaaat
    17761 aataaaagaa ccgatgactc cagaagcaag tcctacaagc atggaagtga ccaaagcgta
    17821 ttgcaggaat tgataattca ttaatccatc aataaaggat tggatcattt tttctctcct
    17881 ccttttacta aatgtctgat gatctcacca taggccaatt gaaggttctt cgatgtaaag
    17941 acttcttgta ctggaccgga agcgatcagc tgtttattta aaagaataac ctcatcgaaa
    18001 tattcttcta ctttatgaag atcatgatgg acgatcaaaa tcgtttttcc tgaattcttc
    18061 aattcctgca agatgtcaaa gatctttcgt tcacttaacg catcaatccc tacgaatggt
    18121 tcatctaaaa agatccattc tgctccttgg gctagagctc ttgcaataaa aactctctga
    18181 agctgtccac ccgatagttc gctgatctgt cttttggcat attcttccat ccctactttt
    18241 ttcaaagctt gtcttgcacg ttctttttct tttttcccag gtctttgtcc aattcgtaaa
    18301 gatggatatg ttcctaaaag tacaacgcct aatacatcaa ttggaaagga aagatccaat
    18361 tcacttcgct gttccacgta ggctattttt ttacggactg agtttaccgg ctgtccagaa
    18421 aaggtgactt taccagtagt agctggtatc aactctagca aagatttcat aaatgttgat
    18481 tttcctgcac cgtttggtcc gataataccg gtaatttttt gtggatttat cgtcaagttg
    18541 atagttttca aaacagtttt cccttgataa gcggctgaaa ggtcttgaat tacaatggcc
    18601 gtacgttcca aatcttacac tcctttttag gtttacctaa ctttttattt aaatatagtt
    18661 taactaaaat ggaaagcatt ttcaagtaga atgatgaaaa agtttttttc gaattttcct
    18721 cgcattttat gaaaattttc tgtagaatca catcaagagt tcaataaggc taacctaata
    18781 atgaaaaaca tgtgttgaaa cgtttgatta ttttttcttg ttgactcaac aacgaaagga
    18841 gtttttgtat ggaagaagaa aagttaactc gaagtcttag tgcccgtcat atccaaatga
    18901 tcgcactagg tggaacgatc ggtgtcggtc tgtttatggg tgcctcctca accatacgtt
    18961 ggacagggcc ctcagtgatg ggagcctatg cgattgcagg attctttttg tatttgatta
    19021 tgcgtgcttt aggcgaaatg ctttacgttg atccttcgac tggttctttt gcaaattacg
    19081 cgagcgagta catccatcct gtggctggct acttgaccgc ttggagtaat atttttcaat
    19141 atattgtcgt aggaataagt gaagtgattg ctgtaggatc ttatatgaat tattggtggc
    19201 ctgatttgcc ggcaattatt cccggaatca ttgtcgtctt gtttcttatg ctggcaaatc
    19261 ttatttctgt caaggcgttt ggcgaattag aattttggtt ttcgatcatc aaagtcataa
    19321 cgatcatttt gatgatcatt gcaggtcttg gggtaatctt atttggattt ggtaatcatg
    19381 gtcaggcagt agggatttca aatctttgga aaaatggtgg attcttcact ggaggcgtca
    19441 aaggattttt ctttgcatta tcgatcgttg ttgcttccta tcaaggaatc gaactgatcg
    19501 gaatgactgc tggtgaagca gagaatccta aaaagacgat cattgaagct gtccagtcca
    19561 ctatcggtcg aatcttgatt ttttatattg gtgctatttt tatcattgtg agtatctatc
    19621 cgtggaatca attgagtgag attggttcac cttttgtaca aactttttct aaagtcggca
    19681 ttacatttgc tgcaggactg attaattttg tcgtgattac agcggcatta tcaggttgca
    19741 attcagggat tttcagcgca agtcgtatgg tctatacact tgccataaat ggaaagatgt
    19801 ctaaaaaatt tctgaaacta tctcgtcacg gtgcgccttt ttatcctgtc gtagcaatct
    19861 caggtggaat cttaattggt ctgatcttaa attatcttct accaatgttc atcaaccagt
    19921 cagaaaatat tttcgttttc gtatacagct caagtatttt gcctggtatg gttccttggg
    19981 tagtgatttt gatcagcgag attcgcttca aaaagaagca cgcagataaa atgcatgatc
    20041 atccttttaa aatgccacta tctccttata cgaattatgt gacacttgct tttttagcta
    20101 tcgtattgat tttcatgttt atcaatcctg aaacgagaat ctcattgatt attggactcg
    20161 ttttcttgct ttacatgacg atccattact ttgtacgcga aaaaagaacc ggaaaataaa
    20221 aaaagaacga agcttcacct tagtggaagc ttcgttcttt tttattgctt ttatcaattc
    20281 aattttagcg tctcaccggg agtcaggaaa taattctcca tgctcatccc attcagcgac
    20341 aagatcgttt ctactggtac gccagttcgt tcggctattt gatttggacc ttctccttcc
    20401 aaaactacgg ttgtcgcagc ctgtgaagtc gcgctgttgg tattagtttc agaggaatct
    20461 gttgtgtctt gtgttgttcg agcttgcatt tcagaagctt ctaccgactc tgctgtctca
    20521 gaatcagaag atgttgtgtc ttgtgtgctt gttgactctg cttgaagaga ggcgctagtc
    20581 tgcaaataaa tatcggaaat cttagcgaca tgattcgtat aattagccga taattgattg
    20641 atcaaagtat ctaagtctac accgtcttta ttttctgctg cctgatacgc tgcacgtaat
    20701 tttctgatcc cttcgtcgga gattgcttga agctcacgtg ctttttggtt agcaactgct
    20761 gataaaccag ctattccctc ttgatttccc tgaatttctg tctggtattc ttcaatcaat
    20821 gcaggcgtct tttcttcgat ctttttcgaa taatcattta cgattgcttg aagttgctct
    20881 ggcaaaccat ttgctgtttt ttcttcagaa gtttgtgtct cttttgtttt agttgatgac
    20941 tcacttgttt gtttcgtctc tgttttcttt gtcgttgttt ccgttatggt cgtacttttc
    21001 gcttctgacg aattttttcc atgaaaatat agacccgcaa ctcctcctgc tatgatcaaa
    21061 aacacggcaa ctgctgcccc aatcatgatc atccgcttac gagctgcctg tttttgctgt
    21121 cttcttcggg tcatccgaac attttctccc cactcatttt catcatacat ttttttcacc
    21181 accaattttt atgtataata gcgttatcaa acgcttcata ttcttattgt agcaaataat
    21241 tgtcgctgtg cacattttta tctttgttat ttataagaaa atttcaccag ttgagatgat
    21301 tccaagattg tccgtcgatt tctccttgca gctgcattgt tcgttcgtct atctcagcca
    21361 tcagtttctt tatttcatat aagagcgcac ggtcttgata ttctgatttt tcgcttaatt
    21421 catctaattg ttggatcagc caatcatttg tcataatgaa gttcctttca tttcttgcag
    21481 tgtaaaggtc aagtcttcga tctgctcttt tgcctcagtc agtgcttcta cgatatatgc
    21541 ttgtttttca gattcttctt cctgcatttt ctgattttcc tgtactcttt gaataaacca
    21601 gtcaatccgc tgagtcagcg atggtttgcc ttccaatgag agatactgct gataagcaat
    21661 tagcttctcc caacgttttt cctgggataa atacataaat tgtgctaaaa cgaatggcgg
    21721 aagataggtc atatggaatt tatttgcgat tcgctgatac ggcctcaaaa attcggaaat
    21781 cgtatattct tcttttcctc cagcttggta ttctttttcc gccacacctg ttgtcaatac
    21841 gattcctagt tctttccctt ttaatacatg tgctgctcgt tccaagacat tatcttgcca
    21901 aatttttaaa tgagcaggag aactatacca gtaaaacgga aactggaaaa tgatccgatc
    21961 atgctgggcc aagagctgta gttcagtctc tatatctatt tttccgtctg gataacttga
    22021 ttccaaatga tgccaagtag ctggtaaacc agcagaagct tctttgaaaa attgctgatt
    22081 taaagattct tcgatcgttg gatggctaat gatgataagt gtacgcatcc cttttcctcc
    22141 tttttagtct tcgctttaca gtgtagcata accgccacaa gtttcttttg ccgatgcttg
    22201 tcattttaac cagactaatc aggttgtcca agaaactgcg gtttgttttt ccaactgttt
    22261 tttcaaggta tccaaagcaa tattcttaaa attttcgttt ttcatacgtt tacgatagtt
    22321 cgtagccgtt tctccggtac tctttttgaa cattcggcta aatacgacta gactattgaa
    22381 ccctacttga taggcaatac tttccacgga ttcatccgtc agagaaagaa gaaacgcccc
    22441 tcgattgatc cgtaccccga tcaaatattc cttgatactg gtacccatat gatttttgaa
    22501 tactcgcgac aaatggctcc gattgaccgc taaatgctgg gcgatttcct caacagacaa
    22561 tttttcagca taatgtgcct cgatgtattt gactgtttca acagctaaag aggaatgaac
    22621 cttttgtaca ccaccgctga cctttccgcc atcttttaaa aggactgata aaaaacggta
    22681 ggttaatgct gttaactgta attcattttc caaggtttct tccgtatctt tcatgcattc
    22741 taaaataatg tcgatatatt tttgtatgtc agaagagacc atcgtgtatt ggtcttctct
    22801 gaaaagcgac tgccgaatga tttcttctgc agcactccca ccaaatgaaa gccagcaata
    22861 catccacgga tcttctccat ctgataagta aaacgtcgag tctcctggac gaatcaagaa
    22921 cagatcacct tttttcaatt ggaattgttg atttttcaca tgataagatc ctttgccttc
    22981 catcacgaca tgcaggatgt aatcattgcg aatagcagga ccaaaagaat gaaacggcat
    23041 cgttttagag aaaccacaaa aatcaaaata caattctttt tctacttgtt gatgttcctt
    23101 ttttaaaaaa atcgattgct ccacgtctat ccctccttac tcctttagta taatgcaaca
    23161 aaaagttaaa taaaagtcac agaaatatat taaaaaggaa gcgatttctt gttaagttta
    23221 agtcattaaa agatctttgt tattcgttgt aaccggttta cttagtatta tatattgaac
    23281 aggagaggaa tacagattat gaaaagcaaa atgatccaac gtgtgtccta cgctttaggc
    23341 gcttttggac acgacacatt ctatgttaca ttaagcactt attttatggt attcgtgaca
    23401 agccagatgt tcgcaggaac agataaggca acgaatgcaa aaatgattgc tacagttaca
    23461 agcttagttg taggtatccg cttagttgaa attatttttg atcctttgat tggtggcatc
    23521 atcgataata ctcgtacaaa atacggtaaa ttcaaaccat ggttagttat cggcggcgtt
    23581 gttagttccg tactcttggt agcaatcttt accaatttct ttggcttagc tactcataat
    23641 acgactttat ttatcatatt atttgcaatc gttttcatct tattggatag tttttattca
    23701 tttaaggata tcgctttttg gtcaatgatt ccagctttga gtacggaaac aaaggaacgt
    23761 gaaaaatttg ctacgtttgc tcgtttcggc tcttctctag gtgcaaatgg tactactcta
    23821 gtcgttgtcc ctatcgttac tttctttacc tatcttgtta ctcataaaca tattgaaggc
    23881 gcttcaggct ggttctggtt cggtgtgatt gctgctgtta tttcaggcgg tactgcgatt
    23941 atcactgcct tgggcacaaa agaagtcgat actgcgatcc gtcagcaaaa tgaaaaagct
    24001 gggatcaaag atgtgttcaa agcagttgct caaaatgatc agttgatgtg gcttgccctt
    24061 gcttatatcg cttatgcgat tgccaatgta gcaacaactg ccgtattatt ctacttcttc
    24121 aaatatgtac ttggccaacc aactgaattt tggatcgttg gtctagttgc tgccttactt
    24181 ggtttagtag ctgttccact attcccaatg cttacacgcg tcatctctcg tcgctacgtt
    24241 taccttggag ggatcgcaat gatgatttct tcttactttt tctttacgat cgcaggttca
    24301 agtttgccaa tcgttatcat cgggctagta ttgttctact tccctcaaca attgattttc
    24361 ttatccgctt tgatgacgat cacagactct gttgaatatg gtcaatggaa aaatggtatc
    24421 cgtaacgaag ctgttactct atcaattcgc cctttactag ataaaatcgc tggtgctttg
    24481 tctaacggga tcgtagggtt tgtcgctatc gcttctggga tgactggtaa tgctactgct
    24541 gctgatatca ctgctcatgg cgtaacgact ttcaaagctt atgcattcta tgctcctgct
    24601 gctttgatga ttttctccgc tattattttt gcttggaaga ttacattgac agagaaaaaa
    24661 catgcacaaa tcgttgagga attggaaaac aaattggctc cagctgatac aatggaagat
    24721 gaacttcacg atgcagtagt tccttcccat taagaaagga tcttgaaaat gaagattaca
    24781 gaaaaagatt ttggccaagg ttatcacttg ataaccttgg agaacaaaaa caagctagca
    24841 ctttctatct ctgatttagg tgctagaatc gtcagtttaa aatcaaatga tcgagaatta
    24901 gttttgggat ttgacaccgc tgaagaatat atcgaaaaag atccgtatat cggtgcttct
    24961 atcggtcgca ccgcagggag aatcgaaaat ggccgctttt ctctcaatgg aaaaacttac
    25021 caattagcca cagatccaaa aactggacat aatctgcatg gtggtgctcc tggatttgaa
    25081 ttaaaaaaat ggtcttatgt cattttgaat ggcgaaaatg aagcttctgt catttttact
    25141 actacaagtc ccgacggaga acatggtttt ccaggtacaa tggatgtgga aatccgctat
    25201 actttgacaa aagataatat ctggcgagtg acctctcgcg gcaccagtga ccaagatact
    25261 ttattcaatc cgacaaatca tgtttacttc aacttgacgg gagatgcttc tcagtcaatc
    25321 gatcaacatg agctttggct caatagcgaa gcttatgctc ctttgcgaac agatagtatt
    25381 cctatagggg taaaagaaaa tgctgcagga agtgctttcg attttcaaat acctaaaaaa
    25441 ttagcgtccg tttttgctag tgatttggat caaaaaaatc ttgttgacgg aatcgatcat
    25501 ccatttttct taaaagaaac cggcttgggt aaagaagccg ctcgcttgac tagccccgat
    25561 aaaagaatcc aagtaggtat tgctacagat gcttctagtg tggttatttt cactgcaaat
    25621 ttcggcacag aaacaccaga aatgcgcaac cgaaaactgg cacatcacgg tggcatcaca
    25681 tttgagactc aaacagctcc aggtgcagag cgcttctcag catttggttc gattcattta
    25741 aaagctggat cagtatttga aacagtgaca gaattcaaaa tcaaaacaag aaaggaataa
    25801 ctcatcatgg aaatcaaaag aagcttaaac acaaaatttg ccgaaacatt cggacctaaa
    25861 aaaactacac aatacttctc accaggacgc atcaatttga ttggtgaaca cacagattac
    25921 aatggaggcc atgtcttccc cgcttccatc acttatggaa cgcaaggtgt agcagcacct
    25981 cgagacgata ataaaatatt agtttattcc acaaactttg acgatatcgg tgtcattagt
    26041 tttactttag atgagctaga atatgataaa aaagcaaact gggcaaatta cgtaaaagga
    26101 atggtcctta aattaaaaga agctggacac tcaatcgatc atgggtttga gttgttagtg
    26161 gaaggaacaa tccctaatgg tgcaggactt tccagtagcg cttcattaga attattagta
    26221 ggtgttgtat tagaagatct gtttgattta aaaacagatc gcttggaact agtccaaaca
    26281 ggtaaaaaag tagaaaatga attcatcggt gtcaattcag gtatcatgga tcagtttgcc
    26341 atcggcttcg gtgagctaga tcatgcaatc ttattagata caaatacgct gaaatacgaa
    26401 atggttccag tgaaactaga tggttatgca atcgtcatca tgaatacgaa taagcggcgg
    26461 gaattggcag attcaaaata caatgaacgc cgtagcgaat gcgaagaagc cttggcacgt
    26521 ttgcaaaaaa ctttgcctat ctcttcttta ggagacttag atgaagaaga atttgaatca
    26581 actattgacc aaatcggcga tgatacattg atacgcagag ctcgccatgc cgtttctgaa
    26641 aatcagcgga ctttgaaggc aaaagctgaa ttagaagctg ggaacttaga agcatttggt
    26701 caactgttaa atgattctca ccactctctt cgctatgatt atgaagtaac tgggatcgaa
    26761 ttagatacac tagtagatgc tgctcaaaaa caagaaggtg tcttaggtgc acgaatgaca
    26821 ggagctggat ttggtggctg tgcgattgct cttgtcaaag aagaaaatat acctgccttc
    26881 aaaaacaatg tctatgatga atacatgaaa gttgtcggct atgctccgga attttatgtt
    26941 gcacacattg gaaatgggac aacaaagatc gatccagaag ctgttcatgt ttgataaaaa
    27001 aatcaacgat ctcagctaaa aacaaataga taaaaataac ctctcagcaa tttccaaatg
    27061 tgagcctttt gctcgtattt gaaaattgct gttttttatt gacacggaaa gagaacgtgt
    27121 gttctaattg tgttgaagag gtgattgtta tgtgggcgaa tcaaaatccg gtatttgttt
    27181 atgaaaaaga accttcaaga gatattttat gtattgactg caaatctttt tacgcctccg
    27241 tagagtgcgt tgaaaaggat cttgatcccc ttacaactaa attagtagtc atgtcctatc
    27301 cgtccgattc gacagaaaca cggggtagcg gattaatctt ggcttccagt cctgcagcaa
    27361 aaaaagctta cggcatcacc aatatcagcc gtgccagaga tcttcccttc ccctatccaa
    27421 gtgatttata tattgttgcc ccaaggatgg cctattatat ggaaaagaac agagaaatca
    27481 attctatcta taaaaaatat gcagacgaag cgaaccatca tgtctattcc gtcgatgaaa
    27541 gcttccttga tgtaacaaat agcatggcac tctttggtaa aacaaatgcg tacgacatgg
    27601 ctaaactgat ccataaagat gtgtttgagc agaccggaat ctacacgact ataggaattg
    27661 gcgataaccc gctacttgct aaactagcct tagacaatgg ctcaaaagac ggaaagcaaa
    27721 tgtttgctga atggcgttat gaagatgtac cagaaaagtt atggaaaatc tcagatatca
    27781 ctgacttttg gggaatcggc aaacgaacag caaaacgtct gaaacaaatg ggtatacatt
    27841 ctgtttatga tttagctcac gcggattatt atcacttgaa aagcaagcta ggggtcatcg
    27901 gtactcagct ttatgcccat gcttggggca tcgatcgaag ttttttggga caagaatata
    27961 caccaaaatc aaaaagtatc ggaaatagtc aaatcctaga tagagattat acaagacgag
    28021 aagaaatcga gatcgttatc aaagaaatgg ccgaccaagt cggcactcgc ttgagacgat
    28081 ctgcagcaaa aacacaagta gtcagcttat gggtaggata ctctttaggc tatcttgatc
    28141 caaatggcaa gaccgggttt caccagcaaa tgaaaatcga agcgacaaat gctagcagag
    28201 atctagcaga tgctttattg accattttcc accagcatta ccactaccag gatattcggc
    28261 gtgtaggcgt gaactgttcc gacctcgtat acgcgacagg tttacagctt aacctttttg
    28321 aagatcctga aaagcagata aatgaagcga aagttgattt tgttgtcgat acgattagaa
    28381 aaaaatacgg ctttcaatca atcatccatg cgcatagtct cttgcaagga ggacgcgcca
    28441 tcgcacgtag ttctctcgtt ggcgggcatg caggtggtat gagtggatta gaaggtggaa
    28501 aagatgagac ggacagcaaa aaaattcatt gactataacg agtatcgaga tcggccattt
    28561 ggattaaaat gggggactgc ttttgcgatg gaagaactag tcaacggtat caaaaaaaat
    28621 gaagaagaag cacgcaaaca gcctcttcct aaacaacaaa tgacattgca agaaatcgat
    28681 agtatactga cagaagcctt tttatctcgt aaagcagtaa tcattcagct caatttaaaa
    28741 gatgaatttg gaagactctt ggatgaggtc gaagggcact tcattggtga agcctatgaa
    28801 gattacttta tgattgacca tcagccgata ttgtgggaag acgtacggta tattgagtta
    28861 aaggaaacaa aaaaatggtt tgtgacatga gaggttgtga cagaagtgat caacttcaaa
    28921 aaataagaaa ggatttttcg aaaattgctt cttaaatttt tgtggaattt tggcttattt
    28981 ttcgaagaag caacttctgg aacaacgtat attagatatc agaggttgtg aaaaagtcgc
    29041 ttagttttga gtaacaagcc aaggcgctga tttcacaacc tctgtctatc tatccatcta
    29101 ttcggtaaga ttcatgcatg acgtcttctt tatttagtat gtagagccct ttttcttctg
    29161 accattcaac ataaatcagc tgttttacat ctgcatagcc ttctttcttc aagttctcca
    29221 taagccatgc ttcagtcaat tgattggact gaagcacttt atgctgaatt tgcccttcat
    29281 ctacaagcag gatcgataaa gctttgtctt catctgattt tttcaataca ctcatttgac
    29341 cagcattctc taggataaca tgtttggcat tttcaagaga aaagcattct tgctgtctta
    29401 agattgctcg caactgctcc atctcgatat ggttatttgt caactctgtc atgttgatga
    29461 cgccgtcttt gattagaaca gatggctcgc ctttgaccca acgattgacc ttttcatttt
    29521 tttggacgat acgttcgata ccataaatca ttactgccca aagcgcaatg gcaaatagga
    29581 catgccctac atggacattg tcatcatagg ttgattcttc taagatccca cctaaaacaa
    29641 gcgtatagac taaatcaaac ggtgtgattt ctgacatgga tttcttccca agcaaacgta
    29701 ccactaataa taaaccgatc aacccgaaca ccagtttcat tgtaattgaa aaaatcattt
    29761 tttctccccc taactatctg ttaattaaat tatatatgct ttctcacgaa cacgcatgga
    29821 atatacaagg aagaaatgta tccttttact aaaaaaagga ctgtgacact gtagtgaccc
    29881 ccaaaagtta gacttttttg gagtggagaa atccactcct tttttttatg ctctaaaatg
    29941 atatttttac acaaccgtta cggttgtttt acgaaattgg acg
//
LOCUS       DANKAZ010000029        28682 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000029 DANKAZ010000000
VERSION     DANKAZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(132..1439)
                     /locus_tag="PWY67_001197"
     CDS             complement(132..1439)
                     /locus_tag="PWY67_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL2057495.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1442..2023)
                     /locus_tag="PWY67_001198"
     CDS             complement(1442..2023)
                     /locus_tag="PWY67_001198"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL2057496.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2139..2236)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2595..3887)
                     /locus_tag="PWY67_001199"
     CDS             complement(2595..3887)
                     /locus_tag="PWY67_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2057497.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(3884..4507)
                     /locus_tag="PWY67_001200"
     CDS             complement(3884..4507)
                     /locus_tag="PWY67_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057498.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4510..5346)
                     /locus_tag="PWY67_001201"
     CDS             complement(4510..5346)
                     /locus_tag="PWY67_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2057499.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5363..6250)
                     /locus_tag="PWY67_001202"
     CDS             complement(5363..6250)
                     /locus_tag="PWY67_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2057500.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6258..7493)
                     /locus_tag="PWY67_001203"
                     /pseudo
     CDS             complement(6258..7493)
                     /locus_tag="PWY67_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(8176..8859)
                     /locus_tag="PWY67_001204"
     CDS             complement(8176..8859)
                     /locus_tag="PWY67_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2057501.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(8877..9494)
                     /locus_tag="PWY67_001205"
     CDS             complement(8877..9494)
                     /locus_tag="PWY67_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2057502.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9504..10328)
                     /locus_tag="PWY67_001206"
     CDS             complement(9504..10328)
                     /locus_tag="PWY67_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL2057503.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10321..10881)
                     /locus_tag="PWY67_001207"
     CDS             complement(10321..10881)
                     /locus_tag="PWY67_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057504.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11055..12011
                     /locus_tag="PWY67_001208"
     CDS             11055..12011
                     /locus_tag="PWY67_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2057505.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(12316..13272)
                     /locus_tag="PWY67_001209"
     CDS             complement(12316..13272)
                     /locus_tag="PWY67_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL2057506.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            13376..13978
                     /locus_tag="PWY67_001210"
     CDS             13376..13978
                     /locus_tag="PWY67_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2057507.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(14066..15565)
                     /locus_tag="PWY67_001211"
     CDS             complement(14066..15565)
                     /locus_tag="PWY67_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL2057508.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(15581..16105)
                     /locus_tag="PWY67_001212"
     CDS             complement(15581..16105)
                     /locus_tag="PWY67_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL2057509.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            16403..17152
                     /locus_tag="PWY67_001213"
     CDS             16403..17152
                     /locus_tag="PWY67_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2057510.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            17143..17928
                     /locus_tag="PWY67_001214"
     CDS             17143..17928
                     /locus_tag="PWY67_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL2057511.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            17897..18613
                     /locus_tag="PWY67_001215"
     CDS             17897..18613
                     /locus_tag="PWY67_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2057512.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(18715..19749)
                     /locus_tag="PWY67_001216"
     CDS             complement(18715..19749)
                     /locus_tag="PWY67_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL2057513.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(19753..20811)
                     /locus_tag="PWY67_001217"
     CDS             complement(19753..20811)
                     /locus_tag="PWY67_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL2057514.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(21031..22179)
                     /gene="nagA"
                     /locus_tag="PWY67_001218"
     CDS             complement(21031..22179)
                     /gene="nagA"
                     /locus_tag="PWY67_001218"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL2057515.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(22255..23091)
                     /locus_tag="PWY67_001219"
     CDS             complement(22255..23091)
                     /locus_tag="PWY67_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2057516.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(23275..24492)
                     /locus_tag="PWY67_001220"
     CDS             complement(23275..24492)
                     /locus_tag="PWY67_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2057517.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(24641..24820)
                     /locus_tag="PWY67_001221"
     CDS             complement(24641..24820)
                     /locus_tag="PWY67_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057518.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            24875..24958
                     /locus_tag="PWY67_001222"
     tRNA            24875..24958
                     /locus_tag="PWY67_001222"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24909..24911,aa:Leu,seq:gag)
     gene            complement(25198..26568)
                     /locus_tag="PWY67_001223"
     CDS             complement(25198..26568)
                     /locus_tag="PWY67_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057519.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(27282..27572)
                     /locus_tag="PWY67_001224"
     CDS             complement(27282..27572)
                     /locus_tag="PWY67_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057520.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(27780..28472)
                     /locus_tag="PWY67_001225"
     CDS             complement(27780..28472)
                     /locus_tag="PWY67_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2057521.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         8667 a         5969 c         4827 g         9219 t
ORIGIN      
        1 ataaacggtg ttcaatcagc tgaccctcgg tattagctca aacaaacggc aaaatacgag
       61 gaactatttt tccgtttgtt tctctaacaa tcaggtcaaa acgctgattt cacaaccttt
      121 cttttgctct attacttctt gaaggcttct tccaatattt cttcatcttt tttcaatcca
      181 ttaaagatca aattcaatat gatagccgta atgctactca ttacgatgcc atttccagtg
      241 aacatttgca atgtttgcgg catttgactg aataatgtcg gcgtaacatt gaaacccagc
      301 ccaaatccaa cagaaacagc aacaatcaat agatttttat cattgctgaa atcaacttcc
      361 aacagcatac ggattccttg gactgccacc atcccgaaca tgactaacat tcctccacca
      421 agtaccggct cagggatgat ttgtgctaac gcaccgattt tagggaataa tcctaaaaca
      481 atcaagaaga atgcagagaa atagatgggt tttcttgttt tgattcccga aagttgaacc
      541 aatccgacat tttgagaaaa gccagtataa gggaatgtat tgaaaatacc gcctaagata
      601 acggcaagtc cttctgcacg atatcccttt ttcaattctt cttctccaac atttttccca
      661 gtaatgtcac caagtgcaaa atatacacct gttgattcta ccatgctgac catggaaatg
      721 atgatcatta gtaaaatcga ccatacgtcg aaagttggtt taccaaaata aaagggttgt
      781 ggaacatgga acaacggcgc atctccaatc gcgctcagat caatcattcc catgaaagca
      841 gctaagatgc ttcctccgac taaaccaatc aataccgcga ttgaacgaat gaaccctttt
      901 gccagcattt gcacgcctac aattaggaga atcgtcacaa atgcaagtag cagatttgtt
      961 ttgtcaccaa aatcagctgc tgttgcatta cctccaccca ttttctcgat tgctacagga
     1021 atcaaagtca aaccaataac ggtgataacc gttcctgtta ctataggagg gaatagtttt
     1081 ttaattttag aaaagacacc agaaactaac acaacaaaga tcccagaagc gataatagat
     1141 ccgtagatag ctcctactcc ttcattggtt ccgatcaaga tcagtggagc tactgcttgg
     1201 atcgcacatc ctaaaacgac aggcaagcca attccgaaaa atcggttaac tgtcaattgc
     1261 aataatgtcg ctaatccaca cataaagata tcaattgaaa tcaaataagt catctgttct
     1321 tgattaaagc ctagtcctgt accgatcaaa agaggcaccg ctactgctcc tgcatacatc
     1381 gctagcagat gctgcagccc taaaaccgca gcttttccat tttgtgtttc tttttccacg
     1441 attatgcgtc ctcctcaaga aactcaactt gtccattcgt caaggaagca atacgcgcaa
     1501 gagaaacgac tctcaatccc atttcttcaa gtaactgacg tccatcttgg aaagactttt
     1561 cgatgacgat cccgattcct tctacttgcg ctccagcttg ctggcataat tcgatcaatc
     1621 cttttgccgc ttgtccgttt gccaagaaat cgtcgataat caatactttg tcttctgaag
     1681 ataaaaattt acgagaaata gaaatcgtac ttgtcacttg tttcgtaaaa gaataaacag
     1741 aagctgtcaa taattcttca tccatcgtca aactttttgc ttttctagcg aagatcattg
     1801 gaacatctaa ttctcttgca gcaaataaag ctggtgcgat cccagaagct tcaatggtca
     1861 caactttcgt gattccttgt tctttaaata cttctgcaaa ccgttttccc atttgttcca
     1921 tcaatacagg gtctacttgg tgagtgacga agctgtcgac ttttaatacg ccctcgccta
     1981 atatacgtcc gtctttttga atacgttcaa ctaattcttt cactttattt gctcctttat
     2041 catttattaa taccgaaata accgcttttg tgcaaaaacc tactatgttt ctgttaaaga
     2101 aaaacaccct ccactttatc ataagaaaag aagggtgaaa tagccaatct tttacttata
     2161 gtccagcatt tacggtactg gctagaaact gtcatccata ttatgacgat atataagtag
     2221 gtatgtaaaa ctaataatag tgtaaattca ttttcttgca actgttttgc acgtgaaggc
     2281 agtttgccat atttgaataa aaaatgttgt ttcaaatatt gaaaacagtc taacactact
     2341 ttctgaaact gacaacgtgg tttacttatt tttttgtaaa atctaatgat ttttaatgca
     2401 ttattccttt ttgttcgggt ttaaatagtt atttttattt tctcctatct aaaaaagcag
     2461 cccacataaa aagaaggcat gacaaaaagc atgtcatagc ccctttttcc gaataaatag
     2521 tgttcaaaag acagcctatc ggcaataagg caagatttta taaactacgg gaagtaattt
     2581 ccgtaaatct attcttattc ttttaagctg acaagctcgt caatcactac tgcttttcct
     2641 gaagcaatag aatgatttgc tgcgattcct gtcaaaatcg acaaaatacc atctacgtga
     2701 gttgctgccc gattccacgg atcttcttct ttttcttcac caaatagatc atctaataaa
     2761 cgcggatctc ctccgccatg tccgcccgct tcttgttcga cgattacctc atatggctca
     2821 ccgaacatcg gatagacatg gatggactga agaacggctg ctccttcatc ctttttatct
     2881 ccacctgcat tgatgtaaga cttttcgatc actttcattt cgatccgtcc tttgctgcca
     2941 ttgaaagaaa cattgaatcc ttcccacggc atatacgcgt ttagcgaata cgtcaaaatc
     3001 gcaccagttt catatttgac cgttacgcct agtgtatctt caatagagat atcatcggaa
     3061 aatacactgc ggtcacgaat atatccactg tctttttctg cttctaaata catttctcgc
     3121 aattcctcat tggctgacag gtctatagca aatggatcag ctttagctag ttcgcttcca
     3181 tagcatcgac tgtaaaatat ttctactccg cgtttctccg cattatgcat cccataaaag
     3241 tttaatccac cgaatgcaaa taccttttgg ggacggctgt tcaaccaaaa attcactaga
     3301 tcaaaatgat gggtagattt atgaacaaga agcccgccgc tattgtgctt atttcgatgc
     3361 catcttctga aataatctgc cccgtgttgc gtatttaaca gccattcgaa gtggatagaa
     3421 aacacatcac caatcacatc actttctatc agttccttta tcttcgtgtg atacggtgca
     3481 taacgataat taaaagtgac tcgcaggctt cttcccgtct gtttttggac cgttattatt
     3541 tcttgagctt tttcctcatc gatagtcaga ggtttttcgc aaataacatc atatcctttt
     3601 ttcattgcac gaatgatata atcatgatgc gttcgatcta ctgacgtaat aatgacttca
     3661 gccggccgta cgtcatccag tagttgctca aattgtgatg gcatacatgt ctttactttt
     3721 ttctgtccat acttgttcac aagtatccga tttgcataat ccatccttgt ttgagataca
     3781 tcacaaaaac cgactagttc acaagtttga tgatacgtag aggcaattgc ctcataaaaa
     3841 aaacgcgctc tcccaccaag tccgacttgt acatattttt tcttcattta aagtcattct
     3901 ccttttcttt taagcaaaat actgtgacaa atggcagccc tggatacagt aatgcagtga
     3961 gtaaaaaatc aggctgtata caccagaaca gtccatacaa aaggcataaa taccacagaa
     4021 accccttgac gttttttgac aacagccaga tattcctttt gatcaaataa acaagactat
     4081 ctgctttatc ttctttgcag atatcaatta gcgtactaaa aaaactatgg gatacaaaaa
     4141 gcaacagaca caatagaaaa ggaatcagat accaagctcc aatctttccc actaataaat
     4201 accaatcaac tgcaaaaata atgacaaaca gctgaaacaa cgtaatgatg aaacacatcc
     4261 gtttgatttt ccctttcctc caacatacct ttttttctgc caagtaatgg acagatagat
     4321 aataagaagg atacatcatc aaaaaataaa tcagatagac aacacgagca gacagcacta
     4381 atcctaaagt gaacaaacat cctagaacta aagcgaaaag agcattactg ataagactgg
     4441 ttaaaaaaat ccgacagaca tagtagttga gcttgtaaag aaacatctct tctattggct
     4501 ttttcatatt cagcccttca gaccgcttgt actgatcccc tggacaagat atttattgaa
     4561 gaataagaaa atcagaaaaa ccggtagcag cgacaacgta gacatcgcaa acatactcgc
     4621 gtaagtcgta ctagatccag cgtctgcaaa taatttgatg gcaatcgata ccgtgtaaga
     4681 tctcggcttg tttaaataaa tcaatggtcc catgaagtcg tcccatttcc aataaaactg
     4741 gatgatgatc gtcgtgatga tcgaaggttt cagcaaagga aaaataattt tggtaaagac
     4801 actatacttg ctgcatccgt caataatcgc tgattcatct aactctttgg ggatgctgac
     4861 catgaattgc atgatctggt aaatgaaaaa tgcttgtcca aaaaaatgtg gtaaaatcag
     4921 cggcacatac gtcccaacca aattcagccg attgtaaata ataaactgcg gaatcaaaat
     4981 cacttgtgca ggaatcatca tcgttactaa catgacgacg aaccagaatc tttttccttt
     5041 gaaatcgatt ctcgataatg cgtaagcaat acaagtcgag ctcagaactg tccctatcgt
     5101 cgaaactgca gagatgacaa acgaattcct aaagaaagta aaaaatgaaa ttccagcaaa
     5161 ccccgcccat ccttttgcgt aattttccca ttgaaaagtc tctggaatca aattcagcga
     5221 accactcata atctcttgat ccgttttaaa agaacctgcg atcatccata agaccggata
     5281 aatcatgact agacctaata gcaaaataaa accgtattga aaccatttcg ttactgtatt
     5341 attcatttct tcctcctctc attcacacaa cgacccattt ttttgatgat ttgaaaatca
     5401 gaaaggtgac agctcccata atcaacagca tgatccacga aatagcacta gcatacccca
     5461 tagaattaaa agaaaaagct tggttataca cataaagagc ataaaagttc gtggcatcat
     5521 tcggaccacc ttgggtaatg ataaatgcct gcgtaaaagt cataaacgcc gcgattgtct
     5581 gcataaccaa gttatacaaa atgatcggcg aaagaagagg taacgtgatc ttgtaaaacg
     5641 tctgccattt gttcgctcca tcgatctctg ccgcttcata atacgagcct gggatctctt
     5701 tgatacctgc agcaaagatg atcattgccg aaccgaattg ccaagcagac atcaaaacaa
     5761 gagggatcat gaccagtttt tgatcgctaa accaggaaag cgcaggtgcg cctaatcgtt
     5821 ccaggaaaat attcactaac ccgtttttag aaaacaactc ccgccaaact agagcgatag
     5881 aaatactgcc accgatcaaa ctaggcacat aatacaaaga acgaaagaaa gtcgttcctt
     5941 tgatattcct cgttaaaaga taagcaatca atagaccaaa gatcagtttc acaggtacag
     6001 atgcaaatac atagagaaaa gtgactcgga tggagttcaa aaaacgggca tcactcagca
     6061 tctttatata attatccaag ccgatccatg agactggatt tcccaaacga taatcggtga
     6121 acgaataata aagtgacaag atcatcggta tcactgttat caatagaaat cctaggatga
     6181 atggagaagc aaatacgtaa ccgaccacat tgtcttgatc caagaaccgt tttagttttt
     6241 cttttttcat gccttccttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     6301 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     6361 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     6421 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     6481 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     6541 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     6601 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     6661 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
     6721 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
     6781 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
     6841 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
     6901 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
     6961 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
     7021 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
     7081 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
     7141 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
     7201 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
     7261 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
     7321 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
     7381 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
     7441 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
     7501 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
     7561 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
     7621 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
     7681 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
     7741 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
     7801 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
     7861 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
     7921 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
     7981 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
     8041 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
     8101 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
     8161 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
     8221 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
     8281 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
     8341 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
     8401 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
     8461 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
     8521 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
     8581 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
     8641 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
     8701 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
     8761 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
     8821 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
     8881 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
     8941 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
     9001 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
     9061 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
     9121 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
     9181 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
     9241 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
     9301 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
     9361 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
     9421 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
     9481 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
     9541 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
     9601 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
     9661 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
     9721 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
     9781 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
     9841 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
     9901 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
     9961 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    10021 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    10081 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    10141 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    10201 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    10261 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    10321 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    10381 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    10441 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    10501 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    10561 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    10621 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    10681 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    10741 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    10801 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    10861 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    10921 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
    10981 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
    11041 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
    11101 atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
    11161 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
    11221 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
    11281 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
    11341 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
    11401 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
    11461 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
    11521 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gctctgttgg
    11581 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
    11641 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc actatcttgc
    11701 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
    11761 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
    11821 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
    11881 aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac ttgcccaatc
    11941 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
    12001 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
    12061 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agaggaaagc
    12121 ttttgaacac cgtttaagaa agaggttgtg agcctaccgt tcagctccga gtcacaagga
    12181 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
    12241 gacaggagaa caccgtttaa agaagaggtt gtgaaatcag ctttttgacc tgaatattag
    12301 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
    12361 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    12421 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgttaaaat
    12481 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    12541 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    12601 cacattggcc tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg gctgtctgag
    12661 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    12721 atgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    12781 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    12841 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    12901 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    12961 aaccgaattt actactatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    13021 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    13081 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    13141 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    13201 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    13261 tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa tgaaaaacta
    13321 gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa tctctatgca
    13381 atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg tttttttatg
    13441 tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat tatctgctgt
    13501 tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta ggatcatcgg
    13561 caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct attttcacaa
    13621 tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga tcgtcgtgtc
    13681 agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt tgctgattgc
    13741 gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac tagatacttt
    13801 tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa
    13861 gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat tgctaagaac
    13921 aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg aggactaatt
    13981 ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt cctccatttt
    14041 tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt tctgcctctt
    14101 ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa ttctctacga
    14161 tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat
    14221 ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg cttcctgtat
    14281 cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc atcaattgcg
    14341 gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg aaggcattgg
    14401 cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct tcggcttctg
    14461 cttgtccagc taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc acacggaatc
    14521 gttctgcttc ggcttcagtc acttctttga ctttttgagc ttgtgcttct tgttctttag
    14581 cgtaccgatc ggcatctgct tttttcttca cttccgaatc atattgcttt tcacgacgtg
    14641 tgatttcttt ttcttccagt tcgatttgtt tttggcgttc tacgatttta atctgcattt
    14701 cctgttcgat tacttgttgt tgtgctcggg cactttctaa attgtaggct tggtctgctt
    14761 ttgctttagc aacatcttgt tcttgtttgt aggttgctaa tttcagttct ttttctttta
    14821 atgattctgc gatctctgtt tgtcgctgta attcagctgc ttgtgattct ttttctgctt
    14881 gagctttttt gatacgcgtt tcttttagag cttccgcttc tgctatatct gcatctcttt
    14941 tgacttgggc gattcttggt ttccctaaag agtcaagata accgtttttg tctctgacct
    15001 ctttgatagt gaaagaaacg atgatcaacc ccatttttgc aaggtctaca gaagcgactt
    15061 cttgtacgct ttgactgaac ttatcgcgat tttgatagat ttcttctaca gtcatagaac
    15121 caagaatcga gcgcaagtgt ccttctaata cttctcttgc ttcattttcc agttcttctc
    15181 ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc ttctacagat gaaccgattt
    15241 tgatgatcga agtaccatca cacatcaccg gtacgccttg ttcggtatac acttcagggg
    15301 tggagacgtc caatttactc gataatagac tgatccgatt cgaacgttga aaaactggca
    15361 agacgaatgc gccacctcca cgcacaattt tgattttgtt attgctttca tcaatgtgca
    15421 catttttgct tcctaaataa cttccactga tgatcaacgc ttcatccggt tttgctgtct
    15481 ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat caaagctgcg attgcgatcg
    15541 cgataaaaac aactggtatt tccatttaac acacgctcct ttaagctttt atcatgcttt
    15601 cataaggcat gacataacag attctttcct tgatctcaat gatcaatact ttcgttcccc
    15661 gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg acgactaatg gaaccggtaa
    15721 cacttttcaa ctgaatttct cccatacctc gtacagggat cggtgtgatc acaacagcta
    15781 cttgtccttc cagagttttt tcagaagacg acatcgttga ttcggcatgt ttcatcggca
    15841 tcaatatata gaaattaagc aaaaatacca acactatcga tatgcctcca ctagcaataa
    15901 ataaaaataa agatgattga tctgttaaac gctcacctag aaaaccaaat aagcttgtga
    15961 aagttaacca agggataata agcatcggat caagcgggcc atcaaaatcg aatacatctc
    16021 caaaaataat caataggact gccaatcctg cacaaatcag caatgtatac caataaatcg
    16081 tctcaagtgc gattccccct atcatataca cgcctccttc catttctacg tctatcatac
    16141 caatgtttga aaattctgta tagcttcttt taccgactca gtggtgtttt ttataggaag
    16201 gttgtgttta tcgttgacaa tttgttaaat ttttgttttc atgttaaaat tttcacaata
    16261 aattctttcg aggtagccta tgtttccttt ttttaagaaa aaaccattaa agctccagaa
    16321 cacgatcatc cttgtcgttt gtttctccac attcgtttct ttgattgttt ctgccatatt
    16381 gatcaggaat tttattcttt cgatgaaccg atctagtgtg tcagataaaa tctctagcat
    16441 tgctaaaatc gttgccaatg ataaactcat ccaaaaaaag ctgcagactg gtgacccgac
    16501 acatgcgatt caaggaataa caaatgaaat catgattgca tcgaatttag attttatcgt
    16561 tgtcatggac cctgaaggca ttcgatattc tcacgcagac ccaagttaca tcggtcttcc
    16621 tttctcaagt catgaagatg tgaaaaaagt gattggagga caagaatttt tctctgaaaa
    16681 agaaggaatg ttagggttcg gcttccgcta tttcgtccct atttattatg atcaagagat
    16741 tattggtgca gtttgtgttg gcttaacaaa caacacgatt gacaatcaag tcaaagatgg
    16801 acaaaaaatg attgtttttt cattgattat tggacttagc gtaggcatca taggcgccgt
    16861 ttttcttgcc caaaaaatga aaaaagtact gcttggactt gaaccacaag aaatagcaga
    16921 acagttaagt gaaaaaacga ttatcgaaaa tgaagtagat gaaggaatct tagccatctc
    16981 ttctgataaa actatcctct tgatcaatac acctgcaaaa aacattttct accaaacaga
    17041 gcagctattg gatttcgaag taggaaaaaa atcagtgata atctatatga cgttttgttt
    17101 aaagagacga ttgagcatca aacgaccgtt cggactcaat ccatgtattt aaatgggata
    17161 gaaactattt ctagcgttac acctcttttt agcaatgacc gttttttagg agcagtcgca
    17221 actttacgaa atcgatcaga agtaatgaaa ttaatggagg agcttggggg gacgaaacaa
    17281 tatataaact ccatgagagc tcaaacccat gaattcatga ataaacttca tgtcatttct
    17341 ggtttgattg atttgaagaa gtatgaagaa gtcacccatt ttatccagca gctaaatcaa
    17401 aattataaaa aagagattgg ccaagtctca agtctgatca aagtacctgc aattgccggt
    17461 tttttattag gcaaaataaa cgaagccaaa gaacaatctg ttgaaatgct gttagaaagc
    17521 gattctttta tccctaaaat ggagatggat gactccatcc attcactttt gcaagtgtta
    17581 gggaacttac ttgataatgc aaaagaagcc gtgctacaaa aacatgaaag aaacggacag
    17641 gtccggattc atttaaacta tgacacagaa gctgaagtat tcatcgtgac cgtctgcgat
    17701 ggcggtgtcg ggatatcaga agaaatctca gaaaaaatat ttacgctagg tttttcgacg
    17761 aagggaatca atcgtgggtt tggattgaat ctcatccatt caatcgtcaa acgccacggt
    17821 ggatttttag aattttccaa acaaacaaat ggcggtacaa tcgtctatct agaattacca
    17881 aaaaaggaag atcaaaatga tgacaaatgt cctgataatc gaagatgatc caatggttgc
    17941 agaattgaat cgtacttttg tggaaagcat ccctaatatg aacgtaatcg aaagtttcag
    18001 aaatgtagaa gaaggaaaaa aatatttcag acaaaaaaac gcagatctgc tgctgcttga
    18061 tgtatacatg cctggcgaaa gtggtatcca ttttttatct tggcttagac agcaaggaaa
    18121 tgatatccca gtgattttga tcactgctgc agatgatatc gaaaccataa agaatgtgat
    18181 ccgctacggt gccttagatt atttgatcaa accgttcact ttgcaacgtt ttcagctggc
    18241 gatcgaaaag tatcttgctt tctctcagac aatccaaacg actgaaaaag ccgatcagaa
    18301 attgatcgat caagtattca accacaccac tcccattcac gaaataccat cattcatcga
    18361 tttgcctaaa ggtctgtcaa aactgacttt aaaaaaaata agtgcattta ttctaaaaga
    18421 agatactatt ttttcaacaa aagaactggc tcaaaaaagc tcactgtcac gtatatccgt
    18481 caagaaatat ttgaattttt tagtagaaca agatatattg aacgaaagaa tcaaatattt
    18541 ggaaatcggc cagccgctaa cctgttatga attgacaaag aatgggcgga aaaagctcac
    18601 agattactgt tgagtcatcg aattatccca tacaaaatag tccaagtata cagaagtaac
    18661 aactgtttac ttggactatt tttaaacatt ttattgacga atatccatgt tttcttatct
    18721 taattgacgg gctttaggaa gagatacctc atttttcgtt tttccagttt ctattatttc
    18781 acgaaagttc tcgaaacttg tcgaaatcat atttgataat gcctcatctg tatttgaacc
    18841 tacatgcggg gttacaagaa ctctagggta catttctact aattgctgga tagcagggtc
    18901 tggaatcgtc tcccatggct tgaaggatcg gaaaaaaacc tctgtttcat ttgcaaaaac
    18961 atcggttgca aaaccagcta aatgattgct tttcaaagct cttaaaatag cttcattatc
    19021 ttgaagctct ccacgggctg tattaatcaa gatgctacct tttttcattt gagctaaaaa
    19081 tccttcattg atcatttgat cgttttcacc tgggatatga ggtacatgaa tactgacaat
    19141 atcgcttttg gctaataact cttcaagcga gtggaacgtc aatacttttt tagcagcttc
    19201 tgtctgatga atatcgtagc cgatcacttt ggctcctagt cccttaaaca aaacagcttc
    19261 cgtcaaccct atcctgcctg ttcctatgat ccctactgtg caatttctga tttcacgact
    19321 aaacatttgc tcatccactc gaaaatcttt gaaagatgtt cggatcgtcg tatacgcagt
    19381 atttctcaaa agcatcatgg caaaagtcaa agataactct gctatcgcat ttggcgaata
    19441 cgatggtaca cgggctactg tcataccaaa atcagctgcc gcttgtagat cgatatgatt
    19501 gactcccaca gtccgtgtaa aaacataacg aatcccatat tcctgcattt ttgcaagatt
    19561 ggtctcatct gctatacaat ttcccctcaa caatacagca tcatggtcct ttgctgacac
    19621 gatattgtca tgtgtcaaca gttcttcata aagactgaga tcataatggt aaatattcaa
    19681 tttttcaaaa aaatctatct cgttcggacg aacaccgtaa catgcgattc tcatactttc
    19741 ttcctccatt tcttatgaaa ataaacctat gccatgaatc atttgtaata caacgatcca
    19801 tacaggtgtc agtaccacag caaaaatagt tgacagtaag gatgcattgg aggctaaaag
    19861 tgcctcccta tcaaaactga tagcataggc tgctgccact gttgcagctg gtgtagccat
    19921 catgataacg attgttgcca gtgcttcaaa attgactggt agaatattcg ttgccgtcaa
    19981 aactgctaga atcacgatat tcaatgcagg tacaaatacc actttgacta gactgtaata
    20041 ccatgaagtt ttttcagatg cagctttttt gaagcttact tcacctaatg ttgcgccgat
    20101 tgctagccac gccaatggtg aacttaaacc agctaaataa tccatcggct gaaatagcca
    20161 cggtgcagtc tggtcaattc gcaaaaacgc tacttggctt atctctcctt ctgcacctgt
    20221 aactgacact tgtggcagat acccttggaa cagccaaatg aaaaggccgg caaaagtagc
    20281 gataatgatt ggattgacga acatcgcttt gatattttta gcttccatct tcaagccact
    20341 catttttatg tatccataag aatacaaaaa gattcggtag ccaatattga aaattgagga
    20401 atacatcacg ccgacaggac cataaatcgc actgacgata ggcgttccga aaaaggtcgt
    20461 agaaccaaag atcgtcagga cacgcaacgt tgtttgctta tcccctttat agcttaagaa
    20521 aaaaggtttt gaaactagaa taagaataat ataaataact aatccccaaa tcaagacatt
    20581 gattccttgc atcagtaatt ctgtattgat atcttgcatg aaagctcgaa atgccagtgc
    20641 tggcaaagcc actgttaaga cgactttcga tagaattttt ccaacagatt cagaaaaaat
    20701 cccctttttt ctacaaacaa accctaataa tataataaaa actgttgaag tgattgcact
    20761 gacaatcgac atatctgtca aagtcgatcg aaaaatgtca aataagttca tctttctcct
    20821 cctaattatg ttcatttatt cacaataaaa aaagctttta ttttgctaat acgtattaac
    20881 atttttaatt ttatctttac attgttactt attgaacaat aggaaattaa agaaactttt
    20941 gaaacttatg taaagtgatt tttactagaa cacaaaaaaa ccttaaaacc aaacttaaga
    21001 agtttagttt taaggttaac ttaaattata ttatgcgtgg aaacgttcca cgccatctag
    21061 ataagttgct tccaatgtca tatcagggtt caatacgata aagtctgcat cgcgtccttg
    21121 agcgattttt ccacatgtat catcgatttt acagctgatt gctggaatca gactagccat
    21181 catgactgct tgttctggtg ttgctaatcc ccagtctaca acatttttaa ttgcttcttt
    21241 caatttcaat atacttccag ctaagttgcc agattctaga cgtgctgttc cttcttttac
    21301 tactactgga aattcgccta aattgtaatt tccatcaggc atcccaccgg ccatcataca
    21361 gtctgtaatt aatgcgacat gatcatgtcc tgctttttcc attaggattc ccgcagcatt
    21421 tggatgtaca tgatggccat cacaaatcaa ttcagaaaag acatgttcta aagataataa
    21481 cgcacccacc atacctggtt cgcgatgatt caatccacgc ataccattga aagcatggac
    21541 aaatacactt gcgcctgctt caacagcttt ttgcgcttct tctagtgtag cattactgtg
    21601 tcctagagaa acaactactc cttcttcagt cacttgtttg acaaactctt ctacaccttg
    21661 acgttccggt gctaaagcaa tttttttgat taaaccacct gaagcttctt gccattcatg
    21721 gaaagtatcc aaatcaggat ccccgaaata acttgggttt tgtgcccctt tgtgttcttc
    21781 tgtgaagaaa ggaccttcaa aatagattcc tttgatttta gctcctggtg cttcctgata
    21841 cattttgccg attgtctcag ctacatcttt taaacgttct ttgcttgacg tcaatgttgt
    21901 tggtaaaaat gaagtcactc cacaagataa cagaccttca gacatcactt tgatgccttc
    21961 tgcatcatta tccattacat catgattcat atagccgtga atatgtgtat ctaccaaacc
    22021 aggagcaatc catttgccgc tttggtcaat gatgttgact tctccctcag gtagttcttt
    22081 ggtgtattca ccaaaaagtc catcggtaat ttcaagatag cctgcatgtt tgacacctga
    22141 cgctaaaaag aatttgtcag catagataaa agttctcatt ctattctctc cctttgtttt
    22201 tctacctcta aagtactctt atttagagaa gaagtcaaga atggtctaca ccatttacaa
    22261 attttttgaa atttgtttca cagcttgtgc taagccttca tagaatgctg ctctttctgg
    22321 ttcttcttta cgcttgtata ttaagctttt aagcacaaat aaatcattga ggtaatagta
    22381 gctattcttc tcttgagccc aatagattcc ttgattcacc tgttctaacg cttcgtcgat
    22441 ttcattttga tggaataaga aatttccata attataaaaa atttgcgaaa attcactttt
    22501 tgatcgttca tcagctgatt gatgcagcag ttggatgctt cgactcaaat agtagcgtgc
    22561 ttcagccgtt tttcctgcca caccatatat ttttccgata caactaatca gttgtatttc
    22621 tgtgtcagaa acatggattc tttctttttg gctagagtag cttaatgctt ctttcaaata
    22681 ataaagggac tgagagatat tttgacgcaa cgtgaattcg caaacaccga gataataata
    22741 atagcgttga aagtcagttg cttgatacag attttcagaa atatcagaag attttagtac
    22801 ttgttctaac ttccgataat cacgtttttg gaaacaacta tccaacagtt caaataattt
    22861 atcgtttgtc cgagtagttt ttcgagagag attcatcaca tgatcaatcg ttacccctag
    22921 ccgttgacaa atctgcatca tcaccaatac attcggcagt tcctcattat tttcgatccg
    22981 acttaataca ctttgtgaac aaatatcatg cgaaagcatt ttttgcgtca ttttttgttc
    23041 ttttcgaatc tctttaatga tcgttccaaa ggactcgatt aacgtttcca taagtatcct
    23101 ccccaaaata tgcgtattcg cattctattc gtattttact ttaggaagtc gcaaacttca
    23161 agaaaagact tgcaactaaa taatatactt tagttattta agaaaactcc actagaaaga
    23221 gagcctcagt gtgtttgtct gtggccctcc tactgaaaaa tttggttttt aaaatcattt
    23281 ttggcgatag gttgctagga attctctcat tttcttagct gtttgtttca aatcatccat
    23341 tttaggaaga taaacgattc gaaaatgatc tggctggttc caattaaatc caccaccatg
    23401 tacaagtaga atatgatgtt catgtaaaaa gtccaaaacg aatttttcat catccaaaat
    23461 attgaatcgc tttgtatcaa tttttgggaa tatataaaaa gcagcttttg gttttacggc
    23521 tgaaagacca ggaatatcat tgattgcatt atagatatat tctctttgtt catagatcct
    23581 tccgcctggc aggagcagct catcgacact ttgataaccg cctagcgcgg tttggataat
    23641 ttgctgagac aatacattgg aacacaaacg catggaggac aacatattca gcccttctat
    23701 atagcctttg acgtgtgact tatctccact taggaccatc cagcctactc gaaatcctgc
    23761 tacacgatga gatttggata gaccgttcag cgtaacaacg aacaaatcag gcgctaatgt
    23821 agcaatcgga atatgttcta gaccatccat cactaagcga tcatagattt cgtcggaata
    23881 aataatcagg ttattttgtc gagctacttc aacgatttct tctaataatt cttttggata
    23941 aagtgcccca gtaggattat taggattaat gataacaata gcttttgtcc gactcgttat
    24001 ttttgatttg atatcatcaa tatcaggata ccattctgcc tcttcatcgc aaatataatg
    24061 aacaggtgtg cctccagcta atgcaataga agccgtccac aacggatagt ccggcattgg
    24121 aaccagtact tcatctccat tgtccagcaa cccctgcata cacatcgtga tcaattcgct
    24181 cactccgttg ccggtgtaga tatcattgat cgtcacattc ggaaaatgtt tcagttggca
    24241 atattgctca attgcttttc tagcagaaaa gatccccttg gaatctgagt agccttctga
    24301 gtcacgggca ttcatgatca aatcacgaat gacttcgttt ggtgcttcaa atccaaaagg
    24361 tgcgggattt cccgtgttca attttaaaat actaatgccc tcttcttgca tccgctccgc
    24421 ttcttctaga acgggaccgc gaacatcgta actgacacct tctaatttgt tggatttttc
    24481 aaattctctc atcacttagc caatccctca tttcaaaaag ttagaacttt ccaaattttt
    24541 tgttatttac tatttcaata tcatactcca tcctagaaaa ttttgccact tatttaggaa
    24601 aaacgtaagt attaaaaaaa cacaaacaag cgttgagaac ttaattgtca tgatagtcat
    24661 tccatagatt cgctgcccaa tccattgcaa ttccacaaat gattcctagc ataaaaccaa
    24721 gcaaaaaacg tccgaatgca agtccaatca atacacctaa gatcattccc agtcctgaaa
    24781 attctccgtc atatttctcc atcttaataa gtcctcccat ttttgagctt gacaactatg
    24841 taaaacctcg ctatcattag tgtgttatta ttacgcagat gtgttggaat tggcagacaa
    24901 gcatgattga gggtcatgtg agcgttagct cgtgtgggtt cgagtcccat catctgcact
    24961 acaactcgtg aagttaattt tgcttcacga gtttttttgt ttgaacgttt ctttatgcca
    25021 ttatatacat atcactgtat cctttacaaa aagaacgtac aatcatgtaa acagcctgag
    25081 gtgagtatat gaaggtcgga aacaagctac tcgtagaaac ttgcaagtgt taaaacaatt
    25141 aatcgaaaaa cgagtaaaat ccctcaccta atactaaagc gagggatttt ttgtatatta
    25201 tctgtttatc accgttatta taaaggtaca aataccaagc acggttaatc caaattgcat
    25261 aaattcattt ttttgtacac tcatgctttt cattagctct ttaactgcca ttagtactaa
    25321 gatggcgata acaacataaa gtatatagta cgtccgcatt ccggaagccc atactgtggg
    25381 agaaaatccc ataattaacc ttgaacaaaa tcccattatc atcaaaataa ctaaaaaaat
    25441 agaagttttc ttattgtcaa aacttaaata aattccaaca attaaacata gtaataatgc
    25501 caagactact aaagtagcta tccaagtacc tggataaaaa agtgacagtt tcgttcctaa
    25561 ctctgtaaag agattattca ggttactact actccaaatc attgcccttt tatttccatt
    25621 gacgtaagta aagctttgac ccattgtatt accaaaataa ataatcaggt ttaagaaaaa
    25681 cggtaatgca gtcaatatac gcacataata gttttgacat ttcctatatg ttagaaggaa
    25741 aattaagaaa aatagcaata aaaataagat attcatgtct aaaaaaaacg gctttccaaa
    25801 tgatgaaata cctaaatcta acttattaag aaagttgaaa tttttatatt caggaaacca
    25861 tttattaatt tcatgtgcag cacgtaatgc attccctgga gttgtcaaag agaaaattaa
    25921 ttctgctaag cttattattg aaaatactga taacttataa ttatatcgtc cattgacaat
    25981 caaatataaa ctaactatac ttgttagaac aaaaaaacag gcgttgactt gttcttgatt
    26041 agttaaaaat atcaataaag gaatacttac ccaatatatt tttctattta tttcttctcc
    26101 tcttaagagt tgaaaaaacg gataaaaagt tagtaaacta aatgctactg gccactgata
    26161 gttcaaggtg gttgctctcc aaccggtttc ccccattact gtcaatggta ttaaacagta
    26221 tatagagcaa accaataata aatttttaac atttattttc tcaaaaacat acttgcaaag
    26281 taatactaca attcctaaca taatcgtaga atttaataat ttccatagca taaagtgttt
    26341 agaaaagaac attaagaata gttctataat aattcttgaa ctccatgtct tataatgttc
    26401 tatcgaagca cccaaatagc caagttcatc taatctactt gcataaacta cgtcatccgc
    26461 tctcatagtc ggcatataca tattaaagag taaaaagata ccccaaatcc ctattagtaa
    26521 caagatcagt tctttgttat tcgttatttt tctattatgt ttgttcattt ttatactcct
    26581 tcttatattt cctaatagag tataacatag ctttaagcat tacttaaaga taacttaaaa
    26641 gttatctttt aaatacttaa ttgttaaata ccttatttga tgcaaaagaa atctacgata
    26701 gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg acctatggat
    26761 tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata ttgcttttaa
    26821 aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag gtttaactgt
    26881 cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt acgactatga
    26941 taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat cctacaattc
    27001 cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc cccattgagc
    27061 gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc ctcaaatgag
    27121 tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata tatagattga
    27181 ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc catctttcca
    27241 aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat tctttcatct
    27301 tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat tttgggtttc
    27361 cctaacacat gataggatct cacaacaaat tgctttctat tattataata acctgtaacg
    27421 actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga agcaatcaaa
    27481 caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg caaagtacgg
    27541 atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc attatacgaa
    27601 catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt tgtctattac
    27661 ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact aaaaaaaaga
    27721 ggcagcagta tagattactg ttgcttcttt actaatttag gtttacttgt taagaaagat
    27781 tatttacgca aatgttcttc aacaatttgc tgagatttat cagctgtatc ttccacttca
    27841 tcttttgctt tttctaactt ttgaatatat tgtttcaagc ttgagttttc agcagttgct
    27901 tcgtctaatt tcttttgtgt atctgttaac tgagtttcta catttgtttt ttcttgtgtc
    27961 agtgttgcga tttgttgatt caaaccattt acagtatttt gtaattctga aagttttgct
    28021 tgtgcttctt gtagtcttgc tgtccaatct gcattcagat gattgatttc atcatttttt
    28081 tggttgatct catctagttt gacattgatt tcattttgtt tgctttgtag ttgtgcatcc
    28141 ttgctgacta attgactttc taagttttgt ttttctgctg ccgattgatt tttttggttt
    28201 tctaattcat ttacttttgt ttggtattct tggacatctg cctttgaatc ttgcaatgtc
    28261 tgttcttttt gtgctagtcg agcggtaaga tctgtaagcg cttgctcttt atctgtcaat
    28321 gtatgatcaa gctgtgtcag attctcattg attgtatcaa tatctgtctc tccaccccac
    28381 aaaataattt catttgcgta ccgcgtgcct gtgtagccaa ttagacatag aaatgcaacg
    28441 acaatcccac cagtaataaa tttctttttc atttgcatag ctccttaatc caatactttt
    28501 acatgctaaa agtaagtgat ctcaaacaca atgtcaagcg tttttaaatt acatgtgtaa
    28561 ttttaatata aatactatca aatactcatt taatcctcct tttttatcta tttaaaaaat
    28621 cttttttata gagaaaaatg caggttaata ctttcttaaa taaatattta tggtgaacaa
    28681 aa
//
LOCUS       DANKAZ010000030        27973 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000030 DANKAZ010000000
VERSION     DANKAZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(399..1343)
                     /locus_tag="PWY67_001226"
     CDS             complement(399..1343)
                     /locus_tag="PWY67_001226"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2057522.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1639..2583)
                     /locus_tag="PWY67_001227"
     CDS             complement(1639..2583)
                     /locus_tag="PWY67_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2057523.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2804..4018
                     /locus_tag="PWY67_001228"
     CDS             2804..4018
                     /locus_tag="PWY67_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2057524.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4040..6055
                     /locus_tag="PWY67_001229"
     CDS             4040..6055
                     /locus_tag="PWY67_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL2057525.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6138..7511)
                     /locus_tag="PWY67_001230"
     CDS             complement(6138..7511)
                     /locus_tag="PWY67_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2057526.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7537..7821)
                     /locus_tag="PWY67_001231"
     CDS             complement(7537..7821)
                     /locus_tag="PWY67_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2057527.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7823..9838)
                     /locus_tag="PWY67_001232"
     CDS             complement(7823..9838)
                     /locus_tag="PWY67_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2057528.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10017..10322)
                     /locus_tag="PWY67_001233"
     CDS             complement(10017..10322)
                     /locus_tag="PWY67_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL2057529.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10411..11442)
                     /locus_tag="PWY67_001234"
     CDS             complement(10411..11442)
                     /locus_tag="PWY67_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2057530.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11649..12524
                     /locus_tag="PWY67_001235"
     CDS             11649..12524
                     /locus_tag="PWY67_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2057531.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12676..13518)
                     /locus_tag="PWY67_001236"
     CDS             complement(12676..13518)
                     /locus_tag="PWY67_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2057532.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13610..15487)
                     /locus_tag="PWY67_001237"
     CDS             complement(13610..15487)
                     /locus_tag="PWY67_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2057533.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15484..16905)
                     /locus_tag="PWY67_001238"
     CDS             complement(15484..16905)
                     /locus_tag="PWY67_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2057534.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16908..20297)
                     /locus_tag="PWY67_001239"
     CDS             complement(16908..20297)
                     /locus_tag="PWY67_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2057535.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20554..21945
                     /locus_tag="PWY67_001240"
     CDS             20554..21945
                     /locus_tag="PWY67_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2057536.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22018..22665)
                     /locus_tag="PWY67_001241"
     CDS             complement(22018..22665)
                     /locus_tag="PWY67_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2057537.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22673..23359)
                     /locus_tag="PWY67_001242"
     CDS             complement(22673..23359)
                     /locus_tag="PWY67_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2057538.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23365..24438)
                     /locus_tag="PWY67_001243"
     CDS             complement(23365..24438)
                     /locus_tag="PWY67_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057539.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24660..25334
                     /locus_tag="PWY67_001244"
     CDS             24660..25334
                     /locus_tag="PWY67_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2057540.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25339..26679
                     /locus_tag="PWY67_001245"
     CDS             25339..26679
                     /locus_tag="PWY67_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2057541.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26828..27814)
                     /locus_tag="PWY67_001246"
     CDS             complement(26828..27814)
                     /locus_tag="PWY67_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL2057542.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8166 a         5270 c         5064 g         9473 t
ORIGIN      
        1 gttttttatc aaaaatcgtc atttttccat caaaatctgc ttatgcaaaa ttataaaaaa
       61 attttgcagt ttttcttact ttttttgtcg tttaattgaa tgataaaaac cagtttcttt
      121 attcagttaa tcaaatatga acgaattaga tatgaatcat tgtatttgcc gttgcggaag
      181 gattttttaa ttaaccacag ataaacattg ataacgattc gcttattgta atcaacagat
      241 taaatttcat atgttcatat gtacggatac attttcggac cgattaattt taaccattct
      301 gtttatttgc ttacctatta gatatcaata aaaaactgct taacagctat ttcagctgtt
      361 aagcagtttt aggattttta gttcatattc attgtttctt ataatacagt gttcaatact
      421 tctcttaaag cacttgttga tttgctgaac attgcttttt cttcttcgtt gattgataat
      481 tcaacaactt cacgaacacc attttcgtca acgattgatg gcacacctgt aaagatatct
      541 ttttcgtcgt attcaccagt taaataagca gatacaggca atacagcttg ttcgttattc
      601 aagatagctt ttacgatacg tgcagtactc ataccaatac catagtaagt tgctttttta
      661 cgatcaatga tctcataagc agcattacgg actttatcag caatcacatc taattcatct
      721 tgggcaatac gatggtcttt ttctacaatt tcaaacactg gtttaccacc gacagttgta
      781 tgtgaccatg cagcaacttc tgaatcacca tgttcaccta agatatagcc gtggacgctg
      841 cgtgggtcaa cttttaattt caatgcgatt tctttacgga aacgagttgt atccaatgtt
      901 gttccagtac cgataacacg tgatgttggc agaccagatt cgttccaagc gatatatgtc
      961 aaaatatcta ctgggtttga agcaacaacg ataataccgt cgaatcctga tcccatgatt
     1021 tcttttacga tttggcgcat gattgacgca tttgttttaa ctaaatctaa acgagattga
     1081 ccaggttttt ggttgatacc agctgtgata acaacgatat cagcatcttt acattcttca
     1141 tagccacctg accatacagc cacattttca tcgccccaag ccataccatc taaaaggtcc
     1201 aatgcttccc cttctgcttt ttcttgattc acatcgatca atactaattc attagagatt
     1261 ccttggttga tcattgcata agcgatactt gttccaacaa aacctgtacc gacgattact
     1321 actttacgac ttgttttttt catgaataaa cagctccttt tcataactat ttgttacttt
     1381 catgaaaagt atagtcttgt ttattcattt tatcaacatg ttaattataa tagttgttat
     1441 tataattttt gatggaaacg aattcctgaa tacgcataac agataaatga caaatgttta
     1501 cacctatatc ccctgataat acaaccaaaa gtttagaact gaaaataaat ccaaaaaaat
     1561 tcgctgcaac gatataataa gacagcaatc gcacctaaaa caattttcct tcatatgctt
     1621 tagtgaaaga ttttcttctt aatgtttatt tctccattca ttgggagtac atgttgaata
     1681 cttcttgaat aatttgaata aagaagtcgt ttgtttatat ccgatacgat tagcgatttc
     1741 ttccaacgaa aaactcgttt cttgaatcag ttccaacgca aagtcgaatt ttttctttag
     1801 aaccagttct tgaaaggaag catcaaaata cattttcaat ttattactaa gatagaccgg
     1861 attgtagcca aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat gcttatcgat
     1921 ataatctaat actctaagca tctcattttc tgcgcatcca tctgctgatg aaaagctttg
     1981 ttgttctaat aaatgagtca attcgatcag taatagtgat gtatagagat ttaacgaacg
     2041 gatcttatga ctgtatgtat caaaataaat caacagcata ttttcaatca aatgcgtcaa
     2101 acgacttgtt tttccgccgc gaaaatgaat gaattgtgta tgttcccctt tttcttttga
     2161 tgcatttatc aaaaaagaag aaagtagatt atttgtgctt gccaaatgat tcaatatctc
     2221 tgtagataag gtttcttctt ttaataagat gttgattaaa atatcctctt gccctgtgta
     2281 ttcgattctt tgagtagctt ctttatcaat gatcaccacg tcactttctt ccatctctat
     2341 cctttctcca ttaataattt gtaaacatgt accgctatac atataattga tttcaacaaa
     2401 atcatgtgta tgctcgggaa cttccgcata tcgacggtgt ttactgatat gaatatttcc
     2461 ctgttgaaat agatcccctc ggtggatttt tggcggtgtc tgaagatggt aagctaagtt
     2521 taaatcattg atattcattc cttgttcacg ctgcagagtt tcgattgatt cttcgttacg
     2581 caaaaaaaat gtctaattca gctagttcca attgactccc tcctttttgt aagcggtttc
     2641 tttcgcttat tagttcacgc tgtactgcta ttatatgcaa agctattttt ggtttcataa
     2701 agactttcta tggttgggac atgaacttcc tgtgattccg ctattgagag aataaccact
     2761 tcatactata tttttgataa gcgcttacaa aaatgaaagg aacatgctta tgaataaaaa
     2821 gatgatgaaa ttttctctgc tgtcagtctc tcttattttg acttctgctg gtgcagtcac
     2881 gtctgcaatt cccagtatgt cagctactta tgccacgcgt tctttatctg ctgttgaatt
     2941 attaacgact gctccctctt ttatggtcac tatttttgtt ttgattagta gttttattgc
     3001 taggaaaatc ggacaaaaac aaactgtgat catcggatta gtgattgcat cgatttgtgg
     3061 tatcctcccc ctcttctcac aaagttacag tattgtctta gcttcaagag ctggactagg
     3121 tattggcttt ggattgatca attctttggc agtcagcatg attgctgcct tttatcacgg
     3181 agatgaacga gcctctatga ttggttttca aagtgcattt caaggatttg gtgcagcttt
     3241 aatgacattt gttgcaggac agctagtaca gttcggttgg caatatacct ttttagtcta
     3301 cgccatcagc ttacctattc ttgtcttgtt tgttttgttt gttcctacac ctgaaacaac
     3361 tgaacttcaa tcgcatggca cagccccaac aagacaagta atcaaaacga atcctaaaat
     3421 gattagttat ggtatttttc tcttgctggt catcgtggta tataatgctg tatcgattaa
     3481 attagccaca gtcctgacga cttattctct aggaaacgaa actaatgcag cgaatatctt
     3541 aacaatcatg caattggcaa gtatggctgc cggagtttgt tttggctggc ttcagacaaa
     3601 agcacatcgc tatatgctta cactttcttt actactgatg ggaatcggct ttgtattgat
     3661 tgccactgtg ataaacctgt atgtgattgg gtttggtgcg atcctaacag gaatttcttt
     3721 ttcgttattc attccttact tattcaacca agtaagtatc caagcgccca agtacgcgga
     3781 gagtttcaat acttcaatct tattggttgg cgctaattta ggtactttca ttgcaccata
     3841 tggtttgctc tttctctctt ttgcttctct tgggagtaaa acacttgctg ttttcgtcaa
     3901 tggcagtatc ttgctgttga ttagcgcgct tattagcctt ctagtagtac acaggacgaa
     3961 tagaaagaca gtgataagag tagatgaaga aataaaagaa caattgacaa caaaataaat
     4021 ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc tttggtatcc
     4081 tggagacttt gagatccgcc atggactgct acaaaatttc caacgggaag aacgaggttt
     4141 tgactggcca gcgtactggt acatggatga ttgccatcgg aacgtcaagt ttaagagata
     4201 ttactttctt gatcaacctt ccatgtttaa agtgacgatc caaggagttg ggtatgtaga
     4261 aattaatgga caaaaacatc catgtggaaa atggctgact tgtccagctg gtaaagcaaa
     4321 aattcgtatc tttgtaggac atacatcagg gctaccagcg atgtttatcg agggagacga
     4381 agtaaaatca gatattggct ggacagcaag taattttatt gaagaatatc cagctggctg
     4441 gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa atctattatc aaaaagaatg
     4501 cgtccatcca gtcactgaaa cggagattgg cggaggaatc ttattcgatt ttgggcgagc
     4561 agtgaatggc tgtgtagaac taatatctct ggatgaccat atcgaatcta tcactctatg
     4621 ttatggagag tccgatgtag aagctatgga tatcgattat tgctactata agcaaatagg
     4681 aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta taccagaggt
     4741 ttctactgga atggttcaac ttcaagtttt tcatgaattt ttatctcttt cgaaacaagc
     4801 tgctttttca agcgattctt ctttactgaa tcagatctgg tcagtatcag aagaagcctt
     4861 caagttatgt agtgatttgt tttttatcga cggtatcaaa cgagatcgct ggatttggtc
     4921 tggagatgcg tatcaatcat tcatgatcaa tcaatatctt ttttttgacg aggaaatcaa
     4981 taaacgaacg atattagcat taagaggaca aaatgaaatc aagcagcata tgaatactat
     5041 cgttgactat tcgatcttgt ggttgattgg tatcgaaaac cactatatga tgacgaaaga
     5101 ctatgaattt ttacgcatcg tctaccctaa gatgcaatca ttaatgagat acttgatgga
     5161 gcaaacgaat gaattagggt ttatttatgg gagagcaaaa gattggatat ttattgattg
     5221 gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa gtcttattac tgaaagccta
     5281 tcattccatg atcacttgcg gagatatctt agctgaaaca tatggagaga acgaaaatta
     5341 tcatactcat ctctatcaag aaaaatatca actattgaaa caaaacctat ttgactactt
     5401 ttgggatgaa gaacttggcg cttttatcga ttgctatgaa tctgggagaa gaaatgtgac
     5461 gcgtcatgcg aatattttcg ctgttttatt cgattttgct aacaaagacc aacagcaatc
     5521 gatcttaaag aatgtcttat tgaatgacac tatcacacag attactactc catatttcaa
     5581 attctatgaa caagatgctt tatgcaaact cggacagacg aatatcgtct accaaacgat
     5641 tcttgattat tggggaggga tgttatctca tgatgcggta actttctggg aagaatacga
     5701 ccctactcaa acaggaaatg aaaagtacag tatgtatgga gatccttatg gaaaaagctt
     5761 atgccatgca tggggagcaa gcccaatcta tcttttagga cgctatttta tcggtttgta
     5821 tccaacaaag ccaggttatg aagaatttat gatacaacct aatctagatt catttgaaac
     5881 actgaattgt acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa caaaagataa
     5941 gatcgtgatt acttctgata gaacaggcgg aacagtgaaa atgaatggta aaagttatcc
     6001 tcttctagca aaacagccac ttgtcttaac aagctattca aaaataaatg cataaaacaa
     6061 aaaactgctt gcgacatatc agaagacttt aaatcttctt ctgatattgt ccaagcaatt
     6121 tttattttga agtttgctta attctgttct ttagaaacaa tgtttgttct ttttccggca
     6181 aaaagaaagc tagataatac gacaagtaaa aagaaagcga tgattccaac tgtcaaggct
     6241 atttgaccgt attctcctag tttcccaagt acaagtcctg gtactagata atcagtatcg
     6301 gcaaatgtag aagaagcgat tttaagatct cctaatactg gaaggataaa taatggcagc
     6361 caactaatca ataagctatt gaaagcagaa cctaataccg cccctcttct accgccagtc
     6421 gagttgccga agacaccaga agcagctccg cagaagaaat gtcctactac cccaggaatg
     6481 ataactgtag tttgcaagcc tatcatagca aacatggata gtgttcctac aaagaagcta
     6541 acaaaaaagc caattaatac agcattaggt gcatatggaa aaacgatagg aacatctaat
     6601 gctggtttag aatcgggtac gagtttttta gcaatccctt ggaatgctgg aacgatttct
     6661 gccaacacca ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa tgttccagct
     6721 tggatcagtg caaagattac agcgttgtct ccattgctta gttctctttc aacaaatcca
     6781 ggaccagcta acaaagcaag tgcgacatat acgatcgaca tcaagagcat gatcgataca
     6841 gtagaatctc gcaaaaagct gaaactttta ggaatcttta tgtcctctgt agaacggtct
     6901 ttgtttccaa aaaactttcc gatccatcca ctagaagcat aaccgatatt tccggtatgc
     6961 cctaacgcaa cttgattgtt tccagtgatg ttcttcataa acggttgaca aagagcaggc
     7021 gtaatagtta atagtgttcc ttcgaagacc ccgcctaata agatcatcca caaatttgca
     7081 gtaaaccccg cgcttactaa aatgactgct gttagacaag aaacataaag cattgcttga
     7141 cctgtaagaa aaatgaattt atatcttgtg aatctagcaa gtaaaatatt cacgatcatc
     7201 cctactagca tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat cgccactact
     7261 gcttcattgc taggaactac tccttgagta tgcagagctg tttggaacat cgaagcaaaa
     7321 ggggttagtg aattactgat taaaccagca ccgccgatca gtactaggaa tccgacaaat
     7381 gttttgatag ttccttggat cacttctgaa aatttcttct tctgaagaac gagaccaagc
     7441 agtgcaatca aagaaacgag gatggagggc tgactgacga ttgacacaaa caaattcaac
     7501 aatttatcca ttgatagttc ctcccttgaa tcatttttat tcttttgaca agatcttcgt
     7561 tactttttct tttacttctt cgctgtcaat gatcgattgc aaaggtacga gcttttcatc
     7621 aggtatagaa gttagcgtat ctttcaaaga atgctcaatg aagaaataat cagccatatc
     7681 gctagataca cttccaacat ccgtatgact tacttcaaac tcgtccacat ctgctccaag
     7741 atctgcaagt gcttgtttga tgttcatctc taccataaaa cttgttccta atccactttg
     7801 acatactgca aaaattttca tatcattcgt ctccttttct tatgatcgct aatatttctt
     7861 cttttgttga tgcgtgtaat aaatcttgaa gattttgttt attagaaagt agctttgtta
     7921 aatttgccaa agcccgaaga tgtgcctcat tatcgatagc agctaaacag atgaacaaac
     7981 taatgggatg ggctggatta tcaagcagat ccacaggttg ttgaagtttc agcaaagaca
     8041 tccctacttc gtttacgcca tcttctggac gagcatgagg taatgcgatg tgatcgccaa
     8101 tatggataaa cgcaccatac tgctccacgc gctcgatcat agctttgata taactttctt
     8161 ctattttatt ttctgataat agcggctgtg cggcaaatgt gattgcttct tgccagtttt
     8221 ttggttgatc cgataattgg atcatttctt ctgttaataa ttcagatagc ataggacgtt
     8281 gatcctcctt cttttctaaa agtttgttca ttttacgatt taatacttga tagagctttt
     8341 cttcgtctac gccttctttt aattcgatat aaggcagtag cgccgacaac aattcttggg
     8401 cagaaggcat tgaaaatcct ggtatcagcc aatctctttg tacttgattg atcagctgat
     8461 ttttttccaa gtcagtcatg attggatgca gaacatagag agactttttc ttatcagtaa
     8521 gtgtgagagg tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc tcgtgtaagc
     8581 ggtcagctga attagtctct gtaaaaaaga tcatcgggaa taattgctga agttcacttt
     8641 ttaagatcaa tgaagagcta atgccacttg gacagacgat cacggctctt atttttctat
     8701 ttctttcttc tgcgtccgct tttctgatct ctccgccaaa tagtatagtg aaaaaaccaa
     8761 tctcttcttc agggatgctc ttccctattc gtctttctaa cggaagtaat gcttttcttg
     8821 tcatttcgta gatggaagca tattgatgct taatttgatc gatcatcaca ttaggtaaat
     8881 aaaaagaata ggaaatacga aaataggcag ggaccaagtg ataaaagaca ttcatcaata
     8941 attcacgaaa cgaagcaaat tctacggcca tcaggaattc catgcgatgg atgacttctg
     9001 aagaacattc cagcaaaaaa tcaagtgcag gctcctggac atctccttca acgatcgatg
     9061 ctaagcaaag agagaaaaat ataatctccg aattttgatg ttcggaatag ccaaggccag
     9121 tcaatacttg tcgactaatg tcagtgactg caagagaagc caacagttca gcatctttta
     9181 ccagttccat ggatgttttt ccaattcgaa tggttgtcaa actcaaataa taagctaatt
     9241 catccattcg atttggtgcg aatcgaagat ctgatagtga aatcgtttta tctagcaaat
     9301 ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg agcccaaatc
     9361 ttccagcaag tgactgcatc aaacgagcag tgcgattttt tgcttctctt cttaatattg
     9421 cttctgaccc tagcagccta aaaccagttt tcctagtata cgacagctga caatcttcca
     9481 cttctaattc atttcttatc tttttgatat ctgacaggac tgtcccttta gaaacctgaa
     9541 gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt cgaataaacc atcaaataaa
     9601 gaagatctct tcgctcttgt tcggtataca cagcttcaaa tcgagtaata tccaaaaatt
     9661 tttctgccca gtcgattgct cggaatttct ttatacggat acgggtatca tccacatatt
     9721 cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt tacttcgatg atctctgctg
     9781 ctttttctag actgatttct cttggtgttt tcaggagata actaattttg tagttcacag
     9841 cgctgttcac ctcctactta tattctagta tggtagcgct tttaaatcga ggggtgtttg
     9901 gtacaactta cagttggatt ttttttacga ctgtaccgaa cgatttctat cgaatggata
     9961 cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg aattgatttc gtccgatcaa
    10021 tgatgatcat gtttattttt tgaggtcagt tcttcaaatt tttctttgac atcttcaacc
    10081 gctttttcga tatctttttc aaagtgattg ggctcattag gctttgtttc ctcttctaca
    10141 gaatttgaac cagataaagc tgtttcttta ttttctgttt caccagagtt tgtgccagct
    10201 aatgattctt ctttgtcttc tgtttcacca gaattagttc ctgctaaagc cacttcttct
    10261 tctgttttag gggccattga acctcttaat cgtttgtgtt tttcaccgtc ataatttgtc
    10321 atgttggttc ctcctagtag ttctatactt tttcatcgta caataagaca gtttatgcgt
    10381 caaaaaacaa acccgattct tacaaatatg tcatattttt ccttctgatt cttgaatgct
    10441 tttgattcct tcttccaaaa ttttcatttg taagattgtt tcttcatctt tgatgacttt
    10501 ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag acacggctgt aatcaccaac
    10561 ctcggaaatt actttttctt catgataact cccttgttca tctatatagg tcaatacacc
    10621 ataatgttcg ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa acattttcag
    10681 atgttcttct tgtcgatctt ttgtttgttt cacgaacatc cctttttttc cgtaaacgac
    10741 gaaacttgga cgttctttga tgcggaaata actggatttt atcgaaattt tcatatttcc
    10801 atagaaaaaa tccagatcga agtaatcatt cattcgtcct ttccctaaaa gttgacgtac
    10861 atcatagctg atatcatctg gttcgccgaa ataagaaaga acttgatcta ctgtatgaca
    10921 agcatgtcca tataaatagc tgttcgcagt agaaaattca tgcgtatttt ctggtacttc
    10981 cggtctgaaa taatcataat gcatttctac ttctaaaata tcccctaatt ttccgctttc
    11041 gattactttt tggactgtca agaagtcaga atcgaaccgt ctgttttggt aacattggat
    11101 aaatagacct ttttcttttg ctaatgcaaa taactcttct gcttcctttg acgtttctgc
    11161 aaaaggtttc tctactagta cattcttacc atgttcgagt gccatttttc catattcata
    11221 atgaaattga ctaggtgtag tgatgacaac taattggatc tcaggatcat cccaaagatc
    11281 agacagctga tcagtatatt cgatgtcttc gaatgttttc cacactggtt cagatgggga
    11341 aaaaattttt ttgatattga ttttgttcgt aagttttctt gaaaaaggca aatgatagcg
    11401 atttgtactc tttccattgc caagataagc gattgtaagc atgagtggtg cctcctttta
    11461 ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat ttataatagc tattctggta
    11521 aaaaagttca gaaaaacact tgttatcatt cggtttggtg aaattaatgt tcaaagtaag
    11581 gataaaaatc aaaaagatag tagcggaacc aagtgtttat cagtaagata gaatttggag
    11641 gtcagatcat gcttattgaa gaaaaactaa caaaacaaga gttgtttacc acgacagaga
    11701 aacggattgt cgattacatc cgtaggaata tcgaagctgc agtctacatg acgattgaag
    11761 aattagcaaa agctacgtat acttcccatt cagctatcat tcgtttatgc aaaaaaatgg
    11821 gattcagcgg atttaaagaa ttccgtttcg agctggggcg ggaagttcat caattgattg
    11881 ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc aagtgaagat agcactcagc
    11941 aaattgcaaa gaaaatggcg gatttatcga tccagtcaat aaaaaaagca caattgcaga
    12001 ttgaacatga agatttagat caaatcggta aagtattgaa caaggcacag cgaatctttc
    12061 tttttgcaaa aggtgattct caaatcacgg caaggaaatt ccagaataaa atggtcaagt
    12121 tgaataagtt tttgatcatg gctgaagaat acagtgattc ttcttggaat gcagccaatt
    12181 tgactagcga ggattgcgcc atttttatta gctacagcgg tcgcatccat cattatgaac
    12241 gaataatgac ctatttgaag catgtagagg cccctacctt gctgattacc ggaaatcagc
    12301 attcagaaat ggccaaacag gctgatatgt gtcttgttat atcgcaggag gaatatgatt
    12361 ttgctaaagt agcgactttc tcctctcaaa ttgccttcga ttacgtatta aatacgttat
    12421 tctcagtgat atattctcaa aattttgaag agaatatccg caacttgaaa aacaaacaag
    12481 aattgatgca aaaaggcctt cttaaagaat atcggaaaac ttaagaaaag atcctcattt
    12541 agtaataaga aagatagtgt ttgataggct ctctttgata tcatcataaa aacaggctga
    12601 agttttcttt ttcaaacctc agcctgtttg tttacggaac gattcgaata gatatattat
    12661 agaaatgagg aatagctaac ttctgtttct tattttgaac gattgacgaa aaagatatat
    12721 aagaaggagg aaaatcagta tacatgcagc taacaatcca tacaggaaaa atcgtttgtt
    12781 ctcggatact ttctgtactt ctttttgaac ttcttttgca tgataaggaa cccggtgccc
    12841 tctgacaagt aagcgatgac tgttgaccat ataaggtgta caagttagga gtgtcacgaa
    12901 atcctgccct ttttcgattc tcagatcttt tgtatcagtc ggttctaccg tcttgatctg
    12961 atcgacttga taagcgagtg tccgtccatt gatttcgata tagaaagaat cacctaaagt
    13021 cagtttcggt aagtctgtga acaactttgc ttgaggcagt cctcgatggc tggaaataac
    13081 ggcatgtgta ttttctcccc ccgtaggata agacgttcct tccaataatg aggcaccttt
    13141 ttctagcagc acagtattcg tttcatcaaa aattggcaaa tggacattga tctcagggat
    13201 cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta tcctttttcg caggttcttt
    13261 tttcttagta aagggatctg cacctggatt tccaccttct tttgctaatt gctggttttt
    13321 ctttgtcatg ttttcttgga ttttagccat gacttcttca ttttctttga ccgcttgttg
    13381 ctgataatga gagatgatct gctgatccag ataattattc agcgcgtcgc taacaaatgg
    13441 ataagctagt atccctattc ctgagagaag caagaaaagc atcagcagat ctagaagttt
    13501 ccttttcttt ggtttcattt gtgttctcct tgtgatgact gtttgataat aaaagaaaaa
    13561 ggagcatttg cgtaatccgc tgctcctttt tccaattctt gcaaaaagac taaatctgac
    13621 tgtgcttgcg tctagcaaag tacagtccag caatcagtag aaggactact cctgcaccga
    13681 tatacacata gattccctta ccgcctgttg aaggaagtgt acctttgtgt ttatttggta
    13741 ttttttcagg agaaatcaac tgtcctgctg ttacataaga ttgttcatcg atagtaaatg
    13801 ctacacggtt tgttaatgga acatattttt ctggcgcttt cgtttcttcc agatagtacg
    13861 tgccatattt tagacctgtc acatcgatta aaccgttact tgtggttgta aaaaccgttg
    13921 ctgattcttt cgccgatacc cagctgacgg cttttgtgga tgggtcgatc gataaatatt
    13981 tcgctgtgtc actatcttga tcacgaacga cgaattctgc tccagcaagt gtttggtctg
    14041 atgtaacgtc accatctact ttaacaaatc gtttgccccc agtaacgaca tcgactgacg
    14101 gtggtgtttg atcatttgta tgcccgttat cgacatttgc ttggttagaa aatcctttgg
    14161 ttggatctgc tttttcgttc aaatgcatat agtaaacgaa tttcaatgta ccgccaggag
    14221 ttaatgaagg aatataattc ggatcaactg aaaccgtgaa tccgtctgtt tgctcagtga
    14281 cagaataatt tactgggtcg atttctttat ttccatcata taaggcataa gcatacgttc
    14341 ctgaagaatc attatcaaat gttaaagcag cgtcatgagt atcgataagt ttgaatgttg
    14401 tgtacttgtt ttgcgttcct tctttatcag cgatacctaa tgggatattg acagaaatct
    14461 catattgaat tttttcaccg attgcgacat cttttccatt caattgaggt gttgttttat
    14521 caactttaga cgtatcattt ttgatggtct tttctactgg tgtttggcta tttgccagga
    14581 tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc attatcagga gcttttactt
    14641 cttctaaatt atattttcca acttctaaat gattaacgat caactggcct ttttcaatag
    14701 atgcctcggc aaagtcattg ttgccgatgt cataagaatg accagtaatg aaatgtttgg
    14761 ctttggtttg atcagttgtc caagtgtaca atccatctgt gacactttgg atgtattttt
    14821 tgacgcttgg tgttccttct tctttagaaa taataaattc tgctccattt agtgcttcgt
    14881 tttcggcagt accgattttt gtaactttca acgttccatc attaccgact gtatttttag
    14941 ggtagagatg gatagtatct agttcttctg tcccgtattt ataagagcca tctgcttgtt
    15001 tgatcatctc atatacaggg aaagctaaaa ccatgtttgc ggcagctgac actccgtctt
    15061 ttggttcttc tttgatcgtg tagactgcat ctttcccatt ttgtttttta ggtaaagata
    15121 aagtgacatt tccatcagca tctgtcgttc ctgaagcaac tggtgtacca ggagtcaaag
    15181 actggactgc ttgttttgct gcatccacgg acgctccttt atctcgttgc gcataaaatt
    15241 cttgagtgac gttataaact gaaaatgaaa tatcggctaa tccttggtat tgatcgaatt
    15301 cgctcatttc tttcccgctg ttttggatta aaggatcggg taaatcagtc attttctttt
    15361 tatgaaggat gacttgagct gctgtctcct ctgcaaacac tttttttgcg ccgaagctgt
    15421 ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat aacgtttatt tttttatgat
    15481 ttttcatttt ttcactcctg ttcttttttt gataaacaac cagattgcaa agcttgcgaa
    15541 taccatacca acaacgccta aaagtgtatt tttcgtttcg tttgtttttg gaagaaatgg
    15601 acggctattt tcttttttat tgactacttt caatatactg tccgcctctt tatcctcaga
    15661 aagaaaagtt gaaatggtaa acggaatttc tgtttcactt aaaatataac cagaaggtgc
    15721 ttcgatctct tttaatcgat aacttcctgt tttcaacccg gaaattgaaa aagcgccatt
    15781 tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt gtccacttgt atccgttggc
    15841 tgtttgctgg aggtaatttc ctgctttgtt tttaataagg aatactgcgc cagctaatcg
    15901 tttatctggt ttatttgtct cgattttgac aaattgctta ctccctgtat atacagagac
    15961 tttctttgta ctgacaccaa aaccaaaatc cagtgttccg ttgttgttga tttccttgtt
    16021 cgcctgtgct gtactgctta gctgcatctg ataagaaatg gtcagttttt ttccagcata
    16081 tttttccaac gaagggatcg aaaataaaac atcaaatccg tgaggtttct tgtgtatgac
    16141 aaagccttct gtcagctttt ctccattcga tgaaatcgtt aaactattag gtaaaaacgt
    16201 taatgcttca tccgcactat ctgacaattc gaacttttga tagtcaagga tatttacagg
    16261 aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc gtaaaatcat ttctaggctc
    16321 ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt tcttcatttt taggatacaa
    16381 atggatagtt gaaagttttt gattgttttg tccatgtaca ggaagaacaa ctaccatatt
    16441 ctctgccttt tctttgacga cttctggtac tttggattca atgaaaagat acgctttatc
    16501 tcttttttct tgatctttag cggccagaga aaaagaagcg ataccatctt cgttgttaag
    16561 agttgtagtt gttgcttctg cggtaaacat accggaagac gcaccgtttt ttgcgatctc
    16621 tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga tggtaaaaag aatctgtgac
    16681 atcataaact tggaatgtga caccatttaa tcctcgatac gtttgtaata aagctttttc
    16741 ttgtccgtca tttggatgat ttttcggcag ttgcccgttt gggaaaagca atttgtgtaa
    16801 agtaatctga accatttctg tttctgtttc tgttgctacc tgagtaaaag ctggtttaaa
    16861 tagtaacaag aagagacaca tactaagcca accaagtttt ttcatattta gctgccccct
    16921 ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac aaccaatcat ccctactaga
    16981 tagattccta aacggccaat tcctcccgtt tcaggtaatg gtacttttgc ctgattcgtg
    17041 atgtctaaag aaatctggtt gtttttggct cctggtgaca gaacagattc atgatcttgt
    17101 ccatccactt gaattgaccc atcttcgtgt ataactatag taactggctc ttttagacct
    17161 tggtatcctt ctggtgtaaa agtttcggtc agcgtataag ttccaggttt taaattttcg
    17221 aatagaaagg tatctgtttc ttgtccatct tttggcgatt ctaagtccat ttctggtccc
    17281 tgcagtctga attttgctcc ttttaatgtc tgtccgttgt cttctttttt gtgtaccgtt
    17341 agatcaaacg cttttagtcg attttgatga gtgagcgtcc atacattatt tgtcatctcg
    17401 gtattttttc ccgtgtaaat gatttttcca tatttatcga tttggaattc ataatttccc
    17461 ggcgaagtat catatccggc aggaccagct gtttcaacga ctcgatattt accaggtgtg
    17521 agtgaatcaa aactggcaag acctttttca ttcgttgttt ggctgtcaat tggctgataa
    17581 gtaccatttg cttctttttt ctgtagggaa aatgctgctc ctttaagatt tatctctttg
    17641 tccgtccctt gcatcgcata cttacgtact gctacaggaa cttcaacaaa aggattttca
    17701 atagtcaact ggatcgtatc atcggaagta gtgactgttt ttccatcaat ggttaccgta
    17761 ccatcagaag cgatcttgat ttcccaagta gtctttttgc ttaatccatg cccttctgga
    17821 gcttttgttt ctgtcagcgt ataggtcatt tctttttgca atttgacatt ttctggaaga
    17881 gaataggttc cgtcgccatg atctgttagt aaagtatcaa tagaatctcc tgttaatttg
    17941 aaaacagcgc caataagatc cttttcacct gtagaggaat ttttcgttat tttcaagttt
    18001 aacggtgtga attgtttagt atttttccat gtcattgcat ctatttgttg agaatcgtat
    18061 cctggtacag gtaattcttt gattgtttgg taactgaatg cttgaccttg attattgtat
    18121 tgaggtagtg ataatatctc ttgataactt tctccgctat ttgcagataa tttgtcaatg
    18181 tctgcacgtt cccacgtatt tgttgtatct ttagctggtt taatgattcg aatatatccg
    18241 tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa tctcaaaagt aacttgatct
    18301 ggacgatcag ctagattgtt gtcaaattct tcccataact ttttgatgtg aagactgact
    18361 ccgggagctt tagcagatgg tataccgaat tcagctaatt cattggtatc aatgtttggc
    18421 tggaaagttg tcctgccgtt catttgatac cagaaatttg gatggaagtc ctcattttct
    18481 gtttggattc tcacttggta atggatttgg atttcttggt cttttcctaa atagatctga
    18541 ttgctcttga tggtatttcc ttctatggaa atagatggag tgaccgttgt aggacttgtc
    18601 cccacactct tgactgaaag tgtaccaggc tgataaatga atggttctgc gattggatca
    18661 tttatttgtc cattgcttac agttgctgag atctgtacag cttttttggc taggtattca
    18721 gagatatctg ttgcatgatc agctgattca tagtaaagat cgcctttttc atctgatgaa
    18781 accatttgtc gcatacgaga ctctacttct gcttttgaga gaccagctgc cggatcgctt
    18841 tgaagttgga tgccaagacc atgtatttcg attcctcgtt ctttgagtgc catcgcttct
    18901 ccgatggttg cgataaacgt actgtcgatt cttctggata gattgttttg gtcaggtgca
    18961 tcatagattc ttgagattag agaagtattt cccggccgat cttgcgtatt agaaaactga
    19021 gttccgtaat aattgctgct tgattgtgcg tgtacccgct gtactttata ggaaaatgtt
    19081 ggtacaccat ccgtcagcaa aacgatcact tttttatgtc catttggaac ggatagcatg
    19141 cttcctgcat ctcttagtgc tttttgtgta aaagtaccac catttgtccg tgaaggtgta
    19201 atggatttta cttgattttt cactgaatca aatgaaccca tctgtactgc accgttactg
    19261 tagctatatc cttcgcttga gtagccgaca tatcccatat tgattttgtc tgtgataccg
    19321 ctatctgcta aagtatcgac aaaacgatcg acaccaatct ttacttcacc gatccgatta
    19381 ttgtcgttca tacttcctga ccagtctacg accaatacga gatcaagagg cgtgatatct
    19441 ttttgtacat ttccacgagc attcaaataa acatcaaaca atccgggtgt acttgtttcc
    19501 tttgcatact ttcgtatcgc atagtctgct tgattaccgg ttcctccata ttcgatatac
    19561 gaatgggtcc gatcattagg atctccattc caagaattta taccatccca ttgatttgtt
    19621 ccgtttttat tgccttgatg atcaagaaca tttgtatttc ctgtaggttg ccaactggct
    19681 ttaggataag tgccctgatc atctgttgta tattctgtag gaatcgtttg attagatggc
    19741 acactcacag ctggcaagga taatgtaggc aatgtaaatg ggctgacaga attttcgttt
    19801 tcttttgatg attcttcttc tatttcgtct ggctttggta ctgacacatc tgtatctttc
    19861 ttattttctg tctcatcaat aatgtcatat tctttattat cgacgatcag cttgtttgcc
    19921 tggctgtggt aaagtgctgg atcgattgga aaactcagat cgatagtttg tttagtgttt
    19981 gatgagattt ccaacgtaat tttcccgcta tttacctgat aagcatagcc atctccttct
    20041 tttctttcca catcacttaa gtaattttcg actagaccaa tttctttttc tatcttttca
    20101 gcactggtgt tattcgcatt tagcttgatg ttcagtgctt tttttccaga ctgatccgaa
    20161 atgacttcag ctgaaattgt ttctaaaact gttggatcat ctgtagtgtc tgctattgca
    20221 gataatggca caaagtttga taaactcaat attatcagca tgaaaataac tttcagtttc
    20281 ttccctgtgg tcgtcatttt tcccctcctt tttctttttt tctgttttga cgtgaatagt
    20341 gaaaattatt aaaataaaca ggcgttacat aattttattt attttaatat gataattatt
    20401 atacgaaggg cgctaaaggg aatcacacca attttcatac caattggttt catttatgaa
    20461 gttgattcct tcgattaatg agcgtttcat tgaaagaaaa gggttgaggg agaaaaacat
    20521 atgatcaggt tgttggaagg aaactataga aagatggaaa aaatcattcg aattctgtcc
    20581 atttctgaca agtggatgac taaaaaagaa cttgccaaac gaattggtag ttcagaatct
    20641 acatttgtaa ggtatatcga agagatcaaa atccgctggg gaacggatgt aacgattcaa
    20701 tcttcacaaa aattggggta tcgtcttgaa catttgaatg taccagctta tttaaatgtt
    20761 ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat taaatgagat tattgtcaat
    20821 ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca tcagccggtc ttcttttaca
    20881 cgtaagttga aacaatgcaa tcaaatttta gaaagctatt tattaaaagt gatcgttgac
    20941 caaggttttc agttaactag ccttaaaagt gagctccagt tgcgtatctt tgttaccttt
    21001 ttctttttaa gttactacgg aagacgcacg ttgccatttc caatcgaaaa aaagaagatc
    21061 aaagaaatta tggtcaagaa taattgtcag atgaatttgt ttcaggaaga aagcccatat
    21121 gagcaatctt tctttattat gtattttgtt gtgcatctca tgagagaatc ccaaggaact
    21181 ccatttgaat ggaaagctgg agactcttat gtacagttga taaatatcga taaaaaagac
    21241 ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat aaaagtagtg
    21301 aactattttg tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa tgaaatcagt
    21361 gagcagatac gaaaaaatat gtcctactgt tcgttttcat ccgctgattg caaagaaaaa
    21421 aacgaagaat atgttgttca attattaacg caaatttatt ttttctcccg tattttccca
    21481 tacgatacga ctcatttgac gatccgtgca acttactttg cacagcagat gcaacaccgc
    21541 cagatacgtc tatatgctgg atttcgtgca gaacttcaat tttattccac tctactgaat
    21601 cagaatctga tttattattt atctagctat atcttttgga tgcttatcgg taatcctgaa
    21661 attcaccatt atacttctga taaaaaaata ctgattatca gcgacttaag tcttcgacat
    21721 agtcaatata ttgaagaata tatcagtgat attcttgctg tgcataaaat tcactctgag
    21781 acaactgcta ttactgaaga tcagctgtcg aaatacaatc ttctcgagta tgatctgatc
    21841 gttacgaatc aacccatttt gaatgcccat gttcctcata tattgattga tgatagtgtg
    21901 tcattcgcaa atgaagaaga actcatccgc ttattcgagt tatgaataat caaaaaacag
    21961 ctatgtataa accaccctga taaaaatttc aggacaaata cacatagctg tttcttttta
    22021 ttgtattcct tcagcaatcc aacgagattt atagcgaaag tcttttaaca actcttcttt
    22081 tgtccaatta tttgcaatga gatccgtcaa agtgttcatt acgagggtca tggaaaagta
    22141 taggaaacta actgcttctt ccgacgattg aatggagaaa tgattgattc ttaataattc
    22201 cagccatttt tcgttcattg gagaatcagc cgatagttct ctacgtttgg ttgtctgctg
    22261 atcatttcgc tgggttaaca agtataacct ttgccaatag atactttcgt gatctaagtc
    22321 gcagcaccac tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat
    22381 gaattttaag atatcttgat ggactatgtg agaactgctt ttgatcgtat acaaataagc
    22441 atcttccaaa tcttgaaaat atttataaaa agcaccgcgc gacatcccca tcttgtcaac
    22501 gatttctgag actttgactt ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc
    22561 atcgattctc tgtttctttg cgttcgataa attgaaaaaa gtttcttttg gcatccacat
    22621 ctctccttta tctctgatta tacgtggata gcccttcatt gtcatgaagg attcattcct
    22681 tctttttctc ttcttctgtc aagtacccat cttccattcg ataaacgcga tcactccatt
    22741 gaatcatacg gacatcatga gtaaccataa tagtcgcttt ttgtttctca tgagcttctt
    22801 taactagtaa tttgacgact tcatatgcat gatctgtatc caaactagct gttggttcgt
    22861 ctgctaagat caagcttggc tcattatata aagcgcgagc aattgccact ctctgtcttt
    22921 ctccaccaga aaggtcttta ggataactat ttttaagtga ctggatatct aaagattgca
    22981 gcaaggaatc gatttttgtt gaatcttctt tttttgaagt aacttggtca actagttcaa
    23041 attgttcctt taccgttaaa aaaggaatca aattagagct ttggagaata aatcctattt
    23101 ctttaaagcg taaatctgct cgtttttttt ctggaagctc agttagtgag tgaccgttga
    23161 tcactatttc tccggaagtc ggtgtttgca atcctcctga aatggtaagg aaagtacttt
    23221 tacctgaacc agaaggacca atgatgctga cgaattctcc ttcctttaca taaaaatcaa
    23281 ttgctttcaa agctgagacc tttgtatgac cagaaccaaa tgttttggta acttttttca
    23341 tcgttaatat ttttttcatt tttattaacc tcctattgct gtaattgggt ctactttggt
    23401 tatcgtacgt atcgaaaaca atccgcctag cattgcgaca atgatcaaaa tcccactata
    23461 tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt
    23521 caaataagca aacaaaagag caagcagaac gcctaccacc ccaacaataa aggactgtgc
    23581 tactaaagat ttagcgatga agaaattttt gatcccttgt gctttcatta ctccaaaaat
    23641 agcagtcttc tgaagtgtca tcacgtacat gaaaataccg accacggcag caaccactaa
    23701 gaacaaaaag tatatcatcg catttaaggt catattttgg gcagaatagc caggaatact
    23761 ttcaatgaat gtcccaatag ttaataactg tagacccttc gatgcttttt ctttcgaaat
    23821 tttagctgaa tcctttatta cgatcccatt gatcggctgt tcttcttcag aagcaaacgg
    23881 ctgttgtcca tacttcaaag cagtccacgt atctagggac gcatagatga cgggagaaac
    23941 tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt aactgccaat
    24001 tttgatcgta tctccaattt tatagcctga atcagccaga ttttgagaga tgatgatttc
    24061 attctttgtt ttaaattcat gccctttcgt gagttttggt aataaaaatg cttctttcgt
    24121 tgtgccgaaa actgtaacat tttctttgtt ttttcctttg attgctccgc tgtacaatcc
    24181 gattggtgct ttctctcctc cttcaatgta tttcagatct cctcttgtta attgtgaagc
    24241 tgcaaatact tgatttgcat cctccgacaa aacaatctct tgtgcgtccc aatcatcgat
    24301 tgctttttta aactcttctg aaagtccatt cgctagtcct gacaacatga atacgacata
    24361 cgcaatcaag aacataatac cgacgatcaa gccatatcgt aatttagaat agcgaatttc
    24421 tttgatagct agaaacattt gtttttcctc cctatgatag attgatagtg acaaaatgtc
    24481 acttgttgaa tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat
    24541 caataaattg tctaagacat ctttcgataa aaccttaaca tgagtcataa aaaatttatt
    24601 taagccttat ttatgctttc catgataaac taaagtgacc aaatcaatga ggtgggaaaa
    24661 tgattaaact attagtagta gaagacgata aagtattatc caataatata aaagaaatac
    24721 tcgctgcaat cggcgagata acacaagttt atgatggtag tgaaggactg ttcgagttgg
    24781 aaagtggagt ttatgatctt gcaatcttgg atttgatgat gccggaaatg aatggatatg
    24841 aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc agtattgatt ttgactgcaa
    24901 aagatagttt ggaagataag attgaaggat ttgaacgagg tgcagatgat tatctaacaa
    24961 aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag
    25021 gtctctttga tgaaaatgtt ctgaagtata aggatatcag ttgtgattta aaaagcaatg
    25081 aagtcttgta taaagaacaa gttttgccca tacaaggaaa agagtttgaa ttgctcgttt
    25141 attttttaca aaataaggga atcattttga caaaagagca gatatttgac cggatctggg
    25201 gatttgattc tgatacgacg attacagtta ttgaagttta tatgagcaac ttacgaaaac
    25261 atctgcgaag tactggattg gataaagaaa tcaaaacctt acgtaatgtc ggttatatac
    25321 tgcaaagggt gtgatgcaat gaatggagat attacacgaa agcaacgtag tcgctttttt
    25381 gcgacaaatg tagcagcatt tgcttttatc ttttttcttt tgggattcat tactttacag
    25441 atattaaacc aatctgcata cagagaaaca gatctgtcat tagagaaaat aaattttgat
    25501 tctcgaacag tacaattaga gatacaaaga tatcagcaag gcgatccctt tttaaacccg
    25561 actcaaacgg aacaaaacag aacaaacatg gaacagccat taatggaacc atcaaatgac
    25621 cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg agatattgaa taaagcagct
    25681 attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc
    25741 atccaaaata ttgaattaaa tgaagacagt ggctcatctg ccctctcttt ccgttcgatt
    25801 acgaaaaaag cggagacgaa tcaagatggc gttgcctaca tccaaatttt agaaaataca
    25861 aatcaaatca agaattcatt agcgactttt cgcacgatta tgattttgtg tatgattgtt
    25921 ttttggattc tctctattgg tatcagttat tacttgtcta gtctaagcat gcgccctatt
    25981 attctttctt ggagaagaca aaaagaattt gttgagaatg cttctcatga attgcgcaca
    26041 ccgttaacga ttattcaaaa tagtttggaa catcttttta ccaagcctaa tcatactatt
    26101 attgaagaat ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct cactggattg
    26161 acttcggatt tgctgacaat cgctcggaat gattctgatc agcagctgct ttcaaagcaa
    26221 atgatcaata cacaagaata tatcgaacaa cttgttaaac catttcaaga gatggccatc
    26281 atagatggaa aagagtttat tctccataac gatgcaacat tagaagtacc aattgatgaa
    26341 aaaaaaatcc atcaagtttt ggttattttg ctggataatg cattaaaata tacgaatcca
    26401 gaagataaga tcattttgga ttcatctgtt aaccataaag aatggctgat tcacgtaaag
    26461 aatactggcc cttctatcag tgaagaagat caagcaagga tttttgatcg attttatcgg
    26521 gaagatcaat ctcgggcgaa agagactggc ggatacgggc ttggcttggc aattgcaaaa
    26581 caggtaatcg aacagcatca tggacagatt tctgtatcca attatcttcc acaaggagtc
    26641 gattttaaag tccaattgcc gttgaagcct aaaaaataat gcttatccct aaaaaagggc
    26701 tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat aaacggtgtt ccagaagtag
    26761 cttcttcgga aataagccga aatttcttta agctgaccac ttctgtcaca acctctttag
    26821 cttacgctta cgatttttta ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat
    26881 ccaattcttc tcggctaaca tcttctgtca ctttttcaac atcttcatgt tcttctttat
    26941 gaatcccaac ttcttcacga ataactgtat gtttgcttac atctacttgc tcttctttga
    27001 ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa gattgatctg
    27061 ttactggttt gcgttcgatg acgatttctt cacgtttgac cggtacttct accgtttcag
    27121 tatcgttgac cacatgtttg tggatgtcga cttctccagt tgtcacatca tgtgtattta
    27181 catctaattt ttcttctttt agttgaattg tatcatcatc tagatttggt ttgcgatcga
    27241 tgtcttcagt cttgcttgta cccattgtat cagtctcgcc aggtacgtcc atcggatcag
    27301 ttcctggtgt catattcaat ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt
    27361 ccattggatc tgttccagtg ccagtttcta atccagtccc cattgtgctt gctgggctaa
    27421 cagtatcttg tgtatccatt gtatcttctg ttgtttctgg ctgacgatag ccttttactg
    27481 tgatgacaaa ttttccatca gaaatatctc ctctatattg atataaagga tcattttctg
    27541 gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt atcttttatt ttttcccata
    27601 aagaacggtc ttcttcacca tccatccgat tttcatctgt ttcaacatct ataccagaca
    27661 atccgtcaaa tcctagtcta gatgctgctt catcagtagt ataaataacg atatcatctc
    27721 gttcataccc ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc ctcgtactat
    27781 agcttccgat gaccgttaaa tttttctctt ccatgtaaat tcctcctagc aaatagattt
    27841 atgattggta tattgttact tataaattta ttctatagga gacgattgtt ttctacaaat
    27901 atttagattg gaatttgatt aaaacaattt aattgaaatt tgaaaacatt tatgttaatg
    27961 aaagtcatat tct
//
LOCUS       DANKAZ010000031        27927 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000031 DANKAZ010000000
VERSION     DANKAZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(112..471)
                     /gene="rplT"
                     /locus_tag="PWY67_001247"
     CDS             complement(112..471)
                     /gene="rplT"
                     /locus_tag="PWY67_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL2057543.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(524..724)
                     /gene="rpmI"
                     /locus_tag="PWY67_001248"
     CDS             complement(524..724)
                     /gene="rpmI"
                     /locus_tag="PWY67_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL2057544.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(796..1320)
                     /gene="infC"
                     /locus_tag="PWY67_001249"
     CDS             complement(796..1320)
                     /gene="infC"
                     /locus_tag="PWY67_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL2057545.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1364..1489)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1640..2272
                     /locus_tag="PWY67_001250"
     CDS             1640..2272
                     /locus_tag="PWY67_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2057546.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2274..3179)
                     /locus_tag="PWY67_001251"
     CDS             complement(2274..3179)
                     /locus_tag="PWY67_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2057547.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3264..3923)
                     /locus_tag="PWY67_001252"
     CDS             complement(3264..3923)
                     /locus_tag="PWY67_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2057548.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4239..5183
                     /gene="mvk"
                     /locus_tag="PWY67_001253"
     CDS             4239..5183
                     /gene="mvk"
                     /locus_tag="PWY67_001253"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL2057549.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5176..6153
                     /gene="mvaD"
                     /locus_tag="PWY67_001254"
     CDS             5176..6153
                     /gene="mvaD"
                     /locus_tag="PWY67_001254"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL2057550.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6166..7251
                     /locus_tag="PWY67_001255"
     CDS             6166..7251
                     /locus_tag="PWY67_001255"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL2057551.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7248..8312
                     /locus_tag="PWY67_001256"
     CDS             7248..8312
                     /locus_tag="PWY67_001256"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL2057552.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8561..9364)
                     /locus_tag="PWY67_001257"
     CDS             complement(8561..9364)
                     /locus_tag="PWY67_001257"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL2057553.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="PWY67_001258"
     CDS             complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="PWY67_001258"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL2057554.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10242..11249)
                     /locus_tag="PWY67_001259"
     CDS             complement(10242..11249)
                     /locus_tag="PWY67_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL2057555.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11256..11900)
                     /locus_tag="PWY67_001260"
     CDS             complement(11256..11900)
                     /locus_tag="PWY67_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL2057556.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12000..12758)
                     /locus_tag="PWY67_001261"
     CDS             complement(12000..12758)
                     /locus_tag="PWY67_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL2057557.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="PWY67_001262"
     CDS             complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="PWY67_001262"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL2057558.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="PWY67_001263"
     CDS             complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="PWY67_001263"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL2057559.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="PWY67_001264"
     CDS             complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="PWY67_001264"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL2057560.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="PWY67_001265"
     CDS             complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="PWY67_001265"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL2057561.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="PWY67_001266"
     CDS             complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="PWY67_001266"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL2057562.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18156..19025
                     /locus_tag="PWY67_001267"
     CDS             18156..19025
                     /locus_tag="PWY67_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL2057563.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19151..20050)
                     /locus_tag="PWY67_001268"
     CDS             complement(19151..20050)
                     /locus_tag="PWY67_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2057564.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20215..21039)
                     /locus_tag="PWY67_001269"
     CDS             complement(20215..21039)
                     /locus_tag="PWY67_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2057565.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21050..22345)
                     /locus_tag="PWY67_001270"
     CDS             complement(21050..22345)
                     /locus_tag="PWY67_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL2057566.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22361..22840)
                     /locus_tag="PWY67_001271"
     CDS             complement(22361..22840)
                     /locus_tag="PWY67_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL2057567.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22846..23874)
                     /locus_tag="PWY67_001272"
     CDS             complement(22846..23874)
                     /locus_tag="PWY67_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL2057568.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23891..24997)
                     /locus_tag="PWY67_001273"
     CDS             complement(23891..24997)
                     /locus_tag="PWY67_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2057569.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="PWY67_001274"
                     /gene_synonym="adhC"
     CDS             complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="PWY67_001274"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL2057570.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7873 a         6131 c         5040 g         8883 t
ORIGIN      
        1 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
       61 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      121 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      181 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      241 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      301 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      361 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
      421 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
      481 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
      541 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
      601 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
      661 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
      721 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
      781 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
      841 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
      901 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
      961 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1021 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1081 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1141 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1201 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1261 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1321 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1381 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     1441 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
     1501 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     1561 gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc tatactaaag
     1621 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     1681 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     1741 gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     1801 acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     1861 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     1921 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     1981 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2041 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2101 caatcagttg accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2161 taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca
     2221 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2281 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2341 attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     2401 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     2461 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     2521 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct
     2581 ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact
     2641 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     2701 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     2761 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     2821 aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg
     2881 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     2941 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3001 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3061 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3121 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3181 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3241 ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat
     3301 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3361 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa
     3421 cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac
     3481 gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact
     3541 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     3601 agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     3661 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     3721 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     3781 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     3841 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     3901 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     3961 aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta
     4021 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4081 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4141 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4201 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4261 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4321 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4381 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     4441 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     4501 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     4561 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     4621 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     4681 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact
     4741 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     4801 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     4861 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac
     4921 cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca
     4981 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5041 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5101 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5161 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5221 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5281 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5341 gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt
     5401 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     5461 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc
     5521 gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga
     5581 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc
     5641 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     5701 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     5761 cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     5821 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     5881 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     5941 tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt
     6001 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6061 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca
     6121 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6181 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca
     6241 gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga
     6301 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6361 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact
     6421 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     6481 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     6541 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc
     6601 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     6661 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     6721 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     6781 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     6841 tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat
     6901 cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     6961 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7021 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7081 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta
     7141 attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc
     7201 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7261 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7321 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7381 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     7441 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     7501 ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga
     7561 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     7621 tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     7681 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     7741 ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     7801 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     7861 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     7921 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     7981 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8041 aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga
     8101 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8161 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct
     8221 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8281 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     8341 cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     8401 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc
     8461 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg
     8521 atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat
     8581 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     8641 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     8701 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     8761 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     8821 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     8881 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     8941 taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat
     9001 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9061 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9121 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9181 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9241 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9301 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9361 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
     9421 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
     9481 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt
     9541 tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca
     9601 aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg
     9661 cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga
     9721 tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt
     9781 tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta
     9841 atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct
     9901 tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg
     9961 aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca
    10021 cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag
    10081 atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct
    10141 actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt
    10201 gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca
    10261 gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg
    10321 atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata
    10381 ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc
    10441 acgattgggg tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc
    10501 cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga
    10561 atattcggga acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct
    10621 tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata
    10681 tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt
    10741 ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc
    10801 tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat
    10861 atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa
    10921 cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata
    10981 taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat
    11041 tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc
    11101 gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg
    11161 gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg
    11221 atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt
    11281 gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct
    11341 aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg
    11401 tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca
    11461 gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt
    11521 ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac
    11581 agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca
    11641 gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt
    11701 tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact
    11761 gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa
    11821 gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat
    11881 aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa
    11941 cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat
    12001 tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt
    12061 tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt
    12121 tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg
    12181 tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg
    12241 gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc
    12301 tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg
    12361 attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac
    12421 aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc
    12481 tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg
    12541 tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg
    12601 atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt
    12661 gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct
    12721 ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc tatttcttta
    12781 taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc
    12841 atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga
    12901 tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct
    12961 cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg
    13021 gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt
    13081 acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg
    13141 tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac
    13201 gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt
    13261 cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct
    13321 cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct
    13381 tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca
    13441 tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata
    13501 tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg
    13561 tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc
    13621 gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta
    13681 atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt
    13741 ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact
    13801 ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg
    13861 attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct
    13921 ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac
    13981 tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc
    14041 tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt
    14101 tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact gactggagag
    14161 gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat
    14221 gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa
    14281 cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta
    14341 cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa
    14401 actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc
    14461 caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc
    14521 cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt
    14581 ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc
    14641 atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat
    14701 ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa
    14761 gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt
    14821 aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc
    14881 tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca
    14941 ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat
    15001 tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac
    15061 aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac
    15121 tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc
    15181 gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt
    15241 cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta
    15301 attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa
    15361 cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt
    15421 ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa
    15481 ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag
    15541 cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca
    15601 attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat
    15661 ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag
    15721 ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac
    15781 aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat
    15841 aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat
    15901 ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac
    15961 gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc
    16021 cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt
    16081 attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg
    16141 aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt
    16201 tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga
    16261 tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt
    16321 gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac
    16381 cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca
    16441 tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg
    16501 catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt
    16561 gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc
    16621 acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt
    16681 actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac
    16741 aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc
    16801 tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg
    16861 tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg
    16921 gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt
    16981 caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag
    17041 ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa
    17101 ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt
    17161 ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag
    17221 cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac
    17281 agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt
    17341 tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa
    17401 taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc
    17461 attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac
    17521 ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc
    17581 ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata
    17641 caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc
    17701 ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac
    17761 tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa
    17821 tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt
    17881 ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct
    17941 cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt
    18001 catttctcca cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat
    18061 ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata
    18121 taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc
    18181 actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt
    18241 tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac
    18301 gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat
    18361 ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat
    18421 atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg
    18481 gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt
    18541 caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc
    18601 aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga
    18661 atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg
    18721 tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat
    18781 tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg
    18841 gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc
    18901 ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt
    18961 actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca
    19021 gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa
    19081 taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta
    19141 gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat
    19201 cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat
    19261 gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca
    19321 acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag
    19381 tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt
    19441 cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac
    19501 gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat
    19561 tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt
    19621 ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt
    19681 gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg
    19741 gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat
    19801 ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg
    19861 ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat
    19921 ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga
    19981 catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct
    20041 tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt
    20101 ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc
    20161 ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc
    20221 aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag
    20281 gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc
    20341 caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca
    20401 tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc
    20461 actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc
    20521 ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat
    20581 ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc
    20641 ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca
    20701 tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc
    20761 gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca
    20821 acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat
    20881 cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag
    20941 ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac
    21001 ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca
    21061 tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga
    21121 taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac
    21181 ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca
    21241 tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa
    21301 atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata
    21361 acacaagatt gactaataac agaataataa atttattatc cgttacgccc ataatcaacg
    21421 aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag
    21481 ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat
    21541 cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta
    21601 tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa
    21661 tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt
    21721 tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc atgattccta
    21781 gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac
    21841 cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag
    21901 ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag
    21961 ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa
    22021 ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca
    22081 acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga
    22141 gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag
    22201 ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga
    22261 atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata
    22321 acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg
    22381 ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc
    22441 ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct
    22501 gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata
    22561 ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt
    22621 cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca
    22681 tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta
    22741 aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag
    22801 ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc
    22861 ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg
    22921 ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc
    22981 ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt
    23041 tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat
    23101 ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt
    23161 agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat
    23221 caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc
    23281 taaaagctct accattttga tagcggtttc gctttgcatc acacggatct tctttccctt
    23341 caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc
    23401 ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat
    23461 ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga
    23521 aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc
    23581 tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg
    23641 gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt
    23701 cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc
    23761 atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc
    23821 tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt
    23881 ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact
    23941 gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc
    24001 ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt
    24061 ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt
    24121 tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat
    24181 ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg
    24241 cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt
    24301 caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg
    24361 catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca
    24421 tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat
    24481 tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca
    24541 actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag
    24601 gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt
    24661 tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg
    24721 gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag
    24781 gattttttgt catctggtaa tttttataaa taccatatag accgacacct tgtggccaat
    24841 tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac
    24901 cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta
    24961 attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc
    25021 tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa
    25081 caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg
    25141 aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt
    25201 atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca
    25261 attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt
    25321 cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac
    25381 tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta
    25441 attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat
    25501 ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat
    25561 agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc
    25621 cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac
    25681 cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg
    25741 atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat
    25801 ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat
    25861 ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg
    25921 gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg
    25981 caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt
    26041 tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac
    26101 caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg
    26161 cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc
    26221 ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca
    26281 catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca
    26341 taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata
    26401 gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc
    26461 gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt
    26521 tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac
    26581 cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac
    26641 cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc
    26701 caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca
    26761 tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca
    26821 attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt
    26881 ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg
    26941 catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt
    27001 ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa
    27061 tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg
    27121 ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg
    27181 tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg
    27241 gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat
    27301 tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag
    27361 atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca
    27421 ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac
    27481 cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg
    27541 ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt
    27601 tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt
    27661 gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc
    27721 tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta
    27781 cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg
    27841 gtcatggtta tttgttaaat aattcacatg tatatcatac aatatttatt tttctatgta
    27901 aacacttttt tgtgattttt ttcacag
//
LOCUS       DANKAZ010000032        25904 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000032 DANKAZ010000000
VERSION     DANKAZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            339..2399
                     /locus_tag="PWY67_001275"
     CDS             339..2399
                     /locus_tag="PWY67_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL2057571.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2482..3051
                     /locus_tag="PWY67_001276"
     CDS             2482..3051
                     /locus_tag="PWY67_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2057572.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3226..4032)
                     /locus_tag="PWY67_001277"
     CDS             complement(3226..4032)
                     /locus_tag="PWY67_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057573.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4170..5684
                     /locus_tag="PWY67_001278"
     CDS             4170..5684
                     /locus_tag="PWY67_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL2057574.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(6087..7070)
                     /locus_tag="PWY67_001279"
     CDS             complement(6087..7070)
                     /locus_tag="PWY67_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL2057575.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(7075..7722)
                     /locus_tag="PWY67_001280"
     CDS             complement(7075..7722)
                     /locus_tag="PWY67_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2057576.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7807..8439)
                     /locus_tag="PWY67_001281"
     CDS             complement(7807..8439)
                     /locus_tag="PWY67_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2057577.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8455..9006)
                     /gene="coaC"
                     /locus_tag="PWY67_001282"
     CDS             complement(8455..9006)
                     /gene="coaC"
                     /locus_tag="PWY67_001282"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL2057578.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(9003..9740)
                     /locus_tag="PWY67_001283"
     CDS             complement(9003..9740)
                     /locus_tag="PWY67_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL2057579.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9737..10726)
                     /locus_tag="PWY67_001284"
     CDS             complement(9737..10726)
                     /locus_tag="PWY67_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2057580.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10984..11526
                     /locus_tag="PWY67_001285"
     CDS             10984..11526
                     /locus_tag="PWY67_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2057581.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11543..12628
                     /locus_tag="PWY67_001286"
     CDS             11543..12628
                     /locus_tag="PWY67_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2057582.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12640..13446
                     /locus_tag="PWY67_001287"
     CDS             12640..13446
                     /locus_tag="PWY67_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057583.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13447..14274
                     /locus_tag="PWY67_001288"
     CDS             13447..14274
                     /locus_tag="PWY67_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057584.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14271..15344
                     /locus_tag="PWY67_001289"
     CDS             14271..15344
                     /locus_tag="PWY67_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2057585.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15772..18468
                     /locus_tag="PWY67_001290"
     CDS             15772..18468
                     /locus_tag="PWY67_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2057586.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18545..19033
                     /locus_tag="PWY67_001291"
     CDS             18545..19033
                     /locus_tag="PWY67_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2057587.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            19045..19539
                     /locus_tag="PWY67_001292"
     CDS             19045..19539
                     /locus_tag="PWY67_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2057588.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19560..20369
                     /locus_tag="PWY67_001293"
     CDS             19560..20369
                     /locus_tag="PWY67_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2057589.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20366..21187
                     /locus_tag="PWY67_001294"
     CDS             20366..21187
                     /locus_tag="PWY67_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2057590.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21238..23337
                     /locus_tag="PWY67_001295"
     CDS             21238..23337
                     /locus_tag="PWY67_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL2057591.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23334..24452
                     /locus_tag="PWY67_001296"
     CDS             23334..24452
                     /locus_tag="PWY67_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2057592.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24491..25369
                     /locus_tag="PWY67_001297"
     CDS             24491..25369
                     /locus_tag="PWY67_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2057593.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25560..25712
                     /locus_tag="PWY67_001298"
     CDS             25560..25712
                     /locus_tag="PWY67_001298"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2057594.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8212 a         4491 c         5453 g         7748 t
ORIGIN      
        1 aatattttgg aaataccatc atcagagaaa aaaatagaca tttaactgaa aaaatcagct
       61 agtatttatg gatggcagct ttgtctcaca tatttattag ctgatttatg tttttttcaa
      121 agaaaaggta actagatata actagatata actagatata ttacttaaca aaattaaaaa
      181 gtacataagt tattattccg attttcttga aacaataaaa aatcaaaaaa aatactctcc
      241 taatagtcca tcttcttttg attttattta tataatgaaa tcatcttatg aaaattacta
      301 cttcatgttt agacaagagt agggaggact aagaataaat gtgttcaaaa agacgttcaa
      361 aacaactaaa taaacacgct tgtcgtttag ataaggaacg gtcgtgttct gaaaaacact
      421 acgttaagtt tatgatgcgc acgatgctga tcagtttatc tgtaggtgtc tatatgcatg
      481 tacccgttca ggcaacgaca caaaccatca aggtcgtacg tacctctgtt tctgagacca
      541 ctgattcccc tatcactatt actcatactt tcactgatgc aaatggaaaa cagctacttc
      601 ctgcattgac ttcctataat aaagtcagtt atcctccaac gataaaccac tactggttaa
      661 ttcctaacaa aacattatgg acatcggaaa ccggaagcag tgtgccgctt tctatctttt
      721 ccgatatgac tgatcctaca aaaggtgatc agttaaaaca gatcactgat attttgaatc
      781 atgctttgac agatataaag gacggtggaa gcgcacaagt ttcttatgta tatgaaaaag
      841 attattcaca gtttgaaaga aagaaaatcg atgttctggc agggacaaaa gtttcgatat
      901 ctgatctggt agaaaggctg aagaacgcag atggaacaca aggagatatg tccaaagtaa
      961 tttcaaacaa tgggaatatc gtggatacga ctaatacagg aacatctact atttccttgt
     1021 cctattttga tccaattgct ttaaaaacaa tgactgcatc tgctgtgatc catgttcgtg
     1081 ctgatcaaac agctatcaaa ggaaaagatc ttcacttaag aacaggagag agttacgccc
     1141 ctaaagatct tgttgaatat gtaacaaaaa gcgatggaac ttcaggaaat aaagaagaaa
     1201 tcaaaatgaa cgggacagta gatactacaa ctccgggaga atatcccgtt gtattaactt
     1261 atatagactc gaaaacatca aatgtggcag aaacaacagc tgtagtttat gtagaagcat
     1321 ctgctgcttc atcgactgtc atcactcatt ttatagatag tcaaacaaat aaagaacttc
     1381 aagcagaaac acatatgagt agtgtaaatc gtattcctga tttgcgccta cctgctgaat
     1441 tttctgatcg agtgccaaat atagatttat cgacttatga acaaaatggg aaagtcactt
     1501 ctatttctga ttggatgcaa gcaaacggat tagacagaaa aagtcactgg tcggagatcc
     1561 ttgaagtact tcactcagaa ctgatgaata ataggaagca atcaggaggg gaaattacct
     1621 ttacttatgt ttatatacca gacaatcgtt cattagaagg tcatgatctt attgttcatc
     1681 caaacgaaaa aataaacaaa gaagagttga tccaatcagc aacaaattcc ttaggtaaac
     1741 ccgtaccgtt aaaggatgta tcggttacaa tcaatgaagt accattggag gatggttatt
     1801 tgaccaaaca aacaggtgat tttacgatta tgtatagtta tcatgacccg tattcactcc
     1861 aagaactcca tgctcagtca ctattacatg ttttaccatt aaacaaacag atagatcaaa
     1921 aagaacaaaa aagacgagaa gaaagcttgt cagaaaaaaa acaacaaatg gctcatgcag
     1981 tttctccaac agggaaagta aaacaaacaa gaagcctaga gttagcaaga atatctagaa
     2041 aaatctcaga atctaataaa acgataaggc aagaagctca tttggcccca caagtttctg
     2101 gattggaaaa tgaaatttta gagcctaaaa taaaacaaca atcagaaaaa gaatttgttg
     2161 ctaaacagtc ggaagatcag aaggaatcag atgaccagaa ggaaacgaag aaaaaacgct
     2221 tgtcaactat tgattccacc gatgagcatc tgcagccgca agtaccactt cctagtccgg
     2281 gaacacccgc acctggtgga ggtattcctt cttctgcttt aggagatttc atgcgaggta
     2341 tggcaggaac atgggtatac gctaatcgaa atgaatttga cgcagatgac tggttctgat
     2401 ttatatctct atctgttata gtcaaataag tagctgagtc atatgttatt tggcataatg
     2461 gagacgagga gggatagaaa aatgttagat aaaacgttac cttacgcaga aatctggatg
     2521 acaagaccac ttcaacaaaa tgttccaatc acgcctttag cggaaggttt tgaatttcga
     2581 ttttatcaaa aagaagatga atctgcttgg gcggagattg agacttccgt tggggaattt
     2641 caaacgacag atgaagcact tgagtatttt caacaaaact ttgctcctta tcctgaagaa
     2701 cttgaaagaa ggatgttttt tgtagagaca aaaaaaggag aaaaagtagc aacctgtacc
     2761 gcttggcgaa aaaaatcaag agagcaagtg tatccggttt ttcattggtt agcagtgaaa
     2821 ccagattacc aaggaaaagg gctcgcaaaa gcattgactg ccagagtctt gcaagaattt
     2881 cctaccctcc atacggacgg accaatctat cttcacacac agacatggag ccatcaggcg
     2941 attgctcttt ataaaaaaat gggttttact tttattgctg aaaatctaga cggcacacct
     3001 aatccagatt atgaaaaagt tatgcaggtt ttaagtaaat tagataaata acgaaatggt
     3061 gaaataagtc ctcagtattg ggataaaaaa cgaaatagtc attcctcata tcttactttt
     3121 ttgagttaat actatcggat aactaatggc atgccataca ttcaaaaaag aaggggcagt
     3181 gacaaaagta ttgtcactgc cccttctctt gttttttagc atgttttatc gtttgatttt
     3241 tttaggaata aaattcttga tgaacgtaaa tttttggtaa tcgtttcgac tatagaggta
     3301 accaaataca ctgaaaacaa ctgctccaat aaaattaaca aataaatcct tcatagtatc
     3361 catgattccc acgtctaagt aaccattttg gatgattgtt ttatctgttt taccatctgc
     3421 attttgactt tcaatgattg tccgagtgat atctggaaca tgatagacag tgtttccgtc
     3481 tttacttaac tccacactat tgatttgctg gatgatcgta tctttttgca tatcgaattg
     3541 cagtaattta tctgctgaaa attccacaaa ctcccacata acaccaactg tcatcgaaaa
     3601 acagaaggaa acaactgcta gtaataaagg agtcaagttc atggtttttg tatttttatt
     3661 gagcaaataa acaagggaaa agccgacacc cccagctaaa aatccgctga atgcatgtag
     3721 aaaagtatcc cacattgaaa aatagccata aaaatcactt aactctccta aaactgttga
     3781 actaaaaata aaagataaca aaatgatttc tagcatattt ggaatctgta tctttaatat
     3841 cttttccaga aaataaggga tttcaaataa aattaatgtc agtaataaaa gcattgtatt
     3901 tccccagtgt tggttccaga cctgatccac tagcataata acgacgatga tacgtaataa
     3961 aatatagatt ccgtttgtga ttctacctgc agtttttgta ctgttcgtaa aaaaggtttt
     4021 caaatgattc ataaaataac tccttcatgt atatccttaa ttatactaag aagcgtgtgt
     4081 ttaccaaagc tatatgactt aaagaaaaac agagttgata ttgtgaaaaa aatcacttaa
     4141 tatagtataa ggagaaaagg gggatacaaa tgaaaaaatt agttgctggt ttattaacag
     4201 ccgttgcact tcttgctatg agcgcatgcg gcgcagatca gaaaaataca gcgcagacaa
     4261 gttctagtac gaaggaatct gccgaagtat catcaggcgc ttctgaacaa gtatatacag
     4321 atccaaaaga aatgaaagaa tcttatgatg tcgtgattat tggagctggt ggtgcaggga
     4381 tggcagcagc tatcgaagca aaagatgctg ggttgaatcc agtaattttg gaaaagatgc
     4441 ctgttgccgg aggaaacaca tctaaatcat caagcggtat gaatgcttca gaaacaaaat
     4501 ttcaaaaaga agaaggaatc aaagactcga atgatgcctt ctatgaagaa acgctaaaag
     4561 gtggtggagg aacgaatgat aaagaaatgc ttcgatactt tgtcgatcac tctgctgatg
     4621 cggtcgattg gctagatcaa aacggtatca aactggacaa cttaacgatc actggaggga
     4681 tgagtgaaaa gaggacacat cgtccgtctg atggctcggc tattggtgga tacttagtgg
     4741 aggggttatt gaataacgtc catgagcggg aaatccctat ttttgtcaac gcagatgtga
     4801 cagacattaa gaaaaatggg gaaacggtca atgaagtgac tgtacatgtt caaggagaag
     4861 aaccaaaaga ggtcaccgga aaagcaatcg tagttaccac aggcggtttt ggtgccagca
     4921 aagaatttat tgaaaaatat cgtcccgatt tagctgatta tattacgaca aatcaagaag
     4981 gatcaactgg agatggtatc accatgattg aaaaattagg tggacaaacc gttgatatgg
     5041 ataaaataca aattcatcca actgtgcagc aggatgaggg ggtcttgatt ggtgaggttg
     5101 ttcgcggaga aggagcaatc ttggtcgatc aagaggggaa aagatttgtc aatgaaatgg
     5161 atacaaggga taaagtatct gcagcaatta cagcattgcc tgaaaaatca gcttatttga
     5221 tttttgacca aggcgtgcgt gatcgtgcca cagctattga attttatgac caaaaaggat
     5281 atgtcacaac tggaaagaca atggaagaat tagcagaaaa aatcgattta ccaaaagaaa
     5341 cactagcaaa aacagttgat ggatggaata cggatgttgc ggagaaaaaa gatgctcaat
     5401 tcaaccgtgc cacagccatg gaacatgact tatcgtctcc gaattattat gcgatcaaaa
     5461 tcgcaccagg aatccattac acgatgggcg gtgtgaaaat caatacgaac actgaagtat
     5521 tagataaaga aaaccagcca attagggggt tatatgctgc tggtgaagtc acaggtggct
     5581 tacatggaaa caaccggatc ggtggaaatt cagttgcgga gatcattatt tttgggcgac
     5641 aagcgggcat acaagcagct cgatttgctt cggaaaatga atgaaataga acgtaaataa
     5701 aaagaggttg tgaaatcagc gttttgagct aataccgagg gtcagctgat tgaacactgt
     5761 ttagtgaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaagtaaaa
     5821 acagcttctt atgtttttaa cttggttgga cttattaccg cagatgcaag cttttgaaca
     5881 ccgtttattc ggaaaaagag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta
     5941 aacacgtata aacggcggaa gcagaagaaa ttccttcgga aataagctga aattcacaaa
     6001 aatttgaaaa gcaattttcg tgaatttctt cttatttctt ggagttaaac gcttctgtcc
     6061 cagcctcttt tttgatcagc atgattttat tcagttatct tcaaaatgat ttttccgatg
     6121 tttttgtttg cttccatata ttcatgcgct tgtttgactg cgctgaattg gaatgtacgg
     6181 tcaatgattg gccgaatctt gccattttct agaaaaggcc cagctttttg cataaaagtt
     6241 tgtgcgagct ctgctttata ctgatcactt ctaggtgtca ataatgtacc aagcagttgg
     6301 acacgtttcc caatcaatac accaaggtct atctcaggca cgatggtccc acctagcata
     6361 ccaatcaaga cccaacgtcc atcaacggct atagaagcta aattcttttc ccagtaagaa
     6421 gcgccgatga aatccaaaat aacatcgact ccccgacctt cagtgatttt tcgtacctct
     6481 tcttgaaagt cttgtttttt atagttgatc aattcatctg caccaagttc tttacaaaag
     6541 gcgagttttt cttctgatcc tgcagtaacg atgattttcg cgtgagaaag ctgtctagct
     6601 aattggatcg ctgctgttcc cacaccactt gctcctgcat ggatcaagac agtttcgcct
     6661 tcttgtaatt ttccaagcca gtaaagtgtt tgataagcag tcaaaaaaac ttctgagatt
     6721 ccagcagcag aaacatagtc tagcgtatca gggagaagga ttgcacgatc ggcaggcatc
     6781 acagcatatt cggcataacc acctagattg actaatccgt atacttttgt cccaggagta
     6841 aaagtggaga tatcactttt attctcaact acctctccag ctacttcgac tcccaaaata
     6901 gggtagggag gtttcaaaga gactgattct cttttcataa tatctgttcg attgacagct
     6961 gctgtatgga ctttgatcag taattcgcct tgttttggtt taggaacagg cacttcaatt
     7021 tgttctaata catctacaga accaggatgc ttgattgaaa tggcacgcat ggatctattc
     7081 ctcctttgcc cattcttgga atgctgtatt caactctttg ctaccttcag aaagtactag
     7141 agtcgtacga gaaatatccg tttcttccac tagagcggca agtacagcag caactgtaga
     7201 gcgagtgatc gtttttgatg ccttctcatt aggcttagtt aattgaatgg aagtaacttc
     7261 ttcatcgtta gtcaatgtga ctggttgaag aatgacaaaa tctaagttag ttcgtttcag
     7321 ccattcatct gcataatgtt tagtaatata ataatctgtc atggactcag gccattttgc
     7381 tggttcatct gcataaacag cactgaccat cagatagcga gagacatgcg cattttcagc
     7441 tgcgatcact gttttgattg cgccatccaa gtctacttgc agaagattct ttccttgaga
     7501 gccggcagca aaaatcaata catcgatgcc tttgaatgct tcggtcattt tttccacgga
     7561 ccaagttaaa tcaaaagaga catacgaaac attctctttt tttactaatg tttgggaagc
     7621 aacatctctc acccctgcaa aaatttcatg ggaacttgga gctaaatctt ttatcaggtg
     7681 tcggccaatt tgtccgtttg cacctacaac aaaaattttc atttggatca tcctttcgac
     7741 gtcaaatctt tccattcaat agtaacacca aaagagaaaa aaatcagaga tcggctacga
     7801 tgactatcag ttagtaagag aagggtgctt gaagtatctt aacaagacat tgcatactgc
     7861 tgcagtcgca agacctgcga cgattccttc aggtatacca cttgtaaaaa taaccgctag
     7921 aatagctgaa taaacagcat ttaaatttgt tccgatgact ttggcataat cttgctggaa
     7981 taaaaagtag atcatattca tcactaaaat cgtattggtc aatgaaccgg aaagtccagc
     8041 taagaataaa gaaacggaat ctgctttttg tttcattagt ttgcgcagac ctttataaac
     8101 gaaataagga accaccccaa tcaagatgcg aggaataaac gcgatcagca aggctttcca
     8161 gcttccatgt tctgttccga tgactgggat aaacggagaa aagacaaaag acaaaggtgt
     8221 ttggataact gtgctacgga tcatgctgat cagtccaaat gtaccgccaa gaaaagcacc
     8281 tagtcttggt cctaaaacga tcgaagcaat gattacagga atatgcatgg ttgttgcatt
     8341 gattggaccg atagggataa aacctaaagg cgtaacagct aataaaatca aaatagctaa
     8401 aaacatcgca gttaatgtaa agttttttgt atttttcata agtactcctt ttttctaagc
     8461 atttctttca ttaagagtca gcatgacctg ttcgatgatc gtctgaatat cagctaaggc
     8521 accttttcca aaatctccac aagctaaaag agattctctt ggttcaattt cctgatagcc
     8581 gacagatctc aatgtttcca gattacgctg agtgatcggg ttttgataca tattcgtgtt
     8641 catggcaggg gcaatcaatt ttggtgttcc cactggaagc gccattgcga ctgttgaaag
     8701 aagatcgtct gcaatgccgt ttgctagctt gccgataatg tttgctgtag caggcgcgat
     8761 gagaaacaag tcagcttgtt ttgctagttc gatatgattg attacctgcg gatttgattc
     8821 ttgcatgacg tctgtatgga ctgcgcgctt cgacagagat tgcaaggtta atggcgtgat
     8881 aaaatgtgtg ctgcttttag acataatcac atctacctgg gcacctaatt tgacgaattg
     8941 acttgtaaga tcagcgcttt tgtaagcaga aatacttcca gtaacgccta gtaagatctt
     9001 tttcattttg attcctccag tctaattttc tcagtaatca atgcagcaat ctcagattta
     9061 ctttgagctt cttccactgt tccatcttta gataagagat agccgtggtg tgtctcgcca
     9121 tggatttccg tcaaatcatt tgcgaaaata aaatcagttt tgttatttat cagactattt
     9181 tttgcgactg ccagaagaga ttcctttggt acatctacta atagtttgaa accaatgatt
     9241 tttgcttttg gctgccattc acgtagataa gaaatgattt ttggtgtttt cttcaaaacg
     9301 agcacaagat gctcggtgtt agaagagatc tttgaaacag tggtatctgt ctgttcattt
     9361 tccgcaaacc aattgtccag ttcttcttgc gttgaagaag tgggtaggtt ttttgccagc
     9421 tgttcttctg aaaaactaaa agcaggagta aaatcactga ctgccatact gtggataatc
     9481 gcatcgtagt gttctttttt cgttagttct tctaattgta aaaataagtc cagtgcagat
     9541 tcgatcgtat aaatcgttat attggagtgt cttttcggtt tcagtgcttg tttcgtagtc
     9601 acgtaatcga ttgtggtatc cggattggca gctaaataat cagctaatgc ttgccctaat
     9661 cgtcctgtcg aatgatttgt aatagcacgt acttgatcga ttttttcagt cgttcctcca
     9721 gctgtgatta gaatcttcat ttatggtcac ctacatttac tagtgttctt ccttggtgtg
     9781 ttccagcaag tagtttagat gctgtttcag gcaattgatc cagagtaatc tcttggacga
     9841 ccagttcatc tgcaatctgc aaatccgcca tctgttgcca taaagaaaga cgttgatcta
     9901 ttggtacatt gacagaatca attccaatca actggatgtt tcgcagaata aaaggcaaga
     9961 cagtcgcgtt tagtttgatt cccccagcat tgccacataa agctactgca ccgttataag
    10021 agatcaatgg aagaagactg cttagctgct cgccacctac tgtgtcgatg acatagtcga
    10081 tttgctgttt tcctaaagct tttgttgtct ctggcagaaa ttcatcaggt cgtacgactt
    10141 gcaaagcacc tagacttttt agccatgtga cagactcttc ttttctggaa aaggcaatga
    10201 ttgactggaa tcctagttta tgtaacaggg ctatcgctgt actgcctaca ccgccagaag
    10261 caccagtaac gacaactttt ttatcggagg tcataccttg acggattaaa gcgttcacac
    10321 acaacatagc agtaaaccct gctgtaccaa ggatcatcgc ttgacgcagc gtcatatttt
    10381 ttggtagagg aactaaccag tctcctggta ctttttgata ttggctataa ccgcctggat
    10441 gggtgacgcc aagcccaaaa ccagtagcaa gtacaagatc ttcttttgaa aaacgtggat
    10501 tgttactttg gacgacagtt ccggataaat caatccctgg agtcatcgga tatgttcgta
    10561 tcacctgacc agactcgctc attgccaatg catctttata attgacatca gaataggcaa
    10621 tttttaccaa gacttcattt tcagccaact cagtaaatgg cagatcactg attgctgtat
    10681 ggaaaacagg ttcctttatt acctgaaaag atcgaaaagc gttcattcgt gagacactcc
    10741 ttttaaaatt ttcttttatc ataccataaa gcctactctt ttattcttgt tcttccgtaa
    10801 atttagacaa aaattaggtg aaaaataaag aatcaaacaa ctagaattta ttccaaaaga
    10861 taaagaaata ttcagcaaaa aaaattttac aaagggttgc ctttttcaat aaatttcgta
    10921 tactaatacg agtggtagta aacaggaagt ttactattag aaaacttcga ttcggaggaa
    10981 attatggaaa ttggtgagaa gttaagaaat ttgcgcatcc aaaagaattt gacacaagaa
    11041 gaactaggcg aacgcacaga tttgtcaaaa gggtatattt cccagttgga acgagattta
    11101 agttcaccat caatggaaac attcttttct attcttgaag tcttaggtgt gactccagaa
    11161 gaattttttc atcaagaagc agcgaatctg caagtggttt attcaaaaga agatcatacg
    11221 gtttatttcg atgaagaaaa cgggtatgaa ttggaatggc tcgtggctga ttcgaatgaa
    11281 aaagaaatgg agcctgtctt gctgactttt gaaggtgcag gagaatataa gacgtttgaa
    11341 ccttcattat ctgaaacgtt tatttttgta ttagaagggg aattagagtt aagcttagga
    11401 gagacggtct atgtggcaaa agcaggacag tcgatctatt atcaagcgac aaaacaacat
    11461 caattaagga atcatctcaa aaaacgaaca aaagtattga ttgttgcaac gcaatcatat
    11521 ttgtaatata gggggtaaaa acgtggggaa accaatcatt tcatttgatc aagtagtgaa
    11581 acgctatgat gatgaaacag tattaaaaaa agtaagtttt gagatcgaac aaggaaaatt
    11641 ttatacactg cttggtcctt ccggctgtgg taaaacaacg atcctacgta ttatagctgg
    11701 attcaccgaa gctacagaag gggacatcta ttttgaaggg aaacggttga atgatttgcc
    11761 agctaataag cgacaagtaa atacagtctt ccaagattat gcactttttc ctcatatgaa
    11821 tgtttttgat aacgttgctt ttggattgaa gatcaagaaa atgtccaaat cagatatcga
    11881 aaagaaggta aaagatgctt tgcgtatggt ccagcttcct ggatacgaaa aacgggagat
    11941 tagtgaaatg tctggtggtc aaagacagcg tgtagcgatt gcacgagcaa ttgtaaatga
    12001 accgaaagta ttgctattag atgaaccgtt atcggcgctt gatttgaaac tacgtacaga
    12061 tatgcagtat gaactgcggg atctgcaaca acgattagga atcacgttta tttttgttac
    12121 tcatgaccaa gaagaagcgt tggcaatgag tgatgagatt tttgtcatga ataaagggaa
    12181 aatcgtccaa agtggtacac ccgtagatat ttatgatgag ccaatcaatc atttcgttgc
    12241 tgattttgtt ggggagagca atatcgtgga cggtgtaatg atcgaagata atctagttga
    12301 gtttgttggt aagcaatttg aatgtgtcga tggcgggatg cgtctaaatg aaccagttga
    12361 agttgtttta cgcccagagg atttgacgat cacgacacca gataaaggaa aactagtcgt
    12421 aacggtcgat actcagctgt ttcgtggtgt ccattatgaa atcatctgtt atgatgaaca
    12481 gcaaaatgaa tggatggtcc attcaacgaa aaaagctaaa gaaggaagca aagtaggatt
    12541 ggcctttgag ccagaagata tccatgtcat gcgtttcaac gagtctgaag aagaattcga
    12601 tgcacgtctt gacagctacg aagaataggg gggacaagaa tgaaaacaat gcgcaggatc
    12661 tattccgtcc cttatatttt gtggctcggt ttattcgtga tcgcgcctgt attaatgatc
    12721 atttatcaat ctttttttga tatgaatggg caattcacgc tgaataatta tctatcttat
    12781 tttacgtcgg ggacatcgct ttctatgacg atcaattctg tatggtatgc atttttgatc
    12841 acgtttttca cgctgatcat cagttacccg actgcttatt ttttaacaaa actgaagcac
    12901 aagcaattat ggctgatgct gattattttg cctacatggg tgaatctgct gcttaaagct
    12961 tacgcattta ttgggatttt cagtgtgcac ggcagcgtga atcatttctt tgattttatc
    13021 gggatcgggc cgaaacagat attattcaca gacttcagtt ttctgttcgt ggcaacgtat
    13081 atcgagattc catttatgat tatgccaatt ttcaatgcat tggaagagat gaatccttct
    13141 ttgatcagtg ctagtagaga tttaggcgcg aatagctttg agaccttccg acgagtgatt
    13201 tttccgttat cattaaatgg tgtgaaaagt ggtgtccaag cagtatttat tccttctctt
    13261 tctttattca tgctgactcg tctgattggc ggaaatcggg tgatcacttt aggaacggct
    13321 atcgaacagc atttcttggt tacacaaaac tgggggatgg gttctacaat cggtgtgatt
    13381 ttaatcattg ctatgttcat cgtgatgctc ctgacaggcg agaagaaaaa aggaggtcgc
    13441 tcataaatga aaaaatttaa atggtcgtat ctttatttag ggatcgtctt tttattctta
    13501 tatgcaccga tcttctatct gattttttat tccttcaatg aaggaggaac gatgaacagc
    13561 tttacaggat ttacttggga aaattatgaa gctgtttttg aagatacacg tttgatcatt
    13621 atcgtattga atacgttcat gctggctttc ttatctgcat tgatcgccac gatcatcggg
    13681 acatttgggg ctatgggaat ctattacacg aaaaaacgac gtgcacggac agcgttgttg
    13741 agcatgaata acatcttatt agtctctcct gatgttatta tcggtgctag tttcttgatt
    13801 ttctttacac tactaggatt cggattaggt tttcagagtg tattgctttc ccatattgct
    13861 ttcagcattc caatcgtcgt cttgatggtc ttgccaaaat tgcaggaaat gaacgattca
    13921 atggtcgatg cggctagaga ccttggtgct aataatttcc aagtagtcaa aaatatcatt
    13981 ttgccttatc tttctccagg aatcatagct ggatatttta tggcatttac ctattctctt
    14041 gatgattttg cggttacatt tttcgtcaca ggaaatggct ttacgacgct ctcagtagag
    14101 atctattctc gtgcaagaca aggaatcagt ttagaaatca atgcgctaag tgcgttagtt
    14161 ttcttgttct ccatgatatt ggtcattgga tactacttta ttagtaaaga aaaccgtcca
    14221 aaacggctga agaaaaacag aagagtacgc gagggggtgg cacggctgcg atgaaaaaat
    14281 tacaatctct gtttttaggt attctcatca ttattgcggt gctgctgctt ggtgttcgtc
    14341 aacttgaaaa aacaagtggc atgagtggag caaaggtact taccatctat aactggggag
    14401 attatatcga tcctagtttg atcacgaaat ttgaaaaaga atatggatac aaagtgaatt
    14461 atgaaacatt tgattctaat gaagcgatgt tcacaaaaat ccagcaaggt gggacaaact
    14521 acgacatcgc tataccaagt gaatatatga tccaaaaaat gatcaaagaa aaactagtac
    14581 ttccattaga tcattcgaag atcaaaggat taaataatat tgatccacga tttttagact
    14641 tggattttga tccagataat cagtattcga ttccatattt ttggggaact ttagggatcg
    14701 tatataacga taaattcttt gatgcaaatc aaattaagca ttgggatgat ttgtggaagc
    14761 cggaattaaa agacagtttg atgctgattg acggtgcacg agaagttatg gggctttctt
    14821 tgaatagttt aggctattct ttgaatagta agaatatggc acagttgaca gaagcagcaa
    14881 ataaattgaa tcatttaaca ccaaatgtca aagcaatcgt ggcagatgag atcaaaatgt
    14941 atatgatcaa tgaagaagcc tccgtggcag tgacattttc tggcgaagct gccgatatga
    15001 tgtgggaaaa tgaacattta cattatgtga tcccaccaga aggatccaat ctttggtttg
    15061 ataatatcgt tatgccaaaa accgtgaaaa ataaagaagg tgcctatgat ttcatcaact
    15121 ttatgctaga accggaaaat gcggcacaaa atgcagagta tattggttat tctacaccaa
    15181 atgaagaagc aaaagcgtta ttgccaaaag acatttcgtc ggacgaacaa ttctatccat
    15241 ctgatgacac gatctcccat cttgaagtct acaaagatct aggatcgaaa tacctaggca
    15301 tctataatga cctattctta gaattcaaga tgtataggag ataatggcaa aactcagtaa
    15361 gaatggggac ttatcaacgt tctaaagagc taaaaatcgt cctttttcta tctgagtttt
    15421 ccaatcttct accaaaagcg gatgaaacgc cgcttttttt gtgttatttt attttttcta
    15481 ccacgttttt ctaccagaga aaagtggaca aaaccattgt aaaatagggc ccgagacggg
    15541 ctagtaaatc taagattgaa aaagttacag aaggataaag taattggaaa ctatgcaaat
    15601 atacttaacc cttctgtaat ctattaataa aactaatata tgaatccagt tcaatatttg
    15661 tagctatgaa aaaacataaa aatcatatta gataaaaact aaattggtag ataaattatt
    15721 ttgtcagata tctgaggcaa atattcgact atttgtgttt caaagtaaaa attggaatac
    15781 aaatatagaa tagacatatt gcagccgata gtttattatg cttttagaca cattaaactg
    15841 gaggaaaaaa tgaaaaagag cgctaaagat cgagtttttg atgagctgat acgactaaca
    15901 gggaatatgt cttctgtcac tgccgagaaa atttcgaaga atctaggtat ctctcgtcag
    15961 aatgccagcc attatttgac gagattagtg gaagataaaa aggtcgagaa attaccggga
    16021 aagcctgttt tgtggaagcc tttagatgaa tatgcggttg tagataatac ggagcaaata
    16081 aacgaagcct ttcattctgt agttggtcat gacggaagtc tgagagaggt cattcaaaaa
    16141 tgtatagccg ctgtcaaata tccgcctaac ggactcagtg tattaataaa tggagccacg
    16201 ggtgttggaa aaagtttttt agccacaaaa atttttgaat acgctatcca tgagcaaatt
    16261 attgaaaaag atgcaccatt tgctatctta aactgtgccg attatgcgga caatcccgaa
    16321 ctgctttcgg caacactgtt tggatacaag aagggggcct ttacgggggc tgaaaaggat
    16381 accgatggtt tattggcaac ggccaacaac ggctatcttt ttctcgatga ggtccatcgc
    16441 ctatcgaaag aaaatcaaga aaagctcttt ctatttattg atactggaaa ctatcgtcca
    16501 gtcggtgaaa atgtaaattg gcattctgct aaggttcgtt ttattttcgc cacgacggaa
    16561 aaaggtgaaa attatttatt agacaccttt gatcggcgta ttcaaattag tgttatgttg
    16621 ccaacttttg aagatcgccc cattcgagaa cgattagagc tgattcaatt attttttcaa
    16681 aatgaagcgg atgttttaca aaaagatatt attgtttcaa gagaagcctt gtctttaatg
    16741 ttatctcatc cctttagtgg gaatattggt aagctgaaga atatcattaa aatcagttgt
    16801 gcggatgtgt atagtcgaac acaagaagaa ccattaacga ttgggaaaaa tgagatcttg
    16861 attcagttaa atatgctgga atccgaagta gaaagcttgc cgattgctag tttattgata
    16921 aagaaaaatc aaaagataaa aacggatcga atcaaaccgt ttgattatga ttcgattttg
    16981 gaaaaaatta ctcagctgat aaatgaaggt aatttcaatg aaaatttttc ttcgataaag
    17041 aatgaagtac agcatttggt gcatcacatt gataaggatt cttttttatc aatgagtaga
    17101 aaattgatga ttcagatgtt tagaaaggtc tgggtatccg ttttaggaaa aacgtacgga
    17161 ttgacgacga gtggacctgt tgcagaaatt attagcagat cctatgtcat ttccccaagc
    17221 tataagacag acttaaccgg gataaatcgt acattagctg caaatctacc tcggacgact
    17281 tacttatgta atcagttctt ggagcattta ccaccgctct cagaaaagga cgagaacttt
    17341 ctaagactgc ttattgtcgt gactttatct gattatgtag atgaatccat cgagctgaag
    17401 ggcctgttgc ttgctcacgg agatagtacg gctagcagca ttcaagcagt agtgaatcaa
    17461 ttatgcggca attatgtttt tgaagcgttg gatatgccag tagagagtaa tgtgtcagag
    17521 attgtcgaat cgacaaggaa tttcgtgaaa cggcaagtga atacagatag tctagtgctg
    17581 atcgtagata tgggctcttt aaatcaaatt tacacacaga tcaagaacga tttaaaaggg
    17641 gagttacttc ttctgaataa tttgacgact tccattgcac ttgatattgg cttgaagatg
    17701 acgatgaatg cgccatttaa acagattgcg gaggatgcag cagacaagta ttcaatcaat
    17761 gttcaatact ttgaaggctt ttctcaaagt agcaacatta tcatttcctg tatgtcgggt
    17821 ttggggattt cggacaaatt gaaagatgtt tttcgtcatc atttatcgga tgaatccatc
    17881 gaagttttca cgaaggacta cagagaacta cgtgaattaa tcgaccaaaa tgatgaaact
    17941 tattttgaca agacaaaatt ggtcatcacg acttcggatc ttccacgttc cttttcaatt
    18001 cctaatatta atgtttatga catcttagac ggtgatggag cagccaattt ggagcatatt
    18061 ttgagtcctg aaatgaagcc ggcagaattt aaccgcttga ttcaggagct agtaaaattc
    18121 ttttcgttgg aaggaattgc ggaccgtttg aactttctga acccaacgat cgtgatgagt
    18181 gaagtggaaa cagtcatttc taaatatgaa aattactatc atttcacttt aaatggccgt
    18241 gtaaaactaa atctctatat gcatacggca ttgatgatgg aacgtttatt cttggctcgg
    18301 acagaaaaat ctgaagaacc gaattatgat ttgaaaccag atgaagatga attttattat
    18361 gtttctagaa gtatttttca atcaatggag atgaaatata attttaaagt caatgcttat
    18421 gagctttcgc tgctctatga attattagga ccgtatattc aaaaataaag tgtctaaaaa
    18481 agtgtctaat ctgttttttt taggttagac acttttttta atgtgaatta acgtgattaa
    18541 accaatgttt ttaaaagttg gcacaggaat tgcttatata aaagtgaaca gaaagaaagg
    18601 aattaaccat atgaagaaaa ttctaatagc gactcacggt cacttagctt caggctttct
    18661 tagcagtatt caattactga cggggaaggt tcaagagatt acggtgatta atgcttatgt
    18721 ggatgagtgt gattttgaac aggagttgaa tgtatttgta aaccaggtga acgagcagga
    18781 tcagattttt gtttttaccg atttatttgg cggaagtgtc aatcagaaga ttaccaaaac
    18841 ctttttggag aaagagattg ctgtaactat tttgtctggc tttaatctgc caattctgct
    18901 cgaaatagta ttggcaacca aggaattaac aaaggaggat attcaggaac tggtaaataa
    18961 atgccaacag gaattaaaga tgaccattct tgatgaggta catgtagtaa atgaagagga
    19021 agattttttt taggaggaag aaaaatgatt aaagcagtgc gaattgatga acgattaatt
    19081 catggacaag tagcgatgac ttggacaaag agtctaaatt taaccgggtt agtagtagca
    19141 agcgatgagg cggctaccaa cgatattcaa aagatgacat tgaagatggc tgcaccatcg
    19201 aatgtcaaag tcattattaa aacagttgat gacgcgattc atttgttaaa agatccgcgg
    19261 gcgaaggaca tgcgcttgat ggttttagtc cctaatgtca aagatgcggt aaaagttgcg
    19321 aaagctttcc caacggaaat cgaattaatg aacgtgggga atgctgggaa aatgacaccg
    19381 ccatcggaag ataagaaaac cttatcaaag gaagtgatgt taacgacgtc agaaattcaa
    19441 gacttaaaag aattagttga actttatcca gataccgttt ttcagcctac accggcgatg
    19501 gaaaaacgtt cagctatctc tattttgagt gatttttaga aaaaggaagg agaaaaatta
    19561 tgtatgatgc atttgtagtc gctttggccg tttttattgg aattgctgga catgaaatgt
    19621 tcgggatgtc catgctgagt cgtccgattg ttgttgctcc gttagcagga gcgttgatgg
    19681 gggatttgca gacggggttg attgtcgggg cgtcactaga agccatcttt atgggtgtcg
    19741 tcaatatcgg aatctctagt acggcggagc cttctttggc tgctgggctg gctacagcgt
    19801 tcgcaatcaa aatgggagga aacgtggatg cgattattcc cattgtcttt ccactagcag
    19861 tattaggatt acagttaatg aatatgattt tttcttttgt atgtggaccg atggctgcta
    19921 aatttttcaa atttgcaaaa tctggtgatc aaaaaggaat catcaatctg cacttcttta
    19981 tgtggtttgt ccactatgcg ctttatgctc ttattccatt ctttgccgtt ttatttggag
    20041 cagatgttgt caaaagcgtc ttggataata ttccagaggt catcatgaac ggattaacag
    20101 tcgctggaaa tctgctgccg gcagttggga tggcgatgct gttacggatg ctttggagta
    20161 aaaatattgc tgtgtggttt ttctttgggg ccgttgttat ggcgtatctc gagttaccat
    20221 tgattgcaat tgccgcagtt ggaacgatta ttgcaattgt tatagcccaa agagatctac
    20281 aactaaaaaa tatatctgtt catgcagttc aaagcgatgc tgctgtaaat gcaacgcttg
    20341 accaagaaga ggaggatttc tttcaatgaa attagctgat ggaatttcta aagatgagaa
    20401 aaaattggtt cggaagatgt tttggcgctc gatgactatg tatgcttcgg tgaatccggt
    20461 gacgatgggc ggcggtggtt tcgcctattc tatgatgccc ttcattaatc atttctacaa
    20521 aagtgaagaa gatcgagctg cggcgctgga acgtcataca cagtattttt cgacgaccat
    20581 tccttgcgcg tcctttgtca tggggattgc ggcatcgatg gagaaggaaa attctgaaaa
    20641 agctggtttt gacgtggaaa gtatcaacgc gatcaaaacg agtttgatgg gaccattggc
    20701 tggaattggc gattcattgt tctggggagt ttggcgtgtc gttaccgcgg gactagcgat
    20761 caacttagcc aatgccggaa atattttggc accaattatc tttttagtta tgttcaatat
    20821 acctaattac ttgatgcgat actatggcgg cgttttaggc tattcattag gctctaaata
    20881 tattgaaaag ctgtatgcga acgggttaat tgaaattctg acaaaagctg ctagtatctt
    20941 gggtctgatc atggtaggtg caatgacgat cagtatggtc aaattcaata ccgttttgag
    21001 cttttcaatg aagggcgaag aactaatgaa tgtccaagaa atcctcgata gtatttttaa
    21061 aggaattgta ccgttattgg taacattgag ctgtttcaaa ttactagata aaaaagtcgg
    21121 cgtcatcaaa attatttttg gaatggtcgt cttgggaatt ttattgtcat tacttggaat
    21181 cgcatagtag gaagctgcag ctactaggct gcagtttttt tagcaaggag ggaaaagatg
    21241 actattaatg aagtaattta ttcacccaaa cacccatttt ttaatgctga gttaaacgac
    21301 ggaaatgtga ctacttcaaa cgaatacagc tactatcgga atattcctga tgtaaaggga
    21361 ttgaccttcg atgtaagtaa tcaaaagaca ttagcaaacc ttgatgtttg cgggacgatt
    21421 caaacgttga ccttttatcg agaaaattta ctaactgagg aaaaaccagg tgtttgggtc
    21481 aataaacagc tctctcaaac caattcgtta tccttaaagg ttgaagtaaa cgggattgtc
    21541 taccatttgt cagaatcgga tcatcgtatt gaagtcgatg tgattcaaga ctgtttgcct
    21601 agatatattc atcattattc gacgtttaag gtcattgtag tatcctttgc tcccatactg
    21661 gctaataagc gtttaagtat gcttgttcaa caggtttaca ttgtgaatga aacacacgaa
    21721 agccttcaag tttcagtgaa agatgtgcct tcgtatcaag agaagtattc ggatcagcaa
    21781 aatgtgttga tttctcaaaa aggaaataag gacacgatcg cacaacagga agtggcaagc
    21841 ttcgccattg cttttgtgga cccaaatgct tttgaagaag taatgacttt tcaggaatct
    21901 gatatcgaaa aatggctgaa ggaaacattg aactattttg ctcagctatt tgggcagctg
    21961 tcaatgccaa acgatagagt tgtccatctc tatcgtcgtg cactttatca aagcttttct
    22021 agtttcggta tgaatcgaca gggacaggtc gtcggcagca attggggcag ctatcctgct
    22081 actaatcgaa tttggaataa ggatatgtat tacgcatcat tgccattcac catgtttgaa
    22141 ccggagttat gtcaaaaaac ggttctatgg tttgatcaat atggcgttaa gtttcctggt
    22201 tcaaaattta caggaggtat caatcattct ctgagtaatt cgttatccag tgcgttactg
    22261 gctagtcttt attttgaaca tactggggat acaaaattct ttgagcggtg tccgcaggtt
    22321 ctactgaatg cttcaagaat tattgaggac atattagctc aacgtcggtc aggggaacca
    22381 atgctttttc catctgtttg gttatcagac gcctttgcac ttggaaagta tcataccggc
    22441 agcaatcttt gtctttggaa ggcctgtatt ggacttgcag aaatttttga agccctagaa
    22501 caactgagtc ttagcaagcg atacaaaaac attgcctgta aactaaagga atcaatttgt
    22561 aaacaaatga ctatcgatgg tccttttggt gagcaatttt tggagggaat cggtgatgag
    22621 gagaaaacaa cctattcagt gaagcactat caaaaaccga ttcttgagca agggttaatc
    22681 tttttaagtg atgtgattgt ggatggaaaa attaatttat tgatgcatga tggagaagaa
    22741 tcagacacta cgttgatgcc tttttatcaa tttttagata ataaagatca tttatatcag
    22801 aacaccatgg ccttttctgc tagttctttc aatccaacct atagcgagga aatcaaaggc
    22861 atcacttggg gattagaatc cggcgccact tttcctggct ttatcactgt tttaatgagc
    22921 gagctgcaca accctcaagc tttcgtaacc cgattagaag aactaatttg tttggcagat
    22981 ttagacggtt cgtggtggtg gtggccgtat cgcttagagg ctcaacaagg agaagtcgtg
    23041 cgagactttg gctgcggcaa atgcggctgg gcatcgggtc tttttgtcag cctaatgatc
    23101 agtcaatatt tcggattgaa attcaaaaaa ggagtattgc aaattgatcc tgttgataat
    23161 ttaacgtttt cttggaagaa tttgaagttc ggtcaagcct tcatttcgat tgaatgtacg
    23221 caatccgagt tgagtatttc aaatttgtca gaaaagccat tgaaaataag tgatacgaaa
    23281 aaaattcttc acgtagaaaa gggaaaaacg attcacattc aaaggagaaa aagatgagta
    23341 tactaaaaaa tattacccaa tttaaaaaaa tccagagtcg agcaatcact cccgaaaatc
    23401 caacaggcga gaaaggtaaa gctgcgatgt tgccaagcga gctaggacca agccgcaaag
    23461 gtcgtggcgc aattccgctg acttctggtg aagaaactgt gattgcggat attactggaa
    23521 cgggaataat tcgtcatatg tggatgacga ttcgtgatcg aacagaattt ggcagttttg
    23581 ttttacgtga tttaatctta agaatttatt gggatgggtc ggatagccct gctgttgaaa
    23641 gtcctttagg ggatttcttt tgtaatgggt ttggagagcg ttgcgaggtg aactcgttgc
    23701 cgatagtggt taatcctaca gggggcttta attcctattt cgaaatgcct ttccaaacat
    23761 ccgctaaaat tacgatcgag aatttacatc cgaaggatat gtcctcattt ttttatacga
    23821 taaattacgc tttggtggac gaattggaag aaaatagcct atattttcac gcaaattggc
    23881 atcgggaacg cattaccaaa ttacaggagg attataagtt aattgatcga ttaaagggtc
    23941 aaggctatta cgtgggtact taccttgcgc ttacctgcct agaacgttat tggtggggcg
    24001 aaggcgagtt taaattctac attgatgggg atagtgagta tccgaccgta acctcaacag
    24061 gttcagaaga ttattttggc ggggcgtggg cctttcatga gtacgatgag ttcggacgtc
    24121 cacatgcgaa aaactattca acagcctttt tagggtatcc ttaccaatca aatagagatg
    24181 ccacaagaga tcgttttgaa acgggaaaat tgaatgccgt tcatgccttt ggtgacgacg
    24241 caatgccacg acatggtctt tatcgttggc atcttcaaga ccccatctgt tttaccgaag
    24301 atttatcggt cactttacaa caaatcggca acgatgatgt tcgcttattt gagcggcaag
    24361 acgatgttgc tagtgtcgct tactggtatt caacagaagc ttcaggaaat actaaacgtc
    24421 atttcactga tcgagaacgc ttgccgcgtt aatgaagtgt ttttcacata ttcgaaggga
    24481 ggaacctatg atgatatatg gggcaattga agctggaggg actaaatttg tctgtgcagt
    24541 cggtactgag gaattaacga ttttggaaag agtcagtttt cccacaacaa ctccagttga
    24601 aacaatgaag caggtcattg atttttttaa accttttaag gaacaattgg caggcatcgg
    24661 ggtcggctgc tttggtccaa ttgatattca atgcaaatca acgagtttcg gtcatatcac
    24721 atcaacacca aaacttgctt ggcaaaattt caattttgta ggaacgctaa agcaatattt
    24781 caatattccg attgaatgga caacagatgt caatgcggct tgctatggtg aatatgttgc
    24841 tggttctggc aaagggttag caggtgttgc atacttcact gtgggaacag gtatcggagg
    24901 tggtgccttg ttcaatggga ctttcgtgga agggttcagt catccggaaa tggggcatat
    24961 gttgatcaag cgacatccaa aggacaattt ttcaggaaac tgtcccttcc accaagattg
    25021 tttagagggg atggcagcag gaccagcgat tgaaaaacgg ttgggtgtaa aaggccagaa
    25081 tttattggct gacgattcgt tttggcagat tgaagccttt taccttgccc aatgtgcata
    25141 caatacaaca ctgatgtttt cgccagatag aattattttt ggcggtggtg taatgaaaca
    25201 agaacacatg aagaaaaaag ttcaggataa atttgttgag cttataaatg gatatgttga
    25261 aataccacca attgatagct acattattac gcccgaatta ggtgataatg ccggaataat
    25321 cggcggatta gcattggcca gaaaagcggt aagaaataag caaccataat taatgaatgt
    25381 ttcattgata ctcatgtcaa aagtatttaa ccgagtaatc aaatgatcaa atgaagttgg
    25441 ataaagcgaa aatgaattca aataaaaagg ctctaagtca aatcgtgttg ttttcattaa
    25501 atcactgaaa ggaaatgctt ctgcgtttcc ttttttcttt ttgttgtgcg ctgtatacct
    25561 tgaggatatt cactggtggc gtacacttaa atagtaatat attgtgttac tttatgtctg
    25621 tggtaatagt tatagttgct taccaatgta tgttcatttt tcgaaatgtt gacttcataa
    25681 tccttggctt aatgtttgaa tttttttggt gatagatttt ttcagccgta taaaataaat
    25741 cgtaattcta ttatagagag ttgtgattat aaaaagatag caagaaaaaa aaagaaagtt
    25801 tctatacatt ttaaatattt tattcctatt tctattgcta aaaaagtaga aattctatat
    25861 ttacctttaa ctctcgcttc tcttgcaaat gtagttagca tttt
//
LOCUS       DANKAZ010000033        25361 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000033 DANKAZ010000000
VERSION     DANKAZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..411
                     /locus_tag="PWY67_001299"
     CDS             <1..411
                     /locus_tag="PWY67_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2057595.1"
                     /translation="NGSYTLRDMHMMPEIKRNVLEKQLKKTIEKNNSIIDSNLSNAIN
                     NIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPIAYLAALLNTLLRYDSLINDSE
                     KNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            472..1617
                     /locus_tag="PWY67_001300"
     CDS             472..1617
                     /locus_tag="PWY67_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2057596.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(1698..2003)
                     /locus_tag="PWY67_001301"
     CDS             complement(1698..2003)
                     /locus_tag="PWY67_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL2057597.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(2028..2459)
                     /gene="ruvX"
                     /locus_tag="PWY67_001302"
     CDS             complement(2028..2459)
                     /gene="ruvX"
                     /locus_tag="PWY67_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL2057598.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(2456..2722)
                     /locus_tag="PWY67_001303"
     CDS             complement(2456..2722)
                     /locus_tag="PWY67_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL2057599.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(2977..3702)
                     /locus_tag="PWY67_001304"
     CDS             complement(2977..3702)
                     /locus_tag="PWY67_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL2057600.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(4063..4133)
                     /locus_tag="PWY67_001305"
     tRNA            complement(4063..4133)
                     /locus_tag="PWY67_001305"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4099..4101),aa:Gly,seq:ccc)
     gene            4250..5002
                     /locus_tag="PWY67_001306"
     CDS             4250..5002
                     /locus_tag="PWY67_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2057601.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            5018..5497
                     /locus_tag="PWY67_001307"
     CDS             5018..5497
                     /locus_tag="PWY67_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2057602.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(5748..7328)
                     /locus_tag="PWY67_001308"
     CDS             complement(5748..7328)
                     /locus_tag="PWY67_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL2057603.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(7321..8064)
                     /locus_tag="PWY67_001309"
     CDS             complement(7321..8064)
                     /locus_tag="PWY67_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2057604.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(8240..9016)
                     /locus_tag="PWY67_001310"
     CDS             complement(8240..9016)
                     /locus_tag="PWY67_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2057605.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(9264..10100)
                     /locus_tag="PWY67_001311"
     CDS             complement(9264..10100)
                     /locus_tag="PWY67_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL2057606.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(10111..11040)
                     /locus_tag="PWY67_001312"
     CDS             complement(10111..11040)
                     /locus_tag="PWY67_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL2057607.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            11232..11585
                     /locus_tag="PWY67_001313"
     CDS             11232..11585
                     /locus_tag="PWY67_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2057608.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(11659..12315)
                     /locus_tag="PWY67_001314"
     CDS             complement(11659..12315)
                     /locus_tag="PWY67_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2057609.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            12541..14475
                     /locus_tag="PWY67_001315"
     CDS             12541..14475
                     /locus_tag="PWY67_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2057610.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(14561..15784)
                     /locus_tag="PWY67_001316"
     CDS             complement(14561..15784)
                     /locus_tag="PWY67_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2057611.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(15778..17676)
                     /locus_tag="PWY67_001317"
     CDS             complement(15778..17676)
                     /locus_tag="PWY67_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL2057612.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(17670..18788)
                     /locus_tag="PWY67_001318"
     CDS             complement(17670..18788)
                     /locus_tag="PWY67_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2057613.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(18871..19704)
                     /locus_tag="PWY67_001319"
     CDS             complement(18871..19704)
                     /locus_tag="PWY67_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2057614.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(19697..20614)
                     /locus_tag="PWY67_001320"
     CDS             complement(19697..20614)
                     /locus_tag="PWY67_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2057615.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(20841..21965)
                     /locus_tag="PWY67_001321"
     CDS             complement(20841..21965)
                     /locus_tag="PWY67_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2057616.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            22148..23056
                     /locus_tag="PWY67_001322"
     CDS             22148..23056
                     /locus_tag="PWY67_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2057617.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            23197..24810
                     /locus_tag="PWY67_001323"
     CDS             23197..24810
                     /locus_tag="PWY67_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2057618.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8022 a         4931 c         4241 g         8167 t
ORIGIN      
        1 aacggatctt atacattaag agatatgcat atgatgcccg aaattaagcg aaatgtgctt
       61 gaaaagcaat tgaaaaaaac tattgaaaaa aataactcta taattgattc taatctttct
      121 aatgctatca ataacatcga agaagataac ctgactgtct cacagaagat ttatttaaca
      181 aacgcaataa aatatcttaa aacaggatct cgaattgaat ttcctgacgg gagtacccca
      241 attgcttatc tcgcagctct tctaaatacg cttttacgat atgactcatt aattaatgat
      301 tcagaaaaaa atacagatga gaaaaaagag tatcaagaat tttatgaaaa tgatcttcta
      361 aaagagttca cagctcttgt tactgagcta aataaacact attcaaaata ataataaact
      421 gcctcctcca accgccccca cggcttgcta cactatggag ggaaaactat tatggcaaca
      481 ttcaagcaat atgaaactaa aaaaggtaaa ttttggctct atgaagccta tcttggaatg
      541 aacaaaatga ctggcaaacc tgataaggtt cgtagacgag gttacaaaac aaaaaaagaa
      601 gcccagcttg ccttgtcacg cttgcaagtg gactacgata aaaacggtat taaaaaagcg
      661 aacaacgaaa cgtttaaaga agtttatgac ttgtggctag aaacatataa aaccactgtc
      721 cgagaagcca ccttcatgaa aactgaaatc aagtttagaa agtggattct tccaaaatat
      781 ggaaatcttc gtgtgaatga agtcacggta aaaaaagccc aacaaattgt aaatagctgg
      841 gctgaaacta ctgaccaata caaggtactt cattcaactt ctaagcgtat ttttagctac
      901 gctatcaatc ttgggattat tgagactaat ccactagaca acgtattaat gcctaaaaga
      961 accaccacta agcataaaga aaaggtcaaa gtttacagta aagaacaact gatcaccttg
     1021 tttgattacc taaacagcaa acctgaaact tatcgaaacg actttgataa aacgttacta
     1081 cgctttctct tttatagcgg ggttaggatc agtgaagccc ttgcattgaa ttggtcagac
     1141 attgattttc aagaaaatac ggtcaccatc aataaaacac taagccaatc caaaaatggc
     1201 tataaaatca gtgatcctaa aactgattgt agtgctgggg ttcttccact agatgaaaga
     1261 acaatttcta tattaaaaaa atggcagatt aatcagcgga aatatatgct gacacttggt
     1321 gtaactgatc cgactatgat tttttgtggt atctataaag aactaatcac acaccacgct
     1381 atttatgcaa gattgattac tatttgtgaa aaagctatgg ttccatttct cggtgtgcat
     1441 gtagcaagac acactcatgc ttcaatgctc ttggacgctg gagctactat ggtagaagtt
     1501 caaactagat tacggcatac aaaaattagt cagacgatcg attgttacgg tcacttggca
     1561 aaagaaacga aagaaaagac tgttgaaaaa ttagtacaac acttaaatat tgactagatg
     1621 gcatacaata tggcatacag taaaaaataa aaaaaggaga aaccctatca tatcaaggtt
     1681 tctcctagct ttatagtcta ttcttcttct tccgccatga atgtattgaa tacttcttca
     1741 atcatgtccc attctgcgtc tgtttcgatt tgttctaact cgccttctgt tccatcttcg
     1801 ttttcgatgt atgcataagc ttgtaattct acatcttcat cttctgaaac accagcagga
     1861 tataaaagaa catagttacg tccaaattct tcttgtccgt caactgttag taggatttca
     1921 tataatgttt catttccttg gtcatccacc aatgtgatgt gttcgtgtcc ttcatggtcg
     1981 tgggaatggt tatgttctgt cataagatta cctcttttct tttattatta aaataattgc
     2041 ttacctgagc cgtctaaata attttgtaag atcatcacag ctgctaattt atcgatgact
     2101 ttttttcgtt tggctcttga agtatttgcc tgctctacta gcatacgttc tgcctgaaca
     2161 gtggttaatc gttcatcttg ataatcaact ggcaaggaga atagttcttt gatcttttct
     2221 ccgtatgaca tggaagcttc tgcccttggt ccgatggaat tgttcatatt tttaggtaat
     2281 ccaactacaa accggtctac ttcatattgt gctactaatt ccttcaaccg atcaaagcca
     2341 aactcttctt ctgcttcatt gatccgaatg atctctactc cttgagctgt ccagccaaag
     2401 gggtcactga tagcaacacc tactgtctta gaaccgacat ccaagcccat tactctcata
     2461 aattcactcc atgatttgag aggtagtcct tgacaattac ttccatgatc tcatcgcgtt
     2521 cgtgacggcg aatcaaattt cgagcatcac gataacgagg aatgtaggcc gggtctccag
     2581 aaagcaagta gccgacaatc tgattgattg gattgtagcc tttttcttcc aaagcccgat
     2641 aaacaatcgc taatgtttcg ctcacttctt ttttgcgact atcgtcaaaa ccaaaacgta
     2701 cggtttcatc tgtaaaaccc atgattctac acctctttcc cgcaaggtaa ttccagctta
     2761 ccttctacaa ccttatttta ctcgaagaaa gggaaaacta caaacaaata ttccaaactt
     2821 ccgaataaat taagaaacac tatttgtatg tttttttcct tttttctgta gctttatcaa
     2881 aagtactctt ttcataaagt atacgctata tttctttgaa tgtatgtttt tacacaattt
     2941 tttcataata ttcataaaaa attcgaatag ctgctactaa ttttttgttt ttgttgcttg
     3001 tcctgtctca taattgatcg tgtagccatc ttgaacattg tagataaaga cattgaattc
     3061 aatttttttg tcttcaatgc tttctgcttc caattgcact cccctggcta ccagttcatt
     3121 cccctcaaaa tgaggagtga cacggtagcg tacatgatga tttgtttctt tgatatatgc
     3181 cgcaatatca ttttcgtgag caagcatgta tggagcattc aacgaacgtg tccctgtcat
     3241 caagttttta atgttgttat tttgaccagt cagctggaaa ccaattaaat ggctgcgatt
     3301 atataaccat tgcccgtcac ttaatttctt ttgtttccaa cctgtcgggt caatatacaa
     3361 aggctctctc ttttcagtag ggaacaaatc ttttccaagc atagcattgg cttgtcctac
     3421 tctgttcaat ttatcaatat ttgaatagct ttcccatgtt cctttggaaa ggctaagatc
     3481 ctcttttgta aattccggct gattcccatt gatttctatt tcttgatgtt ttccatcata
     3541 ttctggcaca tccgctactt gagaagcttg tggtgtggaa tcaaaaatag ttgtaatctg
     3601 ctgaaagata tctgaatctt tttgtgtggt ggaaacaccg aatgcccctg caatcacagc
     3661 agcgacgatc cccacgatcg ccttggttgt ttttttgctc atttctttac tcctttacta
     3721 acagctccct aaatcttaac atatgttccc ctataaataa atactagaaa gaaatagaaa
     3781 cagcaaaaaa taaaacctgt tttaagatgg cttaaaaata caaaaaagag acggattcct
     3841 cgtctctaat cagtcaccac tctatctgta aggtatttat tattattcaa aaattttctt
     3901 catactcaag acaatacact gatttcacaa ccgcttggta aacggtgcgc aaaagcttgc
     3961 atctgcggta ataagtccat ccaagttaaa aacataagaa gttgtttttg cttggctgtt
     4021 cttattactt gttgcagagc agctttttca caacctattt taagcgggtg acgggaatcg
     4081 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcaatacaa
     4141 aaacagtata cgcctctttt attccttcct caagtttttt tattctttac gcttctggta
     4201 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     4261 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     4321 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     4381 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     4441 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     4501 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     4561 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     4621 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     4681 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     4741 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     4801 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     4861 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     4921 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     4981 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     5041 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     5101 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     5161 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     5221 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     5281 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     5341 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     5401 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     5461 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     5521 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     5581 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     5641 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     5701 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     5761 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     5821 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     5881 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     5941 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     6001 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     6061 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     6121 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     6181 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     6241 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     6301 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     6361 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     6421 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     6481 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     6541 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     6601 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     6661 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     6721 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
     6781 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
     6841 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
     6901 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
     6961 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
     7021 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
     7081 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
     7141 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
     7201 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
     7261 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
     7321 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
     7381 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
     7441 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
     7501 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
     7561 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
     7621 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
     7681 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
     7741 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
     7801 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
     7861 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
     7921 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
     7981 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
     8041 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
     8101 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
     8161 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
     8221 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
     8281 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
     8341 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
     8401 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
     8461 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
     8521 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
     8581 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
     8641 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
     8701 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
     8761 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
     8821 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
     8881 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
     8941 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
     9001 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
     9061 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
     9121 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
     9181 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
     9241 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
     9301 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
     9361 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
     9421 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
     9481 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
     9541 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
     9601 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
     9661 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
     9721 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
     9781 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
     9841 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
     9901 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
     9961 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    10021 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    10081 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    10141 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    10201 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    10261 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    10321 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    10381 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    10441 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    10501 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    10561 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    10621 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    10681 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    10741 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    10801 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    10861 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    10921 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    10981 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    11041 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    11101 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    11161 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    11221 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    11281 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    11341 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    11401 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    11461 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    11521 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    11581 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    11641 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    11701 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    11761 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    11821 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    11881 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    11941 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    12001 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    12061 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    12121 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    12181 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    12241 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    12301 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    12361 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    12421 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    12481 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    12541 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    12601 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    12661 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    12721 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    12781 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    12841 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    12901 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    12961 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    13021 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    13081 caacaccctg acattttgat tctagatgaa ccaacgaacc atttagatat cgagacactt
    13141 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    13201 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    13261 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    13321 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    13381 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    13441 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    13501 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    13561 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    13621 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    13681 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    13741 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    13801 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    13861 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    13921 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    13981 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    14041 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    14101 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    14161 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    14221 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    14281 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    14341 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    14401 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    14461 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    14521 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    14581 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    14641 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    14701 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    14761 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    14821 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    14881 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    14941 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    15001 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    15061 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    15121 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    15181 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    15241 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    15301 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    15361 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    15421 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    15481 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    15541 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    15601 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    15661 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    15721 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    15781 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    15841 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    15901 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    15961 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    16021 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    16081 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    16141 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    16201 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    16261 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    16321 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    16381 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    16441 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    16501 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    16561 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    16621 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    16681 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    16741 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    16801 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    16861 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    16921 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    16981 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    17041 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    17101 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    17161 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    17221 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    17281 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    17341 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    17401 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    17461 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    17521 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    17581 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    17641 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    17701 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    17761 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    17821 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    17881 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    17941 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    18001 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    18061 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    18121 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    18181 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    18241 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    18301 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    18361 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    18421 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    18481 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    18541 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    18601 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    18661 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    18721 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    18781 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    18841 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    18901 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    18961 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    19021 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    19081 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    19141 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    19201 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    19261 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    19321 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    19381 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    19441 actggctgta gctaaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    19501 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    19561 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    19621 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    19681 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    19741 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    19801 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    19861 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    19921 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    19981 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    20041 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    20101 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    20161 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    20221 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    20281 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    20341 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    20401 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    20461 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    20521 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    20581 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    20641 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    20701 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    20761 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    20821 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    20881 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    20941 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    21001 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    21061 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    21121 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    21181 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    21241 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    21301 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    21361 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    21421 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    21481 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    21541 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    21601 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    21661 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    21721 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    21781 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    21841 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    21901 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    21961 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    22021 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    22081 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    22141 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    22201 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    22261 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    22321 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    22381 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    22441 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    22501 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    22561 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    22621 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    22681 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    22741 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    22801 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    22861 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    22921 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    22981 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    23041 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    23101 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    23161 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    23221 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    23281 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    23341 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    23401 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    23461 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    23521 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    23581 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    23641 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    23701 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    23761 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    23821 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    23881 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    23941 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    24001 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    24061 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    24121 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    24181 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    24241 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    24301 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    24361 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    24421 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    24481 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    24541 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    24601 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    24661 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    24721 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    24781 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    24841 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    24901 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    24961 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    25021 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    25081 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    25141 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    25201 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    25261 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    25321 gtgtattaaa taatttaatt tttttgttcc tatttattta a
//
LOCUS       DANKAZ010000034        24141 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000034 DANKAZ010000000
VERSION     DANKAZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            189..758
                     /locus_tag="PWY67_001324"
     CDS             189..758
                     /locus_tag="PWY67_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2057619.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(936..1376)
                     /locus_tag="PWY67_001325"
     CDS             complement(936..1376)
                     /locus_tag="PWY67_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL2057620.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1534..2298
                     /gene="map"
                     /locus_tag="PWY67_001326"
     CDS             1534..2298
                     /gene="map"
                     /locus_tag="PWY67_001326"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL2057621.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2330..3253
                     /locus_tag="PWY67_001327"
     CDS             2330..3253
                     /locus_tag="PWY67_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL2057622.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            3329..4468
                     /locus_tag="PWY67_001328"
     CDS             3329..4468
                     /locus_tag="PWY67_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL2057623.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            4461..5261
                     /locus_tag="PWY67_001329"
     CDS             4461..5261
                     /locus_tag="PWY67_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2057624.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            5261..6088
                     /locus_tag="PWY67_001330"
     CDS             5261..6088
                     /locus_tag="PWY67_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2057625.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            6066..6800
                     /locus_tag="PWY67_001331"
     CDS             6066..6800
                     /locus_tag="PWY67_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2057626.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            6900..7766
                     /gene="rfbA"
                     /locus_tag="PWY67_001332"
     CDS             6900..7766
                     /gene="rfbA"
                     /locus_tag="PWY67_001332"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL2057627.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            7780..8352
                     /gene="rfbC"
                     /locus_tag="PWY67_001333"
     CDS             7780..8352
                     /gene="rfbC"
                     /locus_tag="PWY67_001333"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL2057628.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            8374..9402
                     /gene="rfbB"
                     /locus_tag="PWY67_001334"
     CDS             8374..9402
                     /gene="rfbB"
                     /locus_tag="PWY67_001334"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL2057629.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            9500..10351
                     /gene="rfbD"
                     /locus_tag="PWY67_001335"
     CDS             9500..10351
                     /gene="rfbD"
                     /locus_tag="PWY67_001335"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL2057630.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            10386..12419
                     /locus_tag="PWY67_001336"
     CDS             10386..12419
                     /locus_tag="PWY67_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057631.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            12463..13743
                     /locus_tag="PWY67_001337"
     CDS             12463..13743
                     /locus_tag="PWY67_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL2057632.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            13953..14759
                     /locus_tag="PWY67_001338"
     CDS             13953..14759
                     /locus_tag="PWY67_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2057633.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            14771..15991
                     /locus_tag="PWY67_001339"
     CDS             14771..15991
                     /locus_tag="PWY67_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2057634.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            15981..17567
                     /locus_tag="PWY67_001340"
     CDS             15981..17567
                     /locus_tag="PWY67_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2057635.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            17606..19744
                     /locus_tag="PWY67_001341"
     CDS             17606..19744
                     /locus_tag="PWY67_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2057636.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(20112..21095)
                     /locus_tag="PWY67_001342"
     CDS             complement(20112..21095)
                     /locus_tag="PWY67_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL2057637.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            21473..22864
                     /locus_tag="PWY67_001343"
     CDS             21473..22864
                     /locus_tag="PWY67_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2057638.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            22880..23920
                     /locus_tag="PWY67_001344"
     CDS             22880..23920
                     /locus_tag="PWY67_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2057639.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            23939..>24141
                     /locus_tag="PWY67_001345"
     CDS             23939..>24141
                     /locus_tag="PWY67_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL2057640.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKK"
BASE COUNT         7730 a         4014 c         4952 g         7445 t
ORIGIN      
        1 aaacaatgag gctttaaata aaaaaaggag gttatttttt taaggttaag tttcatgata
       61 tatgttacta cttgctattt cagcttgtga agaaaatcaa aaactaattt tgcatatcaa
      121 aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt tataaaaaaa ttaaggagat
      181 taaaaaatat ggcaagaaaa aagacgatta ctagagaaca gattttggca gctgcttatg
      241 aagtagctgc caccgatgga tttacacgat ttactgcgag aaacatcgcg aacaaaatga
      301 attgttccac gcaaccgatt tatcttgagt ttaaaaatat ggatgacttg agacaagctc
      361 tttttgagca gatctatgat catttacaat atgaagtgtt cactgttgag catacaggga
      421 atacgattat cgatcttgct tttaattata tccattttgc aagaagagag aagaagttgt
      481 atcgttcact gtacttagaa gaatatggtg gaggaaaaga gatgcagaat ttctcttata
      541 cttactttgt gaatacagtg aaacgcgatc cagcttatgt gaatctttca gaagagcaga
      601 tcgaatcttt gtataatggg acatggatca tagctactgg gatcgcagct ttgatgtctt
      661 caagcatcat ccatccaact gatgaacaga ttgaaaagat gatccaagac agtatagatg
      721 ctatcttgaa attcaaagaa ccaatcgaaa tcgattgaat atactcaaaa gttccacaaa
      781 tcagtaggta acaagagatg tgtcaaaata aggaaaagtt cgtttttctt acagcgtatg
      841 catttttaac tcccgaagaa ctcttccatg aatgcaatga ataaaagagc catacagatt
      901 gaataaatct ggatggctct tttattgcaa gtatattaaa tttttgcgac tagttcttta
      961 gcgaaagctt ctaaattttc gatatcttct tcttcagcat tcagatccac tttaacattt
     1021 tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat cgaaatcatc caccgatttg
     1081 cagaattcat cgtaaaaagt atcaccagat ccgcagacac cgtaaatttt tcccaataga
     1141 tccaattctt gcaagtcttc ataaaagtct acgatctcat caggcagatc gccatcatcg
     1201 tatgtataag tagctactac gcaaatatct gcctcctcga attcatctgc atctacttgg
     1261 gtacattcgt tgatttcgac ttcgatatcc agattttcaa atgcttctgc aacaatatcg
     1321 gcgatttctt cagtgtttcc tgtcatgcta gcatatacga tttttgctaa agccatgatg
     1381 tgcctcctcg tttataatta tttaacaatt atgaattata ccaaaacatc attgaaaaga
     1441 aaataaaacc taaagacacc tggggaatgt catttggcta aatgtatgcc gctatgttaa
     1501 aatgagaggg atttgtaaaa aggagacgaa aatatgatta ctttaaaatc agcacgtgaa
     1561 attgaaatga tggcagaatc tggagcttta ttagcagatg tacataaaaa tctgcgtgat
     1621 tttataaaac caggaatcac aagttgggat attgaggtat ttgtcagaaa ctatatcgag
     1681 agtcatggcg gaattgctgc gcaaattggc tttgaagggt atgaatatgc gacttgtatc
     1741 agtataaatg atgaaatttg tcatggattc cctcgtaaaa agcctttgaa aaacggtgat
     1801 ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca tttctgattc ttgttgggcc
     1861 tatgtagttg gagaatctac acctgagatc gaccgattga tggaagtaac gaagaaagct
     1921 ttgtacatcg gtattgagca agcacaagta gggaatcgga ttggtgacat cggtcatgct
     1981 atccaaactt atgtagaagg cgaaaatcta gcgatcgttc gtgattttat tggtcatggc
     2041 gttggtccta cgattcatga agaaccagca gttcctcatt acggagaagc tggaaaaggg
     2101 ttgcggttga aagaaggcat ggttatcacg atcgaaccta tggttaatac cggaacttgg
     2161 aaaatgaaaa tggatcctaa cggctggact gcgtatacaa gagacggcgg actttcttgc
     2221 caatatgaac atacattggc tattactaaa gatggtccaa gaattttgac atcacaagga
     2281 gaagaaggca cttattgatc tatccagtca ataggtacgg gagaggtaca tgaaagtttg
     2341 gaacaaaata aaacaaaatg aaaatctgat gcgctttatt gagacgactc aaaagcgcat
     2401 cactgattca gaaatgagta cgacctctgt cgtcgttgct tattatttac tcttgtcttt
     2461 atttccattg attattgctt ttggtaatat tttaccgttt ctccacatcg atcaagagac
     2521 tgtactgacc tatttacgag aagtgattcc tgagaccgtt taccagttta tcggtcctgc
     2581 aatcaaagac cttttgacac aaagttcagg aggcttgtta tccgtttctg cattagcggc
     2641 tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt gcgatgaata aagcgtatgg
     2701 cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc tcactaattg tcattgtctt
     2761 gttcatgatt gctattagtg gggtaactat cgttcttggg ttgggacagc tgattttgga
     2821 tgctattcag ccaatcgtac aaatacccgt gaactttatc gatcagttcc aaactttgaa
     2881 gtggccaatc acattggtag ctttatttgt catcatgttc ttgatttatt taattgtccc
     2941 taatgctcag ttgaagttga agacgatcat tccgggcacg atcttcgcta ctgtcggctg
     3001 gatgctgtta tcacaagtgt ttagtatcta tgctaggtat tttgcaacga ggatcagcgg
     3061 ttatcagatc ataggaagtt ttattgtact catgctttgg cttaattttg ctgctacgat
     3121 catcattttg ggcggaatca tcaatgcagt tgttcaagaa tacgtgacgg gtcatcaaat
     3181 caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa ttgaaaacta aattttctca
     3241 taaaaaaaag tagaaacaag agttcttatt gtaaatttat atcagtaatt tataataatt
     3301 atgactctta attaaagtag gtgcccatat gcttgtttct tttagtatgg ttataagatt
     3361 ttttctgaca attttattat cactgatatt aacacctgta ttcaaaatca tttcagtaca
     3421 aacaggtatg gtcgacaaac caaatgaaag aagaataaat aaagttccaa tgccttcagc
     3481 gggcggactg cctatcttta tttcatttgt tatatcgact ttattcttat tccgtaaaat
     3541 tatccctcaa tcgtatattg taccgatctt gattgcttct ttagtaatca tagtcacagg
     3601 agtgctcgat gataaatatg agctgacacc taaacaaaaa agtataggga tcttgattgc
     3661 ttctcttatt atttattttg tagcagatat acgtattgat tccttcacgc ttccttttat
     3721 tggatatatc caactaggct ggcttagttt tcccgtaacg attttttgga tcttcggtat
     3781 cacgaatgcg gtcaatctga ttgacggttt ggatgggctg gcggcaggga tttcattgat
     3841 cggtttaatt acgataggaa tcattggata ttttttcctc catgcttcta cagtgtatat
     3901 tccagttgtt attttctgtc ttgtagcaag tattatcggt ttttttcctt acaattttta
     3961 tccggcaaag atttatttag gcgatacagg agctctattc ctagggttta tgatggccgt
     4021 cctttcacta caagggctga agaacgtgac ttttgtttca tcgatttctt tgctgatcgt
     4081 catgggagtg ccggtaacag acacgttttt tgctatcatt aggagaaaag caaaccgtgt
     4141 ttctttttct acagcagata aaaaacatct tcatcaccga ctaatcagtt taggctttac
     4201 tcataaaggt gctgtcctga cgatttatgc gatggctttg atgttttctt tcacagctat
     4261 ggtcatgaat tatactggaa gaatcggaac gatcgtattg attattgcca tgttgtttgc
     4321 tgcaatttta ttatttgaat tgattggtct gatcaatgag aaacatcagt ttattttaaa
     4381 aacattgcga tttttaggga ataaagagta ccgtacgcaa gtaaaacaga aatatttgaa
     4441 gaaatggaac cgtcatatag atgaataaca aattaaacca gccgatcctt attagtattg
     4501 tgacggcaaa cagtgataaa atttttcgga cactagataa ttttatttca actgtaaaag
     4561 atgaaacagc agtaaaaatc cgaatttttg acaacaattc acttcccgag tacaaaaaaa
     4621 gactaacaga gtataggaaa tatccctaca tcgatatcta ttttcatgat gaaaattccg
     4681 gatttggtta tggacataat cacaatctga tagctagcga ttctcgctat ggcattattt
     4741 gtaacccaga tattttagtg gatgaagaga cagttacagc attggtcttg ttattgaaag
     4801 aacatccgga atgtgcgatg ttagcaccta agattcttaa tgaagatgga acaactcagc
     4861 acctcatccg tgagcagctg gctgtttttg attacgtttt gcgctttgtg cctttcaaat
     4921 gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga atgccgagga ttgcctactg
     4981 atgctaatag ttatgtacgg atgatctcag gtagttttat ggtagctgat attgataaat
     5041 ttaaagagat aaaggggttt gatgagcgtt tcttcatgta ctttgaagac aacgacttgt
     5101 gtttgagttt tgaaaaagca ggttacaagt tattgtacac acccttttat gcagttatcc
     5161 atttttacgg gaagggtgcg catcgaaatg caaaactgtt tcgcgtgttt ttgacttcta
     5221 tgatgaaatt tttcaataag tgggggtggc gttttttctg atgaaaccag aaatattagt
     5281 agtcgttgtg ctttatcagc aactattcag tcaatctcca gcttatggac cattaagcaa
     5341 agcacttagc gaaaagcaaa ttcagcttat tatttacgat aatagtcctc aaaggcagca
     5401 aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat gatccgcaaa atcctggttt
     5461 agcggtggct tataattttg cgttgtctca agcagatgaa ggaacacgtt gtctcgtcac
     5521 tctggatcag gatacgaggc tagtcgacga ttattttgaa ctattgcgta aagtaacctt
     5581 tacagatgaa tgtgttgctg cagttccgat gattttttct gggagccgac aagtttctcc
     5641 cctatattca gatcgttata tcaatcgtac agctcaagca gtagaagttg aaggcccaac
     5701 agctgaacga atcatggcaa tcaattcagg tgttgcctgg tctgtcaaat ttctaaaaaa
     5761 gattggcggg tttaatcctg ctttttcttt agattttttg gatcattggc tgttttggaa
     5821 agtggatcag ttggaaaaaa ctgtggagat ccttcctgta cgaatggaac atgatctttc
     5881 ggttttagac tatgaaaaag tttcaagtaa tcgataccgt tctattttac aagcagaaaa
     5941 tcgattttat catgaatatg atcaaaggca tttatccagt caccgacgac aattattgtt
     6001 aagaacagct aaacagttct tgaccgtcaa aaaccggcat atctggcgga tgactttgcg
     6061 ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc ggtttgtata gctaccttta
     6121 atggaggcag atatttaaga gagcagttag atagtatttt atctcagctt tcagcagaag
     6181 atgaagtagt catttctgat gatgggtcag tagatgatac atgggatata ttaactgaat
     6241 acgcagaaaa agataagcga atccagcttt tttcggggcc tcgtcaagga ttgatcgcta
     6301 attttggtta tgcgatccag cagacgaaag gagatctaat ctttttggca gaccaagatg
     6361 atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta ttttgagcag aatccggatc
     6421 agctagtagt agtttctgat ttaatcattg tggacagcga gctgcaagag atccatgatt
     6481 cgtatttttc gtttcgaaat tccaaagaag gatggcttaa taatgtgata agaagtcact
     6541 atattggtgc tggtatggct ttccggtctt ctttaaaacc cgatattttg ccgatcccgt
     6601 cagaagtgcc gatgcatgat atgtggattg gtttgctggg aggaaagaag actgggttcc
     6661 taagagaacc gttaactctg taccgaagac atgatttaaa cgcgagtgag attgaaacac
     6721 attctaaatt acggcaaaaa atttcttggc gtgttcatct gattacggcg ttgatcaaac
     6781 gaaaatggtt taaacgttga taaaagatgt ttaaatcctt aatatttcca tgacaaatat
     6841 aacaattgat gagataataa caaagtgaaa tttgaaatca aaaaggagag cagatatcca
     6901 tgaagggaat cattcttgct gggggcagcg gaacacgtct gtacccttta acaaaggcaa
     6961 catcgaaaca attgatgccg atttatgaca aaccaatgat ttattatcca atgtctactc
     7021 tgatgttggc gggaatcaaa gaaattctga ttatttcaac gccacaagat acaccacgtt
     7081 tcaaggaatt atttggaaac ggaaatgatt taggactgca gatcgaatat gcagtacaag
     7141 aaagtccaga tggattggca caagcgttta tcattggaga agaatttatt ggagatgata
     7201 gtgtctgctt ggtattaggg gacaatattt actatggtgg cggtctgtct aaaatgttgc
     7261 aacgagcagc ttcaaaagat acaggcgcga cagtatttgg ctatcatgtc aatgatccag
     7321 aacgctttgg tgtagtagaa tttgatgaga atatgcaagc tttgtcgatt gaagaaaagc
     7381 ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta tttttatgac aatgaggtaa
     7441 tttctatcgc taaaggtatc aaaccttcag aacgtggaga actagagatt acggatgtca
     7501 ataaagcata tttggataaa ggaaaactat ccgtagaact tatgggacgc ggatttgctt
     7561 ggcttgatac aggaacacat gaatcattgc ttgaagcatc tacctttatc gaaacaattg
     7621 aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat tgcttaccgt atgggctaca
     7681 tcgataagga acaattgctg gcccttgcac agccattgaa aaaaaatcaa tacggtcaat
     7741 atttattgaa tttggccgct gaataaggag tccttacaga tgaaagtaac agatacaaaa
     7801 ttaacagatg tcaaaatcgt agaaatggac gtctttggcg atcatcgcgg tttcttcaca
     7861 gaaagttatt caaaagcaaa atttgcagaa catggattga aatatgactt tgttcaagac
     7921 aaccattcac tatcagctga agctggtgtc cttcgggggc ttcatttcca aaaaggagaa
     7981 gctgcacaga caaaattgat tcgggtagtc actggagcag ttttagatgt agtagtggat
     8041 atccgcaaag gtagcccgac ttatggtcaa tgggacggat ttatcttatc tgaacataat
     8101 catcgacagt tgcttgtacc taaaggatat gcccatggat tcgttacttt gacaccaaat
     8161 gtgaacttcc tttataagtg tgataattac tataatgcag aagcagatgg gggcatcgca
     8221 tttgatgatc cagatttagc gattgattgg ccaattgatc catcaagagc gattatgtcg
     8281 gaaaaagatc accgtcatcc gacattaaaa gaatttgaag cagaaaatcc atttgtttac
     8341 ggagaaatct aagaattaag gagaaatttt cttatgaaaa acatcattgt taccggaggt
     8401 gccggattca tcggctctaa tttcgttcat tacgtggtaa acaatcatcc agaagtgcat
     8461 gtcactgttt tggataaact gacttatgca ggaaacaaag agaacttggc cggacttcct
     8521 agcgatcgcg tagaattggt cgtaggagat gttgcagatg cagaattagt ggaccgtcta
     8581 gtaaaagaag cggacgcagt tgttcattac gcggcagaat cccataacga taattcatta
     8641 aaagatcctt tcccatttgt acaaacgaat atcattggta cgtatacact tttagaagct
     8701 tgccgcaagt atgatgtacg ctatcatcac gtatctactg acgaggttta cggcgatctt
     8761 ccattacgtg aagacctgcc aggacatggg gaaggcgaag gagaaaaatt cactgcggaa
     8821 acaccatata atccttcaag tccatattct tctacaaaag ctggttcaga tctgttagta
     8881 aaagcatggg tgcgttcttt tggcttacgt gcaaccattt caaactgttc aaacaactat
     8941 ggtccttatc agcatattga aaaatttatt cctcgccaag taacgagtat cttaagcggt
     9001 attcgtccaa aattatatgg cgaaggaaag aatgttcgtg actggatcca tacgaatgat
     9061 cattcttcag cagtgtggct gattttgacg aaaggcagaa ttggcgaaac ctatctgatc
     9121 ggtgcagacg gtgaagaaga taataagacc gtgatggaat tgatcttaga aatgatgggc
     9181 caaccaaaag acgcatacga ccatgtaaac gaccgcgcag ggcacgattt gcgttatgca
     9241 atcgattcga caaaattgcg agaagagcta ggttggcagc ctgagtttac gaacttccgt
     9301 gatgggttgg ctgacacgat caaatggtac gaagaacatg aagactggtg gaaaaaagaa
     9361 aaagaggccg ttgaagcagc atatgccaaa aatggacaat aatacagagg aaatttcact
     9421 gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg tctctttttt aaaataaaaa
     9481 tcaaggagcg gataaccata tgtttttatt aactggagga aatggacaat taggtacaga
     9541 attgcgccat ttattagatg aaaaaaatct gccatatgtt tcaacagatg caaaagactt
     9601 ggatatcaca gatgctgaga aaacaatggc ttatattaca gaattaaaac ccgatgttat
     9661 tttccattgc gcagcttaca cagcagtaga taaagcagaa gaagaagcaa aagaattaga
     9721 tgagaaaatc aatgtcgatg gcacaagaaa tgtagcactt gcagcaaaag ctgtgaatgc
     9781 acaattcgtc tatatcagta cagactatgt tttcgatgga aaaaacaaaa atgtagaata
     9841 cgaagtagac gaccaaacga atccactaaa tgaatatggt cgtacgaaat tattaggcga
     9901 aaaagcagta gaagagattt tagaagacta ttatatcata cgaacttctt gggttttcgg
     9961 gatctatgga cacaatttcg tcttcacgat gcaacgccta gcggaaaccc gcgacaaact
    10021 gactgtagta aatgaccagt ttgggcgacc aacttggaca cgtactttgg cagaatttat
    10081 ggtatatgtc atcgaagaaa aagcaccatt tggtatttat catttgtcga acgaaaatag
    10141 ctgttcttgg tatgagttcg caaaagaaat attgaaagat acagatgtag aagttgcacc
    10201 tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg cagtattcag tcatgagcct
    10261 gaaaaagaca gaagcgttag gttttgtcat accaacttgg caagaagcac ttgcgcaaat
    10321 gctggaagct gccaaaaaaa tgaataagta atttcctttt tatcaaatag aatttggagc
    10381 atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa gcgaatcagg
    10441 cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt ggcgttttag
    10501 tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt tgggataaat
    10561 atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag aatcgggaag
    10621 tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct gaagaggact
    10681 tttcgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt gcgtataatt
    10741 ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc tttttcctcg
    10801 gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg gtcttgcttg
    10861 gattcgaagt actgatgata ttaacgaaga ggaaaaaagc tctatcgcta atcggtgcat
    10921 ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg ggcgatttga
    10981 ttttctttac tttgggactg atgattgctt tttatcatta tttctacaat catgataaaa
    11041 aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg tttattttag
    11101 tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat tttgttggcc
    11161 tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg ttgattggag
    11221 gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca aaagacgcat
    11281 tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga ggaaagtgga
    11341 cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa gatatcaact
    11401 attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta ttcctagcaa
    11461 gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga cgtattttga
    11521 tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa tggctagcag
    11581 aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt agttttgcgg
    11641 cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt attcctattt
    11701 ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg attacgcatt
    11761 cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg ttgggaacag
    11821 tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct tttgtcgcct
    11881 tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga gcgatttttg
    11941 aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt aaagtttggt
    12001 tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc ttcaatacag
    12061 tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa gaatacgaga
    12121 agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat acaaagttca
    12181 ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca gaaaaattag
    12241 ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta tatgaagcgc
    12301 agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa gtagaatatc
    12361 cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc gtacagtaat
    12421 aggctagctg agaataatcc tgaaatatta gtagaaagga gagtgaagat aattcaagca
    12481 atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt gctgacgcta
    12541 gtcttttttg tttggacagc aggggtaggt tttccagcaa ctttgtttat atataacaat
    12601 agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt ttataaagca
    12661 aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt cttttcttac
    12721 tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt ggttatttta
    12781 ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat ttatctgatt
    12841 gtctttacgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat ccaagtacca
    12901 gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat caatacgaat
    12961 acaatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc cagttctttt
    13021 gaacgatggt atgttcgaat catcggtggg atagcagtcg ttgcagcctt tttaggtatt
    13081 tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat attggatctt
    13141 ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcctacac ggttacgatt
    13201 cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa tttgtttacc
    13261 gatcgagaag atatttggca taaattctat cagacgctag gagaaaaatc tgaacaagtt
    13321 ttactaggaa tgaaaacatt tatcttccag cgaggaaacc aatttttagg aaaccacaat
    13381 agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc tgctttgctg
    13441 cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg gcagatgact
    13501 tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac attgacttca
    13561 tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg atatgatgaa
    13621 ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat gacttatgat
    13681 gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca acgctatcat
    13741 tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt tggtttattg
    13801 caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta tcatagaaaa
    13861 ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg cacttgactg
    13921 taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat ttatttatga
    13981 aggacattta tcagaacaga aaattattgc tacagttttc gatcaatgac ttcaaatcaa
    14041 gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta tttactgtat
    14101 tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat gcaaaatacc
    14161 catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca gatgcatttg
    14221 catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc gtattcaata
    14281 ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccattta ttcttcattc
    14341 tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg tctttacagc
    14401 tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg ctgacggcat
    14461 cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg cagttgatca
    14521 tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt gtaagcatat
    14581 tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt ttaagtgaag
    14641 cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg ttagtaatgt
    14701 tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat gtgctgtaag
    14761 gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat cttataatat
    14821 gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga aatcctatca
    14881 tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg agatgatcgg
    14941 tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta cgggagtact
    15001 gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc tagaactagg
    15061 atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg gaatggttct
    15121 aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat ttgctgacat
    15181 cggagaccat atttatcaac ctgtaaaaac ttattcaagc gggatgttcg ttcgtctagc
    15241 ttttgctgta gcaatcaatg tgaatcccga tattttgatt gtggatgaag cattggcagt
    15301 aggcgatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca agaactcggg
    15361 aaagacgatt ttattcgttt ctcatgatat cagttcgatt cgtcgttttt gtgatcgttc
    15421 ctattggttg aaaaacggag aagtcgtcga atatggcgat acgatggaag taacgacgaa
    15481 ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg gatcggaata aaaaccaagt
    15541 agaacggttc gccgctccag atatcgtcga cgtgacgaaa gcggaattgt tggataaaga
    15601 caaaaatccg gtggatatgc tggaacaagg agaaaaacta tacgttaaag taacttatga
    15661 agtaaaagat gatacaatca aaaatccggt tttaggaatt gcgctacgaa cagtcgacaa
    15721 tttttatgta tgcggactta atactctact agatggtatc aagattcctt ggaaaaaagg
    15781 agagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg aatattattt
    15841 tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatataaga caaagtacat
    15901 gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag accataaatg
    15961 ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat gaacaaagca
    16021 gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag tttggtcccg
    16081 gaaacggccg attaacgaaa catttaatcg gagcaaaaca atgtgaagtg agcattgttg
    16141 agttagataa ggaattgttc gattttgtca gcgaatttgc tcaagatgga ttttatggtg
    16201 atatcgaaag ttttgagtgg gcaaactatt atgctggaca gacatttgat tacgtcttgt
    16261 ttgcggatgt tttagaacat ttggtagatc ctggaaaaac attgaagaaa gtcagagaat
    16321 ttttgaatga agaaggagag atcctgatta cattccctaa cttggcgcat aattctgtca
    16381 tgatcgattt gttcaataac caattgccgt gggcttctta tggtctccta gatgaaaccc
    16441 ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagcg ggattattca
    16501 ttaatattga agattatttg tacttagctg taggtgatac agaattaaaa tcaacttatg
    16561 aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg accatttggt gaagtctacc
    16621 aatacttttt ctcattgatg aaacatccag tagcacaaag ttccattgca gagccacaaa
    16681 attctaatta tgtaaaagtg ttagaagtaa cccagcaaac gaaacaagat gaaacaatcc
    16741 aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc cccgtatcag
    16801 aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc tagctttatc gaattcagcg
    16861 cagaagctgc cggagaaaaa ctaacattta tcgattcgaa tgctgtagtc aaaacagtaa
    16921 atgattgtta tttgtttgat gggaaagaat taccagagtt cgttctaacg gatatttctg
    16981 ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca ccaacaatga
    17041 aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac aaagcagctt tctgagaaaa
    17101 atgacgaatt agaaagagaa aatcatcatc ttcttgagga aaatacaaga ctagaccata
    17161 cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa tggacaataa
    17221 aacatagact aggacgacgt aagaaagaaa cttcgaaaaa aatccaagaa aaagaacttt
    17281 ctatatgtat tgacgagaaa atctgggatg cagaaacaaa aatattaaaa atcatcggat
    17341 ggggaatcgc taattcggat cgccagccct tgtcctataa gttatcagct gaccaatcgc
    17401 catttttcga agcaattcct gtttcacgag aagaagtcaa tcaagcagaa cagctcgcaa
    17461 aaggaacaga agcgggattt gagttacgaa tcctttgcga gcaagaacgt tcattcttag
    17521 tggaagcagt agctcaaaat ggccaatcat ggatcattga aatgtaaata atgattaact
    17581 tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat ttatcgatca
    17641 aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa ttgatgaagt
    17701 gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg aagcaacaca
    17761 tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc aaagcggttt
    17821 caaggtacgt ttatcaggga aaatgagagg aaaagccatc ttagacttcc aaacagcgag
    17881 acatcaaaat ggcatagctg tgaaattaaa cggtaaatac ccttatgatg acggcattga
    17941 atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg caaggactca
    18001 tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg aagccaggat cgatcgacta
    18061 tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa gagcaaagga aagaagcaca
    18121 aggatttgcg taccgtccat tgatttccat acttatgcct gtctataacg ttgaaatcaa
    18181 atggctcgaa aaatgcattg attcggtact ggatcaaacg tatgatcatt gggaactatg
    18241 tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctggaat cctatcaagc
    18301 aaaagatgat agaatcaaag tcgttttccg aaaagaaaat ggacacatca gtctggcaac
    18361 taattctgca ttggaaatag cagaaggaga atttatagca ctcttagata atgatgatga
    18421 attaccgcca tttgcattat atgaagtggc aaaagtgcta aacgtacatc cagaactgga
    18481 tgtgatctac agtgacgaag ataaaatcga tgcagatggc aatcgctttg atccgcattt
    18541 caaagcagac tggtcgcctg atacattgat gggaaataat tatatctctc acttaggcgt
    18601 atatcgaaca agcattgtta aagaacttgg cggtttccgt aaaggctatg aaggttctca
    18661 agattatgat ttggtccttc gtgtgacaga acaaatccct gcagatcata tttaccacat
    18721 tgaccgagtc ttgtatcatt ggcggacgat cccaggatca actgcaagta atggcgaagc
    18781 caaaagttat atctatgatt ccggagtgaa agcattgaca gatgccctgt ctagaagaaa
    18841 catcaaagga agcgtacgtc ctggcagaat ttctggattt tatgagattg cttatgacgt
    18901 cttgcaagag gatttggtca gtgtgatcat tccaacgaag aatggctatg aagatctaaa
    18961 aacttgtgtg gattcgatca tcgaaaaaac gtcttattca aattatgaaa tcattattgc
    19021 ggataacgga agtacagatc caaaaatgca agaattattt gcagaataca agcatcagct
    19081 gaaagaccgc tttatcgtag aattgattga cattccattc aattattctc gaattaacaa
    19141 tttagcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg atacagaagt
    19201 gattgagcca gattggatga cggtgatggt ttcttatgca cagtttgatc gaatcggctg
    19261 tgtaggagca aaactgtttt atccagatga tacaacgcag catgcaggag tattattagg
    19321 aatcggtggt gttgcagggc atgcattgaa taactatgat cgaacacatt gtggttattt
    19381 cggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta tgatggtcaa
    19441 ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca ttagaagtag cattcaacga
    19501 tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg cacatcaagc
    19561 agaattgtac catttcgaat ctaaatcaag aggttatgaa gatactccag aaaaacaaag
    19621 acgttttgct ggtgaaatca aaaaaatgca agataaatgg ccagcatata ttgcgcatga
    19681 tccattttac aatgataatt taacaaaaga cggaatcggc gacttttcgt tgagaccaga
    19741 ttaaactata ataaaaagct gttatgttgt gattcgctcg cacataacgg ctttttatag
    19801 ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa ataagtcgag gttgggacag
    19861 aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg
    19921 aatttcggct tatttaagaa gaagttgctt ctgctcccac cgtttattcg tttttaaaga
    19981 atgagacaaa agtgtttctt acttttgtcc cacgctctta ttcatgctca gagcgccaat
    20041 agatacaaaa tcatagccaa agtttcacga accctagctg tcaaagaatc gatattttat
    20101 tttaatatag gctattttaa aataaatggt ttaaggtatt cctctacaaa agccactcta
    20161 tcaattatca tgggtttaaa ataagaagtg atggagataa ccagattgtt cttagcgtca
    20221 atataaagaa catttccact attaccaatt gctgaatata tttcgttctc agaatctaat
    20281 acccaccata aatagccata tctcatattt tgtaagtctt ctaaagcact ggttctaggt
    20341 ttgaacatct cgtttatcca catgggtggt acgatttgtt ttcctttata catcccttta
    20401 tttaggcata atacaccaat ttttgccaga tcacttgccg ataaacacaa accatatcct
    20461 gccgtcccga tatttagtgg atcacaaaac cagacattgg ttttaggaga cttatccatt
    20521 gtaaactgct tatgttcttc agctgtttca gcataataat tgacatggtt tggtatccct
    20581 ataggatcaa atagatactt attggcaaaa tcaacagttg ttagcttact tgtctgatac
    20641 aacaagccag ttaatatgtg taaacagaca gtttgatagt tgaattcgcc ggttatacct
    20701 tttcttcctc ccaataaatc tagtgatgtt tttgtccaat cttcacttga acagactttt
    20761 gtccatggat cgcctttaca tttgtagggt gctttcattg ttagcaagtg cttgattgta
    20821 atagcgtaaa tagttttctc cccgcgtttc actttatact ctggaaaata atctaatact
    20881 ttatcatcga tacttttgat taatccttgc tctaaagcaa tgccaatcag caaagataca
    20941 atacttttcg ttgctgacat gatatgacaa gtatcgtctt ttttataacc attccattca
    21001 tctgtataaa cttctacccc atccttatat gcgacaattt gacatatatt tggttgtcga
    21061 aaaccaataa aatcatgcaa ctcttgttta ttcataagat attacctcac tttcttctat
    21121 aagcataatg tcattaaaaa tcgatttcaa ttttttcgat tcttcacgta atacaggtat
    21181 tcttatggac tagacagcgc tttgggaaga tggattctct gaaaaatata aaggttcact
    21241 ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct ttaatttacc aacattagtg
    21301 aaaatcgatt catctggaga tttgtaattt agagtccttg tcttgattag aggtgtataa
    21361 agctttttat tagacatttt taaattccat tacaatttgc ttataaacta ggtttttaaa
    21421 ggtgactatg gtaagataat agagatatta ttttgtgatt aggagtgtat atatgaataa
    21481 aaatggagaa tggagtgctg cacgacggat attcattata ttaatggatg ttattgtata
    21541 caatgcagca atatatagta gtttcttact gaagtttcgt ggagagatcc cattacgaaa
    21601 tttagaaact ttccaacact cagcaattat tatttcagtg atttttattg cgttgaacat
    21661 tttgttgggt gcatatgttt tttataatcg aatgatcagt gacatcgtgt ttgtcacagt
    21721 catcggtcag gtattgatga cactagggat catggtagtg acttttgcgg gccgctggtt
    21781 tgcttttcca cgtcttgtta tcttactttc atttgttctc ggtacgcttt tcttaagtat
    21841 ctatcgtatt ctcgtatata aactttattt acgcgtaagt cgagataaaa aggtcgtcat
    21901 tattgggtta gaaaaggatg tcgcaccagc aatccaaaat tttggttcaa agaaaaacaa
    21961 caaacataaa gtcgaagcag tcattcttga taactattat gcaaacatca aaaatatgat
    22021 taaagacatc gatattgtct atcttgcttc tcatatcgaa gaaactgaaa aaatcaaaat
    22081 ctatcaatta gttactaaaa atgaaaaaaa attgtttttg aatacaacat ttgaaaactt
    22141 gacattgatc aatccaaata ttatgaattt cgaagatgaa agtattattg aagcatctgg
    22201 atttagaatc caacctgaat atgaactatt caaacgtttg tttgatatca tcgtttcact
    22261 tgttttatta gttattgctt caccatttat gttattcaca gcaattttag tcaaagtgac
    22321 atcaccggga ccaatcatct ataaacaagt gcggatcaca aaaaatcaaa aggaattttc
    22381 aatctataaa ttccgctcga tgaccgccac agccgaagca aaatctggac cagttttagc
    22441 taagagcaat gatgcacgag taacgcctgt aggtaaattt attcgagctg tccgctttga
    22501 tgaattacca caaatcttca atgtgctgaa aggcgatatg tcgatcgtag gaccacgtcc
    22561 tgaacgttct ttcttcgtcg atcagttcaa tgcagaaaac ccttattact acttacggca
    22621 taatgttcgt gctggtatca ctgggtatgc acaagtctat ggaaaatatg tctctgatta
    22681 caacagtaaa ttacgttttg atttacttta tatcaaaaaa tattcattga tgcttgattt
    22741 aaagatcttg ctgcaaacca tcaagatcct atttgataaa atgtcctctc aaggacttga
    22801 agaagacgaa aatgtgatct cattggcaga aattgctttt ccgaatgaaa aaatctttcg
    22861 ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc ataatcgtac cagtatacaa
    22921 tgcagaagca actattggtc gaacgattga tattctaaaa gaattggatt atgaagagta
    22981 cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca caggaaatca ttcaagaact
    23041 gattgaaaat gatgaacgtt ttgaattgat tactacgacc aatcaagggc ccggattagc
    23101 aagaaacgaa ggaataagac agtcaaaagg agaatatctc ttattcttcg atgcagatga
    23161 cacgccagta aaaacgatct tgtcagatta tgatcatttg ctagaaaaga gtccaaaagc
    23221 ggatcttgtt gttagttcgt ttgtttttcg tacaatggac gagggaagaa tcgtttccga
    23281 aaaagaaaac ttagttacag aatgtcacta tctaactcac gaagagttta taaaagacat
    23341 gtatcagtta atgaatgaac agttgatgta tgttgtatgg aacaaatgct atcgtagcga
    23401 tattctgaaa aataaccaaa ttctatttaa aacttataat agttgtgagg atcgaatatt
    23461 taacttacat tattatgaat actgtcatca agtgatcatg aacccaaaag tagaatatat
    23521 ttatgaattt gaaggtggga aggggatcac taatcaatac cgacctaaca agttcgaaac
    23581 atttaaagaa ttctattcat tagcaaatac tgtaacagac gatattaata aagacggtat
    23641 ggcggcgctt cttttaaaag ggacaacttc agttattttt tctatttata gtacctctgc
    23701 aagaacttca aaagagaaga aagacgaagc aaagcagata ttgagtgatc cagtgatccg
    23761 agaagccaag caaattgcta agacggattc ttcagcaaaa aaaataacaa agttgcttta
    23821 caatatgcca gctccactat ttttatctgc tgtaaaagtt ggaagcttcg ttgaagtgaa
    23881 aatgccaggg ttaatggctg ctttgaagcg agtatattag ttaatggagg aaaatataat
    23941 gaaaagaatt ttgcattttc agggacgaat gggtttaggt ggagcagagt ctttcatgat
    24001 gaatttatat cgtaaaattg atcgtacaaa ctatcaattt gattttttga tttatgaaga
    24061 ttatgcagat gttcaagact atcattcaga gattgaaaga ttaggaggcc gtatttttgt
    24121 tgtacctaat cctaagaaaa a
//
LOCUS       DANKAZ010000035        23863 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000035 DANKAZ010000000
VERSION     DANKAZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(87..821)
                     /locus_tag="PWY67_001346"
     CDS             complement(87..821)
                     /locus_tag="PWY67_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2057641.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1148..1558
                     /locus_tag="PWY67_001347"
     CDS             1148..1558
                     /locus_tag="PWY67_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL2057642.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1551..2252
                     /locus_tag="PWY67_001348"
     CDS             1551..2252
                     /locus_tag="PWY67_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL2057643.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2252..3838
                     /locus_tag="PWY67_001349"
     CDS             2252..3838
                     /locus_tag="PWY67_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2057644.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3857..4078
                     /locus_tag="PWY67_001350"
     CDS             3857..4078
                     /locus_tag="PWY67_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL2057645.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4075..4836
                     /locus_tag="PWY67_001351"
     CDS             4075..4836
                     /locus_tag="PWY67_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL2057646.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5216..5725
                     /locus_tag="PWY67_001352"
     CDS             5216..5725
                     /locus_tag="PWY67_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2057647.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5800..6339)
                     /locus_tag="PWY67_001353"
     CDS             complement(5800..6339)
                     /locus_tag="PWY67_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL2057648.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6479..7090)
                     /gene="rpsD"
                     /locus_tag="PWY67_001354"
     CDS             complement(6479..7090)
                     /gene="rpsD"
                     /locus_tag="PWY67_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL2057649.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7352..8203
                     /locus_tag="PWY67_001355"
     CDS             7352..8203
                     /locus_tag="PWY67_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL2057650.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8231..8866
                     /locus_tag="PWY67_001356"
     CDS             8231..8866
                     /locus_tag="PWY67_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL2057651.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8886..9659
                     /locus_tag="PWY67_001357"
     CDS             8886..9659
                     /locus_tag="PWY67_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2057652.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10067..10864
                     /locus_tag="PWY67_001358"
     CDS             10067..10864
                     /locus_tag="PWY67_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL2057653.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10842..11255
                     /locus_tag="PWY67_001359"
     CDS             10842..11255
                     /locus_tag="PWY67_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2057654.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11239..13884
                     /gene="mutS"
                     /locus_tag="PWY67_001360"
     CDS             11239..13884
                     /gene="mutS"
                     /locus_tag="PWY67_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2057655.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13902..16010
                     /gene="mutL"
                     /locus_tag="PWY67_001361"
     CDS             13902..16010
                     /gene="mutL"
                     /locus_tag="PWY67_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL2057656.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16032..16592
                     /gene="maf"
                     /locus_tag="PWY67_001362"
     CDS             16032..16592
                     /gene="maf"
                     /locus_tag="PWY67_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL2057657.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16677..18665)
                     /gene="treP"
                     /locus_tag="PWY67_001363"
     CDS             complement(16677..18665)
                     /gene="treP"
                     /locus_tag="PWY67_001363"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL2057658.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18888..19601
                     /gene="treR"
                     /locus_tag="PWY67_001364"
     CDS             18888..19601
                     /gene="treR"
                     /locus_tag="PWY67_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL2057659.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19678..20124
                     /gene="msrB"
                     /locus_tag="PWY67_001365"
     CDS             19678..20124
                     /gene="msrB"
                     /locus_tag="PWY67_001365"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL2057660.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20294..20896
                     /gene="ruvA"
                     /locus_tag="PWY67_001366"
     CDS             20294..20896
                     /gene="ruvA"
                     /locus_tag="PWY67_001366"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL2057661.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20909..21910
                     /gene="ruvB"
                     /locus_tag="PWY67_001367"
     CDS             20909..21910
                     /gene="ruvB"
                     /locus_tag="PWY67_001367"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL2057662.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21939..22517
                     /locus_tag="PWY67_001368"
     CDS             21939..22517
                     /locus_tag="PWY67_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2057663.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22569..23051
                     /locus_tag="PWY67_001369"
     CDS             22569..23051
                     /locus_tag="PWY67_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL2057664.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23168..23542
                     /locus_tag="PWY67_001370"
     CDS             23168..23542
                     /locus_tag="PWY67_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057665.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7793 a         4291 c         5119 g         6660 t
ORIGIN      
        1 tatttgtctc gcttaattat acaaacggcg aaataaaaaa acttggtacg caatcgctgt
       61 accaagtttt tttatttagc ttttgattaa gccataaata ataatttata taagaatact
      121 gctacgacac cagcacagat aggtgctaca actggtaccc atgcatatcc ccattgagaa
      181 gaacctttat gtttcaatgg aagaagtgcg tgtacaagac gtggagcaaa gtcgcgggct
      241 gggtttagtc caggacctgt aggaccacca agtgaagcaa ctaatgtcca tacaaggaaa
      301 ccaagtgcta agtgagctgt accaagatta tcttggaaga atggtgcttt tgtaatacct
      361 agggcaccaa agaataaaac gaatgaacca aagaattcat tgacgaaacc attgaattta
      421 gaaccaacgc tgttgattgt tgagaatgta cctaacacat ggtttacgtc ttctgtttgg
      481 tcgtagtaag gtttgtaaca agctactacg atcaattgtc caatcgctgc accaatcaat
      541 tgtgcaatga tataaggaac aacttcagcc caagggaata gaccattaga tgctaaagca
      601 attgtaaatg ctgggttgat gtggttaccg ctgattccac caaaaatcat tgctggcatc
      661 ataaccccga atccataccc gacagcaatc agcatccagt cactgccata tccttttgta
      721 ccttttaaat ctacgtttgc tacagcaccg ttacctagaa cgattagtaa tgctgtacca
      781 atcatttccg ccgcaagacg ggtcattagt gagtaatcca taaatacctc caaaagttat
      841 aaaaatgcaa aaaaaaaaga aacatcaaca cgaaaagcat cggtgcagtg tttcgtgtcg
      901 gaaggatttc tgcagtctta acaaatgtga ataaaaaaat gaattccatt acttataaga
      961 aaccagacca acagtaaaac tccaactctt tttaaaagtt ctccacaaat caccaagctt
     1021 aactataaat ataccaaaaa aaaaacgttt tttttctcaa aaattgtatc ttagaaacag
     1081 catgttttga aactttcaga aaaggtttgt tagtaaaatg aaagctatac gtaaggagtg
     1141 ttgtttgatg aaattttttc aagagttttt cattattatt ttcttttctg tattaggaga
     1201 aggcatacgg atttttgctc atttacctat tcctggaagt atcttaggta ttcttttttt
     1261 atttttagca tttgaaatgc atcttgttga tcctgaaaaa atcggagcaa ctggtgattt
     1321 tttattgaat aatctagcca ttttatttgt tcctgctggt gtaggtttat tggaatattt
     1381 tgatgatatt gcttcgatat ggcctgtttt actgggagca gtcgtcgtgt gttccatcgt
     1441 cacgatggtt gcagcgggaa aaacggcaga aggtgtagaa gctttgctgg gctatgtccg
     1501 taagaaaaaa gcagaaaggg tatctgtaaa cgaaatggag gagaatcaaa ttgactgagt
     1561 tttttgtgaa tccattattt ggcttaagtt tgactttagg agtgtacgta ttatctgaca
     1621 aatttttgcg gaaatataaa ctgcctgttt tgaatcctct cgtattatcg attgttgtta
     1681 ttatcttatt tttgcatttt tctgggatca gctataaaga ctataatatc gggggcaatt
     1741 ttttgactat gctgattact cctgcgacag tggcacttgc catccctctt tataaaaact
     1801 tccgtttact gaaagagaat ttttatcctg taatggctgc gattttagca ggaatagtcg
     1861 caaattgctt ggttagtgta gggttcggtt acttcttctc tcttcataaa gagatggtga
     1921 tctcattact acctaaatct gtaacgacgg ctatttctgt cgatttatct catacgatgg
     1981 gcggggtgaa tgccgtgaca cttgctattg ttgtctctac aggtattttt ggttcgctga
     2041 tcgccactca tattttccgc ctattcaaga tcgaaagtcc agttgctcgc ggtgtggcgt
     2101 taggaagtac ttctcatgct attggtacag caaaagcaat cgagattggt gaaatagaag
     2161 gaatcatttc cggacttgcg atatgcgtca acggaatatt gaccgtttta ttgctaccgt
     2221 tattctttca gccttttgct ggcttgtttt gatgcgaaaa ccactatttt atgctacaat
     2281 aaccaaaacg ttcgagcata aaaaaggagg gaaagacgtg tatttgtctt ccgagtatct
     2341 taaacaattt gataaagatc tatattataa gattctcctc ctagaaagtt ttgaggatca
     2401 agcttggcat accgctgcac aattagcgca agttgttcag ctggatgccc gcagcgtgtc
     2461 gaaatacctg aacgagctgt ctaaaaacta tcagcagttt agcggtaaga cacacccttt
     2521 gtttacaaaa aatcaccgtt ctggttataa cttttatgat acgctcgatt caattgaaca
     2581 tgagcgtttt ttgatctatc tcgtgcaaag tacgctgaaa ttccagttgc tgcacgacat
     2641 tttttttgaa gaattccata caatgtacca atttgcgcaa aagcattaca ttagtgaatc
     2701 tactgctcat cgaaaaatca atgaatggaa acagcaactg caaacttacg gaattcggct
     2761 gcaacgaggt acatatatcg ctcaaggcga agaagaaatc attcgacttt acttgcatat
     2821 gacattctgg cagttgtttc gagggaaaat ctggccattt gaaacgattt ctcaaatgga
     2881 cgtaaagaat atggcagaac atatcatggc tttttttaat gttcgtctga acgagatcaa
     2941 aaaaagacga ttggaatata tgctaggtgc ctttttttta agaaaatcac agaagcatta
     3001 tgttgtattg aatgagaaaa aaagacggct gatttctgac aatctgttgt tccagcgatt
     3061 ttgccaggtc atggagccgg tatttcctaa ttattttcaa gtagaagacg agttaggcgc
     3121 attatttctt gtactgatga ctcgtgaaga atattatagt gatcctaaga tacggaagaa
     3181 aatttttgat tttcatcaac aagcaaaaac accgcccttt acagcccttt cagaagcaaa
     3241 agccgctctt tctctttatc aggaagagca ggggctgcca gcagaaaatc taaccttcga
     3301 ggcagaaaac tatttattca gcagtcattt tttcgcctat ctttttccta atgcgaaaga
     3361 aacgattgat ggcaatagta gtgattttat taatcattta gttattgaaa acaaggaatt
     3421 gaaacaatgg ctcgtacatt tttttgaaag tagacacaaa catcccaatc atctggcatt
     3481 taaaaatcat gcgtttttga tggggcggta tctgactgtg tttaaaacat taggagcctt
     3541 tactccacag cttccaaaga tcacgatcct tttaatgaca gatttcccat tatttgaaga
     3601 gcaactgtta gaggagggat tacggacatt tttccgtaat gagtatcagc tgatttttct
     3661 gccaacagat tatcggggga gagaagttga tcttctgatt tcgacaagta aagtccaccg
     3721 caaaccatgg gcggatcttg actattttat tgtgactgaa gaactaaaat taattgatta
     3781 catccaacta tctcaaaaat ttgaaatgat tcaaaaacaa aaactattaa aacaataaca
     3841 aagaaggaga aaaaagatga catttgaaga aattttacca gaattgaaaa aaggtgcaaa
     3901 gattattcgt aaaggatgga gtggatttga actttatgtt gttctcgtat ccgatgatga
     3961 atttgatggc tgtccggtga cgccgtactt attgatcaaa acaagtgacg aaggtttttc
     4021 cagcttcgct cctacagttt gtgatatttt agcagatgat tgggcgatcg tagaatgaaa
     4081 aaccagtatc cagatctagc taaacaggtt gtatttatca caggagctgc ttctggaatt
     4141 ggtgctgcgc aagcccgagc gtttcttgaa caagatgctt ttgtatttgg attagatcaa
     4201 aagttcggta aaatggaaga aatttctttg cgatttcctg aaacttttgc ctacgcacta
     4261 ggtgatgttc gacaaatgaa agaactccaa caagcggtat cgaaatgcca aaatcatttt
     4321 ggtgaagtta cgattttact gaatacagcg gggatattgg atgcctacaa acccttgatg
     4381 gaaactgacg aatcgctgtg ggatctgatt tatgagacga atgtgaaaag catgtatcag
     4441 ctgacaaaaa tcatccttcc tgatatgatc cgccaaaaaa gcggaacgat catcaatatg
     4501 gcttcgattg ctggcttgat tgctggtgga ggtgggatcg cttatacaag cgcgaaacat
     4561 gcgattgtcg gatttaccaa acaactcgct ctggattacg caggacaggg tatccgagta
     4621 aaaggaattg ctccaggagc catccaaaca ccaatgaatg cggctgattt tgaagggaaa
     4681 ggtgagatgg cagagtgggt ggcaaatgaa acaccagtca agcgatgggc acagccggaa
     4741 gaagtagctg agctgacgtt atttcttgcc tctcctcaag caagctacat acaaggaacg
     4801 atcatcccaa tcgacggcgg atggttgtta aaataacctt gtgttttttt caagcttctg
     4861 tatgataaaa ctaaagagag aaacaacggt tgatgataaa aaaagtcgat gactgttccc
     4921 gacattcgat gccgaacagc cagttcaaaa aattcttttt aataatcgtt aatattcgaa
     4981 actaagcggc ttttcacgac ctcttgatat aaacgatatt caaaagcttg caactgcggt
     5041 aacaagtcca gccaaatgaa aaatataagg agctattttt atttgaccat tcttattact
     5101 tgttgcaggg cagctttttc actaccttct gcgacacttc agttggaatt ttcttcccat
     5161 cctttggtcg cggtcattac caagataaaa ttatcttgca aaggagaaat agaacatgga
     5221 aaaaaataat acccgttcag ccaaacgctg gacaacgaca gatgtgactc ggatggcgct
     5281 agtcactggt ttatatgtgg cagtgacgct ggttttgtct gtctttagct ttggagtgat
     5341 tcagattcgt ctctcggaga tgttcaacta tctgtccttg tataacaaaa gatacatcgt
     5401 cgcggtaaca gcgggagtag ctttggctaa tattgcttct cctcttggac tgatagatgt
     5461 tgcagtagga agcgtcagca cgctgcttgt attgcttatc aactataaaa tcactagtcg
     5521 catcaaaaac atgaaaatca aaatggccgt gacagcatgc gtatttgctt tttccatgtt
     5581 tacagtagct ggtcagctaa cgattttata tcaactacct tttttcttga attggtgggt
     5641 gattgcttta ggagaattgg catcaatgat tgtcggtggg ataattattt attggatcgg
     5701 taaaaaagtg gatttgtcaa aataaagatc tgtttaattt aaaatagggc agcatggaag
     5761 agactgaatg ttgagtcttc ttccatgctg cttttttgat taatttgtat gaagaaatcg
     5821 atttagccgt tttttcatca agataccgat ataagctgct ccagttgcct tgattgcttc
     5881 cggcagtaaa aacggcagta gtccactggc aaatgcagtt gtaaaagtaa gatcaccact
     5941 tattttcagc cagatcgttc cgcagatcaa tgcagcaaga gagcccatta gattggcaaa
     6001 gattgcatat aagtaatgag catccgttct ttcgatcatc caaccagtta tccacgtttg
     6061 taagagaaat ccaatcaaat atccgccagt tggcccaaat aaaacagcta tacctccagt
     6121 catccctgaa aatacaggaa gaccaatcaa gccaagtaaa agataaactc caacagatat
     6181 caatcctagt tttcttccga gaaaagttgc tgttaatcca atagcaaatg tttgccctgt
     6241 caaaggaacc aaaccaaaag gaatggtcat ctgagaaagg acagaaataa tcgcggcaaa
     6301 ttccgcagca agtatcaatt ctttcaatgt tcggttcata acgccctctt ttcgttaact
     6361 taatattttt atttggttaa cgaaattgta aaacaaagtg attactttgt aaatagaaaa
     6421 tataaaaaaa gaccagctat ccaatagata gcggtctttt tcaagaaagc ttttgaaatt
     6481 ataagctttg gttgtagtat tcaacgacaa gagcttcgtc gatttctgga tatagttcgt
     6541 cacgttctgg taaacgagtc aatgaacctt ccaatttttc agcgtcaaag cttacgaatg
     6601 ctgggcgtcc aacagttgct tctacagctt cttttactgt tacgatgttt tgtgattttt
     6661 cacgaactga gatcacttga cccacttcta cgtggtatga agggatatca acgcgtttgc
     6721 catcaactaa aatgtgacca tggttaacta attgacgtgc ttgacgacga gtcgttgcaa
     6781 gtcctaaacg gtaaacaaca ttgtctaaac gttgttctag taagatcatg aagttaacac
     6841 catgtttacc ttctttgatt ttgctagctt ttacgaacaa gttacggaat tgacgttcgt
     6901 tcattccgta catgtgacga agtttttgtt tttcagttaa ttgcatgccg tattctgatt
     6961 gtttgccacg gctattaggt ccatgttgac ctggtgcgta agggcgacgt gctaattctt
     7021 ttcctgtgcc tgacaatgag atgcctaagc gacgagaaat tttccatgat ggtcctgtat
     7081 aacgagacat taaaatttcc tccaatgaat ataagttttt ggagtaaaat aatccgttga
     7141 aaaattcata atcatatagt ttgttcttca atcttcacct ttgcagccgc ggttacacaa
     7201 tcgaacccga gacggggcaa caaactaggg atgagttatt atttttctgc tgcattattt
     7261 tacacaaatt acagtatatg atagatcatc cacgaatgtc aagataatta gtacataaaa
     7321 acgctttaca aacctaggct gtctttataa aatgagaggg tatgtgcaaa tggaaaaaag
     7381 cgaaggaagt ttaaggagaa aatcaatgaa tccggtatca ccattatttg agaaaatcga
     7441 tgcttcaatt gaaaagaagg cgaatctttt tgaaaacagc ttttcacgtt acgctgttcg
     7501 agcggttctc gcctgtttgt ttttgacttt aggaacggct gtggcatttg cgatcgcgat
     7561 gaagggggaa gatatcgcgc atggtctagg aaagatttta tacgcattta tgttcagctg
     7621 gggtctggtc atgattctat acatgaacgc cgaattagga acatcgaata tgctttatat
     7681 gaccgtgggt gtttttcggc agagacttgc tttaagtaag gcagccaaga ttctatttgc
     7741 ctgtattttt ttcaatatga ttggcggtat attatgtgga tttctaattt ctatgactac
     7801 gccatttcaa gatttaacgt tggacaactt tatgttagaa tcaatagcag taaaactagc
     7861 aaaaccttca gcaacgattt tggtggaagg gatgtttgcg aatatcgttg tcaataccgc
     7921 tgtactgatc agcatgcgga tgaaagagga tgtcggaaaa gtgatcacag tcgtattcat
     7981 cattttcatt tttgcttttt taggatatga acacgtgata gcgaattttc ctgcctttac
     8041 gcttacttac tttgcttctc acggacagat ggatggaatg acagtcagca atgtgttaca
     8101 taatttgttt tttgctttgg caggaaatta tattggtggc ggtttagtaa tgggactggg
     8161 atatgcttgg ctgaatcagt caaaatcatc ctatgtggac taaaaagtcg ttcattaaag
     8221 gggaagtgcc atggaagttt tagtttttcc aaaaaatgag gaaaaagcgt tacaaatgga
     8281 aaagtacatg aaaaatcttt tttcctttgc tggagtacct aagccggaac gcgctctttt
     8341 acaacaaccg ttactggatg agagcaaaaa tttatctatt tctgaagttt tagcttttgt
     8401 tcattattat tatgaacaaa tcgaaagaga gtatcagtat gtagcgatcg atttagtcca
     8461 gaaaaacatc aaacggctta catttgaaga cttagtcgta ttgttgccac tagttcagaa
     8521 aaaagaatgg tgggacagca tcgactcttg gagaaaagta tacagcacat ggtgtaaagc
     8581 ctacccagag cagcgtgaag aagtctttca attgtttgaa aaaaaggaag atttttggct
     8641 acgtcgaata gcaatcacgt tgcaattagg ttataaacag caaacagaca caggactatt
     8701 gatccgagca atagacgaag atcgtcaaac aaaggaattc tttatccaaa aagcaattgg
     8761 ttgggcatta cgcgattata gtaagaccga tgccgattgg gtaaaaagac agctgacaac
     8821 agatttgagt aagttagctg ttcgagaggc aagtaagtat ttataagaaa gcgagacgac
     8881 aagagatgag acgcaaatta tttgcttttg atattgatgg aacgttgtta gattcaaaca
     8941 aacaagcttt agatagcaca agagaagcat tggaaaaatt acgcaaagcc ggtcatctgg
     9001 tcacgattgc tacagggcgg agccgatttc atgcgcaaga gatcattcgc gatcttgatt
     9061 ttacgaacta cattttatgt aatggagcgg ccggtttttt agaccatgaa caagtatata
     9121 agaatttact agatcgcgac cagttggatg cgttcgttca agaagcgcat catgagcaga
     9181 tcgatacagc ttttgtcagc ttagacaata tcaagcgttc ttcttccaat gatgtaacgg
     9241 tgatggaaga agcgatgcat tctttcggtg ctgtcttgcc tgaattagat cagttttttg
     9301 cagaggaaca agaaatatac caagcgttag catttttcga tcaaacatat gatgggaagt
     9361 tctcttcata tgataaaatt cgatttgtcc gctggcatga gaacagtgta gatgtcgtac
     9421 ctaaaaatgg ttctaaagca gcgacaattc tgcacttagc agaacaagta ggcatccctc
     9481 atgaagacat catcagcttt ggcgatggac aaaacgaccg tgaaatgttg cgcatgtcag
     9541 gaattgggat agctatgggg aatgctgtgc cagaagtaca agcagaagct aagatggtca
     9601 ctgatacaaa cgatcaggat ggaatttgga aggcattgaa agagttaagc gtgatttaaa
     9661 agaggttgtg aaaaagctgt tctgcatcaa gtaataagcc cagccaaaca aaaatagctc
     9721 ctcctatttt tcgtttttct gggcttatta ccgcagatgc aagctttcga acaccgttta
     9781 aggaagaggt cgtgacagaa gtggtcagtt tcaagaaata agaagggatt tacgaaaatt
     9841 gcttctcaaa tttttgtaaa attacggctt atttctgaag aagtagatga aacaacgttt
     9901 attcgggaaa tgggactgtg acaagatgtt tgtcatagtc ccattttttt attttatttt
     9961 tcaaaactaa gaaataccag gaaagagttc cttttgtttg accttttctt tcttattgaa
    10021 aacaactata atatagagtg atgttaaaag agaagaggaa tttagtgtgc gggtattgtt
    10081 tattggtgat gtagtaggtt caatgggacg agagatgatt actgaatatc ttcctagatt
    10141 gaagaagaag taccgtccgc aagtaacgat tgtaaatggt gagaatgccg cttctggacg
    10201 tggaatcaca gaaaaaatct ataaaaaatt tttgcaagac ggcgtagatg tcgtgacgat
    10261 ggggaatcat acgtgggaca accgcggaat ctttgagttt gtaaatgaag ctaaaaagat
    10321 ggttcgtccg gcaaattttc cggaagggac gcctggacaa gggatggtct ttgtcaaagt
    10381 gaaccaagtc gaactggcag tgatcaacat gcaggctcgt tcttttatgg tggacttgga
    10441 tgatcctttt cgtaaaatga atgagctggt agaagaagcc agaaaacgta cgccgatcat
    10501 ttttgtagat tttcatggag aaacaacaag tgaaaaacaa gcaatgggct ggtttttaga
    10561 tgggaaagtt tcagcagtgg tcggtacaca tacacacgtt cagacaaatg atgcgcgaat
    10621 tttgccaaga ggaacagcgt atctgacaga cgttgggatg acaggtccct atgatggtat
    10681 tttgggtatg cgcagagaac ctgtaatcga aaagtttctg actgcattac caaaacgttt
    10741 tgaagttgtg gaacaaggcc gatcgatttt aagcggctgt atcttggaat tggatgatac
    10801 gacaggtcat gcaaaagaaa tccagctgat tcagatcaac gatgatcgac cgttcatgga
    10861 gtagaaagag aggcagtttt ttgcagcagg agccggaaat tcaaaaagaa gtaaggaaac
    10921 tgacaaaact tttaagagag aatgaaacga tcattcgcta caaagaactt gaagaaaaga
    10981 tccagcaaaa ccagtattta gctgagttga gagaaaaaat caaacaagcg caaaaagatg
    11041 ctgttcattt tgcccactat gacaaaccag cagcagaaaa agaagcaatc aaacaagcgg
    11101 atcaattcat gcaagaattt gatcagcatc cgcttgttgt agcttataga aaacagctgt
    11161 tggaagcaga tgatctgctt catcatttaa caactatgat acaagaagaa atcaatggac
    11221 aaatagagga ggaaaaacat gcctcaaaaa actaaaaata ctcctatgat ggaacaatat
    11281 ctaagcatca aggcccaata ccaagatgct tttttgttct atcgtttagg agacttttat
    11341 gaactatttt acgaagatgc gatcaaagcg tcacagcttt tggaattaac gttgacgagc
    11401 agaaatcgaa atgcagaaga acctattcct atgtgcggtg tacctcacca tgcagcacaa
    11461 ggatatatcg atgccttgat tgaaaaaggc tataaagtag cgatttgcga gcaggtagaa
    11521 gatccaaaga cgactaaagg gatggtcaag cgcgaagtga tccagctagt cactcctggt
    11581 acagtcatgg acagcaaagg attgtcggca aaagacaaca attttttaac agctgtagtt
    11641 agtcaaggaa atgaatttgg ctttgcttat gccgatttaa gtacgggaga attgaagacg
    11701 gctcggttgc atgatgaaga agctgtttta aatgaagcct ctgcattgca gacaaaagaa
    11761 ctcgtattag gcagtgagat cactgatacc cttcgtgaac agctaagcgg gcgtttaggg
    11821 ctagtctttt ctcaacaaaa tgagatggaa gaaaacgcgg aatttagctt tttgacaagc
    11881 gaactagtcg atccgctgga aaaagaagca accggcaaac tattgactta tttatcggtg
    11941 acacaaaagc ggagtttagc tcatatccaa aaagcagtag aataccaacc agatcatttt
    12001 ttgaaaatgg attattattc aaaattcaac ctagaattaa gccaatctat tcggactggg
    12061 aaaaagcacg gcactcttct atggttgtta gatgaaacaa aaacagcaat gggtggccgt
    12121 ttactaaaac aatggctaga ccgtccgctt atccaggcaa aacaaattaa agcaagacaa
    12181 gaaatggtcg cttctttgct agacagctat tttgaacgta tcgatttgca gtcagcatta
    12241 acgaaagtct atgatctgga acgcctagca ggtcgagtag ctttcggtaa tgtcaatggg
    12301 cgagacttga tccagctgaa aacttcttta gaacaagtgc caatgatcag agggttgatt
    12361 gaaggaatcg atcaaggtca atggcaaagt ctgctttcag aaatgcagcc gatggaccat
    12421 ctcgtccaat tgatcgacca agctatccaa gatgatccgc cgttacaaat aacggaagga
    12481 aacatcatca aagatggatt caatgagcag ctcgatatgt accgttctgc tatgagaaac
    12541 ggaaagaaat ggttggcaga acttgaagca aaagagcgtc aagaaacagg aatcaagaac
    12601 ttaaaaatcg gatttaaccg cgttttcggt tattatatag aaattacaaa agcaaatctt
    12661 ggaaatgcag aactggagaa gtatgaacga aaacaaactt tagcaaatgc agagcgtttc
    12721 atcacaccag aactaaaaga attggagaca caaatcctcg aagcagaaga aaaatctgtt
    12781 gatttagaat atcagttgtt tttagctgtg agagaagaag tcaaaaaggc tatccagccg
    12841 ctacaagtac ttgcaaaagc aatcagtgca gtagatgtgc tacaaagttt tgcaacgatc
    12901 agcgaacgct atcagtatgt tcggccagaa cttgtcagtg acaagcatca gcttctgatc
    12961 aaagacggac gacatcctgt agtagaaaaa gtattaggac atcaggaata tatccctaac
    13021 agcgtagaaa tggcagaaga cgaaatgatc ttgctgatta caggaccgaa tatgtctggt
    13081 aaaagtacgt atatgcgaca gttggcatta actgttctga tggcgcaaat gggctgtttc
    13141 gttccggcaa aacaagcagt tttacctatt tttgatcgga tttttacccg aattggtgca
    13201 agtgacgact taattgctgg acaaagtact tttatggtag agatgatgga agccaatcaa
    13261 gccctgcgtt atgctacgcc aaacagtttg attttgtttg acgaactagg gcgaggaaca
    13321 gcaacctatg acggaatggc gttggcccaa gcaatcatcg aatatatcca tcgtcatgta
    13381 caagcaaaaa cactcttttc tactcattat catgaactga cggtacttga gaaagaactt
    13441 ccgcagctta aaaatgtcca tgttggagcc gttgaaaaag acggagaagt tgtcttcttg
    13501 cataaaatga tggatggacc tgcggataaa agctacggca tacatgtggc aaagatcgca
    13561 ggattgcctt ccaatctact agaacgagca gcagatatcc tgcatacgct agaaaatggc
    13621 gataacggac accatactgt tccagctgaa gtagaagaga aaactaaaac agccaaacag
    13681 gagcaaataa aaccacctgc agaaaaagta aatcaagacc atatacaaga ccgtgtaaaa
    13741 gaagaaaacc aacagctttc tttattcagt gaattatccg aaaatgaaac agaagtcatt
    13801 gaatcactga agcaaataaa tttactagaa atgacgccaa tggatgcact aaacacatta
    13861 tatgaattac aaaaacaact gtaggaagaa ggcggatatc gatggccaaa atccaagaat
    13921 tatctgaacg tttagctaat cagatcgctg cgggagaagt agtggagcga ccagcttccg
    13981 ttgtgaaaga attagtggaa aatgcaattg acgccggcag tactcagatt gatatcttag
    14041 ttgaagaagc gggactgcgg acgatccaag tgattgataa cggagaaggc attttggcag
    14101 acgacgtaga gaatgccttc aagcggcatg cgacaagtaa gatccataat cgcgacgatc
    14161 tttttcgcat acgaacgctg ggctttcgag gggaagcttt gccaagtatt gcctctgttt
    14221 ctgaaatgac tctggaaacg gcgaatcagg aagaaaaaca aggcacctat ctgtcgctga
    14281 aaggcggaga agttattgag catcgaccag caccactgcg tcaaggaacg aagattaccg
    14341 tcagcaatct gtttttcaat acgccagccc gcttgaaata cgtgaaaaca ctgcacacag
    14401 aattagcgaa catcggggac atcgtcaatc gactggcatt gagtcatcca tcgattgctt
    14461 ttcgtttagt acatgatgga aacaaaatgc tggcaactgc tggaaatggc gatctgaaac
    14521 aaacaattgc gggaatctac ggattatcta ccgcaaaaaa aatgctgaaa atcgaaggaa
    14581 aagatctaga ttttgagtta tttggctatg tttcattgcc tgaggtgact cgtgccagta
    14641 ggaattactt atcgacgatc atcaatggac gttttatcaa gaacttttcc ttgaataaag
    14701 caatcgttgc aggttatggg tcaaaattaa tggtcggtcg tttccctatt gctgtattgg
    14761 aaatcaaaat ggacccgctt ttagtagatg taaatgttca tccgacgaag caggaagtcc
    14821 ggctgtcgaa ggaaaaagaa ctaacagagc taattagtaa cgccattcaa aaagcattgt
    14881 ctcaagaaaa tctgattcca agtgcagcgg ataatctacg gtttaagaaa aaagtacctg
    14941 atcagcctaa aacagaacaa atcgaaatcg atttgtccta cgcttttgaa gaagaaaaac
    15001 ctaaaaagcc aagtagtttg aattttgatc gagagacagg tacctttttc gtagaagaac
    15061 taaagaacac atttgttgac agaattgttg ataagtctgt ggaaaatgtt gaaaactatt
    15121 ctggtttttc tgaagaaaac accacttatc cagaaaaaac agcgaaaacg gtggataagt
    15181 ttggtgaaaa tgttgataac tttgtagaaa aaacagtgga aaaatctgaa aagaaagaat
    15241 ttcccattgt ggataacatg gagaaaacaa atgaagaaca agcagataaa tccgtagaag
    15301 aggatgaaaa gatttacgag gaagaattga cactccatcc tgaatttgat ttcactcaag
    15361 gaaacgacaa aacattagaa caagccatca agaaattaga aacggaaaaa ccagtacaac
    15421 gttttcctca gttggaatat tttggacaga tgcacggaac gtatcttttt gcccaaagta
    15481 gcgatgggtt gtacatcatt gatcagcatg ctgcccaaga gcgcattaag tatgaatatt
    15541 tccgggaaaa aattggggaa gtatctgatg atctgcaaga attgcttgtt ccatttgtgt
    15601 tagactatcc aaacagtgat gctttgaaat taaaagagca aaaacacttg ctagaagatg
    15661 cagggattta tttagaagaa tttggtcaaa acagctttat tgtacgggcc catcctactt
    15721 ggtatcccaa tggagaagaa gaatcgatta ttcgggagat gatcgatatg ctgctgacaa
    15781 ctggaagtgt gagtgtaaaa aaattccgtg aagcaacagc aatcatgatg agttgcaaac
    15841 gatcgattaa agccaaccat tatttgaatg aagcacaagc acgtgtgctg ctgaaggatc
    15901 tggcacgatg cgaaaatcct ttcaattgtc cccatggccg tccagtattg attcacttta
    15961 ccaattcgga tatggaaagg atgttcaaac gaatccaaga cccgcattaa gtcaaagaga
    16021 agagggaaaa catgaagatt gtattagcat cccaatcacc aagaagaaaa gagttattgg
    16081 ctcatatcgt ttcagaattc gagattgaac ctgctgatat cgatgaaacg ccatttccag
    16141 acgaacagcc cgtcgattat gttcgtagaa tggctgaaga aaaagcacga actgtctggg
    16201 aacaagatga aaaagaagac catttagtga ttgccagtga cacgactgtt gttctcaatc
    16261 aagagatcat gggtaaacca gaagatctgg ctgaggctga attcatgctg aaaaaactaa
    16321 gcggggaaac acatactgta tatacagctg ttgttttaaa aacaaaggaa cgagaagaac
    16381 ggatactagc agaagcacat gtcacttttt atcctttgac agacgaagag atcaaacgct
    16441 acttggagac gggggattat gcggataagg caggggcata cggcatccaa aatgctgcga
    16501 gtgtattcgt caaagaaatt tctggagatt attacagtat cgtaggattt ccgattggtg
    16561 ctgtcaatca ggcactgaaa acattcattt aaacaaaaaa gcggactatc tgattttcga
    16621 ttgaaaatcc atgatagccc gtttttttta ttgaaaagat tacttatcta ctctgactaa
    16681 gattttactt cagctttacc gactgcttcg cccgggcgaa caagtcctga ttggtcaaga
    16741 gaaagtcctg caactttttc attattggtc aaagcaacaa taatgtcagt tttcttgcca
    16801 gcttgttcta tttctggcaa atccatttca gcaatcaggg tctctggtgt gacaaggtat
    16861 ccttctttca cgaatacatt gaaaggaaca ccctttagtt caacagtatc taatcccatg
    16921 tgaacaagta cttccagccc ctcattagaa agaataccaa tggcatgttt tgtttcaaag
    16981 acactcgtca ctttaccgtt tactggtgca tagactttgc cgtttgacgg ttcgacagca
    17041 taaccgtcac ccatcatttt ttgtgagaaa actgggtcat ttacttcagt tatttccttg
    17101 atggtccctg cagcgactgc gaataatgtt tcttttgttg caactgccgt accagttgct
    17161 gaaacaggtt cgaaagactg tggcgttgct gttgcgccat ttgctgtttg aagttgtaca
    17221 tcagcagcat tttcaggaac agcaggatca atcttattca aaattccctg acgtctaaag
    17281 atgatcgtca agccaaatgg aatgatgaca gcaatgatca ttgcgatgat gaaaggtacc
    17341 catgtttctg cttggatagc tagtattcct ggcaaaccgc ctactccgat cgcgttagct
    17401 cgaacaccca tcaagttagc aaacatgcca gctaatccag agccgaccat tgcagcgacg
    17461 aaaggataga cgtatttcaa attgattccg aacatagcag gttcagttac gcctaagtaa
    17521 caagaaatca ttgctggaat cgagatttgt tcttcttttt tattcccacg atgtaagaag
    17581 atgatggcta agactgcaga gccttgggcg atatttgata aagcaatcat cggccataaa
    17641 ttcgttgatc cgaaatctgc aatcagctgc atatcgatgg cattggtcat atggtggaga
    17701 ccagtaatca ctaaaggagc atacaggaat ccgaatacgg cactaaacaa ccagctgata
    17761 gaagacgtca atccagtatt tacgatattt gagatccagc ttccgactgt ccagccgata
    17821 ggacctaaaa taacgtgtgc agctaaaaca gttggtaaca atgaaaacaa tggaacaaaa
    17881 atcatcgaga tcgactgagg gatatatttg cggaagaaga tttctagata cgctaacata
    17941 aaccctgcca gcatggctgg aataacttgc gcttgataac caatcatctg gacttgagca
    18001 aaaccaaaat cccaaaatgg aatatctgct gcggctgtgg aagctacact ataagcattt
    18061 aataattgcg gagaaactaa cgtaattcct aaaacgattc ctaatatttg tgtagtaccc
    18121 atcttttttg caatactcca agtgatacct actggaagaa aatggaaaat tgcttcaccc
    18181 ggtaaccaca aaaaggcatt gacaccattc caaaacttcg agtgttcaac gattgttcca
    18241 ccaagacttt caaactggat accttcaagt acatttctga atccaaggat caatccaccg
    18301 acaatgattg caggaatcag tggtgtaaag atttctgcta aaacggcaat agcacgttga
    18361 acgggatgaa ggttttgttt tgcagctgct tttccttgtt ctttggaaac cccttcaact
    18421 ccagatactg caacgaagtc gttataaaaa gtagaaacat cgtttcctat gatgacttgg
    18481 aactgtcctg cattcgtgaa catccctttt acgctaggta tatcttcgat cgctttttcg
    18541 tccgcttttc ctggatcatt caggacaaaa cgcatacgag tcgcacaatg actaactgca
    18601 gcaatatttt cttttccgcc gatatcatgt aacagcttct cggcatctgc ttgatatttt
    18661 gccatatcaa taaacccctt tctcttaact tgtctatgca agttgtttct tcatttacta
    18721 tacttttttt cggagagaat tgcaagcgtt ttattaaaaa gtacgagaga aaatgatatc
    18781 gttttaaaaa gcttaaaata agcttttctt aaaaatagga acattgaaat acaaaaagag
    18841 ctgtagtata gtgaagaaat gatggaagaa cgagtgaggg aaaaaacatg aataagttca
    18901 atgaaatttt tttagatttg gaacagaaaa ttttagcgaa agaatatcct ccgcattcgc
    18961 ttttacccag tgagaaccaa ttgatcaaaa tttatggtgt ttcacgagaa acaattagaa
    19021 aagcgctgaa tcttttgact acggaaggtt atattcaaaa aaaacaagga aaaggttcga
    19081 tcgtactgga tcggaatcgc ttcgactttc ctatttctgg attgactagc tataaagagc
    19141 tacaggagac ccaacgtatt ccaagtgaaa cgatcatcca tacgttagaa gaaacagaag
    19201 tgacaaaagc attgaacgag atcactggct gggagatcgg tgcgcctgtt tggcatttga
    19261 tacgcgaaag aaaaatcaat ggcgaagtag ttatattaga caccgattat ctattaaagg
    19321 aaatcgttcc tattttaact ccagcacaag cacaaggatc gatctatgaa tattttgaaa
    19381 atgagctttc tttgacgatt gcttacgcgc aaaaggagat cacagtagaa gaagtaacgg
    19441 aaatcatgaa aacagcgatg gatcttcacg atgacactta tgcagtagta gtcagaagcc
    19501 tcgttcatct ggaagatacc cgctgtttcg agtattctga gtcggttcac cgattagata
    19561 aattccgttt cgttgagttt gcccgacgcc gaaaagtgta aatagttcaa aagaaagcaa
    19621 attgttggca tctgtccact ttggcgctat gataggatca caaagggggc atttttaatg
    19681 gataaaccaa taagaccaga aaaagaagaa ctgaaagaaa agttatcacc catggcttat
    19741 gcagtcacac aagaaaatgc aacagaacga ccatttagcg ggaaatatga tgatttttac
    19801 gaaaagggga tctatgtaga tatcgtcagc ggtgaaccgt tattcagctc tgcagaaaaa
    19861 tatgatgcag gttgtggctg gccatcattc agcaaaccaa taacgaaaag acaagtgcgc
    19921 gaaaaagccg atttttctca tggcatgcat cgaatagaag ttcgaagcaa agaagcagat
    19981 tctcatctcg gacatgtgtt taccgatgga ccagtagatc aaggtggtct acgttactgc
    20041 atcaattctg cctcactgaa attcattcca tatgaaaaga tggatgaatt aggttatggg
    20101 gaattcaaat cacttgtaga ataatggaaa tacacaaaga gcggtacaat acggggattt
    20161 actcgatcgt ccgctctttt tttattcaag cgattgctat ttgaaagaaa cgctccctag
    20221 actccttttt tcacaaggtg aaatgtgcta caataaaggg aacgtatgtg tgcaaaagaa
    20281 agaggactag aatatgtatg aatatctgac aggtgtggtt acctttatca acccctatta
    20341 catagtagta gaaaccaatg gtatcggcta ccagatcgca ctaggaaatc cttaccgcta
    20401 cagtagcaaa ctaaatcaaa aaataaaaat ttatgtccat caggtaatac gtgaagatgc
    20461 gcatcttctg tatggatttg attcgctaga agaaaagcaa ctgtttttgc gattagtaag
    20521 tgtttcagga atcggtccga aaagtgccct tgcaatcatg gcaagcgaag accactccgg
    20581 tttgattcaa gcagtagaat ccagtgatgt cacttatttg acgaaatttc caggagtagg
    20641 gaaaaagaca gcacaacaaa tgatcttgga tctaaaagga aaattcggtg agctgagtat
    20701 cgatacacct tttagtctat ttgatgaagc aaacacaaaa gacgctacag ctctatctga
    20761 agcaatagaa gctctctcgg ctcttggtta cagtgatcga gaaatcaaac gtgtagaaaa
    20821 acaattaaaa gaagtagata atcagacaac ggatgagtat ctacgccaag cactcaagct
    20881 aatgatgaaa aaataaggag gagaccaaat gacagatgaa tacttgttat caccagaatc
    20941 tggcgaaaat gaactgagtt tggaaaaatc tcttcgacca cagctgcttt ctcaatatat
    21001 cggacagaga aaagtcaaac aagaacttca gatttacatc gaagctgcca gaaatcggga
    21061 agaaccttta gatcatgttc tgttgtacgg gcctcctgga ttaggaaaaa cgacgatggc
    21121 catggtcatt gctaatgaga tgaccgtaaa tattcgtacg acgagtggcc ctgctatcga
    21181 acgtccgggt gacttagtag caatcttgaa tgaactggaa gcaggggatg tcctgtttat
    21241 cgatgagatc catcgattac ctagagtggt agaagagatg ctttattcag ctatggaaga
    21301 tttctatgta gatatcatgg taggacaagg gccaaccgca catccggttc atttccctct
    21361 tcctcccttt actttgattg gcgcgacgac acgggcaggt atgctttctg cacctttacg
    21421 agatcgattt ggcatcattt ctcatatgga atattatgac gaacaggatc tgcaggagat
    21481 cgttttacgt tcttcggata tttttcaaac agagatcatt gaagaagggg cattggaaat
    21541 tgctcgccga tcacgaggaa cgcctagaat cgctaatcgc ttgctcaaac gggttcgtga
    21601 ttttgcgcaa gtccaaggag acgggaaaat cgatcgtcct atagcggatc aggcacttac
    21661 tttgctacag gtcgatcagg ctggattgga ttatgttgac cagaaattgt tgcgaacaat
    21721 gatcgagtta tatggcggcg gaccagtagg gttaagtacg ctttcagtaa acataggaga
    21781 agagcgtgag acggtagaag atatgtatga accgtatctg atccaaaaag gatttttaaa
    21841 gcgtacacct agagggcgaa ttgctactgc ttatgcctat gaacattttg gttacgacta
    21901 tcatatatga tttttgcttt tagattggag gaaaatgaat gagtgataca acacgtacga
    21961 aacaattgat caaaaacgta ttgattgatt tgtgccaaac gcaatctttt cgaaaaatca
    22021 gcgtccaaaa aatcgcaaac gaagcaaaaa tcaatcgaca aacattttat tatcatttta
    22081 cagataaata cgatttgctt cgttgggtct attgtgagga tgcattgcat tatttagatc
    22141 cacaatgtct ttcacttgat aattgggaag agcaagcatt gaaaatgctg aaagcaattg
    22201 ctgagaagaa agaattttat gcaaatactg tgctttctga cccagaaatt ttacaaaaag
    22261 agttttctca actgatccaa aaatcattta tcaagatttt tgaacaaatg gatgaagaaa
    22321 gacaactttc tgcaagagac aaactatttt atgctcgttt tttttcttac ggttgcagtg
    22381 gtgtattagt caattggatc acgaatgaat atgaagaaac accgatggaa atcgctatgc
    22441 agcttttccg tttagccaag gatacggaat tatattctta tcgtctttat gcaaaagaag
    22501 agttggaaga acagtaatag aaaaattttt tgttggaaaa gcaataatcg ctaaaaggag
    22561 atggggaaat gacaaaaatt ttatttgttt gtttgggaaa tatttgccgt tcaccgatgg
    22621 cagaaggctt gctgagaaaa cgaatagcat tagaaggaag ggaagcagaa ttttttgtgg
    22681 attctgctgc tacaagcacg tatgaagttg gaaagacacc gcaccctgga acgaaaaaaa
    22741 tattaaacca agaaaaagta gatatgacga atatggttgc tcggcaaatc acaccccacg
    22801 attttgaaac ttttgattgg atcattggca tggatcaaga aaatgtagaa gaactgaaac
    22861 gaagagcacc acgctcagcg caggggaaaa tccatttgtt tttaagtagc attcctggaa
    22921 aagaaaaaga aaatgttccc gacccatact ataccaataa ctttgatgag acatatcact
    22981 tgatcacaga aggcctgaat cattggtgga atatttggat tgatgaaaat aaaataacaa
    23041 ataatttgta aagttaacgc tttcttttta aggcgatgtt agagcatttc cttgtttttt
    23101 tgtaaaatgt taggtaaaat aaaattaatt aagtaacact ttcttagcta aaggaggagt
    23161 gcattcgatg aacgaagaga cagtatcgcc aatgaagaag cttacgaagt tagttactca
    23221 tacttcgtat caactttttg attcttttgt aaatgacact gaagaacatg ctaagaatgt
    23281 acgattgcaa ggaaagttta ttcttgagct gcaaaaagct gctactgaac atagtctagt
    23341 tatcttacaa gtaagagcaa atcgtttgag tagcaagtat gaaacgatct caggctgggt
    23401 ggcgacaaaa acggtcgaaa aagaccgagt gatgatccgc ctacaaaacg aagagcagca
    23461 gatccggatg gttcctgtag aacaaattga aaaaataaca atcctttcac cagcagggga
    23521 acaggaacgg ttatcaaaat aatttatacc agatagaaaa gcacgtatga gcttgtacca
    23581 tcaatcatta ctaatcaaaa ataaccgaat gctttaagct gatatcagta aaacggaacg
    23641 ttttaggatg agtatcatag catgacagca ttcggttgat tagttgaatc gattggatgg
    23701 tacaagtttt tttgataaaa aaaggtcctg ctagccgggg agctagcagg ataaggagtt
    23761 aaaaatgaaa aagtgttgtt agggttgttt gttggtatgc ttatataata gtcattagtt
    23821 gtgaatattt tgtgacgaaa gttttcgaaa gttgttaagt ttt
//
LOCUS       DANKAZ010000036        23489 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000036 DANKAZ010000000
VERSION     DANKAZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(89..2029)
                     /gene="thrS"
                     /locus_tag="PWY67_001371"
     CDS             complement(89..2029)
                     /gene="thrS"
                     /locus_tag="PWY67_001371"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL2057666.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(2366..2545)
                     /locus_tag="PWY67_001372"
     CDS             complement(2366..2545)
                     /locus_tag="PWY67_001372"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL2057667.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(2592..4415)
                     /gene="cadA"
                     /locus_tag="PWY67_001373"
     CDS             complement(2592..4415)
                     /gene="cadA"
                     /locus_tag="PWY67_001373"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2057668.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            4693..6972
                     /locus_tag="PWY67_001374"
     CDS             4693..6972
                     /locus_tag="PWY67_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2057669.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(7065..7712)
                     /locus_tag="PWY67_001375"
     CDS             complement(7065..7712)
                     /locus_tag="PWY67_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057670.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(7891..8610)
                     /locus_tag="PWY67_001376"
     CDS             complement(7891..8610)
                     /locus_tag="PWY67_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2057671.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(8741..9892)
                     /locus_tag="PWY67_001377"
     CDS             complement(8741..9892)
                     /locus_tag="PWY67_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL2057672.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(10109..10849)
                     /locus_tag="PWY67_001378"
     CDS             complement(10109..10849)
                     /locus_tag="PWY67_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2057673.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(10853..11176)
                     /gene="rsfS"
                     /locus_tag="PWY67_001379"
     CDS             complement(10853..11176)
                     /gene="rsfS"
                     /locus_tag="PWY67_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL2057674.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(11233..11826)
                     /gene="yqeK"
                     /locus_tag="PWY67_001380"
     CDS             complement(11233..11826)
                     /gene="yqeK"
                     /locus_tag="PWY67_001380"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL2057675.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(11810..12460)
                     /locus_tag="PWY67_001381"
     CDS             complement(11810..12460)
                     /locus_tag="PWY67_001381"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL2057676.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(12470..12781)
                     /gene="yhbY"
                     /locus_tag="PWY67_001382"
     CDS             complement(12470..12781)
                     /gene="yhbY"
                     /locus_tag="PWY67_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL2057677.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(12797..13906)
                     /gene="yqeH"
                     /locus_tag="PWY67_001383"
     CDS             complement(12797..13906)
                     /gene="yqeH"
                     /locus_tag="PWY67_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL2057678.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(13903..14430)
                     /locus_tag="PWY67_001384"
     CDS             complement(13903..14430)
                     /locus_tag="PWY67_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL2057679.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(14610..15395)
                     /locus_tag="PWY67_001385"
     CDS             complement(14610..15395)
                     /locus_tag="PWY67_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL2057680.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(15388..16257)
                     /locus_tag="PWY67_001386"
     CDS             complement(15388..16257)
                     /locus_tag="PWY67_001386"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL2057681.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(16258..17637)
                     /locus_tag="PWY67_001387"
     CDS             complement(16258..17637)
                     /locus_tag="PWY67_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL2057682.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(17645..18064)
                     /gene="fabZ"
                     /locus_tag="PWY67_001388"
     CDS             complement(17645..18064)
                     /gene="fabZ"
                     /locus_tag="PWY67_001388"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2057683.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(18089..18565)
                     /gene="accB"
                     /locus_tag="PWY67_001389"
     CDS             complement(18089..18565)
                     /gene="accB"
                     /locus_tag="PWY67_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL2057684.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(18568..19803)
                     /gene="fabF"
                     /locus_tag="PWY67_001390"
     CDS             complement(18568..19803)
                     /gene="fabF"
                     /locus_tag="PWY67_001390"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL2057685.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(19822..20559)
                     /gene="fabG"
                     /locus_tag="PWY67_001391"
     CDS             complement(19822..20559)
                     /gene="fabG"
                     /locus_tag="PWY67_001391"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL2057686.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(20571..21488)
                     /gene="fabD"
                     /locus_tag="PWY67_001392"
     CDS             complement(20571..21488)
                     /gene="fabD"
                     /locus_tag="PWY67_001392"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2057687.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(21595..21819)
                     /locus_tag="PWY67_001393"
     CDS             complement(21595..21819)
                     /locus_tag="PWY67_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2057688.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(21872..22834)
                     /locus_tag="PWY67_001394"
     CDS             complement(21872..22834)
                     /locus_tag="PWY67_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL2057689.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(22844..23284)
                     /locus_tag="PWY67_001395"
     CDS             complement(22844..23284)
                     /locus_tag="PWY67_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2057690.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6423 a         5231 c         4092 g         7743 t
ORIGIN      
        1 tattgagcat ctgtgtcttt gaggaatacc tgttatctat tgttcggatt gctttctatc
       61 tactactttt ttacaggaaa tttacctatt agttttctct gctgtaattt ttgacttctg
      121 cttccatagc atcaataaag atattcaaat cttctacagc tgtttcttta cttccataac
      181 gacgtacgtt cactgttgca tcttccaatt ccttgtctcc aacaactaat tggtaaggga
      241 tcttttgtgt ttgagaggca cgtatcttat agcccatttt ttcattacga tcatcaactt
      301 ctacacgcaa ccccttcatt tgcaaacgtt ctttgatttc ataagcataa tcgccatgtg
      361 catcaacaga aacagggatg attgttgctt gtataggtgc caaccatgtt ggaaaagcgc
      421 ctttgtatac ttctgttaaa taagcgacaa aacgttccat tgtagaaaca attccgcggt
      481 ggataactac tggacgatgc gtattttccc cgtcttctcc aacatatgtc aaatcaaagc
      541 gctctggcaa taagaagtct aattgtatag ttgataatgt ttcttctgta ccaagagctg
      601 ttttgacttg aacatctagt tttggtccgt aaaaggctgc ctcgccttct gcttcaaagt
      661 aatctagttc taattcatcc atagcggctt tcagcattgc ttgggctttt tcccacattg
      721 cgtcatcatc aaaatattta tctgtattgt ttggatcgcg atagctcaag cggaaacgat
      781 aatctgtaat attgaagtca gcatatactg ccaccatcaa ttcaagcgta cgtttaaatt
      841 catctttgat ttgatcagga cgaacaaatg tatgaccatc attcaacgtc atttctcgta
      901 cacgttgtaa acctgacaaa gcacctgatt tttcatagcg atgcatcatt cctaattcag
      961 cgatacgaat tggtaattca cgataagaat ggatcgtgtt tttgtagacc atcatgtgat
     1021 gagggcagtt cattgggcga agaacaagca tttcgccatc tcccatatcc attggcggga
     1081 acatgtcttc ttgataatga tcccagtgtc cagatgtttt gtacaactca acatctccca
     1141 ttattggtgt atatacatgt tggtaaccta gactaacttc tttgtctacg atataacgtt
     1201 cgatcgtgcg tcggattgtt gccccttttg gaagccagaa tggcaaacca gaacctactt
     1261 ctggggaaac catgaacaag tcaagttctt ttcctagttt acgatggtct ctttcttttg
     1321 cttcttcacg gagacggata tattctttca aagctttctt gtcaaaaaag gctgtcccgt
     1381 atacacgttg catcatttga ttgtttgagt ttccacgcca ataagcacct gccactgata
     1441 ataatttaaa tacttgtata cggccagtgg atggaacatg tggtccacga cacaaatcga
     1501 tgaattcgcc ttgttgataa gcagtaatca cttcttcttc tggcaattca ctaatcagtt
     1561 ctactttgta cggatcactt gcaaaaattt ctaatgcttc ctgttttgaa agaacacggc
     1621 gttcgatcgg cagattttct ttgacgattt tcatcatttc tgcttcgatt gccggcaaat
     1681 cttctgcagt tacttggttt tgtccattat ctgtatcgta gtaaaaccct gaatcgatag
     1741 ctggtccaac gccaaagtgg atatttggat agaggcgacg catggcttgg gccatcaaat
     1801 gagctgctga atgacggact aagccaagtg catcttcatg gtctggtgtg atgatctcaa
     1861 tacttgcatc ttcctcgatt ggacgagtca aatcaacgag ttttccgttc actttaccag
     1921 ctaaagcttt ttttgctaag ctttttgaaa tgctttcagc aattgccaat gttgttgttc
     1981 cagactcaaa ttctttgact gcaccatctg gaaaagtaat ttttatcata atagtttcct
     2041 ccttgaaatg ttttgattga tcagatcatc aagctgaaaa acaaaaaaat cctagcatct
     2101 cttgaaaaga gatactagga cgaaaagttc gtggttccac ctaatgttca aacgcaaatg
     2161 cgttcctcaa gcgttgtaac ggtccgaccg cccttgcttc aacaagggtt tcgaaagtgg
     2221 tcatcctttg gttttctcct ggaaaagttt cagcctagct tttcctctct tatcgaagac
     2281 tctcaaattc agttggcttt ctcatcaatc aattatgtgt ttatttaacc actaataaat
     2341 agaaaaatca agtgggaaac taaacttatt ttttccattc cccatttaca ccgtatgttc
     2401 cttttttcat ctcattgatg atgacatgga tgtgttcttt tggtgcctgc gtgtttttat
     2461 gaacagcttc cgtgatatct ttcatcatat tttctaattg ttccggactg cgaccttcta
     2521 ctaattcgac atgtacgaat ggcataatgc tctctcctct ttctgatcgt tcttttttca
     2581 actcgattct atcatactat gtttgtcttg ggtactgaat caggaaaaat ggctggatct
     2641 ttttttgctc tcaaggcact caaaatagag accgtatcca cgacttcttg caaaacagct
     2701 ccgaataatg cggggattag tcccgtactt gcaattaaca tcaaaaaaat acaaataaag
     2761 atgccaatca gtacagactg tttcgctatt ctcatcgtat ctcttgaaat cactactgct
     2821 tttgctacac gcaagagatc atctttcaag atgaccgcat ctgcactttc actagcagct
     2881 gtagaaccat gagcccccat tgcaataccg atatctgcag cagcaagtgc tggtgcgtca
     2941 ttcacgccgt ctcctaccat cgcaacaggt ctttgatctt tagggatact ttctaaaatg
     3001 gtcagtttat cttgaggcaa gctacgggca tgtacagaat ctaattggac tttttctgca
     3061 atcgtttgag caatggcaga tccatccccg gtgatcatca tcaaattttt cacatcaagt
     3121 tttcttagct ccttgattgt ctttgctgct tctgggcgaa tctgatccga aaataggatc
     3181 gttcctgcaa aagatcgatc aattgttaca tagattgctg tattggacgt ttctatttct
     3241 tcagctgaaa caaaagaggc acgtccaatc tttacttcat gattattgac tactgcttgg
     3301 attccttgac cggtcacttc tttcagttga ctaacaggat acagttggct gtttttcacg
     3361 ttgttcacta acgaacgagc taagatatgg ctagattcct gttctgcact agctgcatac
     3421 agaagtagct cttctttttt tattccattg actggctgaa tcgcctctac ttccaatatc
     3481 cctttggtaa gcgttcctgt tttatcaaaa gcaatcgttt ttgtacgtgc gagcttttca
     3541 atcgttgttc cagtttttac aacgatcccg tttttacttg aacggctcat tcctgcaact
     3601 aaagcaatgg gcgcagctag aatcagtgga caaggtgatg ccactaccag tacctcagca
     3661 aatcgtaaag ggtcttttga gaaccaccaa gaaataccac cgatcaaata agcaatcaac
     3721 gtgaaaggga ctgcataacg atcagctaaa cggacaaatc gggcaggctt ttcttttgat
     3781 tcttcgacta gtttaataat catttgatac tggctgtctg cggcacgttt ttcagcgatc
     3841 atttggatcg atccgtctcc atttaaagac cctgacataa tctgatctcc aggtttcttg
     3901 gtttccgggc gggactcacc tgtcaaagaa gattcatcga ccgaagattc tcctttggtc
     3961 actcgaccat ctgcgggtac aagttcgcca ggcttgacta gtaactggtc atcaatattt
     4021 atttcatcga ttactatatc ctgcaatttt ccttctttga atcgatgggc aattcttggg
     4081 gtgttatcta acaaagactg cagttcttta cccgctcgtt tattcgcgta atcctctaga
     4141 ctatctccgc ccgtaagcat gatcaatatc atcaaactag cccagtattc tttgacggct
     4201 aatgttgcaa ggattgcggt aatcgctaat acatctacgc catatcttcc tgaacgtaac
     4261 gtcttgatca tttccaaaaa catcaagaag gcgagaatcc ctccagtaaa tgcgacaatc
     4321 gcaaatgcaa gggccggctg gtgaaaaaca aattcactta ataaagctat cccaccaata
     4381 agcaaagtaa gtaaaaattt tttgaaatgt gtcattgttt tttctcctct tttatgtgac
     4441 ttagagaggt tagtaataaa tttccagctg cctctttcat cttaagcata gtccttgact
     4501 agcacttttt aaaacaatag gccttaattg aaaacaacta tcactatcag cgtttccaaa
     4561 attcatcagt tctacaaaca aaaatattca gcaaggatta ctttttcgat tcatcagtta
     4621 aaaagtacct tttttcgaaa aactatgcta tacttttaat tcgttattta cggcggaagg
     4681 agcaaacatg cgatgaatga gaaacaacta gaacaaacgc acgtggatga aacgatcgaa
     4741 ctaatccaga tcgaacaaaa tatgctggat acaaaacaac aagaattgac tcaggaaatg
     4801 aatcattcga caaaagacac agctaatcac aagattcgag ctggttccaa tgaagctttt
     4861 tatgaatctg ctgttgaata tcgccaacat gagcaggaat tgcttttgaa ataccatact
     4921 cttgaagcac agcaaaaacg cctgcaaacg ttgtctgtca tgaaagatag tccttatttt
     4981 gcccgtatcg actttaaaga agaccgtgaa acagaaacat tatatctagg tatcgcttct
     5041 ttaagagatc tggcagaaga aacaatcgtt attgactggc gcgcaccaat tgccaatctt
     5101 tattatgaag gtgagattgg tcccgcattt tatgaaactg aaacagatag aatagatgtc
     5161 gatctgcagt tgaaacgaca atttaaaatc caagaaggac gtattttatc tatggtagat
     5221 acgtcggaag taatcaatga tgactttttg ctagagatat tggatgaatc atcaagcaac
     5281 catatgaaaa acatcgtctc tacgatacaa aaagcacaaa atgcaatcat tcgggacaca
     5341 acaagtaaag taatgctgat tgaaggaatc gccggaagtg gaaagacatc cgctttgctt
     5401 caacgtattg cttttctgtt gtatcataat cgcaagtggc tcgaggctga aaacgttctt
     5461 ctattttcac ccaaccattt gttttctgat tacatctcga ctgtattgcc ttcattaggt
     5521 gaaagcggcg tgcctacaca gacattcaaa aattatttgg tgcagctgct gccagagttt
     5581 tctttactag atgaacagca acaagaaagt ggttttttgc taggggaaaa agatccgata
     5641 caagtgatga aaagcggatt gaccttagtc gatcagattg atcgctatat ccaaggcatc
     5701 acaaactacg gtcccttgtt ccgtgatatg aaaataaatg gccgtacgat cctatcaaag
     5761 gaatcgattc gccaatggta taaagaaaca aatgaacaat tacctttaca tcatagactt
     5821 tctctattac aaacgaagct cctgaaaaaa ttaggcggtc tgcaaaaaga cgaaacacgt
     5881 cagcaatggg tcaaagatct ggcggaagaa cagctccaag aactatacgc aacagatcca
     5941 aatcttgaat atacagaaca aaaggagcgt tcattacgta aaaaactggc caaccaaatc
     6001 gtacgtaaaa gattccgtcg aatccatcga gggatcatga attatcaatt tgtcaatata
     6061 acgaaacaat atctccactt tctacaaact gtgccacttt ctcttctaac acgacataaa
     6121 atcagcgaga ccaggtggca ggaacatttg acaactgttc gtacacactt gcgtcaaaga
     6181 gaattgcgtc aagaggatgc tgtactattc tttttactga tgcgccgaac atatccagtg
     6241 acggttacac acaaagctcg ctatatcttt attgatgaaa tgcaggattt cgcaccatca
     6301 caagtggcat tgctgcaagc cttgttccca aaagcaaatt tgactttctg tggagatcta
     6361 aatcaaaatg tctttggaaa tgaaacgatc gttggatcat tggaagacct tttcgtggac
     6421 cgtgaagtca ctcgtttcca attaacgaca agctatcgct ccacaaaaga aatcactgat
     6481 tttgctaatc attttattgt gaaagaacat caagtagaaa caacagcaag aaaaggagac
     6541 cttcctttgc tcatccaagg aacaacagat gatatcaaat tgaattggtt agcagatacc
     6601 attcgaacga ctcagggaaa agcccggtat tggcgtacag caatcatagc taaaacacaa
     6661 gaagaatgcg aagctcttta tgctcaactg ccagacgagt tgaaacaaca agtccagctg
     6721 attttaaatg agagtgactt tatgaagcgt tccattattt tacttccagc ctttttagca
     6781 aaaggtctgg agtttgaccg agttttcttg tgggatgtag atgcagaaca tttttcttct
     6841 cagcaagaca aattgatcct ttacaccatg tgtacacgtg cgatgcatga attgaccctt
     6901 tttacctcca ataatgaaag tcctttgtta aacaatatgg atatttcatc ttatgagaaa
     6961 aaagtactgt aagtaaaaag acaactgcta tcattaatgt tagcagttgt ctttttattt
     7021 acaataataa ttgcagaaaa agtaattcaa tcatttaagg tcatttattc tatatataaa
     7081 tccaaatctg tttcaggata ttttaatttg acaagcgaaa taatcatttt cagttcttcc
     7141 atgttttctg gatattctgg gtcaaatacc ttgctatgac ggccaatccc gccattgtcg
     7201 atcaatcggt aaattcgatt ccattcaatg aattggtcaa ttgattggaa attgctgatt
     7261 ggatagccgc cacaatccaa aatctctgat acacgaaatg ctaggtatgc actatcgatc
     7321 actttatttt ttttcgtgtc tatcttctca tttctttcga taagaatttt ttcaggacga
     7381 tgtttcaagc tccactctaa tagtagaaag aaagcctcca tccgatactc ttcttcaaat
     7441 aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa tcagataatt ttgtctataa
     7501 atcagcattg gtgcgcggtc caccatacac tctaaataac ggttcagata ccctgtaccg
     7561 acatagtttc ctaatcgata gccttcaacg ccgaatcttg cgtatttttg cgctaatttt
     7621 gaacaatcag ttacgttcac agctaactct tctactttgc ggtaaccgct taagatacgt
     7681 tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc ccgtccatct tccacatact
     7741 tcatatcatt tcacatgata cttatgctct catcatacca caggttgaat tagaaaagaa
     7801 aataaatcac acaaaaaagc aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa
     7861 gactgaatac ttttcgttaa aactggttgc ttagtcttct aatgccacat tatggaacac
     7921 atgctgtaca tcttctagat cttccagtgc atcaatcatt ttttcaaatg tttcttgatc
     7981 ttctggagat agtgttacct cgttttgagg gatcatttgg atctcagcga ttgaaaaatc
     8041 agtgattcct ttttctttta atgcttcttg tatactatgg aaatccgcta cttcaccgta
     8101 aacgatgatt tgtccttctt cttccattac atcgcgaaca tcgatgtctt tttccattag
     8161 gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa
     8221 catgtaagaa accgctccgg aaacacccat gttcccgcca tttttcccaa atgctgcccg
     8281 tacgtctgct gctgttcgat tcacattgtt cgtcaatgta tcaacaatca ccattgagcc
     8341 gcttggtcca aatccttcat agcgcaattc agagtatgtt tcatctcctg caccttttgc
     8401 tttttcgatt gctcggtcaa taatatgttt tggtacatta taagttttag cgcgatcaat
     8461 gacgaaacgt agtttttggt ttgcatgcgg atcaggatca cctgattttg ctgccgcgta
     8521 gatttcaatc ccaaattttg cataaatccg gctattatta gcatctttag cagttttctt
     8581 tgctacgata tttgcccatt tacgtcccat attttcactt cgctttctga tttgatagtt
     8641 tcataacgca aaaacgtcct gttccattat acttgtgaat cacgattctg tgaagctttt
     8701 tttctttctt ccttcattct tctcattttt agtcaattat ctatactcgg attgggattt
     8761 gcccgaaatt ctgttccgga atattgcctc ccaattgata gatttgatca cttctaacta
     8821 acagttccgc ttcttgtgcc tctttttttc cgattcggga ataaatagaa atctcttttt
     8881 tttcttttaa gaatcttctt ccagcatcgg taaatccaag tagtcgaaga taatttttat
     8941 tttgttggac aggaatctct tgctttttga tattcagcaa gacataacaa gataatcgtt
     9001 ggatccgtgt ccatgtatat ctttttgttt ttacctttga cacaaaatcc tcaaaactat
     9061 cagcagttgc agctgctgtt ttcatgcgcg attctattcc ttcggtcatc tgatagatat
     9121 ctgtcagttc cagcaaagta gaagataaca accgataacg caaatacggc caataagatt
     9181 cccaagtctg cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc acaagtccag
     9241 ctattctttt tccttcttct aacgcatggc gaatggctgt tgcactggct atcctctgat
     9301 ctgtgatgat tttttcatga tagcctgcac ttttacgtcg gatcggatgt aattccatag
     9361 ggtggacata cttggcattt tcctttgcat aacttaatcc caagatatga tttggcgaag
     9421 aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat
     9481 aactatacgt ttgatcagac agttcttgaa atgcctcttc tatcctatct tgattctctt
     9541 ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca
     9601 aactgtcaca gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa tagtcagctg
     9661 actgaaccgc ccattcgacg ggaagctcca ctactaaatc cacaccattt tgtaaagctt
     9721 cattcgcgcg atgccattta tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc
     9781 tcattacagc aatgacgacc tctgctcctg ttatttttcg tgccatttcc acatgatagc
     9841 gatgtccatt gtgaaaagga ttgtactcta cgataatccc gcagcttttc atgcgcatcc
     9901 tccttcatag ttatcgataa aaaagaaata gagacaagta aaaaaaggca aaaatcggaa
     9961 aaaatacccg aactatataa actattctcg caaacacaat acttctgcga cgaaaagctg
    10021 cactgcaaga gcaatgccta tatcgttcgg atatataatt tatgttgaag atttattctt
    10081 ttcttgttaa ctcgagcagg caaaaccgct agtctttgta tgctacaaag aaccagcgct
    10141 ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc
    10201 cgctgttttc cagcattctt aaataacttt ccaatggata ggtacgctct tgatgtagtt
    10261 catcttcacg aataaatcga ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat
    10321 gttcgatgct gtgtttctcg cttcctggat aactatccca caaaaaagca aaattatccc
    10381 cttgataatg atagctatat tctggaaaaa cctcatctac ttgataggtg gagtgtacgt
    10441 caaataaaaa tcggccattt tcttctaacg cttgatagac ttcatcaaac acttgctgta
    10501 cctcttgtct atttttcata tagcacagcg aatcagaaaa acaggtaatt gcctgatatt
    10561 gtccgatttc tgataaatcg agcatattgc cttccacaaa ttggatcgat gcgtcttctt
    10621 catatgctcg ctggctggca atcatcaaca tctcttctga aaggtctaac cctgtcactt
    10681 tatatccatc ttttgcaaag gcaaccgcta atgctccagt accgcatgcc agttccaaaa
    10741 tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg
    10801 tttcgtccat cacttcatcg taaatgaaag caaatgtttc ataagccata tgttaatcca
    10861 accattcact aagatcgact agcggagcat ctgaccatag tttttccaga ttatagaaat
    10921 ttcgttctgg cgcatggaaa acatgtacga tcacatctcc aagatcgatc aatacccatt
    10981 ttccgccttc ttttccttca atacgtttta cttcatattt gttttcttct tctttatcaa
    11041 tgatttcttc tgtaattgcg ttgatttgac gttcgctatt tgctgaacag atcatgaaat
    11101 aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg
    11161 aatctgctgc ttcaatcgca atttttaatt gttctttact atctatgatg atttcctcct
    11221 aatatgagca gcttattttt ttgctaccca gtaattatat gtttcgattg tttttggata
    11281 aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa atgctacagc
    11341 ttcgtctaga tcagcccatg caatggctcg tgcatcttgt accccaggaa aatctcgacc
    11401 tggttcaata tagtctgcta cataaatgat tttgtcaagg agactcatct tagcagcccc
    11461 agtcgtatgc acccggattg catgaagaat ctctgcatcg gtgattccta attcccgctc
    11521 tacaaacgaa gcgccaacaa gaccatgcca gattgcatta ttataattca ataactccgg
    11581 atcaaaaccg tctcttacga ttaccatttt gaactcctca tctggtcgtt cttttgcata
    11641 atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt ttttggcaag
    11701 agccacggct gtttcttcta ctccaagcac atgcttgaag cgttgttcgc tcattctcat
    11761 ctgtattttt tgcatcaggt tttctctaga ataagcagta tattcattac tatagtccat
    11821 attcatacaa cccttcattt tgaatataat caattacttt atctggaaca aggtagcgga
    11881 tagaacagcc ttctttgatc ttttgacgta ttttcgtgga actgatgtcg atctcaggta
    11941 catctaccca aattacagga tatggagtat ccgttgtgta tcctggtcga cgtataccca
    12001 caaagttgac catagaagtc agctcatcga ttttatacca ttttggcaaa tattctacca
    12061 tatccccacc gataataaag taataatctg tatccggatt gttttgcgtc aattccttca
    12121 tagtatcata ggtgtaactt ttcccaccac gagctaattc aatcgtttca atctgcaaaa
    12181 aaggattgtc ttccaccgct aattccaaca tgttgagtcg atgttttgca tcaatcgtct
    12241 gtttctcatc aacatgaggt ggctgataag aaggcatcaa aaatacgcgg tcaagaccta
    12301 aattccggcc agcttgttca gccatcacta aatgtgcaag gtgaacggga ttgaatgttc
    12361 cgcccaagat ccctacttgc tttttcttct ctaagaattg aggcatttct tcaggaaata
    12421 attgtgaacg aacaaatgtc ttagcttgcg tattcatcat ggtcactcct tatattgctt
    12481 ttacttcttt tgatagcctt tgatactttt cttttgtgga aggtttgtat aatacaataa
    12541 cacgtccaat gatttggacg atttcgcaat ctatttcttt ttctaatact tgagctgctt
    12601 cttctgcaat ctcatctgta ttttgaagta acgatacttt gatcagttct cttttttcta
    12661 aagcttcttc aatttgaacg atcattgctg aattgatccc accttttccg atttgaaaaa
    12721 tcggctgaag atgatgcgct ttgctacgta aaaaacgctt ttgttttcct cttaaactca
    12781 taattttcct tcttccttaa atcaatgctt tgcgtttcaa tacatctaca ccctttggcg
    12841 cccagcccgc aattactact ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga
    12901 tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag ctccggaaat gcctcgactt
    12961 catcaggacg aggaggctgt aataatccac ctacatgttt ttgataaaat tcgtctgctt
    13021 tttccagttt cgttcgatga atatttacat catttgacac ataagcaata aatgaagcac
    13081 gattgccgct gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat
    13141 tcagctgata aactttaggt ttgatttctt ttaacggtgc aatgatccgc aaatcttttt
    13201 tacctaagta gtgcgccatt tgatggcgat ggataattcc cggtgtatca atcaaaaaat
    13261 gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg
    13321 tgattacatc ttgtactccc gctgtatttt tgatgatttg attgataagc gtcgattttc
    13381 cgacatttgt tactccgacg atatagacat cccgtccatc gcggtattct tcgatttttt
    13441 ctaatagttc cgtcatctca tgagatttct tagcactagt taataataca tctactggac
    13501 gaattcctgc ttcgtaagca cgttccttca tccattgtgt taatttagtt tttttcaatg
    13561 atttaggtaa tatatccact ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac
    13621 ggtgtagtcc tgggattagt gaaccattga agtcaaaaat atccaccaca ttgacgatca
    13681 atgcatcttt ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga
    13741 catcttgggt ctcattataa tggcgtaatc tgaaacaacg ctgacaatag acttccccgg
    13801 aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg
    13861 tttgtataac ggcaccacag ccgatacatt gtaattcttc tgtcattcga ttccacctcg
    13921 ccagcccata tcagggtgtt ttgctaatag ataggccata attctttttt cccaaaaacg
    13981 attaaattgt gtcttccaac tatctgttgc gataatgggt tttacaagga tgctacgtat
    14041 ccctgcacga ttggctccac gtatatcagt cataatctga tcgcctacca ttactatctc
    14101 atcatctgct aaatccaatt gtttctttgc tatgttgatc cctcttgcta aaggtttcat
    14161 tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt
    14221 attgtttgaa acaacaacaa cagggattcc tgcatttttc atttctaaaa tccaagtaag
    14281 caactcttct gttccatctg gattattcca tgcaatcaac gtattgtcta aatcggtcaa
    14341 tactgctttg atgcctagat tctttaattg ggcaggtgta atctgataga tggcttctac
    14401 catccatgtt ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa aaatgaagga
    14461 gcacataaag tactcctcaa ccccgtatta tactgcatat cagcaaaaaa aactacctct
    14521 atactcgttc gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa taaagattga
    14581 tcacagaaaa atttagaaac aaaaaacaac taatattggc ggaacctctt gtatcgtttt
    14641 tccagtaatt tatgctgatc taatcgaaca agttccgtca atctttcaat caacgctttt
    14701 ttcataatgt gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt ttcaggaata
    14761 actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag taattttcat caactcagct
    14821 gcttctgcgg cgcgactacc gtctttccac aaaatcgacg caaatccttc tggagaaagc
    14881 acagcataaa tcgtttcttc cagcatccag acttcatccg ctaaacctag agcaagggca
    14941 ccgccacttc caccttcacc aatgatgatg gcgatgatcg gtacctttaa atcagacatt
    15001 tcgagcagat ttctggcaat agcctctcct tcgccgcgct cttctgctcc tacaccgcaa
    15061 tatgcacctg ctgtattgat caaggtaatc accggtctat tgaacttttc cgcttgtttc
    15121 atcagtcgta aagcttttcg atagccttca ggatggggag aaccaaaatt tctgcggata
    15181 ttttcctgca gatcccgtcc tttttgaata ccgatcactg ttaccggttt gccatctaat
    15241 cgagcgattc caccaacaat cgcttcgtca tctgcaaata aacgatcacc atggcattcc
    15301 tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt
    15361 gctaactgaa cgacatcatg tgctgtctta tccacgatct atccatcctt tctgtgtatg
    15421 caattcaaga agcgtatgga ttttctgttt caattccatg cggggaacaa tctgatcgac
    15481 aaaaccatga gataataaga attcagcctt ttggaaatct tctggaagtt cctgcttgat
    15541 cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag ctttggggtt ctgctagaat
    15601 aatatcgcct tccattgcaa aactggcagt tactcctccg gtcgtcgggt ccgttaaaac
    15661 agttagataa aaaagacctt cattgctatg ccgtttgaca gcagcagaga tttttgccat
    15721 ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac
    15781 gactggaaga ctttcctttg ttgctcgttc gaataatctg gtgatctttt ctccgacaat
    15841 cgttcccata cttcccatga taaaattagg atccatcact ccaataatga agggaatccc
    15901 ctgaatggtt gctttaccag tgaggactgc ttcatgaagt cctgttttat cttgcatttt
    15961 tttaattttt tccgtatatt cgggaaatgc tagcggatct ttggttacaa gatccgtatc
    16021 ccattcttca aaactttttt catcaataat caaagctaga cgctgccatg caccgattcg
    16081 aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg tataaaggat
    16141 atgtttgcaa tttggacact ttgcccacat gttatcagga acagatggcg agttagcagg
    16201 tttgctctct ctattaggat tgatccgaat atatttcttt ttcttaaaca gagccatcta
    16261 tgcgcctcct atctccgttt ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc
    16321 tgtgctgtaa tcacctgcta tgactgcagg atgactaatc aaatccatct gaaattctgc
    16381 gttcgtgatg attccttctg tgataaattc acttaaggct cgctgcatct tcattaatgc
    16441 ttcaaaacgc gtatttccat ggacgattat ttttgcaatc attgaatcat aatatggcgg
    16501 gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt cccatcccac ctgctggaag
    16561 cagtaagttt tggatcttcc ctggagaagg agcaaaatgg aaagcaggat tttctgcatt
    16621 aatccggcat tcgattgcgt gtccttggaa tactacttct tcttgacaca cattcaaagg
    16681 ctcaccggct gctatctcca cctgtttttt tactaaatcg attcctgtga ccatttctgt
    16741 tactggatgt tccacttgga ttcgagtatt catttccatg aaataaaaat cgccttcttc
    16801 atccatcaga aactcgatcg ttccggcatt ttcataattg actgctttag ctgctcgaac
    16861 agcagcgtct cctagtgctt ctctttttgt ttgtgagatc actacagaag gagattcttc
    16921 cagtactttt tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg
    16981 accaaatcga tcacctaaga tctgaacttc tatatggcga gcaggataaa tgattttttc
    17041 caaatacata tcatcatttc caaatgccgc tgctgcttct tgctgagctg aagaaaaatg
    17101 ctgtgggagc tcttcttttg attgaacttt ccgaatgcct ttcccgccac cacctgcagc
    17161 tgctttcagc atcactggat aaccaattct gtctgctatt tccaatgctt cttctactgt
    17221 agataataca ccagtacttc ctggaatgac tgggacattt gcttcttgca tcaattgacg
    17281 cgcattgatc ttatttccca ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat
    17341 attgcactct tcacacatgg aggcaaatcg gctattttca gataaaaaac caaatcctgg
    17401 atgaattgct tcggcttttg tcacgattgc agcgctcagt acttcctgaa cattcagata
    17461 tgaatcaacg gcttttgccg ggccaatgca gattgcttca tctgctaact gtgtatgaag
    17521 tgcttcggca tctgcttctg aatatatcgc tacggtttgg atccctagtt cgcggcatgc
    17581 acgaatgata cgtacagcga tttcaccgcg gttagctatc aatactttag aaaacatatt
    17641 tcacctatcc aatcataaag gttaattccg cttcggctac ttttttaccg tctactttag
    17701 cgattccttt gcctattccg gctgaagctt tcactttcaa tatctcaact tcaaggtata
    17761 aggtatctcc cggtgtaact ttcttacgga attttgcttt gtccaatccg ccgaaataag
    17821 ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct tgagccattg
    17881 cttcaacaat caatacccct ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg
    17941 gctcattgat cgttacattt ttcttagcaa cgatccgttc tccttcaacc atttcttcca
    18001 ctcgatcaat caaaagcatt ggataacgat gtggaatgat ttcttttatt tcttgtatgt
    18061 tcattgtttt ccctctttcc tgctttgctt atttttgctg aatcctaaag agtggctgtc
    18121 catattcgac aactgactcg ttttctacaa ggatttctgt gatcactccg tcggcctcac
    18181 tagttatctc attcatcagt ttcatcgctt caataatgca gataacttct cctttggcaa
    18241 cttgatcacc tacttttttg aaagctggtt tgtcgggcgc tggttgcaga taaacgattc
    18301 ccacgattgg cgaaacaacc tcgattccat caaatactgc cgattctttt tctttcatta
    18361 tttctggagc agttgttgct tgttcaggta tgacattgac tggctccgaa actggcaacg
    18421 tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt atttttattc atatacaatt
    18481 caaattgacc ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat tgactgacta
    18541 attctctgat ttcattgata tccaaaatta ttcctcccag cgtttcatgc acagaaccgc
    18601 attatgtccg ccaaacccta atgaattact cattgcataa gtaaaacttg caggtttgct
    18661 ttcgtttttg acaactgtca agtcaatctc aggatcttgt ttttctacat tgatcgtagg
    18721 tgggataaat tgatgttcta atgccagcaa agttgccaag gcttcgatcc cgccggctgc
    18781 tcccaataaa tgtccagtca tcgatttcgt gctgctgacg taagtatctc tgaaattttc
    18841 acctaatgcg atttggattg cttttgcttc tgcctggtca ttggctggtg tacttgttcc
    18901 gtgagcattg atatatccga cttgttccgg ctgtacacca gcttcttcca tcgcaagctt
    18961 cattgcttta gatgcaccag aaccatctgg agtaggagct gtcatatggt aagcgtcaca
    19021 gtttgcaccg tatccaacga tttctccata gattttggca ccgcgagctt gtgcatgttc
    19081 taaagattca agaactagaa cgcctgctcc ttcacccatc acaaaaccat tccgattttc
    19141 gtcaaacgga atcgaaccac gattaggatc agttgattct gagagtgctg ttaaagctgc
    19201 aaatccagca atccccatct cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc
    19261 agaatagcca tgtttgatgt tacggaatgc ttccccaatt gaattcgttc cgctagcaca
    19321 agctgttacg atagctgtac aaataccttt tgcacctaca cgcaaagcaa tatttccagc
    19381 tgccatgttg gcaatcgcca ttggtacgaa cattggagaa actcgttttg cacctttttc
    19441 atgtagacga ataacttggt tttcgattgt tggaagacca ccgatcccag atccgatgat
    19501 gacgccaaac cggtcttgat tcattttttc tttatctaaa ccgctcatct ccattgcttc
    19561 taaagcagcg tagattccat agatcgaaaa catatccatt cgtttgctat cttttttaat
    19621 aaaatattta tcgtaaggga aatctttgac ttcacccgct aaggtaatcc ctgtttctga
    19681 agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa
    19741 gctgtctgcg tcatttccaa taggtgaagt gactccatag ccagtaatta ccactcgatt
    19801 catttgtttg ctctcctttt cttatccgtg catgaccaat ccgccatcga cattgatgac
    19861 ttgtccggtg atatatggat tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac
    19921 ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt tccttgactt tgtctgctaa
    19981 aacttccgtc atatcagttg tgatgaatcc tggcgcaatg gcattacatg taatgcctct
    20041 agttgcagct tctcgtgcca cagattttgt taatcctact acgccagctt tactagctgc
    20101 ataatttgct tgaccaatat ttccaatcaa cccagataca ctagagagat taatgatggc
    20161 cccttctcgc tgcttcatca tttttttcaa tacgtgctgc gtcatattga aggttccaat
    20221 caaattgata tccagacatt ttttgaaatc ttctgcatcc attcgcatca caagtttgtc
    20281 atttgtgatt ccggcattgt ttactaacac atggatcgat ccaagctttt cttctgcttc
    20341 tttgatcatt tgtcccgctt tctcataatc agagatgtca ccggaaactc ctacgcattt
    20401 tacaccgaat gcttcgattt cttctatctt ttcttttgga atctctccgc gaccattcaa
    20461 aatgatattt gctccagctt ttgcaaaagc taaggccatc gctttgccga ttcctcttgt
    20521 actacctgtg ataaatacat tttttccttt tacatccata acgttcctcc ttatagtatt
    20581 gctattgttt catcaaatgt ctgcttattc tcaacacgca gaactggtat tgttttatcg
    20641 atttttttca taaatccgct taatactttt ccggggccga cttcgatgac ttgttcgatg
    20701 ccgattgttt ttaacttatg gatactctca tagaaacgaa caggttgcat gacttgctgt
    20761 tcaagtagtc ccgcgattgt ttctttcttc attatttccg ttgttgtgtt gcttatgata
    20821 ggaaaagaag gttcagagaa ttgaatctga cttaggtcct tcgccagttt ttttgctgcc
    20881 ggttctaaaa tagctgtatg aaaaggcccg ctgacattta gagggatcat tcttttgaac
    20941 cctttttctt ttaatagtaa aacagcttcg tctactgctt tttcttcgcc accaatcacg
    21001 atctgctgag gtgtattata atttgcagga gatacgatgc cgtacttgct agcttcatga
    21061 cagctttcct cgatcgtttc aatcggcgca ttcatcactg caaccatttt cccgcttcca
    21121 gctggggcag cttctgtcat ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg
    21181 aaggatagcg cattactagc tactaatgcc gaatattctc ctaaactcag tcctaaagct
    21241 gcatctggga tgattccatg ctccttcaat aagcgataat acgcgatgct gactgttaga
    21301 atagctggct gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca
    21361 gccatatcgt agccgagaat ctcacttgct tcatcaaatg tctgctttac aacggcttct
    21421 tcatataaat cttttcccat accttgatat tgtgcacctt ggccactaaa taagaatgct
    21481 gttttcatgt ttttctccaa tatatccaga atatatgttt gattcttcct attcaaaaag
    21541 gccgagacat tcctatttca ggaaatgtcc cagcccccaa cctaatttta atgattatgc
    21601 agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc
    21661 gattttcacg tcaaattcat cttcaatttc attgatgatt tggaataaat ccaagctgtc
    21721 agcttctaag tcttcttgga tgtcagtagt caatttgatt tcttcagttt cttttcctaa
    21781 ttcttctgcg atgatttctt gaattttttc gaataccata ataatatatt cctccgatat
    21841 tgtttgcttt ttatttttat aatttaacag tctacaactc catgagaata cttccccatg
    21901 ttaaacctcc gccatagcct gtaaatatta ctttctgctg ttttcctaag gtgatctttc
    21961 cactagaaac tgcttcatcc aataagatag ggatgcttgc agcagatgta ttcccatatt
    22021 tatccatatt ggtcaggaac ttttcttgcg gcatctttac ttttcgggca attttctcaa
    22081 taattctgag gtttgcctga tgcaaaagca gataatcaac tggtttgtct ttcatgactt
    22141 cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg ccagtcattt
    22201 gcaaataata cgaaccttct gaatcttctg aatagaacgg atttttattt tgctggtagc
    22261 cagcggtcaa tgagctactt ctggtaccgt cagctgctaa tttttctttc agcaattttt
    22321 tctgtggatt cgcttctaag agaatcccac ctgctccatc gccaaacaac acagcagtcg
    22381 tacgatcaga ccaatcgatt agcttagata gtgtttcacc accaatcacc agtcccttag
    22441 ttgagccgca gcggatcatt ttttcagcca tgcttaacgc ataaacaaat cctgagcacg
    22501 cagcactgat atcaaaagcg atagcatgag ttgctcccaa tttcccttgt acttgtgctg
    22561 ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa tccaaatctt
    22621 tagggtttag atttttctgt tttatcaatt tttccgccac taaaatgcat aaatcagaag
    22681 tctcctgatt tgttacacat cttcgttcac gaatacccgt ccttgacgaa atccattcat
    22741 cagaggtatc catcatcttg gaaagatcat tattagtaat aatcttttca ggaaggacgc
    22801 tggctgttgc agtaatcatt ccaaattgtt ccattaaata tcctcactta tagtcttgca
    22861 agaagtcgtg caagtttttc agtgcatcca gcaaggcatg ctgttcctcc gtagccattc
    22921 cgtctaagat gttcttgacc atttcacgat gaaaatgctg atggacccga aatagtaact
    22981 ttcctttttt cgttagtccc aactttacaa cgcggcggtc atcttcactg cggatacgtt
    23041 cgacatagcc tttttttact aacttgttaa tagcagtggt caaggtccca acagtaattg
    23101 atagttcttt ggctacttct gaagttgttt ttttcttgta catcccgatt gcctcgatcg
    23161 tgtgcatctc tgtgattgac agatctttga attgagattt tttcagctca gactcttcga
    23221 ttgtcaaaat atcattgaag acactaacaa gataatcatt tactgttttt aaatctggtt
    23281 ccatgttcca ccgtccacta attagtttga ttatcaaatc ttttgatgtt caaactatat
    23341 cttaactttt ttcaaaatgc aaccttaaaa gatcattttt ttactatatg agcttttttt
    23401 aagattttac tcatttaaaa caactgataa atcagcattt ataatttctt ttattagaaa
    23461 aatcatctat ctcacatatt ttgattatc
//
LOCUS       DANKAZ010000037        23302 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000037 DANKAZ010000000
VERSION     DANKAZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..730
                     /locus_tag="PWY67_001396"
     CDS             <1..730
                     /locus_tag="PWY67_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2057691.1"
                     /translation="LFSSDQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKRL
                     VKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFI
                     LVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTIL
                     TILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQS
                     VVGFVLLMITNTIARRFDSDSALF"
     gene            742..1662
                     /locus_tag="PWY67_001397"
     CDS             742..1662
                     /locus_tag="PWY67_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2057692.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1703..3160
                     /locus_tag="PWY67_001398"
     CDS             1703..3160
                     /locus_tag="PWY67_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2057693.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3505..4119
                     /locus_tag="PWY67_001399"
     CDS             3505..4119
                     /locus_tag="PWY67_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2057694.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4112..5836
                     /locus_tag="PWY67_001400"
     CDS             4112..5836
                     /locus_tag="PWY67_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2057695.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5833..7284
                     /locus_tag="PWY67_001401"
     CDS             5833..7284
                     /locus_tag="PWY67_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2057696.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7757..9910)
                     /locus_tag="PWY67_001402"
     CDS             complement(7757..9910)
                     /locus_tag="PWY67_001402"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL2057697.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9912..10958)
                     /locus_tag="PWY67_001403"
     CDS             complement(9912..10958)
                     /locus_tag="PWY67_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2057698.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11108..12397
                     /locus_tag="PWY67_001404"
     CDS             11108..12397
                     /locus_tag="PWY67_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL2057699.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12410..15112
                     /locus_tag="PWY67_001405"
     CDS             12410..15112
                     /locus_tag="PWY67_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2057700.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15097..15954
                     /locus_tag="PWY67_001406"
     CDS             15097..15954
                     /locus_tag="PWY67_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2057701.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(16025..16924)
                     /locus_tag="PWY67_001407"
     CDS             complement(16025..16924)
                     /locus_tag="PWY67_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2057702.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            17093..19264
                     /locus_tag="PWY67_001408"
     CDS             17093..19264
                     /locus_tag="PWY67_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2057703.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19332..19673
                     /locus_tag="PWY67_001409"
     CDS             19332..19673
                     /locus_tag="PWY67_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2057704.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19949..21703)
                     /locus_tag="PWY67_001410"
     CDS             complement(19949..21703)
                     /locus_tag="PWY67_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2057705.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21714..22550)
                     /locus_tag="PWY67_001411"
     CDS             complement(21714..22550)
                     /locus_tag="PWY67_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2057706.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22552..23235)
                     /locus_tag="PWY67_001412"
     CDS             complement(22552..23235)
                     /locus_tag="PWY67_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL2057707.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT         7659 a         4166 c         4787 g         6690 t
ORIGIN      
        1 tttattttct tcggatcaag catttctcat cactaaaaat acaattttat ataatgtagc
       61 atttatcctt ttgaatctgc tgatctcagt cgtatttgca atcattatga gcgagttgag
      121 aaataaacga ctcgttaaag tttatcaaac gatgtcctta ctaccttatt ttctatcttg
      181 ggtcattatc agttactttg tttacgcttt tttaagtcct gacaaaggaa tattcaatca
      241 gtggatcact ggacatggag gagaagctgt caattggtat aatgaaccga aatattggcc
      301 gtttatcttg gtgttcattg gtacatggaa agggattggt tataacagca ttatttactt
      361 tgcatctgtt atgggaatcg atccgactta ttatgaagct gctatggttg atggagctag
      421 caaatggcag cagatcaaac atgtgacgat accacaactt attccactaa tgacgatttt
      481 aacgatttta gccgtaggaa acattttccg agctgatttt ggcctgtttt ataacattcc
      541 taggaattcc ggtgcattat atgatgtgac tcaagttttg gatacgtata tctacaatgg
      601 cctgacatcc acaggagatt ttggtatgac ggctgctgca ggactttacc aatcagttgt
      661 cggctttgtc ttgttgatga tcacaaatac gatcgcccga cgttttgata gcgattctgc
      721 attattctaa ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca agtccgctct
      781 ttcaataaga cggctaattt tttctctaat ataatgattg cactctttgc tatttcctgt
      841 attttgccat ttatatttgt tatcgccatc tcttttaccg atgagtccat attgtcgact
      901 cacgggtaca gtttttggcc agaaaagttc agtatatttg gttatacgta cttgttcgac
      961 cagatgcagg ataaaatttt ccaagcgctt ttcgtcactg tacttgttac tgttttaggt
     1021 acactgatca atagcacggc tacttcttta tatgcttatg cgatttcacg gtcaaacttt
     1081 ccattccgtc gtttttttac cgttttttgt ttgattacga tgttgttctc accagggatg
     1141 gtggccaatt atcttgttat gacaaatctt cttcagttaa aagatacgat ttgggcattg
     1201 atcttgccaa tggctgtcag tcccttcaat attattgtga tgcgcacatt ctttaaacgt
     1261 tcggtctctg attcgattat tgaatcggca cggatcgatg gagctagcga actgcgaata
     1321 tttgttcaga tcgttttacc gttagctgtt ccaggtattg cgacaatcag tttatttgcc
     1381 gcattgggtt actggaatga ctggttcaat gctttactat acatccaaga tgataagcta
     1441 gttccactac agtatctttt gatgaaaatc caaagtaata ttcaatatct gacacaaaat
     1501 tcaggagcag gaagccaatt gacaggcgga ttagcctcag ttccaggaga atctgccaga
     1561 atggcgatcg tagtcatctc gacgttgcct attgcgatca gctatccatt tttccagaag
     1621 cattttgtca aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa aaatataaat
     1681 cataaaatta ggaggaagaa aaatgaagtt atggaagaaa ctagccttta caggtgtttc
     1741 agcgttgttg ttgggaacat tagcagcatg cggaggttct ggttctaaag accaggcaga
     1801 agctagcaat tcggacactt tgcagatgta tcaaatcgga gataaaccag ataatttcga
     1861 tcaattgatg gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa aagtcaatat
     1921 caattacatt ggctggggcg attacgaaaa gaaaatgaac gtcatcattt cttccgggga
     1981 aaattatgat atcgcctttg cgaacaacta tgtttccaat gcccaaaaag gagcctttgc
     2041 tgatttgact gaattagcgc ctaagtacgc tgaaaaagct tataatgatt tagatgaagc
     2101 atatattaaa ggaaatctgg tcaatggaaa attatatgct ttcccagtca atggaaatgt
     2161 ctttgctcaa caagtactta cttttaacaa agctttacta gataaatacg atctttcaat
     2221 tgatggaatc caatcttacg ctgatgcaga aaaagtcttg aaggaattcc atgaaaaaga
     2281 accaaacacg gcagcttttg ccattggaca aggcttcaaa gtccaaggga actttgatta
     2341 tcctttaggg aatacgttac catttgctat tgatctggat ggagatgatt cgaaaatcat
     2401 caaccaatac gataatgaaa cgtatatcgg actgttacgt acaatgcata gctggtacaa
     2461 acaaggactc attccaagtg acgctgcaac gagcaataag gattatccac tagaaggaaa
     2521 tacttggcta atgcgtggag aaacacaagg accgtatgat tatggcgata caattttgac
     2581 aaatgcagca aaacaagaac ttgtctcaaa agcaattact gttccactaa aatctacagc
     2641 tcaagcacaa atggcaaatt ttgtcgtatc aaatacttca aaaaataaag aaaaatcagt
     2701 agaacttcta gggctgctta acagtgatcc agaattattg aatgggcttg tttgggggat
     2761 cgagggagaa gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat tgctagatgg
     2821 ttatcagcca aatacacata tgtctgcttg gaatacagga aacaacaaga ttctatatac
     2881 acaagagtct attacggatg acatgattgc caaacgtgat caatcgattg ctgatgcaga
     2941 aacttcaccg attttaggat tcagcttcaa cactgacagt gtcaaaacag aattaagtaa
     3001 tatttctaat gtcatgaatc aatatttaga tggattgaat acagggactg ttgatccaga
     3061 tgaaacatta ccgaaattga aagacgcttt ggacaaggct ggctatgata aggtattgaa
     3121 agaaatgcaa aaacaatatg atgagttccg acaagaataa taatgaataa agacaaaggg
     3181 gttgtgacaa aagacgtgtc acaacctctt gttccgaata aacggtgttc aaaagccagt
     3241 ttctcggtat taagctaaat atatgtaaaa atgtggaaag ccatattcac aaaatttttc
     3301 ttactactcg aaactaagcg gctatttcac aaactcttgg taaacggtgt tcaatcagct
     3361 aaccctcggt attcgctcac aaaacgacaa aatatgagga actatttttc cgtttgtttc
     3421 tctaataatc aggtcaaggt gctgattgcc caaccttttt ttgtataatg agaaagtata
     3481 aacttaatgt ttggaggaga agatatgtta gggaaggttt tggaagagtt atttatccgg
     3541 atctgggtca ttataaaact aaccttgtat ttctggatat atacatttgc cggcgggatc
     3601 atctttggat taggtgcggc atggaaaaca gtcaatgaac tattttattt gtacggattc
     3661 gagtataaag aaatcacaat aaaaagaggc tggaatatat ataaaagaaa ttttttaaga
     3721 ggcaatctgc tatttagttt atttttatct ggtactgctt tattgagtta caatttatat
     3781 ttatctgttc aaatcaaagg gctcattttt ttagctattg attttatttt actttttgcc
     3841 ttgctctgcc tatttgctac ttatcaatac agtatgattt tagatagtga atatacgatc
     3901 tctgttccta atctgctaaa gctaagtttt atttcggttt tttccagctt ttcgtctttt
     3961 ctaaaaataa ttattggtag cggtataatt ttaggcgtga cttggcaatt caaaggattg
     4021 atcctgtttg gtgtcattgg tcttttgacg gtttggaatg gtacgatgac aacacattgg
     4081 cgtgaagaat tagataaaca gttggaaagt tatgaataag tttcggattc tattttcaaa
     4141 aggaacctta ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg gtgtaacgat
     4201 gactgctacc gttattttta gtatccatac ttgggaacaa aatcaaaaac aggcagaaaa
     4261 catgacaagt gatgccgttc aaagcacaag cagaatgctg aatgataaaa caaccttgag
     4321 taggatcatc aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg tgacgaccta
     4381 cttaacaaaa cccatagatc agtatctgat gtatgtatac gaacagcaaa acagtaccga
     4441 tgaattagtt tctttcccaa atcaaatcaa agatctctac gcaaattatg aagaactaag
     4501 tgcgatatat atcgtgttga atcaactacc ggagtattat gaatcttcta gagaaaacaa
     4561 aggtggggaa ataaaaaagg ggacaccaaa attactagac gccttttata tcaagttgcc
     4621 tatcacacaa ggaggagctg agtcaggaag tatttttatt ggttttcaaa agaaagaact
     4681 agatatgatt ttagaaaatc taacgtcatt caatggactc tctttataca tgatttctgg
     4741 aacgacaaat cgactataca cattccatga taataaaata gctaaagaaa cgtttgaaag
     4801 acaagaaatg atcatcacag agagtctgaa gaaaaattca caattgccga ttgacgatct
     4861 ggaaaaaaac aattttgttc aacaccaaga actgtctgat gattatcgta tattagctgt
     4921 actagataaa aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc tgatcattgg
     4981 tatcattatt ttagacaata tactgcttat ctttctttat aaaacattca aacgatattc
     5041 acagcaagta tctatcgtca tgcaggccat ggatcaaaca gcagcaggga atttagagac
     5101 gcggatcgac ataacaaaca cggaatatga actgagggaa ctttcaatcg gtatcaacga
     5161 aatgttggat agcatcaatc agtttgtaga agacatctac aaattagaaa tcaaacagca
     5221 agacgcacat atgcgggcgc ttcaagcaca gatcaatcca cactttttat ataatacatt
     5281 agaatatatc cgaatgtatg caatcagcga aggaagtgaa gaattagcag atgttgtgta
     5341 tgcgttttcc gctttgcttc gaaacaatac gaatcaagag aaaacgatca ctttaaaaga
     5401 agaactggac ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc ctaatcgagt
     5461 agcctatcat tttatgatcg atcctgattt ggaaaagatc gaagtaccta aatttgtgat
     5521 ccagccatta gttgaaaatt attttaaaca cggtattgat tttactcgat tcgataatgc
     5581 gctaagtgtc aaagtcttgc aggaaggcaa acgggtaagg atcatcatca aagataatgg
     5641 aaaagggatg actgaaaaac ggctaaaaca gattgaagaa aaactgtctc atcctaaagt
     5701 ggaacttcac ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg ccagcttcgg
     5761 cagcagttat tatatgtcgc ttgagaataa tgagacaggt ggactgaccg tttctattac
     5821 attcaaggag ggatgagcgt gtatcaagtg ttattggtag atgatgaata catgattttg
     5881 aatgggttaa aaaagataat tgattggcaa agtttaggct ttcaaatagt agcaacagca
     5941 gagaatgcca aagaaggact agctgtactt gaacagcgac agattgattt agtagtaacg
     6001 gatgtgacaa tgccagaaat aaatggactg gaatttattg aagcggctca aaaagaacgt
     6061 cataattttg aatttatgat tttatcagga taccaagaat tcgattattt gaaaggaggt
     6121 atgcaattag gcgccgtgaa ttatttgatg aaacccgtga ataagttcga attggttgaa
     6181 agcctcaaga aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa tcaacaagaa
     6241 atctatcaag aactgttatt cagtcaatgg ttgaatgaag aattggatga aataagtgaa
     6301 gaagaaataa taggacaaat aagtgagaag cagcatagga tagtattgat tcaactggca
     6361 agaactcatg atactcttat caatagatgg ttgcaagaac ggcaacaaat cttttactat
     6421 caacgaaatt acggagaccg tgtattgatc acgttgttgt tggaggcaga tgaagttgat
     6481 ggcttctgtt gttttgttca agaaaacttt cctgatcagg aatggctgat cagtatcggg
     6541 gaagagacag attctttgga caaaatccct gaaacattcc aacaagcgaa agacaatcta
     6601 cagttgcatc aattttatgg agataaagac gagcatgttt tttacgcagt acaagcagaa
     6661 acaaaagatc aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa taaacaattg
     6721 gaagtcgctc aaaaaatgat cactgatttt tttgaacagt ttcagttagc agccatgatg
     6781 ccggaagata tacgatattc cagtttttta ctgtttatgg agattcaacg ggaattgatt
     6841 gatctggaag atgaggaata tttacaagga atcgaaaaaa tccatcaggc aaaaacggtg
     6901 caagaacttc agcagctact tctgtcattc atcaaaaagc atcagagaca gaaaaagtac
     6961 agtgacaatg tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga accacttaca
     7021 ctcaaagaag tgtcagaaaa cctgcattta aatgtcatgt atctaggaca gctgttcaaa
     7081 aaagaaacga aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga aaaagcgaaa
     7141 caactgcttt tgcacagtaa tcaaaacatc aatgagatag caagtgagat tggctacaac
     7201 aacaccacgt atttctctaa attattcaag aaaatcgtcg gcagatcccc taaagagtac
     7261 cgagaaaaca gtggagaatc ataaaagaaa aagccaatgt aatgaaataa cttataaaaa
     7321 attgtctaga aatctaattt tgggctgttc ttattacttg aggcaaggca gctttttcat
     7381 gacctctcga taaacggagt tcaaaagcca gtttctcggt attaagccaa atttatgcaa
     7441 aaatgtggaa agccattttc acaaaatttt tcttaatact cgaaactaaa cagctttttc
     7501 acgacctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaatgg
     7561 caaaatatga ggaactattt tgccatttgt ctctctaata ctcaggtcag aacgctgatt
     7621 tcacaacctc tcaataaacg gtgttcaaaa gcttgcatct gcggtaataa gaccaaccaa
     7681 acgaaaaata tgaggatcta tttttgcttg gctgttctta ttacttgatg caggacagct
     7741 ttttcacaac ctcttgttaa ttatagtttg ataaggaaaa aggcaaatct tttgcttgat
     7801 atttcttagg gtgagggatg atccctaatt ggtaagagat cgtgccgcct ttcagcaaat
     7861 cttgatgggt aaaatataat ttcgtatatt ctctgtccat ccgtttgatt ttatggataa
     7921 attgttgctg cggccgatta ggcgttgctt caatcgtcaa ttcctttcca tttgacaaaa
     7981 gcaaaacagc cttgtcaatt aatggcatac cgataacata ttcaccggtt ccaggcgtga
     8041 caggataaaa tcccagacta ttgaagatat accaagcggc catacttcca ttgtcctcat
     8101 ctccagggta acccattgga gaatcgtcga aggtctgagt catcaactgt ttaagcagtg
     8161 gctgtgccat ttcaggttgt cctatataac tgaacaagta aggatagtga aaacttggct
     8221 ggtttgaaat agctaattgt ccaaattcta tcgcggccat ctcactcatt tcgtggatct
     8281 caaaaccata tcctcctggg tcaaacgttg ggggctgatt gcacagctca atgagttttt
     8341 ctttatatct ctcactgcca ccatattttc cgatcagtcc tgaaaaatcg tgataaacag
     8401 caaagctgct ttgccaagca cttccttctg cataatcttg tccccaacga ttagctgaaa
     8461 atggttctct gaacttgcca tctttatctt ttgaacgcat aaatcctgac tcatagtcaa
     8521 agatattttg atagttcaat gattgctgct gatatttctt cgtcatttcc tcttcttcta
     8581 gtaccttggc aacttggcta atacaaaaat cgctataagc atagtctaat gtgtgattga
     8641 ctgattcgtg gtagtgatta ggaacatagc catatgtcaa gtaatcttct gttccttggc
     8701 gtccataatt tggatgtttg ctttgaaccg tagcaccttt ttccattgcc gttagaaatt
     8761 caggcatcaa gtctcgtcgg atatctttta cagcagcatc tgcaatgacc gcatcgatca
     8821 atgtcccagg cataagtcca cgttcatcag gagacagcca ttttggcaaa tagccacttt
     8881 ctctatagct gttcaaaaaa ccttccagca tttcttcgta tttttctaca gcaatcaaag
     8941 aataaagagg ataaaccgtt ttatacgtat cccaaaaccc attatttgta tagagaacac
     9001 caggttttat agatcgacta gtcgtatcat agtggatttt ttgcccacta ggatcaattt
     9061 cataaaatgt ttgtggaaat aaaaacaccc gatataaatt atgataaaac gtccgcaatt
     9121 gttgttgatc atggtgtgtg agctgtatac gatcaaaata ggactgccat tgttcttgac
     9181 tgtgagcaag gtaagtctct ttgctttggt tttgttctag ttccaggtta taagctgctt
     9241 gttccgagct gataaaagat gtcccaaatc ggatagtttg tttttttgtc tgttcaaaat
     9301 acaacgggat cgagtcgcta tttttgttgg aaacagctaa ctcatcaatt gttaaaggat
     9361 attcaaatct caatataaaa taaaaagaaa aatcaggatc ttctgaacca gcaaaatgtg
     9421 tgatttttcc ttccactgtg taaggatcta taacaaagag ctgatggcgt cccggaaagg
     9481 ataaaagtaa accgctgtca tttttgctgt aatcaataga taagattccg ccatacatgc
     9541 taggtatcaa tgtagaacag atgccataac gtaattgaca gatcgacaga tgagtcgggc
     9601 aaaatatact ttcttctggg cggtaagaac tttgagcatg gaaaagggtg ttctcctgta
     9661 gttttccatt gatcggcgta aacagcatat gactgaaatc acccatccaa ggacttggtt
     9721 gatgggtaag acggtagcct tgaaaaacgc ggtcatcagg atgaaaccac cagctgccgt
     9781 tttgatccgt agtttgcggt gcaaaataat tcatcccaaa aggaacgcca gtataaggct
     9841 ggcagttccc attagagaaa tttggctgat tcgctgttgc atgtctagta tctatttgac
     9901 cgatattcat tttatttctc ctttgttgat tgtcttttga tgaatgaaac tggaaattta
     9961 ctatcggatg gtcgatcttt tgtctctaac caagtgacca aagcatctcc cgcaagctta
    10021 cctaactgtt caaaatcttg tgcgatggtt gtcagtggcg ggtcaacaag agctgctgct
    10081 tggatatcat caaatccgat aattgaaaaa tcttcaggga tatgataacc atgctgtctc
    10141 aagagattca tggtctggat agcgacaaca tcgttttcac aaacaaaagc agttacttca
    10201 tacttagtga tataagaaag caattcatct aatgtcgcgc ttttgtcatc cagtgaagtg
    10261 tggaaagtca aaccagcttt gtccaatgct tcaaggtaac caaggtaacg ctgtcgaacg
    10321 gattgtggat gagtctgcgt accaaacaaa taagcgattt ttgaatgtcc ctgttcgatc
    10381 aaataagagg cagcaagcgc accgccttgg aaattatcgg aaagaacggt aggaaaaggt
    10441 aactcataga tttttttatc taatatgacg acagggaatt ctttcaatga aagctcaaaa
    10501 agtaagtcaa gatgttgttc tgtatcaaga gcataataaa tcaatccgtc aaattcattg
    10561 attagctctt ttgctgtttt attttgtaga aagtcaagcg tcgtcatcat gacatcaatc
    10621 tgtttatctt gcatcacagg gttcaatccc ttgctaaaat caccaacaga caaatcagcg
    10681 acatatggaa gaagaaacag aatccgtcgt gtttttgtag gagaagcaag aatctgttcc
    10741 ggagaagcgg atttaacgaa actgcctttc ccgcgaacgc gataaataag tcctaattgc
    10801 tctaattcag tcaatgcacg tttggaagtg attctgctta ctttataggt acgtgaaagt
    10861 tctttttcag ttggtagttg gctgtctaca ggtaattcac cagttaaaat agccgtttgc
    10921 aaatcattaa atattgtctg gtacaatggt tgagtcatat aaacacctct taatgatata
    10981 tcatattcaa ataataccat aatggatgaa aaagtaaata aaaagatatt ttttgaaagc
    11041 gtagacataa ttggctgtat gaagtataat ctttaaagat atatcaaata gaaagaaggt
    11101 tttattagtg gtttataaag atataccaaa atcggtgcag aaatttatgg aagatatcac
    11161 acaaaaatgt ggggatatcc atgcggactg ggcaaagaat tttacagcag catttgccaa
    11221 tactctgttg acaacagtca aaaagcaaaa tgacggaacc acctttttat tgacgggaga
    11281 cattccagcg atgtggttaa gagactctac tgcacaagtt cgtccttatt tagtgattgc
    11341 aaaagaagat gaggaattag ctgacatgat cagcgggtta gtcaaaaagc aattttttta
    11401 tatcaatatc gatccatatg cgaatgcctt caacgaagaa gctaacggtg ctggccacca
    11461 aacagatcat acaaaaatga acgattggat ctgggaaaga aaatatgaaa ttgattcttt
    11521 atgctaccca atacagttgg cctatcttct ttacaaaaat acaggaaaaa ctgatcaatt
    11581 caatcaatct tttattgaag gtatcaaaaa aatcttggac gtgttcaaaa cagagcaaga
    11641 tcatgaacac tctccttata catttgtacg agacacgaca agaaaagaag atacgctgat
    11701 caatgacggg cgaggtgcaa aaacagcttt tacaggaatg acatggtctg gtttccgtcc
    11761 tagcgatgat gcttgccgtt atggatatct ggtaccatcc aatatgtttg cggtggtcgt
    11821 gttaggttat gtggaagaaa tttttcaaac gatactcgat gataaagaaa ttgtacagca
    11881 aacacagaaa ttgaagaaag agatccaaga ggggattcag aatcacggac gaacggttaa
    11941 tcaaaaaggg caaacgattt atgcatatga agtagatgga atgggaaacg ccacagtcat
    12001 ggatgacagt aatgtgccca atctgatcgc cgcaccttac ttgggatatt gttctgaaca
    12061 agaggaagat tatctgcaaa caagaagaac attgttgagt aaagaaaatc catatttcta
    12121 tgaaggaaag tacgcaaaag gaattggatc ctcccatacg ccagaaaaat acgtttggcc
    12181 gattgcatta gctatggaag gaatgacaac gtcagacaaa tctgaaaaag agcggatatt
    12241 gaatttactt gtagcaactg acgcaggaac acaccttatg cacgaaggat tcgatgtgaa
    12301 caatcctgaa aattatacga gagaatggtt ttcttgggca aatatgatgt tttgcgaatt
    12361 agtcatggat tactttgata ttagagtaga aaagtaggag gtatcgtaaa tgaaaaagaa
    12421 agtatatatc atttctcata gccactggga tcgtgaatgg tacatggctt acgaacagca
    12481 tcacatgcga ctgattgaat tgatagacga tctactagag ctatttgaag ttgatccgtc
    12541 cttcaacagt tttcatttgg atgggcaaac gattatttta gatgactatc ttcaagtaag
    12601 gccagaaaaa agacaggcaa tccaacaagc aatcaatgaa ggaaaattga gaattggacc
    12661 attttatatc ttgcaagatg attttcttat cagctcggaa tcgaacgtga gaaatatgct
    12721 gattggcatg gaagaaagcc gtaaatgggg aacccctgtc atgttaggct attttcccga
    12781 tacatttgga aatatgggac agacgcctca attaatgaaa caagctggta tttcagcagc
    12841 agcgtttggc cgtggtgtaa aaccaattgg ctttgataat caagttttgg aagcagaaaa
    12901 ctattcttcc caatactcag agatgtggtg gaaaggccct gatcaaacag caatctttgg
    12961 tctgctattt gccaattggt atagcaacgg gaatgagatt ccagtagaaa aagaagcagc
    13021 tcttgccttt tggaagcaaa aattagcgga tgcagaacaa tatgcctcta cgaaccactt
    13081 attgatgatg aatggtgttg atcatcaacc ggtacaaaaa gatatatcaa aagcaatcca
    13141 tctcgcaaat gaactgtttc cagattacga gtttattcat tctaatttta ctgactatct
    13201 ggaagcagtt caaaaagatg tgccagaaga cttaggctca gtagaaggag aattgacaag
    13261 tcaagaaacg gatggctggt atactttagc caatacagca tctgctcggg tttacttaaa
    13321 acaatggaat accaaggtcc agcgacagct agaaaatatt acagaaccac ttgcgacgat
    13381 ggcttatgaa gttagtggaa actatcccca tgatcaatta gattatgctt ggaagacatt
    13441 aatgcaaaat catcctcacg acagtatttg cggttgttca gtcgattctg tccatcggga
    13501 aatgatacct cgttttgaaa aagcagatga agttggaaag tatcttgcac aagattcctt
    13561 agaacagctg accgcagcaa tcgatacgac cggattccca aaagacagct ttccatttgt
    13621 cattgtaaat actgcaggaa tggataaaac tggtgaagca gaaataacca tcgaattaga
    13681 aagaaaaaga ttcgctgaag gaatacctga acaactatat caagagttag aaaatttgcc
    13741 taaacgtaag tatcatgtag aaacaaaaag tggggccact ataccagcta ttttatctga
    13801 agaaactgtc caatttggtt atgatttgcc gaaagaccgt ttccgtgtgc catatatggc
    13861 aagaatgatc aaagtgactt taccattaga aaacatgcca gcattttctt gggaaacatt
    13921 cgcgcttgta gaaggggaag cgaaagcaga agaaaaggaa acaatgattc atcagagtgg
    13981 acgaatcatc gaaaacggcc ctctacattt gacgattgaa aaaaatggta ccattacaat
    14041 ggaagatcgc aaaaacaaac gaaagttgaa taatctgcat atttttgaag atatcggcga
    14101 tataggaaat gagtatatct ttaaacaacc tttctgtgat cagccaatcc tatccagtaa
    14161 caaagaaaac tctgaagtaa aagtattagt agatacaccg gaaatagcaa aaataagtat
    14221 tctacaggaa atggaaatcc ctgtttctgc agacgagcgt ttagaaaaag aacagcagat
    14281 ggttgtggaa tttcgttatc gaaaagcaga acgatccaaa gaaaaacgaa tccttcaaat
    14341 aaaaacaatc atgacaatca gaaaagatag taaaaaaatc gactttgaaa cgactctcga
    14401 caatcagatg aaagatcatc gcttacgtgt cttgtttcct acaaagttac atgtggaaca
    14461 tcacgaagca gatagtattt ttgaagtggt gaagcgccca aaccatgtgt ctaaaagttg
    14521 ggaaaacccc acgaatccgc aacatcagca agcatttgtc aatattcatg atgaagagta
    14581 cggtgtaaca gtcggaaact ttggtttgaa tgaatatgaa gtaacagaag acggacaaat
    14641 cgcagtgacg ctacttcgca gtgtgggaga actaggcgat tggggatatt ttccaacacc
    14701 agaagcacag tgtctaggtg agcatcggtt caattacagc attgaactac atggaccaga
    14761 agaaaaattc tctacttatt tgcatgcata cgcagcacag attccttttt ccactcaaca
    14821 aatcaaacat catgaaggaa cattgatttc aaaacaacaa tatttaacga taaaaagtga
    14881 aacgtttgca attacagctt tgaaacgcag caagtttagt gataaagtag tagtaagagg
    14941 tttcaatatg tctagtcatt tagaaaaatt ggaaataact aaagacaatg gaaaaactgt
    15001 catattgaat ctgcttgaag aaccaacgaa gcaagcggtt gtcccaataa tccaacctta
    15061 tgagattcgg acaatcggat tcgaggagga aaacgaatgt atggcggaat agaagcaggc
    15121 ggaacaaaat ttgtgtgtgc ggtttcagat ggtggagaaa ttttagaaaa attaagtatc
    15181 cctaccacaa caccagaaga aacattggat caggtatttg aattttttga tcgttatgaa
    15241 ttgaagggaa tcgggattgg ctcattcggc ccgattggta tcgacaaaac aaaagataat
    15301 tacggatacg ttttggcaac tcccaaaaaa gggtgggcac aattcgattt tcttggcaca
    15361 atcaaggaac gctacaatat tcctgctgct tggacaacgg atgtcaatgc tgctgcatat
    15421 ggggaattga agcaaggagc agcacaagga aaaaatagtt gtatttacct taccgtggga
    15481 acaggaatcg gtgcaggtgt cgtgatcaat gaagaaattt tttcagggat cgcacatcct
    15541 gaaatgggac atatttgggt gaaacgccat ccagatgacc attatgaagg tacttgtcct
    15601 tatcataaag attgtttaga aggattagca gcaggaccgt ctattgaggc acgcacaggg
    15661 atcaaagggc aagatctgcc gcaagatcat cctgtttggg ctattcaggc ttattatatt
    15721 gctcaagcac tgatcaacta tacgttgacg ttagcacctg aaaagattat tttaggcggt
    15781 ggggtaatga atcaggatca tctccttcaa aaaatccgtc agcaatttgt tgaattgatg
    15841 ggtggttata tggaaacacc tcaagtaagt gaatatatcg ttcgttgggg actgccgaat
    15901 gaaagtggga ttatcggtag tttgctgtta gcagaacacg aacatcaaaa gtaagcagac
    15961 aaattacaca aaaagaacag agtcttatca ttatctgctg ataagactct gttctttttt
    16021 atttctattc ccataatcca ggtaaagctt taccgactat tttttcttga gtaaatggat
    16081 ggatcatgtg cagttctaat gcatggagca tcagtcgttc agctgttcta ttttggtaga
    16141 gagggtctcc aacgatcgga tgtcccttat gagctaaatg aactctgatc tggtgtgtac
    16201 gtccggtttc caaaacacaa tagacttgtg tttgttcaga aagtactttg ttgacagtga
    16261 catgtgtcaa ggcgctctga ccatttttat catctacgac cctttttctc cgatcatggc
    16321 gatctcgtcc aattttattg gtgatggtca tatctttggc aatttctccc catacgatgg
    16381 cttgatatct ccgatagatc attttttgtt ccaacattct tccaagtatc ggcaagacaa
    16441 atggattttt tgcaaataaa acggcaccgc ttgtttcttt gtccaagcgg tggacaacgt
    16501 aaggtcgttg ctgtttagtg tcgagataag cagctaaatg attgagtaag gtactgtttt
    16561 ctgtcggttc atttggatga gtcttcattc ctgcaggctt atccactata atcaaatgct
    16621 catcttcata taaaacacgg atgtgcttcg catctcctaa ttgaacttct tgataattat
    16681 aatctgtttc ttcgaataac aatgtgatct ggtcattgcc ctttacttca tgatggaaca
    16741 aagcaggttg ttgattgatc caaacgtttt tacgtatacg caaaaagtgt cgaacctttc
    16801 taggaactaa ccattccttt tcaagcaagt cgcgaattgt tgtttcagga tggttcatag
    16861 gtaaagtgat cgtgattttc acaagtgtac tcctttcagt gcaattgcag actaattgta
    16921 ccatttttaa aacatattta agaaaaaact tacatttttc tgttcattct tatgttactc
    16981 tacttgcact aacgtaaaaa aacatattga actacaactt aagcaggaag aaaacactct
    17041 ttcactatgg taaaataaga cgattgaatc aaatagacag gagaagaagc aaatggattt
    17101 tcaaagcatt ttagaaaaga taaaagctgc tttgatcagt ttttggcgtt ggatcagacc
    17161 atatctcgga caattccatc gatggcgaaa gcgcatctgg aaaaaatatc atgtgaacaa
    17221 attgattttg ctgattggtt tgatctgtgt cttagtgaca agtatctacc tctttatttt
    17281 agcaaaacaa gcaaatgtcg aaacgttaaa atcaggatta agccaatcga cagtcattta
    17341 tgataagaaa aacgaagaag ctggcacact gtacgcccaa aaaggaacat atgtagaatt
    17401 aaacgcgatt tctccctata tccaagatgc agtaatctcg acagaagata ggaattttta
    17461 cgagcatcac gggtttgata tcaaagggat tgcccgtgca gcagtccgaa tggtcttgtc
    17521 tggcggaaca tcagggggcg gaggaagtac gatcactcag cagcttgcca aaaatgcgta
    17581 tctgacatta gaccaaacgt ttgatcgaaa agcgaaggaa ttatttttgg caatcgagat
    17641 cgaaaagaag tacagcaaag aagaaatctt gaccatgtat cttaacaatg cctattttgg
    17701 caacggggta tggggtgtcc aagacgcttc cagaaagtat tttggagtag atgcaacgaa
    17761 tgttacgctg tctgaggctg ctactttggc aggaatgctg aaaggtcctg gtatttataa
    17821 tccgatcgat cacccaaaaa atgcgaatga tcggaaaaac acagtactaa gtgtaatgga
    17881 agaaaacgga aaaatcacaa aagaacaaga acaagcagaa agccagacag atatcagcgc
    17941 gtatcttaat gacacatacg ataattcaga ttctggttat cgttatcctt attattttga
    18001 tgcagtgatc gatgaagcag ttaacgagta tggtcttgat gaagaagatg tcatgaataa
    18061 aggctataaa atctatacag ctttagatca ggattatcaa aagcaattag aagctactta
    18121 tcaaaacagt gcattattcc cagcaaatgc ttcagatggt gccatggtcc aatctgcttc
    18181 agtagcttta gatccgaata caggtggtgt acaagcacta gtaggacgaa gaggagatca
    18241 tgtctttaga ggattcagct ttgctacaca gatgcgtcgt tcaccaggat caacgatcaa
    18301 accattaagc gtatatacac cagcactgga agctggatac aagccttcca gtatcttgaa
    18361 agacgaaccg caaagttatt acgatgcgca taattttgac gggacatacc aaggagaagt
    18421 accgatgtat caagctgttg cacaaagttt gaatttgcct gctgtatggc ttttgcatga
    18481 gatcggttta caaaaaggat atgataaagc agaagaattc ggcctgcctt tagcaaaatc
    18541 tgataagtat tatggcttag cccttggtgg actggaaaaa ggagtatctc cattgatcat
    18601 ggcgggtgct tacagtgcat ttgccaatga tggtcagatg tatacgccgc atcttatcac
    18661 gaaaatcgtt gattcgactg gagcggtgat cgtcgataac acaaacaaaa aaccaaaaca
    18721 ggtcatatca aaggaaacag ccgatgaaat gaccagcatg cttcttggta cattttcaaa
    18781 tggaacagcg gctgctgcga atcctgcagg ttatacgata gcagggaaaa caggaaccac
    18841 agaaactaat ttcgatgcca caaaagcaaa tgaccaatgg atgatcggtt acacgccaga
    18901 cgtagtgatt tcgacatgga tgggttacga agaatccagc aagctgcact atttggaagg
    18961 gacaagtgga aatgttgtcg ggaaagtctt caaatcagcg gcagaaggca tccttccgta
    19021 tacttcaaaa acaggattta gtgtagcaga tgcctatgcc acaggaggac aagtggtagc
    19081 tgcagatcaa gttggaaaca caggaaattc taatggagag tctggaaatt ggcaagataa
    19141 cttgaatcaa tatggtgaac aagcaaaaga agggctgaaa aacttcggcg atatggtgaa
    19201 agaaggcgtt cagggaattg gtgaagcggc taaagacctc tggcggaaat atcaaggaga
    19261 gtagcccctt tgaaaacgaa taaccccatg ttacaataga gagtaactac taaaagaaaa
    19321 gagggcataa catgtcaaac atctacgata gcgcgaatca aatcgaacgt gagatccgtg
    19381 aattaccaga attcaaagcg ttgaaagaag cgtacgagaa agtacaagca aacgaagaag
    19441 cacacaaact gttcaaagac ttccaagcga tgcaaatcga attacaagaa aaacaaatga
    19501 gtggacaaga gttcagcgac gaagatgcaa tgaaagcaca agaaatggct gtgaaaatcc
    19561 aatcagaaga agtaatcaat gacttgatgc aaaaagagca aggcttcagt acaatcatca
    19621 atgacttgaa ccgtatcatc atgacaccag ttcgcgacct ttacagcgaa taaaactatt
    19681 ttatgaaaca acagagggtg tgaaagagac gttcggcctt gagcgatgac aaaattgctt
    19741 cttattgccg ataggctgtc tcttgaacac catttaccaa gaggtcgtga gcctaccgtt
    19801 taactctgag ttacaaggaa caatttcaaa aaacagcttc tcatgtttga tgactattgt
    19861 gacttgtgac cgaggagttg gcaggagaac accgtttatt cagaaatagg agctacgaca
    19921 aaagtcttgt catagctcct attttttgtc attcattttt caagcggtag aaatagcctt
    19981 tttgagctaa cagtttttcg taagtaccta tttcaacgat ttttccttca tcgatcacaa
    20041 cgattttatc tgcctctttg atcgttgaaa gccgatgagc cacaataaaa gtagtgggtg
    20101 tttctgtcag gtaattcagg gcttgctgga tttttttctc tgactggtta tctaaagctg
    20161 aggttgcttc atctagcaaa atgatttttg gctgacgtat caacgctcgt gcaatggaga
    20221 tacattgctt ttggccacca gacagcttgt tgccatgttc gcctactaag gtatccagac
    20281 catcaggaag ctggtcaatc agtgaagaaa gttgcgcagc ttcgatgact tcatctaaac
    20341 gttcttttga tacgtttttc aagccataag tgatgttctc tttgatcgaa gcggagaaca
    20401 acaaagtggt ttgaggaaca acggctaaat aatttcgcac acttcgcata tccagttcat
    20461 ccaaaggctg atcatctaga aaaatttttc ctgaagtcgg ctggataaat ccaatcaata
    20521 agttaaggag tgtagatttt ccagagccag atggcccaac aaaagcaatt gtttctccag
    20581 ctttcacctc tagagaaaaa tgatcgataa tcggtttgag tgagtccgga taaagaaaag
    20641 aaacatcctc aaaacgaaaa tttccatcca aatgttggat ttttttctta ccgtgataag
    20701 tttctacatc gccagcaatc aacagttcgc caatagaagt gatagattcc atgccttttg
    20761 aaatcgtagg aatcagcgtg atcattccag aaacggaatt gacgatcgtc gtgaaatacg
    20821 tctgatacat cacgacatcg ccgggttgga tgcgcccttg taaagctaat atcccagtga
    20881 aaatcaaaca aatcagctga aatatctgaa aactagccca gctgactgat ccgaaaacag
    20941 attgcaggat atctaagtgg aatccttttt catagatttg agtaaaatgc ttatttaagc
    21001 gagttgtttc gtgtttttct aacccatgag ctcgagcaat cggtgctaat tcgatcactt
    21061 cgatcacttt tgcagaagtc tcttccattt cttgcctgaa gtctgagttg gactgtctga
    21121 tccttttacg gaaaaagaga gtaacgaatg cggcgagagg aacagtcaat aagaaaaaca
    21181 tgaaaacaac aggactttta taaagcgtga tacctaaagc tacgaataaa ttgacagcga
    21241 tatttaatcc gctgacaaat acttgagagg acaacgtttg gatagcttct acatcacgga
    21301 tgatctttga ctggattcgt ccagattgca attctttttg ataaggaata gatagttctt
    21361 ggattttctt gataagggtc gctcttagtc cagcttctac ttgacggatc gaagtgctat
    21421 ggtattttac atgaaaataa ttcgtcaata tattttgacc aatcagacaa gccatcaaaa
    21481 aagcgttgaa ataaatggcc tgcaactgag aagaatcttt cgatgaagct gcattgatta
    21541 cattggcaat agcgatcggc attaaccaag atggaagatg tttgataata aaaaagagtc
    21601 ctgacatggc aaaacgccaa taatttccct gataaagatg cagcaatgtc tgaaaagcat
    21661 ttgcatgata tttttgataa cttttctgta ttcgttcctc cataatcttc tccttaacaa
    21721 tcttggtaaa taaactgcaa gtgttctatt gcttcaggaa cataaatctc aggagtagct
    21781 tcaagagtgg cgtacattag aggacgctca tatttgatat aatgcagtac cgggcggaaa
    21841 tctaaacagc ccttgccaac aggtaccatg cggatctttc cattttccat gatatagtct
    21901 ttgagatgaa gcgctgtaat ataaccatgt aaattgtcca aggcacctcg gatcactttt
    21961 tcctgatcag catgattttc tttatgcatc aaattcgcac aatctaaaat gatttttaaa
    22021 tttggtgaag ctacttcatc gattaaacgt ttagctaact gatagttata gattggatga
    22081 ttgatccctg cttcaacagc aacagttacg ccaaaatttt ctgcatgagc gactaattca
    22141 atgatagaat ttctggcgat ctgataagct tcttcagtaa aattttcttt tgtgtaaccg
    22201 acagagacac taccggtttc cgtagcaatc atcttggctt gataggcaga gcacagagca
    22261 atttgctgtt tgaaagttgc caatgcttgt gcacgaactt cttgattttt gctggatagg
    22321 ttgatatagc atcctaaaat ggaaagttcg atattgtttt tttggaagta ttcaccaaaa
    22381 taggcagcac tcccaagtgt catgtcagtt aatttaagtt gttcaggaaa tgctttttgt
    22441 ggtgcaaatt ggatatggga caagtgaagc tgtgatactt tttgaacgat ttcttcacga
    22501 tttcgaacat ctaaatcgtg ggcacggatg cctaaattta atggaaacat ctcaaactcc
    22561 tttttgtaaa taattagcaa gaggcagacg ggattctttt atcaattcaa caattattcg
    22621 ggcaacttta tcagcgcctt ctggactaaa gtgggtattg tcttttagtc cttctgggta
    22681 atttttacaa gtatcaggca taagatgcag gtagaatttt tttgtctctt cttttgagaa
    22741 ggtctggaac aattcttgtg atcttgagaa cacatctaaa agaggaatat ggtgtttctc
    22801 agcaaatgca cgcatcgctt ttggatagtc tccaagtata tctggattca atgttccgtt
    22861 ttctaagtaa tctcgtcgcg tcacagatgt cagtaagacc atttggacgt tcgctttttt
    22921 tgcgcattca aaaaatatct gcagattttt taaatagcta ccgaagggaa gcgtacctct
    22981 ttcttcttgg attttctggt cgttatgtcc aaattgtaca aataagaaat cattcggctg
    23041 aaactgtgaa gctagacggg ctaaccttcc ttcctggata aatgatttgg tgctgcgtcc
    23101 attctttgct tggttattga tctggatctc gggtgaaaaa taagcagaga ttttttctcc
    23161 ccaacctgtc tcgggacgct tgttttcatc tttttctgcc gcagtagaat caccggcaat
    23221 atggatagta ggcatgttat ctcttctttc ataaaggttt ataggcaatg aagcagaaca
    23281 gcaagagaag aagacggagc aa
//
LOCUS       DANKAZ010000038        21988 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000038 DANKAZ010000000
VERSION     DANKAZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..395
                     /locus_tag="PWY67_001413"
     CDS             <1..395
                     /locus_tag="PWY67_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057708.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="PWY67_001414"
     CDS             398..1147
                     /locus_tag="PWY67_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2057709.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="PWY67_001415"
     CDS             1159..1428
                     /locus_tag="PWY67_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057710.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="PWY67_001416"
     CDS             1433..1597
                     /locus_tag="PWY67_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057711.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="PWY67_001417"
     CDS             1590..1892
                     /locus_tag="PWY67_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057712.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="PWY67_001418"
     CDS             1889..2050
                     /locus_tag="PWY67_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2057713.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="PWY67_001419"
     CDS             2047..2352
                     /locus_tag="PWY67_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057714.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="PWY67_001420"
     CDS             2352..2708
                     /locus_tag="PWY67_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL2057715.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="PWY67_001421"
     CDS             2695..2913
                     /locus_tag="PWY67_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057716.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="PWY67_001422"
     CDS             2910..3329
                     /locus_tag="PWY67_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDL2057717.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="PWY67_001423"
     CDS             3326..3883
                     /locus_tag="PWY67_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL2057718.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="PWY67_001424"
     CDS             3880..4176
                     /locus_tag="PWY67_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057719.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="PWY67_001425"
     CDS             4253..4666
                     /locus_tag="PWY67_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2057720.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="PWY67_001426"
     CDS             5012..5386
                     /locus_tag="PWY67_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057721.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="PWY67_001427"
     CDS             5423..5590
                     /locus_tag="PWY67_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057722.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="PWY67_001428"
     CDS             5616..5960
                     /locus_tag="PWY67_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2057723.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="PWY67_001429"
     CDS             5965..6246
                     /locus_tag="PWY67_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057724.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="PWY67_001430"
     CDS             6349..6663
                     /locus_tag="PWY67_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL2057725.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="PWY67_001431"
     CDS             6641..8335
                     /locus_tag="PWY67_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2057726.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="PWY67_001432"
     CDS             8355..9533
                     /locus_tag="PWY67_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2057727.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="PWY67_001433"
     CDS             9496..10182
                     /locus_tag="PWY67_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2057728.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="PWY67_001434"
     CDS             10182..11342
                     /locus_tag="PWY67_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2057729.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="PWY67_001435"
     CDS             11352..12227
                     /locus_tag="PWY67_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057730.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="PWY67_001436"
     CDS             12224..12535
                     /locus_tag="PWY67_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057731.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="PWY67_001437"
     CDS             12525..12878
                     /locus_tag="PWY67_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2057732.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="PWY67_001438"
     CDS             12868..13269
                     /locus_tag="PWY67_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057733.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="PWY67_001439"
     CDS             13262..13666
                     /locus_tag="PWY67_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057734.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="PWY67_001440"
     CDS             13678..14286
                     /locus_tag="PWY67_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2057735.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="PWY67_001441"
     CDS             14306..14668
                     /locus_tag="PWY67_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057736.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="PWY67_001442"
     CDS             14671..14853
                     /locus_tag="PWY67_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057737.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="PWY67_001443"
     CDS             14870..18289
                     /locus_tag="PWY67_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2057738.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="PWY67_001444"
     CDS             18340..19077
                     /locus_tag="PWY67_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2057739.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="PWY67_001445"
     CDS             19087..21378
                     /locus_tag="PWY67_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2057740.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="PWY67_001446"
     CDS             21402..>21988
                     /locus_tag="PWY67_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057741.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DANKAZ010000039        21971 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000039 DANKAZ010000000
VERSION     DANKAZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(6..1316)
                     /locus_tag="PWY67_001447"
     CDS             complement(6..1316)
                     /locus_tag="PWY67_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2057742.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1328..2746)
                     /locus_tag="PWY67_001448"
     CDS             complement(1328..2746)
                     /locus_tag="PWY67_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2057743.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(2986..4554)
                     /locus_tag="PWY67_001449"
     CDS             complement(2986..4554)
                     /locus_tag="PWY67_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2057744.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4558..4839)
                     /locus_tag="PWY67_001450"
     CDS             complement(4558..4839)
                     /locus_tag="PWY67_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL2057745.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4836..5759)
                     /locus_tag="PWY67_001451"
     CDS             complement(4836..5759)
                     /locus_tag="PWY67_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL2057746.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5756..6634)
                     /locus_tag="PWY67_001452"
     CDS             complement(5756..6634)
                     /locus_tag="PWY67_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL2057747.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6621..7958)
                     /locus_tag="PWY67_001453"
     CDS             complement(6621..7958)
                     /locus_tag="PWY67_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2057748.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(7961..9064)
                     /locus_tag="PWY67_001454"
     CDS             complement(7961..9064)
                     /locus_tag="PWY67_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2057749.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9138..10646)
                     /locus_tag="PWY67_001455"
     CDS             complement(9138..10646)
                     /locus_tag="PWY67_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2057750.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10612..11304)
                     /locus_tag="PWY67_001456"
     CDS             complement(10612..11304)
                     /locus_tag="PWY67_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2057751.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11326..12300)
                     /locus_tag="PWY67_001457"
     CDS             complement(11326..12300)
                     /locus_tag="PWY67_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2057752.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12600..13472
                     /locus_tag="PWY67_001458"
     CDS             12600..13472
                     /locus_tag="PWY67_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2057753.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14054..16252)
                     /locus_tag="PWY67_001459"
     CDS             complement(14054..16252)
                     /locus_tag="PWY67_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2057754.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16484..17476
                     /locus_tag="PWY67_001460"
     CDS             16484..17476
                     /locus_tag="PWY67_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2057755.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17536..18957
                     /locus_tag="PWY67_001461"
     CDS             17536..18957
                     /locus_tag="PWY67_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL2057756.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            18950..20449
                     /locus_tag="PWY67_001462"
     CDS             18950..20449
                     /locus_tag="PWY67_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL2057757.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20488..21882
                     /gene="uxaC"
                     /locus_tag="PWY67_001463"
     CDS             20488..21882
                     /gene="uxaC"
                     /locus_tag="PWY67_001463"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2057758.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6643 a         4488 c         3818 g         7022 t
ORIGIN      
        1 taatttcaac gattgtcaaa agtattgttt gcagctgtgt tgccagtttc tatagataaa
       61 tcacagcttt tgaaacataa gttttgtact gcttcgattc gcccgaattc ttttgcagtt
      121 atttggcaat gagaaaagca gacatcttct ataggctttt caggaaatgc tactaaatca
      181 aaagctcggg aaggaagtga gtaacctgga gaaaggcatg cctgtacgtt tttgatgtgt
      241 atgtctttga ctttcgtcat ttgctttgat tcaggtattt cttcagccac tgcttgccaa
      301 ctttctggca tttgagcaag gtcattgcta ttttttctat tgtattcatt atgccagtcc
      361 attatccaag aaaaaggaaa ttgtacatca atcatattca gattttctac cctgatattt
      421 tcgatcactc cccctctttc ttttgaagat ttcattcgaa taccacagtc tgactgaaaa
      481 aaattgatat cgtgaatata tacgtcacta actccagcac taacttcgct accaatagtt
      541 acaccgtatc ctgaacggat tttacatcta gcaacctcga ttcttgatga ttttcgattg
      601 actcttgcac cattgccatc tctgccagac ttaatagcaa tacaatcatc accacaggcc
      661 aaatcacatt catagacacg cacatttgtt gaagaatcaa tatcaatccc atctgtactt
      721 ggtccatcat tgttgcggat gattactttt tctactaata catcgttaga ataagtgatt
      781 tgcaagttcc aaaaacctga tttctgaaaa atcaaatcac gaacaacaac atgttctgca
      841 tgatacagta aacaagcgcg tggacgttta atagcataat ccgcaatcca acgaagtccc
      901 ttttgatcgt attcagctct tgtcccgctt ttttgatctt ttccccaata cagctcccac
      961 caatggtctc cttgaccgtc gattattcct tttccttcta taagaatatc tttcgccgag
     1021 agaacattca aaatcgctgc tggccatttc atttctacgc cggctactcg tgtatcgatt
     1081 tctggaaatg cttcgatatc tgttctcccc ttgaggactg caccttcttc aaagataaga
     1141 tgactcttat cttttaaaaa aagggctccg actaaataaa tgccttttgg aataaataca
     1201 ggtaagtgtt ctttcgcacc ttgatcgatt gctttttgca gtattattgt atcatcttgt
     1261 tgagtgtcgt ttgttttaaa gttcaaagca ttgataatcc ctttttttgt ccgcaatacg
     1321 tttctcccta tctttcattg atttttttca attcttcacc aataaataca gtttttgaac
     1381 gattcagtgg gtaacctact agcaaaatga ttgcggatac aaataagcag aatgctggta
     1441 acaagtttgc catcgtataa atcctttctt gtactaaaac tgattggatg gctccacctg
     1501 ttgtagacga ttggtaacca atgagtgcca gcataaatcc gcctaccgct cctgcacatg
     1561 cttgtcctac ttttctcgca aaagaattta ctccatatac tgttccatct tctcttaagc
     1621 ctgtcttata ttgatgataa tcaatcacgt cagtaatgaa tgcccagacc atcaaattga
     1681 ataaaccaga tcctaaagta gcgataaata aaagaatcag atatacccat gcatttgtta
     1741 tatgaaggaa atacatcagc aagtacatac atgaagaagc aagtaacgca aatacgcttg
     1801 cttccttttt accaaataat tttgtcagct ttgtagcaaa tggtgctaaa actactactg
     1861 ttccgtaagt aaataaaaga gcaacagaca ttgcttgttt attatggaag taatcattga
     1921 ataaataagt catattcgtc ccagctaaaa tctgattgat cacaatgaaa atgtcaacaa
     1981 tgactaaaat aatcagtgct cgattagcta acaaaccttt taccaactga gaagcagata
     2041 ctttttcgtt tttgacaact cttactcttt cagtagtcaa tttacaagtt aacatatatg
     2101 cgacaaatgc tattgcagca catccgattg aaatccaaaa gaaatgattc cctgaaacga
     2161 cttgttgtcc actcttgtta tgggtataca ttaaaatcgg aatgaagaat cctgtagttg
     2221 atccgccaat cgcagaacca atgcttcgga acgtggacaa cgaggtgcgg tcattaggat
     2281 ttgagctaat tgctgctgcc attgctccat acgggatatt gattgttgag agaaaaatcc
     2341 cataaacaat gtatgaaaca aaaatataag caatcttcac cgtcattgga agatgttgga
     2401 cgaatggcaa aaacaaaatg acagtcacta agcaaaatgg atacttcatt tttctgatcc
     2461 aaggaataaa tcggccagtt tctgttaact ctgagacatc acataatctt ccaaccatca
     2521 catcagcaaa cgcatccaaa attctcgaaa tcaagaataa cgtacctacg attgcccctg
     2581 aaacgccaag aacattcgta taataaatca tcaagaaact attgactaaa cctaaaataa
     2641 aacagttacc tagatcaccg aacatgtagc ctatcttatc ggaaatccca aattcctttt
     2701 tttctttttt tactgtattt gctgtaacag cattttgtga aagcatttga attcctccta
     2761 cctcttctag taaacgtttt caaaattata actctttttt tcttgttttt atattcacaa
     2821 tgttggcttt ttattgcatt atcttgacag ataaaaaaca aaaaataatc taaaaacatt
     2881 gatgcataag attgataact ttttttacat aacaatatat acaaacaaaa aaattcacta
     2941 actagtgaaa ataagttagt gaacctcatt tattaaagaa ttaaattaca gatgatcttt
     3001 tccatcatac ttgaaaaact caaaatcagc ataacttcct ctttttttat ttagatcatg
     3061 cactgctatc ccgataaaat ttccagtaaa ccctcctgag agtatacaca tgtttttttg
     3121 cggacctact ttcttccatt cttcagttga tacgtctttc aaatgaaact gtgctgtctc
     3181 tttctttaca gtgactttca aaaaagtctt cttttctgtg agtggaatcg tccaatcttc
     3241 tatttgtgct actccgtttt ctgattctat catacgtagg cattttccct tgtcctcatt
     3301 ccaagtaaca taacaataaa tataattgga ttcatttaag tacagtacca gtccagccat
     3361 ctgattaaaa ttattaggca tgtattcaaa tgctgtctcc gcatcaaaag taaaatccgt
     3421 ttgccgaata gctaacaaat gatgttcgaa ggtagattga agagattcac cggaaatcag
     3481 acgtaaaaaa cctggtctac attttgtatc acaccaatca gcatttggca aaatgcgtct
     3541 ggcattccag ttcttcttta gctctccttc aaatgtatcc ataaactgat gatctttttc
     3601 ttgttgtacc tcttcatttg tctcaatcat tatttcttcc atcggagttg tctggctatc
     3661 tttaagtctt aaccaatcgt cggacgtcca atacacttct tgaatagcgg tttctcttcc
     3721 caaaatagcc gcaccgttta atggtctggt acaaagatat gtcatgtacc atcttcctga
     3781 cggggtttgt actaagctcc catgtcctgt acattgcaat gtagaagcag gtttgtcact
     3841 agctgtcagc ataggaaaac caggatcaag ctcgtaagga ccagtaatat tctttgctcg
     3901 gcaaactgta actgaatgtc ctcttcctgt accgccttct gccgtcatca aataataata
     3961 cccgttacga aaatacaaat gtggtgcctc cgttttcgct aattctgtac ctgcaaatat
     4021 tttatagatg ggaccatcaa gcttcttctt ctctgcatca tattcttgta atacgatacc
     4081 gctggattta ttcggtgtgt ccagacgata atcccatagt tcattgacaa gccatttttt
     4141 tcccgtttca agatcatgga acaaagaagg atcaaagccg ttactattta aataaattgg
     4201 ttcggaccaa ggacctgtga tctcagatgc agtgatcaaa taattgtgga catctttaaa
     4261 cggacggcct gcacttttga catctgtgta aacaagataa aacaatccat cagcataact
     4321 gatctgtggc gcccatatac ttccatttgt aggatttcct tgcaaagata cttgagtggt
     4381 caaaatatcc gttgtatgtg accagttgac taaatttttc gactcatata ctcggattcc
     4441 tggaagccat tcaaaagagg agacaataat gtaataccgc tctttcactc ttaatatatt
     4501 aggatctgga ttgaatcctc ttaaaatagg atttttcact ttgatagtct gcatgattca
     4561 ggctcctttt ctcattctta ctcgatataa actgaatgcc ccagctgcta aaaaagcaag
     4621 tgaaataaac aacacaacgg caaacagacc agtcatgtcc tgtaataatc caaatgtatt
     4681 ttctaaagac aaagttgaat gatctacagc aagcgactga taactttttt taaactctga
     4741 cgcagacata ttacttaaag taactgctaa cccgccaaag aaaatcaaag atagactgcc
     4801 accgaccatt cctagcaata gttcccattt tcttttcatt tctgaacccc ctgcttttct
     4861 aaatgtatcc attcaggtct tttgatcgat gtttcataat tgttattacg ttcagagaat
     4921 ttgacagtct gttcattttt cttgtcatcc caattatccc aaccagttgg gtgaacatgc
     4981 ccacctatct tacacgcttc aaataagaca tttgcatacg gacgccacgg tcttcctaaa
     5041 tagacgtttc tagtatcagg ttctgcagtc aaggtacagc tttcaaaacg ataccctgtt
     5101 ttttgatttt tcggcgtaga tgctgctgtg atgtatccag gatttttact ggctaagcta
     5161 tggatatgac actgatgaaa aacggcatct gccccaccaa atatgaaatc caccgttcct
     5221 tcaatccagc aatattcata ggtctgacga caatactcag gattggcaga agccggtgta
     5281 caaaaaggag taccatcttt ttgcaaatca ggcaacggac ccgtgcataa ggtatcctga
     5341 taaccagata gtcgacagtt ttttaaagat acgcgatctc ctgaattgaa taaagcaaca
     5401 gcctgtccta ccttttctcc tggacctgct gtattttcga tgtgaagatt ttctaatacg
     5461 acattcgatc cttctaagaa aaaagtagca cttcggaaag tccctaactg gctaccgtca
     5521 agatactcct gttttgcaaa acgattaccg gagataacta ctgttcctgc tcccacaagt
     5581 ttaaaattgg ataatctgct ttctatatat tcatcatatc ttccttcttt tactatcaag
     5641 acctttggat ggctatccgg caggcctgcc aaataatcga ttccttcttg gatagtttca
     5701 aaatccgtcg gggtatctat agcattcttt ccaattatca gtttgtcttt catcctcata
     5761 aatgctccct tatgaattgt ttccaaatat ttctcatttc ttgtgtttcc atatgaccgc
     5821 ctgttaaaga aaaagaagaa aagttttctg gactacccat tgattgatac tttttgtcca
     5881 gcccttttct cagcttcagt acgccttcgt ccaagcacat cgtatcgttt tttccaacta
     5941 agctaagacg agggcgcgga gcgatttttt cttggattgc taacgttgta taagctgtca
     6001 atagatttgg cacatagtaa taaaacccat gatgatctag gcggcgatgt agcaaaagtg
     6061 tttcgatgtc cacctgtgcc gctagatcaa tacaaacttt gacacgagaa tccaatgctg
     6121 tcacccacca gcttaataag cctcccatag aaagaccaat cgttgcgagc cgttgagtat
     6181 ctgtgtcttc ccttgtctcc aaataatcga tcaagctgat cacatcataa atacgcattc
     6241 cccataacgt ttttcctgtt aataaaaatt gtttgaacaa ctcgctttca gaaatacctc
     6301 cgcgttcttc aaatccccaa gcatcaatcg accaaacagc atatcccatt cctgtcagta
     6361 cttctgcaaa agatggagaa gctaagtaag gagcaccatg gatcaattca cttttcccga
     6421 ctgaaaaatc tccgccgtga gaatgcaggt aaaggaccgt cggaaacggt ccttcttttt
     6481 ctggataagc atagagggct ggtactgttt ccaattcatt taagtacaat tgatagtatt
     6541 caagcataaa accgttttgt ttttctcggt ataatcgttt tccactaggg gtatgtctgc
     6601 tagatggagc acctaaaaaa tcattcaact gcataagcaa agtttttacg aacaaccagt
     6661 tcatccatat tttctgattg atttgattgg aatttctcaa tactgatttc tttagtattt
     6721 tctaatagaa atgctggtcc ctcgtgattt tcaatcgaca tctggttaaa ggaaacctta
     6781 cccgcaaagc caatataaaa tccttgtctg ctcatttcct ctattcctgt catcatagca
     6841 ggaacaccgg gaactgcatc ttgggccatc gagacatcga tattgtcaaa tgtgatttct
     6901 tgtatgaatt gttccgctaa tccataaata aaacctgcag aagcatggac attccttgcc
     6961 gtgatattcg aaaaatggat ccttctgaat gctgatgtcc gttcatctat cggataagcc
     7021 tttttttccc atacatacgg ttctttccct cgcggaccac agaaataata aagattcagt
     7081 ataaatggac acatcacatt atccatgact atattggata ctcgaatatc ttcaacgatc
     7141 ccgcctcgac ctcgtctaga tttcaatcgt attcctcgat cagtttcttg gaagatacaa
     7201 tttgaaatgg taatattacg aatactacca ctcatttcac ttcctaatac gacaccgcca
     7261 tgtccatgga ccatcgtgca atttgtgatc gtgatatttt cgcaagcgat cctttcgtac
     7321 gtatcttcag tccctgcctt gattgcaata cagtcatctc ccacgtcaat atggcaatta
     7381 ctaatacgca catttttgca tgattctgga tcaattccgt ctgtatttgg tgaatccgct
     7441 gggttcaaga tcgtcaagtt atcaaacgtt gcattactgc aaagaatcgg gttgatcgtc
     7501 caacttggtg actggatcaa tttaatatct ttcacagtaa ttcgatgaca gttatggaaa
     7561 ctcatcaatt taggacgagg ataagcgaga ttatctggtt catttctaaa agtatgccac
     7621 catttttttc catttccatc taatgtaccg aaaccagtga cagagatgtt ctctacattt
     7681 tgagcataga tacaggaagc ataaactttt cgatggactc cttcccaacg tgaatgaaca
     7741 acaggataat cttttggatc gtcagagaat ttcagaaccg ctcctgcgct taaatgaagt
     7801 tcgacattgc tttttaaaaa taaagctcct gttagaaatt cacctgctgg aacaactacg
     7861 gtcccgcctc cgtcacttgc tgcagcatca atggcttgtt gtattgcttc agtattcaat
     7921 tcatcaattg atgcgccaaa tgtcaaaata tcatacatat ttataatgcc tccacttcaa
     7981 tcaatgcttg caagaatgca ccaacgcctt tttgatcatt acaaataatt ggttcactga
     8041 tgtagtaagc atatgaacca tctcgctgat ctgctccgcc taaccctgct acttgacagt
     8101 ttttattcaa gttcagccag ccttcttttg tttccagaac aaattcatca agtaatcctt
     8161 gatgtgattt cttcacttgt ggaagccatt cttctttgtt cagcagtccc aaacgtattc
     8221 ctttagccat tgcgtaaaca aacatactac tgccggaagc ttccagataa tttcctttcc
     8281 gttttgtttc tgttgttact tgataccatg tcccgctgtt ttcttcttga tatggaagta
     8341 atgcagccag tgtcttttct agaatttctt tcagcaaaga acaatctgtt tcttcatgaa
     8401 gtaattcata ggtatctgct gcagccatca aataccagcc aatcgatcgg ctccaaaaat
     8461 gatgggatag acctgttttt ggatcagccc atggttggac cttcttttca tcccatgcat
     8521 gaatcagcaa atttgttttt ggatcgaata gatgtttata actgatttca aactgtctca
     8581 aaacatcttg gtaatctttt tcttcactaa aattctggat aaattcagca taaaaaggag
     8641 aacccatata taagccatcc aaccaaattt gataaggata aatttcttta tgccaaaaag
     8701 ctccttcgga tgtgcgtgga tggtttgcca actgctggga aaggagttct gctgcttttt
     8761 tatatttttc ttcgcctgtt tctttgtaca ataaaaagaa aagctttcct gtatttacat
     8821 ggtcgatatt gtattcttca acgctgtagc cacggatcgt tccatcttct tgcacgaaat
     8881 agtcgatatt gtcttttatg aaatcaaaat aaattttttc ttgggttcgt tcccaaagta
     8941 attggaaccc tttaagtacg acgccatagt cataagacca ttttccttta taccactttt
     9001 cttcatataa gcgaggtgtt ctttccatga tactttttgc taatttcttt gaccagtttt
     9061 ccattatttg ctctccttta aaaattaaaa taggatttgg gacaaatacc aagaagtatt
     9121 tgtcccaatc gtctatatta ctgtgccgct gctttgtatg cttcctcgta ctcttgtgtg
     9181 attcgatcgc ctccttgtgc tttccagtct tctacagctt ttttgaatcc atcttcgtca
     9241 agctgtccca tgatgtattg caccgtagca tcaatgacga ttttttcaat ttctgatcct
     9301 acttcattgt tcgtcggtga atccaatggc acagaaggat ccattacagc aaattcttca
     9361 ttttctttga tcaattgatt agagaattct ttttcagggt ctgtatcttt gaaagagaac
     9421 gtcacttcac taggacgaga gcttgataac ggttgcacct cttgttgcca aagatccata
     9481 tcgataaatt cgatagaacc atcatcattc aattcataat gcgttccttc gataccgcca
     9541 gtcatcaatt tatacacatc ttcttctaaa agatcatcaa caaattgaag taatttcttc
     9601 aattgtgctt cgtcttttac ttgagatttg ggaaatgcta gcaagccgcc aataccattt
     9661 ccttctgacc aaacatgata accgccgcct tcaccatctg agatcttgtt tacaggcact
     9721 aattccatat catcttgaat accttgaccc atcgtcttca gattcttgat atccaccatt
     9781 ccagtgtaga ttccagttct tccttgtgca aattgttctt gctgatcagt cttttgggta
     9841 actgcaaagt cttgtgccaa atagccattc tcaaatagat ctcttgaaaa attcaaagct
     9901 ttcatatact cttctgtatc aaattctgga gtgaatttgc cattatcgtc tacagcccag
     9961 ccatttggtg ctccaaaata agaagtgaaa agtttaaagc tggtataacg aatatcacta
    10021 ggcgcacgat cgccaaaacc aactgtgtcg tccacaccat ttccatcagg atcgttttct
    10081 gtaaattgtt tagctacttc ataaagctca tccatggtag ttggaacatc tagtcctaga
    10141 ttatccaacc agtcttttcg aatgactaag ccagcacgag cgtagtcttt ttgtatcggt
    10201 acgccgtaca aggcaccgtc gatactcgca gcttcgattc tatcttcaga aatttgactt
    10261 aaatttttat agtcttttaa atattctgat acatcccaga atacgcctga ttttaacgca
    10321 ttgcggacag aagagttttt caacatcgca atttgaagtg taacgatatc tcctagttca
    10381 tcagaagcca atgcagttgt taatctttct tcttttgaag catcaggaat ccagttgaat
    10441 gtcagttccg tatcggtttt ttcttcgatc aagttcacaa tcgtgtctgt aggcggtgaa
    10501 gcatgatgca aaatatttaa ccaagtgatt ttagcttttc catcttctgt ctctgaagaa
    10561 ctgttacttg aactgctgcc gcatgcggca agtgctagaa ttcctgcaaa actaagacct
    10621 aattttacga gcgttgcttt tttcataaat tttctccttc actgttttaa taatattttt
    10681 gttttcttct gttttttctg tatatgagtt cttccattcc aaatggcaaa atatagaatt
    10741 tgctgtccac gtgactgcgg aaaagaagag actgccaccg ataaaggtta aaacacctgg
    10801 cattgtttgg atgatcatca gataaatccc taccatactt aatatcaaca ctaatgagta
    10861 atgaagatgt gagaatccga ataaaaatgc cattttcatt ttgaaaaaat atcctttcca
    10921 atcataatga gccatgattg ggaaaatata gagtgctgag agcacatata tgaacgcagc
    10981 aatatacaaa gcgatcctaa gataccaatt gtgtatgaaa gaaagatcca caagtaaaac
    11041 aatcccgatg gcgccatata tccagctcat cagcaaggtt tcacgatagt tttctttaaa
    11101 atatgtgaag tacgttttcg tcaaaggaaa cgtttctttt gagcgcagcc attttctaat
    11161 cactgcaacc atcgcatacg atgcaggacc gaaagtaaaa actcccaaac ctgcgatgac
    11221 taaaccgccc cacagaaaat tcaaataaac caactgtaaa atacgattta gccttccgtt
    11281 tattttatct aatgactgca gcatatcttt ccctctcttt ctatcttagt aaacgccgtc
    11341 ttctcctagc tttttggcaa tcttattagc taaaattacc ataaacaatc ctacgacccc
    11401 tttaaacaat cccattgctg tactgaaact taactgtcca ttcttcaatc cagctgtata
    11461 aatataggtg tcgaaaattt ctgcaacgct tcgattcaat gaattcaata acaagtacat
    11521 atgctcgaac cctaagtcta atgtctgacc gatttttaga atcagcaagg taatgattac
    11581 tggacgaatt gccggcaatg taacatgcca tgttttgcgt aagcgtcctg ctccatccat
    11641 ttctgcagct tcatacaact gtgtatcgac tgcagttatc gctgaaaggt agatgatcgt
    11701 agaccagccc aactctttcc agatcacttg tccgatatat actgtgcgtg tccattcagg
    11761 ggatgttaga aaactgattt tttcaaatcc cattgttgca aggaattcat tcaatacccc
    11821 tccgtcaaca ttcaaaaata cataagtaat cgaaacaatg attacccaag acatgaagtg
    11881 agggatgtaa atgatcgttt gaatcccgtt tttcaatcgt ttgttcgtta cttcatttaa
    11941 tagtaaagac aaaatgattg gcatcggaaa aaacacgaag atatttaatc cgaacaagat
    12001 caacgtattt cttaataatg taaagaatgt cggttctgta aacaaacgaa tgaaatgttt
    12061 gaaaccgacc caaggacttc ccattattcc taaaaatggt tgataatctt gaaaagcaat
    12121 aacgagtccc cccataggaa gatacctaaa aatcaagaaa taaaggactc ccggcagaat
    12181 catcacatat aaaaatttat tcgttttgat tcctgcaatt attttttttc gtcttctgag
    12241 cttttcttct tttttcgata gcgtcagttg aaatggttct tgtttcttta cactttccat
    12301 aagcccctcc ttagttttgt gaatgtttgc tgcttatgac ttcatgatag ataactatct
    12361 gaattacgta tataatcatt tttatcgtaa gtaaaaacgc ttacatagca atgatttttg
    12421 tgctatacaa aacgataaag tgtatttttt cacaaataaa ctacgcatag caaaccttct
    12481 atttttttct atctgacact tagcatagga aatgattata tacgtaatct ttcaccgatg
    12541 ataaattgaa catatagaaa cagaaacatt taatttattt tgaggaggag cctatctata
    12601 tgaaaaagaa agtctctata ggagaaagaa gttttacact tttcaacaat acgtttctaa
    12661 ttttgctagc tttgatctgt atcgttcctt ttttaaatat catcgctaca tcttttgctt
    12721 ctacacaaga agtcgtcgcg aaaaagttta tcctttttcc tactaccttt tctttagacg
    12781 cgtatcgcta cattctttct acgccgacca tttttagagc acttgctgtt tcgattggtg
    12841 ttacaggtgt cggaacgatc gtcagtatgt gtgcaacttc actgatggcc tacggtcttt
    12901 ccagaaagta tttgtttggt cgaggtttcg tcaactttct tgttgtattt tctatgttgt
    12961 tcagcggggg gatgattcca acgttcttag ttgttcgttc tctagggctg gtcaattctt
    13021 actgggccat gatcctgcct gtggctgtta atgcaatgaa tatgatcatt atgagaaact
    13081 ttttccaagc cttgccggat agtttggaag aatcagcaaa aatggatggt tgcacagatt
    13141 ttggtgtatt tttcaaaatt atgctgccat tagctttacc gtctattgct actatctcgc
    13201 ttttttatgc tgttacttac tggaacactt atatgactgc tattttgtat atcaacgatt
    13261 catctaaatg gccgatacag attcttttac gccaaatcgt cattgtatca agtgggatgc
    13321 aagcagaaag tagtgccgtt gatgtgattc caccagcaca aacagttaaa atggctgtta
    13381 tcgttattgc aacagtgcct atgctgattg tttatccttt tgttcagaaa tattttgtca
    13441 aaggagcact cgtaggttct gtcaaagggt gattatgtac tgaacgcaaa atggtagttg
    13501 tgtcagaagt ggtcagcttc aagaaataag aaggcattta cgaaaattgc ttcttaaatt
    13561 tttgtgaaat ttcggcttat ttttcgaaga atctactcct ggaacaccgt gtatcagagg
    13621 ttgtgaaaga gacgttcagc cttgagcgat gataaaaact ctcacaaaca ttgcgacgag
    13681 taacccacag gagcaataag aagcgatttt gaaaaatagc ttcccatatt tcacaaaatt
    13741 atgccttatt gtcgatagcc tgtctcttga acgccattta ttcgtaaaaa agaactgtga
    13801 caagactttt gtcacagttc tttttgcgtg cttagccttg aaatttaact atccataaat
    13861 acaaatacta gggttattct aacttctttt attgcttgaa tcagtccaca agaaaaacag
    13921 cctcttgatt aaacggcgtt tatcttgttg attctgcggc aataaaatca gccagcagca
    13981 aaacatgaag tgctgttttt tgctgtcctc ttttattgct tgaatcagat cacaagaaaa
    14041 acagcctctt gattcacaaa tatttttttc gatattctcc cggcgtaatc gattccttct
    14101 ttttaaagaa acgaataaaa ttttgcggat ttcgatattg caatctttct gcaatctctt
    14161 ttacggagag gttagtctct ttcaaccatt tttttgctac ctctaaacga tgattttgaa
    14221 caaaatcacc aaatttttct cctgtttcct ttttaaatac attacttaaa tagttagggt
    14281 tataatgcaa cctatcagcg atcacatcta aagacagatc acgatcatat tctgtgtaga
    14341 cgatatgcat aatattctcc gacaaatttt taaattcatg atcagtgatt gcttgagagc
    14401 attctgtgat tggcagaact aattgttcta aaacaagttt ttctagttct ttgggatgat
    14461 aattgttcag tgcttttaca taaatttgtt tcatgttatc aaagtttttt gaatctactc
    14521 ccaataactg tcctaattga acgagttcat tgatcaagcg gaccgaaact acttcccgac
    14581 tcaaaggatt tttattttgt gcaaacaatg catcaatcaa ttcatgtact aaattcttca
    14641 cttcctgatt tccagaacga atggcttcaa acaatcgatt ttgttcttct atcggatatc
    14701 taataagtgt tttttcatga gcagcaggaa cgatttcctg ataaaaaata attgaacttg
    14761 gaccagtatt tacacgataa tacagggctt cttttgcccg atcaaccgat tgcttgcttt
    14821 cattcagcgt ttcgaaccgg ttactgatgc ctatactgat agttaaattc aagtattctt
    14881 ttacacgttt ttgaatgagt cggcaatact ccatgacttt tttgcttgct tctgcatcat
    14941 ctttatccat tcgataaata gtggcttgca tttcttcatt caaaacgatc ggcagcattc
    15001 gattttcctt tggcacaatt tccgtgatca tgttgttgat cgctaaaagt agcaaatcac
    15061 gctccccacc gtgactatct tctaacagat cgatttgaac caaagctgta taatagcagt
    15121 cattttgaga actataacca aattgctgca accgctgatc tagttctcgc ctttctactc
    15181 gatttctaaa taaactaagt acaaatagcg tttccagctg tggcttttgg gtaactaagt
    15241 gtgcacttaa cgcttctttt tctcctatga tccgactgac tgattgagcg accaaatcta
    15301 gttctttccc actgaatcct tttgatttaa gattcagacg ttcctgtatt tgagaaatcg
    15361 gacgggcaaa tctgtccgaa aataagtagg aaaaaagacc aatgagtcct accagtgtta
    15421 aagacactgc aactagcccg attctcagat tgcggatcat tgcgccaatt gcactagagt
    15481 caacttctaa tctgtaaatc caattattat aatctgattt cagtactata tacgttttat
    15541 ctttttctct tatcactttc agctgattgt aatctagatc cacgtccgtt aatacagttg
    15601 gttgctcttt attttttact gtctgagtag taaaaaggtt gccaacttcg ttttcaatag
    15661 aaagtatgtc ttctccactt tcaccaacaa cttgttctat tgtatgtttc tctatcgtag
    15721 caattcccaa tgcatatttt tctttttcaa acattggcaa agaaatcacc atttcgattc
    15781 ctttgttttt cttttgccaa aataaattat ttttgttatt tatgtacttc tgttgatact
    15841 tttcaacttc ctcttcggtc agttgactca acgatccatt gattactccc catttaccag
    15901 ccaaactgac taaatcataa ttattatcat ccataacaat cgtttctata aaatttagtt
    15961 ctttcctgat ttcagaataa gtggagtaat ctgtatatgt cagcggatgc ttgaatatgt
    16021 ccgagaaatt tttggtcgaa ccatacgtag aaaacgaata ttcaatcgtt ctaattttcc
    16081 attcaacgtt agttttaact tgcctgagca aattcttttt gtctgttaaa taactcgatt
    16141 cgatcgattc ataggtattc tggtaaatga ataaactaaa tcctatgaca agtacggctc
    16201 ctaataaaaa catcggtaaa aatattttat aaaatactgt acttttcttc attttcttcc
    16261 tctcctcctt ttaaaataaa aggctttcat ttttctgtaa aattgatact catctaatta
    16321 aatgagcatt ccccgctttc aagaatagca taattaaaaa taaaaatcca agtaattttt
    16381 aacgtgtgca atcttttcat atcaaagatc tattgactta tcgtagttat cgtaatatga
    16441 tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat ggaatggtaa cgatcaaaga
    16501 tatcgctaaa gcagctggcg tttctcatac gacagtttct cgtgctttga ataacagcag
    16561 tttaataaaa aatgaaacaa aacaaaaaat acgacaacta gctaaggaaa tgaattattc
    16621 gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa tatctaatcg gactattttt
    16681 ctcaagtttg cataaaggaa cttcatcaaa tttcctagca gattccattg caggcatcca
    16741 ttcagtatta gacacaaatt acagtttatc tgttgaaagt atcgatgttg ttgatttaac
    16801 agaaatcaac ctgcaacgct acgatggact gatcgttatg agtcaaagtg atgatgatca
    16861 gcctttcatc gactatctca aagaaaaaga atttcctttc gttgttgtca atcgtcacat
    16921 caatgatcca gcggttatca acgtcactgc cgatgatgcg gccggtgtta tgcaggcagt
    16981 agattacggt attggatgcg gacatacgaa gatcgcttat attggcggaa agaaaaattt
    17041 tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt ctaaaaaaag cgagcatccc
    17101 tgtcaatcta gcctattttt taacaggaga ttacagctta gaaagcggcc tccaaaacat
    17161 ggaataccta ttatcacttt ctgacctgcc tactctggtt ttttgcggca acgacgacat
    17221 agcgattggt gcattgagag ctgtacattt agctggcctc cgtgttcccc agaacatttc
    17281 tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc aatccgccgc ttaccaccgt
    17341 acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag cttttactag atttgatcga
    17401 aggcaggacg atcgaacaat ctaaaattga gctctccacc ttgttgatcc ttcgtgagtc
    17461 gatcaattac ttgtaaacta caagtaattt tatataaaaa tgttaacgtg tgcataacac
    17521 agaaaggaag tttatatgaa aaaactgaca aaagaattag tgaaaaaaaa cgaagcacct
    17581 gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg gatttattga ttggcagatt
    17641 cagcagctga ataaccaaca attgtttaaa ggtaatgttg caatcattca acctttagaa
    17701 caaggtcttg gaaatatgat gaaccagcaa gataatttat atacggtcat tttacaagga
    17761 ctcagcaatg gcgaaatcgt tgatacttcc gagattatca ccgtagtaga acaaatgatc
    17821 aatccttatg atgaatggga tgcttatcta tctctagcag aaaatgacga cttggcctat
    17881 atcgtctcca atacaacaga ggcaggaatc caattcgtgg agactgataa tttaacagat
    17941 actcccccac agagcttccc gggcaaactg acagcctttt tataccgacg tttcaaatta
    18001 aacaaagctg gctttaccat cattccttgc gaactgattg acagaaacgg cgaacgtcta
    18061 aaagaaatca ttttacgcta cgcagaaaaa tgggatttag aacctgcttt tgttgagtgg
    18121 ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc gcatcgtccc aggttatcct
    18181 cgcgaagatg caggtgtttt caatcaacgg cataactatg aagatcaatt gatggtcaaa
    18241 gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag aattggagaa tcaactacca
    18301 ttcaaacagg ctggcttgaa tgtcatcatc acagatgata tgactcctta tcgtgaacgt
    18361 aaggtgcatt tgcttaatgg accgcataca gcgatggtcc ctcttgcttt gttggcggaa
    18421 ttggaaactg tagaagaagt aatgaaagat cctttattct ccgcatatgt tgatcagtta
    18481 ttcaatctag aattgattcc tatgctttct ttacctaaag atgaattagc catttatgct
    18541 gaccaaatca aagaacgttt cttgaatcct tttgcccatc acaaattaga agcgatttca
    18601 ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag tgttcaagaa gtatatcgaa
    18661 gaacaaaatc aggtaccacc gttaatcact gtttcactag ctgcgctact tcttatgtat
    18721 cgaggcgatc aagtaaagcc acatgatgat gaaaagacga taagcgaatt tactgatgct
    18781 tggtctgata atggaacagc tattcctcga ttattgcaaa acgctgcgct ttggggagaa
    18841 gatttgtctc aaataccgaa tgtgacagat acagttcaag aaatagctgc aaagatcgat
    18901 caaaaaggtg tccggtcagt attacaagaa atgaaaggaa cgatggtcca tgcttgaaac
    18961 cctgcaacca acaatgaaat taaatcgacg agacagtgtg gtcgtcgcat taacccctat
    19021 tccaaaacaa acacaactga cgattgatca gcaaacgatc accactcttg aagatatccc
    19081 gcaaggtcat aagattgcat tgggtgattt gaaagctgga gacaacgtga tcaaatatgg
    19141 ctatccaatc ggtcatgtga cgacagacgt aactgccggt caatggctgc acactcataa
    19201 tgtaaaaacc aatctttctg gtgaattgga ttatcgatat gaaaaagatg tccatccaag
    19261 tcattacctt tttgagaacc gaacatttca aggttacttg cgaaaaaatg gaaaagtagg
    19321 gattcgtaat gatttgtttt tagtacctac cgttgggtgt gtgaatggca ttgctgaact
    19381 gatcgtcaaa cagttcaagg aaaaacatcc agatctggga cagtttgacc atattacgat
    19441 tttaaagcat ccttatggct gttcgcagct tggaaaagat catcaaaata ctcgagaaat
    19501 tttagcggac gcagtaaatc atcccaatgc cggaggagtt ttagtatttg gattaggttg
    19561 tgagaacaat acagttcctg aattcaaaaa aattttaggt gaatatgatg aagaacgagt
    19621 gaaatttcta gtggctcaag atgtttacga tgagatcgaa caaggtgtgg ctctattaga
    19681 agagctttta acagcggcgg aaaatgacca gcgcatatca gtcccgctct ctaagttgaa
    19741 tgtcggtttg aaatgcggcg gttctgatgg tctatcggga ataactgcta atcctttact
    19801 tggtgcattt tctgactact tgattgctca aggaggcagc acgatcttaa cagaagtgcc
    19861 agaaatgttt ggcgcagaac aagtattgat ggcacgagcg gaaaatgaag aagtttttga
    19921 ctcaatcgtt gacttgatca atgacttcaa acagtatttc ttgtcttacg gggaaccagt
    19981 ttacgaaaac ccatctcctg gtaataaagc aggtgggatc actacattag aagataagtc
    20041 tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt gatgtgctac aatatggcga
    20101 aaaaatcaga aaaccagggc ttagtttatt gcaagctccc gggaatgatc ttgtagctgc
    20161 ctctgctctt gcttcatccg attgccagtt ggtcttgttt accacaggaa gagggactcc
    20221 gtttggctca tatgtcccaa cggtcaaggt atctacgaat acaactcttt ttgaacgcaa
    20281 aggtcattgg atggatttta atgcaggcgt cttattagaa caaccaatgg aagtaatctt
    20341 agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga gaagaaacga aaaatgaaca
    20401 aaatgacgtt cgagaaattg ccatttttaa aaacggagta acactttgat tttcataaaa
    20461 cagtataaaa agaaagaagg aagatttatg tttttatctg atgatttttt attaaaagac
    20521 gattgggcga aaaagcttta ccattcctac gcaaaaaaca tgcccattat tgattatcac
    20581 tgtcacttag atccaaaaga aatttacgaa aacaacaatt tcagcaatct gacagaagct
    20641 tggttaggag gcgatcatta caaatggcgc ttgatgcgag cttgcggcgt gcctgaagaa
    20701 aaaatcacag gaaatgcttc tgattttgaa aaatttttag cttggtgtca aaccgtacca
    20761 aaaattatcg gaaatccgct ttacagttgg tcacatcttg aattgaagcg tttcttcggt
    20821 attgacttgt tgataaatga agaaaatgca gagaaaattt ggacacaagc aaatgaaatt
    20881 ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa aatcgaatgt agaagtgatt
    20941 tgtacaacag atgatcccgc agacgacttg cactatcatc agttgcttgc tcatgaagaa
    21001 ccagatttga aggttctacc ttcattccgt ccagataaag cactcaatat tgaaaaagaa
    21061 ggatttaatg catgggtcac actactggaa gaagcagctg accaaacgat caccacctat
    21121 caagaactga tagaagtctt agggcagcgg atagaatact tccatcaaca tggatgtcgt
    21181 gtttccgacc acgctttaga catcttacgc tataaagaag cggatgaaac agtattagaa
    21241 gagatttttc aaaaagcacg agctaacaaa ctattatctt cagatgaaat cgatgcttat
    21301 cttacagaaa cactgataca tttgacccac ttctatcatt ctcataattg gacgatgcag
    21361 ctgcacatcc atgcttatcg taactgtaat acacaaatgt tggaaaaatt agggcccgat
    21421 accggctatg atggaatgaa tgatctttct ttgactattc ctctgcaaaa attattgaac
    21481 cgtgcagaag aaacggatca gttgccaaag acgatccttt attcattgaa tccgaatgac
    21541 tatcctgcga ttttagcttt gatgggctgt ttccaaaaag aacagaatgg aaaactgcag
    21601 ttaggttctg gatggtggta caacgatact cgagctggta tgcgcgatca attgacacaa
    21661 tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc tgacagattc aagaagtttc
    21721 ctgtcctaca ctagacatga atatttccgt cgtgttcttt gtgaattttt aggcgaaatg
    21781 gtcatgcgag gagaagcacc tgaagatgaa caacttttag gaagtctagt tcaagcgatt
    21841 agttatacaa atgctaaaaa ttactttggc ttttttgcct aattgattaa tagtaacagt
    21901 aacgaatcaa aaacaggata gtgaaataac ggaatccgtt tatccacgtt ttagagaaga
    21961 agacggacca a
//
LOCUS       DANKAZ010000040        21924 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000040 DANKAZ010000000
VERSION     DANKAZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            89..1258
                     /locus_tag="PWY67_001464"
     CDS             89..1258
                     /locus_tag="PWY67_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2057759.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1832..2401
                     /locus_tag="PWY67_001465"
     CDS             1832..2401
                     /locus_tag="PWY67_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL2057760.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2388..3248
                     /locus_tag="PWY67_001466"
     CDS             2388..3248
                     /locus_tag="PWY67_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2057761.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3438..4142
                     /locus_tag="PWY67_001467"
     CDS             3438..4142
                     /locus_tag="PWY67_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2057762.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            4147..5982
                     /gene="walK"
                     /locus_tag="PWY67_001468"
     CDS             4147..5982
                     /gene="walK"
                     /locus_tag="PWY67_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL2057763.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5979..7295
                     /locus_tag="PWY67_001469"
     CDS             5979..7295
                     /locus_tag="PWY67_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057764.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            7296..8165
                     /locus_tag="PWY67_001470"
     CDS             7296..8165
                     /locus_tag="PWY67_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL2057765.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            8220..9029
                     /locus_tag="PWY67_001471"
     CDS             8220..9029
                     /locus_tag="PWY67_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2057766.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(9132..9761)
                     /locus_tag="PWY67_001472"
     CDS             complement(9132..9761)
                     /locus_tag="PWY67_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL2057767.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9931..11223
                     /locus_tag="PWY67_001473"
     CDS             9931..11223
                     /locus_tag="PWY67_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2057768.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(11299..11961)
                     /locus_tag="PWY67_001474"
     CDS             complement(11299..11961)
                     /locus_tag="PWY67_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2057769.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            12186..12470
                     /gene="groES"
                     /locus_tag="PWY67_001475"
     CDS             12186..12470
                     /gene="groES"
                     /locus_tag="PWY67_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL2057770.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12521..14146
                     /gene="groL"
                     /locus_tag="PWY67_001476"
     CDS             12521..14146
                     /gene="groL"
                     /locus_tag="PWY67_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL2057771.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(14235..15383)
                     /locus_tag="PWY67_001477"
     CDS             complement(14235..15383)
                     /locus_tag="PWY67_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2057772.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(15485..16069)
                     /locus_tag="PWY67_001478"
     CDS             complement(15485..16069)
                     /locus_tag="PWY67_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL2057773.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(16196..16627)
                     /locus_tag="PWY67_001479"
     CDS             complement(16196..16627)
                     /locus_tag="PWY67_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2057774.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16645..16965)
                     /locus_tag="PWY67_001480"
     CDS             complement(16645..16965)
                     /locus_tag="PWY67_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2057775.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            17265..17405
                     /locus_tag="PWY67_001481"
     CDS             17265..17405
                     /locus_tag="PWY67_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057776.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            17703..17960
                     /locus_tag="PWY67_001482"
     CDS             17703..17960
                     /locus_tag="PWY67_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HDL2057777.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(17931..18239)
                     /locus_tag="PWY67_001483"
     CDS             complement(17931..18239)
                     /locus_tag="PWY67_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057778.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            18317..19063
                     /locus_tag="PWY67_001484"
     CDS             18317..19063
                     /locus_tag="PWY67_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL2057779.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            19078..19281
                     /locus_tag="PWY67_001485"
     CDS             19078..19281
                     /locus_tag="PWY67_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2057780.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            19386..19685
                     /locus_tag="PWY67_001486"
     CDS             19386..19685
                     /locus_tag="PWY67_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057781.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            19685..19867
                     /locus_tag="PWY67_001487"
     CDS             19685..19867
                     /locus_tag="PWY67_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057782.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(19873..20349)
                     /locus_tag="PWY67_001488"
     CDS             complement(19873..20349)
                     /locus_tag="PWY67_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057783.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            20402..21127
                     /locus_tag="PWY67_001489"
     CDS             20402..21127
                     /locus_tag="PWY67_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL2057784.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            21301..21642
                     /locus_tag="PWY67_001490"
     CDS             21301..21642
                     /locus_tag="PWY67_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057785.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            21635..>21924
                     /locus_tag="PWY67_001491"
     CDS             21635..>21924
                     /locus_tag="PWY67_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2057786.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         7468 a         3639 c         4385 g         6432 t
ORIGIN      
        1 ttgaaaatac aagatgaagg taaaaatcat gctataataa aatagtcaaa atttttgccg
       61 atatttcagt ttaagaggag agattgaatt gaaattaaga gcaaacataa ttgaaaaaag
      121 aggaacatta caagtagttg tatcttataa agactctaac ggaaaaatta gacaaaagtg
      181 gaaagataca aaattaaaaa agaaaggaaa taaaaaacga gcagaaaaaa ttagagatga
      241 atttttacta gaattggaaa gtcaattagc tatggaatgt gaatgtgcta atccaaattt
      301 attgttttat gactatttgc ttaattattt ggaagtatca aaaaaacaag tagcatttaa
      361 cacatatgtt gggtataagc attacgtcca taatcgaatt tacaagtttt tttatcctaa
      421 aaaaattaaa ctaaataggt taaagcctta tcatttacaa gagttttatc aatatatgtt
      481 aaatgataac tgtacagcaa atacggttat tcattatcac gcatttatac gcaaagctct
      541 tcaagaggct gttattacag aattgataag tacaaatgta gcagataaag taagaaagcc
      601 aaaaaagaaa caatttgttt ctcaagttta taatcaacaa gagatcttga aattacttga
      661 cattttgtca gaagaaaaac tctatttggt agttctttta acagcatttt atggattgcg
      721 aagaagtgaa gttttaggat taaaatggtc tgctattgat tttttaaaca aaaaaatcat
      781 gataaaccat gtaatcatag aaaatccaga gaatatgtca cagttaataa agaaagatca
      841 aacaaagaac tcttccagct atcgtacatt acctttggtt gaaacgattg aacgagcatt
      901 agtaaatcaa gctaaatggc aacaagataa tcgtattctt ttaggagatg attatatttt
      961 agaagataat gagtacgtat ttactatgga agatggacgg ttgatgaagc ctcaatatgt
     1021 aacgcatcgt ttgagtaagt tgattaagaa aaatggattg aaaaaaattc gctttcatga
     1081 cttgcgtcat tctaatgctt caataatgtt agatagtggc cagaatatga aatccattca
     1141 agaatggctg ggacatgcat cgtattcgac aactgctaat ttatacacgc atttaaccgc
     1201 aggagtaaaa gatcgtgcag cagaagcatt agaaaacgca tttgggttta aagaataaaa
     1261 aaacaaaaaa caatatcttt ggggtatcgc ttcttgtttt tagaattttt ttgttggcaa
     1321 tttgttggca gtatgttggc aatgaaaaaa ttcacaaact aaaaacctat ttataaagct
     1381 ttataaaact tttttcacaa atagagcatt tggttaagag tcagctgctc taccaactga
     1441 gctaacggcc cgtacaaaaa gtacttaata atagtagcac taagaatttt aaaatgcaag
     1501 actacattta aagaaaatca gaaaaatctt tacttcctta taggaaatca ttcatagatt
     1561 attcacattt gctcgctatt atataagcat accaacaaac aaagttcttg caagagaaca
     1621 aaccctaaca cttttttcat tttttactcc tttcccgccg tctccccggc gggtctcctg
     1681 ttcttaacga taaaagacaa gaaggcttag ccttcttgtt ttttgttttc atggaatccc
     1741 gctaatcaaa tcacctagtt aaagcaacta gattgaatgt ttatcataag tctgatatca
     1801 tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa atgcgtcagc aattaatgga
     1861 gtgggggaag gaactagaaa cattccgtct gcctcactgg gaagaactcc ctgacttaga
     1921 actttatatg gaccaagtca gaacgttagt tgatcgttat ctttcacctg tgatacaagg
     1981 agaaaagcat ccattgctta cctcttctat ggtaaacaat tacgttaaac taggtttgat
     2041 accagcacca gttaaaaaaa gatataacaa agaacacgta gcatttttac ttgcaatcac
     2101 tacgttgaaa caagtattga ctattcctga aatcaaagaa gggatattgt tccaaggaaa
     2161 aacagtagga attcgtgaag cctataatct attttgcgat gaacaagaag cagctgtgtg
     2221 gatggtcagc caattagcac aaggtaaatc tcatccgcaa aaattcgacc aggcaacccc
     2281 tgtagaatat attgctgtaa gagcggcaac tttatcgttt gcaatgaaac ttcttgcgga
     2341 aaaaacgatt gttctagaaa ccgaatattt aaaagaggag aaaacaaatg aaaaacaata
     2401 aaatcgcaat tttagtagat tcaggtacgg atgttccaga agaattgata aaaaaatacg
     2461 agatgtatat gatcccattg aaaattattt ataaagatca ggtatataca gataaaattg
     2521 acattacccc tgaagagatc tatcaacgtt tgccagagga aatccctggt acatctttgc
     2581 cggatggtga gtcgatcaca gaaatctttg agcaaatcaa aaaagacggt tatgaaaagg
     2641 tacttgcggt aacaatttcc agtggattga gcgggacata taatgtggtt cgtttaattg
     2701 cacaacagca agaagaattg gaagcatatg tactagatac gaaaaatatt ggtattggtg
     2761 caggtttttc tgcgatccaa gcagcaaaat ggctggaaga aggagtagaa tgggatcaac
     2821 tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt tttctttaat gttgcgacat
     2881 tggaatatct tcaaaaaggt ggacggatcg gtttagttgc ttctattgta ggaaccgctt
     2941 taaagcttaa cccaatcatc tcatgtaatg aagaagggat ttattacaca gtaggaaaag
     3001 ctaggggacg taaaaaaagt ttggataaaa cggttgctta cgtcaaagaa aaaatcggta
     3061 atgcaaaggt cttcaactta gctgttgccc atggggatgc gaaagaagaa gcaattgaaa
     3121 tggaagcgag acttaaaaga gagttcccac aagcaaacca aatctacttt ggccagattt
     3181 ctccggcatt agtagtccac actggtccgg gattattggg tgtcggtgta caagttttag
     3241 aaggataaat caaaaaaagg aaacactgag gtgaaaatca gtgttttcct tttttattga
     3301 agttagaaca aaaaataata aagtattttg cactttcatt ttaattttct cttttgtttt
     3361 ataggcaaag atgaaacaaa agcagaaata cggtataatg agaaggtatg acaataatat
     3421 ataagaggtg gctctttatg aaaaaagttt tagttgtcga tgatgagaag ccgatttcgg
     3481 atatcgtcaa attcaattta gccaaagaag gctatgacgt ttatactgca tatgatgggg
     3541 aagaagcctt ggaaaaagtt gctgaggtag aacctgattt gattttactt gatttgatgc
     3601 tacctaaaat ggacggctta gaagttgctc gtgaggtacg caaaacttat gatatgccga
     3661 tcattatggt aacagccaaa gattctgaaa ttgataaagt cctaggctta gagttaggcg
     3721 ccgatgatta cgtgaccaag ccattttcca accgagaatt agttgcacgt gtcaaagcaa
     3781 atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga ggcagcacct gcggaattga
     3841 cgattggtga tttgacgatc catccagaag cttacatggt gacgaaacga ggagaaacaa
     3901 tcgagttaac acatcgtgaa ttcgaactac tcttttactt ggcgaagcac cttggacaag
     3961 tgatgacaag agaacattta ttacaaacgg tttggggtta tgattatttt ggagatgtac
     4021 gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat cgaagataat ccaagccatc
     4081 caaattattt agtgacacgt cgtggtgtcg gttactattt gcgtaaccca gaacaggagt
     4141 aattggatga aaagaaaagt ccgctttttt cgatcagtca attttaagat cgctattacc
     4201 tttattctga ttttattgat ctcgatcgaa attattggtg cctactttat acgtggttta
     4261 gaacgttcca cgatcaatac atttattaaa gatatgaatc aaacggtgga atcattagca
     4321 acaacaatca gcccggaatt gaatcgaaaa gataatgcag atgatgaaga ggtaaatgcg
     4381 aatatcaaaa gatttattga aaacagtgcg actagcgata tcatcgaaat acgggtcgtt
     4441 gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg aacaaagcgc tgtgggaaag
     4501 aaaaatgatt atgttgatat caatgatttc acgactaaac gttatgttgc tttagataac
     4561 gacgataaac gggtgaatat caatgttcaa ccgatcctgt ccccgaccgg cgatgccgtt
     4621 atcggggctc tttacgtcaa aagtaatatc gaacaaaaat attcggaaat caataacaca
     4681 gcagtcattt tctttactgc ctcactaatt gcggcattta tttcaatgat cgtatctgtt
     4741 ttagttgctc gttcgattac acagccgatc ggtgaaatga gagaacaagc cattcgaatc
     4801 gctagaggag actacagtcg gaaagtcaaa gtgcatggac aagatgaact tggacagctg
     4861 gctgatacct tcaatcagtt agctgaacgt attgaagaag ctcaagatac aatggaagct
     4921 gaacgcaatc gtttggatag cgtgctatcc catatgacag acggcgtcat tgcgacagat
     4981 cgcaggggta aagtcattac gatcaatgat atggctgttt ccttgctaga tatcaaaaac
     5041 gaggaagcaa tcggacaatc gattttgaca cttttagata tcgaagaaga atatacgttg
     5101 cggaaattat tagaagatcc aaacgaaatg gtacttgacc gttcaaccag tacattggaa
     5161 agtgatcaga tgatcttgcg agtagacttt tctatgatcc gtcgcgaatc aggttttatc
     5221 agcggattag tagctgtctt gcatgatgtg acagaacaag aaaaaacaga acgggaacgt
     5281 cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac cgttgacaag tatgcgtagt
     5341 tatatcgaag cattgagcga aggtgcgtgg aaagacgagg aagtagcacc aaattttttg
     5401 aaggtaacgc tagatgaaac agaccgaatg attcgaatga tcaatgacct cttaaatcta
     5461 tctcgcatgg atacaggtaa tacccagctt caattagaat atgtcaattt caacgagtta
     5521 gtgaattttg ttcttgaccg gtttgatatg atggtgggaa atcaggaaaa aaattataag
     5581 atccgtcgtg aatttacgca aagagatctt tgggtcgaag tagatacaga taaaatcatc
     5641 caagtagtcg ataatatcat gaataacgcg atcaagtatt caccagatgg gggaacgatt
     5701 acttgtcgct tattagaaac gcataataac gtgattttaa gtatcaccga tcaaggattg
     5761 gggattccta aaaaagatct gaaccgagtg ttcgagcggt tctatcgagt agataaagca
     5821 cgtgcaagag cacaaggtgg gacaggactt ggattagcta tttctagaga agtgattaag
     5881 gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa aagggtcgac tttttatatc
     5941 tcgctgccat atgaacctta tgaggaggaa tggtgggaat gagaatctct gaaaaagtcg
     6001 ttcgtatagg actcattttg atggttgcgt taagcatata cttctcttat gcaatctggc
     6061 tgagcccagc aggaaaaagt tcaatcaacg tagatgagac aaacactcaa atgatcgagt
     6121 ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct tcatgttaca tggcaccatt
     6181 cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta ccgattgcaa ttgatcatcg
     6241 ataaagcaag atttggaaag ctttctgaag tagtttcagg agacaaatca cagtttgaac
     6301 aggtgaaaaa tctgaactct ggttttgagt tggcctataa tgcgcctttt ttgctaagag
     6361 aatacaagga agcatttgat ttagatttag atgtgtcgaa cttggcaaaa gaaagcgaaa
     6421 atatgtattt tactagaata caattcgatc aaggaaataa aaaaatccga tttttgaatt
     6481 attcgaacca tctcgtatat gaagcaaata tttcgttgaa ttggacagat atcaataaag
     6541 aattaacagc aaaagacaca aaatggacag aaatgacaac agaaccatcg acgatcgaca
     6601 cgcaatatgc tacgaaacat tcgatcaaac tgaagaagta tagttatatc ctgtcacagc
     6661 aaccctatac actttttaga aatgcttttt tccagaaacc agatagcgtg aaaaataatg
     6721 atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac aatgtctatc cattcggaaa
     6781 cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa agaaaatgct agtgtatttt
     6841 ctgaaagttt cccctatatt agcaaattag gcagttcttt agggaacctc agatattttg
     6901 accgcagtga caatacgatc gattatcgga tattcgtgga aggcttcccg gtttttggga
     6961 cagacagcag aggacagatt ggtgtggaag ttggcggtga aacgaatggt tctgtgcaag
     7021 tgaatatcca aaccagtttg aatacgattc aagtgccgat accttcagac gaagaagtag
     7081 aacttgcaag tacgaatgaa atcatccagc agttaaaagc taagggagca gatagcaaca
     7141 agattggttc agtgatcgtt ggctatacat ggcaaaatgt caaagaaacg aatcgtgtcg
     7201 ttgatctctt acctgaatgg tatgtgaaat ataaagataa ctggtattca gctaacgacc
     7261 ttctggaaaa attacctgga ttggaggcga attaattgga tttcaaaaaa atcgaatgga
     7321 tatttttcgt cgctttttta gggttgaatg tgtttttatt cagcagttat catgacgcgg
     7381 tataccaaga aaacaatgtc attcgctcta atcagactga atcaatccaa caacgtctgg
     7441 ctagcgacga tatcacttac acggggaaga tttcgtctga gaacaagcaa ggctattatc
     7501 ttagcgcgga gcagaccaat cttagcactt cattagcgaa ttatcgcaag gctggtaaaa
     7561 gcggcttgct ggcaagtggt gtgtttttag acggtgatgt tttgacgaaa tctttatcca
     7621 gttcagatgc tgaaaagaac gtgatcgatc cagataaaat tagtagttcc ttggaagatt
     7681 ttttatctaa caaagacaat attttattcg gtaatgatta tgtgtacacg aaaaactttt
     7741 ctcatttaga agaagaacca ctagaaatcc aagcaagcca agagtatgaa ggcatcccat
     7801 tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa aaacgataat gattatagca
     7861 tcacgaaata catgcagacg catttatctg atatcgagca gctgcgcgaa aagacggaac
     7921 tgtatacgga agaggaagca atcaatacat tgtacatcaa taacaaaata ccaagaggat
     7981 caaaaatttt atggcgtcag ctagcttatt cctgtattct gaaagtccgg gaaaaaaatg
     8041 tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga taaagtggtc caaattgaga
     8101 gcgtcaacgc tttcagcaat acgatcgtga caaacaatac tgtaccaaaa gtggaagatc
     8161 aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa gaagggaagt aggagtcaaa
     8221 tggctgaaga aaaaccagct tttaaaatca gtatcctagc aagtggaagt tcgggtaatt
     8281 cactttatat cgaaagtgaa aaaaaacggc ttttggtcga tgcaggactt agcggtaaaa
     8341 aaatcacttc cttgatggct caaattgatc ggacaccgga ccaattagat ggaatacttg
     8401 tgacgcatga acatcgagat catatacatg gcgtgggtgt cttggctcgt aagtatcatt
     8461 tggatgtttt tgccaatcag cagacttggg aagcgatgga accactgatt ggaaatgtac
     8521 cagtggaaca aaaacatcta tttgagatgg ggaaagtcag aacattcggc gatatcgata
     8581 ttgagagttt cggcgtctcc catgacgcag cggctccgca attttatcgt ttccataaag
     8641 aaaatcggtc attcgtcatg ttgacggaca ccggatattg cagtgattat atgcgaggtg
     8701 tcattgaaaa tgccgatggt tatttgatgg aaagtaatca tgatcttgag atgttacgca
     8761 tgggacctta tccgtggaat ttaaaacagc gtattttagg agatagagga catctttcca
     8821 acgaagacgg tgcacttgtc atgactgatg tgatcggtaa tcggaccaaa cgaatctatc
     8881 ttggccatct tagcaaggaa aacaatatga aagaacttgc tcatttaaca atggaaaatg
     8941 ttttgaaaga aaaagatttt ggcgttggac atgattttga aatctatgat acagatccag
     9001 atgaagcaac tgagctattt tcgatttaaa cagttcaatc aataaaaata taaaaagcag
     9061 aagataaaat caatcagggt tggttttatc ttctattttt atttttttag catattaaaa
     9121 aaataaccgt attatcgact gactacactg atgtgctgct tttcatgttt ctcatccagt
     9181 atctcatcta ctaatgcaat cgcgtaatct gccatgctga tcgtactgtc gctagcagcg
     9241 tttctttgta aataatcatc acttaacaaa taagtacctg tacgtttgcc atgtggatca
     9301 aataaagaag ctggactgat ataagtccaa tgtagatttt tagattgttt caattttaac
     9361 aatccttcgt acatattgaa agctgttgga tagaagggtg catttttagg tacttggtca
     9421 atcatacgag tcgtcttttc tgtatctaag aataacgaag aggcaccccc aacgacaatt
     9481 aagcgtgtat cggttccttc aagcatctca atcaaatggg acaaagaagt ttgatgcatt
     9541 tcttcttttc ctttgggagc gttgaaagca tcaacgacaa catcaaaaga tgcaagatca
     9601 gtagttgtca actcaaataa atccttttgt ataaacggaa catctgatgg caatttttct
     9661 ggatgacgca cgattgccgt cacgtctaaa tctctggaca atgcttcgtc taaaataaat
     9721 gaacctgcat ggccagtagc tccaatgagt gcaattttca taaaaaacct ccttttttat
     9781 tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga atccgatttt tgaaaagctc
     9841 agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag ttaaattaag aaaactgatt
     9901 gataaaaata gaagttgaag gagataagaa atgaaagagt ggttagatcg tgccttttta
     9961 ccaatgataa catctgttgt gaatcttaga cccttaactg ctttgcgtaa tggaatgact
    10021 tacacgatcc caataattat tgttggctcg ctgtttttct tatttgctca ttttcctgtg
    10081 gattacatcg ctcagtggat agacaaaaca ggattttcta tgtattttac acaagtatac
    10141 caggctacat ttgatgtgat agccttttgg atcgtttttg ccattgcata cgcttatgca
    10201 aaagaagaag acatcgatcg cttaccagtc ggcataacag ccttgtctgc ttatctactg
    10261 cttattcagc cactaaatac tcagatagat actgtctgga caggtgctga agggatacta
    10321 ggagcagtta caactgggct catcatcgga tggggctatg cacgtttagt aagacttgcg
    10381 aaagggtgga agcaaagaga gaagatacct gaaaatgttt ggcagtcttt tacttcattg
    10441 attccaatca ttgttatagt cacttttacg cttatcggtt cagcggtttt tcaacatttg
    10501 ttcagaatga ctgttgttca aggagtatgg acatatgtcc agcttcccat tcaatcgttc
    10561 atagacacgt tgtttggtgt gcttttatta ggtttttttg ttccgttttt atggttattt
    10621 ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc ccgtcttgca agcgaattca
    10681 ttagcaaatg cagaaatctt agcctcagga caagagttga cggtggcaaa tggcggacat
    10741 atcgttactc agcaatttct cgatcagttc atgacggtta cgggtgctgg attgacgtta
    10801 ggggctgtat tttttatgac ggtttttgcg aaatcgaaga aatacagaga attaggaaat
    10861 cttgctcttt taccagcctt tttcaacatc aatgaatcaa ttattttttc cacaccgatc
    10921 gtgatgaacc cgatgatggc tgttcctttt attttcgcac cggtcgtttc aggattgatt
    10981 acctattccg ctttatattt tggattcgtt cctttattta cagcagtcca agtcccttgg
    11041 acaacaccgc cgatcctctc gggtttctta gtaggcggag tgcctgcagc gattttacaa
    11101 ggtatcgtgt tgtcaattag ttttttcatt tatttcccat ttttgaaaaa aatagattct
    11161 gcaaactgca aaaaggaaag acagacaaaa aatgatggag cagcagaaaa gatgaacgac
    11221 taaaaaacaa aaatactcct gtcgctttag attgggaaaa tcaccaatct aggatgacag
    11281 gagtatttat agtcatgttt atactagccc tttttggaca gctaattgaa caagaacaac
    11341 taatgtgttc attcctacgt gtgtgatcat cgatgtccag atccgaccag ttgctttgta
    11401 ctctaggctg aagaagaaac ctaggaaaaa gtagattagc agatgaccat cattatgagc
    11461 aaacgcaaac agcaaagagc tgatcactgc tccgacccaa aaatttgaat atcgactgat
    11521 gatccccagt atactgcggc ggaatacgaa ctcttccatg atgggtccag caacagttgt
    11581 cgccaaaata aaagcgggtg cttccatgat catctggatg atattttgtg tattctcgga
    11641 aggggttgct ttgccaaaca atacagattc taacatgaca gcgatgcttt gcagaatcaa
    11701 tgcgacaaag attccaacca atccataaat aacaatcttt ggagcagaag ctttttttac
    11761 cgctgattcg atcgctaagg gttcacgttg cttaaaataa aaaatagcta ggacaacggc
    11821 gcctagtata tacatgactg cagttgctgt gatcaattca gacgtagatt ttacaagtcc
    11881 tatcgaagct aggatcaagg gtgagataaa aattagacca tagcaaaaaa tgctgaggaa
    11941 gctatatttt tttaaagaca tgtaattcct ccttttaagg gataacaagc atgcaaggca
    12001 agcttatatc gattgatttc aattatatca taatttcaat aagcagctgt cagaataaaa
    12061 tttcttttct ttgatttaaa agcttgcaaa aaagagggag aatgagtatt attaaaaatg
    12121 taggttagca ctcgaaggtt aagagtgcta acagattaaa aaaagagtta tcggagggat
    12181 ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga agtcgcaaaa gaagaagaaa
    12241 aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga gaaaccccaa actgggacag
    12301 ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga aaaagttcct gccgctgtaa
    12361 aagcaggcga ccaagttatg ttcgaaaaat acgccggtac agaagtaaaa tacgaaggaa
    12421 aagaatatct gatcgtcgca ggaaaagata tcatggcgat tgttgagtaa atttggaaga
    12481 taaaaaaata atcaaaaata atcaatgagg tgaatcaatc atggcaaaag aattgaaatt
    12541 tgcagaagac gcacgcgcag ctatgttacg cggggtagat aaattagcag atacagtgaa
    12601 agtaacatta ggacctaaag gccgaaatgt cgtactagaa aaatcttacg gctctccatt
    12661 gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa ttagaagatc attttgaaaa
    12721 catgggtgca aaacttgtat cagaagtagc atcaaaaaca aatgatattg caggagacgg
    12781 tacaacgaca gcaactgttc tgacacaagc aatcgttcgc gaaggtttaa aaaacgtaac
    12841 tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa ttggcaacaa aagcagcagt
    12901 cgaagaattg cataatatct caactgttgt tgattcaaaa gaagccattg cacaagtagc
    12961 agctgtttct tcgggttctg ataaagttgg acatttgatt gctgatgcaa tggaaaaagt
    13021 tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg atcgaaacag aactagatgt
    13081 tgtagaaggt atgcagtttg atcgtggtta cttatcacaa tatatggtaa cagataacga
    13141 caaaatggaa gctgttctag aaaacccata tatcttgatc acagacaaaa aaatctctaa
    13201 catccaagat attctgccat tgttagaaca aatcttgcaa caatcacgtc cattgttgat
    13261 tattgctgac gatgtagatg gtgaagcatt gccaactctt gtattgaaca aaatccgtgg
    13321 aacattcaac gtagtagctg taaaagctcc tggtttcggc gatcgtcgta aagcaatgct
    13381 tgaagatatc gctattttga caggtggtac agtaattact gacgatctag gtttagaatt
    13441 gaaagatgca acaatcgaaa acttaggaaa tgcttctaaa gttgttgtag ataaagacaa
    13501 tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc gaagctcgtg tgcaattgat
    13561 caaaaaccaa attgctgaaa caacttctga ttttgaccgc gaaaaattac aagaacgctt
    13621 agctaaatta gcaggtggtg tagctgttgt taaagttggt gcagctactg aaacagaatt
    13681 gaaagaatta aaattacgca ttgaagatgc attgaatgct acacgtgccg cagtagaaga
    13741 aggaatggtc tcaggtggtg gtacagctct agtgaacgtt atcagcaaag tttctgctgt
    13801 agaagcagaa ggagacgtgg caacaggtat caagatcgtt gttcgtgctt tagaagaacc
    13861 aattcgtcaa atcgctgaaa acgctggtta tgaaggatca gtgattgttg ataaattgaa
    13921 gaacgtggaa ctaggcactg gtttcaatgc tgctactgga gaatgggtaa acatggttga
    13981 agcaggtatc gtagacccaa ctaaagtaac acgttctgcc ttgcaaaatg ccgcttccgt
    14041 ttctgccttg ttattaacga cagaagcagt cgttgcagat aaaccagaac cagcagcgcc
    14101 agcagctcct gcaatggatc catcaatgat gggcggtatg atgtagtaat taagaaagaa
    14161 ccctatttta acagggttct ttcttctttt tttgttttat tttaagcaaa aggggcaaag
    14221 aaggggcaaa aatatcaaag gctatttagt ttatcaatta cttgttgttt ggctcttttt
    14281 gttacatggt tataaataga cagagttgtg tttgcatcag aatgccctac acgctccata
    14341 atggctttaa gaggtacgcc taattcagat aatagtgaaa cgtggctatg tctgaatata
    14401 tgtgaggata gacttttttc taattccaac tcttcctcta ctttatggag tatagcgtta
    14461 aatgagtgca gcgcaagcgg agtgccactt gtagatataa atatatattg atcgggatct
    14521 gtgggtctac ctgcaagaat attgtcagct atcacacttt caatcaattc ttttgcacga
    14581 ttgggtaatt gcacttcacg ttgcgaataa gtattttttg gagttgtttt tatagcatta
    14641 tccattttca cagatgtgta atctaaggtc ccattaatgg aaattttccc atcctcatag
    14701 tccttcattt gcaaagctag caattcccca tatctcaaac cagttaaata tagaaactca
    14761 gctattatgc cgtgtagttt tctgcgagga ttggagtata gctgtttcag tatttgatca
    14821 atttcttctt tctccagata ttttttattc atagaaagcc ttcttttttc ttcttccact
    14881 tttttaggat ggattttaac tgctagcgca gggtttcttt gaatgtattt tctatcgatt
    14941 gcatagttta gcataacaga tagagttgtt tttgtttgtt ttgtgtagtt cagtgagagg
    15001 tcaccaaacg tatacatatc ttcaattatc ttattaatga gtgtctcatc aatgtttcta
    15061 acgatcgtat catcgcttat gtgcttagaa acatgtttca tcatcattgg aaccttcaaa
    15121 tagctagtac gtttaacatg ctgcttataa tattcatacc attctttata cagctcacca
    15181 aaagtgatat ctgatttatt gtaatcttcg agtgcttctt taattttttt atctagaatt
    15241 ttctgagctt ttttccacgc ttgtggtgaa ttacttgtaa gtgttgtaga tttttttcgt
    15301 gttttttctg tataaggatc tacatatctt tcaataaact tgaattggcc atctttggtc
    15361 tgttcaaccc acacttttaa catctcctat catttgctat aataggcata acaaatagac
    15421 ctatataggt ttgttttcta aaagcacgct cttactctgg acggtggggc gtgtttttta
    15481 tttattatac agttgcttta aaaatagctg ctttttcaaa tgatactaaa ggagagaagt
    15541 ggatttctat ttctcccttt tggtttaacg tgaaatgagc agttacatcc atttgttttc
    15601 ctggagcaac agatcccatt gtattttcgt tggcatatgt ctctgatttt ttgtcatctg
    15661 gtccatatac ttccacatct gtacctacag ggatatctga atcgccatca ttttttacgg
    15721 tataagtaat ttttactact tgtgcaggtt ggttttcttc aaattgattt ctttcatcag
    15781 ttagttctac actatttagt gtatattcag catcaccaac ttttacagtg tcaccaatct
    15841 tatagaaggt atcgcttttt tcttctttag aagatgaagc agaggttgaa gatttagtta
    15901 ctttttcgcc accattatca cttgctttat tactatctga tccaccattt aaagcagaac
    15961 caataattat aattaaaatt actgctaata cccaaaacca tacttttttg taaaaaggtt
    16021 tactaacttt atacattttt ccgtcttgac ccataacttt ttttgccatt taaatattcc
    16081 tcacttcttg ttataatata tttgcgatct cagaaatgag gtatgagtcc gtgtgccagc
    16141 acgggctttt ttaatgtttt gctgaaatcg gtttttttaa taattcttga tgtattcata
    16201 catattgcct tgcgtaagaa tattttttct taaaatagca ttggcagaca gcataacaag
    16261 tgtatctgag cttattctga atagaataat actccataaa tttttcgaga ttaaactgag
    16321 attcatcagt cagttcgttc tcaatataga tatttaataa aattagaata gctattttat
    16381 ctgcttctgt ttcaaatttt gagtgaaaag ttgtagaagt atcgtacaaa actgaatatt
    16441 caaaatgaga agcggtgaaa tgcgcaagtt catgagataa atgaaaggct tctgcagttt
    16501 cgccatatag attttcattc aaaaaaatga ttctaggctt cggataatag aagccaggct
    16561 ctttcatttc catgtaaact actttcaaat tgtattcgct caacatttct ttcaatttca
    16621 aatacataca acccatcact ccaactattc attttcctct aaagctttag caattgcaat
    16681 cgctttacgc attgtctcct tagatatttc ttttccatca aaagaaaaaa cagtatcgtt
    16741 ttctgataaa tccacatgtt taggggtttc tctttcttct ctacctagaa ggtagtctac
    16801 agagactcca aagtaatcag ctaaccgagc taactcaata gaattaggag tttgtctttt
    16861 ccaacttcct aaatatccat tcgaataccc gaaattcatt tcgagctgtc taatggataa
    16921 tcctttttct tttgctaact cctttattat ttcgtaagta ttcattgata aatcaacctt
    16981 tctgaatgct tacaaaaaaa gtttagataa ataatgtaaa aatagttgac ttatatacat
    17041 tattaatcta tactatgttt tgtaaacaag tttaacaact aaaaagacaa caaaaaatac
    17101 tattgattaa taaatgccaa ccgccaagaa agctttttaa atcaaagttt atatgtctta
    17161 tttaactatg catttagtat agattattaa tctaataaag tcaacgcagt tttttaaaaa
    17221 gttgttaaat ttgtttacga atataaaaga aaggagagaa gaatatggaa aaaacagtct
    17281 cggcaaaaat cgaagatttg aaattagaca tactgaaaca agcaaaagtg gcgatggaac
    17341 acgcggtaga taaagaagac tctgccatgg ttgcagccat agcagagatt ttagctcacg
    17401 tttagtcatc gtttttacgg tcttctggaa gctgaccata gataacagca taatgaatat
    17461 ctaaataagc ttgaacaatt tcttttggcg aaattgattc gcctttagta gatacttgtg
    17521 attcgtgatt tagccatgca acaactaaat ccgcagcgat ttcaaacggc gttacacttt
    17581 gtgtcagttg ccataaagaa tttcacaata tgtatggtcg tggtaataat accaaagagc
    17641 aatttgagga atttatggaa attaaaaaat acaaaaaatc atttattaaa ggaggctaaa
    17701 caatgaacaa ggaaatgagc caaacaatca aggtacaaaa aatgatagat gatctaacac
    17761 atggaattga tagccaagcc gataaaatca tcaaggaatt gcaaggacaa aaagttaagg
    17821 atgcaaaaat gcttctcaag actatcaact ttgaaatgaa tccaacaaaa agaaagctcg
    17881 ccgatgtgtt ggaagaaaag ttggcttccg ctatcaacga gcaagaatta ctatttgaaa
    17941 ccgatacttt taacagctga tgttttatag atgacagttt catccgaatc tatatcatcg
    18001 ccgagagaaa agaatgttgc ttttgacaaa aagccgataa gacctacaag cggtcttgat
    18061 gttgcaagag tagatacccc aatatcacct tgcaaggtat tcacgtaaat ttgagaagaa
    18121 tagaacattc catctttttc ttcgatattt gacgcatcat cgtatccaac gaccaaagta
    18181 tcggcaaata ttttgatttt ttctccatca agcatagtaa ttacaaattc tggtttcata
    18241 aattttcacc tccttaacaa ttatttcagc ctgtcacact gataaggaaa ttataccaaa
    18301 gaaaggaatg agaaatatga atacaccaca aatttttaat ttcgagcaaa acgaagttcg
    18361 aactatttta gtaaatgatg aaccatattt tgtaggaaaa gatgtagcag acgttctagg
    18421 atattcgaat ccgcaaaaag ctattcgaga tcatgtggac ttggaggata agacgcagaa
    18481 cgattcgttc accgtcaatg gaacagcagt tgttttgatt aacgagtcag gcctttacag
    18541 tttaatctta aaatcaaaac ttccctctgc caaaaaattt aaacgttggg taacgagtga
    18601 agtccttcca acaattagaa aaacaggtag ctattcaaac gtacctcaaa gttttgcaca
    18661 agcattgcgt ttagcagcgg atttagaaga aaagaaccaa ttactcgaac aacaaattgc
    18721 cgagtacgaa ccaaagatta gctacttaga tacgattctt tcatcgacag atacggtagc
    18781 tacttctcaa attgcagctg attacggaat gtcggcaatt gctctaaaca aattgctaaa
    18841 cgagttaggt gttcagcata aagttagcgg acaatggata ctttaccgaa aacatatgaa
    18901 ccaaggatac acaaaatcgc acacaagtga gataccgaaa gccgatggcg gcactaaagt
    18961 tgtaatgaat accaaatgga cacagaaagg gcgagtgttt atttacaact tattaatcgc
    19021 agagggctat taccctcaaa tggatttaga ggaaattggt tagaaaggag ttttagtatg
    19081 actgacattg cagaaatcac tcaacgagat agagaaaaaa tcaaagaata tgtcgaaagt
    19141 tcaaagttct taacttacac catgcttgct gaaagatttg gaattagcaa aagctactta
    19201 tctttaattt taaacggtaa aaagacttct gcagaagcaa acagaattat agattcgatt
    19261 atcactatgt acgaattgta gagggaggta aagtcgttgg aagataaaat cattcttaca
    19321 aaatcagagc ttcaagaaat gttaaacatc gagtatggaa cgataatttt taggagggat
    19381 aataagtgaa aaaaccaaca ctttcagagc tgatagaagc tgctgagaag gcagtaaaac
    19441 cagacgactg gtaccgacaa agtttaatct tggagaagtt ccacggcatg tcaaaaacta
    19501 ctttagttga atactgcaag gaaatggaaa caattcctga atttgcagaa ggaatacttc
    19561 gtccaggtca ctcaaccaca tttattcatt accatacttt tatttggttt ttaaagtgga
    19621 aagacgcaaa caaatatcgt gtaaaaagtt tatcgccttc agatgttttg aaggaggcaa
    19681 gttaatgggc aaattcaaca gagcactagt attcagcgca ccgctaatca tctacgcttt
    19741 aggcctctgg ggaagcagac aagcgttgat agggacaatc gtttacatgg tttggatttt
    19801 tatggggctt gatgaagctg agcacagagc gaaaaaagcc agtcgggagg gactgactaa
    19861 aatatgattt ttttattttt gttcctttgg gctgctattg cgtacttcat ccagctcctt
    19921 gttgtcaaag ttgctcttat ttcttttagg aagaacaaaa gaaaatagcg ctataaatga
    19981 aagataagta gccgctacat taaactgttc ttttgaaaaa gttacagtca tcggaatgat
    20041 aattgatatt gaggaagtaa tcttaattaa aaatatagca atattttcaa catcgcgttg
    20101 gtttttattt aacattgtcc aacatgtttt agcaacaata aaaataatta cacatgatat
    20161 tagcacacag actgaaaaaa gttttagaaa tgcgatgtag tcagtgttat tgaactcctt
    20221 caaaatacta tttttattca atgtaataaa aggaattttt ttaattgaag acaggaaaga
    20281 taaggtaata aacagaatta tatatatggt taaagtcgca gtaagaatgt aattgagctt
    20341 tttcctcata gaatcacctc actttctaac gaaaattata ccaaaaagga gagaagaaat
    20401 aatgcaagaa ttagtaattt tgaaaaataa agaagctgta actacgagct tacaagtggc
    20461 agaaagtttc aaaaagaaac ataagcatgt actagaagca attgaatcaa taaaaagatc
    20521 ggtcgaaaat tcggccaatg ttgaagatgg gtccaatttt ggacagatgt ttgtggaagg
    20581 gaacgagcca gactcatacg gaagaagtcg gagagtttat ttcatgaata gagacggatt
    20641 ttccttgcta gctatgggat tcactggaag taaagcaata aatttcaaac taaaatttat
    20701 tgaagctttc aacgaaatgg aagatgttat tcggaagaat actgttcctc aaacaattga
    20761 agacatgatg atctatcaat tagaagaaat gaaagatgtt aaaaaagatg tttccatgct
    20821 taaagatact atgcgaatta gcggacaaca agagtttgaa attaagcaaa aaggaaatat
    20881 gaaagttatg gaagttctag ggggaaaaga aagccgagct tatgaagaaa tcagcaaaaa
    20941 agtattctca aaattttggt ctgaatttaa acgtaccttt tcaatcccaa gatatggcga
    21001 gttacctcgt aagagattcg atgatgctgt ttcatttatt gaaatgtggt taccagaaac
    21061 tgcgatccgc atggaaatcg atcaactgaa cagacaacaa agacttttcg gtgatgaaaa
    21121 tgaatagagc tgaagcgcta agaataggga cggtaattgc taatcgctgg tggagacaca
    21181 ataaaccaaa catcctaagc caacaacata tcgaaaagca aaaagcatgg caacaaataa
    21241 aaaagtgact cagccggcaa gcatagagtc acaaaacata tctaaggaga attttagcat
    21301 atgaataaag aactttccac tttagatcaa tatttgactg attctgaatg gggcaagtcg
    21361 aatatcaagg aaacaaataa tcgaaaaatc agacgaaatc ttttgacgaa cgaagaacta
    21421 tcatgtgatc aagatgactt gggaaatttt gtgagtattt gggatcatgt ttaccttatc
    21481 catctatcaa aacattcaaa taaacctgaa tacatctatg tcatcgaaga tggcttgatt
    21541 gatgcgctag aagagtacaa cagagataac ttgattgata tctcttatta cggatcaggt
    21601 aagaaataca ttgctgaaat ggaggcagaa tttgatgagt gaaatcaaag ggacaacgaa
    21661 ctttgaaaaa ctttttagtc gtaagttaaa taaaattctc aagaaaaaag gaaattttga
    21721 ttatttatct tgggctcacg cgtgggagat tatgaaaaag aatgatccac aggcaacggt
    21781 aactattaat gagtataaac actacagggt tgtttctgga actcatcaag actttcttgt
    21841 tgaggaatat aaaccttttc ttatggatga aacagggact tatgtatctg tctcagtaac
    21901 ggttaaagga catacggaaa caga
//
LOCUS       DANKAZ010000041        20873 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000041 DANKAZ010000000
VERSION     DANKAZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            99..293
                     /locus_tag="PWY67_001492"
     CDS             99..293
                     /locus_tag="PWY67_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057787.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="PWY67_001493"
     CDS             562..861
                     /locus_tag="PWY67_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057788.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="PWY67_001494"
     CDS             complement(958..1392)
                     /locus_tag="PWY67_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2057789.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="PWY67_001495"
     CDS             complement(1624..2334)
                     /locus_tag="PWY67_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2057790.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="PWY67_001496"
     CDS             complement(2324..3184)
                     /locus_tag="PWY67_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL2057791.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="PWY67_001497"
     CDS             complement(3189..3827)
                     /locus_tag="PWY67_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL2057792.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="PWY67_001498"
     CDS             complement(3842..4141)
                     /locus_tag="PWY67_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2057793.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="PWY67_001499"
     CDS             complement(4184..5668)
                     /locus_tag="PWY67_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2057794.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="PWY67_001500"
     CDS             complement(5688..6149)
                     /locus_tag="PWY67_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2057795.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="PWY67_001501"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="PWY67_001501"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL2057796.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="PWY67_001502"
     CDS             7452..8222
                     /locus_tag="PWY67_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2057797.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="PWY67_001503"
     CDS             complement(8495..9322)
                     /locus_tag="PWY67_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2057798.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="PWY67_001504"
     CDS             9890..11125
                     /locus_tag="PWY67_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2057799.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="PWY67_001505"
     CDS             complement(12124..12384)
                     /locus_tag="PWY67_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2057800.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="PWY67_001506"
     CDS             complement(12411..13712)
                     /locus_tag="PWY67_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2057801.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="PWY67_001507"
     CDS             complement(14004..14462)
                     /locus_tag="PWY67_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2057802.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15806)
                     /locus_tag="PWY67_001508"
     CDS             complement(14475..15806)
                     /locus_tag="PWY67_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL2057803.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17259)
                     /locus_tag="PWY67_001509"
     CDS             complement(15817..17259)
                     /locus_tag="PWY67_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2057804.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="PWY67_001510"
     CDS             complement(17246..18010)
                     /locus_tag="PWY67_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL2057805.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="PWY67_001511"
     CDS             complement(18114..18989)
                     /locus_tag="PWY67_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2057806.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19244..19328)
                     /locus_tag="PWY67_001512"
     tRNA            complement(19244..19328)
                     /locus_tag="PWY67_001512"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19292..19294),aa:Leu,seq:caa)
     gene            complement(19346..19417)
                     /locus_tag="PWY67_001513"
     tRNA            complement(19346..19417)
                     /locus_tag="PWY67_001513"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19383..19385),aa:Gln,seq:ttg)
     gene            complement(19427..19499)
                     /locus_tag="PWY67_001514"
     tRNA            complement(19427..19499)
                     /locus_tag="PWY67_001514"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19464..19466),aa:His,seq:gtg)
     gene            complement(19561..19645)
                     /locus_tag="PWY67_001515"
     tRNA            complement(19561..19645)
                     /locus_tag="PWY67_001515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19609..19611),aa:Leu,seq:caa)
     gene            complement(19666..19736)
                     /locus_tag="PWY67_001516"
     tRNA            complement(19666..19736)
                     /locus_tag="PWY67_001516"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19702..19704),aa:Cys,seq:gca)
     gene            complement(19782..19853)
                     /locus_tag="PWY67_001517"
     tRNA            complement(19782..19853)
                     /locus_tag="PWY67_001517"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19819..19821),aa:Gln,seq:ttg)
     gene            complement(19865..19937)
                     /locus_tag="PWY67_001518"
     tRNA            complement(19865..19937)
                     /locus_tag="PWY67_001518"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19902..19904),aa:His,seq:gtg)
     gene            complement(19940..20013)
                     /locus_tag="PWY67_001519"
     tRNA            complement(19940..20013)
                     /locus_tag="PWY67_001519"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19979..19981),aa:Trp,seq:cca)
     gene            complement(20020..20100)
                     /locus_tag="PWY67_001520"
     tRNA            complement(20020..20100)
                     /locus_tag="PWY67_001520"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20064..20066),aa:Tyr,seq:gta)
     gene            complement(20102..20177)
                     /locus_tag="PWY67_001521"
     tRNA            complement(20102..20177)
                     /locus_tag="PWY67_001521"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20142..20144),aa:Phe,seq:gaa)
     gene            complement(20213..20285)
                     /locus_tag="PWY67_001522"
     tRNA            complement(20213..20285)
                     /locus_tag="PWY67_001522"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20250..20252),aa:Asp,seq:gtc)
     gene            complement(20295..20367)
                     /locus_tag="PWY67_001523"
     tRNA            complement(20295..20367)
                     /locus_tag="PWY67_001523"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20332..20334),aa:Val,seq:tac)
     gene            complement(20383..20454)
                     /locus_tag="PWY67_001524"
     tRNA            complement(20383..20454)
                     /locus_tag="PWY67_001524"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20419..20421),aa:Glu,seq:ttc)
     gene            complement(20469..20558)
                     /locus_tag="PWY67_001525"
     tRNA            complement(20469..20558)
                     /locus_tag="PWY67_001525"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20522..20524),aa:Ser,seq:gga)
     gene            complement(20598..20671)
                     /locus_tag="PWY67_001526"
     tRNA            complement(20598..20671)
                     /locus_tag="PWY67_001526"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20635..20637),aa:Asn,seq:gtt)
     gene            complement(20681..20796)
                     /gene="rrf"
                     /locus_tag="PWY67_001527"
     rRNA            complement(20681..20796)
                     /gene="rrf"
                     /locus_tag="PWY67_001527"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6573 a         4383 c         3325 g         6592 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
    19261 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
    19321 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
    19381 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
    19441 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
    19501 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
    19561 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
    19621 cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat
    19681 ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta
    19741 tactattatc aatctataaa ataaagaata aattatagaa actggggtag ctggattcga
    19801 accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa
    19861 aaagggcgaa tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac
    19921 cacttcgcca cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg
    19981 gagaccgctg ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac
    20041 cactgaaccc taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc
    20101 atggtggcgc gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc
    20161 aactgagcta ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg
    20221 acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg
    20281 gaaccagttc attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag
    20341 atgctctccc agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg
    20401 aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa
    20461 atttgctacg gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat
    20521 ttccaatcat gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact
    20581 ctttctcaaa atgagaactc cggcagtagg actcgaacct acgacatcat gattaacagt
    20641 catgcgctac taccaactga gctatgccgg aataacatca gcgtggcgac gtcctactct
    20701 cacaaggggc aacccctcac tacaatcggc gctaagaagc ttaacttctg tgttcggcat
    20761 ggttacaggt gtatccttct cgctatcgcc accacactgt ggtgttatct tttattgagt
    20821 aaattttgtt cactcaaaac tggatttgaa gtcacataag tttttttccg agt
//
LOCUS       DANKAZ010000042        20462 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000042 DANKAZ010000000
VERSION     DANKAZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            22..1101
                     /locus_tag="PWY67_001528"
     CDS             22..1101
                     /locus_tag="PWY67_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2057807.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(1298..1999)
                     /locus_tag="PWY67_001529"
                     /pseudo
     CDS             complement(1298..1999)
                     /locus_tag="PWY67_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2013..2937)
                     /locus_tag="PWY67_001530"
                     /pseudo
     CDS             complement(2013..2937)
                     /locus_tag="PWY67_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3054..4133
                     /locus_tag="PWY67_001531"
     CDS             3054..4133
                     /locus_tag="PWY67_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2057808.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            4165..4758
                     /locus_tag="PWY67_001532"
     CDS             4165..4758
                     /locus_tag="PWY67_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2057809.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(4868..5095)
                     /locus_tag="PWY67_001533"
     CDS             complement(4868..5095)
                     /locus_tag="PWY67_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2057810.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(5198..7786)
                     /locus_tag="PWY67_001534"
     CDS             complement(5198..7786)
                     /locus_tag="PWY67_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL2057811.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(7806..8465)
                     /locus_tag="PWY67_001535"
     CDS             complement(7806..8465)
                     /locus_tag="PWY67_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2057812.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(8514..9017)
                     /gene="trmL"
                     /locus_tag="PWY67_001536"
     CDS             complement(8514..9017)
                     /gene="trmL"
                     /locus_tag="PWY67_001536"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL2057813.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(9238..10104)
                     /locus_tag="PWY67_001537"
     CDS             complement(9238..10104)
                     /locus_tag="PWY67_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2057814.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            10213..10854
                     /locus_tag="PWY67_001538"
     CDS             10213..10854
                     /locus_tag="PWY67_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL2057815.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(10916..12277)
                     /gene="mgtE"
                     /locus_tag="PWY67_001539"
     CDS             complement(10916..12277)
                     /gene="mgtE"
                     /locus_tag="PWY67_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL2057816.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(12295..13194)
                     /locus_tag="PWY67_001540"
     CDS             complement(12295..13194)
                     /locus_tag="PWY67_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2057817.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(13196..13993)
                     /locus_tag="PWY67_001541"
     CDS             complement(13196..13993)
                     /locus_tag="PWY67_001541"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL2057818.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(13971..14675)
                     /locus_tag="PWY67_001542"
     CDS             complement(13971..14675)
                     /locus_tag="PWY67_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL2057819.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            14806..15381
                     /locus_tag="PWY67_001543"
     CDS             14806..15381
                     /locus_tag="PWY67_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL2057820.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            15499..16176
                     /locus_tag="PWY67_001544"
     CDS             15499..16176
                     /locus_tag="PWY67_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2057821.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(16255..18063)
                     /gene="pepF"
                     /locus_tag="PWY67_001545"
     CDS             complement(16255..18063)
                     /gene="pepF"
                     /locus_tag="PWY67_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL2057822.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(18239..19237)
                     /locus_tag="PWY67_001546"
     CDS             complement(18239..19237)
                     /locus_tag="PWY67_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL2057823.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(19463..20116)
                     /locus_tag="PWY67_001547"
     CDS             complement(19463..20116)
                     /locus_tag="PWY67_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL2057824.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5920 a         4449 c         3647 g         6446 t
ORIGIN      
        1 aatagacaag gatgaattca catgacgcaa acccatgata acacagtttc acgcaaggga
       61 aaacatcttt cctactcaga acgttgccaa atcgctatcc tgaaaaagga aggctattcc
      121 aaccgtcagg ttgccaaagt gctgggacgt gttccgcaaa ccataaacaa tgagattgat
      181 cgcggaacca tcacccagtt aaaacgccaa acgcaaaatg gaaaagtgta tgacacctac
      241 tattctgttt atgacgctga tgcaggacag gccttctatg aaaagcaacg actgaactgt
      301 ggcagacgcc ccaaatgggc gaacacaaat gccttcattg attgggcaga tgacaaaatg
      361 ttaaatgaaa aatggtcgcc ggacgtcgtg gtcggatttg ccagaaaaca cgagctgttc
      421 gattgtgcca tcattcctgg cacaaccgct ctttatcaat ggattgaccg cggaatcatg
      481 cgaacaaaga acatggactt gcttgaaaaa ctgtcccgta aaatcaaagc ggtccgccca
      541 ggacaccgtc ccaacaagcg tgttctcggg acgtcaattg aagaacgccc taaagaagtt
      601 gaaacacggg aaacgttcgg tcattgggaa atcgacacgg tagtgggaaa caagaccaag
      661 tcggatgccg tgctgctgac gctggctgaa cggcagacac gctttgaagt catcctaaag
      721 ttgaacggga aggacaccga atcggtggac caagcggttc aacagctaag agaacgggct
      781 ggcgctaact tcgatcaatt atttaagacc gttacatccg acaatggctc ggagttttct
      841 gggctacacg atgctctgca agatgtagct gacgtctatt tcagccaccc ctatgcttct
      901 tgggagcgtg ggacgagcga aaaccagcac aaattaattc gccgtttcgt ccccaaggga
      961 agcccgatta gtgaggtgag cgagaaccag attttgcgga tccagcgttg gatgaacgat
     1021 tacccgcgga aaattttggg ttatgcgaca ccacatgata cattcgtcca ggcattcaag
     1081 caagaacggt tggtggcttg acccattatt ccttgataac gatcctcact cgggacatgg
     1141 cttcatgtct ggaaagctgg cctggggcca gaatcgtctg aagactatca tacctcagtt
     1201 cattatcaag gcctcactga tttagaataa cgaggcagtt acggtatcaa acaaatgagt
     1261 tacctgtagt ggctaactta aacttgaaat ttacggacgt aaagcttgct gccaaattag
     1321 cgattgaaac taacgattgc tttctaggaa tcaaggacaa gcgaatacca tttgacgatt
     1381 gatttgcttg ctgatcctgt acattgcgga ccccccacat cgtcccgtag agaatcgcca
     1441 ttccaatcag agtgaagaaa taaaaacttt tgaaactcat attacgacct gcatgaactt
     1501 cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg cgcgagatca acgttctcca
     1561 cttctgccac tttactcgaa attataccaa tacgctgtaa aaattgatcc atgacgtttt
     1621 tcatcaacgt ctgactcatt cctgcttgac ccacatggag cgaaacagat gaaccatcaa
     1681 tgagataaaa accagccaaa tcatctgcat ttagcttttc attcgcttct acttccgata
     1741 gttcctgtac ttcaaataat ggtttatcat tgacttcgac ttgttctaag actgtggtaa
     1801 aattttccga aagcgctgtt tgatccgctt tgattacaat tccagtatta atcgtttcga
     1861 gattgccacc ttgatcaatc tcgccaaagg ccgccataaa catcaatcct aaaagaattg
     1921 gaaaggccaa tgtccaaaac aacatggatt tatttcgcag cagtattttt gtgcgatatt
     1981 tataaaaatg ccaaaacatt atttcccctc ctctagtctc tcaaatcctt gccagtaatc
     2041 tctaaaaaga catcattcaa ggacgcttct ttggttgcta tttttccgta actgatcttt
     2101 tcttcttgta agattgtaag aatcggcaac aatactgtat ttagttcagt agttgttact
     2161 tggagctgac cttttttata agcgattcca tcagaagcta ttgttcgctg acaacacgtt
     2221 ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca tatcttttag
     2281 agattctttg gttcctttag caatcacctg tccttgatcc atgatcacga tctggtcgca
     2341 aagaatttct acttcttcca tgtaatgtgt tgtatagaca atggttgctc cttgtcgatt
     2401 cagctccttg atactttcta aaattttatt ccgactttgg gggtctacag ctactgtcgg
     2461 ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca catgcgatgt tcaaccgacg
     2521 tttcaagcca ccactaagtt ctcctggacg aaacgaaaca tatttttcta atcccactaa
     2581 ttgaatcacc tcttcaatca aggctttcct tttctctttt tcggaaacat ataaaccgca
     2641 aaaataatcg atattttctt gcacacttag ttcgtcaaag accgcaacgt cttgaggaac
     2701 gaccccaatc attgccttga cagcataatt ttcaggagtc attgttgtcc cgaacaattt
     2761 aatggttcct ttgtcataat ttaacaaaga aagcatacag ttgatggcag tcgatttccc
     2821 actgccgttt ggtcctaaca gacctaagac ttctccttct tgtacttgca gatcaaaatg
     2881 attcaatgct acaaaatctc catagcgttt gactaattta tcaatttcga taatcatcat
     2941 gaaaaactcc ttccaaattt cttgttgatc tgactataat aaagtgcaag aaaagaaact
     3001 agtgacgatt ggctaaacca cacatgacat ttgtcacaat gaaggagtct gctatgcata
     3061 aatctcggct actcaacgaa actagctttc tactggttat ctcattgctt ttgctggtaa
     3121 gtcaaggatt cgttgctatg caagttatct atttattgat cgccgtttta ttcactggtg
     3181 tcagttattt actgcctaca aagccaaatt ggattttcca agttattcta tttcttttat
     3241 gtgccttttt acctgatctt gtcctgtttc tgccagcgac gatgcgagtg atcgcccctg
     3301 aagaaagcca ccttgattac ttgtttttgt taggtttttt gttgatttcg acggttggta
     3361 atcatccccc catcaaggtc gcctttgtcg taattttgct gtttttttcc ttttacttac
     3421 gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta cattgtctcc aaagatactc
     3481 attgggaaaa acaacaagag ttatcggaac aaaataaagc attggccctg tcacaagaaa
     3541 cacaattagc cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac atccatgaca
     3601 atgtcggtca tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa atgatcaatc
     3661 aagaaccaaa actaaaagaa ccattagaaa atttaagtgc caccgttcac actgggatgg
     3721 atcggattcg tcaaagtgtc catgacctcc atgagacatc gatctctttt caacactcct
     3781 tagagtttct gatcgctgat tttccttctt ctgttacgat tgaaggaaac ttgtttgaag
     3841 gattgaatga aacgcagcaa aactgcttct taatggtcat caaagaagcc ttaaccaaca
     3901 gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg ccggcctttt
     3961 atcgcttgca aatcacgaat gatggcacca gcccacaaaa aaattctgct cccggtattg
     4021 gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat attttccaag
     4081 aaaaccagca atttcagtta acgatcattt taccaaggga gggacaccaa tgataacagt
     4141 agtcattatt gagatgatcc atttgtgacg aaatcgttac aaaccatttt agaaagcacg
     4201 aaagagatcc gtgtgttagg cattggtcat tgtgccaagg atgcgctagt cttatacgaa
     4261 acccatcgtc ccgatgtttt gctgacggac atccgcatgc ctaaacaaac gggcatcgat
     4321 gctgccaaag aaattttggc taaatttccc aaagccatcc ttttgctttt gacgacattc
     4381 aaagatgaag aatacatccg cgaagcattt tccatcggtg tcaaagggta tttaatcaaa
     4441 cagaacttgc aagcgattat tccttctgtc aaatccgctt ataatggaca ggccgtattt
     4501 ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga ataaaccgac gattgatagt
     4561 catgcttatg cttcttttag tgaacgtgag cttgcaatca tcaaagaagt agctgccggg
     4621 aaaaacaata aagaaattgc agatgccttg tacttgagtg acggcactgt tcgcaattac
     4681 atcagccaat tactagaaaa gctagattta cgggatcgca cgcaattggc tatctatttt
     4741 tatcagcatt tgcaataagt cttatctaac acaaaaaaac ctgaatgatg ctccttcgca
     4801 tcattcaggt ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt
     4861 gaaagaatca aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg
     4921 gaaaaatagg cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt
     4981 ctacatcagg actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt
     5041 cattgccaga ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt
     5101 aacgcccccc ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta
     5161 accaacgatc atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa
     5221 aatcgtaggg agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca
     5281 cttctgctgt aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt
     5341 ctccaacaaa tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt
     5401 cattcgtccc actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg
     5461 tatagttccc atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt
     5521 tggtccggag agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca
     5581 tccggcttgc tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa
     5641 tcaacagttc cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct
     5701 gatattgatg gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga
     5761 ttgagcaaca ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat
     5821 ttgcatcaaa ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga
     5881 tacccgtgat ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt
     5941 ggaggacttt gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag
     6001 gtttccctgg attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc
     6061 cccgatacat tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt
     6121 tttccacaat tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac
     6181 gatctttttg attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa
     6241 gaggaataat actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa
     6301 tttgttcgat ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat
     6361 ctttgtctcc tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag
     6421 ccagccatgt atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt
     6481 tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac
     6541 tggcaggcag acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa
     6601 gtagaatagg aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact
     6661 ctgcaaaaag attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg
     6721 ttaaaataaa tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat
     6781 actgtatgcc gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat
     6841 aatggatttc ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag
     6901 aaaagtacaa actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa
     6961 caagttggat aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt
     7021 ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt
     7081 gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg
     7141 ctgcaatacc gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt
     7201 cttcgactag ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct
     7261 ggtagatact gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt
     7321 gatccatccc gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca
     7381 aaccggagat ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc
     7441 ccagcaattc tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg
     7501 aaagatacgc tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt
     7561 taagctgcct tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat
     7621 tttcttgaat atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg
     7681 tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa
     7741 tcgcggcaac tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc
     7801 ttcttctata gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat
     7861 actttcagtt catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta
     7921 ctattgaaca atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga
     7981 atggctgcag ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgttgg
     8041 gcaaccgcat ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg
     8101 tgaaaaggag aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga
     8161 tatttctggt acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt
     8221 agcttatctg tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct
     8281 gtatcacgtg ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc
     8341 tttttcccaa atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg
     8401 ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt
     8461 tccatacgca gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc
     8521 agcttgtcat tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt
     8581 gcttcataca ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca
     8641 ttcataggga tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg
     8701 gttgtttctt ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta
     8761 tatactttat gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa
     8821 aaagcttcta aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc
     8881 cgttttagat gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta
     8941 ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat
     9001 aaaacgatat gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga
     9061 caatttgatt tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt
     9121 acgttagtga agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg
     9181 aaaaaacaac ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta
     9241 aaaactgcta gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat
     9301 cttcttcaaa ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa
     9361 aggtgacatg gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac
     9421 acgctgacgt tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga
     9481 ataactctgt ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt
     9541 ttcttgtggc accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg
     9601 gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa
     9661 attggtcaaa tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac
     9721 tccttctttt gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc
     9781 attcaagaag ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg
     9841 ctgcgcaata gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg
     9901 aacgaacatt ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc
     9961 ttttttggaa aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt
    10021 tacttcttct tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg
    10081 tcctcggtca atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga
    10141 tgctcacttt ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa
    10201 ggagaagatt agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca
    10261 attgctagag gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc
    10321 ccgagtactg gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta
    10381 atgacgatta ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc
    10441 atgcatactg atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt
    10501 cttactcctt caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa
    10561 gggttgcaga tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa
    10621 gcaatcgatt ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct
    10681 gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc
    10741 cgcttaatca ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa
    10801 gaattgaacg taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat
    10861 aaacaaaaaa acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca
    10921 taaaatagcc cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc
    10981 tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta
    11041 gcatcgggat taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc
    11101 ctatgacaaa ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga
    11161 tccccgtaac agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa
    11221 atccatgatc tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg
    11281 ttccagcatt accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt
    11341 cacttaccaa gtcttcatag tgactgatta aagaagcggt cgacataccc aaaaacaaca
    11401 gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt
    11461 ccacattgac cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga
    11521 tgatatcatc gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag
    11581 ctaagaagtc ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa
    11641 ctgaaagcac tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta
    11701 ggtcgcgcag agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat
    11761 agattgtttc agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt
    11821 gattggctac gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt
    11881 aatggagaag ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag
    11941 caatttgctt tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag
    12001 aaagcatatc agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt
    12061 tgtcttcttc aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga
    12121 tcagctgcct gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca
    12181 tctctaaaaa actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact
    12241 gggcaaatct ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc
    12301 ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa
    12361 ctcttctgta aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat
    12421 tcccagatcc attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga
    12481 aaaatgaact cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat
    12541 ttctgttagt cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac
    12601 cattcgcttc aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc
    12661 cgataaatct gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc
    12721 acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaaacc
    12781 agatgtatcc cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt
    12841 tttataataa gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat
    12901 agacgaaacg cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc
    12961 gatcggtgtt tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt
    13021 taccttatca tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat
    13081 ttgaccgcct tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa
    13141 aaaatatttt acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat
    13201 cctcactaat aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa
    13261 aatggatccg ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat
    13321 tcaattgatc aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt
    13381 catcatgggc aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag
    13441 caatctctgc caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt
    13501 tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt
    13561 caaacagctc gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg
    13621 ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa
    13681 catccagcaa gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt
    13741 cttccagctc gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca
    13801 agaaacgcac atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt
    13861 ctccgccgac tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt
    13921 ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga
    13981 tcgctacttt catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag
    14041 tagacatcat ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct
    14101 tcacggatac tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt
    14161 cgttcactca tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc
    14221 gttgcccaga aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca
    14281 ccgctgatca gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct
    14341 tttttatttg tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg
    14401 atctcaacaa cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata
    14461 tcagacattt cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca
    14521 atactatcga ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt
    14581 tcacggaact gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca
    14641 tatggtgcta aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca
    14701 ttcattgttt attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac
    14761 caaaaaaaga aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt
    14821 gaaatcgaat ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat
    14881 caattaacag ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa
    14941 cagctgaaga aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat
    15001 acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa
    15061 gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa
    15121 ctttctgatt tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa
    15181 cgggctgaat tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat
    15241 ctacacgatt ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt
    15301 ataaattttt taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt
    15361 atgcttgaag ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt
    15421 tacttttttc tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct
    15481 attttggggg agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac
    15541 tgtttcgaaa tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca
    15601 aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc
    15661 atgcaacaaa aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg
    15721 acttattctg cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc
    15781 cgttttttga tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa
    15841 cttgctgaat ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt
    15901 cagtctgact tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc
    15961 atcacaaaac acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt
    16021 ttagtagaag taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca
    16081 gataactcat atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat
    16141 tcaagaacta gccatttaca cttgctttct aattaaaaaa atacgtcgac tcccttacaa
    16201 gagagccgac gtattttttt cgtttctatt cgagaaaatc aaaaatagaa agccttataa
    16261 acgatcgatc aattcttcta attcatttag acgttgttca aacatcgaca ttgcatcttc
    16321 aatatatgca gcctgtgtca tatcaactcc tgcttttttc attacttcca caggataatc
    16381 gctgttacct gcttttaggt aagccaagta gttctctagt gcctctggtt cctggtttag
    16441 tattttcttc gccagtgctg aagcagcaga aaagccagtc gagtattgga aaacatagta
    16501 attataataa aaatgcggta tccgtgacca ttcgaattta atttctgggt cttcttcgac
    16561 tgctggacca taatattttg cattcaattt accataacta tcacttaggt attcactagt
    16621 caatggcaca cctttttcat cttccgtatg catgaaatgc tcgaattcag cgaattgtgt
    16681 ctggcggaaa actgttcctt taaacccatc caagtaatgg ttgagtacat aagcccgtac
    16741 acgagggtct ttttctgttt ccaataaata ctccgttagg atattttcat tcgttgtcga
    16801 agcaatttct gctaaaaaga tggagtagtc gccgtacaca taaggctggt tcgaacgagt
    16861 gaaataacta tgaacactat gtcccatttc gtggacaagc gtaaatagct gatccagcgt
    16921 atcatgccaa ttcaataaaa tatatggatt tgtgtcatag cttcccgaag aataagcacc
    16981 gcttcgtttc cctttatttt cgacaacatc gatccaacgt tcagagaatg ctttttctac
    17041 gatggccatg tattcttcac ccattggttt cagtgcttct aaagcttttt cttttgcttc
    17101 ttcgtacgta aaggtaattg gagcttcccc taagaccggt gtataaagat catacatgtg
    17161 cagtttttcc acttctaata aacgtttccg taattccatg tatcgatgta acaaaggcaa
    17221 atgcttgttt accacgtcta ccaaagtatc gtatacactt tcaggaatat gattattgct
    17281 caaagacgct tctctggcag agctgtaatt acggactttc gctttaaaat tatgtccttt
    17341 tatatgtgtg cctaaagtag aagcaaatgt atttctaaat tgttcgtaaa cactgtacaa
    17401 tcccttaaat gctgcttcgc gcacccttcg atccgtgctt tctagcaact gaccatacac
    17461 gccatgagat aattggacta tttcaccatt ttctccttca atcgttggaa aaactagatc
    17521 tgcattattc aaaacagcaa atgtatcact tgaagcatca aagatttcac ctgctccagc
    17581 aagaagagat tcctgctcag cagataagac atgggctcga ttatctacta tttgctggat
    17641 ataatggcga tagacttcca attttggttc ttctttgaaa tattgccaaa tctggtcatc
    17701 tgacaattgc aatatttctg gttcaaacca ggaaacggct tcactaactt tcgaaaacag
    17761 actgcttgct cttgcataaa gcgcttggta atctgtattt ccagtgtctt gatcgttttt
    17821 aaggtgcgca tatacataaa tgacttcagt ttggcgataa accctcaata cgaattcaat
    17881 cgcatttaaa aattgagaag cgccttgatc aagtgttcct ttgtgttttt cagattgttt
    17941 taactcttct gataattcca aatatttctc atcaaactct tggtcgcttg aaaagatctt
    18001 ggtcaagtcc caagttaaat tttcaggcaa ttcttctcgt tttggcaact gctttacttc
    18061 catgaaatcc actccttttc ttcccttagc tctatctatc aaaatagatc atagtctaac
    18121 atccacatgt cacagcatct aacttttcta ctcattcatg tgtctgcttc catcaatttt
    18181 agcataacta aatcttgttt tacatagaaa accgattcct agcctatttt tatacttact
    18241 attttttcaa agaagacaag tgtatgcatt cgttaaaaaa ctgctgatag accatctctt
    18301 catcaatcat aggaaaaagc cattcccttt tatgttcttt tatttggcaa agccatgagt
    18361 tgaaatcagg aactttctgg ttttccaagg cttcttcgta caacattcga taaagaaaaa
    18421 cctgttcttg taaataaaag aaaaaccgac ttttttgata gatccacgat gatagatgca
    18481 aaagatgttt atgacgcaag tagcattgtt cttgtatgga tactgtattc ttttgcttgt
    18541 gaataagttg gcgtgtcagc catttcttcc catcttcact cactttttct atttttttca
    18601 tttggttcat tttaatttcg ttactagtga atatttcttt aaggtcccta ctagaaaaag
    18661 tccagcagct actgcaaaag gaaatatgcc ctgatacagt ctctgtaata tgatgataca
    18721 aataaagctt ttgtttcttg agatcagctt tccagcaatg cactcctctt tttctattat
    18781 agtagcagca acttttttct atcaatgaga attgcgattg tccaagaaaa gaattaccaa
    18841 gtatccacca tacttggtaa ctgttattca aataattttg ggttctttca ataaatctct
    18901 ggtgagacag tctagaacat tggatttcta ttgctagatt cgccaacaaa atatccggcc
    18961 tttgccgcaa cctcggcaaa taggcttcca aaaatactgg ttctactgac tgtcccagcc
    19021 aatccatgaa gcattccttt aactgtaagt gctcttttga ttctctttca ctaaagaccg
    19081 aacagttatc tgatttttga tgggcaaaat gagcacattt tttctttccc tttttcaaaa
    19141 tcactctttt tcgacagcca cgacaataat atttctttcc tttttctgca tgtttagcaa
    19201 aacagcactt attatattca ttcattgcaa gcagcatttt tctccctctg aacttattta
    19261 cgtctaagaa tttatttaca cgaaaaaaga gggcgtgaaa aagctattct gcatcaagta
    19321 ataagaacag ctaaacaaaa atagttccca atatttttcg tttggttggg cttgttaccg
    19381 cagatgcaag cttttgaaca catttatacg aaaaaaagac tgcgacacaa tttttgtcac
    19441 agtctcttac tataatgctg atttatacaa aataaaattt cgttcgctcg atagcattac
    19501 gcgccatcag acattgtcca tattcattca atgtttctgg tgtacgatcg ctcttttcag
    19561 caaactccaa aattcgagca atctgattct ctacatccgc ttggttgaga ttttccatcc
    19621 agaagtgaac gactaggtaa tagcgctctt cgaataaata caaatcagtc caagcagact
    19681 ctagttcgac atctgttgca agttggatca cttgttcaaa atcttcaaaa gcaaaagttg
    19741 cttgtctttc ctgttcttct agggcgcttc cagataattc ttcctccgga tcgctgccaa
    19801 taatctgctg acgaagaaag tcactcacat cttctgaatc aacagtagaa tcttctgatt
    19861 gagaaatttc ttcatccgga agatttttac tgataaacag ttctaatccg tctccttttg
    19921 gcaaaacttg gaaagtgaca gcttcgcttc ctcggaactc ctcttcgata tcaacttctt
    19981 ccaaaatact ataaaagaaa ttttcgattt ctttgtgatt gccaagtaaa tccaaaaatg
    20041 aaacgccccg ttcctctaaa tcttcatgac caattagtac acggatcgta ttttcattaa
    20101 tatgttccat ttccattttg ctacacctca ctttgccaca atgtttaata tagattcatt
    20161 gtagcgaaat cagcttaaat gtaaagaaaa aaaatctcgc tgttaagcga atttaataaa
    20221 accagcggat ttttcaacct ttcaagctat cttatttaac tataacattt ttggcagacg
    20281 aatactatat agccgacttt tcagctaatt ttccctctct atttttcgtg caatcatcat
    20341 cttctactac ctttttatac atctaatcaa gaatactttt tgtaaaaaca aaaaaacttg
    20401 gaaaaactat ctttgtaata gtcttttggc tctatacttt ttgatgttgg tgaacttcac
    20461 ca
//
LOCUS       DANKAZ010000043        19864 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000043 DANKAZ010000000
VERSION     DANKAZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            287..841
                     /locus_tag="PWY67_001548"
     CDS             287..841
                     /locus_tag="PWY67_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2057825.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1014..1487)
                     /locus_tag="PWY67_001549"
     CDS             complement(1014..1487)
                     /locus_tag="PWY67_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2057826.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1499..2035
                     /locus_tag="PWY67_001550"
     CDS             1499..2035
                     /locus_tag="PWY67_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2057827.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2075..2446
                     /locus_tag="PWY67_001551"
     CDS             2075..2446
                     /locus_tag="PWY67_001551"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2057828.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2730..4001
                     /locus_tag="PWY67_001552"
     CDS             2730..4001
                     /locus_tag="PWY67_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2057829.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4246..5271
                     /locus_tag="PWY67_001553"
     CDS             4246..5271
                     /locus_tag="PWY67_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2057830.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5565..6674
                     /locus_tag="PWY67_001554"
     CDS             5565..6674
                     /locus_tag="PWY67_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2057831.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6757..7653
                     /locus_tag="PWY67_001555"
                     /pseudo
     CDS             6757..7653
                     /locus_tag="PWY67_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7669..8493
                     /locus_tag="PWY67_001556"
     CDS             7669..8493
                     /locus_tag="PWY67_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2057832.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8525..9835
                     /locus_tag="PWY67_001557"
     CDS             8525..9835
                     /locus_tag="PWY67_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDL2057833.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9983..11242
                     /locus_tag="PWY67_001558"
     CDS             9983..11242
                     /locus_tag="PWY67_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2057834.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11252..12124
                     /locus_tag="PWY67_001559"
     CDS             11252..12124
                     /locus_tag="PWY67_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2057835.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12136..12966
                     /locus_tag="PWY67_001560"
     CDS             12136..12966
                     /locus_tag="PWY67_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2057836.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13006..15189
                     /locus_tag="PWY67_001561"
     CDS             13006..15189
                     /locus_tag="PWY67_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2057837.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15250..16470
                     /locus_tag="PWY67_001562"
     CDS             15250..16470
                     /locus_tag="PWY67_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDL2057838.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16467..17927
                     /gene="gtfA"
                     /locus_tag="PWY67_001563"
     CDS             16467..17927
                     /gene="gtfA"
                     /locus_tag="PWY67_001563"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDL2057839.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18004..19110
                     /locus_tag="PWY67_001564"
                     /pseudo
     CDS             18004..19110
                     /locus_tag="PWY67_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19432..19641
                     /locus_tag="PWY67_001565"
     CDS             19432..19641
                     /locus_tag="PWY67_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2057840.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19634..19783
                     /locus_tag="PWY67_001566"
     CDS             19634..19783
                     /locus_tag="PWY67_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDL2057841.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
BASE COUNT         6790 a         2988 c         3857 g         6229 t
ORIGIN      
        1 aacttaaatt tcatataatt taattatgta aattagctat tctaagaact tctacggata
       61 actgcccgtc taaaatttaa ataagagctt agaatcttca tataagtaag gttaattttt
      121 ttgtacaaaa aagtcggaat attctgagtt actgaaatga tataaaatca taaactaaat
      181 cccttgattt tattgacttt caaaagttta tatatataag ttaatgatta tggtaaaata
      241 tatacataat cattaactca gaatattttt aagaagggaa acaatagtga gctataatgt
      301 acaaccgtta aggacaaatg aagagataca agattttcag ttttggctaa gaagaacaag
      361 taatccggaa agagatagtt ttttattttt gtttggaatc aataatgggt tacgtatgtc
      421 agatattata ggattaaaag ttggagatat acgaggcaaa agtaagccta ttattgttga
      481 acataaaaca ggtaagcgaa aaccgatttt tatagataat ttgagagaag aaattttatt
      541 atatactgaa ggtaaagaag aaaatgattg gttatttccg agtcggcaac aaggtcgaca
      601 tataacaagg gatcgtgtct atcagatata tgcagatata gcagaaaaat taggacgaga
      661 tgatatagga acacatacat taagaaaaac ttttgggtat cactactaca agaaaactag
      721 agacattgct accttaatgt ttatttttaa tcatagctcg caagcaatta caaaaaggta
      781 cattggtata actgaagatg aaataggagc atcacttcgt ggatttaaac taggtgtata
      841 atttaaaaat ttttataaac agtaaagaca aagattaaaa gctattttta tctagaacga
      901 ttttatttca taaagttcgc ggaataaaag ctggggttac tggtacttgg tataggttcg
      961 tgttatataa actaagtaat taaataacat ttttaggttg gaaaaggatg aaattaaaaa
     1021 aagagaatca atacggtctc aaaaacgcaa acgctgcgga ataagaaaaa tactgataac
     1081 tcaacgttta gcagactatc taatagggat agtctgcttt tttcgttttt gaccatactt
     1141 ttgaccttct gcaaacttcc ttgcttttcc tctgtagaac tcatgaaatc tgcaaaacga
     1201 attgccgttt cctctgtctg ctttttcgcg atatgggtgt atatatcatc catagtggtt
     1261 ttaatatctg tatgtccaag tcgaacatac acttctttga cagacgcacc agattcaaag
     1321 agcagactac aatgggtatg acgaaaacca tgaagtgtga tatgttttaa gttattctct
     1381 ttaatgatga ttttcaaatt gtgatttaca tagtctaaat aaaggggctt attttcttct
     1441 gatgaaaaca aaagttgttc accatctttt ataggtttga ttctcatttt tagatattat
     1501 gcccgcacgt attcatcaca tttctattgt gaatcgcttt attcgaccga catttgactt
     1561 ttatcataat atactcggtt taaaattact aatgaaaaca attaatcaag acgatcatac
     1621 catgtatcac ctgttttttt cagataatca ccaccgtgtg ggaactgaac tgactttttt
     1681 tgaagtacaa gaagggaaca accagttttt tggaaccaat acgattgaac gaacgatact
     1741 aaaagttcct tccgaagcat cattgcattt ttgggaaata tattttgaaa cacaaggcgt
     1801 ttgccattat ggaatcgaat cattttctgg acgacctatc ttacgttttg aagggccaga
     1861 tgcaacacga ttagctttag ttcccttacg cgaatttgaa gatattgatg attatttccc
     1921 gtatgtcaca gaccagatcc ctacagagca tgccatttta ggaattgatg ccattcaact
     1981 acgcgtacaa tattccgatg cgactgaacg agagttattg tcggtaggct ggtagcacaa
     2041 agaatacgtc agtttctttt cgggtcatca acgagtgaca atcttagaaa atcaagacac
     2101 tcagttctat caagaagttc agattattca agatcgtgag aatccagttg ccattgaagg
     2161 gattggtagc gtccatcatg ttgcgtttgg cgtagaaaat aaatctgatt tacaacgcat
     2221 cgacaaacaa ttacaagagc gcaattttat aaattcagga ataaaagatc gtgaattttt
     2281 tgtatcactt tattatcgtg acccaaatca attattgatt gaaattgcta caggggaagg
     2341 aaacctagat gcaaaagcgt atgaaaatca aagtccacgc tttgaagaaa ttcctttatt
     2401 tttaccgcag tacttaaatt tcattcgtga acaagtggag caataattag ctaatcaaat
     2461 ccatgacgaa gagtaagagg ttgactcata agtcaaaaaa taaagaaaaa cgcatcaaat
     2521 ccattctaaa gaatgttgat ttgatgcggt tcctattttt ataaactatc attttcgact
     2581 aataaaacta cttatgagtc agcctctctt tttttcttga tttttatacc tctttctagg
     2641 taaactaaac atctgccaaa aataccgtgt ctcattgaaa cattctcaac tatttcaaaa
     2701 taaaggggag taccaaatga acgagtatat tgaaattatc cacgcaagag aaacaactta
     2761 aaaatgtcag tctcaaaatt cccaaaaata aaattagtat ttttaccagc gtatctggtt
     2821 caggaaaatc atcgattgtt tttgatacta ttacccaaga agctggtcgt caactgaatg
     2881 acacctatga tagttttacc cgtttatttt tacctaaata cagtcgtcca gaggttgatg
     2941 agattgataa tctttctacg gcgattgtgg tcaatcaaaa gaagattggt ggcagtgcgc
     3001 gctcaacgtt agggacgatt actaacatca atgcgttatt tcgtgtgttg ttttcatgtt
     3061 ttgcaacacc tagtttagga tatgcaaatg cttattcatt taatgatcct agtggaatgt
     3121 gttctgtctg tcagggaatc ggtaagacga ttgcattttt aataggattg ctttattttt
     3181 cggctattga taatgctgat tttgtatttt ctgatccatt taccgcagaa gttttgtctg
     3241 gaattgagga acaggcaaag aatcatggtt attttgtatt ggtgcataat gttaattcag
     3301 cagaagatgt taaaactatt cagagaaatt ggcgttttga ggggttcatt gttgttggtg
     3361 tacgtgtaaa agaatttttg gaattaaata acgtttttga tggtcctgtt gtttatattg
     3421 atacgcatct ttcaaaagag acattaagag aacttgaaca acaaatgaat cgtttatttg
     3481 taaatacaaa tgattaccaa gctagtacat tggcagtgga acatttgatt cattatggtc
     3541 atgagaaaat agcgtttctt tcttttgact acattgtagg tgaaccaagt gttattcaag
     3601 aacgacatca agcttatatc gatacattaa agaaatatca tttaaccttt aaatcgcatt
     3661 ggatatataa caactctgaa tttgagcgaa tttatcaaga attagctgaa tttactgcaa
     3721 ttgttgttac tggagattat ttagcggcta aattagtaaa atttttaaaa aataagaata
     3781 aacgagtgat ggaacaatta tcgattattg gatttgatga tattactttt gcagaattaa
     3841 tggatcctgc gttgacaaca atacgactaa atccaggtaa caagggggcg attgcttttc
     3901 accaactgat tggactggcc aaacaagaag aaataaaaaa tcaagtacta ctattagagg
     3961 gagaattgat ttctcgagac tctgttcaca aaatcaaata aaaaagctct gtgaaataag
     4021 tatagttaac aataaaatat ccgaacgata gagagaatga actgtgatct agcagttcct
     4081 cgtcgcaaat tcactagaat tagcattatt tactctaaat tgttcggata ttttttcttg
     4141 tttttaatta tgattacagt gcctattttt aacgaaattt gcttattttc aaaatgtatg
     4201 attatatgtt agtaaacact ttcaaatatg aaaagaggag ctattatggt aggaattagg
     4261 gatgttgcaa aacgggcggg tgtgtccatt gcaacagtat cgcgcgtgct aaatgaggat
     4321 ccgacacttt cagtaaccga tgaaaccaaa cgaaagattg cagaatcggt taatttttat
     4381 cattatgtca aaaaaagtcc cgttgcaaaa aatttaacaa atattgcatt aataacgaca
     4441 gtttctgaag tagatgagtt ggaagatccc tattttcgaa cgattcgcag agggattcaa
     4501 gtcgaagcag aacaaagcaa agtcaatttg aagaaaatta ttcgtttgtc agaaacaccg
     4561 ttggagcaag acgatttgct aaaatgtcaa ggtgtgttga ttattgggca ggtgctatct
     4621 tctgtgattg aagaagtatc agcaattaat tcgaatgtcg tagtgattga tgaccctagt
     4681 attgaagctg aagtagatgc agtttatact gatttaacaa aagcaacgaa tcagcattta
     4741 gatcgattgt ataaaaaagg acaccgaaac attgcgttta tcggaggagg acgtgttcgt
     4801 ttagatagcc aaggaaataa acacgtgtca gatgatgatt atcgaaaaat agcctatgag
     4861 acgtggatga aacaaaaagg attagaaaaa tatagtcaaa tttatttaga aggctggacc
     4921 acacttgacg gaatgcaggc agcagattta cttttgaaaa aacatgctaa agataagttg
     4981 ccgactgcta tcatggtagg aaatgatcct attgctgtgg gggtttatcg ttcacttcaa
     5041 aaaaatggtc tacatattcc agaagatatt tcgattgtga gttttgataa tattgaagtt
     5101 gctgaatttt taacacctgc attaaatacg gtgaatgtga atacagaaga aattggaaaa
     5161 ctagcagttc gcatggttaa tgaacgaata aaagaagtta gaaatgtttc gatcaaagtg
     5221 attgtttcta ataatattat tgttagagaa agtgaaaaaa atatcgatta aaaaagtaaa
     5281 catccaaaaa agggtgttta cttttttgtt tactaatatt tttagtaaaa aatctcgaca
     5341 aaacattaag taaacagtta tggtgttgtt aacaaaaaga aaacgcattc aaaacaggag
     5401 gaattaagat gaaacatttc aaaaaaatag ctttaacagg gatagctgtt ttaactggga
     5461 cattactcgc agcatgtggt gggggagata gtaattctaa agacgggcaa gtaactttaa
     5521 gctatgcgag ttgagatact gaccaagctc caggactaag aaaaatgtta gatgaatttg
     5581 aaaaacaaaa tcctgatatc aaagtagaaa tggagacaac gccttgggat caatattggg
     5641 ttaaattaga agctgcaaca acgggaggta atatgccaga tgtagtaacc atgcactcta
     5701 gcgaatccta taaatatatg tcacagaatg ctttgatggg acttgatgat gttatcaaag
     5761 aaaatgatat tgacatggga aactttactg aaggaattgc tgatttctat acgcttgaag
     5821 acgaactata cgcaatccct aaagatgctt ctgtagtggg gctatggtac aacaaagaat
     5881 tatttgataa tgcaggtgtc gattatccag atgaaacatg gacatgggaa acactcagac
     5941 aagctgcaat tgatttaaca gatgaatcaa aaggcatcta tggttttgca tcacatattg
     6001 gaacagagac tggctactgg ccatttattt atcaaaatgg gggagaagta tttgccgatg
     6061 acaatacaaa atcaggtttg aataccccag aagcacaaga agctttagct ttttatcgag
     6121 atattgcact tgttgatcat gcttcaccaa caattacaga attacaagat cctgataagt
     6181 taagtcgttt tcaatcaggt agattagcaa tgatgattga aggaaactgg tttgcaccgg
     6241 gatttttgaa aaatgactat ctagttgaaa atgttgcttc tgccgtgtta cctaagggaa
     6301 aagttaatgc aacaattaca aatggacttg gttgggctgc cagtgcgagt acaaaatacc
     6361 cagaagaagt gaaaaaacta atggcatttt tagctagtga ggacgccaat aaaatccaag
     6421 cggaaacggg ggcatcaatc ccagcactaa aaggctttga agacgagtgg gctgcttctt
     6481 cagatacaat tgatttgagt gcttttggaa aaatgttgga gtatggagtc cctcgtccat
     6541 acaataaaga tggattacga gctgaacaag cagaagttca aattattaac cgtattctgt
     6601 ctggtgaagt aactgttgaa gatggaaccg ctcaaattga aaaagaagtc aatgcgatat
     6661 tgagcggaaa ttaaattagt taattataca atatacaggc gaaaggattc tatttcgtct
     6721 gtatattaat ggatgaatac caaaaagggg gatattatga aaaatcgaat ttttggaaaa
     6781 tatcgtaaag atcaagtgat atggggctat atttttattt ttccaacggc attaggatta
     6841 attttattaa atatatggcc agcaattcaa acagcaattt taagttttca aaaaacggta
     6901 gggttcggtg atactgaatg ggtcggatta agaaattacc aaaaaatgtt tgcagacggt
     6961 gaagtttttc agtctttatt aaacacatta atttatgcag tggtttcgat acctagtatt
     7021 gttgtattat ccttgttggt ggcagtttta atgaatagaa aagttaaagg attgtcgatt
     7081 tatcggacaa tttattttct accagtcgtt gctgcaccat ccgcagtcgc aatgatttgg
     7141 cgctggatgt tcaataatga ttatggactg attaataaca tgttggctaa aataggtctc
     7201 gatggaccag catggttaac ggatccatcg attgcgattt attctattat cattgtagga
     7261 gtttggagtg ctattggtta taacatggtg ttattactag ccggcttata agaaattcct
     7321 aaagattatt atgaagcagc ggaaattgat ggtgctagtc caatccagca gtttttcaac
     7381 attacgttac cattagttac accaacatta ttttttgtag ttgtaacaac agttatcaat
     7441 gctttccaag tatttgatgt tatttttatg atgattactc ctaatagtac cgccatgttt
     7501 aaatcacaat cattagccta tttattctat aaacattcat ttattttaaa tgacaaggga
     7561 tatggttcgg caatcgttat gttcctttta gtgattatat taattattac tgcaattcaa
     7621 atgaagttgc agaaaaagtg ggtaaactat taaggaggct atcaaattat gaaaaataaa
     7681 tttttttcaa atgcgccaat ccatatcatg cttattttag gtggtattgc aatggcatta
     7741 ccttttattt ggatgatttt aacttcaggg aaaacgttgg ttgaatctac ccaaattcca
     7801 cctacaattt tccctgagaa atttcagcta agtaactatc aacaagtact tgatttatta
     7861 ccattcatgg ctttttatat caatacattt ttaatggttg ttggacgagt gattggctcg
     7921 gttttatttt cagcaatggc tgcatatgct tgtgcgcgtc taagttttcc agggaaaaat
     7981 atccttttca gcttagtact tattcaaatg atgattcccg gttccatttt tattactcca
     8041 cagtatttat tagcacaaaa attgggtatt ttaaatacaa ttccagcatt aatattacca
     8101 ggggtagtta gtgcgtttgg tacgttttta ttaagacaat ttttcttagg tttacctgat
     8161 gaattggagg aagcggccta tttagaaggt gccaatattt ggacgatctt tttcaaaatt
     8221 atgttgccgc ttgctaaatc aggattggtt gcacttggaa tattcacagc gttgtttgca
     8281 tttaaagatt taatgtggcc attgattgtc aatacttcaa ttgataagat gcctttagct
     8341 ccgggattag caaacttaca aggacaatat gccacgaact tcccacaatt aatggcaggt
     8401 tcgttattag caatttggcc aatagttgtg atattcatcg ttttccaaag aaaattcatt
     8461 gaaggaattg ctacaacagg tagtaaatta taaaactatt aattagtgat tggagagtaa
     8521 gtacatgaca aaaattacat ttattggtgc aggaagtact atttttgcaa aaaacgtatt
     8581 aggcgatgcg atgttaactc caagtctaca agatgcacat attgcattgt acgatattga
     8641 tgaaaaacga ctaaaagaat caaatttaat gcttaaaaat attaataaaa attcgaatga
     8701 aaatcgtgct gatattgttt cttattatga tcgcaaagaa gcgttaagag aggctgattt
     8761 tgttatcaat gctattcaag tgggtggtta taaaccttca acagtgattg attttgatat
     8821 tcctaaaaaa tatgggttgc gacaaactat tggtgacacc atggggattg gtgggatttt
     8881 tagaacccta cggacactac cagtaatgtt agactttgct aaagaaatgg aagaagtttg
     8941 tccaaatgct tggttattga actatacaaa tccgatggcg atgttaacca tgggtgtttt
     9001 aaaagcgacg agtattaaga ctgttggttt atgtcatagc gttcaagtat gcgtaccaga
     9061 attgtttgag catttaggaa tagccgatca atatgactta aatgactttc aatggaaaat
     9121 tgcaggcatt aatcatatgg cattcttgtt ggaaattaca aaaaatgggg aagatttcta
     9181 tccaacaatt cgtcagttag ctgcagaaaa accaaatcca catccagatt ctgtacgttt
     9241 tgaattgttg aaacgttttg gttactatat tacagaatca agtgaacaca atgcagaata
     9301 ccatccatac tttattaaat caaaataccc agaattgatt gacgaattag ggattccgtt
     9361 ggatgaatac ttacgtcgtt gtgaacaaca aattaatgat tgggaaagca tgcgagagga
     9421 cattgtaaat aatggggagt taacacatac gagaagccgt gaatatgctt catatatcat
     9481 ggattctatt acgacaggaa ctccgtcagt gattgcagga aatgtcttaa ataaaggctt
     9541 aatcacaaac ttaccgacag atgtttgtgt ggaagtacct tgtttagtcg ataaaaatgg
     9601 cgtgcaacca acctatgtcg gtgaattgcc aacacaatta gctgctttaa atcggacgaa
     9661 tatcaatgtt caagaactga cagtagaagc cgctttaaca ctagataaat caaaaattta
     9721 tcaagcagcg tatttagatc cacatctatc atctgaattg tctttaacag atatcaaaaa
     9781 tttagtggat gatttaatcg aagcacatgg ggactattta cctgaatata attaaaaaga
     9841 agctgaaaga gtaaggaatt ttcaattcct tactcttttc ttagttaaat ttagtaaaaa
     9901 tattgacaaa acacttagta aacagttaca ttagtgttaa caaaaagaaa acgcattcaa
     9961 attgtataag gaggagcata aaatgaagtg gtctaaaaaa attattttag cttgtatagc
    10021 actaggagca acgatctctt tgactgcttg tggaaaaaat caggaagatg caagcggtaa
    10081 ggtagaaata gagtttttta atcaaaaaaa agaaatgact caaacgattc aggagattgc
    10141 taaagatttt gaagcaaaaa accctgatat tcatgtaaaa gttgtggatg taccgaatgc
    10201 tggtgaagta attaaaacac gtatgcttgc aggcgatgta ccagatgtca ttaatcttta
    10261 tcctcaaagt atagaattga aagaatgggc gaaagctggt tatttagagg acttaaccga
    10321 agaaccgtat ttagagaata tcaaaaatgg atatgcacaa cgttttgcta ttgaggatcg
    10381 tgtatatagc ataccattaa ccgctaatgt ctatggtttt tactataaca aaactgcttt
    10441 tgaggaaatg gggattcaag caccagaaac ttgggcagaa tttgaaaaaa tagtagctga
    10501 gattaaagat caaaacaaag taccgtttgc gattgcagga gcagaaggct ggactttaaa
    10561 tggttatcac caactagcta tagcgactgc tgctggtggt gaagaggaag cgaacaatgt
    10621 ttggcgtttc tcagaagtta atggaattaa tgctgaaagc aaagaaatgc aaaaagattt
    10681 taatcgatta gatttgttaa gagaaccaaa agctttgcaa aacaactggc aaggtgccgg
    10741 ttataacgat acagtagtta cttttacaaa aggtgatgcg ttgattatgc cgaatggttc
    10801 atgggcaatg ccgatgattc attcacaaaa tccagatttt gaagtaggaa cttttccttt
    10861 tccagcagat gaagcaggtc aaagtctaac gattggcgca ggagacctag ccttatcagt
    10921 ttctgcaact tctcaacata aagaagaagc caaacgtttt gtagaatata tgacaacacc
    10981 tgaagcgatg cagaaatatt atgatgtaga tggtgcaccg tgtgctgttg agggcgttat
    11041 cgagaatact caggattcac cattaagtgg tttaactgaa cttgctttta ctgatcgtca
    11101 tttagtttgg ttagccaaag attggaatag tgaaaatgat ttttatacct taacaacaaa
    11161 ttatttacac aatggcaatc aaaaagccat gattaacgca ttaaatgctt tctttaatcc
    11221 aatgaaagct gacggaaggt aggaaaataa aatgaaaata aaagaaattt taaatcgtta
    11281 ctgggcaatc atttttgtag caattccaat tattttgcag atgatttttt tctactttcc
    11341 aatggttcaa ggtgcttttt acagtttaac taattggact ggtctgactt ataattttga
    11401 ttttgtgggt ttgaacaatt ataagattct tttttctgat ccgaagttta tcaaatcgat
    11461 tggcttcact ttaattgtga cgctttgcat gattgctgga caaattggtt ttggtatttt
    11521 gattgcgcgg gcgttgaacg caaatattaa agggaaaaca ttctttagag catggttctt
    11581 ttttccagcg gtcttatcgg gcgtggttgt ttctttagta tttaaacaaa tttttaacta
    11641 tggtttacca aaaattggta gtatgttagg cattgaattt ttacaaacca gtcttttagg
    11701 aacaacatgg ggagcgattt ttgcagtaat ttttgtttta ctttggcaag gaattgccat
    11761 gccagtcatc attttcttag cgggttttca aagtattcca caagaaatta ttgaagctgc
    11821 tagaatggat ggggcgaatg ataagcaaat tttttggaat attgaaatca cctacttatt
    11881 accaagtatt tcaatggtat ttattatggc attaaaaggt ggcttaacag catttgatca
    11941 aatttttgct ttaactggtg gtggaccaag cgatgccaca acatctttgg ggttacttgt
    12001 ttataattat gcttttaaaa acaatcagtt tggttatgcg aatgctattg ctttagtctt
    12061 atttatctta attgggattg tttcactggc acaattaaaa ttatcgaaaa aatttgaaat
    12121 ctaggaggtt tcccaatgaa aacatcaaaa gaaaaaaata tttctaaata cttgctttta
    12181 ttattagggg gcttcatgat tttagttccc ttagtttcaa cgctattcgg ttcatttaaa
    12241 acaacacccg atattatgaa taatttcttt cgttttccta atccagcgac tttggaaaat
    12301 ttccgaagat tgttagcgga tggaattggt cattactttt ggaattcggc aatcatcaca
    12361 atcattgcag tagttgctgt gacagcaatt attcctatgg ctgcttattc actagcaaga
    12421 aaaatgactc gtgggaaagc atacggattg atgtatttat ttttaatttt aggaattttt
    12481 gttcctttcc aagtaattat gattccgatt accgttatga tgagtaaaat tggtttagca
    12541 aatatgtggg gattaatctt actctatctc acttatgccg taccacagac actattttta
    12601 tatgttggtt ttattaaact aaatgtgcct atcagtctgg atgaggctgc tgaaattgat
    12661 ggggctagtc actttacaac ttatttcaaa attgttttcc caatgttaaa accaatgcat
    12721 gcgacgacta ttattcttaa tgctctatgg ttttggaatg atttcatgct accattatta
    12781 attttaaatc gtgattctaa tatgtggaca ctaccattgt ttcaatataa ttatacggga
    12841 caatatttta acgactatgg tccaagtttt gcttcctata cagtaggaat tgtcacgatt
    12901 acactagttt atcttgcttt ccaaagaagt attatagctg gaatgagcaa tggagcagtg
    12961 aaataaccaa taaattattc tcattataaa ggagttcata aacttatgat tatatttgac
    13021 aatcacacac aaacatttca tttacaaaat aaaaaaatta gctatctatt aggaatcgaa
    13081 gaaaataatt atttaacgca tctttatttt ggaaaaaaga ttacgcacta ttctggtggg
    13141 tatcgctacc cccgaacaga tcgttccttc tcacccaaca atgcaaatgc gcaagatcgt
    13201 ttattatctt tagacacatt accattagaa tttccaggat atggacatgg tgattttaga
    13261 gaaccggcgc ataatattaa attaggaaac ggtagtcgtg taaatgattt tcgttatgat
    13321 agctacgaaa ttcaaaaagg aaagcccagc ttagcgggat tgccagcctt atatacggaa
    13381 gatgatgatg aagctgaaac gctgattatt caattgaaag acgcgattag tggattgact
    13441 ttacagttgt tctacacaat ttatcaaaat cacagtgtaa ttattcgttc tacgcaatta
    13501 gtaaataata gtgatgaaac agtggagatt aaccgcttag ctagtcaatc aattgatttt
    13561 ccaaatcgtc cactcgagtt aattcattta aatggtacct gggcaagaga acgtcaaatg
    13621 actagagaaa aaattcatac agggataaaa gtattagata gtaaacgcgg atcaagcagc
    13681 catcaacaaa atccgtttgt tgctttagta gatgccaaaa cagatgaatt tcaaggagag
    13741 gcatatggat ttaatttagt ttatagcggt aatcacgaaa cggtgattca aaaagatcca
    13801 tttgaccaaa cacgggtcat tatgggaatt aattcattta attttggttg gcaactaaaa
    13861 actggcgact catttcaaac gccagaagtc gtgatggttt atagtaatga aggtctgaat
    13921 acgttatcac agacgtatca tgaattgtat aatcaccgtt taattcgtgg aaactatcgt
    13981 ttaaaagaac gtcccacgtt aatcaacaac tgggaagcaa cgtattttga ttttaacgaa
    14041 gaaaaaatca tgaatattgt cgatgatgcg aagaacttgg gcattgaaat gtttgtttta
    14101 gatgatggtt ggtttggcgg tcgtgaaaat gacttcactt cattaggtga ttgggttgaa
    14161 acagaaggca aacttgcttg tggtttggaa aatttagcca agcaagtgca tgataacggg
    14221 atgaaatttg ggatttggtt tgaaccggaa atgatttctg aagatagtga actatttcga
    14281 gcacatccag attgggcatt gggtattcca ggtcgtgacc gctcaattag tcgaaatcaa
    14341 tatgttcttg atttttcaag acaagatgtt cgtgataata tctatcaaca aatgacggcg
    14401 atttttgatc gtgtaccgat tgattatgtg aagtgggata tgaatcgaaa tatgacagaa
    14461 gtctattctg tcttgttgga ctcaacgatg caaggtgaag tggctcatcg ttatatgtta
    14521 gggttatatg aatttttaga aaagttaaca acgaattatc caactatttt atttgaaagt
    14581 tgttcgggtg gaggcggtcg ttttgatgca ggtttcttgt attacatgcc acaaacttgg
    14641 acgagcgaca ataccgatgc cgtggctcgt ctgaaaattc aatatggaac cagcttagtg
    14701 tatccaattt caagtatggg tgcgcatgtt tcagcagtcc caaatcatca aacaggaaga
    14761 gaaacgagtt taactattcg tggcaatgct gcgatggctg gtgtttttgg ttatgagcta
    14821 gatctaacgc aattaacttc tgaagaaaaa gcagaaatta aagaacaagt tgccttttat
    14881 aaaaagcatc gccaactttt gcaatatgga actttttatc gcttggaaag tccgtttgaa
    14941 ggcaatgata ctgcatggat gtttgtctca aaagaccaaa cagaagccat ggtcttttat
    15001 ttccgagtat tagcagaagc tgcgcatcca ttagtcacat tgaaattagc aggattagca
    15061 gaagaagcaa cttatcaaat ggaagatgta acatttgctg gcgatgaatt gatgaatctc
    15121 ggtttttata ttgactcaga actacatggt gattatgcga cacaacgctt ttatttgaaa
    15181 cagatatagt agcttgaatt tacaatataa gtgcaacacc aaaagagact cattccaaac
    15241 gaagaaaata tgttttattt acacgcattt actaaaaagc agcctgattt gaattgggaa
    15301 aatgaagaag tacgtgaaga aatctatgca atgattaact attggttaga taaaggacta
    15361 ggcggattta gaatcgatgc gatattaaac atcaaaaaaa aactagagta tggcatcttt
    15421 gaatctgatg gagaagatgg gttagccttc attgggccct ggattcttaa tcagtcgggc
    15481 attgaagtgt ggttaaaaga aatgagagag cggacattta aaccacataa tagtatgacg
    15541 gtagcagaag ccgatgttcc taatgaacgt ttagatgaat atattggtga agatggctat
    15601 tattcgatgg tttttgattt tagttatacg gatattgatg tacctgaaac tggtgaatgg
    15661 tttaaagata gccaatggac atggacggat atgcgtacca atatttttac gaatcaatta
    15721 gttacgcaag ataaaggttg gggcgcctta tatttagaaa accacgatca accacgttca
    15781 attaataaat atattccaga agaatggatc aatgattata gtaaaaaaat gttggcgacg
    15841 ctttttatgt tactaagagg tacccctttt atttatcaag gacaagaact aggaatgacc
    15901 aacatagaaa tggcatcatt agaagatttt gatgatgtag ctacgcattc tcagtataaa
    15961 cgggcattag aatatggatt ggcaccagag caagcattga aagctgtcaa taaacgaagt
    16021 cgggataatt ctcgcacacc tatgcaatgg aatacgcaaa aaaatgccag attctcaact
    16081 tctgataatg tatggttaaa agtaaatcct aattatccag aactcaatgc agaagctcag
    16141 ttaaccaatc aacagtcggt attcaattat tatcatcaat taattaaatt aagaaaaaat
    16201 agtgtataca aagacgtgct aatctacgga caatttctac cagtgaaaga taaaaatgaa
    16261 catattttgc tttatgaacg ccaattagaa gataaaatcg tattagttat cttaaacgtt
    16321 acagcaacag aacaagaata ttatgtagat gaacagtacc aacaagtatt aatcaataat
    16381 tatgaagatg ttttattaga tcaaagtcga aaattgcgtt tacgaccgtt tgaaagtatt
    16441 gtattagcta attatggagg acaattatga aaattaaaaa cgaagcaatg ctcatcacct
    16501 atgcagatag tttaggaaaa aacatgggcg aattaaaaca agtcttagac aaacatttac
    16561 aaggtgttat tggtggtgta catctgttgc ctttttttcc atcgacaggt gatcgaggct
    16621 ttgcgccgag tgattatacg acagttgatc cagctcttgg aacatgggaa gatgtggaag
    16681 cattaggtga agattattat ttgatgtttg attttatgat taatcacatt tctcgtgaat
    16741 caaaattttt ccaagatttc aaaaaaaacc atgagcagtc accatacaaa gaaatgttta
    16801 tccgtattca tgaatttttc ccagaaaatc gaccaacaca agaagatatt gatttaatct
    16861 acaagcgtaa agacaaagcg ccgtttcaaa cggtagaatt tgcggatggt acgacagaag
    16921 aggtttggaa tacctttggg gaagaacaaa ttgatttaga tgtgaccaaa gaagttgtga
    16981 aagaatttat tcgagaaacc atcaaagata tggcgaacca tggctgttct ttaattcgat
    17041 tagatgcgtt tgcttatgcg attaaaaaat tagataccaa tgatttcttc gtggaaccag
    17101 atatttggga acttttagac gaagtgcgtc aagaagcagc gaaatatgac gtcgaattat
    17161 taccagaaat ccatgagcat tattccattc aaatgaaaat tgccaatcat gattattata
    17221 tctatgactt cgccttgcca atggtcactc tgtattcact ttatagtgga aaatcagaac
    17281 gtttagccaa ttggttgaaa atgagtccga tgaagcagtt tacaacattg gatacgcatg
    17341 atggaattgg tgttgtggac gctcgtgatt tattaacaga tgaagaattg gattacacct
    17401 ctgaggaatt gtataaagtc ggggcgaatg tgaagaaggt ctattcaagt gccaattaca
    17461 ataatttaga tatttatcaa attaacagca cctattatag tgcattaggc gataatgacc
    17521 gtagctatct gttagcacgt gcgattcaag tatttgcgcc agggattcca caaatttatt
    17581 acgttgggtt attagcagga aaaaatgata ttgaactgtt ggaagcaacg aaagaagggc
    17641 gtaatattaa tcgtcattac tatgatttag ccgaaattga agaagaagtg caacggccag
    17701 tgattcaaca attatttgca cttttaaaat ttagaaatga atctgcggcg tttgatttgg
    17761 atggaacgat tgaagtagca acgccatcag aatcagaaat agtgattaca cgtaaaaata
    17821 aaaacggtag tgatagtgca acattaacgg ctaacttgga tacgaaagaa tttatcattg
    17881 agtctaaaga tataaaaatt ttcactagtg ttgcattaaa attttaacct attccataga
    17941 acaaaaaatc ctttatacta gcacttggat gacttatcca agaccaataa aaaggactca
    18001 tacatggata agtataaaac aaatacagca tttaataaat ggttttctgc cataaaatta
    18061 ggaaaacttt ctgtcaactc ccaaaaatgt atttttgatt acaattcgta tcgtaaaaaa
    18121 ctgtcttttg aagctgtgct caagctcttt ctctacgcag ttaatgaaga aaaagaaagt
    18181 ttgcgtgatt tgagtacagc tcttatcgac cgatcgctcc aactagaaac cgatgtgtca
    18241 tcgattagtc atacccaatt gtctcgagca tttaatgcac ttgaacctaa agtgttagaa
    18301 gaaatttttc aacaactgtt agaaaaagtt caacatcaaa tcaaaccaac caagcgaaat
    18361 catctgtatc tgattgattc cagtacgttt tcgcttagtt taaaacgtca taagtgggcg
    18421 actttccgtc aaacaaaatc aggtgtcaag ttacacttaa actattgtta tatggacgat
    18481 actacgatgt atccaactga ttttacctta acaaatgccc atgaacatga tctcaatcaa
    18541 ctaccagtat tggtgaatca accagaggca acttacgtgt ttgatcgtgg gtattaggac
    18601 tttgaaaaga tggatcagat gcattgggat ggctatttct tcgtcactcg gattaaaaaa
    18661 aacaccaaag ttcacgtgat agatactctg gaaacatcgc cagaaacaga aattctacga
    18721 gacgagctgg ttcgtttagg atctaaaacc tatctaaccg ccaatttccg actagtcact
    18781 gttcaagata agaacggacg tgtgtttcaa tttatcacca atcgaatgga cgtctcttca
    18841 aaagaaatct ctgatatgta tcacgctcgt tggcaaatcg aattgttttt caagcacatc
    18901 aaacaacaca tgaccattaa aaccttcttt tctcaaagtg aaaaaggcgt gcagaatcaa
    18961 ttgattttaa cgatgatttc cgctctcctc acgtttctca tcaaattgga gacgaaaacg
    19021 gaaaaatcag tgttccaaat caaacgattc tttcgttatt tactgtttca gccctttgaa
    19081 tgttgtctag aaaaattaat tcccacatga gaaaaaagaa atttggctcg ttacttttgg
    19141 ttttcagttg aataacagat aaaaaatttt ctggaaaaag tcatcctctg cgtcagcaca
    19201 gttttttttg acttattttc agaggaaata gttttcagaa tattctaaaa tgttttatgc
    19261 aacactagtg atatttttta gaattagatt tctaatgtgc attttttgag tattaaggag
    19321 caagtataca aagatttata aagctgatta aatttgaata aaaatatgca ggaacgctac
    19381 tatttttagt agtgttcttt tttatatttg aataaaaaat gtggtggtta aatggtggtt
    19441 ataacaaaaa atgaattaat gaaattaggt tatgggaagt atcagtctga gagaatcata
    19501 cgacaggcaa aagcaattat ggtttcaaaa agctatacct attacgaaaa tagaagatta
    19561 ggactagtac ctgttgaatc tgtagaagaa gttttaggta tgaagataga ggtagaggag
    19621 gattttcaga atcatggtta attttaaaaa agataaaaat gggaaatatc attttactgc
    19681 tagtttggga ttcgacaaac gaacagggaa aagagtacaa aaaaacgtag aggggttaaa
    19741 acagttaaag aagaaaaaaa gcgtataccg aacttgtttc taattatgga aaagaagcag
    19801 aaacttctca aagtactatg ttgtttgagg agttttttta taaattattc ctaccttatt
    19861 atca
//
LOCUS       DANKAZ010000044        19475 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000044 DANKAZ010000000
VERSION     DANKAZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(21..995)
                     /locus_tag="PWY67_001567"
     CDS             complement(21..995)
                     /locus_tag="PWY67_001567"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2057842.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="PWY67_001568"
     CDS             complement(1075..1950)
                     /locus_tag="PWY67_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL2057843.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="PWY67_001569"
     CDS             2080..3987
                     /locus_tag="PWY67_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL2057844.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="PWY67_001570"
     CDS             complement(4528..7806)
                     /locus_tag="PWY67_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL2057845.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8077..8622)
                     /locus_tag="PWY67_001571"
     CDS             complement(8077..8622)
                     /locus_tag="PWY67_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2057846.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8634..9326)
                     /locus_tag="PWY67_001572"
     CDS             complement(8634..9326)
                     /locus_tag="PWY67_001572"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL2057847.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9345..9629)
                     /locus_tag="PWY67_001573"
     CDS             complement(9345..9629)
                     /locus_tag="PWY67_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057848.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9642..10205)
                     /locus_tag="PWY67_001574"
     CDS             complement(9642..10205)
                     /locus_tag="PWY67_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2057849.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10433..11095
                     /locus_tag="PWY67_001575"
     CDS             10433..11095
                     /locus_tag="PWY67_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL2057850.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11219..12415
                     /locus_tag="PWY67_001576"
     CDS             11219..12415
                     /locus_tag="PWY67_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL2057851.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12516..13892)
                     /locus_tag="PWY67_001577"
     CDS             complement(12516..13892)
                     /locus_tag="PWY67_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2057852.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13929..14795)
                     /locus_tag="PWY67_001578"
     CDS             complement(13929..14795)
                     /locus_tag="PWY67_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDL2057853.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14884..15909)
                     /locus_tag="PWY67_001579"
     CDS             complement(14884..15909)
                     /locus_tag="PWY67_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2057854.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15927..16601)
                     /locus_tag="PWY67_001580"
     CDS             complement(15927..16601)
                     /locus_tag="PWY67_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2057855.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16785..17678)
                     /locus_tag="PWY67_001581"
     CDS             complement(16785..17678)
                     /locus_tag="PWY67_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2057856.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17752..19449)
                     /locus_tag="PWY67_001582"
     CDS             complement(17752..19449)
                     /locus_tag="PWY67_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2057857.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3445 g         6157 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
     8041 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
     8101 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc
     8161 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
     8221 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
     8281 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
     8341 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
     8401 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
     8461 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
     8521 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
     8581 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
     8641 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
     8701 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
     8761 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
     8821 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
     8881 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     8941 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     9001 ggtttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     9061 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     9121 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     9181 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     9241 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     9301 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     9361 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     9421 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     9481 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     9541 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     9601 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     9661 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     9721 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     9781 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     9841 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     9901 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     9961 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
    10021 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccaccagg atgaaatacc
    10081 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
    10141 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
    10201 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
    10261 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    10321 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    10381 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    10441 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
    10501 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
    10561 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
    10621 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
    10681 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
    10741 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
    10801 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
    10861 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
    10921 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
    10981 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
    11041 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
    11101 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
    11161 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
    11221 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
    11281 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
    11341 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
    11401 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
    11461 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
    11521 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
    11581 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
    11641 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
    11701 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
    11761 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
    11821 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
    11881 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
    11941 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
    12001 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
    12061 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
    12121 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
    12181 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
    12241 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
    12301 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
    12361 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
    12421 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
    12481 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
    12541 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
    12601 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc
    12661 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
    12721 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
    12781 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
    12841 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
    12901 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
    12961 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
    13021 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
    13081 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
    13141 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
    13201 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
    13261 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
    13321 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
    13381 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
    13441 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
    13501 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
    13561 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
    13621 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
    13681 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
    13741 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
    13801 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
    13861 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
    13921 aacagcgatt aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg
    13981 caaccaacaa taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc
    14041 ccacctaaag tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca
    14101 agtgatttga ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc
    14161 atgtttttcc acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct
    14221 aagatcattc cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg
    14281 acgatgatgg tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta
    14341 ccaaaatcaa gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc
    14401 aaatacgcac cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt
    14461 tgtgtccatt catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca
    14521 gtagacaatg gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt
    14581 tgtccaacac tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt
    14641 acaggactaa caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct
    14701 agtgtttgtt gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat
    14761 gccaacatag ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg
    14821 ataagaaact tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat
    14881 tttctaacgt ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc
    14941 ttttccttca gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc
    15001 taaataaagt cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa
    15061 tcccttatca aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac
    15121 tgaaaggctg actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg
    15181 cgtatatttg atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc
    15241 ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata
    15301 atttgcctga ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag
    15361 gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc
    15421 gatttttgat aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat
    15481 cgaacgaact agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc
    15541 aatataatct ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc
    15601 acggatcagc tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact
    15661 caaataattt tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct
    15721 cttgctcaag taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa
    15781 ataagcaatt gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt
    15841 caatgcaata aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa
    15901 aaaattcatc ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt
    15961 tcgatatatc cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact
    16021 gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc
    16081 cgacttaaga tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg
    16141 ctcaaatcaa tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg
    16201 ccattgtggc tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat
    16261 gcattgattt ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc
    16321 cccatgttca tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc
    16381 ggtactttcg atacttcacg tattttttga caccaataat agccatccaa aacgggaaga
    16441 ttaatatcta gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg
    16501 aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt
    16561 cgaatgatct cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt
    16621 cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat
    16681 gtcattttag caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt
    16741 ccaagcagct taaatttatt atgttattct taataattgg tctattatct gttatttttc
    16801 tttcgttgtt tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact
    16861 agcaatatac ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct
    16921 atctttgaag agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga
    16981 gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt
    17041 gctaatgtat ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc
    17101 atccgaccat acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta
    17161 aagagttttt tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct
    17221 ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc
    17281 gtttcatcaa ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata
    17341 tgttgttcgt aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga
    17401 tctttgactt gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt
    17461 tcatgcactg ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga
    17521 taattctcga tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat
    17581 gtaaaaactg cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg
    17641 atttgggttt gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa
    17701 aaaactgcag caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc
    17761 agacttcatt ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat
    17821 tagtaagaat gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt
    17881 cggtactctt tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct
    17941 tccataagag atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc
    18001 tggcgccatg gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta
    18061 tccatttcca tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc
    18121 tgctcctgat tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga
    18181 acggtcttca ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc
    18241 atatttttca aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc
    18301 ataatcaaat tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc
    18361 cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc
    18421 atctgctaat ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt
    18481 cacgacaaac ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt
    18541 ctcgccggct cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc
    18601 tgtgattttg taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata
    18661 aggaataggt gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga
    18721 aatcgtttct tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc
    18781 tacattcatc tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt
    18841 attgtatgtg tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat
    18901 cactggcatc ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt
    18961 ttctaagaaa agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt
    19021 agagaatacc gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata
    19081 ttttaaaaac caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt
    19141 aagaccgtct tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc
    19201 cgttacgtca aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga
    19261 aaccaatccg ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt
    19321 aatcgtcact tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc
    19381 gaaaatccct attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt
    19441 tttattcatt tttttctccc cgttcttttt ttatt
//
LOCUS       DANKAZ010000045        19343 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000045 DANKAZ010000000
VERSION     DANKAZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(407..766)
                     /locus_tag="PWY67_001583"
     CDS             complement(407..766)
                     /locus_tag="PWY67_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL2057858.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="PWY67_001584"
     CDS             complement(796..1137)
                     /locus_tag="PWY67_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057859.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="PWY67_001585"
     CDS             complement(1134..1808)
                     /locus_tag="PWY67_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2057860.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="PWY67_001586"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="PWY67_001586"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL2057861.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="PWY67_001587"
     CDS             complement(4322..5512)
                     /locus_tag="PWY67_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2057862.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="PWY67_001588"
     CDS             complement(5533..6060)
                     /locus_tag="PWY67_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL2057863.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="PWY67_001589"
     CDS             complement(6107..8896)
                     /locus_tag="PWY67_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2057864.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="PWY67_001590"
     CDS             complement(9043..9243)
                     /locus_tag="PWY67_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2057865.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="PWY67_001591"
     CDS             complement(9696..10016)
                     /locus_tag="PWY67_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057866.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="PWY67_001592"
     CDS             complement(10519..11637)
                     /locus_tag="PWY67_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057867.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="PWY67_001593"
     CDS             12108..13415
                     /locus_tag="PWY67_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2057868.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="PWY67_001594"
     CDS             13536..14735
                     /locus_tag="PWY67_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2057869.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="PWY67_001595"
     CDS             complement(14762..16030)
                     /locus_tag="PWY67_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL2057870.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="PWY67_001596"
     CDS             complement(16200..16841)
                     /locus_tag="PWY67_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDL2057871.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="PWY67_001597"
     CDS             complement(17250..17753)
                     /locus_tag="PWY67_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2057872.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="PWY67_001598"
     CDS             complement(17876..18640)
                     /locus_tag="PWY67_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL2057873.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="PWY67_001599"
     CDS             18748..19023
                     /locus_tag="PWY67_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL2057874.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         5539 a         4123 c         3444 g         6237 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gct
//
LOCUS       DANKAZ010000046        19248 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000046 DANKAZ010000000
VERSION     DANKAZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            214..1593
                     /locus_tag="PWY67_001600"
     CDS             214..1593
                     /locus_tag="PWY67_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057875.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHP
                     DMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKL
                     VQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQ
                     PRREVQHSH"
     gene            complement(1738..2478)
                     /gene="truA"
                     /locus_tag="PWY67_001601"
     CDS             complement(1738..2478)
                     /gene="truA"
                     /locus_tag="PWY67_001601"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2057876.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2669..3304)
                     /locus_tag="PWY67_001602"
     CDS             complement(2669..3304)
                     /locus_tag="PWY67_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2057877.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(3451..4692)
                     /gene="sstT"
                     /locus_tag="PWY67_001603"
     CDS             complement(3451..4692)
                     /gene="sstT"
                     /locus_tag="PWY67_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL2057878.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4890..5888
                     /locus_tag="PWY67_001604"
     CDS             4890..5888
                     /locus_tag="PWY67_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057879.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5901..7919
                     /locus_tag="PWY67_001605"
     CDS             5901..7919
                     /locus_tag="PWY67_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL2057880.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7979..8695)
                     /locus_tag="PWY67_001606"
     CDS             complement(7979..8695)
                     /locus_tag="PWY67_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2057881.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8922..9464)
                     /locus_tag="PWY67_001607"
     CDS             complement(8922..9464)
                     /locus_tag="PWY67_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2057882.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9469..9696)
                     /locus_tag="PWY67_001608"
     CDS             complement(9469..9696)
                     /locus_tag="PWY67_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2057883.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9775..11655)
                     /locus_tag="PWY67_001609"
     CDS             complement(9775..11655)
                     /locus_tag="PWY67_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2057884.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11844..12458)
                     /locus_tag="PWY67_001610"
     CDS             complement(11844..12458)
                     /locus_tag="PWY67_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2057885.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12600..13160
                     /locus_tag="PWY67_001611"
     CDS             12600..13160
                     /locus_tag="PWY67_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2057886.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13465..13779)
                     /locus_tag="PWY67_001612"
                     /pseudo
     CDS             complement(13465..13779)
                     /locus_tag="PWY67_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14033..15668)
                     /locus_tag="PWY67_001613"
                     /pseudo
     CDS             complement(14033..15668)
                     /locus_tag="PWY67_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16223..16786)
                     /locus_tag="PWY67_001614"
     CDS             complement(16223..16786)
                     /locus_tag="PWY67_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2057887.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(16801..17844)
                     /locus_tag="PWY67_001615"
     CDS             complement(16801..17844)
                     /locus_tag="PWY67_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057888.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(17977..18381)
                     /locus_tag="PWY67_001616"
     CDS             complement(17977..18381)
                     /locus_tag="PWY67_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2057889.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(18614..19246)
                     /locus_tag="PWY67_001617"
     CDS             complement(18614..19246)
                     /locus_tag="PWY67_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2057890.1"
                     /translation="MFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFGD
                     VDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFTG
                     LFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTVI
                     SLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
BASE COUNT         6047 a         3747 c         3062 g         6392 t
ORIGIN      
        1 tttctaagtt ttgcttacgt catccctcta ttcatgtcta agggaatcta tcatagtgtc
       61 aatcaggata cttactttca tttatctcga atcataggac tagataatgt atggagttct
      121 ccagtaaact tcaataactt cgatcatcat ggaacaatga tgaatatttt ttatccatgg
      181 cttactcttt atccagcttt tttattttat aaaatgatgg gaaatcttgt tttaagttat
      241 aatatctact atttttttat cactttttta acaatggtcg tttcttattt ttctatgaaa
      301 caaataaaaa acaatcgtta tatttcgctg ttattttcga ttatttatac attttccgcc
      361 tatcgtgcca ttgacatatt ccatcgtgct tcattgggag aagctgtcgc tttaacattt
      421 ttgcctttga tactaatggg gtgttacgaa atttatatta gagattacca aaaatggtac
      481 tggttgtcta ttggcatgac gctagtagtg tatactcatt tactttctgt cgctatggtt
      541 tctgtattta ttggaggaac gttgtttctt tctttttatt tttgggacca aaaaatcgct
      601 agactcttaa gtcttttaaa agctaccgtc ctcacctttt ttttaagtgc gggattctta
      661 attcctttta ttcaacaatc tagagcacaa gaattgaaag tcccactggg aaaagaatta
      721 agtggtatgg ctcctagtga tatgctgaca catattttaa ataataacta taacaactat
      781 actattggat tattcttatt tctcggattg attggtgctt ttatttttat aaaaaaatta
      841 actgctgatg atttatttat tttctttctt ggtgtatttg ttcttttttg ttcaaccaat
      901 ttatttcctt ggcaactttt taaccataca ccagttaaga gtctacaatt tgtctggcgg
      961 ttaaatggtt tttcttcttt atttatagcc tatacgatga gtatagtgat ctattatatt
     1021 ttttctacta aaaataattc atggaaaata atggtcttta cgtttcttgc actaatattg
     1081 catctctcag gagtaagcaa ttctatccat gctaacaaag attcattaac acttatagca
     1141 cctgaagacg ctgttgctat agctgaaaat tacaatcata ctgattatgc aaacaaagag
     1201 tcaatctatc atcctgatat gattaataat gatcaatatt tactaggtaa ccaggaaata
     1261 aatccaacaa ctcattttat gagtaacaaa cttacaattg aattagataa ttcgaataat
     1321 aaaaatactg tcttgacgac acctatttat cgttataaag ggcaagtggc ttctattaat
     1381 ggcaaactag tacaaacaaa attatctaag tttggcacaa ctgaattaac tattcctcca
     1441 ggtataaata aagtcgttat tacttatcag tacacaaaac tagcaattgc ttcaagatat
     1501 ttatctatcg ttactttgat tttattttta ctttatcgat ttactttttc taaacaaaaa
     1561 caaccgagaa gagaagtaca acattctcac tagattttta tttaagaaag actttttagc
     1621 tgtacacata aacaggctaa aaagtctttt tttatatttc tttaatttgc ttcatacatt
     1681 atttaacatc gatatttata ctgtgaaaca tgcgtttcat ccattatcaa agtattttta
     1741 tcgataataa acttcatcca aaaacagacc ttgtgcaggg acggtttctc ctgcatgttg
     1801 ccgcactttg ctttcaaata tttcatcgat tgtttctatc ggcatggcac ctgtgccgat
     1861 ttctagtaat gtccccatga taatccgcac cattttatat aagaatccgt ctccgacaaa
     1921 agtaaaatga agcatttctc cctttcgttc gatagataat tcatcaatcg tacgcactgt
     1981 ggattttttt gattttttca atgcagaaaa accaatgaaa tcatgtgtac ccactaattt
     2041 ttcgcaagct gcattcagct tgtccatatc caactcttga gggaaataaa agctgtggtt
     2101 tcgctcaaat gctgaaggaa tcacgttgtt ccaaacataa tagctgtatt ttttgcccac
     2161 cgcattatat cttgaatgga agcgctcagg catttcttcg attcttttga tgacaatatc
     2221 ccgcggaagg taacgattca taaagtcgat catttctgcc agtccaattt ctgtgtttgt
     2281 tttaaaattt gctacttgtc ctcgcgcatg ggtgccagca tctgttcttc ctgatccaat
     2341 aatttctatc tcttcatttg tcatctgatg caatactttt tcgatttttc cttgaatggt
     2401 tttatcgtca tctccaagtc tttgccatcc tgaatatctt tttccgtcat attcgattgt
     2461 caacttgatg tttctcatat tttagtggct cctctgtctt ccatttggtc ttctctctac
     2521 ctttttaaga ttgtagcaca aaatatagct ccttgcttct ctaagacaga agctatacaa
     2581 gaaaactgtg gaaatatata tttcagaaaa acaaaaaaca gccaccggat gaagtatccg
     2641 ctgactgttc aatttgacgg ctcaagtttt actgtttgag atgcaagata ctcaacctgg
     2701 caatgggcac ataattaaag aaaaaactga ttctttggca aaaacctatt ttattcagta
     2761 atggccaaga atttatagaa ggaattcttg ccagtccata aatcgccgca aaaagcaaac
     2821 tgatcacaga cagtccaagg tataagtggc ttacgtccat ttgcccattt tttcgataga
     2881 tcaagaaaag aagaactgga aacaataaaa aaccactgta acctaaacct gatccgatat
     2941 tatggaccca tgtcgagaca ttccacgaag attcatgggt attgatactg aacaaacctg
     3001 taaagataca atcacctata ccatagaacg cgatggctgc tgtaactagg gtagctaaca
     3061 cacgagatgt actaacaaat gcagcataca atgcaggcaa tgacaggatg aaaaagatcc
     3121 ctgaaaccac agaccaaata agaaacgctt ttcttacagg actggaaacc tctccaaata
     3181 cactgatcac gctcttcatt tgatctaatt caggataata caatcctaat acgtacggag
     3241 tcaaaaaatc actcagcacg cctaataata aaaagaaaaa accaaattta cgtaaaaaat
     3301 ccatgatacg cctccaataa caattaccta tgcttttaat tgttattata aacgacttta
     3361 tcttgattta acagaaacct ctatttacac gaacatctat ataactcatt aaaaactcga
     3421 atcattttaa aaatactaaa tatctaacgt tcaacgttca cttaatccat ctaaacaatc
     3481 tttcaatttg attttttccc cacttctttt acgcgcgcct agctctccta ttgcagtaaa
     3541 caacagatca cttgacgaat ttaaggcagt ttccattgaa tcctggataa caccgataat
     3601 aaatccaata ccgacgattt gcatcgcgac atcattagag ataccaaaca aactacatgc
     3661 taatggaatc aataacagtg aaccgcccgc aacacctgaa gctccacaag cagctaaagc
     3721 agacaaaata cataaaagga ctttcaaaac aaaaggtgct tcaatcccta aagaatgtac
     3781 agcagtcaac gtcatcaagg aaatggtaat cgcggcaccg cccatattga tcgtagcacc
     3841 taacgggatc gatatggaat aagactcttt tgtcaatttc aatctttcag ccaacatcat
     3901 gtttatcggg atatttgcgg cagaacttcg ggtaaagaaa gcgggaagtg cgctttcttt
     3961 caaacaaaag aaaatcaatg gatagggatt ttctttaata aacaaaaacg tgaggaatgg
     4021 gtacacaact aatgcgacaa acagcatcgt accgatcaaa acaagtaaaa gctgcagata
     4081 ctctgccaat ccagcgattc cagtcgtagc aattgattga taaaccaatc ctaaaatccc
     4141 tatcggagca aatgcaataa tcacttgtac gacttgtgac aacgctatag atatctgatc
     4201 caccaactct tttgtcgatt ctgaacgtgc tcgaaaagct agtcccaaca atacagccca
     4261 gaataagacg ccaaggtaat tcgcatcaat aagagattga acaggatttt gtgtgacatt
     4321 cgtcaacata gtagataaaa cactttccaa actttgcggt gctttttcag atactgtttc
     4381 ttgtaaagta ataggaacag taaaaattga acttacaata actgctacaa gtgcagaaag
     4441 caatgttccc actaaatata agatcaaaat aggcttcacg aatgtcttat tcccaatttt
     4501 atgcttggca attgacgcca tcgtcaagaa aaaaaccaga ataggtgcga ttgccttcaa
     4561 gcaactgaca aacaacgttc ccaatattga aataaatgtc catgtgggga aaaagatacc
     4621 aaacaatatt ccgactaaaa taccaacaac aatacgttga actaacgaca tatccaaaaa
     4681 cttctctaac aaatgaccac cctttcacaa ttttaacaat cattgtatca ttttatgcaa
     4741 agaaactaaa ggtataattt tctaaaaatt ctaacaatac aaacaaccat ttcttgctaa
     4801 ttttgtactt tttctgtcta cacgttgttt tttgagatat tctaagttag gatgttatta
     4861 atgggcaact tatcaggaaa cgttttctta tgtttttgtc caattttttt gtgaggtgtt
     4921 tattcttgaa aaaaataaag aaggagagtt atggcttcct gacaatcttt gtcgcttgta
     4981 taatgatggt tttacagcgt atattgattt cagcagtcac tattcctaca tatgcaacaa
     5041 accctggtcg attctacatc tatacatttt tacaaacttc cgttgttatt ggtgcaaatt
     5101 tgatcccact ttacatgggt taccaatatc aaaaaatcgc ccgtttaaaa gttttacatt
     5161 atttaagtcg ttttgccttt atattcctaa tagccacact agtttgcaat atcttttttt
     5221 acgtacaagc tggcagcttg aatattcgtg attattggct tatcctaaca cctatcagtc
     5281 agaattactt tacttatgct gtttcttgta ctttgctttt ttgtagtatt ccttatacag
     5341 ttggattttt aaataaatta tcgaaaacaa caattaaata tctactatta tttttaacaa
     5401 ttggattagt tggctgctct actctattta ataaagaccc gtttgcatta cagaatggac
     5461 agagtatttt atggattttt tatttatttt taatgggtta tggtttgaaa gaatggaact
     5521 ggaaagaaaa ggtccgctat aagcttcctc aagtatttat cagcttgatt gttttattcg
     5581 gtttgattat tttgatgaca caaatttcac tcatcatcag aggaaataca gatacagcgc
     5641 tgaggttttc taaaccatat tctctcttcg caatgtatta tactttctct tcgtttttat
     5701 ttttagaaat gctatctgat aaattaggta taaaaggaag aggaaatttt cttgctgtat
     5761 ttttgattgt cactcaagtt atatgtaatt tccctttagt ttcttatcgc gtatctacat
     5821 ttatgaaacg tgaatttcca aactcaggga aggcatggct gataaatatc gggcagtttt
     5881 ttatttgata tttattagca gtgtccctcc tagttatctt tttattttat atacaaaaaa
     5941 tccgtttcat tcatcgtttc atcgaatact tcgcttttga ttcgattgaa caattaactc
     6001 agcgttggaa aaaaagaatt cattggcttt ttgttcataa aagttatttt ttagttgcag
     6061 cattttttta ctgctttacc ttcgtgcaga ttttcgtttt agaaccaaaa gaaggttgga
     6121 aagaaactat ccaggttgcc ttaaaaatat ttactcagcg acaagcaccc atgatcctga
     6181 caatcatcat tattatggga tttttcttct tactattgtt gatttccaat cgtttttggt
     6241 acgccttgac tgctaccttg atcattaact tactgctcac tatttcgaca gtaatcaaaa
     6301 tggaaatgcg ggaagagcct gtctttcctt cagatttaaa aatgctgacc ggattgtcag
     6361 aactattatc gatggttagc ccagtcttat taatcgtggg cggattgatc cttctcctat
     6421 tattgattac tagtattatt gtacaacgta gactacaaaa acagtattcc ttgaagattc
     6481 attggaaacg tcgatttatc agtatcgctg tattattagg atgtttttcg ggtgtctttt
     6541 tcattaatca caaaaattcg ccttctttct tgctgtttaa tttatttaaa gtaaacaaga
     6601 ctttctttaa ccagcaaggc gccgtaaaag ataacggacc agtcattcaa ttcctaaaca
     6661 atatcgacat taaaatcatg gaagaacctg ctggctattc gaaagaaaaa attaatggaa
     6721 taatgaaaaa gtacaacaca aaggcagatg aaatcaacca aacaagaagt gactggttaa
     6781 acaatcagac gatcgttctc aatttaagtg aaagcttcag tgatcctgca cgtgtcccta
     6841 atctgacagt acctacagac cctattcctt acatcaaaag cattataaca gaaggaacag
     6901 gaggattgat gttatccgtc ggttatggtg gcggaacagc taacatggaa tgggaaggac
     6961 tgacaggctt gagtatcagt aacctgtctg cttcgttagt tacaccttat acccagctag
     7021 tggagcgtca gaaaataagt ccaaatatta ccgatctatt cgatgaaaaa atagccatcc
     7081 atccttttac agctgctctt tatcgcagaa aacaagtgtt tgataaattt ggttttgaca
     7141 ggttctatta tttagacagt ccggataaat taacttatac ggataaaata gaacaaagcc
     7201 cacgcatatc ggatgattca gcatacaaag aaaccttgaa agctttacaa agcaatacgg
     7261 aaacgtcaca attcatccaa ttatccacta tgcaaaacca tatgccttat gatgattact
     7321 atagtgaatt gaactacact gctgaaggaa ctgctgtcat tcctaataga agaaaagaat
     7381 tacaaactta tatgcaaggc cttcactata ccgatacagc agtaaaaaaa ttcatccaag
     7441 aaatcgacaa aatcgataaa ccgattacct ttgtttttta tggtgaccat ttacccgcaa
     7501 tctatagtgg gaatgattcg aaaaaatatg gattagaaca gcaccagaca gattatttca
     7561 tttataacaa caagtatagt cgtgaacaag cagctacgtt aaataaagaa gttgttgctc
     7621 ctaataactt tggtgctatg gctcttgagc atgcgaatat caaagtaact ccttactatg
     7681 cgttgttgac agaagtatct aatcatctac ctgcattaac aattgatcct acagaaagct
     7741 tgtctaatcg ttttaatgga aaacaggtct ttgttacaga ggatagtcaa attatccaag
     7801 aaaaaaattt aactgagaaa caaaaagaaa ttctagaaga ttatcgtctg atacaatatg
     7861 atcttgttgc aggtaaccag tacagtgcaa catgggcgac acaaaaaata aaagactaac
     7921 aaataaactg agcaaggggt tgtgataaaa gacatgtcac aaccccttgt ttatctcatt
     7981 aatttgcttg tccataaacg atttgattaa agccattttt caattcatta ttcgctgtct
     8041 cgttgaagta aagataatta tcgggattcc cctgattttg ttgtgtcacc aatacttttg
     8101 gctgattttg ctgaaacccg aatagataga cgtgcttttt cagatatggt tctgtctctg
     8161 catcggaata gacggctgtg atttgatagt ctgcttgacc tgtcccactt tctgaccatt
     8221 ctgcagaaac aggcactcca ccaatagcca tcttccactc cccgttcaaa atagcttgag
     8281 ggactttaag accatataag tccacactca tttgattcgt ataacttttg tattgttgtc
     8341 ccattgtttg gccccatttg gtcataaatg cctctaattg tgcggatttg tctgcattcc
     8401 acagtgtttg ttcaacttgt tcgctgcttt ctgaagagga agatgaagcc tctacagaag
     8461 aacttgaagt agatgtttga gttgtttcag tcgttttgga agactctgac gaagtagagg
     8521 tatctgctgt ttctgtactt gctgcagttg acttgctctc tgtgctagtt gttgtagtag
     8581 ttgcaactgt tgctttcgct gtttcttttg ttgattcttt tttcgattca ttcccgttgg
     8641 tacaaccagc taacaaaaaa gctagaccaa ttgttactaa aatccttttt ttcaattaaa
     8701 aaactccttt cgattactcc cacttcttta ttctatcaaa taagcaatgt gattttattg
     8761 atttttcgta ttttctcatc aatctttcaa atcttctaaa aactgagaag cacccgctga
     8821 tgttgtctgt aatacatcag cgggtgcttc ttttatttta atcttcgtga ttttccaatt
     8881 tacctcacgc tttatatttg tatttgaaga aacatagact tttagtcatc atcgtcttcc
     8941 tcatccaaat cttgtcggac agtttttcct tggagtcttt gtacttggta gatgatttct
     9001 ttattgaaac ttaaaagttc aacaagcaat tgttgcaagc catatttttc ttctttttca
     9061 gctaatttga ttgcccgtgt gataaagaca ttttggaaat caaaatcttt tgcaaagttt
     9121 tccaacattg ttgaggctgg taaatatttg ttttgaccat gttcgtaaat ttttccataa
     9181 cgcatgactt cttctaaagt agacggaacg atttcatttt caatcactaa ctcatgagag
     9241 attttattga accatctcgc ttcttgatca gcatattctg aaaaaagggt acttaattct
     9301 tgcgaagatg tacccaaaac atacaattgt gcttggatca atttcatttc ttctaattta
     9361 aaatttgcta atacatgccc caccatggcg ccagcagttg gtttatgatg gtcgatatct
     9421 gcttgttttg tttcttgaac aaatttttct tccactatat tcattgattt acgcctcttt
     9481 tacttttgct gattggactt cataacctaa tttttccaca actgcagcta attgttcagg
     9541 gtggacttca tctggatcaa agtttgcttt gattttgctc gcattaaaca aaactttcgt
     9601 attgcttacc ccaggctgtt gagaaaccgc atgttcgatt ttttgcatac agcttggaca
     9661 agctagtgta tctaattgca taataatttt tttcatcgta gttcttcctt cctttttctt
     9721 gatagtctta ttctagatta cagcttccat aaaaaaattg attcttatca agttttacga
     9781 tttttcttct ttgctttcgt gtccaaaata caataaacgc atcgcgttaa agataacgat
     9841 caagatacta gcttcgtgaa cgaacatccc actagccatg taaataaacc cggcaaataa
     9901 gccgttgagt aaaaataaga cgacaccaat ggcaatcact acattttcac gggtatttaa
     9961 aacagttttc ttagccaagc ggtaggcatg aaccaacgat tcaaaattag attgcatcaa
    10021 gacaacgtca ctcgtttcga tagcaacatc cgttcctgat cccatagcaa tcccgatatc
    10081 agccgcagcc aaagagggag aatcattgat tccatctcca ataaaagcaa cgcgcaatcc
    10141 ttgttcttgg tattttttca caaattgaac cttttcgtct ggtaataatt cagcatggac
    10201 ttcatcaatg cctaacatat cagctacata atcagcagtc atctgattat cacctgtcag
    10261 catcactaac tgttttgctc ctgcttgttt caacttagct aattgttcag cgacttctgg
    10321 tcgaatcaca tctgacacac ctatgatttg agtgattttt ttgtcgattg ctacaataat
    10381 gaccgagctg cctgtttgtt gtaattgatg taaatcttgt acttgctttg gtgtgagaga
    10441 aagtttgtga gcggcaatca atttggtgtt gcccgcacaa actgtatgac catcaatctc
    10501 agctgtgatt ccttgtccct tgattgtctg attatctttg actgctaatt gttgaaaatc
    10561 aatgtctttc tcctgagcat aatggatcac ggcgcgcccc agaggatgat cggataacgt
    10621 ttctaatcga gccactaaag ctaagctgtc cccttcatcc tctgtatagt tcttgatagt
    10681 ggatacagca gtagttcctt tggtcaatgt accagtttta tcaaagacaa aagtatcgat
    10741 tttggaaaaa gtatccattg cttcaccgcc tttgactaag atgccccgtt tcgctccatt
    10801 accgattcct gctacattcg aaacaggcgc accaatgact aaagcgccag gacaaccaag
    10861 aaccaaaaca gtgattgcta aacgaaaatc acgactaatc agaccaacaa ccaacgcaat
    10921 aagtaaaacg gttggagtat aatactgaga aaatcgatca atgaattttt ccgcatggga
    10981 tttggtatct tgtgcttctt ctactaattc gataattttg gcaaaggtcg tatcatctcc
    11041 gaccttagtt gcttcaatcg tcacgtagcc atctgctaaa atcgttccag agaagacttc
    11101 atctccactg attttgacga cttgtcgtga ttcaccagtc actgcggctt catccgcata
    11161 ccctttcccg tcaatgactc gtccgtccac aggaatagct ccaccagttt ttactaagac
    11221 gatatcgcct tcttccacgt catcgatttc cacttcttca gtcgttccat cttcttggac
    11281 aacactagca gtagatgggg ccatctcggt caaagccttg attgactccc gcgttttggt
    11341 caatgttttt tgttccagat agctaccaaa caaaaacaag aatgtaacga tagctgactc
    11401 cttgtattct ccgatcataa aggcaccaag cacagcaata gtaacgagta aatcaattga
    11461 gataactttg actttcactg cctgatacgc atggatcata ataggtatga ccgcaataat
    11521 agaagctatg gctagagcaa tgttatagaa caagacaaaa ccagtaaatt ttccgataaa
    11581 accaaaaata atcaacacag ctgataaggc ggtgatatga tttcgatgct cttggatcca
    11641 cttttgtaat ttcatctttc tccctctttt cctttcattc ttacgagact aagtatagat
    11701 acttatccat cataaaaaat tgaccactat caagtttagt caaaaaggcg gttttctttt
    11761 ggtagaatac actacaaaaa atagttagca agaagactca ctaaaagtga tcttgccaac
    11821 tattgtcgtt tctatcgaaa ttttcattga atgattttcc tgtacagcat acatcggaga
    11881 tctatttctt tatctaagat ttttatcttg ctaatttttt cctttagtct ttcaaatcca
    11941 cgtcgttcat agaattgata cgtacagcca ctatctgtaa aaaggaatat ttcttttcct
    12001 ttctctcttt tttcaaattc tgttagcaac ttggtaccga tgccttttgt tttcaaatca
    12061 ggatttgcag ctaaaaaaat gatctctcca tctggcgaat gcttctttaa atatgctgca
    12121 agcatttgtt catttgctgt ctcgtaagct cctttacttt ctttagcaaa tatattttgt
    12181 aaaaaatcga atatcttaac atatatttgc tgtccaaaag aacgatgttt ctttttttct
    12241 ccttttatct ccgccagcaa aacacctgcc aactcgtcct ctgcatacaa agcaagaatt
    12301 tgagttgctc gattcaactc catatacaaa aaatatcttc cgtaaagatt cagcagaagt
    12361 tcattttcca tatatctctc aaagttcatt ccttcaacag caaaatagat agcttttttg
    12421 taatctttct tttctaaggg tttaaaagtt agtttcatca tttcgcctcc tcaacttcaa
    12481 tgattgtgtt atacttacat tataatagac acagtgtcta ctatctataa tcaatcatct
    12541 ataagtttgt cgattaagcg acagctttgc tttgatatgt cggataagga gataggtaaa
    12601 tgaaaaagac agatagacgc gtcaaaaaaa cagaaaaagc tttggctgaa acactatcca
    12661 cattattagt aaataaaaag attcaagcaa tcactatacg cgaactaaca gaaacggcag
    12721 atgtacatcg ttcgactttt tatacacatt ataaggatat ctatgatcta tatgatcaat
    12781 tagaaagcaa cttttttcgg gatctaaatg aaattctctc ttatgatcca gcccatagct
    12841 atgaggaact atacactcgc ttgattgact atgtttacgc aaatactttt ttatatcgct
    12901 tatttatcct caaccatggt gatatttcgt ttcagaagaa agttaaacaa gttattgaag
    12961 aaaactatct gaacatatgg ctatatgaag acaagaaaac gattatcaca gaagaaatga
    13021 gattctttac tacgtaccac gtccaaggtt gtctatccgt tctcaatcgg tgggtagaaa
    13081 gcaattacaa gtactccaaa aaagaattat tagaactctt acagaaactg aatgatcaca
    13141 tagagaaaat catgccttga ataaaaacat actaagctta gttaagaaga accgtaacct
    13201 tctcgtataa caactgaatc ttatttagcc aaattgttta ctgaagattt tttgagtaaa
    13261 atttctataa ttcctactta ttgaaaagta gtttgtagtg ctgcttctcc ctctctaagt
    13321 gatatatctt gttgtaccaa tcaaattcac ctcatcatta ataaattact tttaggctaa
    13381 tttattacaa cttataggtt gaagctgtaa ccaatttata gtcatattat atttttatca
    13441 tccagtttat tgaattcttc tcgtttacct caattcattt tcaaggggaa tcagctcatc
    13501 atctaacatt ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg gtttcgcttc
    13561 tggatgtttg gctttatatc cttcgtaatc cttactgttg ataattgctt tcatattagg
    13621 aattggatct tcacctggca tatattgcag attagaagca tgtaattttt tctcagaaac
    13681 aaaccactca ttggaaaaga catgaatcaa atcatcactt acttgtgtaa aatagcgacg
    13741 tttaatgaca aagatatctt cattgctatc tagtgtacct tcatttcttt gtgcttaaat
    13801 tcaaagataa ttttctcaat aaagattttt gattccagta agattccttt actcctttta
    13861 caacatagcg ttgtcctttg ttttgaaaca tcttcttccc ctctttgcca ttaaaaatag
    13921 cccaattctc tataaagaag attaagcttc attattataa tataaagttt aaatttggtt
    13981 tattacttac tttttcttat tctctagtaa gctaatattg aaaaaaatgt aatcagatca
    14041 ttatttttat atgttgtaac ttcctgataa gaaaactaat taaaagaaag aggtaactac
    14101 ataaactaat taaaaaagct aacttcatag ttggaggata aatggaagaa acttcaatga
    14161 ttgtttctcc ttgtgttcct tcaatcgaca ctagtcctat tatcaatctc tcttttttgt
    14221 ccatttactt ttgcgactgt tccccaataa tcaaatacag gaagcgttat tttttgtcct
    14281 ttagattcat gtactgtata gactattttc gaatccatca cttgataagg catagtatgc
    14341 cattccttgc caattcttac ttcatgattc tgcaaatgtt ctttgtattg taatgcttcc
    14401 gtgggtgcat agtcataaag tcctccggga aggagtgtcg tgtaacgaat aagattttct
    14461 tgattcatta ccaagtgatc tctctgagtg acaactttat gttcatagtt tgcttttgtt
    14521 gcagcagata cagccaacag taataaaaca attgccgtta tattaactac ttttctttgc
    14581 ttagctgatg tgctcttcat gaaaacgaac gataaaatca acgagccaaa cagtacttga
    14641 aagcctaaga ttcgccaagg aaattgaatc atttgaatgg gtgtattttg taataaagac
    14701 catggaaaaa atttggtgga taagagccaa gagactaccg cactaaggta aatcactttt
    14761 tctttgcgat taaatccttt aaaaaagaag agtggacaga tcaaagaaat aataccaatg
    14821 attcctaccg taaaggttgt caggtcatta ttcatcgcac caatcaaact atccgagaca
    14881 tttaatgctt ggtgttgtaa ggtcgtttta tctggctcat taatcgattg aaccatgatt
    14941 tgtcggaaca tcggatacca aaagtatgct gttaaaagaa tcgtcattcc tactgccttt
    15001 gacaactcca caatcaaccg gatgctttct ttttttcttg tttcacttac tacaaagccg
    15061 cctaagacta gtaagccaac ggctattaca ctcataaaag tggaaagaac atgtgtataa
    15121 atgagtaagc tcatactgac tgctaatgga attgcacatg gctttttctc ataaatcagt
    15181 gcgtaagcat aatacataac tatcggtata accgtaagtg caatcgcttc agcaatagca
    15241 gaacgatgat aaatatcaat cgtacggtac ccataaaatg tatataaaca gctaaatata
    15301 aaagccgcat cgattctttt taaaaatttt agtccataat gaaacatcag catgatagta
    15361 caaacattca atagccaaac atataagata taactaacga ttaaattatt acttatccaa
    15421 taaaaaatga ctgccggaaa aaaggtcaag tagggataaa aataattcac tccgctccca
    15481 taatgattaa acgtcgtaaa attgattgga ccagaaaaaa ttgaagataa ccctttgact
    15541 ctagcaaaat ggaaggcaat atcccccgat gtagaaatag tatgaacatt tctaaaataa
    15601 ttgattgaat aaagtccagc aataattgct aggagaatgg acaatattat ttttttcttt
    15661 acttgcatta cttaattata tactcctttc atggattgct ttatctagta taacttaaat
    15721 tttaaataaa tcgttattta tttttactct cacttaaaaa agcactaaaa ttataagaga
    15781 gggaggtaat ggtctgaaca ccaagaattg ttccaacatc gaattgctta actaatgagt
    15841 tcaaagctta attatgtggc tcttatattt accgagcgtg gattgcgatc tctttagcga
    15901 aacaaacatc tttcataaaa aaaacagcca tatccgcttt cttggatatg actatttact
    15961 actactttac tatctttcat tcttcttgtc tcacttatga taaggcgctg cgtatcatga
    16021 gtgaatgata ttaggaaaac ttcataaaat aaatctttta tacatttcca cttctagatg
    16081 gtcattcatg ttagaagaat caaactattt ttttactgtc gtaataatat taaaacacta
    16141 gaacaaatca attaaattcc ataaaatata aataatagac ctagcatcat ctggtataaa
    16201 atggatgcta ggtccgttat atttactcaa aattgattct acgaatagat cttcttatca
    16261 tttctatttt agtagtcatg cgttgttgtt cttctaaagg tggtaatggt ataagtagtt
    16321 tagataaatt tgtaccattt acatttggtt gcccagttcc gtaagaaatt ttttctaata
    16381 acttccaata taatggcgag tctaagaagc agtctacgta ttctgataat aatttctcaa
    16441 ctaacctaat tcgaatcaag taagaagcaa aaacagattc ctcacttatt tccttaacca
    16501 agaaactttt tcccatagtt cctcctgtcc ttgctatcaa aatgtcattt tcttccaatc
    16561 ttaaatcaac tagtttagaa ttactaatat cacagtaagg cacactggac caatttacac
    16621 gtccctcttg tatatctgtg attctcaaaa acttaacatt gccttgtttt ttcgccgaat
    16681 ctgtatagcc atattgaatc ttagtagaaa tacttcctaa ataaacccat agtcgaacat
    16741 cgctagatga tttttcttgt cgttctttat gaacaacagt gatcatcttg agttgtccga
    16801 ctatttttta gttgcagcaa tctttttggg acgcttattt gcatgaagca acgttgctaa
    16861 gtctagtaat aaaacattgg ctaacacagg attggctttc tcgcgatacg ctgcggcgcc
    16921 actttcaaac aatactgcta gacgatcttc tacaacaacg atttgttcca aatacggtgg
    16981 aaatttgatt cgttttagga tccggctagg agagtcccgc tcggacacta agagttccga
    17041 ggtataagga ccataagaac gactggctaa gagataatgg ccataaaaag ccacaccttg
    17101 aatcttatca gatgtttttc tgctcattgt cacggttggt tggattttgt ctttgcggcg
    17161 aattgctgtt ttcatttcag ctatcgtcgg caatagatag aaagtcaact gtccttcacc
    17221 ttttagtgtg aagttgccag ataccagttg atcattgttt tgagtcaaat acgatgcttg
    17281 tggaatctct gagagatcag ttgtcagcca ataagcgatt ggctgtcccg tctcttcaga
    17341 tgtatctgct aagatatcga tcaaacgtaa agcggctact cgtccagtag cagatcctgt
    17401 gattgtcaac cataaaagtt cccgttttgt atcatatact aagcccccta catgtggccg
    17461 tttaggtaaa atcagcagtt tgatcggctg tcctgtttct ctatccagta catgaataat
    17521 agaatgatgc tgatggtcat agcagtaggc agaaataaac acaaaatctt gagaaaaagc
    17581 caacccttgc ggcgtcatcc attgacaaac ctcattttcg ccggtttccg gcaccaccat
    17641 tcttgtttct gttaatccag gaatgttata ttgttttgct tcagcctttt cccaagctgc
    17701 ttgaacaata ggataccgtt ttaacttttt ttcaatatcc tgatgtatcc gttgctgctt
    17761 ccaatgctta aactttagga catagtaaac aagaaggcca atgaccagca acactaaaat
    17821 cattattgct acccgctgtg tcattttttt gcctccttgt tctattcgat caatcgtcca
    17881 tataccgcta ttaattaccg taactacatg tttcttttac tagcgtttct ttataggatc
    17941 tttcataatc tcatttatag taacattaag aatcccttat tctactatag tcacttccac
    18001 atcaatattg cctcttgttg ctttagagta tgggcaaaat gcgtgggctt cttctgcgat
    18061 tttctctgct tggtccttct caagtcctgg caaagaaact tctaaaacga ccccgatttg
    18121 gtagccccca tcttctaatt gaaataaaga tacccgtgct ttgacttcag aatcagactc
    18181 aagattattt tcttccagca tgtgttgaac cgcgccgttg aagcaagatg cgtatccagc
    18241 cgcaaaaagt tgttccggat ttgtcccttc actatggatt cctgggcggt cgatcttcat
    18301 gtgcatactt ccgtttggtg cctcaacttc gccatcacgg cctccacgat tgatcattgc
    18361 tgtttcgtac atttttttca ttgagatcat cctttctttc gtcgttctat attaagtgta
    18421 acgagtattt tttaatctga caaaggtaat gcttgcattc ggcgacatca gttagacaac
    18481 atcgatgatc agtgatagaa gcagtttatc tatacaaaaa aagaagggtt ctatgatatt
    18541 tggtgtcctt actcgccttg agtaacaaca ccaaatatca tagaaccact taatcagctt
    18601 cacactgctt tttctacttt cgtagttgaa aaaaacttaa acaaatcatc ggcaaatagt
    18661 taaagaaaaa acttaatcgt tgaaagaagc ccaattgctt aaagaaggca aactgactaa
    18721 tcgacggaat ccgtgctaat ccgtaaagac ctgcaattag taaactaata actgttaaaa
    18781 caagataaaa atggctaagc gtaccttgac cactttgacg ataaagcaga acaagtaaca
    18841 atgggaaaag tagaaagcct gcatagccta gcccagaacc tatattatgg atccaagtcg
    18901 atagattaca agagctttca ttcgtattga tactgaataa gccagtaaaa atacaatcac
    18961 ctatcccata taaaccaact gttgcaacaa caagtattgc aagtgttttt gatgttccaa
    19021 caaataggtg atacaacgca ggcaacgaaa gaacaaaaaa aagtccagat acaactgacc
    19081 agaccaagaa agcacgtctg actggactat ctacgtcacc aaacacacta attacggtag
    19141 tcatttgatt cagtttggga taaaaaaggc caaggatata cggtgtcgat aaatcactca
    19201 tcacacctaa taataaaaaa taaaaaccat atttttgcaa aaaaatca
//
LOCUS       DANKAZ010000047        19202 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000047 DANKAZ010000000
VERSION     DANKAZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            102..2543
                     /locus_tag="PWY67_001618"
     CDS             102..2543
                     /locus_tag="PWY67_001618"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL2057891.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="PWY67_001619"
     CDS             complement(2873..3577)
                     /locus_tag="PWY67_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057892.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="PWY67_001620"
     CDS             complement(3764..4009)
                     /locus_tag="PWY67_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057893.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="PWY67_001621"
     CDS             complement(4026..4877)
                     /locus_tag="PWY67_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2057894.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="PWY67_001622"
     CDS             5043..6047
                     /locus_tag="PWY67_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL2057895.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6288..6644)
                     /locus_tag="PWY67_001623"
     CDS             complement(6288..6644)
                     /locus_tag="PWY67_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2057896.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7012..8199
                     /gene="tuf"
                     /locus_tag="PWY67_001624"
     CDS             7012..8199
                     /gene="tuf"
                     /locus_tag="PWY67_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2057897.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="PWY67_001625"
     CDS             complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="PWY67_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2057898.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..11765)
                     /locus_tag="PWY67_001626"
     CDS             complement(11004..11765)
                     /locus_tag="PWY67_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2057899.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12043..12564
                     /locus_tag="PWY67_001627"
     CDS             12043..12564
                     /locus_tag="PWY67_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL2057900.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12623..13483
                     /locus_tag="PWY67_001628"
     CDS             12623..13483
                     /locus_tag="PWY67_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL2057901.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13668..15983
                     /locus_tag="PWY67_001629"
     CDS             13668..15983
                     /locus_tag="PWY67_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2057902.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16165..18006
                     /locus_tag="PWY67_001630"
     CDS             16165..18006
                     /locus_tag="PWY67_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL2057903.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18030..19139
                     /gene="rpoD"
                     /locus_tag="PWY67_001631"
     CDS             18030..19139
                     /gene="rpoD"
                     /locus_tag="PWY67_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL2057904.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         6228 a         3392 c         4028 g         5554 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     6181 ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg gagttaaacg
     6241 cttctgtccc agcctctttt tatctaataa aattttaaga ccatttacta attatcgatt
     6301 ttttctgccg catttttagg cacttcagaa gcctttattt cttcccagct taatacacct
     6361 ttacgaggat tgactttgag tttaagatac gcattcatct ttaatggatg ttctctttct
     6421 tcgcgaagcg tcacctgttt tttttcacca tctttattat aagccgcttg cttgtaggta
     6481 taagtcgtga atttctctcc gttatctgct tgggtagctg acatttcgcc attcgtcgtg
     6541 atttttgtat aataagcttc gccgccgtaa aaataatgat aagctgcaaa acttccgccg
     6601 agaaatatgc ctaaaacgac aagtacgcca attaattttt tcatgtactt cacctctttc
     6661 cttattatta acgaaataga agatttcgtc tattgaaagt tgttggcaac agctgaattt
     6721 taaacttttc ctcattttgt tcttattgct tcaaaagaaa gttaaggact atcttgaagt
     6781 ttttctcaaa aaacacattt ttttatttat aaaaggtact tttttcgcgt ataagacaga
     6841 cgagtgctct tagttgactt tgaccaatat tgacgtataa taagcttagt tcgaatctta
     6901 caaaaagtaa gtcggacgta ttctttttga tttagtcaaa aaaagactat aataaaaagg
     6961 tgagagctga aatttttcag tgatctatat tatttaggag gtttgtaaat tatggcaaaa
     7021 gaacattatg acagaagtaa accacacgtt aacattggta cgattggaca cgttgaccat
     7081 gggaaaacta ctttaactgc tgcgatcact acagtattag gcaaaaaagg cctagcaaat
     7141 cctcaagact atgctagtat tgatgcagct ccagaagaac gtgaacgtgg gattacgatc
     7201 aataccgcac acgtagaata tgaaacagaa aaacgccact acgcgcatat tgatgcacca
     7261 ggacacgcgg actatgtgaa aaacatgatt acaggggcag cacaaatgga tggtgcaatc
     7321 ttagttgttt ctgcaactga cggtccgatg cctcaaacac gtgaacacat tttattgtca
     7381 cgccaagttg gtgtaaaata cctgattgtt ttcttgaaca aagttgattt agtcgatgat
     7441 gaagaattga tcgatttggt agaaatggaa gttcgcgagt tattgagcga atatggtttc
     7501 ccaggcgatg acactcctgt gatcaaaggt tccgcattaa aagcattgca aggcgatcca
     7561 gatgctgaag ctgctattat ggaattgatg gatacagtag atgaatatat cccaacacca
     7621 gaacgtgata cagataaacc attactattg ccagtggaag acgtcttctc aattacaggt
     7681 cgaggaactg ttgcctcagg tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta
     7741 gagatcgtag ggatcaaacc tgaaacacaa aaagcagttg taacaggtgt agaaatgttc
     7801 cgtaaaacgt tagattacgg ggaagctggg gataacgtag gcgtgttgtt acgggggatc
     7861 caacgtgacg atatcgaacg tggacaagta cttgctaaac caggttccat tactccacat
     7921 acaaaattca aagcagaagt gtacgtgttg acaaaagaag aaggtggacg tcatactcca
     7981 ttcttcaaca actatcgtcc acagttctac ttccgcacaa ctgatgttac aggaacaatc
     8041 acattgccag aagatacaga aatggtcatg cctggtgaca acgtgacgat tgatgttgac
     8101 ttgatccatc caatcgctgt tgaaaacgga acgacattct caattcgtga aggcggacgg
     8161 acagtaggtt caggtatcgt tacagagatc gaagcataag aaacgcgact cgaatatttt
     8221 ggtaatgaaa aatgtttaaa ataaaagagc ggtccaaaca ggatttctgt ttggaccgct
     8281 ctttgttctt caattgtata agaagagatt gtagaagcat gaactttcgt tttacttcag
     8341 tctcttttat tcatcatgag agtgttcatc tatcggtatg cagtttttaa tactttcttc
     8401 gtatccattt tttcaaacca cttattctac cttgtgtctg ctcaactggc gcattatcaa
     8461 taatcagcaa agcaatcata acatatgcaa tccaactatc tctagaatat agagtataac
     8521 gtgagaccca gtttgtagcg tacttgcttt tatattctct taatccatgg aaagaataaa
     8581 aatgagaacc aaactcgtaa accaagtatg caatcctttc ctgtaaaaaa ctctttctgg
     8641 aagttccgac atttgaaaga ggggccatcc ctaaattgaa ccattggata tgttcttctt
     8701 tcatataggc aaacaaatgc aagaacaaga aatccatact gccagaaggt gcttttttag
     8761 aatcatatcg catcagatca atcgttccta ctttattgtt cgtgtaagta ggcatgatcg
     8821 ttgcaaaagc gacaatttct tcttgggtat ttttgacaac tgcaataggc gctctttgta
     8881 gataatcttc agagaaaaag cctaaagaaa accctttttc ctttcgtgaa cccaaccaat
     8941 tatcagagat atttttgaaa gtactcattt gctcagtaga aaatggtggt tgtaagacat
     9001 caaatgtaaa tccttctcgt tcgatccgat tcaagacagc acgagtgccc ttcatttttt
     9061 tccctgaagt tgtaaaacta ttcaggtcta cataagcttc ttcccccatc ttaatgaaat
     9121 cgtagccgaa ttcatgcaaa atcataacaa tctcttcgct tgtttcgtag aaaactggta
     9181 aatagcatag tcgatctgtt tcttcgataa aagcttcgat tgcttctgga aaatcttctt
     9241 tcttaccaga aggatcaccc attacaatac atttattatt gaagcaagcg aattggagaa
     9301 gaacagttgg ttcaccgtct ttttcatagg caaacatccg cttatctttt aagaagatca
     9361 attgactatc ggagtttcct ccataagttg taaggatctt cagggccttt tcttcattaa
     9421 aggcttcgcc aatctgtttc ttttctcctt gaaggaaatg aacaaataga acgatcatga
     9481 aactaacagc gatgatggcc aacaatcccg aaaaccaaat cttttcagaa ggaaataaga
     9541 aaaaggaaat aaaatggtga cgccgatgag ggaaatccgg cagattgtat accccaataa
     9601 cgatgtaaag caaggacagt gcaccaataa tgattccatc gatcgtacgc cactcccatg
     9661 aatagatcaa ttgttcccga aataattcgt ttttagaagc aagaatgatc agaagcagaa
     9721 tagacaagaa aaccaccggt gtgaaactaa aatcacttaa taaaacataa agtaaagcta
     9781 gaacaatcaa gaagattgta ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc
     9841 ccataatgat taaaagaaat cccaatagta tactaggaaa ttggataata aaatgaaatt
     9901 tcaatgggtt caaactatga agccagcgaa actctgtaaa agcttgcggg atagtggcag
     9961 aaagtactag catgattcca gaaaagtata ataagacaac cacgatttta tgcgcaattt
    10021 ctgttgctaa ttgtttaggg ataccagaat aacgctgatc aaaagtggaa ccgatatttt
    10081 tgaagaaaaa gacgattcca atcaaaaacg gaatgatgta gtagaacagg cgatagagca
    10141 agatccacac aacgactatt tctcttggca caccaatagc ggacaacccg ataatcatca
    10201 ttacatcgaa actcccaatt tctcctggaa tcatagacac gattcctatc actgaagcag
    10261 caatgaataa tggaattgtt tgccacagat caatccggat ttccattaaa taaccaacag
    10321 aaaggaaact aaacaggaca ccggtccatt ccaacaatga aactagcaat agctgtcctt
    10381 gagttttaag tgaggtacca ccaaggaatc ctttcttttt gaaagtagta aataaaaaga
    10441 cgacaggaaa atatagaccg ccgccgatca accatatcca atattgcttc aaaaaaggag
    10501 caactggcgt gaagactaca agtaaaaaag aaagcagact gtaaatagaa agtccagcca
    10561 tcaaaaacaa gaaaactttc gataatgctt ggatcacgtt tctgctctct ttcttttgtc
    10621 catatagttc agagcgaagc cccacactaa caaacccgcc gaaaccggca atattgttga
    10681 tcgtattgat cagccagctt gtctcaaaca ggtaacgagg attctgcttt tgttcaagca
    10741 tccgattcaa aataatatca taaccaacca taggtaacac agagataaga ccaatcaata
    10801 gcattagtcc gcttttccat acaggtatag tggcaaacgt ttctcctaat tgctggatag
    10861 atagagtttt accgatggac atcaattcac cgacaataat gacaatgaca gaaatcaaaa
    10921 agattgtttt gaacagtcct aggtggtttt tcagccattg tatggtattt tttaacaaat
    10981 aaatcacctt ctattcctaa tagttatgtt tcagaaagaa attgaagaac ttctcgattg
    11041 aagcgttcag gatcttttct agctaattca tgtccttgtt ctttcacaag cacaaaggaa
    11101 gcttttggta tagttttagc tatatacaaa gaatgtttta gcttgattac atcttttttt
    11161 cccacgatga tcaaagtggg ggagttgatt ttttttaaat cattttctgt aagcccgatg
    11221 tcatgcagca aaagtttgat aactaaaaga tttttgcgaa gacttgggcg aaataaagag
    11281 agtaaccata cccatgcata atgaatatta ctgatgactc tggcagaaaa acgaacgcct
    11341 ttaacaagcg tgtttccaga atttaaaatc aatcgatgaa cttttttagg gaagctgctg
    11401 gcaaatacca gtgctagatt tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt
    11461 ttttccagca acatgatcgt attcagatcc tcggccatta actgaaaatt caacatagat
    11521 gcttcgtttg tcgaacgacc atgtcctcgg ctatctacta gatacacggt atagtagcgt
    11581 tcgagtacag ggacctgttc tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa
    11641 aagagaggga agccttgacc gctcttttca tagtaaattt cgctcccatc tggcattttt
    11701 gcatatttct tttctttttt cacataatca cctactgtgt aaagtataac gattatatca
    11761 gtcattgggc aattgttttt actgaaaaac aacgatcatt ttcgtaagat gtcaaaaaat
    11821 acaggaaaat tcattttttc ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa
    11881 gataaagaaa tagtaagata aaaaaagatg tcaaaataac taggtgatga aatgaagcga
    11941 aaacgattat ctattatccg tattcttttt tacatgatca ttctgacggt tattggtgcg
    12001 gtgttgtttt tatgaggaag aacttatcgt tcggtaaaac gagtggaaag ctatcaaata
    12061 gaaataacaa cagctgcaaa aaattatcat atggaaaact acgagaattt gatcaaggcg
    12121 ataatattga ccgaatcaaa aggaaaaggg acagatctca tgcagagctc ggaaagtctg
    12181 catggagaag ctgagatcat tgataatcca gctgacagta ttgatcaagg tgtcaaatat
    12241 ttggcggaaa tgatagtaga agcaaagaaa caaggatgcg atttatggac agctgtccaa
    12301 gcatataatt ttgggaaaga ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca
    12361 gtagaattag cagaagcgta ttctaaagat gtgctgagcc cacttttagg aaatgaggaa
    12421 gggaaacaat accgatattg gcggatccag tccttgcttt acaatggcgg ctatctctat
    12481 cataatggag gtaacatgtt ttatcctgat attgtcaaaa tgaatgaaaa aataatagag
    12541 acctatgaaa aagtctttaa gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa
    12601 tgaaagaagg agtgcgcaaa tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg
    12661 gtacacaacc aaagactgga tgttacggtt tgtgaaggga atgtttatcg gttcaggatt
    12721 tatcttgcca ggagtcagcg gtggtgcatt agcagctgtc tttggcatgt atgagcggct
    12781 gatttctttt ttagcccacc ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc
    12841 tgttggtcta ggcggagtga caggcatctt tctactttct tttttagtca gttttttatt
    12901 aggtgcgtat gaaacaacga tattatggtt ttttgtcgga tgtatcgctg gaactgttcc
    12961 tgcgttatgg aaagaagcag ggaaaaaagg aagagatact tctgatgtga tcatcatggt
    13021 tgttactttt gttctggctt atttgttttt attatacggc gctcaattat ttgacgggca
    13081 agtcgatcag aattttggaa catggctgat tgccggcgga ttgattggtt tgggtatgat
    13141 cgtcccagga ttgagccctt ctaatttttt ggtgtatatg ggattgtata aagcgatggc
    13201 tgacggattt aaagaagtac gacttgaagt gattgtgccg atcggaattg gtgggattct
    13261 ttgtgttctt ggattgtcaa aaatcatgga ttacattttc tcaaaggcat atagcaagct
    13321 attccatttc attttaggtg tagtttttgc ttcaacgatt atgatcattc ctaccgatta
    13381 cgcgggattg ggagttgctg gggtactagc ttgtcctgtt ctttttatag ccggtacagc
    13441 actaggttgg tggatgagcc gtttggagga acaatataag taaagataga ttgataataa
    13501 aaacagcaca cgggtgattc aaacaaaaag gttggcatct tttttgtaaa atcattcgtg
    13561 tgtttttcta taaattttat aaaaacaagt tgtttttaat cttggaacac gctagactta
    13621 aacacgtgat tatttttgaa agttggcgga aaatggagaa aaagaaaatg aatggactta
    13681 cacagcaaga ggtagttcag aaaatggctt ctggccagca aaatgactac caggaagatg
    13741 catcaaaaag tacaaaacaa atattcagcg ataatatatt gacgttattc aattttctga
    13801 actttggtat tgggatttgt ctgttatttg taggtgcgta ttccaacatg gcgtatttag
    13861 caattattgt cgtcaatatc gttattggaa tttatcaaga aatacatgca agaaatcttg
    13921 tacggaaatt atcgattgtt tcccaatcag aagtactagt gatccgggac ggtaaagaac
    13981 aatcgatttc tactaaagag ctggtattag gagatttggt aaaaatcgga gcaggagaac
    14041 agataccagc cgatttacag gttatatctg gacgggcaga agcaaatgaa gctcttttga
    14101 caggcgaatc tgatttgatt gtcaaagaaa ctggagctga attgctttct ggaagctttt
    14161 tgacaagcgg ttctgtcatg gctgaagtta tccgagtagg tgcagataat tatgctgtcc
    14221 gcttgactag cgaggcaaag actcacaaag ctattcgttc tgaacttgta gattcgattc
    14281 gcaaagtatc caaattcact agtttcgtga ttattccttt ggggatcata ctatttttgg
    14341 aagcactgtt catccgcgat gcttctatca aaatttctgt tattgcatct gcagcagctt
    14401 tgttaggcat gcttccaaag ggtttggtat tgctaataag tattgcatta acgacagggg
    14461 taaccaaact ggcaaaaaaa cgaatccttg ttcaagacat gtattcaatt gaaacgctgg
    14521 cccatgtcga tacattatgc ttggataaaa cagggaccat tactgaaggg aaaatgagta
    14581 tccagcgggt agaagcacta catccgctgt acgagaaagc catcccagaa attatgggga
    14641 cgtatctggc agaaagcagt gataacaaca tcactatgcg ggctatgaga gattattttg
    14701 agctttctaa tcggtatgat gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg
    14761 gcgcgatgga atttccagaa ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg
    14821 aagaagatcg gcttccggac agtctaaaaa cagcccaagc gaatggctat cgcgtactta
    14881 tgttggggat cgctccaaat cagcaattga acgaagaaaa accagcattt gttgagccgg
    14941 ttgctctgtt tgaaatcgat gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc
    15001 ttcatgagga aggtgtggat ctgaaagtga tttctggaga caatcctttt actgtctcca
    15061 atatcgctag acgtgcagga ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta
    15121 ctgaagaaat ggaagtgcga gaagctgctc atcgctatac ggtttttgga cgagtgagtc
    15181 cgcagcaaaa gaaattgctg gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga
    15241 caggcgacgg tgtgaatgat gttttggcgt taagagaagc agattgcagc attgctatgg
    15301 cagaaggtga tggagcaaca cgtcagattg cgaatctggt attgttggac tctgatttta
    15361 ctacattgcc agaagtcctg tttgaaggac gccgagtggt caacaatgtg acgaaagtct
    15421 caggcatctt ttttatcaaa acgatttatt catttatttt atcgattata tgtgccgtta
    15481 cagcaattgg atttccgttt atccctatcc aaatcacgct gattgattta gcgattgaag
    15541 ggtatccttc cttcttttta tcttttgaac aagataaaag aaaaatcact taccgttatt
    15601 tgcctacagc acttattaat gctttaccaa atgccttgct tgtagtagtg aatatcattg
    15661 cggtgtatct acttggaaaa acattcgcat ggggaaatgc tgaaactact actttgatgt
    15721 attacttgct gattgggatc agttgcatgg cagttgtaaa agcttgttat cccttcaacc
    15781 cattgcgcat cttcctagca gtaacgacga tcgtagggat atatgtagct gcaatgctgt
    15841 tccatggaat tttggaagta aacttagggt tgggagagag cgtaggatac ttctttgtat
    15901 tcatgctcgt aaatattgtt cttcgagtat tgtactggaa ggctgatatt caaaatcagt
    15961 tactgaaaag agtagcaaat tgatgcttaa tgagatagaa atgtgttata cttgaaaaag
    16021 caatgaaata tgatacaatc aaacatgtat aattgtgtcc tttaaaatcg cactgatttc
    16081 agttagtgcg attttttacc ggttattctc ctgaaatatc ttctgttaaa cccaaaagat
    16141 atcaatagag cagggggtga aaaaatggct caacggattc ctcaagaggt tatcgaaaca
    16201 gtacgcagtc aaacgaatat cgttgaggtg attggacaat atgtccagtt gaaaaagtct
    16261 ggtaaaaatt acttggggct gtgtcctttt catgaagaac gtacaccgtc tttttctgtt
    16321 gcagaagaca agcagatttt tcattgcttt ggttgtggta aaggcggaaa cgtgtttact
    16381 tttcttcaag aactagaagg tctaagtttt ccagaagcag tggtaaaagt ggcagacttc
    16441 gaacagatac cgttagaaga tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa
    16501 tctgctacgg ggaaactgat tgctgctcat gaaaaagcag cggaaatcta tcatcacatg
    16561 ctattgcata caaaagctgg acagccagct ttagaatatt tgcaaagtcg tgggttgtct
    16621 ttagaattaa ttgaagaatt caacataggt tttgcaccta atgagcgttc ttttcttcag
    16681 caagtattca agaatgaatc aatttcaaat gaattgttag aacgctctgg attgtttgtc
    16741 acgcatgatg atggccgtat gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt
    16801 aattctcaag gccagacgat cggtttttca ggacgctggc ttgcacctga aaaagggcag
    16861 gaaaaagatc agcccaaata cttgaatagt ccagaaacag aactgttcaa taaaagacaa
    16921 gttttattta atcttgataa agcaagaagc gatatccgga aaaacggaga aattttgttg
    16981 ttcgaagggt ttatggatgt gattgctgca tggcaggctg gtatcaaaac tggattagct
    17041 tctatgggga caagtctaac agctcaacag atccaacaaa tggaaaagct gacgaaagaa
    17101 ttggtctttt gctatgacgg agacaacgca ggttttgaag caaccaatcg agggattgaa
    17161 ttgcttcggc aaaacagtcg attagcactt tctgtcgtag tcgttccaga aaagcttgat
    17221 cctgatgaat atctccgtaa gtatggagaa gcatctttcc aagaattagt ggctcatggg
    17281 cgcgaaacag tctttacttt taaatcacgt tatttaaaac aagggaaaaa tttagataat
    17341 gaaaaagaca agatcgaata cctcgaagaa ttactagttg aacttagccg agttgtttca
    17401 ccaatcgaac aagatatgta tctatctcaa ctgtcaacag agtttcagat cggcagagag
    17461 acgatacaaa aacaactgcg cgatcttcga aggacaaatc agcaaaatcg tcaagaacct
    17521 caggaaagtc aacacactca ggttacgaga caagaaactg cacggaaaaa gagacctttg
    17581 acgcaagtag aaaaggcaga gcagattcta ttatatcgtg catttaaaga agtttctgtc
    17641 cgtaatattt taaaagaacg agaatttcag tttattcatg atgcatatgc agaagtttat
    17701 tttttattcg atgcatatgt ctcacaacat tatgaattca accttgcaga gtttttagac
    17761 tttttgcaag atgaaaattt aaggcggttg gtggtggaaa tcgaatattt accggtggca
    17821 gaagaaagtt cgccacgaga aatccaagac ttgctagatg tgatcagcaa atctggctta
    17881 gctgatgaaa taacattaaa aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa
    17941 cagcttgagc ttgaattgac gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc
    18001 aaatagctac tcaagggggg ccttctttta tggcaaatgg aacagataaa aaaacgtatg
    18061 aagcagcagt agcagctttc atcaaagaaa acaaacccaa acgacaagtt gtatatgatg
    18121 atttatccaa tcagcttgct acaccattca ctttgaatgc agatgaaatg gacaaattga
    18181 tccaaaagat tgaagacgca ggtatcagtg tggtggatga aaatggtgat ccatcaattc
    18241 acagcttgaa gagtgctgag aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc
    18301 caaccggtgt aaaaatcaat gacccggttc gtatgtatct gaaagaaatt ggccgtgtct
    18361 cccttttaac tgcggaagaa gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag
    18421 aagcgaaaca gcgtctggca gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt
    18481 atgtaggacg aggcatgcag ttccttgact tgatccaaga aggaaacatg ggactgatga
    18541 aggcagttga aaaatttgac taccgcaaag gatttaagtt ttcaacctat gctacatggt
    18601 ggattcgtca agccatcact cgagcaatcg ctgaccaagc acgtacgatc cgtattccag
    18661 ttcatatggt tgaaacaatt aataaattga tacgtatcca acgtcaattg ctgcaagacc
    18721 ttggaagaga acctacgcca gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag
    18781 tccgagaaat cttgaaaatc gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag
    18841 aagatgactc tcacttaggt gatttcatag aagaccagga agcaacaagt ccagcagaac
    18901 acgcagctta tgagttacta aaagaacaat tggaagatgt actagataca ttgactgatc
    18961 gggaagagaa tgtccttcgc ttacgttttg gtttagatga tggccggacg agaacattgg
    19021 aagaagtagg gaaagtattt ggtgttactc gggaacgtat tcgtcagatc gaggctaaag
    19081 cattaagaaa attgcgccat ccttctcgtt caaaacaatt aaaagatttc ttagaataag
    19141 cgtggactat tgaccagaag agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa
    19201 aa
//
LOCUS       DANKAZ010000048        18743 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000048 DANKAZ010000000
VERSION     DANKAZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            111..1538
                     /locus_tag="PWY67_001632"
     CDS             111..1538
                     /locus_tag="PWY67_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2057905.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1630..1995)
                     /locus_tag="PWY67_001633"
     CDS             complement(1630..1995)
                     /locus_tag="PWY67_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2057906.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1992..2357)
                     /locus_tag="PWY67_001634"
     CDS             complement(1992..2357)
                     /locus_tag="PWY67_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2057907.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2549..2797)
                     /locus_tag="PWY67_001635"
     CDS             complement(2549..2797)
                     /locus_tag="PWY67_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057908.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2796..2924
                     /locus_tag="PWY67_001636"
     CDS             2796..2924
                     /locus_tag="PWY67_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL2057909.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2981..4675
                     /locus_tag="PWY67_001637"
     CDS             2981..4675
                     /locus_tag="PWY67_001637"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL2057910.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4679..4798
                     /locus_tag="PWY67_001638"
                     /pseudo
     CDS             4679..4798
                     /locus_tag="PWY67_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4775..4948
                     /locus_tag="PWY67_001639"
     CDS             4775..4948
                     /locus_tag="PWY67_001639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057911.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5326..6459
                     /locus_tag="PWY67_001640"
     CDS             5326..6459
                     /locus_tag="PWY67_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL2057912.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6490..6810
                     /locus_tag="PWY67_001641"
     CDS             6490..6810
                     /locus_tag="PWY67_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL2057913.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6839..7303)
                     /locus_tag="PWY67_001642"
     CDS             complement(6839..7303)
                     /locus_tag="PWY67_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2057914.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7435..8688)
                     /locus_tag="PWY67_001643"
     CDS             complement(7435..8688)
                     /locus_tag="PWY67_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2057915.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8717..9034)
                     /locus_tag="PWY67_001644"
     CDS             complement(8717..9034)
                     /locus_tag="PWY67_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2057916.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9060..9380)
                     /locus_tag="PWY67_001645"
     CDS             complement(9060..9380)
                     /locus_tag="PWY67_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2057917.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9844..11259)
                     /locus_tag="PWY67_001646"
     CDS             complement(9844..11259)
                     /locus_tag="PWY67_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2057918.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11276..12592)
                     /locus_tag="PWY67_001647"
     CDS             complement(11276..12592)
                     /locus_tag="PWY67_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2057919.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12742..13440)
                     /locus_tag="PWY67_001648"
     CDS             complement(12742..13440)
                     /locus_tag="PWY67_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL2057920.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13729..14685)
                     /locus_tag="PWY67_001649"
     CDS             complement(13729..14685)
                     /locus_tag="PWY67_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2057921.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14798..15631)
                     /gene="msrA"
                     /locus_tag="PWY67_001650"
     CDS             complement(14798..15631)
                     /gene="msrA"
                     /locus_tag="PWY67_001650"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2057922.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15806..17440)
                     /locus_tag="PWY67_001651"
     CDS             complement(15806..17440)
                     /locus_tag="PWY67_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057923.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17427..>18743)
                     /locus_tag="PWY67_001652"
     CDS             complement(17427..>18743)
                     /locus_tag="PWY67_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057924.1"
                     /translation="FSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNR
                     EKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAV
                     FLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLL
                     GLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILG
                     IQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIV
                     INEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYT
                     VASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPT
                     ALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT         5917 a         3695 c         3140 g         5991 t
ORIGIN      
        1 ttttaacggc atttatatgc tttttcagaa attaactgat gatttcaaaa agaaaacatg
       61 tcataatatt tcccatattg tgcaaaaaaa tacaaggcgg aggctctact atgattgaag
      121 aatatattga gaaaaatatt ttaagacagc tgtttttatg tgggcaattc tatgtaaata
      181 aagaagtcaa tctagaaaag ctctccaatc tcctgcatgt ttgcaaaacc actttactca
      241 atgatatcaa taacataaaa aaagaatttg aagaacagat cgcttataca catcgggaaa
      301 aggaccgtta cactttatat ttttcagaac atatacctcg atgcaagatc atgcagcaat
      361 tgtctcaaaa ttcgttgttt ttaaaaactt gtctgcttta tttggaagaa gatgaacctg
      421 attatcttca attaactgaa tgtgaattca tctctgtcag caaagcttat tcattaaaaa
      481 aacaagtatt agcttacttt aatgactgcg ggatcgaaat cgaccgctac tcccctcgat
      541 tcactgaaat ggaaagacgt ctgctgcttc ttaatgtgtc gtatcgttta ggcggtttta
      601 attcttggga gctacctgaa tcgttttttg aacgtgccga tcgtttcatt gaatccgtaa
      661 cggaaaatag tggcagattt tatgataaag aaaataaaga aatcttatcg attggttttg
      721 ctatcagttt cctacgccag caagtgtgtg ctgtgacaat agattcaaaa tttatcgaag
      781 aaatcaaaaa acgtcctatt tacaattatg tcgaatcagc atgggaaaac actgatttcc
      841 aaacctatta taaaaaagaa gaattcgcct ttattttgac actattcaat ctttgtaatt
      901 atggtttcca ttcttatcag ttgattgcgg aagactttca acagctacac caagtgttta
      961 ttgacaatac tcccgaaata aaagaactag ttgctacctt tgaatctcac ttcaatcaag
     1021 aattattcgg aaaccaaccg ttcgaacgtg cgctgattca tctgatgcgt tcggcttggg
     1081 ataactatca attattcatg ccagaaaaat tttatctttt aaatgaggaa cagacaaatt
     1141 tatataaaga agtccagaca attttttcca gctggtccag ccagttacct tatgatctac
     1201 ggttgaatcc taattgcatg cgggcttttg tgatcgaatt gagcggaatt ctccgtctga
     1261 caaaagaaca tctgactatc tatatcgtca caaattcgga tgttcattat ctgatttatc
     1321 gggaagcctt ggaagctgta actactttcg attttcaagt tgcaccaaca atttattcaa
     1381 gtatatctga tataaagaag tatgcgcagc agtcctcaaa ccgggtatta tgtgaacgta
     1441 cattatatac accagatgcg gtccaatatg aaaatattat tcctatttcg atcaatacga
     1501 tcgatcgagc tatcatttca gctgtgcaaa acaaataagg ggctgtgaca aaaatcatgt
     1561 cacagcctct tatttgttga agatgcccat ttctgtaaca atctcttgat acacgtcagg
     1621 ctcacaacct cacttatttt ggacatctag gggtccgatc tctttattta aaaaataaga
     1681 aatgatggtt cggataacta ccaacgccgc tagttggaaa atatcctgca aggtaggttt
     1741 gataatcgat tcgatgatcc cagctgaaac aagaatttcc aaacttagaa gtatatagcc
     1801 tcccaacatc tttttaatag cattgttctc gcttactcgg tcgctttgca cttttgtatg
     1861 gacaaataaa tatttcaaaa aatccttgat tgatagacaa acaccccaaa ccaataaaca
     1921 gatagcaaag atatcaagtc ccagtacaag tttttcgaaa tatggaagaa gaaactgcat
     1981 caactctgtc gtcatcctac ctctcctctt ctttgttttt ctttgtcttc tatttcctga
     2041 ttcaagaaat aagaaatgac tgttcgaatg ataataataa gtgcaagctt caaaatatct
     2101 gtccaagtcg gcttgataac tgactcgatg atatcagcaa cgactagtac ttccaagctt
     2161 aacaaaatat aacttgctag cagttttctt gtatctgcat tacgagtatt acgttctgca
     2221 tagtttttct tgacatgaga ccaatcaaaa agtttgaata gaacaagaac gatcccgaca
     2281 attaagatac cgatagaaca aatatttaaa atagcaatga tccattctag aaattcaatt
     2341 aaatttgagc gaaacatata cgctacctac tttcttttaa tttgttccta ttcttttatt
     2401 gtaaaagaaa gttaccttgt tcgtctattt aaaagttgtt ctaatgataa cggttcgctt
     2461 aaatttttct ttttcactct cccatttttc tccctccatc cataaatcgt ggaagtatcc
     2521 aaaacaacgt agcaagttaa aataaggctt agtccgtcat cagcttttca aagaagcact
     2581 gtctcggtgc tttttttgta cccaaatttc taaaaataga accatacacc tcctcaaatc
     2641 ttttcttctt acctacccta tttttattta caggaaaatc agacacttct tcttttttgt
     2701 caaaatttcc tttttcttct cgtcctatca tttggcattc taataaaaac gaactatcct
     2761 tggatataga aagagaaaac aaggaggcgt ttttcatgag taaaaaatta atcaaagtcg
     2821 gcatcggttt aggtttgctt gcccttggcg ctgcatatct aggaaaaaag acaggactat
     2881 tcgaagatga cagtcattta tatgatgaat tcgaatcaat ctagcatcct atttagttga
     2941 atcactacgg acatacttgg aagacattgg aggaatcaca atgagaaaaa gtaaagcaat
     3001 aatgattggt gccggtattg ccaatatggc agcggctgtt tatttgattc aagaaggcaa
     3061 atggaaaggg gatcagatca ccttttattc attggatgat cacggttcaa atgatggtgc
     3121 accgaccgac actgtagtgg atgaatattg gaataaaaac catccattag aaaaccaaaa
     3181 aggctatatt gctcgaggcg gtcgcatgct taattaccgt acctatgtag atttgatgga
     3241 tctgttaagt cgaatacctt ctgttactga gccagggatg actgctgaag aagatacgag
     3301 agattttgat gcaaaacacc gaacctttga caaagcacgt ttgatggaag gtggcaaagg
     3361 aatcattgat ggcggaaaac tgggattcaa taataaagac cgtattcttc tgactaaact
     3421 gattgctatg cctgattcag aagaagaaaa actagacaat atcactatcg aagaatattt
     3481 caaagatgat ccacactttt ttgaaacaaa cttctggttt atgtgggaaa caacatttgc
     3541 tttcagaaca cgtagttctg ctcaagaatt gcgtcgctat atgcatcaaa tgatttatga
     3601 attcactcaa atcgaacatc tagtgggcgt caatcgaaca cgttacaatc aatacgaaag
     3661 tattatgctt cctttgatcg agtacttgaa agaacaagga tgcaaaatca ttttgaaccg
     3721 ccgtgtcact gactgggaat tcaaagaaac accgatgcaa gatgaaatca ctgtgactgg
     3781 tttgaaaatt accaatacac tgactcaaga agaagaatac gttgtggtcg atgaagatac
     3841 agcagttatt ttcactaatg ggtccattac agattcagct actttaggag attacgaaac
     3901 accagctgta gaaaatatgg attatggtgc agcttctagt ctctggaaaa aagccagtga
     3961 acgtttctac aacttgggaa atcctgacaa attctttgct gatcgagatg caagcgaatg
     4021 ggtcagcttt accctgacga cgaaaaacca tttgttgttg aatgaaatca cccgtatcac
     4081 gactcaagaa ccaggaaatg ccttgaattc atttatttca accacaccga tcacgccgtt
     4141 aggccaaaaa gatgtcaata tgtctattgt cgtgcatcat caaccgcatt ttacgtctca
     4201 gaaaccaaat gaaacagtta tttggggtta tttcctttat ccaagacgac gtggcgaatt
     4261 tgtggataaa cagtatatta aaatgaatgg aaaagaaatg ctagaagaac tgattggaca
     4321 actttcaaaa gtagatcctg gaccagtgaa tattcgtgaa aaagaaacag aaattttcga
     4381 cagcgtaata aataatatcc ctgtctatat gccttatgct tccgcgttgt tcaataatcg
     4441 tgccaaaagc gatcgaccaa aagtcattcc taaacattcg accaatctgg cctttacagg
     4501 agaatttgtc gagcagcctt atcagatgat ctttactgaa caaagtgctg tacgttctgg
     4561 agaaatcgct gctttccatt ttgcgggtat tcctatgtcc cgtcttgtca aaacaccgcg
     4621 ttacgacaaa gatattccta ctctagctcg tgccgcaaaa aaaatgtttg aataatcaag
     4681 aggttgtgaa aaagctgccc tgcatcaagt aataagaaca accaggctaa aacagcttct
     4741 tatgtttttg atttggttgg acttattacc gcagatgcaa gcttttgaac accgtttacc
     4801 aagaggttat gaaaaagctg ccctgcatca agtaataaga gcagccaagc taaaacagct
     4861 tcttatgttt ttgacttggt tagacttatt accgcagatg caagcttttg aacaccgttt
     4921 accaagaggt tatgaaaaag gcgtttagtt tcgagtatga agaaaaattt tgtgaaaatg
     4981 gttcctcacg tttttgcata aatttgactt aataccgaga aactgatccc ttctgtcata
     5041 acctcttttt atttatgtct gtttcttttt gaaaacaact attttttcaa cgtttactct
     5101 cttgccaatc caacatcatc taacggatac aactatttca atgattacca ttacactaaa
     5161 aaatcggaaa cacacctctc cgaacacaaa tacataagac tatagaatgt ttcatttaag
     5221 aaaaaaagcg tttcattatc aaaaaggtaa gatctccttt aaaatgaaat tatctgtagt
     5281 actaataatg cagacaattt tatgaatctg aaaggagcat ctccaatgaa agcagtcact
     5341 tggcaaggaa aacagaaaat ggaaatcaga aacgtagatg atccaaaaat attagaacca
     5401 acagatgcaa tcatccgtat cacagcaaca gccatttgcg gctccgatct gcatctttac
     5461 catcatggtg aagcagtttt ggaaaaaggg tatgttgtag gacacgaacc aatgggaatc
     5521 gttgaagaag tgggatctca agttcaaaca ttgaaaaaag ggcaacgtgt ggttatccct
     5581 tttaatatta gttgtggtca ttgtcatttc tgcctgaatc acatggaaag ccaatgtgat
     5641 gaatcaaatc cagatcagtt atatggcggg cttttcggat ttggcaagtt gaacggtaat
     5701 cattggggtg gtcaagcgga atatctaaga gttccttatg cagactctac ttcatttgtt
     5761 gtaccagata atgatcttcc tgatgaaaaa gttttatttc tatcagatat tttgcccaca
     5821 gcttattgga gcgttgaaaa cgcaggagta aaaaaaggcg atacagttgt gattctaggt
     5881 tctggtccag tcggtctatt cgctcaaaaa ttcgctgtta tggctggagc agagcgcgta
     5941 atcgcagtag atccagtaca gcaccgtcta gataaagcgg ctaattacaa tggggttgaa
     6001 acttatttct tagaagacac ggcacaagct ggaacagaat tgtatgaact ggctaaaggt
     6061 ggcgcagatg tcattatcga ttgtgtagga atggacggat tagagcctgt caaagaaaaa
     6121 gcgaaaaatt tagtaagctt acaaagcggc acaatctctc cattacagat ggcatcgcaa
     6181 gctgtcaaaa aatttggaac agtccaagta actggtgttt atatgactcc agcttcttct
     6241 tacccgctgc aagaattttt tatgcgggat atcgctgtca agcatggaca ggcaccaatt
     6301 atccatctaa tgccaaaaat ttacgcaatg attgcagaag gactattcga tcctacacaa
     6361 atcattactc attcgctgcc actatcagaa gcagcacaag catatgagat ctttgacaaa
     6421 aaagaagata aaaatataaa agttgttttg aaaccttgaa ttcagtaaaa aatacaggtg
     6481 attttcttta tgcccaaaat caaacgaaat acccatgaga tcgaaatagg aaaagaagaa
     6541 gatatcgaaa tacgaggacg tcgcttccgc ttctatttcc aaaatcgtta cacattgatc
     6601 tcactggctg tcgatttatt aaccgggatt ttctatattt ttggaagcct tgcttcctta
     6661 actaaaatcc ccgatcgata tggcacctat ttttatttgg ctggtgctat tttcttgacg
     6721 attcgtccta ttctcagaat cgtaaggaac atttttattt acgacgaaaa aaaagcaatg
     6781 aagagaacaa gcaaaaaaaa agaggcatga gaatttttca aatctcatgc ctcttttttc
     6841 accctatcgt atcactttga tacagacgag cggtcgtccc actttcttcc ttgaccattg
     6901 tcaaaaatgt ccactcgtat cgttcataaa acgtggtatg atctgtaatt aagtaaagtt
     6961 tttcgtaccc aagttgatgc atttcttttc tgatttccgc taatatccgc ttggctatcc
     7021 cctgcttacg cttttccggt tcaacaaaca gggcacaaag atttggtgtc agatcagggc
     7081 ggttatggaa gtcgttttca atgacgcccg ctcctccgac gatggtttct ttttcatcta
     7141 agaccacata ccattgagga atactttcct tattagcgat tccagtacga atactttcct
     7201 catacacaac agcaggaact ccccattttg atgaaaacca attcgcgctt tgttttacta
     7261 attctgggtg ttctcttaat ttgatccagt tatatgtaac caatgtttaa acctccgact
     7321 aaaataaaac gtttacaaaa attatacaag tctttatgaa aattagcaat gacaacaata
     7381 gtgatgtaaa aaaaaaaacg gaaaacaatc acttgttctc cgttttttga ttaatcaagc
     7441 atctaaagct tcttttcttc tttcttggat cgtagctacc ttgacgaacg gtagatagat
     7501 caaaatagaa agaaccaaat tgacagcggc tagtacacca cctgcaatac tttttgttgc
     7561 caatacaccg ccaataattg gtggagttac ccatggaggc ataacggtag ctgctggcac
     7621 taaacctgta gaagtcgcaa agtaagcaac actaactaag accattggtg tcaaaataaa
     7681 tggaatgaac atgatcgggt tcaacacgat aggtaaccca aacatcgttg gttcattaat
     7741 attgaagaca cctggcgcaa tcgacaagtt tgttacaacc atatatggtt tgtttttacg
     7801 tcctactaaa taaatagcta acagtaaacc taaagttgca cctgttcctc ctaagttgac
     7861 aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg ctatgtccag ctgcgattgc
     7921 attaacgttt gcttcaatgg ctggcgcatt gattgtttgc atcaaaggat cgaccatatt
     7981 cgctccgtgt aaaccaaaga accataagaa tggtgtgata aatgcaagca gtaaggcaga
     8041 tggataagag tttgcaagtc ccataaatgg ttcttggacc actgtataga aagaacctac
     8101 gatatcatca acaccgaatg cagcaaaaat tgcagcaact aaagcaaacg cagagatagt
     8161 gatcattgct ggtagtaatg aagcaaatgc ttttgcaact gccggtggca caccatcagg
     8221 cattttgatg atcaattttt catttccgct taaacgtaca aagatttctg ttgaaataat
     8281 cccaacaata atagcaataa acaaaccgtt tgagctcata cctaatgctc cgcctaaagc
     8341 gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg tcttttcggt agccgttagc
     8401 taaatgataa gcaagcaaga aagcaatcaa gacagaaaga atggcaaatg taccgttcca
     8461 agcagcagcc ccgaatcctt tccatgattc accaccaaaa attgagttca tcaattcttg
     8521 gaacccggga atcggtaagt tattgatcat tactgctaat gctcctaaga tcattaacgg
     8581 catactcacc ataaatgagt cccgaatcgc aactaaatgc cgttgtgcac caatttttga
     8641 tgctacaggt ataaaatgtt tctccataaa tgcaacaaat ttatccataa atcttccctc
     8701 ttttctttac tgtagattac gcgttcaatc gacgatacag attaatgatc tcttttgcta
     8761 aatctgtaaa agctatagat gtcatcagat gatcttgact gtggaccgtc aaaagcgtga
     8821 cttcaacgtg atttccttgt gcttcttgtg tcagcatctc tgtttgagag tgatgtgctt
     8881 ggactaaaga ttcctctgca tctttgattt tttcatctgc taaagtgaaa tcaccttttt
     8941 tcgcagcagc aatcgcttcc attgcatcgc tttttgcatt tccgccaaac atgatcagac
     9001 ccatgatggc ttctagattt ttttgatctt ccatttattg tatcctccga taatttttat
     9061 tatttgttta ttagtttttc tgcttggtcg agtacttttt caccattcat catgccatag
     9121 tcagccatat tgattacatc taaaggaatg cctttaggtg ctagcttttc agcaaattga
     9181 ttttgcataa agcgtacttg cggtcctaaa agtaaaacgt ctacattttt agattctagt
     9241 tgattatccg catctgaagc agatacagca aagatgtctg cttccattcc ttttgcttct
     9301 gctgcctttt gcatttttgt gactaggaga cttgtactca ttccagctga acatacaagc
     9361 atgattgttt tcttttccat tttaaacacg ttcctttcta tacttacact tgtatataag
     9421 caagtagcgt gccaacttta aaagcgcttt aaaaagttga tatcatcaca tttaaggtct
     9481 ataccaagtt gcatacacac taaaaaagaa agcgcacact aaacacacaa attaattttt
     9541 attacctttt ttcttttaac gtgcacaaaa aaagttgtga cacaaaactt gtcacaactt
     9601 ttttcaaata aacggtattc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa
     9661 aacataagat gttgttttta ctttgctatt cttattactt gatgcaggac agctttttca
     9721 caacctctca ataaacggtg ttcaatcagc tgaccctggg tattagctca aaacgctgga
     9781 ttcacaacct cttttatttc aaataaccag taaatacaat gaacatctac aaaaaacttc
     9841 tatttattta tagatttcgt caggcaaatc caattctctc ttatcaatca aagtctgata
     9901 ccacgaagca gattttttca atgttctgct tcgttcatcg ttcaattcga ttcgaactaa
     9961 tccatatcga ttcttaaatg cattcattgg tgacacacag tcagtgaatg cccataacat
    10021 atagccttcg caatttgcgc cttcttctgt tgctttgatc aagtaatgca aatgctgtcc
    10081 gataaattcg atacggtaat catcttgcac gattcctttc tcatccatat actgttcttc
    10141 atattctctt cccataccgt tttctgttat cagccaaggg atattatgat aattttcttt
    10201 tagatacatg gctatatcat acatgatttt cggatagatt tcccatcccc tggaattatt
    10261 cattttcttc ccaggtaaat caaagacttc ataatatcgt tcaggatgga aaggtgtctc
    10321 ttcattccat tgataacgag gggcgcgaac gcgtttagga taatattgat tgattccaag
    10381 gaaatctact gtatgatgct gaatcgtttc taaatcctct gttgcaggat cgaaataaat
    10441 cgattgtttt tgacacaact ccaggaactc ttctgtgtag tatcctttta ccatcggatc
    10501 aaaaaatact cgattataaa acaaatcata catataagca gctttttggt cttctggaga
    10561 attggatcga gcatagacca tttcaggatt taaaatacag ccaatccttc caccataatt
    10621 tccgctgcgg taaatctcta ctgctttagc cgttgctaaa actttatgat aattccacat
    10681 catccacttt tttgtgtttt gttcatgcgg ccagcgaaca gcatccaaat agcaacgcgt
    10741 ttggggaacg attggttcgt tgaatgtaaa ccagtttttt accctatcac cgtaacgatc
    10801 aaaggcaatt tgagcatact tagcataaaa atcaactact tttcttgaag accagccatc
    10861 atatttttcc aaaagaaaag ctgggacttc ataatgttcc aaacaaatca tcggttctac
    10921 gtctgcttcg atcaaagagt cgatcatttc acttatatgc tgagcatatt cttcatctac
    10981 tatacagttc tcataatcag taaaaaaacg agcccaattg atagaagtcc gataatgtgt
    11041 cagatggatg gatttcatca gatccacatc ctgctggtat tgttccataa aattagttgc
    11101 tacggctggg ccatacccat tatgccaaac aaaatgttca tttttatacc agctatctat
    11161 aaacgaatcc tgtccttctt tttttccttt ccaaccttcc gtttgccaag cagaagatgc
    11221 agcaccaaga ataaaatttt ttggaatagt aattttcatg atttcctcct gttttttaga
    11281 tcccatcatt ttccacacga tttgctactt ttacaaatgg cagatagatc aaaatagaaa
    11341 caataataca aacaagttgc gtaataactg caccgatatt tcctgctgtt gccagataag
    11401 cattgacgat cggcggcatt ggccaaggga ccattacaac tgctttcgcc gcaaatccta
    11461 aagcggttgc aaaataacca atcgttccag tgataagagg cgttaagata aacggaatag
    11521 ctaaaattgg attcagcatg atcggcatac caaaaataac tggttcattg atgttgaaga
    11581 tcccaggagc aagggataac ttggtgattt gtttcatatc ttcccgtttt ccaactaaca
    11641 atactgcaat cagtaaaccg atcgtgactc cagacccgcc catactcata tacatatccc
    11701 aaaatggcat agtaataatg ttcggtactt cttttccagc ttcaaaagct tccgtattta
    11761 cagcaattgc cgctaaaagc aacggttccc gtatcggctt caccatttga tttccatgga
    11821 taccaatcac ccaaaagact tgcgcaataa acatcaatag caaaataccc ggtaaccctt
    11881 gtacgacacc ttctaatggg cgttgtacga cattgtaaat gatatcataa aggtacattc
    11941 ctgtaagttc tttgatgata aatcccgcag ccgcgatacc cgatacagtc agaatcgtcg
    12001 ggaataaagc agaaaaacta gcagaaacat tcgttggcac ggtatcaggc attctcagct
    12061 gtagtttctt ctgtttcctc aaccagcaat aaagttcaat agaaaggatg gagaccaaca
    12121 ttcccaaaaa taaccctttc gtatctgtat attgtttagc caatacgttt tctactataa
    12181 cagtctgatt ttcattcact aattcataaa cagttggatt aacagataaa taagacatca
    12241 ctgcgatgat accagggaat acaccatcca ctttattcag cttggctaat tcgatcccta
    12301 tcagaaaaac agcatagatg gtcaagaaac ttagtgtgac atagctgatg gcagatgcga
    12361 taggctttaa ttcttctaaa aaacgcaaga aacttatctg tgctaagcca ttttccgagt
    12421 caaagaccat tgctgaaaac aatgtgccaa aagcacccgt aataattacc ggaatcaatg
    12481 cagcaaatgc gttttttatc accatgatat accttaaatt attgattctc gttgccacgt
    12541 tccctaaaac attggctaat ttatccataa aactcattcc agatcccctc attcctgctt
    12601 cttctccttg acgtcatctc tttttgtcta ttagtcagcg cttacatttc gatgaacaga
    12661 aactatttac tcctcttttt taccaaatat aaaacaaaaa ttcatctgat gtatttatca
    12721 taaaatacat cagatgaact gtcaattagt ttctttttct cctattattt tctgtaattc
    12781 tctccgattt attgccatat ggacagtcat tgcatcaaac gcagcaagtg cattccgcct
    12841 ttcgatggcc tgaacgatct ctgtatgaat atcgatcgtc tcgattttca gatgctggat
    12901 atcataattt ttattaaaca aagcaattga ttcattaatg attgggataa tatgcagcaa
    12961 ggctacgttt ccgctagctt ctgcaatcat cgtatgaaaa tcgatgtcta attgaacatg
    13021 ttcttgtgtg ccttctcgaa aagaagtttc aatcttttgc gttaattttt tcatttggac
    13081 gatttgattc ttcgttgcat attccgctgc caaacctgcc atcttaggct ccaataaata
    13141 tctcagttca aataagtctc gtatcatccg atcctgatct tcaatcaaag aaaaaccgag
    13201 tgggtcattg gcaatccctc gcttatcact gacaaatgtc cccgctcctt gccgaacttc
    13261 aaggatattc ctggaagcta gagcccgcac agcttctcgc accgtgcttc gccccacctc
    13321 taaataatcg gacaattcat attcattcgg cagtttatct cctacttgca actgcatttc
    13381 ctttaaatat ctaaggatct tgtcagtagt tacttctact agggttttat tttcttccaa
    13441 accacatacc tccttttcat ctgatgtatt ttttcgtaat tattataaca cgtttgaatc
    13501 atctgatgat tttttgtata caaaattgat taatccgttc atagacatac aaacgaagaa
    13561 gatatttacc ataaaaaaga ctaggataaa attcactcct agccttcgat catttttatt
    13621 ttgaacgaca aaaaatatga tgttcaataa cagacgatct tcattcgatt cttgatgtca
    13681 tcttttgttt tttttaatat atggataaaa agtttgatta catcgtttct attctagaat
    13741 ttggtcgatt ctcaattctt cttctggtga gaaatctaaa ttatccatca ttcgaacact
    13801 ttcttgtaac tgacttaacc gacttgctcc gaccagtaca cttgtaacag atttttgtcg
    13861 caaattccat gcaagggcca tttgcgctaa tgattgtcct cgtgatacgg cgattgtttg
    13921 cagtgctttg attttttcca gagttgatcc aacttgttct tctgaaagga atggtatttc
    13981 cttacgatgt gctctagaat cttcaggaat accatgtaga taacgatttg tcagcaaccc
    14041 ttgagcaagc ggactgaacg tgattgcccc tagcccttct tcttctaata ctggagatag
    14101 atcgtcttct agtagtgcac gagaaaacat attgtagcgc atctgatgaa taatgaacgg
    14161 tgcttccttt ctttttaaaa tttcagtcat tttttttgta tcttctccat tatagttgga
    14221 tataccaata tataatgctt ttccttgtct tacaagttgc atcaaagctt ctgctgtttc
    14281 ttccaaaggt gtatcagggt ctgggcgatg atgatagaag atgtcaacat aatctagccc
    14341 cattctttgg agactttggt cacaacttga aatgatggat ttacgagagc cccattcccc
    14401 atatggccct tcccacatat gataacctgc tttgctagaa ataatcagtt catcacggta
    14461 ggcttgaaaa tcttctctaa aaatacgccc aaagtttttc tctgcagctc cagcagggcc
    14521 tccataatta ttcgcaaggt cgaaatgggt aattcccatg tcgaatgcgc ctcttaaaat
    14581 ctctctttga ttatggatag gatcataatc accaaaattt tgccataatc cgagagataa
    14641 aatcggtaat ttcaaacctg aatgaccgga tttacgatat tccatttttt cataacgtgc
    14701 actatctgca atatacaatt tttctcctcc tcatcaaatc tttttaaaac gctttctttt
    14761 ttctattttt agtttactac attttaaaga aaaggagtta atcaaaatga ttttttcgta
    14821 tatttttttg ccatttgttt tttactcgtt gatagatcag aaattgatta cgtgcttgct
    14881 ggattttttt ataacgtttc ggttgttttt tgtaaaattg ctgatgatag ttttctgctg
    14941 gccaaaacgt accggctggt tcgatttttg tgacgattgg ttggtggaaa gttcctgaac
    15001 tgtctagttt ctgttttgat tgttccgcta attctttttg tcgttcgact tgataaaaaa
    15061 tcactggacg atattgcttc cctctatctt gaaattgtcc aaatgtatct gtgggatcag
    15121 taatttgcca atagattgtc agcaactcgc tataacttat ttctcttgtg tcataaatga
    15181 tctccacagc ttccacatga cctgtgtaac caccacttac ttgatcataa tttggttttt
    15241 caacatgtcc gcccgtgtat cctgacaaaa cagataggat cccttttttc gattcaaacg
    15301 gctcgaccat acaccagaaa catccccctg caaatactgc acgttcttga tagtcagcat
    15361 cttgcatggc atgcttggaa aagtcatatt tttgctcctt atcaaatcct tccgtaatca
    15421 ataaataaaa gtccattaca tcaggcgtca gattcattcg gatagctaat ggacgtagtg
    15481 ttgcttctag ttttgataac tgttctttaa cggatatatt ttctcttatg tcattctttg
    15541 tctgaatcaa tacttttcgt tcccaatctc gtgtattcgg attcaaaatc agattataaa
    15601 gtgtttgaat gaatatttgt tcctcatcca tagattctcc tcctttccga agaagacaag
    15661 ctagtgactt tagctgtaat ttatcataaa gtgaggaaat aagcgattac ttcatccccc
    15721 tacagatttt tcgtataaaa aagagagaat ccttatcgac agattctcct ttttaatcaa
    15781 tttattttat aaatgaaaag ttaatttacc ttttatggaa aactatttta aaaaataaac
    15841 ataaatatgc agttaatgta acaagataag aaataatagt cgttactgta taacttgtgc
    15901 ttactttaat atcagcaact cctttgactc cgcttatttg taccaatcca ttagcattca
    15961 cctgctcctt tacaggttga tgatttattt ctacattcgt tcctaaataa gcatatacag
    16021 gaagtgtgat tacttggctc ttctcattag ttactctata tatgatagaa ctaccagttg
    16081 ttttataagg aaggtttacc cattgactgc caatctttgc ttcgtgtttt attatatgct
    16141 ccttattctc caatgaagcg ataggagaat aatcatataa aaaataatcc attcctttag
    16201 tggtatacgc ctctactgtt tctttgttta caacaatatg tcccggctga gaaattattt
    16261 tttgattgaa atttttttta ctagaaacag ttaaagtgat taataacaaa acagtcataa
    16321 ataaataaaa tagttctctt gtcttattgt atcttctatt tttgaaaaat atcatcgtta
    16381 atatcagaga agagaatatt atttgcaagc ttaagatccg ccaaggaaat tgtaacaatt
    16441 tggctggcgt attttgcaga agataccatg gcaccaaact agttgtagct agccaagtca
    16501 aacacgtacc ataataaatc tttttttcta tgtttgttaa ttttttaaat aaaatcaggg
    16561 gaatacataa agataacaga ccaactagtc ccattgagta agtagttaag tcgttgttca
    16621 tagcttcagt cagactatcg aacacattag atgcatgagc atataaattt gtcacgctag
    16681 gtttgttgat tttttggtag agcatttgtt caaacatcgg ataccaaaaa gcactcgtca
    16741 gaaccaatgt cattcctgcc gcactaaata attttttaaa tatagcaata aaatctgcat
    16801 tcttgaaacc tttagaaacc aaacgaataa aaataaaaat gataattaat gctgtactca
    16861 ttaatgttga tagtacatgg ctgtacacta gcaaactcat actcaatgct agctttacag
    16921 acggatactt ttcttctttt ccagagatga tttgataagc ataaaataat acagggataa
    16981 caagtgttat tgcgatagct tcggcaattg cggaacgata gtagatgtct actgtccgat
    17041 aagcagagaa aatatagaga caactaaata aaaatgccgc ttttttttgt tttaaaaacc
    17101 gctcgccata atgataagct acgagtgttg tacacaagtt caataaccaa acgtaaataa
    17161 tatagcttac aatcaaattt tttgtcatcc aatataacat tacagctgga aaaaaagtca
    17221 gaaagggata aaaataattt acaccatatc cagtataatt gaatgtctca taattaatcg
    17281 gactggtaaa aatagtcgat aaccccttga ttcgagataa gtgaaaagaa agatctccag
    17341 tggtataaat actctgtgta tttttgaaaa agatgaaaga atatatacta ctaactataa
    17401 ttaatccact caatattact acttttttat ttattttcat tcttcttgtt tatctccttt
    17461 ttagtcacat atcgagaatc tttcaactta ttaggtgcaa atcgtttaaa acttgaatac
    17521 accactaaac aataagtcag aatactgatc cataaagcca tgagagcagt tggagtatat
    17581 tttgttgtaa tttctatctt attttctcca gcatttacat ccacattcac taagccatgt
    17641 tttttataag agtcatttac gactttaccg tttactttta cctgtgtacc taaataagca
    17701 tatactggta ctgtcacttt ttgctttttg ttgcttgcca ctgtgtaagt aatgttatta
    17761 tctgcagctt tatacttccc tttttcccac gattctccta cttttacttc atgtttttct
    17821 aagtgaggcc tttcttttat cgcttctgtt ggggcgtaat cgtataatcc tcccatatct
    17881 gatgttgtat aacgtgggag tgtttcttca ttgataacaa tatgaccgtg ttttgattgg
    17941 ataacttttg cgtaattttc ttttgctgct atagacgtga cgagtagaaa caccacagta
    18001 gcgccaatga ttaaccatga atatttttta ttcatagtct ttttcataaa aacaagcgtt
    18061 atgatcattg aagaaaaaaa gatttggata cctaagatac gccaaggaaa ctggagtaag
    18121 cttatcggtg ttttttgaaa caaaaaccat ggaaataaat tagttacgca tacccatgta
    18181 gaaactacgc caacatagat gattttttct tttttctcca tttctttcag tatgaataaa
    18241 ggaaaaatca acgcaaccaa tcctaataac cccatagtgt atgttgtaat atcgttattt
    18301 aacgcttgaa tgatactatc tgaaatagaa agtgcttcta gctgcaaatc tgttttgaag
    18361 ggactgtgta tttcttgata attcatctgc tgcaacatag gataccaaaa atagctagtt
    18421 aatgcacaag tcaaaaaaac agcctttact aaattcacga ccacaaattt tatatcgttt
    18481 tttatttgct tgcttaaaaa caaacgaact ataatcatac aagcaatcaa tgcactacac
    18541 aatagtgtag ttaagacatg agtatacaca agtagactta tactgacaga taaagcgatg
    18601 actttttttt ctctattgaa tatcaatcca taagcataat ataaaacagc tgggataatc
    18661 gtaagagcga ttgcttctgc aattgcggat cgataataga tatcaactgt tcgataagtt
    18721 gcaaaagtat ataaacaact aaa
//
LOCUS       DANKAZ010000049        18740 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000049 DANKAZ010000000
VERSION     DANKAZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            256..531
                     /locus_tag="PWY67_001653"
     CDS             256..531
                     /locus_tag="PWY67_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057925.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="PWY67_001654"
     CDS             544..2616
                     /locus_tag="PWY67_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL2057926.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="PWY67_001655"
     CDS             2781..4667
                     /locus_tag="PWY67_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2057927.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="PWY67_001656"
     CDS             4679..5626
                     /locus_tag="PWY67_001656"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL2057928.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="PWY67_001657"
     CDS             5646..6173
                     /locus_tag="PWY67_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2057929.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="PWY67_001658"
     CDS             complement(6236..6889)
                     /locus_tag="PWY67_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL2057930.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="PWY67_001659"
     CDS             7021..7863
                     /locus_tag="PWY67_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2057931.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="PWY67_001660"
     CDS             8021..8917
                     /locus_tag="PWY67_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2057932.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="PWY67_001661"
     CDS             8920..9633
                     /locus_tag="PWY67_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL2057933.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="PWY67_001662"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="PWY67_001662"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2057934.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="PWY67_001663"
     CDS             10168..10395
                     /locus_tag="PWY67_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL2057935.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="PWY67_001664"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="PWY67_001664"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2057936.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11681)
                     /locus_tag="PWY67_001665"
     CDS             complement(11181..11681)
                     /locus_tag="PWY67_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2057937.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="PWY67_001666"
     CDS             complement(11960..12358)
                     /locus_tag="PWY67_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2057938.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="PWY67_001667"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="PWY67_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL2057939.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="PWY67_001668"
     CDS             13471..14238
                     /locus_tag="PWY67_001668"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL2057940.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="PWY67_001669"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="PWY67_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL2057941.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="PWY67_001670"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="PWY67_001670"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL2057942.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="PWY67_001671"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="PWY67_001671"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL2057943.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DANKAZ010000050        18285 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000050 DANKAZ010000000
VERSION     DANKAZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            45..332
                     /locus_tag="PWY67_001672"
     CDS             45..332
                     /locus_tag="PWY67_001672"
                     /inference="COORDINATES: protein motif:HMM:NF025015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2057944.1"
                     /translation="MKNNPPTKINSFYGFRTKKSMKNEKSWHLAQKLLGDFSKKKVIP
                     VFLMGIVFFSIEIYSLAILKNENFFVSVLMGESLILILILCLIVHEVNKQL"
     gene            756..1019
                     /locus_tag="PWY67_001673"
     CDS             756..1019
                     /locus_tag="PWY67_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057945.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            1353..1724
                     /locus_tag="PWY67_001674"
     CDS             1353..1724
                     /locus_tag="PWY67_001674"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2057946.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            1904..2326
                     /locus_tag="PWY67_001675"
     CDS             1904..2326
                     /locus_tag="PWY67_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057947.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            2471..2716
                     /locus_tag="PWY67_001676"
                     /pseudo
     CDS             2471..2716
                     /locus_tag="PWY67_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2825..3781
                     /locus_tag="PWY67_001677"
     CDS             2825..3781
                     /locus_tag="PWY67_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057948.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(4617..5288)
                     /locus_tag="PWY67_001678"
     CDS             complement(4617..5288)
                     /locus_tag="PWY67_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2057949.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            5605..7236
                     /locus_tag="PWY67_001679"
     CDS             5605..7236
                     /locus_tag="PWY67_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2057950.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            7239..8318
                     /gene="uxuA"
                     /locus_tag="PWY67_001680"
     CDS             7239..8318
                     /gene="uxuA"
                     /locus_tag="PWY67_001680"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2057951.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            8392..9819
                     /gene="uxaC"
                     /locus_tag="PWY67_001681"
     CDS             8392..9819
                     /gene="uxaC"
                     /locus_tag="PWY67_001681"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2057952.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            9903..10550
                     /locus_tag="PWY67_001682"
     CDS             9903..10550
                     /locus_tag="PWY67_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL2057953.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            10679..12055
                     /locus_tag="PWY67_001683"
     CDS             10679..12055
                     /locus_tag="PWY67_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2057954.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            12066..12860
                     /locus_tag="PWY67_001684"
     CDS             12066..12860
                     /locus_tag="PWY67_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2057955.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            12873..14639
                     /locus_tag="PWY67_001685"
     CDS             12873..14639
                     /locus_tag="PWY67_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL2057956.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            14667..16475
                     /gene="uidA"
                     /locus_tag="PWY67_001686"
     CDS             14667..16475
                     /gene="uidA"
                     /locus_tag="PWY67_001686"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL2057957.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            16647..17048
                     /locus_tag="PWY67_001687"
     CDS             16647..17048
                     /locus_tag="PWY67_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2057958.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            17042..17287
                     /locus_tag="PWY67_001688"
     CDS             17042..17287
                     /locus_tag="PWY67_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2057959.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(17611..17832)
                     /locus_tag="PWY67_001689"
     CDS             complement(17611..17832)
                     /locus_tag="PWY67_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057960.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(17994..18066)
                     /locus_tag="PWY67_001690"
     tRNA            complement(17994..18066)
                     /locus_tag="PWY67_001690"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18031..18033),aa:Lys,seq:ctt)
     gene            18142..>18285
                     /locus_tag="PWY67_001691"
     CDS             18142..>18285
                     /locus_tag="PWY67_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2057961.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIG"
BASE COUNT         6395 a         2494 c         3592 g         5804 t
ORIGIN      
        1 ttttattgat tttgtctctc gtattactag ttgtatctaa tctaatgaaa aataatcctc
       61 ctacaaaaat taattctttt tatggcttta gaactaaaaa gtcaatgaaa aatgaaaaaa
      121 gttggcattt agcacaaaaa ttgttggggg atttttcaaa aaaaaaagtt atcccagtat
      181 ttttgatggg tattgttttt ttcagtatag agatttatag tttagctatt ttaaaaaatg
      241 aaaatttttt cgttagtgta ttaatgggtg aaagtttaat cctaatactg attttgtgtt
      301 taattgttca tgaagtgaat aaacaattgt gaattaaaaa aataaaaacc tagtataatc
      361 actttaataa attgttggta gaaggtgatc aaaatgggag taaacgaaat gatgaaagtc
      421 agattgagtt tttaaatgat accttcctca gaacttcaaa acgaattcaa agattcgttg
      481 acaaatcttg agcaagatca tttaaaaaaa tattttctct tcatttgacg aaaaatgttt
      541 agaaatctta tataagtgaa gataaagtaa ttggtgtaat taattgtaga gaatagcctt
      601 tgagcaattg aagcgataca attatttaga aaatgaactt cctccagcat actacatgat
      661 tccttacggg acgatgttaa agaagaaagc aagaaatttt agaagtatta gtagataaac
      721 taatgggctt ttctcaattt aagggagtag cccaaatgtt tatcaaatat ggtgcagata
      781 cagtagcaac catgtttaca gaagaatatc aagtagacta ttatgaagta atagataaat
      841 gtgtacattt tgtttatgaa aaagtgtgga attttatgaa ttttgcgata agtgaagaaa
      901 tcttacgagt acaagaaaac agaacaggaa tatttaccag agacagaaag cttaatttga
      961 ttgcgcatta tatacggaat cgctggtcat caataccaaa ttttaattat aaaaaataat
     1021 tatgaacaat cttttataca atatacacaa taaatcaagt agtatttatg aaaaaagact
     1081 tcaaaaacag aatgattttc tatcagtctt agaaattata gagctaaaaa ttttggtcac
     1141 gcgtagaaac gcgttttttt tgtttttttg atagtaaaga acgtacaaaa caatgagaat
     1201 ttaaaagatt gtactaccag tttttttacc ttttcatttg agtttagagt aagtaaaata
     1261 ttactataaa aacacatatt cttgaagaaa tctttttttt gctgtattca tttagattat
     1321 gatagagaaa atacgtgaga gggggacctg ggatgtggct atctgatgaa aaaattcagt
     1381 ggcgtagcta tcagaagcgt atattgacta actttgctac ttatttaaat aacaattcag
     1441 tccatctagt ggctccacca ggggctggca agaccttttt aggaattgag atgatccgac
     1501 gtttggattg taaaacctta attttggttc cttccctaat attgaagaaa cagtggttaa
     1561 caacaatcga ggaatatttc ttagtaaatc cagcagatag ttgctatctt tctggggcga
     1621 ttgaacatcc tagtcatatt acagtggaaa cgtatcagac catttacaac cgactgaagg
     1681 aggagccaac ttatggtgat cagctggagc tgttagtttt ttgatgaagc tcatcattta
     1741 aaaaaaagct ggggtgaatt gttgattgac ttgaagaaaa aacaaccgaa tctgttgacg
     1801 atttccttga cagccacgcc tccgattgat agctctcaga cggagtggaa gcaatatatt
     1861 gagttaaaat ggcctgattg acgaagaaat tagcattccc gagttggtaa aggaggaggt
     1921 tctatgtcct catcaggatt ttctatattt gattccggct aatcaggaaa ctactcagca
     1981 ttattttacc tttttagaga aacaaaatga gctggtggaa gcgttaatgg aaagccaaga
     2041 gacggtggat tatttgcttt ctttagaatt tgttcagcat ccgcttgagc attcgaagga
     2101 aatttatgaa aattttgagc tgttcactag tgcattaatt tacttgaatc atcataacta
     2161 tgaattgata cccgaacatt ggcagctatt aggcagttct aagcagcaag gtaaaaaagc
     2221 ttcagttcca tctttaggta aaaaagagct gatcactatt tatcagcaga tgtttgaaga
     2281 tcaaccggaa ttgcctattt tttatcaatt gaaaaaagct ggatgattgt ttgatgagtc
     2341 gctgcatttg tttccagagt atccagacac tgttggtagt caggcgattc ctgagaaaaa
     2401 gcaagcagtt tgtcaaattt tgttaaaaga agaagttcag ctgaagcaag aactgacagc
     2461 ggtcattttg atggatcgta tttgcaagga ggtattagca ggcagtgatg gtaccttagc
     2521 ttatggcgtg gttcctttgt ttgatgaagt tgttcgtcta ttaggtaaca ctcagcaaat
     2581 cggtgccatc tgtggagagt tcttactgtt accgaaaatg ctagtaactg ttgagctggc
     2641 agaactagcg acgggtatga atgaacatcc ttatatgtcg ggttattttt atttgacggt
     2701 ggatgacaaa aaccgccaag cattgatgca agcagttact cgcctattaa ccgaaaagaa
     2761 gttgaatatt ttggtaggaa ccgttagttt gctaggagaa ggctgggatt gtcctgcaat
     2821 taatctgttg atcttggcta atcaagcttc cacttttgtg caaacacaac aactacgagg
     2881 gcggggctta cgcctaggtg cacaaaataa agtaacgaac atttggcatt tagcgattgg
     2941 attaccggga gttcctctgt ctgaacagcc tgatttgaaa agaatgctac ggcgtttggg
     3001 caatatttca ggtctgacct ttgaatcttc accaaaaatt gagagtggtg gcgatcgctt
     3061 tgctttacca ctggaatata cgaccaccac gatggaaact tatactcagt cgatgttcta
     3121 tttttcttgt gaacgaagtc ggttgaaaga acaatggagt actgctttag ctgatggctg
     3181 gcacctatct atgccaatcg tcgttaggaa agtcgatcag gtaaagcaaa aacaagaaac
     3241 ttcattgccg atagtagtaa aaaatataga gcctctaagc ttctggtcag ctatatttaa
     3301 aggtaacttg ctggaatatt tccaaattcg taggcgacaa aagctatggg aaaaggaatg
     3361 tgcccgcaaa aaaagcttgg ccatatgcgt tcaaacgacg ttgcagcgcc ttggtgaagt
     3421 tagtgataga aaagtaatcg tagcggctga tcagcaggaa tttagatgct atcttgagaa
     3481 tgctggctat cgagaaggac agctttttac tgaagctatg agggagcttt tagcaccgct
     3541 ggagaatccg cggtatttaa tcgaagggaa aaagatagtt ctgacggtac cagaacgctt
     3601 tggccgaaat aagcaaatcg ctaacctttt tctaaacgaa ttaaatcaat cgtcgctgcc
     3661 caaatgtcag ttggtgtata ctagaaatcc tgaaggacgc caaaagctag tgacttatcg
     3721 aatgaggcaa gtcttccaga cccgcgatga agtaattatt caaaaaaaca tttggacgta
     3781 agttgatgaa aataaatttt gctagagtag aacaagtccg agaattaagt tgctacttaa
     3841 ctgataatgc caaggacagg agaatctttt cttgacgact gaaagaaact atttgactta
     3901 tataacggtg tgtgatgaat atttgcaagg cattctctta aaaagtactc gtaatagttt
     3961 ttttgaaaac aaaacagtac tttattaata ggactgacag agcgatataa gaacatctgc
     4021 agacagaggt gaatgctttt ctcatgatcg atgaaacaat ggtacttcgg atattcttgc
     4081 ttttagtttg aaccgttgtg attttctttg attcctaaaa gtagttttat agaatgaaaa
     4141 tcaagcatac ggaatgctcc tttgattgtg ttgtggtgat tcaatctaat ggagcttcta
     4201 tatgtttttt tatttgcaaa aagaaccgat agaatatttt tatcagtctt agaaattata
     4261 gagccaagaa atttcaagca cacataaaaa cgcgtgtttt atgcatttta tgagcaaaaa
     4321 taactaaaga ccattacgta aaacataggt aaatcagcat tcaatctcaa attatgtgat
     4381 caaatgttga tattatttga ttataagttt tcttttgcta ttatcagtct gcaaataaac
     4441 agattataga gtatgaatgt agatagttca atctttgttt acgacctcac atgttttttt
     4501 actcaagaga aagtaatata aagtgactat atttgacaga actttacata aaaaaagtag
     4561 ttagttttct gtagtaatca gaaatgtaaa taaattaaaa aaaagaaatt tagcagttat
     4621 atatttttaa aatattttgg gtgagtagtc aatacaacat tttgttcatc aagcattaaa
     4681 tgaagatgca aggatgttaa gaagttagct tcttcagggt cttgcatggt caaggcttta
     4741 aatagttgat gatgttgttc cataattttt tcccatttta atccttccgt ttttactcgt
     4801 aaccaacgaa atcgttccaa gtgcgtatta ttatcttcca accattgcca tacttcttcc
     4861 cgttctgcaa tttcaaaaca agctttatga aataaattat cattatgaaa aaaactacga
     4921 gcattctttt gactaacagc tttttcttgt tctgcaatta tagtttctaa aacttgtttc
     4981 ttttgattat tcatcttggc acaacactct agcataattt gtcgttctaa atgttctctg
     5041 acaaaacgag cattttttgc taaatctaag tcgattaatg agatataatt tccactttga
     5101 ggaattgtat caacaagtcc ttgatgacgg agttgaatca atgcttcacg tattggagta
     5161 cgaccgatag aaagcgtttc ttccaatttt ttttctgata ctttttttcc aggttctaag
     5221 tcagcgtaga taatttgctg acgaatagat tgataagctt gattttggag actagtttta
     5281 attgtcatat ttaatccctc tcaaaatttc atttttgaat ttgatatttt agttctatgc
     5341 tcgttatata atatcaaatt tcaaagtaac atactagcta ttaaaaaaac aaaaaatact
     5401 caaataagat caaagagaaa ttaagcttta atttaagtta gttctaaaaa atatatattg
     5461 gtgtgctttt actaaaaaaa gacacaataa ttctgacatg gtagaaaaaa gaatattttt
     5521 agttttttct tattgacagc gattacattc gattttatac tacaattgta acggaaacta
     5581 acatatcagt atggaggggt aaatatggtt ttgattaaag actggcaaga ttctatcgaa
     5641 ggattgaaca aaatgcaagt tcaactaccg tattttaatg tcgaagcggt caaacaagaa
     5701 ggtcagaaac atccagtgtg gcttcatttt ggtggaggaa atctttatcg aggatttcat
     5761 gcagaaattg ctcaatgctt agcagatcaa aatgaattag aatccggagt agttgtgtgt
     5821 gagacatttg atgaagaagt agtagaaaaa gcttatcatg actacaacaa tgatattttg
     5881 gagattgtta tgggtgaaga tggtgaactc aaaaaaagac ttttagcagt aacagctgat
     5941 agtttatatt gcaatccaaa gaatataacg gactatcaaa gagtggttaa atattttgaa
     6001 gatcctacat tacagtttac aacgtttact attactgaaa aaggatacag tttaaaagat
     6061 tctaaaggac aatttttttc ggtagtggaa gcagatataa aatctggacc taccaatcct
     6121 caacatacga tgagtattgt gacatccttg ttgtatagca ggtttaatgc tggagagtta
     6181 ccgattgcga tggtttctac tgataatttc tcacagaatg gtcaacgttt tcaagatagc
     6241 atttttatga ttgcagaagc ttggcaaaaa aaaggatacg tttctgaaaa atttattgct
     6301 tatttaaagg ataaagaaaa agtgactttt ccttggagta tgattgatag aattacgcca
     6361 aatccttcgg aaagtgtcaa aatggattta ttgaaggcgg gtttcgaaga cttagatata
     6421 attcatactg cgaaaaatac caatatcgct gcttttacca atactgaaaa agtccattat
     6481 ctagtcattg aagatagttt tccaaatggt cgacctaaac tggaagcggc tggtgtgatt
     6541 atgacagata gagaaacagt tgataaagca gatatcatga aggttacgac atgtttaaat
     6601 ccgctccata cggcattagc aataacaggt tgtttgttgg ggtataaatc aattcaggca
     6661 gaaatgcaag atgatgattt ggttggctta attaaaggga taggttatgt agaaggctta
     6721 ccagtagttg aaagtccagg cgttattgat ccaaaacaat ttattgatga agtaatcaat
     6781 aaacgtttgc ctaatcccta tattccagat acaccgcaac gaattgcaac agatacttct
     6841 caaaaagtag cgattcgcta tggtgaaaca attaaaaagt atgtcgaaaa agacggaaat
     6901 gcagaaaatc taacatttat tcctatcgct attgctgctt ggttacgtta tttattggca
     6961 gtcgatgatc aaggaaaaaa ttttgtgcca agtcctgatc ctttattggt agagttgcag
     7021 gaaaaattct cagtattgaa attaggtgaa caagatcctg caaagattca ttgtgcggta
     7081 caggaaatct tagaaaataa ggaaatattt ggtttagatt tatatcaagt tgagatagga
     7141 aagaaaattg aaaaaatctt ttgcgaaatg ctagtaggtc ctcaagcagt tcgtaaaaca
     7201 atcaataaat atataacaga agttggagga aagtaactat ggaaatgact tttagatggt
     7261 atggggcaaa tgaagaaaaa atctcactac aagaaattcg tcagatccca gcaatgaaag
     7321 gaattgttgg aacattaatg gatataccag ccggagaggt ctggccgaga gaacgaatta
     7381 aagctttaaa agatgaagtc gaagccaatg gccttacctt gaaagttatt gagagtgtta
     7441 atgttcatga tgatatcaaa attggtttgc caacacgaga tcaatacatc gagaattata
     7501 aacagacgat tcgcaatttg gcagaatttg ggattgaggt tatctgttat aattttatgc
     7561 cagtctttga ttgggtaaaa tcagatttgg attatcattt agatgatggg tcttctacgt
     7621 tagcctatat ttcaaaaaat attcctactg atccagagga aattattaga aaagtagaaa
     7681 attctactaa taattttgaa ttgccgggtt gggaacctca tcggttatca caaatcaagc
     7741 aattattaga agcctataaa gaagttgatg aagcgaaatt gcgagagaac tttgcatatt
     7801 ttctcaaagc aattatcccg acatgtgaag aagttgggat taaaatggca gttcatccag
     7861 atgatccacc atattctatt ttcggattac ctcgtattgt taaaaatcgt gacgatttag
     7921 aatggttgtg taatgtagtg gattctcctt ctaatggtat tacgttatgt actggaagta
     7981 ttgcagaacg accagataat gatgtctatg aaattctatc tgaattttgt caacgggatc
     8041 ggattccttt tgcacatgtt cgtaatatca aatatttaag tgataatgga ctcgattttt
     8101 atgaagcgcc gcataaatca gaatatggtt ctctagatat gtataagata ttaaaaacaa
     8161 tgtacgataa tggatttaat ggctatattc gtccagatca tggtcggatg atttggggag
     8221 aaaccggtag acctggttat gggttatatg accgagctct aggtgcagtt tatttaaatg
     8281 gtatttggga agcgcttgaa aagggggcga gtaaataatt atataatgga gtctatttag
     8341 aagaaaaatt ttctcttcta aaaactaagg gaaggaaagg agtgcttaaa tatgttttta
     8401 gataagaact ttttattaga gaatgaaata gctaaaagac tttttcataa atttgcagaa
     8461 caccaaccaa tcatcgatta tcattgtcat ttagacccag catgtttgct agaaaataag
     8521 caatacaaga atttgactca ggtatggtta aatgataaag gagctggaga tcattataaa
     8581 tggcgattga tgcgagctaa tgggatagaa gaagagttga tcagtggtgc aggtaatgat
     8641 tatgaaaaat ttttagcttt tgtaaaaaca gtggagaagg cgccaggtaa tcctattttt
     8701 gaatggtctc atttggaatt acaacgttac tttgatattg atttgacaat ttctgagaag
     8761 aatgcaccag aaatttggcg acgtgccaac gaaaaaatcc aaacggattc ctatcgccca
     8821 aaagaattgc tgaagatgat ggatgtgaaa gccatttgta caacggatga tcctacagat
     8881 gacttgcgat atcatactga gctttctaaa caggataatt ctttcaaggt attacctact
     8941 ttccgtccag ataaggtctg gagtatcaaa tcatcaggat tttcgaacta tattttaaaa
     9001 cttagtcaag cagcgggtat accaatcaaa aactttaatg ggttgaagca ggcattgagc
     9061 caacgagtgg attattttca tcaaattggc tgtcgattag cagatcacgg tttgaatact
     9121 tatttttatg aaatagcaga tgaaaaaata ctcgacaatc tgttaataaa agctttagca
     9181 ggaaaaacgg atttccattt atcagaaatt gcacaatttg ttacaggtat tcaattacat
     9241 ttgatgtctg agtatacaag atatggttgg acgatgcaaa tgcatttaaa tgctttaaga
     9301 aataattcaa gtatattaaa ggaacaaatt ggagtagatg tcggtggtga ttcgatgggg
     9361 gatcaagcta gtttgacaca aaatgttgta gcattgttcg acactgctga aaagagtggg
     9421 tgcttaccaa aaacaatttt atattcatta aatccaaccg attggcttcc actagcaaca
     9481 gcaatgcaaa gcttccaagg gggtatgatt caaaaaatcc aattgggatg tgcttggtgg
     9541 ttcaatgata cagctgaagg aatgcgtcaa cagttgacta cgatggcgca acaaagtttg
     9601 ttgtcgaatt ttacaggtat gttgactgat tctcgaagtt ttctaagcta tcctcgccat
     9661 gagtatttcc gtcgagtatt atgtcaatta ctaagcgagt gggttaaagc aggtcgaatt
     9721 ccagatgatg aaatgtatct tagtcagata gtggagaata tttgttatcg taatgcgtat
     9781 aattatttta atttttttga aatcgaaagt agaaaataat acaaaaaata aaagcttaaa
     9841 tttatttatt gtaagttagt acaactagct agagtatgta tccataaaag aaggagatag
     9901 atatgaaaaa agtagaaatt ttaagtaagt tagaagcggc tggagtcatt gcggttgtac
     9961 gtggaaaaaa taaagatgag gccattaaag caataaatgc aattactgcg ggtggaatta
    10021 aagggattga actaactttt acagttccga atgctgaaga aataatcaaa gaaattgcta
    10081 aaaaatatgc tgatcaaaaa gatgtaataa ttggagccgg tacagtttta gatgcgttta
    10141 ctgctcgtat tgcaattatg gctggagcag aatatatcgt aagtccatct tttgatcgac
    10201 aaacagcaga gatttgtaat ttatatcaaa ttccttattt accaggttgt atgacaatta
    10261 ctgagataaa ggaggcactt accgctggag tagaaatcat taaactattt ccaggttctg
    10321 cttttggtcc atctgttgta aaaacgtatc aagctccatt accatatgta aatataatgc
    10381 ctactggcgg agtaagtcta gataacttag aagagtggtt tgaagcaggc gttgttgcag
    10441 ttggtgtagg tgggaatttg ttggctcctg ttgaaaaagg tgattttgaa gaagtaactc
    10501 gaatagctag taaatatata gataagtttt atgagataaa gaattattag gagaaaaaat
    10561 aaataatact ataaaatgta aaaaataaat actaactctt tatacttcag agtatagtac
    10621 gtaagttggc agttcaacag aatgaaaacg cttaacaata ggaaggagcg caaattatat
    10681 ggaaagaaaa tttggtctaa gagacaaaat aggatatatg tttggagatt ttgggaatga
    10741 tttcactttc atttttgcta gttctttttt aatgattttt tatacaaaag tattggggat
    10801 aagcggagct accgtaggaa cattattttt agtagcaaga tttattgatg cagttacaga
    10861 cataacaatg gggcgtattg tggatggatc aaaaccatct aaaactggca aatttaaacc
    10921 gtggatttta cggatgagtg gtccagtcgc tttagcaagt tttttaatgt atcaaacatc
    10981 aatggcatca gcttctatga gtattaaagt tatatatatg tatgtaacat atatactatg
    11041 gggaagtatt ttttatacat ctattaatat tccttacggt tcaatggcat cagctatcac
    11101 ttctgttgca gaagaaagga cagcgttatc aacatttcgt agtataggag ctaccttagc
    11161 aggtatgttc attggcgtgt taacaccaat ttttatttat acaagagata gttctggagc
    11221 tcaaattgta cgtggtggat caacttttac aattgtggca ggagtatttt cagtgtgtgc
    11281 agttatttgt tacgtaatat gttataaatt aactgtagaa cgggttaaaa gtgatgaatc
    11341 gcagttaaaa gtttcattgt tgaaatcttt aaaaggatta gctaaaaata gagcgttgct
    11401 ttctataata gctgcagcta tttttttgtt gcttgctcag cttttaacaa tgagtatgaa
    11461 caattatgta tttccagatt attataataa tgcacaggga atagttttaa tgaactttat
    11521 caatcctatt ttgatgttgg tacttgtttc acctgtaagt ttaattttca gtaaaaaata
    11581 cggtaaaaaa gaattggcgt cagtagggat gttcttctct gctattgtat atttactttt
    11641 atttttgata aagcctagta atatgtatgt attcttagtt cttacttctg ttggatatat
    11701 ggggttaggt atttttaata cagtcatttg ggcgaatatt actgatgtaa tcgatgatca
    11761 agaagtgaaa tctggccaaa gagaagatgg aactatttat gcagtatatt cttttgcccg
    11821 aaaaattgga caagctttag ctggcggtgt cggaggttgg actctttcta taatagggta
    11881 tgattcgtta gcaaaagtac aaagtggaga agttttacaa aagctgtata actcttcaac
    11941 gttgattcca gcagtttgtt tctttgttgt aggattgatt ttatttttcg tatatccatt
    12001 atctaaagaa aaagtattag aaaattataa tttgctaaat caaaaacata actaggagaa
    12061 aaaacatgaa cagttggata ttttgtattg attcagatgg ttgtgtcatg gatacaatga
    12121 cttataaaca caatctcttt ttcgggccaa ttgctgcaga gatttttgat gttcatcata
    12181 gggaaaaatt cctacaggat tggaataata ttaatttata ttcagagact cgtggaataa
    12241 acagattttt aggtttagtt aaaacattag aaaaaaatga tgtaccatcg attaatcgat
    12301 taaagcactg ggtatctgta acaaaagaat tgtctaatcc atctttgaag agggaaatag
    12361 aatactctca gtcagaggat ttattaaaag ctttaaaatg gtcggaagaa gtcaatcgac
    12421 agattagtca agctaaagga ttagataaac cctttttaag tgctagagat actataaaag
    12481 ctctgaaaaa atatgggaag ataattattg taagctcagc taataaggaa gctattcaag
    12541 aagaatggga aagacatgaa ttattatcct tagttgatga actgtgttgt caagataaag
    12601 gaaaaaaaga agacattatt cgaagtgtaa ttgaaaatgg ttgtgatttg gataagatat
    12661 taatgattgg tgattctcct ggtgatctag aagctgcaaa taagaataaa gttttctttt
    12721 atcctatttt ggttaataaa gaaaaagaat catgggaaaa cttaagaact gatattttat
    12781 ttaaatgttt atcgggagag tatatagata ttgaacaaaa atatatagat actttttgga
    12841 aaaatcttac taataagtag aggagaaaga aaatgactat aaaagtagac ttacgaaaaa
    12901 aaccatatca tttagatgaa gatgctattt cttggattga agagacgatt gatgctatga
    12961 ctttagaaga aaaaataggt caattatttg ttaatatggg atctagtcgt gataaaaatt
    13021 acttaagtag tatggtcaat cagtaccata ttggagctgt acgatataat ccaggaactg
    13081 ctgatgaggt atatgatcaa aactatatcc tacaaacaga aagcaaaatt cctttattaa
    13141 ttgctgctaa tacagaagca ggtggtaacg gagcttgtac agatggtacc gaagttggtg
    13201 tagaagtaaa aattggtgca acaaatgatg cgaaatatgc ttatgaatta gggagagttt
    13261 ctggaattga agcatcggct attggatgta actggagttt tgctcctatt gtagatatta
    13321 atagaaattg gagaaatcct atcatttcta gtcgatcttt tggacaaagc cctgataaag
    13381 ttttagaact tagtttagct tatatgaaag gtattcaaga gagtggaatt ttgcctgcag
    13441 ctaaacattt ccctggtgac ggtattgacg aacgagatca acatctatct tttagtatta
    13501 atagtttaag tacagaagaa tgggataatt cttttggaaa agtatactca ggcttaatag
    13561 aagctggatt accttcaatt atggcaggtc atatccatat gccagcttat tcaaaacatt
    13621 ttaatcttga tctgaaagat gaagatttac ttcctgcaac tttatcaaaa gaattaatca
    13681 ctgacttact tagaggaaaa ctcgggttta atggattagt agttactgat gctagtcata
    13741 tgttaggaat gactggggca atgaaacggt cagaattatt gcccacttct attgcggctg
    13801 gatgtgatct attcctattt tttaatgatc cagatgaaga ctttaattat atgatggaag
    13861 gttattacaa aggaattatt tcagaagagc gtttacgtga tgcattaaga agaattttag
    13921 gcttaaaggc taaactaggt ttgcataaaa aagaaaagaa taaaattttg ttaccgaaac
    13981 aagaagctat ggaaaaaatt ggtttaattg aaaataaatc aatttttaaa gaggtagctg
    14041 atcaagcaat tacactagtt aaagataaac aagatatttg gcctattgta ccagaaaaat
    14101 ataaaaggat tttactggta gatgtacatg gggtaaaagg tgggtttggt gaagtagttg
    14161 ctggaaatag acctcaggcc attgatttat tgaaacagat tttgtcagca aaaggatttg
    14221 aagttgaaaa atacatttca cctgaagaag aattgaaagg attatcgcaa gaagaaaaaa
    14281 ttaaaaatat agctggaata tatgctgcta agagaccaat caaagattta actgaaaaat
    14341 atgacttgat tattaatata gctaatgtta acccaggtac agttcaaaga attgtttggc
    14401 cagcaagtaa aggtactccg gatattccat tttatattca tgaaattcca acaatttttg
    14461 tatctgtaca gtgtccgttt catttagctg atgtgcctca agtaaaaact tatatcaata
    14521 cttatgatgg aaaacaagaa actttagaaa tattagtgga caaactaatg ggcctttctg
    14581 aatttaaggg aataagtcca atagatgctt attgtgggtt aaaggataca tttatttaat
    14641 atttaaatta tataggagtg atttacatgt tatatcccat tatgacagag acgaggcaag
    14701 taattgattt aagcggtctt tggagattta agttagatac aaatgatgac ttacaagaag
    14761 aacttgcttt agaaccaatg gttgaaaaag agagttatcc aatttcagtt ccttcttctt
    14821 ataatgattt atttgaaaca gagaacatta gaaatcatgt tggatgggtg tggtatgaaa
    14881 aagaagtgat tcttcctaaa aaattattag aagaaagatt agttcttcgt tttggatctg
    14941 caacacatga agcaaaagta tttttgaatg gaaaattgat taatcaacac aaaggagggt
    15001 ttacgccatt tgaatcagaa attaatcagt ttataaaaga tggaagtaac agaattacag
    15061 tggctgtaaa caatgttatt gacgatagca ctttacctgt agggattttg aaagaaacga
    15121 tacaggaaga tggagacata aaaaaagaaa atttggttaa ttttgacttt ttcaattatg
    15181 caggcattaa tagacccgtt aaattgtata caactccgaa agactatata aaggatgtca
    15241 ctattaatac aaaaataatt gattcagatg caaatgtaga atttaatatc gatacagaat
    15301 ggaccggaga aattctaata gcaatctatg acgaagaagc tcaacttata gcagaagcta
    15361 aggggaaaga aggagtgatt caacttaaga acccaacact ttgggagcct ctaaatgctt
    15421 acttatatag tatgaaagtt agtttaatta acgaaaataa agtgattgat acgtacacag
    15481 aagaatttgg cattcgtagt attcaggttt cgaatgggaa gtttttgatt aataataaac
    15541 ctttttactt taagggattt gggaagcatg aggacacgta tattaatggt cgtggtttga
    15601 atgaagcagt aaatatcaaa gattttaatt taatgaaatg gatcggtgct aattcattta
    15661 ggacttcaca ttatccttat tcagaagaaa ttatgcgctt agcagatcga gaaggtattg
    15721 tagttattga tgaagtacct gcagtaggat tacatttgaa tttcatggct actttattag
    15781 atgacaacgt agaaaaacat gatacttgga aagaaatacg gacagaaaat gctcacaaaa
    15841 tggcgattaa ggaattgata gaacgcgaca agaaccatcc gagtgtcgta atgtggtcta
    15901 ttgcaaatga accagattcg gattctgaag gcgctaaaga atattttgaa cctttagttg
    15961 aattgactaa agaacttgac cctcagaaga gacctgtaac aatcgttact tacttaaagt
    16021 caacaccaga tatttgtaaa gtaggagata ttgtcgatgt tttatgttta aatcgctatt
    16081 atggttggta tgtttcaggt ggagacctaa aacatgcaaa atatctgttg aaaaaagagc
    16141 tagatggatg gatggaaaga tgtcccgata cgccaataat gtttacagaa tatggagcag
    16201 atacagtagc tggacttcat gatacagtac caacaatgtt tacagaagag tatcaagtgg
    16261 gttattacga agcaaatcac gaagtaatag ataaatgtga caattttgtt ggtgaacaag
    16321 tgtggaattt tgcagatttt gaaacaagtc aagggatttt aagagtgcaa ggaaataaga
    16381 aaggaatctt tactagagat agaaagccta aaatgattgc tcactatcta aagaaacgat
    16441 ggtcatcaat accaaatttc agttataaaa aataatttac cttttataag gaatataaaa
    16501 aaggagtggc tgcttaattg ccaactcctt ttttttataa aaagattttc tgtttaaaga
    16561 aggatatttt tttctagttt tttcgaaaaa aaggcgcttt ctgattgttg tattagtgga
    16621 aaggggaaaa aaggagcata tttatcatgc aacaaaatta caatgagaaa tctatacaac
    16681 atcaatttga tgcctactgt aaaaaagtat tacgaaacga aactaagtct attcgaaaac
    16741 gaaacattaa cataacagat aatgaggaac cattgacgaa ttcaaatgaa ggtaagtact
    16801 cccaaactca ttttgatgaa caagatgtct atttcttatt tggaatggaa atattaattt
    16861 cagatcaaaa gttatcggca gccattgatc atttatcgga aataagaaga aaggttatcc
    16921 tactttatta ttttgcaggt tttaatgata ctgaaatcgg taaaatcctc aatatgagta
    16981 caagtggtat ctggtatcaa cgaaagaagg cagttgaaca attaaaaatg gggtatgact
    17041 tatggtagac aaccccaaaa aacggcagta tccattagtt cctctatcca ttatccaatc
    17101 tgctctagaa gaagatccaa tttcaatcaa gatgatactc aaacattatc atagttatat
    17161 agctaagtta tgtttgacaa atggatttaa tgaggcgggg caatttatta cctatgtaga
    17221 tgagtatatg ctaagacagc tagaaattaa attgatagaa gcgatcttaa aatttaaaat
    17281 taactaacta aaaagtcccc tttccactta ttctgtttaa aagaatcttc taatagttaa
    17341 aggattagta gattctttta aacagaaaac ctattgatca ttgaaattta aatacattct
    17401 atcagtacgt taacacttca aggagcatat tttagttgcg ccatgatttc ttactgtaaa
    17461 gaaggagcga taaacattct aaaataattt ttatagtcaa taaaaagcga tgatttgaag
    17521 ttgggataat gatacttccg taaggctcgc cgtaaacttg gagagaggtg aaattcctat
    17581 gtgcttttga caaaagcagc tgattaagaa ttatttccat atgttgattt caacattttt
    17641 tctattattt ttaagaccaa attcagtgag gcaaatagca tcatgtaaat ttagtttttc
    17701 tattgaacgt tctccacttt ttagtttttt tatagtactc aaatttaatc ctgtatgttt
    17761 agagatctca tttgcagtat ggttttgtaa agtttctcgg attattgtta tattagcaat
    17821 ttcattacgc ataattatgt tcctttctta tcaaaaatat caatgactac agtgggcttt
    17881 tagtgaaaaa aaggggtact acagtctctt ttgaaaacaa aaaaatacag tatatacgtt
    17941 ttctaaatgc taagcaccaa cgtttacact gtatttaagg gtttggcacc caatgggctg
    18001 tgaggggctc gaacccgcga cccgatgatt aagagtcatc tgctctacca actgagctaa
    18061 cagcccaaat taatactttg ttattctaac acctaaaata aaaaagtgca atacttatta
    18121 tcagaattga gagtgaattt tatggatttt caagattatg aaaaaatatt tgaaagttat
    18181 ccatcgatat tgaaaattac tgatttaact aaaatgttaa atattggaaa aaataaagct
    18241 tataatttgc tgaaagatag atcaatagaa aatataaaaa ttgga
//
LOCUS       DANKAZ010000051        16527 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000051 DANKAZ010000000
VERSION     DANKAZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(68..343)
                     /locus_tag="PWY67_001692"
     CDS             complement(68..343)
                     /locus_tag="PWY67_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL2057962.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(586..1896)
                     /gene="der"
                     /locus_tag="PWY67_001693"
     CDS             complement(586..1896)
                     /gene="der"
                     /locus_tag="PWY67_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL2057963.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2078..3307)
                     /gene="rpsA"
                     /locus_tag="PWY67_001694"
     CDS             complement(2078..3307)
                     /gene="rpsA"
                     /locus_tag="PWY67_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL2057964.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3442..4122)
                     /locus_tag="PWY67_001695"
     CDS             complement(3442..4122)
                     /locus_tag="PWY67_001695"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL2057965.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4171..4806)
                     /locus_tag="PWY67_001696"
     CDS             complement(4171..4806)
                     /locus_tag="PWY67_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2057966.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4872..6275)
                     /locus_tag="PWY67_001697"
     CDS             complement(4872..6275)
                     /locus_tag="PWY67_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL2057967.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6262..7293)
                     /locus_tag="PWY67_001698"
     CDS             complement(6262..7293)
                     /locus_tag="PWY67_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2057968.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7324..7545
                     /locus_tag="PWY67_001699"
     CDS             7324..7545
                     /locus_tag="PWY67_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL2057969.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7710..8447)
                     /locus_tag="PWY67_001700"
     CDS             complement(7710..8447)
                     /locus_tag="PWY67_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL2057970.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8600..9940)
                     /locus_tag="PWY67_001701"
     CDS             complement(8600..9940)
                     /locus_tag="PWY67_001701"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL2057971.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10177..11700)
                     /locus_tag="PWY67_001702"
     CDS             complement(10177..11700)
                     /locus_tag="PWY67_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL2057972.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12078..12689)
                     /locus_tag="PWY67_001703"
     CDS             complement(12078..12689)
                     /locus_tag="PWY67_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2057973.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12812..12937)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13019..13735)
                     /locus_tag="PWY67_001704"
     CDS             complement(13019..13735)
                     /locus_tag="PWY67_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2057974.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13741..14310)
                     /gene="scpB"
                     /locus_tag="PWY67_001705"
     CDS             complement(13741..14310)
                     /gene="scpB"
                     /locus_tag="PWY67_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL2057975.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14294..15091)
                     /locus_tag="PWY67_001706"
     CDS             complement(14294..15091)
                     /locus_tag="PWY67_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL2057976.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15061..15432)
                     /locus_tag="PWY67_001707"
     CDS             complement(15061..15432)
                     /locus_tag="PWY67_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2057977.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15451..16338)
                     /gene="xerD"
                     /locus_tag="PWY67_001708"
     CDS             complement(15451..16338)
                     /gene="xerD"
                     /locus_tag="PWY67_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL2057978.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4642 a         3577 c         2800 g         5508 t
ORIGIN      
        1 ttttctgcta ggaaataaaa agaaaagcac ttgtagccct agagccacaa gcgctttttc
       61 aactgtctta tttaacagcg tcttttaatg ctttacctgg tttgaatgca ggtactttgc
      121 tagctggaat ttcgatttct tcaccagttt gtgggttacg acctttgcga gctgcgcgtt
      181 cgcgaacttc aaagttacca aaaccgatta attgaacttt ttcgccgtta gctaaagcat
      241 cttgaatagt tgaaaataca gcatctactg ctgcagttgc atctttttta gttaaatcag
      301 tagctgaagc aactttttcg attaattctg ctttgtttgc catggataga ttcacctcct
      361 ccacaagaga tatgatagaa atatcattta gttaaaactt tctgagtggt ccagaagttt
      421 aaaaagaaat attgattaac ttcaatattc tgttatcacg atatcatatg aaccgcttaa
      481 tagcaaggtt tttaccaatt ttcacatgct ttttttagtc atagaaacgt aacaaacgaa
      541 actgtctttt tcttttgaaa aaaaaacgtc gatatggtaa aatgcctatt ttctcctacg
      601 agggataatt cggatggacg tcccttcaaa agtaaatgct tttctgattt gattttctaa
      661 gaaacgttca taagaaaaat gcatcaattc ctcttcattg acaaaaacga caaatgtagg
      721 tggtttgaca gcgacttgtg tagcataaaa aatcttcagg cgttttcctt tatctgttgg
      781 tgttggattg attgctacag catccataat gatatcattc aacacagccg aagagatacg
      841 aagattctgg ttcatactta cttgttcgat catttcaggc agacgttcca accgttgttt
      901 agtcactgca gaaacaaaaa cgattggagc gtaatcaaga taccggaatt catcacgaat
      961 ctcttgttcg aaatcacgca ttgtattggt ttctttcttt acaagatccc atttattcac
     1021 gacgataata attccacgac cagcttcatg ggcgtaaccc gctactcgct tgtcttgttc
     1081 ccgaatccct tcttcagcat tcagtaccat caaaacgata tcagaacggt caatggcacg
     1141 cattgcacgc atcacgctgt atttttcagt attttcgtat acttttccgc gtttacgcat
     1201 accagctgta tctatcatta agaatttctg accactttca gaaacgaagt gggtgtcgat
     1261 tgcgtctcgg gtcgttcctt caatatccga aacaattaca cgttcttctc ctaaaatcgc
     1321 attgatcaaa gaggatttac caacatttgg gcgaccaatc aaactaaact taatcgtgtc
     1381 gtcgtcctct tcttcagtct cttcagagaa atacttcact gcttcatcta aaatatcacc
     1441 aatccctagt ccatggcttc ctgaaacagg ataaggatcg cctagcccta aggaataaaa
     1501 ttcatagata tcgtttctca tttctggatt atctacttta ttgactgcta aaatcactgg
     1561 tttattactt ctgtataaaa ttttcgcaac catctcatct gcgtctgtca cgccttcgcg
     1621 tccgctgact acacaaatga ttacatctgc ttcttcaatc gcgatttctg cttgatgctt
     1681 gatctgatcc ataaaaggtt catcgccaag atcaattccg cctgtgtcga tcacgctgaa
     1741 ttctcgacct aaccattcac cttttgcata aatacgatca cgagttactc caggagtatc
     1801 ttcgacaatc gagatacgct caccagcgat gcggttaaaa atcgtagact taccgacgtt
     1861 cgggcggcca acgattgcaa ttgttggatt tgccatatta tttcctcctc acaagtttcc
     1921 aatctcactt agtttaacga atgcattgcc aactttcaag catagattgt caattaaacg
     1981 aagtattttt attccttatt gaaatactaa tcgcttttca aaagaaaaac tgagacaaaa
     2041 gcagctgctt tcatctcagt cacagataca aaatcaatta tttttcagaa tcatcggttg
     2101 ctttttcttc tacttcttct ttcaacgcat cacctaaaat gtctcccata gtaaatccag
     2161 tattttcttc tggtaattca taagactcaa cattttttgg ttcttcttct gaagctggtt
     2221 tttcttctaa agctttgata ctcaatgcaa tacgatgttc ttctggatgg atctctaata
     2281 ctttcacttt cacttcgtca ccttcatgaa gcacttcatg tggtgtcgca atatgtttat
     2341 gtgaaatttg agagatatgg actagccctt ctactccagg gaacacttca acaaaggcac
     2401 cgaagcttgt caaacgttta actgttccgt ctaatactgt gccaactgga gcttttgtat
     2461 cgatttctga ccaaggtcca ggctgtgtgt ctttgattga caaggaaaca cgttcttgtt
     2521 caggattgat agacaacact ttgacattta catcatcatt gacagccaat acatcacttg
     2581 gttttgctac atgactatgt gaaatctctg agacatggac tagaccgtca attccgccta
     2641 ggtcgacaaa tgcgccaaaa tcagtaagac gtgcaacacg tccactgaga acatctccct
     2701 catggatagt tgaaagaatt tcttttttct tttcttctct ttcgttttcc actaccgctt
     2761 tatgcgataa gatcaaacga ttttctgatg gttcgatttc gatgatttta aagctcaatg
     2821 ttttgccttt atagtctgaa aagtctgcaa caaaatgatc ttccaccatt gatgcaggga
     2881 caaaaccacg tacaccgacg tcaacgacta aaccgccttt gacaacattt gtaacaggtg
     2941 cttcgatgac cttgccctct tggaaatctc tttcgatatc ttcccaaact tttttagcat
     3001 ctaaacggcg ttttgaaagt aggtagctgc cgttttcttt atctttgccg atcgtactga
     3061 taacgactaa atctaataca tcaccgactt ttactacttc gctgatatct tctaccggca
     3121 aagttgataa ttcttttgcc ggaacgactc cttcaactcc agccccttcg attccgacaa
     3181 tcacttgttt gtcttcaatg actaaaactt cgcccttaac aacatcgcca acttttactt
     3241 cttgtacgct tttcattgcg tcttccattg actggttgtt ctcttgattt tgatcaaatt
     3301 ctgtcataac acttgtcctc ctaaccaaca ttctgccgta agtacggtac tgtttttagt
     3361 ttaattgatt attcaaaaaa aataaagcga tttcgttcgt tttccttgaa aaaacaattg
     3421 tttttcattt tttttcttaa tttaaaaaag aagataacct tttttcaaaa caacagcttt
     3481 tattgctgca acgacttctg gaatcgattt gccagtcgta tcgatacgaa cagcatcctc
     3541 tgcttgcttc agcggagaga cgtcccttgt tgaatcataa tggtctcttt cctgtatctc
     3601 tttcgtcagt gtttcaagat cagtcggaat tcctttttct tggttctctt tgaaacgtct
     3661 ttctgcacgc tcacttacgc ttgctactaa aaagattttc acttctgcat tcggtaatac
     3721 agctgtcccg atgtctcttc cgtccatgac aacgccaccg cgttccccta tttttcgttg
     3781 gacagcaact aattcttctc tgacttttcc atgtgcagag acttcagaaa cagcttttgt
     3841 gacatctggt tgacggattt ctaaagtcac atcttttgat gctatgaata catgctgtcc
     3901 atcttcttgt tgcgaaaaag agataggata ctttttgatc agcgaaacta gcccttcttc
     3961 atctgaaaaa gatacattgt tcgtaatagc taaataagtt actgcgcgat acatggcacc
     4021 ggtatctgta taaatataat tcaattcttt tgctaatatc ttggcaacgg tacttttccc
     4081 agatgaagca ggcccgtcga ttgcaatact gatttgtgtc atgatataac tcctttttct
     4141 cagaaaaaaa gccgcaaagc gcggctatta ttatttaatt cttaattctt gaccagggta
     4201 aagcataaag ttgtttggat ctaagccatt caattcaaac aattgatcag tggtgatccc
     4261 tgcacgctca gccacttgtt ttggaccttc gcctgcttga acagtcgttg ttgaaccggc
     4321 tgcagctgat gaagaaggtg ttgtttctgc tgcattgctg cttgaatccc ctgcttgata
     4381 agttgcctca cttgaggaag tcggtgttgt ctctgctgca atactgcttt ctgacgttac
     4441 acctgtatct tgagacacag tagattcttc cgcgattgta ctagattcta ctgttgaagt
     4501 ctcagttgac tcctcttttg ttgtacttgt agaactttct actactgatg tcgaagtttc
     4561 agaagaagtt gctgctgttg cattgttgct tgatccattt ctgatccaga aataagcacc
     4621 tgctggaata gcgatacata agaccagtaa aatcaaaaat agcgaaagaa ataatgtatt
     4681 tcctttcttt tgttgtctct gagaacttct tgatgaaact tcatcatctg tatcgtagat
     4741 tggttgttcc catggttctt gtgtttcgtt attttctgta gattgatgtc tatcttttct
     4801 gctcactgtt ttacctccta aactctatcg tgtgtattat accataggaa aaagtgtctt
     4861 gtcctaaaaa atcaatgttc catgtttttt ttaaataaat ttagtaaaat cgtttcccaa
     4921 tgaagttgtt cagtcttaga aaaaagcttc ctctttggca gaagttctgc ttttgctcca
     4981 tcaatatcac aacaattctg cggcttttcg gagagttttt cgccaaaata agctaagatg
     5041 aattttctgc ggcagttttc ttcattaata tagcgaagca tctgctgcag gcggaattct
     5101 ttttcttttt cctgacgttt gagccgttcc acccaatttt ctggtttttc agactgctgg
     5161 atcagctcca tccatttttt ttgcagctca tcaaagggag cttgctttgt ggcatattcc
     5221 aaataaagct caaaactttg tcgctgttct ctagaaagtt gattaaagaa ataatggata
     5281 cgttcatccc cactttgata aaaaagaatt gctgcacttt cttcctgatc tctgcctgct
     5341 cgaccgattt cttgtacata gttttctaga ctgtctggca aatcataatg gaccacataa
     5401 cggatatcag atttatcgat ccccatacca aatgcattcg tagcaatgag aaactgcagc
     5461 tgatttttta caaactgctg ttgaagttgt cttctttgag ctgcatcaag accaccgtga
     5521 tagtatccaa ctgtaaaacg ttccctgaat aaatgataca atcgttcgac ttcttttttt
     5581 gttgcacaat aaatgattgc cgctccttgt gctctttcca tgaagtgttc aagttcttgt
     5641 tctttttgct gcgttttccg aacaaaaagc gagatatttt gccgattgac tgattgtctg
     5701 atctcttttg gtggtcgatc aaatagtaca tgacgtatat ctcttgctac taaatctgtt
     5761 gctgtcgccg ttagtgccag tgtgaccggg tttcctaaaa gctttcggat ttttcctaac
     5821 tgttgatatt ctggacggaa atcaactccc cattgagaca cgcaatgagc ctcatctact
     5881 acatataacg caatattttg ttttttcaga tgctctaaaa gagaaggatt agtcaacatt
     5941 tcgggactca aaaacaaaaa tttatattgc gatagatgac ggaggacgta tcttctttca
     6001 gattgcgaaa gtaaactatt gaaagcagct actcgtttct cccctcgttt ttgtaattgc
     6061 gtcacttgat cttccattag agaaagcagc ggtgacacga tgatgaccag accttccatt
     6121 aaataccccg tcaattgata gcaaaggctt tttcccgttc ctgtaggcaa aatagcgaga
     6181 gtatcttctc catcaagcaa cgcctgaata atttcttctt ggccgggacg aaatgaatca
     6241 aatccaaaat atttttttaa ttcatttatt aacattctgt atcgcttcct ttctcaatag
     6301 atagatttga tacaaacgaa attctccata atctaattgt ccatctacat tccattcgct
     6361 aaagcgccac tctcgcacgg aatccttatt tggttccaaa cgttcgattg tttctgcaga
     6421 gagaatccac tcgtatggaa aatcatcaaa aaacaaggcc cactcaataa gatgatctgt
     6481 tactgttcct ctttttaaat gacggattgc catgatctgg tcaattgtct gaccattcat
     6541 aaacatctgc ttggtgatca tcatgctttg attgaaattt tgctgtaata atgaatcgat
     6601 caaagcgtgc caaagcgaat ctggccgttt ttcgatttgt gctaacaaaa ggtggataca
     6661 tctgctttgt tgcagataaa cctctgactc atcattgttt tttgccagct gataaggaag
     6721 cagccctgtt cgctcatttc cagataattg gttagctaaa aaatctgctg cttcttctgg
     6781 tatctccgaa aacagctgca ccaaactctc atacgcagta tcaatcaaaa tttcacgtgg
     6841 caattggctt tcactaagcc atttttttag ttggaaagta taaaaaggac ttgtctctgc
     6901 tggaagatag tcttgaatac ccgttgccag attagaaatt acttgaacag caaatttaat
     6961 gagccgccaa ctagtttccc ctgttctgcc atagcggtca aaacgcaaac cagtatgttc
     7021 tatttgatgt tcggacaaca tatcagcccc tgcaggcgtt agctttgctt cattttcatt
     7081 tatttcgatc caaccttgct gcgcaatttt ttgcaaaatt tgataaaact tatcctgctt
     7141 caattctgga aaactgttat ggataaacaa aagttcgtgg gtaaatccat aaataagtac
     7201 tgaagtcgtt cgttttccca caaggagttg gtaaagagaa ctaatccgca gcttgtgtcc
     7261 tgatgaaaat agcgataaaa taaaaacaat catattattc tccttaagtt aggatggtta
     7321 gaaatgtcac tactatgtaa acttgtacct gaacgctgta ttgcatgcgg tctttgtcaa
     7381 atcgaagcgc cagatatttt cgattatgat gaagaaggaa tcgttttatt tgcccaagaa
     7441 ccagaggctc atcaacagtt cgtgatgccg gaagaagaaa catatgtaaa aaaagcgtat
     7501 caaaaatgtc cagttcgagc gattttatta gaaaataaaa tataaatata aacgaaactt
     7561 taaaaacgta cactaagttc tctaaacttg aaaataagcc gggatctgtt tctcttgttc
     7621 cgtgaaatcc cgacttattt tgaaagattc atttgctatc tatagttaat tgactagttt
     7681 tttcacggac cctataggac ccatttatat tatttcaact ttttcagcca gttttccaat
     7741 gtattgacga tcacagtttg ctggtctgcg ttagaaattt ctgcctctcc atctcctttt
     7801 tgctcaccat agctgccaaa acctccatga tttccgccat tgattgatac atactctgtt
     7861 ctttccggta aatactgctt agcttcttga taagcttcct gattcaatac tttatctttt
     7921 gtcgccgtga tcgataaaac aggaacagct gattctgtca atgttccttt tttgtcggga
     7981 taacttgcca tgaagaatac cccttttaaa tgtttgtctg aatgattctt attgacataa
     8041 cgacttgcca ttacgccacc taatgaatgg ccaccgatca cataatcgga atttggaaaa
     8101 tcttctttga ccttatccgc tttgtttccg cccatcactg ctagatcaag tggcattttg
     8161 acaatagcga ctgaaaagcc gttttttgct aaatcttgcg cccaaatact ataactttct
     8221 ggctctacga gagcaccagg ataaaaaata agtaatggag actttccatg attctttttc
     8281 tctccttcaa aaaataagta atcttctttg ttttttcctt cttttaccaa cacttgcgct
     8341 tctgacgatg gttcgtacgt attttgctgt atatagtaat aaccggctcc accgagtaat
     8401 cctaaaatga tgactaagcc aattagtatt cgtctaaata ttttcacttt ctcccacctt
     8461 ttctatctga gtgacccctt tttcttaatc aagtatgatt ataccttaaa aaaataagag
     8521 agaaatgcaa actttccagc atcaaataaa aaaagacgga atatccattt gaatatccgc
     8581 cctttttgta tgtttatttt tatttagcac ataaacgata cacagcttct gcataaattt
     8641 ccattgcttt atacatacta cttaattccc atcgttcatt cgcctggtgc ataaagtcag
     8701 gagtatcagg gaacatcgca ccgaatgcca cacactggtt catcgtacga gcaaaagtag
     8761 cgccgcctga tacaaacggt tcagtcatgt ctccagtaat atcgcgatat gtccccaata
     8821 gatttttcac taactcactg tctagtggca cgtataatga atcaaggaag tcaaattctt
     8881 cataagtcaa cccatatttt tctgctgttt tagttaattt agctactaaa tcatctttct
     8941 tgaatgtaac agggattctc atatctacac cgatttttgt ttgttctggt gtgatttcca
     9001 aacttgcaaa gttcatcgtt agctcgccgg attgttcatc ttctgttttg cccacgacag
     9061 cttcaccagt tgcattttct tgaactaatt ttcctaagaa attgattggg ccaaaatcaa
     9121 atacttctga aagagcaatt gccagacgag aaatagcatt cactccttgt gctgcatctt
     9181 tcgcgtggat acttttacca agaacgacga tgccttcacc ttgttcttcg aaatcgaatt
     9241 cgtgtttttt caatgcttct tttacttcag aaagcttttc tccagagtaa ggagctgcat
     9301 caggaacaac attcaacgct cctccagctt ttacagaaaa ctcgttcgtt cctggtcctg
     9361 tcaagtaagc ttgcaataag cctttttctg cataaattaa aggaaattca gcatctggcg
     9421 caaatccttg cgtaattcct tcttcttttt cgttatattt ttctaaacaa cgccataaat
     9481 tttcttcatc tgtaccaaag ataaaacgaa tacgtgtctt gaattccacg ccagcatcaa
     9541 tcaaagcttt gacggcatac attgccgcaa tagaaggacc tttgtcatct tgggatccgc
     9601 gaccaactaa ccagccgtct tttatagttg gatcgaatgg ttttgtttcc caattatttt
     9661 cgtcccctgc tggaacaacg tccatatgac acaagatacc gaataattca tctccagagc
     9721 caatctctga ataaccgtaa tagccttctg gatctttaaa tgttctaaat cctagctttt
     9781 cactgatttc caatacttta tccaaagctt caatcacttt tttaccgaag ggatgtcctt
     9841 gtcctgaatc tgcttcatct agtacggaag gaattcggat caattcgctc aatgattcta
     9901 atgcttgttc atgatgttgt tctgtcacaa attttttcat gatattatcc tcctaatatg
     9961 ttaaaaaggg tcatgtcaaa ttcctccgac acaacctctt cttctcaaat aatgacgttt
    10021 taagcctgct ccatcagcaa gcagaatgtt cttaacaaat gatgaaagaa aaaacactca
    10081 ctgaatggag ctattgactt gtccacgtgc ttatcgatag ccgtttatcg gtttcttcat
    10141 tcttatgata gaccttgtac ctgtatttgt ctaggtttag aacattgctg ctacacctag
    10201 gatcacacag atggctacaa ataatatcgc aattaattta ccggtaaatt tccaccacac
    10261 tgaaacatcg aaacgagcaa ttgctacagc acccattacg atagcagatg ttggtgtaat
    10321 taagtttact aaaccagatg cagattgata agcagtgatt actaaatcct taccgacgcc
    10381 ggcaaattca cccataggtc ccatgattcc cattgttgca gcagctaagc cggaagttga
    10441 aggaatcaag aatgacatag ggatatagaa aataaatgta ataatgatga atacacttga
    10501 agataatgaa cttaatcctt gttcgcccca atgtaagatg gtgtctgtga ttaatccatt
    10561 gttcataacc acttggatac cacgagcaac agctactacg attgctacac cgatcaaatc
    10621 agaagcacca gctaaaaatt cagagacaaa ttcagactct ttcatgccat aagtgattgc
    10681 tactacgatg gacattacta agaataacat agtaatttct gggaagtacc agtcacctaa
    10741 tggaagcatg cttttaccta gaaccgtacc tagtaccgga aggtttgtta accagtttgt
    10801 caatgattca aaaatcgtga aattcgcatt caaggttgtc caaggaatca aactgatgat
    10861 catgatacca aaagtaataa agaacaatac attaacatgt ttttgttttt tcgtgattac
    10921 ttcttgacga ccgattgctt caatatcgaa atgcttcata tcttctttac gttggttgta
    10981 tactaatgat tttgttggat ctttttcgat ctttgatgca taacgataca catatacaat
    11041 agaaatagct aacaaaataa cgaacatcaa gaatcgaaga ccaatacctt cacccatcga
    11101 aatcccaaca gcttgtgaag ctacgcctgt tgcaaaagga tttaccgttg aagctagaca
    11161 gcctacttga gaccctacta atacgattgc tacagctact actgtatcaa agcctacagc
    11221 catcatgact gggatcagta aagcatagaa tggaatcgtt tcttctgcca ttccgtaagt
    11281 agagccgcct aatgcaaata acagcatcaa tactggaata agcatttttt cttttccttt
    11341 gaatttcttc acaactgaag caatcccggc atctagtgcc cctgttttgt tgataatacc
    11401 taaaaatcca ccaacaacca aaatgaagaa agagattggg attgcagcag gtgtttcttt
    11461 agttagctcc cccgctggta cacctagcat cccattgatt ggcgccatga aaatatccca
    11521 cagcccttga ggattttgtt gtactcgttc atatgatcca gcgacgatgt tgcctgcatc
    11581 gtctacacta tagtgcccag ctggtataaa ccaagtaagt acagcgatta ctgcaataat
    11641 aataaataag accgtatacg ctgaaggcat ttgaaatttt ttcttttttg tttgttccat
    11701 cttcttcttc ctttcttttc cgttagagac aacaattttg gctctaatgt tattttatca
    11761 aaaagagtat cagaacaaaa ataataacac gcattttgta aaacttttgt tcaaaaagtt
    11821 cgtttttccc tattccctcc tcttttttca gttttttaaa gctttcttct ttttcattgt
    11881 agcgtttcct tttttttata aacaaacatt ttcgtctttt aaaaggagaa gaatttcaca
    11941 tttgcaaaga attgattgat ttttatgcat aaatcaaaaa aatactcaaa acaaacaaat
    12001 aaaacgttta cattattgca tgtcttgtat ttttttccaa aaaaaaagac caagaaattt
    12061 cttgatcttt ttgatattta tttgactgct gtatgacgtt gttcgaactg atgttgtttt
    12121 cgacttaacc atggaagaag ttgcgcatgc agcactaaaa agacagcact cacgatgaat
    12181 cctttgatca agttgaaagg aacgatccca atcagtacat agtttgctaa agacatgccc
    12241 agcatctgtg ttgcgttcag ccctaaaaac attaagtaaa gaggcgtaat aacgaaataa
    12301 tttgcgatac tcataaaaat agttagagcg atggttcctg taatgacacc taaaaatttg
    12361 tttcttcctt tatgctgttt ctttacacta aagaaataat aaatcgggaa tgtaaagatc
    12421 gttgttgata agaaactagc agtgtctcct accaaattat ccggagaaaa acctgtagtc
    12481 agcagatgta atactgaacg caagaacgct gttgcaactc cggccgctgg accgaataag
    12541 aacatgctga tcaataccga gatatcacta aaatcgatct tcaaaaaact aaaagccggc
    12601 atgatcggaa aagcaaaata ctgtaaaact aatgcgatcg cagcaaacat cgctactgca
    12661 accattttct gtacacgggt gttcttcatt tgaaatcctc ctgttaggac tcctcttcag
    12721 cgaacttttt gacgtttctc tccaaatgaa aaaactctat tttccaggga aaatagagtt
    12781 agagttattt gatcaagcgt cgtcaaataa gctctatctt ctttatccag actatactgt
    12841 cggtatcgga atttcaccga ttcagctatt tttttgcaaa ataggtcgtg gactataacc
    12901 accggtcggg aatttcaccc tgcccctgaa gacgaacctt atataatttt tgtttacata
    12961 ttcattatac atatctattc aaaaaacgca acatctaaca attataaaaa gtaagtgttc
    13021 attttgagtg gttcagcaaa ctgctgactt cttttttgtt cagttcacgg taatccccag
    13081 gacgtaggcc ttgtaaagtc agatctccgt atttttcacg ttttagtttt tgcacaggaa
    13141 gaccaactgc ttgaagcata tttttcactt gatgattacg tccttcgtgg atcgtcaatt
    13201 ccacgatact gtggtttgtc ttaggatcca ctgataaaat ttggaattta gctggagatg
    13261 tttttcgtcc ttcgatcttt attccttttg tcaaaggtgc aagcatcgat tttgtagcga
    13321 tcccttttac ctttgcaacg taaactttat ccacttcatg ttttggatgt gtcaggcgct
    13381 gagcaaaatc tccatcattt gtcagtagca agatccccga agtatcgtaa tccagtcgac
    13441 ctacaggata gatacgttct tttaccatcg gcaaaagatc cgtaacaact tttcttcctt
    13501 tgtcatctgc aacagcagaa ataactcctt taggtttgta caataaatag taaccgtatt
    13561 cttcttgata aattgggact tcatccactt ccacagtgtc atgtttccca acttttatcc
    13621 ctaattccct tacaacttgt ccgtttactt ttacacgtcc tgcaaggatg aattcttctg
    13681 cttttcgacg agaagtgatg ccagcgtggg caattacttt ctgcaatctc tccattacaa
    13741 ttattctcct ttattcattc catcaaagaa tagatctggt gacacgtctt cttcaagtga
    13801 aatttccatt tcttgtgtat caggcagttc ttctaatgat ttcaaaccaa aataatctaa
    13861 gaagtagcca gttgttccat ataatatcgg ccgacctggt ccttctactc tacctttttc
    13921 ttcaatcagt ttatgggcag ctagacgctg taccgctcct gaaacctgaa ctcctcgaat
    13981 ctgctcgatt tcagctcttg tgattggctg tttataagcg atgattgcta aggtttcgac
    14041 ggcggcttga gataatgtgt ttgatgtccc ttgcgcatat cgcttaagta agggagccag
    14101 ctctttttta gtcgttaata cataatggct ttccgtttca aaaatatgca aagctgagtg
    14161 cgagttttga gtgtaagatt cttttaattc tttcactaaa cttgtgactt ctgcttcgct
    14221 gagatttaac agtccgctta attcttcatt actgatccct tcctcgccaa caataaaaag
    14281 aagggcttca agttcactta atttcgtcat gttcggtatc tcctactgct ggataaagca
    14341 taattgattc atagttttct ttttgttgcg caatgatctg cccttttttc atcaattcta
    14401 gcaaagccat aaaagtagtg acaacttcac ttttagaagg tgactcaaaa aaagaatcga
    14461 aagtacagcc atgcgcattt ctttttatac gctcttcgat aaataccatt cgttcttcga
    14521 tcgttgtgtc atcgggcgtg atcgtttttt ccactttttg agaaaatttc ttacgttcta
    14581 agattttctg catggcacgg atcaaatcga tttttttgac ttgtcctcgc ttcaacggct
    14641 tttcagcatc cgctaaccca tcgatgttca taggttcttt tgtgtaataa agacttcgtt
    14701 cctctgcttt ttctgtcagt ctttcagctg catatttata tttacggtat tctaaaagct
    14761 gagtgaccaa ttcttctcga ggatcttctt cccataattc ttcatcgtct gtgccgaatt
    14821 cttggacagg aagcaatgtc ttgcttttga ttgccatcaa tgtagcagcc atcactaagt
    14881 attctcctgc aacagctagc tcgagtgttt tcattgtatg gatataattc atatattgct
    14941 ccgtgatcgt agcgatcggt atatcatata tatcgatttc catttgctga atcagatgaa
    15001 gcagcagatc gagaggaccc tcgaatacat ctaatttaat attgatctcc gtcaactcat
    15061 tcactctttc ttctttttct ttgctagcca ttttccaatg taaggctctg tgatttcagt
    15121 tgcacggatg gagacatccg ggtacttttc ctgtaattca tctaacattt gaaagcccat
    15181 attctcaccg cgataagacg gattcaaaga aatgtcatgg atgatcagct gatcgttcgt
    15241 tgcccagtaa ataccaataa tcccttgaat gttggttgaa ccttctggcg taaagaaaaa
    15301 aagttcataa tcgtcttcag attcgtacgt atcaatctcc ttcaacaaag aagttttacc
    15361 tgtcagtttc ttatggaaac tcaatagtcc catagcaatt ttacggtttt ctttccggta
    15421 atgtgttaac atcttctcac cttcctatga ctaagccctt ggaaaatgct gcttataaac
    15481 atccgtcatt cttttcttcg tgatatgtgt ataaatctga gtcgtagaga tatccgcgtg
    15541 acctaacaat tcctgtactg tccgcaaatc agctccattt tctagcagat gtgtggcaaa
    15601 gctatgccgc agcgtatgtg gtgttacttc tttgtaaatc ccagcctctc gtactaactg
    15661 cttcagattt ttccagattc cttgccgaga aagacctttc ccatgattat tgacaaatac
    15721 atgcatttca gatgcgtcct ttacaagtaa tggacgtgct tcgtctaagt agcgttccag
    15781 ccattggatc gcataatcac ctaaaggaat gatccgctct ttatctcctt ttccaactgt
    15841 ctgtaccagt ccaagagaaa gatgaagatc tcccaatttc aatccaatca attcactgac
    15901 gcgcatccca gtagcataca tgacttccaa gattgcacga tctcttatcc ctaacgtttt
    15961 tgtcgtatca ggtgcttcaa tcagcttttc tacttctgtc agagtcaatg tacttggtaa
    16021 tttctgtgct ttcttcggcg tatcgatatg ttgcatcgga tcatgatcag tgatacgctc
    16081 ttgccgtaaa aactgatgga atcttctgag acttgagatc atccgagtaa cagttgctgt
    16141 cgcttttttt tcttcatgca acttctccag aaaacggatg actaaaaagc gatcgacatc
    16201 ctgccatgaa gcaattttct gctcctgtag aaaatgcaag tactgttcca aatcacgttc
    16261 atagctttgt cgtgtattgg cggacaatcc ccgttcaata ttcaagtagt ggagataatc
    16321 gttgatttgc tctttcataa taatccccct catgcctctc tatcatatca gataccgctt
    16381 cattctgata gtctttttcc ataatctgag caaagaaaaa gccgtctaga cactcagacg
    16441 gactggtttc ttttttcggc tctataattt atgggactga tgaatcagaa ttgattcttc
    16501 agccccattt tcattttagg tattaaa
//
LOCUS       DANKAZ010000052        16508 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000052 DANKAZ010000000
VERSION     DANKAZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(271..1206)
                     /gene="murB"
                     /locus_tag="PWY67_001709"
     CDS             complement(271..1206)
                     /gene="murB"
                     /locus_tag="PWY67_001709"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL2057979.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1279..2199)
                     /locus_tag="PWY67_001710"
     CDS             complement(1279..2199)
                     /locus_tag="PWY67_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2057980.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2312..3064
                     /gene="xth"
                     /locus_tag="PWY67_001711"
     CDS             2312..3064
                     /gene="xth"
                     /locus_tag="PWY67_001711"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL2057981.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3928..4461
                     /locus_tag="PWY67_001712"
     CDS             3928..4461
                     /locus_tag="PWY67_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL2057982.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4951..5475)
                     /locus_tag="PWY67_001713"
     CDS             complement(4951..5475)
                     /locus_tag="PWY67_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2057983.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5468..5941)
                     /gene="tsaE"
                     /locus_tag="PWY67_001714"
     CDS             complement(5468..5941)
                     /gene="tsaE"
                     /locus_tag="PWY67_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL2057984.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(6064..7044)
                     /gene="pta"
                     /locus_tag="PWY67_001715"
     CDS             complement(6064..7044)
                     /gene="pta"
                     /locus_tag="PWY67_001715"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL2057985.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7197..7877)
                     /locus_tag="PWY67_001716"
     CDS             complement(7197..7877)
                     /locus_tag="PWY67_001716"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2057986.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            8003..8872
                     /locus_tag="PWY67_001717"
     CDS             8003..8872
                     /locus_tag="PWY67_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2057987.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8898..9413
                     /locus_tag="PWY67_001718"
     CDS             8898..9413
                     /locus_tag="PWY67_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057988.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9460..10053)
                     /locus_tag="PWY67_001719"
     CDS             complement(9460..10053)
                     /locus_tag="PWY67_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2057989.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(10078..10257)
                     /locus_tag="PWY67_001720"
     CDS             complement(10078..10257)
                     /locus_tag="PWY67_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057990.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10334..10843)
                     /locus_tag="PWY67_001721"
     CDS             complement(10334..10843)
                     /locus_tag="PWY67_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057991.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10939..11319)
                     /locus_tag="PWY67_001722"
     CDS             complement(10939..11319)
                     /locus_tag="PWY67_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2057992.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11649..12398
                     /locus_tag="PWY67_001723"
     CDS             11649..12398
                     /locus_tag="PWY67_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL2057993.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12582..13859)
                     /locus_tag="PWY67_001724"
     CDS             complement(12582..13859)
                     /locus_tag="PWY67_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2057994.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13938..14582)
                     /gene="pcp"
                     /locus_tag="PWY67_001725"
     CDS             complement(13938..14582)
                     /gene="pcp"
                     /locus_tag="PWY67_001725"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL2057995.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14781..15725)
                     /locus_tag="PWY67_001726"
     CDS             complement(14781..15725)
                     /locus_tag="PWY67_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL2057996.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15727..16413)
                     /locus_tag="PWY67_001727"
     CDS             complement(15727..16413)
                     /locus_tag="PWY67_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL2057997.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4761 a         3405 c         2907 g         5435 t
ORIGIN      
        1 ccaattactt gatatgcaga tgaaagttca tcttttcacc tatactgatt ttatgatgga
       61 ttatgcttgt tttttcatca gttattctca ttttttatct tttttttgag aatattgcaa
      121 aaaatggaaa aatttatgag gaaatattct atattttttg ttttattgtg ttaaaatgta
      181 caagatttgc gccatttgcg taaacaacca caaaaaagag caggacggcc accgtcctgc
      241 tcttttactt attttaagtt ccattactta ttaattggct aaacttctgc ttgtttctga
      301 tattgcttgt ttttctccaa tgatccgcac ttctgtttct aaagacacat caaatttttc
      361 tttgattact tcttggatgt gcgcaatcaa ttcaatataa tctgtcgctg tcgcatgatc
      421 gatattgacg atgaatccag catgtttttc agagacttgt gcaccgcccc atttcaggcc
      481 ttgcaatcct gcttgttgga tcagctgacc tgtatagtgt cctactgggc gcttaaagac
      541 acttccgcat gaagggtatt ctaatggctg ttttgattga cgcaattcgg tcaactcatc
      601 cattcttgct ttgattgctt cataatcccc tgtttgcaaa ctgaatcgag cttccaatac
      661 gatagcacgt aattcttgga ctttactgtg acgataagaa aataccattt cgtcttttgt
      721 caaagttttc aatgttccat cttcaagcaa aagatccact tcagcaaaaa catctttgat
      781 ctctccgcca tacgcacctg cattcatata aaccgctcca ccgacacttc ccgggatgcc
      841 gcaagcaaat tcaaatccag tcaatgattc tgccaaagct gcataagtcg tatcaatcag
      901 tgaggcacct gcttccgcaa tgaccatcgt tcctttgaca tgaatctgtt tcatttcagt
      961 cagcatgatg actacaccgc gaatcccgcc atcttgaacg atcagattgc ttgcatttcc
     1021 taataccatc caaggtatat catggattcg gcaataatcg atgatttgct ttacttcttc
     1081 ttgttttttc gggaatgcaa ggacatcagc tggtccacct gttttcgtaa acgtatagtt
     1141 catcaatggt tcatcaaaca atatagtaat cgtggctaag ctttcaacta tttcttcttt
     1201 attcactgag gattttcctt tcttttctca aacgtcctca ttattctatc acgcttaata
     1261 caatcttgcc agtcttgttc aaagttgatg acggtagtga tctttaagca cttcctcaca
     1321 taattcatga cttcttacaa cttctttttg tttaagcaat tctgtttccg atttacctaa
     1381 acggatctga gaaaagaagg cctctaccat tggctcaaac ccgcgtttga ctagcgtgtt
     1441 tgtccagtcg ccaaattctt tcactttttt tccatcagcc tcttgaatga caagctgggt
     1501 cagatcgttt acttcataag taccattttt acttgttact tgatattttt ctatattagc
     1561 gccacttgct aaatccatcg ttaaaatagc cgtttgattt tgtgtttcta attgtaaaat
     1621 ggcatattcc aaaaattcct ttgtttctct tatctggtat tttgttctca aaatgggttc
     1681 atctaataaa tacacagctg tatcaataag atgtaaaaac aaatcataaa ggacgaactc
     1741 tgtcgtttct tttgccgcta ttcgattttt ttgcaggtgg atcaaccgct tatctgggat
     1801 acgtttcaat tcttctacca taggagcaaa gcgccggttg aaaccgatca tcaaaattat
     1861 ttggttctta tcagctaatt cttgcaattc acgaacttca tttagatgga cactcagtgg
     1921 tttgtcaata taaacatgga taccattttc tagacatttt ttggctaatt cataatgtac
     1981 ttttgttgct gcgtggatca tacatgcttc gattttttct tctaaaagct catcgatcgt
     2041 ttcatataga tgttggaacc cgtactcttt ttgaattctt tcttttgttt ctttatttct
     2101 tgttgcaaaa taaaaatcaa gtgttccttg ttttttcgca taagttggta aatatgcttt
     2161 ttctgcgata ttccctacac cgattacacc aatcttcatc tactttcctc ctggatcatt
     2221 tagttacttt catcttacac cattttttac taaaatgagg tacaatagta tcgcaaaggt
     2281 aaggaaatta attcgaagga gtgtgctttt attgaaattt atctcttgga acgtaaacgg
     2341 gcttcgtgcc atcgtaaata aaaatttttt agaagttttt catgagttag acgccgattt
     2401 tttctgtctg caggaaacga aactccaagc aggacaaatt gatttagatc ttccaggtta
     2461 ttatcaatat tggaattacg cggaacgaaa aggctattcc ggcacagcga tttttgctaa
     2521 aaaaccagca ttgaatgcca cttacggaat gggtatcgat atccatgata cagaaggccg
     2581 tctgattact ttggaatatt ccgatttctt tcttgtaacc tgttacacgc caaattctca
     2641 aagcgaatta aaacgtttag attaccgatt agaatgggaa gaagcctttt ataattattt
     2701 ggaaaatcta aaaaaacaaa aaccagtcat cgtgtgtggg gatctaaatg tcgctcatca
     2761 aaagattgat ttgaaaaact ggaaaacgaa tcaaaaaaat gctggattca ctccagaaga
     2821 aagagcggct ctttctcgac tgttggataa cgggtttatc gatacattcc gttattttta
     2881 tccaacccag gaaggtgtct actcttggtg gaactaccga ttcaattcaa ggaaaaacaa
     2941 tgctggttgg cggatcgatt actttttgac gagcaaagat ttagagccag gactagctga
     3001 tgcaaagatc catacggaca ttatgggcag tgatcattgt ccagtagaat tagatttgaa
     3061 ataaagaggt tgtgaaaaag tcgtttagtt tcgagtatta agaaaaattt tgtgaaaatg
     3121 gctttccaca ttttgcataa atttggcata ataccgagaa actggctttt gaacactgtt
     3181 tatcgagaga ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
     3241 agttcctcat atttttcgtt tggttgggct tattaccgca gatacaagct tttgaacacc
     3301 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3361 aatagttcct catatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
     3421 accgtttatt aagaggttgt gaaaaagctg cactgcatca agtaataaga acagccaaac
     3481 aaaaatagtt cctcatattt ttcgtttggt tggacttatt accgcagatg caagcttttg
     3541 aacaccgttt attgagaagt tgtgaaatca gcgtcctgac ctgagtatta gagaaacaaa
     3601 tggcaaaata gttcctcata ttttgctatt ttttgagcta ataccgaggg tcagctgatt
     3661 gaacaccgtt tagtggagaa gttgtgaaaa agtcgcctac tttcgagtcg taagaaattt
     3721 ttttgaaaat ggttcctcac aactttgcat aaatttgact taataccgag aagtcatgat
     3781 ccccatctaa ctccaaatca caaggaatga gttcaaaaac tagcttttct caacaaatat
     3841 tacataaaaa attctatgtt ttttgttaaa tttatttgct tttttcaaaa gtatgttaca
     3901 gtaatttccc aaggaggttt tacagaaatg aattttgttg ccatggattt tgaaactgcc
     3961 aatcatcagc cttatagtgc ttgttctctt gcgctagtca tggtaaaaaa tagccagatc
     4021 gttgatgaat tctatacatt gatacagcct gagactcctt ttttctggcg aaatgtccaa
     4081 attcacggaa tccatcaaga agacgtacgc aatgcgccga aattcccaga agtctggcaa
     4141 actatccaac catattttca aaaaaatcgt ttagtcgttg cacataatgc tgcttttgat
     4201 acaaaggtct tagcgggatg cttagattat tatcatcttg aacagcctaa ttatctttct
     4261 ttatgtacag tgaaaagtag ccgtcgcctg tttcctgaat ttccaaatca tcgattgaac
     4321 actgtgtgcg aaaatttgaa catccaatta gatcatcacc atgatgcatt ggaagatagt
     4381 cgtgcatgtg cggaaatcct gctttatcaa gaaaaaaaat tcgggacaga tccattgaaa
     4441 aaacttgtga ctataaagta aaagaatact tatgaaacac acaaagaggt cgtgagcctg
     4501 ccgtttcact ccgagtcaca aggagcaatt tcaaaaaata gcttctcatg tttattaaat
     4561 attgtgactg gtgaccgagg agctggcagg agaacaccgt ttattggaga ggttgtgaaa
     4621 aagctgttct gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttca
     4681 tttggttgga cttattaccg cagatgcaag cttttggaca ccgtttacca agaggttgtg
     4741 aaatcagcgt tttgacctga gtattagaga aacaaacggc aaaatagttc ctcatatttt
     4801 gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttatt caaaaaaaat
     4861 ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt tttctgcctt ctcaaaagga
     4921 tgacagtttc tcactctttt cctatttctt ctattcaatc aaataactca ttttatatac
     4981 atctaaaaat tcacctagat ctgttcttgc ccctctaggc atgaccgctt cgatttggaa
     5041 gcccattttt cggtaaaggt gtacggctct ttcattacga acttgaacgt caagctccaa
     5101 tcgaaaaagt acacccattt ccttcgccca actgatgatt tcttctagca taagagtgcc
     5161 tagtcccatt ccccaatatt cctgcagaat actgataccg acttctccca cgtgagacaa
     5221 acgaaattga ctgtctgcct tgacagatgc tgttccaata atcgtccctt catagattgc
     5281 aagcagcaat aaattattat tgctttcgta tatataatcc aactcttctt ctaaggtttc
     5341 tggtggtaac aggagttctg cttcatccaa aactaaaaat tctgtctcct tattaacttg
     5401 tcccatcatc aaaagaatgt cttttgcatc tcctggcact gcttcccgca agatgatccc
     5461 aacttcttca ctcatgattt tcagcccatt tttctagaat tcgctgtttg aaggtttcac
     5521 tttgctcccc gtatgagatt agtttcacat accgcttttc taaatctgta tcactttttt
     5581 ctaaaatcag ttccaaataa tcctccggca gtgcttcttc taatagattt ccccattcaa
     5641 caacggaaag cccatcacct tcaaaatatt catctaaccc taaatctgct ccgctagctg
     5701 ctacccgata aatatccata tgatacaacg gaatccgacc ttgactatat tctctaataa
     5761 tcgtgtatgt cggacttttg atcatttgcg ttatccctaa tccttgggca attcctttcg
     5821 tcaacgttgt tttacccgca cctagatcac cagtcaatat cagattgtca cctggcattg
     5881 cggaatgacc aattgcctct gcgaattgtt ctgtcattgt taatcctgat agttcgatca
     5941 tttttcatct tctcccgctc ttctttcttt ttgccagtat atcgcgtaat gaagtttttt
     6001 taaagaaaaa atgaactagt caagaacagc agctgctgtt tttggctagt tcgcgtgtat
     6061 ttgttaatca atcaatgttt gtgctgctgt gataatcgat aatttatata catcttcttc
     6121 atttgctcca cgagacaaat cagaaaccgg tttgttcaag ccttgtagaa ttggtccgat
     6181 tgcttcaaag ttaccgaaac gctgagcaat tttatagccg atatttcctg actgcaattc
     6241 tggaaataca aagactgttg cttgaccagc tacttctgaa tctggcgctt tcagttgtgc
     6301 aactgaagga acataagaag catcaaattg caattcgccg tcgatcatgt attgtggcgc
     6361 tagtttttta gcgattttag ttgcttcaac tactttgtct acttctggcg atttcgctga
     6421 gccttttgtc gagaagctca tcattgctac ttttggttcg atatcgaaca attctgctgt
     6481 ttttgcagaa tctacggcga tttcagccaa ttcttgcgca gttggattca cgttgatcgc
     6541 acaatccgaa aagacatatt tttcttgatc tcttccacgt accatgatca tcgctccgct
     6601 tgtccgactg atgccaggct ttgttttgat gatctgaagt gctgggcgaa cagtgtcgcc
     6661 agtcgaatgg atcgctccag agaccattcc gtctgcaatc cccatatacg taagcatcgt
     6721 accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt tctttggatg cctttccatt
     6781 tctacgttca acaaaagtat ccaccatttc atcccactta tcgtaattat cagggctgat
     6841 gatcgtaaag ctagatgttt tgattcctct ggcatgcgct gcatttctta tttcttcttc
     6901 gttccctact aaaatgggtt ccacaagttc ttctgctttc aaccgggcag ctgcgcctaa
     6961 aatacgagca tcagtagctt ccgggaaaac gattttgatc ccacggcgaa caattttaaa
     7021 tcttaaacta tcaaacaatt ccacaagatg acctccagac gtttttatat gtattatcat
     7081 acactattcc agaaaaaaag aacagtttat tttatctgta tcagtcaaaa atgctggaaa
     7141 aattaagttt ttcacataca aagcgaactg cactataaca ggaaagcgct gttttattac
     7201 actgtttcag gcagttgcca atcaatcggt tcttctccta acgcaaggag cgcatcattc
     7261 gttttcgaga agggtttgga accaaaaaag ccacgacttg cagataacgg actgggatgt
     7321 ggagatgtta tgatgatatg gcgactctta tcgatcattt tgattttttc ttgtgccggt
     7381 tttccccaca agataaacac gattggtttt tcccgttcat ttaatttttc aatgatcgtg
     7441 tctgttaatc tttcccaacc tttcccgcga tgagaatacg cttgcccttc ccgaacggta
     7501 agaacggtgt tgagtaaaag gactccctgt ttagcccatg acactagatt tccatgttga
     7561 acaggtgcga ttccaagatc atcataaagc tctttataga tatttcttaa cgatggcggg
     7621 atctttacgc caggttgcac agaaaaagat aagccatgag cttgattttc tccatgataa
     7681 ggatcttgtc ctaaaatcac cactttaact tcttcataag gagtcaattc caatgcttca
     7741 tagatatgat acatatctgg atggatcttt tgggacgcat attctttttt taaaaattct
     7801 cttagatgca gataataatc ttttgaaaat tctgaggaca aaacttcttg ccaactgtta
     7861 tgaatgattt ctttcaaaaa ctccacctcc taataaaaag aataactcac attttcttca
     7921 atcacaaggg acaatcttca ccctcagcac ttgattaata tggtaaacta ggaacaagaa
     7981 gcagaaagga acgataatca ttatgatcaa attaattgca tcagatatgg acggcacatt
     8041 attagatgcc catatgagtg tttctcctga aaatgccgag gcgatacgtt ttgcaaatga
     8101 tgccggtgtt gagttcatgg tcgctactgg tcgaaactat caagaagctc gcgcagcttt
     8161 agatgaggta ggaatcgatt gtgcgatgat cactttgaac ggggcacagg tatttgataa
     8221 agaaggaaat tcattgttta ccgtgtcgat tccaaataat caagcaatct ctgttttaga
     8281 tattctagat gctaacggta tttactatga agtagcaacc aacgatggtc tttattctga
     8341 aaatcaagcg aaacgaattg aaagttttgc ttccatggtg gctacccatt tacctcacct
     8401 tacgtataaa atggcgattg ccatggcctc agctaagctg gagttattgc acatcacata
     8461 cgtggacagt attcgtgcta ttttggaaga aaagaatctg gaagttttaa aaattatttg
     8521 ttttcataca gaagggccag ctgtattagg tccagttgga aagcagattt ctcttttagg
     8581 cgatctagcc gttacttcct ctggtcaaaa caatatcgaa gtgaaccata aaaacgctca
     8641 aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg atttctttag aagaagtcat
     8701 gacgattggc gataacttca atgatgtcag catgctgcaa gtagcaggag tcagtttcgc
     8761 aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa tatacaacgg acacaaacct
     8821 tgaatctggc gtaggaaaag ccatattgcg cgcgattgca gaagacttgt agttttatct
     8881 agaaaagagg gatatagatg tcagaatttt tcattcagga aaagcagctt ggacgtgcaa
     8941 ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt tttgatggtt ggtcgctggg
     9001 gaacaaaggg cgatgcattg tctctttatg ccatgaatgg cgatctcgtt gctcacatca
     9061 agcagataag cctgacattc ggtacgcgtt ttgagctgta taaagagttc gaaaaggtgg
     9121 gaaccatgca gaaaatattc aattggcccg gtgactttta ttatattcgt agacttcact
     9181 ggacagtcca aggtgatatc tataatcatc aatatcaaat ccatcatttc aatcagcaaa
     9241 tcatgaaaat ggataaagca acactttttt caggagatta ctatgtattg gatgtctcaa
     9301 atgatgaaga tgctccagtt tgtatttgta ttgctgcggt tttagattac tggctctacc
     9361 gtaaagacaa aagtcgcgat aagcctgttt cttaccgact taacttaagt taaaaagatg
     9421 tacctgaaat gcggaaatac gcatttcagg tacatctttt tagatattct tttgcatatg
     9481 atcataaaga tgaccactga tcatccgctg accgacaact ttgaagtcat ggcgttcgta
     9541 taatttttta gcctttggat ttttttcatc cacacttaga ccgatgacgg agcgattagc
     9601 tttcttcgcc aatttaggta atgcttctaa taatcgcgaa ccgatgcctt gtcctctaaa
     9661 atcttctgat acactgatag agtccaaata ccattcgttg ggaaacgttt ctttatcaat
     9721 gaacaaacgt gtagacgcat ctagctgata tttctctaag ataggtacca gtggctgatc
     9781 gatgattgat tcttcttcat ctgtataacc gtaagcaact cctgcaactt taccgtctat
     9841 ttcttctacg atacctcgtg aataactgta acgataattt ggatcagtga ttgcttcttc
     9901 taaaatctct aaagtctttt gttcccctgt ctgcttcaaa aatggcagtt ccatatcttt
     9961 tagaatcact aagatcaatg gggcgatttc tggagcatct tcttttctcg caaaacgaat
    10021 catagtttcc tctccttttc tccattctaa catgcagaaa aagaaatcgc aaaatcgtta
    10081 ttttaaaaaa tcttcaatct tttcagtaat gaaataacca tgagtaacga ttcttgccgc
    10141 ttcagttcgg caataagggc agacgactgg catttcttta tcattatgtt cgactacatt
    10201 attgacttct tcgtatgcaa atttttttcc gcaattaggg catttcattt tttccatgtt
    10261 atctcaactt cctttcatct ttactctctt agcttactct tatattgtaa aaaagagaag
    10321 caatatcctt cttctatgca gatatccata cctgacggtc ctgttctcca tctattaatc
    10381 ggactttata ataaggacgt tcatctttga ctagaagtgt ccactctttc acttttgctt
    10441 ttttcccgta gtagctgact gcttccttca tcgctgcttg cggttgtttg aactcttccg
    10501 ttttttctat atcaaaaaga tgttctgcgg ccttttccgt gtcatttcgt acggttgttt
    10561 tcccagtgga tggattgact gctatgactt gtccttcaca aaaaaactgg tattcatatg
    10621 ttgcattcgt aggattctcc aaatcttttt caactgcaaa atgaatagca gatatgttat
    10681 gttcttttgt ttcgtttttt acgattgctg ctgctttttt caatgagatc cgatattcct
    10741 ggtcactaat atcatccatg tgaaaagcgg cattgagcag tcccgtcata ccggtaatcc
    10801 ctattacgat gagggcagca acaccaaaca tcattttttt cattcttcat tctcctgttt
    10861 ctttttttta aagaatatca agaaatgaac gaatgaaaaa tcgaatgaaa cgaaaaaaaa
    10921 gtgacaattt tgttatcttt attcgtgcag ttctaatggc aatccgtctg gatcaaagaa
    10981 aaacgtcatt ttttttcctg taaaggtatc ttctctaatc ggttctgatt cgatccctaa
    11041 ggactgcaat tctgcaattg tttcttcaat atcgtctact ttgaaagcaa gatgacgcaa
    11101 gccacaagct tctggatagt ttggtcgttg tggcgcatta gcaattccaa aaatctccaa
    11161 ttcagcactt cctagtttca gatctaattt atagtcatct cgttctttgc ggtaattttc
    11221 tctgattact tcaaatccta atttattaac ataaaaatca cgtgatttct gataatctga
    11281 aacaatgatg gctatatgat ggatcgttgt aaggttcata tgagcactcc tattcttcgt
    11341 ttaagctcgg atctatatta atctccagtt ccattgtaaa tgtgtcagat tgaaaaaaat
    11401 agctgttttt cctttttata agaaaaaagg caaaaaaaac agaaagtagg tcctccaaga
    11461 cactcttcct gttcccaata taataaatga aagtaagaaa ttatattttc aagaaaacaa
    11521 acagaaaatt atcataacac aaattattta taattgaaag actagttttt tttcaagaaa
    11581 agcatacaat agattgtgtt agtaaataca gaacacacta catttagtgt cttaaggagt
    11641 cctcatacat gaagtcatat tttcagtttt tagctcatca gctaaaaggc tggccgcaac
    11701 aaaattatta tcttttcttt ttcagtcttg gctgccaggt catgactctt gtcaatagtc
    11761 ccatcacttg gctgtccgtt attacattta tcggtaccac gcttggtgta ttgtgtattt
    11821 tatccatcaa tgcggcaaaa tcagtcaacg gtattttagg tattttgtct gcgatttgtt
    11881 ttattatcgt tgggttttca gcaaagaact atttaagtat cggtgaacag attgcttata
    11941 tgatcacatt ggatatcccc gtattgttaa gtaaagaatg gaatcataat atggcttcaa
    12001 aaatccgaaa attcactgga aaaagctggt ttatcgcaat catcagtact ttccttgtct
    12061 atcttgtttc aggattcctg attggtcgtt atacagatga tcctcggccg tgggtagatg
    12121 ccattgcttt ttctattagc ttgactgctg gtatcatcag tttcttgcgt tacaacaacc
    12181 aatattactg gtggttagct tcagggcttg ctcaaatggt cctttggtat atttcattta
    12241 cgcatggatc tgcaagtttg gcgatgttcg tgaacagctc tgtttacttg ctgaatgatg
    12301 tccttgcatt taccgtttca ccttggtata accaaaaaga acgtgaacgt atgcaaaaaa
    12361 tggaagaaga atactacaac gcacaacaac acgcataaaa aaactgagcg tgggacaaaa
    12421 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaatc agaagcaact
    12481 ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct
    12541 tatttcttgg agttaaacgc ttctgtccca gcctctattt tttatagtaa ttccttcaga
    12601 tactttcttg cctcatcatt gaaacgcagt gattcttcta acttctgata atattccaac
    12661 gcttgtttct gagcttcagt aaaacgataa tgttttcctt tccatttgtc gattgtctct
    12721 tgcacatttc cagattggat atctttcact actagttgcc atttttcttt tgagactgta
    12781 cggtctttct tatcagcaga ttgtatagct tctcgttctt tacgcaatac ttttaaaaaa
    12841 ccatttctga ctatccgagg aatgctgtcg aagaaccctt ctgtaaaacc atagcgacaa
    12901 tattgcttcc aaataaatag atagattttt ggttggatga atcgttggtt tttcgcaaaa
    12961 tattccatct gagcagtgac ttctggtgtt cgttgacgtt ttgtctttct caacacggaa
    13021 ataaatgttt caaaataagc attaggatgt actggcactt cttcatatac gatggattgc
    13081 caatctttac gtaaaaatgc gcgatagttg atcaagaatt cttctacatc ctttactaat
    13141 tcttctgcaa agtcgtcaaa tagttctttt tttatctgtc gcttttttga ctgcccattg
    13201 atgtgttcac ttattttctt gatttcattt tccaatgctt gataatccgc tatgtaaatc
    13261 ggcatttcta cttggcgaaa ttctgcgagc cgctgagcta attttatatc cggtaaatat
    13321 ggctgtttca tccgacgatt ttggtacaag acaaaacagt atttttgcca tagtccttca
    13381 ggaaaatcga tattcttctg cttaagccat aataattcgt ctaaggccag atcaactttt
    13441 gtcattccct gatggatcga agctgccact gtcggagaga catgcaggtg atcggcaaag
    13501 tggttgatgc ctagttttag gatctttccg gtttttggcg atttaacgat gtattgcact
    13561 tttaattctc taccacactc acaatacaat ttttgctggc ttcgttttgt tcgaaaaaac
    13621 ggattgatct tgaagtcaga aaacacccaa tctgttgcag ctaaataatg ttcttccata
    13681 aaacgagagc tgattagata tcggcggtgc ttatcgacta gttcccgctg ttttttcgtt
    13741 aattttgcat agatctccca acgttgactg cttgtgtatt gtttagaaat tttgatggga
    13801 tagcttttgc tgatttcttt tggaaaccat tcttcaaaga cggattgcca ttcttccacg
    13861 gctgtttctt tcctttctaa ttacaaaaag ctggctacta acataacgtt agcaaccagc
    13921 cagtgtttct tgctaaatta gtgagttgtt ccggcatttc cggaaatatc ttctttattt
    13981 tctactgctg cttcaactgc ataagtcaaa gaagaagcaa tgtcttctaa agacatagaa
    14041 ggcgtaccca ctggacgatc gatcacttga tcgggtgaga atggcacgtg gatgaaccca
    14101 cctcgaattg ccgggaattg tgtttcgatc aaatgaagca aacgatacat gatatcattg
    14161 cagacaaatg ttcctgctgt ataagagatg tacgctggta gaccatgatc ttgtacattt
    14221 tttgtcattg ctttgattgg caatgaggta aagtaagcgg ctggaccgtt ttcttctaaa
    14281 gccgtatcga atggctgatt tccttcatta tcagggattc ttgcttctgc tagattgatt
    14341 gcgacacgtt cgaacgagac tgctgaccgc ccacctgctt gtccgacaca gatcacgaca
    14401 tctggttggt gctgttggat ggcttctttg actacttgac tgcttttttc gaatacggtc
    14461 ggtacttcca ctttgatgat ttcagcaccg ctgatttcat ctggcatttt ttttactgct
    14521 tcataggctg gattcacttt gtccccacca aatggatcaa aacctgttac tagtactttc
    14581 atctgttgtt cctccgtccg attgatttaa catatggtta tcttttatca taccattttc
    14641 cttgaacagc gtaaataaag gaaacaatag tttaaataac ataacagatc aaacttaata
    14701 tattcttata aaaaaatagt gttccactac aatgttagaa gaggttgcga caattattct
    14761 gtcacaaccc ctctcttcta ttatttaaat agaatcatat aagcaatttg gaaaactagc
    14821 ataaataacg caataaacaa ctggtttttg atcactccgt atttgtcttt catctccagc
    14881 atcgctacag gcacgatatt gaagttagca gccatcggtg tcagcagggt cccacaaaaa
    14941 ccgcaagtca atgcaaccat tcctattaac gctggattcg ctccatgact aaatacgaat
    15001 ggtgcaccga tcccaaccgt catcacggtg atggccgcaa atgcattccc catgattacc
    15061 gtaaatatca ccatgccaag agcgtaaatg atgatgccga tgttcacatt tccttctgga
    15121 acgatcgtac ctactgcatt ggaaatcact gtcccaactc cagcgcttgt aaataccgca
    15181 cctagagaag ctaaaagcat tggcaacatg cttaacggcc ctactgtgcc cagcatatct
    15241 gctgcatcat ctaaaaatgt acttggtcga ttatctcgag aaaagaacat caagattagc
    15301 atggctgcaa aaacaccgac gcccacgccg actaaagcac ctaaattggt aaatagagca
    15361 aacaagatcg caaagacacc gatactcaaa gctggaataa aaattttcat tcctagtttg
    15421 tctgacattt tttccatata ttcttttgat ggaagccggc tttctccttt gctgactttc
    15481 ttagcaattg ctggtatggt catcgcaaag atcaagccac cgttcacgat tgccggaagc
    15541 catcttcctc ctgcaataac tacccctaag accgtccaga aaaaggctga cccataacga
    15601 tgaggattgg ttttatctgt taaatttctt actccagtgt aaatcaatac gagtcccatc
    15661 acgatataaa ggatctccag taatttatcg cctaataaaa tatcaggatt tgtaaaaaag
    15721 tccatcttat tgcccctttc ctggataata tttcttcgct aatttctttt ctttaagata
    15781 gaagtagaca cttgccacaa ctaaagccgt gatagccact ggaacttcca ctaaagcaag
    15841 ctgtcccaat gttacgtcat atcctagatc tttcagtgtt gattggacta gcaacgcccc
    15901 tgctcctccg atgaatagta cttggccaaa gaaccagcag acattttcca tagctgaagc
    15961 catacctttt agttcttctt catgcttagg atggacttct aacccttttg attcgatggt
    16021 ccccaatgcc atcggtaaaa tgattggacg aacaaatcca gcaactccac caaaactcac
    16081 gttaaaagcc gcaaagatca ttcgaaagat cccataaata tctacaatca taccagcaga
    16141 tactttcttg aatcgcttga tcaaggaagc cgctgattct ttcagaccat ttctttcaag
    16201 cgttcctgtt gccaacataa tgatgataaa aatcgccatc ccgcgattgt ttacaaaact
    16261 tgtacctaaa agttctaaca ttccttgaat gccaagaccg ccagtcaaag ctgtaacaac
    16321 taaagcaacc attacgatca atatcgagtc taatttaaat gcaaacccga gaataacaat
    16381 caagacgcct aaaaggctaa gataattttc catatatttt cctccataaa atgacaatat
    16441 ttttttaatt ataaagcaaa acacataaaa aatatagttg ttttctcacg aaatagacac
    16501 tattttta
//
LOCUS       DANKAZ010000053        16490 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000053 DANKAZ010000000
VERSION     DANKAZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            38..1450
                     /locus_tag="PWY67_001728"
     CDS             38..1450
                     /locus_tag="PWY67_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2057998.1"
                     /translation="MSQLLIATPFHVLASENSTYKIAKERTETTEMSNSDVTAESSEL
                     LTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESST
                     ISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQS
                     QPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQL
                     AQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLT
                     KGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEV
                     KGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFH
                     YSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEIC
                     KDSSLSQSMLVDPKTLLFGD"
     gene            1799..2431
                     /locus_tag="PWY67_001729"
     CDS             1799..2431
                     /locus_tag="PWY67_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDL2057999.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3019..4458
                     /locus_tag="PWY67_001730"
     CDS             3019..4458
                     /locus_tag="PWY67_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2058000.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4753..4971
                     /locus_tag="PWY67_001731"
                     /pseudo
     CDS             4753..4971
                     /locus_tag="PWY67_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5220..5435
                     /locus_tag="PWY67_001732"
     CDS             5220..5435
                     /locus_tag="PWY67_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058001.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5652..5908)
                     /locus_tag="PWY67_001733"
                     /pseudo
     CDS             complement(5652..5908)
                     /locus_tag="PWY67_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6095..7006)
                     /locus_tag="PWY67_001734"
     CDS             complement(6095..7006)
                     /locus_tag="PWY67_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2058002.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7025..8029)
                     /locus_tag="PWY67_001735"
     CDS             complement(7025..8029)
                     /locus_tag="PWY67_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL2058003.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8026..10149)
                     /locus_tag="PWY67_001736"
     CDS             complement(8026..10149)
                     /locus_tag="PWY67_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2058004.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10154..12601)
                     /locus_tag="PWY67_001737"
     CDS             complement(10154..12601)
                     /locus_tag="PWY67_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2058005.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12588..12977)
                     /locus_tag="PWY67_001738"
     CDS             complement(12588..12977)
                     /locus_tag="PWY67_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2058006.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13037..13540)
                     /locus_tag="PWY67_001739"
     CDS             complement(13037..13540)
                     /locus_tag="PWY67_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2058007.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13553..13777)
                     /locus_tag="PWY67_001740"
     CDS             complement(13553..13777)
                     /locus_tag="PWY67_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058008.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13881..15791)
                     /gene="ltrA"
                     /locus_tag="PWY67_001741"
     CDS             complement(13881..15791)
                     /gene="ltrA"
                     /locus_tag="PWY67_001741"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2058009.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4912 a         3271 c         2939 g         5368 t
ORIGIN      
        1 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
       61 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
      121 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt tgacaacttc
      181 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
      241 tagtacgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta gtacgacttc
      301 aaatgccgag tcttcaacaa cttcgtcaag tgaaagtagt acgatttcaa gtaccgaatc
      361 ttcaacaact tcaagtagta caagtgaaat aacttcttcg aaagtgacaa aaccaaacca
      421 tatagaatct tcaaaaaagg aagcgccaaa aacggaagga caaacaaaaa ctttttcgac
      481 accacagcaa atagaatcaa cagaatcttt ccaatcacaa ccaatactat ctggtaatga
      541 agaaattcat tttgacaaaa atgaatctgt agaaaatttt attatgaaaa ttggtgagtc
      601 tgcgagaaaa attggtcaga aatatgattt atatgcttct gttatgattg cacaagcgat
      661 cttagaatct gccagtggtc aaagccagtt agctcaggca ccgaattaca atttatttgg
      721 gataaaagga acttataatg gaaactttgt cattatggta acaaacgaag atttgggtaa
      781 tggaacgctt tatacgaccc aatcaaaatt tagagtgtat gaaaactatg aggaaagctt
      841 tgaagattat gccaaattat taactaaggg aattagtgga aataaagatt tttatgcagg
      901 agcattaaaa gctaatagta aaacatatcg agaagcaact aaattcctta caggacggta
      961 tgcaacagat acacaatact atttgaagct aaatgaatta attaaaacct acgatttaac
     1021 caactatgat aaagaagtta aaggtccaca aatgagtaag actggatacc aagtgcctat
     1081 tcataattat tcaataacca gtttgtttgg aaatcgtgga aatgaatttc accgaggact
     1141 tgatatggca gctacacagg gtgagccgat tcatgcagtt aaagctggta aggttgttaa
     1201 agcagagttt cactactctt ggggaaatta cgtggtgata caacacgaag atggatcaac
     1261 cgcgttatat gctcaccagc aacaatacct agtaaaaact ggagataatg ttttgcaagg
     1321 acaaataatt ggctatgtag gaagtactgg gaatagtact ggcccacacc ttcatttgga
     1381 aatttgcaaa gatagtagtc tttctcagag catgttagtt gatccgaaaa ccttattatt
     1441 cggagactaa aaaaggtaat caatctgaca aagtaacaag aaaatggttg gattcttaga
     1501 cctttgatca ggatattgag actggaattg atatctcgat tacgatgggt ttgacaaata
     1561 aaacaagtcc attatcgaat atcgagtgta ttattgccgt ctttatgctc atattttgag
     1621 caggtttcac tagatgaaaa caatttatct atcttgagga tttcgtgtac gtatcgttca
     1681 gtcttgtatt gtaaattagt cataaaatca gactaagaaa aattggaaat atttttttct
     1741 gatttattaa aagttaattg aaattatatg gtacttcttt cttgggaggg taggtgcagt
     1801 gaaggaaaga tatggtcacc ttactgttgt tgaggagatt actcataatg gatataaaaa
     1861 atatcgttgc ctatgtgact gtgggaatga aacgattgta tatcgatcaa acttactatc
     1921 aggtagaacc aaaagttgtg gatgtggaaa ttataaaaat aggcataaat ataaaaattt
     1981 gacaaataaa acttttggta agttagttgc aatcaagcca actaaactgc gaagtgaagg
     2041 aactgttgtt tgggaatgtc aatgtctgtg tgggagaaaa aagttagtat cggcaaagcg
     2101 acttctaaaa ggtgatgtaa aaagctgtgg atgtttgaaa tcaaaaaagc atcacattga
     2161 aaataaaaaa ttcggattac ttactgcaaa gtatcctttg gacaataact gttcatggaa
     2221 aacaaaatgg gtttgtcatt gtgagtgtgg cagtatgtgt gagataagtt atagtaactt
     2281 attttttgga catactcaaa gttgtggatg tttaaaaagg caagaaacaa ggacatgtgt
     2341 tgaagggaca gtaatagagt gtctaacgtc aaaattaccg aaaaataata caagtggtgt
     2401 taagggagtt tatcgttcta aagagaaata gattgcgtat ataactttaa aaaaaaagag
     2461 gtactattta ggatcgtatg attctattaa gagtgcagct caagctagaa aagatgctga
     2521 aaataaattg tttcaacctt tgattgacaa atatcgaggt aatcattaat tattgtggat
     2581 aataattaac agtgatatat aaacatctat aatatattat tcatactatt gatatttagt
     2641 ataatcctat ggaattgaat atcatagcaa tgtatttgaa aagcaaattg gaaaacaaat
     2701 aggtgcaaca ccataaatat gaaaaaattg ggaaaaagta gaatggaaat ttgcttacgt
     2761 ctcacgagat agctcacact attaatctga agaacaatga agtcgacttt tagaaattgc
     2821 tagaagaaag taaagcgaat tatcactagt tactatagag taagttcaaa taaagcatct
     2881 ccaacttaca tggttacatg aggatatcaa atcgaaatta ctcaagcttc ctatggctag
     2941 agtcgcgaga agttgttggg cagtagacat aggttatatt gttcttagct acacgatcat
     3001 tagaaaggaa atcctattat gtatagttat caagaagatg caaaagtcca aaaaaatcgc
     3061 tggtggattt tgattgctat atctatgttt accttcatgt ccacattgga tggcagtatt
     3121 gtcaatattg cactacctac catctcagat gatatgggca taccaatgaa tcaagcggaa
     3181 tgggtagtgt ccgtttatct gatgattgtc tgttcttgtt tgttgctgtt tggtaaaatt
     3241 ggcgatagcc gtgggaaaat caaagtattt cggattggta cgctgatctt tgtcttggga
     3301 tcgctgttgt gtgggttcaa tcattcgcta ggtattttat tgttcgcacg ggtggtacaa
     3361 gccattggtg ctagtatgac tatggctaca aatgccggaa tcatcacgga agtctttccc
     3421 atacatgaac gtggtcgggc attgggagca ataggtgcgt ttgtttcatt aggctcgatt
     3481 gctggaccag gtcttggcgg cttgattttg tcccaatttt cttgggcgta cattttttgg
     3541 atcaatgtac cggtgggaat cgtaacgatg ttgattgctg aaaaattctt accaaaagat
     3601 atcaccatga gcggccatac aatcgatgga gggggctttg ctgccattgc ggctacgatt
     3661 atcagctttt ttggagcaat ctttttgggg caagaaagtg gctttttcca tccagtcccc
     3721 cttgtactgt tcgtaatttc agtgattgcc tttgtcagct ttttaaaagt tgaaaagaaa
     3781 gcagcctctc cattgatttc tttcggtatt tttaagaaca agatttttac catgagcttg
     3841 attacagcct tgttgatttt ttcttccaac ttctttgtca atgtagtgat tcctttttat
     3901 ctgcaaaatg ctcgcgggtt gagtcctagc tatgctgggc tgttgatgat ggtctttcct
     3961 ttgatgatgg tcgtcggggc gcctttaagc ggctacctga ccgaccgggt tggcccggag
     4021 attttggtat tagtaggctt gggcttattg tcggtaaccc aggtcatgta tatgtttttg
     4081 tcagtggact ctcctttgtg gctatacatt gtcgctactg ccatcatggg tgccggcaat
     4141 gccttgttcc agtcccccaa taatacgatg gttatgagct cggtcactat cgaaaacttg
     4201 ggactggcag gctcgatgaa ttcctttgcc cgaaatttgg ggatggtcag cgggatttcg
     4261 ctttctacga cggtcttata tcaagcgatg tctgctaaga tgggccagca agtaactaac
     4321 tatatcgcaa atcggccgga tgtcttttta tatggaatga agattacctt tttaggcagc
     4381 tttgttttgt gttttgttgc ctttttcctg actatctggc gtatcaaaaa acaaggaggt
     4441 attcagaaaa atgtctgaaa aaaccactgt tgaagaattt ttggaggcct gcgtatgggt
     4501 gagtgggatt tccatggatg atggaagatg aagtgaactt ctaaaagccg aaaaactcaa
     4561 gtattatctt caaaatacta gtgctcaatg agatgcttga tttgatcaca agaagactgc
     4621 gaaagccgct ggcaaccgca agctaggtat cccaccgatg gactacgttt tagaggctac
     4681 tggttatgaa tacggagcca acgctctcat ctgcataaga atacatcacc cagattatgt
     4741 aaagattttc gatacttctg tcaaaaaatc attaagatag ataaatgatt tccgtctagt
     4801 caaattggtt cagaatgtag acacacagat ggtaagaagt cactcacaat tcgagaatgg
     4861 acttgtccta tttgccgtat acatcataat cgaaacgtca acgatagtat caatattttg
     4921 accaagggct atgaatacaa gcattgcctt agaaaacatc aagaacctta ggaacaacgg
     4981 ggatagcttg gttaataaga gataccgctg tttaggcttg caagtatact cttttatcaa
     5041 gaattccgtg gctttagtca tgagaggctt aatactatga aaattttctt tagcattttt
     5101 gatttaggtt ccttttctaa ttataataag atgaccacta cagtaaccta ctgaaatata
     5161 taaacagcca ctgtagtacc attttcaaag tagtctatga gaggagaatc ttcattctta
     5221 tgaacgctgg aattgcggat atgaatttaa taaaaaaaac ccttaatgat tttacttcca
     5281 attcaatatc aaaaggtact ggtatcaatt taagcacaat aaaaaagctg aaaagtggtg
     5341 aaagatcagt ggaaaagctg aatcttttag atgctataaa aataacggaa tttgctatga
     5401 aaaatggaaa agctgagatt gaaatatgga gataattatc gtctagtgaa ttacttgcca
     5461 cttagctgaa tttaacgatc cttggtgctt gaagggggag attcttggga atagagcata
     5521 cttgcaagcc tatgtcttta ctaacaacga tatctcttat taaccaagct atccccgtag
     5581 ttctaaggct tttactttta tctaagcaat aatcgtattc aaaggaaatt tttgaccctt
     5641 agaaagtctc attttatcag acgtgagctt aggcattgct gaattttgtt aattcggttt
     5701 taggttgtgc tttgagcata atatgaaagt attctttatc acgatttcat tcatctaagg
     5761 taatgtgatg tgattctgaa attctttcaa aggcagtttt tgcataagct gacattacat
     5821 catcaatgac atgtttacaa tgtttcacta ctaaaataag ggggtaacca agaagaaaaa
     5881 ctgaatgatt atttgtatct aattccatgg tatatcaacc atttcctata tagactgatt
     5941 atgacatgga ttaaaataaa aaggcactat gtcttaattg tcggttttcg agttaccgaa
     6001 tggcattcat cacccaccta tagaggatgg gcgacttctg cctgaaataa gttacactac
     6061 ttataacttc taataaaatt agattgagtc attattattt aacatcaacg attttccagt
     6121 ttccattttt ttgtaatact aatttgtact gaaagtagct agtggttttt gatacattat
     6181 caaggtattt tatctttgta ctcactagaa aattttttcc gttttgtaga taaactggat
     6241 tctctaactc cgaaaacttt aagtcaatat tgattggtcg catcgcacca ttttcaacat
     6301 aatactccaa ttctttttca gaagctgatg gataaagttt aaaaaatgtt tctaaaaaat
     6361 cggtgatttc ttccgttgtt ttggcatcaa tttctgaatc gttttctact tgttttgctt
     6421 catattttgc cttgtctggc tttccaatca ttgttggcaa actagttact aaatgattcc
     6481 tgttttcatc ttcaaagatt gtcactcgat atgcagaaga aattgattgc gtcttttttc
     6541 cctctgtaat atgttgctcc actaaaaatg aaacgacaaa ttcttccgag tgtttttcaa
     6601 catctagaat ttttacagat tgaacttcgg aactcgtggg aatatctgct cgaaccatat
     6661 cttggattaa agcgagaccg ttttctgtaa ggtactgttc gagattactc attctctttt
     6721 caattgcttc tttgttattt ttccatgaaa aatattcttt cgcaaaatcc tctgtaaaac
     6781 tttcaacgcc agaagtattc actaccttct cttttataac cactttttca tgagttgtat
     6841 gttgatcaat ggcggtgaaa tttttataaa cgccaaaagc aagactacaa gacaataaaa
     6901 gccaaagggc aagcaccatt tttcgatgct tacccacgga tacaactcgt tcttttttct
     6961 tttttgggct cttttccttt tgattccgtt caatttttat cttcattctt tttcctcttt
     7021 tctcttattt gtatcggcca gctccgataa gatgctgccg ccaatagttt tccgttagat
     7081 tcgtccaccc cactggatct cctgcatgaa acatgcgatt ctcccctgcg taaatgccca
     7141 catgggtcac ataagtaccg gcatcatagg ttgaatgaaa gaaaactaaa tcacctggtt
     7201 tggcatcttt tagatcaata tgagtcatgg catcatattg atcctgagcc acccgaggaa
     7261 gtttcaggtc aactttggca taacaccatt gggtcaagcc actacaatca aaccccgttt
     7321 ctggacttga gccaccaaac acataggccg ttccttcata cttaaatgcc tcatcaaaaa
     7381 tttcttggac tattttgtca tcaaactgag ttgtagttag gtattgcttc actaataaac
     7441 agtaaaacat attgccataa ttataccgcc acccaccatt ttccttaatc gcgattgggt
     7501 tggggtaatc gactttttga ccacctgatt tttcctttga aaaagattca gctaaagcaa
     7561 aactatgctt cttcccgttt ttcccgacat gatctagata cccgccacca tagttatagg
     7621 cctgaataat actatcttga tcacacccta gttctttgga acgcttaacc aaagacgcaa
     7681 aatagagaca gccttgtttg atggatgcct cggtacttaa tgaattaggt ggtaatccgg
     7741 ctgactcaga actttgcata acgtcttcca ttgtgccacc agattccact tgaataatcg
     7801 ccaaaagaat tggaatatac tctgttatat ccgattcttt ggcatacttt tccaccattg
     7861 cacggtgttt caatacttct tctgagacac tagttccgcc aggagaagtg ccagaactac
     7921 catttccctc actgtcttca ccaaacaaca aacccacaca catcaaaata cccataaaag
     7981 agatggtcag aaaagttaat gaaataacac ccactttctt caagttcatt ttttctcacc
     8041 tgaccttgat tttggacgat ggttcattct tttttgtact ttttgattgg tctgctttgg
     8101 cagagtggac ctctgtttca cttgttggaa agaatttttc ttacttgctg attggctttt
     8161 cccttttgaa atgatttctt gactagttag ttttcgtttg atttcgggat tcagatttgc
     8221 tctcacaatc cgctttggtg cttctttttt cagcatcata tctttctgag gttgagctgc
     8281 tttatctatt ggaatattcg cccttctttt tccctgacga tttgttgctt cactcagtat
     8341 cttgcgtttt tcgtccattt tcgcatttct tcgttgtcgt tgtttttctc gcaaatccac
     8401 tcgatccgct ttttcttgga acagtccgcg cttgaattct tctggtgctt ttttcgactt
     8461 ttcgattcct ttatggagat tgtatttcaa attcgttggc gtattcctta cctgatcctt
     8521 tacttgttta gtcttatctt tcaccaaagc ttgggtatcc aaaactttgc cgatttttct
     8581 cccagccaaa ttcattcggg attgtttctt attatttgaa actggattct ccttagttaa
     8641 gtcagatagt atttcgtgat cgttcggtaa tcgtgaattc tttcgtagag aagaaccgga
     8701 tggcttaaat ttggattttg attgttcctt tgccaactta tttccgactg ctgcaccacc
     8761 accaaaagcc aaagctcgtc ccacatttcg attgagttgc cgcaattttc gattcattaa
     8821 catttgcggt tttcgcatga cgcgtcttcc cattgactgg ctatcattac tctggaggtt
     8881 gaacatactc ataatgtcgc ccaatttgaa atagataccg gcaaaggtca caatttgcag
     8941 aaaagcaacc atgaaaaatg gataggttgc cgaaatattg aaaaacatcg tggagatgct
     9001 aaatgcagtc gtaatcacca aagtaacacc cgctcgcatc ataatcgtat tgaataaacg
     9061 aataatcgcc ttctttccta aactttcata agtcggcaac atggacaata agaaactaat
     9121 tggcagaaac atcgcaaaga taatgaagag aatttgcgag aacaacataa tccctgttag
     9181 aagaaaaaca aagaaggaaa tgccaatatt gaacaatacc aaaaagacca cggtccctaa
     9241 acggttcatg gtcttggtaa tactgagatt cgcattgtca ttgtcttcaa tctctgcctt
     9301 gaccgcttct tcccgatctt tgcctttgtt ttcatctgga ctcacagata gaatcttgtt
     9361 gacccggtct tcaccaattt cttctatgtt ggaatcatca aattgcagta gtaaccaagg
     9421 ttgttggact tgaatcgaaa acaaactatc ccgaatcaaa tccacactat cttttccttg
     9481 gctttctgaa ttgggtacaa cgatctcggt tccaagggta agggccgctt cactaacatc
     9541 tgagctaaag tcattgattt ttgtgatata ggtaggcgca taagcaatga acgaacccga
     9601 tagtagaaaa atcatcacaa aattaatcac ggctcgaact gctttcgtgg tttctcgttt
     9661 taataatccg gtatacgcca catacacccc cagagctaaa atcatcaata atagaaaccc
     9721 aaaataaaat ccggaggtct ggagtccatt ttcagtaata ccagccaatg tttggatatt
     9781 ctttccaata ctttcagccg tatccgaaat gaaatctaat ttgtaagcct cttgaaccac
     9841 ataacccgtg gcgttcgaca aatacaaact caccgtccaa atgaaattag taatggcata
     9901 aagaccatac atcacgcttt tccctaagcc atcaccccaa ttccaaggca accaatccca
     9961 agagctatcc acaaagaaat ctagttgata gttgttcagt gaatatttgg aatatagatt
    10021 tcctgcttca actgtatcat caactagccc tgtcgcctcg gctaccgtac caactaaact
    10081 caatagaact atcagagtga gtatgctaat aaaaacgatt cccacaatcc gaaaaagttt
    10141 ctttttcatc ccctcactcc attctctctt gaacaggtgg acgggtatca aaggcatgga
    10201 agagttcttc aaagactgga tgtacctgaa ccacgcctac ccgaccatac aaatcctgca
    10261 tcaagcattg gccattttcc aaatctcgta ggcgtttttg attgctttca tcttctcgat
    10321 ccaagccaaa gaattccaag gttttcttga tttcatgaat atcggtactg cgaaacgcaa
    10381 acttcaaccc gatattattc ttcaagcgtt catccagtaa atcatcacta ttttgcgtga
    10441 caaagtaaac cccggcattc atagcccgcc cagcacgaac gagcttattc gataatgttt
    10501 tcccttgcgc tacttgaaga aaactccacg cctcatctaa gtctacaatt ttgaagattt
    10561 ctcgattact atggataaag tccaaggcaa aagtggaaat gacgattagc atggaaacac
    10621 taagcaattc catggtggta tattcttcaa aagtggtttc tgcatctggt aaaaccaaat
    10681 ccgctacttg aatgatattt aactgcctat ctaaactaat cgactgggta atcgaaccat
    10741 ctgagaaaag caaatgggca aaatcataat cggtaaagga ctcgatgtga tccgcgatat
    10801 tttccgcaac tggtgagcct tcttttcgaa gctcattaat tacttgtaat aatccccgtt
    10861 gattgctttg ggtaaccgtc cgaatggcct tacgcaacac tggaaacttc tccccatctc
    10921 gactagaaat tcctgttaaa aaggtcagaa tatcaattgc tagactttca gaatccttct
    10981 ttttctccat aatcacataa ggatcaagta atccttgatt ttttggctta ctagttaagt
    11041 tgagaatatt gatttcatcc gcaatttctg gcaaggtttc tttccagcca ccccgttccc
    11101 cttttggatc gacaatcaca gcttgtccac caaaaaggac ggcgtaataa actaagagat
    11161 tgttactaaa ggactttccg ccaccgagtg aaccgaggaa tgccgctgct agcgcattgg
    11221 taactgaatc cttgacccct tgtgaggcca atgccggttt caggtaaaca ttgcgccccg
    11281 tatctacgtt ataaccaaag taaatgcctt ccaactcccc taacatctga gtggcgccaa
    11341 aaccaagtcc ggcgagaaaa tcagacgtaa catactggat ataatcattc atgtatcgct
    11401 tactagaagg taagaattct tcatgtagcc ccatcatatc gccaaaaggc cggactagtt
    11461 ttacattggt atcatcataa aaatctagca cttcatcaca ccggcgcttt agttcatcta
    11521 ctgattcggc acttactcga accacatagc tcagtttgta catggattct ttggactgat
    11581 ccaaggtagt ttccaattca tctacggaat ccaatgcatc taagacattg gaatttgttt
    11641 cattgtcaga ttgataggca tggttgtcca agtcttttaa ttcctttttc ttattgcgaa
    11701 cggtagcaag tgcctttttg ttcgtcacaa tctccacatt catactagtg tcaattggga
    11761 atgtaaattg ttgttgctgg taatagaaaa gttctgagga gggaaactcc atttccccca
    11821 caattgtatt aattgttaag tacgctacat atgattcatg attctcatgt tccatgcata
    11881 agtaccgggg cttttcttca atcaaacaac gactgggacg taataagtcg taacgtttca
    11941 ccaaagtttc cgacttcagc tttttctttg gtagctgaaa ttcatattct tcaaatggtg
    12001 tacccttttc gccatagata tgttcaatca aatacatcag atcactcggt gtgactcgtc
    12061 tgactttaaa tcttcgagcc aatttacttt ccattagttt ttcaagcttg gtatatcggc
    12121 gaatttcttc attggataag gaaacaaaat cgcccatcaa atgatgattc actccataga
    12181 caaattcttg aaagccggag aagaaagatt tcttgagatt ctttagattg acctcttcat
    12241 ctgtcgcaat cagtttgaag ccaataaaaa agcgataatc aatctgacta tcgccaatca
    12301 tggatactaa tgcctctgtc tgtaaatcaa ttcgttgttt cgctacttct gttaatcgcc
    12361 cggtaatttc cttttttgat cgttcttgcg tggcacgaat actactttct gtggcaattt
    12421 gtaaggcatg aatcttgcct tcacgatttt gcgcaatcaa ttgacgaaag ttatcgtgga
    12481 cttgatattt ctgttcaggt gacaaaaagg agtaattgta aggtattaac tcatagtagg
    12541 caaaacattc tccctcttga ttaaagacaa gattattttc aatatactta atcgggtaca
    12601 tagttcacac tccttaccag agtaatgggt tcgtcccatc gtttcttctg ttctttgact
    12661 ttctttcctg cgaaagtgac cttaaatcgt aagtgatagc taataaaaga tcgtaaaaat
    12721 cccaatggtt tcttaccgtc aaacgttttt tgcgacatga accaagtaac tgccaccggt
    12781 atacctaagt attttaaaaa ggccccctct atcatagata gtggaggcac atgggcaaat
    12841 acgatgacaa acagcaagga taaaacaaac caagccatct ggttaaaagt cacaggaaaa
    12901 ggcagttgaa agtcattaat tgcataaatg accttttcaa ctgcccaaat actggtgtag
    12961 ctttttattt tcttcatcaa atcattcctt taaaaagaag gatagtttag aaatcaacta
    13021 cccttcaata attttgttaa tatggatact caaaaacacc tcgactcgtt accaaataat
    13081 tgcctccaat ctccaagtct ctaccacacg catcaaaatc aatatacgtc ttcaagtgtt
    13141 ctggtacttc tccaaaatca ccagcttcta caatcaagaa atatgctaca tcactcatat
    13201 catcgcaatc tggataatag ataatatcat ccacatgctc taccatctcc tcaaaagaat
    13261 tgaagaaagc atgttgaatc tcagaagcaa cttcaccaac tggcgttcct tcaagttcct
    13321 ccgctaagcc acacaaccga ttaatttcct caattggcgt atactcatca atctcaaagg
    13381 gaagttcata atcatgaatt gcatattcct cgtattcgtc atttaaccca attcgttctt
    13441 tcacttcatc aaaatcgaca ggtggggtaa accacgcccc caccaattca ccttcgttat
    13501 atttcccgag gttcgcaata taaacacgca tctgttccat cgtgcttccc ccttacgcac
    13561 cgacaattcg gttaaacaaa ctgagtaaga catccttcac cccagaagca ttaaagacca
    13621 atccgaccgc aatcaaagca atcaccaaaa agccaatcag tttggaaaac tcccgcttaa
    13681 atcctaaaaa caaaccaatg accacaattg ccagtaagac taaggattgg gcattggtta
    13741 aaaaccattg atatatggat aggtaagttc ctgatattat aaatatctca aacttttccc
    13801 cccattcaca cggagcgtgc gactttcacc gcactccgcg ttccatcgtg tttatattga
    13861 aaagtattta tgtagtagac ctatatcaag tttaattctt gattgccagc taatactcta
    13921 tatttattta gtcttatgag ctggacttta tttagcttca atagagctat atatttttgt
    13981 attgtttctt ctgttttagc gtgtattaga cggtgtaccg cctcttttac caaaataaga
    14041 tttttataat cgtcatttcc accagctcca gttggtacaa tatgatgaca atgtatttca
    14101 ttagcttgtt gaaattcttc cagagttata gcacattttc catgttgagc agaaaatagc
    14161 gaaattcgat tgtctgcgta ttctattgtg cgattttcca aagataattt cattaactgg
    14221 tacatcagta atttatcaac acttagattg gtgtggataa attttcttcc ctctagagtg
    14281 tagcaattcg ctaaatacac ctttgccatt ggttttctgt gttggatata tccaacaggg
    14341 aaaataggtt cgccctcgcc agcaacatac cgaagcattt tagatttacc atatcttgtt
    14401 gcctcctttg tcgtaagttg tctgcctgtt tttctcaatc tgcccctttt gtttgtacct
    14461 aatctgttag tgaagatagc cattacagct ctattcagtt tcatacaatc cagatttaca
    14521 ttcgttgcaa tctgataata attttgcata cctaatacca ttgaattata aagtcttgtt
    14581 tcttctaatt ccgttttccc ttttgaggga gaagcaattt tctttgcctg tgataccagc
    14641 ttgtcttttt ggtttttgaa ttgtttatca cttatatgtg atttaacaac taacttcttt
    14701 cttctcggta tcattttaat cttgaaacca agaaagtcgg agtatctttt tctaacattt
    14761 acaattcttg ttttttcttg tgatacttcc aattttaatc gttgttcaat ccattgagtg
    14821 attgctattt tggctttttc agcactttcc ttgtagcggc agaaaactct aaaatcatct
    14881 gcataacgaa ccatatacat ttcctttaac tttgttttct tcatttcttt gtaagcgttg
    14941 cttttacatg ggctaccgct tttattgata tgaaccacat agttttttgt aacaggattt
    15001 tcctgccatt ggctttctac ccaatggtct aactcattaa gaacaatgtt tgctagtagt
    15061 ggggatatta ttcccccttg cggagttcct ttctctggat atgttattgt accgttagtc
    15121 attttgattg gagcttttaa tatccttttt aaaatgaata tcaagtgctt gtcccgtatt
    15181 cccatagccc atatctgttt tatcagcttg gaatggttca cgttgtcaaa gaaaccctta
    15241 atatcaaatt caatcacata atgtagctgg ctgatttgta ggcggttata ggttgctttt
    15301 attgcatttt caactgacct attaggtcta aaaccatagc tgttttcact aaatttagct
    15361 tcgcagattg gttctaatac ttgttttata cattgttgta taagcctgtc ccacatacaa
    15421 ggaataccta gaggtctggt tttaccattt ggttttggta tttcttttcg cctcactggc
    15481 ttaggtcgat acccatgtgg actgcctgca acaatgtatc ttactttttt gacaactgtt
    15541 tctgctggca atttgccaat atctttaatt attgtaccgt ctgttcctgc tgttttactg
    15601 cctgcatttg ctttgatatt tcgatatgcc agtaatatat tttctctcga taggataata
    15661 tccataagat gagtgaaaat atctccattc ttacttttgg catataaatc atcaaaggtg
    15721 ttttgcattt cgtaatattc agcgtgtctt aaatcttcta cacacaatgt ttcattttta
    15781 tttttaggca taaggcttca ctcctttact tgaaattgag tgagcttttt tatcttactc
    15841 gtaatcctta ttgaaacaac ataatacttt tctaatatat acacgacttg agggcattcc
    15901 tccattctca ttacaagaat ttcttaggtt cgccctctgc ttttcagcat tagttcaact
    15961 gcttattgtt cttcgtcagt ttattctctt gatgagaacc caataccttt cctcgttccg
    16021 ataatcttat ctatacatat atatccttag gtcactagct ataagcctgc tggcttgata
    16081 ccgcctgtaa cgttatatgg catttcataa cctatattct tactttgcca caccagctac
    16141 gatttctcgt acctttgcat ttctacaaag ttcaaattta gacccgtaca ttcgctgttt
    16201 cgtcagttcc ttatagaaca ttctaaccat agatatttta tagacccccg acctgttgga
    16261 tacgctaccc acctctggat agttttcgat agaagatata tgttcatcaa ctattacggt
    16321 atctttcagc cgtctttacc gagcttcaaa cattctgagt ttcctcatac aatgcttgtc
    16381 ggaggctcag ggcaggcgtt tcagagcgtt acttcatcat tccacctgtc agattatcag
    16441 ttctcctaat aaccaaactt tttcgttctt ttattagtgc cttagctttt
//
LOCUS       DANKAZ010000054        16490 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000054 DANKAZ010000000
VERSION     DANKAZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(27..725)
                     /locus_tag="PWY67_001742"
     CDS             complement(27..725)
                     /locus_tag="PWY67_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL2058010.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="PWY67_001743"
     CDS             complement(718..2070)
                     /locus_tag="PWY67_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL2058011.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="PWY67_001744"
     CDS             complement(2293..3693)
                     /locus_tag="PWY67_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL2058012.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="PWY67_001745"
     CDS             3854..4591
                     /locus_tag="PWY67_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL2058013.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="PWY67_001746"
     CDS             4716..5195
                     /locus_tag="PWY67_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL2058014.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="PWY67_001747"
     CDS             5192..5959
                     /locus_tag="PWY67_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058015.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="PWY67_001748"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="PWY67_001748"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2058016.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="PWY67_001749"
     CDS             complement(6862..7464)
                     /locus_tag="PWY67_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2058017.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="PWY67_001750"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="PWY67_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="PWY67_001751"
     CDS             8145..8729
                     /locus_tag="PWY67_001751"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL2058018.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="PWY67_001752"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="PWY67_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL2058019.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="PWY67_001753"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="PWY67_001753"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL2058020.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="PWY67_001754"
     CDS             10630..10809
                     /locus_tag="PWY67_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058021.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="PWY67_001755"
     CDS             10831..11856
                     /locus_tag="PWY67_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL2058022.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="PWY67_001756"
     CDS             12031..13275
                     /locus_tag="PWY67_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL2058023.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="PWY67_001757"
     CDS             13428..14375
                     /locus_tag="PWY67_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2058024.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="PWY67_001758"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="PWY67_001758"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL2058025.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="PWY67_001759"
     CDS             complement(15277..15519)
                     /locus_tag="PWY67_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058026.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="PWY67_001760"
     tRNA            15640..15712
                     /locus_tag="PWY67_001760"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="PWY67_001761"
     CDS             complement(15831..16469)
                     /locus_tag="PWY67_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2058027.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5622 a         2947 c         3315 g         4606 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca
//
LOCUS       DANKAZ010000055        16406 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000055 DANKAZ010000000
VERSION     DANKAZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..1141
                     /locus_tag="PWY67_001762"
     CDS             <1..1141
                     /locus_tag="PWY67_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2058028.1"
                     /translation="RNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVLDFVARTMSTLE
                     VKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIFTDDNQLLIADD
                     FSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTRGLFEDYAALFG
                     RILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIWNSFKTNSVAIV
                     PKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALIYGEKAELDSNI
                     KAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLEYATQIDKITSG
                     GAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1128..1832
                     /locus_tag="PWY67_001763"
     CDS             1128..1832
                     /locus_tag="PWY67_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2058029.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            1837..2997
                     /locus_tag="PWY67_001764"
     CDS             1837..2997
                     /locus_tag="PWY67_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2058030.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3073..3351
                     /locus_tag="PWY67_001765"
     CDS             3073..3351
                     /locus_tag="PWY67_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2058031.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3332..3703
                     /locus_tag="PWY67_001766"
     CDS             3332..3703
                     /locus_tag="PWY67_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDL2058032.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            3719..4123
                     /locus_tag="PWY67_001767"
     CDS             3719..4123
                     /locus_tag="PWY67_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058033.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            4120..4530
                     /locus_tag="PWY67_001768"
     CDS             4120..4530
                     /locus_tag="PWY67_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058034.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            4591..5178
                     /locus_tag="PWY67_001769"
     CDS             4591..5178
                     /locus_tag="PWY67_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2058035.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            5251..5718
                     /locus_tag="PWY67_001770"
     CDS             5251..5718
                     /locus_tag="PWY67_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDL2058036.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            5764..6072
                     /locus_tag="PWY67_001771"
     CDS             5764..6072
                     /locus_tag="PWY67_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058037.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6267..13001
                     /locus_tag="PWY67_001772"
     CDS             6267..13001
                     /locus_tag="PWY67_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2058038.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            13005..13877
                     /locus_tag="PWY67_001773"
     CDS             13005..13877
                     /locus_tag="PWY67_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HDL2058039.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            13874..14923
                     /locus_tag="PWY67_001774"
     CDS             13874..14923
                     /locus_tag="PWY67_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058040.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            14927..15751
                     /locus_tag="PWY67_001775"
     CDS             14927..15751
                     /locus_tag="PWY67_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058041.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            15769..>16406
                     /locus_tag="PWY67_001776"
     CDS             15769..>16406
                     /locus_tag="PWY67_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDL2058042.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         5866 a         2530 c         3510 g         4500 t
ORIGIN      
        1 aagaaatgaa gagccttctg agtggatacc ggattttgta tatggagagg aagtatctgc
       61 tcgggcatac ctcaaaatta tggcaaaaaa tactgtttta gattttgttg caagaactat
      121 gtcaacgtta gaagttaagt ttaaaaataa agatgggaca tcagactggg attatattct
      181 aaatgttcga cctaattctg atatgtcagc agctacattt tggcaaaaat ttttttatcg
      241 gctcttagat gacaacgaag tgttagtaat atttacagat gataatcaat tgctgattgc
      301 tgatgatttt agtcgaacgg agtatgccgt gtatgatgat gtttttacaa atgtgatggt
      361 aaaaaatttt gtctttgaac gttcatggaa catgtctgac gtcatttata tggaatataa
      421 taacgaagaa ttagaaaggt ttacacgagg tttatttgaa gattacgccg cgttgttcgg
      481 acggattcta gaagttgcaa tgcgtaacaa ccaaattcgt gggtctgtat ccattgattc
      541 aacgggaacg gcgaatgatg aaaaaggaaa agatggtaag actagatctc aaagattaca
      601 agaatatatt gataggattt ggaactcttt taaaactaac tcagttgcaa ttgttcccaa
      661 agtgaaaggc tttgagtatg aggaatacac caataaacaa ggcgtatcga atcaatcgct
      721 agatgaactg aataagatga agtcgtcttt aattgatgat gttgccaacg ccataggagt
      781 acctacggcg cttatttatg gtgaaaaagc tgaactggat tcaaatatta aagcttttcg
      841 taaattgtgt attgctccac tagttaaaaa acttcaagac gaattggtgg caaaaataat
      901 tagtcgtcaa gagtacaaaa acggggaacg tatagaagtt aaaaaagttc tccaaaaaga
      961 tctattagag tatgctactc aaattgataa aataacatct ggtggtgcgt tttatattga
     1021 cgaagttcgt gatgaagttg attatgatga gttgccaaat aatgaaggaa aaattcgtat
     1081 tcgtacgaaa aactatgaag aaactgtgaa gggaggtgag aacgacaatg ccgaaagcta
     1141 aaaaagtacc atttcagttt actaacgaaa ttcagaatgg taagcacatt ctcaccttga
     1201 gtggaaatgt acagaaaaaa tattggcgtg atgatgatgt cataaacgct aaagatattc
     1261 gtgactctct agatactgta accgatgata ttgtaatcaa gttgaacagc ccaggcggtg
     1321 atgtgtttga agggattgag atttacaatt atttaaaaga tcacccatca aatattactg
     1381 tagaagttac agggcttgcc gcaagcgcag caacgtttat tgtcgctggt gccaatgatg
     1441 tgattatgaa tgttgggact tctttgatga ttcatgaagc ttctacattt gcttggggga
     1501 ataaacagga catccaaaaa acgttaaacg cattggaaac gatcgatgat tctatcttag
     1561 ctatttattc agataagact ggccaatctg ttgataagtt gcgtgaatgg atgaatgaag
     1621 aaaaatggtt tacagcagat gaagctgtca aatatggatt tgccaattct gtgaagcgtg
     1681 aagaaccaca aaatgaacct caaaatattg cagcgatgat tcaagatgct gttgccgttg
     1741 caatagcgaa tttaaaggaa cctgttacta ataaaatcga acaagaatca aaacaaaaat
     1801 cattaatcgc acgattgcga aaaggagaat aaagatatgt taaaaattac agataaaact
     1861 gcagatgcaa aaaaggcatt taatgctatt tcttcgaaag aagatgcaac acccgaacaa
     1921 attaatgctg ctctagaaga atatgtaacc gccattgcag aagatgccgg caaacaggtg
     1981 cgtgctgaat atgaagaatt gaaaaatgtg acggacaacc gtgtattaga ggctcgtgga
     2041 attcctacac taacagcaga agaaacaaag ttttataatg aagccgtaaa atctggaggt
     2101 ttcgattctg acgtagtttg gccagaaacg attttggaac gtgtttttga aaatcttcaa
     2161 aacgatcatc ctattttagg cctcattaac tttacaccaa ctgtaggtcg tgtcaaagta
     2221 attagagcac gtcgtaaagg tgttgctgtt tttggaccat tgcataaaga ccttgaaggc
     2281 caactagatg ctgaatttgg tgcaactgaa tttgttcaac ttgcgttgac cgcatttttc
     2341 ttaatttcta atgatacctt agatttagga ccacgatgga ttgaccgttt tatcaatctt
     2401 tctttaagtg aagcagtacg tgatgtttgg gctaaaaaaa ttattacagg ttctggtaaa
     2461 gatgagccaa ttggattgct taaagatcta gatggtgcgg tcacaagtgg tgtatatcca
     2521 gataaagcta gcgcagggac attaactttt gcgaaagaga agattgttct tgaacttgca
     2581 ggggtgatga aaaaattatc taaatacaca tacaaagtag atggtgacga tgaaggtgaa
     2641 gtgaaatacc gttctgttgc tggacgagtg tatctaattg ttaatcctgt tgattactgg
     2701 gatatcgtag ctagagttac atttgcaaat ttaaacaatg tgtatgggac tacgatgcca
     2761 tttatcgctg ttgatcatat catcgaatct gtagacgtac cagaaagtaa atgtattgca
     2821 tacgttgata aagagtatga agctactcaa tcaagagctg aaaaagtcta tgtatataaa
     2881 gaaacttttg caatgaaacg cgcaactttg tatgctgtcg acatgcttgg taatggctat
     2941 ccaacaaata atgatgcagc acaagtatat gacttggact ttacagcggg ggagtaatgt
     3001 cccctgcaat tggtgagata acaccgacga ctgatggggc agtcattaat ctttcgtaaa
     3061 tggggttaga gtatgaacac tgaagaattt ataaaagaat ataaggcccg atttcgtatt
     3121 ttccatactt ccgaagatga ggatatcggg aaacagttag agagcgggtt tgccgatata
     3181 aaatctctaa tcggcagttt tgatccttcc acttatgaga aaggaaaaga attagtttat
     3241 gaacgtactc ggtatctacg gaacgaatca ctagagtact tttacgacaa ttttcaaaca
     3301 atgattatgg atgcatcaat tgatctggca ggtgctggat atgccaatta aatcaaatta
     3361 taagaagcct aaaattgtag ctggtgatct aaatacgcca gttacttttt ttgagtttca
     3421 acctaataag ggtccagaac ctggtgatga agaaaaaaag gaactgtatt tttgtacagc
     3481 actagtttac aacccatcaa tgaaagatcg tgacattctt ggcacaaaag aaggcgttac
     3541 gataaaaata cgggatcccc atcaatctta tattcctact aacaaacata aagtaaggat
     3601 attagattat cgatatggag ataaagtttg ggaaataatc gatgtctcac cggatttgga
     3661 agaaaattca attgtaaaga tagtattaga attaattact taaatttggt gataaatgat
     3721 gagtaacgta aagtttgaag gcgttcaaga aacaatccaa gctttagaaa aaaagtatgg
     3781 agaaaagaaa actaagacat taacaaaaaa agcaatcaat gttggcgctg aaaaagtaga
     3841 aaaacagttg caaacggaca tgaccgtttt taaagacaaa ggttatacga tcgctgaggt
     3901 tgttcgcaag aacgctacgt atagagatta caaggcagaa gctgaaatcg gttggaatgg
     3961 tccacatcaa cgatatagaa tcattcattt gaatgaatgg ggatatacac gaaagggcaa
     4021 acaaattaga ccgagaggat ttggtgtaat aacaaaatct cttaagaatt ccgagctgtt
     4081 gtactttgat gctgtaggag aggaggttaa aaagagttta tgaaagatat gcttaatatc
     4141 atttacgagg ctctttgttt gaatgagtat attcattcaa tgacctacaa cgagaatacc
     4201 gagcaatatc gtattaaatt ttatgagcaa cctgaaaccg ccgataaaac aggtccaatg
     4261 atcacaattc gtcctgtaga tacacctaac gaagcgtacc acggaagtaa caaagagctt
     4321 tctgtggagc atatgataca gattgacgtt gaatcgaaat atagagcaac atgcaagcag
     4381 attcaatatg agataaaaaa agagatgaaa aaactaggct ttggccaaat ttccggtcaa
     4441 gggttagatg aatactttac tgaaacaaac cgctttgtag acgcgcgtcg ttacgaagga
     4501 aatacaaaaa tttacgatac cgaatattag aaattaagac acgaaaataa cgtgtctttt
     4561 tttattaccc aaaattagga ggaaaatttt atgactttag taggattcaa aaaaatgaca
     4621 atcggggtct ttgacagcac agggaagatt ccagcagcaa atttatatgt aattgaaggt
     4681 gaacaaaaca aaggggcgac tgtatctgct gaaatcagtg ggctatcaaa agaaccatca
     4741 aaagtttacg gatcgaacat cgcttactat gtctctcaaa aaggaacagg cgatgtttca
     4801 gctacttttg gattgttaga tttaccaaca gaagtaaatg acaaaattct tggttacaaa
     4861 accgatacaa acaaaatcag cttcttggga gaagatactg agccaccata ttgtgcaatt
     4921 ttgatggaat cggaggattt gaacggcgat acagcaatgc ttgcgatgtt caaaggaaaa
     4981 ttcagtcgtg aatctattaa tcttaacact acgacaaatg aagctttcga accagaagca
     5041 gaagaatatg tattttcagc gattgccaat gatgttgaag gtgatgctaa aggccaaaca
     5101 gtcgcaaaat atgttggaga cgaagaagct tctattactg gtttgaaaga gatggtattt
     5161 cctgcagcgg gggagtaact agccccgttg ttggagcaat cactcccact attaatgggg
     5221 caacagttga attaagttag gaggataact atgccagata catttaaaat ctataaaaaa
     5281 aatggtactg aatttacaaa agtcgcagag ggtgaaagtc cactttctat tactggaatt
     5341 gcagcaaata ctcaagttgc taaaggagat tatcaagcta cgcgtgtggt cggaggtcaa
     5401 gaatcagtaa aagtagatat tcctgcattc accactcttc caatttcagt tactggtgtg
     5461 acgttggata aaacgacagc tgaagttgaa caaggcggaa ccttaaaatt aactcctact
     5521 gtaacaccag caaatgcaac agacaaaaca ggaagctgga gcagttcgaa tacagctgta
     5581 gctacggtta ctggaggcaa cgttacagta aaaacagacg ctgaagttgg cggaactaca
     5641 gaagtttcat ttactactac ggatggcggt aaagtagcaa aatgtactat tacagttatt
     5701 gaaaaagcag cgggctagtc ttcggactag cttttttatt tggaaaataa tggaggaatc
     5761 taaatggcaa aagtaagaat tgaattgaaa gaagaatcag gaaagaaagt ctatgaaaac
     5821 atagacacaa cagggaaaga ttatcgaaaa gctttagaaa caatcaaaaa gctaaatgaa
     5881 gacggagtaa tgatttggaa tcgtttagac atttatttgg attttgctgt aggtatcttc
     5941 agggatagca aattgacagc tgaacaaatt cttgatggct taccatctga gaaggtaatg
     6001 agcacattgg acgacatcct aggagaagta atggggattg aaagtaatcc agacccagac
     6061 gcaaaaaagt aacacccgaa gaagcagaag aaatgtactt aaacctatgc agagagttag
     6121 tgaaagcggg atggtcacta tctgacatag aagataattc ctttgaaatg atgatgaaaa
     6181 ttgcgtgcac ttcgcctaag aaagagaaac aaaaagaagt tgatcttaaa gacttcttga
     6241 aatccatgta gagaggaggt aaacatatgg caaatggaaa acctttaggc aatatgatta
     6301 tcaaactaga cttagacagt tcagcttttt caaaaggtct agccggagct aaaaacgctg
     6361 ttaaccatca aatgaaagca atgaaatcac agatgcaagt aatgaactct tcaggaaatt
     6421 ctctcggtgc tttacaagca aaatacaatg gtttaggcgg agttctacaa gcaaatgaaa
     6481 agcaagtaga attgctcact aaggcttata aggatagttt tgactctaac ggaaacgcta
     6541 ctgcttcgac tgctaaatac gctaatcaat tgaatcaagc aacagctaga tcagcaagtt
     6601 acgaatctca gatgaaaaca actgttggac agattgctcg gatgaaagtg gaaacggaag
     6661 ggtttacggg gaaactaaag gctaattctg atgtattgat taattctggg aaaaaaattg
     6721 aagcctttgg taaaggagtt tctgggatag gatctgccct aactgttgga gtaacagctc
     6781 caatagtggc aggcgcaact gctgttacta gtgcagctat ttcatgggaa agtgcttttg
     6841 caggtgtaaa aaagacaaat gatgaagttg tagactcgaa tggaaacgta gtttattcct
     6901 atagcgactt agagaaaggt ctgcgtaatc ttgccaccca attacctggt agccatcaag
     6961 aaatagcaaa tgtagccgaa gcagccgggc aattaggtat aaagactgaa aacgtagtta
     7021 gcttcactaa aacgatgata gacatgggtg aatcgactaa tatgtcggct gaaactgcgg
     7081 caacttcttt agcacgattt gctaatatta cccaaatgtc tcaaaaagac tttgataaat
     7141 taggatcagt cattgttgat ctaggtaaca attttgctac aaccgaatca gaaatcactg
     7201 aaatgggatt gcggttagcg ggagctggta aacagattgg aatgagccaa ggcgaaattc
     7261 taggatttgc tactgctcta tcttctgttg gtgtcgaagc tgaagcgggt gggtctgcct
     7321 tttcgaaggt catgatccag atgcaactag ctgtagaaaa aggatttggc gcttttgatc
     7381 agctgaaaca gatggcagaa gaacaaggtg ttccgtgggt taatttagta aatgctgtga
     7441 gagacggtgg aaaatcgtta aaagcagtat cagagcaaat gggattcact tcatctgatt
     7501 taaagaaaat gtataaagaa gcggacaatt cgaaaagttc gcttgaaaat tttgcgaatg
     7561 tggcaggtgt tacaagtgaa caattccaga agttgttcaa gagtgatcct tcagaagcaa
     7621 tcatcaagtt cattcaaggg ctgaaagatt cagaaaaaca tggtacttca gcaatcaaag
     7681 ttctggatga catggatata aaagaagttc gactacgcga cagtttattg agagctgcaa
     7741 atgctagtga catattcaaa ggagcaattg aaaaaggaaa cggcgcgtgg aaacaaaata
     7801 cagctttaac aaatgaagct aataaaagat acgaaacaac tgaatcaaag ctaaagatat
     7861 tgaaaaacga agtgattgat acagcaatcg atcttggagg tccatttgta gacgcgttgc
     7921 gtgatggtct aaatgtatcg aaaccattga taaaaaactt agggaatatg gcgaaagctt
     7981 tctcagctat gagtgatgat caacaaagaa acattatcaa atgggttgga ttagcagcag
     8041 cagctggtcc agcgctgaag cttttaggaa ctggtacttc agtaataggt aaagttactt
     8101 caactactgg aagattgacg aaaagccttg ttgatttagc agcgaaagca gcagaaaaga
     8161 aggctatgga tgcctttgca tcgtcggtaa caacagctgg tgctgcagct gcaaagacag
     8221 caggagctgg aggtattggc gggttagtcc ctatgatggg aaaaacagct agtgctgcaa
     8281 gtactgctgc gggcgccagc ggaataggcg caatgacaac gtcactaggt ttgttaggtc
     8341 ctgctctact tggtattgtt ggcgttggtg gcgcactggc agttggctat ggagcatgga
     8401 aattatttgg tgaagaagct tggaattcct cgcaaagagt aaagcaatgg ggaacagatg
     8461 tcggtgagga gatagatagt actcttgacg gagtacagaa gaatattgaa ggtgcaaatg
     8521 gtcaattcgg tcttctcaaa gagggtttta ctgttgatga tgcaaataat atggcggcta
     8581 atttcgcaac cgctggaaca tctttggaaa catctttaac gggtagaata aaggcattag
     8641 acgatgcact aaaagggcta cctcaatcag ttcaggatgc catgaaagaa gtagctgaat
     8701 cagaagaaga aaccttaggg caatctcttt ctaaaataaa agacaacaat gaagaaataa
     8761 aaaaaatcag agaaagagcc gcaaatcaga acagagactt gactgtttca gaactacaac
     8821 agatacagga tctaatgaaa ggaacttctc aagcgtatgt ggatacgctc gatgtttctg
     8881 ctaaagacag aaaaaagata ctatcagcca tgaatggtga tgttgcacaa gctacagaag
     8941 aagaagcgaa gacgtggtta caatctttag cgaaacaaaa agctgcaact accgaaaatt
     9001 atagacaaat gcttgaagaa aaaaaggcgg ctttaaagga aaagggagta agcaaagaat
     9061 tcattgatgc attagaagat gaaatgaatg attacatcaa aactacaaat caaggattcg
     9121 atagccaaat agctgctata acaaataaat atccacaatt gattaacgaa gtgagtttat
     9181 caaatggtca attatttagc aaaacggcag ctgctatgga caatactggt cagtatacaa
     9241 aagtttttct cgaaagtaat gaaaagattt tagcccaagc gaagaaaaca tcggatgaaa
     9301 tagcagataa tgccgaaaaa aatgcagaac aacttggtat ggtagctgat gaggctacgc
     9361 atgctggatc aacttggaat ggcttagttt tagacgaaaa aacaggaaaa gtaaaaacta
     9421 atgtacaaga agtagtgaat gaagcaacga aggatttcac taaatggaat gaattgaaac
     9481 ctgttgttca tgatgcgaaa ctaacaacga atgcaaaaga tgtcatttcc atagcggcag
     9541 tacaaaatgg ctattgggat gatatggaat ggaaagaaaa agaagcatta ttagaagatg
     9601 agtgttctaa aaatgtatac aaggctttag aaaatgctgg taaatggaat gagttagata
     9661 ttccttctaa agaagcaatc ctaacttcaa atactcctga agttatgggt gaaactctca
     9721 tgaatttggg attatgggat acttacaaac ttcaagtgaa agatttagat ttgaatgcgt
     9781 ataaagtata cgatgctatc gatggatcag aagaaaaatt aaatagttgg gctcaattag
     9841 ataaccccac aaaagaattg ttattagata actcagattt ttctacaaaa ctttttaatt
     9901 ccgaagaaat gtttaaacgt tggaatcagt tatcatctaa tgcaaagttt ctttcggcta
     9961 ataatactaa atttacagag gttcttttat catctgacag aatgtggaat caatgggtaa
    10021 accttcctga taatgagaaa agattacttg cagataacga agatttagct accactgttt
    10081 tatcttcaga agaaatatac ggaagatgga ctcaattacc agataatcaa aaatatatgt
    10141 tagctgacaa tactgactta gcaaacaaaa tattcgcttc agaagaatca tggaatgcat
    10201 ggaaagcaat tccagacaca gttaaaaact tgcttggtaa taatattgat ttactggcta
    10261 aagtgaaaga cgggactgta agtgtagaag aatataacaa agttatgcca cttcttaaaa
    10321 ctctatatgg agataacgtt aatttaaaag aggcaatcag tggggctaaa agctctatag
    10381 agaattacaa caaaaatatt tttcctagtg ataaaactgc agtaggacac gatgaggctt
    10441 cacaagctgc taaagatgca tttgaagcgt tcaacatctt ccaaacaaaa ataccagata
    10501 aaattactaa aactgtatca gctgacttta taggtcctat gccgaaaaat gcgaaaggaa
    10561 caaacttcca tcctggcgga gcagctatgg taaacgatca aaaagggcct acttatgaag
    10621 agcttataac cttgccaagc ggagaaactt ttatacctaa aggacgtaat gtcgttttag
    10681 acttaccaag aggttcgaaa gtactgaacg ccactaaaac taaacgtcta gtgcctaaat
    10741 atgctgatgg tataggaaac ataacgacgg tttcatctac gctaaactta gatgctttaa
    10801 ttttagctat taacgagcta acgacggtat tgagaactca actgatggca ggaacgactg
    10861 atgctaaaga tactaaacta tcagatgaaa caaaagttgc cgatccgatt attccggaca
    10921 gtctttctga aaaatcagat cagtatctag gaattggagc tcaatggtta actaatctaa
    10981 tgaatggttg gaactcagcg gttcctcaat atatgaatag tgaaatggtt tttattacaa
    11041 actatctcaa tgcgctaaaa cttcaaaaca atcctaatta tacgcaagga gcaacatgga
    11101 ataagaactt actgaatgga tggaatagtt taactgggac ctttattgct acaatcaatt
    11161 cattttgtaa tcaagcaatg gtcacgctta gaaactacaa cactcctatg tacaacaatg
    11221 gccgaacgtg gcagcaaaat aatcttaatg gttggaattc attgtatgga tcattcatag
    11281 cccgtgtaaa tcagcttggt aacgattcga ttaacaacct tcgttctaaa agtggtggtt
    11341 tttacaatgc agggacattc ttgttgcaat ctttaataaa cggaatgaat tcgttaggta
    11401 attctctttc tactacaatg aacaatgtcg ctaatacaat ggttggtggt ataggcaaag
    11461 gagttaatgg cgtcatatcg ggagttaact atgtgcttaa ggaagtcgaa tcaagtaaga
    11521 atatcggaaa ctggccaatt ccacaatacg ctaagggaac tgatggacat cctggtggac
    11581 ttgctatgat taatgatcaa aaaggcctag ttcacgaaga atatgttcag atgccagatg
    11641 gacgtagttt tattgccaaa ggtagagatc ttcttgttaa tttgcctaaa ggtgctcagg
    11701 tcttaaatgc ttctttaaca aagaaattaa aggaacgttt aaatattcca cactacgaaa
    11761 aaggtattgg caatttagat attgttgatt tgttagatga tgaaaaaaga atgttagaat
    11821 tcttaactag caaagttgat ttttcaggca ttaatgaacc atggcttgat atgactaaat
    11881 caggaacgtc attaatgtcc aaagctgcaa atacaatgct tcaatcaaaa ttaagtgaat
    11941 tcttcactca tggcaatttt gatggtgcag taaatgccaa tggtgtttat caatatttag
    12001 ttgatgtggc acagaaagta atgggtaagt tccccggact tacagtaact tctggatatc
    12061 gagcaggaga tgcctactat cacggaaaac ggcaagctat cgacttggct tatccaggta
    12121 tttctggaga tccgagatat acagcagcag caaattacgc ttttgaaaaa ttcccttcaa
    12181 aaattgcgta tgtcattacg aatggacgtg tacgtgaccg catgggatta tctggaacaa
    12241 ggtcaagcgg acaatggacg aattggccag atggcgatca cttcgatcat attcatttaa
    12301 acggttcgat ggggtctggc gatatattca atggaggagt tggaggaagt ggtgttgcac
    12361 gctggcgttc ttatgtgtct aaagcattga aaatgaatgg attaccagct acagcagcgt
    12421 atataaatgc ctggatgtcc caaatccaaa cggaatctgg tggtaatgag aaagcaatcg
    12481 gcggcaatga tgggctcgcc gaaggcaatg caacaggatt acttcaaacg aaacccggaa
    12541 cttttgcagc taacgctttt cctggtcacg gaaatattat gaacggattt gataacatgt
    12601 tagcagcaat caattatgct aagaaacgtt acggagttgc aggtatgttg caagttatcg
    12661 ggaaaggaca tggatatgcc aatggcggct tgattaccaa agacggacta tatcgagcag
    12721 gagaaggaaa taaaccagaa atggttattc cattgacaag aaaaacaaga gctatcgaac
    12781 ttatgggtca agcattagcc ttcctttctg gaagcaataa aaaagaaacg aaaagtgaga
    12841 ttaattctgt agataataga catatcctac aagcgataga aaaacagtct cgtatgacaa
    12901 atgatctgct atcattatta ctctcgtttt ttaaaagtaa caatcgatca gataaagaac
    12961 tagctttaga tattcagaaa attttaatca ggaggacata gtatttgagg acagtaatat
    13021 taagaaataa attgaatgaa gaaattgatt tgtccacaga agattatttt gctactgaaa
    13081 tgtcgaatct aggttttgaa gttacaaaag agcatattgg acaatggggg agtttcagag
    13141 aaataggaga gagtattgaa ttgtctgaat ttcaatcatc agttatgatt tctgttcacg
    13201 gatttagaga acaacaatta tatggttcat tagttgaatt tctggctaat ggaccttttg
    13261 aactagaatt ttcttttgat tcagaaacaa tgatcaggaa atgtagtttg aaatcattat
    13321 ctaaaacaga aattgattcc caaagctcat tgctaacaga tactttgaat ctatatttta
    13381 cttcaagctg gtattctatt aagcgtgaaa aattaataca gaattcaact tctctcaata
    13441 cacgtggaaa agtttactca tttgttcgac catatgtcta caccaaaaat gtatgggaaa
    13501 agaaaggtgt atttaagttc agtaataatt ctgtatattt gacaaatagt acagagcgaa
    13561 tgtctccgtt aaaaataagg attattggag agtgctcaaa tccatattgg gaagttatcc
    13621 aaaactctaa agtagtagct tcagatggct attttattaa tatgaaagac acacaaatat
    13681 tagaagtgtc tagtttgcta gaagaccaaa cggcaatttt aaaagatgta ggtggagttg
    13741 aatcttctgt ttatcaacaa caagatttta ctaaatcaaa cttcgtccaa gcgcctctag
    13801 gagaattcag cgttgttttt catgttggac atgcaaatgt agagattgaa ctttatgagg
    13861 agcgtgatct attttgattt tagccattac cctctttcac cgagatatga atttatataa
    13921 cgaacgttta atttcgactg agttcgaatt tggaacggat gaaattggag aagaagtaag
    13981 taattttatt acagataaat atgtccatgt aaaatcagga gattttctac tagcaaaatt
    14041 tattcctagc gggaagattg cttattttgg agtgattagc tcgcaagaag acaaaaaaat
    14101 aaattgcaaa ggattactca gtttggccga tagcgagata ccgactgtaa aaatatcagg
    14161 tgctaattat gaagagcata ttcgacgact gattgaatac tacttattga acgatccaac
    14221 aaaacaacta aaaaatattt tggaggtaaa aacagaaagc gttacttctc attcttatca
    14281 agctgttgac acaaataaga gacgattgaa tgcatatata ataaatgggt tcaaaaaata
    14341 taatgttaaa tggtatgtca aagagattaa aggtaaaaaa atatatactg gtattcgagc
    14401 agttgatgat tcaatttata ttaaagataa ttcatctgag tttaatgact gggatgtttt
    14461 tgtgcaatct cctggaccag ggaacgagaa caaattattg atcgtagata aagcaatgaa
    14521 agatatagaa aatcccttgg tattgtctac gtggtattta gatgacgaaa ataatcttac
    14581 gcaagattca acgaataaga acattgcatt gcctaccgtt aatttagtaa atatatatga
    14641 tcaaactcaa gaagatagac cttcttatga agaggtagct aaatcggagc ttaaaggcaa
    14701 cgcctattca catgaaatta aagttaatgt tgtgctaaat gctagaaata tcaatgtaga
    14761 aaatatcgaa acaggaatgt tagcgactat tgtttacaaa gagcagactt acaaatctgt
    14821 tctaactgcc tggcgaatat ccagcagtaa aaagaatata gaattgacat ttggtaatgt
    14881 ccgaagtcgt tttatggatt attttgaaga taatggggga taaaaaatgg ttagcaatgt
    14941 ggatggatat caatttgaaa acgtgaaagt aagcgcagaa aatgatgcta gactttatca
    15001 cgttttatat aatcggaaaa atcaggttat tgatggttac gatcagtcta tgaatttatc
    15061 ttcaagcgga ttaacagtaa aagttgctgc aggagcagcg attattcaag gtcgtatggt
    15121 cgttgttcga caagaagaaa gtataacagt tccagcaaac tcaagtggtt atatagcatt
    15181 aacagtggat ttgacacaag aagttatacc tggatctatt cttccagaat cggaagaata
    15241 tgaatggact aataatcaag tcaagctaga atttataaca aaagttataa aaggtaattt
    15301 aaacaatggt gataaggtct ataatttacc actatgctca gttaattcta ctggatcaac
    15361 tgtttcaatc tcaaagatat cggatagtta cgagctgact ctctctaaag gagaaatttt
    15421 gtggaggggg actgcgttaa tgcatgatac tcaaactgtc caaccttcaa aaaaaatttg
    15481 gcagacagtt tcaggttttt tgttagtatg gctcccatac gaaaacgggc aagcaattga
    15541 ggatagatat gtaactacgc ctttttataa ggagcgtata acttttacta atgttttagg
    15601 agaaattgtt tcaggatttg atgactatca caaaaagtgg tttagtaaac gaataaacta
    15661 taattcgaat actaatatat ttacaggtgc agcaagcaat gcaagtggag ataacgcaaa
    15721 tatggtgctt aggtatatag tttcttttta gttaggaggt atggaagagt ggcaaattta
    15781 gaaattaaat tatctgcaaa taagagacag ccttatctac gtcaccgtgt tgttggtaga
    15841 gttggtgatg gggggctcac aacaatcaat gtacaacttc ttgaggaaga tgaaattaca
    15901 ccttttgtaa ttaatctaaa cggtactttg aaatttgtgg gcgaagtttc aaacggtaac
    15961 tataccgagg gagaaccaga aataatcgat tcgactaatg ggttaattag ttacacgttc
    16021 actaagtcaa atttcagcac gagtcatcaa ttcaaacaag catattttga atatgtagat
    16081 cctaacggca aaaaagtaac ttttcagaac ttcatcatag acgtgttaga acgagtagat
    16141 attaattcgg agcaagcgaa atactatatt tcttcattgg aaaaattaca gagtgaaatg
    16201 caaaccactt tcaatcagtt cattagtgat aaacaggtcc aatacgatca aatctactcg
    16261 aaatataacg aattagtaag attgataaat gaatcagata agcaagtaaa tgatcgtatt
    16321 gataaggcaa ataatcgtat tgatcaaacc aatcagcaaa tcggcgatct cggcaagctg
    16381 aaaaagatgt acagtaacag cattga
//
LOCUS       DANKAZ010000056        16335 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000056 DANKAZ010000000
VERSION     DANKAZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="PWY67_001777"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="PWY67_001777"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL2058043.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="PWY67_001778"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="PWY67_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL2058044.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="PWY67_001779"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="PWY67_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL2058045.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="PWY67_001780"
     CDS             2857..3732
                     /locus_tag="PWY67_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL2058046.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="PWY67_001781"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="PWY67_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL2058047.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="PWY67_001782"
     CDS             4629..5063
                     /locus_tag="PWY67_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL2058048.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="PWY67_001783"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="PWY67_001783"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL2058049.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="PWY67_001784"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="PWY67_001784"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL2058050.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="PWY67_001785"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="PWY67_001785"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL2058051.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="PWY67_001786"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="PWY67_001786"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL2058052.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="PWY67_001787"
     CDS             13334..14260
                     /locus_tag="PWY67_001787"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL2058053.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="PWY67_001788"
     CDS             14442..15335
                     /locus_tag="PWY67_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL2058054.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="PWY67_001789"
     CDS             15469..15594
                     /locus_tag="PWY67_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2058055.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..>16335)
                     /locus_tag="PWY67_001790"
     CDS             complement(15728..>16335)
                     /locus_tag="PWY67_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058056.1"
                     /translation="FLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWLLLDHSDITE
                     AEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAILFYSFN
                     HNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFVLALM
                     PLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5804 a         2784 c         3487 g         4260 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaat
//
LOCUS       DANKAZ010000057        16215 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000057 DANKAZ010000000
VERSION     DANKAZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            115..1206
                     /gene="phnW"
                     /locus_tag="PWY67_001791"
     CDS             115..1206
                     /gene="phnW"
                     /locus_tag="PWY67_001791"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDL2058057.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1199..1327
                     /locus_tag="PWY67_001792"
     CDS             1199..1327
                     /locus_tag="PWY67_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2058058.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            1331..1876
                     /locus_tag="PWY67_001793"
     CDS             1331..1876
                     /locus_tag="PWY67_001793"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2058059.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(2330..4021)
                     /locus_tag="PWY67_001794"
     CDS             complement(2330..4021)
                     /locus_tag="PWY67_001794"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL2058060.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(4441..5388)
                     /gene="arcC"
                     /locus_tag="PWY67_001795"
     CDS             complement(4441..5388)
                     /gene="arcC"
                     /locus_tag="PWY67_001795"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL2058061.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(5503..6522)
                     /gene="argF"
                     /locus_tag="PWY67_001796"
     CDS             complement(5503..6522)
                     /gene="argF"
                     /locus_tag="PWY67_001796"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL2058062.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(6613..7842)
                     /gene="arcA"
                     /locus_tag="PWY67_001797"
     CDS             complement(6613..7842)
                     /gene="arcA"
                     /locus_tag="PWY67_001797"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL2058063.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            8303..9004
                     /locus_tag="PWY67_001798"
     CDS             8303..9004
                     /locus_tag="PWY67_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2058064.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(9613..10629)
                     /gene="tsaD"
                     /locus_tag="PWY67_001799"
     CDS             complement(9613..10629)
                     /gene="tsaD"
                     /locus_tag="PWY67_001799"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL2058065.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(10626..11090)
                     /gene="rimI"
                     /locus_tag="PWY67_001800"
     CDS             complement(10626..11090)
                     /gene="rimI"
                     /locus_tag="PWY67_001800"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2058066.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(11097..11639)
                     /gene="rimI"
                     /locus_tag="PWY67_001801"
     CDS             complement(11097..11639)
                     /gene="rimI"
                     /locus_tag="PWY67_001801"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2058067.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(11623..12345)
                     /gene="tsaB"
                     /locus_tag="PWY67_001802"
     CDS             complement(11623..12345)
                     /gene="tsaB"
                     /locus_tag="PWY67_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL2058068.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(12536..13516)
                     /locus_tag="PWY67_001803"
     CDS             complement(12536..13516)
                     /locus_tag="PWY67_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2058069.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(13540..15024)
                     /gene="galT"
                     /locus_tag="PWY67_001804"
     CDS             complement(13540..15024)
                     /gene="galT"
                     /locus_tag="PWY67_001804"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL2058070.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(15036..16025)
                     /gene="galE"
                     /locus_tag="PWY67_001805"
     CDS             complement(15036..16025)
                     /gene="galE"
                     /locus_tag="PWY67_001805"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL2058071.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         4622 a         3358 c         2896 g         5339 t
ORIGIN      
        1 ttcaaatttt tcaacacctt gattgaatca caataatcat catttatatt ggatgttaac
       61 aagagcagct tcttgctctt tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat
      121 aaattattaa cttctggacc attgacgact tctgaaacag tgaaaaatgc tatgctgatc
      181 gatcattgta cttgggatca agaatacaaa gaaattacac aatggataag aaaaaaatta
      241 ttggatcttg ctcaagtatc ggaaggaata tacacaacgg tcttaatgca aggtagcagc
      301 tccttcgggg tagaatcggt attaagttct gtgatcaggc cagaagatac tgtactgatt
      361 tgttcaaacg gcgcatatag acaacgaatg atcaacatgt gcgaatgtct gaaactgaac
      421 tataccgttt atgaggtatc ggaacatcaa atcctccaag ctcatgtgat tacacagctg
      481 attgaagaga atccgtctat ctccgcacta gcaatggtcc atagcgagac tacttccggt
      541 attttgaata atttggaaga gattgctgct gtcacacaag aaagagatct tttgtttata
      601 gtggacgcga tgtcaagctt tggtgggata ccgatttgtg tagccgaact agggattgat
      661 tttttgattt ccagctcaaa caaatgtatt caaggtgttc caggcttttc ttttgtactt
      721 gctaataaag cggaactgga aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta
      781 tatgaccagt accgcgtgat ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc
      841 catacggttt tggcttttca acaagcgctg aaggaactag aggcagaagg aggaatcgaa
      901 actcgatatt cacgttatct cgccaataat catgtgctac gacaaaaaat gacagagatg
      961 gggttccagc ctttcattca agaatatcag gggccattca tcacctcttt tctttaccct
     1021 aaggactggc gatttgattt taatcatttt tatcagtatt taaagcaaaa tggatttgtg
     1081 atctatcccg ggaaaatcag cgatcatgaa tgttttcgta tcggaaatat tggcgagatc
     1141 tatccggaag atattttgga actgacagca attatcaaag actataagga gaacgttaat
     1201 gattaaaact gtaatttttg attgggcagg aacaactgtg gactttggct gtatggctcc
     1261 tgtccacgct tttagaaatg cttttctaga aaaaggaatc caactgactg acaaagaaat
     1321 ccgctaaccg atgggaaaat taaaatggga tcatatccaa cagcttcttt ccctcccttc
     1381 tgtcaaaaaa caatggatag ctatttatgg acaactacct caggaaaaag aggtggagga
     1441 attgtatcaa caatttgaac attatctgtt taaagatttg gtcaagcatt ctaccttgaa
     1501 acccgacaca ttggaaacga tcgaacggtt gagaaacgct gggatcaata ttggttccac
     1561 gaatggattt aatgctccta tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta
     1621 ttcaccagac ttcatggcaa cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta
     1681 tatgatccat aaaaacatgc agtacttcaa aaacaagtcg gttgatgaag taatcaaagt
     1741 cggagatacg gtctcagata tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt
     1801 aattgaaggt agttcgctaa tggggatttc ttatgatgag taccaaaaat tagagatggg
     1861 gaaaaagatc gtttaaaaca gtctgttgct cttagattcc aacaagccgg cgctgatatt
     1921 tgtgtaaaca atttaaaaga gttaacagaa tacattttgt acgctgacat gaatccagaa
     1981 tgacttttat acaaaataag aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca
     2041 gccaagcaaa aatagctcct catatttttc gtttggttag gcttattacc gcagatgcaa
     2101 gcttttgaac accgtttatt cgaaaaaaaa aagggagctg tgacacgact tttgtcacag
     2161 tccccttatt tgcaaacaaa cagtgtttaa gagacaacct ctccactaaa cggtgttcat
     2221 tcagctgact ctcggtattt gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt
     2281 tgtttctcta atactcggat cagaacgctg atttcacatt ctctttttct tacatcttat
     2341 ctggtgcatg aagtcctagc aaacgaaggt cttctttcaa taatacagtc actgcataaa
     2401 ctagagctaa gcgtgcttct ttttgttcat catcagctaa aatcttcgta tgagcatagt
     2461 atttgttgaa tgcttgtgct aatttgattg catgtttcgc aatcactgac ggttcgtatt
     2521 tttccccagc agacaagacc gtttcagggt atttttgaac taatttaacc acttcccagc
     2581 tggtatcgtc attcaatgca tagtctgctg tttctgaagg gacaaatcct gctttttcca
     2641 acaggctgac tgcacgagca tgtgtatatt gcacataagg tccagtttct ccttcaaatc
     2701 gaacaacttc ttccaggtta aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga
     2761 aaatgactgc cccaacccca acttgtttag ctacagcatc tttgttttcc aaatcagggt
     2821 ttttctcgct gatttgttct ttagcagatt ggattgcttc atttaatact tcttctaata
     2881 agacgatctt tcctttacga gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg
     2941 gaatatggtg catgtcgtca gaccagtcaa agcccatttc cttcaagaca gctttcaact
     3001 gtttaaaatg atagctttgt tcattcccta ctacatatag agattgtttg aagtcgtaag
     3061 tacgtttacg gtacaatgca gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg
     3121 acttcttaat caacgctgga ttcaggtcat aagcagatag gtcaacgatc tctgcacctt
     3181 tatcctcgtt cagcaaatgt ttttcttcta aaagtttgac gatttcatcc atcttgtcat
     3241 tgtagaaagc ttcaccgttc aacgaatcga aacgtacttc cagcagatca tagattttat
     3301 tgaattcttt catcgattca tcacggaacc attgccataa ttgaatagcc tctttatcgc
     3361 cttcttccag acgtttgaac caagcacgag cttcttcatt taattctggt tctgtttcag
     3421 ctacttcatg gaattggacg tataaacgaa gcaattcatt gattggttct gctttaacag
     3481 cttcttctgt accccatttt ttgtaagcga cgatcagttt tccaaactgt gttccccagt
     3541 cacctaaatg gttgatgcgg attggctgat aaccgatttt ttccataatg aaaccgatcg
     3601 aattaccgat aactgtcgaa cgcaaatgtc ccattgagat tggtttggca atattaggag
     3661 aagacatgtc gattggtact gttccttgat tcccgatgtt gctatcccca taatgttctt
     3721 tttctttcac tactgtttgc aaaacttttt tgctgataag ttctttattc atgaaaaagt
     3781 tcaagtaagg tccgacaact tcgatttttt caaagttggc agaatcgatt ttttctgcta
     3841 aatctgcagc gatttgctgt ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa
     3901 atgcaacatc tccgtgttca gctgattttg gattttctag taattgctcg acttgttcca
     3961 gtgttaaatc atctttgaca gcgtcataga cagcttttgc gacaagatct ttattattca
     4021 tctttattcc tcctaaagtg ttcccttgat tgttaaacaa aaaaaatctc tagcgacggc
     4081 atacttagta tgccatcact agagacgaga tttcccgcgg taccactcca attgtcctaa
     4141 aaaaggacca gctccatctg atatcggtag acgaccgtct gtttaaaaca gaattctcca
     4201 agtgcgcttc gttgtatctt cttcattcgg cttccaccac ttccgaattc gctagccaag
     4261 aatgatacaa gtactctctt gttcaacaaa tcttagtctt attaaacttt cttgaattat
     4321 atcagaaaaa aaaacgaaag gctagtcgtt tcataaaaaa acaatccaga tggcctaaag
     4381 gagaagaaat accacctgga tagctataaa tcatttcgta aatacgaaag acttaacaaa
     4441 ttaatcagct acgataatcg ttccttcttc agaagctaaa agattctcga tattttctaa
     4501 agaagtgatg attgccttag cgtttggttt tgcttcaaca aatgcaatag ctgcttctac
     4561 tttcggaagc atgcttcctg gagcgaattg cttttcatca atgtattgtt tcatttctga
     4621 aacagtaaca gtttctaatt tcttttgatc tggtttttgg taattgacat acacattgtc
     4681 tactcctgtc aatacgatca gcaaatccgc atcgatgatt tcggctaatt tttctgaagc
     4741 aaaatcttta tcgattacgg cttctcgtcc ttctaatcca gtagctgttt ctacaacagg
     4801 aatcccaccg ccaccaacag agactgtgat aactccttga tcgaccagcg tttcgatgac
     4861 acgtgcttct ttaatcgaga tgggtttagg ggaagcgact acttttctcc atccacgtcc
     4921 agcatcttct ttgaatgtca cagtggagtc agcattcatt tgttctttgg cttcttcttc
     4981 cgtgtagaat ggaccgactg gtttacttgg atttttgaat gaaggatcat tttcatccac
     5041 aataacttga gtgactaaag aaaccacatc tttatcgatc cctttttcct tcagtacttc
     5101 gcccatggcg ttttgtaacc agtaaccgat agatccttct gtcattgcaa cacatgtatc
     5161 taaaggcatt gccggtgttt tctcagaatc agcagcttgc tgctgaatca ataggttacc
     5221 gacttgcgga ccatttccgt gggagatgat caattcatct ccttgttcga taaatttcac
     5281 taaatacttt gctgtttcca ttaacgcttc ttgttgggct tttgcacttg catccgtaga
     5341 caagatggca ttgcccccta aagcgacaac tacttttcgt ttggccattt cattcgtcct
     5401 ttcttcagtt ctaccgaatt ttctagataa caaaacgagc cagcgttttg tgattttccc
     5461 tcaatgctga ctcgtttttt cgtgagatga atgtggttat gcttaaacgc gagggataaa
     5521 caagttacct aatgttgctg ccatgatcgc tttgattgag tgcatacgat tttctgcttg
     5581 gtcaaattga cgagcatatt tgctgcggaa gacttcatct gttacttcca tttcagtgat
     5641 gccaaagcgt tctttcattt gttcaccgta aacagtgttt gtatcatgga aagctggcaa
     5701 gcagtgcaag aagatcaaac gatctgtgtt gtgtgttttt tctaccattt ccatattgat
     5761 ttggtaaggt ttcaacaatt tgatacgttc ttcgaattta tcttcttcac ccattgatac
     5821 ccatacatca gaataaagga cgtctgctcc gtctacacct ttatcaacat cgtcggtaat
     5881 catcaattgt gcaccggatt tttcagcaaa gccttctgcc attttgacga tttcttcttc
     5941 aggttgtaat tcttttggtg caacgatacg catattcgct cctaaaattg cacctgtgac
     6001 taataaagag ttagccatat tgtttcgacc atctccgcag taagcaacag tgataccttc
     6061 tactgttcca aagttttctt ggatagttaa aaagtcagcg atcatttgtg ttggatgcca
     6121 ttcatcagtc aagccattcc aaactggaac accagagaat tctgctagtt cctctaccat
     6181 tttttgactg aatccacgga attcgattcc gtcaaacata cgtcctagta ctttagctgt
     6241 atcttctgtg gattcttttt tacctaattg gatatcattt gcacctagat attcaggatg
     6301 tgcacctaga tcgatcgctg cagtagtaaa tgcagaacga gtacgtgtag atgttttttc
     6361 aaataaaagc gcaatatttt ttccttctag atagtgatgt gggataccac gttttttcaa
     6421 gtctttcaag tgttctgaaa aatcgatcaa atattgtaat tcttcttttg taaagtcttt
     6481 ttctgctaat aagcttcttc cttggaatac tgattctttc atattaaaca ttctccttta
     6541 gattgttttt atttaagatt aaagaggaaa tcccgacggc agctgacagt aagactgccg
     6601 tcaggaaaca aattacttca agtcttcacg gacaagtggt tggctcatgc agcgtggacc
     6661 accacgacct cgtgacaatt cacttgaatt gatttctaac actttgatgc catagctacg
     6721 aagcaattcg ttcgatacat aatttcggtt ataagtgact acaacacctg gagcgattgc
     6781 caatgtgttt gatccatcat tccattgttc tcgaggtgca acgatctcat ctccattacc
     6841 agttgggatc agtacaagat catctaatcc aagagcagct ttcatcgttt cgtgtaagtt
     6901 atctgaatga gtaatcttga tatcatcacc atctggttcg attgtaaaga catccatctt
     6961 accgtcttta ctttgaattg ctggatggat agtgaattta tcgtgatcta ccattgtaaa
     7021 tacggtatcc aaatgcatca ttgcacggtt gttcggaatt ttgattgcca acactttttc
     7081 aaatcctgaa ttttttgcga ataggtggcg tgctaatttt tctaaagctt tcgcattagt
     7141 tcgttgagaa atcccaacag ctactacttt atcgcttaag atcaattcgt ctccgccttc
     7201 gatatgatct ggattttcac gatccaacca tacttcgatt cctttatttg caaatcttgg
     7261 atgatgtttc aaaatatatt ctgtaaacat cgattcacga cgacgtgctt caaaagtcat
     7321 cttattgact gtcatcccat ttcccattga tgcggaaggg tcgcgtgtaa agtacaagtt
     7381 tggcattggg tccatgtaaa atggatagtc atcgtctgct gacagatcat atagactgct
     7441 agaacgtact tcgatatctt ttgtacgaac cccagccatg attttatcta ccatttcttg
     7501 cgtttccatt gatagaagga actcatgaag accctctctt acagcattcg atgcaatatg
     7561 agattcatcc aacattttat tcaagaattg ttctttgaca ttgcctgcgt caatcgcttc
     7621 tgccgctaat ttttccaagt aaagagtctc gacgccttca ttttgcaatg tcttcgcaaa
     7681 gttgtcatgt tcttcttgag caatcggaag atatggaata tcatcgaaca acagacgatc
     7741 cataatatct ggtgtcaaat tttccacttc ttgtccaggc cgtttcagta aaactgtctt
     7801 taatttccct atttcagaga aaacgtgaat aggtttatcc atgttgtgtt gcctccttct
     7861 ttttgattac atgtatagga tatcctcctt ttgtaatcac tttcataatt ttgtctcatt
     7921 caaaatgaga cattcctcac attaatattc gataattaaa taattaaacg tttttctatt
     7981 tttaagcgta atttattcat tttttattct gtatacactt tcaaaaaaat atttattcat
     8041 tgaaaaaatt tttttgaata aattaaaaaa ataaattata ttatcgttgt tagcgtttac
     8101 tatatgtatt attgttgttt tttatacaca aatcattcgc ttcaaaacaa attattcaat
     8161 accattgtat attttttaca taataacaaa ttatatttgt aaagtattaa aacttgtttc
     8221 taatgttggt atgttaaaac tttacaaatt ctttaaaaaa agtgacaata aatatagtga
     8281 acaataagga gaggactttt taatgcgtgt taataaaatc gattttcact tttcaaaact
     8341 cagatatcaa cctgattttg aacatttctc ggatgaagag ttccaacaga tcaaggaaca
     8401 tactgttctt cgttcttata aaaaaggaca gatccttttc gacgatggtg atcccagaca
     8461 acggttgtat atcttgacca aaggactcgt tcgtttagaa cgttatgatg aaagtgcaac
     8521 ttattactac tatgactatg tgaatacaaa taaccttttt cctatgtcag gaatgtttgg
     8581 agaagagacc tacacttata cagctcaagc gatgactgat attgaaacat tctaccttcc
     8641 tgtttcctac tatgaaaaat tagcacgaaa aaatcccgaa cagttgatct atatgataca
     8701 aaaaatttct aaagtagttg aaatacatga attgcgtatc caaatcggat tgacctctag
     8761 cgcctttgac cgggtaaaac aaaatttatt cattttgaaa gaagaattgg gcgtaccaac
     8821 aaaagatggt catatcacca ttccttatcc gatcacctta aaagaattag cagtgaatag
     8881 cggcacgaca cgtgaaactg ctggacaagt aatcaaacaa ctacgtgatt ccgggctgtt
     8941 agattatgac aaaaagcgtt ttactttctg taagacagct actactccag ctatgagaga
     9001 ataacgcgaa caaaaataag cagtgaccct gataaatatc agggtcactg cttattttcc
     9061 tccctaaaac ttctgaaaga tccggcagtt ttagatcact ggacaagact caaagatttt
     9121 ctatgtgtgt ttcagttatg taatattcac tgtttgcaaa aaatactgtt agattaacta
     9181 gatgaagcgt cttgtcctca tccgatctag attattcgat attcctcgac aataaacgat
     9241 tactcagtga gggcaatcag aggaacggat aatgataaat aagctgtaaa aaatagcaaa
     9301 ataacaataa tgattaaaag tatgtaaaaa atagggcttt gacaaaaacg tgtcacagcc
     9361 ctatttcgat taaacggtgt gtacctgtca actcttcggt cacaagtcat aatgttcata
     9421 aacatgagga gctgtttttt gaaattgttc cttgtgaatc agagttaaac gacaggctta
     9481 cgaactcttg attaaacggc gttcaaaagc ttgcatacgc ggtaataaga acagccaggt
     9541 caaaaacatg agaagccgtt tttcctgggc tgttcttatt acttgatgca gggcagcttt
     9601 ttcacaacct ctttattcag ataaaaccaa gcttggatca gcattcaact gatagctagc
     9661 aaaatttctt ttttgcattt ccacatgtgc tgctgctcca atcattgcag cattatctcc
     9721 acaaagtcga agagggggaa tcactagttc tacctcaggg agtttagctg ccaaagcatc
     9781 ctgcaatcct tcacgcaacc cttgattggc cgctacacct ccagctataa tcagttgctt
     9841 gactggataa ttttgacaag ctcgaagtgt tttagtaatc aatacatcga tcacacttgc
     9901 ttgaaagcta gctgctaaat catttttgtc tagatcttct ccacgctgtt gtgcattatg
     9961 caccaaattg ataaatgcac tttttaatcc gctaaaactg aaatcataat tatcttcatg
    10021 gatcatcgct cgcggaaaat ggtaattatc ttttccctga tgagctagct gatcgatttc
    10081 ttttccgctt gggtaagaaa gacctaatac tcttccaact ttgtcataag cttctcctgc
    10141 tgcgtcatct cttgtctccc caatgatttc atatgatcca tcttcttgca tatagactaa
    10201 ttctgtatgt cctccgctaa caagaagtgc catcaatgga aactggaatg gtttaaccag
    10261 acgggctgca tagatatgcc ctgccatatg gtttacagga ataagcggca attggtgtgc
    10321 ccaagcaaaa gccttagctg cagaaatacc aatcagcaac gagccgacaa gtcctggtcc
    10381 ataagtgaca gcgactgccg ataaatcttc tgccgacaca ccagcttcta ccagtgcatc
    10441 ttccaagcat aaggtgattt gttctacgtg atggcggcta gctacttcag gtactacgcc
    10501 accaaaacgt ttatgactgt ttatttgaga agcaacgatg ttagataaga tctctgttcc
    10561 atttctaacg accgctacac tcgtttcatc acaacttgat tcaatcgcca gaatcaattc
    10621 ttcattcatt tcatcgtctc cgtttttaac tttctgctca taatgatcgc atcttccgtc
    10681 ggattgcgat aatatgattt ccgtttacct aatatacgaa acttttctga ttcataaagt
    10741 tttcttgctg cttcattaga tagacgtact tctaaaaaaa catgcagcga atcttttatt
    10801 tgttccgtaa tcagtaattg taacaaatgc cgtgcatgcc cttttccttg ttctgaaact
    10861 gcaacagcaa tatttgtgat ctcgacttca tctattactt tcgagtatcc aataaagcca
    10921 cagatctggt tatcttcagc taaaagcaga taatcagagt gaggctgttg gatgtctgcc
    10981 aaaaactgca ttttcgtcca aggtgaacca tgtggataag cattttcgct aatcatccat
    11041 acgcattctg ccaagtacgc ttcatcaaaa tctttttttc tgcaaatcaa ctaatctcac
    11101 tcattttcta aaagttttat catatccaac gcatcttctt ttgtttcagt gtaatatctt
    11161 tttttgatcg ttgttgcttg aaaccccaat ttccgataaa gtctttgtgc atcttgatta
    11221 ctcatacgca cttctaatga catcgtctca cagcgattca ttatagcaaa gttctcgatt
    11281 tcgtcaataa gcagcgaacc gattcgttta cgctgatatg ccggtcgaac ggcaatattc
    11341 gtgatatggc aatctaaacc gaacgctctt gaaccgataa aaccaatcat tcttccatta
    11401 tcaaagatac ctagatatag atgaggaagc ggtgacttca attctgatat aaaggcactt
    11461 tttgtccatg gcaattcgcc attatagact tctcgttcga catccagcag ctctttgata
    11521 tcttctgtag tcaattctct tagtacgtaa cgacgatcga tttgttcgac tgttttttct
    11581 gtataaatca ttttatcttg aaaaaagctt ctggcatacg ctctaaattt tttcaacata
    11641 actttcgtct ccaggtgtgt gcgtctccaa ccatttttct tctgcttcta ctcgttttaa
    11701 atagcgagga agaaaagctt gtttctctgc aactggttca actaaagcac ctaattgagc
    11761 cacagttaca ccacttggaa tatcccatgc tggaactgta ttaatcatcg cactaggcaa
    11821 tatttccttt atttcagaga agaatttgtg acaatcactc ccgacgaaat aaatagcttc
    11881 attcagcttt tgtagttgtg tgaacaaatc agaagcagaa atatgtacat cttcaagtac
    11941 agtttctaat ctattttctt tccagcggta tgccccagcg tatacatttt ttcttcgagc
    12001 atcaaacatg ggcacaataa tattatccac accgatgcaa ttagccgcga tggttttcag
    12061 actagatact cctactagct ctttatctaa tgtataagca atcgtttttg ctgtcgttac
    12121 acctatacga agccctgtat aagaaccggg accatcagaa accgcgattc ggtcaatgtc
    12181 ttttggactt atttgaagat ctgcaaacaa ttggtcgatt gcaggcatca gtgttacact
    12241 gtggttgcgt ttgatatttg tttgtatttg tccaagaatt tgttcgtctt ctaccacacc
    12301 gatcgccaaa gtttggtttg cagtatctat tcctaatgta atcatatatt tgatcctttc
    12361 tgctatgaca ttctcgcctt ccattgtagc atactttttt attctgagga tgaaaatacc
    12421 aaaggcaaaa ctagtacaat aatacatgat tatctgctgg cgggcaaatt ttgccccttc
    12481 ctgaaacaca aaaaagctgc ggtatcactc cattttccca cagcttttcc atcttttatt
    12541 tcgttgattc tcgaataatc aactctgttt ccacaataat ctttcttgaa accggtgctt
    12601 tggaattact taattgaaga atcgtctcca ctgctagctc tcccatccat tcggtataaa
    12661 cttttacagt agaaagcgag ggcgttacat atttcgctac actgacatca ttgaagccca
    12721 tcacagaaat atcttctggt accactaaac ctttttcttg aatcgctttc agtgcgccaa
    12781 tcgctaatgc atcattagct gcaaaaagcg catcaggaac atgcggattc gtttttaaat
    12841 attgcttcac tgcttgatat cctgactcga cagaaaaatc tgcttcgata attggacctt
    12901 cgtcgtagcc tttttctgcc aaaacttgtt gaaatgccat aattctggga tcttttagcc
    12961 gctgatggtt tttcttcgta tattctgttc cagcaagcat agcgatatgt tgcgctttat
    13021 gggagacaaa gtgttcaatg actttttgaa cacttgactt aaagtcgaca actagagagt
    13081 cgattccttg agaagtgccg tcagaatcta ccaaaagcaa ggcattttgg gatcctttca
    13141 tttctaataa ctgagcttcg tcgaattttc ctagtgcgat cgttccatct gccggaagat
    13201 cactaatgga atcccacgtt tctttcaata aagtaacgtt tgcttcttct gccttttttt
    13261 cgattcctag gcgaatagat aaataataaa gatcttcaag ctcttccgta tcgtcgtacc
    13321 actggatcag gcgaagcgtt tgcttagtat ttgctgcatt tttacgatgt ttcgtataat
    13381 ttaagcgttc tgctgcttca aaaatttttt gctttgtggc taagccaacg gaaagatcag
    13441 gatcatagtt gagaactctt gaaacagtag caggagaaac tcctgccaac tgcgcaatgt
    13501 ctttaattgt tgccatttgt ctctcctcct tttttacgtt tataaaatcg cagtaaatcg
    13561 ttcaaacgct gcctgtcctt tttcatctcg cttgaatact ccagcgtctt ccagtacacg
    13621 tgcaaaaacc tgacctacag atttttgtac gacatcggtg accgtatctt tcgtcaaatc
    13681 aggatgttct ttttttaatt gttctgccca cggacgatga tattcagcca tctggttttc
    13741 ttctcctaat aagaaatgct ctacttctac tagttctggt ttcaaacgag gtggtaaaac
    13801 agcaagcccc attacttcga tcaacccaat gttctccttt ttgatatgtt ggacatcttt
    13861 atgtggatga aaaatcccat caggatgttc ttctgaaaca ttgttgtctc gaaggacaag
    13921 gtccaactca aagtaattgt cccttcttcg tgcaattggc gtaatcgtat ggtgtggtgt
    13981 accatctgct gaaaaagctc tgatagatac tgtttcatcc gaatatgctt tccatttttc
    14041 caatactgag ttagctgctt caataagcaa gtttttctct gttgattgca aacggatcac
    14101 agacatcggc catttcacga ctcctgcgat gacttgcgga taatcttcta gtttgataag
    14161 tttttctatc ggtgcttttg ccataggaaa ttcatgacgt cctgcctgat aatgatcatg
    14221 agaaagaatc gatcctccaa caattggtaa gtcagcattc gaaccaacaa agtaatgagg
    14281 caagacttca gtaatccgaa gcagtctagc aaatgtttcc tttgtgattt tcatcgggcg
    14341 gtgttgttct gataaaatga tcgaatgttc attgtaataa gcataagggg aatattggaa
    14401 tccccagctt tcaccgtcta gattcattcg aataatccgg tgattggtgc gggctggata
    14461 gttcaagcga cctctatatc cttcgttttc catgcaaagc atgcaggtag gatagtctac
    14521 ttttaccgcc tcgcgttctg ctgcgatttg ttttggatct ttttctggct tagataaatt
    14581 gattgtgatt tccagttcac catactccga ctctgccgga aatttgatgt tttttgcgat
    14641 ggctcttgtt ttgatgtagt catttttctt acttaaatta aagaaatact cagttgcttc
    14701 ctctgggctt ttcgaatagt gctgtgcaaa gaatgcattg acgactgaag gtggcggtgt
    14761 gagaaaatcc attaattgcg cttcaaaaat ctcttgttct gctggcaatt ctgaaagaac
    14821 accattttct cttgccttag cgacaagctg atccaataag tctacagatt cttccttaac
    14881 tggtcttact tcaatgcttt ctagagcatc ttctcctacc atccctaaaa gacgattctg
    14941 taaatataag cggtccattt ccatccatcc gccagcttcg atcgcaagtg tggcaaaatc
    15001 acaaatcgtt tgactgattg acatatctca ttcccctatt cttcataacc atttggatgg
    15061 ctgacatgcc aatcccatgc tgttttgata atcgctttga tatctgtgta ttcaggtttc
    15121 cagcccagaa tctctctcgc tttttcactt gaagcgacta atcggctagg atctccagct
    15181 cttcttggtg ctatttctgc tggaatttct tttcctgtca cttctctagc tgcttctagc
    15241 atttccttga ctgagtatcc tttgctgctg cctaaattga agaagttgct ttcatttcct
    15301 tctttcaagt attccaaagc taaaatatgt gcagcaatca aatcttctac ttgaacataa
    15361 tctctgatac atgttccatc tggtgtatca taatcgtctc cataaacagc caaggctttt
    15421 ctttgaccaa gagctacttg taaaatgatt gggacaagat gcgtttctgg tgtatgatct
    15481 tctccgatag atgcatctgc ttttgctccg gctacattga agtagcgcaa agcgacatag
    15541 cgcatgccat atgcttggtc acaccacttc atcatttttt ccatcatcaa tttactttca
    15601 ccatacggat ttttaggatt agctggtgta tcttctgtga ttggagactc tttcggctct
    15661 ccataagttg ctgcagtaga agagaatacg attttattca cgttgaactc gtgcatgact
    15721 tcaagtaaga tttgcatacc gtatacgttg ttgttgaaat acatcaatgg tttttcgaca
    15781 gattctccta ctaaagagct tgcagcaaaa tggattactc cttcaatcgg ttccttttca
    15841 aatacggaac gtagaaattc tttatcccgg atgtctcctt cataaaaatg agcatctgga
    15901 tgtactgctt gtttgtgacc tgtgagcaaa ttatctacca cagctacttg ataccccttt
    15961 tgaactagct ggtctacagc gtgcgaaccg atataaccgg ctccgcctag tactaaaatt
    16021 gtcataaaaa acactccttt gattagtaaa attgtgtttg ttgattctat ataatttaaa
    16081 aaacgaagtt tacttttatg gtgaaaacaa acaaagtaaa atttcgtaaa ttcatatggt
    16141 aaatttataa taaaacattt agtaaacatt ttcaagggtt ttttgcaaag gtttacaaac
    16201 aaacgtaaaa ctagc
//
LOCUS       DANKAZ010000058        16087 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000058 DANKAZ010000000
VERSION     DANKAZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(647..1013)
                     /gene="ssrA"
                     /locus_tag="PWY67_001806"
     tmRNA           complement(647..1013)
                     /gene="ssrA"
                     /locus_tag="PWY67_001806"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1184..1957)
                     /locus_tag="PWY67_001807"
     CDS             complement(1184..1957)
                     /locus_tag="PWY67_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2058072.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2101..2451
                     /locus_tag="PWY67_001808"
     CDS             2101..2451
                     /locus_tag="PWY67_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2058073.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2432..3148
                     /locus_tag="PWY67_001809"
     CDS             2432..3148
                     /locus_tag="PWY67_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2058074.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3148..4596
                     /locus_tag="PWY67_001810"
     CDS             3148..4596
                     /locus_tag="PWY67_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2058075.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4666..5175)
                     /locus_tag="PWY67_001811"
     CDS             complement(4666..5175)
                     /locus_tag="PWY67_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058076.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5165..5890)
                     /locus_tag="PWY67_001812"
     CDS             complement(5165..5890)
                     /locus_tag="PWY67_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058077.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6113..8233)
                     /locus_tag="PWY67_001813"
     CDS             complement(6113..8233)
                     /locus_tag="PWY67_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2058078.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8463..9641
                     /locus_tag="PWY67_001814"
     CDS             8463..9641
                     /locus_tag="PWY67_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL2058079.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9657..10415
                     /gene="aroD"
                     /locus_tag="PWY67_001815"
     CDS             9657..10415
                     /gene="aroD"
                     /locus_tag="PWY67_001815"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL2058080.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10438..10923
                     /gene="ybaK"
                     /locus_tag="PWY67_001816"
     CDS             10438..10923
                     /gene="ybaK"
                     /locus_tag="PWY67_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL2058081.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10994..12247)
                     /locus_tag="PWY67_001817"
     CDS             complement(10994..12247)
                     /locus_tag="PWY67_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2058082.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12365..13714
                     /gene="gdhA"
                     /locus_tag="PWY67_001818"
     CDS             12365..13714
                     /gene="gdhA"
                     /locus_tag="PWY67_001818"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL2058083.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13826..15172
                     /locus_tag="PWY67_001819"
     CDS             13826..15172
                     /locus_tag="PWY67_001819"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL2058084.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15480..15866)
                     /locus_tag="PWY67_001820"
     CDS             complement(15480..15866)
                     /locus_tag="PWY67_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2058085.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(15915..>16087)
                     /locus_tag="PWY67_001821"
     CDS             complement(15915..>16087)
                     /locus_tag="PWY67_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2058086.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         5255 a         2861 c         3216 g         4755 t
ORIGIN      
        1 aaacgaagta cgtcttctga aaaaaagtgg gtatattcac agactatttt ttacggaggg
       61 gttatttcaa tcagtttact ttcttcaaca ctttattctt ttaatgttat agacgtttcc
      121 atgtcaaatt ttatatctat tattggaatc cttatttctg caattattac acaactactt
      181 cttgtgtttg aagagaagtc aaaaaataat taaagcaatt atgcctttta gtacaggaat
      241 tattacttct tacaaatgta tagcgaatgt gaagcctgct gttttaattg ttctagaaaa
      301 acttttgagt gaacaattat tttatgttag gatgaattaa tttctctaga gtttataatt
      361 aaaaaactta tttgaaggaa catgcttaaa ccataattgt gtttttcctt ccatcagcat
      421 aaaagttcac ctcaaaaaga gtgtacgaac aaaagttctt tttgtaaacc ttaaaataga
      481 ttttcctgaa tacgtattgg ggatttctaa atgtttgaat gtttttttga atgcaaaatc
      541 aatgcaaaaa attttgtatt tctctacttt tctctccttg accaaaataa aaaaacgttg
      601 atttaacaac gtttttcata catgtttatc tgtctcaacc atacatatat ggagacggcg
      661 ggagtcgaac ccgcgtccaa acacatcgcc acttaaaaat ctacgttcat agtcaactca
      721 tttaaggatt cactttacag cttgccgagt gacaggcatt ctgtatcgct agtctgatgt
      781 tctctttttc aacttacaga cggaaagttg aaacgtatcc cactaaagtt aggaccctta
      841 ctcgagcaca tgggcgatgc cgagaggatc tacgctaact gtttttaggc agctaaagcg
      901 taagagttgt tttcgttttt agcagttata tttaactgta acgtttttac gtagacgtaa
      961 cctacgaaac gcaattcaag ctcgactgtg cctgtcgaat ccgtaacgtc cccgttagct
     1021 aacaagggtt acgtgtattt ttcatacaaa attagtatag catatattgg gataaattca
     1081 ataaaaaagc gaatcatatc ccattattta tataataact gcccctttct taagaaaaaa
     1141 taagcgattt tcttaagaaa agaggtagct tcaaaaaaag atcttaaatc agatcaaaat
     1201 acttcaatgc gtggacaatt ccgccttctg tatttttctt tgtgataaag gtagaaagtt
     1261 cttttaatcc gtcataggcg ttgcccatag cgattttgtt gtcgcaagct tccaacaagg
     1321 caaaatcatt cggaccatcg ccaaatccaa aagtaggaac tttttccagc tgaagttctt
     1381 tttgcagacg tttgacacct gccccttttg agattccttt cttcactgta tcgatcgagt
     1441 aaggaccatt ccgataaaaa gttagctcag gaaaatgttc gtgatagtat tcgtcacctg
     1501 attgagataa gatcagcaac atatttactg tgttttcttt aaagcctaaa ggatcaattt
     1561 ccggaacatc tgaatgaatg aagtcgtaag cattgatcat tgtttggcta tgaccggtac
     1621 accagatttt ctgatcgtta tagtaagaaa gttcatgacc gttcaactgt acatgatcat
     1681 acattttttg acattcttct gaagaaaatg tttgagaata gatttcttcg ccgtctactc
     1741 gaatgaaaga accattcatg gtgaccgcag aagtaatgcc cgcatcttcc atgatagagt
     1801 ggatttctgc ttctgtacgt cctgtagcga tgacggggag aacattgttt tcttttaatg
     1861 cgttcatagc ttttgccact tcgggtgtga tttttgattt cgcatctagt aatgttccat
     1921 caagatcaaa gaaagtgatt gcacgataat ttgacacaaa taaaactcct tttttattgg
     1981 aataagttta tcttaacaaa gaatgaacaa aagacaaacc tttcgtgaag aattcctgaa
     2041 catattggtt cagtcaacaa tttagagtat actataagaa gagaatttag gagggaaaga
     2101 atgatttttt tcagtattgg tatcttattc ttaataattg gcagtctttt tcggattctg
     2161 ccctcaaaag gaaatctgcc tttttatggg tatcattcgc ctcttgccgc aaaaacagac
     2221 gctcattggc ggctggctca aaagacaagc ggaaactggt tttttcttat gggcttactg
     2281 atggcattga tcggctatta tttaaagaca tctgggcata cgaattattt tctgattgaa
     2341 atgcttctgc tagtatttcc aattatgcct atttttatta tgacagaaaa gaaattacag
     2401 aaatatgacc tggaaactgg agggaacgat aatgaatatt ttaatgattg aagataatca
     2461 atctgtttct gaaatgatgc agatgttttt cttaaatgaa ggttgggaag caactttcaa
     2521 gtatgatgga aaagaagggc tcgatgcctt tttagaaaac ccacaacatt gggatatgat
     2581 tacactagac ctgaatttgc ccacactgga cggtatgcta gtaggaagag agatccgcaa
     2641 agtttcaagt actgtgccga ttatcatgct gacagctaga gattctgaaa gcgatcaggt
     2701 gatcggctta gagatggggg cagatgatta tgtcacaaaa cctttcagtc ctctgacgct
     2761 tattgctcga atgaaagctt tgcatcgccg tagtgagttg gcagaagcaa gagaagaagc
     2821 agcagtttca gatgatcatt ttgatatcga gactagtcat ttcaagatga atacgaaaac
     2881 acgagaagcg tatctggaca accggccaat tgaaggactg acgcctaaag agttcgatct
     2941 attgtttact ttagcaaaaa aaccacgtca agtattctct cgggagcagt tgcttgaatt
     3001 agtctgggat tatcagtact ttggtgatga acggacagtg gacgcccata tcaagaaatt
     3061 acgacaaaaa atcgaaaagg tcggaccaca agtcatccaa actgtttggg gagtcggcta
     3121 caaattcgat gattctggtg ttgcctaatg cgttatttgt atcagcaact cttagctttt
     3181 tgcggaatgg tcgcgttgat cttattgatc gttggagttt cttttacaca actgacaaaa
     3241 caaacgattg aagaaaacaa ttaccagcaa ctgtttggat atgctgaatc agttgaaaag
     3301 acaacgcaaa cctatgccga tacgctcccg cagtttagtc aagatcaggc gtttcaaaat
     3361 gcattgctgc tgacagagca agtactgaca gaacaaaatg tgaactttat atttatcgat
     3421 aaaaacgaac gtgtcattta tccgactaat acagctttac tgaattttac cttaacagct
     3481 gatcaatgga aaaatctgag gaatggacgt caagaaaaat tcacatcgaa taaaaatatt
     3541 ttaggacaag cacaggcgac ctcttacgca cttgtgccat ttaatttgaa tcatgaattc
     3601 tatggagcac ttgtagttag tcaacctgcg cgaaatatcg ataacagcgt tcgtccaatt
     3661 acactgaacc tgttcaaagg atttattttt tccagtatcg ttgcagttat tgccagctat
     3721 gcttttgcag cattccaagt taagcgtatc aatcggttaa ggaatgcgac gaaagaagtg
     3781 acgaatggta attttgatgt acagctcccc gtacatgaca aagatgaatt tgatgagtta
     3841 gcagatgatt tcaataaaat gacgaattca ttgaaagaat ctcaagcaga aattgaagaa
     3901 caagaaaatc gtcgaagaca atttatggca gatgcttctc acgagatgag aacacctctt
     3961 acgacaatca acggacttct ggaagggtta gaatacaatg caatccctga aaatcagcga
     4021 gaaaacgcga ttaaattgat gaaaaatgaa acagagcgtc tgatccgttt ggtgaatgaa
     4081 aacttggatt atgaaaaaat ccgtacgaat caaatcagta tggtcattaa aaaattcaat
     4141 ggaacagaaa cattgaaaaa tattgttgca cagttagaag ctaaagcaga agcagcagga
     4201 gatacattga cattgaaagc cgttgatgat attgatgttt acgctgatta tgaccgattt
     4261 gtccaaatca tggtcaatat tattcaaaac gcgatacagt ttactgaaaa cggccaaatc
     4321 acgattactt tagaaaaagg ctatcttgaa acgatcatca cgattgaaga tacgggaatt
     4381 ggaatgtctg aacaacaaat gaaaagtatc tgggatcgtt attataaagt agatccttca
     4441 agaaaaaata caaaatatgg tgaatcagga ttaggtttac caatcgtgca gcaacttgta
     4501 cgcttgcata aaggaaaatt agaagtagaa agcgaactag gaaaaggaac aaaattcgct
     4561 gttcgtttgc ctgacgtaga aatcaaaaaa gagtaaaaaa caagcggtat aaaagatcat
     4621 gaatcgatct tctataccgc ttgtttttac tataaaatga ttgatttagc ccgcgggctc
     4681 taaacgatca atataaggac tttgagcaat agtagcatca tgagtaaaga gtaagatacc
     4741 ttgattttct tcatgagcta aataagaaag caagtggagt agctcttgga tctccaatac
     4801 actaagttca tcaaatgtat cttcaataac aatattgttt cgcttggaaa gcaaggcatg
     4861 aagtaattgc agctttactt tttcaaaaga cgtcaactca gctgccgcac aattaagcaa
     4921 ggaaggagag atatcaagtt tttcttgata ggccaaacga tcttgtttga tttctttttc
     4981 cggtacatcc aacaggacat ggtctttaag attcagataa ggaaacatcg gccaattttt
     5041 ttggatgatc gcaaaattat ccagttttgc aacaagaacc tcttttttca agtgctccaa
     5101 tattttatgc tgtttttcga gaggacacaa aataacggtc aagtgttcag catgaatcaa
     5161 atattcaggt ttcattacgt ccaggcctcc tactttgcaa atgatggata aaaaaagtga
     5221 aagggaatag actaagaatg agacagcatc cactgagccc aataagagtt tgggcaaatc
     5281 ccttaagtat atctgtgaaa aaaagatcat tttggaaatc gatggtaaaa aaagtctgtt
     5341 gatccatcgg aattgtaagt ccgcttaccg aagtcaagat atcacggata tctggcagtt
     5401 cagtagtctt taagacaaga aaattgatcc tttgaagaag cgcttcataa actgactgga
     5461 agcttacgat ccacaataaa accaagatac aacaaagaaa gagaggaaag aacagctgcc
     5521 agaaaataaa aaggaataat ttggttttag aaagaccagt caggcgccaa ttgatcaatt
     5581 cctgcttgtt ttgcctgact gaatagtaaa agaaaaagat aaaaaggaag gcaaatagaa
     5641 aaaataaact gaatatgcct tttatataaa atcggctagc taattctaaa gccggaagaa
     5701 gctctgtaga tgcagaagcg ccaataaatg aaagccgatc ttggacttga ttggctaaag
     5761 ccgattgcaa atggtaaaga gtcaggcatg ctatcaataa aaaagcagaa aagcttgaaa
     5821 ataaacaata gagtattgtc tgctttttgt aataaattaa tcgagcagta gcatagcgga
     5881 ttattttcat acttttcacc tctataaaaa gtatagaaaa tgaatatgaa taaagtatga
     5941 aattccttta gtgaaatcac gaattaaaac cagaaaatat tttgtaaaaa gcaagaggtt
     6001 gtgaagaagc tgccctattc tattaccgta gatgcaagct tttgaacact gtttattcga
     6061 aaagaggagc agcgacaata attttgtcgc tgctcctttt aaatttattt tgtcattcgg
     6121 aagaacttgt ggtcgttccg tgaagttctt ggtacgtttg cgttttataa agatcaacag
     6181 ttgattgtcc gttgtgttca ctaaagacac tagttgactt gtcaccaaga gatttttcag
     6241 cttcttttaa tttctctaag ccattcttgt agtcataatc actggtatca attgggtcta
     6301 atccactatt cgtatagaac cgaagaagat caccattatt gatttggtca gaaacagcta
     6361 actgtttatc aactttttct ttcagtgaat caatttcatt ctgtacttcc tcagtaggtt
     6421 cagtaatagc ttcaccagtt tcgttactat agatcgtacc accgtagtaa gtatattttg
     6481 gtgtaacaaa atcaccatca cggaaagaaa caagctgttc atgatctttc gagaatagat
     6541 cttgtcctaa ctgaatataa ttttttgtat ctacacctag taagtgaagt aatgtaggca
     6601 aggcatcaac ttgtccgccg taagtgtgat tgattccgcc ctgtgtttgt ccagggatat
     6661 ggatcatata agggacacgc tgcatttgtg cattgtcgta gtttgtccaa gtagaacttg
     6721 tttttccaac taattcagct agatttttat ttcttgaatt cgagacaccg taatggtcac
     6781 cgtaaaggac gatgactgaa ttatcataca agccgctttc tttcaaataa ttgaagaatt
     6841 cttgtacggc ggtatctaaa taattggctg tcgcaaagta accgttgatc gtttcatcat
     6901 ttgtattggc taacgggaat cctgcttcgt cattggtgaa ttttgaataa ggataatggt
     6961 tagatacagc aatgaatttt gaataaaatg gttgttgcag acgttccaag tattgtacgg
     7021 attgttggaa aaatggttta tcatgcaacc cgtattggaa cgaattgtca tcattaacat
     7081 cataataaga agaatcaaag aaataatcat atcctaaatt cttgtatgtc tcgtttcgat
     7141 tccagaatgt accagcgttc ccatggaata ccgcactagt gtaaccttgt gtttgactta
     7201 aaatatcagg tgcagcttgg aaagtatttt ttccgcctaa ttgtgtgaac aaggagcctt
     7261 gattcaagcc aaacaatgaa ttttccatca gcgtttccgc atcgcttgtt tttcctgctt
     7321 ttacttgatg gaaaaagttg tcaaaactga acgtgctgtt gctgtggtaa agactattga
     7381 tgaaaggtgt aacttcatgc tggagaccat tctcatctgt caatttataa tcgatcagga
     7441 attgctggaa gctttctaaa tggatataaa tcacattttt tcctttagcg agcccgaaca
     7501 aatcgtcatt tggtgctgca taatgttctt ttacataatt ttctacttct ttcatatcat
     7561 tgggacttgc ttctgcccgc acttggtttg tctggtaagt ttgaacaccg tcataaacca
     7621 tgaaagcatt caagccaaga tatttgacca aataatcacg agagaatgtt cgagtcagca
     7681 attctggacg atcagcttcc gccataaaaa gatttcctga gaaaatcata actgctaatg
     7741 tagagatcgc aaaagccatt cttgctcgga tcgggcgagg gtccaacttg attttttttg
     7801 caatcagcag aacagtcaag ataataaaat cgatccaata gatcacatca tatgggcgaa
     7861 acagtcgaat ggcactttct cccagaccac tcgctacttt cccagcacct aaaatcgtgt
     7921 tgatcgtaat gaagtccgtg aattctcgat aataagtgac attcgaaaaa acaagcaatg
     7981 tcaaaagaaa ataaatgacc atcatcgtga tataagcaac tctagggcgt ctgatataaa
     8041 gagcaattcc aagtaataac aatgtagttg caatagggtt gataaacaag ataaagtatt
     8101 gaatgccact ttcgattccg agatggaaat caactaaata ggcaaaaatc gttttcaacc
     8161 aaagtaaaac gactagtaaa gaaaaaaatc cgagcctctt attcaaaaag gcaggtgttt
     8221 ttatacattt caagataatc ttcctttctg cacttcttaa tcacagaagt gtcatgaaac
     8281 catgttacac agctcactta ttctcattta ttttactagt atgacaatat agtgtcaatt
     8341 gaaaccagaa gtttatgctg tttcattaag gttttcttta agcttcatga aaaaatgagc
     8401 agtaagacaa gtcctatgtt ttttgttata cttgggaaga gaaagctaaa gcaggtgaag
     8461 gaatgaaagt aaaagtaaaa aaaagagcaa gcaatcgatt gaaaaaagga tatccactga
     8521 tccaaaaaga agaccttgct catccccaaa aagaaaccac gcaatgggtg gaattagttg
     8581 attcgcaaaa ccaatttatc gcaaaaggat atctaggaaa gcagaataag ggaatcggtt
     8641 gggtattgag ccagcagaac atcccatttg atcaatcttt ttttgaagaa ttttttgtga
     8701 agtcaaaaga aaaaagaact ttcttttttt cagatgattc tactaccgct tttcgtatat
     8761 tcaatggaga aggagacgga atcggcggtc tgattgttga ttattatgca ggttatgctg
     8821 ttttttcttg gtataacgaa acgttatatg cacatcgtga attattgatt caagcttttc
     8881 gtgaaatcta cccggaaatc gtaggaggct atgagaaaat tcgatttgaa tcagtaaatc
     8941 tgcctgagag ccagttcatt tacggaaaag aggcaccgga acccttgatc gtaaaagaaa
     9001 acggagtaaa atttgccgtt tatttaaatg aaggattaat gactgggatc tttttagacc
     9061 aaaaagaagt acggggatca ttagcagaag gtttcgcttc tgggaaaaca gttttgaaca
     9121 tgtttagtta taccggcgcc ttttctgttg ctgccgcaat gggaggggca atcgaaacaa
     9181 caagtgtcga tctagcaaaa cgcagtttaa aaaaaacaca ggaacagttt gaagtgaatg
     9241 gtttggatgt cgatcagcaa aaaatcattg ttatggacgt attcgagtat ttcaaatacg
     9301 ccaaaagaaa acaattttta tatgacatga ttgtattgga tccaccaagt tttgccagaa
     9361 ataagaaaaa agtctttcgc gtagcaaaag attatgggga actggtcaag gatagtctgg
     9421 atattttagc agaagacggg atacttattg cgtctacaaa tgctgcgaat gtctcgatag
     9481 aacaattcca aacaatgatt gaagaggaat tccatgccaa aaatgtttct tttgaacaac
     9541 ttgctttata tcgtctgcca aaagatttcc agacgacgga taccttttta gaaggcaatt
     9601 atctgaaagt gtttgtttat caaattaaaa ataaccatta gaaggagcga tgcagaatgg
     9661 tttgggtaaa gggattgaat ataggaagcg gtagaccaaa gatttgtgcg cctcttatcg
     9721 gggccgtccg agaggaagta ttacgtgaag cagttcttgc acgtgaagca ggggccgatt
     9781 tagtcgaatg gcgagtcgat cattatcgtg aagttttttc ttttgatgaa gtagcagaaa
     9841 cattggttct tatccatgag atgcttgatg gactgccgtt attatttacg tttcgtacct
     9901 tagctgaagg cggagaacaa gacatatcga tccgtcagta tcgcgacctt tatgaacatg
     9961 tgatacacac aggattagta gatatggtcg atatcgagtt gttcaaagta gaaagtctag
    10021 gaaaaagact gatagaggaa atcaagtctt taaacattcc tttaattatc agtagtcatg
    10081 attttaaaga aacaccagca gaccctgtct tgctttaccg attgaatgtt atggaacatt
    10141 ttggagccga tatcggaaaa ctggcagtaa ctcccaataa tgaacgagat gttttgcggt
    10201 tgatggagtt gactagacgg gcaaatgctt ttgtctccat gcctattatt acgatgtcca
    10261 tgggaaatct tggtaaaatt tctcgtctgg ctggcggaac gacgggttcc gttatgacat
    10321 ttggttcctt aagcagcgat tctacttctg ctccaggaca attaccggtc aaagcattga
    10381 agcaaattat tcaattatta aatgaaccgt tgtagaacaa gtttgaggtg atattttttg
    10441 gcaaagaaaa aaatagtaaa aacgaatgca atccgtatgg tcgaacaaaa aaagattccc
    10501 tatacagagc acgaatatga gtgggacgaa agccacctaa gtgcgtcatc ggtcgctgaa
    10561 cagcttccag aaagccaatc tcgtatattt aaaacactgg ttgctgtggg caatgtgacg
    10621 gggcctcttg ttgctgtgat accgggagaa gccgaattga atctaaaaaa attagcaaaa
    10681 gtttcaggga ataaaaaagt agaaatgctc catttaaaag atttggaagc aacgaccgga
    10741 tacatacgtg gtggctgctc gcctataggt atgaaaaaac tatttcctac ttatttagat
    10801 caaattgcag aaagctatga acaaatcatt gtctcggctg gcagaagagg gctacagatg
    10861 gagttagcac ctcaagatat ctgtgcatta acgagcggac aatttgcaga tataaaacaa
    10921 taaatcatac gtataaaaga aagaaggagg ccgggatgtt gtttgtccag tctccttctt
    10981 tcttttttat acattatgct ctttgcccgg cattcattcc tgctacatgc cctgtagcga
    11041 aagccgctgt gatattaaag ccgccagtat aaccattgac atccagtaat tctcccgcaa
    11101 aatacagacc atcaatgatt ttactctcca atgttttagg ataaatttcc tttagagaaa
    11161 cccctccgcc agttacaaat gatttctcaa taggaaaagt tttgttgatt gatagcttaa
    11221 actctttgca tagttgaaca aaatcttgaa tttgtttttg agaggcttgt tgtcctgtca
    11281 gatgatccat ttcaagccgc tcaagaaaga aaactaacaa tcgctcaggc aatataccat
    11341 gccaagcatt gaccaagttc tttttggttg ctttactttt ttctgtcaat atatgaataa
    11401 gctcttgttt ggtttgtgta gggaaacaat ccagagaaag tgtcactggt tctcccgttt
    11461 tctctaattc tttattgaca aaacttgaac aacgcagtgc agccggacca gaaataccga
    11521 aatgggtgaa aagtaaatcc atcgtatgtt cagtcaatac acgacctttt ttatttaaaa
    11581 cgcgtaaagt gatgtcttgt aaagaaagcc cttgaagtgt tttctcctga atatacggtt
    11641 catccgatat caagggggac tctgtcgcat aaagaggcgt caccgtgtga cccactcgct
    11701 tcacgatctt gtacccatct ccagtagaac cagtagatgg ataagtccgt ccaccagttg
    11761 ttaagataat ggcaggagca tggaactctt cgaattccgt gcgtataccg taaattttat
    11821 gttctttgtg gatcaatttc tctactcgag tagagaatag aagcgtcaca tctagttctt
    11881 tcagacgatt gagcaatgct tcgataatcg tttttgattt atttgttaca ggaaacatcc
    11941 gtccatgatc ctcttctttt aaatgtacgc cttgtgattc aaagaagttc atgatatcga
    12001 aattattcca ttgcgcaaac gtactatata aaaactttcc atttccagga atatgtgcaa
    12061 tcaaatcatc aacaggacga ttgttcgtaa cattgcaccg accaccacct gtcatcagta
    12121 gtttttttcc tgcttttttg tttttttcga tcaagagtac gcgtgcgcct tgttcagctg
    12181 cactgatcgc tgccatcatc ccgcttgttc cagcgccgac tacgatcaca tcatacagtt
    12241 tttccattcc ctcacctcaa ggaaagtgta gcatatctga agaaagaaaa cagaaaaaaa
    12301 atgttttgag ccaaaagaga aaatgagcta aaatgaggga agataagcaa aggagcgtgg
    12361 aacgatgaca aacgcaacag aatatgttca agcaattcaa gaaaagctac atcaaaagga
    12421 tcaagggcaa gaagaatttt tacaagcaat cgatgaattc atgcccacgg ttatgtcttt
    12481 tttggacact catcccgaat atatcgaaaa gaatatctta gaacttttaa cggaaccaga
    12541 aagagttatt caatttcgag tgccttggca agatgatgaa ggaaactggc gagtaaatag
    12601 agggtaccgt atccaataca actcagctat cggcccttat aaaggtggat tgcgcttcca
    12661 tcctagtgtc aatctaagta tcttgaaatt tttggctttt gaacagattt tcaagaatag
    12721 tttaaccggt ctgccaatcg gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa
    12781 atctgataat gagatcatgc gtttttgtca aagtttcatg ctggaattgt caaaacatat
    12841 cggcccatcg attgatgttc ctgcgggaga tatcggtgta ggagctagag aaatcggcta
    12901 tttgttcggc gaatataaac gattgaaaaa atatgatact ggtgtgttga caggtaaacc
    12961 tttggatttt tggggaagta agatacgtac ggaagctact ggttatggat tagtgtatta
    13021 tgtcaaacat ctattgaacg aagaaaaaga ttcatttaat caaaaaacag tttttgtttc
    13081 cggaagcggg aatgtagcca tttatgcaat cgaaaaagtg caagagctag gcgggaaagt
    13141 tgttacatgt tcagattcta gcgggtacat ctatgatcca gaaggaatag atgtgaaact
    13201 attgaaagaa gtcaaagaaa tcaatagaca acgtttgacg gaatacgcaa accaacgtcc
    13261 ttctgctatt tatcatgctg gagaatctgt ttggacattg aaagaaaaag cggatatcgc
    13321 tttgccatgt gctactcaaa atgaaataga cgaaagcttg gccgaaatct tagtagaaaa
    13381 tgggatcaaa atcgtagcag aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt
    13441 tcttcatgaa gcaggcgtgt ggtattgtcc aggaaaagct gctaatgctg gaggagtagc
    13501 agtatctgct ttagaaatga gtcagaacgt ccagcgattg acttgggaaa gagagcaagt
    13561 ggatgagcag ctggatcaaa tcatggaaac gatttaccat acgtgtcgcc aaacagcagc
    13621 cgagtacggc gacgaaaaaa atctgcttct tggagcaaat gttgctggat ttgaaaaagt
    13681 tgccaaagca atggcagcag aaggtttagt ttgaaaaatg aaactttctt ggaaatattg
    13741 tcagcatttt gtttatttgc ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag
    13801 agtaaaccta taaggaggag aacaaatggc acacattcat tttgactatt caaaagtagc
    13861 accgtttgtc aacgaacaag agttagggta catgcaaagc caagtaacag cagcccacaa
    13921 cgaattacgg gaaggtactg gcgcaggaag cgacttccgc ggctggatcg atttaccaac
    13981 gaactatgac aaagaagaat ttgcacgtat caaagaagca gctaaaaaaa tccaatctga
    14041 ttctgaagta ttagttgtta tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat
    14101 cgacttcttg aatcacacat tcttcaattt attagatagc aacacaagaa aagcaccaca
    14161 aatcttcttt gccggaaatt caatcagctc aacatatatt gctgacttga tcgaagtaat
    14221 cggtgatcgc gacttttcag taaacgttat ttcaaaatct ggaacaacta ctgaaccagc
    14281 gattgcgttc cgtgtattca aagagcttct tgttaagaaa tatggacaag aagaagcaaa
    14341 caaacgtatc tacgctacaa cagataaagc aaaaggtgca gttaaagtag aagcagatgc
    14401 agaaggttgg gaaactttcg tgattcctga tgacatcggc ggacgtttct ctgtattaac
    14461 tgctgttggt ttgttaccaa tcgctgttag tggtgcagat atcgatgcat tgatgcaagg
    14521 tgccgctgat gcaagcaaag catactcaag cgacaaacta gaagaaaacg aagcttacca
    14581 atacgcagca atgcgcaaca ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa
    14641 ctatgaacca ggaatgcaat acttctcaga atggtggaaa caattgtatg gtgaatctga
    14701 aggaaaagac caaaaaggca tctatccttc aagcgcaaac ttctcaactg atttgcactc
    14761 tttaggacaa ttcatccaag aaggacgtcg caatattttt gaaacagttg tgaaggttga
    14821 aaaacctcgt aaatcaatca caattcctga acaagcagaa gatttagacg gtctaggcta
    14881 cttgcaagga aaagaagtgg acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc
    14941 tcatacagac ggtgacgtgc ctaacttgct agtcaaaatt ccagaaatgg atgcttatac
    15001 attgggttat acaatgtact tctttgaaat tgctgtaggt atttcaggtt acttgaatgg
    15061 aatcaaccca ttcgaccaac caggtgtaga agcttacaag aaaaacatgt tcgcacttct
    15121 aggcaaacca ggctttgaag atcttgctaa agaattgaac gaacgcctgt aagataaggg
    15181 tttttcgcta gatttaaata atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg
    15241 tacccattta ggtactcaaa catactgcat cgtttattca gaataagggg ctgtgacaag
    15301 acgtttgtca cagtcccttg tttgtataga aatcgattga ttgccttgct ctttagtttg
    15361 ttttaggact ttaagaacag tgaggctgac ttcgaaggtt aattctgttt ttttgttttc
    15421 tagaatcagt aagcaaaaaa gcactcactt cgagtgcttt tttgcgatat attcttagct
    15481 taagataggc ctaatttttc ttttacagat gagggtacct tatcgctggt aattactttt
    15541 ttctctatga tccgcttttc gcttgctttc aactgcaaga tcgttccttc ggggtaagca
    15601 gaaccattgg cagagtcacc agaagtaatg acatcgaaat ctaactgtct tttttcaccg
    15661 ttttcagcat aagcatctac gttgtattta aaacctcttc caattacttc cccattatct
    15721 gctttgatat cagtttctgc aggcattgga gctttgacta ccgcataata atctttacct
    15781 acataagtag actgatagta gtcccaacct ttccatccta taaaagcaat caatatgata
    15841 acgatcaacc ctaaaatttt tttcatgaaa gatctccttt tttattttta ttatacccgt
    15901 ggtgctagtt aattttaata tcctaaagaa tatctcaaag ttccttcaaa acgtcgttgt
    15961 gctcgactta atagaacact gtaagccagt aatatgcttt caaatctact ctctagccct
    16021 ttaacagaac gtgagttgaa attttgacaa cctaattttt ctaaggaaga aaaaacagtc
    16081 tcaactg
//
LOCUS       DANKAZ010000059        15950 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000059 DANKAZ010000000
VERSION     DANKAZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(387..809)
                     /locus_tag="PWY67_001822"
     CDS             complement(387..809)
                     /locus_tag="PWY67_001822"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL2058087.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(823..2229)
                     /gene="atpD"
                     /locus_tag="PWY67_001823"
     CDS             complement(823..2229)
                     /gene="atpD"
                     /locus_tag="PWY67_001823"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL2058088.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2252..3154)
                     /locus_tag="PWY67_001824"
     CDS             complement(2252..3154)
                     /locus_tag="PWY67_001824"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL2058089.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3168..4724)
                     /locus_tag="PWY67_001825"
     CDS             complement(3168..4724)
                     /locus_tag="PWY67_001825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL2058090.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4747..5289)
                     /locus_tag="PWY67_001826"
     CDS             complement(4747..5289)
                     /locus_tag="PWY67_001826"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL2058091.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="PWY67_001827"
     CDS             complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="PWY67_001827"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL2058092.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="PWY67_001828"
     CDS             complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="PWY67_001828"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL2058093.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="PWY67_001829"
     CDS             complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="PWY67_001829"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL2058094.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="PWY67_001830"
                     /pseudo
     CDS             complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="PWY67_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10026..10781)
                     /locus_tag="PWY67_001831"
     CDS             complement(10026..10781)
                     /locus_tag="PWY67_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2058095.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10884..11120)
                     /gene="secG"
                     /locus_tag="PWY67_001832"
     CDS             complement(10884..11120)
                     /gene="secG"
                     /locus_tag="PWY67_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL2058096.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11238..11516)
                     /locus_tag="PWY67_001833"
     CDS             complement(11238..11516)
                     /locus_tag="PWY67_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2058097.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11629..12597)
                     /locus_tag="PWY67_001834"
     CDS             complement(11629..12597)
                     /locus_tag="PWY67_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2058098.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12812..12856
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12880..13854
                     /locus_tag="PWY67_001835"
     CDS             12880..13854
                     /locus_tag="PWY67_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDL2058099.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13859..14434
                     /locus_tag="PWY67_001836"
     CDS             13859..14434
                     /locus_tag="PWY67_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2058100.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14501..15640)
                     /locus_tag="PWY67_001837"
     CDS             complement(14501..15640)
                     /locus_tag="PWY67_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL2058101.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4357 a         3443 c         2856 g         5294 t
ORIGIN      
        1 ctgagccaga atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt
       61 attgacaaag agcccaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat
      121 catctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg
      181 tttctctaat actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga
      241 agtagcttct tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa
      301 attccttctt atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa
      361 acacggataa acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt
      421 gctcggtgca acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct
      481 tcttcgattt ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca
      541 cgttcacgtt ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt
      601 ccgccattaa cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact
      661 tcatcaatcg tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca
      721 gctgtcgtac gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt
      781 accacattta ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt
      841 cgctttttct acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc
      901 atcgtatttg ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata
      961 tgaacctgga agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg
     1021 gactctgcgg gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa
     1081 gatagcaatg atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc
     1141 tactttataa tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa
     1201 tggatctacc gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc
     1261 atccaaatgg gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata
     1321 gattgcttgg atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg
     1381 acccatttct gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa
     1441 ggctgatact tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac
     1501 atcttgacct tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg
     1561 catacgtgca cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac
     1621 tcctgaatct ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc
     1681 ggtaaataca gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag
     1741 tacggtttta ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg
     1801 ggcaagcaaa tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc
     1861 gtcaaaggct ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt
     1921 ttctaaatcg atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac
     1981 aggaacagag attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt
     2041 tgattccatc gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac
     2101 aacttttgat ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa
     2161 ggattggtct aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc
     2221 tgaactcatc ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa
     2281 tttcggtaat ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct
     2341 catcaatgat attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat
     2401 gttctgctgt tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca
     2461 ataactgaga caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag
     2521 ttgcttcttc tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag
     2581 ttagcgaatt gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt
     2641 acatagttgt tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata
     2701 gattgcgcag ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc
     2761 cgccaattgc gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca
     2821 tcgtttgttt caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga
     2881 cgatataacc cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc
     2941 ctctaggatt ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt
     3001 ctcttacttt tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact
     3061 tagaagcaga caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga
     3121 tacgtgtctt gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga
     3181 atggatttca atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct
     3241 ttatattcct gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg
     3301 attgtttcaa agatatctga atgattggta tccagatatt cgaataattc atgttcgaag
     3361 tctaaaatac tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc
     3421 actacttgtt tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta
     3481 cggcgtccac gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta
     3541 aatgcttcta attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc
     3601 attgctttga tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct
     3661 ggacgagtac ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa
     3721 ataacgtttg ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag
     3781 gcagtcattg aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt
     3841 gagtgtaagt agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc
     3901 aaggaaagtt cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca
     3961 tgtttcccgt tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa
     4021 agcaacggcg ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca
     4081 tattttttca atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca
     4141 tagatacaga tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt
     4201 ttccctgttt tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga
     4261 gcgtcgattg cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg
     4321 cctggtgcca ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca
     4381 attggctgac ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc
     4441 atgatctttc ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg
     4501 ataatgatac ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa
     4561 aattcaagca actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca
     4621 cctacataag ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt
     4681 tgttctttaa tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc
     4741 tttgtactat ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc
     4801 gattactcga tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg
     4861 ttttaattcg actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg
     4921 ttgttcgtct gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc
     4981 atcatatcga cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc
     5041 ataaacaact tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat
     5101 gatttctcgt ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg
     5161 tacttgttca tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt
     5221 tgaatcgatc gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc
     5281 tagtttcatt tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt
     5341 ctggagataa ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat
     5401 cgtcttttac agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac
     5461 tttttagacg agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc
     5521 cactttcttt cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt
     5581 gttcgcgttc ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat
     5641 cattggcgat cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat
     5701 gcttcaagag agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca
     5761 tcgcattgcc aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt
     5821 gaagtacttc tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat
     5881 actgattcaa ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa
     5941 cagcttcgac tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag
     6001 gttggcgagc cattgattcg attgttttag agataacttg accgttacca taaccggctc
     6061 cgattgctgc tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc
     6121 taaattagat aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta
     6181 tggctcatat aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac
     6241 aaggagaatg cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct
     6301 aagcttatta ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca
     6361 taaagacgta aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac
     6421 ataaaactat atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc
     6481 accccaaata agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg
     6541 tctgctgtcg ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa
     6601 ccgatgttgt ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg
     6661 ctcaattctt ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat
     6721 tctatcgcat tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct
     6781 aagaagaaaa caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa
     6841 attggtccaa tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg
     6901 aaattcacat aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac
     6961 acggtatcat accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt
     7021 ttttatttca gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga
     7081 aaaaacctat gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta
     7141 aaagcaaaag caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa
     7201 tgttcgatca atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga
     7261 ttttttccct ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt
     7321 taaaggaaca acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt
     7381 tttgtgtaat aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc
     7441 ataaggactg atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc
     7501 atcttttaga ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat
     7561 ccctgcttcc atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat
     7621 taattttccc tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt
     7681 cttcttttta acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt
     7741 actatcgcta tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt
     7801 tccttcgcgt tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc
     7861 ttctgcggaa acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt
     7921 tactcgcact ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa
     7981 atgattttca acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc
     8041 tactgtattc ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc
     8101 gaattcttcc cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg
     8161 ttctgtttct actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc
     8221 cgtagcccac gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg
     8281 atggacgatt agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc
     8341 cgctaagcca taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag
     8401 caacatagta ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg
     8461 taaatctttt ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc
     8521 aaaatcaaag aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa
     8581 cggcagattt aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga
     8641 ttcgatcaag cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc
     8701 agcatcaaca aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg
     8761 catcgtttca aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc
     8821 atagcgttgc atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt
     8881 catccgttct gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt
     8941 tggatcaatc tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca
     9001 gataccattc gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact
     9061 tgtccctctt tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac
     9121 atctgcaatg tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc
     9181 atcatctaga tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc
     9241 tctacgtcca gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa
     9301 tacatcatca ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat
     9361 gccagggtca ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata
     9421 ccctttttca gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac
     9481 tccctctgct gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac
     9541 gccgtaaaga tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa
     9601 ctgagtagtc gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc
     9661 tgtatttgct cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt
     9721 cgcttctttt gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc
     9781 tcgttcattt gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt
     9841 tcctttttta ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag
     9901 taatttaaaa tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa
     9961 actgcgtttg cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt
    10021 catatttatt cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat
    10081 tcgcgtcttg ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc
    10141 actcgctgac ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca
    10201 tctttcccag cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg
    10261 attgctgcct gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact
    10321 gctttttcat tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat
    10381 ttttcaaagt tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg
    10441 ccgccaatca ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat
    10501 cctaatacgg caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg
    10561 tctgcccacc agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt
    10621 cctttaaaaa gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt
    10681 cgaacatcgt ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt
    10741 tttgcataaa ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac
    10801 catagtttct ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc
    10861 cgttttgcga gaggcttatt caactacttc gaagataaat atgctaggac aaataataag
    10921 atcatccaga tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga
    10981 gctttttgtt tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt
    11041 tgcttgcttg gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt
    11101 cccaaaatta aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt
    11161 tatcatagat aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa
    11221 actttcagct gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca
    11281 aactcttttt ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat
    11341 tctactccag tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca
    11401 aatgtatggt caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc
    11461 tcttgttgtt catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc
    11521 cgcactcctc aaataactga tattttttta ttcctacctt attttagcag tctcctgatc
    11581 tataaacaat tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt
    11641 tattctttgt tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga
    11701 tgcttccaag tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt
    11761 ttgaatcgtg tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa
    11821 taaaggcaga tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc
    11881 ggcgtatttc tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt
    11941 gattttactt aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat
    12001 aaatcctttg accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc
    12061 cattgatccg ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat
    12121 ccaggcaatt aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc
    12181 tccgtgcgca cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc
    12241 tatatgagac atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt
    12301 attctgctta tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt
    12361 gattgttttg ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg
    12421 ttttttttct actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata
    12481 ccagtcatat cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag
    12541 aaaatggtaa ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt
    12601 acacccgccg ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg
    12661 attctttatt ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag
    12721 ttctcctcaa tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac
    12781 taatttcgtg cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat
    12841 aaaaaactaa gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat
    12901 tgcgatccag ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc
    12961 gaagtagttg ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat
    13021 gtgctgaaat gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa
    13081 aaacctttga agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg
    13141 gaaacaaatt tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt
    13201 ttggctgatt atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc
    13261 aatatcgctt ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc
    13321 tcgatgggtg gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac
    13381 tggtgtgatc ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg
    13441 gttgggttag atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt
    13501 caaatgaatc ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc
    13561 cactggaaac aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat
    13621 tttatcgagg gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga
    13681 ataagtcgtg gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt
    13741 aaaattatga caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta
    13801 aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat
    13861 gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa
    13921 ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg
    13981 aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag
    14041 cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat
    14101 tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag
    14161 cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat
    14221 cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct
    14281 attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga
    14341 ctaccttgat cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca
    14401 acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata
    14461 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
    14521 taattccttg atatttttca atcatatcca aataattatc gatttcaaca tactcatcta
    14581 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
    14641 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
    14701 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
    14761 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
    14821 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
    14881 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
    14941 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
    15001 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
    15061 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
    15121 gcgtatacat caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta
    15181 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
    15241 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
    15301 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
    15361 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
    15421 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
    15481 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
    15541 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
    15601 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
    15661 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
    15721 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
    15781 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
    15841 gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa
    15901 acatcatata cagaagctag ttgcaaaaaa aactctctac ctgctgatat
//
LOCUS       DANKAZ010000060        15163 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000060 DANKAZ010000000
VERSION     DANKAZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..261
                     /locus_tag="PWY67_001838"
     CDS             <1..261
                     /locus_tag="PWY67_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2058102.1"
                     /translation="KRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSH
                     SNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(644..1372)
                     /locus_tag="PWY67_001839"
     CDS             complement(644..1372)
                     /locus_tag="PWY67_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDL2058103.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1350..2924)
                     /locus_tag="PWY67_001840"
     CDS             complement(1350..2924)
                     /locus_tag="PWY67_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2058104.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            3153..4091
                     /locus_tag="PWY67_001841"
     CDS             3153..4091
                     /locus_tag="PWY67_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2058105.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            4103..5014
                     /locus_tag="PWY67_001842"
     CDS             4103..5014
                     /locus_tag="PWY67_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2058106.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            5038..6510
                     /locus_tag="PWY67_001843"
     CDS             5038..6510
                     /locus_tag="PWY67_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2058107.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6561..7172
                     /locus_tag="PWY67_001844"
     CDS             6561..7172
                     /locus_tag="PWY67_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2058108.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            7202..9115
                     /locus_tag="PWY67_001845"
     CDS             7202..9115
                     /locus_tag="PWY67_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HDL2058109.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            9243..12224
                     /locus_tag="PWY67_001846"
     CDS             9243..12224
                     /locus_tag="PWY67_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HDL2058110.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12221..13882
                     /locus_tag="PWY67_001847"
     CDS             12221..13882
                     /locus_tag="PWY67_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HDL2058111.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13922..14854
                     /locus_tag="PWY67_001848"
     CDS             13922..14854
                     /locus_tag="PWY67_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDL2058112.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4927 a         2409 c         3043 g         4784 t
ORIGIN      
        1 aaacgaggta cggaaggctt tcaagaaacc cttaaaacaa tttcatctac gttacctcag
       61 tcgtttaaaa agaaattcac gatcttccat tgctaccaat cgggtatttc aaatgccttt
      121 aaggtgtccc actcaaacgg cgtcactgag gggctgaata acaagatcaa gctgattaaa
      181 cgaattgcgt ttggttatcg caacttttat aactttcggg cacggatcta tttacaacaa
      241 ggtctgatct ttgaaaacta aaaaaatatg cggcgcacag aacgcgccgc atatcggact
      301 aagttttatt caaatttgtc ttcaccaaca cgatttgacg aagagcctat ttaaataact
      361 acatccattt attctatctt ctaaaatagc ctttttcatt tcagttaaat agttcagcgc
      421 catttttgca ctcgacttgc atagtagttt tttccatatt cctcaacctc agcgggggtt
      481 gaagacataa ggaaaatcct atgcgttatg ctcaactgga tctgattaaa tattctaatg
      541 tatttttctc taccatcatt aaatggttaa tagccaaaaa tcaccacttt tcaatggctc
      601 cttgttgtat aagtataact agaagatgaa cacgtctgag aaattataga agctttttac
      661 ggtaattact ggggctgtgt ccagtagttt ttttgaagag aacagaaaaa tagctcacgt
      721 gatctattcc tactttttga ctaatttcat caatagataa gaatgtgtct ttcagtagag
      781 cacttgctct tttgattcga aagtctagca agtaatcatg aaaggaataa cctgtttctt
      841 ttttgaaggc ccgtcctaaa taagcgggat ggtaatggaa ttgtgtagct acatctttga
      901 gcgtaagctt ttcactataa ttgttttgaa cgtaatcaat cacttgttga attgactcat
      961 taaaagaatc atcagttaga gagttttgat tagctgctga tttacgcatt ttttcttctt
     1021 gtactaactt aactaaataa ggaaaatcaa tgggtttcgt tatgtaactt ttaatagata
     1081 aatcaatccc cttttttgca tactgaaaat tttggtgggc actaatgaca atactagtaa
     1141 agtagcgatt ggatgtttgt tgcaaagatt cgattaattc gagtccagaa attttcccca
     1201 tcgtaatatc tgtgataagt aaatcaaagc gctggttttg caggttttta aatgcttcgt
     1261 ctccgtctgt gtagcagtga tcgataatga tgtcggggac tttcttcaaa agataatgcc
     1321 ttatgccttc tacaattgta caatcatcgt caacaattgc aattctcaac atgcatattc
     1381 cccccttttt gtattttgat agtaatcgtt gttccgacgt gtagttggct ttctataaca
     1441 aaacgtgtat ctccaggata gagatgcttt atacgtcgaa aagaatttga gatgccgata
     1501 ttctcactat aaggaatatc atcattaagt ttttgttgaa tatgctccag cttgtcttgg
     1561 tccattcctt tcccattttc aacgattttt aacacataat ggttggttgt ttcagttgca
     1621 tagacagtca aacgattttc cttttttgat gaatcgaagc cgtgtttaat ataattttca
     1681 acaaatggct gtaaagaaag ttttggtaac attaattgat caatatgatc aatcagctga
     1741 tagtcaaagt tatgtcgata acgacgttga tgtagttgca aggagaggtc gatattaatt
     1801 aattcctctt tcaaatcaat tatgtcgggc tgatatgtat tattgcgata caacttgctc
     1861 aataaatata tactgtcgct gatttcttcg tcatcattca atatggcagt cattcgaata
     1921 aattccaaat tattaaatag aaaatgaggc tcgatttgat attgaagtgc tgcatacttt
     1981 gcttcttgct tcaaccaatt attttcataa agtgtttttt gatatgtagc attatctaaa
     2041 taaaactgat tgatatgcat agcgatttct ttcaaatcac cgactttgtt ttcgatattg
     2101 attgacttgt agtctcctgt agatgaatga tttaaggaag taatgatttc ctgatactgc
     2161 tggatatagt tacgaaaact tctatttaat aattgatgaa tagcaagaat aaacaaactc
     2221 attaataaga aaatcatgat tgccagacct aataaggttt gtgacgaata atttggattt
     2281 tcatatactt gtaatgtgtg gttaccgata gctatctgtt gcgttttttt ccatttagaa
     2341 ggcgagatgt cttcagataa attgacggaa tcaataccat tagacaaaga ataattgtaa
     2401 tgtgttggaa agagactctt ttttagcgcg ttgatcgaaa cgtctagaat caatgtagca
     2461 tcgggcatgt cctcactaat gaaacttaat gaacgaataa aagaaatttt ttcgtttttt
     2521 gtaggtgcgt aggcacttaa gtatttttcg taatctaatg agatagatat atttttaact
     2581 tcatcatctt gcacaagttt ttttaagtta tctactaaat aggtagggga gcgatccttt
     2641 gacaaaatat cttccatgta ttcagcaggt gatgagaggt agtagtcatt aaggttttct
     2701 tggaaatcat aattttgata gatctctgtc acgataccgg agagaatacg attatatgtt
     2761 tccatatagt gggtgatttg ctgaatgttt tgcaggtttt gatttttttc tttttcaatc
     2821 acattgatat aggaaaagaa caatgtgaaa caggcaagta aagtaataaa aaaaatcaat
     2881 atgtatgtaa aacgcataat aacttttgat aaatcgtttt tcattcaaag tcctcatttt
     2941 gataatctct aattttatga atcattatag cacatattac aagtgatgta aacgctttgc
     3001 aaacagggct acttatgatt gtgaatacag tttccgttta aaaactattc tattgtagag
     3061 gtttaataaa ctaaaaataa tgtataaaaa attaatgtaa gcgattaacc aagtgggtat
     3121 aatcaaggaa acgctgagag aggagagaca aaatgaagct aaagaaaggg ttattaggaa
     3181 atctaaaaaa aaaccgagca ttgtttatta tgcttttacc aggtgcgtta tggttattta
     3241 tattttgcta tataccaatg tttggagttg taatcgcttt taaagatttc agaatttcat
     3301 caaggggatt ctttacaagt ttgttcaaca gtccttgggt tggctgggaa aatttcaaat
     3361 atctctttgc ttcgcaagat gcttggatca ttactcgcaa tacggttgtt tataacgttg
     3421 taggtatttt gctaagtact ttttgtacag tgtcattagc aatcattctg agccaaatgg
     3481 cgaacaaacg gttattgaaa aagatgcaga catttatgat ttttcctaat tttttatcat
     3541 gggtagtcat cagttacttt gtttacgcat ttttgagtac ggataaagga ttaattaata
     3601 ttttactaag caattttggt gtggaaccca taaattggta tagtgaaccg aagtactggc
     3661 cttttattat tgtttttacg agtttatgga aaggcgtagg ctatggatcc atcatttatt
     3721 acgcgtcaat tatggggatc gatactagtt attatgaagc tgctttagtt gatggtgcca
     3781 gtaaatggca acagataagg aatgtagtct tgccacattt aagatcgatt attgtcatta
     3841 tgttgatttt gtctgttggt agtatttttc gagctgattt tggattgttt tatcaattgc
     3901 caagaaattc gggggctctt tatgatgtga cgaatgtatt agatacttat gtctaccgtg
     3961 gattaatgaa tagcggagat atcggaatgt ctacggcagc gggattatat cagtcatcag
     4021 ttggctttgt cttgattttg attactaata agatagtcaa caagttagat cctagttcag
     4081 cactatttta ggaggaatca aaatgatatc aaaattaaaa cggcagatgt acttttcgct
     4141 gccagtcgaa cggtttttca caatattact aatcttattt actcttagct gtatttttcc
     4201 ctttattttc gtaatcgtca tttcattaac agatgaaaaa gcattacaac tcaacggtta
     4261 cgaattattg ccggctcaat ggagcactga tgcatatcaa tacttaatac aagatggcgg
     4321 acagttactt cggtcattag gcgttacaat tatgattaca gtgattggaa cgttgattac
     4381 tgtttttatg acgggaacct atgcttatgt tttatcaaga gcctctttcc cttaccggaa
     4441 gttctttact ttttatcttt tttttaccat gttatttgcg ggagggatgg ttccttctta
     4501 cttagtgatg acgaagatgc ttggcttaaa aaatacaatt tgggctttga ttctacctct
     4561 tgcttttagc ccatacaatg tcattatttt aaggacattc tttaagaagt cgattccaga
     4621 atcgattatc gaatccgcta aaatggatgg ctgtagtgag tttcgagttt ttttccaaat
     4681 cgttttgcct ttagcgatcc caggggttgc aacaataggt ttattttcaa gcttaggtta
     4741 ttggaatgac tggttcaacg cattgctgta tattgacacc aacaaattga ttccattgca
     4801 atatttgtta atgaagattc aaaattcaat ggaattctta gcaaataata atgacattac
     4861 attagcgcag caacaagcaa ttcaaaatag ccttcctcaa gaatcaacac gaatggcaat
     4921 ggtcgtagta gctactttac cgattgcaat tgtttatccg ttcttccaaa aatactttgt
     4981 tcaaggattg acgatcggtg gagtgaaaga ataaaaaaga ataggagatg ttaaaagatg
     5041 aaaaagaaca tgcagaaata tttgattggt ggtgcttctg tactaaccct tggcctatta
     5101 ggtgcttgtg gtagcagtaa tggcgaagca aataatgaaa caggaaccac aaaagttact
     5161 tggtatcagg ttggagaaga acccaaagat tttgatgagg tcatgacaaa agtcaacgaa
     5221 aaattaaagg aagaaatcaa tgtagagttg gatatgcggc tgattccgga cggggattat
     5281 cagcaaaagc ttggtgtgat gatcaattca ggagaagaat atgatatttg tttcgtcaat
     5341 ggcacagatt atgtgaatta tgggaacaaa ggtgctttca ttagtctcaa cgatcatttg
     5401 gataaagagt tgaaagatta tgcagctgaa ttgaatgaag gatttattga aggtggggca
     5461 attaatggcg aaacttatgg attgccagtc aataacgata ttgccacaac ggaatattgg
     5521 acctttaata aagatttggt agatcaatac caattggatg tagagaatgt tgaaggtgta
     5581 gaaacattgg ctccagtatt agaaaagttt gcagaaaaaa atactgatac gaatattacg
     5641 ccaatcggta tgacccgtac cagtgtctgt attccttatg acaatattat cggtggtggt
     5701 gtaccgctag cggttgcatt tgaaggcgat acacaaacag ttgttaatac ttatgagact
     5761 ccagaaatga tgacttattt gaaggctatg catgagtttt ataaaaaagg atatattttg
     5821 aaagatgcag ccactgttac gacagatcca tttcgaattg atagtaaaaa ctggttttta
     5881 ggtcgtaact caagtggtta tcaaccatta gcacaaaaaa aattagaagc tctagcggga
     5941 catggattaa ttttaaaacc agctggaaaa gcttttaaaa cgactggatt tgcaagagtg
     6001 gcaatgcatg cggtatctgc ttcaagtaaa catccggaag cagccttgaa attgttgaat
     6061 tatgtgaata ccaatcaaga agtagcggat ttaatggttt atggaattga aggaaaacac
     6121 tatgatcgca atgaagatgg ttcgattact ttaacaaact ctgatgatta ttatcaaggc
     6181 gcttgggcat tggtaaatac ttatctagtt tcaaaaactt caccgattac agaagaaagt
     6241 aaaaaactag aagaagaaag taaaaagttt caaaatgaag caccgatgtc tcctatttta
     6301 ggtttctctt ttgacgcttc tgattttcga acagaaattg caagtgtaca aaatatcgtg
     6361 tcacaatatg aagtactttt aagctcggga acagttgacc ctgaacaata tgtaccagag
     6421 ttgttagaaa aactagaaaa agctggattg gataaaattc aggcatctgc tcaagaacaa
     6481 tacgatgaat ggcgaaaatc taacaaataa ttttttacta aatgaaaaga atagactaag
     6541 cagttagtgg ggggattcaa atgaacgaaa acaaatggac caaaggattt aattgggttt
     6601 ttcaattgtt gttactaaac tttacttatt ttatactaat ttgtttagga tttggaattt
     6661 taggtttttt ccctgctacg atagctttga ttgctggcgt tgtgaatcga aaaaaatatg
     6721 gcttgcaaga gtctgtgaag ttcatgagta aaatctataa gaaaaatttt gttagatata
     6781 atctatattg ctggtcagca actttgttgt tggccggcaa tattactacg atctggcttt
     6841 gttggcagca aagcgcacca ttgttttatt tattaagtac attattatcg gtaattacac
     6901 tgttcctagt ctttgcaatg ttgaattggg tcgtgatcaa aagtcttggg ttgtggatga
     6961 tgggagaccg actgattgac tatttgcttt tcacagcaac gatgttgcca acggtagtca
     7021 tgcaaggctt ggtgcttgga atttatttcc gaatattgat gattttgcct gtgatttttc
     7081 tgtttggcgg aacagctgta acggtaatat tgattagtaa attaactgaa agagcgttag
     7141 caaagttgcc ttgtcagata caatcgcact aattttttca caaaaaggag tatttctttc
     7201 tatgaagtta actgataaac aagtagctaa tatagccttt atccagcaac ttgagattcc
     7261 attgacacaa aaagatgaag aacaaataga cttgattttt actgacacaa aggctaatgt
     7321 gcgacaaatc aagattacta aaaatgagca gcaacgaatt tgcttgtctt attcacatga
     7381 agacttgctg tatcgaggat tgtttatctg ccttccacgt tttttagctc aaggaggaga
     7441 gatgccatac attgaagaaa cctcgatcat tgagaccagt tattcattag atgcttctcg
     7501 taatggcgtg atgaataaag agactctaaa aaaattcatt tcttacttag cgttgatggg
     7561 gtacaacaca ttgtatattt acacagaaga tacctatgaa gtagaagacc ttccatattt
     7621 tggttacctt cgcggagcgt atacaaaaga agaagtgaat gaaattgttg aatttgcttc
     7681 tggttttggc atagaagtag ttccatcgat ccagaccctt gctcacttga cccaattttt
     7741 gaagtggtat gctcaacaag aactgatgga tgatcaaaat actctactga tcggtgaaga
     7801 aaaggtttat gcggcaatcg aaaagatgat tattgcctgc aaagaaatgt atcagacaga
     7861 gcgtattcat ttgggaatgg atgaagccta tcaagcaggt ctgggacgat atttaagcct
     7921 gtatgggtat tgtgatcgga caactttaat gattcaacat atggaccgtg tgttatcaat
     7981 tgttgataaa tataagatga aaccaatgat ttggagtgat ttcatttata agctattaga
     8041 tgaaaccggt aaagataggt attacaatcc tgattttaaa ctcaaagcat tagcagctaa
     8101 aggattacca gcaggattaa cctatgttca ttgggactat ggttcagatg aagtttcctc
     8161 ctatgagaaa gtaattgata accatttgaa attttgtgac attgatcatt acgttttagc
     8221 tagtggtgcc catatctggg ggagactagc cccgaatcat gggaaaacag aagcaattgt
     8281 ttctgcagct gtaaaagctt ctaaaaataa aggtgtacgt tctgtgatgt taactacttg
     8341 gggagatgaa gggcaggaaa cagagcattg gcattcctta attggggcat taaaattagc
     8401 agattatgtc tattctgaag aagagtggaa tgattttgag gaaatctacg atcgtttatt
     8461 aggaacaggt agttatcaat taatgtccga attacgatat ttcgatgaag tggataccat
     8521 tacaaataac aacttacaaa tggcttccgt gagtaaactg ctactttggc aagatccgtt
     8581 agttggtata tatgactttc atgtagccgc tcataacaaa attgccacta gaacaattgg
     8641 tacttattat catgagttag ctcagaaatt agatatgaat aaaaattgga atccaaaaca
     8701 accaatttta acaactattc gtcaacgata tatactttta gctgaaattt tagcacaaaa
     8761 ggcagaacta ggcatctatt tacaacactg ctgtctggta gaagaccttg caggattaat
     8821 ggaattagcg aatcggtgtg aagcactagc caatcgagta aatgaattgg catctattca
     8881 tgaaataatt tggtatcaaa catacaaaac ctatggttgg gaagtattag aacaacgata
     8941 tgctgcatta gctcatcgtc tgaaaaccgt tgcgcgtcgt attaaaaatc atcttcaggg
     9001 gcaagatgcc ctagaggagc ttttggaaga acgattaccg ttttgtcaat atcaagtgcc
     9061 gcttgaaatt tctggcttta attatcggca aagctcggtg tcaggctaca attaacgaag
     9121 gaatcttccc gttgaacgga ccacttgctc atctaatggg aaagtggttc gttttctatt
     9181 cataagtagc ttaccactaa ctcttttatt aagaagagaa aaaaagtagg aagtgagaaa
     9241 atatgttatc ggactattat caaaatctgt cagttataag tgtcaataca gaaccattac
     9301 gtaattatta tattccttat gcaagtaaag aagaagcaaa acaagagtta gaaagggaaa
     9361 aaacgagcca gtttatcgat ctcaatggta catgggattt tcagtattat ccttctgtcc
     9421 aacagttacc ggaaaatttt tttcaagagg cctttactga aagctatgat agaatttctg
     9481 ttcctggagt ttggcaaaca gagggatttg acttacctca gtatatcaat gtatcttatc
     9541 caattccttt tgatccgcct tatgtaccta atgatacccc ttgtggtgta tatcggcgtc
     9601 agtttcaaat tgagcaaaca gatgcacgct tttacttaaa tctagaagga gcagatagta
     9661 attgttttgt ttggataaat agaaactttg taggctatga ccaagtgagt catagtacat
     9721 ctgaatttga tgtgaccaag tatttacagg ttggggttaa tgaaattcaa attctagtga
     9781 tgaagtggtg tgatggtacc tactttgaag atcaggataa gtggcgaatg agcggtctat
     9841 ttcgtccagt gtatcttctt caacgaccaa ctatgcatct gacaagttat caactacgga
     9901 cgttgactga tcctaaaaaa ggaagcggaa cattaatggt ttcttttcaa cgatttactc
     9961 ctattgaagt atcgttacct atttcttacg aattattcca tcctgatgga cgtctcatag
    10021 cgaaagggga gtgttactcg gaacaacaaa taaatttgga tgttgaagat tgcctttttt
    10081 ggacagcaga aacgcctaaa ttatatacat tacttttaac atgtaatggt gaatatttta
    10141 aagaaaaagt tggctttcga gcactgacga tcaagaatcg taagttattg ctgaacggaa
    10201 cacctattaa attatatgga gtcaactatc atgacgtgaa tcagcaaggc acgccagtga
    10261 tgacccgaga gcagttacgt gcagatttgt tattaatgaa aaaattaaat gtgaatactg
    10321 tacgaacggc acattaccct aaatcaccag atttttatga attggcggat gagattggtc
    10381 tgtatgtaat caatgaagca gatttggagt gtcatggtgt ggttaatcta tatgatactg
    10441 aagcgaatta tcaactaatt gctaatgatc cgacattcga aaagccgatt atggaccgag
    10501 tgatccgttc tattcttcct tatcaaaatc gaagctctat cattatgtgg tctatgggga
    10561 atgaatctgg ttatggagta aattttgaaa aagctcaagc tttagcacgg aaattggatg
    10621 attcacggtt gattcataat gaacgctcga ttgaaccagt tattgaccaa gtgaatgatt
    10681 ttagtaactt ggatgttatt agtcaaatgt atcccccact ggaagatgtt catcgtcatt
    10741 gtcaaagaag tgatactgaa aaaccttttt ttatgtgtga atatagccac gctatgggca
    10801 acggtcctgg agatcttaaa acgtacttcg atttgattca acagtatgat tcctttcttg
    10861 gcggttgtgt ctgggagtgg actgatcatg caatcaatag aggaacttta gaaacgccat
    10921 cttatttata cggtggtgat tttggtgaaa aatatcacga cggcaatttt tgcgtagatg
    10981 gtttattgac cccagatcgg caattaacag taggtgcatt ggagtaccgt aatattcatt
    11041 cgcctattca actgattgct gtagatgagg agctgcaaga agtaaccttg agcaatataa
    11101 ctgcttttac agccggttca gcatttaacc tttctcttga atggtatata gatggaaaaa
    11161 aaacagttta tgaagtaatg aatcctttca tacttgagcc tggcaaaagt tgttcattgc
    11221 ccatcacagt acctacaacg attcatggtg ctcatacggt tgctgtttcg ctatctgttt
    11281 ataaaaatga tgagctttat ggacagcaac agctctttct taaaaaagaa aaaatgagat
    11341 tgccaagaac tgctcatcat aatgatgcac cacaaatttc agtaaaaggc acagagctga
    11401 taatcaaagg agaatccttt atttatatat atcaattagc aaaagctgcg ttcacttctg
    11461 taaagatcaa tcaaaaggaa cttttggaac gtcctactca attttcaatt tggcgtgcac
    11521 caacggataa tgatcgtaac ttagtaaaca aatggaaagc agctggatat gctgaaatgt
    11581 ctttagaaat cagaaaaaaa gactggaaat tcgtagatga tcatctcctg atcgaaacgt
    11641 cttatcgagg agttattcca ggattacagc caatttttac aatgcacata cagttgaaaa
    11701 taaatggtgc aggcgtaatc aaccaaaaag tatcagtgga gaaaaatccg atttttccag
    11761 atttccctcg ctttgggatg gactggccgt tgaatgatac tttttcgaag gtttcttatt
    11821 gtggctatgg gccgtatgaa agttatcctg ataagcacta tgctgcgatg tttggtattt
    11881 atgagatgga gattgaggaa tttgatcagc catatatcaa accacaagaa tatggtaacc
    11941 gctggcaatg tgattgggta tcaatcaaaa atcatcaagg atctaccatg acggtttatg
    12001 gaaactctaa tttttctgct ctgccttata gtcaagctca attaaccgaa aagtcacacc
    12061 attatgaatt aataaaagaa aatagaactt ggttcaattt aagttataaa caaaatggta
    12121 tcggctcaca cagttgcggt ccttatttag aagatcgttt ccgttttatg gaagatactt
    12181 ttgactggaa tatagtgatt actttcaaag gagaaaatag atgaaagaaa caaagatcaa
    12241 tcaagtcaaa aaaaaattat ctcgttatca agaagttgtt tatcgaaacc attgcttaaa
    12301 atggtcaaaa aataagctga atgttcgttt taatggatta ttttatcgtt tcgaacagtt
    12361 agaagaagtc tggaaaccgg taacgaattt ccaatcatgt cctgtacagc tcataaatga
    12421 tccaaaagaa acatacgatt tatgtataac acacctttat aaccagcatt tgaaaaatga
    12481 cggattttat ttaaaggtta ctaatgacaa tcaacttacg attgaatcag caaaccttcg
    12541 agggtttcaa tatgctatgg aagccttatt agagagtttt ttttcggtag gagatcaatt
    12601 attattaccg gaattgattc tgaaacatga accatcagtg aaaattcgag gaatcattga
    12661 agggttttat ggtatcccct ggacacatga aatgcgcctc tctctttttt catatatgaa
    12721 agataaccaa ttgaatactt ttatgtatgc ccctaaagat gatgaattat tacgtaaaaa
    12781 atggcgtgaa ctctacgatg cgcaagaatt agacaaattt aaggagcttt tatctgctgc
    12841 tgaggctagc aatatcgatt tttggtactt aattagtcct ggaaatgata ttgatattac
    12901 gtgtgaagaa gacattcaag ttcttctgaa aaagttagaa caactgattg agcttggcgt
    12961 atttcaattt ggtctattaa tggatgatat tgactatcaa ttaaaaggtg ctgctaaacg
    13021 ccgttttcga gagccagcat tcgctcatgc ctatttagtg aatcgagtag aggaatatct
    13081 tagcactcgg ttgtccaatc atgaattagt catttgtcct acagaatatg ataatcgtca
    13141 tggttctcgc tatttagcaa cgttaagtca agaaatacca gaacaattac cgttcttttg
    13201 gactggccca agcacgttag cggcgtcaat ttctacagaa gagctgcagg aaatggcttc
    13261 tgtctatcaa cggccgatgc tgatttggga taatattccg gtaaatgatt atttagagga
    13321 taaagaacta ttgttcatga gtccatatga gaatcgaaca ccgaatcttt ctaaggagag
    13381 ataccaggtt acaggagtag tttcgaatcc aatggctcaa ttagaagctt caaagtttac
    13441 gatcaatagt atggctaatt acttatggaa ctgtgagcgt tttgatcctt tggaaacttg
    13501 gacgtcagtc gttgaaaaag tagttggaag taaactacaa ccagcttatc ttaccttaac
    13561 caatgctttt ccaaatcatt acacaagcag tctgctttca gatgaagaat tactgctagc
    13621 caaagaccca gaatgggtca caaaagaaat ggctgcttta gtccaagcag taaaatggat
    13681 ccagcaagaa caacatacat ctagattgag aacagaattg gagccttggt tacaagcaat
    13741 cacagaaagt tggactttct ggcaagaatg gcaattaaaa aaagatccaa aagaagcaga
    13801 agaatggttg gctaagaaaa aaacacaccg cataggacgt gatttagtaa ctgcccattt
    13861 agagcaaatt atcaaaagtt agatatctta aaaaataaaa agcaagaaaa aggagaacaa
    13921 aatggtcaag agattaatta gtgcaaacta cagtgatgtc aagaaaatga cgtcaaaaga
    13981 gttgaagcaa tccatcaaag cgagtgaagg acgggtgatt gtttgtgaaa cggttgctaa
    14041 tactcttcca caatctggag aaattacgaa tgcagaagtt gcgtcagcat ttggtgcaga
    14101 tttgattctt ttaaatggtt tcgattgtta tcgtcctgca gtttttggac taccaaatgt
    14161 gacgattgag aaggtttttg atctacagca aacgattgaa aacccgattc ctaaattaaa
    14221 agaactagtc ggtcgtcctg tgggaattaa tttagaaccc attgccccta aaacaaatat
    14281 gatgagtgag aaagtagaaa tcagccaagg aagagcaagc tctgttgaaa cgatacaaaa
    14341 agcagaagaa ctaggagtcg attttatttg ctttaccggt aatccaggca ctggagtaac
    14401 caatttagaa attactaaat ccgtaaaaat ggcaaaaaag tatttttcgg gattgattat
    14461 tgctgggaaa atgcatagcg ccggctcaga agagccagtt gtgaataaag aggcgattat
    14521 ttcttatgcg gaagcaggcg cagatatttt actagttcct tcagtagggt ctatcccagg
    14581 ctttttagag caagacctaa ttgaagcggt tcggttggca aaaaaatatg atttgttaac
    14641 gatgtctgca atcggtacta gtcaggaaag tgcccgacca gaaacattgc ggcaattagc
    14701 cattatgaat aaaagttgtg gtgttgattt acaacatatt ggagatgctg gatacggggg
    14761 attggcacca gcagaaaata ttttcgaaac ctcggttgca attagaggaa tgcgacatac
    14821 catcaactgc gttagccgct cgatcaatcg ctaacaaaat taaataaaaa atggttaaat
    14881 tgattattta agtgggaaca tacttgagat atcagctaac ttctgtcact ttaaaaaagg
    14941 gactgtgaca gaagtgaaaa tagacaaaaa atatccgaac aagagactag gatagcagaa
    15001 aaactatcgt tttgcaacga ggaccaactg gaccgtagga gcaattctct tattgttcgg
    15061 atatttcgct tgttcaagga cttatgtctg tagtgccctt ttttggtagg aaaaatggta
    15121 gggaaccata tcatttttaa tgaaaatcaa gcatttcaaa agt
//
LOCUS       DANKAZ010000061        14926 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000061 DANKAZ010000000
VERSION     DANKAZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            139..1476
                     /locus_tag="PWY67_001849"
     CDS             139..1476
                     /locus_tag="PWY67_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL2058113.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1524..3653
                     /locus_tag="PWY67_001850"
     CDS             1524..3653
                     /locus_tag="PWY67_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL2058114.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3628..4317
                     /locus_tag="PWY67_001851"
     CDS             3628..4317
                     /locus_tag="PWY67_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058115.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4725..5411)
                     /locus_tag="PWY67_001852"
                     /pseudo
     CDS             complement(4725..5411)
                     /locus_tag="PWY67_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5547..5741)
                     /locus_tag="PWY67_001853"
     CDS             complement(5547..5741)
                     /locus_tag="PWY67_001853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058116.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5791..6231)
                     /locus_tag="PWY67_001854"
     CDS             complement(5791..6231)
                     /locus_tag="PWY67_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL2058117.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6235..7080)
                     /locus_tag="PWY67_001855"
     CDS             complement(6235..7080)
                     /locus_tag="PWY67_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL2058118.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7229..8647
                     /gene="pepV"
                     /locus_tag="PWY67_001856"
     CDS             7229..8647
                     /gene="pepV"
                     /locus_tag="PWY67_001856"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL2058119.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8704..9651)
                     /locus_tag="PWY67_001857"
     CDS             complement(8704..9651)
                     /locus_tag="PWY67_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2058120.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9874..10224)
                     /locus_tag="PWY67_001858"
     CDS             complement(9874..10224)
                     /locus_tag="PWY67_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2058121.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10424..11503
                     /gene="pepA"
                     /locus_tag="PWY67_001859"
     CDS             10424..11503
                     /gene="pepA"
                     /locus_tag="PWY67_001859"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL2058122.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11647..11967
                     /locus_tag="PWY67_001860"
     CDS             11647..11967
                     /locus_tag="PWY67_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2058123.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11989..12453
                     /locus_tag="PWY67_001861"
     CDS             11989..12453
                     /locus_tag="PWY67_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2058124.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12658..13263
                     /locus_tag="PWY67_001862"
     CDS             12658..13263
                     /locus_tag="PWY67_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL2058125.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13302..14591)
                     /locus_tag="PWY67_001863"
     CDS             complement(13302..14591)
                     /locus_tag="PWY67_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL2058126.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
BASE COUNT         4607 a         2795 c         3068 g         4456 t
ORIGIN      
        1 gcataattaa ggttttttaa ttgaaaactg aacatttatg gtcacccttt aatgaaagcg
       61 ctaactacat aagttagaat gagtttgact gatatattag tttgcttatc agaaaaacaa
      121 aatggacgga ggaataacat gagtgatatt attattggta ttttgttagt tatctcattc
      181 tttttcatgg tctggtactg tgtaaaaggc tacaacttga tggtcggctt tgccatcatg
      241 gctactgtat ggatgggatt agcgctagtc ggaaacacgt tttcaccaaa cccggcgatg
      301 gaagggcaag gagtaattga tgtgttgacg catatctata cgacggggcc agctgagtac
      361 gccaaatcga tcttggtaaa tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat
      421 agcggaatcg cagctaccct gattcgaaag gtagttgaac ttggagggga caagccaaga
      481 atcacgatga gcttactatg tattgtaacc gctgttatat tcatgtcgat gacaggaatc
      541 ggaccagtga tttctattgc agttattgtg ttgccaatct tgatgtcatt aggtatttca
      601 gtaccggttg cactgttctc atttatgggc tcgattatgg ctggaatctt tgctaatatc
      661 gtcaatttca aacaatatca aacgatttat gctggtttca atccagcggc tgaaagctat
      721 acttataatg actatttcca aattggtatg attgggatga ttgtaagctt agttgtggta
      781 ctaacggtcg ctaatatatc aatgaacaaa aagaaacgct atgcaatggc agcaaacgta
      841 ccagctgaag gtggcgacgc accaatgatc tcatggttag ccgtattatt accagtttta
      901 ggggtcgttc ttttagatct tccaatcatc ttaggtttta ttctagcagg tatctgggca
      961 ttgctgttta ctggaaaatt gcgcggaggt tataaagaaa tctgtcgtca gtttgcgaaa
     1021 ttatttacag atggcgcagt agatgttgcg ccaatggttg gtttcttgat gaccttagcg
     1081 atgttcaata attcagctgc gtatgcttct ccttattttt cagctatttt tggagattgg
     1141 ataccgcaat caccattagt cttggcaatc gtatttgcta tattgacacc actaggattt
     1201 ttccgcggtc caatgaatct agtcggttca ggctcagcta ttttagctgt cgtattagct
     1261 gttaatccaa cgatgtcgcc agcctttttg ttcccgttat ttgcaatcac aacgattgct
     1321 cctcagcatt tagatattac acaatcttgg gttgcttggg gattaggtta tacaaaagta
     1381 acttctcgag aatatatgaa gaaatccatc ccaactggtt ggatcatcgg agcgatctta
     1441 tgcctgatca cgttcttatt gtacgggaac gcctagtaaa caaaacgtcg ataaaaacga
     1501 ttagatgaat ggaggaaaag aaaatgaaac gttttgtgcg aatgggaatt gatgttggcg
     1561 gtacacatac gaaagcagtc gcaatcgata atgcgacgca cgaaatcatc ggaaaatctt
     1621 cagtgaaaac aacccacgat gatgtccgtg gagtagccgc aggtgtcgtt cagtcttttc
     1681 aaaattgttt gctggaaaat aatatcagtc cagaagatgt tgtgtttgtt gcacattcta
     1741 ccacacaagc gactaatgca ctgattgaag gagacgtagc aaaagtcggc gtaatcggga
     1801 tggcaaaagg tggattagaa ggttttttag caaaacgaca aactagattg aacgatatcg
     1861 atttagggaa taagaaaaaa atagagattg tcaatgcatt cttacctgtc aaacacttaa
     1921 atgtagaccg tgtttcagaa acgatttctt ctttagaaag agaaagagcg gaggtcttgg
     1981 tttcttctat ggcatttgga gtagataatg gggaaccaga acgagttgtt tatgaagcag
     2041 catcggtaaa atcgatccct acaacgatgg cttctgacat cacaaaatta tatggattga
     2101 caagaagaac tcggacagca gcaatcaatg catctatttt accaaaaatg ctagatacgg
     2161 caacctctac agaagattcc gtacgagaag caggggtcaa tgtttctttg atgattatgc
     2221 gcggagacgg cggagtaatg gaaatcaatg aaatgaaaaa acgacctgtt ttaaccatgc
     2281 tttccggtcc agcagcatca gtcatgggct ctttgatgta cttgagagcg tctaatggtg
     2341 tttattttga agtaggcgga acaacaacca atatcggggt cattaaaaat ggacgtccag
     2401 caatcgatta ttcaattgta ggtggccacc caacatatat ctcttcatta gatgttcggg
     2461 ttctaggtgt ggcaggcggt tcgatggtac gggctaatca gtcaggaatc atcgatgtcg
     2521 gtccacgttc tgcacatatt gccggtctag attatgcagt atttacggag acagaaaaaa
     2581 tcaaaggtcc taaggttgaa tttttctctc ctaaagaagg agaccctgct gattacgtca
     2641 aggtagtgat ggaagacgga gaagaagtga ctattacaaa tacatgtgca gccaatgtcc
     2701 ttggattagt gcaagaagaa catttttctt atggaaatgt cccatctgca cgaaaagcca
     2761 tccaagcatt agctgattat tgtcatacga cggttgaaga catcgcagaa cagatcatgg
     2821 aaaaatccta tgctaagatt gaaccggtga ttttagaatt ggcagacaag tatcacttgg
     2881 agaaagatca gatttctctt gtaggtgtag ggggcggcgc tgcctcattg attacttact
     2941 ttagtaataa aatgggtgtg aaatattcga ttcctgaaaa cgccgaagtg atttcttcta
     3001 ttggtgtagc acttgcaatg gtccgagatg ttgtagaacg tatcatccct tcgccaagta
     3061 aagaagatat acgatcgttg aaaaatgaag cgatgaataa agcgattgaa tcaggcgcta
     3121 caccagaatc gatcgaagtc cacgtagaaa tcgatccaca gacttcaaaa gtaacggcta
     3181 tcgctactgg ttctacggaa gtgaaagcaa ccgatctaac caaggagatc acaacagaag
     3241 aagcactaga gcttgctgca gaggatatgc gattaaataa aaatgaagtt tgcttattgg
     3301 aaaatacacc tttcttttat gtatgcggcg aacagaatcg ttcaaaaaat gctggaagtc
     3361 ttcggatcat cgatcaaaaa ggatttatca aggtccaacg tggacatgct tcatgtatga
     3421 aaacaactgc agccaattat atgacagctg tcgaacaact ttgggaagat atggcagttt
     3481 atcaaactga attgattgcc cgaccagaat tttatctctg cttaggagca agagtctctg
     3541 attttacagc gacggatctg gaacaactac agcttttgat ggatctggaa gtatctacta
     3601 tggaaccgga ggaagaagtg atcgtcgttg ccggaaatat caaacaaaca taagttagaa
     3661 gagttactaa acgaattaca acagatcccc gaagaaattt ggggattcta ccagtttcgg
     3721 cgagatttat tttggaaaaa gattcctttg tccaagcaga aaattcttat acaacaatca
     3781 atcgattgtg gtatcgaaac ggcatgtagc atcaaaaaaa agtacccttt tgccgatgta
     3841 ggagaaattt gtgaacaaat ggctgttcca atcgtttcct gtgaatcaga gcaaataaac
     3901 gaacgaatca cttttgcaac ttatgcagaa gatgaaggga ttcgattgat gacggagccg
     3961 ttagaaaaat taaaatgttc aggactagct tcgatctcta aagaaacagc gcaagctttg
     4021 attattggac atgaactatt tcaccacatc gaagcttctg ttaagggaat ttatacgcaa
     4081 agcgaaaaaa ttgtgctgtg gcggttgcct ttttatacac atcaatcaaa cattcgtgca
     4141 ttgagcgaga tagcagcgat gagtttttca aaagagatga atcaaagcga tttttcgcct
     4201 tatgtattag aggcagtctt actttggcca tataatgaaa ttcgtagcca agggatctta
     4261 gaagaaataa aaaaaataga aaaaagatgt acggagtatg atttagatta caaataaaga
     4321 gactgtgaca aaagtcgtgt cacagtcctt tttttccgaa taaacggtgt tctcctgtca
     4381 actcctcggt cacaagtcaa aatattcaac aaacatgaga agctgttttt tgaaattgtt
     4441 ccttgtgact cggagatgaa cggcaggctc acgacctctt ggaaaacggt gttcaaaagc
     4501 ttgcatctgc ggtaataagt ccagccaagt taaaaacata agaagctatt tttacttggc
     4561 tgttcttatt acttgatgca gggcagcttt ttcacaacct ctcaataaac ggtgtgcaat
     4621 cagctgaccc tcggtattag ctcaaaaaaa acggcaaaat atgaggaact attttgccgt
     4681 ttctttctct aatactcagg tcaaaacgct gattccaaaa cctcttattt gatgggtctg
     4741 tcctgaatat cttcaaaata atcggggtaa ttatgaatca cgtattcttg ctcctcgata
     4801 atcatatgta aatgcatcgt gcaaagaaat ccgacttcat caaagttatg atcaatcatt
     4861 gattgaagca ttgtctgatg ttcatctaat actcgacccc agtctaattc tgaaatcgtc
     4921 aaacgtaacc atctgaaacg gttcaagtgc gtactataac tttctagcca atcccaaatt
     4981 tctcgttttc cagcaatttc ataacaagtt cggtgaaaat cagtatccaa atcaaaaaaa
     5041 gcttttttat tttgagcgat tactgcctga tcggtttgtt ctagtatttt ttctagttta
     5101 gcttgatctt acttggtcat tttcgcactg cattcttgca ggatcggttt ttccaacttt
     5161 tcccgcgtat aacgggctaa tgaagcagat cggatatcga tttttgaaac ataagtgccg
     5221 gactgtggaa tggtttttac aagctcttga ttgcgcagtt ggatcagtgc ttctcgaatc
     5281 ggtgtacgcc cgatagctag ttgttcttct aacactttat cggagatttt acttccaggt
     5341 gctaactcgg aataaatgat ttgtttacgt atagattcat aagcttgctg ttgcaaatta
     5401 ttgataacca tttaaccttc ctttctatgc agatataatc tattataaca taaatcgctt
     5461 acaaatcgtg tgggcataaa aacacagtct gcaaaaaaag ccctgatagg catgcctatt
     5521 agagcctcga aaagaaaaat attgaatcaa tctggtcgta cctcttggat cggtttgaca
     5581 cgaccgccga ttcttttagc aaaagcttcg gattcttttt tgtctttgaa aatgagcaga
     5641 ttgtctaagt ttttttcccg agtgcgacag ccagttttat cgatgtaacc gtgcattact
     5701 ttaacgacat acatggggat cacctgcttt tttcttttca ttatacgaac tactcgacac
     5761 aatacaagaa aaatgcttag acggtcttta ttaagaattt acatcatcca aagaagcgta
     5821 cgcttggaat aactgatcta actccggatt gactttgctc agcttcacta atcgttgcga
     5881 atcagaaagt aaaaaacagt gtttactact gccagtagtt tttaattttt tgtcttcagt
     5941 tcccacaatt tcgtagctaa aatccaaccg tgtccccgta tacttttgga tagttggatg
     6001 gatgctcact ttttctccgt aatggatcat ttctttatag ctgcaattta cttccagaac
     6061 ggggatgatg atcccagatt cttcgatttt tttataggga aaccctagat gtaccaataa
     6121 ggctacccga gcctcttcaa accagcggat ataattgctg tgatgaataa tccccatttg
     6181 atctgtttca taataaaaag gttctcgcac ataacctgta aatttctcca tgactcatgc
     6241 ctcgttttcc attgattttt tacaaaaata attcatccat ttcggaagtg cttcgctgat
     6301 ttgtgtcggc aaaacaatat attgttcttt tgccagctgg tctccaatga aactatgaag
     6361 ataaatagct gctgcaatcg tttggtcatt ttgaggaaac tgggctaaaa atcctgtgat
     6421 catcccagct aaagtgtcac ccatgccgcc cgttgccata gcagcagaac ctaaaggatt
     6481 ggtgtagaag tgctggtgac cataaatttt tgtctcatga cttttcaaaa cgagcacagc
     6541 accgatttcc tgttgcttct ttttgttttc tttatcccgt tgttcttgta tcggtagaga
     6601 actcaaccgt tgccactcca tttgatgagg tgtaagtaca gtttgttcgg gatagggtag
     6661 caaaagatga ttagcagcaa gtaaacttaa agcagagcca tcaatcacca gccattgttc
     6721 ttttgattgg ttttctagta cttgtttgaa acgttccaaa ctcttagaag aagtacccaa
     6781 tccgggacca atcaagagta catctgcttg ctttattacg gaaagaaact ggtcagtatc
     6841 cgtccaaact acaatcattg cttctggcag atgactgtga agagcactcc gatttttttc
     6901 atcagttact acagtcgtga gacccgctcc gctgtgtacg gcagctaaag cactcataat
     6961 gattgctccg ccatattgtt cattgccgcc aatcaaaacg atacgtccaa atgttccttt
     7021 atgtgaaatt tttggacgag gtgtgatgac tcttttcact agttgttcgt ttatcagcat
     7081 ctaaaaacct ccttttactc attatagcgt atattttttt tagagaaaaa cagataagaa
     7141 aaggaattgt cagatttttt gaactatttt tttctgtaat ttgtggtatc atgaacacga
     7201 agacttaaat aagtggagga ttaactttat gacaattgat tggcaaaaag aagtagaagc
     7261 acgtaaagaa gacttgcttg aagacttgaa aaatctttta cgtgtcaaca gcgagcgcga
     7321 tgactcaaaa gtaacaccag atgcaccatt tggacctgga cctcgcgacg cattgaaaca
     7381 tatgttagct tatggcgaac gtgatggatt taaagtaaaa aatgtcgata actatgcagg
     7441 gcatattgat cttggcgaag gagacgaaac gctagggata tttggacaca tggatgtcgt
     7501 tcctgcagga gatggctggg acacagatcc ttatgaacca gtaatcaagg atggaaagat
     7561 ctttgcacgc ggatcaagcg atgacaaagg tccaagtatg gcagcttact atgcgatgaa
     7621 gatcattaaa gaacttgact tgaaactatc aaaaaaagtt cgcttcgtag tcggcagcga
     7681 tgaagaaagc ggctggggcg atatggctta ttatttcgaa catgaagaag aaccagattt
     7741 tggtttctca ccagatgctg aattcccaat catcaatggt gaaaaaggaa atgtttcttt
     7801 agctttacgc ttcaaaggcg acaatgcagg agattatgtc ttgaaatctt ttgtttccgg
     7861 acttcgcgaa aatatggtgc caggaacagc aactgcagca cttcaagtac caagtgcaga
     7921 tgcagctatc gcgatggaag aagcatttta ccaattcatc gaagcaaatc cagtaagcgg
     7981 aacgattgaa gcggacaata cgtatgtaaa aatcgaatta gttggtaaag gcgcacatgg
     8041 tgcaagccca caatctggta tcaatgcagg ttctttcttg gcattgttct tagataacta
     8101 tgaattccta ggatctgcta aacaatttat ccatgtagca gctgcttatg tccatgaaga
     8161 tttttatgga gaaaaactag gagtcgctta tgaagatgaa aaaatgggca aattgacaat
     8221 gaatgctgga ctatttgctt ttgaagaaaa tggtacagag gaagcgaact tcatcaatat
     8281 gaatttccgt tttccaaaag gtgtaacagt agacggattg caaagtgaca tcgaacaaac
     8341 agtggggcaa gaaggcgcaa cagttactcg aggtgctcgc gtaatggaac cacattacgt
     8401 accaatggat gatccattgg tggctacatt attgcaagtc tatgaagacc acactggaga
     8461 aaaaggctac gaacaaatca tcggtggcgg aacttacgga cgtttgttga aacgtggcgt
     8521 tgcttatgga gcgatgttcc caggatatac tgatacaatg catcaagcaa acgaatttat
     8581 gagtcttgat gacttgttca gagcaacagc aatctatgca gatgccatct atcgtttggc
     8641 gaaataatta attaaaaaga ggatcaagaa ttgcaaatta tttttgcagt cttgatcctt
     8701 tttttacttt ctactgtggt attcatgata aacatgatat agattcaatt catattcttt
     8761 cttagcgttc gtgacgactg tatccaaaat caaccctgtt actaatgaaa gaagtgctaa
     8821 agtcatcaat ccagtactta aaatcgcaga agggatgcgt gtaataaagc ttgtcagcat
     8881 atattcgcga atcacaggaa tccccgtgac caatccaaat aaaaagaaga agatggtcca
     8941 aatcccaaag aaaagcaaag gcttataatc tttaaacatt ttaataatca tcatgatcac
     9001 tttgaatccg tcagagaatg tattcaattt tgattcgctg ccttcaggac gatcacgata
     9061 atcgattggg atttcaacga acttgaattt tttatctaaa gcatggatcg tcaattccgt
     9121 ttcgatttga aaaccagaac tcataatagg aaaacttttg acaaagatac gattgaatcc
     9181 tcgatagccg gtcatgacat caagaatttt tgttttatac attctagtaa ttgtatttct
     9241 aacaaggtta tttccaaaat cgtggaaagg tctcttattc tcatcaaagt aggttccatt
     9301 tgataggcga tcaccaatga ccatatctgc ttcaccagaa cgtaatttct cgagcaaacc
     9361 atgtacagct tctgcaggat aagtatcatc gccatctacc attaaataat agtcggcatc
     9421 gatatcaaag aacatttggc ggatcacatt tccttttcct tgacgaggtt ctttttttac
     9481 gattgctcca ccttgaacgg ccaattcgta tgttcgatcg gtcgaattat tatcatagac
     9541 ataaatatct gcttctggta attctcgttt aaagtctgca atgactgttg aaattgttgc
     9601 ttcctcattg taacaaggaa tcagaacagc aattttttct tgttgttcca tttttcactc
     9661 tccttaacta tagaaaacaa aagctaaatt aaaaatacct cataatcaaa ggaaatgcta
     9721 gtttttcccg ctaaatatca tttatttaaa aagaaaatac tggaatatat gtttggaaag
     9781 ctctatctaa aaaaccaccc actacaaaag aaggacatga tacctctctt ttgaatagtg
     9841 ggtgcgtatt cgttctctca cttttttgac gtattaatct gttgcacctt tttttcttga
     9901 aatttctaat acggttcctg tatgagcatc tgctaaaaat tcataataaa cagtttcgcc
     9961 atcttctatt cgagaaagac ctccccgata agcactcgac cgaacggcga atttcctaat
    10021 aggttccttt tcaaaagaga tccaagaacc ttcgattgca ccttctttta gaaaagatga
    10081 cttgatattt tcaagtactt ggtctgctga caatactttt ttgcgatgat accaaatcgt
    10141 tgaagctacc ccgctcaata atcctagacc gattccaact gctaacccgc ctttaaaata
    10201 gcttagttcg ctttcttcat acatgtggac atacctcctt cttctcactt gttactcctt
    10261 gtgcttattt taccacactc tcatattttc tttgaaataa ataaccgcta ccagaaaaaa
    10321 taagctataa taaatacgat cggtctgcag aaaaaatcat attttatccg cagatcaata
    10381 taagtctgag aggagtttag tctgaaattt agacgaatag catatggaag aaaaaacatt
    10441 tcaacgaatc aaagagttaa cagaattaca aggaacaagc ggttttgaac aagatgttcg
    10501 cgcttatatg gaaagtcata tggaaccgct ggtagatgag ttacagttag atggtttggg
    10561 cggcattttt ggtctgcgcc atcatgaaga agcggatgca ccccgtgtaa tggtcgcagc
    10621 acatatggat gaagtcggat tcatgctgac acaaatccaa gaaaacgggc tttttaaagt
    10681 cgtacctcta ggcggctgga atccttacgt tgtttctgct cagcgtttta cacttaaaac
    10741 ctctactggt aagaattatc catgtatttc ttcttccgtt cccccacatt tattgcgagg
    10801 aacaagcgga caaaaatcag tagaagtaac cgatattttg ttcgatgctg ggtttgaatc
    10861 aagagaagag gcaatgagtt acggtgtcct tccaggagac acaatcgttc cttacgctga
    10921 aacgatcaaa acagccaacg gaaagaatat tatcagtaaa tcatgggaca accgctatgg
    10981 ctgtacaatg gtattagaag ctttagaagc gttgcaaaat gaaacattag gtcatacatt
    11041 gattgcaggc gcgaatgtcc aggaagaagt aggcttgcgt ggttctaaag cttctgtcaa
    11101 taaattcaag ccagatctgt tctttgcagt agactgctct gcagcagatg atacagtgac
    11161 gaaaaatggc acattcggac atttaggaga aggtacattg atgcgtatcc aagatccagg
    11221 tttgatcatg cttcctcgtt tgcgcgagta cttgctggat atcgctgaaa caaacaatat
    11281 cccttatcaa tactttgttt caaaaggcgg tacagatgca ggtgctgctc atacacaaaa
    11341 cgaaggaatc ccaagtacag tgattggcgt agtgggacgt tatatccata cgcatcaaac
    11401 gatgttcagt attcgcgatt ttgaagctgc acgagaaatg ttgattcaaa cactgaaagg
    11461 tttggataaa tcaacagtaa atacgatcgt atacggaaaa taatgcgata gtgaaactat
    11521 aaaatctggc aaaagattgc tttttgccag atttttttta taaaaagagg atcaatgttt
    11581 gttagttcga aagaaaggtg tataatttaa ttgaaaaata cataaacaga aagtagagga
    11641 caaagaatga ttatcccaaa tagtttagaa gaattagcag gctacgtaga aactggaaag
    11701 agtgtatttt tctttactgc cggctggtgc ggagactgcc gttttatcaa accgcaaatg
    11761 cctgaaattg aaaatgactt ttctgattgg caatttattg aagtagaccg tgacaagtac
    11821 atagatgtag ccgctgaatg gaacattttt ggtatcccaa gttttgtcgt gatccaagac
    11881 ggaaaagaat taggtcgtct agtgaataaa gatcgtaaaa caaaacagga aatcgaatcg
    11941 ttcctagaaa gtgtaacaaa aggctgacat ctgttaggag gggtcaagat gaatcaagaa
    12001 tataagaata ttttagtagg tatcgacggt agtgaacaag cacttgctgc tttcaaaaaa
    12061 gcggtagaag tggctcgccg taataatggg aaagtatatg tagcaaacgt aatcgatcag
    12121 caattttata actttatggg ctatgcacct attgatcaaa atctagtcga tcagcaaact
    12181 gaaacagcca aggaaatgat cgaagaatgt aaagaatatg ggaaatctgt tgattacaca
    12241 aatatagaag gaattattgc atatggttct gttcgtgaat ccatggcagt caaattgcca
    12301 aaaaaatatg agatcgatct aatcatggtt gggcagtcag gtctaaatgc agtcgaacgc
    12361 tttatgacag gaagtgtagc cagctacgtg atacgtcatt caccttgtga tgtcttgatc
    12421 gtttctgacg aagaagaaca gccaagtaat taagcaagtg gaacttgtac gaccttttta
    12481 atcaattaac taaaacagta aagggactgt gacaaaagtc ttgtcacagt cccttgttac
    12541 gaataaatgg tgttccagaa gaggcttctt cggaaataaa gcatgaaaaa aatttcatgc
    12601 agtgaatgca attttttgtt aagataaatc tcagcaaagg ataatggagg aaagaacatg
    12661 atttttgctt ataacaaagc acacgtcgga gacaccttga tggttattat agccgacgat
    12721 aaaggaacag aaaacactgt tgaacgcaaa tggaatgttg ctcggatcaa agatgaacaa
    12781 ggtcaagttg ttgcttggaa ctttttccat atctccgatc atctaacaat cgaagggaac
    12841 ggccaagtaa gcttaacaga tgaggaatta gcgaaattga atcgattgat caaagaagca
    12901 ggattcgaag aacagcttga agctgatcat gaaccgaaga tcgttgtcgg gtacgtaaaa
    12961 acatgcgtcc cacatcctga ttctgaccat ttatcgatta ctgagacaga agtagacaat
    13021 ggtacagtat tgcaaatcgt ttgcggtgca cccaatatcg aagctggaca aaaagttgtc
    13081 gttgcaaaac caggagctat gatgccagat ggaatgatga tctggccagg aactttgcga
    13141 ggggtagata gctatggaat gatctgttcc gctcgtgaat tgcatttgcc aaatgctcca
    13201 gcaaaaaaag gaatcttagt attgcctgag gatgccgtag tcggagaagc atttccaaaa
    13261 taaagaggtt gtgacaagtc tttgtcacaa cctctttttt attaattttc actattttga
    13321 ttttgtttta atgccgattg atcgatcgtc aattcaacag tagttgtttt ttctttcgag
    13381 ccttcatagt aagtgatttt gacagaatct cccacttttt tcttatacaa agcagattga
    13441 agttctacac cagaagaaac ttctttgtca tcgatcttag taatgacgtc gtattgtttc
    13501 aatccagctt tttcagccgg tgtagctgtt tggacatttg tcacgatgac accgttagtt
    13561 acagatgaag gaatcttcaa gatttgttct tgttgctgag tagaaacggc agataagtcg
    13621 accattgtga tacctagagc tggacgtgtc acttttccgt ctttttccag ttgattgatg
    13681 atattcacta catcgttact tggaatagca aatcccattc cttctacgct tacattagaa
    13741 gtagattcag aagtacttgc gatcttactt gagttgatac cgataacttg tccttcgata
    13801 ttaactaaag gaccaccaga atttccaggg ttgattgctg catctgtttg gatcgcattg
    13861 atattgactg tttcgttcga ttcattcgta cttgtcactt gtcggttcaa ggaagaaatg
    13921 attcctgaag tgacagaatt tgcatattca gatcctagtg gggaaccaat cgcaatcgca
    13981 ggctcaccaa ccttcagagc acttgagtca ccaaaagagg caactgtttc gactttgtct
    14041 gaattgatct tcaacaccgc taaatctgaa taagcatctg ttccaactaa ttcagctttt
    14101 accttcgtac cgtctttcat caagacttca agaccttgct ggccatctac tacgtgatta
    14161 ttcgtgacta cataagcagt attcccgtct tttttgtaga tcacgccgct tccttcacta
    14221 taagcttcca aattactatc atctgagctg ctactttctt cttgctgccc gaatagtccg
    14281 ccaaaaccgc ttgattggtt ttgactttgt aagttgataa cagaaacgac agcgtcttga
    14341 actttatcta ccgctttggt gatatcagaa tctacattga ctttgacatt ttccacaact
    14401 gtttcaccgg cagaattctg attgccgctt gtagcagtag aactattgtt gccagtgccc
    14461 atcgctgcat aaaaaatgcc agctgtcagt agtcctccaa cgatccctcc aacaagcccc
    14521 agacctaact tacgccagag gctattattt ttactttttt tcatctttgg tgtcacattt
    14581 tttctatcca tgtgttcctc ctccaattac attcatttat ctactgttag tatagtctta
    14641 tttgttgact ttagtctact ttgagactgt gaacttttta tgaaaaactt taggaaaaat
    14701 cattgttttc agatgaaaat atttgatttt ctttttccac agatactgtg taaaactacg
    14761 cggataggat tggaaaaata agggataaaa catatccaca gcctgtgtat aaagtggata
    14821 aattcttata aaacgtatgt ttgcctaatg aatgtgatga aataaggtaa aataacactg
    14881 ttttctgtgg ataaacctgt ggatgttgtg cataactgtt ggggaa
//
LOCUS       DANKAZ010000062        14586 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000062 DANKAZ010000000
VERSION     DANKAZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..626)
                     /locus_tag="PWY67_001864"
     CDS             complement(<1..626)
                     /locus_tag="PWY67_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2058127.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="PWY67_001865"
     CDS             899..3370
                     /locus_tag="PWY67_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2058128.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="PWY67_001866"
     CDS             complement(3473..5209)
                     /locus_tag="PWY67_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL2058129.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="PWY67_001867"
     CDS             complement(5206..5910)
                     /locus_tag="PWY67_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2058130.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="PWY67_001868"
     CDS             6041..6544
                     /locus_tag="PWY67_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL2058131.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="PWY67_001869"
     CDS             6504..6842
                     /locus_tag="PWY67_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL2058132.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="PWY67_001870"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="PWY67_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL2058133.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="PWY67_001871"
     CDS             8393..8971
                     /locus_tag="PWY67_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL2058134.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="PWY67_001872"
     CDS             8975..9496
                     /locus_tag="PWY67_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2058135.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10129)
                     /locus_tag="PWY67_001873"
     CDS             complement(9575..10129)
                     /locus_tag="PWY67_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2058136.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10382..10657
                     /gene="rpsP"
                     /locus_tag="PWY67_001874"
     CDS             10382..10657
                     /gene="rpsP"
                     /locus_tag="PWY67_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL2058137.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10669..10920
                     /locus_tag="PWY67_001875"
     CDS             10669..10920
                     /locus_tag="PWY67_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL2058138.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11045..11836
                     /locus_tag="PWY67_001876"
     CDS             11045..11836
                     /locus_tag="PWY67_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058139.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12006..12527
                     /gene="rimM"
                     /locus_tag="PWY67_001877"
     CDS             12006..12527
                     /gene="rimM"
                     /locus_tag="PWY67_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL2058140.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12517..13278
                     /gene="trmD"
                     /locus_tag="PWY67_001878"
     CDS             12517..13278
                     /gene="trmD"
                     /locus_tag="PWY67_001878"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL2058141.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13414..13761
                     /gene="rplS"
                     /locus_tag="PWY67_001879"
     CDS             13414..13761
                     /gene="rplS"
                     /locus_tag="PWY67_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL2058142.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13893..>14586)
                     /locus_tag="PWY67_001880"
     CDS             complement(13893..>14586)
                     /locus_tag="PWY67_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2058143.1"
                     /translation="SYAMKKGYVEKNPINEVVIDYKRESKTIKIKDKFLEDDEYKRLV
                     NFTTSHNKRYSLLFQWLYLTGMRPGEAIALSKDDVHITNNNASVVINGTMMYRERSIA
                     DMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTTKGTPFQLTAINTYL
                     RNHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHENSDITEKIYLHVTKG
                     VKEKLKEDIEKL"
BASE COUNT         4757 a         2666 c         2967 g         4196 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10141 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10201 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10261 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10321 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10381 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10441 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10501 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10561 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10621 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10681 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10741 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10801 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10861 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10921 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10981 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    11041 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    11101 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11161 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11221 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11281 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11341 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11401 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11461 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11521 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11581 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11641 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11701 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11761 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11821 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11881 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11941 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    12001 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    12061 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12121 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12181 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12241 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12301 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12361 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12421 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12481 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12541 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12601 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12661 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12721 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12781 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12841 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12901 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12961 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    13021 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    13081 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13141 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13201 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13261 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13321 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13381 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13441 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13501 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13561 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13621 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13681 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13741 atcaaagaaa tccgtcgtta atcatcgtta tcagactgag aaagaaccct tggtattcaa
    13801 gggttctttt tttgttctct gtttttttat attaacgatt tctatttggg acatttttga
    13861 ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
    13921 ttttctttta ctccctttgt cacatgcaaa taaatctttt cagtgatatc actattttcg
    13981 tgcccaacgc gatcctgaat agcatacaga ggagttccta gttccgctaa ttttgaaata
    14041 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
    14101 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
    14161 agaaattgac cattcggatt taattctaga agctcattgt aaatagctat cgcttttttt
    14221 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
    14281 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc attattattg
    14341 gtaatgtgta catcgtcttt acttaatgcg attgcttctc caggcctcat tccagtcaaa
    14401 tatagccact ggaaaaggag agaatatctt ttattgtgtg aagttgtgaa atttactagt
    14461 cttttatatt catcatcttc taaaaactta tctttaattt ttatcgtttt tgactctcgt
    14521 ttataatcaa taactacttc gttgatagga tttttttcta catagccttt tttcatagcg
    14581 tatgaa
//
LOCUS       DANKAZ010000063        14525 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000063 DANKAZ010000000
VERSION     DANKAZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(330..692)
                     /locus_tag="PWY67_001881"
     CDS             complement(330..692)
                     /locus_tag="PWY67_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058144.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(766..1146)
                     /locus_tag="PWY67_001882"
     CDS             complement(766..1146)
                     /locus_tag="PWY67_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL2058145.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1210..2403
                     /locus_tag="PWY67_001883"
     CDS             1210..2403
                     /locus_tag="PWY67_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2058146.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2480..3064)
                     /locus_tag="PWY67_001884"
     CDS             complement(2480..3064)
                     /locus_tag="PWY67_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2058147.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3265..4263
                     /locus_tag="PWY67_001885"
     CDS             3265..4263
                     /locus_tag="PWY67_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2058148.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4375..4884
                     /locus_tag="PWY67_001886"
     CDS             4375..4884
                     /locus_tag="PWY67_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL2058149.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4914..5573)
                     /locus_tag="PWY67_001887"
     CDS             complement(4914..5573)
                     /locus_tag="PWY67_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL2058150.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5575..6339)
                     /locus_tag="PWY67_001888"
     CDS             complement(5575..6339)
                     /locus_tag="PWY67_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL2058151.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6347..7024)
                     /locus_tag="PWY67_001889"
     CDS             complement(6347..7024)
                     /locus_tag="PWY67_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL2058152.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7061..8446)
                     /locus_tag="PWY67_001890"
     CDS             complement(7061..8446)
                     /locus_tag="PWY67_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2058153.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8656..8740)
                     /gene="ffs"
                     /locus_tag="PWY67_001891"
     ncRNA           complement(8656..8740)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PWY67_001891"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="PWY67_001892"
     CDS             complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="PWY67_001892"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL2058154.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9385..10242
                     /locus_tag="PWY67_001893"
     CDS             9385..10242
                     /locus_tag="PWY67_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2058155.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10323..10658)
                     /locus_tag="PWY67_001894"
     CDS             complement(10323..10658)
                     /locus_tag="PWY67_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058156.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10725..12095)
                     /locus_tag="PWY67_001895"
     CDS             complement(10725..12095)
                     /locus_tag="PWY67_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL2058157.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12173..12709)
                     /locus_tag="PWY67_001896"
     CDS             complement(12173..12709)
                     /locus_tag="PWY67_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2058158.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13018..14499
                     /locus_tag="PWY67_001897"
     CDS             13018..14499
                     /locus_tag="PWY67_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2058159.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4475 a         2891 c         2358 g         4801 t
ORIGIN      
        1 actcagttat cttatcatgt cgttagtaac atgtcaataa ctatttttta aattttttag
       61 aaatgtgata taacatccac ttcagaatca acgctgttaa atttatcatg tatttcttcc
      121 ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa ttattattat
      181 tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta
      241 gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt
      301 caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa
      361 gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct
      421 tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata
      481 tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac tatttctttt
      541 aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc taacgcaaat
      601 tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc caaatgcaac
      661 ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa cttgttctat
      721 ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt tcttattttc
      781 tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct ccgcaatagt
      841 ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat atggaacagc
      901 tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat attcatcaat
      961 gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga tatttttcgt
     1021 ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat caagagaaac
     1081 gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc caattttata
     1141 agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc aaaaaaagaa
     1201 gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat ttcaagaaaa
     1261 gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga tgacttactt
     1321 cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa agcgtttagt
     1381 gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact atatcaggct
     1441 atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa tatcgttttt
     1501 acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa accaggcgat
     1561 tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga aacaaatcaa
     1621 cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat ggacttgtct
     1681 gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg caatcctcac
     1741 aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga attgtgttta
     1801 aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt attcgatcat
     1861 acatttactt ccatgctaac gattgatcct aatttagatg atctactagt tgcttttact
     1921 tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt tatcaaaaat
     1981 ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca cgaaatcaac
     2041 acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg gttaagccaa
     2101 ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga acagtatctt
     2161 ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga ttttaccgcc
     2221 tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa agtcgtatta
     2281 aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa tattgcttgt
     2341 ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga gacaatcgat
     2401 taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct ttttcataat
     2461 tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact acatcatgta
     2521 caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg tccatccctt
     2581 ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg ccacctttat
     2641 acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc atattagctg
     2701 gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg
     2761 ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct tcaaaacttt
     2821 cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct tggatcatga
     2881 aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg gaaagttcaa
     2941 tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta atgtcaccac
     3001 ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt tgtgggaatg
     3061 ccatattgtt tcctcctaat atttgcatac ttcataataa taataacata atacctaacc
     3121 tctcgtaaaa atactagatt tgttttcttt tatttttcat cactagatac attctataac
     3181 ttaccgtttc gatttcgaat tcaaacattt ttgagaagca ttctcaatta aattctgcta
     3241 tagtgtgaaa agggaggaat ttttatggaa atctatgaca ttacaatcat cggtgcaggt
     3301 cctgtaggga tgtttgccgc tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt
     3361 gacagtctac ctcaacttgg tggtcaatta gcaactttgt atcccgaaaa atatatttac
     3421 gatattcctg ggtatccagc aataaaagca agtgaattaa tcgatcaatt agaaaaacaa
     3481 ttaactactt ttaaccatac attccattta aaagaagaag ttctctcgct tactcgtgaa
     3541 gacgaagtta tcgagattac tacaaataaa ggcatccatt attccaaagc agtcatttta
     3601 gctttaggaa atggttcatt ccagccaaga aaattgaatt tagataatgc tgaatcattt
     3661 gaaaaccacg gattggatta tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc
     3721 gctattgctg gaggtggtga ttctgctatc gattggacgt tgatgttaga gcctattgca
     3781 agcgaagttt acttgattca tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca
     3841 cgcttgaaat cttcatctgt caacttgctt actccctatc tcatcgatgg attatcaggc
     3901 aataatgggg aactgacaga tatccggtta aaaaaagtga aaagtgacga gacgattgat
     3961 ttgatgattg attctctgat cgtcaattat ggattctctt ctaatttaga acatctttct
     4021 tcttggggac ttgatagtac cagaaatgcc attactgtaa aatcagatat gtctacttct
     4081 attccaggcg tttatgcagc tggtgacatc tgtacctatg aaggaaaagt gaaactgatt
     4141 gctactgggc tcggtgaagc acctactgca gtgaacaacg ctttacacta tattaatcca
     4201 aaggaacgaa ctcagcctgg tcacagcaca agcttatacg ataaaaatct tcgtcctagt
     4261 tgatttttta tatctctttg catttcaagt taaacccgaa agcagtttct tgcatttttt
     4321 tcatgttccg ttaacatgaa atagagaaag aaaaaataaa gggggagcaa taatatggtc
     4381 gtcaaaactg agactttaga agaaaaggca agagaactat tgaaggaacg tggcgtaact
     4441 gtagaagata tcgcacaact tgtcatgttt ttacaaaaag actatatttc tgatttgact
     4501 ttggcggatt gtactgaaag tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc
     4561 attatcactg gaatccaatt agatatatta gcagaaaaga aaaaattgat gagtccgttg
     4621 caacatatta tcgaagatga cgaaggcctt tacggtatcg atgagatcct agccttgtca
     4681 atcgtgaatg tttacggtac gatcggcttt acgaattatg gttatatcga caaggtgaaa
     4741 cctggtattt taaaagaact aaacagccac gatggcgttc atgtccatac cttcttagat
     4801 gatatcgtcg gagcgatcgc cgctgctgct gccagtcgat tagcacacaa agaaccagaa
     4861 aaaagaagtc gccttactca ataattcaac aaaaaaagaa agaaaacgtc tatttagacg
     4921 agttctttct tttttgtttg tttcaagcta ttttttccta agaaaagaaa aacagcaaaa
     4981 aataattcta ataatacaaa aaataagata aagctatgca tgaaaaattc ttctggaagt
     5041 acattgatga ttggatcagt aacgggatcg aataaccagt catcgttgtt gaaaaacgtt
     5101 tcatgaaaaa ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact
     5161 ggcagtaaca tgccgatttg aaacggacga atcaaccgcc aaagccgatc attcttttgc
     5221 aaatatttga taaacaaaat actcgggatc aaagtgatga aaaacacagc ataatccact
     5281 aaaaacaaaa ttttcacttc ataaaagtga tgggctccgc tagcagaaac tgggaaatct
     5341 ggcaagctga ggatggtttt gaagggatta ttgagataat tcattaggtg atcaaaattt
     5401 ttcagtaacg tctcttgatc taaagaagta taatccaaaa tttgcaaatg tccgatatca
     5461 aatacgtata gtggtcgaaa gttgatcgtc acaccaactg ccagactgat cagtgtcaga
     5521 aataggctga acaatccagc atattccatc cactgccatc tcgaattttt catattagaa
     5581 gctccattca tccagtgatt gtttcacata ggtagctggg ataggcaacc caggtacatc
     5641 ttcttctttt gtaaaacctg ataaaacgag caaagtatct atattattgc gtatcccagc
     5701 ttggatatct gtttcatagt tatctccaac catgattact tcttcttttt gcagccctaa
     5761 tacttcaacg gctttatcca taatgatggc ttctggtttt ccgatgtaga tcggcggcgt
     5821 ttgggttgct gtctctacaa aagagatgac tgaccctgct ccaggaagaa ggccgcgttc
     5881 agtaggtata ttcttatctg ggttcgttcc aatgaaagta gcaccttttt gaatagcaag
     5941 tgttgcttta actacttttt cataggtcag ataattatct aagcctacca ctacatagtc
     6001 aggagcctct tcttcccatg taaatccggc agctaaaatc aagtcaatca atcctgcttc
     6061 tccgataaca tagacttttt tcccttttcc atctgctttc ataaaatcga ttgttgccaa
     6121 agtagctgtg tagactgtct ctggagctac atgaatgtca aactcatctg ctagacgatt
     6181 agctactgtt tctggtgact tagtcgtgtt attggtaaca aacaagaaag gtaattttct
     6241 tttttgcaat tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc cacgataaat
     6301 cgttccgtct aaatcaatta aatatccttt atagtccatt catccactac tcttcccttt
     6361 ttctgatcgt aaatgctgtt tttgtttttt ctacttgttt aggttgttct tgttttttta
     6421 ctttttcaga ggtttttgct gactctgttt ttcgattttt aataaccggt tttttgcgat
     6481 tagatgcgac tggtcctttt ttctcatcga tatacgcacg attatggttt tttttctttt
     6541 ttcggcgctg agttgtcttt tcttttttac cgccgattct ctcgatgaca aaatacgcac
     6601 aaccaaaatt gcaatattcg taaagatagt cttccaagga atcgattcgc tgatctggat
     6661 gcgcccgtcg attatccgct tggaaaaatc cttttaggcg tagttgttca taaccccaat
     6721 ctcctacgat ataatcatat cgggctagta catcactgta acgttctcca agcttttctg
     6781 cattaaatcc ttcacgataa tcggacacaa gccgataatg tctttcacct atcaaaaaat
     6841 tactgtcatc taacagtgtc actagctcgc ctttgacttt ttctgcttta ggctcctcaa
     6901 cgatttcttc caaagcagct gtcagttctt cggtgactgt tgtttcctgt tcagctgatg
     6961 attttttctt agcaatttct gttttatgct caacgttctg ctgttcattt tgttttgcag
     7021 acatgcgttc accacctatc ttttttatta taccttgttt ttatttgatt gggtagtgag
     7081 ctttcaagta atctcctacc acacttcgga taaattcagg gaacaaaaat tcattttttc
     7141 cagcaatctc aatcgttgga aaaaatggaa caaacatcaa atgatctacg gtagtaaaag
     7201 tataattttt attcagatcg attggcttgt tcttccaaaa gacttgatgg ttaatagaat
     7261 catatgtgat tccattatag accatctgcc caaaaatctt accgcgaaat cccattccct
     7321 tcataggata gttacgtaaa aagttacgat ttttttctat ttctaaaact aagcggatca
     7381 gatcatttcc acccaatgtg acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa
     7441 gctgatcctg attgacccgc ccttttggaa tttcagttaa aaacagacca ctgtttaata
     7501 ttgctgcttc cgtctttcct cgttctttca ccgcctcaaa ggctacttgg ataaacgaat
     7561 gctcattgaa gatatctaga ctcagagaaa atggcaaatc tgctacttct ttttctttta
     7621 ataattcatg cccacgttct agataactat ttatttcttg ttcatcttct gtaaaagctg
     7681 tcattgtttc ggtagggatg gctttggctg aatgcttaag gatttttttc tcttcatcga
     7741 tggttaaatg gacttcaccg acatactgcc cgtactttcc agcggctgct atttggacat
     7801 cattgattac gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa
     7861 ttgctggtat ttcttttgca atttggaaat catcctgaat cccgagatga ctcatcaaaa
     7921 ctaaaacatc cacttgtgaa cgtaaatctt cgactaattc tggcaaaata tcataaggat
     7981 tccgtatgtc ccagccattc ggattataag taagcggaaa tggagcagta aatgcaatta
     8041 gtccgatttt cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc catgccggtg
     8101 gctggagcgt atttttatcg aataagttat ccaggatgat atcaaactca gcatgatcgt
     8161 ataaatgatt caactcttct ttggaatttc ctacgccttc attgtttcct atcgtcgctg
     8221 catcataacc aacttcattc atcagcaaga tattttcctg tccattcgtt gcttctgaca
     8281 atggatgcca acgatccaca aaatcaccta gatctactgc aatcgttgtc gttttttctt
     8341 tcttttcgtt ttctcgttta cgttgatcca aaaaccttct gatccttggc caattttcta
     8401 aatgagaatg cagatcattc gtatgtaaaa tcactatttt ttccatgtac cctctcgcct
     8461 ctctttatct ttatcatacc ataggcagct ttttcttcca ttgaaacaag ctctatttca
     8521 gagtttttcc tgccttgaag caaattgtat aagtacaata ttcgataaat cagtctggct
     8581 aaaaaatcag gaacattcat ttttacagta ctataaaaat aaaaaaaaca gacccgaaag
     8641 tctgttgtcg tttcattata atcagacaca tggcggaacc tgcttagtgc tgcttccttc
     8701 cagacctgac acgcttcaca agcccaccat tgtcctagcc tttcggcaaa gaatagtata
     8761 ccttattttt cattcgttga ccagtctttt tttaatttct tttttcgctg gcggagcttt
     8821 cggaaaaagt cagtcaaaat catcccacat tcgtcttcta aaatgccacc ttctacatat
     8881 gcttggtggt taaaccgctc gtcctccaaa agattcatca aggtaccggc tgttccgcct
     8941 tttggatcat acgcgccgaa ataaacttct gctacacgag acaaaatcat cgcgccgctg
     9001 cacataggac aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc
     9061 actttttcac aggcttcctt gattgcaagc atttctgcat gtgtagtagc atcttgcgtg
     9121 gtttctctaa ggttatatcc tcttccaatc acttttccat ccaatacaac cactgctcca
     9181 atcggaactt catgaagcat ttctgctttc tttgcttctg ccaatgcaag gcgcatccat
     9241 ttttcttttt ctactgcatc aagatttgta tttaccaatt acccttccct gcctttgttt
     9301 tttcttgttt tactttagca tatgataaaa aggctgtaac taactttttt ttctatggta
     9361 aaataaatac aagaggtgaa caagatgaaa atggataaat tacttgctct ttacccacaa
     9421 gcacagaaaa aaaactctcc agcatcagaa gcggatatcc tttcttttgc aattgatgat
     9481 gcttttatct ggatcaaaca agattcatta tctcaacaag aaaccagcct tttgaaagcg
     9541 ttattccctg taatcaacga tcaaaaaaaa cacccatggt atcgctatct ttttaaagat
     9601 gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga atcaaaagga
     9661 gaattcttaa aaaaagaatg gcaagatact attttggaat tatttcgcta ttctgctgat
     9721 ttcttttttt atacagacac agatgcgctc ttagtcgaac aaataaaccg tgattatctt
     9781 gatgctactg aaatcaacgg tatttttcta tctttagatg ctgattttga tacgacaacg
     9841 tctgtattcg ttggttcttt ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa
     9901 gaaagaaaaa tttttttaac agagcaaaac aatctttata ctcacagccg tactttttct
     9961 atgcaagatg tggctttaca ttactataca aaagatactg taaaagatag taagttgatc
    10021 aaaagttaca tagaactgat caaacaaaat gaaatggaag tgatcattca agaattatgg
    10081 aatgaattag gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg taatactcta
    10141 aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa agccaacgat
    10201 ttactgtttt gctatctttt attgcttcat gaacataatt aaagatagat taaaggggtt
    10261 gtgacaataa ttttgtcaca accccttatt tcgaatgaat agtgttcaaa agctagtttc
    10321 tcttacatca ttttttcaac aaaatgcatt agccggtctt ttacatcttc ggttgaatct
    10381 ttgattgaat caccataaac agagattttc ttttttcctg atttgatttt tgctaacatt
    10441 cccatcaaag aatccaagtc actatctgaa agaccatcta cgatttctag taatttttca
    10501 tttccatcaa atttgtcata gacgaatttt tttactttcg tccgagtagt catatgatgg
    10561 atcttttcca ttacttttcc cgatacaacg actgctgtcg ctacactggc agcggctgct
    10621 acgcttaatc cgattgttac ttttgttgat gttttcataa gtatcagctc ctttttatct
    10681 atctccatca tagcatagac aaaaaaataa acgaactaat ccactcagtg aaatctcaat
    10741 cctttaggga aaaagttctt tactgttcct tttacttgtt tcccaaatcc gacaggtatc
    10801 ttatctaata ctagcaaaac ccatccttga ttcccatcac gttgaaatgt ttctcctgct
    10861 gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag gtatattctc tatctttttt
    10921 gtagcaagcg ctaatgcata gctaggttca aatcgattct ttttgaaatc tcctaaatgc
    10981 aaacctgtac gtacgacttt taaaccatcg agagaagggg ctaactcagg aacttcccaa
    11041 agatggtcgt tgaacactaa aagtcttcca gtagcttcat aatgaaaatc atttgaaaac
    11101 tccgtccaga gtttttcctg ctcttttgtc atttgcactt tacttttttt tcgtgtcttc
    11161 ttttctttat gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa atgtccttca
    11221 ccctgatctt tatgaggcca tatacgtatt gttttttcca aaccagctac gcttccccat
    11281 tcagaacgtc ctgaactaac ggattgagtc aaaggaatct cctcaatcgt tactggataa
    11341 ttttctacaa gccagctaat gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa
    11401 taaatcagtt gtcctttatt ttttagcatt tttattgctg aagacaaaat ctcttgctga
    11461 cgtttttgac agtatagcgg agattcttct gtccactctt ttattgcatt tggatctttt
    11521 cggaacattc cttctcctga acaaggagca tcaaccacga tccgatcaaa aaagcccgaa
    11581 aaatgaggga caagttctgc gggtgcatga tttgtcacta ttgcgttcga aactccccat
    11641 cgttctatat tttctgataa aatcttcgca cgtttcggaa agatctcatt cgtcactaat
    11701 aaccctttcc ccttcatttg agcagctaat tgggttgatt taccaccggg agccgcacac
    11761 aaatccaaaa ctttttcacc gggttttgct gcagcagctg ttccaacgat cattgcactt
    11821 ggttcttggc tatattcata cccagcttga tggaggaaag actttccatt gacagtgcca
    11881 agaaatccct cattgctgta gggagcgggt tgtaattctt cgctatggta agtttggacc
    11941 atgtccaatc ctgccggttt tagtggattc caccgaaatc cttttttcac gcttccttgt
    12001 tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata ttttttgata
    12061 aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta gacattttac
    12121 aatctttttt acgaaaaata aaggtggctg atctactttt tcatgttgct gcctaaggtg
    12181 actgttcctt taagatattt tccaagtatt catccgtcac ctcgttttta tcccaaacag
    12241 cgttcatcat ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt cccaatgcat
    12301 taagccatac caattcaagc gacttttctt ctttgatctt cgtgtgacct ttgacatatg
    12361 agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc agattttgca atgatttcac
    12421 gaatactttc atggatagaa aaaaaatctt tcaataagta gacaatgctt aaaacatcat
    12481 cggaactgca ttcagctaag tagttttcaa aataaacagt gatttcttct aatatcttct
    12541 cttttaaccc gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga
    12601 actctgctaa ctctcttgca gtaattgact tcattccata ttcttccacg tataccatac
    12661 ttttgaacag caaatattct ttactataat ttgttactct ccccatcatt cttacctccg
    12721 tctcttttca tctacccata aataaataac ataaataatt ctaaaaatat tattgataat
    12781 tatcaaaaca tatttagtta tatccattaa aaatcaattt ttccgcctta aaaacaagaa
    12841 tatcttgaca aaatacccta ggaaataaac aatagaataa acaaggtaac aaaaaaatta
    12901 aataacgtta tataaaaaag aaggcgacac tctgtgagaa taacacagac tgaaatgact
    12961 ttccactcac agtttttgtc taactatata caaaaacaga ataaaaagga ggtatttatg
    13021 ctaattgaag caagaaaaaa agagttactt gttctacaaa tggtcattca aaaaaataat
    13081 tgttctttac aagaattatc caatgactta catattccaa aacggacaat caaagaactg
    13141 atccgaaaaa taaatgtatc actcaagcaa tttttattga ttgatgaatt tatttattct
    13201 aatcataaag gagagatcca aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt
    13261 tctaagttaa aattatgcta cctaaaagaa tccaatcgtt ttaactattt gatgttacta
    13321 atcaattatc cacaaactgc tgtaccaaaa aaatatttac ttgaacaact ttatatatcg
    13381 ccctcctatc tcgaaaaact gacaaaacag ctcaatcatt ccttaaaaaa attccattta
    13441 cggatcctat ctgtaaaaaa ttgctgtact cttgaaggaa atgagttatc tatccgtctc
    13501 tatctttact tttttctttc agatacattt ggaggtaacg attggccatt ttcctcactt
    13561 gaattaaatg cactaaaaat gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa
    13621 gaaaaaaata tctacttgct gttaacttta ttcatattac gaaacaaaca acaggcatat
    13681 tttccaaaaa cttcttctga aatcactgaa ttgatggact tattgcagca tacacatgat
    13741 tattcagaat actttacttc tttttcagtg agtcgccctt gtgaagagac agaaacagag
    13801 agagcctact ttaattattt tttctattgt ttttttccaa atcaaatctc ttttcgacaa
    13861 aaacaggaga ttggtttttt attttccaca agcaaaaacc ttatttgtcg acaagtcggt
    13921 caattgatga cttacttaac tcaacagttt cctctcaacc atttagaaaa taaaatacca
    13981 atttattttt attttcttgt gttggcagtt gtttttttcg aactgttggg tgaatccagc
    14041 agtagttttt ctacactttg ctttccagaa gccacgtcca atcattctag ttcttttata
    14101 caggaaaaga aattaagaga aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac
    14161 caaatcagtc aataccttag tcaattgatc caacacttac tacaaacaga aacgaaagag
    14221 cagttactca tccatttgca gataaccaaa cagtttacag gaaattatca tcttcaaagc
    14281 catttgcagc agttatttcg caaagatgct gttaaaatta ccgcatattc agaacatgct
    14341 gatttgatca ttacagatca gcatgagaaa atttcagatg aaacggagac tttctatctg
    14401 gatacaacgc acaatacagt gttatttggc aagttaataa aagcggttca tgaactgtat
    14461 atgaaaaaag gttatgagaa caaaacaaag aggatgtgac aaaattcttg tcacatcctc
    14521 ttatt
//
LOCUS       DANKAZ010000064        14315 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000064 DANKAZ010000000
VERSION     DANKAZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..153)
                     /locus_tag="PWY67_001898"
     CDS             complement(<1..153)
                     /locus_tag="PWY67_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2058160.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHI"
     gene            complement(632..1351)
                     /locus_tag="PWY67_001899"
     CDS             complement(632..1351)
                     /locus_tag="PWY67_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2058161.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1400..3046)
                     /locus_tag="PWY67_001900"
     CDS             complement(1400..3046)
                     /locus_tag="PWY67_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2058162.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3225..3815
                     /locus_tag="PWY67_001901"
     CDS             3225..3815
                     /locus_tag="PWY67_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2058163.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3907..5412
                     /locus_tag="PWY67_001902"
     CDS             3907..5412
                     /locus_tag="PWY67_001902"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL2058164.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5496..6848)
                     /gene="celB"
                     /locus_tag="PWY67_001903"
     CDS             complement(5496..6848)
                     /gene="celB"
                     /locus_tag="PWY67_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2058165.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6848..7366)
                     /locus_tag="PWY67_001904"
     CDS             complement(6848..7366)
                     /locus_tag="PWY67_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058166.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7382..7708)
                     /locus_tag="PWY67_001905"
     CDS             complement(7382..7708)
                     /locus_tag="PWY67_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL2058167.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7721..9700)
                     /locus_tag="PWY67_001906"
     CDS             complement(7721..9700)
                     /locus_tag="PWY67_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2058168.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9721..10035)
                     /locus_tag="PWY67_001907"
     CDS             complement(9721..10035)
                     /locus_tag="PWY67_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL2058169.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10203..10496
                     /locus_tag="PWY67_001908"
     CDS             10203..10496
                     /locus_tag="PWY67_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL2058170.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10574..11686)
                     /locus_tag="PWY67_001909"
     CDS             complement(10574..11686)
                     /locus_tag="PWY67_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2058171.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            12073..12252
                     /locus_tag="PWY67_001910"
     CDS             12073..12252
                     /locus_tag="PWY67_001910"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058172.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12446..12616
                     /locus_tag="PWY67_001911"
     CDS             12446..12616
                     /locus_tag="PWY67_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2058173.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13040..14315)
                     /locus_tag="PWY67_001912"
                     /pseudo
     CDS             complement(13040..14315)
                     /locus_tag="PWY67_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
BASE COUNT         4408 a         2955 c         2460 g         4492 t
ORIGIN      
        1 aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa agaaaaaaat
       61 catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc atagattcat
      121 aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga ttcattcgca
      181 acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg aaaatgaaac
      241 aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga aaaaaacggg
      301 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac
      361 accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga gatcgtgtcc
      421 ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt cttgtcacag
      481 tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa gccgaaattt
      541 cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg cgctacaact
      601 actctgctta ttcttttagc tatccattca attatttttc tctcacttgt tctagctctt
      661 ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa aattttccca
      721 tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt cgcttaactt
      781 ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc tcttgatcta
      841 cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata atcagtcctt
      901 ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct agatattctt
      961 tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca ttcgttaaaa
     1021 tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct tcccgataat
     1081 cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta gcagaaatga
     1141 ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg atttctccta
     1201 aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc accacttgtc
     1261 cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg ctgccaagtc
     1321 ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa agagcatcaa
     1381 tctagctctt ctgattcact tattttattt ttaatttatg tcgtaaacgc gctattcctg
     1441 gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac actaaagccc
     1501 ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct tcgtttaaaa
     1561 aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag gtaagaataa
     1621 agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact tcatggattc
     1681 tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat aaagggccgt
     1741 atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga aacccaataa
     1801 ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg gaaactagca
     1861 tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta cttcctagag
     1921 aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca ccaaatgtcg
     1981 ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt gccagaccta
     2041 ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac gcgatcgatg
     2101 ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa atcgaaaaaa
     2161 gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc ataaaagtga
     2221 actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac ggaatcgctt
     2281 ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt tcgctgtagt
     2341 gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg acaagaatac
     2401 ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc acatagtcac
     2461 catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata cgtgccacct
     2521 gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc aaataccctc
     2581 gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc atcgttggaa
     2641 tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg tacatgaaca
     2701 aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat aatcgtcgtg
     2761 aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc gcagcaggta
     2821 ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata ttgaataatc
     2881 cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata tagacagcgc
     2941 ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt gccatttttt
     3001 cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt cagattccaa
     3061 ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca atcgatttta
     3121 tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt ggtaaagtaa
     3181 agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca caagaaataa
     3241 aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg aattggttct
     3301 tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta gttgaaacgg
     3361 ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg ctgcttctag
     3421 ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt ctgctttttc
     3481 ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc ttttcactga
     3541 tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc ggggcctcaa
     3601 atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt ccaatgccgc
     3661 aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt tttggattgt
     3721 tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg aaaatactca
     3781 tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca tagcggaaac
     3841 aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa agcgagggat
     3901 caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga aaatctatta
     3961 aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc tgaatttctt
     4021 cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg taaaggaatg
     4081 aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata ttatgtatca
     4141 gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg taaagcaatg
     4201 gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt gatcggattc
     4261 actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt ggatcacaca
     4321 acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg caaaacatat
     4381 ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat gtcagaagca
     4441 gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta caaaacacaa
     4501 cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc tgaccatatc
     4561 agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca attgatcttg
     4621 aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact tagggaaaca
     4681 gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag caattatcaa
     4741 gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga agatcgctta
     4801 gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca agaaaatgca
     4861 gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc acaagacgga
     4921 ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa tggagcgcat
     4981 gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa atttgccaaa
     5041 aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa caaagcaatc
     5101 tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc ctttttgacg
     5161 gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa tgaagcaatt
     5221 actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat tcgacgcgct
     5281 ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt ggatccctat
     5341 caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt gaagcggttg
     5401 attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa aaaagggact
     5461 gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc actagctaat
     5521 tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt gataaatggg
     5581 aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc tttccaatca
     5641 ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat ctgtgcaagc
     5701 atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga agccatcgga
     5761 gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc gaaaatgatt
     5821 ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac tttcagctga
     5881 tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc tgatccacca
     5941 acggttacat aagaattgtt gaattctcct gccaacggat aatttgcgcc ttgtgcgtta
     6001 gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt cgctccatgg
     6061 atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc aagccatgta
     6121 ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat atccgttcct
     6181 agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa tgttgggatc
     6241 agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt tatgacccaa
     6301 tttctcttga cacacataca atacaattcg accgcaataa tcgacataat aatagctgta
     6361 aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc atttactaca
     6421 gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat gatcaattca
     6481 gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg attaacattt
     6541 aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat ggcaaagtaa
     6601 atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact aactttgaaa
     6661 aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat atttaaaacc
     6721 aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc catgacagcc
     6781 cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt ctgcaaaaaa
     6841 tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt ttaatacgga
     6901 tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata aatgcactgg
     6961 caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg ttatttgcca
     7021 taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact aataaattaa
     7081 agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg tgttttcgat
     7141 aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc atcggtaaac
     7201 tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc atgaaaaaga
     7261 aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt ttcaaactaa
     7321 gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg atccttcctt
     7381 tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat aatgtcatcg
     7441 tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt tttgtcccgc
     7501 tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc tcttcatttg
     7561 cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag cgaaatgctt
     7621 catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa gtcacttgta
     7681 gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc agtttgataa
     7741 atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt acttttagct
     7801 ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt tcgacataag
     7861 aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc atcccagaag
     7921 gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga tgtgggactg
     7981 ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc tgctttcgca
     8041 tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa tcatgaagaa
     8101 tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac aactgttcgg
     8161 tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct gaggaatgat
     8221 agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca ttcaaaaaaa
     8281 tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata gaagaaataa
     8341 tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt acacttacca
     8401 ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg gcactgccgt
     8461 aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct ttttccaatg
     8521 ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta tagtttttca
     8581 aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga ttttctttct
     8641 ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga atcatcatcg
     8701 gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat gaaaaatgaa
     8761 attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc tgatcatttt
     8821 gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca atttcttctt
     8881 cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgctgc gccacttgat
     8941 actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca aacccttctc
     9001 ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata aaatccgaaa
     9061 cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct tcaaaactga
     9121 tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca taatggtttc
     9181 caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg atccatgtcc
     9241 cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc ttgatttctt
     9301 gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa tcatccataa
     9361 aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga tcttcggact
     9421 cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga ctcaaaaaca
     9481 agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt ttagcaatga
     9541 tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta cggattgtcc
     9601 gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc ttcttgtgat
     9661 caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac tcctattcca
     9721 tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga ttgggacata
     9781 cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt ttttgaattt
     9841 atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac tattcttaat
     9901 catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc ttttttgctt
     9961 aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg cacaaataat
    10021 caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc atatcttgta
    10081 agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt gtatcatatc
    10141 ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca ggaggggatt
    10201 tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc agaaaatttg
    10261 cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa ggaaacgaga
    10321 aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc gaccgttgga
    10381 aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa ataaacgaac
    10441 tccgtaaaaa gtataaatta aaaatgaaag tcgaacgttt cactgattta ccgtaaaaag
    10501 atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt ccttattttt
    10561 tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg ataagtattt
    10621 cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca aaaatgcgga
    10681 tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca ccaaaagaat
    10741 taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca cctaatatac
    10801 cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat ccaatcaatg
    10861 agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat ccatagctag
    10921 atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg acatactctc
    10981 ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt ttttcctgcg
    11041 aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc ttttctcgat
    11101 gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag ctacttgttt
    11161 gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata aagagatatg
    11221 aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc attaatatgc
    11281 tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc agctggacat
    11341 acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa tgggaaatct
    11401 tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc acagcgtatg
    11461 gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg gcaaacaata
    11521 cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct tttttagaag
    11581 ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac tgataaaaat
    11641 ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat gacaggttgt
    11701 aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt tcctattcat
    11761 gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa ataaacaact
    11821 gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc aatagacaaa
    11881 aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac attgatcgtt
    11941 gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc tgtggtagat
    12001 ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt ccactttgaa
    12061 taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac ctgtaaaaac
    12121 tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc gatttcaaat
    12181 catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt ttttatttcg
    12241 aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt gagaagctat
    12301 ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa tttcgttctc
    12361 acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca gaaaaattaa
    12421 tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc ttagctgcac
    12481 taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact ttcagctcac
    12541 caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt tttccacgcc
    12601 tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga caaaagaccc
    12661 gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa ttacaactga
    12721 tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga gtcattaggc
    12781 aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt ttttattaac
    12841 tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt gtctttactt
    12901 atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg tatacccctt
    12961 ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa actatgctta
    13021 ttcaattttt tattttagct catatatttc gtttctttct aaattttata ctgatgaaaa
    13081 caagtagcac acccaatatt gacaatataa actggctaat gatggtttct ccagtttttg
    13141 gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag ctatcagcaa
    13201 cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg atcttgaaag
    13261 ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc agctgatatg
    13321 ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt agacctgact
    13381 gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg acttctccgc
    13441 tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcgattt tctttcgtca
    13501 attctaccac cgctgtctga cttctttcga tctcaaactc gattggtgtc gcatccaatt
    13561 cataccccgt tggcgcttgg gtttctacca gctgatacac tcctggtgct aagccgtcaa
    13621 tcgccagttt tccatctgct cctgtggtta gacctgactg cagaacggtt ccattggcat
    13681 cttgtaactc aaaaacagct ccttgcagga cttccccgct ttggtcatcg atctttgtca
    13741 acacgacacc gcctggtgta agtcggtttt ctttcgtcaa ttctaccacc gctgtctgac
    13801 ttctttcgat ctcaaactcg atcggtgtcg catctaattc ataccccgtt ggcgcttgag
    13861 tttctaccag ctgatatgct cctggtgcta aaccgtcaat tgccagtttt ccatcttctc
    13921 ctgtagttag accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc
    13981 cttgcaggat ttccccgctt tggtcatcga tctttgtcaa cacgacaccg cctggtgtaa
    14041 gtcggttttc tttcgtcaat tctaccaccg ctgtctgact tctttcgatc tcaaactcga
    14101 ttggtgtcgc atctaattca taccctattg gcgcttgagt ttctaccagc tgatatgctc
    14161 ctggtgctaa accgtcaatt gccagttttc catcttctcc tgtagttaga ccggtttgta
    14221 gcgtttctcc ttctcggttc tgtaactcaa aaacagctcc tgcaggattt ccccgctttg
    14281 gtcatcgatc tttgtcaaca cgacaccgcc tggtg
//
LOCUS       DANKAZ010000065        13810 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000065 DANKAZ010000000
VERSION     DANKAZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(221..1423)
                     /locus_tag="PWY67_001913"
     CDS             complement(221..1423)
                     /locus_tag="PWY67_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2058174.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="PWY67_001914"
     CDS             complement(1426..1620)
                     /locus_tag="PWY67_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2058175.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="PWY67_001915"
     CDS             complement(1667..2515)
                     /locus_tag="PWY67_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HDL2058176.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="PWY67_001916"
     CDS             complement(2720..3070)
                     /locus_tag="PWY67_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058177.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="PWY67_001917"
     CDS             3665..4111
                     /locus_tag="PWY67_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2058178.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="PWY67_001918"
     CDS             4735..5727
                     /locus_tag="PWY67_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2058179.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="PWY67_001919"
     CDS             5743..6627
                     /locus_tag="PWY67_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2058180.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="PWY67_001920"
     CDS             6676..8283
                     /locus_tag="PWY67_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2058181.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="PWY67_001921"
     CDS             8284..9768
                     /locus_tag="PWY67_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL2058182.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="PWY67_001922"
     CDS             9945..10937
                     /locus_tag="PWY67_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2058183.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="PWY67_001923"
     CDS             10989..11858
                     /locus_tag="PWY67_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2058184.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="PWY67_001924"
     CDS             complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="PWY67_001924"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2058185.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DANKAZ010000066        13429 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000066 DANKAZ010000000
VERSION     DANKAZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..914)
                     /locus_tag="PWY67_001925"
     CDS             complement(<1..914)
                     /locus_tag="PWY67_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL2058186.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="PWY67_001926"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="PWY67_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL2058187.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="PWY67_001927"
     CDS             2006..2176
                     /locus_tag="PWY67_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058188.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="PWY67_001928"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="PWY67_001928"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL2058189.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="PWY67_001929"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="PWY67_001929"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL2058190.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="PWY67_001930"
     CDS             4440..5321
                     /locus_tag="PWY67_001930"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL2058191.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="PWY67_001931"
     CDS             5318..6034
                     /locus_tag="PWY67_001931"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL2058192.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="PWY67_001932"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="PWY67_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL2058193.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="PWY67_001933"
     CDS             6751..7752
                     /locus_tag="PWY67_001933"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL2058194.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="PWY67_001934"
     CDS             7749..9110
                     /locus_tag="PWY67_001934"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL2058195.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="PWY67_001935"
     CDS             9070..10221
                     /locus_tag="PWY67_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL2058196.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="PWY67_001936"
     CDS             10196..11365
                     /locus_tag="PWY67_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2058197.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="PWY67_001937"
     CDS             11540..12919
                     /locus_tag="PWY67_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2058198.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="PWY67_001938"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="PWY67_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DANKAZ010000067        13343 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000067 DANKAZ010000000
VERSION     DANKAZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            14..160
                     /locus_tag="PWY67_001939"
     CDS             14..160
                     /locus_tag="PWY67_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL2058199.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
     gene            complement(222..2033)
                     /locus_tag="PWY67_001940"
     CDS             complement(222..2033)
                     /locus_tag="PWY67_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL2058200.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2030..2395)
                     /locus_tag="PWY67_001941"
     CDS             complement(2030..2395)
                     /locus_tag="PWY67_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL2058201.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2558..2953)
                     /locus_tag="PWY67_001942"
     CDS             complement(2558..2953)
                     /locus_tag="PWY67_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2058202.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2971..3933)
                     /locus_tag="PWY67_001943"
     CDS             complement(2971..3933)
                     /locus_tag="PWY67_001943"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL2058203.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3933..4145)
                     /locus_tag="PWY67_001944"
     CDS             complement(3933..4145)
                     /locus_tag="PWY67_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL2058204.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4166..4864)
                     /locus_tag="PWY67_001945"
     CDS             complement(4166..4864)
                     /locus_tag="PWY67_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL2058205.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4884..5426)
                     /locus_tag="PWY67_001946"
     CDS             complement(4884..5426)
                     /locus_tag="PWY67_001946"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL2058206.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5558..6562)
                     /locus_tag="PWY67_001947"
     CDS             complement(5558..6562)
                     /locus_tag="PWY67_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2058207.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6559..7548)
                     /locus_tag="PWY67_001948"
     CDS             complement(6559..7548)
                     /locus_tag="PWY67_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2058208.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7545..8351)
                     /locus_tag="PWY67_001949"
     CDS             complement(7545..8351)
                     /locus_tag="PWY67_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2058209.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8517..9473
                     /locus_tag="PWY67_001950"
     CDS             8517..9473
                     /locus_tag="PWY67_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2058210.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9550..10068)
                     /locus_tag="PWY67_001951"
     CDS             complement(9550..10068)
                     /locus_tag="PWY67_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2058211.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10156..10305)
                     /gene="rpmG"
                     /locus_tag="PWY67_001952"
     CDS             complement(10156..10305)
                     /gene="rpmG"
                     /locus_tag="PWY67_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2058212.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10533..10979
                     /locus_tag="PWY67_001953"
     CDS             10533..10979
                     /locus_tag="PWY67_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2058213.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11174..13069)
                     /gene="asnB"
                     /locus_tag="PWY67_001954"
     CDS             complement(11174..13069)
                     /gene="asnB"
                     /locus_tag="PWY67_001954"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL2058214.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4151 a         2832 c         2276 g         4084 t
ORIGIN      
        1 atttgtatct ggaatattag taggaagtct ggcaacagcc gctgccgttg ctggtttggt
       61 tgcttctgtg aaaaaaacag tgatcgatcc aattgacgaa aaagaagcaa tgatcgaaga
      121 aaaccgtaaa aaagcaatgc gtaaacgcgt ttctcgttaa taattattta aaaatacgca
      181 tgaagacttt ctaaaaaaca gataagtcat catgcgtatt tttatttgtc agtcaatata
      241 agttgtgcta ttttatctgc ctgtctcaga ttccatggat ctgtataagg tctagctact
      301 gctgcagtta gccaatccac accttcaaca ggtattccat agcaataaaa gtcagactgg
      361 atttcccctg aagcatcaat cacgcgattc gtacctcgct caacatctat agcttctgaa
      421 tgataacttc ctgtattatc tgaaaatgaa aaagggcgaa caagtttgtt ttccttcaaa
      481 ttttttatga caggattact tgaacgagaa aagcttgttt gtggcaatcg agcttcaatc
      541 aaatagcgac tgctgatttc tttatcttgt agagaaataa catagcggtt gtgcttttgc
      601 tgaatatcaa atggcggttc gatcaaatga aaaatacccg catctatcag tgctgctaat
      661 tctttcgtcc gaatgattgg cggaccgatc gttaaaaagc tatttagatg agtaaaccat
      721 ccccatagta tttctttgta ttccttagca gaaaaaacgt gccattcaat agcctgccgg
      781 attggatcac gccagtcttt taatgcatca aatgttgaag caaatgctcc tgtgcaattg
      841 cctttttccg cttcttttat ctgcccttca ataaatattc ggctagcttc tgaaaagggc
      901 tcattctgat aggcaagagg agtttctaaa tacgaccaat cccatctata agggattagt
      961 gctggatgct ttccgagaca ctcctctgaa gagagggaga gaaaatcatg ctcaaaattt
     1021 ttctttggaa tcggcaagtg atattcttcg atgattttct tgtaataaaa caactcaacg
     1081 tcttttttca gatagtcaaa aaatgtttcg ccagtcaaaa atcccgactg atgccattgc
     1141 tccaggtttt cttttgttag taaccgtggc catgccattg ctccgccttg cttttgattt
     1201 cttcctcgag ggtaataagg gagccccctt cttgatccac tgtatacaat cggttccttg
     1261 cctgatggat gatagaccag ttttcccttt tcgttctcga agcgccctcc tcttgatact
     1321 gtaaacaatc cgacataatc aaaaaaactt aagcctagac ctcttaaaaa gacattttct
     1381 ttaggcggta tcttcgaaag atctacatct gcaggattag aaggaagctg atacagtaaa
     1441 acatttttct ttgcgtagtc tacccacact ctttcttctt ctgtctcatc attttcccag
     1501 tgaccgcttg ctaaaacaag ctgatcgata gaatagcttt gctgatctgt ctgtaaaaga
     1561 taaccatctt ttcttttgct tacttttttt actaaagaat aaacaaactg aatagaagct
     1621 ggaaattctt cttgcaactt tgagaaaaac cagcgttgat agagtccgta taaacatcga
     1681 gtagattgct gatctttttc cagacgattg atttcttcta taattagttg cttatttttg
     1741 gttaccgtct gttcgatata atctgtagca ggtcctttac tccattgaaa caagttgggt
     1801 ccctgtgtta ccaatcctcc actggacaat gtttcatctg taaaaagcgt cacttgttga
     1861 gaaacagaat tcattaataa ctccggtggc tgattcaacc gccaaactcg acctccagga
     1921 ccattgggat cgaaaagaaa aagttcaaga gaatgtctgt cctgattatt atggagaatc
     1981 ctttctgcta cacttaatcc tctaggtccg gcaccaatga tccctatttt catttgcatg
     2041 cctccatttc gataaatttt ttcaataaaa cagcatggtt ctgatgttca ttttttgcac
     2101 cataaacaaa agtaatgact ggatgttctt tcataagttg actgagttta aaaatagctt
     2161 gcttcttttc ttcttcgaaa atcaattctt gctggtattt ctttgcaaat gaagcaaatc
     2221 gttctggtac atggtcaaaa gttttgcgtg cttcattact aggtgcaatt tctttcaacc
     2281 acagatctaa ctgcgctttt tcttttgaaa ttcctcgtgg ccagaatcta tccacgagta
     2341 ttctatagcc atcctctttt tcagcatcaa ggtaaacacg ttttaatttg atcatagttg
     2401 tttccttctt tctacctttt cgcttagctt atatatgtag ataatagcac aaaataaaaa
     2461 aaatgaacgg cacaactatc cattgcgccg ttcatgtgat cttggtcaaa atctaatgat
     2521 tgattcggtt tttaaagctg tcatacaatc atctgtttta tactttttta cttttgattt
     2581 ttccagacca tccgtcatat ccacccttaa gtaaataaat ctcttgataa cctgctttac
     2641 gtaatttatt tgctgtacgg atgctcaaac ttttcttttg atcgtaaata tagacaggct
     2701 ggtcttttcg aatagatcct aatgaatctt tcaaaacagt gtaagggatg tttcttgcac
     2761 ctaaaatatg cccagcatca aacacatctt tttcgcgaac atcgattact tgtgcttttc
     2821 tcattccttc tcgaaattct tcttctgtca aagtcgttgc tgaacgtttg gccattactc
     2881 gtagatagac ttcccaaagg atgattgcaa gcacaatcaa tagcaaaatg atattaatta
     2941 cccataaaac catgtaatgc ctccgttttt ttaagcaaat ttcaaggcta atgaggcagc
     3001 accaatgaca ccagcttcat taccaagctc tgctaatttg atttttgttg attctcttac
     3061 ttgcgggaaa gtaaattctt taaaatattt ctctacacgg ctacgtaaaa attctcctgc
     3121 tgccgatact cctccaccta acacgatact tgacggattt aatgtattcc ccaagtttcc
     3181 gcaagctaaa cctaagtaga agcatacgcg atcaacaacc atcaatgcaa acggatcgtc
     3241 ttcttgagcc atttcaaaga tatctttact tgttacttct tctccgttgt ctagcattgc
     3301 tttcagtttt gaatcccctg cgtattcttc tgccaagaaa cgacctaggc gtaccacacc
     3361 tgtagcgcta gatacagttt ctaaacatcc gcgtttaccg caagtacatt cgaacccttc
     3421 aggatccaca gtgatatgtc cgatttctcc tccagctccg ctcacgccgt gaatcaaatt
     3481 tccgccagca acgatacctc cgccaacacc tgttcctaga gtaacgaata caacgtctgg
     3541 atcgttttct ccagcacctt tccatcgttc acctaaagca gcaacattcg cgtcattatc
     3601 gatagcgaat ttgattcctg ttcctgcctc gatcagtttt ttcacaaatt gaacttctgt
     3661 ccagtttaag ttatacgcac caataaccgt tccagcttcg ctgtccacgc ttcctggggt
     3721 acccatgccg attccgataa agtcttcggc tttcatatta tataaattca aacgatgatt
     3781 gattgattca atgatttcag gaacgatatg cttaccatca tccaaaatat tggtatcgat
     3841 gctccatttt tgctgaatct ctccttctgg tgttaaaatt gcaaattttg ctgttgttcc
     3901 acctaaatca atcccaataa tcttcttgtc cattacaaat ttcctttctt tcggctttct
     3961 tcaattcggt gctctctttt taatatgcgc cacgctgtca tgtatgtatc atgatcaatc
     4021 aaacggctat catacagatt acgcaattcg atagtcatta attcaatgtc atagatgcgt
     4081 gcacccacat agataaagat gccaaatttt ttgaacagct gctgtacatc atataagctc
     4141 tccatcttta agcacttcct tcatattata caagtaaacc atattttttt aaacccagta
     4201 aaatacatat tacaaaaaga aatacaaaaa ctagtgtaga aagaatcctt tggtgtatcg
     4261 aatagcttgt ctgttgtttc ggtagaccca aaacattccc taacactaat ccgccaatta
     4321 gcccaccgac atgtccccaa acatctacag aggtatccag aagaccaaaa acaaatgtca
     4381 acacaataaa taaaagaaaa tgtctgacta gctgctggat cgctgggtta tgtttaaaat
     4441 gaaaccctaa gataaacaac gcaccgaaca aaccgaatat cgaagtactt gcaccaccag
     4501 acagaacatt cgcttcgtta aaagcaaagc ttgctgtatt tcccattatc ccgctgaaca
     4561 tataaatcaa gaaaaaacgc caatggccat agattgcctc gatttgctgc cccataaaat
     4621 acagtatcag tgagttcatt acgaaatgca tcagtccaaa atggatgaag attggtgtca
     4681 ctaaacgcca ccattcattg aagtgaacaa gataaggacc atacattccg gcataataag
     4741 ggatctcaag tcgcggaaat aattccatca tgaggaagac cgctgtctgt atccctaaaa
     4801 aagcataggt ccataacggt cgtcttaacc atgcacgcaa ttttatttgc tgttgataat
     4861 tcatgaaaac ctcccatttc attttagtca ataaacaatt gttggatctt ctgatcaaag
     4921 ctctccggtt gccattgttc attcaattgt tcacgaaaaa caaggctaca tgttttttta
     4981 tgaaaattat ttaaataccg gtcgtaataa ccaccgccaa aaccgattcg ataaccggct
     5041 ctattgtaga taatacctgg tacaatgagg agatcgatca tctccggcaa atagagcttc
     5101 tctgattgtg gctcaagaac accaaaactc gaacgtacaa gaacggactg ttcatccatt
     5161 tcataaaacg ccatctgacg tttcggcaaa gttttaggta caactatttt cttttgttct
     5221 ttttctgctc gttggataac cggctgtgta tccagttcaa attcattcga taaagtcgtc
     5281 cctatcactt ttgctgactt ccagtactcg ctggcgaata gctgtttata gattcgctgt
     5341 tctttttcct ttttcttgcc acttttggcc agatttttca gcttttcgat tcctttccgg
     5401 cgtaatctta ctttcaattg gtccaaaaac tcacccttcc ttctctcata aaatggtata
     5461 ggaaaaaaag aaaaatagca atcgaacttt caaataaatg cataaataga ataaaaagaa
     5521 gacctagaaa tgattggcct tcctttttta atgaatttca actgttcagc gcgtgttttt
     5581 gtctgataaa aagtgcatac agaaaatatg gcgcaccaaa aagggcaatc aaaattcctg
     5641 taggtacttc cccacttggt agaatcactc tagcaaccat atctgcacta agcagaaaaa
     5701 tggaaccgaa caaaccgctc agaacaaagg tccagttatt ttctttacgt acgactagct
     5761 tcgcagcatg aggagctagt agtcctacaa aagataaact acctgtcacc gctacactac
     5821 cgcttgccaa agcaacagct aacaacaaaa ggattttttg acttttagcc acgttaaggc
     5881 ctaatccttg cgcgcgttct tgaccaaatg atagcaatac aatcctctgt gcataaagtc
     5941 ctcctagcgg aattaagata accgcccatg gaagcaatgc ccaaacataa ggccagctcg
     6001 caccccagat tgatcccgct aaccatgcat tcacaaatcc ataattttcc ttagataatt
     6061 ggatcgtgca tagcaatgtt aatgcagaaa ggccggcatt aacggcaata ccggacaata
     6121 agattcgatt catacccaaa aagccatttc gatcataggc cgcaagatag acacagcaag
     6181 cagctaaaac accacctaga aaggctacaa ctagcaatag aaacggcagc gaatagtgcg
     6241 aaaagaaacc taaataaagc atcactaaaa atcctgctcc ggcattaatg cccaatatac
     6301 tagaatctgc caattcattt cttgttactc cttgcaagat ataaccagaa acgccaagac
     6361 aagctcctgc taataaactg atcagtaatc gaggaactcg aaattctcta acaatcagcg
     6421 ccaaagaaga tgattctttc cctatgaacg tggcaaaaag ctgatccagg taaataaaag
     6481 gcgtaccaat cattaaacta cctatagaca ggatgacaag tacaaaaagc aagaaataga
     6541 acgtccaata tatttttttc accgttgttc cctccttact tgtatcagta aaaacggaac
     6601 gccaatcaat gcaatgacta atccaaaagg tgtttcaaaa gggggattga tcatcctcgc
     6661 tgtaaaatcc gccaacataa ccaatccacc gccaactaaa aaagagacag gtagaacata
     6721 acgatagtcc gttccaacca ttccgcgaac aactggcgga accaacagcc caataaatga
     6781 aacaggtccg actaacgaaa cggcaatggc tgctaacact gcaactagac agaaaacaat
     6841 caaccgcgtt cgtttaggat gacttccaag agctaatgca tgtgtttcac tcaaatgaat
     6901 caaagatatt gcttttccta atgaaaaaga aaccaaaaca gcaagtatat ataaaggaaa
     6961 caatactgct aattgtttcc atgtgatatt ggcagctccg cctacaaacc aaaaagcaag
     7021 atcctgctgc agattaaaga attgggtaag tgcttggctg atagcagtaa aaaaagaact
     7081 aatagctacg ccagctaaaa tcagctgtaa aggattcatg cccattttcg aaaactgcaa
     7141 aaaaccaaat acgagcaaag ttacagcaaa agaacctgcc aatgacatta gaaacacaga
     7201 aaaatcagaa gctttaggcc aaaaagcaaa actaagggcc attgccaacg aagcacccgc
     7261 gttgattcct agtaagcctg aatctgctaa aggatttctt gtcacccctt gcatcaatgc
     7321 accgctcaat gcaaaacaac tccctacaag aaatgctccc gccacacgag gcaaacgaat
     7381 atttcgtatg atttggtgac tttgttttga tctatcaaaa tcaaacaacg cctgccaaaa
     7441 atctgctttt tccacagtcg ctgcgccatt tgaaagagca acaaaaaaaa taatcaaaag
     7501 aacaaaacag accaaaagca ggagtctatt tttcttcata gtcctcataa ttgatcctcc
     7561 tgctcttttg gcaacaaatc ataggttaga aaaactggtt tatttgttct tggaagcgtg
     7621 acaatcgttg catcaatctc aaatattttt ttcatacact cttttgtgat cacttcttca
     7681 ggtgtacctt ctgtgatcaa cttaccgttt ttcattgcta ataaataatc tgaaaaacgt
     7741 gaagcatgat taatgtcatg aatcgacatc aagatcgttg tttgtttcat ttgattgatt
     7801 tcagtcaata actgtaggat ctctaattgg tgtgccggat ctaaaaatgt tgtcggttca
     7861 tccaagatca agatatctgt ttcctgagca agcgccatag cgatccagac acgctgggtt
     7921 tgtccaccag acagcgttgc gatatcccgc atacgaaatc cagctagacc tgtagcatcc
     7981 aacgcccact caattgcttc atgatcttgc gtgtccatgc cactgaacag tttttgataa
     8041 gggtatcgtc cgtaagatac tacttcttct actgtcatac ctgtagggtg ttcactagtt
     8101 tgtgctaata atgcaatttt ttttgcaagt tcttttgaac tgtatgtaga gatatcagag
     8161 ttattgacaa tgatctttcc tgatttcaca ggtagtatac gggtaaaact tttcaataaa
     8221 gtagattttc cacttccatt tggcccaatc aaactagtga ttttcccttc aggaatcgta
     8281 acagacatgt tttcaatgat ttttttgttt tcgtagccgg ctgcaatgtc agctgctctt
     8341 aatctcgtca tttcacccat cctttcgctg aaaatcattc tcaattatga cgctgtttca
     8401 acatttcgtc aagcgctact ttcatatcta tagccaaatg aagcggttga cagcgttcga
     8461 aatatcagga ataataacca atgagaaaga ttctcaatta tgttaggagg aaagaaatga
     8521 aattaaaaac aaaagtattc acattatcta gcatattgct gctggctgga gtactgagcg
     8581 cttgctcccc ctcttcagat aaagaaacag ctacaactgc caattcgacg acttcatcca
     8641 atagtactca tacagtcaca gatacacttg gacataaagt agagattcca aacgcgccca
     8701 aacggatcat cggcagttac ttagaagact atctgattgc actaggggaa aaacctatcg
     8761 cccagtggac tgttggcagt ggttccattc aagattatct gcaagatgat ttaaaggatg
     8821 tcccaacgat ttcttatgac cttccttatg agaaagtgtt aagctttgag cctgatttgc
     8881 tgttgatttc ttcttccgct actgtcgaag gcgggaaata cgaacaatat agcaaaatcg
     8941 cgccaactta tgttgtaaaa aacggaacag atgtcacatg ggaagaacaa ctaaaagatg
     9001 tcggcaaagc tttggcaaaa gaaaaagagg cagaaaaaat catttccgat tatcaaaaat
     9061 ccatcaagaa aactcgagaa gaactagcag ataaaatcga gaacaagaca gctgcagtgt
     9121 tgtgggtgac caacaactca gcctttatgg tcagcaaaaa tcggtcaagt ggaagaatcg
     9181 tatacgacga tctggaattt ggtgttcctg atctagtgga agaggtctcc aaaaaagcca
     9241 cttctgactg gtctgctgtt tcctctgaaa agctagctga attagatgct gactacatca
     9301 tccttgtgaa cagtgacaaa gatgctgcca tgttcaatga accaagctgg aagaatctaa
     9361 aagcagtgac agacaatcat gtactgcaat tcggacctga atctagttgg ctatataacg
     9421 gacctattgc cagtcaggaa atggtcaatg atatcaaaga aaaactaaat tgattttaac
     9481 taaaaaaagc atcttcacga ttagtcgtga gatgcttttt ttcttattaa caaactagac
     9541 gcatagattt tattcttctt ttgaagcaac cctatttttt actctttccc atttttgaaa
     9601 acgatgggca tatagatttt cttgatccgt tatcccctca tactcctcta taagggcgaa
     9661 gtctgaaaaa tcaatattcc caatatacgt atcgccttca aacgtgctat cgatcaatgt
     9721 tcgccagatc cgaccagtgt aaggaagaag ttcttgaaaa atccgactac cacccgaaac
     9781 atagatatct tctgagatcg tttgactata cgacaaaagt tcatcgatcg aatgcattac
     9841 ttccgcattt tctttcgtgc gaaaatttgg ttgcgttgtc aacacgatga tatggcgata
     9901 aggaagtgaa agatccccca ttccttcata ggtctttctt cccatcacta aaattcgatc
     9961 cattgtatgt tcgcgaaaga aacgcatatc atttggcaat cgccatggta acaaaccatt
    10021 tttgccgatc agaccatttt tatcttgtgc ccacatggaa ataaacatat tatccgctcc
    10081 ttatgatttt agatgttttg agataaaaaa agtctcaatt ctttgaccag caaggaattg
    10141 agaactttaa aaacattatt ttgtttcacg gtgcaaagta actttgcgtt cgcgtgggca
    10201 atatttttgt ttttccaaac gatcaggatt gttacgtttg tttttgcttg ttagatagtt
    10261 acgttcttta caagaagtac actctaaagt gatgtttacg cgcatgtttt tccctcccat
    10321 ttttacaaga atcctctaag atgactctca gacttgatta tcttaccatg attttttcta
    10381 gattgctagt gtttttcacg attttgttaa gtaaaactac ttgacgataa atacaacggt
    10441 tttacttctc aaacaaaatc tttttctttt tgtataaata cccttacact tgtttatata
    10501 gattatgttt tttaataaag ggggatacta atatgccatg gaacatgaag gactttcctg
    10561 catcaatgaa aaacttagat aaattgacaa gaaaaaaagc aatcgacatc gcaaatgcat
    10621 tattagatga agggtaccct gataaccgtg cgatcccaat tgccattgat caagcaaaaa
    10681 aatggtatga agatgctagt gaatcagaaa aacgaacttt cgaaaaagaa aaaaatccaa
    10741 gcaagacgga tgaacacgat acgaatcctc gtgctggaaa actgcttgat tcagatgtga
    10801 tcgtagaata tgaggaagaa caatggatcg tgaaatctaa aggggcaaaa aaggcaagca
    10861 accattttga tacaaaaaaa gaagcaatcg aaaaaggaaa gcaagtagca caaaacaaag
    10921 aatcaacttt gttcatttat aaaaaagatg ggacaaaaga aaaagaaatc gattattgaa
    10981 catatgtaaa ggggttgtga cgaaaatcgt gtcacaaccc ccttatgttc gaaacaatgg
    11041 tgttcctctg ccaactccga accataagtc ataatatcta taaaacggga agagctgttt
    11101 tttgaaattg tttcttatca ctcagtgtta aacggtagga gaaaacctct tttttattga
    11161 tatttgattt tcatcaaaca gtcacatagt cgattgcttc tgcttgcgga atcgtttcat
    11221 ctacaaagta gactttatac catgtcaaga aactgaagat tgtccagatt tttctttgct
    11281 cttctttacg tccttcgtga tggtcatcta gcaacttcat gattttttct tgatcaaaaa
    11341 attcttttgc aaaatctgct tcgaataatg ctttgacttg ttcgtagccg tgttcttctt
    11401 tcaaccaagc tttgattggc acagggaatc ctaatttcac acgattcgac cattcttccg
    11461 gaagatgttt gttcgcagct tgtctgaaaa tatcttttgt gttgttttga ttcaataaaa
    11521 acttacttgg tatttgttga gccaatttca tgacttcgat atccaataaa ggtactcgga
    11581 cttccaaaga acttgccata gaaagtttat ctgcctttaa caagatatct tttggcatcc
    11641 attgatgaag atcgacatat tgcatcttat tgacttcatc tttgattcct tctgttttct
    11701 cgtaatgaac agtcatgatt tcatccacag atggtgctgt ttgatatttt ggtgttaaca
    11761 cttctgatgc ttctgattct tcgaacactc gagcatgacc gataaaatat tcttttgcag
    11821 gagcaagtga ttcatataaa tggatccgtc catggaagtt tttcattttt ccgattttac
    11881 gtcctaaatt atagcgtgtt ccttttggta attttttcaa tccttgagca atcacacgga
    11941 tgaattttga attcgtatgg aatccatacg tttggtatcc cgcaaacagt tcatccgccc
    12001 cttctccaga ttggactacc cgcacacttt gtgaagcaag atttgctaaa aagtacaaag
    12061 gtacgcaaga aggatttgaa tcaggttcat ctaagtgata ctggatcaaa gggaatgctt
    12121 tgaacgacat gtctccgtcg atcactgctg ctgtattatc taagtccaat agttcggtta
    12181 gttttctggc ttcaatcgct tcattatagg ttttatcatc aaacccgatt gagaaagaat
    12241 gatctggttt caatacagag gttacataac tggaatcaac cccgctggat aagaaagaac
    12301 caacttctac atcactgacc gtatgtgctt caatagaagc ttgaacggtt tcgtcgattt
    12361 tttcgatcca ttcttgtcgg gaatgcgttt cttttgtttc aaaatctgca tcccaatatt
    12421 gctgaatgtc tatttttcca tctttatacg tataataatg gccttcaggc aaacgataga
    12481 catctttgaa gaatgtttcg ccgtttaatg gagaatattg gaaagtcata taaggtttca
    12541 acgcttcttt gttcaattct ttgttaaaat caggatgagg cagaaaactt ttgatttctg
    12601 aaccaaacat aaatgttccg ttcatttctg cataataata tggtttgatc ccaaagtggt
    12661 ctcttgcacc gaataactca ttcttttcat tatcccaaat agcaaaagca aacattccgc
    12721 ggatcttttg caataattct gttccccatt cttcgtaacc atgcaaaagg acttctgtat
    12781 ctgcatgtgt ttgaaaaacg tggccagctg caatcaattc ttctctcaaa ggctggtaat
    12841 tatagatttc gccattaaaa ataatgatct ttgtccgatc ttcattgtaa ataggctgag
    12901 taccgccctc taagtcaata atacttaatc ttctgaatcc taaagcaaca tgtttatcga
    12961 tatattctcc tgaactatta ggtccacggt gaacgattcg atccatcatt gtattaataa
    13021 ttgttttttt gttgtccttg tcgttaacaa aacctacaat accgcacatt taactttcac
    13081 atcctaatat aaaattactg tttatttcat tcatatgcat cttatcatag aataaatagc
    13141 ggacgaatat tttctctctt acaatttcgt cacgtgaaaa caaaagatga aagtttctgt
    13201 ttttacagaa aataaagtcc caatatctaa tttaccatgt tttctttttt gaggctacct
    13261 tattctcttc gtgttaaaaa aatattagtg gatgctattt ccaaaaaaac cagcaaaaaa
    13321 agacacgacc taaagatcgt gtc
//
LOCUS       DANKAZ010000068        13312 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000068 DANKAZ010000000
VERSION     DANKAZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(44..1297)
                     /locus_tag="PWY67_001955"
     CDS             complement(44..1297)
                     /locus_tag="PWY67_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL2058215.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILIVKLS"
     gene            complement(1294..3483)
                     /locus_tag="PWY67_001956"
     CDS             complement(1294..3483)
                     /locus_tag="PWY67_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2058216.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(3617..4807)
                     /locus_tag="PWY67_001957"
     CDS             complement(3617..4807)
                     /locus_tag="PWY67_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HDL2058217.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(5679..7310)
                     /locus_tag="PWY67_001958"
     CDS             complement(5679..7310)
                     /locus_tag="PWY67_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL2058218.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(7324..8997)
                     /locus_tag="PWY67_001959"
     CDS             complement(7324..8997)
                     /locus_tag="PWY67_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL2058219.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(9010..9327)
                     /locus_tag="PWY67_001960"
     CDS             complement(9010..9327)
                     /locus_tag="PWY67_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2058220.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(9352..10752)
                     /gene="lacG"
                     /locus_tag="PWY67_001961"
     CDS             complement(9352..10752)
                     /gene="lacG"
                     /locus_tag="PWY67_001961"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL2058221.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10989..11774
                     /locus_tag="PWY67_001962"
     CDS             10989..11774
                     /locus_tag="PWY67_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2058222.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            11777..12469
                     /locus_tag="PWY67_001963"
     CDS             11777..12469
                     /locus_tag="PWY67_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2058223.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(12743..13114)
                     /locus_tag="PWY67_001964"
     CDS             complement(12743..13114)
                     /locus_tag="PWY67_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2058224.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         4087 a         2716 c         2188 g         4321 t
ORIGIN      
        1 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
       61 ttaagataat taaaatatcg tttgcaatta gctttcttct tcgaaccttt tcacctgatt
      121 cagtaaagta caaaattgct aacttgaaaa ataaactatg attatttacc ttaattggtg
      181 atatatttat agcatcaaaa ttgcaatacg gtaattcttt ctttaaatac tcacccagtg
      241 aaaacagagg atagtgttga ggagcaagta cacaaatatc tttttcatga atgccgcttt
      301 caatcttttc ctttattata ttttttatta tgttgggtaa atcctcctta gtaactttgc
      361 aatcataact tataaaacca cggtcgtttt tattattccc tctgctttca atctcgtaag
      421 gacaactctg aaaattagag tagaagtcta ctaatctctg attagacctg taacagccat
      481 gtagcgtctt tgattgaaaa gtgtgactag ttagcatttc tagttcttca actgatttag
      541 ctataccatc taatgatcca taaattgctt ggtcaatatc tccaacaaac attgactgaa
      601 tttgagggtt gttttgaagt aatttagcta ttacattgta ttgtaattct ctagtatctt
      661 gaaattcatc cacataaatc gcccttatat tactagcaat attacgtgca acgttactgt
      721 ttacagttaa aattttatac gctactagta aaatcatatc aaaatcaatt tctttatttt
      781 tttccaacat agtgtgatac atttcagaaa cttgagtata ctgaacttca ttaattttaa
      841 aatcatgaga aagtgacaaa tcaattttgt tcagttcata cgttttgaga tttaaattca
      901 gcatattatt aatttggctg acgtaagctc tttgagtata atcatcaata attgtaaatc
      961 cttttgatac tcttgagaga ttcattttaa aaggataaat aataaattca agacaaaact
     1021 gatgtattgt ccccacccaa atattaatat catcatctat actaagtaaa cttaggcgtt
     1081 ccttaatttc ttcagcagct ctattagtgt atgtgatagc aattattctt tttagtgatc
     1141 catcgtgttt aattgcttgg taagcaattt tccttgtaag tacccttgtt ttcccactcc
     1201 caggacatgc atttacataa aggttatctt cactttccac aacatctttt tgttctaaat
     1261 ttaaatcatt tacgattttt tcgaagatat ttgtcatttt gaaacctcag cataaagtat
     1321 ttttgtgtaa ttttctatct gattgattag atacacaaca tcctcatctt taccaaagta
     1381 ctctaataaa gaaattattg ctatattcaa atcagctgta tcacttgttt ttaaactagc
     1441 tgataaagct gacaattctg tagtttcctc gtacaagtcg attgtatgga gtagaatttt
     1501 tttataaatt gagagatcta ctatttcctg acatgcaaaa gcaatagcat taagaatata
     1561 cttaggaatt ttgattaaaa agtcaatttt ttcacctaac tcaattgcaa accacccctt
     1621 accaacatga ttggctagtt ttaacatcgc taaatttctt tctaaatatt gttcactttg
     1681 tatttcttct ttataagcag ttattgtttt tttatctttg aatgcgtcat ctataattgg
     1741 actgacataa gttagaatat tttctttatt cacatctaaa aactcaattt caaatgttgt
     1801 attagcataa aaacaattaa cccaattgtt ttcattaaaa agggaggata atttttcttt
     1861 tcttgcttga cctttctctt gtgcagtgct tttatacaat cgatgttctg atgggattgc
     1921 ctgttcatct aaatcagtta caatagcaca ctttctctgt attctatcat cgtcaaacaa
     1981 gctagcgata tattcaaacg aagtactacc aacattaatc aaacctattc caagttcgtc
     2041 taagtttata ccaaataatt tttttactat ttgaggaata agtatttctt cagcatctcc
     2101 ttcaactaag ataacacctt tagaaaaaag caatccattt cttttaacat ctaaatatct
     2161 ctctactgcg tttattagat ttagattgtt cttttttaac ttttctttag caaatgagtt
     2221 cagattattt gctggattca taacgataga tttgccatta aacgatttta atacattcat
     2281 ccgagatatt tcagacactt cagctaaatg tacagaatgt gtggtcatga gaatctgagt
     2341 atagtttttt tgaagattta atccttcgaa cagagttttt tgaatatgat gatgaatatg
     2401 agcttctggt tcttcaatca acataatatt aagtagttct cttgaacgac acgcttcaaa
     2461 ttcgactatt ttcaaagcta aataaatcat atttagatgt cctaacccta gtaagtctaa
     2521 gtcgatattt tgttctggct tcattgaaag aaattttgct aaagaaccca tatcatctga
     2581 catagttgag gaaagaagaa gattaggaga atacactgaa ccaattatgc tatttagttg
     2641 ccgattaata ttttcgccaa ttttcctaat ttcaggaaca ctggtaatag tctcatttaa
     2701 ttgctgtata attgttttta atgactcaac attatctgat gatatttttg attcaattgt
     2761 ttccatgatt cttttgactg ggtttcgatt gtttttcatt tctctaagaa catctcgaag
     2821 agcgtcaaca taaacaaccg aaatatgctt gaaaatatct gacatgtcaa ttcttgagcc
     2881 caacagagct tcatcgtctt cttcaggatt actagctata ttgtcattga gtcttcctac
     2941 caatttatca taattttcat tgatacagaa atttagattt gatttagatg tgtagtaaaa
     3001 ttcatagtcg gatagacgaa tagaatttct aaattcgtta aattgatgag agtctaattc
     3061 agtggaaata tcaaataatt gctttctaat agctttgtta ggtctaataa atagagaaat
     3121 actacctaca cctttatctg catttgaaat caattcttct aagattgttt gactttcaga
     3181 atttaaagaa atggaagcac aaatctcatt gtcaaattct tcttcgctga ttccttcaaa
     3241 tgtcgcagag ataataatcc aatgcccttg ccacccattt tgaattccat gaaaaaaatc
     3301 tgattctttt agtgttttac tactatagta ataactatca tctaataaga ttctcaatgc
     3361 agtaagagca ttggttttcc ctgaatcatt ctcacctata atagtatttg tctcattatc
     3421 gaatcgaaag atttcggatt cataatttct aaaatttcga atttgaagag tagatataaa
     3481 cataagcaga tctcctaact atttaattaa tatgattata tagtaagttt atcataaatt
     3541 atgttagtaa aagttctcat tataagggag cgtcaataat tttgtgtaaa tgattcttcc
     3601 ctactgcaga ttgtttctaa ttctcaatca gttggcttag catttgttcg agctcgcctt
     3661 cggcctgttg gaaaccttta tgactgcgac ctaggaattt ttggttgtaa gtatcaaaag
     3721 acgagactaa gaaacgttcc atcgactctt cattttggaa ctgttccttg cggtggctgt
     3781 atttcttgat ttgtttatta aaggattcaa tcaagttcgt agaataaata ctcttgcgta
     3841 tggccaaggg gaaatcatag aaagtgagca agtgatcgtt cgaaagaatc gattcaacca
     3901 cttttggata gctggttttc catttttccg caaaagtacc acgtgcttcc aacgccacct
     3961 ctttagatga ggcttgatac accattttaa aatcatcaca gacttcctta cgatcatcga
     4021 cacgcacttt atgactgata ttacgggaaa cgtgtacaca acaatgttga aaacgagctt
     4081 tgggatagaa ccgactaatc gctcctacca tcccctttaa gccatccgtg atgaacagga
     4141 ggacattttt caaaccgcgc tcttgaaggt ccaataaaat ttcctcccaa atcgtgatgg
     4201 attcagtcgg tgcaatcgca tagctcaata cttcctttga tccgtccggg cgaatgccaa
     4261 ctgcgatatg aatagcttcc ttggcgacgg ttttccgctt taacggaata tacgttgcgt
     4321 ccatataaat agcagcataa cggtcatgaa gctcccgccc attaaacgcc gttacctctt
     4381 ctgtaaatga ttttgttata ttggacatgg tttggggcgt gtagtaatgc ccatacattt
     4441 tctcaatcaa gtctgcgatt tccgacatgg taataccttt tcggaagagg tgaatgacgg
     4501 tctcctctaa cgtgtcattc gtccgtctat aagcaggaac agtctgttgc ttgaactcac
     4561 cattgcggtc gcgcggaatc tggagatgaa gttccccata ctcggtcttg accgtacggt
     4621 cataggagcc gttacgagaa ttacccgtgt taaaaccaat gcgatcatac ttttcgtaat
     4681 ctaggaaagc cgttaactcc gttttgagaa gcgtattgac tgctttctcc aaatggacac
     4741 ggaataattc attcaaatcg cctttgttcg ctagagtctt tagaatttct gtagtaaaat
     4801 cgttcatagg gaagtcctct cttctgtgaa tgtgttgtgg taactctatt ctacagaagg
     4861 gacttccttt tttctattta cacaaaatat tttacactct ccattataac aattgggtta
     4921 aaaagaaaat agatagtatt ctacctcata tttatttgaa agtaatatat aaaagacgtt
     4981 caaattcgaa cgcctctcgt taaccaaata ttggattcag aatctagttt tcatatataa
     5041 acagagtatg attaggtgca aaaattgcac ttttattgca cttttatttt tatttatagt
     5101 gttttactgt tctgtatatt gttttaactc ccttgatagt atcgcattct gttttataca
     5161 ctttacgttg aaaacagagt gggaataaag agccaacata atatggaggg ggaatcccta
     5221 taaatcaaca ttcttcaata gctaaaatgc ttgattttca ttaaaaataa gatggttccc
     5281 taccaaaaga gactataata taagttaatt tgtttcattc ttatcataaa aagaagttct
     5341 cgaaaaacga actgttttcc gagaactttt ttctatccat ctaagtgctc aaatcatatt
     5401 cacgacggaa ttctaaggtt accctttctt aaacaaactt tatgttatct gcacaacttt
     5461 ttgcaatttt ttcattgctt cttacgaaat cgttgaactc tctttagcat aatacaaagt
     5521 gtagatgaag tctggtgctt tactcgtttt ggtaaaaatg actggaaatc gtttctggtt
     5581 atttttggca aaggcgaggt gcaggttcga ttcctgcctt ctacgctaac gatgtggctg
     5641 aagggaacct ccgatcttga atcgaaacga tccgcagtct attgtttcac caatgaaaca
     5701 aaatcaacgt tcgtccaagc atttcctgaa gccttgctat aaaccccaat ttcacaaaca
     5761 cctgttgtaa ttggaatgtc tttgattgta atttttgtcc attcacttgt tttaacaacc
     5821 gcagtattta gttccgctcc accataattt ttgatatata actgattagt atcaaactct
     5881 cccccagaca ttacataggc actcaatgtg taagttcctt tgcttatgcc actaacattt
     5941 tgataagctg atgcctcgta tgcttgatcc tgccaaaaag ttagtttgta gtccccaaaa
     6001 aaagtgcccg cttccgtttt gatcgtaccc ttagcggttc cttgttgaaa ccaggtttgc
     6061 caattggaag gactggtcgt tggtccatcg ctttcgaaac ttgcattttt aactagattt
     6121 atttgcgccc ctgcttgttt aaacgcacgc aagcttggca aggcgtttcc agcaaaatca
     6181 aacatcgctt ggttgtccca ttcgtttccc atagtggatt gaacactgag ataattcata
     6241 cctgcttgtg ttgcccaact cacatttccg ttgtaccaaa gaggttccca atagaagaag
     6301 cccgccccgc gattattcgg tactgcctga atccgatcgg caagatccag caaatactct
     6361 gcttgtcctg tcggtgttgg ttcatatccc cccgcagctg cttcggcttc accaaaagca
     6421 tttggcatag tatcggcatt cgctgttgta tgcccataag ctgtttcaac tacgatcact
     6481 tctttaccat accgttggct gatcgcattc atattggttt gaagttcatt cattgaaccg
     6541 tgccaatacg gataatacga cactccaatt aaatcaaatg acacacctcg actcgtgatt
     6601 tcatcaaacc accaagtaaa tgctcctgta tcgccaccat cagctaagtg gagcattact
     6661 ggagttgttt taggttgagt gtctcgaaca gcttggatac cagacttcaa aaaagcagcc
     6721 aatcgatcaa attctccccc gccttctccc caagacttgc cataaggcca gagcattccg
     6781 ctattcagtt cattgccgat ctgaaccata tcaggataaa cgtccagctg cttcatttcc
     6841 gacagaacgt tctttgtata gtcgtacaat gccgtattca tttcttcaaa ggtcagacct
     6901 tgccatgctt taggaagatt ctgtttccca ggatcaaccc aaaaatcact gtagtgaaaa
     6961 tccagcagca ccttaagtcc cagatttttt gctcgttttg caaggtcaat cgtcgtttcc
     7021 agatcatttg tccctgctcc ataactttct ccattactat tataaggatc ctgccacaaa
     7081 cgcagtcgaa catagttgac accattattt tttaaaatct ctaaggcatc ttttgaaata
     7141 tcgtcttcat aatattttgc accagatttt tccatatctg acaggatcga aatatctgct
     7201 ccgcgaataa attcgctggc agatgcttca acagtttttg aaacagcccc caaagcccct
     7261 aacacagctg caaataaaac agctttacgc aaccaaattc ccattttcat tatggctcct
     7321 cctttattga aattggttca gaataaactg aacagctttt tcaggatctc tcgttaatcc
     7381 gatatattct gctccttttg tcgcaaccag cttgattcct aagcgatcgg tgtcttcttt
     7441 gatatcatca tagtaagaat tgacttgcgg tgctaagacc accatatcaa aatcctgcag
     7501 catttcgtaa tgagaaccat aagcacccgc cattgcactg atcggtgctt tcgattcaga
     7561 tgcacctttt tttaaagcat tagctaacat cgcacttgtc cctgcgccag cacaaagtac
     7621 caacacattg atttctttat ttgcatcgct gataacttga ctcgctgcca caggtacttc
     7681 ctcacgtaca tcggcagaag caccttcctc ggttgcgatc acctctgctt tttgtgcttc
     7741 tttctctact aagacagcat cgtatgcttt gcaaaatggt agatagatca ggaagtcaac
     7801 gacgattaga accgctgcta gaacaaaagc taagataccc atgcccgttc ctagaaccaa
     7861 accgatcggt gctggagttg cccaaggcaa aacgtacata aagctgttca tgcccaatac
     7921 atcgacaaaa aacttgaaca ggcaaacgtt tacaacaggt gttaacaaaa atggaataaa
     7981 aaaataaggg ttcaaaatca tgggtgccgc aaatagcaat ggttcgttga cagcgaaact
     8041 tactggaata aaggaagctt tcccaactgc cttcaactgc ttagacttag caaagagtag
     8101 gaagataaaa ggaacaacca atgttgcccc agttcctccc atagttccta caaaattccc
     8161 taaacccacc gtcagtactt tagaagcttg ttcaccggaa ttgaacaatt gcagattggt
     8221 ttccacattg gcatagataa ttgccgcaat tgccggctcc acgatcgacg gtccatgaac
     8281 accgacaaac caaaacagcg ccatcgctcc ccagataata gcgattccta agtacccgtc
     8341 agctgctgta aatagcggct gcagcaccgt aatgattgct tctgcaaatg agatttggaa
     8401 gatttgtcga atgcctaaat caattaaaac ggccgcaaat actgaaaaag agaaaggaaa
     8461 aatatctcgg aaggtttgcg aaatcgttcc aggaacttct ttaggcattt taatggtaat
     8521 atctttcagc acacaaaact tatataaatt aactgttaaa aatgcagcga caaaggaagc
     8581 cagtagtccc tttgtcccca tatattccgt tgaaaatcca ccttcaatcg gagagacgct
     8641 taacaacagg aaacttacca cagaggccag catcacagag gtggtgttga ttactttgtt
     8701 tttcggtaag cgacgattca tcgaatcagc caagcagcga gcagtggttg ctgaaacgag
     8761 taaccctaca acacccatcg aataattata gactttccat agccatgtgg agacagattc
     8821 aggccattgg taaccaaaaa tctcaggaac cgcagcaatc aaaatgaaaa tgcttgaaaa
     8881 taaaatagct ggcatcgctg ttaaaaatcc gtcacgaacg gctcctaaat aactattacg
     8941 tgagattttt tcaaaaaatg gtttcccttt ttcgatttgt ttaatgattc catccatagc
     9001 gttcttctcc tatccttgat tctgtctgta cagtacaata aagtcttgaa tcagatctct
     9061 cagcaataaa gtggtcatta aatgatcctg cccatggata aaaatgaatc ccatctccat
     9121 ctctttccca gatgcttctt ccgctaaaat ttttgtttgc gaattatgtg caagggttaa
     9181 attttcgtcg gcttctttca acgccgaatc aacatgttca aagtttcctt gtcttacttc
     9241 tttcaataat ttcaataatg tactgcgagc gtcgcctgaa tatgcaacga tctcaaaacc
     9301 aagcatttgt aattcctctt tattcatctt ttcttcctcc attatcactt attaaataat
     9361 cgttttggtt tcactgacca acttgtacca gtaagctgat tcttttgggt aacgcttctg
     9421 ggtttcaaaa tcaacataaa acaagccata ccttttgtta tacccatttg tccatgagaa
     9481 aagatccata agcgaccaca aaaaataccc cttcacattt accccggctg ttatcgcctt
     9541 gcttaaggat tccaaataga ctctcaaata gtcgattctc ggctgatcca taataatgcc
     9601 atcttcaaac tgatctttat accccatccc gttctcagtg atatagattt tgttgtaatg
     9661 tgggtaatcg cttttaattc gcagcaacaa gtcatataga ccttctggat aaatcagcca
     9721 gtcccaatca gttcgaggaa ttccttcttt ataaatacgt tctccgattc ccttcacttt
     9781 ataaacagag gtcccttttt cacccgtacc gttatgatgg attgcatttt ctccatcgta
     9841 agctttgaca aaatggcatt ggtagtgatt gatccctaag tagtcattac gcgtggatgc
     9901 ttttttcaat tcaacgaaat cttcttcagg aaaatgatag ctggcctgat ttgcttcaca
     9961 tatctcatca agagcagtca aggtttcagt tgaataatag cctaaatagg tagcatctaa
    10021 taagaaacgg atcgacaatg catcgtctaa aaaggcagca tgcttatctt ccggcgcgtc
    10081 tgttgccgca tatttcgtct ctaatgagtg aactacacca atttcacccg gaagttcgtt
    10141 ttctttgaaa tagttcacta cacgggcatg agcaaccatc atattatgaa gacaggcgac
    10201 gatctttgta aaatcatatt tgattcctgg tgggaaaaca cctaacaaat attgattggt
    10261 tgcaactgga tagatttcat taaaggtact ccacaccttg acttctttga attcatgaaa
    10321 acaaaaaatg gcataggaaa caaatgcttc tattgtttct cgattcaaaa aatctccatg
    10381 gtcaaacaaa cgtttagggg tatcaaaatg atgcaacgtc acaaacggcg ttacgtttct
    10441 tttcttacat tcttcgaaca cacgatgata aaaagcgatt ccttctggat ttggtttccc
    10501 agctccatca ggaaaaatgc ggctccaagc aatggataat cttagaccat ttacaccaaa
    10561 acgttcacac aattcaatgt cctttgcata ttgatgataa aaatcacttg ccggatcggg
    10621 actgaatcgc ccttgttcct ctaaaaagtc atcccaagca acagcgcctt tgccaccttc
    10681 cttagtggct ccttcaacct gataggctgc tgtggctccg ccaaaaataa aatcttctgg
    10741 taatttaagc atgtacatct ctcctcaaat ctataatcag ttttcgctct ttctaactga
    10801 ttgcattgta aacgcatcca aaacatttgt caacaaaaac aaacacaaat aatcataaaa
    10861 accagaaaac agcaataaaa tctattgtaa caaacataaa aaccacccta aatgtttact
    10921 tttgttcgaa ttagattgac tgttgtccgt ttatatgata gattatcctt aaattagagg
    10981 tgaagacaat gttgaaaaaa gaaagattgc ttactatcgt agaaatggtc aataaaaaag
    11041 gaatccttac cgttaatgaa atcatcaatc aattagatgt ttctgatatg accgttcgca
    11101 gagatttaga tgaattagaa aaatcgggca agctgctgcg tgtccatgga ggggctcaaa
    11161 gtctttccta cactttagat caagaacttt ctcacactga aaaagccact ctgcagattc
    11221 aagaaaaaaa agaaattgct gccacagcag cctccttgat tcacgaggga gaaacgattt
    11281 tcttaggacc aggtacaaca atcgaactat tggcgacgca tttactgaac aagaaaattc
    11341 ggatcattac aaataattat ccagtttttg atattcttgc tcaagcagat ggaccagaaa
    11401 ttctactgat tggcggtgat ttccggaaaa ataccggcgc atttgtcgga ccgatcacca
    11461 acgacaattt aagaagacta aagtttacaa aagcatttat cagttcaaat ggcgttcaca
    11521 acgaagcgat ctctacttac agtaccgaag aaggagaagc acagcagatc gctctaaata
    11581 attcccgtaa gaaatatcta ctcgttgata ataaaaaatt caatcgagat gatttttatg
    11641 tgttttataa tttgcatgat ttcgatctgc tcattacgga tcgccagatc tcgcgggagg
    11701 ttcaagagca ctatgcacaa tatgtcgatc tgcaaattgc tgattcaacc gaaaatacca
    11761 ctataggagc gtaactatga aagggattat atttgatttc aacggaacaa tgtttttgga
    11821 ctctcatctg catgaagctg cttggctgca tatgatacat gatcatacaa aagatggttt
    11881 atcagacgag gatattttaa aaaatattca tggtcggacg aatacggaga ttttgaaaca
    11941 tttcatttca gagcatttga ccgcctctga gatcgccgta ttgtcagaag aaaaagaaag
    12001 ctattatcgt atgctttgct tgcaaaatga aaacgagctt cagttgacaa aagggcttga
    12061 agcgactctt gaccagctga ctgaccaaca gattccatta acgattgcca cggctactgt
    12121 caaagagaat gttgcttttt attttgatat tttcgattta gcccgctggt ttgactggga
    12181 caagattgtc tatgacgacg gctctttccc tgggaaaccg caaccggata tttttcttaa
    12241 agcggcagac aaactgagct tagctccggc tgattgtctt gttatcgaag atgcttactc
    12301 aggattactt gctgccaaac gtgcggggat cggaacaatt attgcaattg atccattcgg
    12361 taaaaacaaa acagtatttg aacaggcaca gctaaataaa gatggaacca tcaaagattt
    12421 tactggtttt tggacagagc acctgtctgc cacatccgtt aattcttaaa cgattcaact
    12481 aactaccaaa gaaaagcaac tgaaatgcac acctcctttt ataaaaaact gccagtgaaa
    12541 cgacatgaaa gatacactgc tatatcctta gtgaaatata gcagtgtatc tttgactttt
    12601 tcattctttt aaccatatga cgcgttcatc tctatctata attaacatta aaaatagtgt
    12661 tttcacatta tttctcaaac caatcttaaa aatcatcagg agtacttttt tgatatactt
    12721 ttattaatgc tgggcgtaat ggttaaaaga tttccactag ttcctctcca ccaagctgag
    12781 ttgaaaaatg cttatctgta aacaacatag acaaagagga aataaacgtt tccgtatctt
    12841 tctctgtccc aaggatatta attaattttc ctactaggta agtatcatta aacttaagat
    12901 ctgccttcac atcaaaagtt ttgataaaag cttttatcat gcgtacaatt ttgtctttat
    12961 cgccataatt gatttcacca ctcatatcaa tatagtcact aatccctgaa gaaataatgg
    13021 caactgcttc ttctttcgcc ttatcagata attcatttaa tgactgggat tgattgatga
    13081 cttcttgagt tttatctgtt acgtaatctc tcattatcct atcggcatac tccgcaaagt
    13141 aatcttgaaa aaattcttct acagggtaac caacttcgtt ttcccaacca ggagaaattt
    13201 cattatcagt acttattaaa tctattaaaa accaacccgt tctctgagtg ttgattctat
    13261 aatattttta atttcctata taaatcaaaa atattataga atcaataagc ta
//
LOCUS       DANKAZ010000069        13254 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000069 DANKAZ010000000
VERSION     DANKAZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            143..1882
                     /gene="yfcC"
                     /locus_tag="PWY67_001965"
     CDS             143..1882
                     /gene="yfcC"
                     /locus_tag="PWY67_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2058225.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2039..3223
                     /gene="iadA"
                     /locus_tag="PWY67_001966"
     CDS             2039..3223
                     /gene="iadA"
                     /locus_tag="PWY67_001966"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL2058226.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3334..3537
                     /locus_tag="PWY67_001967"
     CDS             3334..3537
                     /locus_tag="PWY67_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2058227.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3638..4300)
                     /locus_tag="PWY67_001968"
     CDS             complement(3638..4300)
                     /locus_tag="PWY67_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2058228.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4313..5815)
                     /locus_tag="PWY67_001969"
     CDS             complement(4313..5815)
                     /locus_tag="PWY67_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2058229.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6363..8042
                     /locus_tag="PWY67_001970"
     CDS             6363..8042
                     /locus_tag="PWY67_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2058230.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8169..8648
                     /locus_tag="PWY67_001971"
     CDS             8169..8648
                     /locus_tag="PWY67_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL2058231.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8867..9547
                     /locus_tag="PWY67_001972"
     CDS             8867..9547
                     /locus_tag="PWY67_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL2058232.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9555..10886
                     /locus_tag="PWY67_001973"
     CDS             9555..10886
                     /locus_tag="PWY67_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2058233.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10901..11863
                     /locus_tag="PWY67_001974"
     CDS             10901..11863
                     /locus_tag="PWY67_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL2058234.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11974..12312
                     /locus_tag="PWY67_001975"
     CDS             11974..12312
                     /locus_tag="PWY67_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL2058235.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12391..13098
                     /locus_tag="PWY67_001976"
     CDS             12391..13098
                     /locus_tag="PWY67_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL2058236.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4219 a         2337 c         2907 g         3791 t
ORIGIN      
        1 cataggagaa aaaaaacaaa atagaaaaaa gtgattttct aataaattat tcatgtggaa
       61 atgtcatttt tactgtataa ttcatttgat tatccatttt ttattcatca aaattactga
      121 aaaaagaaag gaagaaataa atttgaagaa aacaaattct tcaggaaaac cgaaacgcga
      181 cttcactaaa ttatcgacac cccatacgta tgtcattatt tttggtgtag tcatctttgc
      241 atggatactg acttttgtcg ttccagcagg aaagttcagc acgcaagata ttgaatacaa
      301 agatgcaaat ggcgaaacaa gtactcgtac tgttttacgt caagattcct tccgttatgc
      361 ttacgagctg gataagtcgt atgtgtttga tcaattagaa gaactgcaag atcatccagc
      421 agaacgtgaa aaactggatg ttccggaaaa aggattggaa aaagtcattg ctgacggaga
      481 aaaaaatctt acgcaggaaa aactagatga aatctccttg acagatgatg tgctttacga
      541 tcagtacggc gaaaatatct atgatacttc taaaaaattg cataaaacag ccaaaatatg
      601 gggaacagat gattttggcg gctttggttt cttaaatttt gtttttgaag gactagtatc
      661 tggagataag tatggatctg ctgtagggat cgctgcattg atccttgttg ttggaggtgc
      721 ttttggtatt attatgcgta ccggagcgat cgacgcaggt atctatgcgt ttatcagtaa
      781 aactaaggga ctggagcgtt tagctttacc gctattattc tttgcttttt cattcggtgg
      841 cgctacattc gggatggcag aagaggtaat tccattctca atggtgatgg ttccatttgt
      901 cattgcgtta ggatatgact cgatcgtagc agtaaccgtg acttatgtcg catctcaagt
      961 cggaaatgcg acctcttgga tgagcccgtt tagcgtagcg gtggctcaag ggattgctgg
     1021 aatccccgtg ttgtctggtg cgacattccg attgattatg tgggtagttg ttactgcatt
     1081 agccgctggt tatatgatga tatatgcaga aaaaatccgt aaaaaaccag aaagttcgtt
     1141 gacttacaaa tcagatgatt atttccgttc tcatatcaaa aagacatctg atgaaaataa
     1201 aaaattcatg cttgggcata aattgatctt attagagatg ttagttgtcc ttgtctggat
     1261 cgtttggggc gtgactcaaa aaggctatta cattccagaa atcgcttctc aattctttgt
     1321 gatgggatta gttgctggaa tcattgctgt tcttttcaaa ttagatggaa tgaaagtcaa
     1381 tgacatcgct tcttctttcc aatctggtgc agctgattta gctggtacag caatcgtagt
     1441 aggtatggcc aaagggattc ttcttgtctt gggaggatct gatgcatctg ttccttcagc
     1501 tttgaatacg attttgcatg ggatcgggac ggcgttgacc ggtgttccag ctgtcattgg
     1561 tgcttgggcg atgtacatct tccaaagttt gttcaacttg gttgttacat caaattcagg
     1621 acaagcagct ttgactatgc caatcatggc tccactggca gatttagtag gtgtatccag
     1681 acaagtagca gttcttgctt atcagcttgg ttcaggtttt atggacgctt ttacacccgt
     1741 atcagccagc ttgattggtg tgctaggtgt tgcacggatc gaatgggcaa aatgggcaag
     1801 attccaaatc aagatgcaag gattcctgtt tgttttagga actattttca tcatgattgc
     1861 cattgcaata ggtctgcagt agtactggag acctgtaaat aaaaaagtaa tccaaacagt
     1921 agataaggac tccaagaacc gtcattttac ggtaaaatca aaggagtaaa gatcctatcg
     1981 tttggattat tttttaagat cctatcatca gttatactta aagaaagagg aaaaatgtat
     2041 gaaaatcatt cgacaaattg aagtgtttgc accagattac ttaggaaaaa tggatgtact
     2101 gattgccggt gggaagattt tggctgtaga agaacagcta gaaggcggct acgaaggagt
     2161 ggaagtagaa gaactttcag gtgagggaaa agtactaacc ccgggtttca ttgactgtca
     2221 tttccatatt ttaggtggag gaggagaagg cggttatcaa aatcgaacgc cagaagtcac
     2281 attgagccaa ttgactacag ctggtgtaac gacagttgtc ggttgtttgg gaacagacgg
     2341 agaaggtaga gatatgactg cgttgatcag caaagcgaaa ggtctggaag cagaaggaat
     2401 ctccacttat gtatacgaag gttcgtaccg attgccggta aaaacagtaa cagattctat
     2461 catcaaagac tttttgacaa ttgataaaat tatcggaatc ggagaaatcg cagtatccga
     2521 tcaccgttct tctcagccaa gttttgaaga gttcgctcgt gcagtagcag atgctcgtgt
     2581 aggaggcatg ttatccggaa aagctgggat catcaatgtc cacttaggcg gaggaaaaag
     2641 aaagctagag ctattgacac gaatcgtaga agaaacagaa attccgatca cacaattttt
     2701 gcctactcat gcaaatcgca caccagaatt atttgaagct tgcgtggcat ttgccaaacg
     2761 tggtggcacg attgacttca ctgctagcga agatcctgat ttttgggaga agacagatgg
     2821 tgaagttcgc ttcagcaaag cactgaaacg attgatcgag gaagatgttt ctttagataa
     2881 tttcacgatg acctcagatg gacaaggaag cttgccttat tttgatgaaa acaatcattt
     2941 cctagggtta ggagtaggaa gtgccaaggc attgctagta gggatcaaag aagctgtgca
     3001 aaaagaaagt atccctttgg aaatcgcctt acgagctata accagtaatc cagcacgtat
     3061 attgaaattg gataaaaaag gaaaaatcga aattggtgct gatgcagatc tgtgtatctt
     3121 ggataaagaa acgcttgata ttgatacagt tatcgctaaa ggagaaataa tggtccaaga
     3181 aaaagaagtc aaagtatggg ggactttcga aaaaagtttt taatcaacag ctctaactat
     3241 cttttttaga aaacgagcgt tctgtttgtc tgtatctgtt gttgatgtta ccctgaaggt
     3301 agatcaataa acaaactaag aggaggaagc acaatggcag atttaaaagg acgttctaaa
     3361 gatatgaaag ataaagtagt cggtgaagga aaagaagcat acggcaaagc aacagacgac
     3421 aagagcaaag aaacagaagg gaaagcacaa tcactaggtg ctgacatcaa agaaaaagct
     3481 cgaaatttag gcgacgaagt caaagacaag ttttctcgaa aagacgaaaa aaaataaaaa
     3541 ccacttatta ctaattgaat caagcaaata aaaaatccga accatatctg cgagacagat
     3601 atggttcgga ttttttattt gatcattatc ctgtttttta aacttcgatt ttttgaaatt
     3661 ttttattttt tagttcaaag acttcatcac agctttttgc tacttcaatc tcatgagtca
     3721 cgatgataat acagcgattt tgttcatggg cgatttcttg gaaaagtttg acgatatctt
     3781 gtgaagttgt ttcatctaga tttccagtag gctcatccgc tacgattagt tgatgttcac
     3841 agcagatagc acgaacgata gcgactcgct gctgctgacc tcccgaaagt ttggtcacct
     3901 gtttttttgc taatatttca tcaattccta cacgagccag tcgatctaaa gcaacttgtc
     3961 gataattctc ttgcttggtc ttagtgattt ccattgctgc tatgacgttg tctagagccg
     4021 ataaataagg aagcaagttg taagcttgga aaataatcga gacatcattt ttacgataac
     4081 ttcttaaccc gattttgttc agtgaacgat cttgatagta tacctttcct tcttttggtg
     4141 catctagtcc agaaataagc gaaagaaaag tcgtttttcc agaaccactt gctcctaaaa
     4201 tagcgtacat ttttccagct tcgaaattca gttctacatc tttataaagc gcatcttgcg
     4261 gatcttgata ccaaaatccc aattgttctg tccgtaacat caaattcctc ctttagttta
     4321 ataggatttt tcttgggttt aatcttaata tccctataga agctaaaaag acagaaaaca
     4381 gactgattcc aaatgctact ccggctaata gtcccaattg tgcaggtttg acattgattt
     4441 ccaattcgct gacttgatca gaaacagcaa atggattatt cgtggttcct tgttgaccat
     4501 cgcgttgacc gccttgttct tgtctgttac ctggcatttg tccaccgcca ggagcttgct
     4561 ggttttgtgt cgtggtttct gtctgttggt caagtagctg ctgtcccacg gcattcgcga
     4621 cgacgtttcc gctaagcgaa gcaactccta atgcaagaat catacaaata gccacttcgg
     4681 taaacaattg gagaatgatt ttacttctgg attctcctaa agatagcaag acgcctaact
     4741 catgccgacg ctcccgaatg gtaatcataa tgatcagagt aagtatgata ataccggcta
     4801 cagccactag taaaacaaca tttttagaaa aacttgccac gttgtttaaa ggttccaaca
     4861 ttgactgata catggaatca tttgattgta agctaaatgt atctgtgtcg atcagcttct
     4921 cagcttcttt gagaaactca tccattttat ctggatcaga tagagtgtaa atagcagaat
     4981 ctatcgtgtt atcgtcagat gtcccattta gttcatttgc aaaagtgtaa gaagtgtaaa
     5041 gtgtattcga cggattcatg aagttgaatt tcatacccat actgccgctt gtttcgctgg
     5101 attcataaat tcctttgatc gtcatttcat aagtggtatc ttcatcttca gtactcgtga
     5161 ttgtgaaagt atctccgact gccaggtcat ttgtttctgc tagagtagaa tcaatgacta
     5221 cattgtttgt gcctttgtcg ctgtcatcta ttccttcacc atccgtgatt tttgctgttc
     5281 catcagaaaa attactattt tgtgcagttg ctgacacacc actgatttga aagtctgctt
     5341 gagtcatttg cggattcccg cccattcctc cagcaggttc accgcctgca tcagtagttt
     5401 ctgaactgtc ttcacttgaa tcagaactag tgatggcagt tattccatct tttgccatag
     5461 ctgaggcgga actttcaaag gaataactct ttacattatc taattcagct attttttccg
     5521 cgtccgttaa ggaaactggt gtaagagaaa aactgcctgg atctggacgt tcttcagtgg
     5581 tagaaccgct tgaagaagtt tgttcctgtt ttttaaatga agcttctcga ttggcactta
     5641 aagtcactgt tgctccaact tctttttgtg cttgattcgc tgcttcttct gcagcgcttc
     5701 gaatcgtgag gccagctagc acaaaaataa gaatggctgt gaacacggca aacaataaca
     5761 atgaacgtcc ccattttact tttgtacttt tccatgcacg tttcataaaa ttcatatttt
     5821 gcatccctcc tagtagatgc atttgataat agacaaccta tcttaaagct agcttaaatc
     5881 ttatgctgtt tgcacaccat caacatgagg ttctgtatat ataaaaagtc actatagtta
     5941 ttgcaaaaaa tgtggtaaaa tgaaaaaagt atgtttcaaa ccgacgaaga agaaatgaaa
     6001 gaggaatagc aaaagaaatc ctagatgcta cagagggatt tttacgtgca tagttgttct
     6061 aacaattgct gatactagcg atttagttta tttgttgtat aataatgggg aacagctgaa
     6121 tacttatata agtattctgg ctatagcgtt tcgcaaaaga tccattttgt taatgaagta
     6181 tatccaaaat aaaatgccca tccaactgtc agaacgcgga tgtgctggta attttcctgc
     6241 tgattctcca taagttgaga aatagtgatt ttgcatatgc ttcattggaa tggctcatat
     6301 atttagataa agaagaaaat cgacatcttt aaacttggat gtcataagaa gaggtgaagt
     6361 ttgtgagtac cattaaaatt atcccattag gcggcgttcg tgaaaatgga aaaaacatgt
     6421 atgtcgcaga agtgggcgag gatattttta ttttagattg tgggctaaaa tatcctgaaa
     6481 atgagttgtt ggggatcgac gtcgtaatcc ctgattttac gtatttagaa gaaaacattg
     6541 atcgtgtagc tggtgtcttt ttaacacatg gacatgcaga tgcgatcggg gcacttcctt
     6601 acttgttgtc aaaaatttct gtaccagtat tcgggacaga gttgacgatc gagttagcaa
     6661 aattgaatgt cagtcgtaat gaagcagcca aaggatttaa ggatttccat gtggtagatg
     6721 agcatacaga aatagacttt ggtgaaacag tagtgagctt tttccgtacc actcatacga
     6781 taccagattc agtggggatc aatctgaaaa caaaagaagg tagtatcgtt tatacgggag
     6841 attttaaatt tgaccaaacg gctatcccaa tgtatcaaac agattatgca cgattggcgg
     6901 aaatcggcaa agaaggagtc ttagccttac taagtgaatc ggcaaatgca gaaaatcctc
     6961 agcctgtagc ttctgaagcc caaatagcag atgaagtatt tgatacgatc aaatattggg
     7021 aagggcgtat catcgtcgct tgtgtagcaa gtaatttgca acgagtgcaa caagtattgg
     7081 atgcagcaga aaaagcacaa cgaaaagttg tcttgactgg tcaagatttc gaacgaatta
     7141 ttcgcacagc gatgcgttta gaaaaacttc aattaccaag tgaagatctg cttgttacgt
     7201 tgaaagacat gaaaaactat gatccagaag aactgatcat tttagaaact ggccgaatgg
     7261 gtgaacccat caagtctctg caaaaaatgg caaacggtac acaccgtaat gtcaagattg
     7321 aagaaggaga tttggtttat atcactacta cgccaagcat cgcgatggaa accatcgtgg
     7381 cgaaaacgga agatatcatt tatcgagctg gtggaacagt gaagttgatc tctgagaata
     7441 tgcgcgtttc tggccatgcc aatcctaatg acttgcaatt aatgatcaat ttgatgaaac
     7501 caacatattt tgtgccagtt caaggagagt atcgtgaatt agctgctcat gcagatttgg
     7561 ctcatgcagt agggatgcca tacaaaaata tctatattac tggacgcgga gacgtgctgg
     7621 aatataagaa caagcgtatg agtgttgcag gaactacaag tgcagaaaat gtaatgattg
     7681 atggattagg cataggtgat atcggaaaca ttgttttacg tgatcgaaaa atcttgtcag
     7741 aagacgggat tttcgtagcg gttgtcacta tcagtcggcg agaaaagaaa atcatttcaa
     7801 aacctcaaat tacttcacgg ggattcgttt acgtaaaagc tagccgtgat ctgatgaaag
     7861 aaagcagcaa tatgattgaa acaatcgttg agaaacattt agaaagcaat gattttgagt
     7921 ggagcaaatt aaagcaagac atccgagatc aattaggccg ttacttattt gaacaaacaa
     7981 aacgccgccc agtgatcttg ccagtcatta tggaagctac tcaacgtcga aataaaaaat
     8041 aaacaaaaag agtaagatga acctctctat agagtgctcg tcttgctctt tttttgcgaa
     8101 aaacaaataa tttttgaata cagcatccct cgctttataa taaacgatag aataaaggga
     8161 gtgaatcaat gatgagtcaa acaaaagtcg aacgttattt agaagaacaa aagatcagct
     8221 atcaaccact tgattttagt catgtggtag aagaatcttt cacagaagaa ctacaaaaaa
     8281 gagggattga tccagctttg atatgtaaaa ctctcgtagt gaaaggaaac aaaactgggg
     8341 tgatcatcgc attgattcct ttgaacgatc acttagatta caaaaaaacg agacaagtga
     8401 cgaaagatcg taaagttggt tttcctggaa tggattttgt attaatgcat acaggatatc
     8461 cccacggagc aaacacccct attggaataa aactcgcaca tcctgattat tgttttttag
     8521 cagacagttc tttaaaagaa aaagaagaag taattgtgtc aagcggtgaa atcgggagaa
     8581 gccttcggat aaaagtagcc gatttagaaa agatcgtcca tcctatttac gctgatttaa
     8641 ttagttaagg gacgagaaga gaagctaagc agagcagaga cctcaaactt gagattatat
     8701 ctgcttggta cttggtgcat cctgtattat ctgttacaat tgctttgtac atactgtact
     8761 atcaatccaa tgaaaatata tttagcagct atattttttc attgatgtgc aagtgatgaa
     8821 atcgttttta ataaaaaaga tacagagaaa acgcgaggtg taaaatatga aaattacgta
     8881 tcatggacat tcctgtttaa caattgaaat ggaaaatggc caacgcgtga tgattgaccc
     8941 gtttattaca ggaaatccat tgacagactt aactgcagaa gaagtaaaga cagattggtt
     9001 gctgattact catggtcata gtgaccacat aggagatatg attcctattg caaaagaaaa
     9061 tcaagccaca attatcagta ttgttgagat tgctgattat gcgaacagca gaggagtaga
     9121 ttcattcggt atgaatatcg gtggaaaaca tgcgtttcca ttcggcgtgg taaaattcgt
     9181 acatgcacag catagttcaa gctatgaagt agacggtatc gttcagtata tgggcgagcc
     9241 tagcggaatt attattcaag cagaaggaaa aacgatttat catgcagggg atactgctta
     9301 tttcagtgat ctaggattgc ttgcagaaga attcgatatt gatgtcgctt tcttgccgat
     9361 tggcgataac tatacgatgg ggccagaaga tgcagcaaag gctgcgcaaa caattcgtgc
     9421 caagaaagta gttccaatcc attacaatac tttccctgtc attgaacaag atcccgaatc
     9481 ttttgtagag ttactggaag atagtgaagg aaaaataatg agcgtctcgg aaacgctgga
     9541 agtgtaggca gaaaatggcg acaaaacatg atttgatttt agaatatatt gaaagtttac
     9601 ctgttggcaa tcgaatttct gtaagaagca tcgcaaaaga attgaatgtc agtgaaggaa
     9661 cagcgtaccg tgcaatcaaa gatgcagaaa atgtcggact agtttcaaca attcaacgag
     9721 tagggaccat ccgtatcgaa cgcaaattaa aaaaacatat tgaacgtttg acatttggcg
     9781 aggtcgtcag gatcattgaa ggggacgttt taggcggggc ggcaggatta gacaaggtct
     9841 tgaataaatt cgtcatcggc gcgatgacta aacatgccat ggaacgctat attacgccag
     9901 gatcactaat gatcgtcggc aaccgggcgg aagtccaaaa attagcattg gaagatggcg
     9961 ccgcagtatt gatcacagga ggattcaata cgactgctga gattgcaaag ctagctgatg
    10021 atcttgaaat gccgatctta cgcacctctt atgatacatt tacagtcgca accatgatca
    10081 accgagcgtt aagcgaccaa ttgatcaaaa aagatatcat gcttgtcagt gatatctata
    10141 tgccagctga aaagactcat tatttgcagc agacagatac gatttctgat tatcagcgtt
    10201 tgtcagaaga aacgcagcat tcgcgttttc cagtcgtcaa ccgccatcat cgtttgatgg
    10261 ggattgttac ggcaaaagat gttttaggga aatcgccaaa ccagatcatc gatcgggtga
    10321 tgacgaaaga accaatcagt gtaaagaaaa caatgagtat tgcctctgtt agccatcaaa
    10381 tgatctggga tgggctagaa gtcatgccgg tcgtttccga tgacttgaca ttggaaggct
    10441 tgattacccg gcaagatgta atgaaagcga tgcagcttgt ccagcgacaa ccgcagattg
    10501 cagatacgat ttcggatcag atatctggaa atatccatac gattgatact gaccgtgaag
    10561 gaaatcgttt ggaaaaccct aagttcaaat tcgtagtagc accacaaatg gtcaatggcg
    10621 ttggaaccat ttcttttggc gtacttagcg aaatcatttc tgatgtcgca caaaaaacaa
    10681 tggtgatgaa ccaaaaacga aatatcttga ttgaacaagt gaacctacat tatttgcggc
    10741 tgatccaatt ggaaagcgaa ttagacattc gtccacgtgt attggaaatc ggcagacgtt
    10801 ccgctaaact ggatatcgaa gtgtttattg aaaacaccat cgtttccaaa gcaatcgttg
    10861 tttgtcaagt aatggaacgt tcttagaaag caggagtgaa atgacagtac agcaagaaat
    10921 agtagataca ataaaaaaat acgataaaat cattatccac aggcatttgc gccctgatcc
    10981 tgatgcatta ggatcacaag taggattagc cgaactatta cgggaaagtt tcccagaaaa
    11041 agaaatacgt caagtaggtg aaacgattga agggcttcgc tttttagcag aaatgcaaga
    11101 agttgaagat gtcttctacc aaggagcgct agtcatcgtg actgatacag ctaattcacc
    11161 cagaatcagt gatcaaagat atcagttggc agaaaaactg atcaaaatcg accatcatcc
    11221 caatgatgac ccttatggag atcttgtgtg ggtaaataca aaagcaagta gctgtagtga
    11281 gatgattgca gaatttgctt tgatgtttcc tgaatcttta aagatgaacg ttaaggctgc
    11341 acggttactt tatgcaggaa tcgtcggaga tacagggcgc tttttgtatc cctccacgac
    11401 gtcccgtaca ttagagattg cttcgatatt gagaaattat ccatttgatg caagtgcttt
    11461 gaacagagag attgaacaaa tgccgatgaa agtcgctaaa ctttccggtt atctttatca
    11521 aaatatccag gtggatgaaa atggtgcagg aagaataatc cttccgcaag aaattcttga
    11581 taaatatgga atcgatgatt cagagacagc ttcgatcgtt tcacttcctg gagtgattga
    11641 cgaagtgctg gcttggggga tttttgttca gcagccttca gggtattacc gcgtacgttt
    11701 acgttctaag gggccgatca tcaatgaatt ggctaaacgc catcatggag gaggccatcc
    11761 attagcaagt ggtgcgaatg caaaagattt agcagaagta gaggaaatct ataaagagat
    11821 ccaagatata tgccaatcgt ataagagcag aaaaacagaa tgatacttcg ttcgaaaacg
    11881 tgccttttgt agtaaaaatc acaaatgtat cttttcaaga tcttcttttt ccgttacaat
    11941 agtgctcact aaagatttta aaggagaaag aagatgacat taccaaattg cccagaatgt
    12001 ggctcagagt acacatatga agatcgaggc ttgttgattt gcccagaatg cgggaacgaa
    12061 tggaacaaag aagaacaaga aactgtcata gaagacggat tagtggtatt agatgcaaat
    12121 ggaaaccgtc tgttagaggg cgatagcgtg acagtgatta aagacttgaa agtaaaaggt
    12181 gcgtcaggtg ccattaaaca aggaacaaaa gtcaagaaca tccgtttagt agaaggcgat
    12241 cataatatcg attgcaaggt agaaggattt ggaccgatga aactgaaatc ggaatttgtg
    12301 aaaaagaatt aaagaatatt caaggtcttt tcacattctt ttcctactac taatgataaa
    12361 ataaagtaaa cgaaaaggag tgaaacgaaa atgtatggtt atggaatgta tggtttttta
    12421 gatccgacat ttcttttggt cattcttgga ttagttattt ccggaattgc gtcagcgtac
    12481 gtcaatagta ctttccgaaa atacgatcaa gtgagaagca aaaacaatgt gactggtaca
    12541 caagcagcac aatatatatt acaaagtcaa cagataaata atgtcggtgt tcaacaaatt
    12601 gccggagatc tcacagataa ctacaattca ggtaataaga tgttgagttt gtctcaagca
    12661 actgcacagt ctacttcagt tgctgcgata ggtgtagcag ctcatgaatg cggacatgcg
    12721 gtacaagatg cttcgggtta tgtcccattg aagattcgtg cagcacttgt tccagcagct
    12781 aactttggtt cgatgatttc gttccccttg attatggtag gtgtattgct tagctggaac
    12841 tctacgttaa tcaatatcgg gattttcgct ttttctttag cattactttt ccaattggtg
    12901 acattaccag tcgaatttaa cgcgtctcgg cgagcaatcc aaattttgag tgaaggcgga
    12961 ctactgacgg aagaagaagt accgatggca agacacgtat tatttgcagc agcactgact
    13021 tatgttgcag cagcgctttc gacattcctg cagctgttgc gcttgatctt attgttcggc
    13081 ggcaaccgaa gagattaaaa taaagaagtt gtgaaatcag cgctttgatc tgagctaata
    13141 ccgagagtta gctgattgaa taccatttat ttggaataag ggctctttgt caataaggac
    13201 tgatgggttg tacaaaatca aatctgggtc agaatgaacc tcattctggc tcag
//
LOCUS       DANKAZ010000070        13185 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000070 DANKAZ010000000
VERSION     DANKAZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..702
                     /locus_tag="PWY67_001977"
     CDS             <1..702
                     /locus_tag="PWY67_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDL2058237.1"
                     /translation="SNYYEGGNEIVSKMKISEVTLDETDDFTYYKGYKKGWNTLCTYL
                     KIRVPFDDLDFFESHKDLITQTASSIYDKQGDNVLVDTILVPLPENYEVINFSQLKIS
                     DVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEILKKYEITVPRDENLSKLYSRI
                     QQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKREAKLIIG
                     ISSTVTDYISGYLDK"
     gene            769..1263
                     /locus_tag="PWY67_001978"
     CDS             769..1263
                     /locus_tag="PWY67_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2058238.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1319..1957
                     /locus_tag="PWY67_001979"
     CDS             1319..1957
                     /locus_tag="PWY67_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058239.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            2029..2424
                     /locus_tag="PWY67_001980"
     CDS             2029..2424
                     /locus_tag="PWY67_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2058240.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2408..4861
                     /locus_tag="PWY67_001981"
     CDS             2408..4861
                     /locus_tag="PWY67_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2058241.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4858..6885
                     /locus_tag="PWY67_001982"
     CDS             4858..6885
                     /locus_tag="PWY67_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058242.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6882..7904
                     /locus_tag="PWY67_001983"
     CDS             6882..7904
                     /locus_tag="PWY67_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL2058243.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7921..8850
                     /locus_tag="PWY67_001984"
     CDS             7921..8850
                     /locus_tag="PWY67_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2058244.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            9095..9211
                     /locus_tag="PWY67_001985"
     CDS             9095..9211
                     /locus_tag="PWY67_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL2058245.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            9227..10030
                     /locus_tag="PWY67_001986"
     CDS             9227..10030
                     /locus_tag="PWY67_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL2058246.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            10265..10501
                     /locus_tag="PWY67_001987"
     CDS             10265..10501
                     /locus_tag="PWY67_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058247.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            10508..12460
                     /locus_tag="PWY67_001988"
     CDS             10508..12460
                     /locus_tag="PWY67_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2058248.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(12532..13029)
                     /gene="dfrG"
                     /locus_tag="PWY67_001989"
     CDS             complement(12532..13029)
                     /gene="dfrG"
                     /locus_tag="PWY67_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2058249.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         4543 a         2180 c         2545 g         3917 t
ORIGIN      
        1 tcaaattatt atgaaggagg taacgagatt gtttctaaaa tgaaaatatc agaggtaact
       61 ttagatgaga ctgacgattt tacttattat aaaggatata aaaagggctg gaatacgctt
      121 tgcacatatc ttaaaataag agttcctttt gatgatttag acttttttga aagtcataaa
      181 gatttgatta cccagacagc aagctccata tatgataaac aaggtgataa tgtattagtg
      241 gatactattt tagtaccact tccagagaat tacgaagtaa ttaactttag tcagttgaaa
      301 attagtgatg tggtttcaca agctattgaa gacgctgagt cttttatgag taatggtgaa
      361 tatcaacgtg cctttgacag agttcacact gcttttcatg gatatttaat tgaaatacta
      421 aaaaaatatg aaattacagt tcctagagac gaaaatctat ctaaattata tagtaggata
      481 caacaattaa ttgaaaaaga aattcaacct actgaacttg ctgatatagt gaagacaacg
      541 ataaggtcat caaatggaat gattagctct cttaatgaag caagaaatag gcattcactt
      601 gcacacccta atacaaatat tattggaaag agagaagcaa aactaataat aggtatttca
      661 agtacagtta cagattatat tagcggatac cttgataagt agcgtcatta actgattgct
      721 acttttttta tgactatttt tataaaaagg atgtgataag aattggaaat gaaagtctat
      781 gtggcaaatt taggacggta taatgaaggt gaattagtcg gagcatggtt tactccacca
      841 attgatgagg aagaaatggc agaaagaatc ggtttaaatg aggactacga agaatatgca
      901 atacatgatt ttgagcttcc ttttgatgtg gacgaataca cgccgattag tgaaatcaac
      961 cgtctatgtg aagccattca agaaatcgaa ggtacaccta tttataatga actcaaagaa
     1021 attcaaggca tgtggtttag tagcttggaa gaattactgg aaaacaaaga ggatattcat
     1081 tgttatagtg attgtgattc gatggaagat gtcgcccgtt attacgtgga agaaacagga
     1141 cagcttggtg aagtcccttc caatttacaa aattatattg actaccaatc tttgggacgt
     1201 gatatggaaa tagagggtaa ttacctagtt acctctcatg gggtatttga atattgtcag
     1261 taagtttaac aatccgtgtt ataattaaat taataaatag cacggagagg tataaattat
     1321 ggtaaatgta tttgaaaatc ttaaactatt aaaactagac atgaaagata aaggctgggt
     1381 gatagattcc ttttactttc gttacaaaca gcaaaattat attgtacttg ttaagctatt
     1441 tgaaaaagag gaaaaagttc cagaatatgc tctgttaaaa ctagaatttt taaaggaaaa
     1501 cgatttttca gatatgctag ctgtctatgc taattcggta aaattatata cagacacgaa
     1561 aacaataaga gaatattttg gtatcgaata cagtagtaac ttgggagatg ttttatttca
     1621 atttagccaa actcttgctc gatttattcc gacagaagtt agtgagaaga aaaatgaaga
     1681 ccaaaaagaa gccatgtgtt tctcattaag tcaaagtgat tcagaagatc ccagaaaaaa
     1741 atattgtttt tcagttagaa gaaatccttt aaagggaaat ggtgagctgg gaaaacgaag
     1801 tccttttaat gacaataaaa cgagattgta tagacccagc ttatatgaac gattagggtc
     1861 agataccaat ttaagtttca gatattcaat gaatccagat gatgaagaaa ctgatgaagg
     1921 tattattgca aaatggacaa aaaataaaat tgaatagtta aagcattgaa agagcaatca
     1981 cctattttga ttgttctttt tctattttta cggaaaggac gtgaagacat gaaaaaaata
     2041 agaagttaca ccagtatttg gtcagtagaa aaagtgattt atgcgattaa tgatttacaa
     2101 ctccctttcc caattacctt tacgcaaatg acatggttta tcgtttcttt gtttgtcgtc
     2161 atactctttg gcagtttacc accactttct ctgattgacg gagcactact caaatactta
     2221 gggattccca ttgttctcac tgggttcatg tgccaaaaga cctttgacgg aaagaaaccc
     2281 tatggatttc taaagtctat cattacctat gctctacgcc ctaaagtaac ctacgctggc
     2341 aaacaaatac gattaaaaaa agaaacaatg aatgaatcga ttacgattgt aaggagtgaa
     2401 caactagatg gcatatccga ttaaatatat ggaagataat ctcgtattta atcatgatgg
     2461 cgaagtattc gcttattatg aattaattcc ctataattac agttttttaa gttccgatga
     2521 aaaaattcaa gttcatgata atttctgtca attgattgcc caaaatcgtg atggcaagat
     2581 tcatgctctg caactagcaa ccgaatcaag tattcgagcc acacaggaac aatcgaaaaa
     2641 agaaatcact ggacgattaa aagagatagc ttacgaaaaa atagatgaac agaccgaagc
     2701 gttaatttct atgattggtg acaatcaagt agactatcgc ttttttattg gttttaaact
     2761 ctacctcaat gaacaggaag tatccgtaaa aaatgttggg aaagaaattc aaaaagcatt
     2821 tgcggaattc tttcatggtg tgaatcacaa attaatgggc gattttgttt ctatgcctat
     2881 ggcggaaatc gaacgctttt ctaagatgga acagttgtta gaaagtaaat tatccagacg
     2941 ctttcagatt cgtaaattgg agaaagatga ttttggttat attattgaac atctttacgg
     3001 gcaaacaggt gtcccttttg atgactacga ctaccattta ccgaaacgat acgaagataa
     3061 aaaagtgttg gtaaagaaat acgatattct caaacccact cgctgtttaa tcgaagaaaa
     3121 tcaacgctat ttacggattg aacaggaaga taccactact tatgtggcgt attttacaat
     3181 taatagtatt gttggtgaat tggatttccc taatagtgaa attttttatt accagcaaca
     3241 gcagtttact ttccccgttg ataccagtat gaatgttgaa attgtcgcca ataaaaaagc
     3301 cttatctacc gttcgcaata aaaagaaaga actgaaagat ttagatagcc acgcatggga
     3361 aagcgacaac gaaaccagta ctaatgtggt ggacgcttta gaatctgtcg atgaattaga
     3421 aagtaattta gaccaaacca aagaatctat gtacaaacta agctatgtgg tacgggtatc
     3481 agcaaagaat ttggacgagc tgaaacaacg ttgtgatgaa gtaaaagatt tttatgatga
     3541 tctgtctatc aaattagtac gaccttttgg cgatatgcta ggcttgcata atgaatttat
     3601 tccctcaagc aagcgatata tcaatgacta tattcaatat gtcacgtcgg attttttggc
     3661 tggtcttggt ttcggtgcta cacaaatgat tggtgaaaat tctggtattt acgtcggtta
     3721 caatctcgat acaggcagaa atatttatct ccaacctagc cttgcaagtc aaggaattaa
     3781 aggctctgta acaaatgcat tagcgtccgc ttttattggt tcattaggtg gcggaaaatc
     3841 attctctaac aatatgctgg tctattattc agtactcttt ggcggacagg caaccattgt
     3901 tgacccaaaa gctgaacgtg gtgaatggaa agaaacctta cctgatattg cccatgaaat
     3961 caatattgtt aatctcacga gtgagaacga caataaaggg ttacttgacc cttatgtgat
     4021 tttaaaactc ccgaaagatt cagaaagcct tgccattgat attctcacgt tcttaacagg
     4081 tatttccagc cgagatagcg agaagtttcc tgtcctgcgt aaagctattc ggagtgtaac
     4141 tcaaagcaaa caacgagggt tgttattggt gattgatgaa ttgcgaaaag aagatacgcc
     4201 catcagcaat agtattgctg accatatcga aagctttgtc gattatgatt ttgcccatct
     4261 gttattcagt gatggcagtg tcacccaatc cattagcttg gaaaaacaac tcaatattat
     4321 tcaagtggca gatttggtct tacccgattc caatagctct tttgaagaat acactaccat
     4381 ggaattatta agtgtggcaa tgctgattgt cattagtacc tttgctttag actttatcca
     4441 ctctgaccgt tccattttta aaattgttga ccttgatgag gcttggagtt ttttacaagt
     4501 ggcacaagga aaagctctat ctatgcgatt ggttcgagca ggacgtgcca tgaatgcagg
     4561 ggtctatttc gttactcaaa acgcggacga tttactagat gaaaagatga agaataatat
     4621 tggcttgaaa tttgcctttc gttccactga cctagtggaa atcaaaaaga ccttagaatt
     4681 ttttggtgtg gacaaggaag atgaaaacaa tcagaaacgc ttacgtgaat tagaaaatgg
     4741 acaatgttta gtttcagaca tctatggtcg tgtaggggtt atgcagtttc acccgatttt
     4801 tgaagagttg cttcatgcct ttgataccag accgcctgtg cgaaatgagg tggaagaatg
     4861 acacaaaaac aaaagaaatt tcttcactat agttggataa cgctcctttg tgtgggcgtt
     4921 attttacttt tattaggcac gctgggcaat gcagtacaag cgacaggctt agttgatgaa
     4981 accattgata caagcaatga atactccaag tatggtttga atcaatatca gcttgattat
     5041 tatgtggaca atagctgggg ctggcttcca tggaattgga gtgatggcat aggacaatcg
     5101 gtgatgtatg gattgtatgc cattactaat tttatttgga caatttctct ttatctatcg
     5161 aacgccacag gttatttggt gaaagaagcc tatgccttag atttcattag tcaaacatcc
     5221 caagcaattg gcgaaaacat gcagacgatt gcaggcatta caccgaatgg cttttccagt
     5281 tcagggtttt atgtcggttt cctccttctt tttattctca ttgttggaat ctatgttgcc
     5341 tatacaggat tggttaagcg agaaacgacg aaagtgatac gagctgtttt aaactttgtc
     5401 gtggtgttta ttctatcggc ttcctttatt gcgtatgcac ccgattatat tgcgaaaatc
     5461 aatgattttt ccaaagatgt cagtaccgcc agtctgaata ttgggacaaa aattgttctt
     5521 cctaacacgg atagtcaagg gaaagacagt gtggacatga tacgtgacag cttattctct
     5581 gttcaagtaa agcaaccatg gctattgctt caatatggct ccactgatgt agaagcgtta
     5641 ggggaagaac gtgtggaaag tttattgtct accagtccag atacaaacaa tggtgaagat
     5701 agagaaaatg tagtgattga agaaattgaa gatcatgaca atacctattt aaccctaccg
     5761 aagacgattt ccagattagg aacagtcttc tttttgtttg tattcaatat tggtatctcg
     5821 atttttgttt tccttctaac aggaatcatg attttttcgc aagtgctatt tattattttc
     5881 gctatgtttt tgccaatcag tttcctatta agtatgttgc cgagttttga tggcatgggc
     5941 aggcatgcca ttaccaagtt atttaatgtg attatgacaa gggctggtat taccttgatt
     6001 attaccgttg cctttagtat ttccactatg ctttattcgc tgtcagcaag ttctcccttt
     6061 ttcatgatta tgtttttaca aattgtgacc tttgcaggga tttattttaa actgggcgat
     6121 ttaatgagcc tattcacttt acaaagcaac gactctcaaa acatgggcag acaagtgttt
     6181 cgcagacctc gtcaaatgat gaatcgacaa accagaagat ttcaacgcag tatgagtaag
     6241 atttttcgtg gaaaagcagg gtcaacgaag caaaagacaa tgacaacaaa gcagaatcaa
     6301 accaataaag ctaaccatac tcgtcaaaat gaacggacta caccaaagaa agaaacagca
     6361 ctttccaatg tgaaaaagca acaaggcggt atcgggcaac gattaggtga aaagacagcc
     6421 catgtattgg acacgaaaaa tcgtctagtc aataaaagaa agcaggcaaa acaacagata
     6481 caggcgacac caacccatgt caaatatgcc cttcatcaag gaaaagagaa tgtcactcat
     6541 aatgtaacag actttaaaca atctatggca gataccaaag ctcaacgcaa acaacagcgt
     6601 atgaaccagc aggagcaaaa acgaaagaac attgctcaaa agcgtttgga gctggataaa
     6661 gcaaaagaaa aaagacgaaa agaaaaccca tcatctcctg ttcagaataa ccagcagcat
     6721 gttcgcaagc gtcagcaaac cgtcacgcct gttttgtcac aaaacactaa acgtgaaaat
     6781 catcagcgac cattgaaaaa agagcaaaaa caaaagacac caccgaagca attcaatcag
     6841 aggaagctaa ataaaagaac ggataagcga ggacaacaaa catgaagcta aaaataacag
     6901 tgatttgtag tgtggtcttc tctctcttct tttttctctt attattccta gtcattttct
     6961 ttgcagatga tacggacagt ggcgaaaaca acaaggattc ttctattcca caaggcggtg
     7021 tgaccgtttc accagaagtg ttggcacacc gtccgttgat tgaaaaatat ggtaaggaat
     7081 atgggattga agactatgtt tcttatatct tagccattat gcaagtggaa tcgggcggta
     7141 cagcagaaga tgttatgcaa agttccgaat ctttgggttt accgcccaac agtttgagta
     7201 ccgaagagtc catcaaacaa ggggttaaat atttcagtga attattaacc agtgcagaac
     7261 aacaaggagt ggatatagat tctgttatcc aatcttataa ttatggcggt ggctttttaa
     7321 attatgtgag gagtcatggg aaaaaatata cctatgaatt agccgaacag ttttctaaag
     7381 agaagtcggg tggtcaaaaa gcagactatc ccaatcctat agccatacct gtgaatggtg
     7441 gttggcgata taactatgga aatcagtttt atgttcagtt ggtttcacaa tacctaaccg
     7501 acacttcccc aacagaattt gatgatgaaa ccgtgcaagt cattatggac gaagccttga
     7561 aatacgaagg gtttccctat gtctttggag gggcgtcgcc taccacctct tttgattgta
     7621 gtggcttaat acaatgggtg tatgacaaag ctggcatttc tctccctcgt gtcgcccaag
     7681 atcagtatga cgctacgcaa gaaatctcta tggaagaagc ccaagcaggc gaccttatct
     7741 tctttcattc aacctataat gcaggcacat atgtgactca tgtggcgata tatttagagg
     7801 gaaatcgctt ttatcatgca ggcgacccaa tcggttatgg cgacttaagc agtcgttatt
     7861 ggcaagacca tctgattggg gctagacgtg ttattcataa ctaagaaagg aatgaatcaa
     7921 atgattataa aaatcgagcg aaaagaaaag aaggagaaac caaagaaatc taaaaaaccg
     7981 agcaagcaaa gaaaagttcc gatgattaaa gttggcacgc ataagaaatt aacctttgtt
     8041 ttatgggttt tattgattgg tagtgtcgga tttggaatct ataaaaattt tactgccatt
     8101 gacactcata ctgtccgtga aacagaaatc attaaacagc agattgtcga taccaaccaa
     8161 gtagaaagtt ttgtgaaatc ctttgcgaaa gactactttt cgtggcaaca atcccaagaa
     8221 gccattgata aacgaaatga aaaattgacc cattatttga cggaagaatt gcaggtatta
     8281 aatgaagaaa tgattcgtaa ggatattcct actagttcta gtgtgaacga tatacaggtg
     8341 tggcaggttt ctcaagtgaa tgagaacacc tttgaggtac tgttttctgt tgaacaagtt
     8401 attaccgaag acaaagacaa ggaaactata tcgtctagct ttcatgtggt tgtccatata
     8461 gatgaatcag ataatatggt tattatcaaa aatccgacaa tgagtaagaa acctcaaaaa
     8521 tcggactacc aacccaaaca gctagaaagt gaccatacag tagatacaga aacgatggac
     8581 gaaatcacta gctttttaga aacgttcttc cagctctatc ctaccgccac agaaaaagaa
     8641 ttgacttact atgtaagtaa tcatgtgtta cctatgatta acaaggaata tgttttcgag
     8701 gaattggtga atcctatctt taccagaaaa gataaccaag taatcgtgaa tgtcgctgtg
     8761 aaatatttag atcaagaaac aaaggcaacc caaatctcgc aatttgagtt gatattagaa
     8821 aaacaagata attggaagat tgtaaaataa caaatattgg tacatgatta cagatacttt
     8881 gtaatcatgt actctttttg ataaaaaatt ggagattcct ttacaaatat gctcttacgt
     8941 gctattattt aagtgactat ttaaaaggag ttaataaata tgcggcaagg tattcttaaa
     9001 taaactgtca atttgatagc gggaacaaat aattagatgt ccttttttag gagggcttag
     9061 ttttttgtac ccagtttaag aataccttta tcatgtgatt ctaaagtatc cagagaatat
     9121 ctgtatgctt tgtataccta tggttatgca taaaaatccc agtgataaaa gtatttatca
     9181 ctgggatttt tatgcccttt tgggtttttg aatggaggaa aatcacatga aaattattaa
     9241 tattggagtt ttagctcatg ttgatgcagg aaaaactacc ttaacagaaa gcttattata
     9301 taacagtgga gcgattacag aattaggaag cgtggacaaa ggtacaacga ggacggataa
     9361 tacgctttta gaacgtcaga gaggaattac aattcagaca ggaataacct cttttcagtg
     9421 ggaaaatacg aaggtgaaca tcatagacac gccaggacat atggatttct tagcagaagt
     9481 atatcgttca ttatcagttt tagatggggc aattctactg atttctgcaa aagatggcgt
     9541 acaagcacaa actcgtatat tatttcatgc acttaggaaa atggggattc ccacaatctt
     9601 ttttatcaat aagattgacc aaaatggaat tgatttatca acggtttatc aggatattaa
     9661 agagaaactt tctgccgaaa ttgtaatcaa acagaaggta gaactgtatc ctaatatgtg
     9721 tgtgacgaac tttaccgaat ctgaacaatg ggatacggta atagagggaa acgatgacct
     9781 tttagagaaa tatatgtccg gtaaatcatt agaagcattg gaactcgaac aagaggaaag
     9841 cataagattt cagaattgtt ctctgttccc tctttatcat ggaagtgcaa aaagtaatat
     9901 agggattgat aaccttatag aagttattac taataaattt tattcatcaa cacatcgagg
     9961 tccgtctgaa ctttgcggaa atgttttcaa aattgaatat acaaaaaaaa gacaacgtct
    10021 tgcatattaa gtagttcagt tttggagtac aaaaagtgaa aatgtaaacc taaatgagta
    10081 tcaaattgca actgaatttg atactcgttt tttattgtta aatcaacgtt tgttgttaat
    10141 ttacaccaca aaatgtaagt taaaatgtat ataaaaatgc gaacgtgaaa taattattgt
    10201 aggaatgcaa attggtgtgt taaaatggtt acaatttact aagcaaaaag gggtgcattt
    10261 tcctatgcaa tttcttgagc caaaaaataa gaacgctaag tctgtggatt gggaaatatc
    10321 agagcaagta agggtgatag ttaagcaata cgctgaatat gcagagcgta ctgaaagcga
    10381 agcagtggat gaattcttat tgaacatttt agacgataag aagtttattg aatggattgc
    10441 caacaagcga agtaataagc ggattgttga aaaaatgggt atcaaagata gagtgggttg
    10501 atagacaatg accaagctta aaagattagc cacgaaggaa gatgaaattg tagatgttaa
    10561 aattccgatt tcaaacgaag aattaaaaga tagggctaaa caatatgatt tattaactcc
    10621 aaaacgattt gcaacaaggt ataacaaaat gttgttcttg ccaacgtctt ttaaatggaa
    10681 cggttctgaa tatcccattc aatataatta ctgcatcaat ccgttttgtt gtaattttgg
    10741 gaaagaacag cacaaattca aagatgtaaa agggaaacca agtcgatata aaatgactgg
    10801 tagttccaaa gataaaggtc ataaaggtat gtactgcaat gacaatccta ttggcagagg
    10861 tgtatcgcaa aactgtacag taaccccttt atctaattgg tctgttgttg aagaaattaa
    10921 acgccttata gaaatcaata gcatccaaga tgttgagcca gattatcaat ttcataaaga
    10981 aggctgctcg gaagaagaat caaccccatt caatgagccg aagcaatttt acaaaagggg
    11041 gaaaagtagg ggcaaatctc aacgctatca atgtaaggcg tgtaagaagt tcactaatgt
    11101 tctaccaaaa agagaagaaa ccactactta tcaccaacaa aagaacacca ttcttccaat
    11161 gttcgctaag atggttgttg gaagggtttc agtcagtcgt acttgtgata ttttaggaat
    11221 tggagttgga acctactatc ataagctgga gtggctgtat aggcgttgtt tggagttttt
    11281 agaacgatat gagacacaac cccttcaaac aaagaaattt aacgagatgt ggcttaatac
    11341 ggacaagatg cactactacc taaataacgt ccgtaagaaa ggacaaggtt caaagaaata
    11401 tacggggttt gaagacctta atatgcaaac gtatatagtt gtatctgctg aggtcttatc
    11461 aagatatgtg ttccgttctg atgttgccta tgattggaat atttcaatgg atgaattaaa
    11521 tgaagataca agaaaattca aagaagacca tctcaatact tttagtcgta aaaatgatag
    11581 gttagattgg tcttattatc cacaagaacc atccgctaac gatagcgaaa ataggaatgc
    11641 ttatcttcac gaattaggga aaattactaa taggagtaga tttgtcgatg gtttaaatgt
    11701 tgatgctcct tatactacaa ctgctcacta ttggttaatt aaacaaatgg ttaatgccga
    11761 cgaatggcga atgattagtg atgatgactt ctctattaga aacgcctttt atagagtatt
    11821 cacgaaagaa ttaaggttga gcgatgcaca tcattttatt tgccaagtta acaagacgaa
    11881 aagcagaaaa caatgcttaa aagaattcgg acaagcaaaa gcagaactgc tcgattgggg
    11941 agatatcaga ggattcaaaa caaagtttct acggactttg gcatctcatt atctaactga
    12001 attactaaca agtcatcaat tccacgaaga ggctattagt aaagatggag aaagatatcg
    12061 caaatatgcc gataacccta tcaaacatcc attagccact aaggataaag gattttattc
    12121 agttgattgt agaacggatt tatctgctct ggaaccaaac gaaatcgcaa agatgctatt
    12181 aaatgttaat gaccattcga ccaatagctt tattcaacag ataagacgtt atatatcatc
    12241 tcttgaaaga ccattaacaa cagctcgtgg tgataagaag agctatattt acgctaactt
    12301 caatccgaag tatgctcaat ttgcgattac aattctaaga acttactaca acttttgtcg
    12361 cccttttaaa tcagcggata aaaaggtttt gacacctgct caaagattag ggattacaga
    12421 taaacaattt gattggaaag atataattta tttcaaatga caaaaagagc ttggagtaat
    12481 cccaagctct tgtgctttat ctttactgtt cttcttcaat taacgcaccc gttaactcaa
    12541 taagtttttt ctttcatata catgaaaata gtagttatac ggatttttat cattctttat
    12601 ccctttttgg gcaaatacct cattccattc ctcataattc acttctggaa aaaaagtatc
    12661 tccttcgaat tcatgatgta tttttgtgat gtacattttc tcaacataag ggaaaaacaa
    12721 attataaatc tgttctcctc cgaaaataaa aatttcttct tcgtttttac ataactcaaa
    12781 aacatcttct attgaatgaa caatttcaca accattaaag gtaaacccct tatctctcgt
    12841 cagaataata tttcttctgt caggtaaggc tcttccgatt gattcaaggt tcttcctacc
    12901 taatattatc ggatgtccct ttgtagtatt tttaacatat tcccagtcct tgggaatcct
    12961 ccaaggaatg tcattctctt tgccaatcac tctattctta tccatcgcag caatcaaaga
    13021 aactttcatt tataacacag cctttttaac taatttacca tctaatttct tatttctatc
    13081 aaaaaacctt ttctcattat cctgcccgtt agttgaataa gcaatttttt ctataagtta
    13141 tattatcata ataccacaaa atcacaaatt ttcattcaat tatca
//
LOCUS       DANKAZ010000071        13165 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000071 DANKAZ010000000
VERSION     DANKAZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            12..84
                     /locus_tag="PWY67_001990"
     tRNA            12..84
                     /locus_tag="PWY67_001990"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="PWY67_001991"
     tRNA            88..160
                     /locus_tag="PWY67_001991"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="PWY67_001992"
     tRNA            168..249
                     /locus_tag="PWY67_001992"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="PWY67_001993"
     tRNA            267..339
                     /locus_tag="PWY67_001993"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="PWY67_001994"
     tRNA            351..422
                     /locus_tag="PWY67_001994"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="PWY67_001995"
     tRNA            439..524
                     /locus_tag="PWY67_001995"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="PWY67_001996"
     tRNA            535..608
                     /locus_tag="PWY67_001996"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="PWY67_001997"
     tRNA            618..691
                     /locus_tag="PWY67_001997"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="PWY67_001998"
     tRNA            711..783
                     /locus_tag="PWY67_001998"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="PWY67_001999"
     tRNA            811..884
                     /locus_tag="PWY67_001999"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="PWY67_002000"
     tRNA            898..971
                     /locus_tag="PWY67_002000"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="PWY67_002001"
     tRNA            995..1084
                     /locus_tag="PWY67_002001"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="PWY67_002002"
     tRNA            1097..1170
                     /locus_tag="PWY67_002002"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="PWY67_002003"
     tRNA            1189..1264
                     /locus_tag="PWY67_002003"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="PWY67_002004"
     tRNA            1266..1336
                     /locus_tag="PWY67_002004"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="PWY67_002005"
     tRNA            1350..1423
                     /locus_tag="PWY67_002005"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="PWY67_002006"
     tRNA            1448..1535
                     /locus_tag="PWY67_002006"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="PWY67_002007"
     tRNA            1557..1628
                     /locus_tag="PWY67_002007"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="PWY67_002008"
     tRNA            1651..1740
                     /locus_tag="PWY67_002008"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="PWY67_002009"
     tRNA            1753..1826
                     /locus_tag="PWY67_002009"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="PWY67_002010"
     tRNA            1845..1917
                     /locus_tag="PWY67_002010"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="PWY67_002011"
     tRNA            1921..1993
                     /locus_tag="PWY67_002011"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="PWY67_002012"
     tRNA            1998..2068
                     /locus_tag="PWY67_002012"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="PWY67_002013"
     tRNA            2081..2154
                     /locus_tag="PWY67_002013"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="PWY67_002014"
     tRNA            2166..2237
                     /locus_tag="PWY67_002014"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="PWY67_002015"
     CDS             2503..3696
                     /locus_tag="PWY67_002015"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL2058250.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="PWY67_002016"
     CDS             3826..5298
                     /locus_tag="PWY67_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2058251.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="PWY67_002017"
     CDS             5381..6169
                     /locus_tag="PWY67_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL2058252.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="PWY67_002018"
     CDS             complement(6236..6904)
                     /locus_tag="PWY67_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL2058253.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="PWY67_002019"
     CDS             7019..8731
                     /locus_tag="PWY67_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2058254.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="PWY67_002020"
     CDS             8734..10503
                     /locus_tag="PWY67_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2058255.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="PWY67_002021"
     CDS             complement(10708..11274)
                     /locus_tag="PWY67_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2058256.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="PWY67_002022"
     CDS             complement(11274..12230)
                     /locus_tag="PWY67_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL2058257.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="PWY67_002023"
     CDS             complement(12230..12886)
                     /locus_tag="PWY67_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2058258.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT         3838 a         2462 c         2932 g         3933 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaa
//
LOCUS       DANKAZ010000072        13163 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000072 DANKAZ010000000
VERSION     DANKAZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            5..736
                     /locus_tag="PWY67_002024"
     CDS             5..736
                     /locus_tag="PWY67_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2058259.1"
                     /translation="MIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKSTML
                     RCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKLAP
                     IKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEMLLF
                     DEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLEDS
                     RNVRDFFENPNEVRAQQFISKVINH"
     gene            750..1565
                     /locus_tag="PWY67_002025"
     CDS             750..1565
                     /locus_tag="PWY67_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2058260.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1578..2225
                     /locus_tag="PWY67_002026"
     CDS             1578..2225
                     /locus_tag="PWY67_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2058261.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2241..2897
                     /locus_tag="PWY67_002027"
     CDS             2241..2897
                     /locus_tag="PWY67_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2058262.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3059..3880
                     /gene="racE"
                     /locus_tag="PWY67_002028"
     CDS             3059..3880
                     /gene="racE"
                     /locus_tag="PWY67_002028"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL2058263.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3883..5238
                     /gene="rph"
                     /locus_tag="PWY67_002029"
     CDS             3883..5238
                     /gene="rph"
                     /locus_tag="PWY67_002029"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL2058264.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5239..5757
                     /locus_tag="PWY67_002030"
     CDS             5239..5757
                     /locus_tag="PWY67_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2058265.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5864..6364
                     /locus_tag="PWY67_002031"
     CDS             5864..6364
                     /locus_tag="PWY67_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2058266.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6644..7564)
                     /locus_tag="PWY67_002032"
     CDS             complement(6644..7564)
                     /locus_tag="PWY67_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL2058267.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7729..8742
                     /locus_tag="PWY67_002033"
     CDS             7729..8742
                     /locus_tag="PWY67_002033"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL2058268.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8852..9607)
                     /gene="fabI"
                     /locus_tag="PWY67_002034"
     CDS             complement(8852..9607)
                     /gene="fabI"
                     /locus_tag="PWY67_002034"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL2058269.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9827..10267
                     /gene="fabZ"
                     /locus_tag="PWY67_002035"
     CDS             9827..10267
                     /gene="fabZ"
                     /locus_tag="PWY67_002035"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2058270.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10382..11143)
                     /locus_tag="PWY67_002036"
     CDS             complement(10382..11143)
                     /locus_tag="PWY67_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2058271.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11332..12426
                     /locus_tag="PWY67_002037"
     CDS             11332..12426
                     /locus_tag="PWY67_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2058272.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(12512..>13163)
                     /locus_tag="PWY67_002038"
     CDS             complement(12512..>13163)
                     /locus_tag="PWY67_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDL2058273.1"
                     /translation="GMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIH
                     DRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETAL
                     NQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGIL
                     RRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         4223 a         2283 c         2762 g         3895 t
ORIGIN      
        1 ggcaatgatt aaatttgatc acgtggataa atattatggc aagttccacg cattaaaaaa
       61 tatcaatctc gaattcgaga aaggagaagt tgtcgttgtc attggtcctt ctggttcagg
      121 aaagagcacg atgcttcgct gtatcaatgg attagagacg atctcttcag gtaagctgct
      181 gattaatgat actgacttgc atgataaaaa gacaaaactg acagaagtcc gaaaaaacat
      241 cggcatggtt ttccaacatt tcaatttgta cccaaataaa actgttctgg aaaacatcaa
      301 attagcacca atcaaagtgt tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa
      361 atttttgaaa actgtcaata tgttagataa gaaagattct tatccttcca tgttatccgg
      421 gggacaacag caacgggtgg caatcgccag aggtttagcg atgaatcctg agatgctgct
      481 gtttgatgaa cctacttctg ctttggatcc ggaaatgatt ggtgatgtat tagacgttat
      541 gaaaaaattg gctcatgacg gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc
      601 caaagaagtg gcggatcgtg ttatcttcat ggctgatgga gaagtattgg aagacagcag
      661 aaatgtgaga gactttttcg aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa
      721 agttatcaat cattgatagg aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt
      781 tccttttagg cattgctttt cttgcactag ccggatgtaa aggaaatggg ttggcaaatg
      841 aagatatttt agagcgaagc aaatctacca atgaaatcat ctggggtgtc aagtatgata
      901 ctcgattatt tggaatgatg gatattgaga gcagaactgt acaaggattt gatgtagata
      961 ttgcaaaagc aatcactaaa aagatccttg gtgacaatgg aaaaacagaa tttgttgaag
     1021 tcacttctaa aactcggatt ccattattga aaaacggaaa tatcgatgca attattgcaa
     1081 cgatgacgat cacagatgaa cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg
     1141 ctggccaagc tttgttggta aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg
     1201 cttctacaac ggttctagca gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg
     1261 caccagatgc aaaaatttta gaattagaaa actatgcaga agcatttact gctttacaat
     1321 ctggtcaggg agacgcgatg actacggata atgcgatttt gttagggatt gcagatgaaa
     1381 atccagagta tgagttagtt ggtggaacgt ttactaatga gccttacggg attgcgatca
     1441 acaaagggca agaaaacttc ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag
     1501 atggcacata cgataaaatc tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag
     1561 agtaaggggg agaggcaatg agcgaattga tacaaactta tggaccaatc tttgtcgacg
     1621 ggttcaagat cacattgttt tcgagtgtct tagcacttgt gtttagtttg attatcggaa
     1681 cgttaatggc aatcttccaa ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat
     1741 atgtagaatt ttttagaaac attccattat taatcatcgt gatgtttttt tatgttgtcg
     1801 taccgattta ttggtttaat ctagatggat tccaagcagg tacgattgga ttaacgatct
     1861 atacatctgc ttttattgct gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc
     1921 aaacagaagc aggactatct tctggtttta cctattcaga aatcatgcgt tatattgttt
     1981 taccgcaagc attcaaaatc gttatcccgc cattaggcaa ccaatttatc aacctcgtaa
     2041 aaaactcatc tattttagca atcgtagctg gtttagattt gatgtatcaa ggagacttga
     2101 tcgctagtga aacattcaat accttcgata cgtatatcat cgttgggtta ttttacttag
     2161 ttatcacgct accgttatct tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt
     2221 catagaagga ggggaaatac atggatttta gcggcgcttt ttcatggatg aatatccgtt
     2281 ttttattgga aggattaaag gttactgttg aagtttcatt gttttcgatc atttttagtt
     2341 tcttgatcgg tggtctgaca ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa
     2401 tccttggtgt agtgattgat attatacgga atcttccatt attattgatc attttcttca
     2461 cttactttgc cttacctcaa atcggcattc agatgaacat cttctggtcc gctgtcgcag
     2521 cattaacgat ttttgagtca gctatgttgt cggaaatttt ccgagctgga ctcaatgctg
     2581 ttccaaaagg acagatggaa gcaggattat caacgggact gacttatgta gaatcgatgc
     2641 gaacgatcgt gttaccgcaa gcatttaaat caatgatccc ggcaattgtg agccaattga
     2701 tttcactgat caaagatact tcattagcag taatcatttc tttacctgaa ctttctcatc
     2761 aggcacgtat tatttatgga cagaacacca attatgtgct tccgatgttc atcatcatga
     2821 cacttatgta cttcgtggta tgttacgcgc tttctctgct ttccggttac ttggaaaaga
     2881 gacggtacag ttattaatag atgattcttt tagcggttgt ggcaaactaa agtcacaacc
     2941 gcttttcctc tttccattcg aaaaaaagag atttcgattg ttttaagcaa gtaagagcac
     3001 ttgaaaattc acgctaaaaa gtacacaata gggaggatag aatttattga aaaaaaggat
     3061 gatacgattg acagataatc gccctatcgg atttattgat tcaggtgtcg gcggcttgac
     3121 tgtagtaaaa gaagccctga aacaattacc gaatgaaaat attttatttg taggagacac
     3181 agcacgctgc ccatatggcc ctagacccgc ggaacaggta atccagtata cttgggaaat
     3241 gacggattat ctggtggagc aaggaatcaa gatgctggtg atcgcctgca ataccgcaac
     3301 tgcggtggct ttagaagaaa tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct
     3361 tcccggtact agagcggcag taaaaaaaac acaaaataaa caagttggca ttatcggtac
     3421 gattggtacg gtaaaaagtc aagcttatga aaaagcactg aaagagaaag taccagaatt
     3481 gactgtgaca agtcttgctt gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca
     3541 ttcatcggtg gcgaaaaaaa ttgtggcaga aacattagct cctttaacca ctaaaaaaat
     3601 cgatacattg attttgggat gcacccatta tccattatta cgccccatca ttcaaaatgt
     3661 aatgggagaa aatgttcaac tgatcgattc tggagcagaa acagtaggtg aagtatctat
     3721 gctgttagat tatttcaatt tgagcaattc accgcaaaat ggtcggacat tatgccagtt
     3781 ttatacaact ggctctgcca aacttttcga ggaaatagct gaagactggc ttggaatcgg
     3841 acacttaaat gtagaacata tcgaattggg aggaaaataa ggatgcgtca tgacgggaga
     3901 aaagcccaag agcttcgcaa actggagctt caaacaaatg ttttcaaata tccagaagga
     3961 tcggtagtga tccgttttgg tgatacaaca gttatttgtt ctgcaacaat agaaaattct
     4021 gttccccctt ttttgagaga aacaggaaca ggatgggtga cagcagaata tagtatgctg
     4081 cctcgagcaa cgagtacgag aaatcgtaga gaaagcagca aagggaaact ttctggtaga
     4141 acaatggaaa tccagcgtct tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa
     4201 ttaggagaac gtagtatcat cgtagactgc gatgtgatcc aagcggatgg cggtactaga
     4261 acagcaagta tcactggggc atttgtggca ttgaaattag ccattgaaaa acttttaaga
     4321 gaaaaagaat tgtcagaaga tccaataaaa gaacatcttg ctgcagttag cgtaggaatc
     4381 ttaccagatg ggacttgcgt cacagatttg gattaccaag aagattccgc agcacttgta
     4441 gacatgaatc ttgtaatgac agaatctgga aaattcgtgg agattcaagg aactggagaa
     4501 gaagcaacgt ttgatggtga acagttgaat gaaatgctct tttttggaaa aaacgcgatt
     4561 gaagatttga tcaaagagca aaaacatgct ttgctaacag agtttgctca aaatgatgag
     4621 cggatagaag aaacaaaaac aatcatcatt gcgacaagaa atccaggaaa agcagaagag
     4681 tttcgaaata tgttcaaaga agcgggatat catgtaaaaa cattacttga ctatcctgaa
     4741 ttgccagatg tggaagaaac tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa
     4801 acgattgcac aattgcttga tcagccagtc ttagcagatg attcaggttt gaaagtggat
     4861 gctttaggcg gtatgccagg aatctattct gcacgttttg caggggaaca aaaaagtgat
     4921 gcggggaata acgccaaatt attatatgaa ttgacagatg tgccggatga aaaaagaaca
     4981 gcacaattcc attgtacgtt agtattcgcg gcacccaaaa aagacagcct tgtagtagaa
     5041 gcggaatggc ccggtcgagt agcccgtata ccatctggcg aaaatggctt tgggtatgat
     5101 cctttattta tcccagaagg taagaaacaa acagcagcag aattatcgag cgaagaaaaa
     5161 aacaagatca gtcatcgggc tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg
     5221 ctggaaggag agagatagat gcggtattta gtagtaagtg ataatcatgg agacagagat
     5281 atcctcgttg acttagtaga tcgttatcgt tctgaagtgg atatcttttt ccattgtggt
     5341 gattcggaat tagaagcttc tgacccatta tgggatacat tccaagtggt tcaaggtaat
     5401 tgtgattatg gaccaggatt cgaacagaaa aaagtgatcc aaactggtca ggatacagta
     5461 ttcatgactc acggtcattt atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa
     5521 gctcgagctg caactgccga tatggtgttt tttggtcata cccatcaaat aggctgtgag
     5581 atggatcaga aaatcctgtt tttgaatcct ggtagcattt ctcagcctag aggcccgatc
     5641 caaatcccat catatgctct tatcgaaagt ttagatgacc aaatttctgt tcaatactat
     5701 aaccgtgccc atcaggcagt tgaagagttg gccttttctt tttcgagaga gcgctgaata
     5761 aaaaaactct aatttttaat aaaaataagc tttattttat gaattttgca tccggtttcg
     5821 gtaaaatagt agtgttgaat gaaatagaaa tggaggccat cagatgattg gacctatagt
     5881 aagagaaatg ctgctggaaa atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa
     5941 tgttatgtat catcatccat tgtcccatgg tttacttgtg ttatcgaagg tcggttattc
     6001 caaaattcct gtattaggaa aagatgatca ctttgtagga ctagtaagtt tatcagatgt
     6061 ggtcaataaa atgattgact tgcaaacaat cagcatggac ccgttagaag gactaacggt
     6121 tgctgatgtg acagaaacag atgttccgac aattgatgaa aattgggaac tagaagaagt
     6181 actccatttg ttagtcgatt cgcctttttt accggttgtc acagaagatc ggattttcaa
     6241 aggaatcatt acaagaaaag agctgcttaa agccgtaaac ttcatggtgc atgaattaga
     6301 acgtcgtaat atattgtctc cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag
     6361 ctaaagaacg agaaactaaa agataatcaa aaaataagta gaaaaaacag cgtaccagtt
     6421 ttgactttat cttgaaaaag aggctgggac aaaagcgttt aactccaaga aataagaagg
     6481 aattcacgaa aattgctttt caaatttttg tgaatttcag cttatttccg aaggagttgc
     6541 ttctgcttcc gccgtttata cgtgtttaga gcgtgggaca aaagtgttct ttacttttgt
     6601 cccacgctct ttttagtgag attcgttgca tcgtttgtcc aaattatttt ccgacagcaa
     6661 ttggactgtt cggaatcgtg aaaccagaag ctgtcaattc tttgacatag gagctcaaga
     6721 actcttcttt taaggcatat tgttttccgt tcaatgcgta gcagaccgta cgaatcgcaa
     6781 aattgctgtt tccaagatcc actaatccaa agatagaagg ttcagtttgg agctcttctt
     6841 ggtatttttc agctaatgta cgatttactc gatcaatgat ctcatagatt ttgtcatatc
     6901 cttcttcagg aacaatccga atatctaaaa gtacttgcat atttgcacga gagagattgc
     6961 tgatcgtggt gatgtttcga ttcgggatga aatggacagt accatccgtt gctttcagct
     7021 gaagggtacg gataccaaca gatacgacgg ttccttcaat cgttaagttt gctagcttga
     7081 tgtagtcccc aacatccatc tgttgttcca tgataataaa aaatccggta ataacatcac
     7141 tcatgaaccc ttgggcacca agaccgattg ccacacctgc aattccggca ccagccaaaa
     7201 gtgatccgat cggcacaccg atagcagata aaattgcata gataaaaaag aaaccaaggg
     7261 tataatgaaa aatagtattt aaaagactat gtaaagtctt taagcggctc tcgtttagaa
     7321 tggctttttt agaatactga ttaaaagact tatcaatcag atattttccc acacggtgaa
     7381 gaatccaaaa aaggacgatc aaaaaaatca gatataaact tttttggata aaggcagcca
     7441 aaatggcgtc ccaatcaatc ccgtcccaaa accgctgaaa cgcatttact ttgctgacag
     7501 cttgatctgt caatgtctcg ccagaagagt ctgtcgtacc ggcagttgct aataatgaaa
     7561 acataaaatc ctcttttcta aaatttgatt ctttctgtat tgtaccaaga agttttcaaa
     7621 aaaggaaata gcaaaaataa ctataatgaa ggaaaaaagc tgaaaataaa atgacaaata
     7681 atatggataa tgataaaatg tggagaaaat aacgaaggtg gtaagtatat gcaaatcgat
     7741 tggaacaatc taggtttttc ttatatcaaa actccttggc gttttattgc caaatggaaa
     7801 gacggacaat gggaagaagg agaattgaca gaagataatt acctgacgat tcatgaaggc
     7861 tctccagcgc ttcattatgg tcagcaatgt tttgaaggtc taaaagctta tcgtcgaaaa
     7921 gacggcaagg tcaatctctt tcgacctgaa caaaatagtc gacggatgaa tcaaagtgct
     7981 agacgattgc tgatgccaat ggtccctgaa aagatgttcg ttgatgctgt caagcaagtg
     8041 gtaaaagcaa atgaagattt tgtgccgccc tatggaacag gcgctacatt atacttgcgc
     8101 cctttactga ttggggtcgg tgaaaacatt ggtgtccatc cagctccaga atatttattt
     8161 gtcgtcttct gtacgccagt aggagcgtat ttcaaaggtg gactaaaacc aacgaatttt
     8221 attgtatctg attacgatcg tgctgctcct aaggggactg gtgcagcaaa agtcggcgga
     8281 aattacgcag cgagtttact tccaggaaca gaagcaaaag aactggatta ctcagattgt
     8341 gtttatcttg atccagccac acatacgaaa atcgaagaag taggtgcagc aaatttcttt
     8401 ggaattacaa aagacggaaa attcatcact ccgcaatcgc cctctatctt accaagtatc
     8461 acgaaatatt ccttgttgca gttagctgaa gaacgactag gactgcaagc aattgaagga
     8521 gatattttca ttgatgatct agatcaattt gcagaagctg gtgcatgtgg tacagcggct
     8581 gttatttctc caatcggtgg gatttatcac aataacaatc ttcatgtatt ttatagtgaa
     8641 acggaagtag gaccagtaac aaaacggttg tatgatgaat tgacaggtat tcaatttgga
     8701 gacatagaag caccagaagg ctggattgaa gtcgtggaat aatttcaaag taatgagttg
     8761 aaaaaaacgt aaaaagaaaa aatcaggaag agactttaaa aagttttttc ctgattttta
     8821 tatgttttac tttaaagaat gcaacaaaag tttatgaaag atggacacct ttgtcaacgt
     8881 agataatatc accgatgatt ccgctggaaa gtgggctgat taagaaagca caagcattac
     8941 cgacttcttc gatcgtcaca ccttgctgat caggcgtacg agattcagat aactggatca
     9001 gtttttgata gtctttaaca cctgtgacag ctaatgtttt gattgcccca gctgagattc
     9061 cattgacccg aattccttta ggcgagaact cagctgccaa ataacggata gcagcttcaa
     9121 gagaagcttt cgcaataccc atcatgttat aattcgggat tgcgcgttct gaacctagat
     9181 aagtcatgct aacgatcccg ctgtttggtg caagaaacgg ttgtgcgtat tttgatacag
     9241 caattaaaga gtagctgctg atatcttgag ctaattgata gccgtctctg gaaatatcag
     9301 acacatttcc ggacaattct tctttgttgg cgtaagcaac tgcatggaca aggccgtgga
     9361 tctccccagc atattcaccg atggcattca ttgctttttc gatgctttcg tcgctagaaa
     9421 catcacattc tacagtgaat gcttcttcgc cgaccaattt gactaatgat tttttcattc
     9481 gttcattttg gtaagtatag ataatttctg ccccttgttt caacattgct tcagcacaac
     9541 cccaagcaat gctttttttg ttagcgacgc ccataatgac gatttttttt ccttctagaa
     9601 atgacataat gactctcctt tgtttctgat aaaaatttga tagtcgtaaa aaaatagaga
     9661 ataatagcta aaataacttc ttgatttctt aattattagt caaaatgttt tgattgtcaa
     9721 actatttttt cttaaattaa aaaaagattg ctaaatattt aggaatatga tactttgata
     9781 gtcaaagtaa tgaaaatggc aattgcctga aagaaggagt ttaattatgt cttacgtatt
     9841 atctgctact gaagtaatgg aattgattcc taatcgttat cctatttgtt atattgatta
     9901 tgtagatact ttagaaccag gaaaaaagat cgttgcgaca aaaaatgtaa caatcaatga
     9961 agattttttc caaggtcact ttcctgacaa tccagtgatg ccgggtgcgt tgattctaga
    10021 aacactggca caagcaggtt cgatacttat cctaaaatca gaagagtttc taggaaaaag
    10081 agcttatatc ggtgggatca ataaggccaa attccgtcaa aaagttgttc caggagatgt
    10141 gctgaaatgt cattttgaaa tcaccaaaat gaagggatca gtcggtacag cgttatctgc
    10201 agcatatgta gatgataaaa aagtctgtga gtgcgagttc acgttcatcg tcaaccaaaa
    10261 tgcttgacta taaaacctga tatagcaaaa caaaaggaag cttacttgct ttttatgtaa
    10321 aaagaagacg cctgaatcct agcatacgtt gtcatctatg caggattcag acgttatttt
    10381 tttatttttg ttttgcttct tgcagtatat tgattttatc gatttttact tcttcttttg
    10441 gtttgtctga actatctgtt tcgatatttg caatagcatc cacgacatcc attccttcaa
    10501 tcacttggcc gaagacagta taatggccat ctagtgaagg ataaccgccg tttttataag
    10561 cgtcgatgat tgcttgagga tagtcgtctt ttaaaagacc atcatgcata tcatctgtat
    10621 tttgaacgat aaagaactgg ctgccgttac tgttaggatc ttgtgagcga gccatagata
    10681 acgcacctct tatattataa aggtggttgt tgatctctgt ttcaaatcct tctccccaaa
    10741 tgctttcccc tccagtgcca tcgcctttag gatcgccacc ttggatcatg aaatctttga
    10801 tgacgcgatg gaaagtcagt ccgttgtagt aaccgtcttt tgcatgtgtc ataaagtttt
    10861 caaccgcttt tggtgcgtgt tcgggaaata atttgatttt gattgtgcct ttattcgtca
    10921 ccatttcgac cagatcttca ttttcggcga catctttcgt caattgtggt aattccaatg
    10981 cgttcaaatc gacgctactg gcagtcgtgc tttccgcagt agatgtactt tttgaacttt
    11041 ctgtagacga actagaagaa gtcgtgttgt ttgcagaaca accagcagca aacagcaatg
    11101 ctgctgctaa actaatactt aaaataactt tatttttctt cataactgtt cctttccaat
    11161 ttactatttt gttatcttta atgataacaa aatagaggga aaatacctac ttaaattatt
    11221 ggaatttatc atttatttta aaagaaaaag aaaaggtgta gaccatttac tttgtgtttg
    11281 ctataatgta agtggataca aaaacgaaaa gatgcaggag gaaatggtaa tatgggaaaa
    11341 ttaggaatct cgatttatcc agaacggtca acatttgaaa aggacaaagc gtacttggat
    11401 ttagctcata agtatgggta taaacgggtt tttaccagtt tgctgcagat caatgatgat
    11461 aaagaaaaag ttctttcaga atttaaagaa gtagttgatt acgcgaatag cctaggcatg
    11521 gaagtaatgg ttgatatcaa tccagcgtta tttgaacagt tggagatttc ttacgatgac
    11581 ttatcttttt tccataagat gggtgcctac ggggtccgct tagatatcgg attcacagga
    11641 gcagaagaag caaaaatgac gcgcaatcct tttggtatca aaatcgagat caatatgagt
    11701 tcgggcacga attacgttga caacatcatg agttattcgc caaacacgga taatttatta
    11761 ggttctcata atttttatcc tcatcgatac tctggtttag gatacgaaca ctttgttttt
    11821 tgttcagaaa aattcagaaa atataatttg aatacgatgg cattcgtcaa ttcgcaatct
    11881 gctgagtttg gcccttggcc tacgcaagat ggcttgtgca cgctggaaga ccaccgagat
    11941 cttgaaattg cgacacaagt gaagcattta atccttacag gattgattga tgatatttct
    12001 attggaaatg cgtacgcaag tgaagaagaa ttgaaagaaa tggcagaagc atttaacgcg
    12061 gattatccaa cattgaaagt agatacggaa gaaggtatta cagaaaacga acgaatctgc
    12121 ttgtttgata accttcatag ttatcgaggg gatcgctcag aatatatcct tcgttcaacg
    12181 atgactcgtg tatactacaa agataaagat ttcccgcctc ataatacgcg agatatgcat
    12241 catggggatg tactgattga caatgaaggg tacggtcaat acaaaggaga aacacaaatc
    12301 gccttgaagg atatgaaaaa cgacggacgt gtcaatgttg taggaagaat ctccgatgac
    12361 gaattattcc tccttgactt tttgaaacca tggtcaagct ttaaattaat tgaaaataaa
    12421 aaatagataa ttagttattg cagaaataat ccgctagaaa tagccagtta tttctgcttt
    12481 tttattttgt ttttttatag gttatgattt gtcaaattaa gctagaataa aatgcttctt
    12541 gtacatagct caaaaatatt tcgattggtg ttctgtaatt caatgatttt cttggaatat
    12601 gattacgttg attgctgaca ctggaaataa atgtctgatt cacttttcta aaatccattg
    12661 atttcggcag tccattacga cgcagaatcc cgttagaatt ctcgtttaat gggcgttgag
    12721 aaggtgttcc agggtccgca aaatatatat caatatcatg ttggttacta atggctttcc
    12781 agttagaaaa ttcttttcca cagtcaaacg taatagattt aaagaagttt ttagggaagc
    12841 gagaaaacca ttgattaagg gcagtttcaa tatctaatgc cttacggccg ttgggtttaa
    12901 tcgtgataat gactttagat aatctttcaa ctaaagtaat gacggcactt ttatgatgaa
    12961 tgccaacgat cgtatcacct tcaaggtgac caaactcaga attgaaatca ggataattat
    13021 caggtcgatc atggattgag cgctgatact gttgttttcc ccgtttttcc tgatggccat
    13081 tgggttttct tttacctttc atcggtagtg tgtcaatatc aaatattcct ttagaaaata
    13141 aacgataaag tgttctcata ccg
//
LOCUS       DANKAZ010000073        13093 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000073 DANKAZ010000000
VERSION     DANKAZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            337..543
                     /locus_tag="PWY67_002039"
     CDS             337..543
                     /locus_tag="PWY67_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL2058274.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(745..1746)
                     /locus_tag="PWY67_002040"
     CDS             complement(745..1746)
                     /locus_tag="PWY67_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2058275.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1751..3664)
                     /locus_tag="PWY67_002041"
     CDS             complement(1751..3664)
                     /locus_tag="PWY67_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2058276.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3832..4338)
                     /locus_tag="PWY67_002042"
     CDS             complement(3832..4338)
                     /locus_tag="PWY67_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2058277.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4498..4938
                     /locus_tag="PWY67_002043"
     CDS             4498..4938
                     /locus_tag="PWY67_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2058278.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5097..6437
                     /locus_tag="PWY67_002044"
     CDS             5097..6437
                     /locus_tag="PWY67_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2058279.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6506..7663)
                     /locus_tag="PWY67_002045"
     CDS             complement(6506..7663)
                     /locus_tag="PWY67_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2058280.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7666..8022)
                     /locus_tag="PWY67_002046"
     CDS             complement(7666..8022)
                     /locus_tag="PWY67_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058281.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8320..9294)
                     /gene="sgrA"
                     /locus_tag="PWY67_002047"
     CDS             complement(8320..9294)
                     /gene="sgrA"
                     /locus_tag="PWY67_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL2058282.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9490..10329)
                     /locus_tag="PWY67_002048"
     CDS             complement(9490..10329)
                     /locus_tag="PWY67_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2058283.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10514..11401
                     /locus_tag="PWY67_002049"
     CDS             10514..11401
                     /locus_tag="PWY67_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2058284.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(11985..12680)
                     /locus_tag="PWY67_002050"
     CDS             complement(11985..12680)
                     /locus_tag="PWY67_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDL2058285.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12664..13062)
                     /locus_tag="PWY67_002051"
     CDS             complement(12664..13062)
                     /locus_tag="PWY67_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2058286.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3917 a         2647 c         2029 g         4500 t
ORIGIN      
        1 tggcctcatt ttaggttatc atttgtaatt ttatttcaca gttgatatga ttgttatgta
       61 aaataaaaaa gtttaggata catcgaattt ttggggaaat tcttttgggg gtatcctaaa
      121 tcttttctat tatattccca tattttctat tattggtcta gacgtatgat ggtaaacttt
      181 gtacctatct gattgctgaa tcattaattg cttatcatta gccatctttg tttcaagttc
      241 gctagatgat cattttattc acatcctaac aaacttgtca catttataga ttcttaatct
      301 atttatcata caattataaa aaaggaggtg ctgcttatga aagctatcca tatttctttg
      361 ctttttttcg ctagtgcatt gattttattt gctattcatc tttgttccca gccttatatc
      421 aataacaacg gaatattagt ggaaccagca ttcttttgct taccactcgg ctttttgtcc
      481 cttcttgcct ccgcaggctt cggatttgtt gcttttttta aacgatcaaa acaacaaatc
      541 tgacttataa aatttacgta aaagagatcg tgacaataat cgtgtgatat tcccaaattt
      601 cgaataaaag gtgccccttg ccatctctaa ctcctcagtc acaagttaca atattcataa
      661 accatgaaaa gcttgtttat tgaaattgct ccttgtgact tggagctaag tgacagatta
      721 caacctcttt tgcatcataa gactttactc cttgaaaaac attactaact tttgatatgt
      781 ttcttgcgct tgtttattgc tgaaatcaaa ctgatattca tggttcaaaa cagcattttt
      841 ctccgcataa tctacttttg tgactttcac ccccgctttc tcaaatacag aagttacttc
      901 ttccatttgt ttcgtaaacg tatttgtatt tccatccgtt aagaatgtgt tttgcgtcca
      961 acgatccgca tgtctgacca aggctaaact tttgaccttc tctgattgtt caaagtcctt
     1021 attatcaaag taagcccatc cacatttttc ataaagaaag ctagagattt tatcacttac
     1081 ttgagtaagt gcagaaggat ccaacagacc agacaaagaa ataaacttcg taatctgctt
     1141 gttctccaaa gtcggatcaa gatgatcagc ttttttaatt tcttcatttt gaagtgctaa
     1201 caaatattct ccactaattt gtgcacctgc cgaatttcca ccaatcgaaa tattcgacca
     1261 atctgcttga tcttttatct cttttttagc tgcttgaatg gcttcgttca attggataac
     1321 tggtgtagga tgttttgctt ccggtgatcg ttcataatct atatttacga tgatttgctg
     1381 cgtgtctgaa catagatatt ccaaataatc acgacaatca tttttatccc ctcctacata
     1441 tgcgcctcca tgcatccaga aaagaatcgg taattttttc ttttccactt tcggtgtgta
     1501 gatatccata ttattatttt tgtactttga aggataggat atttcttttt ttatatcgat
     1561 ttgttcgatt ttttgctgga aatcatctgg tcgggcatat acttgttctt tttcacttgt
     1621 gtcaaattgt tttctaacaa gcaagctaat cggctttggt gtgatttgat taatcaaaaa
     1681 tacagtgact aaaataacta cgatgataac tggtactgca atcactattc gcttgaacca
     1741 tttcattcat ttaccctctc tttcttcgcc aaatccatgt gataatcgtg ctaacagata
     1801 aaaatccacc tgaataaatg agaagcgaat gtttcttttc tccagttttc ggcaacattc
     1861 ttttttttgc atctgtcttt ttttgtactt tcactgtcat ctgaatcggt tgcgtatcat
     1921 cacttgattg atcctctcga ggttcattct ttcccttatt gtctgcattc tctgtcggat
     1981 cttgcttttt tggtggagct actgggattt ttttgtaata gaaaataata gttggtatcg
     2041 tctcattaaa aatcccttca gtcggtcctt caacatttgc taattcgtat ccttcgaatg
     2101 tcttttgctc tgtcatataa ttttctccat aagctccgta aagctggatt tcgtcctggt
     2161 ctttgagtag acttttattg ttttcatcca catactttac gtttatctga tcatggaaca
     2221 ctactttcaa aaagtccgtt acatgtttcg tgtaggcagc atggtcaact tcctgtgcct
     2281 ttccatgccc tgctccttct actaataaaa gatctttata ccctcctgat aactcatata
     2341 atttttgcgc gttagacaca ggtactacat catctgctgt tccatggata atcaatttag
     2401 gcaatgaatc tgacattcca atcgtaggta gagcagcttt tgtatccaaa tgcatctcct
     2461 gatcaaaata gtattgtttc aaaaggccca taaataaatc ttcatgctct tgatccccaa
     2521 tgatgtctaa ctgatttttt atggcatccg gtagtgcggg ggctactgtc tggctgtacg
     2581 taaaacgtag ttgttcatca attgatgcaa atccgcaatt ttcaataatt cctttgacat
     2641 tgacaggaat atctttcgct aatacagaca tcatcgtagc tgctcccatc gaaccaccat
     2701 aaagtaaaat cttttgagat ggccgctctc gaacttcttg atttatccag taaaggacgt
     2761 catctgactc atattgtcca taggtcacat aatcgcctcc gctattacca gtcgctctgg
     2821 aatcaacaaa taataaatta tacccggcct tataaaaatc attgtattct tctgtaacga
     2881 tcccgttatt ccaattccct cgaaatccgc catggatcat gactgtttta tctgaacctt
     2941 gatcgacata atatgcatat acatccatca ttttgccatc ttgtttgttt agtacagttt
     3001 gatgaagttg ttttgtattt ggatagaagc ctgtgttttc tgtaggcgtt gtcccatcat
     3061 ctgtacctcc aagcatcgac atcaacacac tgttttttgc aaaagcgata tcgtacaata
     3121 gataagtagc aatcagatgt ttgataatct ctttatctac accataatca ttaacagcaa
     3181 gctcagtgac taataactct attaattttg acttgatgtc ttctgttggt ccattaagta
     3241 tttctgtcat ctgtttaccg aagtaattca aatattcttt accattgaaa ttttcacctt
     3301 taccgatatc atcattttct gtcccattct ctttgtcact tactttagca gaagaggatt
     3361 cgtcgtttga catttttgta gctgttgctt cttctgtttg ttggttatct gtggtagcgg
     3421 tcgtttcttg atctgtgcta gtaacagatg tctcaaccgt tgtttcagta tttgtttctg
     3481 tttgtgaagt actcgatttc gtttcatttc cagcatcagt agtttctgtg gtttctacct
     3541 cccgagattc cgattcggaa gaaatgctct ctccatatac tgtccattgc gggacgcttg
     3601 tacttacagc aaataagatg accaacgctc cctttaatac ttgttttgtt ctcaagctat
     3661 tcactccttt ttatgtttct aggagaatta tatgtaaacg caaacatttt ttatacaaaa
     3721 aataaatggt ttatcccata aaaatagaac aggattattt tctcatttta cgtaataaag
     3781 cagaatggat aaaccagtat ccattctgct ttttttaaac gaagaaacaa cttattgctc
     3841 caaaaatgtc agaaatcgtt tatcccaaat tttctcgtaa aaagcgattg tttcctttgc
     3901 tttcataaaa ggattatcat aagttgtcgt tgctcctcta cacatcataa gctggtatcc
     3961 taatccatga gcaaacttca cagtcgtatc tatacaatac tctgcttgtg ccccacataa
     4021 ttcgatcgaa tgacagtcca attcagttaa taaaacactc aaattagtct tataaaatga
     4081 attcgcatgg gtttttctca caaaataact gtgctcagga atagataatt ccgacaatag
     4141 ctcccatggt tttgtttgtg gcactaattg agcatcttcg tgttgaacga aaataactgg
     4201 tttcccaatc gtttcgtatg ttttgattct tttattcact aatgctgtca ctgtttctaa
     4261 ttcgtgtatc ggcgaagaat caaaacatac ccctttttgt aaatcgatca ctactattgc
     4321 gtcggctgtg tttgacatac ttgtgccttc tttctttttt cttgatttaa tagtatttct
     4381 tatctagcta gttccattat acgtgacaac attttcttat tttacacctc taaatggcac
     4441 ttttcgacta tttcttcatg ttattatttc ataaatactt agctattgga ggaatgtatg
     4501 atgattaaat tgtatacagg ttctggctgc tcgtcttcaa gacgcgcaaa acgttggctt
     4561 atcgtccatg atataccgtt tatcgaacag aacttaattc gtcaaccact aaccaaaaat
     4621 gaatttatcc acttactaaa attgacaaac aacggcgtga ccgaaatgct ctctactcat
     4681 tctattagtt ataaaaatct tgttcacaga atagatgaac tcagtttgag tgaactatat
     4741 attgagattc gtacatcccc ttctttattg aaaaaaccga ttatctacga ccaatctgca
     4801 aaacttttga ctggatttag cgaagataaa atcaggatgt tcctttctaa aactaaaaga
     4861 aagcaagagc tcaacgaact cttgctttct ggtcatcttg tcgattttaa agaaaataat
     4921 ctttttgcaa tgtcttaatt ttggcataaa caatctgctt ttctggatct ataatatcta
     4981 gtgttggtga cgccttctgt tgccaatact ttttcttttt tctaataaac gcaaacagac
     5041 accttgaaat cctttagaat aaagtcgacg aaaaccattc aaaggagtgt ttcaagatgt
     5101 cctattcaga gtttaccaaa gagttactag atattctaga cctaaatctt acctttcatg
     5161 aagatgcctt tcgcaaagag cgcattaatg acgaaacatg ttttgttttt gatggcacat
     5221 tgacctatca accagaggaa tgcttccatt gccactatca aaataagcaa acgatcatta
     5281 aatggggctg gaaaaaagta tccatcttat tgaatgatgt cagtaactac aagaccattg
     5341 tgcggattaa caaacagcgc ttcaaatgta aacactgtgg aaagactttt ttagcagaag
     5401 attcagttag cgatcgtcgc tgctccattg cccgcagagt caaacaagcc attcttgagc
     5461 tattaagtga gcctatttct atgtctttga ttgcacgtat gaagcacatt tctccgacca
     5521 ctgtcattcg aatacttcgc agccttcggc ctaaaaccgt ttctttgaac cagccgttac
     5581 cagaagtggt gtgtttcgat gaattcaaat cggtgaaaaa cgtctcggga gccatgagct
     5641 ttgtcatgat ggacggcaag acccatcaac tgattgatat tgtggaaaat cggcaactca
     5701 atcctttacg cgactatttc ttgcgctacc ctagaaaagt ccgtgaacaa gtacgtctag
     5761 tggtttcaga tttctataca ccctatcgca cattggtgaa agagtgtttt ccgaatgccc
     5821 gaatcgtcgc cgatcgcttt catattagcc aacacatcgg tcgggccttt accaaccacc
     5881 gaattcaagt aatgaagacc tttaaaaaag gggatcgtcg gtcgaagcac ttaaagaaat
     5941 actggagact acttcaaaag aatgcgtggg aattgaatgg tcagcatcgc tactggcgtc
     6001 cgtcatttcg agatcatctg acagaagcgg agatagtcga tcggctatta tattatgatg
     6061 actccttaaa gcgaggctac gaagtctacc aggtttttct ttccgctatc agaagacaag
     6121 atgttcctga attcgtcgcg ctcctaaaag aagactacaa ggagttgcca gagcactatc
     6181 aaccagtgtt cacgaccttc aagaaatatc gaacagaaat caaacgagcg ttgcgagttc
     6241 cctattccaa cggccctata gaatgtttga acaaccatat caaagtacta aaacgaattg
     6301 cctatggctt tagaaacttt caaaactacc gagaacgaat ctttttatat cgaggaaaat
     6361 atttcaagaa aactaagaac actacccaac tgactaaagc cagaacaaca acacggctag
     6421 acaagatagc tgtctagccg tgtgagtatc tcgattattc tattgaattt cttcaccaac
     6481 actcattgac gaagagccgc ttttcttaat taacgaaatc caatttttcc aatatggatc
     6541 tatttttctc attcctgatt tttatgattt cagctttcag acgcgcccaa tcattttttg
     6601 taggttgtcc attccagtaa aatatctgtg tattcgtttg atcatatgcc tttttgtcga
     6661 taggataatc gctaatgaca atatcaggta actgatcagc ggatgcacta tattttattg
     6721 ggcgttcagt catagataat atccttttct ttgcccataa ttcctgtgtt ttaccatact
     6781 ttgattgaat atagaatatg acaggttgtt cgtgttttat aaaaatatcc ctgactaaca
     6841 tacagtattg aaaaatcaac cgtctgttct tttcataata agtattaagc tctggaattc
     6901 tgcgagctac cccactaaaa aactgttgca tgattggata catcactgga aatatatgct
     6961 gaaaatctct gtcttctgct gattttccaa aagcatccac cttctttttt gtcccaaaaa
     7021 cccttgaata caaatggatc attgccaaat tgacatatag ataatttttt tctgaagttg
     7081 taaaacaaat cttgaagttt ttttcaaaat tttctacaat agacgattca atccagcccc
     7141 aactttcttg tactttatct ggagtatcca acaaatcttc ttccaaatag gtgttcatta
     7201 caccaaacat aaaataaagg taagcttgct ctgttggagt gatgtgattc atcgaccaga
     7261 agtttgcttc aaaaaaaggc tctagtatgt aatgcgtaaa ctcttctttt gttatcaatg
     7321 gatgcacaaa tttgttcatc tcttctggga catacgtaat cgagtggcct tgtttcattc
     7381 gttgcaacga aattcttagt aggctgatcc atttctttct atctatacta cgcgtatatg
     7441 gcgcatgttt cgcaaatttt tctaatagtt gacgtttatt ttttatactt gataatatat
     7501 cagaatgatt ttcgtcgaat gactgccaat acatcaagtg aaaaaagtat cggatacggt
     7561 attcttctcc ttctatcgga ttaccttttt tcaagctgca agataaatga tagtttttca
     7621 aaatagtatt aagttctctt aattttcttt gaatcgtacg caaacttata taattttttg
     7681 actaagattt tctaaggatt gataagtacc tttgtaaagt gaatccagta aaatataacc
     7741 aatcgttccc tctaaaagta tagaataaac tttactcagt aaaaactgag aagaaaactc
     7801 gacttttatc atcttgtttt ctgaaagaaa agataattta tattctggat acctttcgca
     7861 aaacgattct gcttgttgaa tcaccgaatt catcttagcc agggtgtagt ttagatctaa
     7921 agcgatatcc ttcaccacat ataatttctc gtctctttca taacaatagt tcaaaaattg
     7981 atatacttgc ctttcattga tatcaataaa ttcttcaaac aaagtacata ctccttttta
     8041 tctattattt agacttattc cgacagctac atgaataacg tacaaataga ttcgttactg
     8101 atttttcaga gatactacca tgcgcagtgt gcattgtcaa ttattcttgc tcttattttc
     8161 catttgtgtt cctgttattt cctgtttctc aaatatgtta gtaaacaaaa atagatctgg
     8221 caaagctaca acttcgccag gcaatacagc actctcgaat gaaatattca ctcaaaaatc
     8281 tatcgtttta ttcaatttta gatctgttgc ttttattctt tatttcactc gatggaagag
     8341 agaacgaaaa tagcttgctc ctacaatcaa aatgagtaac acacccatcc atgtaattag
     8401 cttgttcaag gttctactac cagtttctgg taaatcgctt tttctattat ctgtttcttt
     8461 cgctttttct tgatgatcaa ttccacgaga aacaaaatca ggctgtttta gcacactttt
     8521 taggttgctc cctgcatttc tcttttgttc cttagttggt atgataggta ccaaatcaag
     8581 ttcattattt acttttcctg tgctgctttc gctgctcgtg cttcctgtgc tactctcact
     8641 actcatgctt cctgtgctac tttcactact cgtactctcc gtactgcttt cgctgctcgt
     8701 gcttcctgtg ctactttcac tactcgtact ctccgtactg ctttcgctgc tcgtgttttc
     8761 tgtgctactt tcactgctcg tactctctgt actgctttca ccgctcgtgt tttctgtact
     8821 gctttcacta cttgtgctat ccgtactgct tggcggtcca acaactgtcc aactgcttgg
     8881 tttcaaattc tccgtgatcg tcatctgtcc atcataaaaa acaatcaaat caactttatc
     8941 tttactcttg atctcatcaa tcgatagttc tgcagaaatc attattgtat actcaatacc
     9001 agcggtccat tgtacgtctt ttaaataaac ttccccaata actttttctg aacttccatt
     9061 accatcccaa ccatatgtta gaacagcatg tacatcacta gcattcacgc ttttgtttgg
     9121 tttgactgga atagtaatga cttgttgtgt catgtcataa acaatccgat cagttttcac
     9181 agtaaatgaa agattctcat ttgcccgttc atttgcagaa gtactgattc caatcattgc
     9241 tgcaattata ctcagcccaa gaataacaaa taatacggtc gctgttttct tcatttctat
     9301 cactctccat ataattacct tctatttgat aaaggaatta tataaaatga aaatgatgag
     9361 aacacgacag tcagttgtga aaatctaaca aaagatgtcg tgtattcgct tgaatttgtt
     9421 tactttttcg tttaatcaat gacagactta aaaaagcaag accgatatca gccttgcttt
     9481 cttcatcttt tattgataaa cagcagtgta tttcttcatc cgctttgttt tatatgcata
     9541 gagtaacaaa ccaacaatca agactaacaa gaataatgta gttgatatag atgcttcagc
     9601 cccaaagttt cccccagtaa ttattgtatt ttgcgattgg atcagagtgg agaaaaaacc
     9661 ttgttcttgc gttccgctga ctggtatacc caaaatagct ccttgtgtcc aattccaagt
     9721 aatatgagca ccaatagccg taaataaact tcctgtactg tagataagtg cgctgaaaaa
     9781 gcaacctgct aaaaagatat tgaaaactgc cagaaatgtc atgttaggat tcaatccgtg
     9841 caaaagagca aataatgcag cagatccaat cattgctatt aacggaccaa atctaagcat
     9901 ctgatgctga aaaaagccac ggaataaaag ctcttctgat gcccccactg caataaataa
     9961 aataaaactc gttatcagcg gcacaatgtt tgcgtttcct gaatctaacc gatacgcccc
    10021 cgtcaaatat aatggactag taataagcag aaacactcca attccaacaa cgagacctcc
    10081 cgctaaatac ccccagtctt ttcctcgaac agaaacacca taatcttcta ttctttttcc
    10141 ttcgacctta ctagcaagaa ataaagctag taaaaaaaca gctaaatgtc cgaccgtacc
    10201 ggcaattgct ccaatccatt cactatttcc aataaacata tccgcaagtg cattattgac
    10261 aagaattcct aaaacaaaac caactaagac tattaaaaca gatccaatca accgaataat
    10321 cctgatcatc tttcatccct cctttatatt aagtttaaca acccttttct ttcttgtctc
    10381 gtgaaaacta tgtaaatgtt ctccatcata gagagaatag atttttacga actaagaata
    10441 tctacagcaa ccttacgaaa atgaaagact gtctccctaa ttcttctgtt agactgtttt
    10501 taataaggaa aggatgggaa attttttgaa aaaacaacta ctaattatgt gcctttcagc
    10561 atgtgcaatt ttactattct caggttgtag caagtccaat gaaacgattg caacgtttaa
    10621 aggtggaaca cttacagttc aggattttta tgataaccca aaagttaaat caaataatca
    10681 aacactatta aaaaaaatga ttgtcttcaa agcatttgat gatgtatacg gaaaagagat
    10741 ttcttcaaag gaagtaacaa aagaatataa tgaacaaatc aaaaaactcg gtccaaacta
    10801 caaggaacaa ttaaaagcag ttggccaaac agaagagaca tataagaaac tctttaaaca
    10861 gatgctggct ttccaatacg gtttgaaagc caatgtaaaa ttaacagata aagatctaga
    10921 cactgcttgg aaagaattct atccagaagt tagtacacag atcattctat tttcgactaa
    10981 agaagaagca gataaagcaa aaaaagaagc aaatgaaggg gaaaatttct ccaagcttgt
    11041 tcaagcttat ggaaaaaata aaacattaaa ggaaactgat ggaaaaatga attttgattc
    11101 aaccaatcca gagattccta ctgaagtaaa aaaagcagca tttaaactaa aaaacgggga
    11161 agtttctgat atcattcctg taacagatcc aacgacttat cagcaaagct attatctagt
    11221 aaaaatggta aaaaaacagg ataaaggttc taataaagac aaatacaaat ctgagctaga
    11281 aaaaattgct accgaagcaa aaactcttga tacggaattc atggataaaa caatccgtaa
    11341 agtgatgaaa aaagacaatg taactattaa agatccttat gtaaaaaata tattcaaata
    11401 aacaaatttg acccaattgg ggatgtggca aaatttgaga agtgattttt ggaaattcct
    11461 tcttattttt gaggcaaacc acttttgcca caacttcatc ggggcctttt tttatttgta
    11521 ccaaaacaaa tcactggatt tgttacaatc atgaggatat aatttaaaaa agttttttat
    11581 ttctatcaca tgaatcaatg cgccattaag tagattcttt catgcttccc tattttttca
    11641 cttgccgtat cgcttcaaaa acaactatca taaactcaaa ctcgactgaa aagttatttt
    11701 aaaaaagact aaaaatatcc cttctggact tgttcctctt attctgttat actagagaaa
    11761 cacgaaagat atttttatgc gacacttttc atttgttttt atgaaattta ttcaaaactg
    11821 gcgtaacttc agagttacaa ggatgtatta gaggttaggg aatacatcgt ttaaatgaga
    11881 aataagtaat agtcacccaa actttaaatt aatgaatatt gaatcgtcag atagcctcct
    11941 cgagttttcg agaaggctat cgtttatttt atggagaaat tgattcagaa gtaatcagcg
    12001 acctttttta gttcaccaat cgtttctgct tggaaagttt gcacccaatc ttcttggaag
    12061 cccctcactg ctttatcaat cgagggcatc tctaatactt gatctagtag ctctttacct
    12121 aacgttatcg catgagctcc agatcgaatg gctgtagtga cttgttcaca atttttgaaa
    12181 ctagctgcta atatttttgt tggtctttgc gtacgttcga tctcagtccg caacgaatca
    12241 atcactttca tagcatcaat gccttgattt tgcatcctgt tataataagg tgcaatataa
    12301 tctgctccat tattaatagc taaataccct tgtaattctg tgtaaatagc tgtaccggta
    12361 atcaaataac cttctttttt caactttttg atcgcttgaa ggcctgcttg attcaccggc
    12421 accttgataa acgtatcttt tcccagttct tctatgataa ctcgagcatc ctctatcatt
    12481 tcttcttccg ttcttcctat tgtctgaata tggataggaa tctgtccgat tatttttttg
    12541 atttctttca tatgcgagaa gaagtttgac gctccttctt ttttcagaat tgtaggattt
    12601 gttgttacgc ctgttaaatt caaaaactgt tgacaagaat aaatactttt tgtatttgct
    12661 gtatcaagca tgatttccat atagctttcc cccattctga tcctcttgat cgattttaat
    12721 gattgtcccc tttcttaaat gcggcatttc cttatcttct acatacattg ttcctggaag
    12781 ttccggctca tgagatccat caaattttat cgtgatatgt cctaattcat tcaaattagt
    12841 ttggacaaca tctcctactg ctgtgattac atacatttgt ttatcaaaac agagcttcat
    12901 atcacaagtt atttcttttc ttacctgatt caattcaatc ttataacaat aatctgttaa
    12961 tgtttccggt gcttcatctc caaataaaat cattattttt tctttcctga atacttctgc
    13021 ttctatacca acatctgtta caaccgtttc aaagatcgtc atctctgttc ctcctcgtaa
    13081 aatatcattt gtc
//
LOCUS       DANKAZ010000074        12858 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000074 DANKAZ010000000
VERSION     DANKAZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            133..3660
                     /locus_tag="PWY67_002052"
     CDS             133..3660
                     /locus_tag="PWY67_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL2058287.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="PWY67_002053"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="PWY67_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL2058288.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="PWY67_002054"
     CDS             7448..7789
                     /locus_tag="PWY67_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2058289.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="PWY67_002055"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="PWY67_002055"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL2058290.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="PWY67_002056"
     CDS             9146..11566
                     /locus_tag="PWY67_002056"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL2058291.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="PWY67_002057"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="PWY67_002057"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL2058292.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4360 a         2240 c         2728 g         3530 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataacta
//
LOCUS       DANKAZ010000075        12719 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000075 DANKAZ010000000
VERSION     DANKAZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..577)
                     /locus_tag="PWY67_002058"
     CDS             complement(<1..577)
                     /locus_tag="PWY67_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2058293.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
     gene            719..1651
                     /gene="pfkB"
                     /locus_tag="PWY67_002059"
     CDS             719..1651
                     /gene="pfkB"
                     /locus_tag="PWY67_002059"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2058294.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            1633..3096
                     /locus_tag="PWY67_002060"
     CDS             1633..3096
                     /locus_tag="PWY67_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2058295.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            3093..3551
                     /locus_tag="PWY67_002061"
     CDS             3093..3551
                     /locus_tag="PWY67_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2058296.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            3548..4522
                     /gene="lacD"
                     /locus_tag="PWY67_002062"
     CDS             3548..4522
                     /gene="lacD"
                     /locus_tag="PWY67_002062"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2058297.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            4963..5691
                     /locus_tag="PWY67_002063"
     CDS             4963..5691
                     /locus_tag="PWY67_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2058298.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            5709..6905
                     /locus_tag="PWY67_002064"
     CDS             5709..6905
                     /locus_tag="PWY67_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2058299.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            6898..7893
                     /gene="lacD"
                     /locus_tag="PWY67_002065"
     CDS             6898..7893
                     /gene="lacD"
                     /locus_tag="PWY67_002065"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2058300.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            7890..8831
                     /locus_tag="PWY67_002066"
     CDS             7890..8831
                     /locus_tag="PWY67_002066"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HDL2058301.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            9007..9486
                     /locus_tag="PWY67_002067"
                     /pseudo
     CDS             9007..9486
                     /locus_tag="PWY67_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            9499..10389
                     /locus_tag="PWY67_002068"
     CDS             9499..10389
                     /locus_tag="PWY67_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2058302.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            10376..11185
                     /locus_tag="PWY67_002069"
     CDS             10376..11185
                     /locus_tag="PWY67_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2058303.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            11199..11600
                     /locus_tag="PWY67_002070"
     CDS             11199..11600
                     /locus_tag="PWY67_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2058304.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="PWY67_002071"
     CDS             complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="PWY67_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2058305.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         4072 a         2056 c         2780 g         3811 t
ORIGIN      
        1 atttaaaaaa ttgaaaaata aagacataaa tcttattttt gatgttttta tttgttttct
       61 caccgttcaa agaaacaaaa ataacgctgt ctttcttagt tagtttccga ctgatgcttt
      121 tgataatttc tggatcagta tgcatctgac aattttttcc cgttaattga agttttacca
      181 gcatttcttg gccaatcatt tctgaaaatc ctcgggcaaa gacaaaaacc cggtcagcaa
      241 atttgattct ttgtattgca tcttcaatgt tcccgatttc caacatattg attgtacgta
      301 ccacttcttg ttcatttttt agaatggctc ggcggattcc tttgtctact ttatctaaaa
      361 atgaaaaatt gatgttcttg ttttctcctt cttttagatg atgcttaaac gaagtaaacc
      421 cttcatagcc tttctttttc attgtccgca caatggtggc tgttgagaca tttgcttgtt
      481 cacttagttt cacgatggaa tagttaggga tctctaggat gaggtgttcg ataaagttcc
      541 agaggtactg ctctgtttca cttaatttgt gattcatatt ctttccttct ttcaccaaaa
      601 gttagttaaa aaaagcacga cgagtttata aatgtaaagg ttttctttgt gttgggacga
      661 ttaagaaaac attttctatt tcgcgtattt cataatagaa tcaaggaggt cgttggttat
      721 gtctatctac acatgcacga tgaatttggc aattgatcta tttattgaaa cagatgagat
      781 gcatcctttt atggtaaacc gcacaaaaga ggatgacatt caggcaaacg gaaaaggggt
      841 gaatgtgtca ctagtattaa agatgctaga tttcccaagt acggcattag gtttttctgc
      901 tgggttcaca ggaaagtata tagaagacta tttacagcag aaacaaatcc aaacagagtt
      961 tattgaagtg cctggtatga cgcgtatcaa tgtatttaca caagtcaatc aaacgggaga
     1021 agaatacaaa ttagtgaatc aaggacctga aataccgcag acagctgttc ataatttttt
     1081 aaatcaaata agagcattac aagcagggga ttatctttgt gtttctggga gtttaccaag
     1141 agggctgtct ccaaaggttt taatagaaat cagtcggatt tgtttgatta ttgatagtag
     1201 tgatcaagaa gtcatggatt gtttacccta tcgaccgttt ttgctgaaac caaatgagga
     1261 agaactttct tcttggttcg gacgaaaaat ggttacagat gaagattact ttgtttatgg
     1321 acaacgacta gtagagtcag gcgcagaaaa tgttttgtta tccctcggtg agaaaggagc
     1381 gattcttttt acgaaagaca gggtgattcg aggaaactcg ccaaaaggaa aagttgtcaa
     1441 tacagcatgt gcaggtgatg ccatgttagg cactttttta gcaggataca tgaatcgacg
     1501 atcgcttgat gaaacgttac gtaaaagcat agcagcaggc agctctactg cttttcgtaa
     1561 aggactgact gattttttgg atgtggagga attaagtaaa caaatcaaaa tcaaggaaga
     1621 gaggttcgaa agatgggaaa atatcaacta attgcagcaa ccggttgtcc gacaggaatt
     1681 gcgcacacct acatggcgca agaggcatta gagcaagcag ctaggaaaaa aggcatcacg
     1741 atcaaagtgg agacccatgg acaaattggg atcgagaatg aattaacggc tgaagaaatc
     1801 aaacaggcag atgctgtcat tattgccgca gacaaagatg tccattcaga aagatttgtg
     1861 ggaaaacgag tcattgatgt ttctgtcagt acaggaatca aggatgcaca acggctgatt
     1921 gacgaagcat tggcaggaaa aggtactgtc ttagcagata atgaagctgc tgatacttta
     1981 gaaccagaag cgataagtgg tgctaatttt ggtcgtagca tctataaaaa tttgatgaac
     2041 ggtgtctctc atatgttgcc tttcgtggta gctggcggag tattgattgc tatttcattt
     2101 gccgtttggg gaatctattc ttctgatcca gatcatgcac aatacaatgc tacagcggca
     2161 atggtaaaag cagttgacga tgcagcaatg ggaatgatgg ttccagtttt agcagcttat
     2221 attgcagaag ggatcgcgaa acgtccaggg ttagtcgttg gatttgttgg tggaatcgta
     2281 gccatgaatg gcggtactgg atttttagga ggtattttag ctgggttctt agcaggttat
     2341 tttatcttat ttttgcaaaa gctactgata attttaccta aacaactaga tggcttgaag
     2401 gcaatcgtct tatatccagt tattggtgta gcatttattg gcatcgtgat gtctttgctg
     2461 gcaggtccaa tgaaggctat aaatgaagga atgatgagct ttttagctgg atttgaatat
     2521 tcgaatccac ttgttcttgg cgtgattgtt ggctgtatgt gtgcgtttga tatgggagga
     2581 ccggttaata aggcggcata tgtaacagga acagctcttt tgacacaagg aaacacgact
     2641 tttatggctg gtgcgtcagc agcttgcatt gcaccaccgt tgattactgg ttttgctact
     2701 ttattgtttg gtaaatattt tgatactaat gatcgtaatg caggtttggt taattttatt
     2761 ttaggatcaa ctcatattac ggaaggagct attccgtttg ctgcgaaaga cccgatccga
     2821 gtattaccaa cgatgatgtt aggctcatca attgctgctg tcttgaccta tctgtttggt
     2881 gtccaagtac cagccccaca tggcggtttt ttagtgttac ctgtagtgac gggaaaaatc
     2941 caatgggtta cagcgattct tattggttcg atcgttggtg gagtgatact aggtttgatc
     3001 caaaagaaaa gagtaactga taaagacaag aagagggata ataaagcaac aaatcgtaca
     3061 gttatcgaag aaggaagagc aggaggttta tcatgacaat cattcatgaa gagaacattt
     3121 ttttacaaca gccattagat aatcaagaag aagcatttaa gtttttagca aagcaaacat
     3181 tctgtttagg aatcgctcat gacgaaaaag aagtatatca aaaacttctg gaaagagaaa
     3241 aagaaggtac tacaggaata atgtcaggtt ttgctatccc tcatgcgaag tctcaaacaa
     3301 tcaatgaacc tagtttgatt atcgtcacac tgaaaaaagg aattgaatgg aacagtttag
     3361 atagacagtc agttgatttt atttttgcgt tatttatccc agattcagaa gcaggaacga
     3421 ctcatttgaa attattgtct ctcgttgcac gatcattgat gcgaaaagag gttacagaac
     3481 agttgagagc tgcaaaatca aaaatagaaa tagctacaat attaactaaa gaaataggtg
     3541 aagatatatg aagaaaatga gtatagaaaa acaggaacgt ttgaagcgat tgacggatga
     3601 agctggaatt attagcgcgc tagctatcga ccaacgtggt gctttgaaga aaatgatcaa
     3661 ccaatataaa gaagctacat cagaagatat catccaattt aaacagatgg tttctcagta
     3721 cctgactccg tatgcttccg ctattttact cgatccagaa tatggtctgc ctgcagcagc
     3781 gcttcgagag aaagaatgtg gtttgttgct agcttatgaa aaaaccggct atgatgcgaa
     3841 aatgccaggc cgactaccag atttactgaa tagttggtca gtgaaaaggt taaaagaagt
     3901 gggtgcggat gcttgtaaat ttttattgta ctatgacgtt gatgaaccag tagagattaa
     3961 tgtgcaaaaa caaatagtta tagaacggat tggttcagaa tgcgaagcag aaggcatacc
     4021 tttcttttta gagctccttt cttacgatgc aactattggc gatccgtctt ctcttgacta
     4081 tgcaaaagta aaaccgcata aagtgattga agccatgcga gaattttcaa aagaacgtta
     4141 tcaagtggat gtattgaaag tagaagtacc agtgaatatg agatttgtgg aaggatatgg
     4201 gcaagatact tgttatagtc aagcagaggc atccgcgtat ttctatgaac aatcgaaagt
     4261 gactgaacaa ccgtttattt ttttaagtgc gggagtcagc gctgcactat ttcaagatac
     4321 attgcgtttt gcaaaacaat caggttcgac attcaatggt gtcttatgtg ggcgagcaac
     4381 atgggcgaag ggagtagcac cttttgttac cgaaggcgaa gaaaaagcga gtagttggat
     4441 gttagaagaa ggaaaacaaa atattaaaga attaagtaca ctattagaag aaacagcaac
     4501 ttctatttat gttaacaagt aagagaaagg attgtctgag tgacagattc aaaaaataat
     4561 tagttattta aaaaaatgtt tgtcaaattt tatagaaaaa aagtataaaa ctgcaaaagt
     4621 tatctaaaac taataataac atttgtcatt aataagctat ttttagttgt tgtaaatgat
     4681 attggaagta ataaaacgat agttgtagga atatcatagg gattaatgtg gatcacgctg
     4741 ctcgataagt tagaccattt tgacggatcg cttatgtggt tcgtcttttt ttctgttctt
     4801 ttattggtaa ctggtatttt tgaggtatct ttgtctattc aagatataaa gctttaaaga
     4861 gctgttataa tagctttttt ggtctaaaaa acctttgaca aatgattaat aattcactat
     4921 tcttgtgttg tggttataac cagtaaagaa gaggtgattt ttatggagag ctcaaataaa
     4981 ggaaaatcat tgtattatca actagttgat ttattgaagg atcgtatcga aacacagatg
     5041 actccgcatg ataagctgtt atctgaaaga gaactttgtc aacactatgg ggtgagtcgt
     5101 accaccgtac gtctggcttt acaggaattg gaaaatcaag gttatattta ccgaagacat
     5161 ggaaaaggta cctatgtctc tgatttgtct gataagtcat cgaacttagc tggagcatat
     5221 agctttacag aagaaatgcg cgaaataggg cgcgtcccta agacggtcat tttagaattt
     5281 agacaaatgg aagctaataa gagaattgct aagaatttga acatccattt aggtgaaact
     5341 gtatttaaac ttaaaagatt gcgtttggca gatgaagagc cattgatggt tgagcgttct
     5401 tatctaccgg cgaaactatt tatgggactt gatttgccgt tacttgaaag taaaccgttg
     5461 tatgatgtgt tttcacagga ttatgaacaa caagttcaat tagctgatga agagttttat
     5521 gcaagtatcg tgcaaaaaaa agatgctgtt gtgctacgaa tcgctgatgg cgctcccgtc
     5581 ttacatctga cgaggataac ctataacgtg aaaaatgaga ttatcgaata tacagatagt
     5641 gtagcaagag cggaccagtt tcattataaa attcggcata tccgaaaatg attgagaggg
     5701 agaacaacat gtttgagcta tcgaagaaca aattaaaaca aataggtgca gcaatcacga
     5761 cacaagaaat aaaacaacaa ccagatctct ggaaagaaac aatcgataga ttgatgcaaa
     5821 gccaagaaaa tttagctcta ttttttgatc aaataaaaaa agaagcaaac ggaagaagaa
     5881 tacgtgttat ttttacgggt gcaggaacgt ctcaatatat tggggatacg ctcttaccct
     5941 atctaaatga acatggagac aatcagtttt ttatcttcca cagttttggc acgacagata
     6001 ttgtagcttc accaaaagaa tttttgtttc caaatgaacc gactttatta gtttcatttg
     6061 ctcgcagtgg caacagtcca gaaagtgtag cggctgtgca aattgcaaat gaacaagttg
     6121 aaacattgtt tcatttgacg attacttgtg ctaaagaggg gatgttggca aaaaacgcac
     6181 aggatgataa acagaattat ctgttattga tgccagagag agcaaacgat gcaggttttg
     6241 ctatgacagg aagttttacg tgtatgttat tgagtgcttt atggatcttt gatcaaacag
     6301 caatggagaa aaaagaaaga tatcttgaac aattagttaa aatcggtcaa gatcttcttt
     6361 caagagatca agaaattcaa aagttattac ctgatttttt tgaacggatc gcttatctgg
     6421 gttcaggaag tttggcaggt gcgacaagag aagcacaatt aaaaatttta gaattaacag
     6481 caggtaaagt tgctacaatt tttgattctt cgatggggtt tcgtcatgga ccaaagtctt
     6541 ttttaaatag cgagacactc gttgtaggtt ttgtgagcaa tcaaccatat acgcgaaatt
     6601 atgacttaga tattttagag gaaataaaag aagatcgggt cactgccaat gtcattgcga
     6661 ttgcgcaacc gggtactatt gatttctcag gtatgactgt gctattggaa gactcggtga
     6721 gtaaattgcc cgatgcctat cttgcattag cgatgattat gactgcacaa atcattgcac
     6781 tctatacttc aatcaaagta gctaataaac cagatacacc atctccaaca ggcacggtca
     6841 atcgagtagt caagggcgtc attattcata agtatgaaac cgaaggagaa atgacagatg
     6901 aatgaagaaa aaaatagggc aatggagaga ctatcaacaa aagatgggat tattagtgca
     6961 ttagcgatcg atcagcgtgg tgctctaaaa aaaatgatca aatcgttggg aaaagagcca
     7021 agtgaagacc aaatcagtga attcaaagaa ctagtatcag ctgagctgac accctatgct
     7081 tctgctattt tattggatcc ggaatatgga ttgccagcag caaaagtcag agcaaaagag
     7141 acggggttat tgttagctta tgagaaaacc ggctatgacg caactacgcc tggtcgtcta
     7201 cctgatttat tagcggattg gtcttctctc agattgaaag aacagcaagc ggatgcagta
     7261 aaattcttac tttactacga tgttgatgaa gcacccgaaa tcaatcattt gaagcatgtg
     7321 ttcatcgaac gtttaggtag tgagtgcgaa ggagaaaatc gtcccttcta tttggagtta
     7381 gtttcttatg atgcacaaat aagcgatagt tcttctccag agtatgcgaa agtcaagccc
     7441 cacaaagtta ttgagatgat gaaagaattt tctgatccaa aatataaagt tgatgtgttg
     7501 aaagtagaag taccagtcaa tatgaattat gtgaatggct attccacagg tgaatttatt
     7561 cacagcaaag aagaggctgc tgccttcttt aaggagcaaa gtgaagcgac tccattacca
     7621 ttcatttttt tgagtggggg tgtttcaaca gaattatttc aacaaacact gatttttgct
     7681 catgaatcgg gttccacctt caatggtgta ttgtgtggac gtgcgacatg gaaagatggg
     7741 gtcaaagcat acgtcgaaaa tggaaaagac ggagctattc attggttaag aacaaatggg
     7801 agagaaaaca tcgagacttt aaataaagta ataaatgaaa ccgctacccc ttggttcaat
     7861 aaaacaaaaa gcaaaaaggt gaagatgaaa tgattacgac agttaccttg aatccttcca
     7921 tcgatatcgc gtatacaatt gaagaattaa acctcgacaa agtcaatcga gttgaaacag
     7981 tcaataaaac agcgggagga aaaggagtga atgtggcgag ggttctaaat ttattaggag
     8041 aagaggtgat agctactggt ttaataggtg gccagcacgg tgaatatcta aagaaccagc
     8101 tagatcagtt agggatcatg catcagtttg ttccaattaa aggagagaca agggattcta
     8161 ttgctatcct gcatagttcg agacaaacag agcttttgga acgaggcccc ttcataacaa
     8221 agaaagaaca acagacattt accgatcatt tcgagcagct cctcgaaata tcagatatta
     8281 tgacgatctc aggtagttta ccccaaggat gccctcttga ttactataag caattggttg
     8341 ctttagctga taaaaaaggg aaagacatat tactagatac ttctggtaaa tttttgcttg
     8401 aagtattgga aggtaaggag aagccactac ttatcaagcc caatctggaa gaattaggag
     8461 aactgattgg ccaacgtatc tgttcttcga ttcaattaga aaaaattcag tccttattag
     8521 ctcatccaat atttgaagga gtgccatggg ttgttgtttc tttaggagag agaggtgctg
     8581 tggcaaaagt tgctcagtgt ttttacgaag tcattattcc taaaatatca gttgttaatc
     8641 ctgttggttc aggtgacgcg acccttgcag gatcggccca tgcgttatca aaaaggcaat
     8701 caatcgaaga gattctcgca ttttctatgg cttgtggtat ggcaaacgcg caacaagaga
     8761 aaactggctg gatcgaccct atgaaagtta cgatgttgat gaaacacatt caagttaaga
     8821 aaatcaaatg aacttaaata taaaagaaag cgttttcttt agttgggaag gaatttgttc
     8881 ttagcttaaa aatgaaatat aagaaagaaa aaatgaaaac gttatctttg aaacagaagg
     8941 gatatttgat gatgtcacag ttttaattga acaataaata gaaaaaatag ataaggagaa
     9001 tgcatgatgg caattattgg tgtaagaatc gatggacgtt tgatacatgg ataagtagca
     9061 aatctttgga caacgaaact gaatattagt cgaatcatgg tcgtggatga tgaggtagcc
     9121 acaaacacaa ttgaaaaaag tggattgaag ttagcgactc cagcaggggt aaaattaagc
     9181 attttgccaa tcgaaaaagc agcaacaaat attttagctg gcaaatatga ctctcaacga
     9241 gtattgatcg tagcaaaaaa gccagatcga atcttatctt tagtagaaaa aggagttcct
     9301 atcaaagaag taaatgtggg gaatatgtca caaacgaatg aaacacggtc cattacaaaa
     9361 tcaatcaatg tggtagatgc agatatcgaa gtatttaaaa aattagatca gcttggtata
     9421 catttaattg ctcaaatggt accaaacgat aaagcagaag actttatgaa tcttttaatc
     9481 aaataggagg aatgatccat ggatattcaa tggtggcaga tcttattatt aacattatat
     9541 gcaggttatc aaattttaga tgaattacag atctattctt caatgagtgc gccagtgttt
     9601 gcagggttat atgcaggttt agtaatgggt gatttaaacg caggtttgat tattggtggc
     9661 agtatgcagt tgacagtttt aggagtgggg acgtttggag gcgcatcaaa aatagatgcc
     9721 aactctggga cgattttagc aactgccttc tcgatttctc ttggaatgaa tccagaacaa
     9781 gcgattgctg ctattgcagt gcctgtagca agcttaatga tccaattgga tattttagct
     9841 agattcgcta acacatactt tgcccatcgt attgaccgtc acgtagaaga catgaattat
     9901 caagcaatcg aacgcaattt cttaatggga gctttacctt ggtcgctctc tcgtatgatt
     9961 cctgtatttt tagctttagc atttggtggt ggattagttg aaaatgttgt ttctgtttta
    10021 aacgggaatc taaaatggct aggcgatgga ctttcagtag caggagctgt tttaccagct
    10081 gttgggtttg ccattcttct gcgctattta ccagtaaaaa aacatctatc ttatcttatt
    10141 ttaggattta cgatcacggc attattagca acgttattct ctaatgttca attattagga
    10201 acaagtgtgg caagcgttgt taaagatctt ccggcaactt tcaattcgct gccgatgctt
    10261 gctgtagcat tgattggtct agcactagca atgatcagtt ataagaatga tcaaaacaaa
    10321 gaccatcaac cattagctag tgagccatca cattctgttg agggggaaat cgaagatgac
    10381 gaaatctaat tacaaattaa caaaagaaga ttttaagcaa atcaatcgaa gaagtttgtt
    10441 cacttttcaa ttaggttgga actatgagag aatgcagggt tctggctatt tatatactat
    10501 cttgccacaa ctaagaaaaa tatatggaga caatacacca gaattaaaag agatgatgaa
    10561 aacacatacg caatttttta atacttctaa tttttttaat acaatcatta ctgggattga
    10621 tcttgcaatg gaagaaaaag agggaattgc ttcaaaggaa accgtatcgg ggctgaaagc
    10681 tggattgatg ggttcctttg cagcgattgg agattccgta tttgctgctt tgattccaac
    10741 gatttttggg gctttggcgg cgaatatggc aactgatgga aatccgatag gtgtgtttat
    10801 atggattttt gctcagatcg ttgtcatggt ttttcgttgg aagcaacttg aatttgcata
    10861 caaagaagga atttccttag ttactactat gcaatctcgc ttaactgcat taacggacgc
    10921 agccacttta ttaggggtat tcatggtagg ggcattagtt gcgacaatga tcaatgttaa
    10981 attttcatgg gcaccaacga ttggcgatgt gacattgaat atgcaaaact atatcgatat
    11041 gatcttacct aaaattttac ctgctgggat cgtcggtggt gtttattggt tattaggaaa
    11101 gaaaaacatg actcctactc gagcgatttt tatcgtatta attgtgtgtg ttgctttatc
    11161 tgcattgggt gtgatttcga aataagggag agcgaaaaat gagtagacaa ttggtattga
    11221 ttagtcacgg aaaattttgt gaggaattga aaaaaagtac agaaatgatc atggggcctc
    11281 aagaggatat ctacacgtta cctttattac cagaagatgg cactgaaaca tacaaacaaa
    11341 agtttctcac tgtcacagat tcgttaaatg attttgtggt actatgcgat cttttaggtg
    11401 gtacacctgc aaatatcatt tctaaactaa taatggaagg acaacagatt actctttatg
    11461 ctgggatgaa tcttccgatg gtcattgaat ttatcaactc acaaatgatt ggttcagagc
    11521 ccgagtttgt tcgtgcagga tgtgaaagca ttgtctctgt caacgaatta attaatggaa
    11581 tagcagaaga ggaagagtaa caaaaaagat gagggcttag ctctcatctt ttttgtccta
    11641 tgtttttatt tccgcttctg ctgctttcct gcgttgcggc ctttgttgtt tgtactgttt
    11701 tttggttgtt tgatttgttt tgttgcttgt ttgttgttag ctggctttgc tgattctgtc
    11761 acgtcttttc ttggagtgac gacttgtttt ggcgggtttt gtttcaactc ttctgccact
    11821 tgtgctttca aacgtggttt catcataacg tttgtgatga atgtttgcag acagctgaac
    11881 acaccaccga ctacccagta aagtgtaaca ccggctggcg cactgatcga catgaagacg
    11941 atcatagcag gagagacgat tagcatcgta cgcatcgtct ttttctgttc ttcaggaata
    12001 ccgatcgttg agatgtatcc ttgaagcaga taggcgaccc ctgcaatgac aacaagtacc
    12061 atacttggtg agcctaaatc gatgccgtag aaagatgatt cccgaatacc ttctgtataa
    12121 cgtgccgtaa agtacaaggc agagaagatc ggcatttgga tcaatagagg cagacagccg
    12181 attccgccgg tcatactcaa tccgttttcg cggtatactt gctgcatttc tgcttgtgct
    12241 gccatttgtt cttcacgtgt tgttgcagct ttcaattttt gttgggctgc ttcaacttgt
    12301 ggttttaacg cttgcatttt ttcagattgg atcattgttt ttttcgattg actaatacct
    12361 agtggcaaga tgatgatacg gacgatcaca gtcattgcaa taactgccca gccataagac
    12421 caattgaaat tctcaactag atatgtaatc gcttggccca taggatggac gagtactcga
    12481 taaacgattc ccgaagtgtc agggtttccg tttgaatcca tccgcacaca gccagataaa
    12541 aagagcagga tactgacaag tccggatccc aataaccagt ttttccattt attcatagaa
    12601 tacttcctct tctataattt ttacacaaac ttaactatac taaaaaagcg catggaattc
    12661 aatcaacttc atagaaattt catataaata ttcgttcttt tacgtagcga aaaattttg
//
LOCUS       DANKAZ010000076        12308 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000076 DANKAZ010000000
VERSION     DANKAZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            288..695
                     /locus_tag="PWY67_002072"
     CDS             288..695
                     /locus_tag="PWY67_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058306.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="PWY67_002073"
     CDS             709..1110
                     /locus_tag="PWY67_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058307.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="PWY67_002074"
     CDS             1112..1483
                     /locus_tag="PWY67_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058308.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1818)
                     /locus_tag="PWY67_002075"
     CDS             complement(1519..1818)
                     /locus_tag="PWY67_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058309.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1824..2054)
                     /locus_tag="PWY67_002076"
     CDS             complement(1824..2054)
                     /locus_tag="PWY67_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058310.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            2671..3333
                     /locus_tag="PWY67_002077"
     CDS             2671..3333
                     /locus_tag="PWY67_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058311.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            3412..6637
                     /gene="fss3"
                     /locus_tag="PWY67_002078"
                     /pseudo
     CDS             3412..6637
                     /gene="fss3"
                     /locus_tag="PWY67_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            6751..7065
                     /locus_tag="PWY67_002079"
     CDS             6751..7065
                     /locus_tag="PWY67_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2058312.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            7078..7452
                     /locus_tag="PWY67_002080"
     CDS             7078..7452
                     /locus_tag="PWY67_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2058313.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            7462..7806
                     /locus_tag="PWY67_002081"
     CDS             7462..7806
                     /locus_tag="PWY67_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2058314.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            7877..9229
                     /locus_tag="PWY67_002082"
     CDS             7877..9229
                     /locus_tag="PWY67_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2058315.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            9289..10431
                     /locus_tag="PWY67_002083"
     CDS             9289..10431
                     /locus_tag="PWY67_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL2058316.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            10456..10710
                     /locus_tag="PWY67_002084"
     CDS             10456..10710
                     /locus_tag="PWY67_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058317.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            10966..12150
                     /locus_tag="PWY67_002085"
     CDS             10966..12150
                     /locus_tag="PWY67_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2058318.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            12147..12284
                     /locus_tag="PWY67_002086"
     CDS             12147..12284
                     /locus_tag="PWY67_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2058319.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         4168 a         2017 c         2646 g         3477 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa
     1621 aaagtataca cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt
     1681 agaatagtag agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatt
     1741 ccatacttta taaaaatctt tgataaatct attcgtagga tcgaatttaa accataattg
     1801 tgtctcatta aaaagcataa ccattactcc gatctgtttt taaaattagt ttctacctct
     1861 aatatatatc gagtttttat tttgccttca gagaatactg tttctttttt tgcagttaca
     1921 ggttgtttat tttcggaaaa agctaatata gctaaaattg aaacatccat ctggaattta
     1981 tcttttttgc tgctttgctc ataaaagtct gcatattcat cactgatatt ttttctaata
     2041 aagtcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt ctcgttgtaa
     2101 agcgatattt gaggggcaaa aagggggcaa aaatacgttt atctttgata tatctcagat
     2161 tgaacatagt gtttattata tgtcggtatt gttgatttat agctgttttg agggtattag
     2221 atacataata gaatatagtg gttttgttta cgtgcattac acggaaaagc tgctcgtatc
     2281 aaagaaatca gaagataaac acggaaaact tccttggaaa tctgaaattt gtgaaaagtc
     2341 cttgtatatc aagggctttt tcttttttct ctatcacagt tttaagccat ttttggctgt
     2401 tttggaccat ttttcaatca tattccctta aaaattgtcc tagattgtcc gacattgact
     2461 atcagtgtcg tcaaaatagg gcaaaattta gtagtcagct tgacgacaaa tataatacga
     2521 aaaataccaa aacatttacc gaatttagca acttttgaac ccaagtttct caaagggtca
     2581 gctattagca aaaaagacaa aacatgtact tttctactaa aaagttgatg tgataggctt
     2641 ctatctaaat aactattagg agtttttatc gtgaagcaaa atgatggacg tatcatttgg
     2701 aaaaatcaaa gtgagttaaa actgatttta acaattaatg aattcatcga aaaacacgga
     2761 attacatcta gccgtcaata ccagaaaaag ctatcagaaa atccaaattc tgcaccaagt
     2821 atgtggttca ttaacaaaaa atatggatca tgggagaatt tattgattag tataggacga
     2881 gaaaatactg ggtatggaaa atgggctaga atgtcagaac aagaattatt ggaaatagta
     2941 gaagccttta ttaagtgcga gaagataacc tctcaacgaa tgtacgaaca aaaatcagta
     3001 gggaagaata ttccttcact tagtaccatt aaaaagatgt taggagatat acgtccactt
     3061 tttaaagaaa aaaatgatgg atctcgattt actgatttcg aactgctgtt agaactaaaa
     3121 aatgaaataa tccgattaaa actacaggat gatttatcaa tgacaaagtt tcgaaaacta
     3181 gttcaatccc caaaactacc atcagttgat acaattatga aaagaacgaa caaaaattgg
     3241 gaggaattga tggcagagat tggatttgat tatcgaagaa tcaaaattta taagcagaga
     3301 aacaacctat ccaagacaaa gaaaactaaa tagtatcact ctaaatcaga cgttcaaaat
     3361 taaaatttgg acgtcttttt tattacccaa aaacgaaagg agatttattt tatgaaaaag
     3421 ccaagattta agaattggcg tttgctcgca acactgagtc tattatgcca aacaattggt
     3481 ggatcgcttg gcccaacgat tgcctttgct gatgaaatta ctcatccaca aacggtaact
     3541 gtggaattgg atttggctca ccagtatgct gtcgaaggta catttagcga tggtcgtccc
     3601 atgtcagaag ttaccgttcc gcactatgca gtttacaatg gtgtgaagca agatattttc
     3661 tgtattgagc caggggtgcc gatttacaat gagttcactc ctggttatga gaaaaatcca
     3721 ttgcctgata tgtcggagaa agcgaaatta gtttccgttt tatggaagga agctggtacg
     3781 gatgtggata cacatattgt ggcccagaag atgatttggc aagaagttaa tggctacgct
     3841 tcactcaatt aaacgttctg atggtagtgc agtaaatatc gcagccattg agtcaaaaat
     3901 caatcaagcc attgcggatt atcaaaagaa accaagcttc cataactcca cagtgaaaac
     3961 cgtgttaggt caatcgacta ctgtgacaga tacgaacggc ttgaatttat cggagtttga
     4021 tgaagttgtc gagaatatcg caaatattga ttatcgtgtg aacggcaatc aactagtgat
     4081 tactccaaat gccaattcaa aagagagtgg tgaattaacc ctaaagaagt cagctaatac
     4141 aggaacaccg gtagcttata aaatggctgg tcaacaaact ttgatggcgg gggcaattga
     4201 taagcccaat acctacacaa tcaaaattga tgtggaaact gagggttctt tgaagattaa
     4261 aaaagtcgat aaagaaacag gaaatgttgt accgggaacc gttttccatt tagactttgg
     4321 aaaaacatta cctgcaaaag acgtgaccac cgataaagaa ggcattgcga cattggatga
     4381 gattcctcat ggcgcgaaag taaccattac tgaaaaatca gtgccagctc cttatacgat
     4441 tgacactaca ccaatcaccg caaccattaa atctggtgag actatttctg tgacttcaaa
     4501 aaatactcgc gaaaaaggtc aaatcatttt gaataaaatg ggggtggaaa caggaactga
     4561 tctttggaac gacaaatact cattagccgg aaatacgttt gccattcgca aagacagtcc
     4621 aaccggtgaa attgtccaag aaatgaccac ggatgaaaaa ggtcatgcgg aaacaccaaa
     4681 agagattgcc aatgcgttgg aattgggaac ctattatgtg accgaaacca aagctagtaa
     4741 tggctttgtg aataccttta aaccagtaaa agtcgaattg aactatgtca atcaaaccgt
     4801 ggctcttgtt accagtaacg taaaagggca aaaccaagaa atcactggtg aaaccacttt
     4861 gacaaaagaa gacaaagata ccggtaatga aactcaaggt aaagctgagt ttaaaggagc
     4921 tgaatatact ctctttactg caaaagatgg tcaagcggtc aaatggagtg aggcttttaa
     4981 accagagtta gtgaagggaa cgaaagcttc tgatgaaacg gtgactttga ctttagatga
     5041 aaagaatcaa gttgctgtta aacacttagc gattaatgag tattactggc aagaaaccaa
     5101 agcacctgaa ggatatacct tggatgaaac aaagtaccct gtttccatca aaaaggtgga
     5161 tgataacgaa aaaaatgccg tgattacccg agatgttacg gcaaaagaac aagtcattcg
     5221 ctttggcttt gatttcttta aatttgctgg atcagctgct gggactgccg aaactggctt
     5281 caatgatctg acctttaaag tatcgccatt ggaaggaacc agtgagatta caggtgccga
     5341 agatgaagca attacagctt ataacgagca actaggtttt gatggctatg gcaagtttga
     5401 aaatcttcct tatggggatt atttacttga agaagtagag gctccagaag gatttcaaaa
     5461 aattacgcca ttagaaatcc gttctacttt taaagaaaat aaagcggatt acactaagag
     5521 tgaatacatt tttacgatta ccgaaaaagg acaaaaacaa ccaattaaaa cggtgaccgt
     5581 tccttacgag aaactgacga ataaagcatt ttctgttagc ttgaatcgtt tgatgctcta
     5641 tgatttacct gaagaagaag atagcttaac ttctcttggg acttggaaag acggaggcaa
     5701 agaattgaca gaccttgatt ctaccgagct agttgataaa ttgagctata acttgcatga
     5761 aatcaaagaa gactggtatg tggtagcgca agccattgat gtggaagcga caaaagctgc
     5821 ccaagaaaaa gatgaaaaag ccaaaccagt ggtaattgcc gaaacaaccg caacgttggc
     5881 gaataaagag aaaacaggaa cttggaaaat tttgcataaa ttaaccgcag aacaagtttt
     5941 ggataaaacc atcgtcttat tcaactatgt gtatgaaaac aagaaaccct atgaagcagg
     6001 tgatgatcca gtagcgaaag atgctagctt gaacaatcaa gcccaaactg tcaattgtac
     6061 ggtggaacgc catgtttcca tccaaacaaa agcccaccta gaagatggtt cgcaaacctt
     6121 tactcatggg gatgtggtgg atatgttcga tgatgtgtcg attacccatg atgtactgga
     6181 tggctcaaaa gaagcttttg aaacaattct gtatgcatta cttccggata atacgaccaa
     6241 agaaatttgg aaatctggca aaattgatta tgaagtgaat gacaaagaat tcactaaaac
     6301 cgtacttgcg gaaaaagtgg acacaagtaa atatccagaa ggtacaagtt ttaccttcaa
     6361 agaaattaac tatggcaaag acggaaacat caatgggaaa cacaatgagg atctaaaaga
     6421 aaaaactcaa actttaacac ctaaaaaagt tccgacaaca ccaagtgcac cagaacaacc
     6481 ggaaatacca actgttccaa gtgactctca agaatctagt cctacagtaa agacattccc
     6541 acaaacaggg gagaaaaatt ctaatgttct gctgtttatt gggtttacct tgatctttgc
     6601 gactgctggg tattatttct ggaatcgccg aaactaaggt gatgtgatga aacaactaaa
     6661 taggaaaaac caacgtgcgc gaccgcctcc taaaaaaagt ggtcgcgcta tttttgtatc
     6721 caaaactaaa aatgaaaaga ggaaaagaag atggaattga aatttgttgt gccagatatg
     6781 gccgaaacct ttggaaagat aaggtatgcc ggcgaaggcg aagttttaac tgaaggatat
     6841 gggcggaata ctacagtaat tggtcgtagt tatcatttgt attccagtaa gcaacgagcc
     6901 gatgatattg aagtggtggt agctgcggaa gccggcgaaa aggattttga tcaagatcaa
     6961 ccactaaaag ccgtgaatcc ccatttggtt gccaaaggct atgagattga gaatcgtggg
     7021 tttaccgact acgtgttgta tgtcgatgat ttagtgaaag cataggagga aaagaagatg
     7081 agattaacag aaggtattgt tgtagattca ggactaacgt ttgggaaatt acgattttcc
     7141 gcattacgcc gagaagtacg gaagcaaaat gaggatggaa cggttagtaa cgaggtaaaa
     7201 gaacggactt ataatttgaa atcctctgcg caaggtcgaa tgatccaagt tagtatccca
     7261 gcaaatgtac ccttacgcga gtttgcctat gacgcagagg tagagttggt caatccaatc
     7321 atggatacgg tcgccaatta tgttttccga gaaggaacta ctgtcaattg gttcattaag
     7381 gccgatgatt tagtcttgaa acgacaacca gatcagggaa attctacaaa ccaaaatgaa
     7441 ggaaagaaat aggtgaagcg tatggaaatt aatgttgaac atattttaga ttgtctcgat
     7501 caatatggaa aaggtgagct aacggaggaa caactcacta aagcattaac ctatgatgaa
     7561 aaaatgtttc tgattatgca tcaagggtta ctggaagtag gtaacgatgc cgaagaagat
     7621 tttgatgtac tgaattggtt aggggaagaa gagtccttct ttatggttat cgaagtaaat
     7681 gaaagtctat gtcgagaagc cgaaaccgta ttggaagaaa ttggtgtgga aatgccggat
     7741 gccattgaag tttttttaaa gcaactagtg gaaacgaaac aactccctat agcagtaaat
     7801 gactagcata actgcttgaa attggatcaa tgaaatccta tgaagctttt ttcgattgga
     7861 ggtggaaacc ataaaaatgt acaaagggca tcgtatacgg gcaggagatc agcacttggt
     7921 ttatcacttt gttcttggct ggctactcgc tctgttcatc ggttggatga gcgtctttta
     7981 ttttcaagag cttagacaat ttgatatttc taagctatca ctttctacta tagaaatcgt
     8041 tcgttctata aaagatttaa tctatttgct aggaagtctc gttctttcag gaagcacgat
     8101 gttgctttat atacactttt ttcaggatca ttggcgcagt ttgtggcatc gacaaaagct
     8161 agcccggatg attctagaaa atcattggta cgaagtaaaa caaactcaga gtgaaggatt
     8221 tttcaaagac ctcaatagta gtcgaaccaa agagacaatc agttacttcc ccaaaatcta
     8281 ttaccgtatg aaggatggtt tactttctat tcgcgttcaa atttcactgg gaaaatatca
     8341 agatcagctt ttaaagctgg aaaagaagtt agaaagtggt ctatattgtg aactagtaga
     8401 aaaggaactc aaagactcct atgtggagta cactttactg tatgatataa ttgccaatcg
     8461 aattgggata gacgaagtag tggcagaaag tggggctttg cgattgatga aaaatcaagt
     8521 atgggcttat gattctttac ctcatatgtt aatcgctggc ggcacaggtg gtgggaagac
     8581 ttacttttta ctcactatca tcgaggcttt attaaaatct gatgccgagc tttttgttct
     8641 tgatcccaaa aatgcggact tagccgattt aggtacggta atgcctcatg tctattccca
     8701 aaaggaggaa atttctgctt gtgtggaaga tttctatgag cgcatgatga ctcgtagcaa
     8761 ggcaatgaaa gaaatgccca actacaaaac aggagagaat tatgcgtttc ttggacttcc
     8821 accaaacttt ttaatctttg atgaatacgt ggtttatatg gaaatgttaa cgacgaagga
     8881 aagtgcggtg attttgaata agctcaaaca aatcgtgatg ttaggtcgtc aatctggttt
     8941 ctttctgatt ctagcttgtc aaagaccgga tgcgaaatat ttaggagacg ggattcgcga
     9001 tcaatttaat ttccgggtgg ctttgggtcg tatgagtgaa ctgggatatt ccatgatgtt
     9061 tggcgaggtt gataaaaaat tctttatgaa gcgtatcaaa ggtcgaggct atgtagatac
     9121 gggagggagt gtgattagtg agttttatac gccacttgta ccaaaaggat atgatttctt
     9181 aagggaaatt agcgacattg gtggattaaa cgttaatacg gaaggataaa ataatagtat
     9241 gtagcctagg agtgtcaaga atcctgggct ttttttacga ggtgatttat gacattttta
     9301 gatttattcg ctgggattgg tggcttttgc ttaggtatgg aacaagcggg tcaccagtgt
     9361 atcggctttt gtgaaattga tgattttgct cgccagagtt ataaggcaat ccatgataca
     9421 agtaaggagg tggaaatgca tgacatcaca agtgtatcag acgagtttat tcaatctctc
     9481 ggaccagtgg atatcctttg cggtggattt ccgtgccaag ctttttcaat tgcgggaaag
     9541 cggcaaggat tttccgatac tcgaggtact ttattctttg aaatcgctcg gttcgccgct
     9601 attctccaac ctaagttttt attccttgag aacgtccggg gattactcaa tcacgaagga
     9661 ggggctacgt tcgagacgat cctccgaacg ctggatggat tggggtatga tgtggaatgg
     9721 caagtgctta actcaaaggc ctacgttccc caaaaccgtg agcggatctt ccttatcgga
     9781 cattctcgag acgcatgtac cgaacaagta tttcctatca ttggatcgtc tccaacatct
     9841 gatcaaaaca tcagaaactt attaaatatc aacccttcca atcgaggaat ggggggacaa
     9901 gtctatgggt cagatgacgt agcgcctacc ttgaccggtg atgaaggaat caaaatcgca
     9961 ttgccggtag atgatggaat ttctgtagcg ggtatgttac tgggaaattt tgaacaaggc
    10021 aatcgggttt atgagataac tggaacagcg cctactttgt caacgaaaca aggcggaacg
    10081 aagatcatgc ttcggaaaaa cgaaacagaa tatcaagagg tgaagccagg gaacagtgtg
    10141 aacctggctt ttcctaattc cacaagtcgt cgaggccgtc tgggaaaaca aagtgtgcat
    10201 accttattaa caggagacca acaagcagtc gtcacggatc agtatcaaat tcgaaagtta
    10261 acacctaggg agtgctggcg gcttcagggc tttccggact gggcttttga tcgagcttct
    10321 caagtcaatt cagatagtca gttgtataag caggctggaa attctgtaac agttccggtc
    10381 atttttgata ttgctagaag attaaaggag gtaaaaacag atgatttatg atgagttgat
    10441 tggggaatct actgggtgat tggcaagatt cagtcagatc cggaattgga agaagaactg
    10501 taccggctca attttgatat tcgcaaaaac ggggtgaaag tccctgatga tccgtatgta
    10561 attgatgagg aaacggatgc tcgaatggag ctgaatcaag tcatcagtga attggaacga
    10621 attgcggact ttgctaaaga acctgatatc cgccaatact tgtttgagat aaaagcagag
    10681 ttggaaattg agggtattac cgcggagtaa cggccaaata aaccactgat aggcactgac
    10741 cgcgggggat ttgcgaagca gaaatcctcc gtggtgctgt gcctgtttat ggacggcgtt
    10801 agccggccat gatgtctcac cccccgtatc taacaggggg gtacaaaaac acaaaaaata
    10861 agcaagcaac gataaggaat ggctgtccta tcaaggataa taagcaattt gtgtagatgt
    10921 taactgattt gaaaaagtta acaccttaaa tttggaggtg agaacttggc acaaaggaat
    10981 ttagattatc ggctattaaa agaccggcgt aatgaatatg gcgtttcaca aaataaatta
    11041 gctacgactt gtggacttag tcgcccgtac ttaaatcaga tagaaaatgg tggcgtaacc
    11101 gcatcgacaa aaaccatgag gaaaattttt gaccaactgg aaagtttcaa tcccgagtta
    11161 cctttaacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga tgcgaggaaa
    11221 atcattcaag agattctcca tttgaaattt gattatatgc tccacgaaga ttacgccttt
    11281 tactcctatc aagaacaata tgttatggga gatattgtgg tgatgttgtc acatgaagaa
    11341 gataaaggcg tccttttaga attaaagggt cgtggttgtc ggcagtttga aactttttta
    11401 ctcgcccaaa agcgtagctg gtatgacttt ttcgaagatt gtctaaaagc cggtggcgtg
    11461 atgaaacgct tagacctggc aatcaatgat cgagttggcc tgttagatat tcctgattta
    11521 acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtaccttcaa aagctatcgt
    11581 tccggggaac tgttgaaacc tgatgataaa gatggtatgg gcaatacttt gtatattggc
    11641 agtcttaaga gtgaggtcta tttctgtttg tacgaaaaag actatgagca atatatcaag
    11701 ttagggattc ctttagacca gaccgaaacg aaaaatcgtt ttgaaatccg actgaaaaat
    11761 gatcgagctt atcatgcaat tcaagatttg ttaaaaggtc gcagtattga aagtaccaca
    11821 ttttccatta ttaaccggta cttgcgattt gcggataagg tggaaggcaa aaggcgaact
    11881 aactggccct tgaatgaaca atgggggcgc ttcattggac ggaatcagaa ggaaattcaa
    11941 ttaacttctg aaccgaagcc ttataccatt gaacgaacgt tgaactggct tggacgacaa
    12001 gtcgccccta cgtggaagat ggccaaggag ttggatcggt tgaatcagac aacgtacatt
    12061 caggatatgg taaataatac tcgactatca gaccggcaca aaaagatttt ggagcaacaa
    12121 agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtgatggaa attgtgaaag
    12181 atattttatt ggtgtttggt ggcagtatgc ttggtgttac tgtgatgtgc ttgatgcatg
    12241 caagtgcagc ggcggaccga gcaatggaaa agaaaggaaa gtgatgaaat gaatttcggt
    12301 caaaattt
//
LOCUS       DANKAZ010000077        11869 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000077 DANKAZ010000000
VERSION     DANKAZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            37..2055
                     /locus_tag="PWY67_002087"
     CDS             37..2055
                     /locus_tag="PWY67_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2058320.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2125..2331
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2482..4896
                     /locus_tag="PWY67_002088"
     CDS             2482..4896
                     /locus_tag="PWY67_002088"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL2058321.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5244..6797
                     /locus_tag="PWY67_002089"
     CDS             5244..6797
                     /locus_tag="PWY67_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2058322.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7178..8962)
                     /locus_tag="PWY67_002090"
     CDS             complement(7178..8962)
                     /locus_tag="PWY67_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2058323.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8977..10698)
                     /locus_tag="PWY67_002091"
     CDS             complement(8977..10698)
                     /locus_tag="PWY67_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2058324.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10798..11559
                     /locus_tag="PWY67_002092"
     CDS             10798..11559
                     /locus_tag="PWY67_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2058325.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3879 a         2152 c         2415 g         3423 t
ORIGIN      
        1 attaataata gataaattga agttggagga tagaagatgg aaaacattgc aagaaaagaa
       61 cggagacgtt tagaacagac caaacgtttt cgaaaagcaa aacgaggggc agcaatcgtg
      121 ggaactgcga tggtaggctg ttctgtcgct gccccattag tacagccggt tcctgttcaa
      181 gcggatgaaa cacctgttca gttcggcgca agaatcaata cagcggcatt tatcacagaa
      241 attgctactt atgctcaacc aatcgctcaa gccaacgatc tatatgcatc agtgatgatc
      301 gcacaagcag ttgtagaaag cggttggggc agtagtgcgt tatctcaagc accttattat
      361 aatctttttg ggataaaagg cagctatcaa ggacaaaccg tctacatgga tacgcttgaa
      421 tttttaaata atcaatgggt aaataaaaag gagcctttcc gacaatatcc ttcttttgct
      481 gaatctttca atgacaatgc ctatgtactt agaaatactt catttggcaa cggttattac
      541 tatgccggta cttggaagag caacacgaaa tcctatacag atgcgacagc ttgtttgaca
      601 gggcgttatg cgactgatcc tggttacgca ggcaaattaa ataatatcat cgcaacttac
      661 ggattgaccc aatatgatac accagctagc ggaaacgcag gcggtggagc gactacaggt
      721 ggcaataata ctggaagtgc aggtagtact ggcggttcaa gcaattctgg taatacaaac
      781 tcaggttcag caacttcgac cacttatact gtccaatctg gtgattctgt ttggggaatc
      841 tcaaataaat tcggtatcac aatggatcaa ttgatccaat ggaacaacat ccaaaacaat
      901 ttcatttatc caggacaaaa actgacaatc aaaggtagcc aggaaaatgg ctcttcgacg
      961 aataattccg gtaacaatac caattcttca ggaaatgctg gtacatcaaa cggaggacaa
     1021 acaactggtg cgaaatacac tgttcaatct ggtgattctg tttggaagat ctcaaatgat
     1081 cacggaatta cgatggctca attgattgaa tggaacaata tcaagaacaa tttcgtttat
     1141 ccaggacaac agctaatcgt atcaaatggc ggttcttcta atgctggagc aaatactggc
     1201 tcaaatactg ggaatacaag tggctctagt tcgtccagct caagttcttc taatacagct
     1261 tcaggaacga aatatacagt taaagccgga gactctgttt ggagcgtctc aaataaatac
     1321 ggaattacga tggctcagct aatccaatgg aacaatatcc aaaataactt tatctaccca
     1381 ggacaacaac tgactgtttc aaacagtgga tcaacaaaca gcacgtcagg taatacaggt
     1441 tcgtcaacca atacaaataa cagcagttca gttaatactg gcagcagttc gtctcaaggc
     1501 acttataccg tgaaagcagg tgaatctgtt tggagcgtgg caaataaaaa cggcatcacg
     1561 atggatcagt taatcgaatg gaataatatc aaaaacaact tcatttatcc aggacagcaa
     1621 ttgatcgtta aaggtggaac ttcggcaaat acaaatacag gttcaactac ttctgcagcg
     1681 aaaccaaata caccgaatac gtctgctacg acaagtacat catccactgg taatacgatg
     1741 tataccgtga aagcgggcga gtcagtttgg agcgtagcga acaaacatca tatcacgatg
     1801 gatcagttaa tcgaatggaa taatatcaaa aataatttca tctatccagg acagcaagtg
     1861 attgtgaaga aaggttcatc tcaatcaaca aaccaacaat cagttactgg ttcgaaaacc
     1921 tatactgtga aatctggaga atctgtttgg agtgttgctg acagtcatgg aattacaatg
     1981 gctcaactaa tcgaatggaa caatattaag aacaacttta tttatccagg tcaaacgttg
     2041 attgtagcta aataaaatag ctgttcgaag aaataacgat tgaattccac aaaaatattt
     2101 gttactataa ggctacttaa atatgacaac acttgaaaag gaagtagtaa aaagactgaa
     2161 gtttcagaga gcgcatggct ggtgagaatg tgcacggatg cttttgaact cgcctttgag
     2221 ttgcccttgc tgaatacccg agtaagcaag cggcggtgac ggccgttaca acgttcgagg
     2281 ccttttcact aaggtaaaat taggtggtac cgcgtagatt acgtcctata aaaagagcaa
     2341 tctttttata ggattttttt atttcttttg tgaagtttga gaaaacaaag caaaaggaaa
     2401 taaaaataaa cagattctgc cggttttttc gttatattta taatagaaac aagtgttttc
     2461 aatagaacag gaggacagca aatgagttac aatcataaag aaattgagaa aaaatggcaa
     2521 aaatactggg caaaaacgaa tactttcaac acccatgatg atcctgaaaa gccaaaattt
     2581 tacgcactgg atatgtttcc ctatccatcc ggtcaagggc tacatgtagg gcatcctgaa
     2641 ggttatacgg caacggatat cctttcacgt gtgaaaagaa gtcaaggata caacgtgctg
     2701 catccaatgg ggtgggatgc attcggtctg cctgctgaac aatatgcgct agatacagga
     2761 aacgatccag ctgagtttac ccaaaaaaat atcgaaacat tccgacgcca aatcaattct
     2821 ttaggattta gttatgactg gaatcgcgaa gtgaatacaa ctgatccgga gtattataaa
     2881 tggacacaat ggatctttac gaagctttat gaaaaaggtc tagcttatga agcagaagta
     2941 gcggtaaact gggtacctga attaggaaca gttatttcaa atgaagaagt gatcgatggg
     3001 aaaagtgaac gtggtggcta tgatgtagtc agaaaaccaa tgcgccaatg gatgctgaaa
     3061 atcactgctt atgctgatcg cttattggat gatctagaat tagttgattg gccagaaagc
     3121 attaaagaaa tgcaaagaaa ttggatcggt cgttcagtag gagcaaatat tgaatttaaa
     3181 gtggctggta cggataaatc ctataccgta tttacgacac gaccagatac tctattcggt
     3241 gcaacttact cggttcttgc acctgaacta gatcttgtta gagaaattac gacccctgaa
     3301 caaaaagctg ctgtagaagc ttatatcgaa gagacagcta aaaaatcaga tctaaagaga
     3361 acggatcttg caaaagaaaa aacaggagta tttactggtg cttatgcaat caatccagtt
     3421 aatggcaagg aaatcccaat ctggatcgcc gattatgtat tatcttcata cggcacagga
     3481 gccatcatgg ctgttccagc acatgatgaa cgtgactttg aattcgctca aactttcgga
     3541 ctagaaattc ttccagttat cgaaggtgga gatgttgaaa aagctgctta cacagaagat
     3601 ggtacgcaca tcaactctga atttctagac ggcatgaaca aacaagaagc aatcgataaa
     3661 atgaatgaat ggctagaaga aaatggcgta gggaaaaaag aagtcagcta tcgcttacgt
     3721 gactggctat tctcccgtca acgttactgg ggtgaaccaa tcccaatcat ccattgggag
     3781 gacggaacag tcacagctgt cccagaagaa gagctaccat tacggttgcc taagacaaaa
     3841 aacatcaagc caagcggtac tggagaatct ccattagcaa atatcgagga gtgggtcaat
     3901 gtcgttgatc ctgtaacagg taaaaaaggc cgtcgcgaaa cgaatacaat gccacaatgg
     3961 gcaggaagct catggtatta cctgcgttat atcgaccctc ataacaaaaa agaactagca
     4021 gattatgaaa agttgaagcg ttggctacca gttgacatct acattggtgg agcagaacat
     4081 gcggtacttc atttactata tgcacgattc tggcataaat tcttatacga tatcggtgtt
     4141 gttcctacaa aagaaccatt ccaaaaacta tacaatcaag gaatgatctt aggcgaaaac
     4201 aacgaaaaaa tgtcaaaatc tcgtggtaat gtagtcaatc ctgatgatgt ggtcgaaact
     4261 tacggtgcag atacactgcg catgtacgaa atgttcatgg ggccattaga tgcttcgatt
     4321 gcttggagcg aaaatggctt agaaggaagc cgtaaattct tggatcgtgt atggcgtttg
     4381 atcgttgatg agaacaataa aatgcgtgac cgtatcacta cattgaatga tggcaagctt
     4441 gataaagtgt atcatcaaac agtgaaaaaa gtgacagaag actatgaaaa tcttcatttc
     4501 aatacagcaa tctcccaatt gatggtcttt atcaatgaag cctataaagt ggacgctctg
     4561 ccttatgaat atatcgaagg atttgttcaa ctactcgcac cgattgctcc tcacatgggt
     4621 gaagaactat gggcaatcct tggaaatgat ggtggtattt cttacgcacc atggccaaca
     4681 tacgatgaag cagcacttgt tgaagacgaa gtagaagttg ttttccaagt aaacggtaaa
     4741 gttcgtgcga aaagcaatgt tccccgagac ttaggaaaag atgagctaga aaaagcagca
     4801 ttagccaatg aaatagtgca agaatatatt gaaggcaaaa cagtcagaaa agttattgcg
     4861 gtaccaaaca agttagtcaa tatcgttgct aattaattgg ctctttgtca ataaggactg
     4921 atgagttgtg caaaatcaaa tctgggccag aatgaacttc attctgtctc agattttttg
     4981 ttatttcaaa ttgattaatt gattgatcaa aaaggttttg attttcatgt tctcaaacac
     5041 gttcaatgtc tttttcttct gaatggtctt gtaagagatc tctgtaatca tcaaattttt
     5101 gtctattaat cttagtattt tttgatgtat aaactacctg aaattgagag tacataccaa
     5161 attcataaat tttttattag tgggactagt gattgtatgg attttgacgc ataatgaatg
     5221 aagtatgaga aaaggagaga caaatggatc aagaactatt tattatcgaa gtattatcag
     5281 ctaaacaaaa aattatcgag aaagcgaaaa aaagagcgaa aagaataatg ttcaagcttt
     5341 taagcttagt atttttctct ggtttgcttt ttttcggagc tgtatcgttc ctatatcgcc
     5401 atatgagtgc agtagtccgt tcagctgaag ggatgcaaaa attaaataat ttgtatcact
     5461 tttcgtgggg agttggcgga ataacaattg ttgtgcttat tgtttttctt ctacttattt
     5521 ggctccgtta ccatcaattg tcgcgtcatt tcgatcaatg gcttagtcaa agagcacaac
     5581 agtttttaca taataaacca acttctcaag atgatgacta tctttatttt gagaagactt
     5641 cgacgagtca agcttttcgg atacaaaaaa gtgattgcca gttattgctg agatcacttg
     5701 atgatttacc tatctactta ggcgaaagca aatcaaaatt cggaatgaac acattccata
     5761 tttttttact agaaaacgaa cccagatggg aaaaaagcca gtcttcgcct ggtggatacc
     5821 tgacaactaa gatgagtaca ttagtcactg gttttttcct cattctttta gctggaacaa
     5881 tctattatgg gagatacact cagccaaaaa tgtcttctca tataaatgct gcttctcaca
     5941 tgacaatgaa ctcaactaac gatacgcata tacaaaagac aaatgaaggg ttgttagctg
     6001 atcctaagca aaaggtaaat ttgacaacac aaaagataaa tgatctcaaa cttgatacag
     6061 agacgcactc tctctatatg acaacagata gtggtgctaa ttgggtattt gtgccgattg
     6121 attcgcaatg gttgcgtttt ggcgaataca ccctgacaac ggggctgatt cctgaaggtt
     6181 attggatgga caacacatat gatatttccg ccgatttctc atggtttatc tattcttctg
     6241 gggatgatgt acgtctgctg acttcaagag ataatggtaa aacatggcaa acgtctatta
     6301 tcacccaatc agttcagccg atacgttatc gaaaagcggt attttatggc aacgatggtc
     6361 tatttatctg tacatcagcc agtgggatgt ctgcagaaac tttgtacatt tacactacta
     6421 tggatggcgg aatcacttgg ataaaaagtg gaagtacaat gatcaatcag ccagtacaaa
     6481 atgtcagctt cgttacaact tctttaggtt ttgtttcgac acgtgaaagt ttgttttata
     6541 caaacaacgg aggacgctca ttcaaagaat cagttctgtc gattccggaa aactatcaga
     6601 caggggggat agatcttttt caatcaccaa atgaagttac tcaaataagc gcgaatacgt
     6661 tagagacaaa attttatttg ttgaaaaaag gaccaatcga tgcagggaaa atgtttgcct
     6721 gcctcttccg ttcaactgat aacggggaaa catggcaatt cgttcagcaa ctatcgcaag
     6781 tagaacaagg agattaaaga agagagattg tgagcctgac gttcagcacc gagtcacaag
     6841 aaacaaattc aaaaaacagc ttctcatatt tgttgaatag tgtaactcgt gattcggagt
     6901 tgacaagagc aacaagaagc gattttgaag tatagcttct cgtattttac aagatcgtgt
     6961 cttattgccg ataggttgtc tcttgaacac cgtttattcg taaaaaggga ttgtgacaag
     7021 tcttttgtca ctacttaccg aacgaaactg aaggagctat ccaaaaagta tcattagtat
     7081 tattctttgt gtttctagtg gcaccactag tacatttaaa aaagctatcg atcatggact
     7141 aatcatacat gatcgatagc tttttagata atgtttgtta cgctatctga aattgactat
     7201 tataaagact ggcataaagc gttggtgatt ggagtagttc agaatgtgtt cctttttcga
     7261 cgatatcgcc attcttcatt acaaggatca tgtctgcatt ttcaatcgtt gacaaacggt
     7321 gtgcaatcac aaaacttgtc ctatcttttg tcacagcttc cattgcttgc tgaatcaatg
     7381 cttctgttcg tgtatctacg ctagaagttg cttcatctaa aataacgaca ggaggattcg
     7441 ctagaatgac tcgagcaatt gtcaataatt gttgctgtcc ttgagataac gtgccgtttt
     7501 cgctagaaat gatcgtatca tatccgtctg gcagtgttcg aataaagtga tcacattgtg
     7561 cgatttcagc cgcttcgata atttgttctc ttgtggcatc cattttcccg taggcgatat
     7621 tatcagctac cgttccttca aacagccagg tattttgtaa aaccatccca aaagaacgac
     7681 gtagttctcc acgtgaaagc ttggtaatat caagaccgtc gaattcgatc tttcctttat
     7741 tcagttcata aaaacgcatc agcagattga ctaaagtcgt ttttcctgca ccagttggac
     7801 caacgatcgc taccgtttgt tttggcttta ctgaaaaatc gacttgattc atcaatattt
     7861 tatctgatgt gtaaccaaac tgtacatttt tgaaagctat ttttcctttc ggttgttcta
     7921 aaagtcttgc atctggaagg tctggaattt catctgtttc atctaatatc tcaaatatcc
     7981 tatcgattgc tgccattgcg gattggatcg agttgatcac ataagaagcg gtagaaattg
     8041 gttcagaaat ctgattgatg tactgaagat aagcttgtaa gaagccgatc gtcacaccac
     8101 ctgatagaac aagcatagca ccgaataccg cacttatgat gaaggcgagt tgattaatga
     8161 aacgaatggc cggatagata gcacagttca taaattgagc acgtttaaaa gcgttgtatt
     8221 gctttttatt tacttcgtgt atagcagctt ctgcatgagc ttgctgattg aatgttttga
     8281 ccactagatt accagctaag atttcttcca ttcgattatt taaaacaccc agttcatact
     8341 gattctgttc agaaatctgt ttgtttttgt tcgcaatctt agttgtaaca attgaactgc
     8401 caacgatcag caataagacg agcaaagtaa gcttgctttc gatataaaac agcatcagac
     8461 cagcagcagc tattgttacg acagaagtaa aaaattgatt gatacctgtc aataacactt
     8521 gagaaagttg attgagaccg gtagttgtcc gactaatgat gtcacctact tgatgattgt
     8581 caaaaaacga catcggtaat gttttgaatt tttttgtgat ttcttttcgc accctcaatg
     8641 tgatcttttc gcttaacgct gccattgtcc gttcctgaat aaatgaagtg atgctgctta
     8701 agactgaaca gccgatcagc aataagacag ggaacaacaa tgcttgtttg atatcactga
     8761 aagaaagctg tccaatacct gtttttctta tttctgataa gagatcatcg atcccgattc
     8821 ccatgatcaa gggcatggct acgactaata ggttcccgaa taaactgcat agaaacaatc
     8881 cgtaaaaata tttatgttca ggtttgatca ttttccaaaa acgtatcaaa gcacgcgtgt
     8941 tcaacgtttt tttcttttcc atcgtatccc ttctttctat attccttgtg attttgcaaa
     9001 ctcctgataa gtttggcaat tagacagtag ttcttcatga gtaccttgtc caacgatttt
     9061 tccttcatct aaaacaatga tgttatctgc ttgttggatt gtactgaggc gttgagcaac
     9121 aatcacgaga gttttctccg acatgtgttt atgaagggct gctcttaatg ctgcatctgt
     9181 tttatagtct aaagcggaga aactatcatc gaatacatag atctctgctg gtttgatcaa
     9241 cgctcgagca atacatatcc gctgtttctg gccccctgaa taattagaac cgccttgagc
     9301 aacaaagctg tccaatccgt caggaagggt gtccactagt tcttctaatt gagcgatctt
     9361 gatcgcttta gacaactctt ctgttgaggc atattcattt cccatctgca agttgcttaa
     9421 aattgtcccg ctgaataaga aagccttctg agggacataa ctgatgtggg agcggatctg
     9481 ttcttgagtc atttgagtaa gaggaatacc accaagggtt acttttccta tcgtagggtc
     9541 actaagtctc aagagaagtt tcgccaaact actttttcca gatccggtcc cgccaacgat
     9601 cgctgttgtt tttcctttag gtattgtgaa atcaatatct tctagaactg gatcagagat
     9661 ttcagtatat ttaaatgtga catgttcaaa agcagctgca tttgtctgat caaaggttgg
     9721 ttcagctact gttttgatcg tctcttcctc aatttcgatc tctgcttgca gtatttcttc
     9781 tatccgtcgt aatgaggcaa acgatttagg gataatgacc aataccatag ctgcgatcat
     9841 caagtaagca agtgtcagca tagagtattc gactacagca gtcacgacac cgatttgtaa
     9901 tgctccttct gctacaagat ttccgccaaa ccacaaaata gaagcataaa cgattcccat
     9961 gagcaaaaaa gcaattggag tgacccaagc aaatattcgg ttcactttga tcatttgatt
    10021 agcatagtca gcaaagctct tattcgttcg cttttcttca tatgattgat tatcaaaggc
    10081 acggatcata ttaatcccag taaagaattc tctcaatgtg atggtgatcc gatccatttt
    10141 tggctggata ctcaaagata aaggtgcccc tcgtttcatc agtcgataaa caatccatac
    10201 ataaaaaaca acagagatca atgtaaccca tgttagtaat agagaataac gaaacatcat
    10261 gagaatggtg aaaacgcaaa taaccggtgc tggtaagatc atttggcaaa ataaaacaat
    10321 catttgctgg acattatcga catcattcgt cattcttgtc aaaagagatc cagaactaaa
    10381 acgactgata tccttcattg acagctgttg gaatttacgg aaatatttct cccgcgtttg
    10441 gaatccaaac ctagcggaag ccaatgctga aaagtaacta gaggcaactg cagcgattgt
    10501 tcctagaaac gctacaagca gcatttcaac tccaattttt tttataactg aggcttcgcc
    10561 acttgctata cctgtgtcga tcagctttgc tactaatttt ggtacaccta atgtaccggc
    10621 gatttctaaa cagatcatca caaaggtaag gagagcgagt cctttgtttt tctgtaagaa
    10681 tgtttttaag atagacatat tattcctcct tttttcctga tacttttcga ttctatacct
    10741 tcaagtaact tgaaggtcaa tagaaaaaag aataaactga aaaagaggtg agaaaaaatg
    10801 aaaaaaatgt atacgattgg ccaagtatcg gaactatttg atttgccaaa atcaacgatc
    10861 cgctattggg atgaacaagg attaatccgt tcaagtagac aagaagagaa tgattatcga
    10921 ttgtttgata tcgatgatat ttttatgctt tatgatatcg ttttttatag aaaattagat
    10981 ataccgatca aacagatgaa gaatttatat ggaaaaacat tgacggagtt atatgagaca
    11041 cttgatgaaa cagaaagaag aatccataaa gaactggtgg tgatgaaaca aaaacaaaaa
    11101 gagataaggg agcaaaaaaa acagttgaaa ttgatgatcg atacaaacga ggaagaattt
    11161 cctgtagaag agatcccttt tgactgtatg atctcaacgg agtttgaaga tattgtggaa
    11221 atcaaaaaat ttctacctaa ctattcaagc tttgggatga tgagtatgcc agcttccagt
    11281 tcacagacga tgtatggatt ttttatcgac cctagtgaag tacatttatt tactcaagac
    11341 gtgatttggg agaaaaaaga tacggctgtt tatcgtcgat tcttattgaa atccgagatg
    11401 aaccatgcgg aacgtaataa tattttagaa atcagagaaa gaatgtatga aaaaggatgg
    11461 aaaacaggag aagtgatcgg ccagtatcta ttgacgaata cggatgaaaa caatatcaga
    11521 actgaatatt atcatgcatg gatagaaatg aaaaaatagg aaatatgtgt ttaagataac
    11581 tcgaaaatga tggttgtttt agttataaag aaaaattttc tggttcgatt acgggtttaa
    11641 tgtgggggat tattagtcgt ggatagatag aattttagtt ttttagagga aatatgtctt
    11701 taaaaatgga atggttgcaa ttcttagcga tatatacgat gtaattgtcc tcttaaaaaa
    11761 gtatggaaaa ataattgtat tgggtgttat gtataattca tggtgaactg agactaatga
    11821 gaagaaaaat tcatattgtt atgagtgaga ttgtatcaga tgagaatga
//
LOCUS       DANKAZ010000078        11668 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000078 DANKAZ010000000
VERSION     DANKAZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(38..217)
                     /locus_tag="PWY67_002093"
     CDS             complement(38..217)
                     /locus_tag="PWY67_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058326.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(598..1305)
                     /locus_tag="PWY67_002094"
     CDS             complement(598..1305)
                     /locus_tag="PWY67_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058327.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1298..1735)
                     /locus_tag="PWY67_002095"
     CDS             complement(1298..1735)
                     /locus_tag="PWY67_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058328.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1750..2001)
                     /locus_tag="PWY67_002096"
     CDS             complement(1750..2001)
                     /locus_tag="PWY67_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058329.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2001..2207)
                     /locus_tag="PWY67_002097"
     CDS             complement(2001..2207)
                     /locus_tag="PWY67_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL2058330.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2644..2904)
                     /locus_tag="PWY67_002098"
     CDS             complement(2644..2904)
                     /locus_tag="PWY67_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058331.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2979..3182
                     /locus_tag="PWY67_002099"
     CDS             2979..3182
                     /locus_tag="PWY67_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDL2058332.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3323..3808)
                     /gene="ssb"
                     /locus_tag="PWY67_002100"
     CDS             complement(3323..3808)
                     /gene="ssb"
                     /locus_tag="PWY67_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2058333.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3811..4710)
                     /locus_tag="PWY67_002101"
     CDS             complement(3811..4710)
                     /locus_tag="PWY67_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL2058334.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4723..5316)
                     /locus_tag="PWY67_002102"
     CDS             complement(4723..5316)
                     /locus_tag="PWY67_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL2058335.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5392..5541)
                     /locus_tag="PWY67_002103"
     CDS             complement(5392..5541)
                     /locus_tag="PWY67_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058336.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5557..7761)
                     /locus_tag="PWY67_002104"
     CDS             complement(5557..7761)
                     /locus_tag="PWY67_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL2058337.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7772..9232)
                     /locus_tag="PWY67_002105"
     CDS             complement(7772..9232)
                     /locus_tag="PWY67_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL2058338.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9293..11665)
                     /locus_tag="PWY67_002106"
     CDS             complement(9293..11665)
                     /locus_tag="PWY67_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058339.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1958 g         4386 t
ORIGIN      
        1 gtgctatcac ttttagtttt taggataaaa agtgacgtca ctttttgagt gctggtacct
       61 caaatcctgc tttttcttgg gcaatcaata aatctaacgc aatacacata atcctggatt
      121 cacttttgcc gtacttctgc tttagttgct gcaattcttt ttgtctcttt tcatccaacg
      181 tcacgggtaa ccgtttattt cctattgcaa ttggcattag tactctctcc tttcatcgaa
      241 ataaccctat taaaaatatg gctgcaatca acttgaatat tagactcaca agggttaaca
      301 caaagcgcca aaagatcaaa caaaatacca gtattcctaa gaatactacg aagtttaaat
      361 gtagagctgc accatgacgc ataagtatct cgccaacaaa cagtacgaac aatagaggaa
      421 tgattttatt catatggatc tccttctttc tcttaggtta tcgggctttg cccgcttatt
      481 tcgcaccacc taagcggcgg gggcggttgg atcgattggt gcgaaataag aaccattccc
      541 ttaatttcaa ggttttttgt tcttatttca cacatcctca cttttcggta taaatggtta
      601 ttgacctttt ttcctttcaa atcgttgata tggcattcgt ttacggtcaa gttcattttg
      661 gatttcttct gtatcgtcgt aagtgtaggt gtctaaaaaa tcagttaaag tcgtgtacca
      721 gtcgtcgcct gggttatcta cttcttctac atattccctc aaaaatgctt cgggtacagt
      781 gagttcataa tccattccca ttaacacgat agattccgtc ccacacgtca aaaatctagt
      841 aaatacaagg taataatcgt ctaccgtcgc atctttgctg tttagctctt ccatttcttc
      901 ttccattaat tccatgctct caaaaattga ataatattct gattctgagt aatagtgttt
      961 cctgcaaatc gtgtagctga caaagttttc gaccagctca ctcctttcta ggtaaacatt
     1021 catgatatat ttttctgttt ctagtcgtag aagctccgct ccttttttta atgtgtcagg
     1081 acgacgttta tacacgtcta aacgattatg gctgactgta accttcgttt gactcgtttc
     1141 actttccata tacaactgtt cttccactct ttgaataaag tacttcattt cttccataca
     1201 actaaagtct ttttgaatcc gtctgattac ttcattcaag tgttctttgt ttgttaaaaa
     1261 ttgcgaaaca tcttctgttg ttagctgata aaccacctta ttcattatct ttctcctccg
     1321 attcaatcgt ataaaatgca ccattttttt cgatattttc aaaagtatct agccagtcgc
     1381 tagccattgc tttagctttc ttaatctgtg cgaattttgc ataagtcagt tctaaggctt
     1441 gatattcgga aggtttgata gtgcttggtt tgacacctac ttccaagcat agtaaggctt
     1501 ggaaaaacca ttttccattt tgttttttca gttcttccag tgttcgatag tacgtcgcaa
     1561 tgagattcat ttcgacacct gattcaatca tttcttccac tgcgactgac aatttttcct
     1621 tgtaatgttt tttgggtaca cgtgtttcat ggtataggaa cgtcatatcc tccatttttt
     1681 gtgccatttt atccaaaggt aatttgacaa attcgtcata gtaattttga gccatatttt
     1741 ttctcctttt tagttctcta atttggttaa ccaatattcg atttcataag tttccatgtt
     1801 ttcttgataa tgcagttcat atcctgcaat cttcactagg tcttcaaata gcatagtcgg
     1861 tgcatcctct actaaagggg tttctatcgt ccagtaccgc ggaataagta aagcttctgc
     1921 tactttttca gaaatgactt ctttgtccac caaaatttga acgacttctt ttacagtcaa
     1981 taatttgtgt tgtgttttca tcaccgtttc tttactttct catcaatcaa aacataatcg
     2041 ccataagaca aggtatattt tttcccgcat gttggacatt gatacaaacg atagcttttg
     2101 atacatttcg ctctttctcc acaatcgcat tcgacagtat gtggtgcatg atagtttggg
     2161 aataattcgc tgaagggaac accccaataa tcaaatttct ttcgcatgat tgattctgcc
     2221 tcctcttagg aaaggtttta tttcctttcg ttagttcttt ctttattgat ttgttggcca
     2281 gaatgaatca acgaaaagcg acaaggacaa gggcgcgcag cgtgccattt acccttgtcc
     2341 ttgtcgcttt ttggtgataa aaggcaactt gttgccggtc tggcaaatca ataaagagat
     2401 agcgaacagg aacgaacctt ttggtgagag gaagtttttt ctattttgtc ttgctttgca
     2461 agacaccata tcttagtgaa cgtgcaacgt tcacttcctt tcttctcgaa cggcggtggc
     2521 ggttggagtg agagaaaggt ttttcaattg agaaggcact tgtgtcttcc taaaaaggtc
     2581 ttttcctgtt tctatgtaaa acaaaaagag ctgcaatcct catttaggac tgcagctctt
     2641 cttttatatg cccatcgatc gcgtaaaatg atcggggttt ctaatttgca tcgattcaat
     2701 gactaattga ttccgtttat tgaaatgacc tgtgacggca atattgtatt ttccatcatc
     2761 cataattaac aatttatcga taatttcaat atctgcaact acacaattaa tcgattcatg
     2821 aattgtttgt aaagtaaagc gaaccaataa tggttgtgtt tttattactc ggattttatc
     2881 aagcaaacca acatatttgt tcatcacgat cacctcgcta aaattatagc aaaaacagct
     2941 aaaattgaga atttttattc gattttagat atgctataat gatcaagaca gtaaagtttg
     3001 tgacggtggc tatccgattg aaagtgaggt ggtgcgtatg tgcgtacttc caaaatctta
     3061 cagaaaggag aagccttttg cctgttgaaa cggcgctagg attaatgatt agtttcggtt
     3121 ctttgatcgc aacaatcatt tttggaatct tagcagttgt aaacaacaat caaaaaaaat
     3181 aaccgtcagc tttagccggt cgacgattat ttttcaaaaa taatattaag ctggtcaccg
     3241 tcttaaacgg tgctgttagg ggagttgtta gcgcaactcc tcttttcgta tccattataa
     3301 caactttatt gatcttttac aatcagaatg gtaaatcatc atcactaata tcgattgatt
     3361 gtccgccaaa tggattttgt gaagcgttca atccattgtt gttaggcgta gctacatccc
     3421 gttgttcaaa ggtggtaggg cttggactgc tggacgattg atcagcgtgc gcacggcttt
     3481 ctgtcgctgc tttactttct aatagttgga aattatcggc cacaacttct gtcacataca
     3541 cacgttgtcc ttgttggtta tcgtaggttc gggtttgaat ccgtccaaca acgccgatca
     3601 aactgccctt tttagctaat gtcgccatcg tttctgctgg tttgcgccaa atcacacagt
     3661 taataaaatc agcttctcga ttgccgtttt gattcgtgaa atttcggttg acagctaggg
     3721 taaaagtcgc tactgcagta ccactcgcgg tataacgtaa atctggatct ttggttaagc
     3781 gtcctactaa agttacatta ttgatcatct ttatctctcc atttcttgat tcatttgttg
     3841 tgatctttta aactgccgtt ttctttcttg attttcaatt ttgatcacac ttttcattag
     3901 gtgatcaatt tcgttgactg tatctgtaaa tgaaatggaa ttcgcaaacg tgttaactga
     3961 gcgatatttt tcccactgct tttccaaata gtcatttcct ctgatcttat ccatttctct
     4021 ttcataataa aaggacaacg gttttgtatc cccacgtttt gtaaatgtat ttcgaacact
     4081 tttgtataac tctttcgtat cgatattttc tagtttcgga atggtaaata agtcgtagta
     4141 atcttttgct ctcgaattat ctgtgccaaa gctgaacgta gcacttagct tttctgcaat
     4201 aatttgttct gttggataag ctggaatctc cactttttta tcttcaaaca ttaattggtg
     4261 atagtgtgta tctacttcag gataaataga atctcctgtt gtcacgtcca ttttaaacgg
     4321 tacacgtact ttctctaaat tagccaccaa tttaatttga aatccaggat aaaaatcggc
     4381 ttctcttgtt tccttcattc cttgaataga gaaaacaatg ccttcttttg tgggttttct
     4441 agttaaatca ttaaaaatgc tcttcaattt ttcttcagtt aatttgctat ttctaaatgt
     4501 cgtatcgata tcaattgttg tccttctatc gattccatat tttgatccta ataaaaagcc
     4561 gcctttcaaa atgaagtcat ttttgtactt tgattcagaa atttgttcaa ttagacgatc
     4621 catcataaaa tgccgttgca ctaactgtgc atttaatcct gtactctttg ctaaatttct
     4681 tgtttttgct gcaaattgtg tcggtgtcat ctttttcctc catcaatata agctttgcat
     4741 gtggtatttc atttcttttt ctacatgtag ttttgtcatg taatctctta atttacttgg
     4801 atctctcaat gggtcttcca tgtactcttt taatgcttct aatttcattt catattcaaa
     4861 ttcatatctc ggattccaaa tatcacaaag tgtacgctct ttatcataca tagtaatagg
     4921 atttccttga aaggaagttc cctctacttt tcctagttca taaacttctt tcttcacgaa
     4981 atgaaattgt aactgctgct cttttgtaat tgaacgatca acgttatagc catggggaaa
     5041 agttaagtga atgaatttcg gcatattcgt agaaagctgg taaatatcta aagtcgtttc
     5101 atgagagaag attcccctgg aaaatcgttg ctgaagccaa aataaatcat ctattccatg
     5161 accatcctct acataataac cacgatcaac tttgatcaat tttccttgat tcaccaatct
     5221 ttgtacagtg gttgctgcga ttccaaattt cctggcttcc ttcatagata aatttccatc
     5281 gcgaatagcc aacaattctt tcagtttatc ttgcatttac ttcccctccc ttcgttacaa
     5341 atacactaca tcaaatatat catagtagtt tatttgtaac aagattaacg tctatcgatc
     5401 taattcgatt tctttttgaa tgcgctgctg gttctttaat tgttgcagtc ttcttttatt
     5461 tgcacgtaat ttctcttgtt ctaaacgaat ttttgctttt tgattttcga tttcttgttg
     5521 tttgtgtgtt gtatttcgca taggataacc ccttcattat tttgtagtgc ggcgtgttct
     5581 tttcttatag ttcgtttttt tcttttcaga aaattcaaag cctgttgtaa agtcttcttt
     5641 taaaacgatc gttgcttcaa acgaaccttt ctgacctttt agtcctctga ctgattggct
     5701 tgttcggccg ttttgaatga tctctttcag ttgtgtggct gtgagtttct ttttggcaat
     5761 cgttgtccat aatttaaaat cacaggcttc agacgtacat gccgccacat tttgataaag
     5821 caagacttct tgttcccgac attttggaca aacacctaaa tgacgcgttg cttcctgttt
     5881 ttcactggct gctttcacct cttgtaacgt tgatccaaaa tctgttgact ggatttgtcc
     5941 cggtacgact tccaacaagt gagagacaaa tttttggata ttagctaaga aagtagcttg
     6001 tgttcctttc tgctcaccaa tctttaataa gtaactttcc catttggcgg tcatttcggc
     6061 acttgttagc agcggctctt tttccacggc ttggcaaagt aaaatccctt tatttgtcac
     6121 cacaagctta tttttttcta ccttgatata ttccttttgt ttcaaagttt caataatatt
     6181 tgctcgtgtt gcttctgtac cgattccttc tacttctttc aatatggatt gtgcttcttc
     6241 gctatccacg gtttttccag ctgttttcat cgctgtgatc aacgtacctt cggtataagg
     6301 ttgtggtggc ttcgtttctt tttcaaggac ttgtagatct accgtcaccg cttctccttt
     6361 tgtcacttta ggaagtagcg gttcttcttc cttttctttt ctgtcgctct ctttgccaaa
     6421 cagctttttc caacctagat ctactggtac ttttccgact gtctttagct gaagctgctg
     6481 aacttgcgtc aagatcgttg tttcttcata ggtatacttt tccgcaaaca tggccaccgt
     6541 tgtttttaac acttgttcat aaatagcctg ctgcagtgga cttaattgtt caaaagccgc
     6601 tgccgtcatg accgtctttg taggaatgat cgcgtaatgt tcctggactt tcttattatt
     6661 cacatatcga ctattcggtg tatgaatagg agtaggaatg gcttcggcct ttaaaaagtg
     6721 tttgtattcg tctaagtgat ccaacagata cgcatactcc ccttctgtaa tataaggggt
     6781 atctgttcgt gggtagctta gatatttccc ttcatacaac ccttgcatgg cttccaaagt
     6841 atctttggca ctagctttca tgagctgatt cattttagat tgaaggctgg atagagaaaa
     6901 aagacgtgga cttgccgcac tttttttctc ggtttcaact tggtggatcg tacctggctg
     6961 ttttcccaat tgaaccccct tttcttttaa ataatcttca aatgcttctt gcgtcgcttg
     7021 ggtttcattc ggatcaagct tcgcatcaaa tgcgccattt tgagcgatta cttgggcttt
     7081 tccttcaaaa tatggttctt tttggaagtt tctaatcttt tcctgtaatt gataaatcat
     7141 gtaaagcgtt ggggtttgga ctctgcctaa ggaaaaactt cctggtatgc ctttttgctg
     7201 caagagtaaa ctatacagcg ggctaccgtt catgccgatc aaccaatcgg cgatctggcg
     7261 tgcttgtgct tcttcaaatt ttgggtaggt ctcttctgca tctcgtaact gctggaatcc
     7321 ctgataaatg gcttcttttt ctaaggagtt gatccaaagt cttttaaata tctttccatg
     7381 atcggcgcct gcttgttgaa tgatcgtcca agctatcagt tccccttcac gatcgctatc
     7441 tgtcgcaata ataatggtgt cggccgattg aagttgctgt ttcaccacct tgaattgctt
     7501 tttcttatct ttaggaacat gatagtgata atgtgtaggg aaaataggca aatgctctaa
     7561 tgaccattgt ttccaatttt catcgtacat atcgggcgaa tctaactcga ctaaatgacc
     7621 aaaaccatac gtgatcacag tttctcctga aaacaatcga tcttggatct caaaataccc
     7681 atcttttcgg gtagctttag aaaagctatc ggcataagcc ttcgcttgtg acggtttttc
     7741 tgctaaaata acggttttca tcatgacgat ttcactccct ttcttgctgt ctaccttgtt
     7801 gaaacaacat ggcacgtttt tgctttctga tctcttgttg gtagatttca tgcttcaatt
     7861 gatcattcca atcctttttt acttcaggta caaattcatg ttcatgattt ttatagcctt
     7921 tgtagtattg ttctgcttta taagagaaaa gaatctgagc attaatatcc cctttttctt
     7981 gataaaaacg atcaaaatcg tacgtaccat ccacgcgttc acaagctttt aattcttttg
     8041 cgacttctct acaaatatct ttcaaattaa tagcttctat tgaatcgtta acagaaagct
     8101 gctgaccaaa agacgcattt actttgtgag cggaaaccaa ttggttcgtt aattgcagat
     8161 tgttttctgt cttgtgtatg gccataatag cttctggtgt gatcttttct tcttttgcga
     8221 cttcttcata aatgactttg tattcttttg gcacttgcca atcattagcg attcctcgtt
     8281 cacaacgtac tttctgattc gtaaatggat caactgcgcc cattaattgt tctttttcgt
     8341 agaaaatatg tcctgcacgg tcattatcca cacaaatctc gatggattga ggaaaggctt
     8401 gtttctttct caattcagat actgcctcac caaaataatg actgatcaca tggtgtttta
     8461 acccttccat cgaaactaac cacgtatcgt ttaactgatc acgatttagt gcagcatagc
     8521 taagtaaatc aatactactc tcaaaaaatt ttaactgctt agggtcaccg atctttaggt
     8581 taaagccatg attcgctgtc gaatttttat ctatatgctt atatgtgcct ctctcaccaa
     8641 aacgttcata atcaatgaac gttccttgat aactcgcccc tactgtctca tttgtctgtg
     8701 gatctttcca taaaaaggcg accgcatgat ctaaacgatc gtctcgaact aagaaatttt
     8761 gccgttcatg ttttggcttt agttcggcaa tcaacccttc tttttctgca taatcaatca
     8821 gccactctgg tagttttcgt tcttccttca gataattcct tcctttttct gtgagtggtt
     8881 gataatcaac ggggtgatct ttgtactttt ctgtctcaaa atgaagattt tccactcgtt
     8941 ctgttttgtc ttcattcttt agaataaaat cagtggctgc tttaaattgt tgcttagggt
     9001 cttggatctc tttgtctatg aaaaaaagtt tagctaattc aataatgtcg ccactaatat
     9061 tttgactgtt ccaataaaaa cgttgttttc ttcgatcaaa aacaaatgaa tcatggtctt
     9121 ctaagcgata gtctcgtcct ttattgacta cagccattcc attgtttcga gcaaaatcca
     9181 ctatatcgat ctgtttacat cgatcaataa gactcatttt tttctcggtc attttaaaac
     9241 ctcccaaaag aaaaaagact atccgttttt cagatagtct tttttacttg ttttatacac
     9301 gttctttttg tgacaattgt tgttcataat agatttcatt ttttcgtttt gccacttctg
     9361 acactgtctc aagtgaaaat cggaatgaat ttcctagcgc aaattcttct gaaggtaacg
     9421 ccgtttggat ctcttttcca taactttttc cttttaaaat tacttgatta tgctgtggat
     9481 cttcaataaa cgccgcatcc ttcaaatgta acgttcgacc cgtatcttct tccattactt
     9541 tcccgtcttt cttgtcgatc caaagtgaac caacaggaac aatattcttt tgttctgccg
     9601 tttctacagt atttttatgc tcctgcttca cttctgctgg ttggatctct ggttctcttt
     9661 ctctgtattg attgatttca ttagaaatgg tttgaatgaa ttcatttgcg acacggctga
     9721 catcattcat gattttccca cgttccgcag cttccttatc atccaacgtc tgaccattct
     9781 tcgtccatgc tgcaatataa ggaatagctt tctccgaagt gtccattcca aaatgtttgc
     9841 acacaatata agaagtcatt tctgcctgca attctttagt tgcggtatct aattctgaaa
     9901 atttgtgcat tcttgcatgg gctaattcat gaatacttgt agctaaatgc tgcgtaggag
     9961 tatttcgagt attcatgacg atttcttctt cgccagtaaa ttcgttacga acatatgccc
    10021 cccgtgcttg tcctaattct ctttgataga cttcactttc tgtcatatct cgaatttgta
    10081 ttccaatatt gttggctacc gcttgaatcc cagcttgaag ttctgactgt ccttttttat
    10141 ccaattgaaa atcaaagaca cggttcggaa agattttcgg atagtcttca ggttttaact
    10201 gtgtttgtgt gatatcaaac actttttcaa tcgtgtagta aggttttttg atcacttcga
    10261 tttgaccact ttcgattcgt tttttatctt ctggtgtagc ttctgtttct cgaacaaagc
    10321 cacgtccttt ctcataaaat cctttgaccg tttttctgac aaagatcttg ttcccttgtt
    10381 cccctttttt gatatagatt ccttcttttt tcaacgcagc acgacctaaa caagccgttg
    10441 cacctggaaa ttgttcctca atcaataatt gatttctaaa ggatcgttct ggaaaacgag
    10501 acataaaatt caaaagttct ttcacgtctt caggagaatg ggtatgcttt ttcaccgcat
    10561 ccagtgcctg ttgcgaaagg gatttgactt cttgcttaat ctcttctgcc gttcgttttt
    10621 gatagccctt ttgttttgtt tttgtttctt tcgactgcat ttgttctttc gaaacaggtt
    10681 catttttgac cgtttgtttt tgttcctttt caggtacttg ttcgcacaat tttgctaact
    10741 ctaaatagat gggttgatta ctgttgacgc catcccctag atccattcgg atctgatcga
    10801 ctacttctcc gttcttcact tcttcaaaat agaatttata ataacctgct tctgcggcgt
    10861 attggttatc caacgttttg agttgatcga tcaattctaa tgttacgatc tccccaccat
    10921 aacttttcaa tcctttttct ggatcatttt cgttgaattc tactcgatag aaatgatcgt
    10981 tttctttctc cttcaaagaa tcagttggcg cagcctcttc aagtaattca cgaacagctg
    11041 ctttttcctc ttccgacgtg gcttggtatt tttttgattc ctcaaatccc ccgttttcat
    11101 tcagttctaa agcaactttt cgatagtctt tcaaaaaata aatggttgga ttttttttcc
    11161 attgcgtcac tcgtccgctg tcaataattg cttgtacttc aggaagaaga ttttcttttc
    11221 ctttttctga tctttctttc agctgttcta atgtttccaa tttccgttgg tattttcgga
    11281 tcgtagcggc tgaaaaagtc gattcacctt tttcaaagcg ttcgatttct tctttgattc
    11341 ttggaatatt ttcgattgtc atatccaggc cgccgtactt gttatagccc atttccttta
    11401 gctcttcttg tctttcaagt ttttctacct gtttttcttg ttgcttgatc tcttctctca
    11461 gactatgcac tttgttttct aaacgttctt gttgcctaaa ccaagaatgc ccatttctct
    11521 tatcgttcat tggctgaccg ttggtctttc tctggtgttc aaacatcttc tcaatttgtt
    11581 cgtttaattc attttgcaat cgtactaatt ttcttttgga ttttcccaag cgactattgt
    11641 ctagtggttc tttttcttct gccatgtc
//
LOCUS       DANKAZ010000079        11612 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000079 DANKAZ010000000
VERSION     DANKAZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="PWY67_002107"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="PWY67_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2058340.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="PWY67_002108"
     CDS             complement(322..891)
                     /locus_tag="PWY67_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL2058341.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="PWY67_002109"
     CDS             1077..1979
                     /locus_tag="PWY67_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2058342.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="PWY67_002110"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="PWY67_002110"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL2058343.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="PWY67_002111"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="PWY67_002111"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL2058344.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="PWY67_002112"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="PWY67_002112"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL2058345.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="PWY67_002113"
     CDS             8547..8732
                     /locus_tag="PWY67_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL2058346.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="PWY67_002114"
     CDS             complement(8794..9990)
                     /locus_tag="PWY67_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2058347.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="PWY67_002115"
     CDS             complement(10160..11596)
                     /locus_tag="PWY67_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2058348.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         3422 a         2483 c         2039 g         3668 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cc
//
LOCUS       DANKAZ010000080        11605 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000080 DANKAZ010000000
VERSION     DANKAZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            6..305
                     /locus_tag="PWY67_002116"
     CDS             6..305
                     /locus_tag="PWY67_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2058349.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PWY67_002117"
     CDS             378..1739
                     /locus_tag="PWY67_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL2058350.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PWY67_002118"
     CDS             1761..2021
                     /locus_tag="PWY67_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058351.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PWY67_002119"
     CDS             2035..2787
                     /locus_tag="PWY67_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL2058352.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PWY67_002120"
     CDS             complement(2881..3486)
                     /locus_tag="PWY67_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2058353.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="PWY67_002121"
     CDS             complement(3539..4513)
                     /locus_tag="PWY67_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL2058354.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="PWY67_002122"
     CDS             complement(4891..5610)
                     /locus_tag="PWY67_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2058355.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWY67_002123"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWY67_002123"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2058356.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="PWY67_002124"
     CDS             7161..8558
                     /locus_tag="PWY67_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2058357.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="PWY67_002125"
     CDS             8639..9079
                     /locus_tag="PWY67_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2058358.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="PWY67_002126"
     CDS             9227..9553
                     /locus_tag="PWY67_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2058359.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="PWY67_002127"
     CDS             9554..10303
                     /locus_tag="PWY67_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058360.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="PWY67_002128"
     CDS             complement(10383..10838)
                     /locus_tag="PWY67_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2058361.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="PWY67_002129"
     CDS             complement(11265..11540)
                     /locus_tag="PWY67_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058362.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3664 a         2013 c         2357 g         3571 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaag
//
LOCUS       DANKAZ010000081        11149 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000081 DANKAZ010000000
VERSION     DANKAZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..151
                     /locus_tag="PWY67_002130"
     CDS             <1..151
                     /locus_tag="PWY67_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL2058363.1"
                     /translation="SLPAVYFTYQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFL
                     YKNQN"
     gene            complement(222..413)
                     /locus_tag="PWY67_002131"
     CDS             complement(222..413)
                     /locus_tag="PWY67_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058364.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(554..874)
                     /locus_tag="PWY67_002132"
     CDS             complement(554..874)
                     /locus_tag="PWY67_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058365.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(897..1331)
                     /locus_tag="PWY67_002133"
     CDS             complement(897..1331)
                     /locus_tag="PWY67_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2058366.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1666..2304
                     /locus_tag="PWY67_002134"
     CDS             1666..2304
                     /locus_tag="PWY67_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2058367.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(2628..2711)
                     /locus_tag="PWY67_002135"
     tRNA            complement(2628..2711)
                     /locus_tag="PWY67_002135"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2675..2677),aa:Leu,seq:cag)
     gene            3239..3739
                     /locus_tag="PWY67_002136"
     CDS             3239..3739
                     /locus_tag="PWY67_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2058368.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(3929..4273)
                     /locus_tag="PWY67_002137"
     CDS             complement(3929..4273)
                     /locus_tag="PWY67_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL2058369.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(4427..5347)
                     /locus_tag="PWY67_002138"
     CDS             complement(4427..5347)
                     /locus_tag="PWY67_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2058370.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            5534..6025
                     /locus_tag="PWY67_002139"
     CDS             5534..6025
                     /locus_tag="PWY67_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL2058371.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(6049..8424)
                     /locus_tag="PWY67_002140"
     CDS             complement(6049..8424)
                     /locus_tag="PWY67_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL2058372.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            8750..9376
                     /locus_tag="PWY67_002141"
     CDS             8750..9376
                     /locus_tag="PWY67_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2058373.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            9399..10103
                     /locus_tag="PWY67_002142"
     CDS             9399..10103
                     /locus_tag="PWY67_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2058374.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            10295..11128
                     /locus_tag="PWY67_002143"
     CDS             10295..11128
                     /locus_tag="PWY67_002143"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL2058375.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         3319 a         2179 c         1926 g         3725 t
ORIGIN      
        1 ctctttacct gctgtatatt ttacttatca aattttttct tttaggcatt gggaaacaac
       61 gtctgtatta ataacacaca tcatcgaatt gacgctttca gtaatttttc ttattatttg
      121 gatctatttt ttatataaga atcagaattg aatccctcgc atgtttaaaa agaaaacgat
      181 taaagatgca gcaaatttct gccgatcttt aatcgttttt tctattctgt tataccaaaa
      241 aaatgtttga atcggtgaag gatcgttcct tgttttaatt ctataaattc ttgtgcgatc
      301 gggtactttt cttccaaatt ttctttgttc ttttcgatca ggcttttcgc ttcttcatat
      361 tttttatctg cacatagtgc ttttacttcc tgtaatactt ttcctgcttc catttgcctc
      421 accctcttgc cattttattg ctttaagttt tatttaagtt atacgcatta ttctagtttc
      481 atcccaaata accttttttc atttttactc ctcgaactaa tcagttttct aaaggctgat
      541 tggttcgtct tttttatttt tcctccattt cattctgctc ttctgttaat ttatctaatg
      601 ctttgattga tgctgcggct cttgcataat catagctgta taattccata aatttatcag
      661 gcagaacaaa ccacgctgga tgataaccgt tagctgtgac agggaatcct ttatcttgtt
      721 ggttatctcc atcccatctc agagcaacac ctttttttcc tttccacttt actaaagcga
      781 tcgagtatcc cttctttgtt tgtgtgccgt cttcaataat tgctaaaatt tctatttttt
      841 cttttggcgc acggacttct tgaggacgaa ccattttcta ttcctccctt atctcattac
      901 ttcacgacca acacactgca aggggcatgt gatacgacat aagaagcggt cgatccaact
      961 aatgcttgtt gtatagcacc ttttcctgtc gctcccataa cgatcaaatc aatattttct
     1021 tgtccaggga tgacattggc aatcaaattt cttggatctc cagtttcaac tattgcgtgg
     1081 atctcagcaa tgccgtactc tttggcatca ttgatttttt tcagcatggc tacttctgtt
     1141 ctttgttttt catctgcata gatttttgaa aaggcatagg cactatgggt cagctcaact
     1201 ttgttgatga ttgacacgat aaataagtgg gcttcattgt cctttgctaa gtcaagggct
     1261 tcagcgaacg ctttgatcga ttgtcttgat ccgtcaactg ctaccatgat gttctggtat
     1321 cttttactca ttggaaccgc ctcctttttc ttttattgta acatctcaag caaaaataca
     1381 aaaaatatac gcaatatgta aataaaatga tgaacaaaag caatttcttt caagttattt
     1441 atacatttct tttctcatca gaacacctgt tctaatccca ttatttctgc attatgtgaa
     1501 attcctcatc atttttcaca tcgagcgaac atcattctgt tacaaaaaac gtggatttgt
     1561 cataaattgt catgcttctt ttacctatga aatattttca cgtgttaaag tattcctcgt
     1621 tgaacacaac aaataaaact ttttaacgaa taaaggagat ttttcatgac atcacttaaa
     1681 actttacttt ttggaacaac tttggctgcc ggcgctgcat tcttcatggg aacgactgct
     1741 catgcggacg aagcttatac cgttcaatca ggtgatactc tatcaacgat ttctcaaaaa
     1801 tacgttggtg ataactcctt gatcaatgcc attgcagaat caaattcaat ttctgatatt
     1861 aacctgatct actcaggaca acaattaaca atcccaacag aaggctctgc acaagcagct
     1921 gctgaacctc aagcagccgt tcaagaagca ccagtccaag ctgaacctgt tcaagctgaa
     1981 caacctgttg tacaagaaac ggttcaaacg gaaacacaag cagcgcctgt tgctgaaaca
     2041 caacctgctc ctgctgtaac agaaactgct gctactccag caagtacaag ctctgcaaaa
     2101 gaatggatcg cacaaaaaga atcaagcggt tcttacacag caacgaacgg acgttacatc
     2161 ggccgttacc aattagattc ttcttactta aatggtgact actctgctgc aaaccaagaa
     2221 agagtagcag aacaatatgt tacttcacgt tatggttctt gggaagcggc gaaagcattc
     2281 tgggaagcaa acggttggta ctaagattca ttatttgaac gtttaaacac attcaaaata
     2341 tagtaagagg tcatgcgatt tattctttcg catgacctct ttttgagttt aagtttagtc
     2401 tttattcctt aactatcgtt tgccagtatt tcttgtatca gaatctataa atctactatt
     2461 tgaatcaatc tattaggttt taaactgtcc caccttcaca caaatttttt ttgatttatt
     2521 tgaatctggt tccgttgttt aaaatttttc ctatattctt ttagataata tgttattttc
     2581 tttctcatcg ctccaggtgt accttctctt tataaaaaaa caaattatgc ggctaagaag
     2641 actcgaactt ccacgaggtc accctcacta gcgcctgaag ctagcgcgtc tgccaattcc
     2701 gccatagccg catcacatct tttagtttag gattacatgt gttttttgtc aagaacatat
     2761 ttttgttctt tttcatttct gtacgccctc ccttatgcca tctacacaaa taattcatcc
     2821 gagcaactcc atcatctatt gagggttggt gaaaaaatct gttttatcca tatgatcgat
     2881 ttcaatgtta aacgaacgac tctgggatta gtaactattt tatttttttc ttcatttctt
     2941 ttttcctctt tttctcgatt cagctatctt ctttcatttg gatattgatt atttcttctg
     3001 ttgattacta tccttctgga attctagacg ctgaagtaat tcacgttttc tttcttctag
     3061 agatcataag taacgaatga acgaacttca aataattttt ttattgtaat tcatatttgt
     3121 attgttcgat gattcttaat acgatatgac acaataaata aaaataggca tggctattta
     3181 caacaatagt gctatagtag aaaacaaatg aataaggtta cttaaagagg tgaatacgat
     3241 gaaactgatc aaactatggg aagcagattt aaagaaagct tatcagctac aaactagctt
     3301 tggaccaaat gagagtggat ttatcaacaa tgcttatggc tatacatttg ctgaattttt
     3361 agattatgta aaaaaatgcg aaaaaacgtc tttaggaatc gatcttcctg agagttacgt
     3421 tcccgcaacc gtctttattt tagaaagtga tgaacatgag tatgtaggaa tttttaattt
     3481 gcgccataaa ctgaacgaaa cattaagaaa cggcgctggc catattggtt acggcatctc
     3541 ccctaattat cgtaaaaaag gatatgccac acaaggatta gcattagtac tacaagaggc
     3601 aaaaaaaatc ggcattgatg aggcactttt gtctgtaaat aaaaaaaatc ttggcagtct
     3661 taaagcacaa caaaaaaatg gggctgcgat ttatcgtgaa gatgagaaag aatactatac
     3721 gaaaatcaaa ctgatgtaaa aaagaaggat tccaaaagga aagggatttg aatgtccttt
     3781 atcaatagaa cacatttatt taattaactt ataatatagc agacaaaatt ttataaacaa
     3841 agaaaccttt tatctctttt cttctagaaa ggataaaagg cttttttgtt tttcctattt
     3901 gcttctcata gctagtcagc caagatttct atcgtatccc caacgcaatc cgtgcataac
     3961 ggctcatctt atctgtcgtc catgctggat accagactag tttgacttct gctttcgtta
     4021 cttctggcac ttcttttaat gcgtcatgga tagattctgt taaaatatct gctaatggac
     4081 agcccatagt tgttagagtc atcttaatga ctgtttctcc attttcagga ttgaattcta
     4141 tatcataaat caagcctaaa ttgacgatgt cgattcctaa ttcaggatca atcaccattt
     4201 ccaaggcagc aagaatacgt tctttgatta gttctatttc ttgtccagac cgttcttggt
     4261 ttgcttcgct catcgtacga cactccttaa cacgattttt atttatcata ctcttttttt
     4321 agctgcttgt aaatgagaat ttagaagtaa gattctcgtt taaatttata aaaatgttct
     4381 tattagatgt aatagattaa atttattcta aaataaacct gattcctcat tttttttgtg
     4441 aacgattttt tatttctggc aatacgccag ttgttcgata cattttttgt tcgttttcat
     4501 ttgcaaaccg gccaaatact gaatgagttg gtaaaatatt gataaatgca tcgtcgtcga
     4561 tttcatgaac gatctgatca agatcataca tttcgtagcg ggtgatgacc atcatgatca
     4621 tgttcccttg tttttttgaa tagccaccga cagacggcag taatgtcatt ccacgtacca
     4681 tccgttttgc tagttcttct gttacctctt ctggcttgac ggtaacgatc attgctgtta
     4741 ctttttgatg actcgtgtgg atggcatcaa ttacttgagt cattgcataa atggaaataa
     4801 tcgtatacaa ggcactttcc cagttaaaga agaagcctgc gatgactaca atgataccat
     4861 ttagaatcag catgtagttt ccgactgttt ttccagttgt ttgtgaaagt acaagagaga
     4921 tgatatccat cccaccagtt gtaaagccca tttttaaaga taatcctacg ccaagaccta
     4981 ataaaacacc gcctactagg gcattcatca aaatattatc agtgatctgt ccgactggaa
     5041 gcagcatcgt catcaccgat acacatacaa cattcacaaa actaaggatc gttgctcgtt
     5101 ttcctacttt gagaaaccct aatacaaaca cagggatatt caaaagaaaa ataaatgttc
     5161 ctgtgtctat cttgatatga aaccattcat ataaaagcga ggaaatgatt tgagcgatcc
     5221 cattcatccc tgcagaaaag acattggctg gaatcaaaaa ataatttaaa gcgaccgcag
     5281 cggtcaatcc ggttatcaaa ataaccacta ttttctttaa tctttcattt ttatgatacg
     5341 cagtcataat tttactcaca agcacctctc ccctactttg gtattccaaa tgtaagaata
     5401 acagtttatc tattaaaata aaatacttcg aactcaaatg agaagaattt tagcaaacag
     5461 gtaagaatct gccctaatcc tatgataaga tagatgtatg gtaaatattt atatgtatat
     5521 caggagggtt cctatggcaa aagatgctag cttcgacatt gtatcagaag tcaatacaga
     5581 agaagtaaaa aatgcgattc agcaaacaac aaaagaattg tccaatcgct ttgacttcaa
     5641 ggattctaca gtagaaatca aattagagaa cagcaatcta gttgtattga gcgatgatga
     5701 tttcaaaatc gaacagatca aagatgtatt atttggtaag ctgatcaaaa gaaatgtgcc
     5761 aatcaaaaat atccattttt ccgaatcaga acacgctcta ggtggcaaag ctcgacaaac
     5821 tgccgatttg gtcaacggaa tcgatcgtga aaacgcgaag aaaatcacta cagctatcaa
     5881 aaatgaaaaa ctgaaagtaa aagcacaaat ccaagaagat cagattagag tgacaggtaa
     5941 gaaccgtgat gatctacaag ctgtgattgc tttattacgt aaacttgact taccaatcga
     6001 gttgcaattc actaactatc gttaatcaaa aaacgaggct gacgtctgtc attcctcgtt
     6061 tttttgattt actgcttctt ttgataacca aaaacgctgt attccttctt ccaattcccc
     6121 aacacttcgt acttctcttg ctgtttgcca atgttgtggg aataaacgtc gataagaaaa
     6181 actagaaaat cgttgatctg ccaatacaac gattccttga tccgccatgc cacgaatcac
     6241 tcgaccacca gcttgcaaga ctttgttcat tccaggaagc tgataagcgt aagcaaatcc
     6301 aaggtgttcc ttctcatcat aatatgattt gatcagttcc tgctcatgat tcatctgcgg
     6361 caaacctaca cctacgatca tactgccgat caatcggtcc cctctcaaat cgattccttc
     6421 tgaaaaaatc ccgccaagta cgcaaaatcc aatcaatgtt tcagcgggat ttgtttgaaa
     6481 ttttgttaaa aatcgttcgc gttcttcctc attcatgtcc gtttcttgga ttagcacatt
     6541 gctgtcggga tattgctgtc tgaatgcttc caccgcaaga tctaaatatt gataagaagg
     6601 aaagaaaacg aaataattcc cgacctttcc atcagcaaaa gcatgaatcg tctctactaa
     6661 ttttgccaga ctgtgttccc tttttcgata agtcgtctct aggtaatttg cgatgaaaac
     6721 ttgctggttt ccttctggga aaggactcgg cagtttgtaa gccagacttt tttgtccccc
     6781 cagtgtttcc tgataataag agagtggtga aaaactagca gaaaaaagaa tactgctttt
     6841 tcctttatcc aacgactgct caagaaacaa gcttggatca atacaaaact cttttactat
     6901 cagatcgcga tacgtttttt cgactgttgt ttcgtagtga tcatcataaa attcactgac
     6961 cttcaaaaaa tgaagcaaat caaaataata aggaagcaat tgttcttgtg atgggtgctc
     7021 aggaaattct gccagccatt ctttaatttt ttcgcttaat tgataacctg cctttactaa
     7081 agattcagcc ggtgcttttt gatgatgata atcccaatga tcttcttttg ctatttctcg
     7141 gatcgaatca aattctttta atagtttgtt gaaacggcga tgaagttttt tgaattcctt
     7201 tggtatagct tccttacttc gtttcgtttt ttcataagat agttctgccg aatacatctc
     7261 tcgggaacga ttcaccaaat tatgtgcttc gtcaatcaaa aacagatagt cctcgttttt
     7321 ttcatcttca aaaaaacgtc ttaaataaac agttggatca aataaataat tgtaatcccc
     7381 aatgattacg tcacaccaga tactgacatc taatgatagt tcgaaaggac aaagtgtatg
     7441 ctttcttgca taagtttcga tgacttcacg agtgatctga ttctcatgat tcaaaagatc
     7501 ccataaacct tcattaatcc gattgtaata accattggca tatgggcact gatctggatt
     7561 acaggtcgtc tcatctaaaa agcagatctt gtctttcgcc gtcaaagtga cactttttac
     7621 ttcagctcct gtatctttta atgctgtcat agcatcttca gctacttgtc tagttattgt
     7681 tttagccgtt agataaaaga tcttttctcc ctcttcttcc cctacagctt tcaaagcggg
     7741 gaataacgta gagattgttt ttcctgttcc ggtaggtgct tctacaaaca atttctgttt
     7801 cgtacggatc gttttataag cagctaccgc tagttcacgc tgacctttcc tatactcatc
     7861 atgggggaat ttcaaagcca tcaaagaggc attgcgtaca gtgcgccagt tttcttgaaa
     7921 aacaagccat tctttataat cttcagtcag ctttttaaaa aaagtttcta actgctctct
     7981 tgtctgatgt tcaacctttc tcgtgatcac ttcttctgcc gtctggaagt aagtcaattg
     8041 cagatttatt tcttgtaaat catgttcttg actatagata taggcataca cccttgcttg
     8101 atgaaagaaa agttccactt gttctggttc taaatcttca aatcgtggtt cggaggtttt
     8161 gatttcatct atataataaa taccgtctct tttgaaaata ccgtctgcac ggccttcaac
     8221 gatcaggtaa tattcatcta attgaactgt tgtttttaaa aagacttctt tttcatagtt
     8281 ttctcccgca tctttttgga gcttgcggtg gattctcgct ccttctaatg cagtatggtt
     8341 acttttttta cgattgtcga tacttccacg tcgcaaaata aattctacta agtgccgcac
     8401 agatattttt gactgtttga tcatttccac cacctctttc tctctaagat tacctgaaaa
     8461 aacctagttt agcaaaaaga gctctctttc caaacataaa aaaacaccct gctgattagg
     8521 cagggatata ggatatagaa tttggatttt tggatgaatc taatgtaaca aggacatatt
     8581 aaaatgctat gaaaacttac tttccttctc tttagaatat ccaaatactt ttcatatagt
     8641 ctgataattc agataagtaa agagaaataa gtacctgttt ttgtttccta gcaatgaatg
     8701 cgttatacta atggatgaaa gaagttttgg ctatcaagga ggaatgcaca tgcatatcga
     8761 aagattaaaa acaggaatga ttgaagaaaa ctgttatttg gtctacaacg aagaagcact
     8821 tttgatcatt gaccctggag aagatgcgga acgaatcaaa caagaaatca aaaaaacaaa
     8881 ccaacagccg gtagcgattt tgcttaccca tactcactac gatcatatcg gtgcagtgga
     8941 acctttacgc gcgtattatc aaatcccagt ttatgtcagc ccactagagc aaaaatggct
     9001 aggtgatccg attttgaatc tttctggact cggtagacat gatgatattg ctgatgttat
     9061 tgtctcgcct gcagaatatg aatttgaaat gaaaacttat actttaggaa acatgtcttt
     9121 tcgcgtcgtc cctaccccag ggcattctat aggaagcgtc agctttattt ttgatgattt
     9181 tgtcgtttct ggcgacgccc tgtttagagg aagtatcggt agaaccgatc tgtatacagg
     9241 gaatttagaa caattacttc atagtattcg gacccagctg tttgtattac ctgatgaatt
     9301 tgcagtctat ccaggacatg gcgatgcaac gacgattgaa caggaaaaaa ggacaaatcc
     9361 ttttttcaat cgttaaaacg acttaggagg agaataaaat gacagaaact acactttata
     9421 tcgtacgaca cggaaaaacg atgttcaata caattgaacg agtacaaggc tggtgtgata
     9481 cacccctcac aaagcaagga caagaaggca ttcattattt aggtcgcggg ttaaaaaatg
     9541 tcgacttttc atttgcttat tctagtgaca gtggtcgtgc aatcgaaact gcccgtatta
     9601 ttctaggaga acattctaag ggaaaagaaa ttccttattt tattgaaaaa aggattagag
     9661 aatggtgttt cgggtctctt gaaggtggat atgacatgga aatgtggggt gtgatccctc
     9721 gtgtattgaa ttttaaaaca tatgatgaaa tgtttaccac agaagtttct tttgaacaaa
     9781 ttgctaatgc tgtatacaac ttagataccg ctgggtgggc ggaaacttat gaaacattgc
     9841 gagaccgtgt atggacagga tttgaagata tcgcccatca ctgcgaaaaa aatggtggtg
     9901 gaaatgtttt agtcgtatct catggtttga cgatcgcctt tttgcttaat ctgatcgatc
     9961 ctaaacagcc tgttcgtgct gggcttttaa atggcagcgt aacaaaggtc acttatgcaa
    10021 acggtcgatt ttctatccac ggcatcaatg atacgtctta tatcgataac gggaaaacac
    10081 atagtcgcga ctcgtttcta taatggggat attttaattt ttaccagaga atcataaaag
    10141 gcaaagtctg aacgagggct ttgtcttctt ttttctcttc gtgtacttat ctgctttatt
    10201 tctcaagttt ttcttaatat taaaaaacag attgggtttc tcaaaaaata acggtatact
    10261 ataagatgta tcttaattgg gaaggaagga atttatgttt tttgcaaata ttatcaaagc
    10321 agtgattctc ggtattattg aaggaataac ggaatggctg cccatcagta gtactggtca
    10381 tttgatccta gcagatgaat tcatcaaact ggatgccagt tcacaattta tgtcgatgtt
    10441 caatgtcgtt atccaattag gggcgatctt ggctgtcgtc gttctttatt ttcacaagtt
    10501 aaacccattt tctccaagta aatcaacggt agaaaaaaaa gatacgtgga cactttggtt
    10561 taaagtgatc gttgcttgca taccagccat catcattggt ttgcctttgg atgattggtt
    10621 agaagcaaat ttccacaagt tcttaccagt tgccattgtt ttgatcgttt acggtattgc
    10681 ctttattatt gtcgaaaaac gcaacaaaaa caaaacacct cgttggactt ctctgaatga
    10741 ctttacgtac caagcagctc ttcttgttgg gttattccaa gtcttggcac ttgttcccgg
    10801 aacatcccgt tctggcgcaa cgatcctagg atctatcttg attggtgctt cgcgttttgt
    10861 cgctacagag ttttcatttt tcttagggat tcctgtcatg tttggcgcaa gcggcttgaa
    10921 gatcgtcaaa tatttagctg aagggaattc attcaaaatg gaagaaactg ttgttcttct
    10981 tgtaggaaca ttcgtatctt ttgtggtttc cgtcttagct atcaaattct tactaagcta
    11041 tttgaagaaa aacgacttta ccgcttttgg ctggtatcgt attatccttg gtatcatttt
    11101 gattggttat tggtttattg caatgtagaa aaaaccagaa cacttctct
//
LOCUS       DANKAZ010000082        10922 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000082 DANKAZ010000000
VERSION     DANKAZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..281)
                     /locus_tag="PWY67_002144"
     CDS             complement(<1..281)
                     /locus_tag="PWY67_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2058376.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            423..1541
                     /locus_tag="PWY67_002145"
     CDS             423..1541
                     /locus_tag="PWY67_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2058377.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="PWY67_002146"
     CDS             1669..3045
                     /locus_tag="PWY67_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2058378.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="PWY67_002147"
     CDS             3446..5026
                     /locus_tag="PWY67_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL2058379.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="PWY67_002148"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="PWY67_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="PWY67_002149"
     CDS             complement(5652..5972)
                     /locus_tag="PWY67_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2058380.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="PWY67_002150"
     CDS             complement(6120..8354)
                     /locus_tag="PWY67_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2058381.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="PWY67_002151"
     CDS             8833..9099
                     /locus_tag="PWY67_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL2058382.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="PWY67_002152"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="PWY67_002152"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL2058383.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aa
//
LOCUS       DANKAZ010000083        10713 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000083 DANKAZ010000000
VERSION     DANKAZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(231..1079)
                     /locus_tag="PWY67_002153"
     CDS             complement(231..1079)
                     /locus_tag="PWY67_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2058384.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1078..2145
                     /gene="gnd"
                     /locus_tag="PWY67_002154"
     CDS             1078..2145
                     /gene="gnd"
                     /locus_tag="PWY67_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL2058385.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2147..3148
                     /locus_tag="PWY67_002155"
     CDS             2147..3148
                     /locus_tag="PWY67_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL2058386.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3145..3429
                     /locus_tag="PWY67_002156"
     CDS             3145..3429
                     /locus_tag="PWY67_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL2058387.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3462..4259
                     /locus_tag="PWY67_002157"
     CDS             3462..4259
                     /locus_tag="PWY67_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2058388.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4273..5106
                     /locus_tag="PWY67_002158"
     CDS             4273..5106
                     /locus_tag="PWY67_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2058389.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5124..5981
                     /locus_tag="PWY67_002159"
     CDS             5124..5981
                     /locus_tag="PWY67_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2058390.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6003..6512
                     /locus_tag="PWY67_002160"
     CDS             6003..6512
                     /locus_tag="PWY67_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2058391.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6549..6950
                     /locus_tag="PWY67_002161"
     CDS             6549..6950
                     /locus_tag="PWY67_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058392.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7062..7415
                     /locus_tag="PWY67_002162"
     CDS             7062..7415
                     /locus_tag="PWY67_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058393.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7449..7892
                     /locus_tag="PWY67_002163"
     CDS             7449..7892
                     /locus_tag="PWY67_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2058394.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7909..8994
                     /gene="uxuA"
                     /locus_tag="PWY67_002164"
     CDS             7909..8994
                     /gene="uxuA"
                     /locus_tag="PWY67_002164"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2058395.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8987..9700
                     /locus_tag="PWY67_002165"
     CDS             8987..9700
                     /locus_tag="PWY67_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2058396.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9907..10056)
                     /locus_tag="PWY67_002166"
     CDS             complement(9907..10056)
                     /locus_tag="PWY67_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058397.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10452..10652
                     /locus_tag="PWY67_002167"
     CDS             10452..10652
                     /locus_tag="PWY67_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2058398.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3580 a         1694 c         2320 g         3119 t
ORIGIN      
        1 aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg catctaacaa
       61 gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac gttctaaccc
      121 accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta ccaaatattt
      181 ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg ctattctttg
      241 atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt ttcgttgtaa
      301 aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg ggaagctaaa
      361 gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc gccaagttta
      421 gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt ttttcttacg
      481 gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa aacagcacag
      541 tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt gctgtgagaa
      601 gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat ctctgaaccg
      661 ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc tttttttaga
      721 tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga ctcgaacacc
      781 ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat agaaatagca
      841 gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc attataacca
      901 agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact tgctaaattg
      961 ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc gatagcctgt
     1021 tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga gacgctcatg
     1081 agaattcctc attttcataa taagattatc attttttccg ttctcattaa tgttaaagat
     1141 acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat ttacaaaaaa
     1201 atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat tggtgtaatt
     1261 ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca tcacaaagta
     1321 caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga cattcctgct
     1381 tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat ctggttaatg
     1441 cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa actcggacca
     1501 ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg tcgagcagaa
     1561 gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg aatcgaagga
     1621 gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct atccgtggaa
     1681 caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc atccggaagc
     1741 ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca agcaatcggt
     1801 gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca agtcgcacgt
     1861 gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga agatcagtta
     1921 aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg tgaagcaaaa
     1981 tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc aactagtttg
     2041 ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt ttcagcacta
     2101 agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc cagattatca
     2161 tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact tggaaagatt
     2221 aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag gaaaagaact
     2281 agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat tttttcgacg
     2341 agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta ttggttacaa
     2401 caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca tacctgcttt
     2461 agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca tgcttcgacg
     2521 cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg cggagttcgt
     2581 cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata ccggaagcgg
     2641 agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg atccttacaa
     2701 atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag aattactgga
     2761 acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc acatgatttc
     2821 tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg caagaggcgc
     2881 tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg caggatatgc
     2941 cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag cgtttgaaca
     3001 agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag caatgggtga
     3061 aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac gttctgtcca
     3121 gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt aaaagtgaaa
     3181 acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc tgatctcttg
     3241 aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg ggaaaaggcg
     3301 ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca tcgttattct
     3361 ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa aatgaaatcg
     3421 atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat agtgccaatc
     3481 ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct ccatggacac
     3541 ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa cacttttaca
     3601 gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct gttccatatt
     3661 tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc atcttagaac
     3721 aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg aagatctgca
     3781 aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa agaaaactga
     3841 catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta gttggaggat
     3901 taggagataa atacgcaaga aatgcggcag cacatgctat gcatgacgct atcagtaaat
     3961 atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg attttctatc
     4021 aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg tatcgggaat
     4081 tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa cagataatag
     4141 ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct atagaggtaa
     4201 cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga aagatctgaa
     4261 ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc tttatgggta
     4321 gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg tttttctcct
     4381 ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc gatgattgtt
     4441 acagccgctt tgcaaatgat ttatatgggg ggtttttctc caggaggatc aatgccagct
     4501 gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga tctgtcgcca
     4561 gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt gtatcagttt
     4621 cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga agatttgaat
     4681 acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt tttattattc
     4741 gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga tgtaatcaaa
     4801 gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg gttagcggca
     4861 atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt ctttttcttc
     4921 cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt tacttatgcc
     4981 atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa acaagtacag
     5041 cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta tgatgacgga
     5101 ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca ggtgctcctg
     5161 aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt gcaaacgaaa
     5221 ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg atgccaatct
     5281 tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg cagttactgt
     5341 tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg tcttctatgg
     5401 aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag gatgacttga
     5461 tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat gcaatcgatt
     5521 caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa cagggaagtc
     5581 ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta ctaggggtat
     5641 ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaatg caaaacactg
     5701 gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg ggggtgcttg
     5761 cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga cgtgagtttg
     5821 ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct gtcgtaatgg
     5881 gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta ttatggctga
     5941 ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg aacgtgaata
     6001 agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg caagtaatgg
     6061 taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa gttgtagcaa
     6121 aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga caaaaaacaa
     6181 ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt aaagaataca
     6241 attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa cacggaatcc
     6301 ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca gatcttttag
     6361 tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa atgaatgaac
     6421 aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa acaggattaa
     6481 aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa gataaagagg
     6541 gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat cgctatattt
     6601 accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta gtatcagata
     6661 tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc taccgatcaa
     6721 aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact tattacgata
     6781 cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa aggaaaaaaa
     6841 caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg tggggaatga
     6901 tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa acaggtatca
     6961 ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc ggatggccta
     7021 gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa atgatccgta
     7081 cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat ggagaaaagg
     7141 gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat ctagactctg
     7201 ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa gaacagtggc
     7261 tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt gcaagaaaaa
     7321 agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca tttaattggc
     7381 aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa caaggagtga
     7441 gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa ggactgaaag
     7501 atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc ttagagtctg
     7561 gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg atcaacacag
     7621 cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat caagcgctct
     7681 tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata gggggcgtga
     7741 atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg atagaagaag
     7801 cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat gcttcagcgg
     7861 aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat gaagtgggga
     7921 tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg ccaaataccg
     7981 ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt ttgggagatt
     8041 tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact gcttggaatt
     8101 gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga ttactatatt
     8161 gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt gatttgttat
     8221 agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa caacgatgga
     8281 agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg agaaatgtat
     8341 caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga agaacggttg
     8401 aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga attatttgaa
     8461 aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga tgtaaaaatg
     8521 gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt gacgaaaaat
     8581 cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg actaacgctg
     8641 tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat ccatgaggta
     8701 ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga aagaaagttc
     8761 aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat tatgaaagcg
     8821 ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac cgtgtggggt
     8881 gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac ttacatgcaa
     8941 ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga ataaaatcaa
     9001 gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag cttatcgtgc
     9061 ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct attatttact
     9121 aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg gagaagatgt
     9181 tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg tgaaagaaga
     9241 taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa aagaaagagg
     9301 aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt atttaaaaat
     9361 ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa aaaaaggaaa
     9421 gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc caagtgaagc
     9481 acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg gcattttgtc
     9541 ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc ataagttgaa
     9601 agtagatgta agtttatatg aatccaataa agcagactat gaattttatg acctgcatca
     9661 agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac tattttctag
     9721 tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag taatttttta
     9781 ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa acgcatctac
     9841 tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata tgagggtttc
     9901 cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa cacctgtcgc
     9961 tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg tatccaaggt
    10021 tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt ctttaaatac
    10081 ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat accaccaatt
    10141 gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa gcaaataaac
    10201 aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt cgaagacaat
    10261 agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa aaagcatgat
    10321 atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac atagataaaa
    10381 ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata cgtatcagga
    10441 ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa ggttttggct
    10501 ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc caaggtgacg
    10561 gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat ggtcaacgtg
    10621 gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac cgtcttttcg
    10681 aaagaaaaga cggttttttt gtacgaaaaa att
//
LOCUS       DANKAZ010000084        10598 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000084 DANKAZ010000000
VERSION     DANKAZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            38..511
                     /locus_tag="PWY67_002168"
     CDS             38..511
                     /locus_tag="PWY67_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL2058399.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            520..747
                     /locus_tag="PWY67_002169"
     CDS             520..747
                     /locus_tag="PWY67_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2058400.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1383..1754
                     /locus_tag="PWY67_002170"
     CDS             1383..1754
                     /locus_tag="PWY67_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058401.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2010..2252)
                     /locus_tag="PWY67_002171"
     CDS             complement(2010..2252)
                     /locus_tag="PWY67_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2058402.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2284..3186)
                     /locus_tag="PWY67_002172"
     CDS             complement(2284..3186)
                     /locus_tag="PWY67_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2058403.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3199..3387)
                     /locus_tag="PWY67_002173"
     CDS             complement(3199..3387)
                     /locus_tag="PWY67_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2058404.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3401..3964)
                     /gene="amaP"
                     /locus_tag="PWY67_002174"
     CDS             complement(3401..3964)
                     /gene="amaP"
                     /locus_tag="PWY67_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2058405.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4002..4895)
                     /locus_tag="PWY67_002175"
     CDS             complement(4002..4895)
                     /locus_tag="PWY67_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2058406.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4973..5896)
                     /locus_tag="PWY67_002176"
     CDS             complement(4973..5896)
                     /locus_tag="PWY67_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2058407.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5945..6295)
                     /locus_tag="PWY67_002177"
     CDS             complement(5945..6295)
                     /locus_tag="PWY67_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2058408.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6328..7230)
                     /locus_tag="PWY67_002178"
     CDS             complement(6328..7230)
                     /locus_tag="PWY67_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2058409.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7223..8080)
                     /locus_tag="PWY67_002179"
     CDS             complement(7223..8080)
                     /locus_tag="PWY67_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2058410.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8414..9223
                     /locus_tag="PWY67_002180"
     CDS             8414..9223
                     /locus_tag="PWY67_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2058411.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9263..9760
                     /locus_tag="PWY67_002181"
     CDS             9263..9760
                     /locus_tag="PWY67_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2058412.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10145..10218)
                     /locus_tag="PWY67_002182"
     tRNA            complement(10145..10218)
                     /locus_tag="PWY67_002182"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10182..10184),aa:Ile,seq:cat)
     gene            10405..>10598
                     /locus_tag="PWY67_002183"
     CDS             10405..>10598
                     /locus_tag="PWY67_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058413.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQT"
BASE COUNT         3064 a         1992 c         1810 g         3732 t
ORIGIN      
        1 ctatatttta gctatcaaag ataaaagagg gataaaaatg aaatttaaaa cattgctatt
       61 aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac ttttttatac
      121 tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat tatttgtttc
      181 tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat tgaaactatt
      241 gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac taaaaaaaat
      301 cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta aatttatgaa
      361 agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg tcgtattcat
      421 tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt
      481 acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat cgttaattta
      541 gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt
      601 acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg tttttccact
      661 ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac ctataaaaat
      721 gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa taaaaaataa
      781 tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt cagatttttt
      841 atttcgattg aataccgttt tatctataaa taagatatgt actgactcat acaaaggggg
      901 ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg ttacgttgta
      961 gttggatctg cttttagtat aatactttat tcattccacc agactgatag cagtttgagc
     1021 agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa gtgcttaaaa
     1081 cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta agctcccata
     1141 tatactgtaa agccttctat atattatgtc atgttgcttt ttaacattta ctatgtagtt
     1201 ttgataaaat gatttgaata acagtgatgg acaattcttt tttattttta acattatttt
     1261 gataataatg attatttatt taaaaataca ttagataaca tttgttacga cacgaaaaag
     1321 cttcacaaac aaatatcgtt attttaaaat gagggcgaac tcacacaatt aggaggaata
     1381 gtatgccatt tattgtttac ttttttcttt cattactcgt gatctatatt ccattaccaa
     1441 catttttcct gctcgtcaat cgtctagcag agcaacatga tacgattgct gctcttacga
     1501 aactttgttt acttctattt gtttgtctag attactttac gagctattat tggctaaaac
     1561 agacaagagt aaaaaagaga tattatttct tccttgtctt tttcaatctc tttgagtttc
     1621 tgatccattg ctttttactt catcagtatc atacgatcca tttgtggatc atctgggttt
     1681 tcaaagtggt cgtattattt tgtatgttca cctttcctat ctcaagaaaa tttagggagg
     1741 cattgtttta ttaaagaagc ttctctgtat caataaagac acatttaagg aaaaactttt
     1801 aaaaatctaa ttgggaattt gcccgcataa ataagaggct gtgacaaaag tcttgtcaca
     1861 gcctcttatt ccgtataaac agtgttcaaa agcttgcatc ttcgataata tgcccaacca
     1921 aatgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat gaagaacagc
     1981 tttttcacga cctcttttta aaagtctttt tacagttttc gagcaaagaa tgatacgatt
     2041 agtactaaaa tgatggcacc aatcaatgat ggcacgattg ccattcctgc aatacttggt
     2101 ccccaagaac ctaataatgc ttgaccaagt gctgatccaa ctaaacctgc tacgacattt
     2161 gcaataatcc ccatcgaaga acctttattc gtgattgctc ctgcaattgc tccgataatt
     2221 cctccgacga taagtgacca aataaatccc atgtttcgtt cctcctaatg ttttacttcc
     2281 ttattactga acccgtggtt ctgtattttc ttttactttt tgtgcgccgc ttccagctgc
     2341 tgattttact ttatctgttt gattcgaagc aaattcaccg gtttttttcg ctgcgtcagt
     2401 tactttgtct tgcaccgttt cttgatcttt ttcaaattct tcttttgttt tgatgtccac
     2461 tacgttagcg ttgacttcga tcacttctaa atgtgtcata ttttttactt ctttgccgat
     2521 tacttcttgc atttcttcaa agattttttc gatatctttg ccatattcca caatgacatc
     2581 catatcaact gctacttgtt ttttaccgac ttctgtttcg atcccagccg ttacattatc
     2641 tgtattcaca agttttcctg ccacattaga gaagaaacca ccgtttgcag ataataaacc
     2701 gtctacttgt tcaattgcga ttccgatgat tttttgcaca actttatctt cataattcag
     2761 ctcaccgcgc attgaatctt cctgttcaga atcatgcttt ccttggttat ttgtatgatc
     2821 agaaccaagt tttccttgtc cgtttgtgtg gtcagaacta agtttttctt gtgctttcgc
     2881 atggtcagaa ggatgttttt gctcttccac aggtttagaa ggatcgattg catcatgttg
     2941 ttctggaact ttagattctg gagttcctgg atgatgaggt gcatgctttt ctgcatcatg
     3001 tttcttctct tctactcctt gttgaacgtt agggactact ggatctggat gtgcagatac
     3061 tggtgctgtt ttgttctttt catcgttcac accatttgat tggtttggtg tcgttgggtc
     3121 tggatgtgca gacactggtt tttggttgtt cattttattg ttattatttt tgttattgtt
     3181 attcatggat gtttcctcct aagatttaaa atagtgatct aagattttcg tttgtttcag
     3241 ataaaaaccg atagcgacgc cgatcgctgc catgatcaag atcaataagg tcttgaaaaa
     3301 gcccacggtg atccataaaa cggctaatat caatccaagt accccaaaga tgatagggag
     3361 cttgtatgtc gaaaatagtt ctttcatttt tggtcccccc ctattctact cgtttgcggt
     3421 cattgcttcg tttagctggt tcttcaaagc ctgtgtattt caccgatact ttcactggct
     3481 cttttgttcc caatatgtgt tgtacttcat tttcgatttc tttcatcaat gttcctgttt
     3541 taccaatgag tgctgaagtt cgtttcaact ccccttttac attgacggcg atcttgtttt
     3601 tcgttgcttt gacatttact tttggtgaat ggatgaattc atcttcatgc aagtgagaac
     3661 gaaccattcc ttcaattgct tttttatcca acgtcaactc cccatcacca tgtttcagta
     3721 gaaagtttcc gattgttttt ggataaaaca ggaaaacaag taacgtgata attgtcaaaa
     3781 tagcaaatgc aacggcaagc caaaatacat attgttgcac gtatgaatta gtgatggaaa
     3841 agaatcgaaa cttctctagc tgaaaaggca atggataggt tgcacttaat agtatcgcaa
     3901 acaaaagcac agatagcaaa agtaatgttc cgatcactcc gatcgttttt acacctttat
     3961 tcattttcgc acctcctcat aggggtgaaa cgatcaactt cttataagtt gatcgcttct
     4021 ccaccagtta ctccgtaaat ttgggctgta acataacttg ctttattcga tgctaagaaa
     4081 acataaactg gtgctaattc tcctggctga cctgcacgtt ctagtaaact ttgctgacca
     4141 aattcaggga tcgaatcaga aggttgtcct tcgtctagtt gtaatggtgt ccagattgga
     4201 ccaggtgcta caccatttac acggattcct ttaggtgcaa gctgttttgc taaaccgatc
     4261 gtaaagttag cgattgaagc ttttgttgct gcgtaatcta ataaatgttc gcttggattg
     4321 aatgcttgaa cagaagttgt tgttacgatt gctcctcctg ctgggatatg cggaacagct
     4381 tctttcacta aagcaaacat agcgatgatg tttatcataa atgtgtcttt gacttgctca
     4441 agtggtaact cagcaataga ctgacaagaa atttgctgtc ctgcgtttag taccatcgta
     4501 tctagcccgc caagttcact cacggcttta tctatgattt ctttaggtgc ttgttcgtca
     4561 cgaagatcaa aaggtagtaa gacaatctta cgtcctgcat ctttcacgta ttttgctact
     4621 tcctctgcat ctttttcttc tcctgggaag aattgaagag cgatatctgc cccttctcta
     4681 gcaaatgcaa tcgctgctgc acgaccgatc cctgaatctc caccagtgat tagtacacgg
     4741 cggttttcta gttgattatt gccttcatag gattcttctc cacaatcagg aacaggaatc
     4801 atattctgct gtaaagctgg tgtatcctga tcttgtttag gaaatttctc cgtatggtac
     4861 aagtttcgcg ggtcgattaa ttttggttct gtcattgctg tattcctcct agtttttaaa
     4921 ttgtattgcc aaaacaaatt tctttgtttg atacctgttt taaatagatt ctttaatttg
     4981 tataatcttt ccggtataaa tggtaaccta aaactactgc tgctatcaaa gagccaaaga
     5041 ctacgagtat aaagcctaga gcagaacttt tacccggtaa tcctcctgta ttcattcccc
     5101 aaatccctgc gaacaacgcc ggaacggaaa tgattaatgc agctgaatct agatatttca
     5161 tgatatgatt tagattattg tccatcatcc cgttgaatag atcaccaata ctctctaata
     5221 aatctcgata gatctcgatc attttttctg tttgtcgctg tctcaaatgg acatcataga
     5281 gtagctgttc atcgtctaaa cgtttgatga aagtttcaga attccataat tgatccattg
     5341 tttcccgttg atctcttaag gtatgatcga tatatacgat gtctctttca agatctgcct
     5401 gtttatacag ttcttcattt tccgttgttt tacgagctgc tttatccaaa gcgtcaattc
     5461 gctttttcat ctctcttagt tctttcacgt aatgggtgta aatatctaat aatgtatacg
     5521 tgatgatctt ttcaaacgag tttaaatgat ttccatgctt tgtcagcagc cgatccacaa
     5581 attcggtttt tctatcgata caagtaatca agagattatc ggaaagtaca aaagatattg
     5641 gcgttaattt tttttcgata cgattatctg actgatctag attaatcact accaaagaaa
     5701 atggatgctc tagtttcgtc tcataaaggc gctccatccg agaaacttct tccggatatt
     5761 cggttcctac gaaaatatca tcaggaagct cgaattcttc gattgcttgc tcaatctctt
     5821 cgggctttgc actatcaagt acgagccaat tatatttttt ttcatctgtt tctattacat
     5881 tcttttcttc atccacaaag tagtatctca atgtatacct cctttttgga ctttctcgaa
     5941 actattagct gaagaagcag ttctttaatt tgatattata atcgagtact gcgatttcat
     6001 tttctaatga aaagctgtca agctgtttgt tgaagtccat gttcaatcgt ttaccgaaat
     6061 aattttgctc taatttagat gtatagatgt ccaataatgg atgctcgatt ttagtcgcat
     6121 attctccaaa ttccgaaaca aaaggaatga tttgaaaaaa gctgcctttt gcaaaagcac
     6181 ctttgtccct tagaaatgga tcggaatcat tgataagcgc gagtgcgatt tttgtatttt
     6241 ccccaacctt ttcaattaac gtctctactg catttttaga cattgtaatt tccattccta
     6301 ctccccctct tcagttgttg tcttctatta tttaacaact acatctgttt ttggttgaac
     6361 ttttgcatat tttggatatt tagggaattt agctacacaa cggaaagccg tcatccctgc
     6421 atatttttcg taatatttct tcgttggccc ttcaaaatcc ttgctccacg gtttcacatg
     6481 tcctgaaaaa tggataatgt aaggattgtt gcgagtttct tcgtattcac gttcacctgc
     6541 tgctgttgga tgtttttttg cttttgccat gatatagcct tgtgcattcc aacgcggatg
     6601 aagtggtaac caacggtcat gtaagatagc atttaatgca tcttggtcat ggaatcgtaa
     6661 tttttctgga ttatgttcga tgaaatctaa tactttttgc gtaatatttt catctaacca
     6721 ttttttcaca ttgatcagca tcaaacctga gttgaaataa cgcattgatt tagctgaaat
     6781 tttcattttt tccagtcgtt gatggaaccc agcgtcttca acagctgcta cgattgaatc
     6841 accgaagtca agacgccata atttagagat atcttgtaaa gcgatcatgt cgcaatccat
     6901 atataagata cgttcaactt ctgtgccgcg gaacaattcc ggaatcgcaa tacgataata
     6961 agctgtttct ggaatacgat cgctgattac cacatttgta aagaattctt tattgatttt
     7021 caagaattcc acatctgagt tcattcgtgc gtttttcact gagaagcgta aaagctgttt
     7081 actttcaaaa tcgatatcat cgtcaatcac atagaaccga acaaatttag acgggttgca
     7141 attatctaat actgagacaa ataatgcagc taaatgtggt acgaattttg tgttacaact
     7201 tgcgactact gctatttctt ttttattcat tgatctcgcc tactttcgtt agagtactgt
     7261 gtgctaattt ttttaagtat aaattggtat acggatgatc tttcaacgta ttccaaggtt
     7321 tatcatgtcc tgtaaagtga acgatcgaag gtttttcatt tccttcttta tactgacgtt
     7381 catatttttc atttggtgct ggatgacgtt caaaaatcag cgaagtctgc atgttccatt
     7441 ttggatgaag ttgttcccaa tcttcataaa gtacagcatt caaagcatct tggtcgtgat
     7501 agatgattcg atcgccattt tcactgaggt aatggatagt tttttctgta atttcttttt
     7561 tattccattg atcaatgtca atcaccatca ctcctgaatt gaagtacaag tcatcggaat
     7621 cgattcctaa tctttccaaa gctttcactt ggcctggatc gataatagcg ccgattgttt
     7681 ttccatttaa cggctcatca tatagtttta cgatatcgtc tagtactaac acatctgaat
     7741 ctaagtaaag gactttctta taatcctctt tagctaataa gtttggtaaa gaaattctta
     7801 agtaagcggt agttgtgata tggtcactga ctaggaagtc ttcgtagata tctttttcca
     7861 ctgtcaggaa ttggatactt gcattacttg aatatttatt taccgtttct tccaatcctt
     7921 ctttactata ttctgataga ccatcgtcaa tcacataaaa ttttatgcgt cttgttttat
     7981 tgcaattttc taatgcagtt gcgatcatca cgctcaagta aggagcataa ttttcgtctg
     8041 aagcagttac cacaggaact acgccgtatc tcttctccat caaaatcact cctttttcta
     8101 atctttttct aacctgttct acatctagaa tattcctcat ttggttttaa agcaaaatat
     8161 atgctttgtt ttcattgaat attttaaaat ttttttatca caaaacgcat cggtaagcgc
     8221 taactattcg tttttcatgg ctttttattc aaaggtattg accaaaaact actatatata
     8281 gccacctttt tcttaagaat gttttttgaa aaaattcata atgacaaagc aagttagttt
     8341 cattttttta aattctcatt taatcttaag gtaacaggaa aatagatatg taaaaagaaa
     8401 gtaggaagat aaaatgaaac tagcagcgat tgatttagac ggtaccttgc ttgatcgaaa
     8461 tggaaagata ccagaagaaa atatccaagc acttcggaaa tttgatgaaa atggcggaat
     8521 cgtaaccgtc gctaccggaa ggaacagcat ttctgctaaa gatatttttt ccgaattagg
     8581 aatcggtggc tatttgattt cttccaatgg ttcactgatt gctgaaatga aggatggaaa
     8641 aatggcgaaa gttctcagac gttcaaaaat agatagcgat acactgagaa aagccttctt
     8701 ctatgctcaa gaagcgaaaa tatccatcat tgctagccga gaaacccaag atgatcagct
     8761 gacattcgat gaatcaaatc tagtcaaaga tgacccttat tatgaacatt tcaatttaca
     8821 gaaccattct tttgatcaaa ttttaaatat gttggaagat ccttctctta gctatcttaa
     8881 attagcatta acagataaga gtgaagaaaa actacagaag ctacaaagag atctgcaagc
     8941 agatggtatc caatcagtat tctctgatcc gcatttctta gaaatcacgc ctacaggtat
     9001 cactaaagct cattcacttg tttttctggc ggattacctt ggaatcgaat cgagtgacat
     9061 catggctttt ggcgatcaag aaaacgacat tgctatgttg aattacagcg ggttgagtat
     9121 tgcgatgggg aatgcacagc gacaagtaaa agaaattgca aaccaagtaa cactgacgaa
     9181 tgaagaagct ggtgttgctt atattttaaa aaacttctat taaaaagaat aaaatgacga
     9241 tatgaatgta ggaggaaaga aaatgttagt tattattgat atgcaaaatc atattcttga
     9301 tccaaacagc gaattttata ttccagagtc agatcaattg gcagaacgta ttgctcaaag
     9361 attagctaaa gcacgccaaa ataatgaata tattctcttc acacgggata taccagttga
     9421 actgaaggac aaagaagaag aacgagaaga tttgcaattg atccctcttc tttctccaca
     9481 agaaaatgaa cgagtgatta aaaagtatta ctttacgatt ccacctgaaa ctttagctga
     9541 aatccaggaa actttatttg aaagcaaaga agaacaaaaa gagatcgaag tagtcgggat
     9601 tgaaacaaac ttgtgtgtac tctctaatct gataggcctg cagagtgctt ttcctgaagc
     9661 agactttttt gttgatccaa tgttagtctc cagtagaaaa cacggagaat ccgctttaga
     9721 gctgttgaaa gatttcaacg tctctatcgt cgaatcataa aaatatctat cttcatttac
     9781 gatatttaac acacaataaa aaaagcacgc agaactgttt tagtcctacg tgctttttct
     9841 atcaaagtag attggctttt ttatgacaca gagtgaaacg gcaggctcac gacctctttc
     9901 ttaaatggtg ttcaatcagc tgaccctcgg tattagctta acaaacggca aaatatgagg
     9961 aactattttt ccgtttgtta agctaatact caggtcaaaa cgctgatttc acaacctctt
    10021 ccttaaacgg tattcaaaag cttgcatctg cagtaataag aacagccaag taaaaacata
    10081 agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
    10141 cttatgtacc ttgtaggact cgaacctacg accggacggt tatgagccgt ctgctctaac
    10201 caactgagct aaaggtacat cataaataaa atttattttt gttacttttc aactatagca
    10261 cgaatgacaa actctcacaa gtttcaaaat ataagtaaaa taatattgtt attcttattt
    10321 cttttacttg tgtcataact attttgttac tttaattttt atttgcttat tttagaaaaa
    10381 atgttattct tttatttatt ctcaatgtta ggagtgatta ttttgccatt tattgcgatt
    10441 ttattcgatt tagttgcggc agcagcttat tttctccaat taaattatca aacctcagga
    10501 attctgttta ttggaatgat ttttcaaggt gtgattaccc ttttgcttct tgtcatgatg
    10561 ctcagctata aaggaaaaag atatgcccgt attcaaac
//
LOCUS       DANKAZ010000085        10313 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000085 DANKAZ010000000
VERSION     DANKAZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..626)
                     /locus_tag="PWY67_002184"
     CDS             complement(<1..626)
                     /locus_tag="PWY67_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2058414.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPR"
     gene            827..2041
                     /locus_tag="PWY67_002185"
                     /pseudo
     CDS             827..2041
                     /locus_tag="PWY67_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2220..2549)
                     /locus_tag="PWY67_002186"
     CDS             complement(2220..2549)
                     /locus_tag="PWY67_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2058415.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(2539..2811)
                     /locus_tag="PWY67_002187"
     CDS             complement(2539..2811)
                     /locus_tag="PWY67_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2058416.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(3262..4065)
                     /locus_tag="PWY67_002188"
     CDS             complement(3262..4065)
                     /locus_tag="PWY67_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2058417.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(4043..5167)
                     /locus_tag="PWY67_002189"
     CDS             complement(4043..5167)
                     /locus_tag="PWY67_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2058418.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(5183..5998)
                     /locus_tag="PWY67_002190"
     CDS             complement(5183..5998)
                     /locus_tag="PWY67_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2058419.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5988..6860)
                     /locus_tag="PWY67_002191"
     CDS             complement(5988..6860)
                     /locus_tag="PWY67_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2058420.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6945..8234)
                     /locus_tag="PWY67_002192"
     CDS             complement(6945..8234)
                     /locus_tag="PWY67_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2058421.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8462..9106)
                     /locus_tag="PWY67_002193"
                     /pseudo
     CDS             complement(8462..9106)
                     /locus_tag="PWY67_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            complement(9583..>10313)
                     /locus_tag="PWY67_002194"
     CDS             complement(9583..>10313)
                     /locus_tag="PWY67_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HDL2058422.1"
                     /translation="DSQVAFQLVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRP
                     CLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVN
                     CPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKE
                     KHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQ
                     ARVTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         3166 a         2178 c         1684 g         3285 t
ORIGIN      
        1 tttctaggaa agtgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtgactgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaattgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaatatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaaggaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaagcaaaat caattctacc
      781 ttagatgttc ttatatcact ctatcagtcc tatttgacaa agagccaaga aaaagacgtt
      841 ggtcgtcgat gctgttctct cgcctactcc tcgtgcttgt agaagttgtg gttctactgt
      901 ggtagatgga aacgggaaag caattatagt gaaaaatggg aagaaggaaa cgattgtccg
      961 ttttgaacaa tacaatcata tgcctttggt tacgcgccta aaaaagcaac gctatacctg
     1021 taaaaactgt cgtacccatt ggacggctca aagttatttt gtccgactca gccattcaat
     1081 cgcagatcat gttcgatata aaattgcttc tttactgact gaaaaagtat ctttgtcttt
     1141 tattgcgaaa agttgtcagg tatctttgac taccgttact cgtacattga aagagtttaa
     1201 aagctattta ccaaagcctt ctaagaagat tctccccaga gtattgatgg ttgatgaatt
     1261 tcgttcgcac gcttccatag aagataaaat gagctttatt tgcgcagatg gtgaaacagg
     1321 aaacttaatt gctgtcttgc ctacgcgtaa attacctcga ttaacaagct atttcttagg
     1381 ctgcaccaat ccagaagaag tagaattctt agtcacagac atgaacgccg cttacttcca
     1441 gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt gatcggtttc atattgtcaa
     1501 acacatgaat caggcactca atgagttgcg tatccgtgaa atgaatgagc ttcgtaaagc
     1561 aggacagaaa agtcaggcag aaaaactgaa aaagaactgg cgctttctgc taaaaaatcg
     1621 tgcaagcatc aaccattatg agtacaaaac atggaaaagt ttccgagcac caaaataccc
     1681 atttctcact gaagcaatga tgattgatcg attgcttgag ttttctgcgc ctctaaagga
     1741 ggcgtatccc ttttttcatg aattagtcga agcctttcga gacaaagacc ctgacttatt
     1801 tttctcctta ttggcagaac ttcccgaaac gttagatgac ggctttcggg agaagcttca
     1861 aaaccttctg acctatgaag aaggtatcac cagcgcaatg atctatcctt attccaatgg
     1921 aaaaattgaa gcgaagaata cccacataaa gacaatgaaa cgagtatcct acggatttaa
     1981 atcattcgag aacatgagaa ttagaatctt tttgatcaat caattaatca atgtaagata
     2041 acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg atttgatttt gcacaactca
     2101 tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa tgaggttcat tctgacccag
     2161 atttgatttt gtacaaccca tcagtcctta ttgacaaaga gcccttcttt ttagtattat
     2221 tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc ttggacttaa ttccccaatt
     2281 tttttccatt tgaccttttt gttgtttttg ggtaattcgt ttagagttcc tacgtcaatc
     2341 caagattctt tttttagtcc tgcctgtttc caatccctta tttcaacgta tttcagcttt
     2401 attttatcgg atttagtatg atattttgaa gtaattgaca gaaacgaaat tgtgtccagt
     2461 ccatttttta gaatatagac aggccggcgt ttaccgccct ctgcagctgt gaaagcaacg
     2521 tagacggttg ctatatcatt agtattcatc ttcattgaac cattcttcaa ttttttcgtc
     2581 agtgtctagt ttttggacag gcaatttttc cgctaatctt tgaatattat ggattcgttt
     2641 acttttttcc gacaattcaa tagaaaatgg gatttttcct tgctcaacaa tttgcttata
     2701 aaaaacggtg acggctgttt tggggctcat gcctagttca tcaataatgc tttctgcttc
     2761 atttttcaat ttttcgtcca tagaaatttg taacttagcc attttctcca cctctttctg
     2821 acaaacacat tatatcacgg aaagagttca aatggaatta atacaactac ttttgaactc
     2881 cttgaaatgc agtgtattta tctaaatata aggaaaggat atagtgctaa atggccttga
     2941 tagatagaaa agtttcaaaa tccgccacag cgataattga agcgaacact caacgacaca
     3001 cagaacagga aaagactcct aaaagaacca aattaactag gacgtgtctg aaaactccac
     3061 aaagatgtgg tatgctctgg taaaaaacga atggcacaac cacgaagacc gatatggttc
     3121 ttggaaaact ttctccaccc tttttgtaat tggagggata gtggtttatt gatagcgatt
     3181 ttccaagttc ttcaagtgga accagacatt gagcaccacg gctaaaaagt gaatgaagtc
     3241 tttattttta atcaataatt tttaatgatt accagtaatt ggttttttca tgcgccctat
     3301 ttctatttga tgtggagtta aataatgctg ataagtaaaa atttttccgt caattaaact
     3361 gccactagtg aatcctgttc tattggtaaa aagagaatag tctgggtatt catctacacc
     3421 aaatgataaa gattcagcca taaactgttt tttaccagca aattcagcac tgctaccttg
     3481 atggatatgt cctacaaaaa tacctaaaat atcactttcg gcaatcaatt cagcaaaacc
     3541 ttccggaact tcagttacaa tagatggatc ttcccacgac aatggatgat gaaccaataa
     3601 aatcgttccc ttttcagcag gtgtacccaa ccactcttgt aattgaatca tctgtcctat
     3661 cgatattttt ccttcgtgct gggtatcttg agcagaatcc aaggaaataa ttcgtaaccc
     3721 attttgattc gtacaatgga tatactctcc cgtttcattg ctttcaacac tcattccttc
     3781 aaagaaaaca ctgcgacgat catgattccc acgacaaaaa tagtaaggtg tggttgggaa
     3841 atattgatcc aacattttcc gtaagacttg ataatcagaa atcccccctt catgtactaa
     3901 atcacctgtt aatagaataa aatcgtacac tgttaacgag agttcttgta acaatttttc
     3961 caatgccaac tgtggatttt taattgttgg aaatagttca gcagtaaaca agttagtttg
     4021 atagttttct ctaaaatgga tatcagagag atgcaaaaag cgcatggcta ttccctcttt
     4081 cttcaataaa ataatcagga tatcctaaat tttcttgcgt ctgataatca aaaaaatgca
     4141 ttctttcagg ttctatgcgg agattgactg catcaccaat ctgccaagta ggtttttcat
     4201 taaggacaat catttctata ccatcgactt taacggtgta cgcataactt tgacctaata
     4261 gctcacgata cttcacttga ccgtgtaaat cgcttgtttc ttttgaaaag gttacgtgtt
     4321 ctggtcttat gcctaaatac aatgagccac taacctgaag aagttgtttt tgccaagctg
     4381 gattcaatgt taaaaattga ctggctacct ttaatgttcc ttgatcataa gtaactggca
     4441 taatattcat gcctggcgaa ccaataaaat ttgcagtaaa agtattttta ggtttcgtat
     4501 aaacattttt cggcgtatca atcatctgaa ttctaccttc attcaataaa acaatccggt
     4561 cagctaacgt catcgcttca atttgatcat gagtgacgta gacaaaagtt tgtttataac
     4621 gttgatgaat ttttaccaat tcttttcgtg catccaatcg taaacgcaca tccaaatttg
     4681 ataacggttc atctaataaa aaataattac tccgtttaac caccgcacga gccaatgcta
     4741 cacgctgccg ttgccctcct gacaaatctt taggaagtcg atcttggtat ggcgttaaac
     4801 ctaacatctt caaagcttct tccaaacggt gctgaatgtc agctgtctta attttattcg
     4861 cccttaatgc aaaaataata ttctccttta cagtcatgtg aggatataaa gcataatttt
     4921 gaaaaaccat tgcgatctct cgttctccac aaggtagctt atttgctagt tgtgtaccta
     4981 aaaaaagctc tccactcgta attttttcca aaccagcaat cattctcagc aaagtagatt
     5041 ttcctgaacc agatggtccc agtaacacaa ttcgttctcc agcggccaca gctaaatcaa
     5101 tttcttttaa aatggtatgt tctccatatt tttttccgat atttcttaat tcaagcgttt
     5161 ctcccatgat ttgtcctcca atttatttaa tacctgcaga aacaaaagtc ccgataatat
     5221 atttttgaaa aattgcatac aagactaacg gtacaaacat tgtaattaaa ctaatcgcca
     5281 ttgctacggt aaaatttgtc ccaccttcag aactaatata ggtctgcatc gccaacggca
     5341 aagtataatt atctactgtc cgtaaaatta ataccggcca aacatactca ttccaagagg
     5401 taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct agtaagagga aaaacaattt
     5461 tactcatgac ggttgtttgt ttgatgttat ccaatgccgc cacatctaga aggctcactg
     5521 gaatttcccg cattgtttgc gtcaataaaa aaatgcccat cgcatcagca aattgtggta
     5581 ttatcacacc ccaagtagta tcaattaaat taagttttgc aatcaacaaa taattaggaa
     5641 tcatcgcaac agtaaaagga atgaacatcg taccaatgat gataaagtac cagattttct
     5701 tccctttgat tcggcaataa gtaaaactat atgccgcaaa aaaagctacg cctaacttaa
     5761 caaccgttat aattgtagca ataaaaaaag tatttctcgt aattttgata attggaagat
     5821 tttcgaacaa atagcaaaaa ttttccaagg taaacggctg tggtatcaaa ctagtaatcg
     5881 tctgataggc atctttcaat ggcttaaaag aattggccat aataaacaat aatggataga
     5941 taataatgat tatgatgaac ataaataaga gatgtggcaa ccagcgttta attttcatag
     6001 taaaccccct tttccacaaa tttaaatatt acccctaaga aaacagcaaa tattaataaa
     6061 gttaaaactg aaattgctga agattctccg actttataca tttcaaaagc cttttgataa
     6121 gcataataga tcacgtttgc actaccatta ttcggtccac cacgagtgat gatcttaatc
     6181 ggagtaaaca catactgcag accttgaaca atggtcatca ccaataaata tagtcctgtc
     6241 gaactactca taggcaacac aattgtaaaa aaaatgcgcc agatgggtac acgatccaac
     6301 ttagctgctt cgatcatctc ttgatcaatt cccacaattg ctgcaaaaat gacaatgaaa
     6361 ttataaccaa aaactttcca agcagtaata cctgcaatca cagcaattac ccaaccttgt
     6421 aaattcatcc acataggcag tcttaaacca aaatttccca acaagacagc aattggtccc
     6481 gataccggat ttaatatcca tgtaaaaacc atccccgcca ctaccaaaga aatcacagct
     6541 ggaataaaaa ataatggttt atacaaattc tgcccttttt ctataactat tcctaataaa
     6601 aaggatgtaa tatatgggaa cacacagctc agcaagacaa atagaacggt ataggacaaa
     6661 gtattcttca acactttatg aaagactgga tcagtcagca cctttacata gttggcaaag
     6721 ccaacaaatt ctttagtggg ggccaccata ttccagtcaa aaaaactcaa atagatcgta
     6781 taaatcaatg gataaaaaac aaacacgccc aacaaaataa ttgctggcgc cagatacaaa
     6841 taacctacat agcgcttcat ttccgacagc ctcctcatcc ttatgtcaaa agcagacgac
     6901 cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct ttgcttattt gagtaactca
     6961 ttaatttcct tttgagtagc tttcatcgtt tcattgacgg aattacctga taaaatttta
     7021 tcccgcatat ccaacaattt ttgttcagct tctaatccag catctccagg aaatgaactc
     7081 caactgacca tcttatccat ctgatcaatt gctggtatca tcatctgatt ttcagctaag
     7141 aaagctttta atccattttg ggcgtctgca acatctttac gtggtggtaa ataaccagta
     7201 ccttctgtcc atttagctaa agattcaaca ctataaagat attttaagaa atcccaagtt
     7261 gcttcctttt tcttatccgt ttgtgatgta acagctaaca ttgcgccacc agcaggtagt
     7321 ttaacttttt tcccttccca acttggtgac tcaactgctt tagcagtaaa agtactattt
     7381 ttttgaatgt tgtccctttg agcaatagtg gtatagacca tcgcgacgtt accatcaata
     7441 aagctttgca caccttgttc ccacgtagtg tgtaatgccg tctggtcttt taccatcctt
     7501 tgatataatt cataagcagc gataccatct ttagaagcaa aggtagcttt gccatctttt
     7561 attacacttg ccccattact ttctaaaatt gcttgttgtg cccaattatc agcaggttct
     7621 tgaatataca cgccatactt tccagttttt tcttttatta tcttagaaaa agtttgaact
     7681 tcttcccaag tttttggacc attttcatcc aagcccgctg cttttaatat atcttgattt
     7741 aaatataaaa ccggattact aatggagtaa ggaataccca cttgtttacc atctttttca
     7801 gacagtgcca tgatattgtc taaaaaatga tctttaaaat aatcagcatt atccggatcc
     7861 acttcttcta ctaaagattg aggattagta tatttgaaat tttctgcaaa ataatcttta
     7921 aaagcccatc ccacttgtac tacgtccggt atttctttgg aggcaacaga agcttgtaaa
     7981 ttttgcatca agcctttata catatcagga ttatattttg ctacaacctt cactttgtct
     8041 tgagattgat tgtatttatc tactaattca ttcactgttt ttccacctag ggtatccgca
     8101 ttaacatgcc aatactctat cgttacacga ccctccgatt tttttgttgt cttggtattc
     8161 ccactaccac aagctgccaa agagatgaca cttaaaacag acacaacccc tataacaaga
     8221 tttttcttta acataacatt aacttcctcc taatttcttt atggccattt ttctaatcaa
     8281 gaaatattat gaactaccaa tgtaaatttc tagttaattt tctgtaaata attcgaaatt
     8341 tgtgcatttc ggggtacagg gtcactggac atatccatca agctaagaat aaagccccca
     8401 tatggtcatg tatcccatct ccaaatctct taactcctct gttttgatta taaaatagtt
     8461 tattctgtgc tcctttcttc cataaatagg cccatgcaca acgtgaggtt atattataaa
     8521 caccacagcg cgcaaggtac tcaatcgttt ctagttcatt gactgtccat acagaaaatg
     8581 tcaatcctag ctgctgcccc acttcacggg ccaacttagt taaccatcct tcatgaatat
     8641 tgacgatttg aacgctttga taggctaatt tctgtaaggt atcgatcaaa tctgcttcgg
     8701 taatatcccc aagttttttc ccttcgaact gattttcaat attataaaat atatgctttc
     8761 cccaaatcgc agcccaagat aattgtacat agccactaag aacaatttgt ggccagattt
     8821 gataggtttg tgccagcgaa aaaacggcca tttccaatcc ctcttctttt aaatcacagt
     8881 taatggtcat ggttgccttc tcattggcca ctgcttgaaa cacctgctca agggttaccg
     8941 aatctgggac aacttcttac ctgtcatggg ctagtattag cgtaccatct gtttgctgac
     9001 gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa gcgaagaaat gcccagctat
     9061 tctcctccgt attgtcacta ccagagtgtg cagtgatcat ggtcattttg acccctcctt
     9121 taattccgtg attttctccc acacgtccac tataccgatg aagcgttaat ttcttgttaa
     9181 tttcttgtaa attccctgcg ttttttcagt aaactagttg taaatcttgc atgctccttc
     9241 ctacgaactc atgaatcgtc gattttgccc ttctagctag tttcaccaat taagtagttc
     9301 tacattcccc gcttttaacc tactacacct ttttttgttt tcagctattc cttcttcttg
     9361 ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa ggtgttcaat ggcttattcc
     9421 cattgaatga ccgagactta ctatcagatt tctgctcgtt gaaccaagtc tttgatacag
     9481 gatttcttcc gtcatcttct cacaaatacc accttgcgtt tctctagcga ttggtcgatg
     9541 tgcaccctcc cagtggactg tcactacccc tttttgctat gcctagccca caaaaaaagc
     9601 tgtcctcaca gacagccaga ctgtagacaa aagctgttcc aaactcgtaa ctcgagcttg
     9661 gaacagcttt ttatctactt cgtttcgaat ttttgaacaa aaaaataaag gctccttgcc
     9721 tttccatagc caggtggcta atttctttaa attcattgca gcaaacacta gcatcgtatg
     9781 cgtcgcgact ttttccaagc ctcgatactt ggtccaacgc atcccatgct tttcttttgc
     9841 atccgcaaat attcgttcaa tcgtttgttt ccgttttttg taaattgatt tattcagatc
     9901 tgttaggcga agatgttcca cttcatccat cagatctccc caaacatgtc tcgtgatgac
     9961 tcgttgatga tttttactta tggtacattg gtttagcaga ggacagttca cacattcttt
    10021 tggattggat ttgtattctc ggtacccttc tcgagtaacc gttgaaaaag cgagcatttg
    10081 atcttgcgga caaatataac aatcaaaata ctcgtcatag agaaactcat tttttgataa
    10141 tagccccttt ttccctttgg gccttgaata aggcaaacaa ggtcgaaggt tcaaatgagt
    10201 caaaaagtga acaaacttag gtgtcttata cccagcatca gcgacaacac aattgatttc
    10261 aggaaaaagt ctcttcgatt gtttgacgag ctgaaaggcg acttgactgt cat
//
LOCUS       DANKAZ010000086        10192 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000086 DANKAZ010000000
VERSION     DANKAZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            119..625
                     /locus_tag="PWY67_002195"
     CDS             119..625
                     /locus_tag="PWY67_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL2058423.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="PWY67_002196"
     CDS             642..1541
                     /locus_tag="PWY67_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL2058424.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="PWY67_002197"
     CDS             1628..2512
                     /locus_tag="PWY67_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2058425.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="PWY67_002198"
     tRNA            complement(2704..2775)
                     /locus_tag="PWY67_002198"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="PWY67_002199"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="PWY67_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL2058426.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4318..5409
                     /locus_tag="PWY67_002200"
     CDS             4318..5409
                     /locus_tag="PWY67_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL2058427.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="PWY67_002201"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="PWY67_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2058428.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /locus_tag="PWY67_002202"
     CDS             6561..7751
                     /locus_tag="PWY67_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL2058429.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /locus_tag="PWY67_002203"
     CDS             7855..8610
                     /locus_tag="PWY67_002203"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL2058430.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="PWY67_002204"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="PWY67_002204"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL2058431.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2629 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaatatcgg ctctttgtca ataaggactg
    10141 atgagttgtg caaaatcaaa tctgagtcag aatgaaccac attctggctc ag
//
LOCUS       DANKAZ010000087        10168 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000087 DANKAZ010000000
VERSION     DANKAZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            202..1050
                     /locus_tag="PWY67_002205"
     CDS             202..1050
                     /locus_tag="PWY67_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL2058432.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1195..2136
                     /locus_tag="PWY67_002206"
     CDS             1195..2136
                     /locus_tag="PWY67_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL2058433.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2126..4297
                     /locus_tag="PWY67_002207"
                     /pseudo
     CDS             2126..4297
                     /locus_tag="PWY67_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(4409..6580)
                     /gene="copB"
                     /locus_tag="PWY67_002208"
     CDS             complement(4409..6580)
                     /gene="copB"
                     /locus_tag="PWY67_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL2058434.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(6600..8786)
                     /locus_tag="PWY67_002209"
     CDS             complement(6600..8786)
                     /locus_tag="PWY67_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL2058435.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(8786..8995)
                     /gene="copZ"
                     /locus_tag="PWY67_002210"
     CDS             complement(8786..8995)
                     /gene="copZ"
                     /locus_tag="PWY67_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL2058436.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(9008..9448)
                     /locus_tag="PWY67_002211"
     CDS             complement(9008..9448)
                     /locus_tag="PWY67_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL2058437.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(9522..10061)
                     /locus_tag="PWY67_002212"
     CDS             complement(9522..10061)
                     /locus_tag="PWY67_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2058438.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         2866 a         2158 c         1827 g         3317 t
ORIGIN      
        1 acgaatgtac tttaaaaaaa cataataaga tcactttgct tgtatgatgt attcccttcc
       61 attacaatga aataaatagc gattttatga gaaggtgatg tgaaaatgga agaaaaaata
      121 gaagaatcca aacaaaaaat cgatcagttg atataagaat tagaaaaagc ggtcatcagc
      181 aaacgttcaa ctatccaatt gatgatcgtg gccctattag caggtggtca tgtcttattc
      241 gaagatattc ccggtgtagg taaaacgtta ctggtcaaag cactagcaaa agctgtacag
      301 ggaaccttca gcagagtcca atgtactcct gatctgctgc ctagtgatat tcttggtttt
      361 tccgtataca attcacaatc aaaagagttt gaatttcgtc cgggaccgat ctttaccacg
      421 atcttgttag cagatgagat caatcgtacg actcctcgta ctcagtcagc acttttagaa
      481 gcaatggccg agaatcatgc aacgatcgac aacaatacat accctctaga caatcatttt
      541 tttgtcttag ctacgcaaaa tccaatcgag tacgaaggaa catatccttt gccggaagca
      601 cagctcgatc gttttttatt ccgactacaa atcggttatc cttcttttga tgacgaactt
      661 ttgctacttt tggataaatt cgaaaaagtg ttggataact taaatgaagt attgaacagt
      721 tatgaaatag aagaaataaa aacaaatgtg gcgaatgtct ttgtcagtcc cgaagtggca
      781 tcttatgcct tgcagttagt gtcagcaaca agaagtcatg ctgccatcca gcttggcatc
      841 agtccacgag gaagcctttc ctttattcag gcagcaaaag cttatgcttt aatccatggg
      901 agaaattacg tcacgcccaa agatttgcag gatttagttc cttacgtgtt ttcccatcgc
      961 ctgatttttt acgatcgttc attgaaggaa gacgaaaaga aacaaatcat tcatactctt
     1021 gttgcacaaa ttcctattcc tgtgaggtag attgaggtag acaatggaaa aaatcaaaga
     1081 ttatttcatt aattttgtgt tatttctggt atatttcatc attgtgcttt ttgctcttgt
     1141 attcaacaat tctttaggct ggttcttgtt ggcctttttt actctttatg tatcatggag
     1201 cttgatttca ttaagcagct ttctaaagaa actttcggtt acttgtacat tgcagccact
     1261 cgtttatcgc aaaaaaagta tgcaggttat tttttccata gcaaaaaaga gaactttttc
     1321 tttacctctc cctcgtttac aaatcggctt cccagcaatc tttgcagaaa aaaacaagag
     1381 attttgattc ttacaaataa accgcaagaa gtccaattgc tttggcagcc aaaagaaaga
     1441 ggatgccatg aaacattaac tgtcattttt acagcctatg atagtctagg acttttcaga
     1501 aaaagtgcaa aaagagagat ctcttttcct gtgactgttc tgcctgcttt ttgtaagatt
     1561 caagcagaaa aattacagag agttctggaa aaaaaacagc agctgaacgc gtaccaaaga
     1621 ggtcttgatt ttcgggaaca tcgcgcttat cgtccaggtg attctttcaa ccgaatcgat
     1681 tggaaattgt ctgctcatac tcaagagtgg ttataccgtg aatacgatta tcaagaagaa
     1741 gaatgttctc ctcttttttg cttttggggt ttgcccagtc caaaatttga acctctgcta
     1801 gacttgtatt tttctttatg gcatttgaga aaagaaaagc agccggaact tctgatatta
     1861 ggcaaccgcg cctttcatgg caaagacccc ggtcatacct attttgcttc tttggaagct
     1921 ggcgatgaga aagctttttt cgcacacttg aaaaaatatg caaaaaaaca aatcgtcctc
     1981 tttgttcctg agaattctcc tgaagtctca gaagctattg aaacattgtc aaaagatcga
     2041 acggtttatc ttgtttattt cgtagaaaaa caactcatgg tccaagaaaa agacaagata
     2101 tgttcattgc caggaggtga atggattgac gattaaacgg gaaaaatggc tgcgtggcgt
     2161 ttactccttt gtgatactaa gtgtaggtgc tccgatattt gtagtagcta atcaattgca
     2221 aaacagctac cttcttatcc taactattgg tgtcctatgc cttattgtct gctgtattcc
     2281 taaaaaatgg gtacgtttgc tgctttcttt gccagtaatg ataggttgtt tgtttttata
     2341 ttttccttcg aagacatttt ccatattatg gatcttacag tttttttcgt cagtcacaga
     2401 ggaattcttc catctatccc aaggtaaact ttattatttg ccagaaaaga cagctttttt
     2461 tctcataatg ttgctgatct atttatttat tacattgacc gtggattacg actattggta
     2521 cgcacccttt ctttctttga tggcttactt catgatgctt accgtttttc ggaaaaaaga
     2581 tttactgtac gaaatgattg cggtagtaac tgttttgttc gtgtatctgg cagcaaggca
     2641 atttttctcc atccggttgt tgtcgggaaa cactcgattc cagtcattga ctacagttct
     2701 gcttatttgt ttcttaactt ttagcgctat cttgccttta tatcttccga aagtacacca
     2761 atctctcgag gaaacgagtg aaccgatccg agaatatctt aatgatgaag ggctttatga
     2821 cacactccat gagtatcaat taaccggaag tgcaagaaca ggcttcagtg agaatgatga
     2881 acagcttggt ggacctttat atgataatgg cgaagttgtt tttacagcga accaaaaagg
     2941 aaatggctat tggaagatcg aaaataaaaa tcattacacc ggttctggat ggatagaaga
     3001 tcaggacgca cccatccaac caatccaaga attccctgtt gacatcacga gctaccaaac
     3061 cgttatagaa gacaaagaaa acattacatt gaccctaaaa aaacaagttt tcttacctta
     3121 tccttatgga aatactacct tgactgattt ttcattagct agtggacaaa cgctttacta
     3181 tttgcctgaa agccagcgat tcattcctgt cccgaatgaa tcgacacgtt ctgcaacttt
     3241 acaaacagaa aggttagttt ttacgagaga agaattaaaa agcagtcagt taaaagctga
     3301 tgatttagaa aatcagcaga caaatcttca attgccaaaa agtttgccta aacgtattga
     3361 ggacttggcg gtcgaattga ccaaaaatga agcaacttta attcgtaaag tcgaagctgt
     3421 ctaacagtat ttaaaaacaa gcggaaattt ccgctattcc aaaacggata ccgggcatac
     3481 tccaaaagaa cacgattatg tggattattt cctctttgat tcaaaagtcg gctattgtga
     3541 caacttttca acttctatgg tcgtccttct tcgaacactt ggtatcccag ctcgctggac
     3601 aaaaggtttt acaaatgggc tcagaacagg cacagataat caagggaatg aggaatacag
     3661 cattctcaat agtcatgctc attcttggcc agaggtatac tttcctggat ttggctggct
     3721 tccatttgaa ccaacccctt catttgccaa tccaatgggt tcacaaccag ttgctgaaac
     3781 aaccgaaacc gtccagcctg ctatcgaaca accaaatgaa agcgaaaata tggcagaaac
     3841 tatttccagt gagagatctg aggtcattcc atcagaagaa acaacactca caccagctta
     3901 taaaatcgtc ttattcagcc ttggagtgtg tttatccgtc ttgttgttct ttctattgtt
     3961 attcagaaaa tctctgcgct ggacaagtta tcttatctat cttcattttt ttgcttctac
     4021 taatacgatt gcatatcaaa aacttcttaa ccctttggaa acattggtac cgcgcaaacc
     4081 cgcagaatct ttgcaggaat atagcctgcg aatagaacaa gaattcaagc tagatctgcg
     4141 tttcattcga ttaactactc aatatgaagc agaaatatat ggacgacaag cagatccggt
     4201 aaaattcgat aaaaaaacct atttagaaat aacgcatata ataagaaaaa tggtatacaa
     4261 aaaaattctg acaaaaggga gccgcttcaa aaaataagat aaaatttctg gagaatctac
     4321 ttctggaatg ccgagaacca gctgattgag tatcgattac tcgaaaaaaa ggttgtgaca
     4381 cgttttttgt cacaaccttt tttcattatt atctgatttt caatgtcaaa gcatttagag
     4441 ctacaactac cgtacttaac gacatcaaga cagcaccaac tgctggactt aatatgatac
     4501 caattggcgc aagaattccg gctgctagcg gaattgcaag aatattgtaa cctgcccccc
     4561 accagaggtt ctggatcatt ttctttcgcg tttgcttggc tagatcaaga aaatgcaaaa
     4621 tatcttttgg atcgctgttt gtcaatacga catccgcaga atcgattgcg atatctgtcc
     4681 ctgcaccgat tgccatcccg attgaagcac gggctaaact tggcgcatca ttgatcccat
     4741 cacccaccat gattactttt ttcccttggt caaggtattt ctggacaaca gcttctttat
     4801 catctggtaa taaaccgcca taatattctt cgatccctaa ataatctgct actgcttggg
     4861 cagcgttttt attgtcacca gtaagcatga caggagtgat ttttctttct ttcaatgttc
     4921 ggataaattc ttttgcttct gtttttacta cgtcacctaa agcaacgatt gcagctagat
     4981 gattttctaa aatcaggaag ctgatggtat ttccttgttc ttggtaaggt ttcaataatg
     5041 cttgttctac gtttaatccg agacgctcaa cttctttttc attaactatt ttcacttgtt
     5101 gttttttgac tgttgcctct agcccgactc ctgaaagatt ttttagattt tcagcttgat
     5161 aaggcttgat ttcatgttct ttcaaataat tcataatgcc aacagccaat ggatgattgg
     5221 cattcgcttc caaggctcct agatattgaa ggatttcatt tttattgaac gcatcatcaa
     5281 gtacttcgac acccgttaca gtaaattttc cttctgttag tgttcctgtt ttgtccagca
     5341 tgatcacatc tagatcattg gcttgctcca gtgcattgcg gtttttcaaa agtaatccgt
     5401 ttttagctgc tattgatgtg gaacgagcaa caactaatgg gatagctaaa cctagagcat
     5461 gaggacaggc gatgataaag actgttacca tacgttctaa tgcattagga agatctgtca
     5521 agaacaacca ggcgatgaat gccaggatac ccacaattaa tgcgacataa aacagccatt
     5581 ttgccacttt gtcagataat gactctagct tggatttttc tccttgtgct tttcgcacca
     5641 tttccataac cttcgctaga taaccatttt caccagttcc agttacttcg atttcgatcg
     5701 ttccatcccc attgatagaa ccgccaatca ctttgtcatt ggcttgtttt ttgattcctc
     5761 ttgattcgcc agtcacagct gattcgtcaa caattgtttc acctttcaag attacaccat
     5821 ctgttggcat tttatcacca gaccggacga tcaaacgatc accttcgttt acttctttca
     5881 atgaaactgt ctcttctttt ccatctttcg tcaagcgttt cactgattct ggcagtaatt
     5941 ctgctagttt ttgtaaagca ttgctggcat tcgacacagc attcatttca atccagtgac
     6001 ctagtagcat aataacgatc aaagtagcca attcccagaa gaaatccatg acgtgtgtat
     6061 gtggattgag cagatttgcg ataaacgaat agacactata aatatatgca actgtaatcc
     6121 ccatcgcgat cagtgtcatc atagcaggac ttttttgttt caattccatc ttagctccac
     6181 ttaaaaatgg ctgaccgcca tagataaata gtatagttgc taagatcaat acgagccaat
     6241 cagaaccagg aaacgttact tgaaatggaa attccattcc catcattggt gaaaaaagaa
     6301 tgattgggat agctaggata agcgatagcc aaaatttttg tttgaaattc cccatgtgca
     6361 tggaatgatc catgccgccg tgttcgtgat ccatgtgcat cgaatggtcc atttgtttgc
     6421 tgtttgctat atctccatgg ttatgtccca tgtgcatgga atgatccatc tgactatggt
     6481 catgtttcat ctccatctgt ttttcatgag attctgtact ttctttgttt actacttctt
     6541 ttttatgctc atgttttttc tccatatcgt tcccattcat ttttactcca tcccttctct
     6601 tactttttca tgtgtcgatt taagcacagg gagttcaaca atacactgac ggaactaaac
     6661 gccatcgctc ctcctgcaat gattggattc aaaaagccta gtgcggcaaa aggaatacca
     6721 atcgtattat agacaaaagc ccagaaaaga ttttgtttga tttttctcaa tgtcaaccta
     6781 gacaactcga ttgtttgtgc aatggatgcc aaactgctat tcatcagcgt cacatcggct
     6841 gtttccatcg caatgtccgt tccacttccc attgccatac cgacatcagc taatgctaag
     6901 gccggtgcat cattgattcc atcgcctgcc ataccgactt ttttgccatc tttctgaagt
     6961 ttttctacgt aggctgcttt atcctctgga agtacttcag caaagatatg ctttggatca
     7021 ataccaactt gtttaccaat cgtttcggct gccagtttgt tgtctcctgt cagcataaaa
     7081 acatctaatc ctttattctg gagctgcttg attgcttgtt tgacttctag tttgatttga
     7141 tcagccacgg caatcaaccc gatcacttgc ttttcatcag ctaaaaacat gactgttttt
     7201 ccctgctgtt ctaattctaa cgcgtgttct tggtattcat caaatgatag gttcaattca
     7261 attaatcgtt tacgtgtacc cgcaaaatag cgtactccgt taatcgtacc gctgattcca
     7321 gcacctgggt gggctgtaaa atcaatcata tcgtatgttg ctgcttcttg cagttttccg
     7381 tattccacga ttgctttacc tagtggatgt tcagaagctt gttctaaagt ataaaaaata
     7441 gataggactt ctttagaacc gactagatct gtaactttgg gctttccttc tgtaatcgtt
     7501 ccagttttat ccaaaacaat gctgtctaag tgagcagctg cttctaatgc ttctccgcct
     7561 ttaattaaaa ttccatttcg tgcaccaaca cccgttccta ccataatagc tgttggagtc
     7621 gctaaaccta atgcacatgg acacgcaatg actaatacag agacactatg gatcaatgcg
     7681 agttgccaat caccagtgat caaacctgta gctatgagcg tgataaacgc gatgactaat
     7741 acgatcggaa caaaaatccc tgagatccga tctgcgattt tttggattgg ggctttgctc
     7801 ccttgagcat cttccaccat ttgaatgatt tgcgctaata ctgtctgctt gccgacttgt
     7861 gtaacttcca tgtgaagtag accattcgta ttgattgtcc cgccaaagag cgtgtcatcc
     7921 ggattctttt ctacaggaag attttcacca gtaagcatac tttcatctat cgcactgctt
     7981 cctgaaataa ttcgaccatc tgctggaatc tgctcaccag gacggatgac tagttgatca
     8041 ccaacgacta cttcttcaat cgctaacgtt tcttctttcc cgtttcttat cacttgggcg
     8101 gtttttgttt gaagagacat caattgtttg attgcattac ctgttttcgt ttttgctgta
     8161 tgttccaaat atttgccgag caaaatcagc gtgatgatca tactgctgct ttcaaaataa
     8221 agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc tcaatgcaaa ggcagccgat
     8281 gttccaattg ctaccaatac atccatattt ggtgctttcg tcttgattgc atggtacgca
     8341 cctttataaa aacgagcacc gacataaaat tggacaggag cagacagcac taactggacg
     8401 attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc ctaacatcat agcaatcatt
     8461 gacaacatca acggcaaagt caaaaccgta ctcaatataa gatcgaatag catttttttc
     8521 aaatacgctt gcttttcttc tgcaattttt tgtttgtggg cttcatcgta caaaattgca
     8581 ccatagccga tgttttctac gctttggatt aatttttcag tggtcgtccc ttcatatttg
     8641 acacttgctt tttcagtggc caagttgact gtggcattga ccacaccagg ttgtccgttc
     8701 agctcttttt cgatcctagc agaacaattt gcacaagtca tacctgtaat gacgaacgta
     8761 tccattttgg tttctgctgt tgccatcaaa tcacctctgc tttataaccc aatccattga
     8821 ttgcttgaca aatcttttcg gattgaactt ttgtttcatc aaacttgacc gtaccgtttg
     8881 cttttttcaa atttactttc actttttgca cgccgtcaag tgcgcttact gtttcttcta
     8941 ctcttgctgc acaatgattg cagctcatac cttcgattga aaattgttgt ttcatttttt
     9001 cttcctccta ttgatgttgt ttacattcac attgacctgg gatacagtta cattcgatcg
     9061 tatctactgg ttgcttttgt tccagttgtt tcaaaatctc attgatatct tcttgagtca
     9121 aagtggcttc ttctactaga tccgcaatcg tctctccaat cctttttgca cagatatggg
     9181 aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc cgagacagct ggataataaa
     9241 tgaatttttt tccttcttgt tctgtccaga tcacttcttt ttttaccaaa cgacccagca
     9301 aagttttgat cgttgctggt ttccaatcca tcgaatcagc taagatctgc gtgatttctt
     9361 gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat ctcccactct gaatcactga
     9421 tctttactgg cgttttgact ttttccattt atttcacacc tttcgtttac aaatgtaatc
     9481 attatcttat gaatacagat tacacttgta aacaaaaact gtcaattatt tttgctctct
     9541 attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg ttttaaggat gatcagttta
     9601 tttcttgcat tagctacttg gatcagctgc tgtattttcg gttcattgtt ttcttcaaaa
     9661 tgccagataa aagataaaaa ttctaagtat tccctgttta gtttctccga aaatgattca
     9721 ggcatatctg gccgagattt tttgtttatc tttcttttta gagaacgcca aactactcgg
     9781 tagatggcga gataacgcgg cactttaagc caaatgatct tatctgcttc tttaatcctt
     9841 tcctctaatg tagaagtata atttccttca acgatccatg acggattaga taccataaac
     9901 aattgctgtt cttgcaaaaa ccactttttt gcctgaatac tgtagtctgt tgtatgccat
     9961 aaaaggtcta gcggcagtag cggaaagtcg gtgatttctt tgatcttcct tgcgagggtc
    10021 gattttcccg acccactttg tccaatcacg atgatcttca ttcttctccc tcacttctct
    10081 ttggaatttg gttcgatcat accacttata acgccgcaaa aaaacaaggg actgtgacaa
    10141 aagtcgtgtc aaagtccctt gtttcgaa
//
LOCUS       DANKAZ010000088        10086 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000088 DANKAZ010000000
VERSION     DANKAZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(247..759)
                     /locus_tag="PWY67_002213"
     CDS             complement(247..759)
                     /locus_tag="PWY67_002213"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL2058439.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="PWY67_002214"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="PWY67_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL2058440.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="PWY67_002215"
     CDS             complement(3212..3655)
                     /locus_tag="PWY67_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL2058441.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="PWY67_002216"
     CDS             complement(3656..4444)
                     /locus_tag="PWY67_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2058442.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="PWY67_002217"
     CDS             complement(4441..5382)
                     /locus_tag="PWY67_002217"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL2058443.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="PWY67_002218"
     CDS             complement(5559..6146)
                     /locus_tag="PWY67_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL2058444.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="PWY67_002219"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="PWY67_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL2058445.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="PWY67_002220"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="PWY67_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2058446.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="PWY67_002221"
     CDS             complement(9063..9998)
                     /locus_tag="PWY67_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2058447.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DANKAZ010000089        10084 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000089 DANKAZ010000000
VERSION     DANKAZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            162..392
                     /locus_tag="PWY67_002222"
     CDS             162..392
                     /locus_tag="PWY67_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL2058448.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="PWY67_002223"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="PWY67_002223"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2058449.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="PWY67_002224"
     CDS             1803..1979
                     /locus_tag="PWY67_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2058450.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="PWY67_002225"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="PWY67_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL2058451.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="PWY67_002226"
     CDS             2474..2941
                     /locus_tag="PWY67_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2058452.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="PWY67_002227"
     CDS             3026..3910
                     /locus_tag="PWY67_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2058453.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="PWY67_002228"
     CDS             4024..4260
                     /locus_tag="PWY67_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL2058454.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="PWY67_002229"
     tRNA            4348..4428
                     /locus_tag="PWY67_002229"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="PWY67_002230"
     tRNA            4435..4506
                     /locus_tag="PWY67_002230"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="PWY67_002231"
     CDS             4631..4801
                     /locus_tag="PWY67_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058455.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="PWY67_002232"
     CDS             5021..5728
                     /locus_tag="PWY67_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2058456.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="PWY67_002233"
     CDS             complement(5788..6432)
                     /locus_tag="PWY67_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL2058457.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="PWY67_002234"
     CDS             6559..6846
                     /locus_tag="PWY67_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058458.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="PWY67_002235"
     CDS             6954..7274
                     /locus_tag="PWY67_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2058459.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="PWY67_002236"
     CDS             7355..8668
                     /locus_tag="PWY67_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2058460.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="PWY67_002237"
     CDS             8665..9384
                     /locus_tag="PWY67_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL2058461.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="PWY67_002238"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="PWY67_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL2058462.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DANKAZ010000090         9967 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000090 DANKAZ010000000
VERSION     DANKAZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            278..787
                     /locus_tag="PWY67_002239"
     CDS             278..787
                     /locus_tag="PWY67_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2058463.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="PWY67_002240"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="PWY67_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2058464.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="PWY67_002241"
     CDS             2322..3302
                     /locus_tag="PWY67_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2058465.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="PWY67_002242"
     CDS             3424..4842
                     /locus_tag="PWY67_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HDL2058466.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="PWY67_002243"
     CDS             4913..6382
                     /locus_tag="PWY67_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2058467.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="PWY67_002244"
     CDS             6392..6859
                     /locus_tag="PWY67_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2058468.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="PWY67_002245"
     CDS             6895..7767
                     /locus_tag="PWY67_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2058469.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>9967
                     /locus_tag="PWY67_002246"
     CDS             7848..>9967
                     /locus_tag="PWY67_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2058470.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGN"
BASE COUNT         3456 a         1443 c         1986 g         3082 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatca
//
LOCUS       DANKAZ010000091         9919 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000091 DANKAZ010000000
VERSION     DANKAZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            365..1414
                     /locus_tag="PWY67_002247"
     CDS             365..1414
                     /locus_tag="PWY67_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2058471.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1411..2292
                     /locus_tag="PWY67_002248"
     CDS             1411..2292
                     /locus_tag="PWY67_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2058472.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2295..3065
                     /locus_tag="PWY67_002249"
     CDS             2295..3065
                     /locus_tag="PWY67_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2058473.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3344..4198
                     /gene="aroE"
                     /locus_tag="PWY67_002250"
     CDS             3344..4198
                     /gene="aroE"
                     /locus_tag="PWY67_002250"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL2058474.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4376..5539
                     /locus_tag="PWY67_002251"
     CDS             4376..5539
                     /locus_tag="PWY67_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2058475.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5656..6066
                     /locus_tag="PWY67_002252"
     CDS             5656..6066
                     /locus_tag="PWY67_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2058476.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6240..8468
                     /gene="topB"
                     /locus_tag="PWY67_002253"
     CDS             6240..8468
                     /gene="topB"
                     /locus_tag="PWY67_002253"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2058477.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8639..8923
                     /locus_tag="PWY67_002254"
     CDS             8639..8923
                     /locus_tag="PWY67_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058478.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9083..9319
                     /locus_tag="PWY67_002255"
     CDS             9083..9319
                     /locus_tag="PWY67_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2058479.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            9316..9714
                     /locus_tag="PWY67_002256"
     CDS             9316..9714
                     /locus_tag="PWY67_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2058480.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3462 a         1660 c         2097 g         2700 t
ORIGIN      
        1 taaaaaatta aaaaatatag ttgacgaaat tcaaaaattc gattagactt aaatcaattc
       61 ttaaggaaag ggtgacctgt aatgaactta tcaaaacgta tttcagcaga aaacaaaaaa
      121 aattggcgca gctggcgtag ataagttgta ttaatggtga gactttccca tagatacaac
      181 tttttagagt gcatttctaa agcttgtatc tatgctagac atgaacattg actgccgcat
      241 agattagaat ctatgcggtt ttttgtgtaa agacaatgac cgctttggcc attgtctttt
      301 tttgtagact ggatacttgc ctgattataa taaataaaaa gattgacttt gcaggaggaa
      361 aaaaatgaaa aacaaacgat taataacagt tgtagtaatt attttagttt atttagcagg
      421 tgcatttatt tatggaaaag cctcaggcga aaaagaaagt acttcgacga accagacaaa
      481 aaaaacaaca gttgttgggg tattgcaata cgtgagccat ccggcattag atgagattta
      541 tcgaggaatc aaagatggtc tgaaagaaga aggattaaaa gaaggagaaa atcttgagat
      601 caaatttcaa aatggacaag ccgatcagag caagctagcc acaatgagtc aacagctagt
      661 acaatctgat ccagatgcct tgattggaat cgcaacacct gccgctcaat cattagcaaa
      721 cacgacttca tcgatcccag ttgtgttagg tgctgtaacg gatcctgtgg gggctggatt
      781 agtcgcttct ttagagaaac ctggtggtaa tgtaacaggt gtttctgatc agccgcctgt
      841 tacttcacaa atcaagcttg cttctgattt gttgccaaat gcaaaaaaag tcggaatctt
      901 gtatgcttct tcagaggata attcaaaata ccaagtagca caagcagaag aagctgcaaa
      961 aaaagaaggg ttagaatcag taaaatatgc ggtgccttcc actaatgaga ttgcacaaac
     1021 ggttcaagtg atgagccgac aagtcgattt tatctatgtt ccattagata ataccattgc
     1081 caatgcgatg caaacagtgg taaaagaagc aaacaaagca aatatccccg tcattccatc
     1141 tgtggataca atggtggaac aaggtggact agcaacaatc gggatcaatc aatatcaatt
     1201 agggcttcaa agcggtaaga tggcggcaga acttgcgagt ggaaaagaaa aaccggaaac
     1261 gacgcctgtt tatacatttg atcaagggga tacagtgatc aatcaatctc aagctgatcg
     1321 cttaggaatc attgttcccc aatcgatgaa ggaaaaagcc aaaatcatta cagatgactc
     1381 gcagcaagaa acatctaagg aggatgacaa atgatcgtat cagctatttc acaaggcatg
     1441 ctttgggcaa tattaggatt agggattttc atgacttatc ggatcttaga ctttcctgat
     1501 atgacgacag aagggtcctt cccattaggt gctgctgtct gtgtgacagc tattactcat
     1561 ggggtagcgc cgattgtagc tacattatta ggcgtaggtg ctggcatgat agcaggactc
     1621 gttacggggt tactttatac aaaaggaaag ataccggtta ttttagcagg gattttagtg
     1681 atgtcaggat tgaactcagt tattttgttt gtgatgcgaa caccaaatct ttctttattg
     1741 aatcatccga ttctacaaga cagcttccat cgctttgggt tacctgatta ctttgacaca
     1801 gtccttcttg gcttcatctc tttgggcatc ttgattgctg tactgctatt cttcttcaat
     1861 acagatttgg gtcaaggcta tattgcgaca ggagacaacg aaacgatggc tcggtcgtta
     1921 gggataaaaa ccgatcgtat gaaaatttta ggtttaactt tgtcgaacgg tgtcattgca
     1981 ttatccggtg cgctgattgc ccagaacgac ggatatgcag atgtaaataa agggattggc
     2041 gtaatcgtca tcggtcttgc ttccatcatc atcggtgaag tgatcttcca agaattgacg
     2101 ctagcagaaa gattgattgc tattgttgtc ggcagtatca tttatcagct attgatttta
     2161 gtagtaatca aactagggtt tgacacgact tatctgaagt tgttctccgc tatcatctta
     2221 gctatttgtc tgatgatccc gcaattgaag aaagcactga acttaaaaac gggttttgag
     2281 aaggaggcat agtaatgact gatatattga caatcaaaaa tggcagaaaa gtcgtaaaca
     2341 atggaatgaa tgaagagaaa gtactattgg atgaaattaa ccttacaatc agagaagggg
     2401 acttcatcac tgtccttggc gggaatggtg ctggaaaaag cacactgttc aatacgatcg
     2461 ctggtacgct tcaactcagt caaggatcga tctcttttaa agatcagctg atcacaaaag
     2521 attcagaaga aaaaagagca cgattccttt ctcgagtttt ccaagacccc aagatgggaa
     2581 cggctcctcg gatgacagta gcagaaaatt tgctacttgc acaaaaaaga ggtcaaaaac
     2641 ggaaattccg tttacgtaac ttgaaaaatc aaaaagaaga ttttttcaga ctatgtcaag
     2701 aagtcggtaa tggcctagag caacacttgg atactccagc gggtgaatta tctggtggtc
     2761 aacgccaagc attaagcttg ctgatggcga cgatcacagc tcctgagtta ctattattag
     2821 atgaacatac tgcagcttta gatcctaaaa cagctaaagc attaatgcga ttaacaaatc
     2881 agcggatcaa tgaacaaaaa ttgacttgtc tcatgatcac gcatcgaatg gaagacgcct
     2941 tgaaatacgg aaatcgtctg atcgtactcc aaaaagggaa aatcgttcaa gatatttctg
     3001 aaaaagaaaa gcaacatttg acaatgggcg accttttgca gttctttgaa gaagtagtgg
     3061 attgatttcg acagaaaaat tcctttcagt agaaggactt gaaaactcat tgacatcttc
     3121 ctttgattca tcctatactt aaaacaataa acaattaaac atatttgggt gttttgtagg
     3181 atcacgtagt caatgatttt aacaattaaa tagaatattc tttgctctga caagtaaacg
     3241 atgagaaaag aatttgctac tttgccacct agacgtttcg tactttttcg tatcaaacgc
     3301 taagtggctt tttttattga agtaagaaca aaggaggaat aagatgattt ctggacaaac
     3361 caaattagct gggctctttg ctgatccggc ttctcacagt ttatccccat tgatgcacaa
     3421 tactgctttt gaagcaaaca acatcgatgc ggtgtattta gctttccaag taaatccaat
     3481 gaatttgaag caagcagtcg aaagtattcg aacttttgat atgttaggtg taaatctttc
     3541 gatgccgaat aaaacagcgg ttattcctta tctggatgag gtaagccagg aagcgcaatt
     3601 gatcggagca gtgaatacga tcgtgcacga aaagggtcgt ttgattggct ataataccga
     3661 tggtgcgggc ttcatgcaat ccgtgcaaga agctgggatt tcgatcaaaa agaaaaaaat
     3721 gactctactg ggagctggag gtgcagcaaa ggcagtcact gttcaagctg cgttagacgg
     3781 cgctgaggaa atcgtagttt ataaacgaaa caaccaaaca ttcgctcaag taaaagaaac
     3841 attcgaacaa ctcagctatc gaacttcttg taagataaaa gttcgtgact ttgccgatac
     3901 gcatcaatta agaaaagact tgaaagaaag ctgtcttcta gtcaatgcaa cagatatcgg
     3961 gatgggaaag aaaatcaatc agtcaccgct cttagatata agcatgatgc atgagaatct
     4021 agcggttttt gatttgattt atgcacctag agaaacacgt ttattaaaag aagcaaaaaa
     4081 agtaggagca aaaggataca atggacttgg catgttgatc taccaaggag caatcgcgtt
     4141 tgaattatgg acaaaggaaa agatgccgat ccatgtgatt gaaaagctgt ttgattaaaa
     4201 aaggataata gataaagatc ttttattctc gcataaacgg tctcaagaaa agtaatcctt
     4261 tatgtatcga agcatttatt tcgttatttt ttattaaatt atcaaaaaat gataggcttt
     4321 tgtcgattgg ttatcgtatc ctttatttat cagtaagaaa cgaggaggaa ccacaatgag
     4381 tcggatggat cgctataagg acatccataa gaaggcgaaa cctctgaagc gggatacggg
     4441 gagtattttt gctagaagag aacgaaaacg aacgcgtatg gaaaatgagg aagatgctac
     4501 ccgtatttat gaaagagagc agcattcatt tgaacatttg aaccaatacg agcaacagat
     4561 atacagagat gcaccaagag cagaagaaca acaaaaaaat aaaaaatttt tcaatcggca
     4621 aaagaagaac aaaccgttaa aacctcgaag aaagcgatcg tggaaaaaaa tcattctatc
     4681 gattttgctt gttttgatcc tttattcagg tatctcattt tttattggta aatatgtagc
     4741 tgaacatgac acaagcttag aggcagcgac ttcagaaacc tttcatgggc aaaaagcgac
     4801 gaatggtgcg cataatattt tgatccttgg aagtgataca cgcggagaag atgccgggcg
     4861 agccgatacg atcatggtct tacagttgga tggtccttcc cataagccga aactgatttc
     4921 ttttatgcgt gacagcttcg ttacgatccc aggggtagga caaaataaga tcaattcagc
     4981 ttatgcatac ggaggagccg atctagtgcg tcaaacttta gtagaaaatt ttggaatcga
     5041 ttgtcaatac tatgcgaaag tcgatttcaa atcgtttgaa aaagtgatcg atgccttatt
     5101 catgaacgga gtgaagatcg atgcagaaaa agatctgaat ctggatggtg tagatattaa
     5161 aaaaggggtc cagaagatgg atggacatgt tcttttgcag tacgcacgtt tccgaatgga
     5221 tgaacaagga gactttggcc gtgtcagacg ccaacagcaa gtaatgaacg ctattttcag
     5281 ccaattgaag aatccattga atctgatccg tgcaccttat gcagctggta aagctttagg
     5341 atatacatcc acagatgtca ctgcttggtt cttgacgaaa aacctctttt cgattgcacg
     5401 aggagctggt ggcattgatc gaataagtgt gccagtagac ggttcgtgga attatgggca
     5461 aagtgattat gctggcagta ttcttgaaat tgataatcaa atgaatcaat ctgctattac
     5521 agattttttg aataaatgat cataaaaaag atcaagaaga agctgcgcaa ggcttttgtc
     5581 atgaatcaat caaacgattc actcgtaaaa aacgctttct tatggtagat ttaaccaaga
     5641 gaggagctgt ttacattgga tatctatctg aacttcaaaa atgagtcgcg tgaagccatc
     5701 gctttttatg aaaaagtttt tcagactgaa gcatcaggta tcaccacata cggtgaaatg
     5761 cctggaatag aacaaatgaa cttgccagaa gaagcaaaaa atctaatcac gaatgcacgg
     5821 ctgaacattt atgatacatt agttatgttt gcagatacgc cagaaggaat tggtcctgaa
     5881 ttagttgtgg gaaataatgt ttctttagtc atccagacaa gaaatgaaga agaaacgaag
     5941 cgcctatttg aaagcttagc tgagggtggt aagattgaga tgccactgga tgctgttccg
     6001 tgggctcagt tgtatgggat ggtaactgat cgatttggca tcaactggca gattaaccaa
     6061 gagtgacagc taaagatgaa tattttctgg aaacatcaag cagcaaacgg atcatcgttt
     6121 gtttgcttga tgtttttgtg tctatttcga ctatacggta tctttttttt attgccagat
     6181 gcgaaaatat gttccataat agagaagtcc gcaaaaaatt aagtaacgga ggtcagaaga
     6241 tgaagacggt gatactagct gaaaaacctt cacaagcaaa agcctatgcc gaaaccttcc
     6301 aacagcaaaa gcggcatgaa gggtattatg aaatcagcga tccattgttt gctggtgaag
     6361 taaccatcac ttatggtttt ggacatttgg tagatatggt tgctccagga gcttatgaag
     6421 agcgttgggc aaaatggtct ttagaaaatt tgcctatttt cccagaaacc ttccgttacg
     6481 aagttccaat ggacaaacgt gcacaatttt cgatcgtaaa acgagaattg cagtcagccg
     6541 ataccatcat tattgcaaca gatggtgatc gagaaggtga agccatcgcg tggtcgatta
     6601 ttcgacaagc aaacgcattt tctaaggaaa agaactattt acgtttatgg atcaattctc
     6661 ttgaaaaaga agcaatctat gaaggtttta agaatctcca acctggaaag aactattttc
     6721 caaaatataa agaagcgcaa gcaaggcaga acgctgattg gctgattgga atgaatggaa
     6781 gtccattata tagcttgcta cttcaacgaa aagggattga tggcagtttt tctcttggtc
     6841 gtgtacaaac accgacactc tatatgattt atcagctgca agaagaaatc aaacacttca
     6901 aaaaagaaac gtactttgaa ggagaaggtt taattacgac aaacaaaggg cagttttctg
     6961 gaaaaatcgt accaaaaaaa gcatttaaaa cccaagaaga gttaacgaag gaaatagaaa
     7021 aacttggagc gcatttaggt aagcaaaacg gacggattct tgaagttacg aaaaaggaaa
     7081 agaggttgca tagtccacgt ttattttcgc tatctagttt gcagacaaag atgaatcagc
     7141 taatgaaagc aagtgccaaa gctacacttg atgctgctca aggattgtac gaaagcaagt
     7201 ttctaagcta tccaagaacg gattcctttt acattacaga aaatgagcat cagtatctgg
     7261 ttaaacatct gcaaaattat aaaaaatttt taggaataga agaagtacag acaactgaga
     7321 tcaaagcaaa gaaacgatat gtcgatgcga aaaaagttca agaacatcat gcaattattc
     7381 cgacaaagac gattccgact cctgcacgct ttgcgaagct ttccaaatta cagcaagcaa
     7441 tctatttgca agttttgcgc actactgtgg ctatgtttgc tgcagattat atctacgaag
     7501 agacgaccgt aatgacgggc gtacagcaat taaagctaaa aagcatcgga aagatcccat
     7561 tacagcaagg ttggcaggaa atcttacgca tcaagtcaaa aaagaaagaa gctcagactc
     7621 ttcctcctat cgtagaaaat gaagaagcag aagtagaact gaaaagtatg gagaaagaaa
     7681 cacaacctcc aaaaccctat accgaaggaa cgttgatcac agcaatgaaa actgcgggaa
     7741 aaacgctgga taatgatgaa gcgcaagaaa ttctcaaaga agtagaagga atagggacag
     7801 aagctacacg agccaatatc attgaaaatc ttaagcagaa gcattacatc gaagtcaata
     7861 aaaacgaaat aactgttacg ccaaaaggaa tcactctttg taaggcagta gccaatgaac
     7921 cattactgac gagcgcagaa atgactgcgc gctgggaagg gtacttgaga aaaattggta
     7981 aacaagaggg gacaccatct gtttttttag agaatatcaa aaaattcatc ctacatttat
     8041 tggcagaagt tcctaatcaa atcgacaagg tagacttttc agatgagatt agcgggacac
     8101 gtcagataaa agcgatggag aagaaaaacg atcaattagg aagatgtcca aaatgtaaaa
     8161 agggaatagt catgctttat cctaaaatcg ctacatgctt gaatccggaa tgcgacttca
     8221 aattgtggcc aacagtagct aaaaagaagc tgactaaaac gaatctcaga gatttgcttt
     8281 caaaaggaaa gacaagtaaa gttgtcaaag gttttactgg taaaaaaggg aaattcgatg
     8341 cagttttagt attaaaagaa gatatgacaa tcggtttttc ttttccatgg atcgaaaaca
     8401 gtggaacaaa agcagttgat ggaaacaaag aaacatcaag aaaaaataat gaaaacgaag
     8461 tagagtaaat gaaaacaatg aaatagcgga atatttattc cgaaaagtga gaatataaat
     8521 accaataaaa ctaaaaaata tcagtgaatc aagcaaaaac gttggggtag gctgatttta
     8581 tatagatagc aaggagtttt tttatttgaa aaaagtgcat tcatttttaa attatcttat
     8641 gggagcaatc acgattgtgg ctgtgtacca agcatttcta aagtatcctg attacgggac
     8701 gaagttgttg tcattgacgc acaatcaaac agcatattca ccaacgttga ttttatcgac
     8761 tttgactgca ggagctaaaa gcgctactta tttttttcat aaaacgacaa gcatgctaaa
     8821 gcgaaagaaa cagcgaaaac aattgttggt ggtggtagcg gctatgatca ttatttattt
     8881 tctaccagtt attacacaag tatttcatca atattttggt taagaatacg agagtttgtg
     8941 acaaaagtca tgtcacaaac ttttttcttt gtcatttttt cgtaagaaaa agcaaaataa
     9001 aagtatatac attgattaaa agtatatact tggatataat gtacttacta tagaatgaga
     9061 atggtataga gggtggcgaa atatgaaaaa aatgaaaaag tataaaatta gaaaatctgg
     9121 caatagcgat gtaacaacta tcccaccaga agtaaaagaa ttcatgggtg tgcagacagg
     9181 tgacgcaatc agttatgttt ttcaatctga tggttctgta cgaatgatca aagcacaaga
     9241 agaacctgat attgattcat tagttgattc gatcatgaat caatatgaag acgcgttaaa
     9301 agatttggtt gatttatgag gtatctttcg actaaagaaa ttatcaaaat caacgcgaaa
     9361 gttattttac gtcactcgcc aggcgaaatg atcggtgtaa aagatgcaaa tgcattagat
     9421 atggccgtca aacagccttc acaagcagta ttcaatcaag aactctatcc tgaaatctat
     9481 gaaaaagcgg cgattttagc aattaattta gctaaaaaac atccttttca taatggcaat
     9541 aaacgaactg ctcttgttgc catgttgttg tttcttcaat tgaataattg tccagttgct
     9601 ttttctcgtc aagaagctgt tgattttatc ataatgatta ccactagttc attggatttc
     9661 gattcactaa agtccaagat aaccagttat ttacgacaaa ccgcaataaa atgaacgaat
     9721 taaaaacttt atttctcaat tagatcagca tgtcattaag gaatatgctg atttttttat
     9781 gtttaatcat acaaaatagt tttttcataa aaatatggaa aatatggatt aattttgaaa
     9841 taattgattt aaaaggaata caaaagagtg aatagtagga aatagtggtt tagaactagt
     9901 gggctgattt tttatatat
//
LOCUS       DANKAZ010000092         9912 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000092 DANKAZ010000000
VERSION     DANKAZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            201..680
                     /locus_tag="PWY67_002257"
     CDS             201..680
                     /locus_tag="PWY67_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058481.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="PWY67_002258"
     CDS             713..2941
                     /locus_tag="PWY67_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2058482.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="PWY67_002259"
     CDS             3070..4713
                     /locus_tag="PWY67_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2058483.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="PWY67_002260"
     CDS             complement(4786..5331)
                     /locus_tag="PWY67_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL2058484.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="PWY67_002261"
     CDS             complement(5354..5839)
                     /locus_tag="PWY67_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2058485.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="PWY67_002262"
     CDS             complement(5829..6641)
                     /locus_tag="PWY67_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL2058486.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6780..8312)
                     /locus_tag="PWY67_002263"
     CDS             complement(6780..8312)
                     /locus_tag="PWY67_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL2058487.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8421..9452)
                     /locus_tag="PWY67_002264"
     CDS             complement(8421..9452)
                     /locus_tag="PWY67_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2058488.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3297 a         1836 c         1905 g         2874 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc attgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
     6721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
     6781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
     6841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
     6901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
     6961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
     7021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
     7081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
     7141 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
     7201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
     7261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
     7321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
     7381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
     7441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
     7501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
     7561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
     7621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
     7681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
     7741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
     7801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
     7861 gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc atttgaaatt
     7921 tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc taaacgatcg
     7981 ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg cactgcacca
     8041 aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt ataaataata
     8101 actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt caacacactt
     8161 tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga aagtggctca
     8221 tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag
     8281 ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt tgttaagtaa
     8341 tatacaaatt caagataatc gtaacataat cctttatata ataacatagt ttattgtgaa
     8401 aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc taattcgtga
     8461 taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga gaggtatcga
     8521 ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc ataataacga
     8581 tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt gctcaaaatt
     8641 tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac aaaagaattt
     8701 tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat cgcttctttt
     8761 ggtattggta ctttattcag tctgttttct tttctattca gattaagaat ctgcttggtc
     8821 aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc gcaatacaga
     8881 agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct agcttctctg
     8941 cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa aagttctgca
     9001 tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa aagagaaatc
     9061 tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc
     9121 ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg gttcgctgta
     9181 gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata atcttgtttt
     9241 tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct caaataatca
     9301 gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag ggaagaacga
     9361 ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg ctttaacttt
     9421 tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata gtgttcattt
     9481 tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat cttaggaagc
     9541 aaatttttaa ctaagtacta gctaaaagat atgaagtaag aagatacatt tttagttatt
     9601 ttattagtgc agtcattctt ttgatctttt aattgattta tcctcttttt agctatgtgg
     9661 ttacttcatg agcaatgaaa tggatttggt tcgttctaat ggcaggaggc tctttctttt
     9721 atttccgaaa aattagaaaa cattttttca gaatcgattg gctctccatt tattcaataa
     9781 tggtttgtct aacgatattg ttgggataat tcttaaagaa gtattagaaa tagttggtac
     9841 ttcattactt tacatgaaga tttattctat ggtaggagca attatattga gaattaatcc
     9901 ttttttatat aa
//
LOCUS       DANKAZ010000093         9424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000093 DANKAZ010000000
VERSION     DANKAZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            24..1316
                     /locus_tag="PWY67_002265"
     CDS             24..1316
                     /locus_tag="PWY67_002265"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL2058489.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            2052..2915
                     /locus_tag="PWY67_002266"
     CDS             2052..2915
                     /locus_tag="PWY67_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2058490.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(3044..4666)
                     /locus_tag="PWY67_002267"
     CDS             complement(3044..4666)
                     /locus_tag="PWY67_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2058491.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            4915..5436
                     /locus_tag="PWY67_002268"
     CDS             4915..5436
                     /locus_tag="PWY67_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2058492.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(5622..7352)
                     /gene="spxB"
                     /locus_tag="PWY67_002269"
     CDS             complement(5622..7352)
                     /gene="spxB"
                     /locus_tag="PWY67_002269"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL2058493.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            7517..8335
                     /locus_tag="PWY67_002270"
     CDS             7517..8335
                     /locus_tag="PWY67_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL2058494.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(8438..8635)
                     /locus_tag="PWY67_002271"
     CDS             complement(8438..8635)
                     /locus_tag="PWY67_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058495.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            8804..9382
                     /locus_tag="PWY67_002272"
     CDS             8804..9382
                     /locus_tag="PWY67_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2058496.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         2839 a         1730 c         1898 g         2957 t
ORIGIN      
        1 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
       61 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
      121 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
      181 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
      241 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
      301 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
      361 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
      421 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
      481 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
      541 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
      601 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
      661 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
      721 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
      781 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
      841 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
      901 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
      961 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
     1021 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
     1081 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
     1141 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
     1201 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
     1261 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
     1321 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
     1381 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
     1441 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
     1501 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
     1561 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
     1621 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
     1681 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
     1741 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
     1801 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
     1861 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
     1921 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
     1981 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
     2041 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
     2101 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
     2161 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
     2221 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
     2281 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
     2341 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
     2401 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
     2461 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
     2521 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
     2581 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
     2641 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
     2701 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
     2761 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
     2821 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
     2881 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
     2941 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
     3001 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
     3061 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
     3121 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
     3181 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
     3241 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
     3301 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
     3361 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
     3421 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
     3481 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
     3541 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
     3601 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
     3661 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
     3721 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
     3781 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
     3841 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
     3901 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
     3961 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
     4021 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
     4081 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
     4141 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
     4201 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
     4261 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
     4321 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
     4381 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
     4441 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
     4501 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
     4561 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
     4621 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
     4681 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
     4741 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
     4801 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
     4861 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
     4921 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
     4981 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
     5041 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
     5101 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
     5161 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
     5221 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
     5281 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
     5341 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
     5401 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
     5461 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
     5521 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
     5581 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
     5641 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
     5701 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
     5761 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
     5821 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
     5881 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
     5941 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
     6001 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
     6061 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
     6121 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
     6181 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
     6241 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
     6301 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
     6361 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
     6421 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
     6481 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
     6541 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
     6601 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
     6661 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
     6721 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
     6781 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
     6841 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
     6901 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
     6961 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
     7021 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
     7081 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
     7141 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
     7201 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
     7261 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
     7321 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
     7381 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
     7441 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
     7501 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
     7561 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
     7621 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
     7681 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
     7741 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
     7801 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
     7861 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
     7921 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
     7981 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
     8041 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
     8101 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
     8161 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
     8221 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
     8281 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
     8341 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
     8401 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
     8461 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
     8521 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
     8581 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
     8641 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
     8701 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
     8761 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
     8821 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
     8881 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
     8941 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
     9001 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
     9061 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
     9121 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
     9181 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
     9241 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
     9301 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
     9361 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
     9421 aaaa
//
LOCUS       DANKAZ010000094         9360 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000094 DANKAZ010000000
VERSION     DANKAZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(91..759)
                     /locus_tag="PWY67_002273"
     CDS             complement(91..759)
                     /locus_tag="PWY67_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2058497.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            893..1180
                     /locus_tag="PWY67_002274"
     CDS             893..1180
                     /locus_tag="PWY67_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058498.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1596..3134
                     /locus_tag="PWY67_002275"
     CDS             1596..3134
                     /locus_tag="PWY67_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL2058499.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3131..4096
                     /locus_tag="PWY67_002276"
     CDS             3131..4096
                     /locus_tag="PWY67_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2058500.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4083..5405
                     /locus_tag="PWY67_002277"
     CDS             4083..5405
                     /locus_tag="PWY67_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2058501.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5468..5572
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5646..6389
                     /locus_tag="PWY67_002278"
     CDS             5646..6389
                     /locus_tag="PWY67_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2058502.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6404..7396
                     /locus_tag="PWY67_002279"
     CDS             6404..7396
                     /locus_tag="PWY67_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2058503.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7408..8145
                     /locus_tag="PWY67_002280"
     CDS             7408..8145
                     /locus_tag="PWY67_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2058504.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8135..8857
                     /locus_tag="PWY67_002281"
     CDS             8135..8857
                     /locus_tag="PWY67_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2058505.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8941..>9360)
                     /locus_tag="PWY67_002282"
     CDS             complement(8941..>9360)
                     /locus_tag="PWY67_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL2058506.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2713 t
ORIGIN      
        1 ttttttataa agaagatttt actgaataat atcattttag tttacataat gatatttctg
       61 taaattaaat aaagtagtga aaaataagaa tcagtgctct aactgttcaa gaggatcaag
      121 tggttcaaat gtttctgtag tctgttttgc ttccaattgg ggcaaaacag atccaatttc
      181 cagatacttc actttattca atgtataaga tagaatagaa cagctaagta atgtatacag
      241 aaaatgtgtg agatccaaat aaagcaagga gacaactaaa ataccaaaag taatcaagaa
      301 aagacttttg ccaatcttaa tatgaaaata tttttcaatg gcttgataaa tgatatctgt
      361 tccgcctgtg cttccgccga atcgaaagac gagaccgcaa ccaatgccag aaagaatacc
      421 tgtcatcaaa ctattgatca gtagactatc tacagcaaaa tgtatatgaa ggctctccca
      481 taaatgtaaa aagaaaacta gcgcaaatgt tccgacgatc gtccggagaa aagcgtgttt
      541 tccaaaaaga aacaatgaga taagcaataa aggtaaatta aaaagcagtg aagtgacaga
      601 aggagcgaat ccaaaaagat tatgcaaaag caaagcaata ccagtaaccc caccatctgc
      661 cagcttagat ggaatattga ttcctgtcac agctaatccg tagattcctg cgcctaaaat
      721 gattccgcac aattctgtaa ataaatttaa ttttttcatt tctagcctct tttcttttga
      781 aaattttcag caacttcttt attatactga cttatactct tacttcaaga ggaaaatgaa
      841 aaaaaattat ttttggctgt ttctttttat ttatcaaata aggagtggaa aaatgcgagc
      901 agcagactta ttgacaattc ttacaaaatc acccaaaaaa acagagtatc aaggagtttt
      961 tttggctcaa gaacaaaaac tggtgcctgt gacaatggtc cagataaata aagcaaatca
     1021 atttatcttt ttatttgaac acggcaaagc accacttgct atgaaagaca tctttctttt
     1081 tttgatgaag aaccgtgaga aagctgttta ttatcagtca aaagaagaac tcattcctct
     1141 ttatggagtg aaagagatag aaaataaagt ggtgatataa gtaaaaataa gaaaacaaga
     1201 cgtttatgat gtcaaaacaa ccatttttta catttttggc aaaaaagtgt catgatacag
     1261 tataatattc gttgaacagg ccaataaagg tgttctcccc cacgagattt ctactttatt
     1321 ggctgtttct ttttattaaa ataaaagttg tgtgtgtaaa aaagatatag ctgattccaa
     1381 gtgacaattt cgtatagatt tttatggtat tcgtagtgaa aaaacgagaa ccaaaaagtt
     1441 gatccgactt tgttgcttgg attctttttt tgtcagcata tttagaggta tttttttctt
     1501 ttttttgttc gtgctgtaag gttaaggcaa gacaagttag ttgggaaaaa ggaaagaaat
     1561 gtttatactt aaagaataac agaaaggaag gtcgcatgaa taattttata caaacattaa
     1621 gtgaaagaaa aggcgagttg ctggaagctg catttcaaca tctgtccatt tctctcattt
     1681 ctttgctgat tgcagcactg attgctattc ctttagcgat ttgggcagcc aatcataaaa
     1741 aaatggcaga agtcttattg cagatcacgg gtgtgctcca aacgattcct tcacttgcct
     1801 tattaggttt acttatccca tttgtaggga tcggaactgt tccagcattg attgcacttg
     1861 ttatttacgc tttgcttccg attttccaaa acacgtatat tggtctagct gaaattgacc
     1921 cgtctattga agaagcagcg gtagcatttg gtatgtccag aatgagacgc ttgctgaaag
     1981 tagagcttcc catcgcatta cctgtcatta tctcaggtat ccgaactgcc ttagttttaa
     2041 tcatcggtac ggctactttg gcagcattga tcggagcagg tggattaggg acatttattt
     2101 tgttaggtat tgaccgaaat acacctgttt tgacattgat tggtgcgatc agttctgcct
     2161 tgcttgcaat tatattcagc ggactgattc gctggttgca gcataagaaa cctcgatatt
     2221 ccatcattac tttagcagca gtggtgtgtt tatttggcgg cgtttcttta tatcagtctg
     2281 ccttgtttaa aaatgaaacc atcacgattg ctggaaaatt aggagcggaa cctgaaatcc
     2341 tgatccaaat gtataaagaa ttgattgaag acaacacaaa aacagaagtt gaattaaaac
     2401 cgaattttgg caagacgagt tttttgttca atgcactgga tcaaggtcaa atcgatatct
     2461 atcctgagtt cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa gcaatgaaaa
     2521 gtgctgattt gacaaaagaa aaaacatatg acgaagcaga agaattattg aataagcaat
     2581 tcgatatgac attattaaaa ccaatggctt accaaaatac gtatgccttg gcagtcaaga
     2641 gatcttttgc gaacgaacat caacttaaaa agatttccga tttggcagct atctctgatc
     2701 agatcaaagc gggattcaca ctggaattta ttgatcgttc agatggatat aaaggaatcc
     2761 aagaagcata tggcttgaat ttttcaagtg tacaaagcat ggagccaagt ttgcgttacc
     2821 aagcaatcaa taatggagat gtcaatattg tggatgccta ttccacagat agccagctca
     2881 aggagtacga tctagtgact ctagaagatg atcgagggtt attcccagct tatcaaggcg
     2941 caccgctgat gaaaaaagat tttgctaaag aacatcctga aatcgttaaa gcactgaata
     3001 aattaagcgg aaaaataacg gaggatcaaa tgattgatat gaattaccaa gtcaatgtcg
     3061 aaaagaaaaa gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga ttagtaaagg
     3121 aggaagatca gtgactaaat taatcgagtt caaacatgtg caaaaaaagt atgatggaaa
     3181 gtatgtcatc gatgacctaa atctggcaat caaaaaagga gaaatctttg ttttggttgg
     3241 tccttccggt agtggaaaaa caactacttt gaaaatgatc aatggattat ctaaaccatc
     3301 agcaggagac atctacttca aaggaaaatc gttaaatgaa tataatctgc agaaaatgcg
     3361 gtggaatatg ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga cagtcaagca
     3421 aaatattgaa gtcatccctg aaatgttagg ttgggaaaag cagaaaagag cagaccgtgt
     3481 agatgaactt cttcaaaaag ttggcttatc tcctgatatt tatcgagatc gtatgcctag
     3541 agagttgtct ggaggagaac agcaacggat cgggatcatc cgagctattg ctgcaagccc
     3601 agatgtgatt ttgatggacg agccatttag cgcattagat ccgatttcaa gaaactcatt
     3661 acaagaattg gttttgtcct tacacgaaga attgggcacg accatcgttt tcgttacgca
     3721 caatatggaa gaagcaatca agctaggtga tcgtattgca tttatgaagg atggagaaat
     3781 tatccaatgt gacacaccag agcagttatt gatgaatcct aaaaacgact acgttcggca
     3841 tttctttgat gaaccgaaac agacgaaaga gtggcgagta gaagatttag tcgtcaatgg
     3901 atatttctta aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg atacgcctat
     3961 gaaagaagta tattcgcttc tttcggtgta tccaagcatt gtgatagtag aaaagcaaag
     4021 aagtattggg tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag aggaggaagg
     4081 aaatgaaaac atatgatgca atcatcatcg gttcaggtgt gagcggctta agtgctgctt
     4141 atggattgaa agaagcaggt aaaacagtac tagtggtcga agaagatcta tggggcggga
     4201 cttgtcctaa ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta gaggcacgta
     4261 atcgggtaaa acaattgagt ggaaaaggtt ttaacgagat cccaactgcc aactgggaag
     4321 aacttcaaaa attcaaacga acatttacgg atccagtccc agaaagccga aaaaaacaat
     4381 tagcagaagc tgaaatcgat catctttcag gaaccgctcg ttttttggat gattcatcta
     4441 ttgaagtcaa cgaagaagtc ttccatgcgg attacttagt tttggcaacc gggcaaagac
     4501 ctacgatctt accagtggag ggaaaggaat acttgaaaac tagtgcagac tttttatcat
     4561 tgcctgtact gcctaaagaa atcattttta tcggtggtgg ttatattgct tttgagctag
     4621 ctacgattgc caatgctgca gggagcaaag taaccattgt ccatcataat caacggccat
     4681 taaaagaatt tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa gcatctggca
     4741 ttcagtttgc ttttggtgtt gaaacccaaa agatcatttc agaaggtact cgctatcgtt
     4801 tagtcggaaa agagacggaa ttagtagcag atatgatttt ttgtgcgact gggagacagc
     4861 cgaatacaga atctttagca cttgagcaag cgaatatcgt ctttgataaa cacggaattg
     4921 cagtcaatga ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga gatattgttt
     4981 caaggaaaac cccaaaattg acaccagtcg ccacttttga aggaaattat gtagctaagc
     5041 ggataacgga tgcaacaagc gaacctatca aatatccgat tatacctacg atcgtctacg
     5101 ccagtcctaa acttgctgaa gtaggagtta caaaaagtca cgcctcttcg tcagatcaag
     5161 tggtagagat ggatcttacc agttggttca cctatcatcg tgtcaatgaa ccagttgcta
     5221 aggctgaatt gacctttgac cagcaaaatt atttgattgg tgctgcagtg attagcgagc
     5281 aagcagacga attgatcgat gatttgacac tcgtcatcaa tcaaaaattg actaaaaaag
     5341 agttggatag ctatatcatg gggtatccga cattggcaag tgatctctcc tatttattga
     5401 aataatcagc atgataacta aagcgcttgt tgctttttcc ttcttgtgct acactttaaa
     5461 aaatgaatac ctgtttctgg ggatgtcgta gacgacatga gagtaagttg tgaacttaga
     5521 cccattgaac ctgtcagtta atactggtga agggagaaac atagcagacg cttgaagcgg
     5581 gttactatgt cgtgcccatt tcaagcgttt tttgttattc atattagaag aaaggaggta
     5641 acaaaatgaa gaaaaatctt tatccagcag tcagtatggc attcatttta attatttggc
     5701 agattgttgt catagtttgg aaaatacctg cctttatttt acccggacct gctgctgtat
     5761 tttctgcatt agcggcagat gcacaagtat tactttccca ttctgtcacg acattaaatg
     5821 aagcagtgct tggtttggta atagctagcg ttctttcttt tattactgcc ttggcaatgg
     5881 attattggaa gtttttagaa atgtcttttt atccgttact agtgatttca caaatgttgc
     5941 caatcatggt gttagggcca ttacttaccc tatggtttgg tttcggtatg cttccaaaag
     6001 tgatccttgt tgtcttgata agctattttc cgatcgtagt ttctttttcg gatacattga
     6061 ggaaagtatc aaaagagcaa atcatctttt tgaaaacgat gggggcagat acagggaaga
     6121 tttatcgaat atataaaata ccagcgggtt tatccggctt cttttccggt ttaaaagtgg
     6181 ctgctactta ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct caagcaggct
     6241 tagggtatta catgatacgc gtaaaaaaca gctaccagat cgataaagtt tttgctgcga
     6301 tcatttgtgt cattctattg agtttattgc tgaatggcgc ttgttcatta ttgaaaaaag
     6361 ggtattatcg tatgctttat attttttaag agaaggggat caaatgaaaa aattatggct
     6421 tttattaccg ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa caagtagcaa
     6481 acaagaaata aaggatttga aaaaagtgac acttgttttg gattatgtac cgaatactaa
     6541 tcatactggc atctacctag caaaagaaaa aggctattat aaagaggctg gtttaaatgt
     6601 ccaaatcatc gaaccaggag ataattcgac aagtattggt ctagtaggtg cggacaaagc
     6661 acaatttggt gtaagttatc aagaagatgt cacttatgct cacgcagatg gacagaatat
     6721 accagtcaaa gcaattgcta cagtgatcaa acataatact tctggattcg ccaccctatc
     6781 agacagtaac atccattcac caaaagattt tgaaggaaaa acatatgcag gttggcaaag
     6841 tccaagtgaa gaagctgtct taaaagctgt gatggaaaaa gacggcgggg acttttcaaa
     6901 actgacaatg gtaggaagca atggagaagg gccagaaagc ttaggaaaat cctcagatat
     6961 ccaatggtac tttgaaggtt gggatatgat caaagcaaaa gaagcgggta ttgaactaaa
     7021 ctatatccca ttgaaagaat tagatgaacg gttagattac tatactccgg taattattac
     7081 gaatgatcaa ctgatcaaaa gtgatccaga attggtacag tcttttatgg atgccacgaa
     7141 aaaaggctat caggaagcta ttaaggatcc aaatgacagt gctaaactgt tgcaaaaata
     7201 tgcaaaagaa aatgatcgga cgtttttaga agaatcacaa gcattcttgt caaaaaatta
     7261 tacggatgat cctgagaatt ggggattgat ggaagaaaaa gtttggcgca attatacagc
     7321 ttttatgcag gaaaacggat tgatcaagca agatgtacct tcgcaagaac tattcactaa
     7381 tcagtttata aaataggagt gatagatatg tcaatccaaa tcagccactt gaatgtagtc
     7441 attgaaaacc tgaaaatatt agaagacatc catttcacta tccctaaaga tacctttaca
     7501 tcgattgtcg cgccaagcgg agccggtaag tcaacattgc tgaaaacttt aacaggagtc
     7561 cagcctatga cttctggaac gatcaaagta gacgatcaaa aagtaactgg tctccataca
     7621 gcatttagtt atatgccgca agaagatatg ttacttcctt ggctgagcgt ctatcaaaac
     7681 gttacgttat atcaaaaaat caatcatcta acaatcaatg aagaacaagt aagggattat
     7741 ctggatatct ttgggttaaa cggctatgaa cattttttgc cggaacaatt atctggcggg
     7801 atgaagcagc gaacggcctt gttacgaaca atcatgaatc cttcttctta cttgcttctt
     7861 gatgaaccat ttggagcgtt agatgctatg accagagggc agatgcaaga ctggatgttg
     7921 aagctgccta aaaaagcaaa gcggactact ttacttgtga cgcatgatat cgaagaagca
     7981 atttatttat cagacaggat tcttgtgctt tccgcccgtc cggcacatgt gattgctgag
     8041 attcaagtgc ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc agagttgaag
     8101 caaacgattt atcagttatt ggcaggtgaa ttagatgtta agtgatgctc attgtcattt
     8161 agatgggagt cagagtttag cactccttca gcaggaacac acgattttga cgatcatcaa
     8221 ttgtgattcg ccagaagaat ggaaggaaaa cagacagctt gcagcaagta agactcaagc
     8281 cttaagttac ggtatccatc cttggaaagc agatagctat acgtttgaac aagttgagcc
     8341 ttttttgaaa aaagcaagga tcatcggtga aatagggtta gataatattt ggacgaatgt
     8401 accgatgact acacaaaaaa aagtatttga gcgacagctt gcttttgcag ccataaatga
     8461 gaaacctgtt gtactgcaca caaaaggatg cgaaaaagag atcttaaatt atatccaagc
     8521 ttaccctaat cactatttga tccattggta ttcttctttg gactatcaac aagactatat
     8581 tgatttaggc tgttatttca gcattggtct tgatttgaaa aagaatccag cagtctggca
     8641 actagctaaa gcagtcccaa tcgaccactt acttattgaa acggatggac tacatgcagc
     8701 agagtgggct ttagggggga ttttttcaga aaaagattat gtctcactgc ttgaagaaca
     8761 gttaaagaca atcgctgaga tcaaagagct atcagttgaa gaagttaaaa aaaatactta
     8821 tcaaaacttg gaacgtttca ttagattagg taaataaaaa agagatgtga cacaactagt
     8881 gtcgcacctc tttttggctt tctttgtata aaagtaaact gtttgattaa taaatgaatc
     8941 ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct tcccaagaac cataacgtga
     9001 agtaacatat tgttctgcta ctctttcttg gtttgcagca gagtagtcac catttaagta
     9061 agaagaatct aattggtaac gtccgatgta acgtccgttc gttgctgtgt aagaaccgct
     9121 tgattctttt tgtgcgatcc attcttttgc agagcttgtg cttgctggag tagcagcagt
     9181 ttctgttaca gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt tttccgtttg
     9241 aaccgtttct tgtacaacag gttgttcagc ttggactggt gcttcttgaa cggctgcttg
     9301 aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DANKAZ010000095         9254 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000095 DANKAZ010000000
VERSION     DANKAZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..289)
                     /locus_tag="PWY67_002283"
     CDS             complement(<1..289)
                     /locus_tag="PWY67_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL2058507.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEEL"
     gene            complement(279..2453)
                     /locus_tag="PWY67_002284"
     CDS             complement(279..2453)
                     /locus_tag="PWY67_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HDL2058508.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2469..4811)
                     /locus_tag="PWY67_002285"
     CDS             complement(2469..4811)
                     /locus_tag="PWY67_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058509.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4833..7376)
                     /locus_tag="PWY67_002286"
     CDS             complement(4833..7376)
                     /locus_tag="PWY67_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2058510.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7386..7784)
                     /locus_tag="PWY67_002287"
     CDS             complement(7386..7784)
                     /locus_tag="PWY67_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2058511.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7865..8149)
                     /locus_tag="PWY67_002288"
     CDS             complement(7865..8149)
                     /locus_tag="PWY67_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058512.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8173..9171)
                     /locus_tag="PWY67_002289"
     CDS             complement(8173..9171)
                     /locus_tag="PWY67_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2058513.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2627 a         1990 c         1448 g         3189 t
ORIGIN      
        1 gaagttcttc ttgtcttggt tcttttcttg tttcaattcg atatacattc ctagagaatt
       61 ttttgtcgta tctttccaag aaagattcaa cttcttggca ttttttacaa tcatttcttg
      121 ctagatacac aaaaaatgat tgattcgttt tttgaagttc tagtacttct tctaaattac
      181 tattgattag ttttccattt gattcttcgt tagtttttat cagtttaaaa caaccaaata
      241 agaggaaact aacaataagt atcctaacaa taactttctt atttcgcata ttgtttctcc
      301 aaatttggtt tccgaatata gacgactcgc ggtggttcct ggacgtcttc agctgttcga
      361 tacaccacaa attcgcctgt tctcaatgct cgaatgcgat tcggattgaa tttaaagcgg
      421 tctacgtctc gtttcgtgcc tctttctcct gtccaatcaa cctttcccat atcagaataa
      481 cctgcttctt gtgtcgtcat ttccgttaat tcaatgtctt cttctgtccc cattaaatct
      541 gcccaataat caatttctgt cgggtcattt gtcttcccaa taaagaacgt attcacattc
      601 ccaatcaact gttttcgaag gacaggatca attctatcaa tgtccgcaag cgtttgaggg
      661 gcatacgccg catgcacccc agcgcctcgt cccttattga ctaagtctat aaggttatca
      721 tttagataag aagctggttc gtcaaatatt actaggactt tcgagcgatt ggctgtttct
      781 cctgcttgtt tatacacttc tgacgcaaag aaattaatat ctcccacaag catttgagcc
      841 aatgtccgaa taaactcact ataaatcaat ccgtttaacg agatataaac aatctttcct
      901 tctttaacaa cttcttgaat atctaattct ctttttcctc cagaagtatc aaagagttca
      961 ccaagctcac tatccaataa catattgata ttggtctgta aattctcaga agtcgcatac
     1021 aaataaaacg ggttcttctt atgttcgtaa cgaacaaaca accgtttaaa caacgtttgc
     1081 tcgatttctg tcatgagaga aatgttttct tttaataata gataatacgt ttctaagtca
     1141 acggttttac gatcatgttc aacgattcgc tcttcactta actgttccac atcggcaaaa
     1201 tcacgttctg ttttgtccat cgcttcattc tttttcgttt ttttcttcgt ggttttcttc
     1261 ttttctacga ccactttttc tttaaataaa tctggatttt tcaactgaat acgatccgca
     1321 aacaaatgta agacgtttcg tggcaataaa taatgttgta accaaactag atttctttca
     1381 attttatggc tcttttggaa ctcgtctaaa agctgaatcg ttacctgtaa taacgctttg
     1441 gctgctgtcg aataatacac gctttctgtt tctgctagat tggttaattt atctcgaatg
     1501 gcaatatcat tgccattttt tacaggatta taacgcaaat cgccaccgtc cacaaagact
     1561 tcaatcgggc gattgtatgc ctctgccact tcttttaacg cttgtaaagt ctctcctgct
     1621 cctttcccat cgactaagat aacggggata cgaaacttac acgcatgttc tgctagtaat
     1681 tggatcaccg tcgtttttcc tgaacctgta gtcgctggaa taatcacatg ttggttcaat
     1741 tcactaaagt tcatcacata agggagccca tactcatctg ttcctaatag aatcactttg
     1801 gctaatgaag ccagttgttt attccgcaaa gcaacgtctt ttatgacaat tgctacctgg
     1861 tacgcttgct gatactctgc tgtatatttt tgggactcct gaaatttatt cttccgacgt
     1921 tccttgtatt ctttactatt tagcaaagct gtttgccgtt ctttctttga aacaactttt
     1981 cttttttcaa taaaagacgc atacgcaatc aaacaacttc ctgcacctgt cccacctaaa
     2041 aggagggcaa aaatcgtttg tccattaaac tgcaacgcat gagaaaacaa gtttaatgga
     2101 aataataagt aacacgacaa ataccctcta ggaagaacaa aaataaaaca aaatagattt
     2161 aatagcgtaa aacctaacgc aaaaataaaa aaaagagagc ttcttcgttt taagaagctc
     2221 tcatactcaa agtcataaat atgtgcacgt gtctcttttt tatctatcat aaagacaatc
     2281 gctaaaggaa tactgcctac aataaaaaat ggatatagac aaacacctaa taaagtgacg
     2341 ataaaataaa gttgttgaaa ccgacttttt ggcgtttctt tttcttgttt tcttttgcga
     2401 tcgacagaaa acatttgctc aaaatgtgtc ggtcgttttg gtgcattcat catactatcg
     2461 atttcctcct atcgttttcg ttgtttcatt tctcttcgat aaatcggttg tttttctcgt
     2521 tttcgtttca tttttgcttg agttgctcga aactcactcc gtgtcttcgg tgcttgtttt
     2581 ggtgattttg tttgtttcat ggttgagtat gctttagata cggttgaagg cgctttggtt
     2641 tgtgctgtta ctcgattgac tgctgacata tgttcccgaa ccccctctgg acgtcgaaca
     2701 gatttcggtg tggtagaacc tgttgctcgt ggtacattcc cttttcttag tggtttttgt
     2761 accgcatgtt tttgtcctgc aactgtccgg gttttcatcg aacttgtttt tgttacggga
     2821 gtaagtgcct ccttccgacg acgacttgtt tgtccctgac gacgttttcg atcgttcaat
     2881 tctgttctag gcgtatgctt ctggccataa gacttgtgtt tttgctttgg attcgctaat
     2941 gacaccacca ttgcagaacc ctgattgcga tacggactac gagacgcacc ttttctactt
     3001 ctttctctta gactaggatt gccttgcaat tctcgttgta ataaatcggc tgtttttctt
     3061 gcctgtttct ccattgctgt tcgttgcata cgttcttgac tttgctcttc tgcttgtttc
     3121 gcttgatcat gcaagtctct tgctttttgt gccatacgtt ccatacgatt tggtgcttta
     3181 tgacgaggtt gttgtgcgga ttcacgccga aaagcacttc gtgtggcttg tttcgccgtt
     3241 tgtggcatac gaacgtctga gccaccccca cgttttcgtt gacgttcttg tctcttccgt
     3301 gcgttttctt tttctttttg tttctgtgct tttctcgctt ctttgcgttc tttcaaccgt
     3361 tgtttggctt cccttctcaa ttttgcttct gccccaaatg gatttcccgc aaaagctaac
     3421 gctcctcgaa cacccattct tccagtaaat agactgccta aaaagcctcg gaacaaataa
     3481 attcccaacg gcgcaaacaa ataaacaatg atcaagagaa ttttttccca tggatcgtct
     3541 tgtgtactac ttaattggaa cccgtaacta agaaaagaag aaaaaaggat acatgcaaaa
     3601 cctgtcattc cttttaaaaa cgtcaaaata ccaaaaactt tggcatagtt accaaacaca
     3661 ttcattgtga caagaaacaa tcctaaaaaa agtaagaacg gcaacatcgg tactaacaat
     3721 agttgcatag ttccaataat caatcgaaac atacttaaga caaaataaac aacggtttga
     3781 atcagattca cgacaaaata aaacaaggca aatgctgtat ttttccaact attgtaatat
     3841 tgtatgttct tgtttttcag tgtatctgct tcatactctg tgactttgtc atgaattttc
     3901 tcccctattt ttgtacctaa atcattgtcc aataaaagat tgattcgatc atattctttt
     3961 ccagcatatt tgactttctt ctcacgaatt gtgtctacat tggttgtgcc ataatttaac
     4021 aacagatatg gttcataaac attactataa aagacccgag ccgccatcaa tttgcctgcc
     4081 gtttccactt ttttatccgc tgttacctct tctgtttctt ttgtttcata atttgtttct
     4141 tcttcaggtg ttaacactgg attgaccttc acgaacgccg tttccactgt agaatctaaa
     4201 gaaaaaagaa catcaaaaag attcttattt gtcccagaat cttttaatac gcttaaccca
     4261 gtaaaaataa ggatcgtcat aagaaagaca cggagtgctt gtttcattcg agcctctcca
     4321 gcatatttga ttgccatcat aattgccgaa aaactgattc caatagttcc tgcaatactt
     4381 aacatattgt ccgcaatttt ttgtgttaag ttttgaatgg gttctcgaat ggcggaaata
     4441 atatctaatt caaagagaaa ttcaaccatt gaagcattga attctcctaa catttttacg
     4501 ccactccaaa caaaattttt catcactcct gcaacattgt acgatagctt ttttttaagg
     4561 ttcattgact catagtcatt ggtcatcaat tcaaacgaat tctttttgta atttttatac
     4621 aattgatcca catcagtcgt tacttctttt acttctactt tatcgttggc attctctcct
     4681 tcagaacgat cggtttcagt gacacccgca ccatcattca tttgatcggc agaaacagta
     4741 gccccttgta gacagaaaaa aagaccccct aataaaagga gcgtccacca tttcagagat
     4801 ttttttcgca aaaaaaccac ctcttttttg ttttatcgtt gtttttcatt ttctaacatt
     4861 gcttgaattt tttcatcttg attttcagta gaagaaacag ccaataacca actatcaaac
     4921 aacacatcaa tggcaatcgg ttgatttctt cccaaatgat cctggaataa acaggttcct
     4981 gtttgtaatt gttccatcat ttctaaattg gctgaattca cttccatatc aaagaaggac
     5041 aatagtttct ctcgtgcttc tttttgtttc ggacgaaacg caaatttgta agaaattaac
     5101 tcttttttat cttcttgatc gtaatcttta aacgcttgca caacaaggta aatatccgta
     5161 ttattcgcac gtccttttcg caagctatca tcaatcaaat agcttccttg tgcggtatct
     5221 tcataccctt ttgcctcgtc ataaataatc gcagcgtctt cttctttgtt tgtagaaaag
     5281 atatacgtaa acttcataat gacttccata attgccattc ctgcaagttg ttcgctattt
     5341 aatcgacccg aatcaatctc tttttgtgtc ggaatgcgta ttccttgtgt ccctaacacg
     5401 ttgatttgtg aatcaaaacg tatcggcgta ctgtgctcat tcccaatgag aatctttcct
     5461 aaaccaacgc tatgccctaa aatcaattta gctagttctt tgtcactttt tcgaatttct
     5521 tcgataactt gacttaacga atgcttacct ggcatggtgt aaactacatc gatggcgtct
     5581 tggatcaccg ttttcttgtg gctcgctgtt tctcgattgg tatctacttc tcctaacatt
     5641 cctaacacat ttttagcggt ttgaattgcc tcttcttttg gtaaaaacaa tagaggatca
     5701 agcatccctc gacagctttc atctgaagat aacgaaataa aattaattcg ttcatacata
     5761 gccacaaact ctggtatgtg tccgaatttt tcaagtgcct ttaaaaagaa cttttgggtt
     5821 tcatttttcg gatcaatata aagaattttt tgacctaaaa acatcagcca taaaaaaata
     5881 tattttacaa ggaccgattt tcctttacct ggtggaccaa taatcaacgt atttccattg
     5941 gtatgttccg cgccttcaat acttttcttt gttaaattgg gataatacca tactagttta
     6001 ctagatagcg ctaacgcttc tttcgcagat ttaaactttt ttgcaataac aatccgtcct
     6061 aaaggaaatc ctttcttatt tcctacttct ttctttaagt ccaagccata atctgccaca
     6121 aaacctgtcg tagcgacctg ctcgtaacta cgaaaacgtt gcgtacttcc aattaagcat
     6181 tgattaaaca acgttagctg atccgctacc gatcgataaa atttgaaatc tgtagactca
     6241 atcgcaaatt caatatcttt gactcgttct tccaattctt cttttgaaga agcagataca
     6301 accagcgtga cggtagaata acacaatcgt ttttctttgc gcttcagctt atcttttaca
     6361 tccattaacc gttctgtacc aaaatttaat acatcgtctg aatctaaaat gccgtcaacg
     6421 gtctcatatt ctttttcttg ttcataaatc cgtccagcca ttttattaat ttttcgttca
     6481 tcagattttg aatgctcaaa tcgaagtcga atatgcgtat caattggaaa agataagcta
     6541 tcctgaagat tttgaataaa gctactccca tacatggcta aaggagcgtc cgtttgactt
     6601 aaaaacgtcg aataatgcgt ttgattcaac tgttcaatcg caatgtatcc tgcttcattc
     6661 gaaatgacgc cctcgcaaat ttccattggc gtaagctgtc tttcaggcaa tttttcttct
     6721 gatcgatgga aaaaataata ataaatttgt ccaatctctt gggcagataa gcgctctatc
     6781 ggcttataat tggacaattc cgtatagagc tccttttcta atgtttcata cgttggtaac
     6841 acactactta taggaactcg ttgtcccgta atacgataga taaacttttt aaaaacatgc
     6901 ttaagcaatt gaaaatattc gatcggatca gacactttca tttctgtagt gaaacgcaca
     6961 aacagatacg ttcgatactc gtttaattgc acttcgtctt ttaaaatctt tcctgcctgt
     7021 tcaaagtaca ctttcccaag atctgcgcat tctcccttta ccaaatctcg aatggtttct
     7081 tcaatgtgcg tatctaggtt aaattgacgt ggaatattga taaaatgata gtcatattca
     7141 tcatgtccaa gtatgccttc gccatcttga agatagttct tgaaaaaatc aatgtcattt
     7201 aaaggaaata cttgggaaga aagttgatag cctacccaca catcatcatt tttggtcaaa
     7261 acaaaatttc ggataatggc gtctatcgga aattctaatc gaggaagctt cgtttttttc
     7321 tttttgaact gccaaatccc gataataaag acgaccaaaa gaaacaaaaa cgccatttac
     7381 tcgactcact ccttccattt gtgtagggaa tacgtcactt tttcatggaa aaacggcaca
     7441 tacatttcat gttcaaagcg ttgtttccgt ttctgataat aggacgttcg cccaattaaa
     7501 tactcgaaaa agtgtttatt gtcccatttc attgaaaaca atgtccaaac gagaacacca
     7561 atcccaaaaa tcataatcaa ccaggacttt cgaaacagtg tctgcaagaa actaatttga
     7621 ttcacatacg caatcaaaaa taggatcaaa agaatgaaga gaaatacccc acctatgact
     7681 aattgttcca tgcgaatgcc attagaaatc cagggaattt tttgtccttt aatcccgtaa
     7741 attttgtgag ggtgcttaaa ctcctttgta tagttgtatt ccatgttcac tctacctcct
     7801 tttacaaaaa aagaagcgac cagaagttct aatcttctga tcgcttttgc agtttgtctt
     7861 tttcttatga taattgtatc cagggcacaa tcatttgcgt tacccccacg ttctcttcac
     7921 tatccttaat aaagataatg ttctttagcc attcaccaat atcaacggca ttcgataaca
     7981 ataagacgag cattatcaaa ataaagacgc caataatggc aaacgctgcc cgcgcaaagc
     8041 cttgggtcgc ataagcacca accattagaa caatcgcaac gattgccaat agccaaatga
     8101 cttgtccaga tacatcatca aataacgatt taagatcaaa aagtttcatt tccattctcc
     8161 tctaagttca ttttattcat ttaacgtagt aacaaaataa ctatcgccac ttgtttttac
     8221 ttccattgaa aagtttgatt ggaactcgtt tcctttctca aaatagaaac gatacttccc
     8281 ttgcaaaaag tagtttcctt ctttattttt tactgcatga tccagttgta cattgatcaa
     8341 ttcgccgcct tctaaccctt tttcgttaga aatcaatgcc aatttttcat catttttgac
     8401 atataactca aaaaaccgtt tcacaaatgc ttccagcttg atttcttcgc tttcagtgac
     8461 cgcttgccca gaagaataaa atcgattttc ctgatagttc acagaattgg tattcttact
     8521 ctcagaaaga ttcaaaaatt gaacgccatc aagtactttt aaaacattgt tttgataact
     8581 gactggaaaa gaaagttgac tgcttttcca atgttctcct tctgtaaaag taacctcata
     8641 aaataggcga tacattgctt gtgtcaaatc gatttcttct agtttaataa agcgaacatc
     8701 tttcgctact cgattggtag ccgtcgttac aaagcctaga tcagacgctt ccaatccttg
     8761 tgctaaataa tgattgacgg aagcctccca ttcgtttcgt ttttctggcg aataagtaaa
     8821 taacctttca ataaaatgtt gtccctcgta cttcaattgt tccgtagaag acaaggtttg
     8881 gttgacttca gttaacaatg tttctggatt gacttgtttt ttcactgcta aacgcgttaa
     8941 tgtatccact cgtccaaagt ttaggaggag cacgacaccc attaacagaa acaaacaaaa
     9001 aaaagcgcct gaaaatacac tcatggcact tttttgcgaa aattggatca ttcgatctga
     9061 cggttctgtt ttcttttttt ggaacaaaaa acgtttcttt tgtttctttt ccttctgttg
     9121 tttcggtggc ttagtttgga acgcttgtgg attaaatttt gcattactca tgagaaatgc
     9181 cataccgttc taacaataat ttcggtattt tgtccacaat cactacttcg tggacaacca
     9241 cacttgcccc ctcc
//
LOCUS       DANKAZ010000096         9027 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000096 DANKAZ010000000
VERSION     DANKAZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(244..1296)
                     /locus_tag="PWY67_002290"
     CDS             complement(244..1296)
                     /locus_tag="PWY67_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2058514.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1309..2247)
                     /locus_tag="PWY67_002291"
     CDS             complement(1309..2247)
                     /locus_tag="PWY67_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2058515.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2698..3348)
                     /locus_tag="PWY67_002292"
     CDS             complement(2698..3348)
                     /locus_tag="PWY67_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058516.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(3448..3957)
                     /locus_tag="PWY67_002293"
     CDS             complement(3448..3957)
                     /locus_tag="PWY67_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDL2058517.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(4158..5027)
                     /locus_tag="PWY67_002294"
     CDS             complement(4158..5027)
                     /locus_tag="PWY67_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2058518.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(5047..6168)
                     /locus_tag="PWY67_002295"
     CDS             complement(5047..6168)
                     /locus_tag="PWY67_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2058519.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(6170..6565)
                     /locus_tag="PWY67_002296"
     CDS             complement(6170..6565)
                     /locus_tag="PWY67_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2058520.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(6584..7390)
                     /locus_tag="PWY67_002297"
     CDS             complement(6584..7390)
                     /locus_tag="PWY67_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2058521.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(7383..8171)
                     /locus_tag="PWY67_002298"
     CDS             complement(7383..8171)
                     /locus_tag="PWY67_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2058522.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(8185..8661)
                     /locus_tag="PWY67_002299"
     CDS             complement(8185..8661)
                     /locus_tag="PWY67_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2058523.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(8733..>9027)
                     /locus_tag="PWY67_002300"
     CDS             complement(8733..>9027)
                     /locus_tag="PWY67_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2058524.1"
                     /translation="TKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLVKQ
                     NTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         2842 a         1940 c         1299 g         2946 t
ORIGIN      
        1 attattcgat acatccaaca atcaaatttt ttctcatttt aacacatatt tttattctag
       61 ttgtcttttg aacaatcaat aacataaagg tgagtttctt ctacatttta aaaaatcgaa
      121 tggcactgaa gagttcagtt gtcagcatca atctcctact caaaaaggca aaaaaacgta
      181 agtcagatgt tgttgacatc tcacttacgc atccattgat tgaaaatttc ttagtaaaat
      241 ctactagcct aaaaatttga agacttcttc tcgatcatac agtttaaata ctttctcgca
      301 taaaaattga gtaattagtg ccgcaaaaac agggatgact aaccaacaaa gaacaacaag
      361 aaagccattt agccctgcat ctaaagaagc caacggacca acaagtccga ccacgccgaa
      421 accagcagaa gcaggcgtac ctgaaataga aaatagagcg acaggaatag ctgtaatcac
      481 tgcactaact aaacaaggaa taagaatcac aggttttcta aatagattag gcatcatcat
      541 cttcatagcg cctaaagcaa tcgcaattgt cacacctgac ttattaacct tccaagagtt
      601 cactactaaa acaaaagtgg ttgcagctac gcccatcgca gccgcaccag cagaaacacc
      661 atctagttgg attgccattc cgattgctac tgttgaaata ggagagataa tcaaaaaggc
      721 aaaggaacaa ccaatcaaga tagacattaa cagcggttgt aaggtagtga agttattaat
      781 caatgatcca attgcagaag taattgctgt tacaaaaggg aataaataaa aaccaagtaa
      841 acctgcacct acacctacaa caatcggtgt taacacaatc gacactgacc caaaactatt
      901 tccaatcagt aaaatcagcc cgacagcaat cgatgctgtc agcatcgtat taataatatc
      961 gcctgtacca gcaccaacgt aaacacccgt ttcacccaaa tcaggtgtga atttgactac
     1021 accagaacca acaaaagaag ctccggctac aaccatcatt ctcatcggat caaatccaaa
     1081 ttgtaaggca atcaaaccac caatgatcaa aggtgttgct agttgaaaaa taactgctaa
     1141 ctgtgcgatc gttaccgcaa agggatacgc actaaaatat tttaaaatgg cacctaatac
     1201 tgcattagga atcaatccaa taatgattcc tgttgctgta ccagcgagga ctttattcag
     1261 aaaaattttc ggtgatattt tttgtgaagt ttccattcaa tctctccttt attttacgtt
     1321 caaactatct tcttttgcat ggacaagctc agtcaagaaa tcacaataag gcgtcgcaac
     1381 accatatttc ttcccttttc tagaaattgc gccattgata taatcgattt ctgttaatcg
     1441 atggttctta atcaagtctt gatacattga aggataatgt aacccaattg tttccgggtc
     1501 aaaacaagat tcacaatgtg caatgacttc tgggacatct agttcaatct tctctactgc
     1561 tgctactttg gcaaactcat tgacaatcgt cgccaccatt ttatgagcag tcgatgtttt
     1621 tcctagctca gccatattaa cgtctaagat tgtgcatagt ccgttcattg ttccgttcac
     1681 acatgctttg cgataaatag aatagtgaat attgtcagaa aaatgagcat ttaatcctga
     1741 ttcagacaat ttgtctgcta atttttttgc agctgcttct tttccatcac ctaaattttg
     1801 taattctact gaaccacttc caaataattt gacctgacca ggaccttcaa gacctgccgt
     1861 ccacatggta tttccaatat agatattttc cattggtaca aatttctcaa taatatcttc
     1921 atgcccgata ccatttaata gacataagac ctctgtatct tttttgatta atgattggat
     1981 atcctgcagc attttttcca gctgcatcgc tttggtaaat agaataatca gatcaacttg
     2041 atcttctttt tctacttcgg attgaagaac gattggtagt tttgcttcta cctctttacc
     2101 attaaaattt gcttgtaatc catgttcttt gatttgttgg acatgttctg cccagccatc
     2161 gattaataat acttcgttcc cactttggtg aagcatcaac ccaaagcgac ttcccattgc
     2221 gccagcgcct gcaattgcta ttttcattga ttcttcctcc tttttctttc tattatactc
     2281 atttgagaat cttttcacaa cctcgttttg ttattcttct cttatttatc atgtttatat
     2341 ccaccattca agttatttta tcagcaaatc aataaaaact ggaatagaat cccaatttca
     2401 tcgtaagaga aactagacta gcaataagaa aatagaaaca ttcatagaaa aaatctcact
     2461 cctctgatta taaaagaggg ggcattaacc tcgtgtatac taaaaccttc cattcattcg
     2521 atggatgatt ttctttttgt gaaaaaatat tcattgtagt acgatgttgc gcaacacaga
     2581 cggatggtat tgcttattat ttattaaaat cgaacaatac acttatttaa aaacttcttt
     2641 cccagatttt cttccatttt tacagttata actagtatta tcatgaaaaa tagcctttta
     2701 ttgtctatgg gaataataat ttaaaattgg tactttttta ttaattagtt catcgcccaa
     2761 taatctctcc agtttcttag catctcctga ctcaaccgga attatccaag cccagtctaa
     2821 ataatttttt aaaatctcat catattttgc aaatatttca ttgccatatt ccgtccttaa
     2881 aattatattt tcagtgccag acaagtgatt tatattgagt tgataaaaaa gatctccatc
     2941 taaaaaaata tcattagaag aagatttccc cacagtaatg accattccat cagctctatg
     3001 tgttacatac aaataattgt atgacctcat ttcacctaat gaatcaatta tttcttgccc
     3061 tttagctcta atatattgat ctctattacc tatctcactc ttatttaaca aaagtttttt
     3121 catgtcaaat ttaatcggct gttttatttt tgtaaattcg cttctactac taatccaatc
     3181 cgagattaat ttttcaacat gtcccagagt aaactctgat ttattcgcta aatacttttt
     3241 ttgttgtatt aaagacatct ttccccgaag ctcaaaattg agcctacatc gattctctat
     3301 ctcttcaaaa agcatcttat cttcatcatt cttatattca tcattcataa aaccaatcca
     3361 gctcctcgat taagataata ttttaatctc atacaaaaac tagctctcat caaaacaacc
     3421 attgttttga tttctcatga aatttcttta tacttttctc cgattataaa ttcctaaccc
     3481 cttgagaaaa ttacgcatat atttatctcc gcaaggctta aaatttcgtt ggccttcttt
     3541 tctcaaaaat gccccgattt cgccttttga tggatagatt tctgcctcag caaaaacgtc
     3601 taaaatatca tcagtggtca tagacaaggc gatttttaat ttttttagca acacattgtt
     3661 gatatatttg atttccttgc ttttttgcgg cgtgtctact atcggcttat ctttttgcgg
     3721 accacgcgcc aagagaatca ggccatttag gaatgcgtca aaaacttggt tgttgatggt
     3781 tttttcatac acattatttt ctgcattatc tttgttctta tcctgtactt tggtcagtac
     3841 cgcctgagct tcttctttag tcagtggtac accgcctagc tcaaaagctt taatcaaatc
     3901 cgtatctttc aaatcaatcg cataacgcaa tcgcaataac ctatcattgt tattcatttg
     3961 ttcctccgaa gtatttagtc tttactttct tacgtacttt ttgtcttgct aagattttta
     4021 ggatcaatga taaaaaccgc atcacttatg ataaggcgct gggtatcatg agtgaatgat
     4081 attgctgaat acaaatagtc cgaccacatt aagttgatcg gacttcataa gcattttatt
     4141 gactaatact tttttgctta agaattgatt ctaacatctt ttgctaaagt taagcatcct
     4201 atagttcctg cattatctgc taacttaggt gtgacaatgt attcttcgat atttggcgta
     4261 tcgacatagc cgttaattag ttctaaaaaa cgtctttgta ctttcttttt cagatgttct
     4321 tgttgcatga caccaccacc aaaaattata atatctggtg aaagcatcag ggtcgtatta
     4381 caggcacatt gtgcaatata atccgcctcg atttcccaga acggatcttc tatagaaaga
     4441 tcttgtccct tggttcccgt acgcatctcg atagcgggtc cagatgccaa cccttcgaga
     4501 caatcatgat gaaaaggaca agtaccagag aaagtatctc ctgaatgtct cttaactaac
     4561 gtatgcccca tttctggatg gctaaaccct tcaacaaaaa tcccatcttg tatagctcct
     4621 ccaccaattc cagttccaac agtgtaataa acaacactgg ataaaccttt tccatgacct
     4681 gaaacatact ccccgtaaca agcagcattc acatcggttg tccaggcaat tggaatttcg
     4741 aaatgcttat tcaacgttcc aacaaaatca aaatttttcc aggcagtttt tggagtagag
     4801 gtgatatgtc cataggtctt tgattcacga tggatatcaa tcggtccaaa tgaccctact
     4861 ccaatgcttt ctaattgttc tttatagtga ttaaagaaat caattaccaa actcattgtt
     4921 tcattcggtg tagttgtagg aaaactcact ctttctaaaa cctttaaatc atcattgcca
     4981 atggcacaaa caaatttagt cccacctgct tctattgagc catacatttc ttactccacc
     5041 gtccttttat ctaggaattc gttcttccct gtctaatagt ttttcgaaaa actcatgtgg
     5101 ttccttttgg taccaatagg caacagaaga aatatcgtct tgacgctcat aaagcttgat
     5161 gtcatcattt cctagggttt ggaaagtaac acgtaaattt tctttgaaac taatggggtc
     5221 gggtaaatgc cagcggtaca acccatgcat cggtaaagca tcatcgccaa aaccatgaac
     5281 tggatttgga ttaggttttc cagcactaaa ataatcccgg gtgtgatctc ttttagattg
     5341 aaaaggatag cccataaata gcgtattaaa attttgagca tatgcccttt ctccaaagtt
     5401 ctttttatgg aaagcccaag cgccgccaaa ataatcttct gctcctgtag atgttattgt
     5461 agggaaatct tcatctccat ctatataaaa cttaaattct ccttcccccc accagtatct
     5521 ttccaatgcg cacaaagcaa agtaggttcc tacataatac ccagtgcctt tgattccatc
     5581 aataagggtg taatcttttc cttttgtcgt gatgggttca cggttccatt tagcatgaaa
     5641 atacatcgca tcatccggta cttcagtcaa tgcataatta aatgtataaa aaatgtgctt
     5701 aataaactca ggatgttcat tcgttaaagt aatcttagct ttttttcgaa atggcatctc
     5761 aaaatagcaa ttcatccctg atgttggatt cactacgatt ggtaaggaat tcacttcgca
     5821 tcttgtccca aacccattac aaaaaaaatc tcctaatgga ctttctacag aaggagtgga
     5881 ttcatcatcc caataaatac gcaaaattaa atcacgtaag acgaaactcc cagcgattgt
     5941 tttgtctgtc actgtcatcc aaaaatgacg taaaattcca gggccttcca tttctgctaa
     6001 agtaattgtc tctccagcat ttatcggaac agaaggtgac ccttttctcc caattcctag
     6061 atgactcgct gtcattgccg ctttcccttt ttcaccattt ttattttctg gtgaaatagt
     6121 tctactttcc tctcccttgt ggagccatat attatttaaa caattcattt tattcgtcct
     6181 ctatcctttc ttcacttttt tgattattca tatagatgat ttgttgttgt gcctccgcaa
     6241 taattgctcc aatatctaaa ttatcatttc ctaactgcaa agcgattgac attgccaagg
     6301 gaagattcat tcctgcaatt acgtgcactc tgtcagaaat ataggtaaaa aacttttgat
     6361 ttacacttcc tccagccatg tcagttaaaa caattatttc gtctgtagta tccactaact
     6421 ctttaatttg ttcatctata tcatcaattt cttcgacata tgcacataag ctactaattt
     6481 gattatgcat acctgttaaa aactctagtg tctctttaaa accctcagct agtattgaat
     6541 ggctagcaat gatcaatctt ctcataattc aaatcctttt cctttacgct aatattccaa
     6601 atgcagccat aaccatcgca aaaacaataa cgagtagaat caaaatggtc atactgattt
     6661 ttttcttact gagtagataa tatgctgcag cagtaataat tactggaatt aatgttggta
     6721 aaattttgtc caacattggt tggattttca tactaacttc accaaaagtg aatttaagtg
     6781 gcgttgaaac actgataact gaagcaatta atgatccaac tactgttagc cccaatactg
     6841 aagcagcttc agttaaataa ttaatctttt caccgtattc agtaacaatc ttcattccct
     6901 gcttataacc aaattcaaag cctctcattc ggaaaaagaa gattaccatg attaaaatga
     6961 accataaaaa gattcctaca ggattatttt ctaaagccat ataagccgca attgatccca
     7021 tgatggttgg gatcatcgtc atcaaaagag agtccccaat accagcaatt ggacccatcg
     7081 taccaacttt aaagtcatta atcgcatctt ttgcttctaa tccttgcttt tcctccattg
     7141 caacacaagc acctaaaata atggctgcca gccaaggctg tgtattgtaa tactgaaagt
     7201 gattatttaa agcttcgatg tattcatcat catctttgta taattttctt aaaataggtg
     7261 acaatgcgta agtcacagat gcaccttgct gtgtttgata attaaaggta caaactgaca
     7321 tcaaccaacg atagttcgct ttcctaagat cctttttcgt tacttgatat ttttcgctca
     7381 ttttattcat cctccccaat aaacccaaca tcagaagctc ccgcagaagc agtgatttgt
     7441 gcatcttttt cagctctatg atacacatta aatgctaaag cgaatcctat gatcgccgct
     7501 ccaataatag gtaccttcaa aaaagcggct aagaaaaatc caacaagtaa ataactaatg
     7561 tatcgacctg taggcataaa gcgtaacaac atcgcaatac caacagcagg caacattttt
     7621 ccggctaatg ttagcccatc cataaaccaa ttaggtaaaa catccaaaac gtaattaaca
     7681 gcagactgtc ctaaagtaac ggcaataaat actggaattg ctgcagataa accaaagaaa
     7741 attggcccca accacaaaac actattcatt ttcttaaatt ctcgtttatt cgcatattcc
     7801 tgtgatttgc gaacaacaaa accatttaag attttgacaa ttacatctag ttgcacccct
     7861 aacataccta caggtagtcc tattgctaat ccggcttcta ctccaagctg gctaccaaat
     7921 gccgttgcaa taattgcgca cgttccatag tccggtacag atgagccacc aaaaccggca
     7981 actcctaatg acatcaattg aactgtaccg ccaactatca aacctgtttt tatatcaccc
     8041 atcaaaattc ctgtgaataa tccccaaaaa actgcgtaat ttacaaagat catcggaatc
     8101 ccataataat caacatgttt aataaatgct aatatagtca gcataataat ttgaaaaata
     8161 gtaaaatgca ttatccttac tcctttatcc taattttgat tgaatttcct gtgccttatc
     8221 cgccggaaca aattgtgtaa caatttctac accagaatcc aacaattttt tatacgtgct
     8281 gatattttca tctgatgcat aggctctaga agatagctgt aatgcattat ctttaacaac
     8341 agtaccgcca acaattagtc ggtcaattct aataccgtta tctactaaat gaaggattgt
     8401 ttccggctct ttcacgatga caaatacttt ttcctgatcg tatttcccct ttttaaaatt
     8461 cgcaatcgct gtttcttcag tgataattga tacagccatt ccagcaggtc tagctaaacg
     8521 catacttgat ttttggatgt cgttatttgc gacagaatca tcgatcacca tcactctgtt
     8581 tgctttagac attggtgccc attgcgtgac aataatccca tgcaataaac ggttatcaac
     8641 tctcgacata acaatactca taaatacttc ctcctttatc tttcattttc ttacaagcaa
     8701 ttaccatgcc aaaacaaaaa gaacttgata aatcaagctc tttttacata atcataaacg
     8761 tatttaacct catgaatcgg gatgtcgaca ctataataat gttcaacgac actgaaagat
     8821 tgcttaaaat gtgtctgaaa tttttgaaat tcctcactcg tttcattgaa ataaatttca
     8881 tcttccaaag tattttgttt tactaatcgt tcaattaagc aactaatgtg aacatataat
     8941 ccgaaacacg tattattatc aaatgaaatt cctagatcat tttgtaagat gtatatagca
     9001 tctgaaaccc gttctaataa tttagtc
//
LOCUS       DANKAZ010000097         8919 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000097 DANKAZ010000000
VERSION     DANKAZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="PWY67_002301"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="PWY67_002301"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL2058525.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1711..3057)
                     /gene="gor"
                     /locus_tag="PWY67_002302"
     CDS             complement(1711..3057)
                     /gene="gor"
                     /locus_tag="PWY67_002302"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL2058526.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3191..4444)
                     /locus_tag="PWY67_002303"
     CDS             complement(3191..4444)
                     /locus_tag="PWY67_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2058527.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4450..5373)
                     /locus_tag="PWY67_002304"
     CDS             complement(4450..5373)
                     /locus_tag="PWY67_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2058528.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5594..8086)
                     /locus_tag="PWY67_002305"
     CDS             complement(5594..8086)
                     /locus_tag="PWY67_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL2058529.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8093..8560)
                     /locus_tag="PWY67_002306"
     CDS             complement(8093..8560)
                     /locus_tag="PWY67_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL2058530.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8696..8769)
                     /locus_tag="PWY67_002307"
     tRNA            complement(8696..8769)
                     /locus_tag="PWY67_002307"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8733..8735),aa:Arg,seq:acg)
     gene            complement(8827..8900)
                     /locus_tag="PWY67_002308"
     tRNA            complement(8827..8900)
                     /locus_tag="PWY67_002308"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8864..8866),aa:Pro,seq:tgg)
BASE COUNT         2478 a         1884 c         1485 g         3072 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
     1081 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1141 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
     1201 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
     1261 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
     1321 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
     1381 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
     1441 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
     1501 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
     1561 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
     1621 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
     1681 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
     1741 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
     1801 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
     1861 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
     1921 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
     1981 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
     2041 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
     2101 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     2161 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     2221 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     2281 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     2341 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     2401 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     2461 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     2521 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     2581 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     2641 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     2701 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     2761 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     2821 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     2881 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     2941 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     3001 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     3061 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     3121 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     3181 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     3241 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     3301 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     3361 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     3421 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     3481 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     3541 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     3601 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     3661 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     3721 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     3781 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     3841 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     3901 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     3961 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     4021 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     4081 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     4141 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     4201 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     4261 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     4321 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     4381 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     4441 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     4501 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     4561 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     4621 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     4681 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     4741 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     4801 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     4861 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     4921 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     4981 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     5041 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     5101 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     5161 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     5221 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     5281 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     5341 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     5401 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     5461 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     5521 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     5581 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     5641 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     5701 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     5761 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     5821 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     5881 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     5941 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     6001 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     6061 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     6121 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     6181 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     6241 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     6301 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     6361 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     6421 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     6481 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     6541 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     6601 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     6661 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     6721 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     6781 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     6841 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     6901 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     6961 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     7021 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     7081 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     7141 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     7201 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     7261 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     7321 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     7381 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     7441 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     7501 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     7561 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     7621 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     7681 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     7741 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     7801 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     7861 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     7921 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     7981 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     8041 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     8101 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     8161 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     8221 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     8281 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     8341 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     8401 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     8461 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     8521 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     8581 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     8641 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atcgacgcgc
     8701 ccaagaggag tcgaacccct aaccttttga tccgtagtca aacactctat ccagttgagc
     8761 tatgggcgca tattcaattt aaaaaaatta taaatcattt gaaaacaaaa agcaagataa
     8821 aaaaaatcgg gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta
     8881 ccaagctgag ctacttcccg tttaaaaaat aaaaaagac
//
LOCUS       DANKAZ010000098         8904 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000098 DANKAZ010000000
VERSION     DANKAZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(305..661)
                     /locus_tag="PWY67_002309"
     CDS             complement(305..661)
                     /locus_tag="PWY67_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL2058531.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /gene="spx"
                     /locus_tag="PWY67_002310"
     CDS             complement(675..1085)
                     /gene="spx"
                     /locus_tag="PWY67_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2058532.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1432..3618)
                     /locus_tag="PWY67_002311"
     CDS             complement(1432..3618)
                     /locus_tag="PWY67_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL2058533.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3836..4519
                     /locus_tag="PWY67_002312"
     CDS             3836..4519
                     /locus_tag="PWY67_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL2058534.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4600..5319)
                     /locus_tag="PWY67_002313"
     CDS             complement(4600..5319)
                     /locus_tag="PWY67_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2058535.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5631..5837)
                     /locus_tag="PWY67_002314"
     CDS             complement(5631..5837)
                     /locus_tag="PWY67_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058536.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6082..6684
                     /locus_tag="PWY67_002315"
     CDS             6082..6684
                     /locus_tag="PWY67_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2058537.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6825..8105
                     /locus_tag="PWY67_002316"
     CDS             6825..8105
                     /locus_tag="PWY67_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2058538.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8118..8846
                     /locus_tag="PWY67_002317"
     CDS             8118..8846
                     /locus_tag="PWY67_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2058539.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2587 a         1667 c         1463 g         3187 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaat
     1201 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1261 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1321 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1381 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1441 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1501 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1561 tagtcttcgt tcttcttcta tcgtcgtcac tggttctgct tttatttcat taaagtcgat
     1621 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1681 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     1741 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     1801 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     1861 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     1921 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     1981 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2041 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2101 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2161 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2221 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2281 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2341 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2401 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2461 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2521 ataattagtc aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2581 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2641 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     2701 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     2761 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     2821 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     2881 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     2941 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3001 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3061 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3121 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3181 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3241 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3301 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3361 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3421 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3481 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3541 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3601 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3661 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     3721 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     3781 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     3841 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     3901 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     3961 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4021 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4081 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4141 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4201 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4261 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4321 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4381 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4441 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4501 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4561 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttatttg taaagattcc
     4621 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4681 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     4741 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     4801 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     4861 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     4921 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     4981 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5041 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5101 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5161 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5221 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5281 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5341 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5401 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5461 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5521 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5581 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5641 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     5701 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     5761 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     5821 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     5881 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     5941 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6001 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6061 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6121 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgccttagaa
     6181 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6241 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6301 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6361 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6421 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6481 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6541 tctctcatta ctttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6601 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6661 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     6721 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     6781 taattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     6841 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     6901 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     6961 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7021 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7081 ttttaggaga tcacttaaca agcatcgacg atttttttgt tttgaaatct tcggttattg
     7141 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7201 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7261 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7321 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7381 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7441 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7501 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7561 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7621 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7681 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     7741 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     7801 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     7861 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     7921 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     7981 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8041 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8101 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8161 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8221 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8281 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8341 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8401 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8461 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8521 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8581 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8641 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     8701 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     8761 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     8821 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     8881 agccgatcca aacccttcgg ttct
//
LOCUS       DANKAZ010000099         8835 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000099 DANKAZ010000000
VERSION     DANKAZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..832)
                     /locus_tag="PWY67_002318"
     CDS             complement(<1..832)
                     /locus_tag="PWY67_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HDL2058540.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     D"
     gene            complement(838..1944)
                     /locus_tag="PWY67_002319"
     CDS             complement(838..1944)
                     /locus_tag="PWY67_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL2058541.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(1944..3080)
                     /locus_tag="PWY67_002320"
     CDS             complement(1944..3080)
                     /locus_tag="PWY67_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2058542.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(3112..4563)
                     /locus_tag="PWY67_002321"
     CDS             complement(3112..4563)
                     /locus_tag="PWY67_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2058543.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(4607..5371)
                     /locus_tag="PWY67_002322"
     CDS             complement(4607..5371)
                     /locus_tag="PWY67_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL2058544.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(5399..6097)
                     /locus_tag="PWY67_002323"
     CDS             complement(5399..6097)
                     /locus_tag="PWY67_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL2058545.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(6109..6888)
                     /locus_tag="PWY67_002324"
     CDS             complement(6109..6888)
                     /locus_tag="PWY67_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL2058546.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6904..7848)
                     /locus_tag="PWY67_002325"
     CDS             complement(6904..7848)
                     /locus_tag="PWY67_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2058547.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7894..8673)
                     /locus_tag="PWY67_002326"
     CDS             complement(7894..8673)
                     /locus_tag="PWY67_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058548.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2505 a         1847 c         1310 g         3173 t
ORIGIN      
        1 tatcaaatgt ctgtgctatt tcatgattta ttctctgtgc taaaacaatc atataatcta
       61 tcatcaaata atccaccatc atatcattat ttttccaata atttaaaaaa aagtctctaa
      121 ttatagcaaa catccatcta gactcttgat tacatgctaa tgaataaccc gcaaaatatc
      181 ctgatgctac ggatgcgtga gcataatcag gcctcctaat agaccaaata tcgatatcaa
      241 aatactcatc tggtattggt tttgtacaat aaaatgtggc gtctaaccac aaacctccat
      301 gttttgctag caaagataat cttaataaat cagaataatt tgttctcgta atgattcctt
      361 tgtttttctt ttcttccacc cattttggta tatcaacata ttgtctataa ttatcttctg
      421 ttaaaattat aacagtatgt ttccctgcat ttcgttttat tgattttaca caagctttta
      481 caatttccgg cgattgatcc aatccctgcc accaacaaac ccaaatgcag ttagaatatg
      541 gtgattttga ttgattaatt atttgatttt gactataatc atatgttttc aaaaaatctg
      601 aaaaagtctt ttcataatac ttcagcatta cctcatgttt ttttaataaa tgttccttct
      661 gttttttact ttcattatat ccattacgat tcatgacttg aatatcaatt ttagcttgta
      721 acgtggcaaa tgcttccttc caagatgtta tctttgcaat tcgtcttgtt gcattcaatt
      781 cttcggatat gcgatgtata attcttttta tacttgaatg tttctgtgtc atattcttta
      841 acctctttgt attaattcaa ataactcata atattttgat tctaagattt tgtatgctgt
      901 ttgaatatta taatctgcct ttattaaaat cgaacgttct gaatctcgat gagagatatc
      961 tacatttgca atctctctcg cccattcttt tgccgattga tttaaactta tgaaattggt
     1021 cttatcactg aaagcaactt ctgtaggaac tttctccgaa aagaaacacg gtaaatctgc
     1081 aaattgagct tcaataccca ctaacggaac cccttcaaat aatgatggca acattaatac
     1141 gtcaaatgct tgataaaatt tttctatatc atttctattt cctaaaaatt taatatcatc
     1201 atctatgcca cttttctttg cataattttt tatttcttct tccatatcac cagttccaat
     1261 aattaataat acggaattat tcttaatagc gtgaaattct ttaaaaattt caatcaaaaa
     1321 acgatgattt ttagaatatg aaattcttcc aatatgtccc attacaaacg tctgacttga
     1381 aattcctaat ttcattcgta catcatttct aatactttca tcataataaa attttttagc
     1441 atccactgca tttggaatca aagtgaattt atcatttcga aacaaaaaat cacctgctac
     1501 ttttccacat gaaaattcat gtgtcaaaac agacgttaac ttacatcgat aatattgctt
     1561 taaaagatat ttaaaatctc tatctatcga tgtattatgg ctatgtgcta tccgtatcgg
     1621 aatcccagct ttttttgcag cttttaaagg taaataactc atcgcatcaa tatgagaatg
     1681 aacaatctca tattctggat gatccttaaa aaattgttcc atccatttaa aatatttagg
     1741 ataattttgt ggataaagtc tcggtgcata atagaccttt cctccaagac ttattatttc
     1801 ctcatcataa tcacttttat atggacgatg tgtcaaaaaa tcaaactgaa ttttagttcg
     1861 atctatattc ctataatagt tcattagcat cgtctccaac cctgcacgat gcatatcatt
     1921 cacacactgt aatattcgaa tcattatata tttcccctat acaaatttaa tgtttcttgt
     1981 acgattttat cccaattata tttactacaa ataaactcag tagcttgatt cttcaaaact
     2041 ttaaccattt ccgattgatt gcatgcctct tctaatctta tctttaaatc acttacatcc
     2101 gatttcttaa aaatcatagc cttgtcttct actacttccg tacactctga gatatttgaa
     2161 actatacaac agttcccata actcattgct tctaacaaac ttaatggcat tccttctaaa
     2221 tcacttggaa gtgtatagat atacgcatta ctatataatt cttctaattc ctgtccttgt
     2281 acaaatccag taaatataat tcgctcatca ccttttgcta attcctttag ttcatttgca
     2341 aattcatctg tatcagagct tcctccggct attataagtt ttttatctgt ctgaacatct
     2401 ttaaatgcct caattagata ccgaattcct ttttcgggaa ccaatcgccc caaaaacaaa
     2461 atataagaat cttttgttaa tccatacttt tctgtaatca atcctgctga ttttatttca
     2521 atttttttaa cgccatttgg tataaacctt gtttttcttc catatatatc ttcaaaataa
     2581 ttttggacgc tttcactaag tacaattatt tcatctgcaa attttacagc acacttttcc
     2641 cctaacatga tgtatgtact agcaagcttg ttccattttt ctctttgatg atctagacca
     2701 tgaattgttg caacacatct tttcccaaaa agttttggca accacaacat agcacatggt
     2761 ccttctgcat gaaaatgaac aacatcatat tttccgaatg ctgctctaat tccaccaaat
     2821 actgaagaac tcattgctgc aattcctttt atatttaatg taaaaatagt ctttaatttt
     2881 acacctttat attctttatt tttatgctgg tcaaattcct ttccgctaac atgatgacca
     2941 cttcgattat agcaagtcac ttcatgccca agtgcaacca ttcgcgttgc caattcttct
     3001 accactatct caattccacc ttctcttgat ggaattcttt tatgacctag catacagatt
     3061 tttaattttt ttcttgccat cacgttttac tccttctata gtatctttat cttacatgga
     3121 accgtttcta tgaagcacag ttttgactgt cctaaacaaa attctaaaat ctaatcccat
     3181 actccaattc ctgatatatt ctgcatcaag ttttacaacc tcttcgaagt ctgtgatatc
     3241 acttctacca ctcacctgcc acattccagt aatcccaggt ttcatcgcaa ttctcgcacg
     3301 atgatgaaaa tcatacttct catattctga cttcaagcaa ggtctaaacc ccaccaggct
     3361 catttttccc attaggacat tataaaattg tggaaactca tccaatgatg tagatcgaat
     3421 aaattgtcca attcctgtct tctttgttcc atcaggaagt attttattgc caatgattct
     3481 tgggtcaaaa tccatcttaa acatgagatt attactcatc ttattttctt tcatcaactc
     3541 tgctttgcgt tcttctgcat ccatatacat actacggaac ttatacatct taaatgtctt
     3601 accattttga ccaatacgtt cttgtgcaaa gaaaatcggc cctggtgatt tgatgtaaat
     3661 aactggtgct acgaagataa aaataacacc tgttgcaata catccaataa ttccagcaag
     3721 aatgtcaatc gttctcttta taaatgcttg cctcatcgtc ataacattaa tactagatgt
     3781 aataacagta taatcaccaa ctcggccaat aaattgtttt ccacgtgtac tatcagacac
     3841 ttttgtaaga ttcatatgta cagcaagtcc catttccgaa catctggcga ttaattcttt
     3901 gggatatgga acactttcgt caacattgat gaatacttca tctacccaat tctgtaaaag
     3961 atattccgct gcattatcag catttgcaac tactgggaca cctccgatgg tagttccata
     4021 catatctctg tctactacaa tcacaccact aagtttatac atgccataat tcttctcttt
     4081 aagattacga actacatttt ttgcaatctt ttgagatgtt acaataacta acgatacatc
     4141 ttcaccgttt ttcatgcggt gatgcaacaa ccctttccaa agtatacgaa ttatatatgt
     4201 taataatcca tacaccaccc ccattataaa taaagaaact ctggaataat catttccatc
     4261 ctgcattgta ataaggtata aactgcacac aagtaacagc ataaaagatt gctttataga
     4321 agctacagcc tctttataat atcctctttt taaaacattt ttatatgcct caaataaaaa
     4381 tataatcgta agattctcaa gttctataaa aattgccatg tggcgataaa taggaatcac
     4441 atacggattc cagtcaccct gtcgcattac atatgatata taaaaagcaa gctggacaca
     4501 aaataagtct aataaaataa aatcaatatg tttagaccaa ccacttgagt tttttctata
     4561 catctctcac tctctcctca tttccttttt atctacgaat aaagaactaa aataaaccaa
     4621 acttcttctt ttttacctct tcatagattg gataattgat ctcatccccg ttgatcagat
     4681 cctttgcgac ttgtttcatc tgtactactt tttccttacc aaactccttg gcaatctgct
     4741 catagcactc ttttaagtaa aaggtgcgct tagagacccc gtgtgcatca gaagcagcca
     4801 tctgcacgag attatgtttg accattgttt tggctgtttt ctggatatct ttgccaaata
     4861 caccaacata acttggcgca gtaagttggg ccaaacatcc catatctagg aacggaagca
     4921 aatgatttgg gtcttctcga aaatgcgcgt tgcgttctgg atggacgatc actggtatct
     4981 ttcctagttc taacaactca aaaaaaagcc gttctgcata taacggcact tccagtgtcg
     5041 gaaattcgat cagaagatat gtatcatcta aatctgtaaa taaaatttca tctcggtgga
     5101 tgtcttcgat gagcgttccg gtaatacgta cttcttgtcc ttccaataac gtcaaaggca
     5161 actgtctttt ctctaattcg gcttgtaacg aagcgactag agaaatgact tgtgattttt
     5221 cattgctgta ttttccatta ttgtggtgtg gtgtgcataa aatatgcgta atgccttgct
     5281 gaatcgcttt ttcagccatt gcaatacttg cttctaagtt ttctgccccg tcatcgattc
     5341 ctggtaggat atggcaatgt agatcgatca tttctcttgt ctcctctctt cttattcctt
     5401 aatttccgta gtaataacca gcatctttgc tgtgttctgc tccgttgtat acgacaccta
     5461 agactcgtgc ttgtaccata tctaacagtt cttttgcttt agtcaatgat tcttttcgtg
     5521 acgtattttc ccgtacgacc aataaggtgc catccgtttt tgaagacatg atctgtgcat
     5581 ctgttacggc aacgactggc ggcatgtcga agatcactac atcaaatagt tgtcttgctt
     5641 ctgctaaaat ctgatccatt cttggcgaac ttagtagttc tgatggattt ggtggtttag
     5701 gtccacttgg cagaatcgac aaattgtcga ctggtgttcg ttggatctca tctgctacac
     5761 ttcctgatga actaagaaca gtacttaatc cgcttgcgtt gttcaacttg aacgttttat
     5821 agatgacggg cttacgtaag tctgcatcga ctagcagcac ccgttgacca gatttggcaa
     5881 aaacaacggc aatgtttgct gcagtggtcg atttcccttc tccaggtcct gatgaagtca
     5941 caacaatcgt tttgatttgc tgaccagctg cggaggcaaa ctggatattc gtccgaatcg
     6001 tacgatattg ttcagcgatt ggtgaagagg gattggtgag tgtgaccaaa ctgactgcat
     6061 gagtttgggc tgctttttgt ttctgtgttc gtgccatttt ctttcctcct agactcttga
     6121 gcggcttctg cgtgatggcg ctgttttatc gtttgtagct tttggttgtg tctgagctgg
     6181 tcctttttta cggataccag cattcaattc tttcgctgac atttctggaa ctgttcctaa
     6241 gattgggaat cctaatgtat ctgtcacata tttttcatct ttcactgtcc gatcgagtaa
     6301 ttccaataag aatgccagac ctacacctac catcattcct aacattaagc cagtcattaa
     6361 attcaattta ttatttggcg aaacaggtgt cgtattggca actgcacctg aaatgatcga
     6421 gattttatca acattcatca catcttttgc gttctcttgg aaaacttgtg aagttgtgtt
     6481 agcgatctgt tgagcagcca ctgcattcgt acttattgcc tgaatcgaga acatttgtga
     6541 attttctgcc tgattaactg agatggcatc tttgatttgt tcagcagtca ttttcacatt
     6601 gtcttctgtt tctaaacgat ctttgacttc attgagcact aaatcactga cgatcatatc
     6661 tttatacgta ttgatcattt gcaggttcgt attgacatca ttgacatttg cagtgtctga
     6721 ttgtggcagt gtcacgatca attgtgcttg tgaactgtat tttggtgtca agatgaaaaa
     6781 tgttacgatt cctgagatgg ccaagccagc aaacattgag atgatgatta ttaccaagtg
     6841 tttgcgtaat gtttcaaaaa tttctttcaa gctgattgtc tcttccattt tttctccact
     6901 ttcctattta ttctctagat tcatttgatc ttttaattct tttttcacac gatcaagttc
     6961 ttgatcactg actttttgat aggagatatt atcttgcatg aagccttctc cttgcagctg
     7021 atcttgctta atcgtaccaa atgcctcccg ataatctaaa gcaatttttt tcatttcatc
     7081 aaagttcatg tctgttttca tattcgactc tagagcattc aaaacggatt gatagttgct
     7141 gacaccgttt agactcaata cttttttcgc tacgccttct acgatctttc tttggcgccc
     7201 ttgtctaccg taatcaccat ttgggtcttc ataacgcata cgagaataag ataatgcctg
     7261 atccccgttc aatgtaattt taccgattgg aaagtcatag ccatcttgtg agaaagtgag
     7321 gtcattattg acttcgatac caccaactgc atcaacgagt tcctttagcc ctttcatatt
     7381 gatagagaca tagtgatcga ttgggatgtc tacatatttt tgtacggtat ccatcgccat
     7441 ggcagctccg ccgaatgcgt aagcgtggtt gatcttgtct tttgtgtcac gacctacgat
     7501 gtcgacatac gtatcacgag gaatgctgac gatcgttgtc tgtttgtctt caggactgac
     7561 tgtggcaacc atcatcgtat ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc
     7621 aatccccatc aatagaacag agaaagattc ttgtttgtcc agatcaactg gtttcgttcg
     7681 ttctccttgg gttcgttcca ctgattcgta agttttctca atagaatgtg agagatccag
     7741 atataatttt gctcctactc ctacaattgc taatactaat acgataagaa ctgctaaaat
     7801 aatcagaaat atttttttcc ctcttgattt tcttcttcgt cttgccatag tttcctccgt
     7861 ttgctaacct tgcttgaatc aacgttttaa ctgtcaatag cttatattgc tatagcccat
     7921 ttgctcagca acagtttgtg cttttgttgc gtatgctgcg cttatttttt cattccggat
     7981 ctgctctatt tgactgagca ccgtcaaata ttgatcgtaa gtagcggtgg cagtcacttg
     8041 accatccttt aacatcgtat cgaataactg atctagttgc gtgacttgcg cgatctgaat
     8101 atcagcgaat cctagatatt gcataacaac cgttttccaa ggactatctt taaaaaagct
     8161 catattttca cgtacacgag cgacatctgc acttgtcacc ttttctttga tgatgacatc
     8221 gtctacagct tgctgccagt ttgatacatt tttagtaaat aaatcgttga cagctgtctg
     8281 gatcttcagt tttgattctg cactatctaa ctgtgtcacg attgctttct tctcatcttg
     8341 gattgcaaca gcttctttag gcaattctga ttctttgatg tcgtaatcat ctgctgaaag
     8401 tttgacagag gatagcttat ttgacaaact tgtgattgtt tcttctgtta tttcagggtt
     8461 tagaaaaatt ttttcttttg tactatacag cttgtctact tcttttgata aagaagtcaa
     8521 ggtgtctttt tgattggtga tggcagattc tgcataattg atccgttgct gatgttgccg
     8581 ctgattattg atatggtatc caaacaaaca gatcgcaata gcacctgcac ctaccgccac
     8641 agcagtcaat gacttgacga atggcttttc catagattca ctcctaagta ttgatgatag
     8701 ataaattaag atataattca cttacataaa aatgacagct ttttatttaa aaaaacataa
     8761 ttaaactaat atattcacag aaatatttgt aaacaagttt tccctatata atcataccac
     8821 aaactgtttt cttga
//
LOCUS       DANKAZ010000100         8494 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000100 DANKAZ010000000
VERSION     DANKAZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..173)
                     /locus_tag="PWY67_002327"
     CDS             complement(<1..173)
                     /locus_tag="PWY67_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2058549.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="PWY67_002328"
     CDS             complement(593..1714)
                     /locus_tag="PWY67_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDL2058550.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="PWY67_002329"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="PWY67_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDL2058551.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="PWY67_002330"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="PWY67_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDL2058552.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="PWY67_002331"
     CDS             complement(4304..5215)
                     /locus_tag="PWY67_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2058553.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="PWY67_002332"
     CDS             complement(5232..6239)
                     /locus_tag="PWY67_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2058554.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="PWY67_002333"
     CDS             complement(6236..8365)
                     /locus_tag="PWY67_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2058555.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="PWY67_002334"
     CDS             complement(8370..>8494)
                     /locus_tag="PWY67_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2058556.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DANKAZ010000101         8464 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000101 DANKAZ010000000
VERSION     DANKAZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            474..725
                     /locus_tag="PWY67_002335"
     CDS             474..725
                     /locus_tag="PWY67_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058557.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="PWY67_002336"
     CDS             759..1037
                     /locus_tag="PWY67_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058558.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="PWY67_002337"
     CDS             1634..1891
                     /locus_tag="PWY67_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058559.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="PWY67_002338"
     CDS             2004..2615
                     /locus_tag="PWY67_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058560.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="PWY67_002339"
     CDS             2794..3351
                     /locus_tag="PWY67_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058561.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="PWY67_002340"
     CDS             complement(4050..4571)
                     /locus_tag="PWY67_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2058562.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="PWY67_002341"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="PWY67_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL2058563.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
BASE COUNT         2844 a         1371 c         1806 g         2443 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aaca
//
LOCUS       DANKAZ010000102         8203 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000102 DANKAZ010000000
VERSION     DANKAZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            165..590
                     /gene="lacA"
                     /locus_tag="PWY67_002342"
     CDS             165..590
                     /gene="lacA"
                     /locus_tag="PWY67_002342"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDL2058564.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            607..1122
                     /gene="lacB"
                     /locus_tag="PWY67_002343"
     CDS             607..1122
                     /gene="lacB"
                     /locus_tag="PWY67_002343"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDL2058565.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            1133..2065
                     /gene="lacC"
                     /locus_tag="PWY67_002344"
     CDS             1133..2065
                     /gene="lacC"
                     /locus_tag="PWY67_002344"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDL2058566.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            2068..3048
                     /gene="lacD"
                     /locus_tag="PWY67_002345"
     CDS             2068..3048
                     /gene="lacD"
                     /locus_tag="PWY67_002345"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2058567.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            3076..3393
                     /locus_tag="PWY67_002346"
     CDS             3076..3393
                     /locus_tag="PWY67_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2058568.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            3393..5099
                     /locus_tag="PWY67_002347"
     CDS             3393..5099
                     /locus_tag="PWY67_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2058569.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            5241..6647
                     /gene="lacG"
                     /locus_tag="PWY67_002348"
     CDS             5241..6647
                     /gene="lacG"
                     /locus_tag="PWY67_002348"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL2058570.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            6670..6942
                     /locus_tag="PWY67_002349"
     CDS             6670..6942
                     /locus_tag="PWY67_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HDL2058571.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            6963..7862
                     /locus_tag="PWY67_002350"
     CDS             6963..7862
                     /locus_tag="PWY67_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL2058572.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         2590 a         1407 c         1695 g         2511 t
ORIGIN      
        1 aacaaataaa aaaaacaaaa aataaacaaa aaaagtgttg ctttttgttt gaatgcttga
       61 tattatataa acaaagaaat gatgaaaacg ttttttcgaa cattttgcaa aatgttttct
      121 acttctacgt agcatttctt tctaaaattt aggaggtggt caaaatggct attgttgttg
      181 gtgcagatct caaaggtacg agattaaaag atgttgtgaa aaattttctt gtagaagaag
      241 gttttgaggt aattgatgtg actaaggacg gacaagattt tgttgatgtt accttggcag
      301 ttgcctctga agtaaataaa gatgagcaaa acttaggtat tgtaattgat gcttatggag
      361 ctggtccatt catggtagca actaaaatta aaggtatggt tgcagcagaa gtttcagatg
      421 aacgctcagc ttatatgaca cgtggacata acaattcacg aatgattacg gttggcgctg
      481 aaatcgtcgg tgatgagctt gctaaaaaca tcgctaaagg tttcgtcaat ggtaaatatg
      541 atggcggacg tcatcaaatc cgagtagata tgcttaacaa aatgtgttaa gaaaaggagt
      601 taaggaatga gaatcgcaat tggatgtgac cacattgtca cagatgaaaa aatggcagta
      661 tcagaattct tgaaatcaaa aggacatgag gtccttgatt tcgggacata tgaccacgca
      721 cgtactcact accctatcta tggtaaaaaa gtcggtgaag cagtagtaag tggtcaagca
      781 gacttgggag tatgtatctg tggtacaggt gtcggcatta acaatgcagt caataaagtt
      841 ccaggtgttc gctcagcgtt ggttcgtgat atgacatcag ctatatatgc aaaagaagag
      901 ttaaatgcga atgttatcgg tttcggtgga aagattactg gtggtcttct tatgaatgac
      961 atcattgaag ctttcattga agctgagtac aaaccaacag aagaaaataa aaaattgatt
     1021 gcaaaaatcg aacacgttga aacacataat gcacatcaag cagatgaaga tttctttaca
     1081 gaattccttg aaagatggga ccgtggtgac taccacgatt aaggtataac aaatgattct
     1141 gacagtcaca ctaaatcctt cagtagacat ctcttatcct ctagaaacat tgaaaattga
     1201 tacagtgaat agggtgaaag atgttagtaa gacagctggt ggaaaaggct taaatgttac
     1261 tagagtcttg tatgaatctg gtgataaagt tactgctaca ggtttcttag gtggtaaaat
     1321 tggtgaattt attgaaagcg aattagaaca atctcctgtt agtccagctt tttataaaat
     1381 ctcaggaaac actagaaact gtattgctat cttacacgag ggtaaccaaa cagaaatttt
     1441 ggaacaagga ccaacaattt ctcatgaaga agcagaaggc tttcttgacc actatagcaa
     1501 tcttattaaa caatcagaag ttgtgacaat ttcaggaagt ttaccttcag gacttcccaa
     1561 cgattattat gaaaaactta tccaacttgc ttcggatgaa ggagtagcag tcgttttgga
     1621 ttgttcaggt gcacctcttg aaacagttct aaaatcgtcg gctaaaccta ctgccatcaa
     1681 acctaataat gaggaacttt cacagttgct tggaaaagaa gtaacaaaag atattgaaga
     1741 actcaaagac gttcttaaag agtcactttt tagtggtatc gaatggatag tggtctcatt
     1801 aggtcgaaac ggtgcctttg caaaacatgg tgatgttttc tataaggtag atattcctga
     1861 tatcccagtt gttaatccag tcggatcagg ggactcaacg gtagctggta ttgcatcagc
     1921 tttaaatagt aaaaaaagtg acgctgactt attaaaacat gcgatgacat tgggtatgtt
     1981 aaatgctcaa gaaacaatga cagggcatgt gaatatgact aattacgaaa cactaaactc
     2041 acaaattgga gtaaaagagg tataaaaatg gtacttacag aacagaaacg caaatctttg
     2101 gaaaaaattt cagataaaaa cggtgttatc tcagctttgg catttgacca acgtggtgct
     2161 ttgaaacgtt tgatggcaca gtatcaagat acagaaccaa ctgtagctca aatggaagaa
     2221 ctaaaggttt tggttgctga tgaacttaca aaatacgctt cttcaatgtt gcttgaccct
     2281 gaatatggac ttccagcaac aaaagcattg gataaagaag caggtcttct tcttgcctat
     2341 gaaaaaacag gttatgatac atcaagtaca aaacgtttgc ctgactgctt ggatgtttgg
     2401 tctgcaaaac gtatcaaaga gcaaggcgct gatgccgtta agttcttgct ttactatgat
     2461 gtagacagct cagatgaact taaccaacaa aaacaagctt atattgaacg tgttggttct
     2521 gaatgtgtcg ctgaagatat tcctttcttc ttggaaatcc ttgcttatga tgaagaaatc
     2581 tcagatgcag gttctgttga atatgcaaaa gtgaaacctc gcaaagtcat tgaagcgatg
     2641 aaagttttct ctgatccacg ctttaacatt gatgttctta aagtagaagt accagttaac
     2701 gttaaatatg tcgaaggttt tgctgatggt gaagtagttt acagtaaagc tgaagctgct
     2761 gacttcttta aagcacaaga agaagcgact aaccttccat acatctacct aagtgcaggt
     2821 gtatctgcta aattgttcca agaaacattg caatttgctc acgactcagg tgccaagttt
     2881 aatggtgtgc tttgtggacg tgctacttgg gcaggatctg tagaacctta catcaaagaa
     2941 ggtgaaaaag cagcacgtga atggttgcgt actactggat tcgaaaatat tgatgaactt
     3001 aacaaagttc ttgttaaaac agctagtcca tggactgata aagtatagta aaaacataaa
     3061 acggaggata ttgttatgaa cagagaagag atgactctct tagggtttga aattgttgct
     3121 tatgctggag atgctcgctc taaactttta gaagcgctta aagcggctga aaatggtgat
     3181 ttcgctaagg cagatagtct tgtagtagaa gcaggaagct gtattgcaga ggctcacaat
     3241 tctcagacag ctatgttggc tcgagaagct tctggggagg aacttccata cagcgttacc
     3301 atgatgcatg gtcaggacca cttaatgact actgtcttat taaaagatgt gattcatcac
     3361 ctcatcgaac tttataaaag aggagcaaaa taatgcataa actcattgaa cttattgaga
     3421 aagggaaacc gttctttgag aaaatctctc gaaacatcta tcttcgtgct attcgtgatg
     3481 gatttatcgc tggtatgcca gtcatccttt tctcatctat ctttatcctt attgcctatg
     3541 tcccaaatgc ttggggattc cactggtcaa aagacattga gaccttcttg atgactcctt
     3601 atagctattc gatgggtatc ctagcattct ttgttggtgg tactaccgct aaagctttga
     3661 cggactcaat gaaccgtgac ctacctgcga ccaatcagat taacttctta tctacgatgc
     3721 tagcgtctat ggtagggttc cttttgatgg cagctgagcc tgcaaaagaa ggaggcttct
     3781 tgacagcctt cacgggaaca aaaggtcttt tgacagcatt tattgcagcc tttgtgacag
     3841 ttaatgttta caaggtctgt gtgaaaaata atgttactat tcgtatgcct gaagaggttc
     3901 caccaaatat ctctcaagta tttaaagact tgattccatt cactgtagcg gttgttctcc
     3961 tttatggatt tgaactcatt gttaagggaa ctcttggtgt aaccgttgca gaatcaatcg
     4021 gtacccttct tgctcctctc ttctcagctg cagatggtta ccttggaatt acacttatct
     4081 ttggtgctta tgccttcttc tggtttgttg gtatccacgg tccttcaatt gtcgaaccag
     4141 caatcgctgc tatcacttat gccaatatcg atgccaactt gcatcttatc caagctggac
     4201 aacatgcaga caaagttatc acttctggta ctcaaatgtt tattgttacc atgggtggaa
     4261 caggagctac attgattgtt ccattcttgt tcatgtggat ttgtaaatca gaacgtaacc
     4321 gtgccatcgg acgtgcctca gttgttccaa cattctttgg ggttaatgag ccaatcttat
     4381 ttggtgcacc aatcgtattg aatccgattt tctttgtacc gttcattttc gctccgattg
     4441 tcaacgtttg gatctttaaa ttctttgttg acacattgaa catgaactca ttctctgcca
     4501 accttccatg ggttactcct ggtccgttgg gaattgtact cggtactaac ttccaagttc
     4561 tatcatttat cttagccgga ctcttggtag ttgttgatac tatcatttat tacccatttg
     4621 ttaaggtata cgatgaacaa attcttgaag aagaacgttc tggtaaaaca aacgatgctc
     4681 ttaaagaaaa agtagctgca aacttcaata ctgctaaagc agatgccgtt cttggaaaag
     4741 caggcgttgc taaagaagat gtagcagcaa acaataatat cacaaaagaa actaatgtcc
     4801 ttgttctttg tgcaggtgga ggtacaagtg gcttgcttgc caatgccttg aacaaagcag
     4861 cagcagaata caatgttcca gtcaaagcgg cagcgggtag ttacggtgct caccgtgaaa
     4921 tgttgccaga gtttgacttg gttatcttgg ctccacaggt cgcttcaaac ttcgatgata
     4981 tgaaggctga aacggataaa ttgggcatta aacttgcaaa aactgaagga gctcaatata
     5041 tcaaattgac acgtgatgga caaggtgctc ttgcatttgt tcaacaacag tttgattaat
     5101 aaatttgtaa aagttatttg agacgagatt attctttcaa ttccttataa tattgttaac
     5161 catatcattg catttcattt ccttggtttc cggaactcag aatatcgtct caaatgcttt
     5221 ttttgaaagg atttatagtt atgactaaaa cacttcctaa agattttatt tttggtggtg
     5281 ctacagctgc ttatcaagca gaaggtgcga ctcacacgga tggtaaaggc ccagttgcat
     5341 gggataagta tcttgaagac aattattggt acacagcaga gcctgcaagt gatttttacc
     5401 ataaatatcc tgttgattta gaattagctg aaaaatatgg cgttaatggt attcgtattt
     5461 caattgcctg gtctcgtatt ttcccaactg gctatggcga agtaaatgaa aaaggtgttg
     5521 aattctacca taatcttttt gccgagtgcc acaaacgtca tgttgagcca ttcgtgactt
     5581 tgcaccactt tgacacacca gaagttcttc actcagatgg agatttctta aaccgcgaaa
     5641 atatagaaca ctttgtagat tatgccgctt tctgttttga agagttccca gaagtaaact
     5701 actggacaac tttcaatgaa attggcccaa ttggagatgg tcaatacttg gttggtaaat
     5761 tccctccagg cattcaatat gatcttgcca aagtcttcca atcacaccac aatatgatgg
     5821 tttctcatgc acgtgctgtg aaattgtata aagataaagg ttataaaggc gaaattggtg
     5881 tagtacatgc gttgccaacc aaataccctt atgatccaga aaatccagca gatgttcgtg
     5941 ccgctgagtt agaagatatc atccataaca aatttatctt ggatgcaacc taccttggac
     6001 actattcaga taaaacgatg gaaggggtca accacatcct agctgaaaat ggtggagaac
     6061 ttgatcttcg tgatgaagac ttccaagcac ttgaagcagc taaagatttg aacgatttcc
     6121 ttggtatcaa ctactacatg agtgattgga tgcaagcctt tgatggtgag actgaaatca
     6181 ttcacaatgg taagggtgaa aaaggaagtt ctaagtacca gattaaaggt gttggtcgcc
     6241 gaatcgcacc agattatgtg cctagaacgg attgggactg gattatttat cctgaaggct
     6301 tgtatgacca aatcatgcga gtgaaaaatg attatccaaa ctacaagaag atttacatca
     6361 ctgaaaacgg tcttggatac aaagatgagt ttgtagataa taccgtttat gatgatggtc
     6421 gtattgatta cgtgaagaaa cacttggaag ttttatcaga cgcaattgcg gatggtgcaa
     6481 atgttaaagg ttacttcatc tggtcactta tggatgtctt ctcatggtca aacggatacg
     6541 aaaaacgtta tggactattc tatgtagact ttgacacgca agaacgctat cctaagaaat
     6601 cagctcattg gtataagaaa gtagcagaaa ctcaagtgat agagtaatat tacttgaaaa
     6661 tgtaaaaaat tgttatccga tactttagaa aaagtacaac acttatttag accaggcttt
     6721 gaaaaggtat atattgttag tttcgaagat tgtcctatga ttcctgagct tgaagctacc
     6781 ccgttgctaa aatgtggtaa atggtatgtt tcaactggaa aagagtggat ttgccattct
     6841 gatttagagt tatcagcatt tgaatgggaa tttttacaat cactcgatgt agaaatacgt
     6901 gagactattc acttcgaggt aaattatttg cctttccaat aataaaaaga aaggaattag
     6961 ttgtgactat cgaattaaaa aacgagtatc ttactgttca gtttaaaaca cttggtggtc
     7021 aactgacttc gattaaagac aaggatggga tagagtatct ctggcaagct gatccaaatt
     7081 attggaatgg acaggcgcct attctatttc ctatctgtgg tagtttacga aatgattggg
     7141 ctatttatag acctcaagaa agaccctttt ttacaggtct tattcgtaga catggttttg
     7201 ttcgaaaaga agaatttatt ctggaagaag tcaatgataa tagcgtgacc tttagtatta
     7261 aaccaaatgc tgagatgctt gataattacc tatatcagtt tgaactgaga gttgtttata
     7321 ccttgaatgg taaatcaatt agaaccgaat ttcaggtaac gaacttggaa atggaaaaaa
     7381 caatgcctta ctttattgga gcccatccag cattcaatag tcctttagta gaaggtgaaa
     7441 actatgagga ttattatatt gaatttagtg aggttgagtc ttgctcgata ccaaagagtt
     7501 ttcctgagac aggattatta gatttgcaag atcggacacc ttttttggaa aatcaaaaaa
     7561 ttctcgattt ggactactca cttttttccc atgatgccat tacacttgac cagctcaagt
     7621 ctcgtagcgt aacgcttcgg tcaaggaagt ctggtaaagg tcttcgagta gattttgatg
     7681 attttccgaa cttgattctg tggtctacaa ctaataaaag tccattcatt gctttggaac
     7741 cttggagtgg tctctccact tcacttgaag aaggtaattt cttagaagat aaaagacagg
     7801 taacgaaaat ttcacctcaa gaaacttcta gaaaaatcta tgatattact atccttaatt
     7861 aaaatttgtt attatttgag tcttcaataa gatttgaata ctcttacctt gtgatgatag
     7921 attggtcttc tccgtgccag tcttacatcg caaggttttt aagttttttg tgggtctatg
     7981 tcaactgtag tgggtgacga aaagctaata tctaaagaca gtctggtagt aaaaaataag
     8041 attatttact acttcttgat ttaaaataat agtggagcca ttaatttgtg gcaaatttaa
     8101 tttaatattt tgcctcaaat tagccacact ttttatagaa ctaagaggaa aaatgaaatt
     8161 ttcttcctat taatatagtt gtttgaaatc ttaaatatag cgt
//
LOCUS       DANKAZ010000103         8203 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000103 DANKAZ010000000
VERSION     DANKAZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(451..1233)
                     /locus_tag="PWY67_002351"
     CDS             complement(451..1233)
                     /locus_tag="PWY67_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058573.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1298..2134)
                     /locus_tag="PWY67_002352"
     CDS             complement(1298..2134)
                     /locus_tag="PWY67_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2058574.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="PWY67_002353"
     CDS             complement(2152..3561)
                     /locus_tag="PWY67_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2058575.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="PWY67_002354"
     CDS             complement(3575..5398)
                     /locus_tag="PWY67_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2058576.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6241..6567)
                     /locus_tag="PWY67_002355"
     CDS             complement(6241..6567)
                     /locus_tag="PWY67_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058577.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6613..6975)
                     /locus_tag="PWY67_002356"
     CDS             complement(6613..6975)
                     /locus_tag="PWY67_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDL2058578.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7012..7227)
                     /locus_tag="PWY67_002357"
     CDS             complement(7012..7227)
                     /locus_tag="PWY67_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2058579.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7354..7689
                     /locus_tag="PWY67_002358"
     CDS             7354..7689
                     /locus_tag="PWY67_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2058580.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7765..>8203)
                     /locus_tag="PWY67_002359"
     CDS             complement(7765..>8203)
                     /locus_tag="PWY67_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058581.1"
                     /translation="VFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINT
                     VCERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGA
                     GIDETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         2536 a         1735 c         1268 g         2664 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
     6121 cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact
     6181 ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa
     6241 tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg
     6301 atagtactca atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta
     6361 tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta
     6421 ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc
     6481 tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa
     6541 cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc
     6601 ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac
     6661 ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat
     6721 aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat
     6781 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
     6841 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
     6901 ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca
     6961 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt
     7021 tactttatta aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag
     7081 catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
     7141 ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta
     7201 tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa
     7261 tatgatgtat ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa
     7321 cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc
     7381 attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc
     7441 tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc
     7501 ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc
     7561 gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt
     7621 cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg
     7681 aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa
     7741 gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac
     7801 tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc
     7861 tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa
     7921 aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag
     7981 agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt
     8041 atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag
     8101 atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg
     8161 tttttccttg ctgagttctg atcgggaatt ttgaggaaat act
//
LOCUS       DANKAZ010000104         8148 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000104 DANKAZ010000000
VERSION     DANKAZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            94..1248
                     /locus_tag="PWY67_002360"
     CDS             94..1248
                     /locus_tag="PWY67_002360"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL2058582.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="PWY67_002361"
     CDS             complement(1270..2022)
                     /locus_tag="PWY67_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2058583.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="PWY67_002362"
     CDS             complement(2386..5052)
                     /locus_tag="PWY67_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2058584.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="PWY67_002363"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="PWY67_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2058585.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="PWY67_002364"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="PWY67_002364"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL2058586.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2368 a         1694 c         1454 g         2632 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtc
//
LOCUS       DANKAZ010000105         8148 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000105 DANKAZ010000000
VERSION     DANKAZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(77..961)
                     /locus_tag="PWY67_002365"
     CDS             complement(77..961)
                     /locus_tag="PWY67_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2058587.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1056..1493)
                     /locus_tag="PWY67_002366"
     CDS             complement(1056..1493)
                     /locus_tag="PWY67_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058588.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(1710..2621)
                     /locus_tag="PWY67_002367"
     CDS             complement(1710..2621)
                     /locus_tag="PWY67_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2058589.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(3078..3506)
                     /locus_tag="PWY67_002368"
     CDS             complement(3078..3506)
                     /locus_tag="PWY67_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058590.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(3534..4172)
                     /locus_tag="PWY67_002369"
     CDS             complement(3534..4172)
                     /locus_tag="PWY67_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2058591.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(4176..6158)
                     /locus_tag="PWY67_002370"
     CDS             complement(4176..6158)
                     /locus_tag="PWY67_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL2058592.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            6338..7003
                     /locus_tag="PWY67_002371"
     CDS             6338..7003
                     /locus_tag="PWY67_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2058593.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT         2601 a         1540 c         1233 g         2774 t
ORIGIN      
        1 gacaaaagtc ttgtcacagt cccttatccc gaacaagcgg tgttctcccg ccaactcctc
       61 ggtcacaacc tccattttaa caataaacct gtaaatgtga gggcaatact ttgattgtca
      121 caggtaattt atccccttca tctccatcaa cattcgtcgt caattcttcc tcatcagcta
      181 atgacacaac taattgctca caagtaaggt aggctagatt tttcgtagat tcatctactc
      241 cttttaatag gtctggcaat gttttaagcg tatcgatcaa agaagaatct tttaaataaa
      301 ccaaatggag cttaccatca tccacttttg catttggaag taatgtttca aaaccgccga
      361 ctgagttagt tagtcctatc agcaaggtac tgctctcaat ttcttccttc tttccatcaa
      421 cttctacgtg aaacgagtag gattgtgtgg aagctaactg tttgattcct gacatgaaat
      481 aagctagttt tccaaatttc gtcttcttct ccgcatctac atcattgatt gattcaggga
      541 tagagccaat tgcgacgaca ttcataaaat agtcgtcatt tatttttcca atatccaatg
      601 gcttgactga ttcaatcgaa aaatgattga tcgcttcctc aggttctagc ggtatcccta
      661 atgctcgagc caagtcattc accgtaccca atgggaaaaa tccgaaattt ggccgatgtt
      721 cttgttcagc gattccactg attccttcgt tgactgttcc atctccgccc atgacaaaca
      781 cactatgata cccttccgta gccgcttcgc gagtaaagtt ttttgcatcc cctgcttttt
      841 ttgtatgcaa gacgaccact tcatcaaaca ccgattccaa ttttgctatc gctaattgct
      901 caaattcctt tgcttgttca ccgcctgaac tggggtttac cactaataaa acttttttca
      961 tcactttctt tcccttctga ttgatttctt taatataacg atagcataaa gaaaaaaatt
     1021 aggctgtgat gaagtactgt tcatgaaaca ttcatttatt ttttgacaag ataaaacatc
     1081 catactatga aactgaggaa aattaaaaca atgatatagg aaataaaaga aaatgatagg
     1141 cattgtatac ctaataaagt aaaaatctta ggtggcagat aaaaacacaa aaatagcaag
     1201 agaatgtaat agcgtttctt ccactttttg ttatcagacc ttatagcgat cagaggaata
     1261 ccaaatgcat aactgaaaaa ctcttgatgt actgttaata tgtcacggcc ttgaataata
     1321 aatgcgacaa tccccgctat aattagtgtg cctaacacca aaacggaaaa ttgatgaata
     1381 tattttatat acaaatgatc cattgtactt cctgcctttt tatttgtgag aaaaacaaat
     1441 ccgtatacgt gaataacttc tagaaataaa agaaatttgc aaaaccactc catttatcta
     1501 cttctacgtg cattttttga tattgttttc tttatttaag attatacaga aaaatatcta
     1561 gggtcgctgg attctaagta aaaaaaattt ttaaaataaa aagaactaaa tcaagtagta
     1621 attaaactac tgttgattta gttctaataa aaagatttag atgaacagtt ttattgattt
     1681 aaatagttca tattttttat ttataccgct tatttactac ttgctgtact agaatctgaa
     1741 gtgaatgtac tcaaaacatt ttcaaagtct ttgtctttga tttttacatt agcgtctttc
     1801 aatacttcgc caattacttt attctggaag gtactgtcac ttaattttgt gtctgttgca
     1861 atttctttca attctttttt gtatttatcc atgtcgttgc ctttgttttg gtttttcacc
     1921 attttaacca cataatattc cgtagtataa gttgaagcat tggtagaagt gatcacatca
     1981 gaaacttgac catctttcaa cttaaaggct gcttctttga cttctgccgg aaccgttgta
     2041 gtagtggaat caaatttcac ttttccgcca tcttcttttg tcgtagtatc agttgacttg
     2101 tctttggcta atttagagaa atcgtctccc ttatcagcag ctttcttcac atcttttgca
     2161 tcatcttcac ttgctacttg gataatttga gcttccactt ctggatggaa agattcccaa
     2221 gccgttttta aatcttcatc ggtcaatttg atatgtttct ttagaccttc ttgaaaggct
     2281 agactttgtt tgatactgtc tttgaatgtt tgttctgtgt aacctgcaga tttcaattgt
     2341 gaatcaaatg aatcacccaa ttgtttcttt gtttgatcat attgagaatc gatttctttg
     2401 gttgatacgt catctccata tttatcttca aatacttgat aaacgatcaa ctgttgcaca
     2461 gcggaatctg ctccttgcat ttttttcaag ctatccatta cttcttgttc gctgattgta
     2521 ccgcctttat aactaacggc tgttttttca ccggcattcg tacagcctga caataagcca
     2581 gtgcctagta cgatggcaag tgctaaaaat ctttttttca tttatcaatt ctcctacttt
     2641 tcgttttttg cgaattgcta aaaacaaagg tgttcaagat gaaaatcatc aagaacacct
     2701 ttactaatct gctcgatacg tcctgaatag atataaggct acacaatcca gaaagttcgc
     2761 tcagcagaat aaatgtcaag tagcacatca caccttaata tactacttct tttgttaccg
     2821 tttttcaagt ctatcttgcg ttccgtattt aacgtaacta attcttagaa caaatgttat
     2881 cgattttgtt tttcttcttc cgctgctggg atattcaaat cagacacttt tatgttcgat
     2941 tttccagaca tttttttgtt gtttttatta tgatatattt ttatacaact aaaaatgaat
     3001 atattaaaac taccaaaaat aacttttaac gctgtttcat tatatgtttt ttctatttta
     3061 tataaacttt tggatactta ataccaaccg gttggattgt accaacatga gaatgtgtca
     3121 gttctatttc ctgtcgcact tgcataggcc catactcctt ttgttgcatt tgctgatcga
     3181 atagagtttc caactttcgc ttttgcaccg tgacgattat agacacgact atagtggtta
     3241 gaatgacctg tttttctcgt acaagttgta tcactataat aaacccatct tccagagcta
     3301 tgattaattg ctgttctaga aatttcccgg tctactggta aatttgactt aataatatta
     3361 cctttagcat caaaatgaat ctctcctggc tttaaagatt tgtaatagat ttcaccgtga
     3421 actgaatttg cttccacttc agatgacata gcgtttcctc caacaaaacc tatccctagt
     3481 gcacaaataa taactgcttt tttcataaaa cgacatcctt tcctttttga tttttaaagc
     3541 atgatcttct tgtttcctat tttggtcaga tatggatcat gcgtgactac gataactaac
     3601 gtaccttggg aagcttgttg cgataagatt tcaactactt tatccttatt tcttttatcc
     3661 aatgacccag tcggttcatc ggctaaaatg attttgctat tcttcaataa gactctcgca
     3721 caggcgattc tttgcttctc accaccagat aatgtgtaga taggcgtact tagcttcttg
     3781 tttatattta cttttttcaa tgcctcatac tttgctgttc gcttatctga aacatttaaa
     3841 ttcgaaaact tcaatccaat atctaaatta aaatcgattg tttcatattc aatcaaccca
     3901 aaatcttgaa aaagaaaaga tatggtattt ctttttatca agcgcttctc tctcccagtt
     3961 aattgtttcg tctcttgatc gaacaaatag aattgtcctg aactgaactg atcattcaat
     4021 cctaatatat tcaataacgt acttttacct gctccacttt ctccaacaac agtcacaaga
     4081 tccccagcat ccgcagtgaa acgatccaaa tgaagaatcg ttttgtttcg aatgattttt
     4141 ttcgcacctt ttatcactat caacggctcc aattgttaac ctccattcat cagcaataac
     4201 tgacttttct tttctgtaga caaaataaaa acaactgaaa gaataaaatc aatgaagcta
     4261 aagaaaaaaa tattaaaaag atatttaggc tctctttcta taagaaaaag gaagattaca
     4321 cttgtcgttc cccatttgat gaggttcatc atgaagaaat ctttatacgt ttcaaagagc
     4381 gaataaccac ttgaacgaaa gatagcgatt ttccgacaat tgtagacaaa atatatgcat
     4441 aacatgacat agctcaacaa gaagaaagaa aagaaagtga gtaaaaatat ctgtatcaag
     4501 taatgaaatg atctgcttaa cactttgaca gctatctctt cagtctgagt atacggaaat
     4561 gataactgca agttatcacg taactgactt tcttcaatca gctcaaataa cctttctttc
     4621 gacaaagagc ctatcttgat ctttaacggt ggatagattt ctccgctgag aatatttctt
     4681 tccaaataca tactgttttt caatgtcagt atttcaacaa ccggataatg ttcgatccag
     4741 gggatagaag gaataaatgt ataaatgggt tgattgtttt ttataaatag aaatttcggt
     4801 tctttttcac tgatttcttg ataatattct gttatttgcg ccaggtcatt ttttaaatct
     4861 gtatgtctat atcgttcagg gatcaatata acagggtgcg tttctttttc ggttatttgg
     4921 atcaaccttc cagagacatc aatcaaagga aatttcttaa gataattcag atttattttt
     4981 atccctctgc caaactgttc attttcttca ttcaagtaat ctgatacatt gactaatata
     5041 cttccgtttt tattcaaagt ttcatataat tcctgatctg ctgctgatgc aaaacgattg
     5101 gaataaataa aatccacgtg gtttttcccg atggttactg gataaaatac atgatagtcc
     5161 tcaccagcga tttcttttgg tggttcatgg gaaacagcta actgtgaaga agaaaaaacg
     5221 atagttgcta attcgacatt ggcaacggtc aaaagaagag aaaacaacaa tccaaatgta
     5281 aaaagaatgt attggatata gttgaacgct tctcttaacg aagcgatttt ttcaactaat
     5341 aaaagagaaa tcaaagagat accatatatt ccgctgaaca agaaaacgta catctggaac
     5401 aaataaagaa attgctcctt ataatacata aaaataaaac caccaatgat tcctgcaatt
     5461 atgaatgtaa taatgatttt ctttttcccc aataaacacc acaatacttg atgtaatgaa
     5521 aagtcattat tcaacaataa actgatttct ttattcatca ataagaaaaa ggatgataac
     5581 aaaaggaata aaagaatcaa tccaatagtt aaatacactc gataattata tacgtctgta
     5641 tttgaaataa ccgaatacga atagctattt tcaaagtctt caaaaatatt agtggcataa
     5701 tcagtgtgaa atgcttgatt gaatcgatca gtgaatagtg aaatgaaccg ttcataggct
     5761 tctttttctc tcgtcatcaa gaaaaattca ccttcaaaat cttttatttt gatcgattct
     5821 tcaatggacg ttacgcgaaa ggaggtatta gaatagggta ccttctcgtg agaagatttg
     5881 acatcccctt gataatacaa tgtcatttca agctttggct gtatcttgaa gttccaagat
     5941 gtatctactt cattggattc atttactgtt tttttcatat aagaaagctc agtttctgat
     6001 gagacatttt caaataccat gattagctgc tcatattctt cttgcccaat aaaatgttgt
     6061 ggcaatcgaa tcggagtata agtatctgtt tgatggaaaa attcatttgt ccgatagagt
     6121 acctgtttat ttatactaac caaggcaaaa ccactcatga taccggtcac tatgattgtg
     6181 agtaaaactg tgaacttttt tttgattatc accactctcc cttacttaaa tgattctttg
     6241 ataaaatcat accgtttttc atcaaaaaaa cgtatctatc aagtgtatag gtcatgtaga
     6301 ataaatctat atactgtaga atcgaaagga gaagaagatg aacaccggag aaaaaatacg
     6361 aacttaccgc agaaattgta agcttacgca aaaagaatta ggcgaaaaaa ttggtgtttc
     6421 cgataaaacg atttctagtt gggaaaattc tcgtacaatg ccagaccttg aaatgctgtc
     6481 acttttacat catactttag gtctgccgat tgattttcct tctattgaat cagctaccga
     6541 atcaatagaa ccaacattgc cagatgaacc tcttgttaac aagaaatctt attcatcaaa
     6601 acaacccttc tcactcaaaa agacactatc tttctttctg ctcgtctttt gtttggtcta
     6661 tctttttctt atcaattatc aatacaaaaa tgaaacgatc gatcgattca atcctttttt
     6721 gaaaccacaa atcggctata ccacactccc tgtaggatac gtggacaatt ccatcgaacg
     6781 ttgggtatgt aatgatatct ttggccatgg tacatggctg tcttttcacg ctggagaggt
     6841 agaaaaagag aaaaaccatg ctgtaatcaa acacaaagga aaacacgtca tgaacgttcg
     6901 cctgctcact tgggaagcca ttccctcaga ttaccaagaa atcctcagcc aagactatga
     6961 cgacgcctta aaaagaagcc ctcaggagcc agataggcat taagtaggag atgaacgtag
     7021 agatagaaaa aatcaatgca catgatctta tccaaaaaac aggggtacaa attaattgaa
     7081 aaagccttga gatgaaagtt cctaaataaa aaagttcaga atgataggta tttctggctt
     7141 atagtataat atttacgcca cagcctccca atgattcttt ttggaatcat aatcaaggat
     7201 ggacattcac aaaaatattt tgctcattcg atccgaatag attaacgacc aatctagtaa
     7261 gtaatataga ttggtacgta gtcaagctgc acataattta gatcaatttt agttcttttt
     7321 tatatttcta tgatctgctc atttactttt atatgatggc ttacagtaac ataaataaat
     7381 atgataatcg ctggtagttt atccaagagt actcaataaa atacaaaatg gattgttcaa
     7441 gcgcctatca taatgataaa atgaaacaaa taaacgatac aaggaggaag agactttgag
     7501 taatggcaga acaatttttt cattgtaaaa attgttcagt tgttaatcta tgggatttcg
     7561 gtagcatggg cattttttta tagaggaatc acaccaacct ggccgtttta tctcttgtta
     7621 gtgggaatat tagtaggaaa ttttgcccct cttcactatc gtacgaactt ttttccaact
     7681 gattctggcc tttttcttaa aacaaagacg cgaaaattag aaaacattat tgaaatgaca
     7741 attacctgta taatcatttt cttagttata ttcctaaacg tcttctaata agaatctctt
     7801 aaaattagct tgttacagtt agttacctag tttatataac acagttatct tatcttcctt
     7861 atcaacatct tttgccaact ctttgtctta aattcgaaac atataagcag agctacgaac
     7921 accaagactg tttcggctct gttttttgca tcaaattcac tcttatatag ctgataatct
     7981 cggctcttta aaagaaatta ttttcgcttc attttattat aaaaaggcta ggaaccttga
     8041 tgttatcagg ttcctagcct ttatttaagt attaccccac agacccttcc atatagattt
     8101 ttaaattaca cactagttta aactaaaaca aacaaggaaa aacttacc
//
LOCUS       DANKAZ010000106         8075 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000106 DANKAZ010000000
VERSION     DANKAZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..800)
                     /locus_tag="PWY67_002372"
     CDS             complement(<1..800)
                     /locus_tag="PWY67_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2058594.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPR"
     gene            946..1749
                     /locus_tag="PWY67_002373"
     CDS             946..1749
                     /locus_tag="PWY67_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL2058595.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1927..2874
                     /locus_tag="PWY67_002374"
     CDS             1927..2874
                     /locus_tag="PWY67_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL2058596.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2942..3235)
                     /locus_tag="PWY67_002375"
     CDS             complement(2942..3235)
                     /locus_tag="PWY67_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL2058597.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3263..3928)
                     /locus_tag="PWY67_002376"
     CDS             complement(3263..3928)
                     /locus_tag="PWY67_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL2058598.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4068..4700)
                     /locus_tag="PWY67_002377"
     CDS             complement(4068..4700)
                     /locus_tag="PWY67_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2058599.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4707..5774)
                     /locus_tag="PWY67_002378"
     CDS             complement(4707..5774)
                     /locus_tag="PWY67_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2058600.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5771..6502)
                     /locus_tag="PWY67_002379"
     CDS             complement(5771..6502)
                     /locus_tag="PWY67_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL2058601.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6671..7150)
                     /gene="greA"
                     /locus_tag="PWY67_002380"
     CDS             complement(6671..7150)
                     /gene="greA"
                     /locus_tag="PWY67_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL2058602.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7286..>8075)
                     /gene="mltG"
                     /locus_tag="PWY67_002381"
     CDS             complement(7286..>8075)
                     /gene="mltG"
                     /locus_tag="PWY67_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL2058603.1"
                     /translation="NMTLDEIGKQLQEGGTSEPTKVADGKIAIPEGYDIDQIAERVAK
                     VTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEGYLFPATYDYYTST
                     SLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEGVKENDRKNIAQVF
                     FNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTGYGPGPFDNPSEEA
                     IKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         2394 a         1665 c         1264 g         2752 t
ORIGIN      
        1 cgaggatata tcttttcaat ctcgcttagt agctgatctt caaaatcaat atgtccgtcg
       61 aaaataatta tcttttcgct atactgccaa atatggacat gatgaacatc ctttacccca
      121 tcgattgctt caatttcttg ttccattttt tcatagtcga tagcaggtgc actttgtatc
      181 aaaatagcgg cagctttttt gatcaccatt atcgcttcta tcaatatata tatcgaaatg
      241 atcagtgtaa taactggatc gatccagtag atatcgaaaa aacggatcaa aattgctccg
      301 ataatcacac ttaccgaaga cagtgtatca cttaacaaat gcaaataact agattttata
      361 ttcaaacttt catctgcttc ttgtcgtaat aataatactg agaacaaatt cgcgagcaat
      421 ccaataactg ctactgttag catcaaatga ctatttatct tttctggtga agtgaaccgc
      481 ttgaatgctt caacggttaa aaacaaggat aatatgatca atatgaatga gttgatgaaa
      541 gcactcaata tttctaaacg ctggtagcca tacgtacgtt ttttatttgc ttcttttcgg
      601 gccatttttt gtgccccgta agaaaaaata attgccaatg tatcactaag gttatggaaa
      661 gcatctgaaa caagagacaa actccctgac ataatccctc caataaattc tgataaagtg
      721 atcaccgcat tcaaaaccac tactccaaat agtttttttg atgatctctg tggctgtgta
      781 ggcactattc cttcctccat tttatttata ttggatagtc tttagacaag aataacattt
      841 ttttgatatt tcaccaatca ttcagctgtt tccttaaagc acttgtattt cacttgatac
      901 ttcgctagaa ttgcaagtga tgacactact agatgaggag tacttatgac aacaaaacaa
      961 ttaattatca gttcctttat acgattcaaa gacctaaagg aggcgagaaa tgtcccattt
     1021 tggaaagcca ttgtctatct tttagttttg agtataatta tggctttgcc aatctctttt
     1081 caagtttttc aagtcttaaa caacattcaa ttggatgggc aaaagattgc tactcgcatt
     1141 cctgatttta caatccaaaa cgggcagctt caaaccgaag aaaaagaagg attcatttac
     1201 caaacaaact ccattatctt cacatttgat ccagagggaa aacgaacaga acaggacatc
     1261 tctactgatt tgatggggaa ttttgttagt gtcgggatgt taaaaaacaa acttatcata
     1321 gctcttccta atacaggcac gacttctgct ttgttgaaca ataaccaatt agaacttccc
     1381 tacaccaatg aatctttaaa aaacttaaca ggtaagcagc tccgttcttt tttaagtgaa
     1441 gcatctatac cactttggat aaaagtcctg acatttatat tttctattta tcctagtttt
     1501 ctgaatctgc tgatcacttt gctatttacc aacattgctg cttttctcta tgcacgcttt
     1561 cgactaacaa aagctacttt tcttgattgt ttaaaaacat tgatctattc aatttcattg
     1621 cctactataa ttgcaacaat cttgatggtc tttcttcctt cttttgacac tagcgccttc
     1681 attgcttttg caggaatctt tatttttgct caagctgtca aaggatgggc aaaaatatcg
     1741 atcaattaag aaataaagac aactcgttat ttctacttca atagaccctt ttagaggttg
     1801 tgacattttt ttgtcacagc ccctttttta tttgaaaaat atttctttct caaaaaacaa
     1861 gcaaggcgct tcaaagttct tcttgtatgc gctacactta gagtaaagaa aggcgggaaa
     1921 ttaactatgt cagaaagaat catttatgca gatcgtccat ttcgtgatga atttatcgaa
     1981 gaaatccata ctatagcgcc tggttatatc tttaaaacag aattacaacc agaggatcta
     2041 caaaatgtag agatcagttt gggctggaac tcagactacc aagaaaaact attagcctct
     2101 tctaccttga aatgggtcca atctatctca gctggtgttg atcagctacc ccttgagact
     2161 ttttcaaaga aacatatttt actttcaaat ggtagtggca tccacagtga atcgattacg
     2221 gagcatgtct taggaatcat ccttgggtat tctcgtggtc tttttcaagc acaaaaggca
     2281 cagagtgaca tgaaatggtt aggttcatcg gtccattacc aatctatcca agggaaaaaa
     2341 atactaatca ttggaactgg ccatattgga aaaatgattg caaaaaaatg caaagcatta
     2401 gacatgaact gctatggaat caatacatcc ggtcatccag cagaaggaat ggaaaagact
     2461 ttttctttaa atcaactaaa ggaagttctt cctgaagtag atttcgtagt caatatctta
     2521 cctctaacgg aggaaacaac tgggctcttt gatcagcata tgtttgaaca ctttgatcca
     2581 aaatctgtat tcatcaatgt tggccgtggt gcttctgtta agacacaaga tctggtgcag
     2641 gctttaaata accagcaact ttcctttgca gcattagacg tttttgagga agagcctctt
     2701 ccacaagatc atcctctttg gaaaatggac aatgtactga ttacttccca cattgcaggt
     2761 ttgacacctg attttcaaaa gaaattaatg gctatttttt taaccaatct gaaaagctac
     2821 tttgccaccc atgagcttca aaccaatcaa gtaaaattgt ctgcaggata ttaatagctg
     2881 aaaacaatat tttcaaaaga aaaaagagaa ggtgacaaaa attgtgtcac cctctctttt
     2941 tttattgttt tttaaagtga taggttggct cctccaggtt gggatcataa tcaacaagta
     3001 aatcatatcc tttaaagaac cattcatctg cttcctcgat aaaaaaaagt gtcccatcca
     3061 tttccacttt ctttacagga tgttctggct gttcaactga cattccaatc gaaaaaccat
     3121 catgaacctc tgttttgccg tagacttttc caaaaaaacg tattccgtaa gtttctggca
     3181 gatcaatttc tgagtcaaac cacttttgtg cttttggcgt taccgttaat ttcatcccaa
     3241 caacctactt tctcttttct ttctattgat tcatcttatc gtctagaata tccacatcgc
     3301 ctgtctctcc aataaccagt agattatcat tttcacgaac atattcatct gctgcaggag
     3361 aagcgataac cgtttgatct actcttcgta tggcgactac tgttaagtca aaacgttgcc
     3421 tgaaatttaa ttcagctaat gttttgttga aaaatttagg attggagact cggatttctg
     3481 ctaaagaata tttatctgac aactctaggt aatctaaaat attctttgac actaaattat
     3541 gcgcaatcct tattcccatg tctcgctcgg gatggacgac gcgatcagct ccgatctttt
     3601 caagtactct ggcatgatac tcattttggg cttttgctaa aatatttggt acgcccattt
     3661 cctttaccat cagagtaact aagatactgg cttgaatatc ttctccaata gcgacaatta
     3721 catgatcgaa atttcttatg cctaatgatc gaagtgtcat ttcatcctgt gcatttgcca
     3781 caacagcatg agtggctata ttcatatatt cattgacccg atcttcatta ctatcaatag
     3841 ccagtacttc ttgaccagct tcgatcagtg ttcggcagat acttccgcca aatcgtccaa
     3901 gacctacaat tgcaaaattt tgtttcaccg ttttcctcct ttagtatctt cttttctcag
     3961 tataacaaag cctttacaaa aacaaaagca agcatcatta ttgtaaaaaa gccaagaaaa
     4021 ataatagtta ttttctcagc ttttgattat cctctttatc atcgttttta tttcaccaaa
     4081 ccatgtttga atgcatagat agcggcttgt gtacggtctt ccacttctag cttagccaat
     4141 atattggaaa catgtgtttt tactgttttc agtgtaataa acaattcgtc cgctatttcc
     4201 tgattacttt ttccttcaga aatcagcatt aaaatttcat tttcccgatt cgttagttct
     4261 tcatgtaata caggctcatt tcgccgactc attttttcca tcatttttgt tgtcacttct
     4321 ggttctaata ctcgctcgcc acgttgcgtt gcccttatag catcagcaat ttcatgtgcg
     4381 gtagaggtct tcaacaaata ccctgctgca cctgcttcaa tggcaggata aactttttcg
     4441 tcatcaataa aactggttac gataatgatt ttagcttctg gccaatcttt taagattgct
     4501 tttgttgatt cgataccatc catttcttcc atgactaagt ccattaaaat gacgtccggt
     4561 cgaagtgtca tcgccttctc atagccttgt cgtccatttt cagcttctcc aattacttca
     4621 atgtcttctt gaatagataa ataagaagag acgcctaagc gcaccatttc atggtcatct
     4681 actaataaaa cttttatcat ccttatctat gcctccttca tcaaagggac cttgatttct
     4741 acacttgtac cttgaccttt aaaactaatg atttttactg ttccaccaat accagctact
     4801 cgttctttga tattgttcaa tccataactt cccgttttcg tttcattcat attaaaccct
     4861 gttccgtcgt caataatacg taacaaaaga ttattgtcta ttttgtgcaa atatacctct
     4921 aattcattgg ctttagcatg tcttaatgta tttgataaca attcttgaac gattcggaac
     4981 aagtgatcct caatggaact agatagtttt acatcttcta catcccattt cagcgaaatc
     5041 tggattttat tttgcaattc cttcaataac tgctcgatac cttgctttaa acttttctct
     5101 tctaaattga ctggacgtag atgaagaagc agcgcacgca tctcagactg ggaagcgttg
     5161 atgatctctg ctaccatttt caactgcttg cgaaacattt ctggtgtctc gcttttttct
     5221 gcttgctccg tcaatgccga catcatcatc attgctgcaa acaattgttg cgaaactgaa
     5281 tcatgcagct cacgggctaa tcgatgtctt tctaattcta aaatttcttc tttggtttgt
     5341 ccatctacat attggggacg gctcgttaca atttgtaatt cactagatac ttcaatcatt
     5401 ttttctttta tcttagtgat ttccttatca atatctttga tgtataaatc gtccgatgca
     5461 cttgggatcg gaagaagcaa caatttggat tcataattgc cagaagaaag tgcacgcagc
     5521 ttttcttcga ttttcccgta ttttgtcttt tgaataagtg ataataatag aaagacaatt
     5581 aatcctacac ctaaggaaat ggctaacaaa tggaagataa gcggaacata taataaacgt
     5641 gcacgcagta attctcccca ccaatgactt tgattgctgg cataaaaata agtgaaaagt
     5701 gaaaatagaa taataaggaa agccgcaatc cccgaataaa cagctagcat cgctctggat
     5761 atttttccca tcatattcgt atcacctcga catctccaat caaggtatta gtaataatct
     5821 tcaatctacg agggttttca tcataatcac tgctgtagat tttgatttgt tcatttttca
     5881 aagaaaattg ctcttcttca aacaagacat ttcctactaa tgtcgcatgc tctaatcgga
     5941 tggctacgcc caaaggtact aagatccgtg ttctgcctat cccttttctt acaatcacaa
     6001 tattatcatc ttttggcaaa agcgtattgc ccaaatctat gatagtatca ccagaaatga
     6061 cggcgatgtt gatatcgtcc cattcataga cgtcgtttcc aatacgttgg ttaccgaata
     6121 attgctgtct tttccgctca ttattatgtg tttttaattg ctctgtttgg atcatcatga
     6181 tctgtttctt tcgccaaaaa gcatttttgg ttaaatctat ccctgaaatc tctacacctt
     6241 tcagaccaat aaataaaacg gcaaatacag ccatcatcca taaagcagga ctgttgacta
     6301 agctaaaaaa aatgaccaaa cttcctaata tcaattgaaa attttgaaat ttagtacgag
     6361 gatgtttgcg catagctaga tatatattga aaataccgaa tatcactaaa agcaaaagtc
     6421 cagtattgtt tacgatttgc caaacagcaa ataacagcag tagtgcttca actaccacaa
     6481 aaaaacgcca agaactattc atcgctcttt ctccctcctg acacattctc atttcatcat
     6541 acctattttt ttaactgttg gctataggac tttagatgta agatcgtttg ttgaaatcta
     6601 aaatacacaa agagaaaaag atctagagtg tatcatcagt aagatgaact ctctagatct
     6661 ttcagtcaga ctacgcttgt tctactttga cgattttaac taacatatcg ccacctggag
     6721 tagcaatagt tacttcatca tttttacgtt tgccaatcaa tgcttgcgca ataggagaat
     6781 cattggaaat tttgccagaa aacggatctg cttcagcgct tccgacaatc gtgtactctt
     6841 cttcttctcc atctggtagt tcgatgaatg taactgtttt accaattgat acctcatctg
     6901 catctacacc gtcgttatcg atgatttgag caaaacgaat catattttca attgttgtga
     6961 ttcttccttc aacaaatgct tgttcatctt tcgctgattc atattcagag ttttcggaaa
     7021 gatctccgaa accgcgggca attttgattc gttcaatgat ttctccgcgt ttgactgttt
     7081 ttagttcttc taattctttt tctaattttt ctttaccttc tagagtcatt ggataaactt
     7141 tctctaccat gtgtaacgtc tccttttttc gataaaatac cgcaaaaggt aatcttttga
     7201 ggaatatagt ccaaaaaaga ttaccacgct tttcttcaaa tgtaaatgtt tttctaaaaa
     7261 tgatcaatgt ctagagcctg tttttctaag aattatttac atatttttcc actaattcca
     7321 tatgttcttc ataagtttta gcgaaataaa catttccagt gctcgtatct gctacgaaat
     7381 aataatagtc attttctgct ggttctaata ctgccttgat tgcctcttcg cttggattgt
     7441 caaaaggtcc aggtccatag ccggtattgg tatacaaatt atatggtgaa tctacagctg
     7501 tatcttcata cgtcactaat tctttgtgtt cacctaatgc gtataaaaca gagatatctg
     7561 actgaagtgg catatttgct ttgattcggt taaagaagac ttgtgcaatg tttttacgat
     7621 cattttcttt tacgccttct ttttcaacta gagaagctag agttaaaact tcttgtacag
     7681 tcaaattttt ctgcttgata gctgaataat agttttgcat gaccgtattc gtcttgttca
     7741 ccatttcaat cactagatcc ttaagcgaag tactcgtgta ataatcatat gtggctggga
     7801 ataagtaacc ttccaaacgg tattttgtgt tctcagcttt agatgcactt tccaacagtt
     7861 ctgggaattt ttggtgcaac tcgttaaaga aagtctcatc attcactaaa tctaaaaatt
     7921 ctttcttgtc tttaccagtt accttcgcta cccgttcagc aatctgatcg atatcgtacc
     7981 cttctggaat ggcaattttc ccatctgcaa cttttgttgg ctcagaagta ccgccttctt
     8041 gcaactgttt gccaatctcg tcaagagtca tatta
//
LOCUS       DANKAZ010000107         7879 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000107 DANKAZ010000000
VERSION     DANKAZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..395
                     /locus_tag="PWY67_002382"
     CDS             <1..395
                     /locus_tag="PWY67_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058604.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="PWY67_002383"
     CDS             398..1228
                     /locus_tag="PWY67_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL2058605.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="PWY67_002384"
     CDS             1244..2095
                     /locus_tag="PWY67_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2058606.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="PWY67_002385"
     CDS             2092..2451
                     /locus_tag="PWY67_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL2058607.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="PWY67_002386"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="PWY67_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="PWY67_002387"
     CDS             2624..2929
                     /locus_tag="PWY67_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058608.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="PWY67_002388"
     CDS             2929..3240
                     /locus_tag="PWY67_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2058609.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="PWY67_002389"
     CDS             3237..3410
                     /locus_tag="PWY67_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058610.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="PWY67_002390"
     CDS             3407..3823
                     /locus_tag="PWY67_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDL2058611.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="PWY67_002391"
     CDS             3823..4491
                     /locus_tag="PWY67_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL2058612.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="PWY67_002392"
     CDS             4488..4865
                     /locus_tag="PWY67_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058613.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="PWY67_002393"
     CDS             4862..5161
                     /locus_tag="PWY67_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058614.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="PWY67_002394"
     CDS             5151..5309
                     /locus_tag="PWY67_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058615.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="PWY67_002395"
     CDS             5403..5819
                     /locus_tag="PWY67_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2058616.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            6171..6575
                     /gene="tnpA"
                     /locus_tag="PWY67_002396"
     CDS             6171..6575
                     /gene="tnpA"
                     /locus_tag="PWY67_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDL2058617.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            6592..7740
                     /locus_tag="PWY67_002397"
     CDS             6592..7740
                     /locus_tag="PWY67_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2058618.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT         2916 a         1201 c         1725 g         2037 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 gaattttaac agaattaggc agaagtcgcc catcctctat aggtgggtga tgaatgccgt
     6061 tcggtatgag ggttaccgtt ggtaactcga agaccgacaa ctaaggcgta ttgccttttt
     6121 attttgttcc atgttacaat cagtctatac aagaaatgag ttgatataac atggaattag
     6181 atacaaataa tcattcagta tttcttcttt attaccacct tgttttagta acgaaatatc
     6241 gtagacaagt gattgatgat gaaatatctg attatgccaa aactactttt gaaagaattt
     6301 cagaatcgta tcacattact ttagtggagt ggaatcatga taaagaccac gttcatatta
     6361 tgttcaaagc tcaacctaaa acagaattga caaaattcat caatgcctat aaaagtgcca
     6421 gctcaagatt gataaaacgt gactttccaa gagtcaaaca atttctttgg aaagaaatgt
     6481 tctggtctaa aagtttttgt cttctgacaa ctggtggcgc accaattgat gtgattaaga
     6541 agtatatcca aaatcaagga aacaatcata aatagaaagt aggtgaactt tatggaacaa
     6601 ctaaaagcat acaaattcag aatctatcca acagaggaac aagaaatctt ctttgccaaa
     6661 tcttttggtt gtgtccgtaa ggtctacaat ctaatgcttg atgaccgaaa aaaggcgtat
     6721 gaagaagtta aaaatgattc ttctaaaaaa atgacttttc cgacaccagc taaatacaag
     6781 aaagaatttc cgtttttgaa agaaattgat agccttgctt tagccaatgc ccaacttaat
     6841 ttagataaag catacaagaa tttttttcgg gataaatccg ttggatttcc acgtttcaaa
     6901 agtaagaaaa atcctgttca gagttataca acgaataatc aaaatggtac agttgctttg
     6961 attgatagta aattcatcaa agttcctaaa ttgaagtcat tagtaagaat taagcttcat
     7021 agacaaccaa aagggattat caaatccgct acaatatcac gtcattctag cggtaaatac
     7081 tatatttctt tgttgtgtaa agaagaaatt atcgaattac ctaaaactaa ttccacaatt
     7141 ggtattgact taggtattat ggactttgcg attctttctg atggacaaaa aattgataat
     7201 aataaattca cgtccaaaat ggaaaagaaa ctaaagcgtg aacaacgcaa attgtctagg
     7261 cgtgctttat tggctaaaaa taaaggtacc aatctatttg aagctaaaaa ttatcaaaaa
     7321 caaaaacgca aagtagctag attgcatgaa aaagtaatga atcaacgtac tgattttctg
     7381 aataagttga gtacagaaat gatcaaaaat cacgatatta tctgtattga agacttaaac
     7441 acaaaaggta tgttgcgaaa tcataaatta gctaaaagta tttctgatgt ttcttggtca
     7501 aagtttgtga ctaaattaca atacaaggct gactggtatg gacgaaaaat catcaaagta
     7561 gataaatggt ttccgtctag tcagatttgt tcagagtgcg gacataaaga tggcaagaaa
     7621 tctcttgaaa ttcgagaatg gacttgtcct gtttgtcata ctcatcatga tcgtgatatc
     7681 aatgctagta tcaatattct gaccgaaggt ctaagactac attctatggg attggcttag
     7741 atccaatcat caagaaccgt aggaactacg gggatagctt ggtaaataag agacaccgct
     7801 gttagtaaag aaatacgcta tcaagtatgc tctattccca agaagctccc acttcaagcg
     7861 ttaagaaccg tcaggttta
//
LOCUS       DANKAZ010000108         7742 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000108 DANKAZ010000000
VERSION     DANKAZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(178..1812)
                     /locus_tag="PWY67_002398"
     CDS             complement(178..1812)
                     /locus_tag="PWY67_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2058619.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(1816..3189)
                     /locus_tag="PWY67_002399"
     CDS             complement(1816..3189)
                     /locus_tag="PWY67_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2058620.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3205..3444)
                     /locus_tag="PWY67_002400"
     CDS             complement(3205..3444)
                     /locus_tag="PWY67_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058621.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3626..6301)
                     /locus_tag="PWY67_002401"
     CDS             complement(3626..6301)
                     /locus_tag="PWY67_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDL2058622.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6484..7407)
                     /locus_tag="PWY67_002402"
     CDS             complement(6484..7407)
                     /locus_tag="PWY67_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058623.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2278 a         1524 c         1175 g         2765 t
ORIGIN      
        1 acccatcttt ttcatatcta tccccactct ttttaattct tatatacaat tatatttcaa
       61 atattcatta ataaatatcc ctatttatga taaggacctc cctaactact tctctaacga
      121 tttcaaatcg ctagagaagc gttagcgccc ctctaacgtt gtttgatata acctagacta
      181 gatcgttaac gtaaagggtg tttttaacac aaaaatagcc gtatcctttg ttttggatac
      241 ggctatttga tattgctcat tcaattgttg taccacggca tgcgtgaatt tcatcgtaat
      301 ttccaacatc tccttatggt taggatcaaa ggttattttc tctataaagg tacgatacaa
      361 ggctttgatt tgcttcttct cactctgggc aagaaaacga tccaaattag tcaatatccg
      421 ttggacatct ttgacttgaa tcggctctcc ttgttgaaag atccctaaga tttcattttc
      481 tcgaatgtgc aactgtcgtc gtttttcagt caattcgtct aagcggtctt ttaacatcgg
      541 aaacaattca ggactatctt ccaaagcagc ttcccatttt tcaattttag tcttcaattc
      601 ttcctttcgt tccagtaaca ccacgagttc ttgttccaat ggttggcttt gtcgcactat
      661 ttcttcattt aacgctgcca ccaatcgggg aagaacttct ggtagctgaa cgatctcctt
      721 taatcggtcg gcgacaaatg tttccgcttg ttcggcacga atcgaattgg catgacacac
      781 acttgctcct ttattccgaa agactgaaca agaataatat cgaatccgtt tctttgtgcc
      841 atcctttagg gtatttgtga cattactcgc tgccatcggt gcgccacatt ctggacaacg
      901 taacaagcct gtcaatacat tttcacctgt atggttccat ttgggttgtt ttccttctaa
      961 agcaaggcgt tcttgtactt tttgataatc ttcttccgaa ataatcggtt catgggttcc
     1021 tttcactaaa atcggtctcg gattcttccc cttccgtcgt ttggtttccc aattgatgta
     1081 gcgcgcatat tctaaatacc ctgcataaat tttattgcgt aagatatctt tgactgccgt
     1141 ggtagaaaaa gcattctttt tgactgtttg atatccttga cgattcaaaa aattggcaat
     1201 cgctcgatac cctgctccgc ctaaatattt tgcatagatc agacgaacaa ttttcgcctc
     1261 ttctggttca atgaccaatt gtttttgacc attcgtgtca ataccactga catagcctaa
     1321 acgtcggcct cctgcataac gctttttatc tttcactaag ctttgcattg acatttgaac
     1381 attctccgca atttggttgc gttcaaattc accgaaactc ccaaggagtt gtaacatcaa
     1441 tttacctgtc gaagtggtca cgtcaaattg ttccgagatc gaatgtaatc ctacgtcata
     1501 tcgcaataat tcctcgactg tttgtaagac atctaacata ttgcgtccga gacgggtcaa
     1561 tttccaaatc actagtagat caaaacgatg gttccgagca tcgttcatca ttcgttgata
     1621 ggcgaaccga ttttttgtgg atttcccact gattccttca tcggcataaa cttctaccac
     1681 ttcatagccc atccgttcgg caaactgtcg tccattttgt tcttgtgctt gaatactatg
     1741 tccttctaaa gcttgttctt gtgtactcac gcgaatatac aatgcgactc gtttggtttg
     1801 tatcatttcc atagatcatt cgttcctttc taaaatattt tgtgtcagtt gtcggaagta
     1861 aatcccacaa actgttttat cctttgttac ctcaatccga caaacatagt cacataataa
     1921 ttgtcgacta gagaggtctt ttagttgaag ccattctttc acttttgctt cacactgttg
     1981 gagtgaaaga aacgaagaag tagacatcgg cttttgaaga gcttgcagtt gttgcttgag
     2041 ttcttccagt gaacaattgc cctcttcaaa tgccttaatc cattgtgctt cttgtttctt
     2101 ttgttggatt actttgcgtt tttcatcttt tcgcttttcg gctaattgtt gcgttaattg
     2161 ggcaatcaag gcttgataga cgccttcgta ttgaaaaaaa gcctgtatct tttccaatac
     2221 ttctgactca accctttctg cccgaagaaa aattccttgg cagcttcggt cggtacaatg
     2281 ataataatga agcgtcttcg tcttctttgt attccattga tacttcgaac tcagatgacg
     2341 accacagaag ggacaccgaa ttttttgacg taataagtac agatacgtat cttttttagc
     2401 tgtacacctt gattggcgaa tctcttgggc ttgtaaaaag gttgctttcg agagaatcgg
     2461 ttcaaaggtt cctagatact tccccaaaga tcctcctttg atttcgccat agtacaatgg
     2521 attttttagg atactataaa tatgataggg ctgaaaagga cgttcgcgga agaaaagctt
     2581 ttgttgcgtc aatcgttgac aaagcttttt gtagccatat tttttggtca aataaagttg
     2641 aaaaatatag cgaacaatcc ttgcttcgtg tacttcttgt tggattttcc cattcactaa
     2701 acgataccca tatggtaccg cagaagacaa atattgccgt tgttgctttt tataggaaag
     2761 tccggcacga acattctcaa taatgacgtt tcgttgttgt tctgcaaaag tgaacaataa
     2821 ttgcacaaat agtttattcg ttgacgcgtg tgagaaagaa gcaggaagag gttcagaaat
     2881 cgaatacact tgaacctgat gttgttggca caactcaata aattctaagg ctattttggc
     2941 ttgacgtgcc aatcgatcat aacgccaaac atagatcacc ccctcttctt ctgacgcaca
     3001 aacccaagct ctaagctgtc gaaaagctgg acgctccgct ttttcccctg attctccttt
     3061 gtctgaaaat cgttggggaa tttgttcata tttctctaaa acatactctt tcactgcatc
     3121 atattgagcg tcattactaa ttttcgcttc ttcataaaca gacgttcgat cataacaagc
     3181 cacacgcatg cttgattccc ctctctatga atgaactaaa taccattttt tcacgactct
     3241 tctattgaac gaacgttgca caaagtgatt ttctaaatat ccttcgttct tcaacgcttg
     3301 aagagtaccc ccgatagatc cagcagatac ccctcttatt tcgaaatcca agtaaattgg
     3361 ttttgtaaaa aaggcttgat ttgaaaaatg ttcttgaata tacagaagaa cctctgcttt
     3421 tagtgtcatt ttatttttag tcattagaat tttcctttct ttcatgtttg attgaatgaa
     3481 gtaaatgaag catagatcaa gcttcattta cttccgtttg ggtttgctcg ctatcataca
     3541 gataagtaat cagttgtttt gctaaatcaa taagaaatat ttccataaca tggtgcctca
     3601 ctttcatact ttggtttatt ctaaattaca atcgaaaata cgatttgagt gaagaaggta
     3661 atagaatctc gtatgttgga agaacttgac gattcatgtg aatacgtttc gttcttctcc
     3721 ctttttctgc acacatatta cccgaatcaa gcaattgcct aaaaagatag gttagatcct
     3781 ttacttgaaa ttctcgcctt aacaagtcct caaacgggat aggaaaaata tttaccttaa
     3841 tatccccatt tttttgaatt gatacttttc cccaaatcgt actgggcgta tgtttgttgt
     3901 cactgtttgt taaaaattta tggctattca tcaccaacaa tgttttcact cggtcaaata
     3961 ccgtttgctc acttcctaaa ctagtatcta caattaactg ttcgtacgaa ttaataagca
     4021 ccgtctgtat atgctctaat cgtaaaggat agcccagtat atcttgaatt aggagggctc
     4081 cactataaat caaagctaga ttattaccaa tgcgttgctt caaagaatgt tcaggtaaaa
     4141 cagcccatag ttttttttga gcttcgacaa aagttgcttc aatttttgta atcccttgtt
     4201 caaagagttt caaaagaaat aactttatac cccaaccaaa agtattagaa gaaagacttt
     4261 taactttctc tgcgtgattc gcatcctttg taaacttgat atcagaaaat tcgatatatc
     4321 gaactttcaa accagttaat ttctcattga aataattttc caaacgctct tcagaagagg
     4381 taagggtcaa aacagaaaaa gtctcttgtg gcataagctg acaattagaa tctaatctta
     4441 gcctttcttc tcctgaagct aattgataga tcaacgagct aaagttattt tgagttgctg
     4501 ctcctaactc atctagtatt agtggaacac ctccctgatt gtttgatgct agctttataa
     4561 tggctttatt ggtagcattc atatttctaa gaaccgtagt tggatttcca aataaagagg
     4621 caataaagtg taacgtagtg gttttacccg ttgtgctctt gccctgtagg ttaattagta
     4681 aactcaaaaa agtattagcc ttcgtaacgt ttaaatagcc taacacaata gaagcaatac
     4741 caatcgacca agcaagttct aagttcgttc ctaaaacata cttatgaacc aatgcgtcaa
     4801 attccgcttc tgtaccatta gttgttaatt ggtagcatga gtttttagtt aatacagaag
     4861 gaatttcacg tccaaaagaa tcttgtaact gaaaaatcat tgcttcattt tcttttttcc
     4921 agccaagaga ttcatgttca aaaaagattg gagcattctc ccgttgaatc tgtaaaaagt
     4981 caacgacttc agcttctctt ctagctttaa tgattaagcc atgttttgct aattgtttaa
     5041 aattattagt ttgtaaacat ccaccttcta tttctatttc atcccaacaa tgatcgacat
     5101 aataggcaag aacaagtgta atgacttttt gttcgatatt tttgcgtatt tgcttaataa
     5161 acatcggaca agcaactttt tcttcttgac cttctttcac caaaaatact ccttcttgat
     5221 tcagtttaaa ctggtcaaaa taatattctt gattggattc taatgattct acagaaggat
     5281 ttacaggttt tggcaacgga tattttttta caaagtcttg aatatcttta tttttatccg
     5341 aagcatgcag acaatgaaat cgaatcttat tatcagccgt aacagacaaa caagaaccat
     5401 tagaattatt cgtgtgtttt cttaatggac attcgtcaaa tataagtaag gtaactcctt
     5461 tatgatcgcc ctcttttatg gtataagtta attcaggata gtgagctaac cattgtttat
     5521 agtctgcttg aataaaaggt aacacttctc taaaagatgg atggcttttt actctagagg
     5581 ttgacatcat ggaattttct tcaactatct gttcaagaat tttaaaacaa ttacttctat
     5641 ctatgttatc gggaatagaa aggaaactag ataaacgatg cggacgctct tttgtggact
     5701 ttcctttgac cgctggtgta ccaactaatt tgcataatct tgaaggatta taaacagaag
     5761 tatcaatatc aacttcatct tctactccaa atcttcggtg aagtaattga agaaaattct
     5821 gaatagtaag aatcgtctcg tgattatttg gttgttcatt tatgggaaga aaaatatggt
     5881 agccattccc actatcaact tttacaaatg attctattct cttttctttc aaataacgtt
     5941 ctacttgttc cataacattt tctgcctttt tcttttcgct atctgttgca cttccctctt
     6001 tccttttagg gtcaatatca ataaatacaa accgaatttt ttcaatatca ttgtctctca
     6061 cagaatgctt tttttgtgta atcacctctt gtttaatagg attataagtt atgtttactg
     6121 taccttcaga caatttctgt ttttctaaat agttaataat tgcttgagaa tctgcagtag
     6181 aaaaaatatg agaaacggtt ttcttgttta atgtagaaaa agttctcaaa ttaacataag
     6241 aaatccccag tgtatttaac agttgtaaat gcttctgaat tgaagtgtga ttcattttca
     6301 ttaattacca ctcactttct aaaaaatttt tatttgtatg actacattac ctccaacctc
     6361 tcataatttc atgatcttca gttctacaaa aatatatccc actcaaaatc ttgacaaaag
     6421 aaaaaagttg tgattttgga aatcacaact tttttctttt acgataaatc aatccttatt
     6481 aaattattta ggaaaatatt gttcaattat ttctttatat attttctcat agtatgtttc
     6541 tgtccatcct aatgttccta taaaatcaag tcctctacat tttaaaggtt cacttaaact
     6601 attaatatag ttaataatgt tgtctatcct tttctcttta tcttcacctt caatctgggg
     6661 agcatatagt ccaatttcct cccaataata tgaatctctt tcaattccca tgatggtagc
     6721 tttggctaat ttcaaatcaa ataacgattt tagaattctt agtgtctcaa taaagtcttc
     6781 tctcttcaac tctttaatcc attgtgaatt ttctggatta tctaaaattt caagttcatc
     6841 ttcattataa gtatatttca aatttgactt gcttaactca tagataaatg aagtttcttg
     6901 actcataatt cttttaagta cttgactata agtttcgttt gactttgccc atttcgctaa
     6961 ttctaccact gtattcacta ataaatcgaa ataatgatgt tctcgttcaa tagtctcagc
     7021 attttgaaaa tgaaaatcac ctttataaac tccattcttt tccactgttt tattaatatt
     7081 ttttaaaaac cagaatgagt ttgcaaattt agtcgtactt tttcgattaa ctaagttaat
     7141 aactcttcca tgtaataata agacaaaaat acataaattt ttagcttcct tattattaga
     7201 tactaattga tcaatcatag ggagccattt tttgattttc actaattgat tgctcataaa
     7261 tctttcactg gacttttgtt caatcttaaa ttgcaaaact tctaactcat aattcccatc
     7321 ttctgattta tttttgggaa caactatcaa acttttataa agtttatcca catcaaaatt
     7381 tatctttttt gcataatttg tagccattaa tacatctcct tattcataat aaatttcata
     7441 tgacgctcct ctcaaaattt accggtagac tagaaaaaag aataaaagat acgtcaattc
     7501 acactttatt cttaaaaaaa aactggtctc tctaccggta atatcgattg agtccgcctt
     7561 tatcgacttt atcctttttt tatattaaat tttttcaccg gtagacaggg agagaaagaa
     7621 taaactaaaa agcccttaat tcttaatgtt ttcacctcct taccgttcct accgctaaaa
     7681 tttaatataa ctaaattcta taacattaga agaaatttta aatacctttg ataaaaaata
     7741 tt
//
LOCUS       DANKAZ010000109         7734 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000109 DANKAZ010000000
VERSION     DANKAZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..178)
                     /locus_tag="PWY67_002403"
     CDS             complement(<1..178)
                     /locus_tag="PWY67_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL2058624.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(191..1294)
                     /locus_tag="PWY67_002404"
     CDS             complement(191..1294)
                     /locus_tag="PWY67_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL2058625.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1384..2340)
                     /locus_tag="PWY67_002405"
     CDS             complement(1384..2340)
                     /locus_tag="PWY67_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL2058626.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2621..4555
                     /locus_tag="PWY67_002406"
     CDS             2621..4555
                     /locus_tag="PWY67_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2058627.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5012..5428
                     /locus_tag="PWY67_002407"
     CDS             5012..5428
                     /locus_tag="PWY67_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2058628.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5442..6008
                     /locus_tag="PWY67_002408"
     CDS             5442..6008
                     /locus_tag="PWY67_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL2058629.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6031..7017
                     /locus_tag="PWY67_002409"
     CDS             6031..7017
                     /locus_tag="PWY67_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2058630.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7172..>7734)
                     /gene="scm"
                     /locus_tag="PWY67_002410"
     CDS             complement(7172..>7734)
                     /gene="scm"
                     /locus_tag="PWY67_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2058631.1"
                     /translation="TDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTSTT
                     TSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTS
                     SDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNN
                     WITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2210 a         1480 c         1602 g         2442 t
ORIGIN      
        1 tattgatagc ggtagtcgct aaatcaaaaa ctagcattcc aataaaaaat aaaaaagtgt
       61 ttagccaatg tatctcatga aaaaaagcga tagagaataa tacccctacc gcaaaaggaa
      121 atacactagc cacctttgtt cgtatttcaa ctacttctaa aaagattttt agtgtcatgg
      181 ttcgtactcc ttattttagt tcagaacggt cacccatcgt gtaatgggag tcctccgata
      241 atttgaaggt gtctttgatt ggatcggtca catatacttt atcgtctttt gtcacgaaga
      301 tcgcttccgt cccgttattc aattcgcttt cgacgtattc cagtcctttt ttcacaccca
      361 ttgaaaagac tgctgtagac aacccgtcac catcaattga tttatctgtt atgatcgtga
      421 cacttgcgat atcattgtcg aacggataac ccgtttttgg atcaaacaaa tgatggtatt
      481 ttttgccatc tacttctaaa taacgctcgt aaattccaga ggtaactaat gttttgttcc
      541 gttctttaat agaacctaaa acagatccgc gagccatgtt cggatcttgg atgccgactg
      601 tccagtcttg gttttcgcct cttgggctgt ggcctaagac atagacatta ccgcccaagt
      661 caacgatggc agtggtcact ccttgttttt tcaagacttt cactacttcg tccgtaatgt
      721 aacctttggc gattgcacct aaatcgatga tcattccttt ttcttttaga taaacagttt
      781 tttctttgtc atttagttcg atcttatgat aatccactaa tttcaatgcc tgatcgattt
      841 cttcttggga aggttttctg gcgtcatcaa aaccgattcg ccataattgt gtgattgcac
      901 caatcgccat atcgaatccg ccttgagaat cttggctata ttcgtaggcc cgcttgacta
      961 acgtgtagac gtcatcagaa acttttactg gtttgatccc agcttgctca ttcacttcat
     1021 cgatctcaga acctttttga ttgatcgtga ttttatcacc aagttctttt actcgatcga
     1081 aggcaggttt caatgctgat tctttgcctt catcatacac acggatacga acgtaagtcc
     1141 ctaacaagaa ctgttcatca gaatatggat ctttcaatat cttggtagct gctgaacttt
     1201 cagtggttgt tttatttgct gagttgctgc agccagttag caccataaaa gagatgccca
     1261 gtgtgattaa ggaaaatagt agttttttcc gcatcttttt cctctcttca ttttgaaagg
     1321 tgagacgact gcctaatctg gctatcgtcc caccctccaa cttttattag tatgattatg
     1381 tgattatttt tctgttacga tattatctac ttcgattgtt tgagtgtcgc ctttttcagc
     1441 tgcattcact aattgttgtg cgtacatgat gaatgaatgt gagctgtgtg tagcacctgt
     1501 aacaacttct acattagcag gtgagccatc ttcttcgccc attgctttga cgaattcgtc
     1561 gttcagagta gggatgtaag tttttggttc tgtaccgctt tttgctttca tattttcatt
     1621 atattctgta tcatcttgtt ttgatttacc gtctttgtcg atataatcaa agtttgattc
     1681 agttactttg ccgccagcaa ctgtcatttc gaattgcaca cggtagccgt tagagtagtt
     1741 cttttcttca agcttgtaag tgccgtcttt caaatcagca ccattgtcga tttcgattgt
     1801 atctgtgtta ccagcttgag cagcttggat caattgttgt gcgtagtttt ggaagctttc
     1861 agatgagtga gtagctccag ttacgacttc cacaccgctt gcgctttgtg cttttaagaa
     1921 ttggtcattc aactcaggaa tgtatttttc tgggtttgtg ccagattttg cttccatgtt
     1981 tttgttgtat tctgcatctt ttgtttttga ttcgccgttt tcattgacgt tatcatattt
     2041 tgattcagtg attttgccgt cttttacaac catttcaaag actgcacggt aaccatttga
     2101 ataatttttt tcttctaatt tatatgtgcc gtcttgcaaa tctccgccag ctacaacttt
     2161 tgcagatgat tctgttgtgc tttttgcagt cgaagattgt gctgtagatg attcagaaga
     2221 agagtttgag cttccgttgt catctgcgcc gcaagcgcct aacactagag ctgaaaatgc
     2281 taatacggca aatccagtta caaaacgttt tttcttcatt ttaatacccc accattccat
     2341 ttctagtcca caatttttct ttgtgattca ttatacattc taatcttttt agcctataag
     2401 tcaactattt ttgataaata acccatgtaa tatttcaaac gttttctttc agcaaatttc
     2461 gtgaaaaaac gctttatttt aaaatttgaa aaaaatccaa aaggagttag acaaaagaac
     2521 agaacatcta tataatatat ggagattgtg taataagtat caaactaaaa gaattatcat
     2581 atattcatta tatagatagt ctataaggag cggatgatca atggcaaagc aaaatattgt
     2641 cgttgtagga gcaggatatg ccggtgtttc agctacaaaa tacttggcaa agaaactgaa
     2701 aaaagaagat gtagcaatta ctctgatcga tcgtcattcg taccatacta tgatgaccga
     2761 gttgcatgaa gtagctggcg gacgtgtaga accagaagca atccaatacg atctgcagca
     2821 tttgtttgct cgtcaaaaga atgtacaact tgtcacagac actgtaatcg gtatcgataa
     2881 agaacgaaaa atcgtcaaga ccaaattagg ttcctatcct tttgatcagc tgatccttgg
     2941 aatgggcgga gagccgaatg actttggtac acctggtgtc aaagcacacg gattcaccct
     3001 ttggtcattt gaagattctg taaggattcg ggaacatatt gagaagacag tagcaaaagc
     3061 tgcattggaa ccagatgatg aactgcgtaa agccatgctg actttcgttg tctgtggttc
     3121 tggatttacc ggtattgaaa tgattgggga actgatcgac tggaaaaatc gcctagccaa
     3181 agattataaa ttagatccaa acgaaatcac actgatggtc gtagaggcaa tgccgacgat
     3241 cttgaatatg ttggatcgaa atgatgctgc aaaagctgaa cgctatttgc gtaagcacaa
     3301 cgtggaatta ttattgaatg caccaatcgt agaagtggct gctgatcata tcaaattgaa
     3361 agacggacgc aacattccga ctcacacact gatttggaca gctggtgtca aaggaacttc
     3421 tgacgctgct gactttggat tagaatcagc acgcggacag cggttgatcg ccaatgaata
     3481 tatgcaggca aaaggctacg aagataaaaa catctatatc attggtgatt tagtctttta
     3541 tgaagaattc ccaggaacac cgacaccgca gatcgttcaa gctgctgaac aaacaggaca
     3601 cacagccgca gcgaatatcg tggcttcgat caaaggcgga gaaaagcata aatttaaagg
     3661 aaactatcaa ggatttatgg tttctattgg tgccaaatgg ggggtagcta acctatttga
     3721 taagatccat ttaagcggat ttttagctat cgtaatgaaa catatcgtga atttgaaata
     3781 cttctttgat attcgttctg gttattatat gttccaatat ttaatgcatg agtttttcca
     3841 tatcaaagat gatcgcaacg tgactcgagg acattcttct cgttacggca atgtcttatg
     3901 gagtgtgcca cttcgtattt tctatggttt agtttggttc gttgaagctt ccaaaaaaat
     3961 cattgggaat ggtgaagtgt tcaaaccaag tacttggttt ggtgaaggat catggttcac
     4021 tggaaacagt gtgttccctt ggacatggga atacaatgta acagatgttg ccagtggtgc
     4081 ttctgctgct ggtgatgcaa ccacagctgc cagcggagca ggggcaagtg ccggagaagc
     4141 agcgactcaa gctgcacatt ttggtttgag ttatgcatac ggtgaagaac caatggctgt
     4201 gattgagaag tcaccagact ggttcacaaa gatcatgcaa gtgatcatgc caaacaatga
     4261 tgtagcaatg ttcttccaaa aattcatggt attctttgaa attgccttgg cattagcatt
     4321 gattgctgga ttattcacat ggctatgcag tgcgacaacg attgtattgg tgatcacgtt
     4381 ctgcttatca ggcatgtttt actgggtaaa catttggttc atttttgttg ctttcgcatt
     4441 gatgaacggt tccggacgtg cgattgggtt ggatcgctgg gtaattccat ggctacaacg
     4501 tacactaggt aattggtggt acggtagacc aagttcccag tacggcaaga aataaataga
     4561 taaagaacgt tatttgaatg caagatttca atagaaaggg gctgagacaa accgtcacag
     4621 cctccttttt atgtaaaatt aaaggaataa gatgcccgag ataagcctcc atgagcaaga
     4681 cacaggtgtc acaacctctg ttacaacctc ttgttataaa cggtgttcca gaagtagctt
     4741 cttcgaaaaa taagccgaaa tttcacaaaa acttgaaaag taattttcgt aaattccttc
     4801 ttatttcttg aagttgccca cttctgtcac aacctcttgt tataaacggt gttccacctg
     4861 ctgcttctcg gtaataagcc caaagaatta gaaaacatgg aaagctgttt tctattcttc
     4921 gttcttatta ctcaaagcaa gacacaggtg tcacaacctc ttgtttttat gctaaaattg
     4981 ggtgagttaa tctggaaaaa ggagaacaaa aatgagcctt aaagaatttg ctgaaaaaag
     5041 tctgctcaaa ccttgggatg tttttattat tttgcttttg actgttcttt cttttttacc
     5101 agtcatgatt tttggtcttc agcaaaacac cgcaacagca gaaaaagaag cggtactgcg
     5161 tgtggatggc acagaaatca aaacattccc tttgatggct ggagaagaga gcaacacata
     5221 tacttacaaa gatgaacacg gagattataa cttgatcgag atagatggcg atcgagtacg
     5281 gataaaagaa gcagattgcg atgaccagat ctgcgtacgt cgaggctggg caacgaaaaa
     5341 tggcgaaacc atcgtctgtt tgccacataa attagtgatc gaagtccgtt caacagatgg
     5401 aggtgacgaa gacagtctga tttattagac cttcacacac catgagtaaa ttacaaaaaa
     5461 tgatttatat ttcgctactt gttgcacaag gagtgatcat tggtttattg gaaaatatga
     5521 tcccttatcc tttttctttt gcgcctggtg caaaactagg cttagccaat ctaatcacga
     5581 tcgttgccat ttttaccatg aaaaaacaag acagcttctt actactttgg ctacgactat
     5641 tcctgactac tttgctaggc ggaacgattt ccacattcct ttatagtatg agtggtgctt
     5701 tgcttagtta ttttggaatg ttattggtca agcaattagg accaaaacgt gtcagtatca
     5761 tcggaattag tgcggccggc ggtttcatgc ataatgtcgg acagcttttg atggcttctt
     5821 tcattgcaca atcatggaca gtcatgttgt acttgccagt cctttcagtt ttagggattt
     5881 tatcagggat tgcaatcggg atcgcagcga attatctttt acgacatgtg cagacacttc
     5941 aacgattcca aaaagattat gagcagggaa ccaaacaaaa atggagccca atgtttacca
     6001 aaaaataatc gtcacacaag gagttttttt atgaagctac atccaatgtg ggaaaactat
     6061 cctgctttaa gtaaagaatt gacagaaaca ctaaaacgga tggaagaaac tgtccgcctg
     6121 aaaaacaaac ctgtcgaaca agccatcaaa gaaacgatcc atgccggcgg aaagctgttg
     6181 cgtcctgcct atcagctatt gttctcacaa tttggaccag agcaagatcg agacaaagcc
     6241 atcgctttgg cagcttctat cgaaatgctc catacggcca cactgatcca tgatgatatc
     6301 gttgatgaag cggatctgcg acgtggtcag cctaatatcc gtagtcagtt tggtaatgtc
     6361 gtttctgtct atgcaggaga ctatttattt gtctgctgtt ttaaactatt gtctgattac
     6421 tccacttcct tgaaaagtct ccaattgaat tctcgcagta tggaaaaagt attgaatggc
     6481 gaattagggc agatggatga tcgctataat tatgaacttt ctgtaaaaga atatttggaa
     6541 aatatttcag gtaagacagc tgaactgttc caattaagtt gttctgttgg cgcttttgaa
     6601 agtggaacaa gcgagcgctt cgcaaaaaaa gcaggagata tcggactttc tatcgggatg
     6661 gcctttcaga ttatcgatga tattttagat tatacaaaag agagccagga aatcggcaaa
     6721 cctgtactag aagacatgcg tcaaggggtc tattctttac cgttgatcta ctctttgcaa
     6781 aaaaacaaac caaagctact accttattta gagaagaaag cggcaatgac agaagaagat
     6841 gtcgacgccg tacgcaaaat cgttgaacat acaggaggcg tggaagaagc taggaagtta
     6901 gcggcctctt acacagaaaa ggctttgaag gaaataaaga aattacctgc cacctctctt
     6961 cgaacaaaag agaatctctt ttcccttacc caactgattt tggatcgcaa agactgaata
     7021 gggaaaggtt cgatacgtaa ataaatttct cagaaggaaa tctacgtgtt ttttgacaaa
     7081 ttgaattgta gttcttgtat ttatgataaa aagcaaaaat aaagagcaac gtccgttagc
     7141 gacgttgctc ttttttatct tgaatatttt tttacctttt cgatttctta taactcataa
     7201 aagtgattct taaaccgatt acaagtaata taacgcctgc gagtgttatc caattattcg
     7261 attgacttcc agttttagga agtgctcttt ttgatttgtt tccattgtca ttgcttgttt
     7321 tattagttaa gttaaagttc gtcctgttat ccgaacttgt ttttgagaag gagacacttg
     7381 catgacgtgt atcgcttgtg gtagacgacg tgtctgtact atcgcttgaa gttgatgaat
     7441 ctgagctatc acttgtggta gaggatgtat ctgtactgtc acttgaagct gacgtgtccg
     7501 tgctatcact tgtagtggta gatgaatctg agctatcact tgtggtagag gtagtatctg
     7561 tactgtcact tgaagctgac gtgtccgtgc tatcacttgt tgtggtagat gtatccgtac
     7621 tgtcgcttga agctgacgtg tccgtgctat cacttgtggt agaggtagta tctgtactgt
     7681 cacttgaagc tgatgtgtcc gtgctatcac ttgtggtaga ggtagtatct gtac
//
LOCUS       DANKAZ010000110         7649 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000110 DANKAZ010000000
VERSION     DANKAZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            112..2439
                     /locus_tag="PWY67_002411"
     CDS             112..2439
                     /locus_tag="PWY67_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2058632.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2565..2894
                     /locus_tag="PWY67_002412"
     CDS             2565..2894
                     /locus_tag="PWY67_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2058633.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2934..3938
                     /locus_tag="PWY67_002413"
     CDS             2934..3938
                     /locus_tag="PWY67_002413"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2058634.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3935..4381
                     /locus_tag="PWY67_002414"
     CDS             3935..4381
                     /locus_tag="PWY67_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2058635.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4531..5346
                     /locus_tag="PWY67_002415"
     CDS             4531..5346
                     /locus_tag="PWY67_002415"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2058636.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5552..6724
                     /locus_tag="PWY67_002416"
     CDS             5552..6724
                     /locus_tag="PWY67_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2058637.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6886..7380)
                     /locus_tag="PWY67_002417"
     CDS             complement(6886..7380)
                     /locus_tag="PWY67_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058638.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
BASE COUNT         2596 a         1449 c         1518 g         2086 t
ORIGIN      
        1 aataaaaaga gaaaaaaagc cccaaacttt ctatacatca ataaaataat aaaaattaaa
       61 tttagaataa cgtgtaaagg attatttgat gattttttat tgagaggagg gatgaaaatg
      121 attaagaaaa ctcaaaataa aatccaacgt atatgtcaaa aacattttgt ttctagactc
      181 aaagctgtat ctaaaagaaa atacttacat cccattatga tttcgatcat cttgattttt
      241 actttcttag ggagcttatc aagtatttat gtaaacgttt atacagaaac gtcaaatttt
      301 gctaaccaaa cgacagagga gacaaatagt ccaccctatt ctgaaataca gtccatagaa
      361 agcaccaatc agtctagcaa caacgagaca acctcaacca catcctcgac tcaactgatt
      421 gatccgacag aaaaagaaat cacagcagca aaagcagcag gggagaaagc ttattcagaa
      481 acaggtagaa gccagacatt tagtactgtt gcggccactg cgaatttcaa cggacatacc
      541 ataccagatg gatatacagt gatatatgcc aaagatggag tcattgttgc ggttaacgga
      601 tcgatgagtg cggataaaac tgcgttacaa ggcgtaacca aagcaatcgc ttttattgat
      661 aaccgtccag atatccataa tctgtttatt ggctccacag taaatggtac tactatcaca
      721 ttcccaaaaa gtatctatat gggggtggct accgctggaa atacgatcga ttcagttaca
      781 cccaatgcgg ataaaagtta ttcttttaca ccctcaacat ggggagggct tcctgccgga
      841 ggaggtatga gtactttttc aatcggtata cagttagcaa atgatattcc attcacgggg
      901 tactcgatca aaaatgggca gatcgttgat ggtaagggaa atgtttcaaa ttttccaacc
      961 tatcaagcag caacggttca ggtcaatgcg aatcggacag gaactttttc ttatacagtc
     1021 aatggtggag cagcaaaaat cggaacgtac gggactacat tatctggaat ccatgtgggt
     1081 gacaaagtgg ttatcactcc aacagcagta acgggctatt cttttgcaca aacaaacggt
     1141 tctttcacag ccctagaaag taacgtcgat acggtgacct atacaggcaa acaaacacca
     1201 atcactatta agtatcaaga ctattttgga cagatgattg ctccttctaa aataatgaat
     1261 cttacttacg gaagtgctgc acaagacctg acgactaata cgcctactat caacggatat
     1321 acctttacag ctgtttcagc gatagaaaca aagaaccaat cagcaatgag tgttttagct
     1381 agtttagata caaatggaaa tgtaatcgta aaggacgcaa atggaaaaca gatttctgaa
     1441 gtcatcctat attataagac aaatgcaaca gtatcaatca atgcaaatgg cagtaagtac
     1501 tatgatggtc tttctgttgt accgatcgtt aaatatgcct ttaatgataa aacagaatca
     1561 acatcccttt taaacaatga tctcagtatt gatcaaatca catggaatcc aactgatttc
     1621 aaagcgatga atgaaaaggg agttgaagta actgcgccta ctgaaattgg aacctatacg
     1681 tcttggcaat tgacaaaaag cggactagct aaactagctg ccagaacaaa ctatttattt
     1741 actatcgtgc agacatctga tgtctatacg attaagcaaa tcagcggtgc ggtagagctc
     1801 agtggcagta agacatatga tggaaaagca ggtgtcccgt caatccatgt caagctagca
     1861 gagggtgtca ctagttcgct tactccagta caagtggcac ttagcagtat ggattacaca
     1921 gcagatgcac aatcagcaaa aaatatggtt aatgctggca gctatacaat taaactaacg
     1981 aatagcggaa tagacaaagt caaagcagca aatcccaagt tgtcatttac tgatatagcc
     2041 aacacatcag gaacttatac catctataaa gctaacgcag tgattactgt cgatgacgct
     2101 gcctttaatt atgatgccca aagtcactcg atttcggttg gaaatctcca cgttaccggt
     2161 gttgtcttag gcgaatcatt ggattatacc ctaacagtta acagccggac agatattgga
     2221 tcacaaatag taacgattag tttaggcgaa ggaagtgtaa acaataatta tacgattatc
     2281 gcaacagata ccgctaagtt gacgatcaat ccgactctct tgcttccttc gacaggtggg
     2341 ataggtctac tgccctttgt ctttttagga ctgatcttta ttttttctgg cttcttttat
     2401 tttatccatc gtaagaaaaa agcaggtgaa cagcgatgaa aaaaacaaaa aatgtacgtg
     2461 tggatatatt tgctgttctc ttttttatcc tactggtctt ttttataggt tctccgactg
     2521 tgtcagctag agaatcacct attacattta ctttgcataa tcgaatgttc gaaagcgata
     2581 agatgccaag tatcagtcaa aacgacggaa taaatatccc ggataacttt caaggagtaa
     2641 aagggttgaa cggcgttact ttcgctatat atgatgtctc ggatgaattt tacaagctgc
     2701 ggtcagaagg aagtagtgtg gaagacgcac aaagaaaact agcacaaaaa agtgatagtg
     2761 aaaaaatact ggcagaaaat gtgacaaaaa cagtagacga ggaagagggc attgcttctt
     2821 ttagtgcatc ggacaaagat gagcaaggtc gtgacgctgt ttaccgattt acggaaataa
     2881 agacttcaga ttaagtaaaa gaaaaatcag caccatttgt cgttgtgctt cctgtgacag
     2941 attcatctaa tcataaattg acgaccatcc acttgtatcc aaagagcgaa caaaaaatac
     3001 ctgctgcact cacattaaca aaaacagtag agaataagca gacagatttt gcagatgggg
     3061 ataaagtccc ttatctcatc accgtaacaa ttcctgagaa catcaacgag ataggaacct
     3121 atacgatcaa agatacagct gatcctcagt tatggctaga accagaaacg atcgattttt
     3181 tgattggtgg agacaagatt catacatttc acacacaaaa aataaaacat ggttttgtct
     3241 tatcagattc tggaaaagat cttagttcgt ttgctggaaa aaaattaacg atccgttacc
     3301 aaatgacatt gaaagaaaac agtgaaaaaa cgacatttga taacgatgta cgtttgacga
     3361 ctgacaatca aacagtagaa acacatcttg ccatccaaac aggaggcaaa caatttgtca
     3421 aagtggatcg gcaaacgaaa caaaaactag cagatgctca atttctagtc aaaaaaaatg
     3481 accaatactt agcgcaagaa aacgggataa atcattgggt agccaaagat gattcaagag
     3541 caacgatttt tacttccagt aaagatggaa cattttctgt ccatggattg tcttttggaa
     3601 gttacgaact agtggagatt aaaccgccta aaggatatat attgagtcaa tcaaccattc
     3661 tctttgaagt tgaaaaaggc agttatgatc cgcttcatcc tataccattg gaaatcatca
     3721 acgaaccaaa agcaaatccg acgaataatg gaaaaacgcc agttcagatg cagacaaatg
     3781 gtccatcaaa tgaaaaaaat ggaggaagct ttccaaaaac aagcgaagag atgcttggag
     3841 gtttctcaat tttagggtta ttgctagtcc tttcaacagg gacagcttgg ttttataaaa
     3901 aacaacaaaa aggaaacaat aggagggaaa taaaatgaaa aacatcaaaa caaaaatatg
     3961 gacgttttta ggaactgcta tcatgctgct tcctttcgtt ctcggcctag gaactgcaga
     4021 ggtttctgca gccgtatcac ctacaccaga aaatgttacg gttaatttgc ataaattgaa
     4081 attcacttca gcaccagaaa atcaaataaa caacggaacg gaattgacat ttccgaatag
     4141 cgaaccgttg aacggcgttg aattcaacgt atatgacatc actgctacgt actatccaag
     4201 taaagacaca gcagttccag cagacgctac acccttcgca agtgtaacga cttctggtga
     4261 aggattagct aacctgactc ttccaggtaa atctgatggg aaagatgctg tatacgtctt
     4321 tgtagaaaca ccaaagcccg gtgtcgaaac gtcgccaaat atcgtgttaa gcttacccta
     4381 gaccctgaca aacctttaga cacgattcac ctatatgcta aaaatgtcat caaagaaagc
     4441 aatatcaaaa tccagaaagc tgggaacttt tcaggggtag ataagttagc aggggcgcaa
     4501 ttccagatca aaaattcaga gaatcaattc gtgaccggtt ttgatgaaaa cactggtgtc
     4561 gctctctatg atcctgctga gtcagcgttg acattcacaa ctgatactga aggatccatc
     4621 agtgtcaatg gattaataga cggcaattat actttagttg aaacaaaagc accagatggt
     4681 tatcaattaa ccaatccaca aaaagaaact acttttatag taaaaaacgg aaaattgcaa
     4741 caaacaaccg gtatcatcca tggcgcagat gaagccaatc ttgggaatcc aaacggtgta
     4801 gtcaatcggg aaaatacaac tacggataac acgattactg tagaaaatgt ccaaaactac
     4861 ggtgatttcc acttcatcaa acaagatgtg aacacgcaag aaaaattagc cgatgcagaa
     4921 ttttctattc taagagcaaa agatccaact gttaaattaa tgaaaaaaat agcaaacctt
     4981 gcctcaggag aatacccata tgcttggtca gatgaagtca caggttctga ctgggaagaa
     5041 gtgaaattga tttctgattc agaagggaaa ttttcaatcg aagggctgaa acaaggggca
     5101 tactgccttc aagaaacaaa agcaccagaa ggctacgcat tacctaagaa tgaagctgcg
     5161 tacactgaat ttacagtaga tgatgatcca acaacttctg atgaaacaac gatcgataac
     5221 caacctaaag gaatcttgcc ttcaactggt ggtatgggga tcatcgtatt catcttggta
     5281 ggtactgctt tagttggagg agcagtgatc tatttcaaaa aacgccatca agaaacagaa
     5341 gcttaaacta ttggaaacaa aaatcatacg ataaataaac gatagtgatt caaatgggga
     5401 gaaaggttgt gacaagtggt cggtctcttg gtattcgctc aacaaacggc aaaataggag
     5461 gaactatttt gccgtttgct tcgctaatac tcaggtcaag gcgctgattt cacaacctct
     5521 tcatatacat attagaaaat aggtggacat gatgaataaa agcagaataa gaatactgga
     5581 catcttcatg gcaatcattt tagtcgttgg gatcgggatc ttttcttatc cttttgtaga
     5641 agattcactg aatgattttc ttgcccagca aatgatcatc cattatcaaa agcaagcatc
     5701 gaataagaat tcagaggaaa taaaaaaaca gcaggaaaaa atgacaaaga aaaaccaaca
     5761 gttggcagag caaaacgtat caccagggat aggcagtttc aaccaagcag ttgatgcaaa
     5821 agcattaaaa gatttaccta gtaatgcctt ttttatggaa catatgcttg gagtcatcga
     5881 aatcccaaaa atcaatgtca gcttacctat attcgatcag acaacggaaa tatttttgca
     5941 aaaaggaacg tcattattgg aaggaagttc ttatccgact ggcgggaaaa acacccatgc
     6001 cgttctttct gggcatcgtg gactcccaga agcaaaatta tttacagatt tgcctaagtt
     6061 gaaaaaagga gaccagtttt tcatccagat caatggaaag acattggcgt atcaagtaga
     6121 aaaaatccaa gtggtcttac ctgacgaagt agattctttg ggtatccaaa aaggcagaga
     6181 tctcgtgact ttgcttacct gcactcctta tatggtcaat acacatcgac tgcttgtgac
     6241 ggggcatcgt attccttatc aagcaaaaga ggcaaaaaaa gcgatccaag gaattgatca
     6301 atggaaaaaa tggaaatttt ttatttggtt tatcggtata ctacttggat caatcggctt
     6361 agtttggcta ctcatcgctt atttagattc tttggccatc gctaagcgaa actatccttt
     6421 atctttttat gtaaagaaca caaacggacg accaatcgaa ggaatggtct tttctgtaaa
     6481 aacgctcaac ggcaaacact atattactag agaaaaagtt ccatttgtga aagcttctga
     6541 tgaatacgga ctagtgagat tttctgattt aaaaggcagg aactatcgct tgcaacatga
     6601 ggagctcctg cttaaaatcc atgtaaaaca taaacattcg aaacagtttt caatgaaatt
     6661 gaaaaaagga agatataaac tgcgaaaaga aaaagaggtg tactatttga ttgagaaaga
     6721 atgaatgaag atgtccaaaa aagcagaggg aacagactaa atgaaaaaag agagaagctg
     6781 acgaaatgga cgtgtaaact gctatttttg gtttactatc tggaacgcca gcttctattt
     6841 ttttatgttt cagaagaatt ttattattat agtataactt ttgaattagt ttccataaca
     6901 tttggagcaa ggggttaaac cacgagcttg agcttcgctt aaagtggcag gggtatacgt
     6961 accatttcca catttatgtg tatggtattt tgaaccggtc ggtgtgacat agacagtttg
     7021 ttcattattt tgctcaggag ctgcggcttg tgcctgctga gaagcctgtt cagctgctgc
     7081 ttgctgtcgc gcctgttctg cttgttgctg tgcgattgct tcttgcctcg cctgttctgc
     7141 ttgttgctgt gcgattgctt cttgttgcgc ttgttctgct tgttgtcgtg caatttcttc
     7201 ttgctttttc cgttcttctt ctgcttcttt tgctttgatt gtggaatcaa ttgcagcaag
     7261 acggcttgtc aactcttgtt tgttttcagg tagcgcttgg atagctgctg aagcagttgc
     7321 atagttttct tttgtaggat ttgcttcagc ttgtgacaaa gcagattccg cttctgtcac
     7381 ttttttttct agtgcagctt tttccgcttt ttgtttctct tcttcttttt tcttttcaat
     7441 ttgttctttc gatgaatctg ccgtactttc ggagatcgtg gtactgctgc tggaagcctg
     7501 agctgaactt gtgctttgag tagtagacgt agtagcacca cacccactca acaaacttcc
     7561 tgcgaccaaa aacaacgcaa ctaatttttt cactaaattt cctccttaag taaaaaacaa
     7621 ccgttttcaa taaatgtcct tttcaattg
//
LOCUS       DANKAZ010000111         7532 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000111 DANKAZ010000000
VERSION     DANKAZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(213..890)
                     /gene="nth"
                     /locus_tag="PWY67_002418"
     CDS             complement(213..890)
                     /gene="nth"
                     /locus_tag="PWY67_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL2058639.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(897..1595)
                     /locus_tag="PWY67_002419"
     CDS             complement(897..1595)
                     /locus_tag="PWY67_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2058640.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1863..3047
                     /locus_tag="PWY67_002420"
     CDS             1863..3047
                     /locus_tag="PWY67_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL2058641.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3053..3496)
                     /locus_tag="PWY67_002421"
     CDS             complement(3053..3496)
                     /locus_tag="PWY67_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2058642.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3722..4009)
                     /locus_tag="PWY67_002422"
     CDS             complement(3722..4009)
                     /locus_tag="PWY67_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058643.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4180..5532)
                     /locus_tag="PWY67_002423"
     CDS             complement(4180..5532)
                     /locus_tag="PWY67_002423"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL2058644.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5805..6251)
                     /locus_tag="PWY67_002424"
     CDS             complement(5805..6251)
                     /locus_tag="PWY67_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2058645.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6487..7413)
                     /gene="cysK"
                     /locus_tag="PWY67_002425"
     CDS             complement(6487..7413)
                     /gene="cysK"
                     /locus_tag="PWY67_002425"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL2058646.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2034 a         1543 c         1238 g         2717 t
ORIGIN      
        1 ataggtatat tggaaggcat tattttctac atcgggtatt ttcccaataa aaataacaac
       61 ttttattttt atcgcaataa attgtaataa gttaccatcc tagctactga gtatgtacat
      121 ttactttttc atgcaaaaaa gataaacgaa acagcttttc tccataataa caagataatt
      181 catacattta tttgcaatta aagaaaccac gtctacaggc gtggtttctt ttttagcgcc
      241 ttttctttta gacgcatccg atttttccca tcttgacata ttgatagcaa aggacatacc
      301 tcgcacttgg gatttcttgc cgtgcaatga tatctaccaa aaaaaatcag tgtatgatgt
      361 gtctttaccc ataattcttg tggtactttc cgcatcagtg tttcttctac ttccatgacc
      421 gtagcatcta gtttgcagat cctcaggcgt tttgagacac gttccacgtg ggtatctact
      481 gcgattgctg gtatcccgaa tgcatccccc aatacgacat ttgctgtctt tctgcctaca
      541 ccaggaagag acatcaattc ttctcttgac gttggcactt gcccatcgaa acgttcaatc
      601 agctgctgtg cacacgcttt gatgtttttt gctttatttc gatacaaacc aatcgttttt
      661 attttaagaa tgatttcatc gattgaagct tctgctaacg catcgggtgt cggaaaagag
      721 gcaaacaaat ctggcgttgc tttattcact gatacatctg tcgcctgggc acttaagatc
      781 actgcaatca ataactcaaa tggattattg tgcttgagtt ctccatgtgc ttcgggaaac
      841 attccataca tcgtttctag agcttccatt gttttttgtt tgcttaacat attccgctac
      901 tcctccaacc aatttttcag tgagactttc ggcaattctt cttgttgagc tgactgttct
      961 acttctcttt gaagaagctg ctgttttctt cgtttttggt cttcttccac ttgttgtttt
     1021 gtacgtagat tttttctttc ccacgatagc aaaatgcggt caacgtaatt aaaactataa
     1081 gcctgattta atacggcttc ccgcagtgct agcttcaaaa tttccggctg ataatggtct
     1141 tcttctagcc attgtccgat ccgctggaac tcgatcgaag aaagcgggcg tccaaattct
     1201 tgttcaaata attgatacac tgactggatt tctttttctt gtgttttttc ttcgtatttt
     1261 ttttcttgtg tttttaaata ctcacctaat agatcataga tggggtaaag attatatcga
     1321 tctgcttttt taccgttgac aatcgtcgtt tctatcttga taaatccatt tgttacgaga
     1381 cgatccagta tttgaaaaat ctgatcttgt ttgattccca tatccatagc aatcatctgc
     1441 aaatcaggga aagtatctcc ttcaagctgt gccatatgca actgtagaac aaataaaaat
     1501 tcttcagatt gtagaccgat cctatgataa tttttaagta aaagattaga tatgactgtt
     1561 tgtccagcgc ttaagtaatt ttctaaattc aacatgattt ctcctccgct caatcacagt
     1621 atacgtaaaa aaaacatggg aagcaagaac aggtttcttt tctatttact tcactgatcg
     1681 gtcagattta caatgtcgtc tacttgattt ttgtttgttc atgtttttga tgcgtatctt
     1741 cacttttcgg aataaataaa ttaagtagat ttcgctgttc cttagaatgt tgacaaggaa
     1801 acaaaattac tatacactat ttttgtttcc aagttaacat tattgcataa aggagttttt
     1861 acatgtacag tttcacaaat aaacattcta aagtttttgg atacgtctct gtcttattca
     1921 ctggattagg atttactatc gtcagtcctg tacttccgtt tttaacgctg ccttattctc
     1981 actccataca tcagcaagct ttctatatca ctttgctcat gtctgtttat gctctagctg
     2041 ccttcttatc tgcccccatt ctcggtagct taagtgatca ttttgggcgt catccgatcc
     2101 ttattatcag tttattagga tcaagtatcg gatatttgct ttttggttta ggaaattcga
     2161 tatggatgtt gttccttggt cgtatcattg aaggattgac ggcaggagaa atctccacct
     2221 tatatgctta ctttgccgac atcacggaac ctaatgaaag aaccaaagtt tttggatgga
     2281 tgggtgcttt agtcggtata ggaacaacgc tgggtcctat tatagggggt ctattagctg
     2341 agttggggaa ttctgttcct atatttattg gagcactctt tactttctta aatgccgttt
     2401 atggctacac atttatgcta gaaagccttc caataaaaaa acgttcagta gatttaagtt
     2461 tctcccatgt aagacctttt caccaattac aacaattatt caagatttca tccgttattc
     2521 ctttattgac agcaggtttt gctgtttggt tggcagctgg ctctcttcaa tctatctttt
     2581 ctcaattttc gattgacgcc tttcagtgga aggctgggtt ggttggttta agcttctctt
     2641 tgattggaat cttggatact gtctcgcaat tgttcattat gcctcgattg ctcaaaaaat
     2701 tttcagaaca gcagatcaca agaataggga tgttcagtga aatcctcgcc tatctattca
     2761 tcacattatc tggaattctt ctactgccga ttttgttttt atttgggatt atctgttatg
     2821 gtttcggtga ttctattttt actcctgtat tcaatggcca actatccaat tctgtctcag
     2881 ataaccagca aggtcttgta atgggcggta cccaaagtat acaatctcta tcgcgcgtca
     2941 tcggcccgct aatcgcagga cagctttatg ctgcagcacc atttcttccg acggtattcg
     3001 gatttttgct tatgattttt gcctattatc actacaataa aaaatagtag cttcatttgc
     3061 tacttcgtat aaattctctg tatcgaccga taaaacgaaa tactgtgtca aattcttctt
     3121 ttgacatatt ttcaaataca gtagcatctc gctgtaccca tttttgatgg agttgctcgt
     3181 gtctattcct tattttttgt ccgattggcg tcaattgaaa atagatttct tttttattct
     3241 cttcgctttg ataagtgttt atgatccctc tagctatcag tttttttgtt agcttactga
     3301 tggctcctct agtcatgtaa ctagcattcg ctaattttgt cacgttagga tcagggattt
     3361 tcccgatatg ttcaatcact tctagttcat ttagagtaaa tcctgcaaat tctttttcca
     3421 tttctggttt attacgatga ataatctcat tagctaaatc cagatattct tctattagca
     3481 gttgtctttt atccatttct acaccttctt tttctttcat tatattgttt ttttgtttcc
     3541 tattcaacaa agataaaaat attttgtagg aaaacccaat ctgaaaccaa gaaaaaaagg
     3601 ggttgacaaa gcttgtcata accccttttt ctgattaaaa gatagtaaag agacagtcat
     3661 cggtaattgt gatgattttt tgtcatcact taaggatgag cggccttttc aaaaactgtc
     3721 tttaaagatc atcatcagtt tctagcacat atggctgacc ttcatatgtg tactctgtat
     3781 tcaatgtgcc gtcattttca tctgagtaga tgcgtctact gaaaggaaaa cgatgagaaa
     3841 cattatcttt ggcgtcattt gctggcatcg tgtaatattc ctcttgcgtt tcattcaact
     3901 gagagagtac ttgatctgcc aactcatcaa tattgatcgt taatttcttc gaagcacttt
     3961 tataatcttc taaaaatgca tcttgttgga caaaatattc tttattcatc ttctttctca
     4021 cctttctttt caaaatcaaa taaagctgct tgttctttta tgtaacagct ttatctttgc
     4081 tgcttattgt gacaaaaaat tcgaagaaag acaactaatt gtacttttca caaaaaaaga
     4141 cagagggtcg tgacaaaatt gtgtcacaac cctttttctt tattgtgcag tttcgatttc
     4201 gcctagcgct gcttgtgcaa gtaggttcaa gtaattccat gggcggtcaa aatgtggttg
     4261 gaagaagaaa tctgaaagtg cgagatcttc tacagtcatt ttattttgaa cagctaaaga
     4321 catggtattt gctgattgtg taatgtcgta tttggacatg aactgagcac ctacgatacg
     4381 attcgttcct ttttcataga ctagctccat cattactttt tcagtagttg gcatgaactc
     4441 tggacgataa ttatcttcaa atacagtcgc ttggacttct agatcgttca aagttgcgct
     4501 ttctttggtc acacctgtcg aaccgatcgt ccagccaaat aggtaaagtc ctgaagttcc
     4561 ttgagtacca cgatacgcca ttttatgatc tgtcaagtta tttcccacaa gcaaaccttg
     4621 acgaacggca tttgttgcca aaggaatata gttttgcgtg ccacttggat tgtagttgac
     4681 tactgcagag tctccagctg caaaaatgtc tggattactt gtctgcatgt attcattcac
     4741 tttgattgcc ccgtttggca gtgtatctac ttttccttcg atcagttttg tatttggacg
     4801 gaagcccaca cataaaatga ccatatctgt atcaaatgta tctgttggtg tagctacttg
     4861 tttcactttt ccttcttcat ctgtgataaa ttcagtaaca ttttcgccta gcgcaagagt
     4921 aacaccgcgg tctgtcaatt ctttttctaa gatatctgtg aatggtttat ctagatattt
     4981 attcaagatg cgatctaatc catcaatcaa tgtcacttgt tttccagatt cgacaaaagc
     5041 ttctactaat tcaataccga tatatccacc accaacaatt actacacgtt ttgcttcttt
     5101 cgcttgtgca ataatttcat ttgcttgatt gtagtttttg cataggagta catttttcgc
     5161 atcgattcca ctaatcggtg ggataatagg ccatgaacca gttgtcatca ctaatttatc
     5221 ataagaaacc gtttctgttt ctcctgtttt cagatctttt gccgttactg ttttattttc
     5281 tgtatcgacg tctgttacat cgtgttccat tttaactgtt gctcctaatg aagataattc
     5341 ttcaggattt gagtagaaca agcctgctgg gtcctttact actccgccga cataaagtgc
     5401 gataccgcaa gataagaaag aaatattgtc atttctttca tatactgtca cttctgcttc
     5461 tggatgattt tttaaaatac tttttacagc tgcagtacct gcatgagtac agccaattac
     5521 tactactttc atttatggtc cctccgatta acattttgtc cttcgacaaa actactataa
     5581 cagaaataaa acggatacaa cagtttaatg cttgtgaatt cacaaaggtt tgtttgtagt
     5641 ttcattcctt ttcaagcata agaacccaat atctacaaga ttcttatttt aataatattc
     5701 acttttatat ctaagattgt gaataaaaga caaaaaaata aaaaagctag cgtttttttt
     5761 acacagctag ctttttttag gtataacact tgtagaaact gtttttacat ttgcgccttt
     5821 tttgctgact ctttacaatc cggacagagt ccatacaatt ccaaccgatg ttcattaatc
     5881 tcaaaatcag tcagtctgct tgcagccatt tctatatctt caagccctgg gtaatgaaaa
     5941 tcaacaatct taccgcagtt ttcacaaatt acatgataat gacgctttga actgaaatca
     6001 aatcggctag aagcatcgcc gtacttcatt tcttgaacaa atccaatcga agtaaataag
     6061 cgaaggttat tgtaaacagt cgcaacactc atatttggaa accgatgttc aagtgcttta
     6121 taaatctcat cagccgttgg atgggaatga tgttcaatca aatattctaa aaccgcgtac
     6181 cgttgaggtg tgattcgaat gttctctttt tttaactttt cgataacctc ttcgactaac
     6241 ttattttcca tgtaaacacc ccacgttgtt attctcgtct tatcatacta tttttacatt
     6301 ttaaaatcca cttaacatga aaagaaaatt caattttatt gtattttaat gaaaaaagac
     6361 gtaaaacagt cgcgttcact attctacgtc tctattcaaa taatttttct catcataaat
     6421 ttttgtgtgt tcttttcatc tttaaaagtt ttttacataa acctatctgg agaaaaacca
     6481 agtgccttat atctcttggt aaagggctgt agataaataa cgttcaccat tgtcgggaac
     6541 cacagctaat accttcttgc cttttcctaa ttcttttgcg acttttagcg cggcagcaat
     6601 tgcagcgcca gatgagatgc ctactaaaat cccttctttt cgcccaactt ctctagctgt
     6661 ttccatcgct tcgtctccac taatagaaag gactttatca tacacagaag tatccagtgt
     6721 tttaggaaca aaacctgtac cgattccttg gattttgtga ggtcccgctt ctttcccttc
     6781 taaaatagca gattctgctg gttctacccc gattaattcg attttcggat acacacgttt
     6841 taattctcgg ccagcgccag tgatcgttcc tcctgtaccg atacctgcaa caaacgcgtc
     6901 caagccattt actccaaatg cttgatgtat ctcaggacca gtcgtcttct catgcacaag
     6961 aggatttgct tcattttcaa attgtaatgg taaaaagtag ccgttttctt ttgccaaacg
     7021 ttctgcttct ctgattgatc cagaaatacc atccgcacca ggagtcaaaa tcaactctgc
     7081 gccgtaccct ttcatcaata aacgtctttc gatactcatc gtctcaggca tcacgatgat
     7141 tactttatat ccttttgcaa ctcctaccat cgacaatccg attcctgtat ttccagaagt
     7201 aggttcaatg atagtatcac ctggtttcag tagaccatcg tgttctgctt tttctatcat
     7261 acttaaagca atacgatctt ttacacttcc tcctggatta aaaaactcaa gctttacaaa
     7321 aacatctgct gaatcttctg gtacgatttt atttaatttg acgattggcg ttttgccaat
     7381 caattcggtg actgagttat agatagctgt catcttcaat tcctccttgt ctttgctctt
     7441 cttacttatc ataatacaat ctgttcattt atggcgagtg attcgtcttt gactagcaaa
     7501 aaaaagacaa agatatacca tatatggtaa tt
//
LOCUS       DANKAZ010000112         7459 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000112 DANKAZ010000000
VERSION     DANKAZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..240
                     /locus_tag="PWY67_002426"
     CDS             <1..240
                     /locus_tag="PWY67_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2058647.1"
                     /translation="KDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFI
                     FDKTDKEIGKVLGITRQGATNLKHRLYKKIQKMLV"
     gene            334..525
                     /locus_tag="PWY67_002427"
     CDS             334..525
                     /locus_tag="PWY67_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2058648.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1039..1371
                     /locus_tag="PWY67_002428"
     CDS             1039..1371
                     /locus_tag="PWY67_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2058649.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1667..1822
                     /locus_tag="PWY67_002429"
     CDS             1667..1822
                     /locus_tag="PWY67_002429"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2058650.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2098..3438
                     /locus_tag="PWY67_002430"
                     /pseudo
     CDS             2098..3438
                     /locus_tag="PWY67_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3679..5295)
                     /locus_tag="PWY67_002431"
     CDS             complement(3679..5295)
                     /locus_tag="PWY67_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2058651.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            5413..5631
                     /locus_tag="PWY67_002432"
     CDS             5413..5631
                     /locus_tag="PWY67_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2058652.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5826..6287
                     /locus_tag="PWY67_002433"
     CDS             5826..6287
                     /locus_tag="PWY67_002433"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2058653.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(6480..6719)
                     /locus_tag="PWY67_002434"
     CDS             complement(6480..6719)
                     /locus_tag="PWY67_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2058654.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6743..>7459)
                     /locus_tag="PWY67_002435"
     CDS             complement(6743..>7459)
                     /locus_tag="PWY67_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HDL2058655.1"
                     /translation="ANEAGIKLEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTM
                     VAGEEVVPEKEAKDEKTVANGYFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDY
                     KAKINKDMTAAEYKDYYTTGYKTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNY
                     EKGNRGVRFNFETDDAGAGRFKYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNW
                     PTFYPASLSKHEIAQEMMAH"
BASE COUNT         2478 a         1431 c         1222 g         2328 t
ORIGIN      
        1 aaagatacta taaaatttaa acaaccgatt aatgttttaa atgtgactct ccttgttgaa
       61 gatataaaat tagcaaaaat ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc
      121 gaaaaattta tctttgataa aactgataaa gaaattggaa aggttctggg gattacaaga
      181 cagggtgcga caaatttaaa acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa
      241 tatctgcata ataactaatt ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa
      301 tagattataa gtccatggga agaaggtaga tttatgaatt ctttaaaaga agatttcatt
      361 ttagcaaagg ctggaaatga agaagctgtt gaggcaattc tcaaaagatt tagctctttg
      421 attcataagc aatcctggag aactggaaaa tatgatcaag attgttatca agaatgtatg
      481 cttgcaatat atctagctat ctcgaaattc gaaattaaag aataactcaa ctaagggagt
      541 ctgtgacata agtaaaaaga tagtttcaaa tttcgaacaa taaatttgaa actatcttct
      601 ttataattat aaagtatcga ttttacagcg ttctataatg attcatagaa tctaaaacga
      661 atgattttaa ttatcaacgt tttttaattc gtttttagct taaaaataac ttttgtcaca
      721 gtctcactcc taaaattact ttatttacgt atgaagtggg caggaggtcg aatccagaac
      781 tgggctatgg tacttcaacg aggacttctc ctctagaatt gaaaagcatg cgatttacct
      841 tccgtaacga cggtagattt attcaactat gtgtccaatc aaaatttgtc tgatttcaaa
      901 aatagtcaag agaattttgg ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt
      961 gacaaaccct gatactaagt tgggctatga tacacttttc ttgactatca gttacgttac
     1021 cggaaggaat gaactctatt gagaaaaaca tatgatcgta aatttaaact aaagatttca
     1081 caggatatcc ttgagaaaaa agtaactacc aagaaaattg ctgaagaata caatatctct
     1141 cgtccaacaa tttcccgatg ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct
     1201 ggacaaggaa aaagattacc tgataaagca gatttctaca ttcttgaaca ggaaaataaa
     1261 cgactaaaag aggaagacga gtgttctacg aacacaaaga tcgatacgga tctccaggga
     1321 tcactcaaaa actttacgat gaaggaatcg aaatcaataa acgtgttgta gctgttctta
     1381 tgcgtgaaat gaatttatgc acaagaggat ttcatcgacg caaatcttct catggtagag
     1441 aaaaaacaat caaagaaatg gtaaaaaaat gttgaatcga taatttgaac agaaccaaat
     1501 tgatgctgtt tgggtgatgg atattactta cattccatgt aatgacggtc gcctctattt
     1561 gtccacctat attgacttag caactcgtat tctacgttac tatatggtag attcacacat
     1621 gaaaaaagag attgtcgtcc gtccattaca aatttattaa ggagaattgc ctacggtaat
     1681 tcattccgat agaggtagcc agcatcggtc ttttatttat caagagctgt tgaacgacca
     1741 ccacatcaca cattcagtga gccaaactgg gactcctgtt gacaatgcag taattgaagc
     1801 gtttcatcga tcaatcaaat gagaattaat cgactccaac aaatataaaa gtaaaataga
     1861 gatgaaggtg ttaatccaag attatttaga agactactac ccaaacaaaa gaatccatac
     1921 aaaatttatg atgactcctt gacaatttga agcgaagaaa ataagggatc tataatatct
     1981 agtgttggtg acgccttctg ttgccaatac tttttctttt ttctaataaa cgcaaacaga
     2041 caccttaaaa tcctttagaa taaagtcgac gaaaaccatt caaaggagtg tttcaagatg
     2101 tcctattcag agtttaccaa agagttacta gatattctag acctaaatct tatctttcat
     2161 gaagatgcct ttcgcaaaga gcgaatcaat gacgaaacat gttttgtttt tgatggcaca
     2221 ttgacctatc aaccggagga atgctttcat cgccactatc aaaatgaaca aacgatcatt
     2281 aaatagggct ggaaaaaagt atccatctta ttgaatgatg tcagtaacta caagaccatt
     2341 ctgcggatta acaaacagcg cttcaaatgt aaacactgtg gaaagacttt tttagcagaa
     2401 gattcagtta gtgatcgtcg ctgctccatt gctcgcagag tcaaacaagc cattcttgag
     2461 ctattaagcg aacctctttc tatgtctttg atcgcacgta tgaagcacat ttctccgacc
     2521 actgtcattc gaatacttcg cagccttcgg cctaaaacca tttctttgag ccagctgtta
     2581 ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc
     2641 tttgtcatga tggacggcaa gacccatcaa ctgattgata ttgtggaaaa tcggcaactc
     2701 aatcctttac gcgactattt cttgcgctac cctataaaag tccgtgaaca agtgcgtcta
     2761 gtggtttcag atttctatac accctatcgc acattggtga aagagtgttt tccgaatgcc
     2821 cgaatcgtcg ccgatcgctt tcatattagc caacacattg gtcgggcttt taccaaccac
     2881 cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa
     2941 tactggaaac tacttcaaaa gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt
     3001 ccgtcatttc gagatcatct gacagaagca gagatagtcg atcggctatt atcttatgat
     3061 gattccttaa agcgaggcta cgaagtctac caggattttc tttccgttat cagaagacaa
     3121 gatgttcctg aattcgtcgc gctcctaaaa gaagactaca aggagttgcc agagcactac
     3181 caaccagtgt tcacgacctt caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt
     3241 ccctattcca acggccctat agaatgtttg aataaccata tcaaagtact aaaacgaatt
     3301 gcctatggct atagaaactt tcaaaactac cgagaacgaa tctttttata tcgaggaaaa
     3361 tattttaaga aaactaagaa tactacccaa ctgactaaag ccagaacaac aacacggcta
     3421 gacaagatag ctgtctagcc gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa
     3481 cactcattga cgaagagcca aaataagtaa tctaaccgac tgttaactaa accattgcag
     3541 gtcccgttaa agatacttgt gcctgttgtc accgcttctt tttcttgtcc acaagcgctt
     3601 aacacactta ataaagcccc tgctattatt aataataata tctttttctt attaaaaacc
     3661 tcctcatcta tttccagatt agtaaatgta gtaatctgtg ctttcttttt tctcaatact
     3721 aataactaac ttatggggta aacaaatact cgtttgaccg ggttttgaga tccatccagt
     3781 tctaaccgcg atttgatcag gactattatc ttctttaact cgaattcttc catctttaat
     3841 ctcaacaata ttatattgtc ccttcgcagg atgataattc acgagtttat gattgatctt
     3901 atccaaatcg aagcgatcca tttccttgcc atcaattcga acgatggcat atttgatagc
     3961 atcttcagtt gtttcattct ccttttctat agagaataca aataagggaa taaaagaggt
     4021 aataatcaaa atgccaataa ttatgacatc gcctatcttg acaccagctt ttcttaaaaa
     4081 tttcattaac gatatctggc taaacatatt agactttttc catggaaatg gtgaaatttt
     4141 tcggaaatta tctgcattaa gagaatcagt taaacaaccc tcaagttgtt ccttaataca
     4201 ttgttgatcc atcttcatac caataaaaac tagtttatta atgcgttcac caaacaacgg
     4261 atgccaggct tccttcaatt cgggattttt ttgtataatc atttgacact caattttagg
     4321 taaactagct acccaataat ttaatgattc aatactttta tagctacccg atgtttcaaa
     4381 gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt
     4441 gccaggaaaa tttttatccg taagatgggc aaatttttgt gcgtcaagga gtaaccgatt
     4501 tcgatagata aaacttgaga taccatattc ttctgtttca ggaatatgat tttgatagcc
     4561 aagctctaac tctttgaacc agcccactga ataggaagtc tcatctatat taaaaagatt
     4621 tgtatttaga attttcgata agtctacttc acctttaatt gtcctaatta ttttggcatt
     4681 ggactgtaac ttagcaatta gaccgttaat tttgtaaatc gtcttggttg acactaagtc
     4741 actattattt aaaagtaata tgttacaaca ctcgaattga tcaattaaca agccacgaat
     4801 atcctgttct tcttcaacta cattaccttc agcttttctt tcctgaatct tttcacccgt
     4861 ttcgaaatta tcccaaaaac gttttacatc cactactata ataatggcac caatctctaa
     4921 tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat ataattgctt gaataattgg
     4981 aattggtttg ctgattcctg aagcttcaat aacaatttga ttcaagtcag aaaattgcac
     5041 taattgatct agttcaacaa tcaaatcttc gcgtaatgta caacaaatgc atccattcgg
     5101 catcgaaaca agtttttctc cagttctttt aaaacctttc ttgtcgacta ataccccatc
     5161 aacatttact tcccctatgt catttacaat gacagcaatt tcctttccgt gatcaccgta
     5221 cagaatatga tttagaagta tcgtttttcc tgaaccaaga tagccagtta agattgtaat
     5281 aggtattttt ttcataggga acctctttct ataccttgaa tttacacttt aagtgcaaca
     5341 cataaaaatg agtcataatc gatatccaac tagtataagt ttgtcgagaa cactcctaga
     5401 aggaaaataa ttatgagcca gtatcgtcat cttactatac tcgaacgaga aagaatcttt
     5461 ctgcttcacg aagaaggatt ttctattagt ataattggca acgacattca gcatagcccg
     5521 tttactattt tacatgaact gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg
     5581 aaaaaaatta tgcttatcga agaaaaaaat gcggacgtta tcacattcta aatgatccgt
     5641 gagtttggtc aattgttcgt catttatttg tcgattttca gtggtcgcca gaggaaatct
     5701 cgaatcgttt gaagcatgaa catatggact tttcaattag ttttatcacg atctaacgtg
     5761 caatttgcaa tgatcttttc gatacagaac ccctctcaca cggcaataaa ggcctgatcc
     5821 gttcattgca ccatcgagga aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa
     5881 agatttgcct tactcattcg atccatggcc gtccaaaatc cgcagatgat agaagtgatt
     5941 ttggtcattg gaaagccgat attgtagctg gcaaaactgg tggtacttgt ttagtgactt
     6001 tgacagacag aaaatcatgc ttccttttag caggaaagaa ggtccccaga aagaaagccg
     6061 cttatgtgag cgagaaaatg attgaacttt taagcagtat tccaaatgag gcggtcaaaa
     6121 cgatcactcc agatagagga aaagaattct ccaatcattt cgcagttaca gaagcattga
     6181 ataatatgcc cttttatttt ccagatcctc atgcccttgg caacaatgaa caaattaaaa
     6241 tacgaataga ctcattcgag aatacttgcc aaaatcgaaa gagatagcta cggtagataa
     6301 ctcaacgata aatcagatga ttgaaaaact gaataacaga cctagaaagt gtttagattg
     6361 gagaacacct tatgaagtct tctttgataa agtgttgcgc ttgatttgac aattcaagac
     6421 ataataaaaa gcagatcaaa tccaaataag ggaaaaatga tctactctgg tattatcaat
     6481 taattggcca agctattatg ccgttttgct aaacgtctac ttcttccagt attgaccttg
     6541 ataaaacaac aatcaggatc ccgttttaat actctatgaa ctgtgtcaat actaacatgt
     6601 aaagttgcag caatatcagc taatgtactc ccatttaatc tcatttgctt tgcaaatcga
     6661 taacgccgat aagcatcaat attcttggaa tccatagaca ataatacttt tcttaccatg
     6721 tatttcctcc aaaaattgaa ttttaatgag ccatcatttc ttgagcaatt tcatgtttag
     6781 acaatgaagc tggatagaaa gtaggccaat tatgcaattc attatagagt ttttcttgac
     6841 tttctccacc aaagaaaata tggaaatgct ctgccttact tggtgcaatt ccatgatcac
     6901 taaattgaat atatttaaaa cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa
     6961 caccacggtt tcctttttca taattcaaaa ttttgtatcc aacatatttg tatgtgtatt
     7021 gatgatgttc accgttgact acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa
     7081 tatctgtctt atagccagta gtataataat ctttgtactc ggcagctgtc atgtctttat
     7141 tgatttttgc tttgtaatcg aaaacttcat ccaaaattcc atcttttaac aacggataaa
     7201 cagattgcca ttcgcctgta taatcactca attcgcgatc cttcacatcg gcatctttaa
     7261 agtacccatt agcaactgtc ttttcatcct tcgcttcttt ttctggaact acctcttcac
     7321 cagcaaccat agttgttttt tctaacgctt ttagattgtc ttccattact gaaacataat
     7381 cttcaccatt atccatttgt tcttttgtaa gactttcaag aggattcagt acttccaatt
     7441 ttatcccagc ttcgttggc
//
LOCUS       DANKAZ010000113         6866 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000113 DANKAZ010000000
VERSION     DANKAZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(1..1082)
                     /locus_tag="PWY67_002436"
                     /pseudo
     CDS             complement(1..1082)
                     /locus_tag="PWY67_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1325..1780
                     /gene="tnpA"
                     /locus_tag="PWY67_002437"
     CDS             1325..1780
                     /gene="tnpA"
                     /locus_tag="PWY67_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL2058656.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2045..2143
                     /locus_tag="PWY67_002438"
                     /pseudo
     CDS             2045..2143
                     /locus_tag="PWY67_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2826..3644)
                     /locus_tag="PWY67_002439"
     CDS             complement(2826..3644)
                     /locus_tag="PWY67_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2058657.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3805..4494
                     /locus_tag="PWY67_002440"
     CDS             3805..4494
                     /locus_tag="PWY67_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2058658.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4508..6010
                     /locus_tag="PWY67_002441"
     CDS             4508..6010
                     /locus_tag="PWY67_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2058659.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6023..6499
                     /locus_tag="PWY67_002442"
     CDS             6023..6499
                     /locus_tag="PWY67_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2058660.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6596..6706)
                     /locus_tag="PWY67_002443"
                     /pseudo
     CDS             complement(6596..6706)
                     /locus_tag="PWY67_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2233 a         1076 c         1248 g         2309 t
ORIGIN      
        1 accttccatt accgctaatg cattgggctt ttcagagata cgataggata aaatctcact
       61 attgaacata tccataaaag gatctagata caatttcttt tgacggataa tgcctttggc
      121 atccacttca tggtatttaa attcagttgt atccgtcgtg attttctgat ggcagatact
      181 ggtgtagaaa cgacgatgga tccgattttt ggctactgct ccgacttgtc ctcgataaga
      241 attgtagcgc cgtgatttac gtgtgtatgt cgttactttt attcctagct tcttgatcag
      301 gcgctgaact ttctttttat tgatagagaa accgcggttt cttagttcat tggtcatccg
      361 taaacagcca taatccttgt gcttctcccg aactttaagc atttccgctt caatctcttg
      421 atctggattt ttacggtcca atcgtttttg ccagtacata aagattgcct tagggaactt
      481 taaagtttct agaagctcaa ctaatttaaa cggtcctcgg aggcttgcga tgattcgtgc
      541 gattgcttca ttcgtcgttg ttgggcttcc tgttttcgca gcttcctcaa ttcttttaaa
      601 aaggcatttt caatttgaag ggaacggact cgtttctcca actcctcgat gcgttcacgt
      661 tctttagacg ttgctttagt tgtagatttt ttcttatttt tgggttcatt tttcttagac
      721 aagataggtg gacatccttt cgtttttgag agtccatcga tcccaccctc acggaattga
      781 cgcatccaac tagcgatcaa attagggttg ttcatgttaa gtgtgttggc aacttcacga
      841 taggacagct cacttgttag atataactct atcgcatcca gcttaaattg aacagaatat
      901 tttttctttt gtcgctttct tactagaccc tcttcaccaa attcttgata ggcattgatc
      961 cagcgcttca cttgtgatgg agccttaatg gaatatttct ttgccagata atcacttccg
     1021 cctttaccat ccaagtaatc atgaacgatc tttaatttaa gttcaaaagt atacttagcc
     1081 ataaaaaata ccgacctcca aaagttagat tttttggtct aacttttggg ggtcggtaca
     1141 aaacttaaaa gttttagacc cttttatttt cttaatgact tcagtcagtt ttgtgcgtaa
     1201 agcgtgcgaa acaagaaaac cgcgcgtttc tacgcgtgga agaagtatct ttagcgataa
     1261 aaactccttt cgatatacta gtgagtggct accaaccgct catcagatcg aaaggagttt
     1321 tttcatgtca aatgacgata aaagtttagc atatactcgt tggaattgta agtatcatat
     1381 tatttttaca ccaaaatatc aacgaaaagt aatctatggg caattacgaa aagatcttgg
     1441 aaaaatactt aggaagttgt gcgaaatgaa agatgtggaa atcatagaag cacatgcaat
     1501 gctagatcat attcacatgc ttgttcgagt accaccaaaa atatgtatat ccagttttat
     1561 ggggtatctg aaaggtcgta gtgcggtact aatccacgag cagcacgcaa atctcaaata
     1621 taaatacgga aacaaatcct tttggtcgaa gagatactat gtgagtacag tcggattgaa
     1681 tcaaaagaca aatgaaaaat acatccgtga acaagaagct gatgatcgca ttcgatatat
     1741 tataagtaaa agagagtacg tagaaccatt taaaaaataa agagaagtaa gtgagtgaag
     1801 atgagcagtt ggcggtcttt taaaaatccc cttttaaggg gcagcgagtg tcgacccctt
     1861 ctagggggat taaaaataca cccgtcctca cgggtgtatg ggttactgat agacttgatt
     1921 ttaactggtg tcagaaactt atgtccagaa agggtgatga aattttggca atttacggct
     1981 attcaagaat tagcacaagc aaccaagatt acaaaacaca aattcaaaca ttagaaaatg
     2041 ctggcgcaga aataatcttc ctagaaaatc actggattat cgaacacctt tggaagtatt
     2101 tttgagttac gtaagtattg atgatctgtc taacttaatt tgacaattaa gagtatattt
     2161 acttcagatg gtaaggcaac aaaataaaat acaataggca gatgaactaa taaataaaat
     2221 ctgtattaaa tacagataaa ttaatataaa aatcaaaagg atcataaact tttaacaagt
     2281 gatgaaatgg gtggaaattg attcttatag acagtttgtc tcataacatt gaagtatttt
     2341 gcaattttag cattgaaaca cttttttaat cttcaacgtt gttcttataa acaagatgat
     2401 tgggaatctt ttgaattaac actgtatagc ttagaacacc ctttatagaa aacattcgct
     2461 tagcaatctt aataattcct tgtgcccgat gtcgtttaga ttcagtaggt tcctgctcat
     2521 cgatcatgga aaaaatttga aggatcagat ccttaataaa tttatttaag agaggatgat
     2581 ccattgcttc atccatactc gataaactag tgataatgag ctacacgttt ttctttcaag
     2641 ttgatcaata gcttccacga taaaaatatc tttagataaa atcaagaacc gtaggaacta
     2701 cagggatatt tggtcaataa gagccaccgc tattagtaaa aaataagcta tcaagtatgc
     2761 tctcatccca agaattgcct acttcaatcg ttaaaaaccg ttaggtttag ctaagtgggg
     2821 gtagttcact caatactttt atcaataata gagcgcaaaa ctttttctct taattcaact
     2881 gaatcaatat tattgttttg ttcataacca tgaactaata gatcacaaat ataaagttgt
     2941 gatatttttc cagctaaaga tccaccatta agaaattctt caatggctgt ttgtaaaact
     3001 aagtctgcag actttcctat aggtgaatga atatagttag ttatagcaat aatttttgcg
     3061 ccattttttt tagctatttt caatgaatca taagtatctt tcgttttccc agataaagaa
     3121 aaaatgatta ctaaatcatt aactgttaat aaagaagcga cttgggcttg aaaatgaggg
     3181 tctagtacag cttttgcttg aactcctacg cgaagaaaca tattttctaa atcaagacta
     3241 gtatttccac tagatcccac tccaaatata tatatgtttt ttgattgagt gatcatttta
     3301 atggcttcat tcaatttttc ttcgtctaaa agcctaatag tggaatagag agcttctgtt
     3361 aaactattag caatttcatc atagtacttt acactagtcg gatgttcttt tttttgacta
     3421 aagtcttcct ttgcaatttc aatttttaaa tcagaaaaac cagaaaaacc aattttttga
     3481 caaaagcgaa taatagtagc atcgccaact tttgtgtttt tcttaatatc actcatcgta
     3541 ctataaataa tagttttccc agaatttaaa ataaaatcag cgactttttt ttcagacttc
     3601 gttaaaaatg gataattttc tttaattaaa tagatataac tcatacaaac tcctctacaa
     3661 tttcaacttt tctccattat actccaaaat aataaaaaaa actcctaaat actccatttt
     3721 tatctttact tttatgaaag cggatgctat attgtttttg gagttgttat ccaaaaataa
     3781 taaaaaattg gagtgtttag gataatggat aaaaagaggt ttttagatct tatttcaaaa
     3841 ggtttgattg tttcctgtca agcattacct ggtgaaccat tttacaaaga ggaagaaagt
     3901 ttaatgcctt attttgcttt ggctgctcaa agggctggag cagttggtat acgtgcaaat
     3961 tctgttcgag atattattga aattagaaag tgtgtatcat taccaatgat tggaattatt
     4021 aaaagagatt atccaccaga atctccattc attactgcca ctatgagaga agtagatgaa
     4081 ttagtagaaa ccggtgttga ggtaatcgct cttgattgca ctcttagaaa tcgacatgat
     4141 ggactatcta taaattcttt tattactaaa ataaaagaaa aatatcctca tcagttattt
     4201 atggcagata tttctacatt tgaagaaggc aaaaatgcat ataaagcagg aatagatttt
     4261 gtaggaacta ctttaagtgg atatactgag gaaagtaaaa aacaagatgg gccagatata
     4321 gatttgatta aaaaattggt agaagaaggg atttgtgtaa ttgctgaggg aaggattcat
     4381 actccagaac aagcaaaaga gataaaaaaa ataggagttg caggtattgt tgtgggtggt
     4441 gctattactc gaccacagga aattgcatca agatttgtta aagcattaag ttagggggag
     4501 aaataaaatg tttaaaaaat tttcacaatt aggacgcgct ttcatgttac caattgctat
     4561 attaccagta gccggattac tattgggact gggaggagcg ttaacaaatg agagtgctat
     4621 aaatgcttat ccatttttag atcaaccgtg gttgcataca attttaagta ttatgagtta
     4681 tgctggaaat gcagtttttg caaacctagc actaattttt gcaattggta ttgctgtagg
     4741 acttgcaaat ggagataaag gaacagctgg tttagctggt ggagtagctt atctagtata
     4801 tactgcaact attagtgggt ttcttgaact tttttcagca aaagatgcaa cactagatac
     4861 aggtgtagtg ggatcaattg ttgttggtgg tactgttgca tttttacaca atcgttatag
     4921 aaaaattgaa ttaccacaat tcttaggttt ttttggagga tcaagattta ttccgattgt
     4981 ttctagttta gctgcaatca ttattggttc tttcttttat ttaatttggc caccaattca
     5041 aaatggttta gtagttgctg gtgaacaaat tgcccaaatg ggtagtttag gaactttttt
     5101 atatggtttt ttattgcgtt taacaggtgc tgttggacta caccacacca tttacccttt
     5161 gttctggtat acatctttag gtggtaccga aactgtagct ggatcaacaa ttgctggagc
     5221 ccaaaatatt ttctttgccc aactagctga tccaaaccac actggtctgt ttacttatgg
     5281 tactagattt tttgcaggaa gatttgcaac aatgatgttt ggattaccag cagcatgtta
     5341 tgcaatgtat cgtgcaattc ctaagaaaaa tcgtaaaaag aatggtgggc tttattttag
     5401 cggggccctt acatcatttt taactggaat tactgaacca gttgaatata tgtttttatt
     5461 tgttgctcct tggctttatg tgattcatgc attcttagat ggattatctt tttattttgc
     5521 tgatatctta aatattcgtg tagggaattc cttttcaggt ggtttgatcg actttttatt
     5581 attcggtgta cttcaaggga atgacaaaac taattggtta aaattaattc cttttggtat
     5641 tgcatgggca tttatatatt tctttgtttt tacattcttt atcaaaaaat ttaaggtagc
     5701 tattcctgga atggaagaag aatcagaagt tcattctgaa aaagtaataa acaaaaatac
     5761 atcattacat gaagagtctc ttatcattat tgaagctctt ggtggaaaag aaaatattga
     5821 gtctgttgaa gcttgtgcaa ctcgtttgcg tgttgctgtt acgaatgggg agattattca
     5881 aaaacaaaca ataaaagaca ctggtgcaac tgctgtcttc gaagtgaagg gtggcatcca
     5941 agctgttttt ggagggaaag ctgacttatt aagtcaagaa atcaatcaaa tattaggaaa
     6001 agataattag gaggttttta atatgtttga aattttcaaa aagaaaaaac aagaaacttt
     6061 ttatgcacct tgttccggaa aaatcatttc catcacagaa gtaaatgatc ctgtcttttc
     6121 acaaaaatta ctaggtgatg gttttgccgt catacctgat ggttcaaaaa tttatgctcc
     6181 attggctggt cgagtaacaa atatatttcc tactaaacat gctattaata taaaaagcaa
     6241 ctcaggcatt gattatttga ttcatattgg aattgataca gttgaattac aaggagagcc
     6301 tttttcgatt tgtgttcaag aaaatgatga ggttactcct gaaacctcgt tggttgatgt
     6361 ggattttgac aaaataataa aatctggtaa agatacttct gttatagttg tttttccgga
     6421 gaaaaatcag ttcaatgatt taataataga taatggtcga attgctcatg ggaattcatg
     6481 tggaaaaatc actttttaaa aaagtgtata taaaaagcta taaaataagc taaataggat
     6541 atttgtcaaa tgaagttggt gaacagaaag aactgttttc tttctgttca tttttttatt
     6601 ttattagtaa tgattcccca tgccaaatca tgatccgtaa tttaaagtta taaaagcttc
     6661 gaaatccatt ggcatttcgt tggaatacct tgatatgatt attcaaatat aaaatataga
     6721 gagaagagag agagggaaga tatttataaa gggcaaagtt atgtttactc aacttaggaa
     6781 atagaaatag atcaatttat attctcttgt caatcgaata tgaaccttag cactatatac
     6841 caaatatagt aaaatgctga aagatg
//
LOCUS       DANKAZ010000114         6505 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000114 DANKAZ010000000
VERSION     DANKAZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(110..565)
                     /locus_tag="PWY67_002444"
     CDS             complement(110..565)
                     /locus_tag="PWY67_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058661.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="PWY67_002445"
     CDS             complement(719..1927)
                     /locus_tag="PWY67_002445"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL2058662.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="PWY67_002446"
     CDS             complement(2039..2371)
                     /locus_tag="PWY67_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2058663.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="PWY67_002447"
     CDS             2519..3394
                     /locus_tag="PWY67_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2058664.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="PWY67_002448"
     CDS             complement(3476..4057)
                     /locus_tag="PWY67_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL2058665.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="PWY67_002449"
     CDS             complement(4063..5322)
                     /locus_tag="PWY67_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2058666.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="PWY67_002450"
     CDS             complement(5324..6370)
                     /locus_tag="PWY67_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058667.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1820 a         1341 c         1105 g         2239 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acata
//
LOCUS       DANKAZ010000115         6359 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000115 DANKAZ010000000
VERSION     DANKAZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(113..301)
                     /locus_tag="PWY67_002451"
     CDS             complement(113..301)
                     /locus_tag="PWY67_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL2058668.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            571..933
                     /locus_tag="PWY67_002452"
     CDS             571..933
                     /locus_tag="PWY67_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2058669.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            985..2667
                     /locus_tag="PWY67_002453"
     CDS             985..2667
                     /locus_tag="PWY67_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL2058670.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2784..4820
                     /gene="recG"
                     /locus_tag="PWY67_002454"
     CDS             2784..4820
                     /gene="recG"
                     /locus_tag="PWY67_002454"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL2058671.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4869..5870
                     /gene="plsX"
                     /locus_tag="PWY67_002455"
     CDS             4869..5870
                     /gene="plsX"
                     /locus_tag="PWY67_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL2058672.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5992..6234
                     /gene="acpP"
                     /locus_tag="PWY67_002456"
     CDS             5992..6234
                     /gene="acpP"
                     /locus_tag="PWY67_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2058673.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2125 a         1104 c         1441 g         1689 t
ORIGIN      
        1 tcgtataaca aaaataaacg caaaaaaagg gtaggaatca aaagtcccac ccttaaaaat
       61 ttttattaaa taaaaaaccg ttcactatct gaacggtcca agtatcattc aattaaacgc
      121 gttcaacttt tcctgatttc aaagcacgag ttgacaccca aactttttta ggttttccgt
      181 cgaccataac gcgaactttt tgtaagttag gtttaacagt acgttttgtt gagttcatag
      241 cgtgtgaacg gttgtttcca ctttttgtct tgcgacctgt aaagtaacaa acttttgcca
      301 tggtaatttc ctcctttgcc ttgatttgga gcaatagaat aattcagctc ataccagact
      361 aacatatcat atagaatggg aatttgcaag tacattttca tagatacaat tgacaattga
      421 gaagaataat tgtaaattaa acaaggttat acctgaaatt ttgcaaggtt tttgagtact
      481 ttgggtaact gccttttatt tcaagttgcg ctatgataaa atatgataga agaaaaatgg
      541 gagtttttta cccgttaagg aggctcattc atggctgtaa aaattcaaac gtcagctgga
      601 accattgaaa ttaccaatga tgtaattgca acagttgttg gtggcgccgc gacagatgtg
      661 ttcggtgttg taggaatggc cagcaaaaat caaattaaag ataatattac tgagattttg
      721 cgtaaagaaa attactcaag aggagtagtt gtccgccaag aagataatgg tattgctgta
      781 gatgtttata cgattatcag ctatggtacg aaaatctctg aagtttcccg caacgtccaa
      841 gaaaaagtga aatacaatct agaggcatta ttaggcgtaa ctgccaattc agttaatgtc
      901 tttgtacaag gtgttcgtgt actgccagac taggcaggag actggctggt aactcagcag
      961 tcgatctgaa ggaggattat ctaggtgagt gtaacagaaa taagtgcaag ccagttccag
     1021 gaaatggtcc aggcaggtgc gaatcgtctg caagtaaatg cagaatacgt caattcatta
     1081 aatgtattcc ctgtgccaga tggcgatacg ggaacaaata tgaatctatc tatgactagt
     1141 ggagctaagg cagtagtaga ttcaacctca gaaaaagtag gagaattagc agcaatgctt
     1201 tcaaaaggat tgctgatggg tgcgcgcgga aattccggcg ttattttgtc tcaattgttt
     1261 cgtggatttt ctaagcaaat tccagatgtt gtctcattaa atgcttctga tcttgctgca
     1321 gccttcactc atggagttga aactgcgtat aaagcagtaa tgaaaccagt agaaggaacg
     1381 attttgacag tagctagaga agcagcgaaa gctggagaaa agaaagcaaa aagtactgat
     1441 gatgtcgttg aagtaatgac agctgttgta aaaggagcaa agcgtgcttt agctaaaact
     1501 ccagacttgc ttccagtcct aaaagaagtc ggtgtcgtag acagtggagg gcaaggactg
     1561 ctgtttgttt atgagggctt tttaagtgca ttgaatggag aattccaagc ggatgaaaca
     1621 tatgaacctt ctccagcaga aatggatgat atggtcaatg cagaacatca tcgcagtgtc
     1681 caaggtcaaa tggcgacaga agatatccat tttggttact gtacagaaat catggtaaaa
     1741 atcggagaag gacctacagt cgatagtaca ttcgattacg aagaattccg taactattta
     1801 gatggtatag gagattcttt acttgtggtc aatgatgacg agatcatcaa agtccacgtg
     1861 catacagaaa acccaggaga agtcttgaat tatggacaaa aattcggttc attagtcaaa
     1921 atcaaagtcg acaacatgcg cttgcagcat gagacgattt tggaacacga tgaagaagtg
     1981 aacgagtttg cgaaaaaaga aataccacgt atcccgtatg gtatcgtagc gatcgctgct
     2041 ggtaaaggcg tccaagagct gtttgaaagt ttaggcgcta attatgttat cagtggtgga
     2101 caaacaatga atccaagtac ggaagacatt gttaaagcga ttcaagaagt aaacgcagat
     2161 aaagtgattg tcttgccaaa caataagaac atcttcatgg cagctgatca agcagcagaa
     2221 gtagcagata tcccagtagc agttgttcct tcaagaacga tttcccaagg attgacagct
     2281 atgctttcat tcaatgatca agcttcttta gaagaaaaca aagaaacgat gacagaaatg
     2341 ttgtctagcg ttgtgagcgg tcaagtcact cacgcgatca gagatacctc aattgacggt
     2401 gtagagatca aagaaggaga ctttttgggc atgatcgatg gaaaaatcct gatctctaac
     2461 ccagatatca cagcaacagc gttaagtaca ctagaacaaa tgatcaatga ggacacagag
     2521 atcgtaacga tcttggcagg tgatgaagga acagttgaac aagcaaatga atttgctgag
     2581 aaactaacag aaaaacatcc tgatttggaa atcgagatcc accaaggaga tcagcctgtc
     2641 tatccttatc tattttctgc tgaataattt ctgtgtcaga atatctttct aacacagtat
     2701 tcattcataa aaggccttcg gttagtgatc aggtatgatc gctgaccaag tccttttttt
     2761 gttaaaggag ggaggaaaaa tttatgcgtt ctttagatga tccaatcact atgctggcag
     2821 gagtcggaga aaaaagagca gctagtcttg ccagcttggg catccaaacg attgaagact
     2881 tactgactta ttatcctttt cgctacgaag atattcagga aagaaatcta aacgagatcc
     2941 aggatcaaga aaaggttact ttgaaaggaa tcgtagtctc gcctcctgtg atgaatcgat
     3001 ttggttataa aaaaagccga ttgcaatttc gtatgatgca agaacatgat gtgttcaacg
     3061 tttctttttt taatcaacct tatctaaaag aaaaagtcat tttatcggag gaaatcgccg
     3121 tatacggtaa atgggatgca aagcgtaaag ccttgaacgg gatgaagatt ttaggttctc
     3181 aaacaatgga tgacttttcg ccgatatatc acgtcaacaa atcaattcgt caaacgacat
     3241 tagttgatct aatcaaaaaa gggtttcaag aatacggcac attgattgaa gaaaatctgc
     3301 caaacaacct tgtggagaaa tatcgactgc ttgaccgagc ttctgctgta agaagtatgc
     3361 attttccaaa agaccacgaa gaaaaccatc aagccaaacg acgtgtcgtt tttgaagagt
     3421 tctttttatt tcaaatgcgt cttcaaggat tgaaaaaagc agaaaaagca gaaacgaatg
     3481 gtttggagat cctttatgat gtccaaaaat tgaaacaatt tactcaaaag ctaccttttg
     3541 aactgacaaa tgcacaaaaa cgtgtaacga atgaaatctg tcgggatctt cgcagtccgc
     3601 agcatatgca gcgacttttg cagggagatg taggtagcgg aaaaacagtg gttgccgcga
     3661 ttgcgttata cgctaccgtt acagcaggtt tccaaggtgc gttgatggtg cctacagaaa
     3721 tcttggcaca acaacatatg gaaagcttgc aacagctgtt tgatcccaat gaagtaacca
     3781 tcgcattgat tacggggtca acgaaagcaa aagaacgacg tgagctcctt gaaaaactag
     3841 agcaagggga aattaacgtc gtgatcggca cacatgcatt gatccaagat ggagtcgaat
     3901 ttcagaattt aggtctagtt attacggatg agcagcaccg ttttggcgtg aaccagcgga
     3961 aagtacttcg tgaaaaaggc tggcgaccag atgtcttatt tatgacagcg actccgattc
     4021 ctagaacatt agctattaca gcatatggtg agatggatgt atctatcatt gatgaacttc
     4081 cagctggacg tatcccgatc gaaacacgct gggtaagaac acctcaattg gacagtgtac
     4141 tggattggac gaaaaaagaa ctggcaagag gacatcaaat gtatgtcatc tgtccgctga
     4201 ttgaagaatc agaagcatta gatgtaaaaa atgcagtgga aatctatgaa aaacttcgtg
     4261 aactatttgc accaaaatat gaagttggct tgttacatgg taaaatgaag aatcaggaaa
     4321 aagaagtaat catggaagcc ttcaaagaca accagatgca gatattagtt tcgactactg
     4381 taattgaagt aggggtaaac gtgccgaacg cgaccgtcat gctgatcatg gatgcggatc
     4441 gatttggatt ggcacagctg caccaacttc gtggacgtgt tggacgcgga agtgatgctt
     4501 cctactgtat cttagttgct aatcctaaaa atgaactagg tgtggaacgg atgaaaatca
     4561 tgacagagac caccaatggt tttgtactaa gcgaaaaaga tctggaatta cgtggtcctg
     4621 gtgaagtatt tggcttccgt caatctggtc tgcctcaatt tgcgattgct gacttggtga
     4681 cggatggaaa tgttttggag gtagccagag aagaggctgc agccctttgg caaataaagg
     4741 attggcagat gcttcctgag tatcagcctt tagcgcaaag cctccaaata gaaaatcagg
     4801 ataatcgtta tttcgattag cagactgtgc tatactttat actgatgacg gaaaaaggag
     4861 tggaaacaat gaaaattgcc gtagatgcaa tgggcggaga tcacgcacct caagcaattg
     4921 tagaaggcgt ggctttagcg cagcaagatt ttccagaagt agaattctta ctttacggaa
     4981 aagaagcaga aatcaagaaa tacttaacaa atgaaaaaaa catcacgatt atccatacag
     5041 acgaaaaaat caatagtgat gatgagccag taaaagcaat tcgacgaaaa aagactgcat
     5101 caatggtcct tgctgcacaa gcagtgaaaa acggtgaagc agatgctgta ttttcagcag
     5161 ggaatacagg cgcgctacta gcagcagggt tgttcatcgt aggacgtatc aaaaatatcg
     5221 aacgccctgg cttgatgtct accttgccag tagtagggaa agaaggtgcc ggtttcgata
     5281 tgctggatct tggcgctaac gcagaaaaca aaccagaaca tttgttgcaa tacggtattt
     5341 taggttcttt ttatgcacgt aaagtccgag gtatcggacg tcctcgtgtc gcactcttaa
     5401 acaacggaac agaagcaaca aaaggtagtg aagtgacgaa aaaagcctat gaactccttc
     5461 agaatgaaac aagcttgaat tttattggaa acgtagaagc tcgtgagcta ctcaatggtg
     5521 tggctgatgt agtggtcaca gatggattta caggcaacgc tgttttgaaa tctattgaag
     5581 gaacagcaat gaatatgatg aatctattaa aatctgctat tctaaatgaa gggatcaaag
     5641 gaaaaatggg cgctatgctt ttaaaagacg gcttacgttc tttgaaagca gaaatggatt
     5701 attccaaaca tggcggtgcc gttttgtttg gcttgaaagc accagtcatt aaaactcatg
     5761 gagcaacagg ccccgatgct gttcgctata cgatccgcca aatccacaca atgcttgaga
     5821 cagatgtagt tggacaatta gtcgaacaat ttgaaaaaag cgaagaatag tatttggata
     5881 tctgaagcga tccgttccat tgaaaaaaag gtggacattc ctgaaaaatg ccagtaaaat
     5941 tagccatggt gtaggtaatt gacaagtgta tgattcttgg aggtgaaaaa gttgactcgc
     6001 gaagaagtat ttaacaaagt agcgaaaatt atttcaaatc attttgagat agataccgat
     6061 aaagtgacgg atgaattgaa catcaaagat gatttaaaag ctgattcaat tagtattatg
     6121 gaattcgttt tagagcttga agatgaattc ggtacggaaa tttcagatga agacgctgaa
     6181 caaattgaaa cagtcggtgg cgcagtagat tatatcagca gccatcttaa ataatttcaa
     6241 ataaaagcaa agaaggataa aaagctgcat gcagcttttt atccttcttt ttttaatctt
     6301 tttgacacgt aaaagtcatt tttctgataa ttctggcgat aaacagaaat tatgttgca
//
LOCUS       DANKAZ010000116         6303 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000116 DANKAZ010000000
VERSION     DANKAZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            334..714
                     /locus_tag="PWY67_002457"
     CDS             334..714
                     /locus_tag="PWY67_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL2058674.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="PWY67_002458"
     CDS             696..1610
                     /locus_tag="PWY67_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2058675.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="PWY67_002459"
     CDS             1855..2544
                     /locus_tag="PWY67_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058676.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="PWY67_002460"
     CDS             complement(2665..2751)
                     /locus_tag="PWY67_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2058677.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="PWY67_002461"
     CDS             3427..4377
                     /locus_tag="PWY67_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL2058678.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="PWY67_002462"
     CDS             4398..4931
                     /locus_tag="PWY67_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2058679.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="PWY67_002463"
     CDS             4947..5117
                     /locus_tag="PWY67_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058680.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="PWY67_002464"
     CDS             5567..6100
                     /locus_tag="PWY67_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058681.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2324 a         1102 c         1173 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtatacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DANKAZ010000117         6173 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000117 DANKAZ010000000
VERSION     DANKAZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            725..811
                     /locus_tag="PWY67_002465"
     CDS             725..811
                     /locus_tag="PWY67_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2058682.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="PWY67_002466"
     CDS             complement(932..1621)
                     /locus_tag="PWY67_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058683.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="PWY67_002467"
     CDS             complement(1628..1906)
                     /locus_tag="PWY67_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058684.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="PWY67_002468"
     CDS             complement(1866..2780)
                     /locus_tag="PWY67_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2058685.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="PWY67_002469"
     CDS             complement(2762..3142)
                     /locus_tag="PWY67_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL2058686.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="PWY67_002470"
     CDS             complement(3592..3879)
                     /locus_tag="PWY67_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2058687.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="PWY67_002471"
     CDS             complement(3899..4123)
                     /locus_tag="PWY67_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL2058688.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="PWY67_002472"
     CDS             complement(4578..4748)
                     /locus_tag="PWY67_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058689.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="PWY67_002473"
     CDS             complement(4763..5296)
                     /locus_tag="PWY67_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2058690.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="PWY67_002474"
     CDS             complement(5317..6159)
                     /locus_tag="PWY67_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2058691.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DANKAZ010000118         6133 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000118 DANKAZ010000000
VERSION     DANKAZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(207..1169)
                     /locus_tag="PWY67_002475"
     CDS             complement(207..1169)
                     /locus_tag="PWY67_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2058692.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="PWY67_002476"
     CDS             complement(1171..2610)
                     /locus_tag="PWY67_002476"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL2058693.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="PWY67_002477"
     CDS             2795..4741
                     /locus_tag="PWY67_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL2058694.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="PWY67_002478"
     CDS             5005..5877
                     /locus_tag="PWY67_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2058695.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1954 a         1056 c         1080 g         2043 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     6121 gttacactca aat
//
LOCUS       DANKAZ010000119         6033 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000119 DANKAZ010000000
VERSION     DANKAZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            12..530
                     /locus_tag="PWY67_002479"
     CDS             12..530
                     /locus_tag="PWY67_002479"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2058696.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            658..1341
                     /locus_tag="PWY67_002480"
     CDS             658..1341
                     /locus_tag="PWY67_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL2058697.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1650..1723
                     /locus_tag="PWY67_002481"
     tRNA            1650..1723
                     /locus_tag="PWY67_002481"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1684..1686,aa:Arg,seq:tct)
     gene            1882..2241
                     /locus_tag="PWY67_002482"
     CDS             1882..2241
                     /locus_tag="PWY67_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL2058698.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2364..3995
                     /locus_tag="PWY67_002483"
     CDS             2364..3995
                     /locus_tag="PWY67_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL2058699.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4057..4698)
                     /locus_tag="PWY67_002484"
     CDS             complement(4057..4698)
                     /locus_tag="PWY67_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2058700.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4884..4960
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4980..5288
                     /gene="rplU"
                     /locus_tag="PWY67_002485"
     CDS             4980..5288
                     /gene="rplU"
                     /locus_tag="PWY67_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL2058701.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5315..5659
                     /locus_tag="PWY67_002486"
     CDS             5315..5659
                     /locus_tag="PWY67_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL2058702.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5672..5965
                     /gene="rpmA"
                     /locus_tag="PWY67_002487"
     CDS             5672..5965
                     /gene="rpmA"
                     /locus_tag="PWY67_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL2058703.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT         1876 a         1014 c         1285 g         1858 t
ORIGIN      
        1 agttgaaaac gatacctgat tacaaaaaaa taacttttat aagttctata acgttgatta
       61 tatttttaat tcttattctt ctcgtcgtct tggttccccg aatttttggt ttgactcctt
      121 atattattga ggacaaagaa atgagtccat attatgcaca aggaaatctg atatatgtac
      181 gaccgaaaaa tacaaatgag attttagtcg gagatattat tacatattat gaaaattcag
      241 gaaaaaaaat ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa gatttttata
      301 caaaagctga tacaaagact tatattgagc ctggagtagt aaaaaagaga aatattatcg
      361 gtagtcccat ctttcagatt ccatatattg gactagtcct ttcaaaaacg gcatttgcat
      421 ggattcaggg gttattcttt acgattgctt tttgcctaac aggaatcaca gcatggaata
      481 cctttgtaga gttgaaaaag aagcggagac gaacacagaa atttttgtaa gaatgttaaa
      541 aacataaatg tattccagag atccaacgac tcttctgaac atgaaacacg agaaaacact
      601 catgcagctt gtagatttag tcagttgttc gtataatgtg tgaagaggag gagtagtatg
      661 cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac tattggattt tatgcgtacc
      721 cagcaaaaaa aattaccggg tgagctaggc aaactggaag aagaagctca tgtagcagaa
      781 gtaccagtta ttccccatga aacagttgta tttttgaaat ttttacttgg acagcttcag
      841 cctgaacgaa tcttggagat cggagcagct atcggttttt catcaagtgt catggccacg
      901 acaatcggag aaaacgctca tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa
      961 gctaaaaaaa actatgaacg tctaggactg acagataaag tgactttgct tgaaggtcaa
     1021 gcagcagata tcttgccgga gttgtctggt ccgtatgact ttatttttat ggatagtgca
     1081 aaatcaaaat atattgaatt tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt
     1141 ttgatggtag atgacatctt ccaaggagga acgatcttgc tcccagatga agagattccc
     1201 cgtggcaaac gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat
     1261 cccgatttga catcgacaat cttgccatta ggtgatggtg tgatcttaat gacaaaggaa
     1321 tcagaaacaa tcgagttgtg atacatgatt atggcagcct gtagataatc gattgaatcg
     1381 agggctcaat tcgaacttga tgtaaaagtt tctatatttg taagtcaatt aaaggataca
     1441 ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt caattgataa
     1501 ccttagaaca gctgaaggag aatgtctcgt tttaaaagat tgatttagta gttgggccaa
     1561 aataattcat tatttgctaa ttaaataact atttcgtatt gacagaagca atttaattat
     1621 ctataattat tgatgttccg ctgattcttg tcatagtagc tcagctggat agagcatccg
     1681 ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa accgttagaa
     1741 actgaggttt ctaacggttt ttctttgttt tttgtgccaa tattgtttgt tttcaaaggc
     1801 aaataaaata aaaaaacatc cttatcgagt acaattataa ggagcaaaaa aataaaactc
     1861 atcagaaaag aggaagaaga tatgacgata ctatctagaa ttttagtgac tctagttgca
     1921 attgaatttt tttatatcat gtatatcgaa acggtgagaa ctgattctga cacgacaagt
     1981 agggttttta aaatgagtaa agaagagtta agcagaaaat ctgttcaaac attgtttaag
     2041 aaccaaggag tgtacaatgg actattagga gtgggactac tttatggagc ttatctttct
     2101 tccgcatcta aagaaatcac aagtatgcta ttaatatcta ttttttttgt tgcattatat
     2161 ggcagtttga ctagcgatcg ttggatttta ctcaaacaag gtgggctagc tctgttggca
     2221 ttgctttcta ttttcttata aatatagaaa aaataagaat ttatctaaca ggaaactttg
     2281 tgaaaaatat tactttatct catgaaatat gatattctaa tgaatggtat tttagaaaat
     2341 ttcatgaaaa aggagttaga taggtgttca aaaattatgt ttcctcacta aaaaaagaat
     2401 tttcaggtta tgatctttca agatttcaga aagatctttt ggctgggatc acagtagctg
     2461 ctgtagcatt gcctttagcc ttggcttttg gggtatcaag cggagctgat gcggccgcag
     2521 gtttgattac ggctatcatt gccggtatcg ttattggcgg tttatcaggc ggtttttatc
     2581 aaatatcagg tccgacaggt gcgatggcag ctatcttgat gtctattgcg gcaacaaagg
     2641 gaatgcaagg tgtattgctt gctacttttt tagcgggtct atttttactt gcagcaggac
     2701 ttttccgatt aggaacactg acttctttta tcccagcacc cgtaattacg gggtttactt
     2761 caggtattgc tatcattatt gcattaggtc agatcgataa tttatttggt gttcactcgg
     2821 aaggtacaaa cgttattgaa aaactaagta gttatcaaac gataggattt ccgataaaca
     2881 cagcctcttt gatcatggga gggctagtca ttttagggat gtttatctat ccaaaaaaat
     2941 gggcgaaacg aatgcctagt tcgctgctgg ctatcatttt ggcaacagca gttatgctat
     3001 tgttccattt accagtagca actgtgggta agattccaca aacattgatc agtagtaatc
     3061 gtttgaatat gggagatttt agcttttctg ccttgcaaca agtggcagtt ccagctatta
     3121 gcattgcttt attaggaatg attgaaagct tgctatgtgg tgcttcggct ggccgaatgg
     3181 caaataagcc cttagatagc aatcaagaat taatggcgca aggaattgga aatctgttgc
     3241 ttcctttctt tggcgggatt cctgctactg cggccatcgc ccgtacaagc gtggccatca
     3301 aatctggtgc acaaactaga ttagctggta tgatacatgc agttgtctta ctgctctcca
     3361 tgcttgtttt tgcgccagtg atgtcacaga ttccaatgcc agcacttgca ggtgttttga
     3421 ttgtgacggc ttggcgtatg aatgaatggg aaacgatcaa ggagctattt actaataaat
     3481 attggtcagc agttctattg tttatcctta cgatgatttg tacagttatc tttgatttaa
     3541 gtattgctat cgtgatcggg gtgatcggcg gctgtgtatt ttttgttgtc aaaagcgctg
     3601 ctatcacgat ttcattagaa acgatcgatt ggcaaagaat ggggctgcca gaaacggaaa
     3661 agctgaataa ctggacagta gtgtatatta gtggtccact gttttttatg tcttcagaaa
     3721 aactcaaaaa taaattggaa gaactaaatc atcacgatgg tgtcattttc tccatgcgag
     3781 gcgtaccaag tattgatttg acggcacgtg cagtttttga tgaatttcaa gaaaaagcag
     3841 agcaaagagg acaaacgatt atctatactt ccttgcagcc agaagtagaa aaacaacttt
     3901 ctcacttgtg ggaacataca ttcacgcatc aacatcaaac agttgctcat gcgttgacag
     3961 ctttacatgc gaaatatact gcaattagcc attaaaacaa tgaaaaagaa cagacaagac
     4021 tgtaaactga agattttatg gtcttgtctg ttctttttaa tattgctgta aaagtgaagc
     4081 tagtacttgc cagtcttgat ggataacagg tacaagggag cgattgtaga aaatagctgc
     4141 tggatgaaaa agtggaagaa ttcgatagtt tttttctgac cattcgtagg catccttttg
     4201 ttctgttaat tgtaagacag gccctgtata gagttgacca tgatttttgg taaccgtata
     4261 ttctttgcca agtaaacgtt gaagaccaat gtttccaagt gtcgcaatta attttggctg
     4321 gaccttcatc agttcgtaat ccaatattgg tgcatgcgca aatacttcgg aacgagtagg
     4381 tgtccgattt ggataaacag tttctgactg ttgtgtccgt ttattgatcc gatgagtcac
     4441 gcgatacggg cggctcctta cagcactggt gatatacacc tcttctcgtg ttaagccagt
     4501 tgaagaaagt gcctgcatca attcttttcc agcttgaccg ctaaaaggaa catgcgtatc
     4561 aatctctgtt ttccctgggg cttctcccac gagcatcagt ttaggatgaa gaggtccttg
     4621 cccagcgata aatccttcta caggacggcc gttcattctt aaagaagctt cttgtatcag
     4681 ttcttttggg tattccattt atgctcctcc tttttctttt attataagaa taaaagaaac
     4741 acaatgcgac tattttattt ggtagaactg tgaggataga aaagcagaag tattgcttga
     4801 atttttgttt tactttttga ttttaaattt agttgactaa cggttgctgt tctggtaagc
     4861 tagtactgtt gtaattgtgg actccacagc tacaaccgct cagaacaagt aataagcaca
     4921 gcatatgctg tcacttcgga tggcgagtct aagtatatca gaaggaggtg ctttatagca
     4981 tgtacgcaat tattaaaact ggtggtaaac aagtaaaagt tgaagtggga caagcaatct
     5041 atgttgaaaa acttgacgtt gaagcaggag aaaaagttgt ttttgacgaa gttatcttag
     5101 taggcggaga atctacaaaa gtaggcgctc caactgttgc aggtgcaact gttgaaggta
     5161 ctgttgaaaa acacggcaaa caaaagaaag tcgttacttt caaatacaaa cctaaaaaac
     5221 attcacaccg taaacaaggt catcgtcaac catatacaaa agtggttatc gacgcgatca
     5281 acgcttaata gcgtttgaga aagaaggcct atatatgatc aaaggaacat ttaagcgaaa
     5341 tgacgcaggt cagatcgttt cattcacttt gaccgggcat gctgatgctg gtccttatgg
     5401 aagtgatatc gtttgtgcgg gtgtttctgc gttagcgatc agcactgtca acgggattgc
     5461 ctcattagcc ggttttgagc cgattgttga aatgaatgaa gaagaaggcg gttatcttta
     5521 taccgaagtc acttcaggaa tgacacaaga acaaaacaat attgctgaga ttctattaga
     5581 aaatctttta ttaggattac aatcgataga agctgaaaac tcagaatata tccaaattaa
     5641 aacaattaat gataaatagg aggtgcagac catgttgtta tctatgaatc tacaattatt
     5701 tgcccacaaa aaaggtggcg gttctacatc taacggccgt gactcagaat caaaacgtct
     5761 aggtgctaaa cgtgctgatg gacaaacagt tacaggtgga tcaattcttt accgtcaacg
     5821 cggtacaaaa atctatccag gtgtaaacgt gggtatcggt ggagacgaca ctttatttgc
     5881 aaaagttgat ggcgtagtac gtttcgaacg taaaggccgt aacaaaaaac aagtgtctgt
     5941 ttatccagta gctcaagaag cttaattgaa tcttatgatt aaagaggttg tgacaagact
     6001 tttgtcacag ccttttttgt ttataaaaat aaa
//
LOCUS       DANKAZ010000120         5892 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000120 DANKAZ010000000
VERSION     DANKAZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(103..588)
                     /locus_tag="PWY67_002488"
     CDS             complement(103..588)
                     /locus_tag="PWY67_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058704.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(727..942)
                     /locus_tag="PWY67_002489"
     CDS             complement(727..942)
                     /locus_tag="PWY67_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL2058705.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1166..1966
                     /locus_tag="PWY67_002490"
     CDS             1166..1966
                     /locus_tag="PWY67_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2058706.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1985..3853
                     /locus_tag="PWY67_002491"
     CDS             1985..3853
                     /locus_tag="PWY67_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2058707.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3846..4337
                     /locus_tag="PWY67_002492"
     CDS             3846..4337
                     /locus_tag="PWY67_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL2058708.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4325..4879
                     /gene="srlA"
                     /locus_tag="PWY67_002493"
     CDS             4325..4879
                     /gene="srlA"
                     /locus_tag="PWY67_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL2058709.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4897..5892
                     /locus_tag="PWY67_002494"
     CDS             4897..5892
                     /locus_tag="PWY67_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL2058710.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2058 a          943 c         1188 g         1703 t
ORIGIN      
        1 taggttaatg caaatgctta tttatactcc gtataacttt ctcagaaaaa atgataccaa
       61 taatgacttg ctagtactgt aaatccgtat aatatcatct aatcaaaatg gtgttccatt
      121 tgcatctgtc atcgtaatcg atccttcggg tgacactcga taaaaaccaa cgcttccaga
      181 tcctccttgg gaacggattt gtttgctgga agctgtgaac aaataatctc gatcaatttt
      241 ttgataaaag ctaataacaa tatcgtcact ttgtaattcg gaacggtggg caataagaaa
      301 tagaagtgct ttgtcagagt tttctaatgg aagagaaggt acagtgcttt cttgcatttc
      361 agatgttgaa gaaatgctaa acgactgaat acttgattgg ctatgttgcg aatctacatt
      421 accagtagaa gtattagtaa tggaagtagt agaagtactt ttagttgaac caacctcagt
      481 tgttgaaatg gctgctgaag attctttaac agaattcttt gttttatatc cacaggcaga
      541 aaaaagtatt acagttccca acaagcctgc gtaaatccct ttatgcatga tagctcctcc
      601 taaaatagat tatctttatt cagtatatca cttaaacaaa gaaaagcaga tggtttaaaa
      661 tgaaaaaacc ttcccaataa agggaaggtt catgaagata ataacttttt taataattat
      721 agctttctaa tatctttcat caacttttct agtaatcatt gggaaaaatt tcaagcagta
      781 gatcgcacaa atacctacga cgatataaat gatcttcgca aaaatagttg ttgaaccacc
      841 agcaatcata gctactaaat caagttcgaa caagcctacc aacagccagt tcaaccctcc
      901 cacaatcaat aaagttaatg caatagcatc taaagttttc attagaattc tcctttctta
      961 aatgataaaa caattatata tcaaaaagag aaacattgct tatataacgc tcaggaattt
     1021 tagcaatcta tgcaaaaaga tgaaaatttt ttttgctctt ccattagtac acatttttta
     1081 gctgaagctg taaatttcat gtcttaattt tttttagtga gcttttatac tggatttgta
     1141 agaaaaaaga aggagaggat cacatatgtc ggattggttg gatattgcag gaaaagtagt
     1201 aattgtaact ggaggttcct caggaattgg aagaagtatc gtcgaaaatt tgttgaagca
     1261 gaatgcacaa gtagcgaatt ttgatgtaac tgaatgccgt atacagcatg aaaacctact
     1321 atctttaaaa gtcgatgtgt cttctaaaac ggacataaaa gaagggatat ataaagtaat
     1381 gaaacatttt aagacgatcg atggtctagt caataatgca ggaataaata ttccttctct
     1441 tctaatagat agaaatcatc caaaaagcaa gtatgaatta tcagaacaag tttttgataa
     1501 gatgatcgct gtcaatcaaa aaagtgtata tttgatgagc caagcagttg gaagaatctt
     1561 ggtgcaaaag ggatcaggag tgatcgtgaa tctttcttct gaatcaggat tggaaggatc
     1621 agaaggacag agctgttatg ctgctacaaa agcagcgatg aacagtttta ctcgttcttg
     1681 ggcaaaagag ttaggaaaga ggaatgttcg tgtggtaggt gttgcaccag ggatactgga
     1741 agaaaccggt ttgagaacac aagaatatga agaagctctt tcttatacga gaggtataag
     1801 cgtcgaacag ctgagaaacg gttacagtcg aacaagtacg atccctctag gaaggagtgg
     1861 taagctgcaa gaagtggcgg atctagtctg ctattatctt tcagaaagat caagttatat
     1921 cactggagtc acgactaata tttcaggagg taaaacaaga ggataaagga taggggggac
     1981 gcacgtgacg ctagtaaaca gatggtacca attattgaaa ttactagtca ctcacaagaa
     2041 agtttcaatc caggaagtgc aaaaagaaat caaaaccagt aatcaaacca taaaaaaaga
     2101 tattggggag ttgaatgaag aaattgccgg tattgcaaaa atcatccaaa aaaacaaaca
     2161 ttttgaactt gaaatttatg attttgattt ttttgatgag atcatgcaag gaaaattgaa
     2221 aacagccagt gatttcaatt cggcatccaa aagaatcgct tatttactaa ataggctgat
     2281 cgaaacagag gatttcttac gtatggatga tttgtctgaa gaagtcggtg tcagcagagg
     2341 aacagtggtc aaggatctaa agacattaaa agagatgatc aatgattttg atgtaaagat
     2401 tacaggcaca ccaaataaag gactaagaat aataggagat gagctagaat tacgactact
     2461 tttattttat tttgtttctc cctacttttc agagacgaaa acagaacagt ttctagaaga
     2521 aaaacaagca atcaaacatt ttcagaagaa gacgaatgct agtaaacaag aaatcaccct
     2581 tttaacgaaa gttatcggta tttctttgaa tagaatctct agtaactatc tgttgaagaa
     2641 gcccatcaat cgttacagtg ggtgttttga atatatccaa gaattcaatg aactgatcaa
     2701 tcagttggaa gaaatctatg aactaacttt gagccaatat gaaaaagatt ttatcagttt
     2761 tcctttgaac atcatcaaca caagtataac gatgataagg aaagctaact ctgatttgcg
     2821 cttatatttc gaccccatga tgatgcgtat tcgacgatta gatctcattg atctggatga
     2881 gtccttctta tatcaagaga tgaaacagca cctctcttta ttgataaatc gggtaatttt
     2941 tcgttatcga ttgactgatt tattttatgg tgagatagaa aaaaagtatc ctttttctta
     3001 ccaaatcgct agcgaatgca tacaagcatt agaagctatc ttgcaacata agatctctaa
     3061 agtagagatc agctacctaa cgttgtattt ggagttagca tttcgatatc aaaaagagca
     3121 tttttcctct aaaaaagtag ccattgttca tacgacagga aaaggaacag cattaatgat
     3181 ccaaagacaa atcaaaagga tattggggag ggatatacaa ataacacatg tttcagaaga
     3241 acaatatgaa aaagtagatc taaacaaata tttcgctgtt tttacaacga ttccgttaaa
     3301 aaatgtaaat gaagagacac cggtgatcca tttgactaat ctttttgatg actcttggtt
     3361 aagggcacaa tggcaaaaag caaaaaaaaa gcgattagcc gggtttcatt ctactgtttt
     3421 tttctatcaa atatggaaag aaaaaaagac gtatcaagaa tatttaaaca aattagtcgc
     3481 tcctttgatc agaggctatt atgtggatga agaatttgaa aaaaggatct tgaaaagaga
     3541 atatcagcaa agcacatttg ttaacaaaga atttgctttt cctcacacaa ccaaccagct
     3601 atctcaacac attgttttat cagtagggct aataactgat caaggtttag cagaaacagg
     3661 aggaaatgga gttatttttt tatttgccat acctgaagaa atgacggctg aagttgagaa
     3721 tgaattattt gagctatacg atatcctatt tgaactcata aaaatgggaa tagatgagtt
     3781 tattgaatta gcaccgacac aagaatcatt tttaaaacta atcaaaagta aaggagagag
     3841 acttcatgaa tgatatgtat ctattcatag ttttcgcagc ttcagcttat gtgctgcaag
     3901 cagttatcag ttggaagcaa ctaaagcatt tcaatcaaac atatagcgaa ctgcgaagaa
     3961 aaggaaaagt agcgattggt aaacgacctg ggaaaatccg ttcaggcacg attgttatgc
     4021 tagctataga cgatcatgat caagttttag aagtcagaaa aatgcaaggg gttacatctt
     4081 tggcaaaatt tcattcatta cctgtctatg cgggtgaaga tatccattac atcgatcgtt
     4141 accatccctc agttcgagcg gaagatcgtt tgacacaagc agctttagaa aatgccagag
     4201 aagtatactt acgaatagag gcaggaaatg aaaaaagtga acaaccttca ttgcctttta
     4261 ttgctatgct caataatttt cggatatgga aaagtacgat caaagaaaaa aggagtgaca
     4321 aacgatggat atcataacaa aatttgctga gggatttatg aggctgttcc aaacaggtgc
     4381 agaaacattc ataagctgga tgacaggaat cgtaccagtt gtcttgatgc tgatggttgc
     4441 aatgaatacg ctgattgctt ttttaggtga agaccgagta acgaaagtag ccaaattatc
     4501 agcgaaaaat ccgcttactc gttaccttgt gttaccgttc ttatctgctt ttatgttggg
     4561 aaatcctatg tcatttacaa tggctcgttt ccttccagaa tactacaaac caagctatta
     4621 tgctgctcaa gctcaatttt gtcatacgag caatggtgta ttcccccata ttaatcctgg
     4681 agaattgttt gtatggatgg ggattgctca agggatcgaa accttagggc taaattcgat
     4741 ggagctggct atccgatact tgcttgtggg actgctgatg aactttattg gtggttggat
     4801 aactgatttt actactggat ttgtatgcag acagcaagga atcgttctta gcaaaaaagt
     4861 agagctttca gttgattaga gaaaggagag ttttaaatgt atggtgatat taagatcgta
     4921 agaggaaacg gcgggtatgg aggtcctttg attattaggc cgacaaaaga taaacacaag
     4981 ttcatctatg tcacaggtgg aggtgagagg ccaatgatcg tagataaaat agaagaactg
     5041 acaggtatgg agtctatcaa tgggttcaaa acatcaatac ctgatagtga aatcgcgctt
     5101 gcaatcgttg actgtggagg gactcttaga tgtggaattt atcctaaaaa agggatacca
     5161 accatcaata tcgttcccac aggaaaaagt ggcccattag cacaatatat cactgaagat
     5221 atttatgttt cagcagtagg gttagaacaa atacagccac taatggaaac tgatgataac
     5281 aaagaaacag taaatactgc atctccagat aaaaagtaca aatatgatac ttctaaaaag
     5341 ataaccgagc aaaaagcaga gcagcaaaac tggatagcaa aaattggaat gggcgcggga
     5401 aaagtagttg ctacattcaa tcaatcagca agagaagcag tagatacgat gatccggaca
     5461 gtgatccctt ttatggcttt cgtttccctt ttgattggtg tgatacaagg ttctgggatc
     5521 ggtaatgtat ttgctaacat tatggcacct ttagcaggaa atgcagttgg attaacaatc
     5581 attggcttta tttgttcact gccattttta tctcctttgc ttggtccagg ggcagttatt
     5641 agccaagtga ttggaacatt gatcggtgta gagattggga aaggaaatat tcctccccaa
     5701 ttagcattac cggcactatt tgctatcaat actcaaaatg catgtgattt tattccagtc
     5761 ggtctaggtt tggaagaagc aaaagctgag acagtagaag taggtgtacc atcagttctt
     5821 tattcaagat ttcttaacgg catcccacga gttatcattg cttgggtagc aagttttgga
     5881 ttatatagct ga
//
LOCUS       DANKAZ010000121         5809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000121 DANKAZ010000000
VERSION     DANKAZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            450..1604
                     /locus_tag="PWY67_002495"
     CDS             450..1604
                     /locus_tag="PWY67_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL2058711.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="PWY67_002496"
     CDS             1598..2020
                     /locus_tag="PWY67_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058712.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="PWY67_002497"
     CDS             3143..4075
                     /locus_tag="PWY67_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2058713.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="PWY67_002498"
     CDS             4092..4607
                     /locus_tag="PWY67_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2058714.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="PWY67_002499"
     CDS             4903..5184
                     /locus_tag="PWY67_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058715.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="PWY67_002500"
     CDS             5186..5602
                     /locus_tag="PWY67_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058716.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DANKAZ010000122         5809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000122 DANKAZ010000000
VERSION     DANKAZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..1063)
                     /locus_tag="PWY67_002501"
     CDS             complement(<1..1063)
                     /locus_tag="PWY67_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2058717.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEK"
     gene            complement(1074..1742)
                     /locus_tag="PWY67_002502"
     CDS             complement(1074..1742)
                     /locus_tag="PWY67_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2058718.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1872..2456)
                     /locus_tag="PWY67_002503"
     CDS             complement(1872..2456)
                     /locus_tag="PWY67_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2058719.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2787..3020)
                     /locus_tag="PWY67_002504"
     CDS             complement(2787..3020)
                     /locus_tag="PWY67_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDL2058720.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3035..3985)
                     /locus_tag="PWY67_002505"
     CDS             complement(3035..3985)
                     /locus_tag="PWY67_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2058721.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3982..4824)
                     /locus_tag="PWY67_002506"
     CDS             complement(3982..4824)
                     /locus_tag="PWY67_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2058722.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4826..5545)
                     /locus_tag="PWY67_002507"
     CDS             complement(4826..5545)
                     /locus_tag="PWY67_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2058723.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1962 a         1040 c          727 g         2080 t
ORIGIN      
        1 ttttttcttg ctgatcttta ctaactattt cattccccag ggctaactcg ctcctcactg
       61 attctgtgaa aaattggtaa tccatttctt gcattactaa aaacggcgat gacaaatttt
      121 ttttattttc gattatcttt cctttggtag ggcgaataat cttcatgagt attcttagta
      181 aggttgtttt ccccacaccg tttttcccta ccaaaccaat aacatctcca actttgacat
      241 caaaatttat gtcttttaga atattttcat aagtaatttc ttcaactttt aatacatctg
      301 cacttacctt ttcgatatag ggaacttcaa tcgaactatt aaaaatgtca aatttcctta
      361 atgcgttatt gttttcagtt tttaacaact gaatagttgt agtttttaca gttctatttt
      421 taacaaatag aacttgatca attaattctt tcaaatagta aaaacgatgt tcagctataa
      481 caatagtaaa tcctttattt tttaaatcac aaagtaagtc agcaagtttc ttagtcgctt
      541 tgaagtccaa atttgaagac ggttcatcca atataattaa tttagagttt agcgataaac
      601 tacatccaat agaaattttc tgccgttcac cacttgatag cgaaaaaatg tttttatcta
      661 atatatttga aagatctaat aattcagcta attcattgat tcttttatcc atatgctctt
      721 ttgtaaatcc aaagttttcc atcgaaaata ctaactcact cgttgtattt gttgtaaaga
      781 attgtcctct aggatcttga cttacgtagc caatattttt tgatatactt tcttgtgtta
      841 aggattgtag cgagttattt tcaaataaaa tttccccaac caattttccg tcataaagtc
      901 ttggaataag tccattcatt aactttaata gtgtggattt cccactccca gaagaaccag
      961 taatcaatgt aagactgcct ttttttattg aaaaattaag gtcaacaagc gcttgttcct
     1021 taacatcctt atatgaaaaa gaaacattgt taaattcaaa caattctttc acctcaaatc
     1081 aaaatactta agaaaaaaat agtaacggaa aacaggatga tatagtctaa taagctcatg
     1141 tttgttttat agtaagaact tcttttcaca ggagattcta tcccctttgt tattgcatta
     1201 gcggtcattt cttcagcaat catagttgca cgtataagaa cgggaactaa cagaaattcc
     1261 aaatattgca gaggctgaaa aaaagacaag cgaatgtttc tcatttttaa agattcttta
     1321 atcattctta tttcagaacg ataagtcgga aagaatctga tcactactgt aatacttaac
     1381 attatttttt tcggtaaatg tattttttct aaagaggata ataattcatt gctagtatag
     1441 acactaacca acaaccatga gatcattacg accggcaaca ttcgaaatac aatatataag
     1501 gatgtcgcaa aaaatatagt aaaaaactct atcttgatag catcaataat tttcattact
     1561 aaaaacagaa ctgaataaat caagatgaaa taaatacatg tctttatttt acgaaacatc
     1621 aatactaaaa ctgtagcaat aatgaaacac cccacttgaa atatatttcc ctttcctaaa
     1681 aaaataatga ccggaaaaaa aatatttaaa gtaatcaagg ttaacggatt gatttttgac
     1741 actctttcac ctccaaaaaa taagatcgca ctgttatctt aagctgataa aaatgcaccg
     1801 ttattcagat aacagtgcga tcttatcaaa aagcataact gagaacaatt ctcaatgcaa
     1861 gcttttttta attaactcag taattcaatc cacccaggaa cagaaaattg atacaatctt
     1921 ctggttaata gcatagtctt ttcttttgaa tagctatgct tcaatatatc atagaatgta
     1981 gcgtagaatg agtaaatgta gaagtggatt tcttcttcat taaagactaa attttgattg
     2041 atcattccga tagtctttaa cttatctaga caacagactg tctttttcgt cacaaatgat
     2101 gtaatttttt caacaatatc ttgatatttg gttccagctg attggaataa aatcaaatcg
     2161 aatatacgtt tattctcaaa aatataatta ataaatggat ctaattcatt gaagttctct
     2221 ctccaacttt tctctatatc tatattttct aagtttaaga ggtatttggt aaactcttca
     2281 ttatacatat taataagcgc attaatatgc ggctgaataa tttcttcaaa taacgcttcc
     2341 ttagaagtaa aatgtttata aaaagcaccc gtagttacgc ctgcggtcag gcacagttgc
     2401 cttatcttag tttgctgaaa tccttctttt tcaaaatggc ttagtccact ttctattaac
     2461 ttttggtgag ttataatata atctctggtt tctatgggta cttcctccta ataagatgaa
     2521 ttaactttat tatcttacac aggacttgaa gtttcaatcg ttaacgttta ttactgaact
     2581 cattccttag agctaaacta atgagaaaga gcagaataac tgatacaaaa agcaatccta
     2641 caagaattaa tagactcatc taaatcattc accttttcta aaataatccg agaaagattt
     2701 aaaaaaatta tatccagcta ttaggataag tcctatcata atcactccaa tagccatttt
     2761 cttatcctcc ctaaaaataa agtcttttac ttaaaacctt ttttgtaatc gtcaataaat
     2821 tttttccctt tatcagttaa ttgaaatcct atatctaaat agtcctcata tctaagttta
     2881 gccagcatct cacttacaga tgctggacta acacttagaa cttttgctaa atcattattt
     2941 tttaatttct tttttctttt tgataattcc tgaattgcta gtaaataaat tagtatagta
     3001 gtcgttttaa tttgtaccat acctaccctc caaattattt cgaaaggcct tcagcaattt
     3061 tatccaggtt ccatttcatc atcgcatagt aactatcacc atcttgtcct ttttctgcaa
     3121 ttgaatcagt aaaaatcgtt gaatagatag gaatattggt atcttttgat actgttttca
     3181 ttggtctatc atccacactg ctttctacga ataaggaggg aacttttgtt gtgcgtaatt
     3241 tttcaactaa gtgttttatt tgatctggtg ttccttcttc ttcagtgttg atttcccaaa
     3301 tgtaagcaga aggcacatta tacgctttcg agaaatattt aaagcatcct tcacttgtaa
     3361 caatcatttt tttatcattc ggaattgaag caaatttaga tttagcttct ttgtctagag
     3421 aatccaattt ttcaatatac ttatctagat tttctttata gaatttttta ttatcaggat
     3481 ctttttccgc taactgtttt tcaatatttt tagcgtaaat aataccgttt tctaaattta
     3541 accaagcatg aggatcttcc ttccctttct cactctgacc ctctaagtaa ataacatcta
     3601 tgccatcact tgctgcaaaa tagtctttgt tttcctcttt gttcgcattt ttaactaatt
     3661 ttgtaaacca agcatttcct ccagtttcca agttaacacc gttataaaaa atcaaatctg
     3721 cttttgaagt tttttgaaca tcttcaggca aaggttcata ttcgtgggga tctttcccaa
     3781 taggtacgat actgtgtaaa tcaattttat cttttgctat attttcggta atatctgcta
     3841 aaatcgaatt agtaactact acttttaatt tttcgttcga agccacttta tctttgtctt
     3901 tactatttat tgaacatgcc ccgaatataa aaagagcaat caaaatcccc agacttccca
     3961 gtactgcttt ccattttctc atcataacga tctctccttc tttcttaaaa atttttgttt
     4021 tggtgagatt agaaaactaa ttacaaataa gaatgccgct gttaaaacaa tacttgatcc
     4081 tgctgctatg ttaaaactat aaccaataaa caatccaagc aatgatgaac ttgctccgac
     4141 tgttgcagaa ataaatatca tagttttcaa gctatttgca tataaatatg ctgtagctgc
     4201 tggtgtaatt aacattgcaa caattaatat agttcccaca ctttgcatag ctgttactgc
     4261 tactaaagtt aatagaacca tcaatagata atgataaaaa ctgacattca tccctattgc
     4321 ttttgccatt aatggatcaa aagaagtgat taataattct ttaaaaaata gaactactat
     4381 aaccaacact aaaaccgaaa cgccaattgt aatccactta tcaatatctt gtacagctaa
     4441 aatatttcca aataaaatat gaaataaatc cgttgagcta tttgcaactc ctataaggat
     4501 aactcctagt gctaaaaaag aactgaaagt aataccaatt gctgtatctc cttttattac
     4561 actattgctt tttataaaag taataatgat agaagctaat agaccaaata caatcgcacc
     4621 aataaaaaaa ttaataccta aaatataaga aattgctact cctggcaaaa cagcatgaga
     4681 aatagcatca cccataagag acattcctct taaaataata aaacaaccta ctgttccaga
     4741 aacaatccct ataactacag atgtaattag agcattctgt aaaaagtgaa agtcatacag
     4801 accattaata aaattttgaa gcatattatt ccccctctcc aataaatata gtatctccat
     4861 aggctttttt taaattttct tcattaaata cagattctac acttccgtga gcaattagat
     4921 ttcgattcaa aagaataata ttatcaaaat acttctttac cttgcttaaa tcatgatgca
     4981 caattagcac tgtttttccc tgatgtttca atttttttaa cgtatccata attattcgtt
     5041 cactaactaa gtctatccct acaaatggtt catctaaaaa tatgaaatcc gcattttgag
     5101 caagacatct tgctaaaagt acacgttgaa attgtcctcc agataattct cctatttgat
     5161 gattagaata ttttcccata tcgacctttt ccaaagcctt tgaaactctt tgccattctg
     5221 catttttaat tctttgaaaa actttcattc cggcatatgt tcccatagat acacattctt
     5281 ttacttttat tggaaaagta aaatcaatat cagctttttg ctctacatat gcaatcttca
     5341 ccagtttttt attgatatct tcattattaa tggagacctc tccctgatga gggataatat
     5401 ttaacattgc tttaagcaat gtggattttc cagcaccatt tggaccaata atacctgtta
     5461 aagcaccttc ttgtattttt aaagaaatct cgttcaatgc taaaaaatcg ttataacaaa
     5521 cattaagctt tttcacttct aacataactt tctccttcta aatgataatg attatcacga
     5581 tcatatggta tacaaaaatt atattttagt caaccctaaa ttttatagtt tagatttttt
     5641 tctgttgctg aagattagaa tgacaaaact atttgaaact acgcgtatag taagtagctt
     5701 tcaaaatata taaaaaagga gcgttctcaa atgagaacat gctcctaaaa ctactttatt
     5761 ggcgtatgga aaaattatac ttgaagtgga tagcgggttg aataagttg
//
LOCUS       DANKAZ010000123         5780 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000123 DANKAZ010000000
VERSION     DANKAZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(525..1028)
                     /locus_tag="PWY67_002508"
     CDS             complement(525..1028)
                     /locus_tag="PWY67_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2058724.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1387..2412
                     /locus_tag="PWY67_002509"
     CDS             1387..2412
                     /locus_tag="PWY67_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2058725.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2735..3613
                     /locus_tag="PWY67_002510"
     CDS             2735..3613
                     /locus_tag="PWY67_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDL2058726.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4089..4205
                     /locus_tag="PWY67_002511"
     CDS             4089..4205
                     /locus_tag="PWY67_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2058727.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5133..5471
                     /locus_tag="PWY67_002512"
     CDS             5133..5471
                     /locus_tag="PWY67_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058728.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5565..>5780
                     /locus_tag="PWY67_002513"
     CDS             5565..>5780
                     /locus_tag="PWY67_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058729.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYV"
BASE COUNT         1966 a          875 c         1062 g         1877 t
ORIGIN      
        1 aataacatat ctcattttac gcttttgtta ttttatactt aaaaaataac gacacagaaa
       61 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      121 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      181 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      241 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      301 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      361 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      421 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      481 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      541 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      601 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      661 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      721 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      781 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      841 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
      901 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
      961 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1021 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1081 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1141 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1201 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1261 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1321 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1381 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1441 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1501 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1561 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1621 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1681 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1741 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1801 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1861 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     1921 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     1981 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2041 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2101 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2161 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2221 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2281 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2341 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2401 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2461 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
     2521 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
     2581 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
     2641 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
     2701 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
     2761 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
     2821 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
     2881 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
     2941 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
     3001 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
     3061 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
     3121 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
     3181 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
     3241 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
     3301 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
     3361 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
     3421 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
     3481 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
     3541 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
     3601 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
     3661 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3721 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     3781 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
     3841 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
     3901 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
     3961 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
     4021 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
     4081 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
     4141 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
     4201 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
     4261 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
     4321 atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat
     4381 agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt
     4441 caattccctt tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc
     4501 tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt
     4561 tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc
     4621 attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca
     4681 tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac
     4741 tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga
     4801 ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg
     4861 aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag
     4921 tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg
     4981 tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat
     5041 tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta
     5101 ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac
     5161 atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg
     5221 agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata
     5281 tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag
     5341 agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg
     5401 acgagactga gatcatttta acggattact caaaaattgc tactgataaa aagtatatat
     5461 tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga
     5521 tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg
     5581 gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga
     5641 aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag
     5701 gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag
     5761 aagagttttt ggattatgtt
//
LOCUS       DANKAZ010000124         5699 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000124 DANKAZ010000000
VERSION     DANKAZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            178..1128
                     /locus_tag="PWY67_002514"
     CDS             178..1128
                     /locus_tag="PWY67_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2058730.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            1646..2101
                     /locus_tag="PWY67_002515"
     CDS             1646..2101
                     /locus_tag="PWY67_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2058731.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2442..3044
                     /locus_tag="PWY67_002516"
     CDS             2442..3044
                     /locus_tag="PWY67_002516"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058732.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3138..3470
                     /locus_tag="PWY67_002517"
     CDS             3138..3470
                     /locus_tag="PWY67_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HDL2058733.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(3621..4600)
                     /locus_tag="PWY67_002518"
                     /pseudo
     CDS             complement(3621..4600)
                     /locus_tag="PWY67_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4605..5372)
                     /locus_tag="PWY67_002519"
     CDS             complement(4605..5372)
                     /locus_tag="PWY67_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2058734.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
BASE COUNT         1925 a          916 c         1001 g         1857 t
ORIGIN      
        1 attacaaatt agataataca ttagaagctg gagacaagat tgtctagata atgtgtgaga
       61 taaaattttt ggcaggttat catacataag acaaaatgtt tttaaaactg ataacgtatt
      121 tattccacaa caattacgca tttatttctg agaaaaatat aaagcaggag gattacaatg
      181 ctgaaaactt attccttaac aaaagataca ttattagttt cgtcagaatc aaatatcaaa
      241 aattcttctg ttgtttatgt ttatggtgac gaacgagaat atatcgaatc ttctgaacat
      301 aaatatgact ttaaaatagg caatatacta acgtttgatg atcgagttgc atatgacacg
      361 atgatagata agaacgatga aaaaagtttg ctcgtaagtt ttttgtttcc cgaggtacat
      421 gaaggtgggc atctagatac cctgacgaca tctgttgttt tcttggtatt taaaaataag
      481 ttgataattt ttacaaaaca aaaattaaaa ttcattcccg aactcttatc caatatggtt
      541 ttacgtgatg tcaatcattt cgtggaggaa gtacttttaa agatcatgca aaaagatttc
      601 tatatgatgc ttaacgacct tcgtggagtc aaggaaaaaa ttgatgattt agactcagaa
      661 atatcaacgt ccggttcact caaaccaacg tttggtgatt tattgactct tcaaaaatac
      721 atgatagctt tgtcaacaac ttataaagct aaccataaag cgcttgattt cattaaaaaa
      781 aatttcacaa ctattgacga tattgatttt agtactaaaa aaataattga tgatttatac
      841 gatacaagtg atacaatgga tagaatcatt ttaagttaca gccaatattt ggacaaattg
      901 gaaaatatga taaacaatat gatttcgtat cagctaaaca tgattatgaa aaccctaacc
      961 gagatatcaa ttgttttgac tattccagca attatttttg gcttttgggg aatcaatgtc
     1021 catgtcccgt ttgaaaagtc ttcttatggt gttttagccg tattgattat ctctgctata
     1081 ctaagtctaa tttgctggct tttgatgcga cgcaaaacat atttgtaaag tatttgtgat
     1141 accctaatat gatttaaagc agaatttctt tgttgacttt ttaaaaggct gattcctttc
     1201 aataatttaa tagcttcagc ggctgctcag aagtcaataa attggactat ttttattcag
     1261 ttacttaggc aacatttgag atacgattgc gattataaaa attgatatac caaccaatgg
     1321 ctgcttggaa gactgacata ttagcctcga tatcatcgaa taactaaata cagaaaaata
     1381 caataaatat tatcaaacta gggactatac tagaaaacat cagtacattc cttaactcag
     1441 ggtctataat tagaaaatcg cccctttgaa aactgattaa aacagccccc attatctagt
     1501 gactagataa tgggggctgt ttttttgtta agtgatataa taaaccataa aagaagttta
     1561 gaaatacggt cctggtcatc aataaataaa caatttaatt aattatttat ttaattattt
     1621 aatttcagaa aggtatacat gaatcatggt gcaacaaatt gtcctaccca tcaaagactc
     1681 cagcgtctta aagatggtac aagatacact tctcgatagt ttccgtgctg gtcgccgaaa
     1741 ttacaccgtt tttcaagttg gtaaggccac gctactacgt gtgagtgatg tcatgacatt
     1801 aaaaaaatca gatgtctata atccagacgg ctctgtcaaa aatgcaacct ttattcatga
     1861 taaaaagacc ggtaaagcaa acacgttata tttaaagcct gttcagcaag acttgttgca
     1921 atatcatgat tggctggccc aagaaaatat caattctgag tggttatttc cctcgacagc
     1981 ccattatgat cttcatatca ctgagaagca gtttcataag gtgatggcgc atgttggtga
     2041 tctcttagga atcaacttgg aagacaaaga gaaaaaattg cgagtcagaa aaccgttttg
     2101 agatatgaac agcatttgcc ttcacgcctt agcgatttta aataatagcc ttgaaagtga
     2161 ttatggaaaa gccctctacg ctcaatgcaa aattgacgta gaaactattt ttgatcgatt
     2221 gaaaggtgta tttggtatgt gaagaaccca tgtcagaggc aagaacgttg cacctactga
     2281 cattggaatg atgcttatga gcatgaatct aaccaaatta tcccttgagg ctagaagaaa
     2341 agcggaagct tttcaacaca aagcagccaa aaataaaaaa cgcgacgaag caatcacatt
     2401 tatgattatt catcgcgttt tttatattta gagctagtta tttgctagac ccactattta
     2461 cagatgaaac gtttatcacc tttggttttg ggcatttagt ggaattagca gaaccaggtc
     2521 attatgacga aaagtggcaa aattggaaac ttgaatcttt gccgattttt cctgatcgat
     2581 acgattttga agttgcaaaa gataagggaa agcagtttaa aattgttgca gaacttctca
     2641 aaaaggcaaa tacaattatt gttgcaacag atagcgacag agaaggtgaa aatatcgcct
     2701 ggtcgattat ccataatgca aatgcctttt caaaagataa aacatttaaa agactatgga
     2761 tcaatagctt agaaaaagat gtgatccgaa gcggttttca aaatttgcaa ccaggaatga
     2821 attactatcc cttttatcaa gaagcgcaaa cacgccaaat tgccgattgg ttgatcggca
     2881 tgaacgcaag ccctttgtat acgttaaatt tacaacagaa gggcgtacaa ggtacatttt
     2941 cactaggacg tgttcaaacg cccaccttat accttatttt tcagcgccag gaagccatag
     3001 agaattttaa aaaagaacct tttttcgagg tggaagctaa gtaaaacaag ggtcatttaa
     3061 gggcgttcta agccccacac agcgttttaa aacccaagag gagcttttta gcttttgttt
     3121 cttctaaaca agctaaaata ggcaatcaag aggggataat tgctgatgtt caaaccaaag
     3181 agaagaaaac gaatagtccg agttcgtttt ctttaagtag tttgcaatca aaagtcaatc
     3241 agctttataa agcgacagcg agccaaactt taaaagctat gcaaggactg tatgaagcaa
     3301 aattattgag ttatccaaga acagatacac catttattac agagaacgaa tttgcttatt
     3361 taaaagcgaa ttttggcaaa tatagcggtt ttttaggact tgatcttgaa atgttgttat
     3421 cattgataat gatacgttca aacaacagca cccaaatttt gacgaattag tgaaactcta
     3481 tgaaaaagac gtagtaaaac atgcaacatc ctattctaat cagctggtaa agcttaatta
     3541 ggttatataa gcttgaaaat aaagggtttg tccaatatgg acaagccttt ttcttatgtt
     3601 ttgccacaga tttgccacac ttattgtgta agagtatcaa ggataggtat aatttgatac
     3661 tttgtttttt tagtgatgtg caaataaatt tctgttacac gactacctct agaatgccct
     3721 acacggtctt gaattgcttc taggggtatt ccttactctg ctaagaaact aatatgcgta
     3781 tgacggaata tatgggtcgt aatatgttta ttaaaactaa gtttattatc aatatcaatt
     3841 cctgctcgtt ttggattacc ttttcgattt aattttccat tttcaggtga gttgaggaaa
     3901 ttgttaagga cactataatg aagaggtgtt ccattatagt cgggtttatg acgacttttg
     3961 gaagtaaaaa tccatccatt atctttataa tcaggattga atctcatatg ttggtgattt
     4021 aactcaattt cctctttaag aatttctata gcccttttag ataattggac atttcggtaa
     4081 ctttgtaagt tttttacatt accttcatag ctattttctg cagctcctcc gtgagtggac
     4141 tgactaatga tgatattttg cttttcaaaa tattgatagg caccaatatt ttggagtttc
     4201 gactctgtaa gtcaacaggg ccattagtta gaatacctat tgggatgcgg tttttatgta
     4261 gagccagcag tagttgatgt atctcaggaa tcatcgtaat ctgatcgagt tcatattcat
     4321 agtcagcttg gaagtctaga gcttctgtcc tagaaatact atcgatgcca aattcttcca
     4381 tagtcttctg acagcgaaaa attctaagct gctcaatact gatgagtttt ttcgtgtatt
     4441 taggaaaagc aacatcagac caatatctga aacgtttgta aatgttatct atttgttgga
     4501 tatcaatcgt tggaaaacat ttctcaacgc tacgtttaaa ggggagttga tggtcataaa
     4561 gagtgtcatc aaggtcaaaa acaatatttt tataagtcat aattctagtc cgcttttaaa
     4621 atagtagtgt actggctaaa ttcctctaag tgttgagggc taatctggtt atctgtaatg
     4681 atggtatcga gttgatctag attatagaag ttaaagaaat cgtatcgatc gaatttagta
     4741 ctgtctacta ataagaattt ttctacagca ttgtttaggg caagttgttg aatcacacct
     4801 tccttgtcac tatatgtagc aatagaatta tgggtaacag catttgcact aacaaaagct
     4861 ttggcaaatc taattgcttt taaatttgtc gaagccattg aacctacaaa agctccagtt
     4921 atttctctat attcaccgcc aagaagcaat aaatcaatgg tttcgctatc atttagaatc
     4981 aagaacacag ggagactatt tgtaatgaca cgaattttat aacctttacg acctttcaat
     5041 tctaatgcca gttgttcaag tgtagttcca ggtccaataa aaatagtatc gccatctttg
     5101 attaaagagc aggctttttt agcaatgtca attttttctt ttgtatttag acttttcttc
     5161 tcgatatgtg tcttttcaag tggcttttta gaggaaagca attgtgcacc accatgagta
     5221 cgtgttaaaa gtccatcagc ttctagagca tcaaggtctc tacgggctgt catatcggaa
     5281 atatctagtt catctattat ttcttttatg gttatggtcc catcaatctt taacatatct
     5341 aaaatttttt ctaatcgtcg ttttttgttc atatgaagac ccttctttca taaagtaatt
     5401 tttttccaaa gataattctc ttttaattgt atcataaaag acaatatttt caaggtaaaa
     5461 caaacaattt caaacaaaaa caaatgttag atgatgaaat aagaacagag gattgacgta
     5521 tattagctta ggtcagattt tgtataagac gaaaataaag taggacctct taatcagtaa
     5581 gttatagaaa gtaaaagact tttgtaatac ttgaatagat atttcacgtc cattttgtga
     5641 tggattaaat gaacaaaaat gaacaataat ttaacggtgt tatctatttt ttaaaaaaa
//
LOCUS       DANKAZ010000125         5692 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000125 DANKAZ010000000
VERSION     DANKAZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..599
                     /locus_tag="PWY67_002520"
     CDS             <1..599
                     /locus_tag="PWY67_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2058735.1"
                     /translation="NSYVRNMYGIFQMSLDRAVVLGLLEKNPAKVIGNVKKRKTTVDF
                     WTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVMRLGEATALTWKDIDFENKNLRVN
                     KTLYYKRAKTYEFVEPETASSIRTIELDDNTITFLKAWKERQVSIGGMSFVLSYNGTP
                     TQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLLISQ"
     gene            complement(883..1968)
                     /gene="xerS"
                     /locus_tag="PWY67_002521"
     CDS             complement(883..1968)
                     /gene="xerS"
                     /locus_tag="PWY67_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL2058736.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2302..2535
                     /locus_tag="PWY67_002522"
     CDS             2302..2535
                     /locus_tag="PWY67_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL2058737.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            2532..2939
                     /locus_tag="PWY67_002523"
     CDS             2532..2939
                     /locus_tag="PWY67_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2058738.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(4076..4594)
                     /locus_tag="PWY67_002524"
     CDS             complement(4076..4594)
                     /locus_tag="PWY67_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL2058739.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1905 a          916 c          908 g         1963 t
ORIGIN      
        1 aaaattctta tgtaagaaac atgtatggga tttttcagat gtcattagat cgagcagttg
       61 ttttgggact acttgaaaaa aatcctgcaa aagttattgg aaatgtgaag aaaaggaaaa
      121 ctacagttga tttttggaca aaagaagaat ttacaaaagt tatttcaact ttttatattg
      181 atgatttgta ccaacatttt ggttttattt gtatgtggtt actttttatg accgttatgc
      241 gtttgggaga agccactgca ttaacttgga aagatattga ttttgaaaat aaaaacttgc
      301 gtgtgaataa aacactctat tataagagag ccaaaaccta tgagtttgta gaacccgaaa
      361 cggcttctag tattcgtaca atcgaactag acgacaatac aattaccttt ttaaaagctt
      421 ggaaggagcg tcaagtttca ataggaggca tgtcgtttgt actttcttat aatggaacac
      481 ctactcagaa atatacaatc cgtcatatta tgagaagaca tgcaacgatc gttggtgttc
      541 atataatccg catacacgga ttaaggcata gtcatgcttc tttgcttata tcacaataaa
      601 aagatgcact tcgaattaaa gaaaggttaa aacatgctga tgtacaaacg actttaggaa
      661 cttatggtca tttgtatcta aattcattga gagagataac aaatgatccc acagattata
      721 tccaaactaa tttttctaaa atcaaaccga cacctattta cgataataaa aaataaatga
      781 taaacaaaag taggatcaca ccaaaagaaa cacactagaa aaactataat acaggctttt
      841 ctagcgtgtt gtttcttatt tccgcctatt tatacattat actcataaag catttaatgc
      901 atcgatcgta gccgcattgt ctatatgggt atataaagtt gttgtattgg tacttgaatg
      961 tcccaattgt tgggcagtta ataccaatga atttgtctgt tggtagagtc tagtagctaa
     1021 tgtatgacgc agtttgtgag ggctgacttg aattctaaag ccttttgaat attttgctac
     1081 catccgttcg attgcaatac cggatatacg cttggcttct ccttggtaaa ttgctagaaa
     1141 taaagctttt tcgttttctg ttactttata tcgttctttc cgaatagcta gataattagc
     1201 taaatattcc attgcgaatg ctgctatatt gacagcatct cttttatttc cttttctagt
     1261 tactaccaca ttattcttat ctaaagatag gtcatctaaa ttaatattta taacctcgga
     1321 gagtctcaat ccagatccta aaatcagtgc attgatcgcc aggtctcttt ctacatctcg
     1381 ttgataatag atcaaagctc gtttactcaa agtattttta tattcattgg caatataatc
     1441 tagataattt tgtgtatcct tatctagaaa taattttttc tcaatggcat tggcacgtgc
     1501 agctaacgta gttttatctg tgatggttgc tacttgatcc ataggattat tgtctaagta
     1561 tggttttcct agaggattat tggaactttt cgaaagatat ttaaatagaa cctttaatgc
     1621 cgtgatattt ctattgatag tagaagtaga tagacctaag ttattattat ctttgagctt
     1681 agtttttgtt gtctcttttt ttacattttc ttttgtgcga cgtttcaaat gagatttgta
     1741 caagtttagg tcataggcag ttaactgtct tatcgaggtg atagaagtat caggaacgat
     1801 agactcactc agaagccatt cataaaagag cttataattt tttagatatt ctaaagtggt
     1861 tcgaggtgat tgatctttat cctcacgatc ttgaacaaaa tcttttatat aatcgggcat
     1921 atgaagtatg attgtatcta ttttttgata tatttctgta tttttcatgt cattcatcct
     1981 ttcaataagt aggggagaag agtatctcta taattggact ataccaatta tagaatgttt
     2041 tgtcaattgg tatgctctaa aatttattaa tttgtttcaa aaaaagcata taaacaggta
     2101 tacgtttaat aatttaaaat aaatggtata cataaatagg gaaggaaata gaagggagca
     2161 atctaaattt tttgaatttt cttaattaat tatatagaaa gaacaatctt tctatagagt
     2221 tagacatgag agggactatg cttgatttaa aatcaacaag cgtatatact gtatatacaa
     2281 agaaaaagga gggagaagac tatggcaatt aaagaaagaa aatttaggga agtggggaac
     2341 tcagtagtat taactatttc aaaggagttt cttaaagaaa atgggttaaa tcccggagat
     2401 acggtttttt tagatgaaga aaaacttcga gcggcaatta caaaaaaaca agccttatct
     2461 gatgaaattg atttagcaat atctcaagca ttaactgaat atgatactac ttttagagag
     2521 cttgtcgata aatgagtatt cattacctag aagccaaaga tattataaaa atgaatgtaa
     2581 tccaaataaa aaaatactct cctaatgaac cccttggtgt aaaggatgcg aatgctttag
     2641 aaatgtgtgt agggcaactt cgagcctcag cttttggtga agaggtttat ccttcgattt
     2701 atgaaaaagc agctatccta cttatacaat tgataaagaa acatcctttt cacaatgcaa
     2761 ataaacgtac tgctttctta gctacttttg tctttttgaa aatcaatgga cagcttttag
     2821 ctattgatca aaaagatgct gtaaatttag cggtatatat tgctacgtat gataaggatt
     2881 tcgatcaact aaaatatgaa gtgatcaaca ctataaagac acatacgcag cccatttaag
     2941 gagttactgt atgtgtcttt atagggttat tagctgctat agcgaaataa ttgaagtaaa
     3001 ttacaaatag ctataattca aatccaacta tatagaaaac gtaaattttc tatatagttg
     3061 gattaagata acgactattt attttatcat atttttacta taccctatac ttttacttgg
     3121 tatatttatg aataatcgca aatttacccc tatactacta gtttgtaagt tagattaata
     3181 aaaatactaa agttatttgt tcttttatta aatacaccta caacttataa attgatggta
     3241 tttagataac acgaaagaat aaaaataaca gctgatagta aagtttgttg acgattgcta
     3301 ctcaatcgaa agtgagttag tgcgtatgaa cttgcttctg aaaacttatg aaatgaaaga
     3361 ccttttacat cttgcagaaa tcggtactgg gattaaacta agcaataaaa actaatgttc
     3421 ccctcattct ttgaacttct actatttgat ttatttattc tgataaatga cgatttcacc
     3481 ttcaggatct tcaaatcccg tatagaattt atttaagatc tcttcagtat cagtaatagg
     3541 ataggcgata ggccacatac cctttgatgg ttagaataaa ctgctcaaaa tctgtatcgt
     3601 atgttggctt tagcatatta acaaaggcta tctccaaatt ataaaccata agcgttgaat
     3661 cgttcttaac aatttcaatt cttcctaacc caaagtcaga agatataatt accggacttg
     3721 ccttaccctt catgtgggtt gttgagattc catatttaaa gggaattgtc ggtactatcg
     3781 gcggtctgaa aaacatccca aaaataaaaa aatctttccc ctgctagtct cctaagtccc
     3841 tttgtagcgg atatggcacc ataatctcat tttatcgaag tgagtcatgg tttatctgtt
     3901 ggccaaccaa aattgataca atctaacgat gcaccctcta cggcattcta ggggagtgca
     3961 cttgcattca cccaataaaa accggcagtt agcaaagaac ttatgtagtc gtatgttctt
     4021 actgttaaca tacaagaggt ctcctttcca caattgctta gcagtaaacg ggaatttaca
     4081 aagctaatct cttccatttt cttatctttg ttctaaaagt cccaaatgga gcaactgtat
     4141 ttacatgaat aaatttatat acttcccata cagccgtttt ggttgcctcg tctgcccatt
     4201 ttcttttgtg tgcagtgaat agatcttcat cactcattga atcaatcatc atgttaatat
     4261 catcaatatt acgattgaga atttcctcta attctttcaa tggtagatgt gcataggtat
     4321 ttgtaaacca cttatataac tctcctaact gattccattt aaattgatca gaaggcgttt
     4381 taacagataa tccatttctt tcatcatttt cccattttag aattaaattc gtccaaccaa
     4441 cttgatatgc taaattttca gcaggtgttc tatccacttc ctcaactctt ttatccttca
     4501 attcttcagg aatagtttca aattctaaaa tatatttagt ataagtccta tttatttctt
     4561 tttttaattc ttctttattt ttatatgttc tcattatatt acctccataa attcctaatt
     4621 gtctccttta attcatctat aatttcatcc acgatatttt aatccgttct acaatgcata
     4681 tctgatatgc attgactgtc taactcatat gtgaatgatt tataaaatat cccaacgtct
     4741 catctaaaga aatgttgatc gtcagcaagg agctaaaaaa tacgaatata tttcttttgt
     4801 agtaaaatcg gactggtttg catatcattt tcttcgtaaa tatcaaaatc atgttaacta
     4861 ctatcaatta ctaataaaaa ctaccacttt aagcgttaag aactgttaag tttagttaag
     4921 tgaggatagt tcacaaaatc cattgttaaa cttcacgaaa gtggtaaaac tcaaaatgct
     4981 ttatatagga ttacagcatc gctctttctt cgattgcgcg ttgggtaaaa aatattatga
     5041 agtaaaaatt gacgatgaca cgttccttac ggctcatcag atgaaacagc tacaaaaacg
     5101 aaatgatcaa ctcgaagaaa atttaattct aaaaaagcga ttgccatatt cacgcctcgc
     5161 tcgacgaact attaaaggtc aagatttacc tgaagatcat cctttttagg aaattgaagc
     5221 tttttatgtt gcacaatgca tttataatac tacaatgatg ttttcaccag atattcttct
     5281 agggtgcggt gtaatgaaac agatacactt gcaaaagaaa attcaccgcg aatttactaa
     5341 aattatgaat aattatgtta atcttccgaa atttttttcc aaaattaagg gatcaagctg
     5401 gtgttataga ttgtttaagg ttggcaaaaa aataacttct ttttaaacaa ttatagaaaa
     5461 ccgatactct gttaagttgg ttgacacggt gtcgattttt atgactttct tattctgaaa
     5521 cttcactaac ggacagtacc taaaaagtct atagtatgga cgtttacgag tactatttta
     5581 tcaaaaatgc tgctcatttc aacttatatg ttttagtgaa ctacccccac ttagcttgga
     5641 taggctacaa ttaactagac agaaaaatta aggtgtgtag actagaagaa aa
//
LOCUS       DANKAZ010000126         5615 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000126 DANKAZ010000000
VERSION     DANKAZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(60..2009)
                     /locus_tag="PWY67_002525"
     CDS             complement(60..2009)
                     /locus_tag="PWY67_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL2058740.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="PWY67_002526"
     CDS             complement(2051..3109)
                     /locus_tag="PWY67_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HDL2058741.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="PWY67_002527"
     CDS             complement(3228..3683)
                     /locus_tag="PWY67_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058742.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="PWY67_002528"
     CDS             complement(4267..5070)
                     /locus_tag="PWY67_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2058743.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2077 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatatttaat
     5461 aaaggaggat tactcctctc ttatttcaaa atcttaaaaa taaaaaatct taaaaaaaac
     5521 taacactatc taggagttta ttttgcttgt gtaactttga ataattgaaa aaaaggaaaa
     5581 ggaggaagct tcagcttccc cttttattta tattt
//
LOCUS       DANKAZ010000127         5532 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000127 DANKAZ010000000
VERSION     DANKAZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..985)
                     /locus_tag="PWY67_002529"
     CDS             complement(<1..985)
                     /locus_tag="PWY67_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HDL2058744.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="PWY67_002530"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="PWY67_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL2058745.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="PWY67_002531"
     CDS             1810..2964
                     /locus_tag="PWY67_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2058746.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="PWY67_002532"
     CDS             3179..4147
                     /locus_tag="PWY67_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL2058747.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="PWY67_002533"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="PWY67_002533"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL2058748.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5532
                     /locus_tag="PWY67_002534"
     CDS             5177..>5532
                     /locus_tag="PWY67_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HDL2058749.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRG"
BASE COUNT         1669 a         1078 c         1252 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc gg
//
LOCUS       DANKAZ010000128         5450 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000128 DANKAZ010000000
VERSION     DANKAZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            367..555
                     /locus_tag="PWY67_002535"
     CDS             367..555
                     /locus_tag="PWY67_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058750.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="PWY67_002536"
     CDS             545..805
                     /locus_tag="PWY67_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058751.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            1138..1710
                     /locus_tag="PWY67_002537"
     CDS             1138..1710
                     /locus_tag="PWY67_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058752.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="PWY67_002538"
     CDS             1711..2022
                     /locus_tag="PWY67_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2058753.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="PWY67_002539"
     CDS             2013..2273
                     /locus_tag="PWY67_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058754.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="PWY67_002540"
     CDS             2434..2793
                     /locus_tag="PWY67_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058755.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="PWY67_002541"
     CDS             2790..4433
                     /locus_tag="PWY67_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2058756.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..>5450
                     /locus_tag="PWY67_002542"
     CDS             4468..>5450
                     /locus_tag="PWY67_002542"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058757.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKR"
BASE COUNT         1944 a          751 c         1134 g         1621 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca
//
LOCUS       DANKAZ010000129         5423 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000129 DANKAZ010000000
VERSION     DANKAZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            131..505
                     /locus_tag="PWY67_002543"
     CDS             131..505
                     /locus_tag="PWY67_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2058758.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="PWY67_002544"
     CDS             complement(619..1248)
                     /locus_tag="PWY67_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2058759.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="PWY67_002545"
     tRNA            complement(1382..1454)
                     /locus_tag="PWY67_002545"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="PWY67_002546"
     tRNA            1668..1741
                     /locus_tag="PWY67_002546"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="PWY67_002547"
     CDS             complement(1879..2043)
                     /locus_tag="PWY67_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058760.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="PWY67_002548"
     CDS             2338..3666
                     /locus_tag="PWY67_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL2058761.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="PWY67_002549"
     CDS             3868..4701
                     /locus_tag="PWY67_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL2058762.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..>5423
                     /locus_tag="PWY67_002550"
     CDS             4717..>5423
                     /locus_tag="PWY67_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL2058763.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLY"
BASE COUNT         1824 a          838 c         1071 g         1690 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttg
//
LOCUS       DANKAZ010000130         5422 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000130 DANKAZ010000000
VERSION     DANKAZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(108..770)
                     /locus_tag="PWY67_002551"
     CDS             complement(108..770)
                     /locus_tag="PWY67_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2058764.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(780..1595)
                     /locus_tag="PWY67_002552"
     CDS             complement(780..1595)
                     /locus_tag="PWY67_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2058765.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1856..2299)
                     /locus_tag="PWY67_002553"
     CDS             complement(1856..2299)
                     /locus_tag="PWY67_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDL2058766.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2433..2771)
                     /locus_tag="PWY67_002554"
                     /pseudo
     CDS             complement(2433..2771)
                     /locus_tag="PWY67_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(2759..3136)
                     /locus_tag="PWY67_002555"
     CDS             complement(2759..3136)
                     /locus_tag="PWY67_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2058767.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3360..4553
                     /locus_tag="PWY67_002556"
     CDS             3360..4553
                     /locus_tag="PWY67_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2058768.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4719..5141)
                     /locus_tag="PWY67_002557"
     CDS             complement(4719..5141)
                     /locus_tag="PWY67_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2058769.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         1665 a         1018 c          914 g         1825 t
ORIGIN      
        1 cgttttattt catttgttta gccatatgtt tcaaaaaatg aaaaagaatg acagttttta
       61 cataaaaaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
      121 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
      181 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
      241 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
      301 tttggtttcg acgctggatt tctcacctga aaataccagc agttggtgct aaaggaaact
      361 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
      421 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
      481 aatcccttta gccaaaattg gcgaaacatc tttatgaaca aacgtactgg tgaaaaacta
      541 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
      601 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
      661 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
      721 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
      781 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
      841 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
      901 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
      961 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     1021 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     1081 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta atattgcttc
     1141 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatct
     1201 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     1261 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     1321 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     1381 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     1441 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     1501 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     1561 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     1621 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     1681 ccagaacaac ggttattata gctgattcat ttccgttctt cttatttttg taagcgctta
     1741 tttaacaagt tatgtaaatc tctttatttt tttgatttaa aaatgaaaaa atacaaaagc
     1801 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     1861 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     1921 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     1981 ctcgctttga atgatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     2041 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     2101 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     2161 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     2221 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     2281 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     2341 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     2401 tttctagtaa tagtgccaat gtattcgaac tatcagcttc cacacagccg atcgtaaatc
     2461 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     2521 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     2581 cggttttcgc gtacaaacag ttctgttcct agttactctt ctaactcctt cagttgtcgg
     2641 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     2701 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     2761 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     2821 gtaaacgttc caaacattct tgcatttcag caattttttt cgtcaaaagt tcactttctt
     2881 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     2941 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     3001 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     3061 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     3121 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     3181 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     3241 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     3301 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     3361 tgacaacgta tcagcgaaag gtattagtat cgacatcggc gggaattgct ttagagaata
     3421 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     3481 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     3541 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     3601 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     3661 ttcgctttat cgcaggtgta ggagcgggag gagaatatgg ggcgtgtatg tccttgattt
     3721 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     3781 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     3841 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     3901 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     3961 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     4021 tccaaattgc cggctatttt gggttaatga attggctacc ttcaattatg caaaatcgat
     4081 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     4141 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     4201 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     4261 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     4321 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcgtta
     4381 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     4441 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     4501 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     4561 gtctgtaaat gacaagaaag aggtgtggaa aaagcttgca tctgcggtaa taagaccagc
     4621 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     4681 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     4741 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     4801 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     4861 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     4921 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatattgt
     4981 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     5041 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     5101 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     5161 actttactat actacataca agaaaaaaac ggatacaaat atcatattgt cacaccgtca
     5221 atagtgcata aacctgttat tatttctctt tttttcctaa gtattctatt gatatgaatc
     5281 atttaatcgt ttattatata aatgtcaata atctaataat aaaataacgt acatatttta
     5341 ttattgcata cttaataaca gaactatagc aaaaaatccc gtgtatcaac acgggattag
     5401 attaagtttc ctcaatcact at
//
LOCUS       DANKAZ010000131         5376 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000131 DANKAZ010000000
VERSION     DANKAZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(32..253)
                     /locus_tag="PWY67_002558"
     CDS             complement(32..253)
                     /locus_tag="PWY67_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058770.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(250..540)
                     /locus_tag="PWY67_002559"
     CDS             complement(250..540)
                     /locus_tag="PWY67_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058771.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(537..1721)
                     /locus_tag="PWY67_002560"
     CDS             complement(537..1721)
                     /locus_tag="PWY67_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2058772.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1903..3306)
                     /locus_tag="PWY67_002561"
     CDS             complement(1903..3306)
                     /locus_tag="PWY67_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2058773.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3328..4101)
                     /locus_tag="PWY67_002562"
     CDS             complement(3328..4101)
                     /locus_tag="PWY67_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058774.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4111..4488)
                     /locus_tag="PWY67_002563"
     CDS             complement(4111..4488)
                     /locus_tag="PWY67_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2058775.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4509..4823)
                     /locus_tag="PWY67_002564"
     CDS             complement(4509..4823)
                     /locus_tag="PWY67_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2058776.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(5023..>5376)
                     /locus_tag="PWY67_002565"
     CDS             complement(5023..>5376)
                     /locus_tag="PWY67_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HDL2058777.1"
                     /translation="EVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTV
                     IAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLL
                     YPTQLLDKIEFDYNI"
BASE COUNT         1577 a         1070 c          919 g         1810 t
ORIGIN      
        1 atacttccta ttttatcatg aaaaatatct gttaagcacc aatgattcgg ttaaataagt
       61 ttaataacac atctttcacg ccagctgcat tgaacacaag tccgacggcg attagagcaa
      121 taatcaagaa gccaatgagt tttgaaaatt ctcgtttaaa acctaaaaac agtccaatga
      181 caacaatcgc taacaatacc aatgactgtg cattatccaa gaaccagttg tacaaattct
      241 gcccaaaatt catgtgattg tcctcctttc cttaagatat ttttctcgat aatagatata
      301 gctttttaca cgccttaata attgaaaagg ggttaaatgc tctccataga cggttactcg
      361 attggtataa ttattataaa atctcacctt gtaaaaatca taggtataga tggtatcacc
      421 agcttgccgt tttccctcaa taatgagaga cgtatatttc cctgtatttt gtatatacgc
      481 ttgaaactca atgccacact gaaaccctaa cttctttaat tcttttgtta aaaggctcat
      541 gtaatcaaat cctccatggc tagatgttgt tgttcaatta atttctcatg tttttctgtc
      601 agtttggctt cctgaatcat ggtggagata atggttgtac cgttgagaac atctaaaatg
      661 cttgcgattt ttaatgtagg ggcgacttga tgatgtaacc agttcaatgt tcgttcaaaa
      721 gaataaggct ctggctgtgt ggttaggcgt aatgctccac gatttttacc gataaaccat
      781 tcccaacgtt cattagtttt ccaatcactc cgacgtttag tgtcgtcttt atctgcaaag
      841 cggatatagc gattgataat ttcaaaggct gttttttccg cctgttgatg tgccaataaa
      901 tctttcatgg cttgggtggc tcggtcattt tttaaccgaa tttcaaaccg attctttact
      961 tctgcgtctt ctaagggaac atcatttttc acgtattgct cataatcttt ctcatatata
     1021 cagaaataga cttcactttt taatgagcca atataaagtg tgtttcccat gtatggtttt
     1081 tcatcacggt gaaccaattc accactgcga taatttttaa agcttcgaaa gacggaaatg
     1141 cattcttcct gtttacattt ctttgccaaa tctggaatat tcaaaatccc tgttttatcg
     1201 ttgatagcta aatccaagcg tttgaaaata cctttatgtt ccatgcaatc ttgaaagaaa
     1261 tcataccagc tccgatgttg agccaataga aagttttcaa attgccgaca gccttttcct
     1321 ttcagttcta ataaaacacc tttacttaca tcatgagaga ctaggacaac aatatcaccg
     1381 aaacgatagt gttcgggata agaataatag ccaaagtctt cgtgtagcat ataatccata
     1441 ttcaaacgta aaacctcacc gataatttga gccacatcat tggtagggaa tcgaatccgt
     1501 acatagtcga ataacatctc caatgggtta tcgggattga agcgttccaa agcgttcagt
     1561 aaatcttctt tcacctgttg agtagggact gccttgcctg tttcaatatc cgataggtaa
     1621 ggtctggtaa tatgagaagc aaccgccagt ttattttgag aaacgccata agccagtcgc
     1681 ttttctttca attccttaat ccaaattttt tcgttttcca ttcgcaaacc tccaatctaa
     1741 aaaagatgta aactatgacc cttgagttta cacctttcat attttccgca atcctttgta
     1801 ccatcaacat tggttgatga gaattgcctg ttttcagttg tatttgtgcc cccctgttag
     1861 atagaggggg ctaaaggatt ggcgtggcta acgccacacc aatcaaagca agtctgaacc
     1921 tgtacctttc acttcgtgcg gtacaggctc agattgcttt ttttgaatga cttttcctat
     1981 ttctcctaag aaatcgtacc tttttggtac aaggggtgta taaaactcac taatcacact
     2041 ttttcctgta tccacgtaac ctcttccctt gatgggtttt aagaaaaatt gtttttgtgt
     2101 atcgcttccg aacatcatac caaagcccat ttcagacata cgacccaaag ccacacggaa
     2161 gttaaagttg tcccgaatcc catcactaaa atattttgcg tctggacgtt gacaggcaag
     2221 aattaaaaag aagcctgctt gtcgtcctaa cattacaatt tgtttgagtt tatgaataac
     2281 ggtggtactt tcacgaccta gcatatccat cattgccaca tattcatcaa aaataagaaa
     2341 attggcagac aaaccaagat aagcataatt ttctcctgtt ttatagttag ggtgttgttt
     2401 catggtttca ctgcgatcaa tcatatcttc gtaaaattga ttgatacatg ccaacatatc
     2461 ttctttacgg taatagacat tattcatgac cgcccctaag tctgccaaat cagcattttt
     2521 agggtcaaga atataaagct ttgcatttgt atgcaggaga gattcaatca atgtgagaat
     2581 aaaataggat tttccactac cagtgccccc agcaattaac atgtgaggga gagaatcgaa
     2641 ctcccaatag accgattcca tcaatttgac acgtccatct tgagctacca cgtcttcaat
     2701 gccaattcga ttgccaatag tatcataaag taggacatac tctacataag aatctttcaa
     2761 cgttttttcc actaattcac aatacaatcc tgtttctagt ttgttttcta ggtgcaaaag
     2821 ctgatcttga tatttcccta aggtgatttc tacatgaata tggattagtc catctttcat
     2881 tcgataatat attttaggaa aatggcggat tcgttgtttg gttttcttgc tgggtaaatc
     2941 tttaaagaat ccctctgatt gagtttcttc ggcttcatac caatgatttt ctaaaatcat
     3001 acgagctaac ttttgacggt gctctaactg ttttatagaa tctatacgat agtgataata
     3061 aagccagctg ataagtccgc atacaaaaag agcagtgaga atactgctcc aaaagtatgg
     3121 cgtatgaagt tgatagaaaa atgtatctaa ctgaactgct tgccagttca ccgattgtaa
     3181 cgctctccaa tgaaaaggga gtaggacgac tagaaaaact accagcaatg aaattagtac
     3241 aaaatgatag accaagtgct tatcgttggc tttgattcgc tctcctctgt catgaagcaa
     3301 tggcataaaa atcctccttt ccacctatca aaataattgt gtattgtaga tacgagcctg
     3361 tttatcaaaa gttggttgag aaatggcttc atatttctga taagcgattc gcttttcaaa
     3421 gcgtaaattt ttttctttcg gataataatg acgtcgatag agagtgtgtc ctaacagttt
     3481 tttcgcttct tttgtcaacg gatagagata gcgaaacatg cgtccattga ttttctcaat
     3541 ccctttgtat tcgcaaaatg cctgggaaag ccaatgcttt ttctctacct gttcaaagcg
     3601 agcattttcc tctaataaaa ggcgagcact tcgagggtgt atcttttccc atgactgctt
     3661 atcacgatag acacttgtct tgaaataccc gcagtaaaag aagttggacg cttgatagac
     3721 atagccacat tttccctcaa ttccatctgc cagtgtatat aaaaaatgac agtccgtatg
     3781 ttctttcaac cacttaatta gagctgaaag agcttgactg ccaaaataat tggtttgatt
     3841 catttctgga agaaaacaca tctttccaat ttctaaataa tctgtggttt gaagggaact
     3901 gtcgggaaag agtttgcgta tggtttgtag gggttgcgta ccccagccta attccacaac
     3961 gcctaataat ttttcttctg aaaaaatccc tagaaagtat ttacacaatc tagggagtat
     4021 ctttgaataa tggtattgcc gaataaaaga aatggcttca tccttcggaa tttctttcaa
     4081 aatcaatggt gtgtttgtca taagcaacac ctatttttca ctttcggacg cttctttttt
     4141 atatttctga tgaggttttg tctgtccttt taatacaaga tcgtctgctt tcacgtacca
     4201 atcaacgtct gcccctcgaa aagtagccgt cgctacggta tctgccacag gattgattaa
     4261 ttccacttct gcgttgtagt caaattcttt caaaggaaca gaagcgggga tactgacttg
     4321 tatcatgcgt ccttgtcctt ttgattttaa gtcataggta cgttctttaa tttcttcgga
     4381 aaccgtacca tcttcattct ggatacgaac ctcacgacgc aaggcggaaa atttcaatgt
     4441 gccaaatgtc tggtcttttt caatcacaat accttctgct aatctcatat tttttcacct
     4501 ttcctttctt atgctttcac catatcgtct gcgtgcataa tgtaattggt aaatcctcga
     4561 ttgccaattt tataaccttc tgcggtaata tgtgggttga tgagtttgac tttttcgtcg
     4621 gattcaaaat gtttctcgcc agcttcggag ggaagaatga caacaatatc atctgctcgt
     4681 tgaatcgttg agtataaatt gtaactacga gagagaatgg ttggtctgcc atttattcga
     4741 cgttgttcaa tgttcccttc accagcatat tctaattgtc caaatgtttt ttccatattg
     4801 ggaatgacat atttaagttc catattttta cctatccttt ctgtttttat gtttgaatga
     4861 atcttatttg tgtgatggtt ttaaaggggg gagagcgacc ttttgattct tgatttttta
     4921 ttttcataag ttcacttcct ttcaaaattg ggtaaaaaaa tagacgctcc gtactgagcg
     4981 tccgattcct actatattta aaacaacgac tacctaggta ttttaaatat tataatcaaa
     5041 ttctatttta tctaataatt gagtgggata aagcaagtct cctttactcg taaaataacc
     5101 cacctctgag aaaagaaata aattattttt tgctcttgaa caaattacgt atagtagttt
     5161 tctcgctgaa ctaattcctt tatcagaaac attaaaaatt tcattccaat gaggaatttt
     5221 cccgtttaga agattaatag cgataacagt atcatactca tttcctttaa ctttatgtgc
     5281 tgtagtaagt gtgataccat ctcgagattt aaacatcttc ttaaaactag atacactact
     5341 agataatgaa ttatttttta tccgatcttc tacttc
//
LOCUS       DANKAZ010000132         5375 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000132 DANKAZ010000000
VERSION     DANKAZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            8..1015
                     /locus_tag="PWY67_002566"
     CDS             8..1015
                     /locus_tag="PWY67_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2058778.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1027..2031
                     /gene="neuB"
                     /locus_tag="PWY67_002567"
     CDS             1027..2031
                     /gene="neuB"
                     /locus_tag="PWY67_002567"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL2058779.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2028..3176
                     /gene="neuC"
                     /locus_tag="PWY67_002568"
     CDS             2028..3176
                     /gene="neuC"
                     /locus_tag="PWY67_002568"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL2058780.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3149..3826
                     /locus_tag="PWY67_002569"
     CDS             3149..3826
                     /locus_tag="PWY67_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2058781.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3816..4958
                     /locus_tag="PWY67_002570"
     CDS             3816..4958
                     /locus_tag="PWY67_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL2058782.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            4971..>5375
                     /locus_tag="PWY67_002571"
     CDS             4971..>5375
                     /locus_tag="PWY67_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2058783.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHN"
BASE COUNT         2002 a          691 c         1047 g         1635 t
ORIGIN      
        1 gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt ccattggaag
       61 tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt atagtagagg
      121 tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt tgagattttt
      181 aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg tggatattat
      241 ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta tggaagctgt
      301 aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa ataatgtaaa
      361 acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca tgggtgcgac
      421 aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa aaacagaaac
      481 aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg ttattcctct
      541 ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg atatgacacg
      601 atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta tgaattcaaa
      661 atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt tagctcaatc
      721 attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa taaaagttgt
      781 aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg aaattgaacg
      841 ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta ctgacgaact
      901 gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa cagaattaat
      961 gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt ggtaaggagt
     1021 tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca taatggaagt
     1081 ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa cgcggttaaa
     1141 tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc aaaatatcag
     1201 aaggataatc ttaaagagga cagttctcaa tttgaaatgc taaaaaaatt agaattatct
     1261 caatctgatt ttattagaat aaaagattat tgtatacaaa ataaaattga ttttttgtct
     1321 actccttttg atgaagaaag tgcagacttt ctaaaacaaa ttggtgtaca aggatttaaa
     1381 attggctcag gagatttaac aaatttacct tttcttagaa aaatagatat gtatgggttg
     1441 ccagttttat tatctacagg tatggctgat attcatgagg tcagtgaggc tgtagattgt
     1501 ttcatagata gtccagttac tattttacat tgtacttcaa attatccggc tagttctgaa
     1561 gatattaatt tattggcgat taacacatta aaagaaatgt tcgggatacc cgttgggtac
     1621 tccgatcatt cattgggata tgatgttggt atttgtgctg ttgcgatggg tgctaaggta
     1681 attgagaaac attttacctt ggataaggaa ctacctggtc cagatcataa agcttcttta
     1741 gatcctcagg aatttgcaga ttttgtaaag catattagaa acactgaaat atttttaggt
     1801 gatggtgtta agcgagcaat gccttctgaa cttagtacaa aagaagtatc aagaaaaagc
     1861 ctagtggtca ctgagaattt aaatgtggga gatattctta cagagaaatc tcttacaata
     1921 aaacgtcctg gaaatggaat agctcctaag tttttatatc aatatattgg caaaaaagtt
     1981 aaaagaccaa taagtgcaga tagtgttctg cttgaggaag atgttgaatg aaaaaaatac
     2041 ttatagtaag tggaacacga gctgattatg gaatatataa acctgttgct gagaatttac
     2101 tacaagataa ttgggatgta tcatttgttg tttctggaat gcatttatcc cataaatatg
     2161 gatttactaa agaattgatc ttagctgacg gatttagaat tagtggagaa gttcaaagtt
     2221 tgtttttaga gaacaataag ggaaacatgg ctcggtctgt ggcattagag ataaatggtt
     2281 tcacaaatat atttgaacaa actagccctg attttgtatt attactaggg gatagaggag
     2341 aaatgttggc agctgcgata gcttgtttgt atcttggaat caaaacagca catattcacg
     2401 gaggcgaagt gtctggtact gtagatgaat caataagaca tgccataacg aaaatagcat
     2461 ctgttcattt tgctgctacg gaaaaaagca aagagcgttt aataaaaatg ggggaagatt
     2521 cttggagagt ttttcccgtc ggagctccta gaatagatac tattttaaat aatagattac
     2581 ctagttttag agatataaaa gaaaaatatc atctaaaatt tgaaaagaaa aaatacgttt
     2641 tgcaagtatt tcatccagtt gtaaccgaac tagaaaatat agaaaaacaa attttagctt
     2701 tgatcaattc aatggaacaa caatctcaac caattatttg cattttacct aatagtgatg
     2761 ctggtagcga aattatcaga aaaatgtacc agaaatattt aaaaacggat attatttata
     2821 ttacgaattt agagccggag gaatacctga ctatattaaa agagtgcaaa tttatgatcg
     2881 gaaattcttc ttctgggata attgaagctg catcattcaa aataccagta ataaatttag
     2941 gaaccagaca aaatggacga gaacaatcta aaaatattat aaatgcaaat ctaaatgaaa
     3001 atgaaattga aactgctttg aataaaatac aagatcctca gtttataaaa agcttgaaaa
     3061 atgttaagaa catctacggt gacggcaaca gttctagaag aataacaaaa acattgaatc
     3121 aaatattgaa tgatggggaa gatattaagt ggattcaaaa acaaatagct tattaaaata
     3181 tactagtgat ccatacatta ttattggggc tggcggacac gcaaaagtca ttattgaaac
     3241 cttaagatta gaaaataagt taatatatgg attttttgat gataagcaaa ataaaatgca
     3301 agatctgaaa tatcttggga aaataaacga attgaatagc tgggcggata gcaaaaaaaa
     3361 aatctataaa tttattgtag ctattggtga ctgtgtagta agaaaaaaga ttataaaaaa
     3421 attgaatatg ccaagtgagc aatatggaat agctgttcat ccaagtgcta tagtggcaaa
     3481 agaaatttca attggttatg gaactgttat ttttgcgaat gctgtaatta acccagatgc
     3541 tgtaattgga gagcatgcta taataaacac aggtagcatt gttgagcatg attgtagaat
     3601 taataactat gttcatttat ctccaggtgt atgcttagcg ggtggagtac atgtaggtgt
     3661 aggaactcaa ataggaattg gttctcaatg tatccagatg aagaaaattg gatcttggtg
     3721 tatgataggc gcagggagca ctattgttaa ggatattccc tcacattcct tagcttatgg
     3781 caatccagct aaaattaaga aagaaggaat taattttgaa ttctagaatt ttattatcaa
     3841 gtcctcatat gggaggaaca gaagaaaaat atgttgctaa ggcttttaaa gataattgga
     3901 tagcgccatt aggtgaaaat gtggataaat ttgaagaaag tattacaaaa tatactggtt
     3961 ctaagggcgg agcttctgcg ttaagttcag gtacagctgc catacatctt gccttaagac
     4021 tgcttaatgt tggaaaaaac gatattgtct ttgctccttc atttactttt atagctacag
     4081 taaatcctat tttatatcaa ggggctgtac cagtgttcat tgactcagaa cctaatacat
     4141 ggaatatgag ccctgttgca ctagagagcg cattagaaga agctgaaaga aacaaccaat
     4201 tacctaaggc aatcatcgta gtgaatttat atggtcaaat ggctgattat gaacgaattt
     4261 tagctattgc aaaccaattt aatgttccag tgatagaaga tgcagcggaa tcgttaggtt
     4321 ctacttataa gggaatttcg tcaggcttat ttgggacaat tgggatctat tcatttaacg
     4381 gtaataagat catcacaacc tcaggtggag gagctctagt gtctagtgat aaaaaatatg
     4441 ttgagcattc aagattttta gcaacacaag caagagatca agcaccgtat tatcaacata
     4501 gcgaaatagg ttataattac cgtctaagca atatttctgc cggtatcgga cgtggacaaa
     4561 tggaagtatt gaatgataga gtagctaaac gaagagaaat tttcaatcga tataaggaag
     4621 ctttttctgg aattgataga attgaaatga tgccagaagt ggatggcatg gtgccaaatc
     4681 gttggttgtc tactttaacg atcaatgatt ataattctat tgatcaagta gttaacgtaa
     4741 taaataaact aaatgaaaaa aacattgaaa caagagcttt gtggaaacca ctacatttgc
     4801 aaccagtatt tcaaggggta aaaatgtatt accatgataa taatcttagt gtgtcagaaa
     4861 agttatttaa tagcggctta tgcttacctt ctggaagtaa tatgacagat agtgaacaag
     4921 aatttgttat tgatgagttt aaaaaaatat tcaagtaggt gtgatatttt atgaacatag
     4981 gaattattgg tttaggttcc atggggaaaa gaagacttag actcattcaa gaacacttta
     5041 atgatatttt actatgtggt gttgatacta atgaggcgag acgaaaggaa gttgaaaagg
     5101 aatttggaat accaacttat aatagtctaa cagaggcaaa aaatttgttt gaaataaatt
     5161 cagtttttgt ttgtacatcc cctataaccc atgaatttat tataaaagag gctttaaatt
     5221 tagattgtaa tatctttacg gaaatcaatt tattaaatga atattatgat gaaataatcg
     5281 aagtagctaa aaagaaagat agattacttt atctatcttc tacatttttg aagagaaaag
     5341 aaattcaata tattaagtct aaagtgacgc ataat
//
LOCUS       DANKAZ010000133         5247 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000133 DANKAZ010000000
VERSION     DANKAZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..1389
                     /gene="gadC"
                     /locus_tag="PWY67_002572"
     CDS             <1..1389
                     /gene="gadC"
                     /locus_tag="PWY67_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HDL2058784.1"
                     /translation="GGILWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAI
                     FFQWFQITVGFITMIYFILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTN
                     RTAKIAKAGFVIGIVIPSIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFV
                     SFILAYMGVEASASHINELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLS
                     LSAGVIQTFETLILHFNSHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGL
                     LPVFFRKTNKQDVPVPLIMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGY
                     LLFFLGYFVLVFKKKDLKRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAAS
                     EHRTYQIILLISFVVTVLLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEH
                     HVTKNDEHILKH"
     gene            1402..2802
                     /locus_tag="PWY67_002573"
     CDS             1402..2802
                     /locus_tag="PWY67_002573"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDL2058785.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(2967..4407)
                     /locus_tag="PWY67_002574"
                     /pseudo
     CDS             complement(2967..4407)
                     /locus_tag="PWY67_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            4800..5247
                     /locus_tag="PWY67_002575"
                     /pseudo
     CDS             4800..5247
                     /locus_tag="PWY67_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT         1675 a          850 c         1001 g         1721 t
ORIGIN      
        1 ggaggtattt tatggtttct accagttgct ttatgtgctg cagaaatggc aacagttgaa
       61 ggatggaaag atggtgggat tttcagttgg gttagtcaaa ctttaggaga acgttttggt
      121 tttgcagcta tttttttcca atggtttcaa attacagtag gttttattac aatgatctac
      181 ttcatactag gagcgctgtc gtatgtaatt aattttccag agcttaatac agatccattt
      241 atcaagttta ttggtgtatt agtcattttc tgggtactga ctttttcaca gttgggcggg
      301 accaatcgta ccgctaaaat tgctaaagca ggatttgtca ttggaattgt tattccttcg
      361 atcattctct ttggtttagc agcagcctac tttatcggcg gtaatccgat tcaaatggct
      421 attaatgcga aagcttttat tcctgacttt tctcagcttt caactttagt agtatttgta
      481 tcttttatat tggcttatat gggcgtagaa gcttcagcat cacatattaa tgagctggaa
      541 aatccccaaa gaaactaccc attggcaatg tttttactag tgattttggc catcgcctta
      601 gatgcaatcg gcggtttctc agtagcagcg gttattccac aacatgactt gtctctcagt
      661 gctggtgtaa tccaaacatt tgaaacgctt attttacatt ttaacagtca tttacattgg
      721 ttggtaaaag tgattgctct catgattgcc tgtggcgtta tgggtgaagt tagttcttgg
      781 gtagttggtc catcacgagg gatgtttgca gctgctcagc gaggtctgtt accagttttc
      841 ttccggaaaa ctaataaaca agacgttcca gttccgttga ttatgataca aggaattatc
      901 gttactattt gggcagcagt cttaactttt ggtggaggag gcaacaattt atcattctta
      961 gtagcaatct ccttaactgt tgtcatttat ctcgtaggat atttactatt tttcctaggt
     1021 tactttgtat tagtttttaa gaaaaaagac ttaaaacgaa cttacaatgt accaggaaaa
     1081 actgttggca aaacgattat cggtggaatt ggttttattt tatcagtgtt cgcattgctt
     1141 atctcttttg taccaccagc ttcaattgca gctagtgagc accgaacata ccaaatcata
     1201 ctacttatta gctttgtagt gactgttctt ttacctttta tcatttatga agtacatgat
     1261 aaaagtgctc acacaactat tgaggaacct actcacgtga aagctgaaga cgttaatccc
     1321 gctgtttatc cagctgctag aggagagcac cacgttacaa aaaatgatga acatatttta
     1381 aaacattagg aggaattcat tatgttatac ggaaaagata atcaagaaga aaaaaactat
     1441 ttggaaccaa tttttggctc tgcaagtgag gatgttgact tgcctaaata taagttaaac
     1501 aaagaatcca ttgaaccacg aattgcttat caattagtac aagacgagat gttggatgaa
     1561 ggaaatgcgc gattaaactt agctactttt tgtcaaacgt atatggaacc tgaagcagtg
     1621 aaattgatga cccaaacgtt agaaaaaaat gcaattgata aatcagaata cccacgaaca
     1681 acggaaattg aaaaccgctg tgtaaatatg attgctgatt tatggcatgc tccaaataat
     1741 gaaaaattca tgggaacttc aacgatcggc tcttcagaag cctgcatgct gggtggtatg
     1801 gccatgaaat ttgcttggcg taaacgtgct gaaaaattag gtcttgatat tcaagcaaaa
     1861 aaacctaacc tggtgatctc ttctggttac caagtttgtt gggaaaaatt ctgtgtatat
     1921 tgggatgtgg aactgagaga agtcccaatg gatgaaaaac atatgtcaat taatctagat
     1981 actgtcatgg attatgtgga tgagtacaca attggtattg taggtattat gggtattact
     2041 tacactggtc gttatgatga tatcaagggt ctgaatgatt tagttgaagc tcacaataaa
     2101 caaactgact ataaagtata cattcatgtt gacgctgcat cgggtggctt ttatgcacca
     2161 tttactgaac ctgatctagt ttgggatttt caattgaaaa atgttatctc aattaattct
     2221 tcaggtcaca aatatggttt ggtatatcca ggtgtgggtt gggtcttatg gcgtgaccaa
     2281 caatacttac cagaagaatt agtatttaaa gttagttact taggtggaga aatgccaact
     2341 atggctatca acttctctca tagtgcagca caactaattg ggcaatacta caactttgta
     2401 cgctatggct ttgatggtta tcgtgatatt caccaaagaa ctcatgatgt tgctgtttat
     2461 ttagccaaag agatcgaaaa aactggtatt tttgaaatca ttaatgatgg atcagaattg
     2521 ccagttgtgt gctataagct gaaagaagat cccaatcgcg aatggacact atatgattta
     2581 tctgatcgtc tgttaatgaa gggatggcaa gtcccagcct acccattacc taaagacttg
     2641 gatcaattaa ttattcaacg cttagttgtt cgagcagact ttggaatgaa catggctggt
     2701 gattatgtac aagatatgaa ccaagcaatt gaagagttga ataaagctca tattgtttat
     2761 cataaaaaac aggatgtgaa aacgtacggc tttactcact aaaattcttc agagaaatat
     2821 aaaagaagtt aattaactaa taaaaggagt tgtgattacc acacagctcc ttaaatctat
     2881 ttgaataaat atatgatatt catttgttac actctcaatc ttagctgttg ttctattcat
     2941 cattactctt attgtaagta gttcagtcaa atttgcttgt tcaattgatc gataacagta
     3001 tgaattgttt gaaaagtaat cggatatata attgttttct cactacctaa gccttcttgg
     3061 tataaagaac tatcgcaaag tataatccgc tgagtacaaa agaaagtgtc gtctactatt
     3121 tctctaagtt gtttacaaat gatattacta atagaaaagt gttctcccaa aatgtcttct
     3181 aactgttgtc gatagtataa atacctaaag tctgacggag ctactatgaa cacttcaatt
     3241 ggagatttcc gtttatgctc atttaaaaga cttaaactaa tggttagttt acgaatagaa
     3301 ttctcattcc aacgcagaaa taaattgttt tgttttcgcc attgttcaag aatttcatga
     3361 acttctaagt acaaagcgcg ttgctcttca tttaacaagt aagtcttatc aggttgaaat
     3421 atctgaatat cagcccaaag tgagcggata aacgggagaa gtgctttatc aaaaagtatc
     3481 tgattttctt cagatatatt cagagataag ataagttttt gactgagctc aatcaccaaa
     3541 gggttatctg ctacatggtt ttgataaaca acgttcaagt ctttttgtaa caattcaaag
     3601 ttatcgcata gataatttct agagttgaat agaaagtaga tataaaggca ttcatcttcg
     3661 tcatgacata aagagaaaga aagttgctct aatcctttct gtattagctg aaacaaggga
     3721 gtatgtcggg caactctttt ttcttcctga ctaaaatata ttgggttagt cttagatctc
     3781 tgcaaaccga tagcgatacc taataaaata aattgttttt caactgtagg gtaacttctt
     3841 gaaaaaaaat gtttttctac ataatcaatc aattgttgac cgctttgaat aattgattta
     3901 tttagctgga tcaactctgt ccgatttgtc tcatagatta tcgttagtaa caaacttcga
     3961 taatcctttt caggaatttc aaaatggcca tcagcattta agtactgcat ttgtttaaaa
     4021 aaattaatca actcatttcg taagttgtaa actttgctta acgaaatgaa ggtagtatca
     4081 gctatattac tccaattgtg ttcccctgat aaataatgtc ctagtacatt gaaaaagtca
     4141 gagcgctgat agatgagttg agtgagtgcc aacctcgaaa acgaagagtc gaatgcgact
     4201 gtaaagctac ttctagattt ctccactgaa ataatttgct gatggagtat gtcggtaatg
     4261 cattccaagt cgttagtgac agacagtgag tgggcaaacg ttcaattgat ctgctatttc
     4321 gcgaatctga atggtccctt tttcgtgtaa ttgctcacaa atgtaaactt gacgaaaaat
     4381 acttttctcc agataggttt ccaacatagt actcctccta gtcattttat acataactat
     4441 tataactcta ccaatcggaa tagtcctgta atatattgaa tcaaaaatat aaattataaa
     4501 ataattagat ggggtatttg gtttaagtat cccttatata gtaataaatt tataggtgtg
     4561 attctcgaat taatacccga aaatgtgaga gaatttttat ggatttttta ggatacaaat
     4621 taattatttt tgcttttatc ctgattacca gtccagtact gtcggtatcc cattttgcag
     4681 caaaatcatg ttgataaagt gacaagaaga ctgatgagat atattatcca aattgagaag
     4741 ataattaatc ggattcttga gtatacccta ctttttaaga aagtagggta tacaagcgta
     4801 tgaataaaga cataactttc cctgtgatgg acgttcggct tgttccaatt accaaaatac
     4861 aggcgaacga ttataatccc aatcgagtag cgtctccgga aatgaactta ttggagcttt
     4921 ctatcattga agatggcttt actcaaccat tagtttgtta ttacaaggaa aaagaagaga
     4981 agtatatctt ggttgatgtg cttcataggt atcgtgttgc taaggaaaga ctcaacttaa
     5041 aagttgtacc ggtaacagtt attgataaac ccatataaga acgtatggct tcaacaattc
     5101 gacacaatcg tgcaagaggg acccatgaaa taagaggaat ggaaattgtc atccaaagat
     5161 tagtaaaaaa tggatggtca gacaagagaa tatccaaaga attaggcatg gacattgaag
     5221 aaatttttcg ctttaagcaa gtgtctg
//
LOCUS       DANKAZ010000134         5189 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000134 DANKAZ010000000
VERSION     DANKAZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(406..780)
                     /locus_tag="PWY67_002576"
     CDS             complement(406..780)
                     /locus_tag="PWY67_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2058786.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="PWY67_002577"
     CDS             complement(803..2203)
                     /locus_tag="PWY67_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HDL2058787.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="PWY67_002578"
     CDS             complement(2207..2617)
                     /locus_tag="PWY67_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2058788.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="PWY67_002579"
     CDS             complement(2635..3126)
                     /locus_tag="PWY67_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2058789.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="PWY67_002580"
     CDS             complement(3136..3927)
                     /locus_tag="PWY67_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2058790.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="PWY67_002581"
     CDS             complement(3920..4693)
                     /locus_tag="PWY67_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2058791.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>5189)
                     /locus_tag="PWY67_002582"
     CDS             complement(4849..>5189)
                     /locus_tag="PWY67_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2058792.1"
                     /translation="TIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINL
                     FLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYEL
                     IYTIFEQTIF"
BASE COUNT         1657 a         1047 c          683 g         1802 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt
     5101 ttcatacaaa tatagtactt tttctatctc attgaatact tttttggaat ctaaaaagcg
     5161 aagcttagta ctagcgcctt caatagtaa
//
LOCUS       DANKAZ010000135         5175 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000135 DANKAZ010000000
VERSION     DANKAZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            68..886
                     /locus_tag="PWY67_002583"
     CDS             68..886
                     /locus_tag="PWY67_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058793.1"
                     /translation="MKEEDKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFN
                     SRNYTNENMELLRKDFEVVYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQF
                     VRTLWADGQVFLPERIYLLSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKS
                     LSLINSEQAGRQEIEIFVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDE
                     CLCAKKRVVLCDAASYQNGLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1107..2870
                     /locus_tag="PWY67_002584"
     CDS             1107..2870
                     /locus_tag="PWY67_002584"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL2058794.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            2954..3412
                     /locus_tag="PWY67_002585"
     CDS             2954..3412
                     /locus_tag="PWY67_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL2058795.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3521..4204
                     /locus_tag="PWY67_002586"
     CDS             3521..4204
                     /locus_tag="PWY67_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2058796.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4351..4965
                     /locus_tag="PWY67_002587"
     CDS             4351..4965
                     /locus_tag="PWY67_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL2058797.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            4981..>5175
                     /locus_tag="PWY67_002588"
     CDS             4981..>5175
                     /locus_tag="PWY67_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058798.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
BASE COUNT         1836 a          823 c         1069 g         1447 t
ORIGIN      
        1 agatgaaaag tcctttatca tacgaggaat agagatcgca atcggaagaa aggcatttcc
       61 ccttggactg aaagaagaag ataagaaaag cgcagaaacg aagccgttat ttcaattagt
      121 ctgtcaagga ttggaagagc aaaaaggaat tctagactta caagaagaag atgtctatta
      181 tattttttct ttgttcaaca gtagaaatta taccaatgag aacatggagc tgttaagaaa
      241 agatttcgaa gttgtttata aaaattttgt tctcaatgac ctatcactga atgaattgat
      301 tttacagatt attagtatcg ctgaaatcga cgatgcagaa aattttattt tcaagcaatc
      361 ctttttacaa tttgtccgta cgttgtgggc agatggacaa gtatttttac cggaaaggat
      421 ctatcttcta tcagaaaaag aacaacttct gtacggaaaa attgtagaaa tattggaaga
      481 gtggaaaaag aaaaatcaaa tccaactaag atggaatgaa aatttcattc gaaaattcgt
      541 caaaagtctt tctttgatca acagtgaaca agcggggaga caggaaatag aaatttttgt
      601 tgtttcagaa agttcagtca aacaattttt ttatcgaacc caatttcatt tgtatgcgga
      661 agaaggcatc gctgaaataa atcctatttt atttcgttca ttagaagaat tgcctgatga
      721 atgtctatgt gcgaaaaaaa gagtggtttt gtgcgatgct gctagctatc aaaatggatt
      781 agaaactgaa aagacaaaga tctatccaat ctcattaaaa ggtgcaaaca tccatttgta
      841 tcatattgct caggaacttc acctattaaa aaaacatatt tcttgatgag accttaatta
      901 ggagaccttc tacaaagctt tttaattgtc tgtagaaggt ttttatttga gtcctgattc
      961 gtgcgaagat ctacttatat aacaagcttt ctctgaaatt tttttaaaat gctattgact
     1021 tagagtcaac tctaagtagt agaatagata aagtgtaaca aatctatttt cattaaaaga
     1081 agatagtacg gaggagagaa tgtattatga gtacagtatt acatgtattg gatgcaatca
     1141 atagccccgc agatgtcaaa aaattatctg ttgaggaaat gaaacagttg gcagaagaga
     1201 tgcgccaaaa aatcttgcaa aaggacagcg atgtaggtgg gcacgttggt ccgaatctag
     1261 gtgtagtaga attaacgatt gcgtttcatt atgtatttga ttcaccaaag gataaaatcg
     1321 tttgggatgt ttcccatcag tcttatggtc ataagctttt aactggtcga aaaaatggtt
     1381 ttgaaaaagg acattaccgt gaagtgagcg gatattccaa ccaacatgaa agtaaacatg
     1441 actattttac agtggggcac acatctacat ctatcagctt agcaacaggt ttagctaaag
     1501 cgagagatct gaagaaagaa tcaggaaatg tggtagcatt tatcggtgac ggctccctaa
     1561 gtggtggctt agcttttgaa ggattgaaca atggtggtgt tttacaatcc aatctaatcg
     1621 tattagtaaa cgataatgag atgtctattg atgagaatca tggcagttta tataaaaact
     1681 tagctgagct tcgtcaaacc aacggtaaaa gcagcaataa cctatttaaa gcaatgggat
     1741 ttgattatcg ttacgtggat aatggaaacg atctggaaac ggtgatccgt ttatttgaag
     1801 aagtaaaaga tattgatcat ccgattgtac ttcacgtcca tacggaaaaa ggtcgcggtt
     1861 atcagccggc aatcgataat aaaatgaaat atcattggca tgtaccattt gatctgtcca
     1921 ccggggaggc aaagcaagta acttctggag aaacatttaa tatgatcatc atggattatt
     1981 tggataagaa aatgcaaaca agtacagctc ctattgccgc agtcaatgca gcgataccag
     2041 gcgcctttgg actgaaaaca ttccaagaaa aacatccaga tcattatttt gacgtgggga
     2101 tcgctgaaca gcactctatc actttcgctt ctgctttggc ttctcaggga gtacgtccag
     2161 tcgtcttcca atcaagtact ttcttgcaac gggcatatga tcaattatca catgatttag
     2221 cgatcaacga gaatccagtg attattttag tcggtggcgg aacgatctca gctagtgacc
     2281 gaacacacca aggaacattt gacattcctt acttatcaag tatcccgaac atcaaatatc
     2341 ttgctccagc aacaaaagag gatatgatct ctatgctaga ttgggcgttc gaacaaacag
     2401 cgcagcctgt agcaatccga attcctacac atggtgtaga acatggtaca gctatcgcaa
     2461 atgattatag tattccagcc tatcaaacag taaataatgg aggaaaagta gcaattttag
     2521 ctttaggcgg ttttcttgaa ttaggaaaag aaacagtaaa agcttttaga aaaaaaggaa
     2581 tcgaagcaac gttgatcaat ccaggaacga tcacagaaaa tgacgtagaa acattagata
     2641 aattaaaaga aacacacgaa ttagtcatca cattagaaga tggtagtttg tcaggaggat
     2701 ttggcgaaaa tatcagtcgt tattatggtg attcttcaat gaacgtatta aattttggtg
     2761 cagagaaaga attcacggat agcgttcctg tagggcagct ttatgaacgt tatcatttaa
     2821 caccaaaaca aatcgttgaa gatagtctgc gtgtcttgaa tattcaataa ttcattatgc
     2881 tcaccatcca gataattgtt tatactagta tctggatggt tttttcatta taaaaagcaa
     2941 aggggtggat gttatgtatc agcaagtaga agaatatgtc aaaaagttcc aagaaaaata
     3001 tcctagttat caatcagaaa aattaggttt cttttcattt ggggatacag aaaaaatggc
     3061 agacgagttg agcgaactag ttgtaaaaaa gataaagact ggtacatcaa gtggttacga
     3121 tctgtataaa caagagaatg agcctttacc aaaagttggt caattagacg tggtcctgaa
     3181 tggaaaaaat gaaccagttt gtatcatcaa aaatataaat gtgaccatct tgccttttag
     3241 tgaagtcacg aaagagcatg cttggaaaga aggagaaggg gatcggacat tagcttattg
     3301 gcggaaagtc catgctgagt tttttaaaca tgcttataaa gaagcgttga acatagaatt
     3361 tacagaagag aagctggtcg tatacgaaga atttgaagta attttctttt agtaagaaag
     3421 gtctgctgaa agaaatagct cattattgtc tcctgaatag aaaagggtta catttttata
     3481 tgaagctgta tttttatata acattactgg aggagatatt gtggatacac tggaacaaat
     3541 caaaggcgga gtcgtcgttt cttgtcaagc tttggaaaac gagccgctac acagttcttt
     3601 tattatgggg agaatggctt tggctgcaaa agaaggcgga gcagttggta ttcgagcaaa
     3661 tacgacagaa gatatcaatg aaatcaagcg aatcgttgat ttaccggtaa tagggattat
     3721 caaaagagat tacgatgggt cagaagtgtt tattactgcg acagaaaaag aagtggaaga
     3781 acttttggag acgcaagcag aaatcattgc actggatgca acttctcgta aacgtccgac
     3841 agaaaaaaca actgctgaac ttgtccaaat gattcacgaa cacggtcggt tagctatggc
     3901 agatatttcg acttttgagg aagcgcttca agcgcaagaa gatggttttg atcttttgtc
     3961 tacgacttta gcaggctata cagaatattc acgaaagacg cagacacctg attttgattt
     4021 attgaatcaa atcatagaac aagtcgatat cccagtaatt atggaaggac atactgactc
     4081 tcctgaacaa gtagagaaag catatgaatt aggtgctttt tcagtcgtag ttggatctat
     4141 tatcacccgt ccgcaattga tcactaaaag atatgtcgaa gccgcaagta aagcaaataa
     4201 ctaaaaaaca cgcacggcac agattcttga caaaaaagaa aaattgatag atagtttaaa
     4261 gcagattcta agaagatgaa aaaagcatta cttttcattt tgtcttcaag atctgctttt
     4321 taacgaattt tatagaaaga aggcgaaacc atgtttgaac ggagtccttc agaattgaca
     4381 gaacgtgaaa attacaagtt gctgattggt tcagtcatcc ctagaccagt agcagtagta
     4441 acgactaaat cagcaaaaga tatagtgaat atcgcaccct tcagttactt caatatcgtc
     4501 agttcgaatc gacccatctt atccttggcg atccagagaa aacaagatgc agtaaaagac
     4561 acagcaaaga atattcttga gacaagagaa gctgttatcc atatccttga tgaggacaac
     4621 gtagaagaag taaataaaac agcagccaat ctgcctaatg aggaaagcga actgtacctg
     4681 actgacttga ctttaactga ttcagtaatc atttcagtac cagcattgac agaagcaaaa
     4741 gttcgttttg aaacatcctt atatgaacat gtcgagatac ggaataaaaa taaagtaaca
     4801 gctgatttat ttttgctaga agtacagaaa tatcatttaa atgaaacagt ctatgatgat
     4861 aagacaggaa ggatcgatcc aagaaaattg aatgctgtaa gccgtttggc aggaaatgat
     4921 tatgcagcaa taggagaaat tttcacgatt gcacgtcccg aataaaaagg agcactagtt
     4981 atgttaaaaa taagctataa accatcgaca gattcaaaag aaatgaaaaa agaatatgaa
     5041 acagtgaatg attttctgca agggcagtac ttagaagttc cgccattgca agaccatttt
     5101 gtcgttacta ctgttacttt agatggtaag gaaatagaaa tgccagatca aacgattagc
     5161 ggattgttta attac
//
LOCUS       DANKAZ010000136         5118 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000136 DANKAZ010000000
VERSION     DANKAZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            229..573
                     /locus_tag="PWY67_002589"
     CDS             229..573
                     /locus_tag="PWY67_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL2058799.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            574..993
                     /locus_tag="PWY67_002590"
     CDS             574..993
                     /locus_tag="PWY67_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL2058800.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1085..1435)
                     /locus_tag="PWY67_002591"
     CDS             complement(1085..1435)
                     /locus_tag="PWY67_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058801.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1401..2621)
                     /locus_tag="PWY67_002592"
     CDS             complement(1401..2621)
                     /locus_tag="PWY67_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL2058802.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2987..3553
                     /locus_tag="PWY67_002593"
     CDS             2987..3553
                     /locus_tag="PWY67_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2058803.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3698..3785
                     /locus_tag="PWY67_002594"
     tRNA            3698..3785
                     /locus_tag="PWY67_002594"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3730..3732,aa:Ser,seq:cga)
     gene            4119..4685
                     /locus_tag="PWY67_002595"
                     /pseudo
     CDS             4119..4685
                     /locus_tag="PWY67_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            4798..>5118
                     /locus_tag="PWY67_002596"
     CDS             4798..>5118
                     /locus_tag="PWY67_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2058804.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT         1869 a          861 c          969 g         1419 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgtaaaata
       61 aaaaaagcgt tagcaactat gcttaaaaag catatcgact gaataaacta agcattggac
      121 aggctgaaag actacattta aaatgaaatg cgaagaaaca atagaaaata aagttgcctt
      181 agaaccatag ttgaatcacc taacagtcgt gaagggagaa taagagaaat ggcagaaaaa
      241 caaaccgcag gacgagatca attaggagat tttgcaccaa aatttgcaga attgaatgat
      301 gatgttttat ttggacaagt atggtccaga gaagaattat ctgctcatca gcgtagtatc
      361 atcaccatat ctgcattaat ttcaggagga aattttgaac aattgccagc tcatctgaag
      421 attggcaagc aaaatggcat tactagtgaa gaaatagcag aaatcattac ccatttagct
      481 ttttatgtag gatggccaaa agcttggtca gcttttagtt tagcaaaaga aatctattta
      541 gaagaaaata gcgaagataa gaaagaggcg taaatgacga caaaacacga agaaatacac
      601 aatggcgtga tttttccagc aggtgaaaaa aacgaagctt acgctcaagt atttaccggg
      661 caaagctatc tgaatatgtt agtgaatgat cccgaagttt ctgttggtgt aggtaatgta
      721 atttttgaac caggttgtcg caatcactgg catgtccata aagacggcta tcaaatctta
      781 cttgtgaccg gaggagaagg ctggtatcag gaagaaggaa agccagcaag acgattaatg
      841 gctggagatg tcgtcgtaac gcacaaaggg attaaacatt ggcatggtgc gacaaaagac
      901 agttggtttg aacatttggc gatcactgct ggtaattcag agtttttaga acctgtaaca
      961 gatgaagtgt atgaaggatt ggaaaatcaa taatgtcaag cttgaacagc tcctgaaagt
     1021 agattttaat cgaactttca ggagctgctt ttttgattca gtcttttata aatagaaaat
     1081 agggttaata ataggaatga gaagcacgtc gaaagatctt aatattgata atcggctggg
     1141 catacatgat ttccacgtca tcaatcaaac tacctaacag atgataatct tcccgatcat
     1201 gtcggggatt acctaatagt tcttgatagt agatttccat actagggtcc cgaggattgt
     1261 tcagcaaatc agcaagtaca tctaaatctt ctggctgagt ttctgccgta ccctgtatct
     1321 ctatgaaaag cccttcctca gcaaattcta ctccaatacg tatttcagtt atttcatggg
     1381 aatataaata agtaagaagc tcatcgatga ttctttttga taattctttt tccaagtgtc
     1441 tttttttcct ttctgacagc ggtaaagtta tctagtatcg gtacaaggat acctgcaacg
     1501 aaaccagttg aaaaaccact gttatataga tttagtcctc cgtgcaaagt gctgacgtta
     1561 tggacaagag caatatgtag tacaccagct attacaccgt aaaaactgcc gtaaaaaccg
     1621 cttatgggcg cgagacctgt cccaaacaca gctgcaacaa gcatgccagt actggtgatc
     1681 tcatgaaaag ttccaaacaa agaagcaata aatattccga ctagtatcgg aaaactgttt
     1741 tttaaatgac aaccaaaagc actgaaaccc accgcagata aaatagcacc gatgacaggt
     1801 ccatttaact gaccgccaac cagcaaaaca taagaagaca gcatcagacc aactaagcct
     1861 atgttgatca aagtagcacc aataccagaa ttagcaatga aatcagttgt caattttcca
     1921 gatgaatcga aaatttctcg aatccccaaa agccgaaagg aattataata gaaaccaatt
     1981 ccgactgtca gcgaaaaaaa gccaaaaata atccaaatga gaggaaagtg atattcattg
     2041 aaaaccaatg tattttcgac gattgaatag ctgaataacc tcaaaacagc agtaaaagtc
     2101 atcgcaatca acccagaggt gaatccaata ttataaagtg tgaatccacg atggaaaact
     2161 aaagtttgag cagctaaggg cggaagaagt agaccaattg ctatgccagc taaattcccc
     2221 aataaaatac ctatcagtaa aggaaaacgc ataccaaaag tgatataact aacgaccggt
     2281 gataatgcgc taccaaacag tccgatcatg atgtactgag aaaaaggttt attgacaaat
     2341 acagcgtata gatacacacc taaaatgatt gagatagaat tataaaggtt tttgccaaaa
     2401 aaagagaaac cggtaactgt tagaatcgaa gcaaataagg gaccgtttaa aatcaccttg
     2461 tgccgataag ctaaaagcac agaaagtaag gtcaaaatcc ctacatttat gaatgcgctt
     2521 ccgaaaccgc ctaaagcaaa ataatctgtc aaaagattgc ttggcgaggt aagaatagtt
     2581 agcaatccac tccaattatc agtaggagta ttagaaagca agccaataaa aagaaaaaaa
     2641 cacctgtaaa accaagataa aaatactttc ttttctgtga aacgattcgt ttttgaggat
     2701 gaatataaat aacgtcagtt tcgttttcca ttacacacct tctttttcat tttctttaac
     2761 tataacttga aatgaaagat ttggacaact atcatttagg tcaaaactaa aaatgtaagg
     2821 gaacccattg aatttaacca aattttttta attttgcaaa aaaaagttaa agaagactga
     2881 aaaaaaattt aaaatataac gttttgagta aaatgtttgc aaaaaatgct aaaaataggg
     2941 tacaataaaa caacatatac tgcagtaaag aagtggggga gttttaatga gaagaaaagt
     3001 ctataccaag gatcaaattc taaaagctgc ttatgatgtt gttgcaaagg aagggtttaa
     3061 aggatttact gcgagaaata ttgctaaaaa gatggggatt tcgacacagc cgatttatct
     3121 ggaattcaaa aacatggaag atttaaaaaa tacgttgctg gattctatct ttgaacaact
     3181 aaaagaagat gttttcccta ttgaacgtac tggtgataaa atcattgatc tcggtttgaa
     3241 ctatatctac tttgcaaaaa agcatcatag tctttacatg gcattgtaca ttgatgaaca
     3301 tggggacgga aagaaaatgc atgatttttc atacaattat ttccgtgaaa tagttaaaga
     3361 agatgaaaga tacaatcaat tatcggatga atatgtcgat gctctgcata ctggtacatg
     3421 ggtagcagtg actggcttag cgtcattgat gtcatcaaac atcatgcatc caacagaaga
     3481 acaaatcatt gcactgatca aacggacaat tgactcgatt ttagcattag ataatcctga
     3541 aataatcaga tagtgttcta tcagtatgaa tttgatcggt aagtccgttg gctgcaaact
     3601 gtcaatggac ttttttgcta aaaaaagcga tttctgttat ttatctatta gaaataggct
     3661 tgacatttcg tgaaaaaagt ttaaaaatag tacttacgga aggttggcag agtggtaatg
     3721 caccggactc gaaatccggc gaaccggcta ataccggcgc gcaggttcaa atcctgtacc
     3781 ttccttatat caacgattag aggttgtgaa gaagctgtcc tgcatcaagt aataagaacg
     3841 gccaaacaaa aatagctttt cctatttttt cgtttgattg ggcttattac cgcagatgca
     3901 agcttttgaa caccgtttgt tcggaataag gggctgtgac aagacttttg tcacagcccc
     3961 ttattgttgt acttttaacc tacaatattt tttgattccc cctgagtact attttttata
     4021 aatgctagta aatcaatctt ctttggcctt tttttgataa aaaaaatact agatgtaaga
     4081 taaaagtgta aaaacgattt gttttaggag gggaaacgat gtgtaccgca atcacttatg
     4141 caacgaaaga ccattatttt ggaaggaatt ttgattatga gatgtcttat aatgaagtgg
     4201 tcactattac tccaagaaat tatcgtttcg attttagaaa ggtaaaaaat ttagataaac
     4261 attatgcaat gataggaata gcagctgggg tatccaacta tccactctat tatgaggcta
     4321 caaatgaaaa agggttgagt atggcaggac tgaattttcc tggcaatgct gattataaag
     4381 aattacaaga aggaaaagat aacgttgcac catttgagtt tattccatgg atactagggc
     4441 aatgctcaac catagacgaa gctaaagaat tattggctac tatcaatcta gtcaatatcg
     4501 attttagtga aaaactaccc ttgtcacctt tacactggtt attagctgat aaagaaaaat
     4561 cgatagtaat tgaaagtatg aaagatggac ttcatttata tgataatcct gtcggtgtgt
     4621 tgacaaataa tccgccattt gattatcaat tatttaatct gaacaattat cgctcgctat
     4681 caaatggaac gagagtataa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
     4741 gtcccttctg tagaataaag ttaacgacaa ccaattcaca gaaaagagga cttccctatg
     4801 aatgatttta ctacagaaat tgtgcaaact ctagtcacta aaggcgattt aaatgaatta
     4861 ttccgttcgc acttagaaaa agcgataaac acactcctac ggactgaatt aacggctttt
     4921 ttagattacg aaaaatatga tcgcactggt tttaattcag gtaattcgag aaacggttct
     4981 tactttcgat caatcaaaac cgaatatggt gaattaacat tggaaatacc tagagatcgt
     5041 aatggtgagt ttaaacaaca aactttacca gcctacaaaa gaacaaacga tacattggaa
     5101 accactatta tccattta
//
LOCUS       DANKAZ010000137         5109 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000137 DANKAZ010000000
VERSION     DANKAZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..152
                     /locus_tag="PWY67_002597"
     CDS             <1..152
                     /locus_tag="PWY67_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2058805.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="PWY67_002598"
     CDS             complement(230..763)
                     /locus_tag="PWY67_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL2058806.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="PWY67_002599"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="PWY67_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL2058807.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="PWY67_002600"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="PWY67_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL2058808.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="PWY67_002601"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="PWY67_002601"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2058809.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="PWY67_002602"
     CDS             complement(4488..5066)
                     /locus_tag="PWY67_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL2058810.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         1469 a         1049 c          918 g         1673 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgcttta
//
LOCUS       DANKAZ010000138         5018 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000138 DANKAZ010000000
VERSION     DANKAZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            551..1462
                     /locus_tag="PWY67_002603"
     CDS             551..1462
                     /locus_tag="PWY67_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HDL2058811.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1949..2215
                     /locus_tag="PWY67_002604"
     CDS             1949..2215
                     /locus_tag="PWY67_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058812.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2287..2628
                     /locus_tag="PWY67_002605"
     CDS             2287..2628
                     /locus_tag="PWY67_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058813.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2683..3102
                     /gene="ssb"
                     /locus_tag="PWY67_002606"
     CDS             2683..3102
                     /gene="ssb"
                     /locus_tag="PWY67_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2058814.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3179..3475
                     /locus_tag="PWY67_002607"
     CDS             3179..3475
                     /locus_tag="PWY67_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058815.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3490..3921
                     /locus_tag="PWY67_002608"
     CDS             3490..3921
                     /locus_tag="PWY67_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL2058816.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4103..4408
                     /locus_tag="PWY67_002609"
     CDS             4103..4408
                     /locus_tag="PWY67_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058817.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            4812..>5018
                     /locus_tag="PWY67_002610"
     CDS             4812..>5018
                     /locus_tag="PWY67_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL2058818.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINP"
BASE COUNT         1860 a          752 c         1043 g         1363 t
ORIGIN      
        1 gccacttatg agagaaaatg gtcaaaaacc taagcgggaa gtctccgaat aacataccaa
       61 gcgccgacag ccaacagata aagaatacac tactaaaaat agtagtagca taaaatttaa
      121 aagggaaaaa atcaacccct accccagtct tggggtctca aaactaactc ccccctaacc
      181 ccctctttca gcctcttttg aaagaggggg agaatcaacc ccacggccaa cgccccagtg
      241 ttcttggggc tgtccagaac agaaacttgc gagagagtgg gggtaaaaca agtgtagagg
      301 aaacttgaag acttgttcga acagaaactt gcgagagagc cggagtaaaa caagtgtaga
      361 ggaaacttga agacttgttc gaacagaaac ttgcgagaga gttggagtaa aacaagtgta
      421 gaggaaactt gaagacttgt tcgaacacaa acttacaaga gaggggagta aagcagttgc
      481 agaggaatca ggaagatttg ttcgaacaga aacttgcaag aaagcattat taaattgaag
      541 gtgatgaaat atgaagtatt tattgcatgg ctattttatt gattattcta ctcgcaatag
      601 tagtacttta gcgattctat gtttgcttca tgaattgaga gtggttacta gaaaacagct
      661 atacaaacta ttgaattttg acagatcaat tagtaaacaa ggagtaacga atgctttagt
      721 gagattatat gaaaaacagt taatcgatcg aaaaaaatat ggaacagaaa tgtattatta
      781 tttgacgaaa gagggacatc aaagcatagg tgggtattat acgttgccga aagttcctga
      841 atataatttg aatcaccatt tacaaattaa caattatttg attaaaggaa tggcattggc
      901 aaaagaccac ccacatttaa aacggattat ttcagaaaga cgtaccgtct atgaaacgaa
      961 ggatttaaat cctaagacaa aaggaagaaa attttttgtg ccagatttta tttttcgttt
     1021 tcgagacaaa gaaaatcatg agatcaattg gtcctttgaa attgagctaa cgttgaaaac
     1081 acgtcgtcgc taccgtgaag cgatttttcc gaaatatatt cgtgagttgc gaacacgtcc
     1141 tgaatcacat ttaatttatg tgacacctag tggcgcaatt caagaagaac ttcatgcgtt
     1201 taagcgtatt tttttcaagc gcgaaactga aaaagatgcg agtcaacaag aattgtttga
     1261 gcggttgcat attttttcag ctttagagtt tgaagaggga ttaaaagcat tattagagaa
     1321 tgatccagca gtcaattggc ggacagtaga acgagaaaaa atttatccga atggcgctgt
     1381 tcaaagtcgt ttgaatttat ttgaagaatc aactgaaaat gaggaaaaac caaaagtgaa
     1441 gtttacgcct gaagtattgt aaaagagggt gttcgcttcg ctcttttttt gtgccagttt
     1501 gctggcaccg ttcagacgca agcgtctgaa ccggctcgtt tttaggaagg actaacttgt
     1561 ttgatttatc cgttgtcaaa gaagtggacg ttcgagagtc cctaatcgta tagcagtcat
     1621 tcggcgagta taacctaaaa cataacctgc caattgccaa gggtaaaatg cacgctgcgc
     1681 gcccttggca atcgtcagaa catgttttcg agccggttat gccgaacgac cgctatacga
     1741 aaagggcctc taaacagaag tccactttga tccttgacaa ctgaaaccca aaatcaaaca
     1801 agcaggcctt cgtctaaaaa cgagccgaat gaagaccttg ggggttgcac ccccaaaccc
     1861 ccgtgccccc gacacactac cgaaaaggtg ggggtgggtg gcgaaaaaat caaataaaac
     1921 attctattca agaaaggaag aaacaaccat gacaactaaa aacgagcaac tgaaagaaca
     1981 ggcaagaaaa gagttgcaag aaagaggttt gattattgag gggagctttg aaggagattt
     2041 tgaaacctat attggttgtt atgcaagacc gataaacaaa cctacagcct tagacccaat
     2101 gaacgaacaa gaagtaaaag aacaagaaaa acatgcagtg aatggttttc cgcaagattt
     2161 cactgaatgg tatgagtggg aaattaacaa cgggaagctt gaaaacttct tataaaagtc
     2221 agatgacgtg caaagagaat gattctcttt gtacggtaaa aatataaaga aagaaggaaa
     2281 aagaaaatgg caaaaaacac accttatcaa gtaggagaca ttttttatag tagttggggc
     2341 tacgaacaaa cgaacgtgac tttttggcaa attgtgaagt taacagaaaa aacagcatgg
     2401 tttcagccta ttcaaaagaa aacggtcgag cattattcgt ccatgagtgg aaaagttgtt
     2461 ccaatgaaag acaagtttat agagtatcct ttagcaaaaa ctaaagacag tattgtaaaa
     2521 aaacgaattg atccagaaac ctatcctgat atgttatgtg gttatcgtag ttgggacacg
     2581 tttcatagat ataaaaatca agctgtttat gaaagtagtt attattaagg ggatagtttc
     2641 ccctttcaaa aataataaat agaagaatgg agagaaaaaa tcatgatgaa ttcagtgaat
     2701 ttagtaggac ggttaacaaa agaagtggat ttaaaataca caccaaaagg caatgcgaca
     2761 ggcacattta ttttagctgt taatcgaaat tatacgaatg caaacggcga gcgtgaagca
     2821 gattatatcc gctgtgtcat ttggcggaaa gctgcggaaa ctttagcaaa atatactgga
     2881 aaaggaagtt tgattggcat taatggacgt cttcaaacga ggagctatca gaatcagcaa
     2941 ggacagacca tgtatgttac ggaagttcta gtgacagatt tctatttgtt agagagtaaa
     3001 gaaatcaatg agcaacggtc aaaatcaatg gcgaataatc aaaataattc ttcaattcca
     3061 ggaaatgaga ttcaaatttc agatagttat ttgccctttt aagtgaaaaa taattttgtt
     3121 ctatacctga tttcttcagg tatagaacaa aaaataaata gagatgaggc gattttttat
     3181 gaaaatcatt gacagttacg atcaacgaac catgcaatat ttgggctcgt ttgattatac
     3241 agatgaaaat attattaatt atgttgcaag tttacctcct tatcaaagcg ttcgcttggt
     3301 agattttgca agtgatgaat tggtattaac aacgatgggc aactttttag attatgtcgg
     3361 agacaaaaaa tggttggaaa aaattttgtc taaattgatt gcgaaacaaa caggagaaga
     3421 acccataaat gcgattcaat tgttcaatcg atatgaacaa gaaaacaatg attaaagagg
     3481 agagaaaaaa tgaaaagatt aaaacgaagt atttttattg tgctagcatt gattgatttt
     3541 gttgggacag gttgtgaagc aattgacgtt caaaaagctc ccttatcgga aataaaacaa
     3601 gcaataacaa aacaagatga agaacaaccg ctaaatacac aagtagaaat agtgaatcaa
     3661 aataaaccga gtttttctga agaagatttt tcgttagaaa atggaaccag gcaatttttt
     3721 tcagatttgg atcgtttaaa tcgggtaggt gtagcaagtg cacttttgca taaaagcagg
     3781 atgccaacag aagaacgaga ccgattatac attaagccaa ctggctggaa acaaaagaaa
     3841 atgaagaatg gcgattatct ttataatcgt tgtcatttga ttggctatca acgtgcgaca
     3901 agtttcccca taagtatgta atacgaaacg ggaggattaa tcttaagatt aatcacaact
     3961 aacctatttg acagttggaa tgaaagctat catgtctgct gaatcaactg gtctgatact
     4021 cctacatgca ataaagtcta ctaggaagtt attgtatgaa gctaggtaaa ggcggcagaa
     4081 caatttctaa gtgtggaatc atatgaagtt ggatatgccg ggacgctata aattactcag
     4141 ggtgagactg tttttaatta aactgacgaa cttgcgaatg tacgggtcta taaccgactt
     4201 atattcgaaa gatataagat tgcgaatgca aagaacatta tcgaaaagta agaatgttgg
     4261 tcatgaaatt cgagatggaa gactagcggt gtatctgtca agagttggta aaacatgttt
     4321 gattgattca gatgagagtg ggaatgcgac aaaacgtttt acagaagaaa tagagcaact
     4381 agcagaactg aatatacgat atttttaaat actttagcgg agaataaaaa aatcctctac
     4441 agaaaactac ggagggcaac atgtgctatt gtttcaacaa tacattttta atttttacca
     4501 taacaaaaaa gtacttgaaa aaaaacagaa atatgttaaa ttaaaaatga aaaaatggaa
     4561 gcggttactt attcttttga atctaagata aaaattcaaa agaataacat aacccaaata
     4621 tgattatact tgagattcta aatatttatc gaaaaggaat ttgttttaag gaaaaatgat
     4681 tctatggacg gttattgatt tatatttacc aaatggctaa taaaatagta tttcttatat
     4741 tgtatagttt ttgtttttat gtgaaaacat gagcaataga attgtactag taggataagg
     4801 atggatggaa aatgaaaaaa actatttttg ttttagcgat ttttatatgt tttgggctac
     4861 ttttggtagg ctacttgttg tttaattcta atgaaatgag aactatttct acgaatgact
     4921 tgttaaaaaa aagtgaatta aaggataaaa aaaaagaatc tcttgccttt ataatgttta
     4981 ctcaagaaga gtgtgagtat tgtatagcaa taaatcca
//
LOCUS       DANKAZ010000139         4947 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000139 DANKAZ010000000
VERSION     DANKAZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            217..480
                     /locus_tag="PWY67_002611"
     CDS             217..480
                     /locus_tag="PWY67_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058819.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            504..692
                     /locus_tag="PWY67_002612"
     CDS             504..692
                     /locus_tag="PWY67_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058820.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(775..960)
                     /locus_tag="PWY67_002613"
     CDS             complement(775..960)
                     /locus_tag="PWY67_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058821.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1175..1546
                     /locus_tag="PWY67_002614"
     CDS             1175..1546
                     /locus_tag="PWY67_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2058822.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1696..1768)
                     /locus_tag="PWY67_002615"
     tRNA            complement(1696..1768)
                     /locus_tag="PWY67_002615"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1733..1735),aa:Gln,seq:ctg)
     gene            complement(1814..2242)
                     /locus_tag="PWY67_002616"
     CDS             complement(1814..2242)
                     /locus_tag="PWY67_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058823.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2302..3060)
                     /locus_tag="PWY67_002617"
     CDS             complement(2302..3060)
                     /locus_tag="PWY67_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2058824.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            3355..3552
                     /locus_tag="PWY67_002618"
     CDS             3355..3552
                     /locus_tag="PWY67_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058825.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3658..4140
                     /locus_tag="PWY67_002619"
     CDS             3658..4140
                     /locus_tag="PWY67_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058826.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(4198..4794)
                     /locus_tag="PWY67_002620"
     CDS             complement(4198..4794)
                     /locus_tag="PWY67_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2058827.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         1601 a          775 c          956 g         1615 t
ORIGIN      
        1 agaatgctta tcataataaa aaaaggaata taaaaaacaa attgaccgat cgcgatcata
       61 ctattgcctc cgataaataa agtccatgat acaaaataac actttgtgcg aaatggcgca
      121 agcagttttg tggatttatc cggtaaaatc tgtatcgatt ttgaaattag aggagaaact
      181 tgctataatc aaagtgaaag aaggagtggg attttcatgg atcatgtagt gttagaaatc
      241 gatttgcatt tgatcaataa tattcgggtg atttactatg ttgtaagtaa cagtgtcgaa
      301 caacgtgtac tgacgaataa aataaatggt attttagcaa agaaagacgt tcaccgattc
      361 aataatggtg aaggaagtta ttatagtatc ccagtagaaa aaattattta tacgacagtc
      421 aaagtcagag aagatctaga taccaaaaaa gcatatgaac ctatttttac aacttattga
      481 ttgacagcag atggaggaaa aaaatgaatc catatgaaca acttattcaa gatttgatcg
      541 atggaaaaat cgaaaagatt gatgtaaaaa gagaagaatt aatggctttt cgagaagcgt
      601 ggctgaagtt ggagaatcgt aaatatgtgg tgggaaaagc ctcactgaac ggcaatgtga
      661 cttatcatta cgatccgaca cgtttatttt agtatgaagt attcagaaaa gaaccaaagg
      721 aggacgatgc agcagcattg tcctcctttg attttacaaa agtgaaagtt ttacttattt
      781 gaatagattt ttaaatttgt ttttgacact gtcgatattt acatctttta aaagattttg
      841 ggctttctct ttataaggtc ctaattgttc tttatgttct tcgataaatc gttttgcgcc
      901 atcgaaattg ccgttcttaa tcatttcctt gactttttga aataattctt cttgattcat
      961 tttgaacaca tcctttgcta tcaacatgat tttttacatg gacaaaacga gaaaaactga
     1021 tttatccaca cgtcaataat atcaaataac gatcattttt actaaaaaaa cactcgattc
     1081 tcgcttctaa agtaggagaa aaatctccaa cttgggacgg atggaatttc tttaactgac
     1141 ccatatactg aatatataac aaggaggtag aaagatgaaa gtgattaaat atttgattgg
     1201 actggcggcg attagtggta ttttgctatt tggactgaaa tattatactg aaggaagtta
     1261 tggagaaatc cctggtatac ttgatcagct aaatccatta gttgaaaaag gagaggtcta
     1321 tgtaaagaca caaaaaccag aggaagtaaa tgaatatggt acagcaaggt atgtgcagaa
     1381 agctgctgat gccaatggga aagaacgaac ggttgaattt aatggtcttt ctgtgttaaa
     1441 agaaaatcat tatttgaaaa ttagcaataa aggagcacac atagaaacgt atgaggaagt
     1501 tcctagagaa aaagttccgg agaaagcatt agcaattatt gggtaaatca gtttttgtat
     1561 taagaatacg cccgatttta ttattcataa tttaattatg taaactaaat acaagtagta
     1621 aaaataatac aataatcccc ctgcaaaaag attgttcttt tacagggggt tcattttttc
     1681 agcaagcaca tcaaattggg atggcagggt tcgaacctac aattgcttga ttcagagtca
     1741 agtgccttac cagtttggct acatcccatt tattcaacaa aataatcata gcactttcct
     1801 aaaaaaacgc aagttatttt tcttgatcgg atagttgttg ctttactcgt aaaaggcggc
     1861 gtactagttt cttttctaat ccagcaggaa gggcaatcag ttctgctact gacaagacgg
     1921 tgtccaataa ctgatattct ttgacaggtt cattcataat atgatagatg tttgctaaac
     1981 gttcaatata ttcaaagcga gtatactctg ttcgagcaat tagaaatttc aataaatcgg
     2041 tggctttctc gtactcttga tctgcgtaat aaacaagaga ttgacgatac aatttgattt
     2101 ccagttcatt gagggtagca ggtctgcgac gctcgttcca ctcattaata gagactttac
     2161 cttgaacatt tcccttgcag tcacggacaa aaataatcgg aatttcattt ggatccattt
     2221 ttttatgtgt gaatttttcc attatctacg ctcccttcaa aggtttggta tcttgagtat
     2281 aagggatttt tgtttgaatt actagctttt ctccaaagtt agcatctgtc cacccattgc
     2341 agtgaaaatc gccgaaaaat cgttgatcgg tactgctcga ttttttacac catagggatc
     2401 gttgacataa atattttctt tgtcatatcc agtgactaca caagcatgaa caagaggagt
     2461 gactcttact tctccattat ttgtggtcca agtcataaaa tcagtttcat ctgggacttc
     2521 aaaattgata gtcacagaag tccagacagg atttccttta gatagtgctt ttttgatatc
     2581 atcgagttga gcatcggttt ttggaaaagt gtgataatcc gtttggacga tttgctgagc
     2641 aagggaggca actgggggaa catagacgac catagcccag tctccgccac tgatatcacc
     2701 aacaaatcct tcattaggat cgccatgagt cccatctgca ttgaaaacag ggacgtaatc
     2761 caattgctca gctaactggt ttttagttgt ttcataacca taaaaagata aaagcataga
     2821 aagcgaagtg acttcacagc cattttctaa agaaggagaa tcaaactggc tctctaatgg
     2881 aacatctagt aaaacttctg ctttctcttt tgtctttttc gttttttgtg ttgaactttt
     2941 ttgtgcagtg ttcattgtat gttctggaga atggatatgt aggtaataac ttgtaccgct
     3001 taataaaaga gcagctgtgc cccctagaaa caatagtttt ttgaacgttt ttttcttcat
     3061 ttgaaatctc ccttttcagc tgtttatctt tcctattata aatgtatttt tattggaaat
     3121 ggaagtatta tattttaata tgtaggaaat aattagtttt taaggaatat gttttaattt
     3181 tttgaaaaaa attcttctaa atacaacagc tgttttaatt gtgaaaacga aaacagtacg
     3241 ctataatgcc tgtaaggttg atttttaact gtaaataaaa atcaatgagt taaatctcca
     3301 aagaaatagc agtggtaatg tggagaaaag ttccagcagt ttaggaggat tttgatgacg
     3361 gaagaaatga ttaatttagg agaacaatat gcatgcaaac caattggttt tacaaaaacg
     3421 gtcattggtg aagttgtatc aaaaatgaca aactgcgccg tggtcaaagt tgcccaatgt
     3481 gctgcagagg accaagaatt attagatgaa aaagcaagca tggttgttgc aaaatatgat
     3541 acatttgaat aaagaaaaca attggacgtg cggcgtgctg caagtccaat ttctttgaga
     3601 aggtaaatca gtattctttt cctattttct ggagaagaaa aagcgaggcg agttgtcatg
     3661 gtaacattct ttacggttct attgttttta ttcgttgttg tgatgttttt atttctttgg
     3721 ctggttcgaa aagaaattat ttatcggact gtgcgaaatc gatgggtcta cttggtgata
     3781 cccttcttag tgtcactagt catttggtat acactcatca gtcatcctac agcagatgaa
     3841 ttggcaaaag ggatcttgtc agcaatgatt tttattagtt ttttgttgga cagtagagga
     3901 atcacagaag aaggacttgt tttgaatagc tttgacaaaa aaggtgttcc tttttctgag
     3961 atcaataaaa ttgtattata tcagccaaaa ggttctaaaa tcgtaaaaat gaattttttc
     4021 cgaaacggat ggcgaggacc gatgctgaaa ttttctgcat cattagaaga attagttcca
     4081 tttttgtccc aacgcttaaa cgatgaggca gagattgata ttatgattga tccagaataa
     4141 aaacagaggt tgcgacaaga tgtttgtcac aacctctgtt ttttatttca aataaaatta
     4201 tccaaatacg cggaagcgat cggcatcgtt catgtattct ttttccccag ccggtgtttc
     4261 tggtgaaccg aatactaatt gcgaacgtag tttccagttt gaagggatat tccattcttt
     4321 tgcaactgct tcgtcaataa ctgggttgta atgttgcaag tttgcgccca atcctttatc
     4381 aactaatgct acccaagtat tagctgttgc gattccgttt gattgttctg accaatctgg
     4441 gaagttgtct gcatataatt caaattgttc ttgtagacct tttaccacgt cagtatcttt
     4501 gaagaataat actgtaccat aaccattttt gaaaccagct aatttgtttt gtgtatttgg
     4561 gaaagcttct gctggagtca atgggcgaag tgcttcttca gtgatttccc aaagtttttc
     4621 atgtgcatct ccgaaaagga taactgcgcg tgttgattga gcattgaaag cagttgggct
     4681 ttctttaatt gcttctttga tcaaagttgt taattcttca ttagaaagtg aaacattacg
     4741 tcctagatga tagattgaac ggcggttttt caaagtgtca gtaaaagttg tcataataaa
     4801 atttctcctt tgatttgcgt tttgcatttt tttaattgat tacgaaatga agtatagcac
     4861 cttacaaaaa gtaagtaaac agatatgctc acaaaaaata agcgaagttc aaaaaacaat
     4921 tttaataaga aaaatcgtta tgtaagg
//
LOCUS       DANKAZ010000140         4710 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000140 DANKAZ010000000
VERSION     DANKAZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            106..357
                     /locus_tag="PWY67_002621"
                     /pseudo
     CDS             106..357
                     /locus_tag="PWY67_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="PWY67_002622"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="PWY67_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL2058828.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="PWY67_002623"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="PWY67_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="PWY67_002624"
     CDS             1786..1869
                     /locus_tag="PWY67_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDL2058829.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="PWY67_002625"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="PWY67_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2058830.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="PWY67_002626"
     CDS             complement(2994..3857)
                     /locus_tag="PWY67_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HDL2058831.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="PWY67_002627"
     CDS             complement(3859..4131)
                     /locus_tag="PWY67_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2058832.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="PWY67_002628"
     CDS             complement(4148..4357)
                     /locus_tag="PWY67_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2058833.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="PWY67_002629"
     CDS             complement(4455..>4710)
                     /locus_tag="PWY67_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HDL2058834.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DANKAZ010000141         4611 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000141 DANKAZ010000000
VERSION     DANKAZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(86..340)
                     /locus_tag="PWY67_002630"
     CDS             complement(86..340)
                     /locus_tag="PWY67_002630"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2058835.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="PWY67_002631"
     CDS             complement(451..705)
                     /locus_tag="PWY67_002631"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2058836.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /locus_tag="PWY67_002632"
     CDS             complement(898..1173)
                     /locus_tag="PWY67_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL2058837.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="PWY67_002633"
     CDS             complement(1145..2098)
                     /locus_tag="PWY67_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2058838.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="PWY67_002634"
     CDS             2710..4203
                     /locus_tag="PWY67_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2058839.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="PWY67_002635"
     CDS             4338..>4611
                     /locus_tag="PWY67_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2058840.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DANKAZ010000142         4593 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000142 DANKAZ010000000
VERSION     DANKAZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            72..1118
                     /locus_tag="PWY67_002636"
     CDS             72..1118
                     /locus_tag="PWY67_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2058841.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="PWY67_002637"
     CDS             1135..2358
                     /locus_tag="PWY67_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2058842.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="PWY67_002638"
     CDS             2447..3334
                     /locus_tag="PWY67_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL2058843.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="PWY67_002639"
     CDS             3420..3650
                     /locus_tag="PWY67_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL2058844.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..>4593)
                     /locus_tag="PWY67_002640"
     CDS             complement(3707..>4593)
                     /locus_tag="PWY67_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide excision repair endonuclease"
                     /protein_id="HDL2058845.1"
                     /translation="VGKAKRLKNRVSSYFHQNKQHSKKVLRMIHHITDFDFVVVDTEL
                     DALLLECQLIQHYRPFYNRQMNYFSNYNYVHITNKGFVLTDTPTARTYGPFRLYKKMP
                     SILRIMEETYQMPWLSEISLLALRVQLPDLQEMTFEQKKKELQGLFQGRNKKLLTYLK
                     KRQQHFIYQLNFEKAGMLQKDIELVTYFIRRIQEQKQFLRTPSLTFSMPLAADESQKK
                     HYLVCYGQLAETMITSGDVSPDFYYEKKEAHLSLKRQLSKEEIDPVQILISYRKKLEK
                     EQFEIELLNKKEAEKQLN"
BASE COUNT         1556 a          763 c          939 g         1335 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa cat
//
LOCUS       DANKAZ010000143         4475 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000143 DANKAZ010000000
VERSION     DANKAZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            323..1054
                     /locus_tag="PWY67_002641"
     CDS             323..1054
                     /locus_tag="PWY67_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2058846.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="PWY67_002642"
     CDS             1199..3226
                     /locus_tag="PWY67_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2058847.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="PWY67_002643"
     CDS             3334..4431
                     /locus_tag="PWY67_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2058848.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DANKAZ010000144         4432 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000144 DANKAZ010000000
VERSION     DANKAZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(543..2048)
                     /locus_tag="PWY67_002644"
     CDS             complement(543..2048)
                     /locus_tag="PWY67_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2058849.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="PWY67_002645"
     CDS             complement(2045..2731)
                     /locus_tag="PWY67_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2058850.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="PWY67_002646"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="PWY67_002646"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL2058851.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1545 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat at
//
LOCUS       DANKAZ010000145         4425 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000145 DANKAZ010000000
VERSION     DANKAZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            690..1658
                     /locus_tag="PWY67_002647"
     CDS             690..1658
                     /locus_tag="PWY67_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2058852.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="PWY67_002648"
     CDS             1669..2484
                     /locus_tag="PWY67_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDL2058853.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="PWY67_002649"
     CDS             2552..3262
                     /locus_tag="PWY67_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2058854.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="PWY67_002650"
     CDS             3298..>4425
                     /locus_tag="PWY67_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL2058855.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DANKAZ010000146         4390 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000146 DANKAZ010000000
VERSION     DANKAZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..330)
                     /locus_tag="PWY67_002651"
     CDS             complement(<1..330)
                     /locus_tag="PWY67_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058856.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADL"
     gene            complement(538..1464)
                     /gene="glsA"
                     /locus_tag="PWY67_002652"
     CDS             complement(538..1464)
                     /gene="glsA"
                     /locus_tag="PWY67_002652"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL2058857.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1487..2914)
                     /locus_tag="PWY67_002653"
     CDS             complement(1487..2914)
                     /locus_tag="PWY67_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2058858.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2945..4159)
                     /locus_tag="PWY67_002654"
     CDS             complement(2945..4159)
                     /locus_tag="PWY67_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL2058859.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1377 a          928 c          776 g         1309 t
ORIGIN      
        1 aagatcagcc agttctgtac tcgtgaatat accattaaaa taataactta aaaactttaa
       61 aaaataagat tgaccgtaca gaaactgtgt gagctcagaa agagcaatac catgtttgaa
      121 tacgagttta tacttatgct tctctcttac ttgttctttt atacaatatt ctagacactc
      181 ggctagactt tctagatcgt gaaggatcgt tggtgtagtc acccttagag aaatagctgt
      241 ttgttcgatg tctatttctt ggaattcaaa taattgttca cacagataca cttttctaaa
      301 tatatttcgc tcgatgtaat tttccagcat tcctgataca cgcctccagt ttttcttatg
      361 attttattta atcatatctg taaatcgtat ttttgtttcc tttttattta gaaaagccct
      421 ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa acaagctcac aatttcagcc
      481 tcattgtagg tagctttgag ccgtttggac ttttacccca gcctctttca agaatggtta
      541 ttcaaagatg tcagcgtcta gttctttgac tacatctttc aacaattgga taccagctgc
      601 gctgttacca aatggatcta gtgccgggct gaagacacct atgccgtaac gatttggcac
      661 agctgccatc aacccgccgc ctacaccgct ttttgcaggt acaccaacat ggacggaaaa
      721 ctctcctgat tcatcataca aaccagcagt tgtcataatt gatttgacga ttgtagcact
      781 ttcttcagta atgatttgtt tgccattcca aggagcaatt cctttatttg ccagcacagc
      841 agctaatttt gctatgcctg tagcggtgac attgagggag cattgtctga aataagcatc
      901 gagtatctcc ggtacgtctc cttcgatcat ttgattacct ttcatatagt aagcaagaga
      961 gcgattgata tcacctgttc ttgattcaga atgatagatt tcttcattca atgtgatatt
     1021 aggatcatca cagatctctt tcataaatga caagattcgc tcaaattttt catctgcatc
     1081 tttaccggca atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag gattcattgg
     1141 atgtttgcga tgattgattt ccatattcat gatagaattg aaggcaaaac cggttggttc
     1201 cgtgttgatt gtttgaaaga ctttttctat tccgttgtct ttgattgcta ataacaatac
     1261 gggaactttt gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc cctcaatcag
     1321 ttgttttgta tccaaatcat aaatcgccat acctaattgt ttgggatcca cttcagctaa
     1381 tgctggaatg taatccgcca cttgtcctgt ttgataatag tggaaatttt ttccaatcaa
     1441 ttgttctaat tcttttactt ccatttgaat aaccagcttt cattttttat ttttctgcat
     1501 gctggaattt atgacggcat gcgtagatcg taaatggaat aactaagacg ataagataag
     1561 aaacaatcag aatcgtttca taaatcccat gctgtccagc tgcgatacta gcaggtggaa
     1621 caaatgagat acaaaaagca aaaagtgaag tcaaaagacc taaacaagca acgattactt
     1681 ttacaacttt tccgcctgga acctgatagg ctctttcaag actgtcatct ttaaaaacca
     1741 gcttcaaata gcccgcgaaa aacataaagt acaacatcag gtagattaca acagtcaatg
     1801 acatcgctgc aagaaatgaa agattattcc ccccgccgct taatgtaaag acggctgccc
     1861 agattgttgc aacgatccca tttgcgatca tcaaatgagc aggaacaccg tgtgtgtttt
     1921 cttttgcaaa atattcagga agtaatcctt cttctgctgc tacgtgaagt gcttttgaag
     1981 gagaggttac ccaagaagca atttctccga tgacacctaa acaaacaaga atcgcaagaa
     2041 ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa taggaattct aacgattgaa
     2101 tcacccctgt attaagagac aaaccatgtt gaggtactgt agtagcgacg gtcaatccgc
     2161 caaaagtatc taaaatgatt gccagaataa ccaatacaaa cattgctaat ggataatcct
     2221 ttttagggtt ttccatttca tttgcatgac tggcagatgt ttctacgccc atatatgcta
     2281 aaataaagga aacaaaaaca accagcgtag gcaatttcga aaaatcagga atcaatgttt
     2341 tcatagacag tatcgtttgt agtggagcgc ctgaagcgac ataatggatc cctaagccaa
     2401 acaagactaa tgctggcaac aagataccag ctacaaaacc gatccgagca atcaatgaag
     2461 tgtatttggt tccttttaac tgtgagagta aaatagccca gaaaataatc aataggattg
     2521 ccaatttcag atatttattg ttttgtatag caccaatacc cgttgcatag gataatgcac
     2581 cagttaagaa atacagcatg gtcaagtagc caactgttat ctcaaaccat tggaaaaaga
     2641 ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac ccaagtatag acaccgcctt
     2701 tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa tgccacaggg ataaaccata
     2761 gcaaaccgcc taataacaag aaaaatacta agctgaagcc tgaggtcgcg aatgtgggat
     2821 attcatataa tgaaacgacc attgagatgg tcatagccaa aaagccaaag aacgttaatt
     2881 tttttgcagg aattttcgtt ttgctttgtt gcatgaatct tcctcttttc tatttgaatt
     2941 tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa tccttcttct tgttcttctt
     3001 ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa aatcactgtt gtcagcacat
     3061 aggcatacac ggcagtaatg atgacaccaa tcacttgaat ccctaagaag taccagctgc
     3121 ttggattcgt tagtaaatgg ctgttagatg caaagatccc tatcagaatc gtacctgtga
     3181 atccgcccat gccatgaaca ccccacacat caagtgcatc atcccattgt ttctttttac
     3241 gaaaatcaac agcagcatga cagacgatac ccgcaatgat cccaatcagc atggcacttt
     3301 ttgcttctac ataaccggca caaggcgtaa tggctgccaa tccagcaacg gatcctgtta
     3361 gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc aatgatcagc caagtaatca
     3421 tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt gactgcttgt tcatctgctc
     3481 gaagtgcacc accggaatta aaaccgaacc agccaaacca aagcagtcct gtaccgatag
     3541 cagctgccat aagactgcta tgctgacttc tttctcctaa aggaatcttt ctttgaccaa
     3601 gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt gtggatgact gctccaccag
     3661 cgaaatctct aaatcccatc tgatccaaaa atccgccacc ccatatccag tggcaaacag
     3721 ggaaataaat cagcagattc caaaaaataa caagtataat atagcctttt aaattcaatc
     3781 tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac gataaacatc agttggtaaa
     3841 gaaagaacat taaaaatgga atcgttgcac catgaaacat gttggggaag cttgtaacat
     3901 gtctcatcaa aaagaaatct gccgggtttc caataacacc gccgatatcc cgtccaaagg
     3961 ccaatccgaa tccaccaaat acccacataa tcgttgttat cccaacggag agaaaagatt
     4021 gagacatgat cgttagtata ttattcttcc gtgctaatcc gccgtaaaaa aaagctagac
     4081 caggagtcat caggcaaacc attgctgtac ataaaatcat gaatgctgta tctcctgtat
     4141 tgataggtaa tgtattcatt tttttcgcct cctttctacg attacgagta taaaagaagg
     4201 tgtagttgtt tttttcttct gtttttttga attaatactt gcatttataa aagacagaca
     4261 attaataaaa gcttgatgtg ttgtgctctt tttcgttttt cacacaaata aagacaaaaa
     4321 ataaacggtc ttctttttat agaaaaccgt ttattttcgt ctttttttat ttttaaagat
     4381 attttttcat
//
LOCUS       DANKAZ010000147         4379 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000147 DANKAZ010000000
VERSION     DANKAZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            89..559
                     /locus_tag="PWY67_002655"
     CDS             89..559
                     /locus_tag="PWY67_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL2058860.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            644..1252
                     /locus_tag="PWY67_002656"
     CDS             644..1252
                     /locus_tag="PWY67_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL2058861.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            1386..1586
                     /locus_tag="PWY67_002657"
     CDS             1386..1586
                     /locus_tag="PWY67_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2058862.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            2340..3566
                     /locus_tag="PWY67_002658"
     CDS             2340..3566
                     /locus_tag="PWY67_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2058863.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(3674..4144)
                     /locus_tag="PWY67_002659"
     CDS             complement(3674..4144)
                     /locus_tag="PWY67_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL2058864.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         1535 a          642 c          855 g         1347 t
ORIGIN      
        1 aaaaaaaatt aattttttta aataaagatg aaagttattg aaatatagag tatgataaat
       61 aagaattttt aataaggtag gttggctcat gaagcagtat cgacagttaa aaggatattt
      121 agaagcaaaa ggatattggg agatactagt ctatctcttt tttggtggtt tggctacgat
      181 tgtaaatttt gttagttttg ctctcgcacg ccaatatttt gatttaagta tggccttatc
      241 caattctatt tcgtggtttt gttctgtatt gtttgctttt gtaacgaaca aactttgggt
      301 attccattca aaatcaccga attttacaca tgcacttatt gaatgcggta agttcttctt
      361 ctaccgtatc ctttcttacg gattggacat gggggcaatg gtattgttga tcaatgttat
      421 gaacagcaat gagtatgtgg cgaaaatcat cactcaaatt attgttatcc ttgcgaacta
      481 tattttcagc aagttgttta tttttaaaga aactgaggtt tttgaagaag aagtaggtca
      541 aagtgaaaaa gacgactagg cataacaatt gaagaatttc cttctctttg ttactctttg
      601 taatgtagac attattaaga aaacattgag gaggaatttt tttatgactt acacattacc
      661 agatctacct tatgcatatg acgcattgga accttatatt gacgaagaaa cgatgcatct
      721 gcatcatgat aagcatcaca atacttatgt gacgaattta aattcagcaa ttgagaaata
      781 cccagaatta ggcgaaaaaa caatagaaga attattatct gatatggacg ctattccaac
      841 agatatcaag acagctgtac gtaacaatgg tggcggacat gctaaccatt catttttctg
      901 ggaaatcatg gcaccaaatg ctggtggcga acctacagga gaaataaaag aagcgattaa
      961 tgaagctttt ggtgattttt cttcttttaa agaagaattc aaaaaagcag ccgctggacg
     1021 atttggttct ggatgggctt ggcttgtaat ggaaaatgga aaattagcta ttacctctac
     1081 tgcaaatcaa gattctccat tgatggaagg aaagacacca attctaggtt tggacgtttg
     1141 ggaacatgct tattatttga aatataaaaa tgtccgacca gattatattg cagcattctg
     1201 gaatgttatt aattgggatg aagtaaacaa acgtttcgaa gcagcaaaat aaccatttcc
     1261 tccgtcatat cagtcttaaa aataatttgg aaaataaaag agcgaaagct tgctagcgtt
     1321 ttctaaattg ggtataatgt tcttgcaagg ttacttgcaa gaactttttt ggaggatata
     1381 catttatgga acacggaacg gttaaatggt tcaacaatga aaaaggattt gggtttatta
     1441 cagtagacgg cggagatgac gtatttgttc atttctcagc aattcaagga gacggcttca
     1501 agagcctaga agaaggtcaa gaagtagaat tcacgattgt tgaaggtgca cgcggacctc
     1561 aagctgctga agtgactaaa ttataataat ttgttgaata aagacacgac ttcttagaga
     1621 gtcgtgtctt tttgtctatt tatgaagttt ttagattttt tggctaagta aatgcatttg
     1681 ttcacttgac atgtcctaac taattatcaa gcaaggaaca ttttcttcgt tagtaatgga
     1741 ttttaaagaa atttcagttt ttggatgttg aataaaagac catttgtgaa accactagta
     1801 tacatactca ttaccatgtc aattctatta ctttaaaaga aaatcgtttt actttgaaga
     1861 ggagcatatc tatccattaa aagatgatgg taatgcaatc actagttcga gtcattttaa
     1921 gagccatgca gtgagctgga aataaaacga atagaagatg ggtttaacaa aaggaccagt
     1981 cataggaaga aattgtcgtg aaatcgaatg ataatctgaa cggtaggcaa agactgttaa
     2041 aaagatggga aaggtatgtg cgatcatttt actgcacgat tatttggaaa ataccttgtt
     2101 gttcgctttt tctggaaaaa atacataaaa aaagataaaa cgattgcgaa ctgcgtgtat
     2161 tcggtctttc gatctttttc acggtatggt atgatagtta agaattttga gaaattttaa
     2221 aaaataataa tcgttacaca aaaatgtttt ttaaagtttt cttatgatta aaaagtgaat
     2281 ttgctgattg aacagttata tgagacaata ttttagaaaa tgtgaaacag aggtgtatta
     2341 tgaagcgatg gcaaaaagtt ctattaacct tgttaggggt attcgttttg ctgattgccg
     2401 gattatcagc atacggaatc agatttatgg gagaagcgag ccaaacagta aacaaaattt
     2461 ctaaaaactc taatcgaata tcctccaaac gttctgagag agtaagcatt gatgacaaag
     2521 aacctttttc ggttttgcta ttaggtttag ataccggtgg attaggaaga acggaacaag
     2581 gacgttctga tacgatgatg gtagtaacag ttaatccgaa acaaaaaaaa tcaacgatca
     2641 tcagtttgga ccgagatatt tatacgaata tcgtaggtta tggaacggta gataaattaa
     2701 atcatgccta cgcatttggt ggtgtcgaaa tggccatgga ttcaattgag caactgctcg
     2761 atattcctat tgatcattat gtaacgatca atttggatgg catggaagat ttgatcaatg
     2821 cggtcggtgg catcaaagtg aataataaaa ttgactttac tttggatggt gtccatgtac
     2881 cagtaggtga acaaacattg aatggagaaa aaggtttagc ctattctcga atgcgacacg
     2941 aagatccaga aggcgatatc ggtcgacaac gtcgtcagcg ggaagtagtg acgaaaatcg
     3001 tcaataaagt tttaagttta gatggcgtaa gcaattatag aaaaatttta aaagcagttg
     3061 agaacaatgt gacgacggac ttagattggg atgacatgct tgatgtagcg acgaactaca
     3121 ccccggcgtt tgaaacaatc aaacaagacc agttgcaagg cgaaggacaa atgatcaatg
     3181 atatctacta tcaaatttta ggaaaaaatg atcttctttc gatccaaaat gaactgaaga
     3241 aacaattaaa tataaaaaca agcgatacat tacctaattt gaaaaatgag aatgcttcaa
     3301 tgatgttctt tgatgattcg gaagatggcg attcgacaaa cggtgatgga tcgaatagtg
     3361 aagggtacac cgattattct tcctattcag aaaacacata tgatcagtac caagatccga
     3421 atacctatta tcagcaaaca gaagatacaa gtcagtacta tacaccaagt caagatactc
     3481 aaagttatga tccttacgct ggacaggaac aaaatggtta tggttatgac cagaataatg
     3541 agtatggaac aacaagtggg tactagaaaa aaatagtttg gttaataaag agtgccacct
     3601 aaattagaca aaaaatctaa cttaggtggc actttttcta tgtgttaaat tacaggacgt
     3661 ctctttttag cgcttagttt aaaaaataaa ttgaataatt caatagagtt gcgaatagta
     3721 cccataaata ataaggaatc atgagccaca ttgtcaggcg atgatgcagc cacaactgat
     3781 acataagcac tgacaagaaa ataactaaaa gtgtaatatc gattgctgca atgaaaatat
     3841 ttcttaaatt aaaaaataga gcagaccata taaaattaaa aatgaactga atcacaaaaa
     3901 gaccgacaag attgtttcgc tgttttttgt tgttgtgatt aagtaacaaa taaagagaaa
     3961 tacccatcaa tatatacagt aaggtccaca tcggcccaaa tatccagcta ggtggtgcaa
     4021 atggtggaag ttgcaaagaa tagtaatatt ttccgggatt tccgctaagc aaccctgata
     4081 aaaagcctaa tatgacaatt cctataacac aaacccaaaa acgaatatct tttatcatct
     4141 tcattttact aactcctttc tataatatta gtttaccatg aaggctcaca gcaagacctg
     4201 tatttagttt gtgaagtaag aaaaaaattg ataattgttg ttattaaaaa gttgaacatt
     4261 tgtttatatg aatgatgttt ttgaaaaaga tcatatgatt tgattctcat gttttctatt
     4321 tgtacttttc tcgtctactt ctgttataat ctgcatctta cttttttcac cttttttac
//
LOCUS       DANKAZ010000148         4324 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000148 DANKAZ010000000
VERSION     DANKAZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..281)
                     /locus_tag="PWY67_002660"
     CDS             complement(<1..281)
                     /locus_tag="PWY67_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2058865.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="PWY67_002661"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="PWY67_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="PWY67_002662"
     CDS             complement(2359..2721)
                     /locus_tag="PWY67_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058866.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3063)
                     /locus_tag="PWY67_002663"
     CDS             complement(2725..3063)
                     /locus_tag="PWY67_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058867.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3042..3461)
                     /locus_tag="PWY67_002664"
     CDS             complement(3042..3461)
                     /locus_tag="PWY67_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058868.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="PWY67_002665"
     CDS             complement(3454..3687)
                     /locus_tag="PWY67_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058869.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="PWY67_002666"
     CDS             complement(3684..>4324)
                     /locus_tag="PWY67_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2058870.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DANKAZ010000149         4310 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000149 DANKAZ010000000
VERSION     DANKAZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(65..1285)
                     /locus_tag="PWY67_002667"
     CDS             complement(65..1285)
                     /locus_tag="PWY67_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL2058871.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="PWY67_002668"
     CDS             complement(1305..1916)
                     /locus_tag="PWY67_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2058872.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="PWY67_002669"
     CDS             complement(1959..2891)
                     /locus_tag="PWY67_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL2058873.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="PWY67_002670"
     CDS             complement(3312..4172)
                     /locus_tag="PWY67_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2058874.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DANKAZ010000150         4251 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000150 DANKAZ010000000
VERSION     DANKAZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            577..909
                     /locus_tag="PWY67_002671"
                     /pseudo
     CDS             577..909
                     /locus_tag="PWY67_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            923..1072
                     /locus_tag="PWY67_002672"
     CDS             923..1072
                     /locus_tag="PWY67_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058875.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1069..1515
                     /locus_tag="PWY67_002673"
     CDS             1069..1515
                     /locus_tag="PWY67_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058876.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="PWY67_002674"
     CDS             1517..1654
                     /locus_tag="PWY67_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2058877.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="PWY67_002675"
     CDS             1691..1984
                     /locus_tag="PWY67_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058878.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="PWY67_002676"
     CDS             1981..2205
                     /locus_tag="PWY67_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2058879.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="PWY67_002677"
     CDS             2202..3221
                     /locus_tag="PWY67_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2058880.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            3410..3483
                     /locus_tag="PWY67_002678"
     tRNA            3410..3483
                     /locus_tag="PWY67_002678"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3444..3446,aa:Ile,seq:cat)
BASE COUNT         1466 a          750 c          852 g         1183 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
     3421 tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt
     3481 acattaacgt agccatttga atcgttctgt gttagaattt ttttgaagag tattatacaa
     3541 gctaaagctt ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga
     3601 ttaatgaaag gatgtttcac atagttatac ttctgtatat ttgaaaagtt ttactttgat
     3661 ttttaaatag aaagacattt gggttaaatt gtgagataat aataaagaag agtttaaagc
     3721 gcaccccaaa ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagcc
     3781 attaaaaagc ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg
     3841 tagttaatta gtgacttatt tttgatttta tagcactgat actataaaat atagatatca
     3901 tcatattaca caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata
     3961 aaattcgttc cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc
     4021 aacgaaagta tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc
     4081 agtcatgaat cggctacact agactagaca gaaaaaataa ggtgtgtaga ataataaaaa
     4141 acacactgga ggatttttca tgtcaagacg tcaacgaaga acctattcaa aagaattcaa
     4201 acaacaaatc gtcgatctct atctcgctgg taagcctcgc gcagaaatta t
//
LOCUS       DANKAZ010000151         4174 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000151 DANKAZ010000000
VERSION     DANKAZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(24..1790)
                     /locus_tag="PWY67_002679"
     CDS             complement(24..1790)
                     /locus_tag="PWY67_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2058881.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1750..3503)
                     /locus_tag="PWY67_002680"
                     /pseudo
     CDS             complement(1750..3503)
                     /locus_tag="PWY67_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3613..>4174)
                     /locus_tag="PWY67_002681"
     CDS             complement(3613..>4174)
                     /locus_tag="PWY67_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDL2058882.1"
                     /translation="KDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSG
                     IIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYD
                     RAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGI
                     ISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1410 a          793 c          589 g         1382 t
ORIGIN      
        1 aggttaattt aaagcctatt tcatcatcgt gattgagctt ctttttcata gacgaatttc
       61 tcagatagtt tagttttctt tatgagattt ttgtatattt ctgaccttcg atacaactca
      121 ttatgagtac cttgatcaac tagttttcct tgatccatca ctacaatttt atttaccttt
      181 tcaatagact tcaagcgatg cgatataatt agtactgtct tatcttgtat taaacgattt
      241 aaactttcct gaatacgttt ttcattttcc gcatctaaac tggcagtaat ttcatctaaa
      301 attaaaattg gcgcattttt caaaaaagcc cgtgctatgg ataggcgttg tcgctctcct
      361 ccagaaagtg aacttccgtt ttctccgacc aatgtatgat aaccgtcagg tagtttttca
      421 ataaagtctt cgcagttagc taaacgagct gcttttttca cttcctcatc tgacgcttga
      481 ctatttccaa tacgtatatt ttccaaaata cttgtattaa acaaagttac ctcttgaaaa
      541 acaatagcga ttttgctaaa caaagaagct ggagatacac gtttaatatc atacccatca
      601 attaaaatac agccttcatc ataatcatac agtctagaca ctaatttcaa gatactagtt
      661 ttcccggaac cactagcacc tactaacgca gtcacttctc cttgtttagc cgtaaaagag
      721 atatgatcta aaattggggt attgttatca tatgagaaag agacatctcg caactcaatg
      781 tcaaaagatt tcagaggact atctgatccc tcttggatac tggtttcctt catagctcta
      841 attcgctgta ttttaggagc taaataaaat atttcaagca ctgcttcttt catagaatcg
      901 aaggaatcct ttatttttat tgcggctagt aagtaaccaa cgacgtagag tatagtcact
      961 tcacccgtta acagtaagtg aacacctact agaatgacta ctgctaaact aacatatgag
     1021 aaaatagagg ataaggccat gactgaaaaa gtacttaatt ctacctttaa atgaatcctt
     1081 tcactctctt ccattttttt gaaaagtctg tcttgaactt ttttagatag attaaagcta
     1141 ttaatctctt gctgcaattc tatagtttct tgaaattctt ccgagttttc tctcaaagta
     1201 tcgtaatatt tagtattggc tttcgtttgg gatttctttg ataacaagat taagacaaaa
     1261 ctaaataacg ttggcaaaat aacagctaat cccattttga cattaccaac caaaagcatc
     1321 actgaaataa aagggaaaaa cagagccata ccgccggctt taggtattgc atgactcatc
     1381 gcatgctcaa taccttcaac gtcagacatg atagtttgag ataaatctga taaattatgt
     1441 tttgaaaaat aggataatgg taaatttgac aaatcgtctg caatttgcac tcttaaatgc
     1501 gcactttctt tataagtact attatataag ttttcatact ccctatctaa caaccaatac
     1561 atgaaaagca aagtaacaaa cgatacggca aaataaagcc aacggggctt tgtattttct
     1621 aaaactaatt cgtctatcag catcattata atgaatgctg gtaccatatt aatgcagtat
     1681 acaaaaaaac tagaaataga agctttagtt aaagcttctg cgccctgatc acttaaagca
     1741 aatttgtttt tataaaaact tttcattgtc aagcctccaa tcatttgcat tctcataatt
     1801 agtgaccaat tgtttataca cttgatgttt agccattaat tcattatgat ttccacgttc
     1861 tactattttc ccttctgaca agacaagaat ttcatctaca gatttaatgc tggataatcg
     1921 atgagctatc ataataactg ttttttcttt catcagatgt tgaaaagcct tttgtagttc
     1981 atactcatta tctggatcaa tagatgcact ggcttcatcc ataataacaa tctttgcatc
     2041 ttttaaaatt gctctggcaa tagctatacg ttgtttttct cctcctgaca gataaactcc
     2101 tttactacca attaaggtat gctctctttc gggaaatttt tctaaaatcg attcgcaacc
     2161 ggctaatctt agcgcgtcaa atactttact tgctggcgct tgaggatttg ctatagaaac
     2221 gttatcgtaa atactctgat tgaatagttt cgtatcttga aagacaaaag caattgtgtt
     2281 aataattgtg tcttttgaat agtgatttaa aggcttacca ccaattttaa tctgtccctt
     2341 atccacattg tagaaacctg aaattagttt agctatcgta ctctttccgc ttcctgattt
     2401 tcctactaat gcataaattc ttttttgttc taaaactaag ttgaaatttt ctaaaacaca
     2461 ctcttctttg tatgaaaagc tgacattttc aaatgcaatg tcacaatttt taatctgtgt
     2521 ctcgttccca tattttagtt ggtcttgctt catttctaaa taaagggagt ctagtgtttc
     2581 gactgcatat cttgcttcaa acaagtgcat acttacatac attattctca tgaaagaaac
     2641 aaaaataatt ccacttaaaa aaaacaacat caacaagtca acagctaata tcttgggagt
     2701 ggtaagagaa gttataaaaa aggttatagg aactatcaaa atgcaaacta tgccaaaaaa
     2761 tagccattga aaaagaacaa aaggtgtttt cccactcaaa gaatacttat atgcaaactt
     2821 agcataatcg ttaattgctt tattcaaggc tttaaatgat gttactttag caccaaatat
     2881 ctttataact tgaattccgc ggatatattc taccgtttca gagcttaaca catctaacga
     2941 ttcttgatat ttttgaataa aatttgtatt ctctgtcatt ttctttaaca agaatacacc
     3001 aattactaac atggcgatta gaactaatcc aattttaaag ttaatgaaaa agcctaaggc
     3061 aactgataaa acaggtgtta ctatggcttg agcactatct ggaatcaagt gagcaattgc
     3121 catatgtgtt ttagccgcgt tgtcatcaat tgtctttcga atatatcccg aagaatttaa
     3181 atcaaaaaaa cgaaaacttg cttctgttaa accgctaatt cccttttttc tcaaattagt
     3241 ctctaagcga aacccaagga tatgagaaaa taagccagaa aaaatataaa ataaagctct
     3301 aaagttaacc aagcaacagt ttgaaccgca taactcttag ctaaatattc atctcctaca
     3361 ataattagag actgaagaaa tttgtaaata ctgtaatatc catagactgt taatacgaca
     3421 gaaaaatcag aaaataaagt ggctaaccat ccgtagtatt tcattttagg gacataacta
     3481 aataattttt tgtatacttt cattttttcc tcccccgcat tcaatttctt ctcttattcg
     3541 tttacaactg ttaatgcttt ccgtaatagc gtaaattaga gatgtacaaa cagataaaag
     3601 gtaaactacg ttctattttt tgtatttttt taagactttt cttccaatca atacaccaat
     3661 catagataaa cttgctacaa ctaacactga aatgattccc catataccta ccgcatagtg
     3721 gatcattgca tccattgttg cagcagattg tcctttatct atccaatctt gacgaaaacg
     3781 atctgtaaag aaccatatag gaatccactg tccggcagca ctgcctaatt gcattagagc
     3841 ccaagacgca acttgaggaa ttgctcgatc atatccaaat acagatagaa taatatcagc
     3901 taagatcgcc atactaatcg aactgataaa ccaaggtaag tacgccccgc ccattaacgt
     3961 aaaaaataac aataaaacaa ttgtgtaaat agtaaaaatt ccttttctag ggtagttata
     4021 gctaatataa acaaatatga ttccagataa taatccaaaa atcccggggg atatgctgtg
     4081 accaaacgcc ccaccaatac tactaaccat tattgaaaaa aagtaaatta ctagggaaat
     4141 aactgtagct aaaccaataa aaatataatc tttt
//
LOCUS       DANKAZ010000152         4152 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000152 DANKAZ010000000
VERSION     DANKAZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..106
                     /locus_tag="PWY67_002682"
     CDS             <1..106
                     /locus_tag="PWY67_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2058883.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="PWY67_002683"
     CDS             complement(359..508)
                     /locus_tag="PWY67_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058884.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="PWY67_002684"
     CDS             970..1182
                     /locus_tag="PWY67_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058885.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="PWY67_002685"
     CDS             complement(1460..3259)
                     /locus_tag="PWY67_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2058886.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="PWY67_002686"
     CDS             complement(3383..3979)
                     /locus_tag="PWY67_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2058887.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1227 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt tt
//
LOCUS       DANKAZ010000153         4077 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000153 DANKAZ010000000
VERSION     DANKAZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(109..651)
                     /locus_tag="PWY67_002687"
     CDS             complement(109..651)
                     /locus_tag="PWY67_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL2058888.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            786..1175
                     /locus_tag="PWY67_002688"
     CDS             786..1175
                     /locus_tag="PWY67_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058889.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1179..1943
                     /locus_tag="PWY67_002689"
     CDS             1179..1943
                     /locus_tag="PWY67_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2058890.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2072..3019
                     /locus_tag="PWY67_002690"
     CDS             2072..3019
                     /locus_tag="PWY67_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2058891.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3149..3889
                     /locus_tag="PWY67_002691"
     CDS             3149..3889
                     /locus_tag="PWY67_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058892.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1359 a          690 c          820 g         1208 t
ORIGIN      
        1 taacaaattg atgagtatat gtcgatgttt tttaatcaaa ataataaata aaaactgtct
       61 aacatttaaa atttaattta gatattagac agtttttaag tttgtgagtt attttccttg
      121 tcgttttctc acttttttag tccagaaacc acctgaaata tattttggct cataagaaat
      181 gataaaagca cgctcttcga tttctttgat caggcgatac agttcacgtt cgctttttct
      241 aggagaaagt atttcgagaa ctagccgctc accttccagc cccattgcag aagtctgtgt
      301 cacaccataa ccgtgttctc tcagtatacc cggcaaactt ttatcttctg cgctatttgc
      361 tggcaaaatc gttgtgacca tgatgtatcc taatgccaat ttgtcttcaa tcaaaattcc
      421 gacactgata cctactgcgt aacctaacgc atagacaaat aaattcagtg gattatctaa
      481 acgatctaaa acaagggata agccgacgat ataaatcgtg atctcgacca tactgacaag
      541 tggagcaatc agtcgatagc ctttcattgt caacatgaaa cgtaacgtgt tcagagtgat
      601 ataagcaaaa ttaatgagga agatcatccc caacattttt ggatcaatca tttgtaaaac
      661 tcctttcatt tatataacaa caatatcaaa aaaaatcgta agtgaagaaa aattgaaact
      721 ttatacatac aaaaaaatga taaaatgatt ataatagaat tataaagata ggttaggtga
      781 ttaaaatgaa aacggtcaat cgtgtgtttt gtgccagcca cgtgctaagt ctccttctca
      841 gtatatactt aggtgtctat tcgattcaac agccaattga ggtagagaac attgtttttc
      901 agatgtcgct gacagatggt ttaattttaa gcgttttttt ctttttgata ctgtttattg
      961 gtaatatttt cggtagcgtt gccagttttt tgaatttttc tatttatcca ctcctttcgc
     1021 tagcattagg agtggtggga ctttgttctc tgtgcttatt tcccgagcca ttcagtcctg
     1081 catttatatt atttggtaca ctgaacctgt tccagacaac tgttggagca tggttgctat
     1141 ggcgatcagg aaatttaatg aaaataggtg agtaacaatt gaatccaatt atattgaaac
     1201 gttctgtcca tcatattcca gtgttagaag ttgttctgaa agaattaaga gaggaacgta
     1261 ttcccgttgt catctattat cacggatggc agacaagtaa ggagttagtt ttgacgcaag
     1321 gaagaaaaat tgcgcagcag ggcattcgtg tgatcctgcc agatgcgatg aatcatgggg
     1381 agcgcaaaca gccggtttct tctattccat cctatacatt ttgggatagt atctacggaa
     1441 atctgtttga atttgacaca cttatcaagc atttagaaaa aagaggtctt ctacatgatc
     1501 agcttgcagt tggcggggtt tcgatggggg gaatgacaac atgcgccttg ttggctaaac
     1561 atcctcatat agtagcgggc gtgtgtttga tgggttcccc tgcacctttg gaatatggaa
     1621 aagaaatcgt aaggcgcgca tcggaatata attatgtcgt accaaaagac tattttgatt
     1681 tgatttcatg ggttaagagc tatgatttgt cattgcagcc ggaaaagtta gctggacgtc
     1741 ctctgttctt ttggcatgga caagctgacg aaaaaatccc ttatatacaa acggaacgat
     1801 ttgtagaaaa aaatcggcat aagtcttacg gaaaagaaat tgtttttcgt tcctcaaaaa
     1861 cacagcgaca tatggtacaa ataccactaa tggaagaagc ttccactttt ttagcaaaaa
     1921 atttgataaa aaagtataaa taattgaaac ggaaaatcac tcatgacgat agaggatttt
     1981 ccgtttttta ttttgggcta taataaactc tgttaggaaa atttggtata attgatagta
     2041 agaatgacaa catgttagaa aggaagagag catgcccact ttagcagatg ttgcaaaacg
     2101 tgccaatgtt tcaaaaatga cggtctctcg agtcatcaat catccggaac aggtgactga
     2161 cgaattgaaa gagttagttt tctcagcaat ggctgaactg gattaccgtc caaatattgc
     2221 tgcaaaagca cttgtcacga atcggtcgca gatcatcaaa ttatttatac tggaagaaat
     2281 cgacacaact gagccctatt acatgaattt gctgatggga atcgctaaac ggatcggtaa
     2341 agcgcattat tctctccaat tagttacaaa tgatgccttc gacgtcggtt cttgtgatgg
     2401 atacatcatc acaggtgtca gaaaaaaaga cttcgattgg atcgaccgtt tggaaaaacc
     2461 agttgtcttg tttggggaaa attctgaagg atatgattat gtagacagcg ataatgaata
     2521 tggtacagca aaagcaacgc aatatgcctt agaaagagat tatcaaacga ttatttatat
     2581 cggtatcgaa gtggacgagc cttttgaaat ttcaagggaa actggttatt tatctgtgat
     2641 gaaggaagtt gatcttccta gtaaaatctt gcggtttcca aatcattcga cagaagtaga
     2701 aagatatatg acagaaaact gggcagattt tacagataaa acttgttttg tatgtagttc
     2761 agatcggcta gcactaggag cagtaagagg gattataaat gctggaggac acttgcccga
     2821 agaatttggt gtgattggat ttgatggggt atttttagac caaatcagtt cacctaaatt
     2881 gacaacgatc aagcaagata ttatacgtat gggtgaagta tgtggagaaa tgttgttaaa
     2941 aaaaatagaa gaaaaagggg cgagtcaagg ttatcgtcgt ttttttcctg aactagttat
     3001 cagagagaca acgaattaag tcttattact atatatatat ttatgaaatt catcgctatt
     3061 ttcttaaaat aatcagcgca ttgctactga aaacaatgaa tctgtgctat tattgagaaa
     3121 agttccattt ttgagaagga tggtttgaat gaaagaaatc agacgaaaac atttccgttt
     3181 tcctgcgtac gatgatgagt taggcgtaaa attaaccaac gacaagaagc gagtattatt
     3241 tgacggacaa gaagatcttt tgactgaaca gaatcagtcc ccgtttgaac agatgaaaga
     3301 atttcatttc acgaacacga acaccgagaa aatccgtcgc aagcgtcgtg caacaaatgg
     3361 attgaagtca gctccgcgta aggaaaattt ggaaaagcat aaaggaaaat taccggatta
     3421 taccggccat taccaatcag aaatcacatc gacaggacga aaaaaacttt ttggtgattc
     3481 ctctcgttct gtcagacaaa gtgaaacaag gacagagtcc aagcatacta cacgaaacgc
     3541 atctgttgac acgaaggtgc gggagcgttc ttattttgtt cctaaatatg tacccgcatc
     3601 gattatccca gaccctagag aaagcaaaat ttctgaggat catttaatcg aatcgatgaa
     3661 aaaaacggat gactcttatc tgttgttcga tacagaaccg acgccgtatc aagaaaaaag
     3721 aaatggtgag ccgtctgtac gaacattcaa tcgaacaaaa acgttagagg aaacaaagca
     3781 gtcagaagaa aaaaaatcga aaaaaagcca tggtatctta gaacgctcat tacaaggatt
     3841 gatcgatgat caaggaaact ctttgggaga aaatagctat tttaaataag tgatatacaa
     3901 atttatattc ggctacagtt ggaaaggaaa cgctgcggcg tttccttatt ttatactcta
     3961 tccgaaagtt tatatcgtat cctcaaattt ttttgagttt aagcgaattt tttctcccaa
     4021 taagtaagaa aataatggca aaataaaaaa acaaactatt ttcgcagatc gtttgca
//
LOCUS       DANKAZ010000154         4002 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000154 DANKAZ010000000
VERSION     DANKAZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..349
                     /locus_tag="PWY67_002692"
     CDS             <1..349
                     /locus_tag="PWY67_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HDL2058893.1"
                     /translation="VYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKV
                     TDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQR
                     GIKNLVKSYLKMN"
     gene            352..1248
                     /locus_tag="PWY67_002693"
     CDS             352..1248
                     /locus_tag="PWY67_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2058894.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1298..1687
                     /locus_tag="PWY67_002694"
     CDS             1298..1687
                     /locus_tag="PWY67_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2058895.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1674..>4002
                     /locus_tag="PWY67_002695"
     CDS             1674..>4002
                     /locus_tag="PWY67_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2058896.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1347 a          635 c          844 g         1176 t
ORIGIN      
        1 tgtttatgat gagtattcaa aagagacaga tatttatagg gaactgaaac gtgatgtgga
       61 ggcacttaag gaaattccca aaaatctaac tgatagtgtt gacaaaattg actttggcaa
      121 tggtataaaa gttacagacg aaattgatga acaccgcgga gaattgatta agaatttagt
      181 gaccaatcgg gctaagattc aaacaataat taatgaaatg tcagatgaaa ttgatagtta
      241 tagagcagag ctgaataaat cagattggaa agttaactat aatgaggttt caaagaggta
      301 tcaaagaggt atcaaaaact tagtgaagag ttatctcaag atgaattaaa aatgttacaa
      361 aaccttgatt ctttatatca atctttatca aaaactgaag agcgattgga agaaattatt
      421 caacaagaaa taaagcttaa agattaccaa gttgaggttg actcagcaat gtcaagaatg
      481 aataaatcat atcaaaatct ctattctgag agaagtagtt cctcgcgcga aattttttct
      541 gatattacag gaatagaggc tactattcaa ccaaataggg atttcaataa ttatgttaaa
      601 gagctcagag cttgtattaa taaggaggac acttttgaca gtgagtttgg atctcttatt
      661 gataagctga caaaaaattc cattactaat caagatatat ataatgacat acttgatata
      721 agagttagtg gggaaagtag tattttttca gataaaaaat taatcaattc tataagaaaa
      781 ttgaatgatg cacaagttct tgaattacaa acccttaagc caaatgattt agtagttatc
      841 cgacttgaac taaatggaaa aaaagttagt ttatcgaatg ctagtgctgg tcaaaagaca
      901 tctgcaattc tttctatgat tttggcgtat ggaaattcac cactcatatt ggatcagcct
      961 gaagatgatt tggatagtca attaattaat aacttaatcg taagaagtat cattacaaaa
     1021 aaggaaaaca gacaaatttt aattgtaact cataatgcaa atatcccagt taatggtgat
     1081 tcagaatggt tgattagcat gggagattca aaagaaatat cggctgatca gtgcgggtcg
     1141 gtagatgaac ccaatatcaa gccaagaata tgtactgtga tggaaggtgg cgaggatgct
     1201 tttacaaata gagcgaaacg ctatggtttt aaacaaattg tagaataaca ctagagggtg
     1261 agacatatta ttatgtccgc cctttttagg aggaaaaatg aaaaaaataa aaagctatac
     1321 cagtatctgg gcagttgaaa aagtcattta cgcaatcaat gattttcagt tgccttttcc
     1381 tgtgactttt aaccaaatgg cttggttcgt tttatcttta ctgtttgtca tcgtatttgc
     1441 ccatgtgcct ccactatcta tgatagagga ggtcttttta aaatacttag gcataccggt
     1501 ggctgtcact tggtttatgt cgcaaaagac gtttgatggc aagaaaccac tgggattttt
     1561 acgatccttt atcagctatc acttgcgatt taaggtgact tttgcaggaa agaaagtcaa
     1621 agaacagaag aaacgatggg atgaacccag tactcttgta aggagtgtga actatgtatc
     1681 cgattaagta tattgaaaat aatctcgtct ttaaccaaga aggcgagtgt tttgcctact
     1741 atgaattagt gccttacaat tactcctttt tgtcacccga acagaaattt caagtgcatg
     1801 ataactttcg ccaactaatt gcgcaaaatc gtgaaggcaa gatccatgcg ttacaaattg
     1861 ccacagaaag tagtattcgc gccacacaag aacgatcaaa aaaagaaatt accggtagac
     1921 tcaaagaagt agcgaaacaa cgaattgata tacagacaga tgcattagta tccatgattg
     1981 gcgatagtca gattgattat cgctttttta ttggctttaa actaattgcg acagatgaag
     2041 aagtcaatct aaagagtctc aagaaatctt tcttttctgg gcttcaagaa tttgtctatg
     2101 gagtgaatca tcatttgatg ggcgattttg tttccttatc caatgaagaa attcgctgct
     2161 attctaagct tgaaaaatta atggaaagca aattggctcg aagatttaaa gtcaggcgag
     2221 tcacaccgag tgacctgacg tatttgattg aacatattta tggcgaaaaa gggataccgt
     2281 ttgaagaata cgaatttcag ctaccaaaga aaaagctaaa atcagaaaca ctggtgaaac
     2341 gttatgactt attacgtcca aatcgttgtt taattgaaga aaaaccaagg tgcttacgga
     2401 tggaacatga gaatcatgag tcgtacgtag cgtatttaac aattaataca attgtggggg
     2461 agatggaatt tccttcctca gaacttttct attaccagca acaacaattt acattcccaa
     2521 tcgacacaag tatgaatgta gagattgtga cgaataaaaa ggctttagct accgttcgta
     2581 ataagaaaaa agaactgaaa gatttagaca atcatgctta ccaatctgac aatgaaacga
     2641 attccaatgt cttagatgct ttggattccg tggatgaatt ggaaaccacc ttggatcagt
     2701 caaaagaatc tatgtacaaa ctgagttatg tggttcgagt gagtgccgaa tcagtggatg
     2761 aactaaagcg acgctgtgat gaagtgctcg atttttatga tgataccaat gtaaaactag
     2821 tccggccctt tggcgatatg atggggctac atgaagaatt cttaccctca agtaaacgat
     2881 atatgaacga ctatattcag tatgttacgt ctgatttttt ggcggggctt ggttttgggg
     2941 ctactcagat gctaggagaa ttagaaggga tttactttgg ctacaatgtg gatacaggtc
     3001 gtaatgttta cctgaaaccg gcattggctt cacaaggagt caagggttca gtaaccaatg
     3061 cgctggccgc cgctttcctg ggttctcttg gtggaggtaa atcctttagt aacaatctct
     3121 tagtctacta tgctgttttg ttcggtggtc aagctgtgat tgtcgatcca aaaggggagc
     3181 gaggagggtg gaaagaaacc ttgccagaaa ttgcggatga aatcagtatc ctcaacttga
     3241 cgagtaagtc agaaaatcaa ggtctgcttg atccttatgt gattatgaag aaaaagaaag
     3301 actcggaaag tttagcaatt gatattctga cttttttaac aggaatttca agtcgagacg
     3361 gggagaagtt tcctgtgtta cgtaaagcca ttcgaacggt gactcaaagt gatcaacggg
     3421 ggttattaca agttattaac gaattgcgaa aagacgggtc accaatcgcg gaaaatatcg
     3481 ccgatcatat cgagtccttt accgattatg attttgctca tttgcttttc tcagacggct
     3541 cgattaccca atcgattagt ttagataaac agctaaatat cattcaagtg gcggatttgg
     3601 ttttaccgga tgcagaaacg acttttgaag aatataccac catggaattg cttagtgttt
     3661 cgatgctaat cgtcatttcc acttttgcct tggactttat ccatagtaac cgagaaatct
     3721 tcaaaattgt cgatttggat gaggcttgga gttttcttca agtggctcaa ggaaaaacgt
     3781 tatcgaataa gcttgttcgt gctggtcggg ccatgaatgc tggggtctat ttcgtcactc
     3841 aaaatagcga cgatttatta gatgaacgtt taaaaaataa tatcgggttg aagtttgctt
     3901 ttcgcagtac cgatattcat gaaatcaaga aaaccttgga attctttggt ttggatcagg
     3961 aggatcaaag caatcaaaaa cgcctgcgag atttggaaaa cg
//
LOCUS       DANKAZ010000155         3998 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000155 DANKAZ010000000
VERSION     DANKAZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..544
                     /locus_tag="PWY67_002696"
     CDS             <1..544
                     /locus_tag="PWY67_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDL2058897.1"
                     /translation="EKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLP
                     EVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSET
                     KLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKY
                     AGVSPKQYQLGLVDNFNNIS"
     gene            561..995
                     /locus_tag="PWY67_002697"
     CDS             561..995
                     /locus_tag="PWY67_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058898.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1612..>3998
                     /gene="esp"
                     /locus_tag="PWY67_002698"
     CDS             1612..>3998
                     /gene="esp"
                     /locus_tag="PWY67_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDL2058899.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT         1463 a          562 c          767 g         1206 t
ORIGIN      
        1 tgaaaaaaat tattctatca ttgtttttga aaagttagca caaacgtcta taacattagg
       61 aatggatata atagaagcgt atcaaagtag agatgctttg atacaagaaa acgaattagc
      121 tgtatcgtta cctgaggtct taaaagtgag ggattctggg attgtttatt atactaagga
      181 aatcggaaaa actaagatag agcatctttc tcccttaatt tcttctgtcg ttcaattcat
      241 cggcttaaat atctataaaa gaattactgt aaaggaaata gctaattatt tttctgtaag
      301 tgaaactaaa ttaagagaaa gctttaagaa tgaaatgcat attacgattt ataattatat
      361 ctctaagcga aaaatttcta cggctaaaat aatgttgaaa tcaaaccata ctatcagtga
      421 agtttcttta ggtttaggat tttcagattc ttcacacttt tcgagagtat tcaaaaagta
      481 cgcaggtgta tctcctaagc aatatcaatt aggtttagtg gacaatttca acaacattag
      541 ctgagaggtt ttattttatt atgagactat atcatttacg tagtttggag gagttgagga
      601 aattggattt taacaaaaaa tttttttggg aaatttctga tttgagttgt ttcgtagaat
      661 ctgagcgtct aagtaatggt aataatagtg tgttaaatga atatgacaaa gtagtaatta
      721 ataaagtaaa aagtttatgt gaagaagcta gagaatctat cagtcatatt aatggtagca
      781 attttaaaca aacattattt aatctgatta agctagatgc taaaatatca agttatttat
      841 tctttttgat acatgatgaa tttatggatt atcaaaaatt ggatcagtta attgaaagag
      901 aaagctggga agattattac gtgggtctaa ctttttcaga aaagttaaac aactacaagt
      961 taatagatta taagtatttg agtgaaagca attaattatt catgtagagt actagacact
     1021 ttaaatatgg acaattcaat tataaagaaa aagaatcaat aatttaatga ggctgtgaca
     1081 aatgtcattt tttgtttcgc taaaaacgag tgaaaaaatg ttgataactc caatcattcg
     1141 tttaagattc taggaatcat tcagaaatgc tgtgaaaccg atactttata attataaaga
     1201 aggtgatttc aaattccgaa gaaggaattt gaagtcacct ttttacttat gtcacagtct
     1261 cattcagtaa tgcgggataa ggctccttat tccgttattt gaatactctg aagtttaatc
     1321 taagaaaggg taatattttg aaaacatgct tcatcgcata gaaatgaact taatttcaga
     1381 aagaatacat acatatcttt ttatatgttt tcagctgctg aatggttaaa aagcacaaat
     1441 atccgaaaaa aacatgaaga aatgtacttt ttttgttgta atgttcatga aattctctaa
     1501 gtaaattagt taacataagg ttagtaagat tcctattctg tgaataatac gtatcagatt
     1561 tatcttttat tcttactagg aaagtaatta ttaagggagt tgatttgaat aatggttagc
     1621 aagaataata agagagtatt tctagaaaaa acaaagaaac gagtattgaa gtacagtatc
     1681 aaaaaattaa gtgttggggt tgcatctgtt ttgattggtg ttggtcttgt cttgggaaca
     1741 actgaactag ttaaagcaca agatgaaatt tctccaagca gcccacttga aacagcgata
     1801 agctccgtac aagtaggtga caaagtagct tcggggaata cttttcagga gaatcctggt
     1861 tatacgaaaa attataactt ttcggattta caatttagtc ctcaagaact aactggagac
     1921 acactaaaag gcaatacaat tggatttgaa gtttacggaa aacataatat tgcagcatca
     1981 actaaaaact gggaaatccg tcttcaatta gatgaacgat tagctaaata tgtagaaaaa
     2041 attcaggtcg atccaaagaa aggaattgga tcttctagac ggacctttgt aagaattaat
     2101 gattcgctgg gtagacctac aaatatttgg aaggtcaatt atattcgtgc aagtgatgga
     2161 ttatttgctg gggcagaaac aaccgatacg caaaccgctc ctaatggggt aattacgttt
     2221 gaaaagagcc tagatgaaat ttttaaagag ataggcatag acaatctcaa gaccgatcgt
     2281 ttaatgtatc gaatctactt ggtaagtcat caagacgatg ataaaattgt accgggtatt
     2341 gatagtacgg gctacttttt aacagattca gatgatttct ataatagctt agatgtatct
     2401 gaaaataatc ctgaccagtt taaacatgga tcagtaagtg caaaatatga gaaacctaat
     2461 actcaaacaa aagatggttc aggatccaca ggtgctaatg gagctataat cttagatcat
     2521 aagttaacga aaaattataa tttctcatat tcatcttccg caaagggcac gccttggtat
     2581 gctaattaca aaattgatga acgattagtt ccatatgtag cgggtataca gatgcacatg
     2641 gttcaggctg acaaagtaac atatgatgtt tcgtttgaat ccggaacaaa agtagctgat
     2701 ttagcaatcg agcgacggga aggtcatgaa aattatggca taggttcaat aactaataat
     2761 gatttaacta aacttatcga ttttgctaat gcaagtccac gtccagtcgt tattcgatat
     2821 gttttacaat tgactaagcc attagatgaa atcttagaag atatgaaagc aacagcacaa
     2881 gttgaagaaa ataaaccatt tggtgaagat ttcatctttg attcttggtt gtcggatacg
     2941 aataaaaaat taatccagaa cacttatgga acaggttatt attatttgca agatattgat
     3001 ggtgatggaa accctgacga taaagaagag agcggagaca cgaatccata tatcgggaaa
     3061 cctgaattag aagaagtata tgatgttgac acaacagtta aggggaaagt attcatccac
     3121 gagttagcgg gaacaggtca caaagcccaa cttgttgata aagaaggtac tgtattagca
     3181 gaaaaaacta tcgctccaaa tgaaaaagat ggggctccaa tttcagatac tgtagaattt
     3241 gaatttacgg gtgtagattc aagtaaatta atcgcgaaag atgaattaaa aatccaaatc
     3301 gtttctccag gttttgataa accagaagaa ggttcaaccg ttattaagga atcaccaaaa
     3361 gcggttgata aacaaaccgt ggtagttgga tttaaaccag atgctaaaga atcaattcgg
     3421 aataataaaa acttacctga agatgcagag tattcatgga aaacagagcc tgatacttct
     3481 aacgttactg atagtacgaa aggtattgta actgttaaga tcggaaatcg aactttcgac
     3541 gtggatgtag agtttgctgt aaaagcttct caagctatgg aaaatgatgc aacatacgta
     3601 cctataacaa caactccaga aacgacagtt caaagtggaa aacctacatt tgataaacca
     3661 gatgttcctt tagctaaaga tgccttttca atttcagatg tctataataa gtacttcggt
     3721 aatgcaagtg ttgatgcaaa cactggtatt gttacattca ctccagctaa aggtgtagga
     3781 gaatcggagc cgattactgg aacaattcct attaaaattg tttaccaaga tggttctgta
     3841 ggcacgaccg atttagcagt aactgtaagt aaagatattt atgaaaatcc aggagaaaac
     3901 attcctgcag gctaccacaa agtaaccttc accgcagggg aaggaacaag tattgaaagt
     3961 ggaacaacag tctttgcagt gaaagacggc gtaagctt
//
LOCUS       DANKAZ010000156         3968 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000156 DANKAZ010000000
VERSION     DANKAZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            116..1006
                     /locus_tag="PWY67_002699"
     CDS             116..1006
                     /locus_tag="PWY67_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL2058900.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1079..1903)
                     /gene="nadE"
                     /locus_tag="PWY67_002700"
     CDS             complement(1079..1903)
                     /gene="nadE"
                     /locus_tag="PWY67_002700"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL2058901.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1915..3387)
                     /locus_tag="PWY67_002701"
     CDS             complement(1915..3387)
                     /locus_tag="PWY67_002701"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL2058902.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         1179 a          801 c          688 g         1300 t
ORIGIN      
        1 ttttatcgat tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa
       61 cttttattaa caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca
      121 actacaaata aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat
      181 caagatcatc cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact
      241 tggagtgagc agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat
      301 caaaacagaa aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat
      361 tagttttcca gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg
      421 tcactataca aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat
      481 cgggaaagtg gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct
      541 ctggtttacc agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac
      601 attggagatg ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa
      661 ttggccttca gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc
      721 tggagatttt gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa
      781 tcaatacggc ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga
      841 acccttgtca aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg
      901 aatcgaagtg aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt
      961 cggtaattac gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct
     1021 attttataaa acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt
     1081 atttccagaa atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc
     1141 accatgcttc gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat
     1201 caatttcatt atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt
     1261 caagatctgc agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca
     1321 attgtttccc ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac
     1381 caaatttcgt gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc
     1441 ctgctctttc tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc
     1501 ctttgttaaa atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa
     1561 cagctggttt gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt
     1621 ttgcatcttc ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt
     1681 tttcatcttt tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta
     1741 aagtagaatc ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt
     1801 tcaaatatgc tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg
     1861 gtttgacacc taattcattg ataatttctg tctgcaggct catttattct cctcctatgc
     1921 ttctgtatga agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt
     1981 ccagcattca gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc
     2041 ttcccatagt gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa
     2101 ttcgtaaact ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc
     2161 tcgaacagtt ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc
     2221 tcgagggtct tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg
     2281 agtgatgcgc cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa
     2341 cttgatcgta tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc
     2401 accaagggct ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat
     2461 tttagctttt tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata
     2521 gatttttgct tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata
     2581 ggccatatct ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt
     2641 cgccacgcga atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa
     2701 cacgcagtct ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga
     2761 ttggactaac gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg
     2821 tacattgctt gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc
     2881 atccagttct tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga
     2941 tttgatacga gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc
     3001 tgtttctact aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc
     3061 aaaaactagt tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa
     3121 atactccaaa aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc
     3181 attgaacgtc agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta
     3241 tccgtggtta aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg
     3301 acctaattcc cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa
     3361 actgtcgtct gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc
     3421 ctacccatta taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag
     3481 cacatgtttt acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc
     3541 ttattggtat ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac
     3601 gaagaaatta gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca
     3661 atcgtagccg ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa
     3721 tcaatggcaa aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa
     3781 agaaacggat ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat
     3841 ataccttgcg ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag
     3901 taagaaagca gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc
     3961 ttgttttt
//
LOCUS       DANKAZ010000157         3956 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000157 DANKAZ010000000
VERSION     DANKAZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..1481
                     /locus_tag="PWY67_002702"
     CDS             <1..1481
                     /locus_tag="PWY67_002702"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL2058903.1"
                     /translation="DRLLEQVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIAT
                     IVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSK
                     KLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTP
                     HMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTT
                     YNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFL
                     DDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLL
                     EDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEE
                     NIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDI
                     KKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1471..2688
                     /locus_tag="PWY67_002703"
     CDS             1471..2688
                     /locus_tag="PWY67_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2058904.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2701..>3956
                     /locus_tag="PWY67_002704"
     CDS             2701..>3956
                     /locus_tag="PWY67_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL2058905.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1366 a          677 c          826 g         1087 t
ORIGIN      
        1 ccgatcgatt gttggaacaa gtggttcttt tagcagatga atccttagag gaatatgaca
       61 cagtaagcaa acaaacaatg ttgtatcgtg aattactttc agatgaagaa tcaaaagaag
      121 atctgattgc cacaatcgtt gatattttgg gatatgcaat tgcaccagag tatcttttca
      181 acgtcttagc ggatcaagca aaacaagcaa cgttccaatt gaacgatcta aacaaagcct
      241 ttgtgcaatt ggcatctacc tacaatcaat ttaatgggtt atttgatgat gtggatctcc
      301 aatcaaaaaa actaggaaca gatgagcaac aacgaaatgt gacgattacc gaagtcatca
      361 agaaattgaa tgatgtagat gttttagaac acgatgggga cgtgatcggg gatgcgtatg
      421 agtttctgat cagtcaattt gcttcagaag cagggaagaa agccggagaa ttttacacac
      481 ctcatatggt ttctgatatg atggcgcaaa tcgtaacgct cgatcaaaag gaacgacgat
      541 tctttagtgt ttttgatcca acgatgggtt caggttcatt gatgttaaac gtacgtaact
      601 acttaacaca cccagataac gttaaatatc atgggcaaga actaaataca acgacataca
      661 atctagcaaa aatgaacttg attttacatg gtgtcgacgc cgaagaaatg aatctccgca
      721 atggggatac gttgaataag gactggccaa cagatgaacc ttacacattt gatgcagtag
      781 taatgaaccc accttattcg gctaattggt cagcagatac gactttctta gatgattctc
      841 ggtttaatcg ttatgggaaa ttagcgccaa aatccaaagc agattttgcg tttcttcttc
      901 atgggttcta tcatttgaag gaaacaggta ccatggccat cgtcttacca cacggagttt
      961 tattccgtgg cgctgcagaa ggggtcatcc gtcaaaaatt actagaagat ggcagtattt
     1021 atgctgtgat cggtatgcct gcgaacttgt tctttgggac atcgattcca acgacggtga
     1081 ttgtgttgaa gaagaatcgt caaactcgag atgtattatt cattgatgct agtcgtgaat
     1141 ttgttaaagg gaaaaatcaa aataaactat cggaagaaaa cattcaaaaa atcctcgaaa
     1201 cctacgctga aagaaaagat gtcgagaaat acgctcattt ggcaaccttt gatgaaatca
     1261 aagaaaatga ctataactta aatatcccac gctatgtaga tactttcgaa gaagaagaac
     1321 caattgatat ggttcatgtc ggaaatgaca tcaagaagat acgacaagaa cagcaagtgc
     1381 tagaaaaaaa acttttagaa gctatctctt ccctgcaaac aacacctgaa aatgaagcgt
     1441 ggttacaagg tgcgctggag gtgttcaaac atgagcaatg atacacaacc agaaatccgt
     1501 ttcccaggat ttacagatga ttgggaacag aggaagttgg gggacttcgt tgtagattat
     1561 gttgaaaaga caagtgtaca aaatcaattt cctatgctaa cttcatctca acaaaaaggg
     1621 atagttttgc aagaagacta ttttgcaaat cggcaagtaa ctacagaaaa taatattggt
     1681 tactttgtac tacctagagg atatttcacg ttccgtagtc gaagtgataa tgatgtgttt
     1741 gtgtttaacc gtaatgacat tattgataga gggattataa gctattttta tcctgtattt
     1801 actttaaaat ctgctgattc cgacttcttc ttaagacgta ttaacaatgg aatccaacga
     1861 caattatcta ttcaagcaga gggaacaggt caacatgttc tgtctctaaa aaagtttaaa
     1921 aatatagtag caatgtttcc tagtgaggaa gaacaacaaa aaatcggcac cttcttcaaa
     1981 caacttgacg acacgatcgc tcttcatcag cgtaagttag atttgctgaa ggaaacgaaa
     2041 aaaggattct tacaaaaaat gttccctgaa aacggagcaa aagtgccaga aatccggttt
     2101 cctggtttta cggaagattg ggaagagcgt aagttgggag aattaggtaa aactcaatcg
     2161 ggaattggat ttcctgatgc cgagcaaggt ggttctgaag gtactccatt ttttaaagtt
     2221 tccgacatga ataacattgg taatgagtat gaaatgagaa atgcaaatca ttatgtatct
     2281 aatgagcaaa ttgagcgtaa aaaatggaag ccaataaaag atgtgccagc gattatattt
     2341 gctaaagttg gcgcagcaat aatgcttaat agaaaacgtt tggtcacaag ccctttctta
     2401 atagataaca ataccatggc ttatttattt aataatactt gggatatcta ttttggtaaa
     2461 atattgtttg aaactataaa tttgccaaga tactcccaag taggggcatt gccttcttat
     2521 aacagttctg atattgagaa tatcagtgtt aaggttcctg taaaggatga acaacaaaaa
     2581 atcggtactt tcttcaaaca gctagatgat acgatcgctc ttcatcaacg taagttagat
     2641 ttactaaaag aaacgaaaaa aagcttctta caaaagatgt ttgtctagga gggaagaaga
     2701 atgaaaatta gtcaccgtga ggaagcagaa gtcgaagaac agttgattcg tgtacttgga
     2761 gaagggcata atcagtggac gtatcgtccg gatctaaaat cagaagaaga cctttgggtc
     2821 aatttacgcc aaaaaattat ctcaaataat caagcagaac tgaatgattt tcctttgacc
     2881 gataaagaat ttgaaacgat caaaacggaa ttactgctgc gaacgaaaac cccatttgat
     2941 gctgcgaaat ggttaaaagg tgaaaatggg atggctcgga tcacgattga acgggaagat
     3001 tcgcaattgg gttctgtctc acttatcctt tattccaatc aagacattgg tggaggaatc
     3061 tctacttatg aagtggtcca tcaaattgca aaacgtggaa gtaacataga agcacgggat
     3121 cgacgttttg acgtcacctt attaatcaat ggattaccga ttgtccaaat cgagctgaaa
     3181 caagtaactg caaaagatgg tgtttatcag gcatttaacc aaatcaaaaa atatgcagaa
     3241 gaaggtatgt ttcggaacaa tatcttttcg accttacagt tgtttgtggt ttccaatgaa
     3301 caaaccactc ggtattttgc aaatgcctta ccgaaagact tgcaccctaa gtttttattt
     3361 agttggcgaa cgaaagacaa tgaaaaagta gaaaacttat atgagttttg taaacaagtg
     3421 ttaaatatcc cggatgctca tcgtttgatt gcggattata caatcgtcag tgaagatcaa
     3481 gacaataaaa ccctcatggt gttacatcct tatcaagtac atgcgattca agccttgttt
     3541 attgccgcaa ataaacatca atcagggtat gtgtggcatg cgactggttc aggaaagacg
     3601 ttgactagtt ttgtatctac taagctttta gcacgaaaat cggggatcga tcgaacgatc
     3661 atgttagtgg accgcaaaga tttagataat caaaccacca cagagtttac aaaatttgct
     3721 tcggaattta atactgggat ttcttccggg aacgccaaag cgaatagctt gatcgttgga
     3781 acgggaagtg ccaaagaatt aagtgaaacc ctgttggcag atgccaatgc aaatgttgta
     3841 atcattacaa cccgtcaaaa actagatgcg gcgttgaaat atgcaaagaa acaagaagag
     3901 aaaaaaggaa ccaatcgctt ccaaaaatta atgggccaac atatcgtgtt tgtagt
//
LOCUS       DANKAZ010000158         3874 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000158 DANKAZ010000000
VERSION     DANKAZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..1256)
                     /locus_tag="PWY67_002705"
     CDS             complement(<1..1256)
                     /locus_tag="PWY67_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL2058906.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="PWY67_002706"
     CDS             complement(1270..2487)
                     /locus_tag="PWY67_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2058907.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..>3874)
                     /locus_tag="PWY67_002707"
     CDS             complement(2477..>3874)
                     /locus_tag="PWY67_002707"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL2058908.1"
                     /translation="YRELLSDEESKEDLIATIVDILGYSISPEYLFNVLADQAKQATF
                     QLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLG
                     HDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPT
                     MGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLN
                     KDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFY
                     HLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIV
                     LKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEI
                     KENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPEN
                     EAWLQGALEVFKHEQ"
BASE COUNT         1066 a          835 c          656 g         1317 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gata
//
LOCUS       DANKAZ010000159         3872 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000159 DANKAZ010000000
VERSION     DANKAZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            158..2257
                     /locus_tag="PWY67_002708"
     CDS             158..2257
                     /locus_tag="PWY67_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2058909.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="PWY67_002709"
     CDS             2453..3316
                     /locus_tag="PWY67_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2058910.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3872
                     /locus_tag="PWY67_002710"
     CDS             3421..>3872
                     /locus_tag="PWY67_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2058911.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
BASE COUNT         1540 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa ga
//
LOCUS       DANKAZ010000160         3775 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000160 DANKAZ010000000
VERSION     DANKAZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="PWY67_002711"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="PWY67_002711"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL2058912.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="PWY67_002712"
     CDS             complement(1172..1867)
                     /locus_tag="PWY67_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL2058913.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="PWY67_002713"
     CDS             complement(1880..2596)
                     /locus_tag="PWY67_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL2058914.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="PWY67_002714"
     CDS             2926..3534
                     /locus_tag="PWY67_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL2058915.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
BASE COUNT         1081 a          771 c          669 g         1254 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaac
//
LOCUS       DANKAZ010000161         3765 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000161 DANKAZ010000000
VERSION     DANKAZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            150..638
                     /gene="purE"
                     /locus_tag="PWY67_002715"
     CDS             150..638
                     /gene="purE"
                     /locus_tag="PWY67_002715"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL2058916.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            631..1755
                     /gene="purK"
                     /locus_tag="PWY67_002716"
     CDS             631..1755
                     /gene="purK"
                     /locus_tag="PWY67_002716"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL2058917.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            1768..3063
                     /gene="purB"
                     /locus_tag="PWY67_002717"
     CDS             1768..3063
                     /gene="purB"
                     /locus_tag="PWY67_002717"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL2058918.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3243..3620)
                     /locus_tag="PWY67_002718"
     CDS             complement(3243..3620)
                     /locus_tag="PWY67_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058919.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT         1203 a          664 c          845 g         1053 t
ORIGIN      
        1 atgtgaccta acttttgtca cattcctttt tttcatcttt aataaacacg aacaataaaa
       61 tgtttttctt tgttaacatt cgaaatttct ttgacatcaa aaaaagtgat tgttaaaata
      121 tgaatgtaga aaaactggga ggaaaaacaa tgacagcaaa aatctctgtg attatgggga
      181 gtacttcaga ttgggaaaca atgaaagaag catgtcaaat attggatgaa ttcggtgtgt
      241 cttatgaaaa aaaggtcgtt tctgctcatc gaaccccaga tttgatgttt gaatatgcac
      301 aaaacgctcg aaaaaaagga atcaaaatca ttattgcagg agcaggggga gccgcgcatc
      361 tgccgggtat ggtcgcatca aaaacaactt tgccggttat cggtgtaccg gtccaatcac
      421 gaacattaaa tggtttggat tcgcttcttt cgattgtcca aatgcctgga ggagtacctg
      481 tagctactac agcaatcgga aaagcgggag caattaatgc tggactactt gcgatacaga
      541 tgctttccat gtatgattta gaattggagg ctaaattggc tgaaagacgt tcaatgttgg
      601 ctaaaactgt tatagaaagt agtgatcaac ttggttaaac ctttgttgcc tggttcaacg
      661 atcgggatcg tcggtggcgg tcaattaggt cgtatgatgg cgattagtgc aaaagaaatg
      721 ggctttaaag taggtgtgct ggatcctgtg agcgattgtc cagccgcaca agttgcagat
      781 tggcacattg aaagtgtcta tgatgacacc tttgcgctga aagagcttgc cagaagaaca
      841 gatgtcatta cctatgaatt cgaaaacgtc agtgtagagg cgttaaatgc gattttaccc
      901 atgtcattta ttcctcaagg gacagacttg cttgcgatta cgcaagaccg cctgttagaa
      961 aaatcttttt tggaaacgaa taacatcgtg attgctcctt atgcaacgat cgtcagtccg
     1021 acggatatcc aagatgcgat tgacgggatc ggctatcctt gtgttttgaa aaccactcga
     1081 ggcggctatg atggcaaggg gcagtatgtt ttgaaaagtc gggcggatct agctcctgcc
     1141 atggaccttt taagagaagg aacctgtgtt ttagaagcat ggatcccgtt tgaaaaagag
     1201 atttcgatca tggtggcagg aaatggtcaa ggagatttca cgacttttcc ggtcgtggaa
     1261 aacatccacc acaacaatat tcttcatgaa acgattgctc cagccgcgat tgatcaggat
     1321 gtgatcgagg aggctgagcg tattgcccgt gtgattgcag aagcagtgag cctttcaggt
     1381 gttttagggg tagaaatgtt tttaacaaaa acaggcgggc tatatgtgaa tgaattagct
     1441 cctcgtcctc acaattctgg acattattct attgaagctt gttctatgag tcagttcgat
     1501 gcccatattc gaggtatttg tggttggcca ttaggagaaa aagtacgttt gataagcaat
     1561 gcagtaatgg tcaatgtttt gggagaacaa ctgctggaaa gctatcgctt gattgagaat
     1621 aagccagact ggcaattcca tttctatgga aaaaaacaag caaaaaaagg tcgcaagatg
     1681 ggacatatca ctatcttgac agaagacatc cagcaaacgc tggaagaaat caaacaaacg
     1741 aatatttggg attagaggag aaaaaacatg ttagaacgtt atacacgccc tgaaatgggg
     1801 aatatctgga cggaagagaa caaataccgt gcgtggctcg aagtcgagat tttagcagat
     1861 gaagcttggg cagaacttgg tgaaatcccg caagaagatg tgaaaaaaat tcgtgagcat
     1921 gcatcatttg atatcgatcg gatcttggaa atcgaaaaat taacacgcca tgatgtcgtt
     1981 gcgtttacac gagcggtttc tgaaacattg ggagaagaaa gaaaatgggt ccattatggt
     2041 ctaacaagta cggatgtcgt agatactgct tatggctatc tcatcaagca agcaaatgat
     2101 attttacggg aagacttgaa acgttttacg gaaatcattg gcgaaaaagc aaaagaacac
     2161 aaatatacag tgatgatggg tcggacacac ggtgtccatg ctgaaccaac tacttttgga
     2221 ttgaaacttg ccacatggta ttcagaaatg aaacggaaca tcgaacgatt tgaacatgcg
     2281 gcaaaaggag tagaagcagg aaaaatctca ggagcagtgg gaacgttcgc aaatatcccg
     2341 ccatttattg aagagtatgt atgtaaacaa ttggggatcc gtccacaaga aatctctact
     2401 caagttctcc ctcgggatct acatgctgaa tatttctcag cgatggcgct tattgctaca
     2461 agtattgaac gttttgcaac agagatacgc ggattgcaaa aatccgaaac acgtgaagta
     2521 gaagaatttt ttgcgaaagg tcaaaaaggt tcttctgcta tgccgcacaa aaggaatccg
     2581 atcggttcag aaaatatggc tggtctagcc agagtggtgc gcggacacat ggtcactgcc
     2641 tatgaaaatg tcacattatg gcatgaaaga gatatttctc attcttcagc agaacgaatc
     2701 atcttgcctg ataccacgat tttattgaat tacatgttaa atcgttttgg caatatcgtt
     2761 aaaaatctaa cggtctttcc tgaaaacatg aagcggaata tgtctgcaac atttggtttg
     2821 atttatagcc agcgcgttat gctgaaattg atcgataagg gaatgacaag agaagaagct
     2881 tatgatcttg ttcagccaaa aactgcgtac gcttgggaca accaagtgca gttccgcccg
     2941 ctgttagaag aagatccaac catcacatcg gtattatcta aagaagaaat agacgatgcc
     3001 tttgattatc atcatcattt gaaaaacgtt gacgtgatat tcaaacgagt aggtttggaa
     3061 taaagaagag tgagttgcaa aattcaatta aaatagaata gcaaaaataa ccaaaaaaga
     3121 agcagatccg ttagcggttc tgcttctttt agtttattat attttcttat caataatagg
     3181 aacctgattt taaaagcggc tacctcttta ccgtgcctct ttacatacaa aaaatagagg
     3241 aattaagcgt aaatggcttg gctattataa ctaaaatgct ggttatggag atgttgcagc
     3301 aagttttcga gaaaagcgta ttccatctct tgtccattga ataagatctg gatacgataa
     3361 tgatttttga aacgcttctg ttcatgaatc tgactgattg aaagttcgcc tttgtgttct
     3421 gtctgataaa gaaaagtcct tttacggcat tcagcacagc gcttggttgc tcggcggatt
     3481 tttcggaatg tataaaaact agtatcttta ttgcataaat aaacgtcttt gatcgacagc
     3541 aaatcattat atagacagga acgttcttca gctgttaatt ctgataaaaa aagctgacct
     3601 tgttttattg catcttccat cggcaagtgc ctcctcgaac ccattatcca aatttttagt
     3661 taattctaat ataccatcat taagtataaa tgtaaacgct tgttgatggt ataagatttt
     3721 cgtttctgat aaaacacgaa cattaaaaga atctaatgcc ttaaa
//
LOCUS       DANKAZ010000162         3714 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000162 DANKAZ010000000
VERSION     DANKAZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="PWY67_002719"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="PWY67_002719"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL2058920.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="PWY67_002720"
     CDS             complement(2260..2511)
                     /locus_tag="PWY67_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL2058921.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="PWY67_002721"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="PWY67_002721"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL2058922.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="PWY67_002722"
     CDS             3461..3679
                     /locus_tag="PWY67_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058923.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1027 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaa
//
LOCUS       DANKAZ010000163         3654 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000163 DANKAZ010000000
VERSION     DANKAZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            75..470
                     /locus_tag="PWY67_002723"
                     /pseudo
     CDS             75..470
                     /locus_tag="PWY67_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(465..683)
                     /locus_tag="PWY67_002724"
     CDS             complement(465..683)
                     /locus_tag="PWY67_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058924.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="PWY67_002725"
     CDS             790..1104
                     /locus_tag="PWY67_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2058925.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="PWY67_002726"
     CDS             1208..2386
                     /locus_tag="PWY67_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2058926.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="PWY67_002727"
     CDS             2789..3082
                     /locus_tag="PWY67_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL2058927.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1253 a          532 c          714 g         1155 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatc
//
LOCUS       DANKAZ010000164         3606 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000164 DANKAZ010000000
VERSION     DANKAZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            187..1953
                     /locus_tag="PWY67_002728"
     CDS             187..1953
                     /locus_tag="PWY67_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL2058928.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1968..2405
                     /locus_tag="PWY67_002729"
     CDS             1968..2405
                     /locus_tag="PWY67_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2058929.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2418..3569
                     /locus_tag="PWY67_002730"
     CDS             2418..3569
                     /locus_tag="PWY67_002730"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL2058930.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         1213 a          637 c          773 g          983 t
ORIGIN      
        1 tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac
       61 cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc gtgccaaagt
      121 acgactattt ctctttttag taagcggata caattagagt atcaaaagga gagagggaga
      181 gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa
      241 ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt
      301 gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt ggctaccatg
      361 gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta
      421 attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga
      481 acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc
      541 aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat
      601 aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt
      661 ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc
      721 catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat
      781 gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa
      841 tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt
      901 actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt
      961 ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta
     1021 tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta
     1081 agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct
     1141 taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca
     1201 gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag
     1261 acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc
     1321 tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc
     1381 gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa
     1441 gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa
     1501 gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat
     1561 tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct
     1621 gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag
     1681 attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa
     1741 gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta
     1801 aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg
     1861 gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa
     1921 tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc
     1981 cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt
     2041 gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat gccatggtag
     2101 atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag
     2161 atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg
     2221 tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt attgcaggag
     2281 tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt gatatggaca
     2341 atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat ctagcaattg
     2401 cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc ggtcgaggat
     2461 ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat gtgaatcaca
     2521 cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca gatgaatcaa
     2581 aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag
     2641 taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg aatatcttac
     2701 cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa
     2761 catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag
     2821 aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc tttccaaatg
     2881 cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc gtccaagtgg
     2941 aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa atccaattaa
     3001 aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta tttagtgtca
     3061 atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag accattgatg
     3121 aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag gaaacaggaa
     3181 gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat attgaaaaaa
     3241 tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc gctcggacac
     3301 cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa ctacaagaac
     3361 ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat tactatgatc
     3421 ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac ttggaacaat
     3481 tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg
     3541 tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa
     3601 attttt
//
LOCUS       DANKAZ010000165         3599 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000165 DANKAZ010000000
VERSION     DANKAZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..384
                     /locus_tag="PWY67_002731"
     CDS             <1..384
                     /locus_tag="PWY67_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058931.1"
                     /translation="LDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDEELYKEFGDL
                     SGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDSNAISIK
                     DLKELLNSKRNELKQIKETNSVELE"
     gene            398..1087
                     /locus_tag="PWY67_002732"
     CDS             398..1087
                     /locus_tag="PWY67_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058932.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1212..3563
                     /locus_tag="PWY67_002733"
     CDS             1212..3563
                     /locus_tag="PWY67_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL2058933.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1218 a          475 c          753 g         1153 t
ORIGIN      
        1 ttagattctg ttaattcaaa cgctcaaata aaagatcaaa gagtagctga acataataat
       61 aaaaaaatga gtttgaccaa aaatatctat tctatgtcag atgaagaatt gtataaagaa
      121 tttggtgatt tatccgggga acagttagaa gaaagtattc caggaattag tgactcagat
      181 aaaattgttg tcttgtctgc tggtgggcaa ttaatgggag atggcaaatc cgaattagtt
      241 agttataaac aaaatcacag acagtttatt gatgttaata ctgattccaa cgctatttcg
      301 attaaggatc taaaagagct tttaaatagt aaaagaaatg aattaaagca gataaaagaa
      361 actaattcag tggaattaga gtaaaggaag attcattatg aaaaagattt ttggtgtttc
      421 tgttgggcta cttatgatgt taacatttgg aaatgcagta tatgctgctg agaaagaagt
      481 tatttcagat ccagagcagt acgcctcacg atactttgct gattttgaag aagaagataa
      541 agtacttgca ttgccagaag gtggtttttt aaaaggggaa gctaaaattg tatctatgga
      601 taatccagat ataattcaag gttcatataa ttcagaaact gatcctgctt cagtttcagt
      661 gacagaagca aaaaaaattg ttgctgaaaa attcaaaaat gatattgaaa ctactccaat
      721 tatgcctcga ggagcatttc cacccgatgt tggtactagc ggacaccttc ttgtactagc
      781 aggaggagga agctataaat cacagccatt tagtggtgcg ggatggcgtt ttggaggtat
      841 gggtgtaaaa ccagctgatg gtacaggcaa ctttttacgt tggaaatcct acattgacga
      901 tggaagagta ggaaatgtaa atcaagccaa cgcaactaaa tctggttcta ttcaaggaat
      961 ggcattgtat gctcgtgaag gattcaaata ctttgcacca aacgggcctg gttggacaat
     1021 gaatataggt atttattaca cttataatcc tgttgcaggt acgtattatg ttgttcaaaa
     1081 tatttaataa aacagcacaa agagatttct ttgtgctgtt ttattatttt tagttgttga
     1141 caaacactaa aatataaaat ataatcaaca cataaacgaa cgtatgttcg gcaagaaagc
     1201 aggggatttt tatggatgag atggttttaa gtactcagaa atggctaaac aagaaataca
     1261 gtaatgttac agggtttgat aaggttccag aaaatgggcg aacagggtgg cctacaatct
     1321 atggacttat tgaaggacta caagtagaac ttggtattac taatctagta gctaattttg
     1381 gacctaccac tgaaaaaatg tacgataatc aggttactcc taaatggggc aaaaatctac
     1441 ctaaaaatat tgtatttctt attcaaggtg ccttttggtg taaggggatt aatccaggtg
     1501 gcttcgatgg ggtatatact ccatatttag atactgctgt aaaagagttg cagactgatg
     1561 cagggtttaa tggaagcgct gtaactggag ttttggatgc gaaatgggcc aaagcgttat
     1621 ttgatatgag tgcttttgtt cttgttcccg gcggagatag taaaattaga aaaatgcagc
     1681 aatggttaaa tgtaaattat ttagaataca cgggaataat gccttgtgat ggtatttatc
     1741 aaagaaattc aaatcaagca ttaatttttg cattacaagc tgaactagga tattctccca
     1801 gtgaagcaac agggtcttat ggtaatggga caactagtaa aacttatcct gtgtctgaag
     1861 gtaactcggg aaactatgta agaataattc aatatggatt atatgttaat ggttattttg
     1921 aagacggtac attcgatggt attttcacta aatatatggg tttagaagta ctggcattcc
     1981 gaaaatttat ggtattgcct gaatatactg aaatagcaga tgttgttgta ataaaaggac
     2041 tattgtctag cgctggagat attagaagat ctgcagatgg cgcagatact tctacgcaat
     2101 taactagatc tcaaatccaa acattggttg ataatgatat aaaaatagtt ggtagatatc
     2161 ttactggtac tgttggaatt ggaaaagatg aacgaaataa gtatctaact acagaggaat
     2221 tgaataatat tttttctaaa aatttatctg tgtttcctat ctatcaagat ggaggtgcag
     2281 cattagctta ttttacttat gatcgtggct tatcagatgg taagaaggct atagatgcag
     2341 caaaaaatct aaatatacca ttaacaacag ttatttattt tgctgttgat ctagacatgt
     2401 taggggaaga aattcttgca tatgctggag aatattttaa gggggtatct gctgtaatgt
     2461 catatagcgg gtatcaaacg ggtgtttacg gaacaagaaa tgtttgttct caaataatca
     2521 ataacgggta tgcttcgttt gcatttgttt cagatatgtc gactggttat agtgggaatt
     2581 taggttttcc tatgcctaga caatggtctt ttgatcaatt tgttgaattt accataggaa
     2641 gtggcaacgg agctgttggt atcgatttaa tcgctacatc gggtagagat agcggattta
     2701 atgagttatc aaatgatact aataatgata attacattgc caaatacaat cagaaggtta
     2761 taaatcagat ggtacgtgcg tatcaatatc tatcacaagc tggtcttgat aatccttgga
     2821 accctcattt gacattttat agatatgtaa attattctgg tttgtcatgg gacatcattt
     2881 cttctcctgt aacagagcat gatagaaaaa tttatgatga atataggaat aaattaacta
     2941 atagcgaggg cctgtataat tattttatag atcctaatac tggttcaata ataggtttgc
     3001 ctcacttaat tgtgacgtta caatcacaga tgtttctagc cgacacaatc aatgatagtg
     3061 tttctgactt tgcaggttgg ttaggagatt taatgacttg ctggggtgaa gttaagaaat
     3121 taggaataca aatggagcaa ggtgtatttg cattagtagg tacggctgca ggtggaacat
     3181 ttagtctaga agatttatac caagatgttg acgcggtaaa tgtttatacc ttagcaaaaa
     3241 gaaattctga ttcaacagct ttatcggctt taaaaacgta ctattgtgat aagggtgcta
     3301 ttgatagatt taatcaattt gttaagaaca gatttggttc ttcagataaa atcgcttcag
     3361 taacaaatag tttattagaa aatgacgaaa cagttgatat cttaggtgta gggataattt
     3421 catttttcat tgataaaata gttaataatt cacttgggac agattttact tatcttgatt
     3481 ttattggaga tgaggggaaa gttgcaaatg gattttctga aaaaattatt caatttcaag
     3541 aaaatagaag taaacttaat taagaatcgt ttactattta aacagtgtcc ttttccttt
//
LOCUS       DANKAZ010000166         3584 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000166 DANKAZ010000000
VERSION     DANKAZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(152..2305)
                     /locus_tag="PWY67_002734"
     CDS             complement(152..2305)
                     /locus_tag="PWY67_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL2058934.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2599..3447)
                     /locus_tag="PWY67_002735"
     CDS             complement(2599..3447)
                     /locus_tag="PWY67_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2058935.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
BASE COUNT          975 a          771 c          603 g         1235 t
ORIGIN      
        1 caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag tttctcggta
       61 tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt cttcatactc
      121 gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata tcataatacg
      181 cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct aataagttcg
      241 gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc acgatattac
      301 tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc cctgtttttg
      361 cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga tcggtaccat
      421 ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa atattgaccg
      481 tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca ttttcgtcat
      541 tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta gcgactgttg
      601 aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat gccaaatcgg
      661 taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc ttcatataat
      721 caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc gttgtaccaa
      781 gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct tcatctaaac
      841 taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc actttgatca
      901 tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact tgtccgcttc
      961 gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca tcaatcaaaa
     1021 cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca gccttgacta
     1081 cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg atttctttgg
     1141 aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga ggattcatag
     1201 ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat gtttgataat
     1261 tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat aacattaaat
     1321 tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct tcattatcaa
     1381 gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt ttttccaaat
     1441 aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc tctgcttcat
     1501 ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc agtgaacttg
     1561 ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt tcagaagcgc
     1621 gttctgccac aacagctaac tcttgatcag taacaccaga gtttttgata tagacagtgt
     1681 ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat tgctcctcgt
     1741 ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt tcgctgttcc
     1801 ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt ttgggatcag
     1861 ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga acatcgatca
     1921 gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt gttcctctcg
     1981 tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg gagtcgtaga
     2041 tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc actcgcgcct
     2101 ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac gcaacaaata
     2161 acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac cggcgaaacg
     2221 ttttgttctt aggttcttgt gcaccttttc ttattttttc taaccaagaa gtatttaata
     2281 tttttttcat aaaatctttc atcatttcct atccttccgc ctatttacat tcatgcttca
     2341 ttgtacccaa aaacttctta ataaaaaaga aaaaatgggc ggagcaagaa attctttacc
     2401 taatttcaag ataaaatcaa ctttttgttt cacagcaatc acttaaagtt acatatccaa
     2461 ctattttttt cattaaaaaa taaacttatt tttgaaaatt atgttttctt aagtcgtgaa
     2521 acactttgat ggatcattta tttgtggaaa actacgtttt gttattctaa aaaacttata
     2581 taaaaaatag taaggttttt atttcgaatc cataaaatat tcttggattg cttctcgtgt
     2641 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
     2701 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
     2761 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
     2821 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
     2881 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
     2941 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
     3001 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
     3061 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
     3121 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
     3181 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
     3241 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
     3301 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
     3361 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
     3421 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
     3481 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
     3541 tccgcccaac agaatatctt atcccatcaa tcccacagat tcta
//
LOCUS       DANKAZ010000167         3481 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000167 DANKAZ010000000
VERSION     DANKAZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            251..508
                     /locus_tag="PWY67_002736"
     CDS             251..508
                     /locus_tag="PWY67_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL2058936.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            740..1144
                     /locus_tag="PWY67_002737"
     CDS             740..1144
                     /locus_tag="PWY67_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HDL2058937.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            1163..>3481
                     /locus_tag="PWY67_002738"
     CDS             1163..>3481
                     /locus_tag="PWY67_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2058938.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKN"
BASE COUNT         1280 a          460 c          645 g         1096 t
ORIGIN      
        1 ttggagatgg aaaataatat taattatatt gactcaggtt cagatttgct gattaggtgg
       61 caggtaataa ctaattttct tatggaatta aaaaaataca gtaatactca aacgaagttc
      121 tcttgaagtt ataaagaata caataaatat aaatttcctt tagactatat taaaatcagc
      181 aatcgaagga aaataaaaga agaaaatggt acgcaaaatc taactattag agcaaaaaca
      241 ggaagctgct ttggaagata gtcccgaatt gtttccgatg ttaaaagatc gtttaaatga
      301 attgactgaa aaacgaagac agttacaaat tcgagagaat gaaatcttag ggatctttca
      361 acaacaaggg gaaccgattc aagtcaaaga tgtccaacag atattgacta gtttggattg
      421 ctttcttatc cagagtgaga agaagcaagt taaagctttg tatcacacct ttattgagaa
      481 aatcaccttt gatcctaatc agaagtagat attgccgatc ccgatgagat tcacacctac
      541 tgtgatttga taattgaatg agtaatatca aatagccgta tccaaaccaa aggatatggc
      601 tatttttgtg ttagggatat acgtcacgta gcttgttatc aagaataagt ttctttattt
      661 ttttgctttt tctaaaactg agtatgctaa aataagaggg tatatagcaa ctttaaataa
      721 gagataggag tgtatctttt tgggtgcaga attaaatcag aagttattta gtgcggcaga
      781 taatctccgt agtaaaatgg atgcgtcaga atataaaaat tatttattgg ggctgatttt
      841 ctataaatat ttatccgacc gtttgttgga acaagtggtt cttttagcag atgaatcctt
      901 agaggaatat gacacagtaa gcaaacaaac gatgttgtat cgtgaattac tttcagatga
      961 agaatcaaaa gaagatctga ttgcaacaat cgttgatatt ttggggtatg cgatttcgcc
     1021 agagtatctt ttcaacgtct tagcggatca agcaaaacaa gcaacgttcc aattgaatga
     1081 tctaaacaaa gcctttttag caagtcatat ttatggaaaa atactgtcgg caaaattcga
     1141 atagtgtcga ggaggtgctg tgatggaaga attaaaagaa cgcattgtca attatgtcat
     1201 agagaactct gagattaaaa ataaatgggg gaacaataca gcacagaaaa tatctgaaaa
     1261 attaaatgtt agtcgtaata ttgtctctca atatttgaat gaattcgtaa aagatggaga
     1321 atttggaaaa atcaatactc gtccagtaat cttcttttct ttaccggaga aagttccaaa
     1381 tcaacaaatt tttagctctt cagaggaagc ggaaaaatgg tatgatagct cttttagggt
     1441 tggaatagaa agggttatcg gaagtcaagg aagtctgcga aatgtaattg ctcaaattaa
     1501 agcagcaatc tactatccgc caaatggctt acccatttta cttcatggcg agactggaac
     1561 tggaaaaagc tatttagcaa attatgtgta taaggatttg ttggccaata attatataga
     1621 agagaaggca tctttcattt cagttaattg tagtgaatat gccaataatc cagaactttt
     1681 cttgtccaat ttttttgggc atgtaaaagg tgcatataca ggagccgaag atggaagaaa
     1741 gggtttagca gaattagcta ataatggagt cttattttta gatgaaattc atgcattgag
     1801 caatgaatgt caggagaaac tttttcagtt tatggatacg aggaaatttc atcggatggg
     1861 tgataatgaa aactggtaca catcaagttg ctggattata tttgcaacat cagaaccacc
     1921 tgaggataaa ctattatcaa ctttgttgag aagaatacct gtaaagctaa cacttccaac
     1981 attagacagt cgttctcttt atgaaagaaa acagttaatc aagatatttt ttaaatcaga
     2041 agaagaaaga ctagcaaaga aaatactgat atctcaacaa ttattagatt ttttattaaa
     2101 ctatcgcttt agtgaaaatg ttggcaattt gaaaaatatc attcaattga cctgtgccaa
     2161 tgccttttat ttcttagatg atttggaaga actttatatt cagatgtatc atttaccaca
     2221 atgggtgaat ttcaacggca tgaaggttat gaaaagacag aatgaattaa aatatattca
     2281 aatggatgat ttggaaaaag tagacgaagt ttttaatatc aacgaactta tagagtatct
     2341 ttatcaaacg tggaagaaag acactgataa gcagcagctt tttgagaaat atgatagata
     2401 ttttagacaa aagaatgttg atatccaaaa taattcagct ttaacatatc ccacctatca
     2461 agagattcga tttagacagt atgttaagga aatagagaga gattttcaaa tagaatttga
     2521 ctattattat cttcaaataa cgtctatctt tttgaattct tggatttatc aaatccaatt
     2581 aacatcggac gataaattat attccttcat caatgatatt aatagttggt gtagtcgaga
     2641 agttcaatta gtcaatcgtt ttattcaaat aggggatatt tatttaacaa aaattcaaaa
     2701 aaatgttttg gttattttat ttaagttaat gtgtagtaaa gaatttcagc aaaaacgttt
     2761 agcactgatt ttggctcatg gagatctaac ggcaacctgt atagcaagta ctgctaatag
     2821 gatcgttgag agatatatat acgacgccat tgatatgcca ctaatgactt caagtagtga
     2881 gattgcagac aaaattaata gatacttaca agagtgtacc ggatatgaag aaattatttt
     2941 acttgttgat atgggttcac tggagaaaat ttatctaaat ttggaactag acgctcaaaa
     3001 aaaattcgga ctactaaaca atgtttcaac aagaatggct attgatgttg caaatcagat
     3061 attattagat ttaccgctta aacagattct tgaacaagta aaagaaaatc aaaatgtgga
     3121 ttatatttat gaatcatcaa atattaagaa ggatatgatt gtctgttctt gtgcaagcgg
     3181 attaggaact tcgtttaaat taaaaaagat acttgaagat agctttccaa gggatactga
     3241 tcttgtcata gaaacgtgtg actattactc tttagtgaat gaaaactatt tatcggagct
     3301 tcgtaaagag aacaatattc tttttattgt tggaacacta aatccacatc tagaagatgt
     3361 agaattcttt tcaattgaag atatgatttt aggaatgaat ataagtaaat tctccaatca
     3421 attgaataga cactttaatg ataatgaact gaaagttttg aacaataata ttctgaagaa
     3481 t
//
LOCUS       DANKAZ010000168         3430 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000168 DANKAZ010000000
VERSION     DANKAZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..1008
                     /locus_tag="PWY67_002739"
     CDS             <1..1008
                     /locus_tag="PWY67_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058939.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="PWY67_002740"
     CDS             1025..1174
                     /locus_tag="PWY67_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058940.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="PWY67_002741"
     CDS             1171..1617
                     /locus_tag="PWY67_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058941.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="PWY67_002742"
     CDS             1619..1756
                     /locus_tag="PWY67_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2058942.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..2087
                     /locus_tag="PWY67_002743"
     CDS             1794..2087
                     /locus_tag="PWY67_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058943.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2084..2308
                     /locus_tag="PWY67_002744"
     CDS             2084..2308
                     /locus_tag="PWY67_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2058944.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2305..3330
                     /locus_tag="PWY67_002745"
     CDS             2305..3330
                     /locus_tag="PWY67_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2058945.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT         1213 a          587 c          710 g          920 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa actttattta gggagagaag aaaatgaata ataaaacgtt
     2101 cgaagtacta aaatggttcg cactggtaat tattcccgca ctagctactt tcgtggggtt
     2161 agttggtaaa gcgctcaatt ggcagtacac agatatctgt gttgtcatca ttactggttt
     2221 tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga acctacaaaa tgttctcggc
     2281 tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta ctattactgc gatgagcctg
     2341 ttaacggctc tttttttatt acccattaat acgtttgctt atactattaa tgacgagtat
     2401 aatttagcgc cgaatcaagg agactccaga ttagcaattc ctaacaaaat tattttgcac
     2461 gaaactggaa tagatgcacc agcaagaaac gtagccgcca acatgaaaaa taattataac
     2521 ggaagcaatt cttatactac agatgttatt ggtgacggtg ggattgttta ccgtgtgggt
     2581 gagcaaggat atgtttcgtg gggagctggt aacgctaatc cttatgcgcc tgtacagatt
     2641 gaattacagc gcacatatga taaagcattg tttgaaaaaa actatcgagc ttatattgaa
     2701 tatacaagag atagtgcaaa aaaatatgga attccattga ctcttgatca aggaacttct
     2761 ttatttacaa aaggaatcat ttctcatttg tgggtgacaa attatgtttg ggggaaccac
     2821 acagatccat atggttactt atcgcaaatg ggagttagca aagaaaagct tgcttatgat
     2881 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
     2941 ccaacacgag ctggtgcagc taatcctacg ctgacagatg gaacaaatca cgcccacatt
     3001 gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg ctggatggca cattgctaac
     3061 tataaatacg agtatatttt cattatggac tacaatactg ggaaagaatt agctcgagta
     3121 agagctgatg gaatttatag accagatgta aaccaagctt ataatacttc tggaaatgtt
     3181 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
     3241 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
     3301 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcagtacata
     3361 actatattga caactataaa aattattcga taaaatagtg atgttatcgc atatcttcac
     3421 tatcacccat
//
LOCUS       DANKAZ010000169         3415 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000169 DANKAZ010000000
VERSION     DANKAZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..2232)
                     /locus_tag="PWY67_002746"
     CDS             complement(<1..2232)
                     /locus_tag="PWY67_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058946.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="PWY67_002747"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="PWY67_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          946 a          757 c          545 g         1167 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa ttttt
//
LOCUS       DANKAZ010000170         3409 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000170 DANKAZ010000000
VERSION     DANKAZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..158)
                     /locus_tag="PWY67_002748"
     CDS             complement(<1..158)
                     /locus_tag="PWY67_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058947.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILE"
     gene            complement(343..840)
                     /locus_tag="PWY67_002749"
     CDS             complement(343..840)
                     /locus_tag="PWY67_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL2058948.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(970..1680)
                     /gene="budA"
                     /locus_tag="PWY67_002750"
     CDS             complement(970..1680)
                     /gene="budA"
                     /locus_tag="PWY67_002750"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL2058949.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1693..3357)
                     /gene="alsS"
                     /locus_tag="PWY67_002751"
     CDS             complement(1693..3357)
                     /gene="alsS"
                     /locus_tag="PWY67_002751"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL2058950.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT          952 a          746 c          545 g         1166 t
ORIGIN      
        1 ttttctaata tttcattgcg atcgacgtca tgatattcgt ctacgcagat cacaacacaa
       61 ttttctaatt tactgattac ttctccaacc actttttgtt tcaagccaat aggttggcaa
      121 atatattttt ttcctaaaat gatagtttcg ttattcatat ttcatccatc cttaatgttt
      181 tttcattatt caaaatgata ataccattaa aatatgattg tgaaaatcaa taacacttgt
      241 gattttataa aaaaataaaa aaaccgaaaa acagaaatga ccactgtctt tccggctctt
      301 tttattcttg atttctcttc ttttttgttt caatagacga ttttagttat taataaatga
      361 aaaagcattt atattaaaat ttgctaataa aagatctaca tatttttcta gaaatgcttg
      421 atcgatttca tcataccctt tcgttactcc gcaatcgata tctaaaacac cgattaaatt
      481 accttctttt accattggca ctacgatttc tgattgtgcg gctgaatcgc aggaaatgta
      541 atttgcatgg gttttgacat catcaacgat caatgtttct tgtttttcag ctgattctcc
      601 acaaactcct tttcccatct tgatacgtgt gcaagaaact cgcccttgga acggtcctaa
      661 aatcaattca ttaccatcga ataaataata tcctgcaaag acagtctcag gcaaagtctc
      721 attcaacaat gcagatgaat ttgctaaatt tgctaaccag ttatcttctg cttctaataa
      781 tccttgttgc tgtaataata aaatttcgta tgcatctttt ttctgttttt ctgtccacat
      841 gataaaaacc gctccttttt gatctatgga gaagattata gctcagtttt tttgtaaacg
      901 ctaggtttcc tcctcaaaaa aacgagatac gtcaggttaa tcaccttgaa cgcatctcgc
      961 tgtctttttc tattctgctt gttggatttc tgctgctaaa tcagcataat caatcgtttc
     1021 gtgaacaaaa gtcggatcat caactggaaa atgctgtaca agggacgaga tgcggctaat
     1081 ctcaactttt ccttcagcca cttcaaaatc cattacatgt ccgccaaatg tatgtgtatc
     1141 atcgataaaa tgcaggtgaa atcccgctgc agcaactcct tggaataatt caggcgtaaa
     1201 gaacccgact aatgttcctt ggatattgct ttgttggaat tctggttgag actctgaaat
     1261 ttttgctagt cttctgtaag gtttttcttg cttcggcatg atacggatgt gcatattttt
     1321 gaataatcca gttatcttca ctgccaaaaa taggttttcc ccggcttctc ttactaatat
     1381 gtcttttttc aattgttctc catctgcagt ttttgagaca gaaaaactag tatctggcgt
     1441 aaaatctgta attgctgcat agggggtaag ttcacttcca tctaattggg caactgagcc
     1501 atctgatctt ccttgataag ctacgccatc taaaaagatg acttcaccat caagcccatg
     1561 caatgttccc aatcccagag ttcctttttc taatatatga gcaatcgttt ctgttccatc
     1621 catcaagccg gccatcaaag ctcctaatgt cccgtgctgg tataagattt tttcagtcat
     1681 gtcgacactt ccttaataga attgatctgg taagattgtt tctcccaatt tctcattatc
     1741 acggtaatca atcggaatat caataattac gggtccttct gtcgcaaatc cttcttctaa
     1801 agctttttct aattcctcta cacttgtagc tcggattcct ttagcgccaa aagcttctgc
     1861 atatttcaca aaatcaactg gaccaaagtc tacacctgac gcccgctgat atttcatttt
     1921 ttcttggaac tctaccatat tgtagtgtcc atcattccaa atcaaatgaa cgatattcaa
     1981 ttttttacgt accgccgttt ctaaatcctg ggcagagaat aaaaatcccc catcgccgga
     2041 aactgatacg atttgtgtat ttggacgcac caatgcagca gaaatcgccc atggcaatgc
     2101 aacaccaagc gtttgcatac cattactgaa taacaaatga cgaggttcat aacttctaaa
     2161 gtggcgtgcc atccaaatat aatgactacc tacatctacg gtaacagtca tatcatctgt
     2221 tacttttgac tgaagcgtgt taataacttc taaaggatgc aagatacctg cttctccttt
     2281 tgattgaaca atgtcccgct ccgttaattt tgcttgaaga gatgctaaat attcttttgc
     2341 atcttctgac acttgttgag gctctaatga acctgtcaat aaatcaagag tggctgagat
     2401 atcaccgatc aattctcttt caggctgcat aaaaggatcg atttcagctg gcgcttcatc
     2461 gatcacaatg atccgcgcat ctttttccgc attccagttt cttgcttcat attcgattgg
     2521 atcatagcca attgcaataa ctagatcact tcgtttcaaa agcatatctc ctggttggtt
     2581 tctaaataaa cccacacgac caaagaaatg cgcttctaat tcacgtgaga tcactccagc
     2641 tgcttggaat gtctctacaa caggcaatgc tgtcttttca actaattgcc tgatggctaa
     2701 cgtttctttt tcacttgatc ctcgcatacc tactaatagt accggtagtt ttgcttctct
     2761 aatgcgttca gctaggtaac ggatatcatc tgcagaagca gaacctaatt taggatcgct
     2821 caatggcttg atgacattac cttggacagg tgcatccact acatcttgcg ggatactgat
     2881 aaagctcgcg cctttttttg aactcttagc catacgataa gcattggcaa tcacttctga
     2941 aattgtttct ggatcttgga tttctgcact gtattttgtg attggctgga acagtgctgc
     3001 attatccatg ctttgatggg tcaacttcaa tagatcgctt cgttttactt gtccggcaat
     3061 tgctaaaact gggtcacctt ctgctgtcgc tgttaccaaa cctgtagcta agttactagc
     3121 accaggacca cttgttgcga taactactcc tggctctcct gtaatacgtc cgattgcttg
     3181 tgccataaat gcagcatttt gttcgtgacg agtcacgatt aattcaggac cttgatcctc
     3241 taattcatta aatacaccat cgatttttgc acctggtata ccgaatacat aatcaacatg
     3301 atgattaatc aaactttcta ctacagattt cgaaccttgt ttcatttttt cagccattga
     3361 acatcgtcct ttttatgctt tatttttaat tattagttag aaataatca
//
LOCUS       DANKAZ010000171         3404 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000171 DANKAZ010000000
VERSION     DANKAZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            24..305
                     /locus_tag="PWY67_002752"
     CDS             24..305
                     /locus_tag="PWY67_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058951.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            307..723
                     /locus_tag="PWY67_002753"
     CDS             307..723
                     /locus_tag="PWY67_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058952.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1375..1800
                     /locus_tag="PWY67_002754"
     CDS             1375..1800
                     /locus_tag="PWY67_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058953.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1830..1994
                     /locus_tag="PWY67_002755"
     CDS             1830..1994
                     /locus_tag="PWY67_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058954.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2952..>3404
                     /locus_tag="PWY67_002756"
     CDS             2952..>3404
                     /locus_tag="PWY67_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2058955.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLL"
BASE COUNT         1182 a          581 c          569 g         1072 t
ORIGIN      
        1 actgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
       61 ttacaagaat agataaacgt tgtcaggaac taacaaccaa aacgggcaaa aaatggagtc
      121 gaaatcaata cctaaaaatt ttaatcgaaa acgattttga ccacgaactt atgaattaca
      181 aaaaagatca attcgattta ttattcgaaa aatttattgc cattcaagca gaaaatacga
      241 acatactaaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
      301 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
      361 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgc
      421 tttttatgta tgagttatga acctgctacc aatacaacca caaccagtaa ccaagcaaaa
      481 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
      541 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtata tcaagaagtg
      601 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
      661 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
      721 taaaaaaaga aaccgaaaaa taaaaaaatt cctcgatttc tttttaggga tcggtcttcc
      781 agcaagattt gcaagttggt accaacttgc gaaaaaaaaa agaaaaatcc gcttctgcga
      841 attcttcttt tttcattttg gggcgctgcc ctaatcccta agtcccggtt attcaatagc
      901 atggaaactc aaagaataag acgttccctg gaataaaaaa aaggctgctc gttcgagcag
      961 tccatacata ggaaaatgaa aaaacgacgt cccttactga gaaacgctat taaactttta
     1021 acaaatcctc taaaaaataa caagtcttta aaaagtgacc ggtaaaaatg cacaaagaac
     1081 taaaaaagag aaattgcatc aaatcaagca ttagaagcta gtaactagct agaccaattg
     1141 aaaatcacaa agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtatacat
     1201 tatgtcaaca agaatttaat ataacatatt tttctagttt gttataacaa tgccactcta
     1261 acaaaaatgt aagcattatc cttattaaaa gctccttgac aaggactata ccttttattt
     1321 tatgattttt acatagaaaa cgttttctct attttttgtt tagaggtgat ttttatgatt
     1381 tgcaagaaca agaaaaaaat tattgctatt attgctagtt tctctttgat ttttagtact
     1441 gcatgtactg tcttattaac aaataatgct aatgttgaag ctgcaagctc ttcttacaaa
     1501 cataatcatg ctggttatag atgttacaaa atgagttatt atgtaagtcc ttctaaatgc
     1561 aaacaattgg ctcgccaaac taataaactt aataccgtct cagctattgc aaactttatt
     1621 ggactatctg gattaacacc ggggattata tcattagttt tcggatcagc agttagtgct
     1681 aataatgtat ttgttacagc cgctagaaga ggaaaaggcg ttcaattaaa ttatattgcc
     1741 cattttagta ataccactac ctataattat agttctaacc aaaattatgt tataaaataa
     1801 ttaaaacttt aaactaggag gaatgcttta tgaaattaac aaaaattgct gtaattttag
     1861 ctgttattgc tggcattatt ttatattttg tattgcctgc taatcaattc ttcctttact
     1921 ttggaatacc tgcgatttta atagtaaatg gcgtaagaat ctttgaacaa cgaaaaaaat
     1981 gtagctctac ctaatcgtta caaaacaaca atttcacctt cggtgaaata agcgaaactt
     2041 tacaaaaagt ggctagcaaa tgctagcctt ttttgtattg tagagcgtga attttgaggg
     2101 ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcaccaa atacctccgt
     2161 atttggctag caatccttct aaaaaataca cctatcgatc agagtcaaaa caaatcaatt
     2221 ttcgagtgag cgaatccgaa tttgcaaagc tgaagctttg caaatgagtg tgccggcttc
     2281 cgtaaaaata catatggcta taatttgcgt tttaaggcgt tttaaacgtt ctggtatata
     2341 attataccaa aacaacttta aaacgcctta gatctaattt tagaggtctt atagaacatt
     2401 ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgc tttgagaaca agataagggt
     2461 tttatactaa gactgtctta aaacggctta aacaaacgct cagaactttt caaaggagtt
     2521 ctttatgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagtat aatgtgagag
     2581 cgtagcgatc acacattttt agtgaccgta gggagcgagt ttcccagttt agaaaaattc
     2641 gcgaacttgt ttgaggaaaa aagcaggagt tttttaggcg ggaaattttg cctcctcttt
     2701 tataatctct ttttaaacct ctttttaaac ctctttaccg tcggctgtat gcttactctc
     2761 ccaaggtgtg cagaatcttt tgggggtctt tttgtctgtt tttaggggtc tttttgtctg
     2821 tttttagggg tctttttgtc tgtttttggg ggtctttttg tctgttttta ggggtcttta
     2881 attaggggct tgacccctat tagaaagctc tttataattt tgttaagtag ttaatcttga
     2941 aggaggattt tatggcaaat gaaattgtga aataccacca tgaattaaat acgattcctt
     3001 tacgcaaatt tacgtcagta gaaatgaatt tattcttctc tatagtatct agaatgcgtg
     3061 acgtaggcga taaaaaagta caatttacat tcgaacaatt aaaagattta agcaattata
     3121 aagcaactgc aaatgttcga ttcatagatg atttagaaac aacgtatgac aaattaatgg
     3181 atttacgttt tggtagaaga agtgctgacg gtttgcaacg tgaacgattt gttttattta
     3241 atcaatttaa aatcgatgga aaagctgata ttccttttgc tgaaatacaa gtccatgaga
     3301 aagccttacc cttacttaat aatcttgaag aatgggttcg atattctctc caacaattca
     3361 atgaattaga aagcagttat tccaaaacca tgtttagatt atta
//
LOCUS       DANKAZ010000172         3401 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000172 DANKAZ010000000
VERSION     DANKAZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..767
                     /locus_tag="PWY67_002757"
     CDS             <1..767
                     /locus_tag="PWY67_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HDL2058956.1"
                     /translation="GYSLPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEK
                     KVSYDKQYIEDLYYKYMETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEF
                     VTKEEVVIISVNFEYSHIDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTS
                     YKKLLSEREVVRDNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKF
                     ALLDAMNVQMRDELSELAKKVKVTFLTDPRWVRIDK"
     gene            902..1480
                     /locus_tag="PWY67_002758"
     CDS             902..1480
                     /locus_tag="PWY67_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058957.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            1426..1944
                     /locus_tag="PWY67_002759"
     CDS             1426..1944
                     /locus_tag="PWY67_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058958.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            1952..2992
                     /locus_tag="PWY67_002760"
     CDS             1952..2992
                     /locus_tag="PWY67_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL2058959.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            3241..>3401
                     /locus_tag="PWY67_002761"
     CDS             3241..>3401
                     /locus_tag="PWY67_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2058960.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1228 a          381 c          674 g         1118 t
ORIGIN      
        1 ggggatattc attaccaaac tatatttcag cagttcataa tgctcatcct aattatgcaa
       61 gctatctgtc atataagaat actttaacaa ttgaagcaat gaatgaaata ttcgaaatga
      121 tggatgaaga aaaaaaggta tcttatgata aacaatatat agaagattta tactacaaat
      181 atatggaaac tgggaaaata caggaggaat atatggaaga actgagaaaa tttctctcag
      241 acaaaaaaat tctactgatt gcaccaggaa aaagttctgt agatgaggta gaaagaataa
      301 aagagtttgt taccaaagaa gaagtcgtaa taattagtgt aaattttgaa tattctcata
      361 tagatataga ttacgtattt ttaagtaatt taagacgttt tcatgaatta ccgaaagaaa
      421 gcagaactaa atgtattgtt acatcaaata tacaagcatc ggatgtatat ttacggacga
      481 gttataaaaa gttgttatct gaaagggaag ttgtaagaga caatgcagga ttaatggcaa
      541 ttagattttt agccgatatg aatgtggatg aaatattctt agctggattt gatggatatt
      601 cacataatga agatgagaat tatggtgagc aatccatgga aattataaca aaatttgcat
      661 tattagatgc aatgaacgtg caaatgagag atgaattgtc agaattagct aaaaaagtaa
      721 aggtgacatt tttaacggat ccacgttggg ttcgaattga taagtagtag taataatgtt
      781 tttaaaattt tgataaagag atagtgagag aataaaaatg ggtaaagtct taaataaaaa
      841 agaagaaaaa tatttaaggg acgatggggc gagatgaaac tctaattttg aacttttaag
      901 aatcatttct atattgtgta ttgttgctta ccatgctaat ttgcgtttaa attataatgc
      961 agacggaatt atagtacatg gttcattaac tgctaaccag attttcttga attatattgg
     1021 tatttggggt cagctaggag ttaattgttt tatatttatt tcggcatatt ttttaagtga
     1081 gaaaagaaac attcactttg aaaatgtttt gggaattgta ttagaatgta ttttttatag
     1141 tgtattagtt tatggaattc tttgtgcatg tggaatagaa acattttcat tgaacagctt
     1201 tgtaaaagaa atattatcat ttgctctagg tagatattgg tttattacat cctatttagc
     1261 attgtatatt ctaagtccgt atttaaatat catgcttaac aatctggaaa aacttcagtt
     1321 gcaaaaaata agtatgttat tgtttatagg tgtatgctgt tatcgtatgt tatttaaaac
     1381 atctttgttt ggggattgtg ctgttttaat tgcactatat gtcatatgtt atacactgaa
     1441 aaaaacgata ataatatttt tgagagacac agaatcatag gaacaattgt atgtataaca
     1501 gttataccac ttctcatatc cataggaata cttttcaaat ttataccaaa tgcaatggtt
     1561 aaccagatgg ttcatattgg ttctccattg atggttatag caagtatatt tttattttat
     1621 gtatttaaaa atttttcatt ttacagtaag tatataaata aacttgcgag aaccagttta
     1681 ggggtatata ttttacatga aaatcattat ttcattgatg gtatgaagtc tagaggctta
     1741 atatgggatg ggatatttgg attaggcaaa aggtttttag atagggattt ttggatttgg
     1801 tttataattg cagttgtagt tgtgtgcata atttgcatgg ctatcgatgt tgtaagagaa
     1861 aaatttgaaa gacagatttt taaaattaag agtgtaaaga acctgtgtaa acttatgaat
     1921 catgttataa atatataccc ataattaata aatgaggaga aaagaagtga aagttatatc
     1981 attcggagaa ttattattga gacttgcagc acccggttat tcaaagttat ttcaaagaga
     2041 tagtttggag actacattct gcggagggga agctaatgta gcggtatcct tagcaatatt
     2101 tggatatcaa tcagaattta taacaaagct tccggattca gatgttggat atgcggcagc
     2161 taatgctttg cgttattttg gagtaaatgt tgataatatt atatttggag agggacgcat
     2221 gggactgtat tatttagaaa aaggagcagc tcagagacca tctaaagtaa tttatgacag
     2281 aaaattttct gcaatttcgt tagctgatag tgatgaattt aagtggaagg agatcctaaa
     2341 cgatgcaaat tggtttcatt ggacagggat aaatccagca ttgtctgaga atctctttga
     2401 gacgtcctta gatgcatgtc aggctgccaa aaaaagaggt ataacaattt cttgtgattt
     2461 aaattatcga tccaagcttt ggacatcaga ggaagcaaaa gaaaagatgg aacagatact
     2521 accattggtt gatatttgta tttgtaatga agaagatgca gaaaaagtgc taggaattaa
     2581 agctgagcat acaaatatta atagtggaga attaaataaa gatggctatg tgcaggtggc
     2641 tagggagatt cacaagaagt ttggatgtag gtatgtagct acaactctgc gtagaagtta
     2701 ttcggcaagt agaaatggtt ggagtgcaat gttatacgaa gcagaaaaag ataaagcgta
     2761 tttttccaca gaatacaaca tacaaattgt tgatcgcgtt ggtggtggag acagcttttc
     2821 tgctggtata atatatgcgt tatgtgaaaa tatgtccgga caaaaggcta ttgaatttgc
     2881 gactgcatct agttgtttaa aacatactat agaaggtgat tttaatcgaa ctacaattga
     2941 ggatgtatat aatttaatga atagcggtgg aaatggtcgc gtgttgcgtt agtcaacata
     3001 taaagataaa tcacagtaat ttttatggtg attatctgtc ttagtagtga ttagaataac
     3061 gtgaaaataa attgttgagc gtttatactg ttttgaaaaa taaatttaac atattgaaaa
     3121 atacaaatat atacgtatat tataaagcgc cgattgtaaa attaagctag acaactaaaa
     3181 cagtcatttg tgctacactc aaaatagttc cgaccaaaga attataagga gtgagtgcaa
     3241 ataacctata cctatcttac aacggatgaa cttgtaataa tagagtctta tttgaaaatt
     3301 aaccaatctg ttgctaaaac tgcgcattgc ttgaatcgtt caagtcaaac gatccataaa
     3361 gtatatctgt tcttcaagca aagaaaatca gccttagaat a
//
LOCUS       DANKAZ010000173         3373 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000173 DANKAZ010000000
VERSION     DANKAZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            453..722
                     /gene="rpsN"
                     /locus_tag="PWY67_002762"
     CDS             453..722
                     /gene="rpsN"
                     /locus_tag="PWY67_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2058961.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            737..916
                     /gene="rpmF"
                     /locus_tag="PWY67_002763"
     CDS             737..916
                     /gene="rpmF"
                     /locus_tag="PWY67_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2058962.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            948..1325
                     /locus_tag="PWY67_002764"
     CDS             948..1325
                     /locus_tag="PWY67_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058963.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1346..1495
                     /gene="rpmG"
                     /locus_tag="PWY67_002765"
     CDS             1346..1495
                     /gene="rpmG"
                     /locus_tag="PWY67_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2058964.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1519..2502
                     /locus_tag="PWY67_002766"
     CDS             1519..2502
                     /locus_tag="PWY67_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL2058965.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2532..2654
                     /locus_tag="PWY67_002767"
     CDS             2532..2654
                     /locus_tag="PWY67_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2058966.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2672..>3373
                     /locus_tag="PWY67_002768"
     CDS             2672..>3373
                     /locus_tag="PWY67_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL2058967.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEE"
BASE COUNT         1271 a          492 c          666 g          944 t
ORIGIN      
        1 tattaaacag catctaattt tgaatacacg aaagaatcag tatgattgaa ttagttcact
       61 taatgtctat tcttataata taaggaataa tcaaagtttt aaaaaattgg cgataaacca
      121 gttaaacata aaagattgaa taaaaagtta tagtttcttt catattgccg ttttaagtag
      181 aggaaaagct acttaaaacg gtatttgtgt ttgattggac taataaaagt gaattcttat
      241 ttttcagagc ctaaactgca tattctttta aatgatttga ttatctctat gtaccgataa
      301 attcgttttc aaatagttat ctattcatat gagttttatt tcatgttatt tattgttgac
      361 aagagcaact cttgttggta aagtatcagt gtcaaaacgt aacgattacg atttgaggtg
      421 tctaaatcaa aataaaagga gtataaaggt atatggcaaa aaagtcgaaa attgctaaag
      481 caaagaaaca aatggcgatg attgaaaagt atgcagataa acgtcaggaa ttaaaagccg
      541 caggagatcg caccgctctc gctaaattgc caagagattc aaatccgaat cgtctgaggc
      601 ttcgtgatca gacggatggt cgtccaagag ggtatatgcg aaagttcggt atgtcccgta
      661 ttaagtttag agaacttgcc catcaagggt tgattcctgg agtaaaaaaa gcaagttggt
      721 aaaaggagga aatatcatgg cagtaccagc aagaaaaaca tctaaggcga agaaacggtt
      781 aagaagaact catcaaactg tagtaaaacc agaaatttca tttgacgaag cgaatggtga
      841 ttatagacgt agccatcatg tatctttaaa agggtactat aatggaaaga aagtaattaa
      901 aggagcttca aaatagttta atatataact tttaggaggg aatcaaaatg gaaattattt
      961 acccaccttt agttgaagaa ggattaaagt attatcttga aacgacccag caatcgctag
     1021 ataaatgcac tttttatcgt tcgatggtag agagaggaat tattactgaa acgggtttac
     1081 cgacccaaca ggcaattgaa aatggattag tcaaagatta ctatgaggac caaggcttat
     1141 catttgatga ttttttaaga atctatccaa tttttgaaga gtatgacgag gagttatttc
     1201 aatgtatcga tggttattgg gagatacctg ttgatatgaa agaaaattta gtttcacaat
     1261 tggaatctgg cgagttaact tttgaagatg cacaacaaat tcaagcatat cttgaagatc
     1321 gataagtatt gaaggaggaa gatttatgcg tcaaaatatt attttggaat gtgtcgaaac
     1381 tggagaacgt ctgtatctca ccagcaaaaa taaacggaat aaccctgaac ggttagaatt
     1441 aaaaaagtat tcgccaaaat tgcgcagacg ggcaattttt aaagaagtga aatagctaag
     1501 tggtaaaaag gtgacaaaat gggcatacca gttacaatcg tttctgggtt tttaggggct
     1561 ggaaaaacaa cgctaatcaa tcaagccttg caagtctcac cctaccccag ggaagaaatt
     1621 attataattg aaaatgagtt tggtgaagtt gggattgatc acaaattatt agttcatagc
     1681 gaggaacggg tgttacaact aaataatgga tgtatgtgtt gcagtcttcg cagtgattta
     1741 ttagcaatat tgatttcttt actggaaaca atagaagaac atcaacaacc aatttcacaa
     1801 atcatcattg aaactacagg tatagcagat ccccaaccaa tcattcaaac acttttaaca
     1861 gcgcctcaac taagaggaag agtatatatc gatagtttat tgactgttat tgatagtgac
     1921 agttttgaac ttattgaaag agaaccacaa gcccttaagc aacttgtcat ggcagatcgc
     1981 atttttgtgt ctagaaaaca aaacttgaat ttatctgcac taaaaaaatt acagagagaa
     2041 atcaaaagta ttaacccttt atccgatatt cgaatgttca cacatagtga aacgattcaa
     2101 aaaaaggatt tttataattt gaaaaaattt aatcatccct tagtttttga aagtgattca
     2161 tcagaaaatc actataatgg caaatactat catcaacacc atcaagtaca tgagtttaaa
     2221 tcaattctct taacttcaga tcaagacata aaaaaagatt ttttactcca atggattgac
     2281 tggctgattt taaccaacca agggagcctg tatcgtttta aaggattgat tgctttacaa
     2341 gagcaggatt ttatagttgc catgcaagag gtaaacgaac aggttaattt tcaatacacc
     2401 actcgacaaa aagaccaaac cactattatt ctaatcggaa aaatgttgga tgaagaaagg
     2461 atcaaacaga gttttgataa attactggcg gaaagcaaat aatgataagt agtgattggg
     2521 ggtaatttct aatggagaaa acggatatat ctagtgcata tcgacgactt aaaagcccga
     2581 atattaaaac tagaaaaagg gcattaaaaa ttatcaaaga acataaacga aataaaatga
     2641 agaaattagc ataattaagg aaggaaaaag aatgaagaaa atcagtgtag gacttattgg
     2701 gatagcagct ctaggattat tgggagcttg ctccagcact aatgatgcaa aagtatcgaa
     2761 tgataaagat ggaaaattag aaattgtaac aacattctat ccaatgtatg atttcaccaa
     2821 aaacatcgtc ggcgatgaag cgaacgtgga tttaatggtt ccagcgggat cagaacctca
     2881 tgactatgag ccctcagcta aggatatggc aaaagctcat gatgctgatg tttttgtata
     2941 tcataacgaa aacatggaat catgggtacc aaaagctaaa gaaagttgga agaaagctgg
     3001 gcctaatgta gtagaaggaa caaaagacat gattttattg ccaggtagtg aagaagaaga
     3061 tcatgatcac ggagaagaag atcaccatca tgaactagat cctcatacgt gggtttcacc
     3121 caaaatggca attaaggaag tttcaaatat caaggatcag ttagttaaat tgtatccgaa
     3181 aaaagcaaaa gtatttgaga caaatgctga aaagtatttg actaaactga aacggttgga
     3241 tgctgactat accactagtc ttaaagaagc gaaacaaaaa agttttgtga cgcaacacgc
     3301 agcctttgga tatttagctt tggattatgg attgaaacaa gttcctattg ctggactaag
     3361 tcctgaagaa gag
//
LOCUS       DANKAZ010000174         3283 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000174 DANKAZ010000000
VERSION     DANKAZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..1121)
                     /locus_tag="PWY67_002769"
     CDS             complement(<1..1121)
                     /locus_tag="PWY67_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2058968.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVH"
     gene            complement(1251..2627)
                     /locus_tag="PWY67_002770"
     CDS             complement(1251..2627)
                     /locus_tag="PWY67_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2058969.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2627..3283)
                     /locus_tag="PWY67_002771"
     CDS             complement(2627..3283)
                     /locus_tag="PWY67_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2058970.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
BASE COUNT          999 a          618 c          447 g         1219 t
ORIGIN      
        1 aaatggactt tgtctacagt ctgcgcattc tcattagaga acactccctt ttttgtgact
       61 tttctgaagc tagagtgaat actttcaact gcattagtag tgtacatgat ttttctaatg
      121 gcgctgccat aatcaaagag ttgttcaaca tgagaaaagt ttcgtttgca tacatctact
      181 gcaccagaat aatgagacca ccgattttga aaactgtcga aggcagcgtg tgcagcgttt
      241 aaagaagaag cactgtagaa ttttttcatg tctcgacaga cttccttata atccttactt
      301 gggaggtatc gtaacgcatt tcgaacaaga tgaacaatac agcgctgcac aatgacagaa
      361 ggaaaaattg cttttgcgcc ttctttgaga ccggaaacac cgtccatcga aatgaaaaaa
      421 acatcttcta caccacctac tttcagttca tcaaatactt gcatccagcg atttttagat
      481 tctgtttgat tgagccatag gcctaaaatc tccttgtttc ctttaagatc atagccaaga
      541 atggtgtaca cagcatattc tttagcttca tagttttcgc gtaaagtgac gtacatacaa
      601 tcaacgaata gaaatgcata acacttggct aatggccgag cttgccattc ttccaattca
      661 ggaaggacgg catccgtgat atcagaaatc atttcgtggg aaatatcaaa gccgtaaata
      721 tcttcaacgg ttgcggcgat atcgcattga ctcattcctc gtgcatacat ggaaagaacc
      781 ttaccttcga tgtcggagac atctcgtttt ctattaggaa tcaactcagg ttcaaaagaa
      841 gcctcccggt ctctgggaac atcaatgttt actttaccaa agctcgtttt aagcgtctta
      901 ttgccataac cgtttcgtcg attgtcatgt ttctttggct ctttagaatg ggcgtcatac
      961 cctaaatggt tattcaattc tccttgaagc attttttcaa ataagggacc aaacacatct
     1021 tttaaaacat cttgcatatc ctctacagat tcgggttgat aagtattcaa aatggattca
     1081 gctaacttct cagcatcagg atttcttttc tttctagcca tcgtgtaatc acctcttgat
     1141 cttattttag aaaaataaaa accgtgaagc aaccacccgc ctaggattga tggttactta
     1201 cacggtttat attacactct catttttggt gctaaaaagc accgaaaatt ttaattattg
     1261 tcaattaata attcttttgg attccttctt aatatattga atgaagaaat aatcaatgat
     1321 acaagaagta ctaaactcat aaatataact acataaatca tgaatttagg taatacattg
     1381 atatctaaac ttgataatgt cttgttaaat ccttcagctt ctgccccacc acctaattgg
     1441 ctagatgcag attgtttagc tatttgttta gctatatcac cagtaacctt attcaatata
     1501 ttattcccaa gcttgtcagc tgtatattga gctaagaaat aggaaccaac gaatgcgggg
     1561 attgatataa ataccatctc aattaggaat tgaccaaaaa tttctaattt tgatatacct
     1621 aatgatagta atactgctat ttctttcttt ctagcattca tccataagaa taataataat
     1681 gaaactacaa ctcctgcaaa tattaatgag ccaaaaaata atttatttga aattgagtaa
     1741 atacctgata tagattgttg taatgcaggg taatttgatg aacttttaat caaattatat
     1801 tctctccaat ttatatctaa tttcccaaga tcttttatta cactatcaag attcttatca
     1861 ccttttacaa agaatgttgc atcttggtat acagctgtat cttcggtatt accataaact
     1921 ttagcagctg tatgaatatc agtgattaat gtattttcgt atagttcttg tgctgcactt
     1981 actccaccgc tattatgccc atcgaataga cctttaattt ctacttctac tgtttcatct
     2041 gcacctttct cattatcagc atcaaataaa ttagatttta tttttatctt atctccaact
     2101 ttaaggttat ttttttcagc taaatcttta tgcattaaga ttttattttt atcttcattt
     2161 tctaaatgct ttccttctac taatttatat gcttctgata caaattttgt ttcttttgtt
     2221 gagtcgttaa ccccagttaa cataactgtt cttttaaaat tctttgctct ttcaggtgat
     2281 tgattcgcaa gagtctcttg agtttcaata atatcataat caactaaatc tgcaacactg
     2341 tttattcttt ttacatagga gtctatacta tctgtttgag atatcttttt aatatcttca
     2401 ccttttacat ttcccccacc tctaggtgtt cctggattta cttgtctatt tatctccata
     2461 gaaaaactat ttgttatatt agcaaatgtt tcttttgaag ctctgtccgt agcatcttta
     2521 atagataaac taatgagact taaagttgac atagataaaa taactaatat gataattaat
     2581 gattttaaac tttttctagt tacataagca aatgcatttt ttatcattat tcaacctcca
     2641 aattcatttt atttactttt ttcaactttt taccacttag ttctaaaatg atatccgcag
     2701 aatctgctac ttccttacta tgtgttacaa ctattacaca tttatttcta tctttagcta
     2761 atgtctttaa tatattaatt atttctccag cagtaacact gtctagatta ccagtaggct
     2821 catcagctag tattattgga gcatctgata ccaatgctct agcaatagct actctttgtt
     2881 gctgtccacc agataatttc ataacatttc tttttatttg ttttttatct aaacctagtt
     2941 caaacaagat actttcatct gctgatttat ttactaatct aatattttca attggtgata
     3001 aataatctat taaattatag ttttgaaata ctaaagatat attatttttt ctatgattac
     3061 tatatccttt attttgtata tcttcattct taaacaatat ttttcctgtt tgaggtttat
     3121 caagtccagc aagtaaggaa agaagtgtag attttcctgt tcctgacttt cctactatcg
     3181 cataaaactt cccaagttca aatttttgat ttactcctga caaaactttt tctttagaat
     3241 ttgcataact atatgctaca ttctttattt ctaatatatc cat
//
LOCUS       DANKAZ010000175         3246 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000175 DANKAZ010000000
VERSION     DANKAZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(1..354)
                     /locus_tag="PWY67_002772"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="PWY67_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="PWY67_002773"
     CDS             complement(614..1162)
                     /locus_tag="PWY67_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058971.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="PWY67_002774"
     CDS             complement(1163..2020)
                     /locus_tag="PWY67_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2058972.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="PWY67_002775"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="PWY67_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="PWY67_002776"
     CDS             complement(2582..2911)
                     /locus_tag="PWY67_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2058973.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            3133..>3246
                     /locus_tag="PWY67_002777"
     CDS             3133..>3246
                     /locus_tag="PWY67_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2058974.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
BASE COUNT          963 a          646 c          421 g         1216 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaacg
     3061 gttctgttgc aaagttttcc aaaaaatcta ttttagtgta aaattgagaa aaaagacaga
     3121 gaggacagag taatgaatca ttttaaaggc aaacaattca aaaaagacgt cattattgtc
     3181 gctgttggtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     3241 gatcgt
//
LOCUS       DANKAZ010000176         3232 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000176 DANKAZ010000000
VERSION     DANKAZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..306)
                     /locus_tag="PWY67_002778"
     CDS             complement(<1..306)
                     /locus_tag="PWY67_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDL2058975.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     "
     gene            510..1679
                     /locus_tag="PWY67_002779"
     CDS             510..1679
                     /locus_tag="PWY67_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2058976.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1946..2860)
                     /locus_tag="PWY67_002780"
     CDS             complement(1946..2860)
                     /locus_tag="PWY67_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058977.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     KISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(2918..3232)
                     /locus_tag="PWY67_002781"
                     /pseudo
     CDS             complement(2918..3232)
                     /locus_tag="PWY67_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1157 a          536 c          472 g         1067 t
ORIGIN      
        1 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
       61 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
      121 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
      181 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
      241 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
      301 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
      361 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
      421 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
      481 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
      541 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
      601 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
      661 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
      721 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
      781 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
      841 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
      901 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
      961 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
     1021 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
     1081 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
     1141 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
     1201 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
     1261 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
     1321 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
     1381 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
     1441 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
     1501 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
     1561 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
     1621 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
     1681 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
     1741 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
     1801 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
     1861 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
     1921 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
     1981 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
     2041 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
     2101 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
     2161 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
     2221 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
     2281 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
     2341 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
     2401 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataatagta
     2461 taaaatttct tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata
     2521 ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat
     2581 ttaaaggagt attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata
     2641 ttatacccat tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta
     2701 gtataattaa acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta
     2761 tacttccaat caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat
     2821 aaggtctatt cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg
     2881 aaagaagtaa agtaactata gcagctttta ttaatttaat aattaagaag atttctattt
     2941 tctttttccg ataaaattga ataattaaag caattataat cattaccata gtcatagcta
     3001 cagaaagaac atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt
     3061 ctttttttga gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg
     3121 cgagtaattc ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg
     3181 tatatataat agcgaaaaag gagctagtaa catgtttttt ggtaatttca ta
//
LOCUS       DANKAZ010000177         3128 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000177 DANKAZ010000000
VERSION     DANKAZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            103..1347
                     /locus_tag="PWY67_002782"
                     /pseudo
     CDS             103..1347
                     /locus_tag="PWY67_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="PWY67_002783"
     CDS             1360..1992
                     /locus_tag="PWY67_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2058978.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="PWY67_002784"
     CDS             2197..2424
                     /locus_tag="PWY67_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDL2058979.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="PWY67_002785"
     CDS             2493..2759
                     /locus_tag="PWY67_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2058980.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="PWY67_002786"
     CDS             2749..3018
                     /locus_tag="PWY67_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2058981.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DANKAZ010000178         3109 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000178 DANKAZ010000000
VERSION     DANKAZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            434..991
                     /locus_tag="PWY67_002787"
                     /pseudo
     CDS             434..991
                     /locus_tag="PWY67_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1049..3088
                     /locus_tag="PWY67_002788"
     CDS             1049..3088
                     /locus_tag="PWY67_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL2058982.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
BASE COUNT         1103 a          535 c          665 g          806 t
ORIGIN      
        1 acttatttgt tatttttttc taaaaaatat taaagattgt taaatttcac tatttcattt
       61 caaattccat tgcctaatca ttaggaaaag tctatcatga actcgtaaat agaatatagt
      121 aataaccttg ttaggtgagg ctcctataca aacataggct gttgcccaga aacgtcgaaa
      181 gtcgccaatg ggtaaaacag aaactgttgg attaaggctt tttccaatag ctaagaatgt
      241 tttctttacg ttgtatagtg ctaaaactcg gacgatgaga tgatgctagg tcagaattct
      301 tgttcgaaaa ggtttctgac cttttcttta tatttttgtg actgacttat ttgatgagac
      361 aaactcgctc ctcacctgac aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag
      421 atcgattggt ggaggtcgct cggattcact tctggttgca caatacaacc agaaaacaca
      481 acaagcaaaa ctgatctcta tcatgagaga ttcatatgtc gatattccag gttacggaat
      541 gaataagata aatgcagctt actcttatgg gggagttgat ttattaaatc aaacattaaa
      601 ggaaaatttc aaatttgaag ctccctatta tgcaagtatc acatttcaag attttatcga
      661 ttgcgttaat gaactgtttc ctggtggagt caagatcgat gcagaaaaat ctctagattt
      721 agatggcgta tatataaaca aaggagagca agtgatggat ggtaatactt tactccagta
      781 tgctcgattt cgggaagaca aagaaggaga cttcgggagg atcagaaggc agcagcaagt
      841 gatcaaagct atctctcagc agctgaaaga cgtaacttca atattcaagc tacccaaagc
      901 ggttgggaaa ttacttggta gtatccaaac gaaccttcca gaaagtgtgc ttctggattg
      961 tggtatggat ttcctcaaag acatatgtta gaataaacag gtataaatag tgaaataaag
     1021 gaatgacaag caagagaagg aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg
     1081 aacaggcgct tatattgccg gcgcagtgat cttaatagca gctgcgggtg gcggttattt
     1141 ttactaccag cactatcaag aaacccaagc agtagaagct ggagaaaaga cagttgagca
     1201 atttgtccaa gctttgaaca agggagatta taacaaagct gcagaaatga catcaaaaaa
     1261 ggcggcaaat aaaagtgcat tatctgaaaa agagatctta gataaatacc agaatatata
     1321 cggtgctgct gatgtcaaag gacttcagat atcgaatcta aaagtagata aaaaagacga
     1381 ttctacttac agtttttcat ataaagcaaa gatgaatacc tcattaggtg aattgaaaga
     1441 tctttcttat aaaggaacat tagacagaaa tgatggtcaa accacgatca actggcaacc
     1501 caacttggtt tttccagaaa tggaaggaaa tgacaaagta agcctgacca cacaagaagc
     1561 agcaagaggg aatattatag atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa
     1621 acaattagga gtcgttccaa gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa
     1681 agcgattgct tcctccttcg acttaacaga agatgctatc aatcaggcga tttcacaaag
     1741 ctgggtacaa cccgattact ttgtcccatt gaaaatcatt gatggagcaa cgccagaact
     1801 tccagctgga gctaccattc aagaagtaga cggcagatat tatcctttgg gtgaagcagc
     1861 tgctcagctg attggttacg tgggagatat cacagcagaa gatattgata agaatccaga
     1921 attaagcagt aatggaaaaa tcggtcgctc aggtttggag atggcttttg ataaggatct
     1981 tcgtgggaca acaggtggga aattaagcat tacagatgca gacggtgtag agaaaaaagt
     2041 tctgatcgaa catgaagttc aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc
     2101 acaaaaaata gcttttgaca gtctaggagg aaaagctgca tcaactgttg cgacaacgcc
     2161 aaaaaccggt gatcttcttg cgcttgctag ctctccaagc tatgatccaa acaaaatgac
     2221 aaacgggatc tcacaagaag attacaaagc ttatgaagaa aatcctgaac atccattcat
     2281 cagccgattt gcgacaggtt atgctcctgg atctacgttt aaaatgatta cagcagcaat
     2341 cggtctcgac aacggcacta tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg
     2401 gcaaaaagac agttcttggg gatcttttaa aacaacgcgt gtcagtagtg atgtatcaca
     2461 agtagactta aaaactgctt tgatctattc cgataatata tatgcggcac aagaaacgtt
     2521 gaaaatgggt gagaaaaaat ttcgtacagg cttagataaa ttcatttttg gtgaagacct
     2581 tgatttgcca attagtatga atccagcaca aatttctaat gaagatagct ttaactcaga
     2641 tatcttgcta gctgatactg gatatggaca gggcgaactt ctaattactc ctatccagca
     2701 agcagcaatg tattctgttt ttaccaacaa tggcacactt gtctatccta aattgattgc
     2761 agataaagag acaaaagata agaagaatgt catcagcgaa acagcagtac aaacgatcgt
     2821 gccagatctg agagaagttg tgcaagatgt aaatggtaca gcacattctc tttctgcttt
     2881 agggattccg ttggcagcga aaactggtac agcggaaatc aaagaaaaac aggatgtaaa
     2941 ggggcaagag aacagtttct tgtttgcttt caatcttgat aaccaaggtt atatgatggt
     3001 cagcatgttg gaaaataaag aagatgatga ttcagcaacc aaacgagcat cggaactatt
     3061 gcaatacctc aaccaaaatt atcaataaaa aattgctttt taagtacag
//
LOCUS       DANKAZ010000179         3106 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000179 DANKAZ010000000
VERSION     DANKAZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(15..707)
                     /locus_tag="PWY67_002789"
     CDS             complement(15..707)
                     /locus_tag="PWY67_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2058983.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1144..1713)
                     /locus_tag="PWY67_002790"
     CDS             complement(1144..1713)
                     /locus_tag="PWY67_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2058984.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="PWY67_002791"
     CDS             complement(1787..2110)
                     /locus_tag="PWY67_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058985.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="PWY67_002792"
     CDS             complement(2264..3076)
                     /locus_tag="PWY67_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058986.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT          857 a          627 c          546 g         1076 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacata
//
LOCUS       DANKAZ010000180         3031 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000180 DANKAZ010000000
VERSION     DANKAZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(251..865)
                     /locus_tag="PWY67_002793"
     CDS             complement(251..865)
                     /locus_tag="PWY67_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2058987.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1471..1848)
                     /locus_tag="PWY67_002794"
     CDS             complement(1471..1848)
                     /locus_tag="PWY67_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058988.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2148..3029)
                     /gene="galU"
                     /locus_tag="PWY67_002795"
     CDS             complement(2148..3029)
                     /gene="galU"
                     /locus_tag="PWY67_002795"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2058989.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          918 a          636 c          467 g         1010 t
ORIGIN      
        1 ttccactact ttttattttt ctgacactac atattcttat aactcatttc tcataactgt
       61 gaaacaactt ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc
      121 atcttaaaat aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt
      181 ctttttttca aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac
      241 atgtggataa ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa
      301 cttgctgcag caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat
      361 ttggcaaagt caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa
      421 attcaaataa tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc
      481 cagtaacaat gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg
      541 ccgggtttct tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct
      601 gatcaataat tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt
      661 caatcaatat acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac
      721 ttagtgtgcg gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc
      781 aagtgccagc ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat
      841 aatagtcaaa ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata
      901 gaaacagctg cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg
      961 tggtctttgc tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca
     1021 tagcaaataa aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc
     1081 ctagattagg tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt
     1141 cagggtgtat gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga
     1201 gccttttttt attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg
     1261 caagcaagaa aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa
     1321 aaggaatggc ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt
     1381 gaaaaaagct tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt
     1441 ttatcttttt ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg
     1501 ttcgagagaa tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga
     1561 ctttcttttc atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa
     1621 tacgcaagct tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga
     1681 tgctgcatac cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat
     1741 aaatagacta ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca
     1801 cttgagtata tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca
     1861 ccttttttta taattaaaaa attcgccttt ttattctact atgaatcttc tggcttggct
     1921 ttcatttctc ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag
     1981 cataatattt tctgaatcta ttcacgaaag tctttttcta tctttcgttt tccacgactc
     2041 aactgatcta gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc
     2101 tttaataaca aatagccttt cattctcagc aggccactta tttccagtta actttctgtt
     2161 cttagctttt tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt
     2221 tgaagtccat attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt
     2281 tttcctttaa actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc
     2341 gttagctgta tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta
     2401 ggagtcagta gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt
     2461 ttttctacaa acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata
     2521 attccgtatt tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt
     2581 gtttcttcat aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca
     2641 tcccccagca tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca
     2701 tcccccagtc ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct
     2761 tcaactaaag cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata
     2821 tttgcatcaa agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct
     2881 tcgattcccg aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt
     2941 ggaagcattt cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg
     3001 gcaggaatga ctgctttttt gaccttcact t
//
LOCUS       DANKAZ010000181         2964 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000181 DANKAZ010000000
VERSION     DANKAZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..660)
                     /locus_tag="PWY67_002796"
     CDS             complement(<1..660)
                     /locus_tag="PWY67_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2058990.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSQLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     IT"
     gene            879..2249
                     /locus_tag="PWY67_002797"
     CDS             879..2249
                     /locus_tag="PWY67_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HDL2058991.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            2270..2554
                     /locus_tag="PWY67_002798"
     CDS             2270..2554
                     /locus_tag="PWY67_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058992.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
     gene            complement(2830..>2964)
                     /locus_tag="PWY67_002799"
     CDS             complement(2830..>2964)
                     /locus_tag="PWY67_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2058993.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          984 a          446 c          603 g          931 t
ORIGIN      
        1 agtaatgccc ttaagggcaa gcttttcatg gattttatgg ctaatatatc ctttgtcccc
       61 taggatattg ggaagattcg cttcttccga taaagtctct aacactttca cgtcatggac
      121 acctggattc gtgattgagt aagtaatcgg aaagccagtc ttagtaacga tcatatgaat
      181 tttaagacca taaaaataag atttttttgt tgagttatag ccaactttgg ctatttgatt
      241 caatagtttt gcttgtctat ttctaactgg tttacataat tgactaggaa aactatctat
      301 aattccgact agttcacctt tggtgagctt cttgatgaaa aagtatcgaa taatcttgat
      361 agtgtaagct aagtttgaac ttaagcgagt gaatcgactc ctactaggaa agtcaccatt
      421 aagaaataag acggaacaaa ccgctctata cgtggctctt tggctagtat agccattaat
      481 tatgccccat ataacacaag caataatcac tgtatcgcgt tgcttcagtt gatcagtatt
      541 tcttcggttt ttaatcttat ctggtacaca attgtggtat atgttagaaa cagttctcat
      601 aatttccttg aatgttataa aagtatctgt ataatgttca gtatattttg atgaacgcat
      661 atgtagtcct catttcttgg gagttttgag actactatat gcgttttttg ttgatttacc
      721 aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt attgtttata
      781 ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat tctaccggtg
      841 aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa tctaatgaag
      901 agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt gataattcta
      961 aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag tattcagcaa
     1021 agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat gtatcaagtg
     1081 atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga ctttactata
     1141 acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg gaaagtatga
     1201 tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa tttatcactg
     1261 aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg agatatttta
     1321 atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa agtgttagga
     1381 agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat gttcttgtta
     1441 ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa tctaatctat
     1501 tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa atagagaatc
     1561 gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc atgccacaga
     1621 ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac acaggattag
     1681 taaataccat tggtaattta acactcgtta caaatcctga taattcggcc gcgggaaata
     1741 aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt atgaataaag
     1801 atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt gaagctctaa
     1861 tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat gaacacgatg
     1921 ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat ttgaacgaag
     1981 acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa gatattgcat
     2041 ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt gaagaaacaa
     2101 ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat gcagcagctc
     2161 tgctattagg gggtagccgg aatggttggg actactggaa atatgataac ggaataagta
     2221 ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt tggataagaa
     2281 caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt ttattgagct
     2341 taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt cgcgcaatgg
     2401 cttactagaa tccatggttt gggcggataa cactgttcat atctggggga gtgtttctct
     2461 ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg cattggcaaa
     2521 agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg gagaaggtag
     2581 caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg acaaagtata
     2641 gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac gtctaaatag
     2701 atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa
     2761 gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca catattatta
     2821 tttgtcaaat taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg
     2881 tgtttggtaa tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat
     2941 gaatccttga ttgacttggt tgaa
//
LOCUS       DANKAZ010000182         2862 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000182 DANKAZ010000000
VERSION     DANKAZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..1350
                     /locus_tag="PWY67_002800"
     CDS             <1..1350
                     /locus_tag="PWY67_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2058994.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="PWY67_002801"
     CDS             1484..2827
                     /locus_tag="PWY67_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2058995.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1125 a          391 c          552 g          794 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta tt
//
LOCUS       DANKAZ010000183         2826 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000183 DANKAZ010000000
VERSION     DANKAZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            798..1052
                     /locus_tag="PWY67_002802"
                     /pseudo
     CDS             798..1052
                     /locus_tag="PWY67_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="PWY67_002803"
     CDS             1122..1634
                     /locus_tag="PWY67_002803"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2058996.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="PWY67_002804"
     CDS             1631..2668
                     /locus_tag="PWY67_002804"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2058997.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1022 a          496 c          564 g          744 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacac
//
LOCUS       DANKAZ010000184         2820 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000184 DANKAZ010000000
VERSION     DANKAZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..241
                     /locus_tag="PWY67_002805"
     CDS             <1..241
                     /locus_tag="PWY67_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2058998.1"
                     /translation="SFMYRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGIS
                     ITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(406..1020)
                     /locus_tag="PWY67_002806"
     CDS             complement(406..1020)
                     /locus_tag="PWY67_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2058999.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            1258..1680
                     /locus_tag="PWY67_002807"
     CDS             1258..1680
                     /locus_tag="PWY67_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059000.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1690..1893
                     /locus_tag="PWY67_002808"
     CDS             1690..1893
                     /locus_tag="PWY67_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059001.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            2104..2688
                     /locus_tag="PWY67_002809"
     CDS             2104..2688
                     /locus_tag="PWY67_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2059002.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          957 a          425 c          504 g          934 t
ORIGIN      
        1 tagctttatg tatagagatt tttatgaaga atctatagaa ttgactccaa cggaaccaaa
       61 taaatctcaa atcactttgt caaaggagct agagagaaaa gtagagtctc aagaactagc
      121 tataggaatt agtataacca atgtgtcgac atacaaagaa ttatacgaag ctaaatcaca
      181 agataataag gattttgtag aaaatataga taaggaacca acaaatacag taggggatta
      241 acgtaaataa gtgaagatta tattaacaga ctctttattt agttaagtaa aaaatggttt
      301 gttggaattt tatgctttat ctgttctgag taagacttaa ttttatagat agtaattata
      361 tgtaggaaat taaagatgag aagataataa aagcagcatg atcaactatt tgtcattgac
      421 catgctgtct ttttttattc tatacacggt ttgtctcgta acattgtagt ctttagcaat
      481 cttagcaatc gcaacaccat ttttaagatc ttcaacgatg cttttgtaaa caaggcgacg
      541 ttggggatct tttgcgtctg cactgtataa tttgggccgc cctttgtaga cgccgtttgc
      601 ttttgcgatc ttaattcctt gtgcctggcg acgtttcgat tcagtccgtt cttgttcagc
      661 gatcatggcc aaaatttgga tgatcagatc cttaatgaat ttatccagga gaggattgcc
      721 aattgcttca gccatgatcg gtaaactagt aatgatcagc ttcacatttt tcttcttcaa
      781 gtaattgact gaagcgatga tctcgtcata atttcttcca agtcgatcaa tagcttccac
      841 aataaaaata tcttgatctc ggacgtaatc taatgcttct tggaaaacag gtcgatgagt
      901 aatcgttgcg ccagattgtt tctcgatgaa aattctctct gcaccgaatt ttttcatttc
      961 ttcaatctgg cgttgttcat tttgatcgat cgatgagaca cgtatgtatg caactctcaa
     1021 aacttttacc tactttctgc aactatttaa aatacaccct aatcatacac gaggaagcca
     1081 tatgtcactt ttctttttaa atggcaatat tttttgtaaa taaatgtcaa tcaagaaaat
     1141 ttaaacgatt atgacattaa aaatatattt tatgaatttt ctagtatata acttgattta
     1201 tctgttaaga tttattaaga aaacgttttc ttagttttga tttcgaggtg atagacaatg
     1261 aaaaataagt ttattttatc tttaggtatt ttaactattg gaattagtgt aactggaact
     1321 acaattccta catttgctga tagtacaaat gaaccaacta ctgtagtaga taataaccaa
     1381 gaattaactc ctttagatag ggacggaata gccattggta acccaactaa cgacttaact
     1441 gttactgaaa attatttaat agtttctcca ttacttgctc tagcagcttc taaaacgata
     1501 cgagtgacag gaacgatgat ttttgataaa gttggttggc cttctagacc tcctcaatca
     1561 cggaattgga aagaaaaaag aaatggttat tggtactatg gaaaattgta tttacaaagt
     1621 tataaatcag ctggaaacaa ttggattgcg acttattcag gaaccttaac tagaaagtag
     1681 ggatttttta tgccagaatt tgtgaacaca ccaaattttt atcaagatag cgatattcta
     1741 agtaaaatta atttaccgaa agaagaatca gctttacaag ttcagcgaac tttacaggag
     1801 acacaaaaaa ttcaaaaatc aatttctaat ctagaagatt ctgaagaaaa aagaaaaaaa
     1861 agagaccaaa aaacatatca aaaagaaaaa taatgatttt aaaaaaagag tgtctagtcg
     1921 acattctttt tttctaattg aaagtgttca taaaatcacc catttctgga cactttcttt
     1981 ttttgcttta tttttcaaaa aaacaattca tttcatgttc ataaacccat aacaatatct
     2041 atgttcagtt aaactatatg gataaagtta tgttacaata aaattaatga aggagtgatt
     2101 tttttgaaaa taggatacgc ccgggtatct actggtttac aaaatttgga tttacaagaa
     2161 gacagcctaa ataaatttgg ttgtgaaaaa atttttactg atcatatgag tggggcaaaa
     2221 agtaatcgac caggattaga aatggccatt gattttgttc gctctggcga tacacttgtc
     2281 gtttggcgct tagatcgttt aggtagaaat atggaagatt tgatttcaat cgtcaatcga
     2341 ctgaatgagc gtggtgtgag ttttcatagt attcaagaaa atattacgat ggacaaatca
     2401 agttctacag gacagctcat gtttcatttg tttgcggcat ttgcagaatt tgagcgtaat
     2461 ttgatcctgg aacgatccgc agcaggacga gcagctgctc gtgcaagagg acggtttggt
     2521 ggtcgtcctg aaaaattgtc tgaacaagat ttggaattac tgaagacttt agtggatagc
     2581 ggtacaccta ttaagactat tgcggaacgg tggaacgtct caagaaccac catttatcgt
     2641 tatttggcaa aaatggagga gaaaaatgga aagacaaatt ctcattagtt tttgtcattt
     2701 aatgctgaaa atttttgcca tttatctaga aaagtgacaa tagtatgaga attttttgta
     2761 agtgtctact gataacttat tctcaaatgt tgtaaggatt ttattcattt gttaatatta
//
LOCUS       DANKAZ010000185         2789 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000185 DANKAZ010000000
VERSION     DANKAZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(90..545)
                     /locus_tag="PWY67_002810"
     CDS             complement(90..545)
                     /locus_tag="PWY67_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL2059003.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(683..2611)
                     /locus_tag="PWY67_002811"
     CDS             complement(683..2611)
                     /locus_tag="PWY67_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2059004.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT          891 a          537 c          505 g          856 t
ORIGIN      
        1 tttcgaacat tgtatcacct cgttgtcagt atatcacgga tcagtaaatt gggggtgtta
       61 agaatctcct aaaatagaca aaatttgttc tattttccta aagcaaatac ttgtaagata
      121 cagtaagtga taccagaagc aatgaccggt ccggcggcga ttcctcttaa gaagacaaca
      181 cctaaaatcg tgccgaaaac aagtgcaaca gtgatttgtg gatcagaagc aatcaatcct
      241 acacccttgg aagataagac agccactagc ccaccgcaaa aaacagctac ccagccaata
      301 ggggatttaa aggcttctaa tagatcacgt agaccgattt ctcctgttgc gatagggatt
      361 agaatagccg ctgtgatgat cgtcacccca atattgattc ctttgccttg aagccaaggg
      421 aaaaaggaac gggaggctgg tatgagcttc ataatcaaaa caaaagctgc cgctatcatc
      481 agactttgat ttttagctaa aattgctaca agtaaaataa ttcctaaaaa aagccaactt
      541 tccatgaaat atattcctca ctttcttcga aacaatcata acatgttttt agaaagttac
      601 aaaagtagga attcatcctt caagacctat tcataagctg tttttttcat aaaataaaca
      661 aaatcataaa aaaacacttt gcttatttga cagaagacag ccgcgctttg tatagtggac
      721 aagacttaca tttttttgaa gaatcagaaa gatcaggtaa aggtcttagt aaacgcaagc
      781 cattcagaat aaccaatatt gtgcttcctt cgtgtcctac aactccaaga ggtagattta
      841 aaaattgcag gaagttagaa gcaatcaaca agagaataac aagaatcgaa aaaataatat
      901 tttgtttgat gatccgccgc atttttaaag atagccgatg actgtagaca aatttattta
      961 agtcgctttt cattaagacg atgtctgcga tatccatagc ggcatctgtc ccttgcccca
     1021 tagcgacacc taaagaagcg gctgctaaag caggcgcatc attgactcca tcaccaacca
     1081 tcgcatttac ttggtaaagt tcattctctt ttttgactag ttgtgttttt tcttccggag
     1141 tacagcttgc atagtaatgt tcaatcccta cttgttcagc aatggccttt gctgtgccag
     1201 aatggtcgcc ggttatcata cttgtatgga tgttttggga tttaaagtag gaaattactt
     1261 gttgggtatt tgcttttgga atatctagca gacctaaaac agcaattaac tgctgatctt
     1321 tagaaagata aatcacggtt ttgccttgat tttccaaacg ttcgatcatt tccgcaatct
     1381 ctttgctgat tatcatcgtc tcaggatcat atgcgtgctt gccaactttc cattgcgcac
     1441 ctaaataggt agtttgtaat ccaaaaccag taatttcttc tattggtaag tattggattt
     1501 cttcaggtat tgtgcaatcg aaatattgca aaatcgcatg agcaagtgga tgcgtcgttt
     1561 tttgttccat agcaacaaga agttgttgtg ccaaatcctt ttcatcaaag aaaaagctgt
     1621 cagttacaac tggttttcct ttagtgagcg ttcctgtttt atcaaaggca atcgctttta
     1681 attcagaaag cttttctaaa tggataccgc ctttgattaa aatcccattt tttgcagaat
     1741 ttgaaagagc agctagagaa gctggtgtag cagatgcgac aagcgcacaa ggagaagcca
     1801 caactaataa taccattcct cgataaaagc tttcatccca actccaacct aggaaaaagt
     1861 gggggagaag gatcattaga ggaacagcaa gcaaaatcaa ttttacataa ttattttcga
     1921 ttcgttcgat aaatgaagct gtttgagttg gcgtattctg cgcttcttct actaattgga
     1981 taatcttagc aaagacagtt tcggaactcg tttttgttac tcgaatcgtg atgggatttc
     2041 cttgattcag cgttcctgca tacatctcat cgcctaaata tttttctaca ggcacagatt
     2101 caccgcttaa tgcagactca tcgatcgttg cctgtgtgct ttctaaaaag ccatctatcg
     2161 gaacagctgc tcctttagcg acaaatacca agttatccac ctttagttga gaaacatcta
     2221 cttcttgcat ttgtccattt tcttttaata attgggcttt ttgtggttga agattcatca
     2281 aagcagtaat ttctttttga cttttattag ttgtgtattc ctctaatgca ccgcttaaac
     2341 aaaagatgaa tgtcaacatt gctccttcaa agtaattacc gatcaaacag gcaccaatag
     2401 cagctaaagc catcaataaa tccacattca gtgtccgatc attccacaat tctttcaagc
     2461 cttccgatgt ttgtttatat ccaccgatga taatggcact gatataaaaa agtggggtga
     2521 tggtaagaga gaatcggtcg aagataaaac cgaagatcat aaataaaagg cagccgattg
     2581 ttgctgcaaa agcttttttc tgattagtca tcgttattag acctccttgt caaaacaagg
     2641 atagcataat aaaaatgtaa ttgataataa taacagctaa atgagaacga taatggttat
     2701 catttggtga ttaagcttga tgttgttttt attatttata atgattatta ttaaagaaaa
     2761 taaaaaacca gaacatttct caaataaga
//
LOCUS       DANKAZ010000186         2714 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000186 DANKAZ010000000
VERSION     DANKAZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..377
                     /locus_tag="PWY67_002812"
     CDS             <1..377
                     /locus_tag="PWY67_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059005.1"
                     /translation="EWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFANTP
                     EEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYIV
                     KAKVSKRFIFNPKLEIYSVVIE"
     gene            702..1319
                     /locus_tag="PWY67_002813"
     CDS             702..1319
                     /locus_tag="PWY67_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2059006.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1360..1659
                     /locus_tag="PWY67_002814"
     CDS             1360..1659
                     /locus_tag="PWY67_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDL2059007.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1693..1860
                     /locus_tag="PWY67_002815"
     CDS             1693..1860
                     /locus_tag="PWY67_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2059008.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1905..2510)
                     /locus_tag="PWY67_002816"
     CDS             complement(1905..2510)
                     /locus_tag="PWY67_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2059009.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          950 a          409 c          495 g          860 t
ORIGIN      
        1 tagaatggaa taatctgact caaaataata aaaataattc tggatgtgga attatcttta
       61 tgtttgttat agttttggga atagtagtac ttttaataac tgtcaagata gtagattact
      121 ttgcgaatac tcctgaagaa caaatacaga gaaaacttcc tgagtttgaa agagtaccac
      181 cttatgatcc aagagataat tcatttcaag taaaaaaatt aaaagagtct ccaaatgaaa
      241 tgtggtttga agttacagtt gaatcaagcg acttcaaaga aaaaaagaaa gaatacaatt
      301 atattgtaaa ggcaaaagta tcaaaaagat ttatttttaa ccctaaatta gaaatatata
      361 gtgttgtaat agaataatag tgttaaaaaa attgtaaaag ttaaagaagt caaattctga
      421 tgtgaaaaat aaaatatacc atggaagaca taaaagaaaa actggataac tagtttattt
      481 aatggcagac cgttaaggcc tgccattttt atgtatttta gcggaataaa tataatcatg
      541 cgggagtgta aacttactgt ttcaaaaagt atactttttg acatgccttg attttctttg
      601 tttatgtatg gcataataac cacataatac aatttgaaac gtttctgatt taatctgatt
      661 gttttgaaac aattttagtg aaaaaagggt agataaaaac gatgagaaaa tttttttata
      721 tccgggtcag tagttatgat caatcgatcg aacgacaact gatttccgcg aaggaattag
      781 gaatccctga agaatatata tttgtagaaa aagccagcgg caaagatttt aaacgccctg
      841 aatatcaatt aatgaaacgg atgtttcgaa aaggagattt actctatcta caatcattag
      901 atagactagg cagaaataaa caaatgattt tagaagaatg gcgagaatta actaaggaaa
      961 agcagattga tatttctgtg ttagacatgc ctctgttaga tactgctagt tacaagcaac
     1021 tagatgggtt agagacgctt atctctgact taattctgca attactcagc tatatggccg
     1081 aagacgagcg gaaacgaatt aatagccggc aaagagaagg cgtagaagct gcgaaacaaa
     1141 agggtgtaaa attcggcaga aagaaaattg ctttacctcc tgaatttcct gcgatctatt
     1201 ctgactggaa agcaggaaag ataactgcag tctatgctat ggatcaattg ggcatgaaac
     1261 gaaatacctt ttatcgacgg gtgaaagaat atgaacatac cttgcaccac ttttcttgat
     1321 agatataaca gctattagac gggaagggag agtttgaaaa tggacttaac gaatcaaagg
     1381 gtcgttttta ctggaacgct gcagcaaatg acacgaaccc aagccatcgg tttggttcac
     1441 tctttaaacg gccattgtca atcaagtgtt acttcaaaaa caaatttcct ggtttgtggt
     1501 cagtattcta agtcgttgtt tgatgattct ctctatacaa aaaaggaaca aaccgcgcgg
     1561 gacttgattg cgttaggtca tgagattacc attttatcag aagtcgaatt ttatgcgtta
     1621 attagtcaac agttgaaaga atggcaacgg tatttataat cgttagtaaa atttacaagg
     1681 aggaaaattt ttatgaagcg aaaatcttat gatcgttggg gcattgaaat aactagtttt
     1741 aatcgaaata ttgcttttca cgtggagtgt gactgtgggg aattagcaaa aagtgtttat
     1801 caaaaaaatt acttttactg tcctaaatgc catcgccgat acggggtaca tggaacatag
     1861 aaacgaaata gtgcggtaga aaagcgaatg aatgtagtat atttctacaa atcattcgct
     1921 ttctttattt tataaatagt tggtcgagag atcccgtttt cttttgcaat ctctgcaact
     1981 ggaatgcctt gctttaacat gtccaccacg gttttaaaaa ctaagcgttt ttgcggatct
     2041 gttgagttaa tagaatactc gattggtcgt cccttatatc ggccggcttt ttgagccaat
     2101 aatactcctt gccgctgacg ttcacgaatt ttctcacgct cgttttgtgc aatataactt
     2161 agcagtgata aaaacatatc gaacatagcg gtatccagga gttcatttcc agtattaaaa
     2221 tctaaaaact gtgcatctaa tatttttaac gctacttttt tctgtttcat aaaagcaact
     2281 gtatttttta tatcttcata atctcttcct aaacgatcta gtgatgtaac gatgacgcaa
     2341 tcaccttcac ggatataatt tagtaatttt tgaaactcag gacgctcagt atctttacca
     2401 ctggcttttt cttggaataa tttatcaatg ttaagtcctt ccatcattgc caattgtcga
     2461 tccaaatttt gatcttgtga agatactcga atatagccaa tttttcccat aaaaaactcc
     2521 tttttaaaag tgatataatt tacatgtaaa atttacttgt agttttactg ctatcatagc
     2581 atttttaaaa ataaatttta tgtagactta aaatttactt catggagggt ttaaatggat
     2641 gaaatcaaaa gtaacaaaaa agcatttggc atttatttag gagaacctag tacagaacaa
     2701 ttaaaccaat tttt
//
LOCUS       DANKAZ010000187         2670 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000187 DANKAZ010000000
VERSION     DANKAZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            292..876
                     /locus_tag="PWY67_002817"
     CDS             292..876
                     /locus_tag="PWY67_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2059010.1"
                     /translation="MNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDINMID
                     NESLRRLISIATQIPYIINSSVKENILMGTKNYDKKKFSKLIELLGISKFMTKLGITL
                     DDNIYDEKILLSGGEKQKISLARALIKSASVIILDEPFRNLDNETRDNLRDYILNTKK
                     EVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            869..1897
                     /locus_tag="PWY67_002818"
     CDS             869..1897
                     /locus_tag="PWY67_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL2059011.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1953..2171
                     /locus_tag="PWY67_002819"
     CDS             1953..2171
                     /locus_tag="PWY67_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059012.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            2242..>2670
                     /locus_tag="PWY67_002820"
     CDS             2242..>2670
                     /locus_tag="PWY67_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059013.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDI"
BASE COUNT         1055 a          293 c          461 g          861 t
ORIGIN      
        1 tttcttattg atgttatttt attagctaaa ggaacaataa gtattactaa ttttgtaata
       61 gtatttatgt acgctaacat ggtccctact ttatggacta ctttaattag tattatttgg
      121 aaatggcaag actattcagt gtcattaaat agatattacg aatattattc aaataacaac
      181 aaagtggctg gcacaaaaaa aataaataaa ataaattcta ttactggtca atcattaaat
      241 ttttcatata acaaaagaaa ggtattaagc aatataagtt tttcaactga aatgaataaa
      301 ctattcatga tatcaggaga aaatggaaca gggaaaacaa cactgatgaa attatttact
      361 aaacagttgc cggttagtaa taatcaatta ttcgtcaacg ggatagatat taatatgatt
      421 gataacgaat cattaagaag gttaatttca atcgcaacac aaataccata tataattaat
      481 agctccgtga aggagaatat tttaatgggg actaaaaact atgataaaaa aaaattcagt
      541 aaattaatag aactattggg aataagtaaa tttatgacga aattaggtat tactttggac
      601 gacaatattt atgatgaaaa aatattatta tctggaggag aaaaacaaaa aataagttta
      661 gctagggcgc ttattaaatc agcttcagtt attatattag atgaaccatt tagaaattta
      721 gataatgaaa ctcgcgataa tttgagggat tatatactaa atacaaaaaa agaagtttgt
      781 tatataatta taactcatga tcacattcta gataattttt tggattcaaa tgatgaaata
      841 ttatatttaa ataaggagta tgataataat gaatagcaaa aaacttcaga tgggtgaaat
      901 tgttagaggt cctagtcaaa tatctattga tgttacatat aaatgtaatt ttagatgtaa
      961 acattgttac aatgcgagtg gtggagacaa ttgttttatg gataatgagt tgactgatga
     1021 tgaactgtta gaacttacaa aagatatatg taatttaaag ccatataata tttgtttctg
     1081 tggaggagaa actttactga gaaaaaaaat agttttatct tgtgccactt tattaaaaga
     1141 ggcaggtatt cctaatattg ctattgttac taatggatca ttagtaactc aggatgttgc
     1201 aaaagaatta ttcaaagcag gtattaatcg atttcaagta agtttagatg gaaaaaatgc
     1261 tagtagccat gaagcactta gattatatcc aaacagtttt gaaaaggcaa ttaatgctat
     1321 caaattattt agagaattgt ccgaagaagt ggatgttgct ttttgtccta ctagttttaa
     1381 ttacaatcaa ttacgagaag tatatcaact agtaaatact cttaaagtga agtctttaag
     1441 aattcaacca tttatgctta gtggtagggg aatgcaaaat agggaaatag tgccaactgt
     1501 ttctcaatat agaaaactag tttctgatat taactatatt aaaagaaatg aagattatac
     1561 caagatagat tggggagatc caattgatca tatcatccga cacagaactt tattgaaaga
     1621 tttttcgaat tttgtttcta ttaggtcaga cggaggatta atggcatcac cttacttgcc
     1681 aattcaagtt ggaaatatta aatgtcacag tctttctaaa tattggcgag caggacttta
     1741 taaagttttt tctttagatt tagttcaaga tatagctact caagtttctt caatacaaga
     1801 tatgagtaaa gaattaaaaa aaggacatat taaatggctt aatgaagata tagaaattga
     1861 tattctcgat gaatacaaac atgatatttt aatttaggtt tatcattgga aagtttagat
     1921 aaacaaagta cttttagaaa ggagggaaaa aaatgatttc tgaacctcaa atgaataata
     1981 tgtatgaaag cccaactata atgggagctg gtgaagctgc gccaagagct tgttcagcaa
     2041 ttatggtgtg tgtgttatta gcatctgcag gagtaggtat ccaagcagga gcggtgtata
     2101 cttatgctgt agttgctaca gtagctgtag ctgcagccgc agtttggaaa acagtaggat
     2161 tagttggtta ggaattaata ggagactaac tgtctcctat tgattccaat gattaagcta
     2221 aggaggaatt ttaagaaaat aatgaaatac aataatttaa aaccgaaatt acttaatgag
     2281 aacatagaat atataagaga agaaaattat ggatatttaa ccatagttcc caaaaatgga
     2341 actgaattga aagataacgt tttcaatcca acagtaagat atatattggc taactgtact
     2401 ggaaaaaaca gtgtaaaaaa aatagcgaat ggtttaaaaa agaagtttcc tagtttagaa
     2461 tatgaaaaat tctatcaaga tgttgtagga actattaaat ttttcaaaca ctataatatt
     2521 attgatatag gagattatag tatggaagaa aagaaaatat ataaaattga acaaaattta
     2581 gaattagaat tactaaatga atcacattat gaagaaattt ataattttat cacagatcat
     2641 cctgaaagta tgtattttca tgaagatatt
//
LOCUS       DANKAZ010000188         2619 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000188 DANKAZ010000000
VERSION     DANKAZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(748..>2619)
                     /locus_tag="PWY67_002821"
     CDS             complement(748..>2619)
                     /locus_tag="PWY67_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL2059014.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          756 a          517 c          414 g          932 t
ORIGIN      
        1 aattagactg gtcaaaatct ggtcaaaaaa gaggacgaat acaggtagtc ctcttttttg
       61 ttattttgat attctaaatc attttttatt ggattattcc cttgttcgat atagacccaa
      121 attgatctat caataattat caaactgagt catatagaga caaaaatttc aaaaatgacc
      181 tgttaaatgg tcatcgaata tcatttaaaa tcaatacgac ggagtcagac acggagtcaa
      241 aacaagcccg ctgagagtaa aaaatataca gtcccatagg taaagtgcca caggactgta
      301 tattttttta tttgagatta cctgaaatga ttccatttaa tttttatctc attttttcct
      361 actagttagt atcaaatact ctcctatttt ctcaataaac tattttgggt tacatggtat
      421 ctgaaacagc taaaaccttg tcattatagc aaaaaatcac tttatctccg tttcatttca
      481 gatacatttc acttaagcgg attccaaaaa aacaaaagaa aaaggaaccc ctagttagcc
      541 tatgcttttt attggttcct ctcaaaacac ggtcatataa caacggggtg gggtaaatga
      601 atgaaaaagg tggaggaaac attattcaat agctgtctta aatacttcgt tataaaatgt
      661 gaacaaataa aagacatcta tccctatttt ctacacttcc acttgactac catctttgaa
      721 tcgtacaatt ttcttaactc ttctcgttta cctcaattca ttctcaagtg gaatcaactc
      781 atcatctaac attttacgcc atgctcgctt catttcttgt gggtatttaa atggcttcgc
      841 ttctggatgt ttggctttat atccttcgta atccttactg ttgataattg ctttcatatt
      901 aggaattgga tcttcacctg gtagatattg cagattagaa gcatgtaatt ctttctcaga
      961 aacaaaccac tcattggaaa agacatgaat caaatcatca ctcgcttgtg taaaatagcg
     1021 acgtttaatg acaaagatat cttcattact atctaaagtc cccacatcaa atgcatttaa
     1081 aatttgtcgg taaacttctt tgactccttc tgttttattc aatttagcca aagctttttc
     1141 aatttcttct cgtgcatctg aactattcgc agcataagtt cctagtaatt gatcaatata
     1201 agttgaatca atatcataca acttagtggt attttcacta taaaattcga tcgttgaaaa
     1261 atcttctcct tctttttctg gttcttcctc tattaatgaa cccttcacgg tctcgtacac
     1321 gccaatttgt tcttccaaaa gttgaatttg attgttcaaa gttggagatt tcaccatgtc
     1381 atcattgtaa tcgtcataag tcactaacga ttcataagca cgattcaatt cttgaaatgc
     1441 tttgacgtac tcgatacgga tttcaagtgg cgtatgttca tcaatggatt ccatggatga
     1501 aacctctttt aacttaccat atgcttgttt gaatcttttt ttggattcct gataacttgg
     1561 ataaatcaaa cctgattctt cttttgcttt tgaatacagt tttgtcgcat cctcgacatt
     1621 cttctccatt gtcgctggct tacgaaacgt cacaatcaat ccttttgtct tatcaggata
     1681 cgtacgattc gtacgtgaaa aagcttggat caatcctgca tactctagat tacgatccac
     1741 aaatagggtt tgaatcgtag gggcgtcaaa acctgttaat agacgatcca ctacaatcgc
     1801 taaatcgatt tgtttcccaa attgtttgaa ttccgctctt tttcgtgcca aacgattatt
     1861 gatgtctccg ttataacgct caatgtctgc taatgaccag gaagtatcat aatactgatt
     1921 atattcctca ataatttttt gcatttcttc ttgttgggca cttgaatctc ggctattttc
     1981 ttctaatgaa taggtaatag cgattcgtgg aaaatcagga tcctccatcg ttcgtccttc
     2041 acgaatgagc tgattgggaa actcttttgt taaccaatca ggctctttcg tcatctcttt
     2101 gatggcccga taatactttt tggccatgtc aatggaactc gtggttaaga ttgcagattt
     2161 cgttggcctg ccatttcgaa aatcaaattt tgtataggca ttatctggac ggaacatttt
     2221 atgaatgact ttttggatat gttcatctgc ttcaaaaaca gctgggtcca aataactttc
     2281 ttttttgaca ggttccattt gatcaatcat acgattgatc tgttctgatg aatactctgc
     2341 atatgtttct acttctttta atttccgata gattttattc tctagtgaag tcggctccac
     2401 ggtgttttca tgctctactt gaaatcctaa gacagagcca tcttctagcg cattcttaat
     2461 ggtataggta tgcaagacct ctccatactg atcatgggtt gtacgtgcca attgcccttt
     2521 ggcttgtttc cgattctcag gaaaaatcgg tgtacctgta aagccaaacc aagtggattt
     2581 cggaaacatt tttttgattt cttccatatt ttcagcact
//
LOCUS       DANKAZ010000189         2572 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000189 DANKAZ010000000
VERSION     DANKAZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            177..359
                     /locus_tag="PWY67_002822"
     CDS             177..359
                     /locus_tag="PWY67_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2059015.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="PWY67_002823"
     CDS             462..746
                     /locus_tag="PWY67_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059016.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="PWY67_002824"
     CDS             762..998
                     /locus_tag="PWY67_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059017.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="PWY67_002825"
     CDS             1020..1274
                     /locus_tag="PWY67_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059018.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="PWY67_002826"
     CDS             1356..1541
                     /locus_tag="PWY67_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL2059019.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="PWY67_002827"
     CDS             1608..2003
                     /locus_tag="PWY67_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL2059020.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="PWY67_002828"
     CDS             1993..2370
                     /locus_tag="PWY67_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059021.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..>2572
                     /locus_tag="PWY67_002829"
     CDS             2392..>2572
                     /locus_tag="PWY67_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059022.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSD"
BASE COUNT          902 a          433 c          458 g          779 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tt
//
LOCUS       DANKAZ010000190         2552 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000190 DANKAZ010000000
VERSION     DANKAZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..1034
                     /locus_tag="PWY67_002830"
     CDS             <1..1034
                     /locus_tag="PWY67_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HDL2059023.1"
                     /translation="LIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLIGFF
                     IANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINSTAD
                     RYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYGNTF
                     VMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGPILS
                     AKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVGKDI
                     FIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINISYIKQLCYRGRNIIS
                     MIKSREHKR"
     gene            1070..1786
                     /locus_tag="PWY67_002831"
     CDS             1070..1786
                     /locus_tag="PWY67_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2059024.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            1805..>2552
                     /locus_tag="PWY67_002832"
     CDS             1805..>2552
                     /locus_tag="PWY67_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HDL2059025.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGK"
BASE COUNT          923 a          304 c          490 g          835 t
ORIGIN      
        1 ttctaataca atttgctaaa gggttagaaa aagttaaagc tatgggcata gcaggtatta
       61 tcagcacaat tgtaacaata attgccaata ttttcttctt gcttattctg aaatggggat
      121 taattggttt tttcattgca aatattttat cacagattat ttcagctact tatctgtcag
      181 tattagtgag aatatgggaa tatatcgatt ttgaggctaa agatcctcag ctaaagagag
      241 aaatgctaaa ttactcgatt ccattaattg caactgttgt tggatggtgg attaatagta
      301 cagctgatag gtatgtggtt tcctttatag taggtgtctc ggcgaatggc ttattgtctg
      361 tggcatataa aatacctcaa ataattaata ctttgcaggg aatatttata caggcctggc
      421 aaatttctgc aattaaagaa tatggagata atgagactgc aaaattttat ggaaatacat
      481 ttgttatgat taatttgtta atgtgcataa cctgttcaag tcttatcata ttaactagac
      541 cgttttcaca tattctttat gcgaaagact tttatgaagc gtggaaatat gttccatttt
      601 tattaatagc taatgtttta aattgtgcat caggtctgtt aggacctatc ttatcagcaa
      661 aaaaagattc taaatccatg atgtggtctg caatcatagg ggcattagtt aatgtgatta
      721 tgagtgtagt ccttgtttat aagatagggg ttcaaggtgc cacaatagcc actgttatat
      781 gttcatatgt aatatatgtt gttcgtaaac gagctgtggg aaaagatatt tttataaagc
      841 attatcatat tgttgtggaa tcttggggat tattgtttgt tcaggcaatc atagaaatat
      901 attttaaaaa ttatgtaata gaaattataa ttatgattat tttaattatt ataaatatca
      961 gttatattaa gcagttgtgt tatagaggaa gaaacattat ttcaatgatt aagagtcgag
     1021 agcacaaacg gtagacgatg ttatttttat aaaaagatat aatttattaa tggtcgataa
     1081 ttatgaaagg aaaaatattc ttaaaaaaaa gagtatagga tgtcgtatga aaatagtcgc
     1141 tatagtacca atgaagttaa ataacaaaag attgccacaa aaaaacacga aagcttttac
     1201 caatggaaaa ccattgtgtt attatatact ttcaacactt ttaaaagtaa aacagattga
     1261 taaagtgtat gtctattgta gtaacccaga tattaaagaa tttataccag aaggtgtaac
     1321 atttttaaag agatctagtt ccttagatca ggatacaacg agtattacac aggtattagc
     1381 cggtttcaca aaagaagtag aggctgatgt atatgtaatg acacacacaa cagcaccatt
     1441 tattagcgaa aaatctattg aaaaaggact agatgccgtg ctgacaggag aatatgattc
     1501 tgcatttgct gcaaaaaaat tgcaagattt cttatggaaa gacggtgaac catttaatta
     1561 tgaattggac aatattccta gaacacagga tttgccgcct ttatacgaag aaactagtgg
     1621 attttatatt tataatcgca atgtgatgac cgagttgcat agaagaattg ggaaaaaacc
     1681 ttgcatagtt gaagtaggtg agattgagtc agttgatatt gatgaagccg aggattttat
     1741 gattgctgat gcgatttata atcatcttat tttacaaggt agttaagaca gggggaaggt
     1801 taggatgaac aatgtacaga ttttagattg cacacttaga gatgggggct attgtaacaa
     1861 ttgggaattt ggatttgata atacaaaaaa aattatttct ggacttatag aatcaggaat
     1921 tgagattata gaatgtgggt ttctcacaaa taaaacagta tacaataaaa atgttacaaa
     1981 atatacgacc ttagaagaat tagcacagat aattcctgaa aaaagagaag gaaaattatt
     2041 tgtagcaatg atgaactatg gcgaatataa tatagaggat cttccagatt atgatggttc
     2101 atctattgat ggaattagga ttgcgtttca taaaaaaaat tgcaatgaag cgttgaaatt
     2161 atgtcgtgag ataaagaaaa aaggctactt agtttttgtt caagctatgg tatctctcaa
     2221 ttatacagat gaagagtttt tagcagtaat aaaaaatgtg aatcagttta aaccatacgc
     2281 tttttatatt gttgatagtt ttggaatgat gaaaggaaaa gatttaactc gtctttttta
     2341 catgattgag cataatctag aaaaagatat acagataggt tttcactcac ataataacat
     2401 gcagcttgca tattctaatg cacaaaaact tataaccgta caaacaaatc gaagcctgat
     2461 tattgattct tcggtgtacg gaatgggaag aggagcaggt aatcttaaca cagaactatt
     2521 tgtagaatat ttgaatgata atgcaggaaa aa
//
LOCUS       DANKAZ010000191         2496 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000191 DANKAZ010000000
VERSION     DANKAZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.198"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..133
                     /locus_tag="PWY67_002833"
     CDS             <1..133
                     /locus_tag="PWY67_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2059026.1"
                     /translation="NWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
     gene            234..806
                     /locus_tag="PWY67_002834"
     CDS             234..806
                     /locus_tag="PWY67_002834"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2059027.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGW
                     CYLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAR
                     EVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYI
                     EGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1162..1734)
                     /locus_tag="PWY67_002835"
     CDS             complement(1162..1734)
                     /locus_tag="PWY67_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2059028.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1750..2355)
                     /locus_tag="PWY67_002836"
     CDS             complement(1750..2355)
                     /locus_tag="PWY67_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL2059029.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          775 a          515 c          402 g          804 t
ORIGIN      
        1 caattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac cggttggtta
       61 ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg ctaacgctag
      121 cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc gaggaggctc
      181 attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat aaaatttgtc
      241 aaaaaaactg ttgccattcc atcatttcaa aagagaatct cttaaatcag gacttttcta
      301 ctgaaacgat ctgtgagaaa tgggcagctg acattacgta cattcctact aagaaaaatg
      361 gttggtgtta cttatcttca atcatggatt tacacacgaa aagaattatt agttatacat
      421 tttcaaaacg aatgactgtg gattgtgtca ttcaaacatt gaataaagca aaaatacatt
      481 atcacattcc agaaggaatg attctacaca ctgacttggg cagccaatac acagcaagag
      541 aagtggaaca atggcttaaa accaacaaaa taaggcattc ttatagtaga aaaggaacac
      601 cttatgataa tgctggaatc gaatctttcc atgcctcatt gaaaaaagaa gaagtctaca
      661 cgactagcta ctcagatttt gaagaagcaa atcgagcgct atttagctac attgaaggat
      721 tttataaccg aaatcgaatc catagctcga ttcactatct aaccccacag gaatttgaag
      781 aattagcaaa agaaaaaatg gcttaaccgt ctctactaaa gacaatcaac accgtaggga
      841 tatggagata aaatcaatag agtcaataga ttatttatcg ataataacac taaaaaagcc
      901 acgttcctaa acgtgactta cgctgaacga tatgtgttgt ttggtagacg aacaaatcgt
      961 tcattataaa tatatgatgg tatcctaata tagtaagcgt ggctagcata cctttaatta
     1021 ctttaccttt tacagttacc atacacattt tttgagcaga aatcaagtaa ataaaaaaat
     1081 attatatttc acaacatgct aatagactca tatctagaac gcattaagta tttacctgtc
     1141 tggttcgctg agacttcttt tttaactttc ttcaattttc ttctttattc taaaaagcgt
     1201 actgcgactg aaaccggtta tagcttcaac ttccttatac gatttacctg aagtaagcag
     1261 ttcatatgcg tgcctcttct tcggtgtgat tgttgctttc ggacgtcctt ctctgaaatt
     1321 aggattattc tttcttgcaa acatttttcc ttcttgggta cgttccacaa tcatatcacg
     1381 ttccatttct gctacactca acaacgtccg tgtcaccatt cgccccatgg acgtattttc
     1441 aatcgttcct agattcaaaa ccttgatcgt tacgttatca tcgagtaaag gttcaatgat
     1501 ctctaatgct tctctcgtgt tacgagcaaa tcgatcaagc ttcgtaacaa ctatttcatc
     1561 tccaggcttc agaccagcca acagctgatt gaattttggc cgatcccgtt ttgtacccgt
     1621 aaatttttct gaaaaaatat tcttcttctc tacgcagttc tcaagcaact gttgaatttg
     1681 ttcttttgta ctttgagaag cggttgagac acgtgcatat ccatatttcg tcatttggtc
     1741 cacctacttt tacaaatcat ctatcgtata ctcagaatac ccttcataga aatagctgac
     1801 atcgattcct ttcatatata actcgcgatc gtcaatttta tcagtcagag cattggaaat
     1861 tagatagcgt atttccaaat cgtttatggg acttcgctcc attgcagaaa gatagtctgc
     1921 tttatcgatt aagttccaat ctacaacttt ttgcaattct tcctttaaaa tgagatccag
     1981 ccaaattcta gtacttcggc catttccttc tcgaaatggg tgagcgatat tcatctccac
     2041 atatttcttg ataatctctt caaaagaagt ttgtggcatc tgatcaatat gctgtaacga
     2101 atgttctaaa tacataactg gtgcaaatcg aaaatttcct tttgcaatat tgaccgatcg
     2161 gatttttccg gcaaaatcgt aaatatccga aaatagaaat tcatgaattt cagataaacc
     2221 tttaaatgta ccaacttcga tttcatttat ttttccacaa tcgtacaatt ctttcgcttt
     2281 ttgtttactg agtttttctt caaccttagc caactcaact tgattggtta atcccaattt
     2341 attctctaaa atcatgcgaa atctccttaa ctttatgaca taagtttatg aaacactctc
     2401 aaccccatta tatcgcgatt ttgtttatgt gtcaaaaact attaagtttc tgacccccga
     2461 tagatttttc aagtgtctct attttgcaca gtctag
//
LOCUS       DANKAZ010000192         2458 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000192 DANKAZ010000000
VERSION     DANKAZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(87..1238)
                     /locus_tag="PWY67_002837"
     CDS             complement(87..1238)
                     /locus_tag="PWY67_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2059030.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(1392..1922)
                     /locus_tag="PWY67_002838"
     CDS             complement(1392..1922)
                     /locus_tag="PWY67_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2059031.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(2021..2371)
                     /locus_tag="PWY67_002839"
     CDS             complement(2021..2371)
                     /locus_tag="PWY67_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL2059032.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT          912 a          486 c          375 g          685 t
ORIGIN      
        1 aaaaggactg tggcgttatc gtcacagtcc ttttttcaaa taaacggttt tcacgatgtc
       61 tctttcacaa cttctttttg atttactcac acttcataaa ctttttttgt aaaatatttg
      121 agaacaagtg gagaaataag tgtacttaat aaaatcacga taactaaagg tgaatagtac
      181 tgtggttcga tcaattgact ttgctgtcca atttgtaaaa tgatcaaagc catttctcca
      241 cgcgaaatca taccagcacc gaccatcaat gaactatgtt gagaaaaacc ggcaatctga
      301 gcaccaaaat aacctccaag caatttggtc aaaatcgcta caagcgtcaa tatagaaata
      361 aagagtagtt gttcactaaa gtcagaaaaa tcaacttcta atcctacact gacaaaaaat
      421 actggtataa atactgcata tcctaaagcc tctacattgt ggtatacttc ttctttaacc
      481 tttgtctgac tgacagctat cccagcaaaa aaagcaccaa tgacagagct aagcccaaca
      541 agatccgcta cgtaagacat tcctaaacaa ataactagcg atacgatgat aatagaagaa
      601 tttgcgtaca ctttttctgc aaatgaaagt aggaaaggcg caatccattt gactaataag
      661 aaaataaaaa caaaataaat tagctgttca atcaaaacaa gcggtaaaga agcattgcta
      721 gaagcttccc ccgttagaaa agataggctg aaacttagaa ctaaaacgac taaaatgtca
      781 tctaccacag aagcacccaa aattgttgat ccttcttttg tattcactac gtttagttct
      841 tttaatactt ctactgaaat actgacagaa gttgccgcaa gaataatccc aaagaaaatc
      901 gcttcatttc ccgaaacttg gaatgctttg cctgtcaacc agccaaaaac aaccgggaag
      961 atgatcccaa gcaaagcaac gaacatacca ggtcggaaat attttttcaa aagagataaa
     1021 tcactttcca gaccagctaa aaacattaat aagatcacac caatctccga aaagtcatgg
     1081 actagaatat caggtttgac ccaattcaat ccagcttggc ctagcaaaac accaacaagt
     1141 aattgtccaa tcactgctgg gatacctaat ctccgcgaga tatgtgagcc aatcgttgtt
     1201 gaaatcaata tcaggcataa aataccaata aattccattc tcatcattcc tttgtataaa
     1261 atatagcccg aacttagcat aacatacatt attgaaaaaa taggaaaata tactcacaaa
     1321 aaaaccaaca atccaaaaga tttttttgga ttgttggttt ttatacggtc tattttaata
     1381 actaaaaatg attaacgtat agtcattttt cttattctga tgaaattgat aatctcctgg
     1441 atgcccatga aaatcaaaat aaagccgaat atttggaata gaagcatcaa acttttgaat
     1501 ggattgaaca ataagacgaa tccgccaatc aacaacagaa tactactata aacactccaa
     1561 cttttagctt ttgtattcat gctgaagaac aactggaaca agctgttgat gataacagct
     1621 aatccaagaa gaatcggcag gatagaaacg attgcaggtg caaaagcata gacgatcaga
     1681 gctaagatca ggaaaaaaag accactgaac agaccgatac tccaagaacc tgttttcctt
     1741 ttaaaacggt aagcctcaaa aagattcaac aacccaagca aaacaagata ggcggtgatc
     1801 agataaccga taaaatgaaa aacagcagaa gggtttagca caatcgcaat cccagaaata
     1861 atataaacag cagctcttaa taaggcgtat ttttgaatat tttgtttaat cgtctcaaac
     1921 atataagaaa acctcctttg acttatttta acgtgaacta gtcattttac caaattaaat
     1981 gttacaaaat gagcgaagga gaagaatatc tctggaaaaa tcaacttctg aggatctttt
     2041 ccattttttt gccttttgct agctcatcga tcattttatc taaataacga atttctttca
     2101 tcaagttatc ctcgatgtct tctactcgta caccacaaac aacacctgta atcaaggaac
     2161 gtgaaggatt ggggacaggg gcttccgcaa aaaattgttc gaaagtcact tgtttttgta
     2221 gttgatattc tagttctgtt tgggaataac ccgtcagcca acaaatgatt tcatctactt
     2281 cctcttttat acgatctttt ctctccgctt ttgctacata cataggataa acgcttgcaa
     2341 aacttgtcgt aaaaatacga ttttgcgcca taatcagtca cctttctttt tttcaaatta
     2401 tttactactt atagtatata agaaaaagag aagaaaaagc gattattcaa aaaaacaa
//
LOCUS       DANKAZ010000193         2444 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000193 DANKAZ010000000
VERSION     DANKAZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            144..494
                     /locus_tag="PWY67_002840"
     CDS             144..494
                     /locus_tag="PWY67_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2059033.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="PWY67_002841"
     CDS             908..1726
                     /locus_tag="PWY67_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2059034.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="PWY67_002842"
     CDS             complement(1799..2158)
                     /locus_tag="PWY67_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2059035.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          806 a          354 c          455 g          829 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt
     2401 ccttagtcta ttatatatct atttctctta ggagtatgtt taga
//
LOCUS       DANKAZ010000194         2418 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000194 DANKAZ010000000
VERSION     DANKAZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.201"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            176..550
                     /locus_tag="PWY67_002843"
     CDS             176..550
                     /locus_tag="PWY67_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2059036.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            555..983
                     /locus_tag="PWY67_002844"
     CDS             555..983
                     /locus_tag="PWY67_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2059037.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            1074..1988
                     /locus_tag="PWY67_002845"
     CDS             1074..1988
                     /locus_tag="PWY67_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL2059038.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            2104..>2418
                     /locus_tag="PWY67_002846"
     CDS             2104..>2418
                     /locus_tag="PWY67_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2059039.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKI"
BASE COUNT          920 a          379 c          415 g          704 t
ORIGIN      
        1 gaaatgccta gtaatttaca tttcagagtt tgccgaccgt gaagtgttta ttagttatca
       61 agtcttttta gctaatattt tagcttacga aaacagtata ctaacacttt tattagttgt
      121 caacaccttt aactaatttt tttattagtg ttttcgtagc ttgtgaggtg cgaaaatgaa
      181 tttactagat agaataaagg aattagctca taaaagaggc ataagtataa ctcaattgga
      241 agaagaatta aatataccta aaaacacgat ttatcaatgg aaaaatagaa ctcctagtac
      301 taaaagacta caacttgtag cagattattt taatgtaact actgattatt tactcggtag
      361 aaaccaagtt cctgactggg caacaaaaga tgaagtagtt gaacttgata aattactaga
      421 ttcaaatgtc aatatgtctt atggcggaga aacactgacg cctgaacagg tccaacgcgt
      481 aaaagatatc cttattggaa ccttctggga tattgtgaaa gaagacaaag aaaaaggcaa
      541 aaagatgtga gcttatggag atggatacga ttaatttagt cgaggagttg aagcggaaat
      601 accagtccgc taatcctttt tatatttgtg aaaagatggg cattaaaatt caatacgttc
      661 cttttatcga gaatcccaaa ggacagtttc aagaaattag aggtcgtgca ataatcttct
      721 taaatgatga actgagagac tctgaggaaa gattctacat ttgcgctcac gaattaggtc
      781 acgctatttt tcatcgtggc ttatccagtt actatgtatc aacaagaaca tctagaagca
      841 aatctgaaag cgaagctaat tgctttgctg ctaatctcat tgtttctcta tacaaagaag
      901 acaatgatca atatcctaaa cgaattgagg aattaaagaa tctttacgga cttccagaaa
      961 gcgcttatcg ctttcttata taatgtaaaa aaagtccgtg ctgcaacacg gactaaaaac
     1021 cttatttcta agatcctaca aaaaaatcat atcatagaag tgaggaatta aaaatgaaaa
     1081 aaattgtttt tgggttgctt tttttaggct tggtattaag cggatgctcc actagtccta
     1141 attctgctag cactaaagaa aatactgttt catcaacatc tgaatatgaa acaaaaaaac
     1201 taacttcatc agacgttcat aaaataaaag ttggtgatga tttaactcaa gtttatcaga
     1261 cacttggttc accgttacgt aaatggacaa atgattttgt ttacaaagaa ttagacaatg
     1321 ctatcacacg ggatgaatta attgtatcta tttcaaagga tgcacaagga gattattata
     1381 aagagcactc tgaattaatt gaacatggaa atagtgcaaa agatataaaa aaattagaga
     1441 tgctccaata tacttattca actgattctg atgatgaaac aatgttaatt tgggttagtc
     1501 caaaaactga acaggttgta tattataatc aacgaacttt cttagataaa gatggaaaat
     1561 ccaaagattc aagctctact acaactacaa ctacaacttc gtcttcagaa ccccagacgg
     1621 catcagttgc acatagtata ggagaagcag ttccttttac ttctgaaaat ggtaactcta
     1681 ttaacgtaac tgtccagtct gtatcaaaag attacggtga tgatttctat aaaccagaag
     1741 gcctatttta cgcaaaggta gattttcttg tagaaaacac agggactaca ccttttgatg
     1801 caagttcaag ttatttagaa ttttatgatt catcagatat taaatctgat ttagctagta
     1861 gagattactt ttcggaaacc atccaaccgg gtaaatccgc acaaggaaca gcatattttg
     1921 aagtgaaaaa tgatggaaca acatttgaag tgttcttcgc agatacttca tggaaaggta
     1981 gctactaaac aaaaaaacac gccccctccc tcgccaaagt ttgtggacgt gatgaaaaat
     2041 aaacctgcta taattggctt acttatccat tcctattata gcaacaaata ggagatgaaa
     2101 acaatgtgga ttgaaaccaa aactgataaa aacggaaaaa aagtatataa atataatgag
     2161 cgatatattg atccaaaaac tagaaaaaga aaaaaagtga gcattactta taaaaataaa
     2221 tctcgagaaa ctcagaaagt ggcattgcta gagttaaata aaaaaattga tataaaatta
     2281 aacgaaaaaa cacttcagaa gcctgatcta acatttcatg agcttgtcga agaatggtta
     2341 gttatttaca aaagacaagt taaggagtct acatattatc ctacgaataa tatcttaaac
     2401 actataaaga aaaagata
//
LOCUS       DANKAZ010000195         2408 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000195 DANKAZ010000000
VERSION     DANKAZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            6..1151
                     /locus_tag="PWY67_002847"
     CDS             6..1151
                     /locus_tag="PWY67_002847"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDL2059040.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1322..1780
                     /locus_tag="PWY67_002848"
     CDS             1322..1780
                     /locus_tag="PWY67_002848"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL2059041.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1939..>2408
                     /locus_tag="PWY67_002849"
     CDS             1939..>2408
                     /locus_tag="PWY67_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL2059042.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          456 g          698 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgttag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca taaagatcta gccgatcaga
      661 tcggatttta ccaaaatgct atcggagccg tacttggtcc ccaagatagc tggttacttc
      721 agcgcggaat caagacacta agtgttcgta tggaggaaca tcaaaaaaat gcttttgtgg
      781 tagctgattt tctattttct catccagcag tagaaaaggt ctactatcca ggattacccg
      841 atcatgaact ccatggagtt gctaagcagc agatgagcgg ctttagcgga atgatttctt
      901 ttactttaaa aaatgaagaa tctgctattc catttgttga atcactccaa ctatttactt
      961 tagcagaaag tttaggtggc gtagaaagtt tagtggaaat tccttctgtc atgactcatg
     1021 cgtctattcc aaaagaaaaa cgagaagagg ctggcataaa ggacggcttg atccgtcttt
     1081 ctgtaggaat tgagtatggt caggatctca taaacgattt agctcaggca tttgacagaa
     1141 tcaagaacta gaagtcccaa taaacgactt agcagaaaaa tctaaaaaat atttcagatt
     1201 gctacacctt ggaaacgata agtgttagaa tcaagtagaa agacgttttt gtaaatttca
     1261 ctaaataatt ctttagtgaa aagcatcaaa acgaatgaac aaacagatag gagtcttaca
     1321 aatgagaatg aatgtcgaaa gttttaatct tgaccataca aaagtcaaag caccttatgt
     1381 acgtgtagct gatcgaaaaa cgggtgcgca tggcgatgtc atcattaaat acgacgtccg
     1441 ctttaaacaa ccaaataaag aacacatgga catgccaagt ctacattcac ttgagcactt
     1501 gactgccgaa ctaatccgca atcacgcaga ttacatcgta gactggtctc caatgggatg
     1561 ccaaacaggc ttttacttga ctgtattgaa tcatgaaagc tacgaagaca ttttagatgt
     1621 tcttgaagca acaatgaaag atgttcttga agctactgaa gtacctgcaa gcaatgaaac
     1681 acaatgtggc tgggctgcca gccatacact agaaggtgca caagctttgg ctcgtgaatt
     1741 tttagcaaaa cgcgatgaat ggtctgaagt agaagcatag tttctaaaag aacgtaaaga
     1801 tgagaagcaa tatagctatc ttatcttcgt attttattaa atatttattg tctgaaaaga
     1861 gttctaaacg ggaaaacaaa tagctagttt tcagtttagc gctcttttta ttatataaaa
     1921 tagaaaggag aaaactttat gtctatttat aattttgaaa ctactttaga agacggcact
     1981 gtctattcat tgaaaaaata tgcaggcaaa ccgataatca ttgttaacac tgcaacaaaa
     2041 tgcggtctag cacctcaatt taaacaatta gaagaacttt accaagctta tcagacacaa
     2101 ggtctggtta tcttaggctt tccttccaat caatttaaac aagagctaga ttcagcgact
     2161 gaagcagcag aagcttgtaa aaccacttat ggcgttacct tccctatgca tcaattgatt
     2221 gacgtaaatg gagaaaatac agatcctctt tttacttatc ttaaaaatga agctcctggt
     2281 actgtcacta aagcaataaa atggaatttt actaaatttt tgattgatag aaatggcaaa
     2341 gtaatcgaac gatttgcacc acaaacttct ccattgaaaa tgaccgctgc aattgaatcc
     2401 gttcttta
//
LOCUS       DANKAZ010000196         2400 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000196 DANKAZ010000000
VERSION     DANKAZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.203"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            207..2069
                     /locus_tag="PWY67_002850"
     CDS             207..2069
                     /locus_tag="PWY67_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2059043.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="PWY67_002851"
     CDS             2069..>2400
                     /locus_tag="PWY67_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2059044.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DANKAZ010000197         2392 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000197 DANKAZ010000000
VERSION     DANKAZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(170..352)
                     /locus_tag="PWY67_002852"
                     /pseudo
     CDS             complement(170..352)
                     /locus_tag="PWY67_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            339..536
                     /locus_tag="PWY67_002853"
     CDS             339..536
                     /locus_tag="PWY67_002853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059045.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(579..1154)
                     /locus_tag="PWY67_002854"
     CDS             complement(579..1154)
                     /locus_tag="PWY67_002854"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL2059046.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1309..1835)
                     /locus_tag="PWY67_002855"
                     /pseudo
     CDS             complement(1309..1835)
                     /locus_tag="PWY67_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          735 a          438 c          379 g          840 t
ORIGIN      
        1 aaataatcct gtttttacag tgaattattt taattttgat tttcaataaa aatatattac
       61 aataataatc gtaatagcta atgaaacaat ttctcaatct ttttaccact ccaaacaggg
      121 agcgtcaata attttgtgta aataactcgt ccttctgcaa aataatgggt tactcagtaa
      181 acattgaagc taatgtatcc gtcacttgtt ggaagccttt atggcttcta tttaagaatt
      241 tttgattgta tgtatcaaag atggatacta ggaaacgctc tagtgattct tcattttgaa
      301 actgctcttt tctgcggctg tatttcttga tttgtttatt gaaagattcg attgtagaac
      361 cagaaggaat ggagaacact tttgaacttt acaacaaaac tactgttgat aaattaattc
      421 caattttagt tccaactgat ttaaatggtc atgatgtttt acttcgtttt aacggatttt
      481 taggtgagga ttatagatac ctaaaaattt ctgatcctca acctttaaca gaataataaa
      541 aaacgtgtaa tcaattattt gattacacgt tttttatttc aatcttcgtt gtagtagtgg
      601 ttgcgacata ccgaaaggta atcttcatta cctcctacta caatctgttc acctgaataa
      661 actggaatac catcaacaat acgagcattc attacagcct ttttattgca aaaatggcaa
      721 atagttttaa tttcctcaat tttatcagca aatgtcaata atgcttcgga tcccttgaag
      781 agattatttt gaaaatcatt cttcaatcca tagcaaatta ctggtacttc ctcattcatt
      841 acgataagtg atagatcaat aatattctct ctacttaaaa attgagactc atcgaccaaa
      901 atacaatcaa cttttccgta ctgatcaata atctccacta attctccgta cacatccatt
      961 tcagaagtaa ttgcaatagc tggtttcgaa acacctatac gactctcaac ttttccaaat
     1021 cctgatcgag tatctattgc gcttgtcatt atgataactc tcttcccttg ttcttcataa
     1081 ttatgagcca ctttcagtag ctcaatactc tttccactat tcattgttcc atatctgtaa
     1141 aataattgtg ccattaaatt tattcccttc ttttacaatt caattattcc ttaattccat
     1201 ctgcttttat gcagctgctc gtttattcct tgatatgtct ataacatcta ctatccctaa
     1261 aaccaattta taagccatta gagatggtat tcctgaacat ctctcatatt gttttttgag
     1321 tggtatatca tctgaaaaac tgaaggaatc atgaaaatag cgacatactt atcatttaga
     1381 gttctaactt taaatgcatt tacttcttca gtcagcactt tagtcatgtt ggacatggtt
     1441 tggggcgtgt agtgatgccc atacattttc tcaatcaagt ctgcgatttc cgacatggta
     1501 ataccttttc ggaagaggtg aatgacggtc tcctctaatg tgtcattcgt ccgtctataa
     1561 gcaggaacag tctgttgctt gaactcgcca ttgcggtcgc gcggaatctg gagatgaagt
     1621 tccccatact cggtcttgac cgtacggtca taggagccgt tacgagaatt acccgtgtta
     1681 aaaccaatgc gatcgtactt ttcgtaatcg aggaaagccg ttaactccgt tttgagaagc
     1741 gtattgacag ctttttccaa atggacacgg aataatccat tcaaatcgcc tttgttcgct
     1801 agagtcttta ggatttctgt agtaaaatcg ttcatatgga agtcctcttt tctgtgaatg
     1861 tgttgtggta actctattct acagaaggga cttccttttt tctatttaca caaaatattt
     1921 tacactctca acaaaagtat cttcgattaa tacttgctca agttatgtgc agctacgaaa
     1981 aagtaggtac cgaggtactt tctttttcgc agcttttttt ggacttatga gaagtacatt
     2041 cgctggaaga atacgatata aaacgagctt tttcactaaa gatattaata gagtgaaaga
     2101 aacaagcttt tgttgtcagt tttttcactt ttctaaaaat gaaaataaaa aattctggaa
     2161 gtggagaaga attttgcttg ctatactttg tcttgtagaa aagaggtaga caaaataatg
     2221 caagctcaaa taagcgattt tccataacct tggtaggaat gacaaattaa ataggaaagg
     2281 gaagtaataa tatgaataaa aaaaagttat ctctattcgg tttttttgcc ctgacagctt
     2341 ccatggtttt gactgtttat gagtatccga cctttgcaac atcacaatta ca
//
LOCUS       DANKAZ010000198         2246 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000198 DANKAZ010000000
VERSION     DANKAZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.205"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(45..2129)
                     /gene="cadA"
                     /locus_tag="PWY67_002856"
     CDS             complement(45..2129)
                     /gene="cadA"
                     /locus_tag="PWY67_002856"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2059047.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          648 a          510 c          355 g          733 t
ORIGIN      
        1 tttacatttt tcattaagaa aaggatataa aaaaaagagg acgttcattc gtcctctttt
       61 tttacatgca agcttcgcat ggcattcaaa acagctaata ctgtcacccc aacatctgca
      121 aatactgcat ctcccattga tgcaaaacca aaagcaccta aggcaagtac caaaattttt
      181 atcccaatag caaagacaat gttttgtttg acgattttca atgttttctt tgctaaatgg
      241 attgcttctg caatcttcga tggttgatca ttcatgatga ctacatctgc tgcttcaatt
      301 gcagcatcgc ttcccaatcc acccattgcg attcctacat ctgctcttgc tagcaccggt
      361 gcatcgttga ttccatctcc gacaaatgcg gtctttttat tgtttctttg taagatttcc
      421 tctaaccgct gcactttatc ttcaggaagc aattcgctat atatcttgtc cacgcctact
      481 tgcttcccga tatgatcagc aatttttttc gagtcgcccg tcaacataac tgtgttcttc
      541 acaccagcct gttttaattg aaccaatgcg tctacggcat cttctttcaa ggtatctgca
      601 ataacaagat aaccactgta actttgatcg atcgctagat aaagcaatgt accgatttct
      661 tgacttaaag gaaaagagat ttcaaatctt tccatcaatt tgtgattacc tgctaatact
      721 tgtttgccat caacttcagc taagatccct tcacctgcaa attcctggat attagttgtt
      781 ggtaaaagcg gacctgcgta gccttctaaa acagattgag caatcggatg tgtagaaaac
      841 tgttctgcac tcgcaatata ttgcatgaat ttctttggat cgatttttgt atcgatcgtc
      901 tggatcgaaa agtcaccttt cgttaatgtg ccggttttat cgaataccac tgtctcggtt
      961 tgcgcaagca actccaagta atttgaacct tttactaata caccgatttt acttgcgcct
     1021 ccgataccgc caaagaaact aagtggcacg gaaatcacta atgcacatgg acatgagatc
     1081 actaaaaatg tcaatgctcg atagatccat tcagaaaacg ccgctccaga aacaacaagt
     1141 ggaggtaaaa cagctaataa aacggccaat cctacaacga ctggtgtgta atagcgcgcg
     1201 aatttagtaa tgaaattttc agctggtgct tttttactgc tggcattttc tactaattct
     1261 aataatttgc tgatagtaga attttcataa gatttttcta cttgaactaa aaatggttta
     1321 tttttattga taaatccgct taatactgtt tcaccaacat atacagattt agggactgat
     1381 tctcctgtaa gtgctgacgt atcgactaat gattctcctt cgatgatcgt tccatctaac
     1441 ggtacacgtt caccaggctt cacaagtaca aactgaccga tctgcacgtc ttcagggttc
     1501 acggtcatca atccttccgt cgtctgcacg tgagctgttt ctggacggat cgccatcaat
     1561 tcgcgaatcg attttcttga atgactgact gccaatcctt ggaaatactc gccgatttga
     1621 taaaatagca tcactgctac ggcttctgga tattcaccga tcaacatagc gccaatcgta
     1681 gcaacagaca taagaaaatt ctcatcaaag atttttcctt tgacgatatt catcactgcc
     1741 ttcttcacta catcatatcc tatcagcaga taaatcaata aataccctat caagcgaatt
     1801 ggttcattaa aatcaaataa tagcaataaa gcgatacctg caagggaaaa taaaatccgg
     1861 aacagcgtat ttctctcttt ttcatttcca tgttcagata ctttttcctt atctatttct
     1921 gttactttga catctggttc taattccttc actacttttt ttgtttcttg ttcaatcgta
     1981 gccaaatgat cttcctctgc gtcaatcgtt agttttccag aagtgaagtt taccgttgct
     2041 tccttgactc cattgatctt ctgaactcct ttttcgattt tcattgcaca gttcgcacaa
     2101 tctaaacctt ctaatctgta acttttcatt ttaatgacct cctcgcctca acatatgaac
     2161 atctcttcat aactaattat aaatgaatgg ttattcatat gtcaaggtat ccgaaagaaa
     2221 aaatgaaaaa catctgcatc ggaaaa
//
LOCUS       DANKAZ010000199         2243 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000199 DANKAZ010000000
VERSION     DANKAZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..177
                     /locus_tag="PWY67_002857"
     CDS             <1..177
                     /locus_tag="PWY67_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059048.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="PWY67_002858"
     CDS             190..690
                     /locus_tag="PWY67_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2059049.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..>2243
                     /locus_tag="PWY67_002859"
     CDS             787..>2243
                     /locus_tag="PWY67_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL2059050.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIED"
BASE COUNT          747 a          294 c          498 g          704 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga ttt
//
LOCUS       DANKAZ010000200         2226 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000200 DANKAZ010000000
VERSION     DANKAZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.207"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            200..742
                     /locus_tag="PWY67_002860"
     CDS             200..742
                     /locus_tag="PWY67_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059051.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="PWY67_002861"
     CDS             898..1347
                     /locus_tag="PWY67_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL2059052.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2226)
                     /locus_tag="PWY67_002862"
     CDS             complement(1377..>2226)
                     /locus_tag="PWY67_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HDL2059053.1"
                     /translation="SISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAY
                     GTRKLKKCLAKRGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRT
                     FTQEQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRI
                     PYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVE
                     FVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSD
                     TSGEAA"
BASE COUNT          780 a          362 c          428 g          656 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatccg cggcgtttca tgattcgacc gattcggcgc
     2041 cgactgagtt gaagcccacg ctttgctaaa cacttcttca attttcgggt accgtaagcc
     2101 tttcggtttc gaataaactc ttcctgaacg atctcttcta agtcggactc attttcgatc
     2161 ggtttcgctt gataatagta ggtctgacga gaaatattta gaattttgca catcgctgat
     2221 atggaa
//
LOCUS       DANKAZ010000201         2133 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000201 DANKAZ010000000
VERSION     DANKAZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            18..>2133
                     /gene="acm"
                     /locus_tag="PWY67_002863"
     CDS             18..>2133
                     /gene="acm"
                     /locus_tag="PWY67_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL2059054.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          840 a          343 c          416 g          534 t
ORIGIN      
        1 taggggagat agaaaaaatg aaaaagtgta gaaatattat tttatcaatg ttatttatta
       61 ttactaatat aacatcactg attcctgttc atgtctatgc ggatgcaggc agagatatca
      121 gcagtaatgt cacttcgttg accgtggacc caacgaatat tacagatgga ggaaacataa
      181 aagtaaaatt ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat tatctttgga
      241 tcaattggcc aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa atcccattaa
      301 tgattgaaaa taaaaatgtt ggaactttaa cggtcagaaa agattctgcg caggttgtgt
      361 ttaatgaaaa tattaaaaac ctagactcag tagaaggttg ggggcaattt gagatacagg
      421 ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc attcaccgtg acgagcggtg
      481 ataaaacagc tactgtaaat gtgacaaagc cagcttctgg ttcttcttct agcgtttttt
      541 attataaaac aggagacatg ctacctgaag atactaagca cattcgatgg tttttgaata
      601 ttaacaataa cggcacttat gttgaacaac cagttaaaat cagtgatgaa atccaaagtg
      661 gacaaagatt agatccaagt acgtttgaaa ttaatcaaat acacttaggt gaacagaagg
      721 tttatagagg agaagaggga atccaacaat ttttacaaga tttccccagc gctacattta
      781 atttttcagt aactgataat tacattgaaa taactatacc aaaaaacttt gtcaatctta
      841 ggaaaataat ggtgagttat aagacgatca tagaaaaccc agaacaaata aattttgaga
      901 atcattcgga agcttggttt aaagaattta ataaacctgc tgttgatgga gaaagtttca
      961 atcatacagt taaaaatatt tcagcttctg gtggagtcaa cggtacagtt agaggcgaat
     1021 tgaagatttt caaatatatc aatgacacag agattggtat cccaaatgtg actttcgaat
     1081 taagacgagc agatgaacag cctattcaag gacagtctag tatcctgttg acttcgaatg
     1141 aacagggaga agctagtatc aaaggcctgc aagtaggaga ctatgttgta aaagaaaaag
     1201 aagcacccaa ttggattgat tttgatccac taagttcaaa tgaactcaaa ttctccataa
     1261 acgaaaatga tacagaggga gtcagtttac ctatttataa caagaaaaag gttacgaaca
     1321 tcacagcaac taaaatttgg aacggtggaa ccactccaag accgagtatt tattttaaat
     1381 tattcagagc aagtacaaac aattgggaac cagttcctga tgcagaaaca aaacgattag
     1441 acaatggaat aacttcagtg acttgggaag atatccaaca gtatgatgat agtggaaacg
     1501 aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc ccttcaggtt
     1561 atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc atttcagtct
     1621 caggacaaaa aacatgggaa gacaatgaga accaagacgg caaacgaccg acgacgatca
     1681 cagtaaatct attagccgac ggtcaaccga tccaacacaa agaagtaagt gaaaaagatg
     1741 attggcgcta tcgtttcaca aatctgccaa aatacagaga cgggcaagaa atcatctata
     1801 ctgtaacgga agacaacgtg cccgaataca gtacgacgat tgacgggtat aacatcaaaa
     1861 acagttatac accggaaaaa acaaatatat ctataccagg tagccatata acaccgtgga
     1921 aaaattttcc agagaagaca gagagagaga aaattagaaa tctattctct caaaaattgc
     1981 aaatatttgc tcatacagga gaatccagcg aacaaagaag caattatatt agtaagagag
     2041 ctgtacctaa acagataaca aataaatcaa aaagcattct tcctaaaaca agtagtaaaa
     2101 aatctagttt tgctgtaatc attggtttat tga
//
LOCUS       DANKAZ010000202         2094 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000202 DANKAZ010000000
VERSION     DANKAZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..153)
                     /locus_tag="PWY67_002864"
     CDS             complement(<1..153)
                     /locus_tag="PWY67_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2059055.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="PWY67_002865"
     CDS             complement(475..696)
                     /locus_tag="PWY67_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059056.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="PWY67_002866"
     CDS             complement(794..1039)
                     /locus_tag="PWY67_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059057.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="PWY67_002867"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="PWY67_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          699 a          370 c          305 g          720 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aaga
//
LOCUS       DANKAZ010000203         2058 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000203 DANKAZ010000000
VERSION     DANKAZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.210"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..337)
                     /locus_tag="PWY67_002868"
     CDS             complement(<1..337)
                     /locus_tag="PWY67_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2059058.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="PWY67_002869"
     CDS             complement(404..559)
                     /locus_tag="PWY67_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2059059.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="PWY67_002870"
     CDS             1176..1397
                     /locus_tag="PWY67_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059060.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="PWY67_002871"
     CDS             1398..1655
                     /locus_tag="PWY67_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059061.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="PWY67_002872"
     CDS             complement(1791..>2058)
                     /locus_tag="PWY67_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2059062.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DANKAZ010000204         2021 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000204 DANKAZ010000000
VERSION     DANKAZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            635..967
                     /locus_tag="PWY67_002873"
     CDS             635..967
                     /locus_tag="PWY67_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL2059063.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1586..1927
                     /locus_tag="PWY67_002874"
     CDS             1586..1927
                     /locus_tag="PWY67_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDL2059064.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          702 a          271 c          428 g          620 t
ORIGIN      
        1 caaatataga aaagggaacg tatgtttgca tactcctttt ttttgagtta taataatacg
       61 cgaagagaga taatttcatt atttaaacaa gctaaaaatc ttgatattac ttcttttcag
      121 gcaatacgaa tattattcgc ttacattttt tgtataaatt taattgtctg ctatgcttgt
      181 gatgaattga ttttcgtttt gcacttttgg atgataaatt tggacaattg aaaatacaaa
      241 aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt cgtcagcgtt catacacggc
      301 attaagattt ggcccttaat gcgttaacct ttcagtgtta cggtattacc cgcaatggat
      361 ttagtataac agatattcaa aagtgatacc actttttttg gctgtgttaa ggttaaaaat
      421 acgtgatatt acgatgaatc tatgcctaag cataatgtta taaaaatgtc cgtgtggctc
      481 tttgccgtat gacgaacgct gaaattagag ggaacgcatg tttggatgcg ttccctcttt
      541 tttgtttgtc atgaacgaaa atcaattctc tatttttgaa aaataacaag aaaaacttgt
      601 taaaaatatt taaagaaatt gggtggttta ttttatgtca tatacaggaa ttttgagtct
      661 caaagatatt tgtcactacg gtaaacggtg taccgctaca gagaaaatca cgaagaaact
      721 atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc agaaagacaa
      781 agtctcagag gaaatgattt attacattgg gaaacaaaag caaataattt taaaagatcc
      841 tatcccctta aaagagctat atccaacaat caaacatgtt tatgaccaaa acggtgtact
      901 gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg ggcgtttgat
      961 cggctgaatt gatcattctc ataacttgta ttttatcgtt tttcgcttgc ttgaaaatat
     1021 tgtcgagact tctaaaggaa cttagagtca ctagaaaaat aaggagtaga cttgatctat
     1081 caacaattcc cttcggggag tgacggggta ggttttaacg attggtaacg taaaaaattg
     1141 gtcaatcgag taaatgcgct tgtttggata gtggtgaaat gaatttgtgt aaataataag
     1201 cgcagagtaa acaagaggga atttagaagt tagacaagtt agaatagcgt ctagattaat
     1261 catgagcagc tactaaggga tctagacttt gacgcaaact catggaataa ggagtaggtc
     1321 ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac atgtagatac
     1381 ttgcgaggac gttgattatt ggatcaatcg agcatttacc gctgtctact agggtttctc
     1441 gtttcggacg ccataaacga gtcgaaattt tttatgtatt tttaagtaaa cgaaaaacga
     1501 taaaatatga acaagtcctg aataaagtga aaaaattaat ataaagcata gagaaaatga
     1561 aatttacgta atggaggaat gacaaatgaa aaattatgat ccgaatatac gattgggcac
     1621 acatactatt aaggtttctt tccaacgttg ggattacaaa ggatttgtga cgtttagaag
     1681 gggcggaaat tgtaaaggat tagatgtttt agcattagac gaggatgatt tatacgatca
     1741 aacattgact gataatccaa ttggattcgg cttgctacct gaagatgatg aaggtaatga
     1801 atggtttaaa atgacgctta tgaatgataa tggagacgaa ttatctgttg aagatacttg
     1861 gtcatatttg agtgattata ttgtcagctt tgaaatcatt gaatttgtag cggataaaga
     1921 agaataaaaa ctggaggaat aaattttaca gataaaggct tagaaggagt cacgacgatt
     1981 aataaaaagg tagtattacc ttaggcattg agtaaggaat t
//
LOCUS       DANKAZ010000205         2011 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000205 DANKAZ010000000
VERSION     DANKAZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.212"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(387..1853)
                     /locus_tag="PWY67_002875"
     CDS             complement(387..1853)
                     /locus_tag="PWY67_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2059065.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          633 a          405 c          336 g          637 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt t
//
LOCUS       DANKAZ010000206         2003 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000206 DANKAZ010000000
VERSION     DANKAZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.213"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..378
                     /locus_tag="PWY67_002876"
     CDS             <1..378
                     /locus_tag="PWY67_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL2059066.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="PWY67_002877"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="PWY67_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2059067.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="PWY67_002878"
     CDS             1859..>2003
                     /locus_tag="PWY67_002878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059068.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DANKAZ010000207         1993 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000207 DANKAZ010000000
VERSION     DANKAZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.214"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="PWY67_002879"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="PWY67_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2059069.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="PWY67_002880"
     CDS             852..1040
                     /locus_tag="PWY67_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2059070.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="PWY67_002881"
     CDS             1053..1610
                     /locus_tag="PWY67_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2059071.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="PWY67_002882"
     CDS             1642..1884
                     /locus_tag="PWY67_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2059072.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          555 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ctt
//
LOCUS       DANKAZ010000208         1786 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000208 DANKAZ010000000
VERSION     DANKAZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.215"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="PWY67_002883"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="PWY67_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2059073.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          499 a          307 c          352 g          628 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagt
//
LOCUS       DANKAZ010000209         1747 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000209 DANKAZ010000000
VERSION     DANKAZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..184)
                     /locus_tag="PWY67_002884"
     CDS             complement(<1..184)
                     /locus_tag="PWY67_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2059074.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEI"
     gene            complement(181..834)
                     /locus_tag="PWY67_002885"
     CDS             complement(181..834)
                     /locus_tag="PWY67_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2059075.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1122..>1747
                     /locus_tag="PWY67_002886"
     CDS             1122..>1747
                     /locus_tag="PWY67_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2059076.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          557 a          318 c          237 g          635 t
ORIGIN      
        1 ttatttcttt tccattcata ttactagaaa tttcatttgc catattatga atcttttctt
       61 ttctcgtctc caaatatgtt ttaggaaaga taaaaaaaat taatgaatga actaatataa
      121 ttattattcc aagaacagaa aatatttgta tgaacatttt tggaaatatc tttaattttt
      181 tcattttctc tccaatttat atcctacatt tcttatagta gttatacagt ctaattgtaa
      241 tttttttcta agttctttta tatatacatc tattactcga tcataaggag tttcttcgct
      301 atctttccat acataatcaa taatttgcat tcttgttaac acttgtccat tattatccaa
      361 taaacatttt aatacttcta gttcttttgc atttacatct atttcttcac catttatttt
      421 agctgtatag ctattaaaat taaccgatag atccttatat tcaaacttct ctaaatgtcc
      481 ataattcttc ttaattaaag aatctattct agcttttaag accggcaatg aaaatggctt
      541 ttctacatat ccgtctacta aattagtaaa tgcatcaatt ttatattctt catcactaaa
      601 tgcagtcaag attagaattg gtaaattgct tttctttcta atttctttca acacttctaa
      661 accatttata aaaggtatct gaatgtctaa gataaccaaa tttatatcgc tattaaattt
      721 tgacaaggct tctcttccat ctttagcttg aataacagta tatccaaatt ctgaaagata
      781 ttcacttatt ccctctctta tcatattatc atcttcaaca gttaatattt tcatatatac
      841 ctccacttaa gcctatacac tagatattat acagcatatt atggctctat aatatctagt
      901 gttggtgaca ccttctgttg ccaatacttt ttcttttttc taataaatgc aaacagacac
      961 cttgaaatcc tttagaataa agggttctat aatattttgt gttgttggaa aatcgaaggc
     1021 gattttctac taacactttt tcttcgtttc aactcaaaaa gagactcact tttggtaaaa
     1081 tttagtcgac caaaacatca ccctaccaaa ggagtcgctc tatggataat catactagaa
     1141 aattactcgg attaacagat aaacatttat tttttgatga agaatggctg attgaaaaag
     1201 aatataaagg cgttcaagcg cagtttatta aagggagatt ggatgactca ccttcccatt
     1261 gtgaacactg tggatcgatt cgtattatcc gcaacggttc ctatacgaca cgtactcaga
     1321 tattgaaagt caaagaaaag ttgactattt tagaattaaa acgtacccgt tttctatgct
     1381 atgattgtgg gcagaccttt tctgcgaaaa cggacttagt tgatgaacat catcaactaa
     1441 caaaggaact gaagcaggcg attctcatag aactgtacga aaatcaatca cgtaaactaa
     1501 tcgctaagaa gtacttcgtc tcggatggga cggtgacacg gatcttgcgt gaagcaacga
     1561 agcattacca gccacgtatg aacttccttc cgacggtgtt gtgtatggat gagttcaaat
     1621 caatgaagtc tgtttctggt tccatgagtt ttatttgtgt tgatgggaca acccatcagc
     1681 tatttacgat ccttgaagat cgcagacttt acaaactaac tcaatacttc ctgcgctttc
     1741 ctagaaa
//
LOCUS       DANKAZ010000210         1622 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000210 DANKAZ010000000
VERSION     DANKAZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            1..117
                     /locus_tag="PWY67_002887"
                     /pseudo
     CDS             1..117
                     /locus_tag="PWY67_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          614 a          189 c          327 g          492 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacga ttatcgaaca
       61 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaattt aatttgacaa
      121 ttaagaaagt attaacttgt tttgtgaaaa agagaatttg aaaaacgatt ggcgaacaaa
      181 tgctcggtag gtatggtata aatacagagc taattggtgt gatgaaatca gatattcgcg
      241 tgagattcag taaagtagga gttcgaaaac agctaggtga caaacagtat tgctttggta
      301 cttcggaatg gtttatcaaa ttcttatcaa cagttcactt ctctttatgt ttgagtgatg
      361 attcgttagg ttattgcttg gaaagaccgg atgagagtag aagagtttaa ttttataaag
      421 tgaaaagagg taatgtacga tatacgtagg gctgaaccaa tagaggataa gcaaacgatg
      481 ttacaggcgc aaaattcata gcttactgtt ttggagataa cagaagagga aataatgcag
      541 aagtaaggcg aaaaaaggac ggaagcctaa cagcatatga aataaagaaa aatattataa
      601 ctactgataa atcggtattg agcaaagtac taataggagt caaaaacatt cataagctat
      661 gagtgtttct tggctacttt tttattttta tgtatctaca aaagtttcca ggggcagaac
      721 ctgtgattat taatggaaga acctcaagta ttgtcttaaa tcattgttat gaaaatggat
      781 taaaagagtt catcaaggtg tgattaacaa gttacttttc attacatcga atttttcaga
      841 agttgcttat attggtgatg atattaatga tttatcatgt atgacatctg tgaagaagcg
      901 ggaggaatag tagaatgtcc aaaaaatgcc acaagaaatg agattgaatt atctgatttt
      961 attgtaactc acagtggtgg tgacgaatct gtgatagagt ttattgagtg gatattggaa
     1021 tcgatatagt gtagtagtac agaaaaatta tcatacaatt aatactaggc ataagaatat
     1081 tggcatattc tagagttaaa agtaactatt cgaaaaggat aaggttagaa ttttttgttt
     1141 tgccagtgga agaatataaa aagggcattg gaaattaata tattagaatg ctaagcggag
     1201 aaaaaaatat atgaaaagta cgtccttgtg atgggatgga aaaatacact tatttcaaac
     1261 aacgataata taattatgtg aaccgaagta tactcgcttc taattcaatc ctcaaatccc
     1321 gacagtcgcg acttcaaaaa agcgtatagt tttaatgtat ccccatatga tccgaaaaat
     1381 ggtaatttac aattaacagt ttgatgatgt tgaagatgtg gaaatgctga aattaccatg
     1441 atgagaaaca cattaattaa agggaattaa ttaactagaa tataatctga ttcaatttga
     1501 attaagcaaa aaaaggctga gttttaaata atcgcgaatt gtaaaattaa gttagaaaaa
     1561 taaaaaggca tttatggtac actcaaattg tatttccgac gaaagaaaac aaaggagtgt
     1621 aa
//
LOCUS       DANKAZ010000211         1593 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000211 DANKAZ010000000
VERSION     DANKAZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            424..1464
                     /locus_tag="PWY67_002888"
     CDS             424..1464
                     /locus_tag="PWY67_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2059077.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          626 a          204 c          243 g          520 t
ORIGIN      
        1 attttttagt agtatataat ctattgtttt ctagcctaaa gccttgtctt ataagaataa
       61 tataatatat actactgaaa taaggtagta tatattacta gtaatggtag tatgttttat
      121 atgcataagg tagttcttaa tttatttttt gttttgacac tggtagtact taattatctt
      181 taattttaaa actaaatttt attcatacgt ttttctggta gtatttaatt ataaataata
      241 tttatttaaa gtagttctta atgactttaa aggtagtaca taattcattt tatttctgat
      301 tttaggtagt atttaattaa aaaacagttt taatgcgttt gcatttttta tttatctttg
      361 ctattttcga aaagagtagg taattttaca taaataaaac aacgtgaaag tagaggatct
      421 acaatgactg attttaaatt ttttaaggcg gatagagttt acaacgaatt attttatcaa
      481 tttccaaaag tctttattgt ttctgacgaa tacaaaaaaa tgaaagattc aactaagatt
      541 gcctatatgc ttttaaaagc aagattagag atcgcaatca gcaaacggca aatcgatgaa
      601 gaaggtaatg tttattttac ttatacgaca aatgaactat gtagagtatt aaactgccaa
      661 aaacaaaaag cgatagcaat caaaaaagag ttggaatcct ttggtttatt attacaaaag
      721 cagatgggat ttaacaaaca gttagggaaa aataatccta atagactata tctagcagaa
      781 ttaaaagtct cagaaaatga tatctactta ctcgaaaaat ttgatagaga gaatagggaa
      841 aacgttgata aatcagaggg tatgaaaatc atacccaccc tcgacgaaaa atcagacgct
      901 gaatcccttg gggctcaaga gggtatgaaa atcataccgt gccaaaacgt tgataaatca
      961 gagggtatga aaatcatacc agaacttaat aataatatat tagacactaa tagacacaat
     1021 atagacactg aaaaagaccg cctacaagat caattgttgt tagacaattt tgagacaatt
     1081 atgacaaacg acagcattgc tacgtttgtc cctgaacgag tattaaattt gataaaaaca
     1141 ttttcttcaa gttacagtga agctcaaaaa accgtccaga ctattcataa tgcaaagaaa
     1201 aaagctgaaa tagaaagtga tatttcgata gtttttgaag aactcgatag ttattatgtc
     1261 aatgcagaac aagaattata cacgacactg ttaaaagcct atcaaaaatt aaaaaccgaa
     1321 aaagtcgaaa atatccagaa cctgattttt gtctatgtaa aaaattggtt tatcgaaaaa
     1381 ccaatagctg ctaaagtatc aagtgaaaaa cgtttgaatt atgcaagctc cccaagcact
     1441 attacgaaag actggttaga gtgaaacttt actgacaaag tgattgttgc ttcatggagt
     1501 aacattaaaa ttaaaaattt tttattattc taattatatt acgaatatta caaaataata
     1561 gctattttaa tagaatttat aagttaaaat att
//
LOCUS       DANKAZ010000212         1590 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000212 DANKAZ010000000
VERSION     DANKAZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.219"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            80..1546
                     /locus_tag="PWY67_002889"
     CDS             80..1546
                     /locus_tag="PWY67_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL2059078.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          443 a          291 c          332 g          524 t
ORIGIN      
        1 ttaaataagt tttttagtta aatttttcgg taaagatgta taatattttc tgaaaattta
       61 aagaattggg gtatagatta tggaaaaaca gcaagacaag ggcttcttcg ggcagccgaa
      121 gggattgtca actttgttct tcactgagat gtgggaacga tttagttatt acggtatgcg
      181 tgcattactt atctattata tgtacgatac agttgccaac gggggacttg gattaccacg
      241 agcaacagca ttagctatca tgtcgatcta cggatctttg atctacatgt ctagtatcgt
      301 cggcggatgg gtatctgacc gagtgttagg agcaaaacgc actgtattct tcggtggagt
      361 tttcattatg cttggccaca tcgtcttggc tttaccattt ggtgtaagta cattgtttgt
      421 ttcgatggca ttgattatta tcggaaccgc ctttttgaaa ccaaatgttt ctggcatggt
      481 cggacatctt tattcaaaga ctgacttgcg aagagatgca ggattttcga ttttttacat
      541 gggtgtcaac ttaggggcat tattagcacc actaatcgtt ggtacaatcg gtcaagaata
      601 taactatcat ttagggttct ctttagctgc tattgggatg ttctttggat tattacaata
      661 catcatccaa ggacgcaaga cattagacgg agtgggtaca acaccgccga atcctttgac
      721 aactgaagaa cgtaaaggat tcatccgtaa cttgatcatc gcacttgttg ttatcgctgc
      781 agtattcggc ggggcacaac taactggtca tctgacaatt gacttttttg tcaatacaat
      841 cagtgtattg gggattatct taccgttgta ttactttatc aaaatgctga cttcaaaaga
      901 tgtaactgct gaagaaaaac caagagtatg ggcttatatc ccattattcc ttgcagcaat
      961 cattttctgg tcattacaag aacaaggttc atctatctta gctttatttg ctagtgaacg
     1021 tacacaatct acgctatttg gtgtaacgat ccaagcaagc tggtatcaat cattgaatcc
     1081 aattttcatc tttattctga caccagtatt cgtaacgatg tggacgaaac ttggtgaacg
     1141 tcaaccatct acagtagtga aattctctct tggactttta tttgcaggat tatcgtactt
     1201 gctattgatg ttgccgggta tgctttatgg tacaagtgga aaagtaagtc cattgtggtt
     1261 gttgggaagt ttcttcatcg tggttattgc tgaattgtgt ctttcaccag ttggtttgtc
     1321 tgtgacaaca aaattagcac cacgagcatt tgaatcacaa acaatcgcta tttggttctt
     1381 agctgatgct tcttcacaag caatcaatgc acaaattgca cgcttctaca caccgggaac
     1441 agaatctgct tacttcggta ttatcggtgc agtagcaatc gtcggtggta tcttgctatt
     1501 tactgtgaaa gaaccaatca aaaaactaat gggctctatt cattaatcaa tgaacataaa
     1561 aacaggaatt gcttatttgc agcagttcct
//
LOCUS       DANKAZ010000213         1575 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000213 DANKAZ010000000
VERSION     DANKAZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.220"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(943..1356)
                     /locus_tag="PWY67_002890"
     CDS             complement(943..1356)
                     /locus_tag="PWY67_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL2059079.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          592 a          251 c          230 g          502 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaat
//
LOCUS       DANKAZ010000214         1551 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000214 DANKAZ010000000
VERSION     DANKAZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            132..344
                     /locus_tag="PWY67_002891"
     CDS             132..344
                     /locus_tag="PWY67_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059080.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="PWY67_002892"
     CDS             506..1024
                     /locus_tag="PWY67_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDL2059081.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="PWY67_002893"
     CDS             1031..1543
                     /locus_tag="PWY67_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059082.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          611 a          181 c          241 g          518 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt a
//
LOCUS       DANKAZ010000215         1539 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000215 DANKAZ010000000
VERSION     DANKAZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.222"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            17..1072
                     /locus_tag="PWY67_002894"
     CDS             17..1072
                     /locus_tag="PWY67_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2059083.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT          620 a          143 c          290 g          486 t
ORIGIN      
        1 agaggtataa aaagttatga aacacaaggt aagtgtgata gtaccaattt acaatgttga
       61 aaaatattta agaaaatgtg tggatagttt attgagtcag acattaaaag agatagaaat
      121 cgtattagtt gatgatggtt cgccagatgc ttcgggggaa atagcagatg agtatcaaaa
      181 aaaatattct aatgtaaaaa caatacatag agaaaatggg gggttaggac cggcaagaaa
      241 tacaggaatt gagaatgcga caggagaata tgtggctttt cttgattcag atgattgggt
      301 agaaagtgat atgtatgaaa aattatactt agttgcgtcc aaagaaaatg ctgatatagt
      361 agttagtgga cattgtgatt ttgcgaatga caaagctatg gtgaaaaagc aacatccttt
      421 gagtgggaat gtatatgata cgcctaatag tattaaaaat gtacgaaaaa atttatttgg
      481 acatagtcca aaagatcaag aagtagaggc cttcccgatg tcagtatgta tgtctttgta
      541 taataaaaat ctgctagata aatatagact aagatttgaa aaaattttat ctgaagatac
      601 aatttttaac ttgaatgcat atgatgtcgc aaataaaatt gcttttacag aatatacaga
      661 ttattgttat agaaaagaag aacaagagtc tattacaaaa agtttatctc caaataagca
      721 gacggaattt aaagaatttt taataactct aaaagctata gcatcgaaag aagatttaga
      781 atgtcaaatc agagcaaaac gtatggcaat tgattattgt aggctttatg tgggcttagt
      841 tgatgatgca aatttgggaa ttaaagagaa aagaaagtat ataaaaaaat ttattgaaga
      901 tgagggatta attcaatgtt ggaagggata tcctacaaat acgctaccat ttcaacaaaa
      961 attttttcat aaaatgcttg aatgtaagtg ttatacaata ttgttgttgt taagtagaat
     1021 tagaaagata attaaaaata tgaccagaaa aaaattgttt ataatattat aaactaaaag
     1081 tatatcaaat aatagaaatt aacaagttac aaaaaaatat tataaagaat aatattttct
     1141 aaaaagtaaa ctatagtaat gtggattatc accttccaac agtatataaa atgagcgtaa
     1201 aaacggctat ttttgcctta acaacgattt ttttgtatac aaatacgata tgctcattaa
     1261 ttattggaat ttgtagtgct gtttaaatta atgaaggtgt aattatatga gtgatcattt
     1321 tgaaaaagtt tgttgagaag atgtgcttct agtcatgtga tgggagaaaa taatttaact
     1381 gttagagtga atagttaaat tattctggtt atatgaaaaa agtagtttct aaaataatct
     1441 tgcagggatt aacttaaaga aattgagaga atagtttctt tgaaatttct atatacaatg
     1501 agaagctgaa atttactttt atttgatgac tggaaatag
//
LOCUS       DANKAZ010000216         1500 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000216 DANKAZ010000000
VERSION     DANKAZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.223"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            316..570
                     /locus_tag="PWY67_002895"
     CDS             316..570
                     /locus_tag="PWY67_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2059084.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="PWY67_002896"
     CDS             571..888
                     /locus_tag="PWY67_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2059085.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          531 a          228 c          290 g          451 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
//
LOCUS       DANKAZ010000217         1470 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000217 DANKAZ010000000
VERSION     DANKAZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.224"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            105..1406
                     /locus_tag="PWY67_002897"
     CDS             105..1406
                     /locus_tag="PWY67_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2059086.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          483 a          296 c          268 g          423 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat cagtcctatt tgacaaagag
//
LOCUS       DANKAZ010000218         1451 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000218 DANKAZ010000000
VERSION     DANKAZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.225"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..625)
                     /locus_tag="PWY67_002898"
     CDS             complement(<1..625)
                     /locus_tag="PWY67_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDL2059087.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="PWY67_002899"
     CDS             complement(784..1050)
                     /locus_tag="PWY67_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2059088.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="PWY67_002900"
     CDS             complement(1040..1396)
                     /locus_tag="PWY67_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2059089.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DANKAZ010000219         1451 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000219 DANKAZ010000000
VERSION     DANKAZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.226"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..650
                     /locus_tag="PWY67_002901"
     CDS             <1..650
                     /locus_tag="PWY67_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HDL2059090.1"
                     /translation="QVAESFEKKHQHVLRDIDALKKDVSNFGQMFVEGNEPDSYGRNR
                     RVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKNTVPQTIEDMMIYQLE
                     EMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGKESRAYEEISKKVFSKFW
                     SEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQLNRQQRLFGDDNE"
     gene            828..1169
                     /locus_tag="PWY67_002902"
     CDS             828..1169
                     /locus_tag="PWY67_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059091.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1162..>1451
                     /locus_tag="PWY67_002903"
     CDS             1162..>1451
                     /locus_tag="PWY67_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2059092.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          542 a          216 c          306 g          387 t
ORIGIN      
        1 tacaagtggc agaaagcttc gagaaaaaac atcaacatgt tttaagagat attgatgcac
       61 taaaaaaaga tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa ccagattcat
      121 atggcagaaa tcgacgagtt ttcttcatta gtagagatgg atttttcttg ctggctatgg
      181 ggtttacagg aaagaaagct atctacttca aacaaaaata cattgaagca ttcaacgaaa
      241 tggaagatgt tattcgcaag aatactgttc ctcaaacaat tgaagatatg atgatctatc
      301 aattagaaga aatgaaagat gttaaaaaag atgtttccat gcttaaagat actatgcgaa
      361 ttagcggaca acaagagttt gaaattaagc aaaaaggaaa tatgaaagtt atgggagttc
      421 tagggggaaa agaaagccga gcttatgaag aaatcagcaa aaaagtattc tcaaaatttt
      481 ggtctgaatt taaacgtacc ttttcaatcc caagatatgg cgagttacct cgtaagagat
      541 tcgatgatgc tgtttcattt attgaaatgt ggttgccaga aactgcgatc cgtatggaaa
      601 ttgatcaact gaacagacaa cagagacttt ttggtgatga caatgaatag agctgaagcg
      661 ctaagaatag ggacggcaat tgctaattgc tggtggaaat actataaacc aatcatccta
      721 agccaacaac atattgacaa gcaaaaagca tggcaacaaa taaaaaagtg actccgccgg
      781 caagcaaaga gtcacaaaga aaacacatca taaggagatt ttagcatatg gaaaaagaac
      841 tttccactct agatcaatat ttgattgatc ctgattgggg caagccgaaa attgaggaaa
      901 caagtggtcg aaaaatcaga cgaaatcttt tgacgaatga agaactagct tgggatcaag
      961 atgatttagg caaccatgta actatttggg atcatgttta tcttatccat ctatcgaagc
     1021 attcgaataa acctgaatat atttacgtca tcgaagatgg cttgattgat gcgctagaag
     1081 agtacgacag agataacttg attgatatct cttattacgg accaggtaag aaatacattg
     1141 ctgaaatgga ggcagaattt gatgagtgaa atcaaaggga caacgaactt tgaaaaactt
     1201 tttagtcgta agttaaataa aattctcaag aaaaaaggaa attttgatta tttatcttgg
     1261 gctcacgcgt gggagattat gaaaaagaat gatccacagg caacggtaac tattaatgag
     1321 tacaaacact acagggttgt ttctggaact catcaagact ttcttgttga ggaatataaa
     1381 ccttttctta tggatgaaac tgggacttat gtatctgtct cagtaacggt taaaggacac
     1441 acggaaaccg a
//
LOCUS       DANKAZ010000220         1444 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000220 DANKAZ010000000
VERSION     DANKAZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.227"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..1115)
                     /locus_tag="PWY67_002904"
     CDS             complement(<1..1115)
                     /locus_tag="PWY67_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002403348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059093.1"
                     /translation="MVAVYEYLKKNIPGCDDNVNASNRMFFGGYKSDAVIIINEDNML
                     DITELPIDFTMTCSKNCQSDSGREYELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSD
                     MSFNEIYERLLTIDMNKLLKSSKNLRCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDI
                     SENFIGVVMLILKKDSRVETFNWLLENLDLYPTHFRKLKEESKVLFDTLESMKDKLFR
                     TIRNYLEEMRRVYDVLLSDVNFFDDSKESVTCILSGEQLAKKMSSYYGKTYDIDKCNK
                     LLSLMTFIGLIKKLDDEDVPLKMLEYLNRLKEEKALGNFNHKRSNVYRLDVFNATSVI
                     QKLNEDILPLLEKIHFTYQYFSYEWVKICFNEKEAKRV"
BASE COUNT          462 a          278 c          174 g          530 t
ORIGIN      
        1 actcttttag cctctttttc gttaaaacat atcttaaccc attcataaga aaaatattga
       61 tatgtaaaat gaattttttc cagaagtgga aggatatctt cattcaattt ttgaatcaca
      121 gaagttgcat taaaaacgtc taatcgatag acattgcttc tcttatgatt aaagtttcct
      181 aacgctttct cttcctttaa cctatttagg tattcgagca tcttcaaagg aacatcttca
      241 tcatctaatt tttttatcaa tcctataaaa gtcataagtg atagtaactt attacattta
      301 tctatgtcat aagttttacc atagtaagaa ctcatttttt tagctaattg ttcccccgac
      361 aaaatacaag tcacactttc cttactatca tcaaaaaaat taacatcaga aagcaagaca
      421 tcatagacac gtcgcatttc ctctagataa ttccttattg tcctgaaaag tttgtctttc
      481 attgattcaa gagtgtcaaa aagtactttt gattcctctt tcaattttcg aaaatgtgta
      541 ggatacaagt ctaggttctc taatagccaa ttaaatgttt ctacccgact gtctttcttt
      601 aaaattagca tcacaactcc aataaaattt tcactgatat cacaattact ggaatgacaa
      661 taaaaaatgg aatgcccagt tttactggta aaaatactag cagagggatt atgatcgttg
      721 tgaaacagac atcttaagtt tttacttgac ttaagtaatt tattcatatc aatggttaac
      781 aatctttcat aaatctcatt aaaagacatg tcggaggaat caaacaggca gtctttaaac
      841 cactgtttca tttcttcttt gtcgtcattt tttatcaact taacaaaatc ggtttcattg
      901 actagttcat actctctccc tgaatcactt tgacaatttt tagaacatgt catggtaaaa
      961 tcaatcggaa gctcagtaat atctaacatg ttgtcttcat taattattat cactgcatct
     1021 gacttatatc ctccaaaaaa cattctattc gaagcattta cattgtcatc acacccgggg
     1081 atgttctttt tcaagtattc gtaaacagca accattttac catagtcagt aattgcttct
     1141 tttaatagaa agacaatctt gtatttttct agattttcag agtaccgaat acttttttgt
     1201 atcgcaaacg cagaattatg aactaaattg atagataaaa ccttttctac gcttaagtat
     1261 ctactatcat ctttactcaa atctactacc ttccccttct catctttaac agtgttatcg
     1321 acatccagaa taattactct ggtttccaca aaactctcct tgtcattgcc tttattcttt
     1381 gtcttgcttg gaataaatga ttgtgatgaa tttagtatct ctacaaactc atctcttgta
     1441 actt
//
LOCUS       DANKAZ010000221         1417 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000221 DANKAZ010000000
VERSION     DANKAZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.228"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(150..1337)
                     /locus_tag="PWY67_002905"
     CDS             complement(150..1337)
                     /locus_tag="PWY67_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL2059094.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DANKAZ010000222         1410 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000222 DANKAZ010000000
VERSION     DANKAZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.229"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            39..656
                     /locus_tag="PWY67_002906"
     CDS             39..656
                     /locus_tag="PWY67_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059095.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          528 a          182 c          217 g          483 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1261 aaaaaaacta gtagaagaaa gaataatggc ttgataataa agtactacct attaaaggta
     1321 gtactttatt taatttagta taaattaaca tactactata ttttaggttt catattatat
     1381 aaattaataa tatactacca tttgttggta
//
LOCUS       DANKAZ010000223         1375 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000223 DANKAZ010000000
VERSION     DANKAZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.230"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            128..1297
                     /locus_tag="PWY67_002907"
     CDS             128..1297
                     /locus_tag="PWY67_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2059096.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          501 a          206 c          224 g          444 t
ORIGIN      
        1 atatctacac agacaaaatg atagcgtaaa atattttgtg taaatagata tttagggtag
       61 aaaaaaagaa agtccattct gtaaaattaa agttaccaca accgaaattt tataggagga
      121 ctttctcatg cccaatttta ctacagaaat tatggaaaca ctaatcaata aaggtgattt
      181 agatgaatta tttcgatgtc atttagaatt agctgttaat tcattattgc aggctgaatt
      241 aacagctttt cttgattatg aaaaatatga tcgtgctggg ttcaattcag gtaattcccg
      301 taatgggaat tattcacgtt cattcagaac agagtatgga gaattaaatt tggtaattcc
      361 tagagataga aatggtaagt tttctcaaca aactttacct gcttataaaa gaaccaatga
      421 ctctttagaa accactatta ttcagctatt tcaaaaagga attactatgt ctgaaatctc
      481 tgaattgatt gaaaaaatgt acgggcatca ctatacgcca caaaccattt caaatatgac
      541 taaaatagtt tctgaagata ttattgcctt taaagaaagg tccttagaat ccagatactc
      601 tgtcattttt atggatgcta ctcacattcc tttaaaaaga caaactgttt caaaagaagc
      661 agtttatatt gtaattggca ttcgattaga tggaaccaaa gaggttcttg ggtttagtct
      721 tgctccaact gaatctgctt atgtttggaa agagattctt caagacctaa aagaccgtgg
      781 tttaaaagag gttttattag tcgtaactga tggtttaagt ggcattaacg atagtattca
      841 tagtgtttat ccaaatgctc aatttcaaca atgttgtgtc catatatcta gaaatatcgc
      901 tcataaggtt cgtgttagtg atcgacaaga aatctgtagc gatttcaaat tagtttatca
      961 agcaagttca aaagaagaag caaataatca aataaggttt atgatagata aatggaaaaa
     1021 gcagtatcca cgagtggtta aattgctcat gaatcctgct atattaacct tttataattt
     1081 ccctccgtca atcagaagaa ctatttattc aactaatttg attgaaggtt ttaacaaaca
     1141 attaaagaaa tataccaaaa gaaaagagca atttcctaat gaagaatctc tggagagatt
     1201 tctagtatct caattcaaca actataatca aaagtttcta tgccgagttc ataaaggttt
     1261 taaagaaata catgatacat tagaatcaat gttttaactt gatcaacaga atgggctttt
     1321 ccatttacac aaaattattg acgctcccga caaaattatg atcttgcgta aagtt
//
LOCUS       DANKAZ010000224         1355 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000224 DANKAZ010000000
VERSION     DANKAZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.231"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..1355
                     /locus_tag="PWY67_002908"
     rRNA            <1..1355
                     /locus_tag="PWY67_002908"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DANKAZ010000225         1337 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000225 DANKAZ010000000
VERSION     DANKAZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.233"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..522
                     /locus_tag="PWY67_002909"
     CDS             <1..522
                     /locus_tag="PWY67_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2059097.1"
                     /translation="QYRTKGIAYFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTA
                     IFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIY
                     SEKDPTSLQEKIKEQKRKLELLQQLEELERKQNNLNKENKEINEQIPDNNSDIPPELK
                     KGLLDNLQNLFKE"
     gene            532..1029
                     /locus_tag="PWY67_002910"
     CDS             532..1029
                     /locus_tag="PWY67_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2059098.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
BASE COUNT          554 a          231 c          213 g          339 t
ORIGIN      
        1 caatacagaa ccaaaggaat tgcctatttt tcaaaagaag actttcatga attacttgat
       61 atccctaaaa gttatagaga aagtgatata aataaaaaaa ttataaagcc tattcgccaa
      121 gaactaaccg caatcttcaa aggtctgaca atcaaaaaga aatacggaaa aggaagagga
      181 aaacctgtca ttggctatca attcacgttt aaacctgaaa taaaaaaagc agacgacttt
      241 aataaacacg caccaacaaa agaagaacaa aataaagctt tagaactagt taatcaacaa
      301 atttactctg aaaaagaccc cacttcatta caagaaaaaa tcaaagaaca gaaacgaaaa
      361 ttagaacttt tacaacaatt agaagagcta gaaagaaaac aaaataatct gaataaagaa
      421 aataaggaga ttaacgaaca aataccagat aacaattccg atattccccc agaattaaaa
      481 aaaggcttac tagataatct tcaaaactta tttaaagaat aaggggtttt tatgacagat
      541 aaaactatca aagaattagc agatattcta ggtgtttcaa aagataaagt gaagtatcaa
      601 ctgaggaaat tacctagtaa ttttacctac aaaaaaggaa atattatcta cctgaaaaaa
      661 gaagcagtaa caacaattac aaaggtttta tcaggtaatg agccgggtaa cttacctagt
      721 gaattacctg attcattacc taccgattac ctactcaagg agattgaaac taagaataat
      781 catattgaag aaaaagatag gcaaattgaa aaacttcaac tagctttaga aacacaacaa
      841 aaattactcg accagcaaca acaactcacc ctgcaagcca accaacaaat cgagaaactg
      901 caggaacaac tacagttgac ttatacggaa gaatcttcgg aaaataaatc aacgactctt
      961 tctgaagaaa cagaatcgat agaaaaacaa tcaaagacgg agaaaaaatg gtggcaattc
     1021 tggcaataaa accagcaatt taaattaata aaaattgctg gttttattct tctttttgct
     1081 tttgcgagca gagcaacatg ggcccatgtt gcgatatttt ggtggcgccc caatccccgt
     1141 tttttcgttg aatcttactc ggactgcact cgttttacag ctgatttttc tgaattttga
     1201 aagagcataa ggaaacgtgg tatcactttc ttacgaaagg tgctagataa gctgtgccta
     1261 tcacgtttct cagaaaaaaa tttgctatca cttttttgcc attgtggcta ggctatgtat
     1321 agtgttttgg cgaaaaa
//
LOCUS       DANKAZ010000226         1336 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000226 DANKAZ010000000
VERSION     DANKAZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.234"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            86..>1336
                     /locus_tag="PWY67_002911"
     CDS             86..>1336
                     /locus_tag="PWY67_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2059099.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          420 a          263 c          255 g          398 t
ORIGIN      
        1 atcttttttg tataaaaaaa tatagaaaag acaccctgaa atcctttaga attaatttgg
       61 ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag ataccctaaa
      121 tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa aatacaaagg
      181 gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt gttcccattg
      241 tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc ggttgttact
      301 gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat ttaaatgtaa
      361 gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact gtttcattag
      421 tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca tgacggaaat
      481 cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc gattctaccg
      541 cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg aattcaaatc
      601 ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa gccatgcatt
      661 attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt ctcggttttc
      721 attagcgact cgtgaagccg ttcaattgat tgttattgat atgtatgcgc cttatgtttc
      781 gttggtgaaa aagcttttcc cgaatgctca gttgatcatc gatcgtttcc acatcgtcca
      841 acatattggc agaactttct tgaatcatcg agtgaaagaa accacggtac gacttaaagg
      901 aaattcccac tctcaacgag ggttagggaa aaaattgaag cgctattgga aactcctaca
      961 aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt ttaaagatta
     1021 tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact tacgccaagg
     1081 ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt cactatttga
     1141 ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca ctttacgaac
     1201 gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt caaacggacc
     1261 attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg gctttcgcaa
     1321 cttttacaat ttcaaa
//
LOCUS       DANKAZ010000227         1325 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000227 DANKAZ010000000
VERSION     DANKAZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.235"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..286
                     /locus_tag="PWY67_002912"
     CDS             <1..286
                     /locus_tag="PWY67_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2059100.1"
                     /translation="IENEVTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIF
                     DGQCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            291..1238
                     /locus_tag="PWY67_002913"
     CDS             291..1238
                     /locus_tag="PWY67_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL2059101.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          442 a          189 c          272 g          422 t
ORIGIN      
        1 aattgaaaat gaagttacta tagttaataa atcaagatac acatctaggc aacagacacc
       61 tgaagttttt gaaatgaatg cctcaatgta ttcttttaaa cctgaatttc taaaaaaaag
      121 taattatatt tttgatgggc aatgtgggat aataaaaatg agagattatc taattttaga
      181 tattgatagt gaagaagatt ttaaatggtt agaatttttg tttcctaaat ttttagaaga
      241 ttcttcggag cttcaagata tctataatta tttatatcgg aggtaaaagg atgagctgtt
      301 atcttatcac cggtggggct ggcttcattg gttctacctt agccaatcat ttaggagaag
      361 aacatgtggt aatcgtcgtc gatgacttat caatgggaaa agtagaaaat cttgatatga
      421 cgaaaaatat cacttttatt gagggagatg tcgctgagaa atccttgatg gaagagatca
      481 tgaaagctaa taaatttgat tatatctttc atttagcagc agttgctagt gtggcagatt
      541 cagttgcccg accagtagaa acacatcgtg tgaattttga aagtgtatta atgattttag
      601 aattgatccg caagtatcaa cctgatttaa aacggatcgt gtttagttct tcagcggctg
      661 tttatgggga tgaaccgact ttaccaaaaa aagaagagtc cgtgattcgc ccattgacgc
      721 cttatgcggt tgataaattc gctgcagaac aatatgtgct agattattgt catttatatg
      781 atgtacccgg tagcgcagtt cgttttttca atgtctatgg tccaaatcaa aatccgaatt
      841 ctccatacag tggtgtgatt tctattctcg tagatcgcta taagaaacaa atcgtaggag
      901 aagcaacttc atttacatta tttggtgatg gaagccagtc tagagacttt gtttatatag
      961 atgatgtaat ccaagcatta cttttagttg cgaatgagga agctgcactg gggaaacaat
     1021 tcaatgttgg aacgggtaaa gcaaccacac ttttggaatt gattcatgca atcaatgaaa
     1081 ttctcggtgt agaattgaca cttgaataca aagaagagcg ttcgggtgat atccatgact
     1141 ctctagcaga tattactaag atccagtcaa ttggctatca accgaaatat ggtattttaa
     1201 gtggaatgac taattatttg aaaactgaaa tcaattaaaa catactaaga taagattaga
     1261 gggtaaattc aggctctttg tcaataagga ctgatgagtt ttgtaaaatc aaatctggga
     1321 tagaa
//
LOCUS       DANKAZ010000228         1294 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000228 DANKAZ010000000
VERSION     DANKAZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.236"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..1244
                     /locus_tag="PWY67_002914"
     CDS             <1..1244
                     /locus_tag="PWY67_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL2059102.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DANKAZ010000229         1254 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000229 DANKAZ010000000
VERSION     DANKAZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.237"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..559)
                     /locus_tag="PWY67_002915"
     CDS             complement(<1..559)
                     /locus_tag="PWY67_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2059103.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEH"
     gene            910..1209
                     /locus_tag="PWY67_002916"
     CDS             910..1209
                     /locus_tag="PWY67_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2059104.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          457 a          180 c          205 g          412 t
ORIGIN      
        1 gatgttccga gcgtacttca atcatttcaa aggagttgct acttaaatcg atatttttta
       61 aatcagtatc aaaaaagcta gcattttcta aatcattagt agaaaacatt attttagtga
      121 gggaacagtc tacaaaatct gcgcttgata agacagagtg ctcaaaggta ctttctttta
      181 tgtttgaacc agataaattt acatagtcca ttttacaatt ctcgaataac gtgttcccaa
      241 tataactttc catcaattta gtacctgtta atttacaatt gacaaatttc acgcgatgca
      301 acatgctttt tgtcaatata cagcctgaaa aatcacagtt ttcgaatata cagtcggtaa
      361 tctccattct ttgaaaatca ttggaagcaa aaaatgcata cttgaaaata gatttgtaaa
      421 taattggatg gcagattatt tggttttcga tgacctcttt ttgtgaaagg atattataca
      481 gttcaaaatc ttcgttttca tagatatgaa aaaaggatcc tttattcaag cttgcttcat
      541 taactttagg aaaaatcata tttaattacc tccattttca taaagtcata ttaacaaaag
      601 ctaaaccaac ttacattagc agaaaatgag ataggttttc gcctttttcg ataacgatag
      661 tgtcgctatc tcttgaaacg ttcttagaat tgccctgaga gacacaaatt aggcgtaatt
      721 ctaataaaaa tcttagaata aaaattttta ctgatttaat ttagaatgtt tttaaaataa
      781 aaatcgttgc cataacagtg tattgaaagg ttttaacaat ccagtaatga tttaaaaaaa
      841 tctagatgta gcgtatactt tgtttataga atgtagtggg aagaaatata tttttgggag
      901 gaaatcgtga tgtctaaaag tgaagaaata gaaaacatgg tactaaaatt atatgatctc
      961 gttttaaatc cagagatcaa agaaaaggaa cgtatacttt tgatagacgc aaagacaggc
     1021 ttagaaaaag gtcagtatta tcctaaagtc ataaataatt tggaacgtag tttacgtccg
     1081 ttagcaatca ggggagaatt gtctaaacca gtatcaccgt tttatatgga gatatcaaca
     1141 ataggaaaat ttgaaaaaga attaggtcga ggaatggctt cggcaccgat tacttttggg
     1201 catttataaa aacgagttat tcatctcaat gaattaaaaa gtatagtcaa tctc
//
LOCUS       DANKAZ010000230         1251 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000230 DANKAZ010000000
VERSION     DANKAZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.238"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(62..280)
                     /locus_tag="PWY67_002917"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="PWY67_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="PWY67_002918"
     CDS             complement(546..740)
                     /locus_tag="PWY67_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059105.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="PWY67_002919"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="PWY67_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          443 a          212 c          170 g          426 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta c
//
LOCUS       DANKAZ010000231         1246 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000231 DANKAZ010000000
VERSION     DANKAZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.239"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            82..>1246
                     /locus_tag="PWY67_002920"
     CDS             82..>1246
                     /locus_tag="PWY67_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059106.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPD"
BASE COUNT          404 a          128 c          220 g          494 t
ORIGIN      
        1 aatgttcttc acttaaagcg caaaaaatat cagtttatta aactcgtata tagaacaaat
       61 atatgtagac ggggcgaaac gatgagtgta aaaaaaatgc tatttatggt aagtacaata
      121 attgtattaa catcccgatt ttggggaatt aatactagtt tatctatgcg atattttatt
      181 atggcaatat gggatttata ttttatgtta agctattttt cttatcgtga gaaaacatat
      241 aaaaaatacg ttatcaagaa taacttttgg ttgactataa tgccgtttgt gatattttct
      301 atctatactc taataatatg gggaattggt tcaaatgcag aatttgaaaa ttttataaag
      361 ttatgttcca cgttgcttta tttaattctt gcatatggtt acgcatgtac agcattttac
      421 ttatttaagg cagaaggaat tgattttatt ttttggtcag gtgttatttc atatatgagt
      481 ggttcagttt tttatttaat tgtatcttat ggtttaaatg gtttaatttc ttatataaaa
      541 agtcttataa caggtgaaac aaatactgca aattttgcta tggaggtaca tgatttaact
      601 tttgcgatgg gggttttttt cctttattac gtattttttg aaaataaaaa tacaaaaaaa
      661 cataagataa aaattatcgt atctttaatt ttaattttca tgggattgaa gagaattgaa
      721 ctgttagctt taagtggtgc aattctagtg tattatgttt tattaaagtg gggaaaaaag
      781 attcaaatcc gtgcattagt gtgtactatt tgtgcaactg ttatttcgtt agtgtttatt
      841 tacattatag atacaggaat tttggcattg cttatgtcaa atttgggcat tacagatgac
      901 ggtgcgaggc tttcatttta tctatattca tctaaatttt atgaacttgg tttaggatat
      961 gtaggaacag gatggacatg gttttcaaaa tactttttta atctttattt atcgcacttt
     1021 aggttaaatg gtggaagaat tgcagcaagc ctacacagtg atatattaac tatttatata
     1081 gaagttggat gtatagtttt tattatatgg atgttttggt tattttttgt gaaaccgata
     1141 agactaacta aaaaatttgg attgaaaaca ggggagtgtg tattattatt aactatatat
     1201 atgtttatat tatatttgac ggataatacg ctcacttatc cagaca
//
LOCUS       DANKAZ010000232         1201 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000232 DANKAZ010000000
VERSION     DANKAZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.240"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..>1201
                     /locus_tag="PWY67_002921"
     CDS             <1..>1201
                     /locus_tag="PWY67_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL2059107.1"
                     /translation="QKKKTLVVDAVLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVV
                     IDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMR
                     IRIFLIN"
BASE COUNT          397 a          238 c          231 g          335 t
ORIGIN      
        1 ttcagaagaa aaagacgttg gtcgtcgatg ctgttctctc gcctactcct cgtgcttgta
       61 gaagttgtgg ttctactgtg gtagatggaa acgggaaagc aattatagtg aaaaatggaa
      121 aaaaggaaac gattgtccgt tttgaacaat acaatcatat gcctttggtt atgcgcctaa
      181 aaaagcaacg ctatacctgt aaaaactgtc gcacccattg gacggctcaa agttattttg
      241 tccaacccag acattcaatc gcaaatcatg ttagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa gctgtcaggt atctttgacc accgttattc
      361 gtacattgaa agagtttaaa agctatttac caaagcaatc taagaagatc ctccccagag
      421 tattgatggt tgacgaattt cgttcgcatg cttccataga agataagatg agctttattt
      481 gcgcggatgg cgaaacagga aaattaatag atgttttgcc tacgcgtaaa ttacctcgat
      541 taacaagcta tttcttaggt tgtaccaatc cagaagaagt agaattcttg gtgacagaca
      601 tgaacgccgc ctacttccag ctcaccaaac gtgttctgcc aaatgcgaaa gtcgtgattg
      661 atcggtttca tattgtcaaa cacatgaatc aagcgttcaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagcc ggacagaaaa gccaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 tccgagcacc aaaataccca tttcttactg aagcaatgat gattgatcga ttgcttgagt
      901 tttctccgcc cctaaaggag gcgtatccct tttttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgaca
     1021 gctttcggga aaagcttcaa aaccttctga cctatgaaga aggcatcacc aacgcaatga
     1081 tctatcctta ttccaatgga aaaatagaag cgaagaatac ccacataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt ttgatcaatc
     1201 a
//
LOCUS       DANKAZ010000233         1195 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000233 DANKAZ010000000
VERSION     DANKAZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.242"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..1076
                     /locus_tag="PWY67_002922"
     CDS             <1..1076
                     /locus_tag="PWY67_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2059108.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DANKAZ010000234         1186 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000234 DANKAZ010000000
VERSION     DANKAZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.243"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(485..850)
                     /locus_tag="PWY67_002923"
     CDS             complement(485..850)
                     /locus_tag="PWY67_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059109.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(926..1171)
                     /locus_tag="PWY67_002924"
     CDS             complement(926..1171)
                     /locus_tag="PWY67_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2059110.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
BASE COUNT          397 a          215 c          230 g          344 t
ORIGIN      
        1 actattttta ctatcaatat taacgctagc aatggcaggt tgttctgact ctggcttagc
       61 aactgaactg atcacaaaat caactgaagc cacgacagag gagttaaaat gtggaaacga
      121 tcacagtgac tacagaggct taaatcgacg atgcatggga atattaggca gaagcaattg
      181 taagcatcga tgtagtaatc gaagaaggtg tagaagaagc aacagaaaaa tcaaaagaaa
      241 gatcaaaaga agcaatctgt gctgtgcatc gtatacagac gtcgaagatg cggtactttt
      301 aaaccacgtc aggagatgtt catttatgtc tagggaagtt ggcgatgaac caaaactaga
      361 gtacgctttt agttttgcac gtgaggatga agataaccct aaaacttacg acattttagc
      421 ttgatctaag atggttaaag ctttttaatg aagtaagagg agatagaagg cttttctagt
      481 tatattaggt aagataaatt cctttttgct ccaaatttgc taaacattct gctagataaa
      541 cttcatcgtg ttccggataa atcggcacgt tgcgctctac agtaggtaaa tcaaaaaatg
      601 cttcgcatga tttgccacga tacataggat ttttccactc tggtgctggg ctgtaaccac
      661 gtttttccat ttcatccatg acaagctgat gatattggaa taatctataa ggagaatagt
      721 gaaaaacata atccacagta gcatgtttct ttccccagcc atttcctctt aatgcacagc
      781 attcccgatg ttgtcctaaa agctgttgac gaggaagctt tgaaatcaat ttttcatgcc
      841 ataatcgcat agaaatgtct cctttatata tagattgtta accttagacg ttaaatgaaa
      901 aaaccagcac caaggctgga aaaaatcact tcagtttgct tgctgtttct gagcgaccaa
      961 ttaggtaatc aacggaaata tcgtaaaagt cagcgatata gattaattca atgatgcttg
     1021 gaatttcttc tccttcttcg tacttacgat atttgaataa aggaatactt aacgcgctag
     1081 aaacagctac ttgagataaa gatcggccag ttcttaagaa attcagacga caacgtaaag
     1141 tatccagtaa aatagcttct tcgatactca tttaatcacc tttcat
//
LOCUS       DANKAZ010000235         1183 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000235 DANKAZ010000000
VERSION     DANKAZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.244"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..153
                     /locus_tag="PWY67_002925"
     CDS             <1..153
                     /locus_tag="PWY67_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2059111.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="PWY67_002926"
     CDS             159..575
                     /locus_tag="PWY67_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059112.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="PWY67_002927"
     CDS             681..944
                     /locus_tag="PWY67_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2059113.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1183
                     /locus_tag="PWY67_002928"
     CDS             944..>1183
                     /locus_tag="PWY67_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2059114.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVLLRSLKIKR"
BASE COUNT          448 a          154 c          239 g          342 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggttctgt tgcgaagttt gaaaatcaaa cgc
//
LOCUS       DANKAZ010000236         1181 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000236 DANKAZ010000000
VERSION     DANKAZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.245"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..>1181
                     /locus_tag="PWY67_002929"
     CDS             <1..>1181
                     /locus_tag="PWY67_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2059115.1"
                     /translation="EEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTEIVDSKVTIEK
                     FAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFGVYFGVFNGFS
                     MPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGNQPIGKRFFSD
                     EYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELDGAFGLAEAHF
                     HDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNWIFDFEMTSFT
                     SKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFLLVGICPVSYF
                     EAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSDCLLEKFNKEQ
                     I"
BASE COUNT          408 a          171 c          216 g          386 t
ORIGIN      
        1 gaagaagcag agatccatgt cgaacgtctg aatttgacgg atatcaaaaa tttaagaaga
       61 atctactttc aacgatcaaa aattgctcga ctgttaacgg agatcgtgga ttcaaaagta
      121 acgatcgaaa aatttgcaga gaatcatttt ttaagcatat caagagccta tgtaaaaaga
      181 agagaattag tctcttttct tgaacagcat caaataaagt taaagaaaaa catattagtt
      241 ggggaagaaa taaacatacg gaacgtatta tttggggtat atttcggggt ctttaatggt
      301 ttttctatgc catttagaga aagcgtcatg aatgaagcaa agagtatcgt gcaatatttt
      361 gaatatctgt tcaatttaaa actgtctgca acaaaacgaa tcaaattcaa tttgctaacc
      421 actattagtt tgttgagggc ctatgggaat caaccaatcg gaaaacgttt cttttcagat
      481 gaatacttta agagctcaga aggaatgaag atcatcaagg atttatcaca tatttctttt
      541 cttcatatcc ctaaagagca gatcattgat gagatttctt atatcatgtt gtttttgatc
      601 acggaagaag aatttgaatt agatggcgca tttggacttg cggaagctca ttttcatgat
      661 atccacatga ttagtcaaaa aatgtcagtg gaattgattg atcaattacc attttcgatt
      721 gaaatctctg aagaagaaag actatatata cgaaaagaac ttcgtatggg tatgatgcat
      781 gtcaatcgaa aaaattggat attcgacttt gagatgacta gctttacgtc taaaaagcag
      841 ctacagtttt tcttcgagac gtatccaata ttcagtacag caatccaaaa ggtcatacaa
      901 gagaataaac gctatttctt gggaaaagat gaacgtttca tgattcgctt attttatgat
      961 tatctctttt tattggtcgg tatttgtccg gtatcatatt ttgaagcacc gattcatgtc
     1021 tgtattgatt tttcgcaagg atctgcttat accgattaca ttatttctca agtgaaagga
     1081 tttaaaaatt acaatattgt gattgagagt cgaatcactt ctcgtacgaa tatttattta
     1141 tctgattgtt tacttgaaaa atttaataaa gaacagataa t
//
LOCUS       DANKAZ010000237         1160 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000237 DANKAZ010000000
VERSION     DANKAZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.246"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            14..1141
                     /locus_tag="PWY67_002930"
     CDS             14..1141
                     /locus_tag="PWY67_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2059116.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          419 a          200 c          224 g          317 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa
//
LOCUS       DANKAZ010000238         1158 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000238 DANKAZ010000000
VERSION     DANKAZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.247"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="PWY67_002931"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="PWY67_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HDL2059117.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          341 a          233 c          169 g          415 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaatgggg gttctgttgc aaagttttaa
     1141 atctactatc aaataagg
//
LOCUS       DANKAZ010000239         1150 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000239 DANKAZ010000000
VERSION     DANKAZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.248"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            127..912
                     /locus_tag="PWY67_002932"
     CDS             127..912
                     /locus_tag="PWY67_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HDL2059118.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          447 a          176 c          181 g          346 t
ORIGIN      
        1 aaaaagatac actcctatct cttatttaaa gttgctatat accctcttat tttagcatac
       61 tcccttttag aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg
      121 acgtatatgc ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca
      181 gaaccggcat ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat
      241 aaaaatttaa aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt
      301 atatcagtac ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg
      361 tgtaataaat acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt
      421 ggcgaaaaag atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa
      481 aaagtattga atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac
      541 ggaagtattt tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc
      601 gccggctctg tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta
      661 caagtgaaaa ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca
      721 acaattagaa aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct
      781 atccccaatt ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag
      841 ttaactgaaa atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca
      901 ctcgatgact aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa
      961 caaacaaaaa aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt
     1021 ttggatttac gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc
     1081 aattactaaa cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt
     1141 agtttaaaag
//
LOCUS       DANKAZ010000240         1144 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000240 DANKAZ010000000
VERSION     DANKAZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.249"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(19..924)
                     /gene="xerC"
                     /locus_tag="PWY67_002933"
     CDS             complement(19..924)
                     /gene="xerC"
                     /locus_tag="PWY67_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL2059119.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          380 t
ORIGIN      
        1 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
       61 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
      121 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
      181 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
      241 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
      301 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
      361 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
      421 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
      481 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
      541 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
      601 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
      661 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
      721 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
      781 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
      841 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
      901 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
      961 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     1021 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     1081 aagggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc ccattttcat
     1141 ttta
//
LOCUS       DANKAZ010000241         1074 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000241 DANKAZ010000000
VERSION     DANKAZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.251"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..276)
                     /locus_tag="PWY67_002934"
     CDS             complement(<1..276)
                     /locus_tag="PWY67_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2059120.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
     gene            838..>1074
                     /locus_tag="PWY67_002935"
     rRNA            838..>1074
                     /locus_tag="PWY67_002935"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          351 a          194 c          205 g          324 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgatatatta aacaacgttg tcgcgcttca ttgaaacatt ttatgaaaat
      421 tccaaaaaag agttgacaca tgtttttaga cgtgataaga taactaagtc gttaaaaaga
      481 accacttgcg aagcttgatt ctatcgaaaa aaaacttcaa aaaagttctt gacaaataac
      541 aaacgataag ttatgataaa tgagttgctg aaagatttcg aaaaacgaaa tcgaaaaaaa
      601 cttttaaaaa agagttgaca atcgcttgtc tctctgatat gatataaaag ttgctacggc
      661 agccaaaaac gaatcgggaa acactcccga tgaagtagac ctttgaaaac tgaacaaagt
      721 aagacaaacc aaatgtgtag ggcgtcttga ttcaattcaa gacaataaac atttttaaca
      781 agcaagcaat atgctagcaa acaaattgag cttaacaatc gcaagattgt tcgaactttt
      841 tatgagagtt tgatcctggc tcaggacgaa cgctggcggc gtgcctaata catgcaagtc
      901 gaacgcttct ttttccaccg gagcttgctc caccggaaaa agaggagtgg cgaacgggtg
      961 agtaacacgt gggtaacctg cccatcagaa ggggataaca cttggaaaca ggtgctaata
     1021 ccgtataaca atcgaaaccg catggttttg atttgaaagg cgctttcggg tgtc
//
LOCUS       DANKAZ010000242         1074 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000242 DANKAZ010000000
VERSION     DANKAZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.252"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(37..846)
                     /locus_tag="PWY67_002936"
     CDS             complement(37..846)
                     /locus_tag="PWY67_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2059121.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          323 a          205 c          189 g          357 t
ORIGIN      
        1 tgtcacaacc tcttttttgt ggaatctaat tctctattat agtttaagta gtttttccag
       61 ataaacagct aaaccgtctt cctgattgct caaaggagtg acatcatttg caatgccttt
      121 gagttggtca gtaccgttag ccatagctac tccccaacct gcataatcca acaattccaa
      181 atcattatgc tcgtcaccaa aggcaatgac gtctttttga tcgatagaca ggtagttagc
      241 aatctcttgc acgccttttg ctttttggat tccttttgaa acaatctcta agatggcagt
      301 aggtccaccc catgtccgaa catcgacata actgccaaat tgacgtgtta attcagcaga
      361 tacagctcct gcaaaacgtt tgtctgttcg gacaagcaaa gatgtaggat ttgtcgtcaa
      421 atttttagga gaaagcaaat tcttctctcc tggacgagaa gaagcaaaaa ttttttcatc
      481 aaagaaatca aaactgtcaa taaaaaaagt atcacgattt tcagcagcga taaaatctaa
      541 atttagctgc tttttttgcg ataagatttc aaaagcgatt tcacgattga tcaacgtttc
      601 tctttcacca tcccagtgct gattagggat gtgtactaat gcgccgttaa aattaaccat
      661 cggtgttgtt aattctaatt gttggtaaaa ctgatggctc attcggaatg ggcgtcctgt
      721 agcaatactg acatgatgac cggcattgat tgcttttttt agagtctctt ttgttttaga
      781 tgtaatcatc gaatcttgat tgagggtagt tccgtccaga tcgatggcaa ttaatttttt
      841 attcaataag ggtccttcct tcctcaacta gcatttgttt tagtctagca tattttaggt
      901 aattaacaag agcaatcagc tattcctaac aaaaactaca gttatggcta tataaatagc
      961 taaacataag ctaaacacga acgttaagta ttattttcgc tttttttgtt aaaaaaacgt
     1021 tgaaaagcga atgaaacgtc tatataattt gaaattgttc gatggatttg ccct
//
LOCUS       DANKAZ010000243         1063 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000243 DANKAZ010000000
VERSION     DANKAZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.253"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(38..994)
                     /locus_tag="PWY67_002937"
     CDS             complement(38..994)
                     /locus_tag="PWY67_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059122.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caa
//
LOCUS       DANKAZ010000244         1051 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000244 DANKAZ010000000
VERSION     DANKAZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.254"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="PWY67_002938"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="PWY67_002938"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2059123.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DANKAZ010000245         1018 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000245 DANKAZ010000000
VERSION     DANKAZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.256"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..489)
                     /locus_tag="PWY67_002939"
     CDS             complement(<1..489)
                     /locus_tag="PWY67_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2059124.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSM"
     gene            complement(489..>1018)
                     /locus_tag="PWY67_002940"
     CDS             complement(489..>1018)
                     /locus_tag="PWY67_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2059125.1"
                     /translation="WHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVM
                     TQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPD
                     SLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFE
                     KDKYIINLINDIEVN"
BASE COUNT          352 a          176 c          112 g          378 t
ORIGIN      
        1 catagattta aaggcagaat aattatttct ataatcagta ccttttctcc aatcaggaag
       61 ataagtagaa caaatagctc ttacagaata tactttttta ctttgaatac tattacgaac
      121 aacttccatg atatttttat acctcagtgg agcagcaaca taaatatttt tgtaatcata
      181 attatattga gtaagatcaa tactctcaga aaaagcaggt ttttctaaaa atatgtaatc
      241 aactttttca acaattacat ttagtgtttc cagatgctta aaagtcggat ttgtaataaa
      301 agctatatca taatgtgtat ccaaatcgtc tctattttga atatacgtta tttcttcaaa
      361 ctcttttttt ccactgctac tataagctgt tatatcaaaa ttaatagaat tcttttttcc
      421 atagcttatt agatttttta gatgtctctt tccaattgac cctaacccaa aaaaaattac
      481 tttcatcatt aatttacctc aatgtcgtta attaagttaa taatatactt atctttttca
      541 aaagtatatt ggggttccat tttcttttcc aaaagcgtga aaaattcttt aatctcctct
      601 aaatatgcat cttcaataat agtatgtgaa taattattat catgcataac ctcatcgtac
      661 aattgaatat ctgtcatact agaagtttct tcattccatg aaaatagaga atccgggtta
      721 ccttcccatt ctattaaaat cttttcacca attatagtta aatttctttt agctactctt
      781 gaaacaacat ctacacttaa agttccaact ataccagttt catgctcaat aataagagtg
      841 aaactgtccg aataatcaat atcaagattg gataatttct gggtcattac agtaaacgat
      901 ttaactttcc caaatgtatc tataatccaa ggtaattcaa tagcgaatat ttctctacac
      961 ccattagttt ttttgtttga aacaaaaaaa ttgcgataat cttcccaagg atgccagt
//
LOCUS       DANKAZ010000246         1012 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000246 DANKAZ010000000
VERSION     DANKAZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.257"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            337..576
                     /locus_tag="PWY67_002941"
     CDS             337..576
                     /locus_tag="PWY67_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059126.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          345 a          178 c          148 g          341 t
ORIGIN      
        1 tagaagtaat aatattgcta ttattaagat aaaattactg taaaaatcca aaagctatcc
       61 tcctagcaac tatttaaata cccaaaaaaa ttacctctag ataatatcat acctacgttt
      121 tcacttataa attttttcat aaaaaaagcg tttagaggca taatcgttaa tacagatggt
      181 aaccaatgta tctggtttga actgggatat caccctctcc atatatgtat tactaagact
      241 aatagatact ataaaagact tgatagtttt gcattgattt tgcctaatac tgagcatagg
      301 tgtatgctaa acgatactat aaggagtgtg tacataatga aaagaattct gtttatcagt
      361 ttatcaaatt tattgttact tactgcttgt gggacttctg catcaaagaa cataccttca
      421 gatacgcaaa tttcgaatag tatcgattca acaatttcta caaatgagaa aaattctccg
      481 gtagaaactt ccgatataaa agtttctacc tctgcaaaaa acagggtaaa gaaactgtca
      541 gcacgtctcc tttatttcaa acaaactctg aattagcttc ttcatcttct tcagcaaata
      601 atgatgatag tttcgcgttt actcctaact aaatgcaacc tttacaaatg ctttacgata
      661 acaagcctga gctaaatgat cctgatatca gttttgcatt ttttgaaatg attggatcgg
      721 atgatctttt taaaactact agtttgtcta tccgaaaata ggacagttct ggcagtatag
      781 gtttttatcg agtttctcca ccaggcctcg ttacagagac agattctcta ggcgaacctt
      841 ttgaataaat caaaaatcat gaacaatcac tgccaactaa ataaagtgcc ttcaaaagct
      901 tggtcttctc gcttttgaag gcactttatt taaatcttta tgaaactaaa gtatgttcat
      961 atttgttgct atagttatat agatattctg ttgtttccaa ctcataaacg tg
//
LOCUS       DANKAZ010000247          995 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000247 DANKAZ010000000
VERSION     DANKAZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.258"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(26..913)
                     /locus_tag="PWY67_002942"
     CDS             complement(26..913)
                     /locus_tag="PWY67_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2059127.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DANKAZ010000248          994 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000248 DANKAZ010000000
VERSION     DANKAZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.259"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..>994)
                     /locus_tag="PWY67_002943"
     rRNA            complement(<1..>994)
                     /locus_tag="PWY67_002943"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DANKAZ010000249          989 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000249 DANKAZ010000000
VERSION     DANKAZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.260"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..>989
                     /locus_tag="PWY67_002944"
     CDS             <1..>989
                     /locus_tag="PWY67_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2059128.1"
                     /translation="EKLKEIEEKIIQYDGLAKLKDAKVVSGEQRINREDLSDEFKNVV
                     SLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELL
                     DIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEI
                     ANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMT
                     YSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISS
                     LFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIV"
BASE COUNT          411 a           83 c          164 g          331 t
ORIGIN      
        1 ttgaaaaatt aaaagaaatt gaagaaaaaa taattcaata tgatggatta gcaaaactaa
       61 aagatgctaa agtagttagc ggagaacaaa gaataaatag agaagattta tctgacgaat
      121 ttaagaatgt tgtttcactc gaagctacaa ataatactaa aagaaatatt ttatttagta
      181 gtggagtatt tacaattaaa gaaggtaaaa atatagaaga aaatgataag aattcaatta
      241 ttgttcatga agaatttgct aaacaaaaca atctaaaatt aggtgatgaa gttgatcttg
      301 aattactaga tattgaaaaa agtggaaaaa taaaaagtca taaatttaaa attataggga
      361 tcttttctgg taaaaaacag gaaacatata caggattatc ttctgatttt agtgaaaata
      421 tggtgtttgt agattattca acaagccaag agatattaaa taattcagaa aataatgaaa
      481 ttgcaaataa aattttaatg tattctggta gtgcagaatc tacagactta gccttaaaca
      541 aattgaaaga gcttaaaatt gatgagtcaa agtattttgt tgaaaaagat tctaatgcgt
      601 ttgaagagtc tttagagtca gtgagtggaa taaaatatat aataaaagta atgacttatt
      661 ctattatgtt aggtgggatg gttgttcttt cattaatctt gattctatgg ttaagggaaa
      721 gaatttatga aataggtata ttcttatcta ttggaacatc taagatacaa attataatgc
      781 aatttatatt cgagttaata ttcatatcga taccaagtat aatatcttcc ttatttttag
      841 gaaatgtatt actaaaagta attgtagatg gattcattaa ctcagaggat tcaatgattt
      901 ccggtggaag tttaataaat aatagtagtt ttatgtcaaa tataacaaca cttggacaaa
      961 gttatttaat attaataagt attattgtt
//
LOCUS       DANKAZ010000250          988 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000250 DANKAZ010000000
VERSION     DANKAZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.261"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            94..342
                     /locus_tag="PWY67_002945"
     CDS             94..342
                     /locus_tag="PWY67_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059129.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            373..552
                     /locus_tag="PWY67_002946"
     CDS             373..552
                     /locus_tag="PWY67_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059130.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(595..>988)
                     /locus_tag="PWY67_002947"
     CDS             complement(595..>988)
                     /locus_tag="PWY67_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059131.1"
                     /translation="ELNASYFAADMIYTKVIISNYSTEPAMLVNLELSSANLNRRWSA
                     TPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSAISCYLAFEVGKINFNKLLNHQTKMIF
                     TLNRTQIHKIVDIKNTNFPIEKLVKELN"
BASE COUNT          348 a          140 c          200 g          300 t
ORIGIN      
        1 agatattcta aacggcaatc gtgatggaaa gaaagcacaa cagcatatcg aaacagtgaa
       61 aaaaatattg gacatccgat aggggggtag gcgatgacaa aactaaaaaa acaagatttt
      121 gtaaaaaaat acaattattc tccatctact tatcaacgtc gaatgtcgga actaaaaaat
      181 acagcaattt tctcagcggc gtatgaacgg gtcacaggac aagaagtttg gatcaataca
      241 gaattatacg ataaattttt gtctttcaaa tcctataaca ggttacgcac aagaaaggta
      301 acacctaaag aatttatcga gaagcattta gttgatttat aaaaaacaga acattttgag
      361 aggtgaaggt taatgggtaa attcaacaga gcattggtat tcagcgcacc gctaatcatc
      421 tacgctttag gactttgggg aagcaggcaa gcgttgatag gaacgattgt ttacatggtt
      481 tggattttta tggggcttga tgaagctgaa gctgagtaca gagcgaaaaa gccaaccgag
      541 ggggctgact aaaaatgaat agaaaagaga aactagaatg tatattacta ttactcagtt
      601 taattctttc actaatttct ctattgggaa gtttgtattt ttgatatcaa caattttgtg
      661 gatttgggtt ctatttagag taaatatcat ttttgtttgg tgattaagga gtttattaaa
      721 attaattttc cctacttcaa atgcaaggta acaagatata gcacttttag gaggaatatt
      781 tagcggtaca gcttcagagt atattctatt atcatcgact gatcctcctt tagcaattaa
      841 tttcttaaat ggagtagctg accatctgcg gtttaagtta gcggatgaaa gttctaaatt
      901 aactaacata gctggttctg ttgaatagtt agaaatgatt acttttgtat aaatcatatc
      961 tgcagcgaag taagaagcat taagttca
//
LOCUS       DANKAZ010000251          988 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000251 DANKAZ010000000
VERSION     DANKAZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.262"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..704
                     /locus_tag="PWY67_002948"
     CDS             <1..704
                     /locus_tag="PWY67_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2059132.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          335 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataa
//
LOCUS       DANKAZ010000252          972 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000252 DANKAZ010000000
VERSION     DANKAZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.263"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..848
                     /locus_tag="PWY67_002949"
     CDS             <1..848
                     /locus_tag="PWY67_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2059133.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          314 a          191 c          198 g          269 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tg
//
LOCUS       DANKAZ010000253          959 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000253 DANKAZ010000000
VERSION     DANKAZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.264"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(107..511)
                     /locus_tag="PWY67_002950"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="PWY67_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="PWY67_002951"
     CDS             complement(570..>959)
                     /locus_tag="PWY67_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2059134.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DANKAZ010000254          958 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000254 DANKAZ010000000
VERSION     DANKAZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.265"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..400)
                     /locus_tag="PWY67_002952"
     CDS             complement(<1..400)
                     /locus_tag="PWY67_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HDL2059135.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFII"
     gene            complement(393..>958)
                     /locus_tag="PWY67_002953"
     CDS             complement(393..>958)
                     /locus_tag="PWY67_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2059136.1"
                     /translation="YKHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIR
                     HHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSF
                     KNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFV
                     RKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          343 a          146 c          108 g          361 t
ORIGIN      
        1 tgataatgaa aacattatta aaatacacca tttttttctt tttttcctct tcactttgtg
       61 aaaagtctgc aggaaaaatt actatcaaag taatcagcaa atatccaaaa ttataaatag
      121 atggaattaa aaaattttct ctgttaatga ttatagttaa gataaaacta atcattgctc
      181 caatcagtag ccaagaaaaa cgcattttaa aaaaattttt ctttataaaa acctcataca
      241 aaactataat tccaccccaa gcaaaagcta ttttcatcgg gatattagtt acattaaaag
      301 tactaggcat aaaataaagc agacaaatga ttaaatttat tactcgaaac gcatctcgac
      361 ttaataaact cttctctaaa acaggtacta ttctattcat ctatatttcc aactttcaaa
      421 atttctttat caattctatt taatagttta tcagagtaat ccgatttaac ttttcttaca
      481 aataattttt cactttcttt tatttgatct atatcccttt catcaaacca tttgttcatg
      541 gaaggatcaa taatatgaat atttgctaat ttcgaagttt gtaaagtagt ttcttcaata
      601 tttttcaatt caagtggtaa atctggtcct ccaataacat ttttattttt gaacgaggaa
      661 ttaaaaaaaa gtgtatgaaa aaatttttca tctggggcaa acatacctat tttaaaattc
      721 aggtagtctt gttgattttg ttcatacatt tgtattaatt ctgttaggca ttctttcgac
      781 aaagcccacc attgagaacc atgatacaaa tcaaatttat ggtgtctaat ttttggggga
      841 agtttaatca tcctcagaca acgattaatt atagctctaa aaattttaaa tgcagttgtg
      901 ttattcctat taattctagg ataatcatgt ttttgatata catctatatg tttataat
//
LOCUS       DANKAZ010000255          940 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000255 DANKAZ010000000
VERSION     DANKAZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.266"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            307..940
                     /locus_tag="PWY67_002954"
                     /pseudo
     CDS             307..940
                     /locus_tag="PWY67_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          314 a          178 c          174 g          274 t
ORIGIN      
        1 ccttcaatta tctaaatctc gaaaatggtt agatgtaatc atcaggaaaa acaatcttaa
       61 acgaattact attcatggtc ttagacacac acatgctagc ttgcttttag caagctagtg
      121 caaatattaa agatgtgcaa gagcgtttag gccactcttc tattcaaatc attatggatc
      181 tatatattca tattacagac aaaagaaaag aagaaaccgt agcgcaattc gcaaaatata
      241 ggctctataa tttatgggac tgatgaatca aaattgattc ttcagtccca ttgttatttt
      301 aggtatatga ttcgtacatt gaaagagttg aaaaactatc ttccgaagcc gtcaaaaagg
      361 accctgccaa aagtattgat ggtggatgaa tttcgttcgc acgcttcaat tgtggataaa
      421 atgagcttta tttgtgcgga tggcgagact ggaaaactca ttgatatttt gcctacacga
      481 aaattgtctc gattaacaca ctattttagt cgctgtgcaa atcctgaagc ggtggaatat
      541 ttagtgacag atatgaacgc cgcctacttc caactgacaa aacgtgtctt accaaatgcg
      601 aaagtcgtca ttgattgttt tcatatcatc aaacatttga atcaggcgtt caatgatttt
      661 cgtgtacaag aaatgaaaag attacaccag ttagggcaac gacgggaagc cgagaaagtg
      721 aaaaagaact ggcgctttct tctaaaaaac cgtcaaaaaa tcaatcacta tgattacaaa
      781 acatggcgca gttttcgtgc acctaaatac ccgctactca ctgaagcaat gatgattgat
      841 cgattgctgg cattttcaac gcctttaaag gacgcctata cttttttcca tgagctgacc
      901 gaagccttta gagacaagga atcagactta ttttttacct
//
LOCUS       DANKAZ010000256          913 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000256 DANKAZ010000000
VERSION     DANKAZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.267"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            84..>913
                     /locus_tag="PWY67_002955"
     CDS             84..>913
                     /locus_tag="PWY67_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HDL2059137.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          319 a          134 c          166 g          294 t
ORIGIN      
        1 atattttgtg taaatagaaa aaaggaagtc ccttctgtag aatagagtta ccacaacaca
       61 ttcacagaaa agaggacttc catatgaacg attttactac agaaattatg gaaatactaa
      121 tcaataaagg tgatttggat gatttgtttc gtcgtcacct agaattggct attaacgctt
      181 tattacaagc tgaattaacg gtatttcttg actatgaaaa gtatgataga gctggattta
      241 attcaggtaa ttcccgtaat gggaattatt cgcgctcttt taaaacagag tacggtgaat
      301 tgaatttagt aatacctaga gataggaatg gagacttttc tcaacaaact ttacctgctt
      361 ataaaagaac caatgactct ttagaaacca ctattattca gctagttcaa aaaggaatta
      421 ctatgtccga aatctctgat ctaatcgaaa aaatgtatgg tcattattac aaaccacaaa
      481 ctatttcaaa tatgagtaaa attgtgtctg aagatgtttc ggcatttaaa gaaagaactt
      541 tagaagctaa atactcagtc atttttatgg atgctactca tattccctta aaaagacaaa
      601 ccgtatcaaa agaagctgtc tatatagtaa ttggtatcag gttggatgga actaaagagg
      661 ttctaggatt tactatagct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
      721 atctaaaaga ccgtggttta gaagaggttt tgttagtcgt aacagatggt ttaagtggta
      781 ttgacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
      841 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
      901 tcaaattagt tta
//
LOCUS       DANKAZ010000257          905 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000257 DANKAZ010000000
VERSION     DANKAZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.268"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            265..888
                     /locus_tag="PWY67_002956"
     CDS             265..888
                     /locus_tag="PWY67_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2059138.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          334 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaaa
//
LOCUS       DANKAZ010000258          883 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000258 DANKAZ010000000
VERSION     DANKAZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.269"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            85..354
                     /locus_tag="PWY67_002957"
     CDS             85..354
                     /locus_tag="PWY67_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2059139.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            347..604
                     /locus_tag="PWY67_002958"
     CDS             347..604
                     /locus_tag="PWY67_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2059140.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          336 a           97 c          168 g          282 t
ORIGIN      
        1 taagaagtta gaatattaat catttgctta attgtacaat ataatgtaca attgttttat
       61 agaaataaat aaggggtgaa aggaatggaa gcagtagctt attcaaattt ccgccaaaat
      121 ttacgtagtt atatgaaaca agttaatgag gatgctgaaa cacttattgt aacaagtaaa
      181 gatgtagaag atacagttgt tgtattatca aaaagagatt atgattctat gcaagaaacg
      241 ttgagaacac tttctaataa ttacgtcatg gaaaaaattc gtcgaggaga tgaacaattc
      301 tccaaaggtg catttaaaac acatgactta atcgaggttg aatctgatga ttaaggcttg
      361 gtctgatgat gcttgggatg attatcttta ttggcatgag caaggaaaca aaagcaatat
      421 aaaaaagatt aacaagttaa taaaagatat cgatcgttcc ccctttgctg gattaggaaa
      481 acctgagcca ttaaagcatg atttatctgg aaaatggtcc agaagaatta cagatgaaca
      541 tagactgata tatagagttg aaaatgaaac gatatttatt tattctgcaa aagatcacta
      601 ttaaccaatc ggaagtaagg aaagggtcag aaacttaaaa gtttttgatc cttattttat
      661 ttaccctagt catttaaaaa gctaatatag cttagtgttg attgttatta atgaatgtgt
      721 ttgttacgcg tattacggat ataaggttag taaaatcatt tctaaagttg aggaaaagta
      781 aatataaatg gcttaaattt caacaatttg aagttgaata gatatgttat aatactattg
      841 tagtgtggga tgttagttac taaaggatga cgcttatata tat
//
LOCUS       DANKAZ010000259          870 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000259 DANKAZ010000000
VERSION     DANKAZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.270"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..838
                     /locus_tag="PWY67_002959"
     CDS             <1..838
                     /locus_tag="PWY67_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL2059141.1"
                     /translation="SFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIV
                     MNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNK
                     RKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKA
                     KAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADT
                     ISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSR
                     DH"
BASE COUNT          333 a          102 c          167 g          268 t
ORIGIN      
        1 atctttttct atagttcaca atcaggttta ttttggcggg ggaattaatt tatggttagc
       61 caaaaagaat ggaatcagac agagaattgc tcatagccat gcaactgaag atggaaaatc
      121 tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc aaattgttgt tacaaaatgc
      181 aacagactac cttgccgtta gtcaagaagc tggtgaaagt ctgtttcaaa accatccctt
      241 tgagatagtt cataatggta ttgatttaga gctttatagt aaaaattcag aagctaaggt
      301 taataagaga aaagaattgg atatctcaat gagtactttt gttgtgggaa acattggacg
      361 attagagaaa caaaaaaatc aatcttattt gttagagatt tttttagaaa tactaaaaat
      421 gaattctgat tcttacttga ttttagtagg tgaagggtca cttagaaata aactgaaagc
      481 caaagcagta gaattggata tagagaaaag agtaaaattt ttaggagaaa gaaacgatat
      541 tcccgagtta ttatctgtga tggatgtttt tgtaatgcct tctctttatg aaggtctgcc
      601 aatggttgga ttagaagcac aagcagcaaa attaaaatta gtcttagcag atactatttc
      661 caaagataca aagttaacaa ataatgtgga ttttgtatca ttagatgaaa caccacaaga
      721 atgggcaaaa aaagtaataa aagagccgta tgctaataaa ataacacaag aactaaagag
      781 ttatgatgtt tcgtacacta ctgaacaaat ggagagtata tatagtagag atcattaaaa
      841 atgttcaaga tattttagga gtgattattt
//
LOCUS       DANKAZ010000260          868 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000260 DANKAZ010000000
VERSION     DANKAZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.271"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..>868)
                     /locus_tag="PWY67_002960"
     CDS             complement(<1..>868)
                     /locus_tag="PWY67_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL2059142.1"
                     /translation="LISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSIIL
                     SLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFR
                     KSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKT
                     PLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKV
                     DLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNY
                     AFLGIVLIFILSI"
BASE COUNT          334 a          147 c          108 g          279 t
ORIGIN      
        1 aatagatagt ataaaaatta aaacaatacc aagaaacgca taattcactt tacttctaaa
       61 aaatgttttg agtaaactaa agatatttaa cacatagaaa gcatagatac atataaaagc
      121 aattatacca cttgatacaa gtacttgaag aaatccatta tgcatgtttc catgtgctct
      181 ttttagttca ttcacatctg tagtatcaag atctactttt ttcaaaaatt ttgcatcgct
      241 attcccatag aaatcggtct ctccataacc aaataacaaa gttggtctta taactttaag
      301 cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg gagttatcac tagaatgctg
      361 taatattact tctttttctg gaagtggagt tttatctgat tcatttgact ctataacttc
      421 tacttttcta tcaatcgata caggaattgt tgctaaaaaa tccttagcat aattagtacc
      481 aaaagaaaaa tataccaata gaactaataa tatgcttacc atccatttga tttttacagg
      541 tagtacttct ctaatgctct ttctaaattt tgaattggca aacaacaaaa gaaataatac
      601 taaaaaagaa caaattacta cctgggcacc tctagacgaa gaaagtgtaa tataaaatat
      661 ttgtacgata aaattcatca tataaaaaac acgaatcgtt tttgttattt ttgaaattaa
      721 tacactaatt aaacttaaaa taatagaagt atatccaaac atagctccaa catttggact
      781 tgtatagaca ccaaataacc gatgttcaat aaaaccttgt cttgcaagta atcctggcgt
      841 tcctgttggg acatgataag aaatcaaa
//
LOCUS       DANKAZ010000261          868 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000261 DANKAZ010000000
VERSION     DANKAZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.272"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            373..642
                     /locus_tag="PWY67_002961"
     CDS             373..642
                     /locus_tag="PWY67_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059143.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
BASE COUNT          275 a          144 c          160 g          289 t
ORIGIN      
        1 tatttcatag gaacaatcgg ctggtttctt ctagcggtac ttttgattct gggcttagct
       61 tatctattat ttcctgattt gatacaaaat gtgtttgata ctgctttttg attgacctat
      121 taaaacgata taggggatgc tcctgcgatg caggggcttt ttttaatcaa tgttttgtaa
      181 taatttaggc cgtgccaatg agacttccta tagaattcga tgctcttttt tctttttggc
      241 tcttctgctc ttttttgtta tactagatta cagtggacaa aagaaagaat gatcgcatcc
      301 acttcaacga atatcaaaat ggaagaatta gtgcctgttt tcaggttact ttgaaaagaa
      361 aggaagacgc ttgtggaaac ttttgaaggt cttttaattg atacgtacgt tttacaagaa
      421 cagccaatga tgctgactgg gatcaacttg cagcttgagg accgtgtgat caatggcctg
      481 cttaaagatc gctatttggc aaaatatatc ttacgtttgc ccctaaataa atatgtgctg
      541 gaagtaagtg gcaaatggaa ccgtcggaac cagttggtca taaaaaaaat gttgataaaa
      601 aatatcgatg aatacattaa aataatcggt acaccagaat agcctttact gatcaaagga
      661 tactggttat aaaataactg cagtttctta atagaaaaac aaagaacaac agggaatcgt
      721 atcccttttc aaaaaaaact ttgcaaacgg ttctctttgt tatataataa cttagtacca
      781 aacattccca ttcaaacttt gtttaaatag ataaataaaa atctagtttg gtttgacgct
      841 attgcctccc acaatagcgt ctttttga
//
LOCUS       DANKAZ010000262          861 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000262 DANKAZ010000000
VERSION     DANKAZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.273"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..605)
                     /locus_tag="PWY67_002962"
     CDS             complement(<1..605)
                     /locus_tag="PWY67_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2059144.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKR"
     gene            complement(681..827)
                     /locus_tag="PWY67_002963"
     CDS             complement(681..827)
                     /locus_tag="PWY67_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDL2059145.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT          341 a          137 c           94 g          289 t
ORIGIN      
        1 tttcttttca attgctgttc atattgttct cgttgcctaa tagttattct aataaataaa
       61 aatagaacaa caaacatttg aatgattaaa aatattaaga caccaaacac aaaatgatca
      121 aatacttgat attgatgagc aacataagaa acaaataaac caattgtaag taaataaatc
      181 atgaatatag cagtaagttt agaactggac tgttcaacaa tcctatttat agctaatttt
      241 ctatataacc aaataaacat cagcaaaaac aaagaatcaa gcattatctg gaatactaca
      301 taaccatacc cttttatccc ttcaaatgaa aacatatacg tcataaccat ggaagtaata
      361 atagatctga aatcatcaat aagcatacat aatagtaatt tatttaaaag cactatatta
      421 gttttcttct tttcctttaa tataaaataa cttccgatta taggtatagc tgcggaaaca
      481 tcagtaaata attcaacaaa aaaagtgagt ggtatcaata aacatacaaa aagatatttt
      541 cttttgttaa gatcagtatt agtcatataa aataagagaa aaaagaaaaa gtttcccacg
      601 aacattctta atagataagc ttttgtatcg atagtaatca agagatcccc tcctgatttt
      661 aggatatcat aaaaaaaatt ttagttacac tttttaaata atgaaaaaac acattccact
      721 aaatttgatg ccggttttcc ttgaggtttt gttccagcac ctcctttaat ttttttaagc
      781 tcttgatctg aacattgttt agcatttagt ctattttttt cttccattac gattccccct
      841 acaaactata aatactttta t
//
LOCUS       DANKAZ010000263          856 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000263 DANKAZ010000000
VERSION     DANKAZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.274"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..596
                     /locus_tag="PWY67_002964"
     CDS             <1..596
                     /locus_tag="PWY67_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059146.1"
                     /translation="YNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPM
                     IDIYKEKKYIFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDN
                     GFIEDKNSSIPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEG
                     NPSCKKNAYDIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            593..>856
                     /locus_tag="PWY67_002965"
     CDS             593..>856
                     /locus_tag="PWY67_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2059147.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVI"
BASE COUNT          335 a          103 c          139 g          279 t
ORIGIN      
        1 tatataacaa aagctataat ttttattgtt tttttcctaa tatatataca ggaagagaga
       61 atacaataca aattccatta gttacagctg ggtcaagaac cgcattgcaa ctttcggata
      121 tttttaaccc tatgatagat atttataaag aaaaaaaata tatatttttt acgtctgttt
      181 atgattttga gggcggagaa cctgtcggag aatatgaatt agtatgcaaa atagcagact
      241 tagtaggaaa agataatcta attgttaaga cgcatcctag agatgtaaga acaatttata
      301 aagataatgg atttatcgag gataaaaatt catcaatacc ttgggaggcg attcaattgt
      361 caggcgattt taacgataaa atatttatga caattaattc aggaagtgtt atttcaggta
      421 gtacaatgtc tgcaaaacct gtacgcacgt attatatgta taaattgtgt aacatagaag
      481 gaaatccgtc ttgcaaaaag aatgcgtatg atattgagcg attgcttaag caaaaaacga
      541 tgaaaaacat tttaaagaat gtgaaaatag cagaaagctt agatgatata ctatgagcaa
      601 aactaaatat ttaattaaaa atatgggaat attaacaata agtaattttt cttcaaaaat
      661 tttagttttt ttgttagtcc ctttatatac aagtgtatta tccacaacag aatatggcac
      721 atatgatttg acaatttcaa cagccacatt attgtttcca attttaacct taaacattgt
      781 agatgctgtg atgcgctttt taatggataa agataaaaat aaaaaaaata tagcatctat
      841 agcgattaaa gttata
//
LOCUS       DANKAZ010000264          856 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000264 DANKAZ010000000
VERSION     DANKAZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.275"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            136..309
                     /locus_tag="PWY67_002966"
     CDS             136..309
                     /locus_tag="PWY67_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059148.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          286 a          143 c          104 g          323 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaac
//
LOCUS       DANKAZ010000265          805 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000265 DANKAZ010000000
VERSION     DANKAZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.276"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            186..>805
                     /locus_tag="PWY67_002967"
     CDS             186..>805
                     /locus_tag="PWY67_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2059149.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPVTYSITNPGVHDVKVLETLSEEANLPNILGDKGYI"
BASE COUNT          295 a          133 c          133 g          244 t
ORIGIN      
        1 tttcataggt gtttttaaaa gttacacaaa aaatagaata acaaaaaaat taacattgaa
       61 ttaataatag ttatacccgt ggtgctagtt aattttttcc tagaggaaaa tgtctaaaca
      121 tgttggtaaa tcaacaaaaa aacgcatata gtagtctcaa aactcccaag aaatgaggac
      181 tacatatgcg ttcatcaaaa tatactgaac attatacaga tacttttata acattcaagg
      241 aaattatgag aactgtttct aacatatacc acaattgtgt accagataag attaaaaatc
      301 gaagaaatac tgatcaactg aagcaacgcg atacagtgat tattgcttgt gttatatggg
      361 gcataattaa tggctatact agccaaagag ccacgtatag agcggtttgt tccgtcttat
      421 ttcctaatgg tgactttcct agtaggagtc gattcactcg cttaagttca aacttagctt
      481 acactatcaa gattattcga tactttttta tcaagaagct caccaaaggt gaactagtcg
      541 gaattataga tagttttcct agtccattat gtaaaccagt tagaaataga caagcaaaac
      601 tattgaatca aatagccaaa gttggctata actcaacaaa aaaatcttat ttttatggtc
      661 ttaaaattca tatgatcgtt actaagactg gctttccggt tacttactca atcacgaatc
      721 caggtgtcca tgacgtgaaa gtgttagaga ctttatcgga agaagcgaat cttcccaata
      781 tcctagggga caaaggatat attag
//
LOCUS       DANKAZ010000266          801 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000266 DANKAZ010000000
VERSION     DANKAZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.277"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..>801
                     /locus_tag="PWY67_002968"
     CDS             <1..>801
                     /locus_tag="PWY67_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HDL2059150.1"
                     /translation="TEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLM
                     VDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDM
                     NAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKN
                     WRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVE
                     AFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYT"
BASE COUNT          257 a          163 c          153 g          228 t
ORIGIN      
        1 tgactgaaaa agtatcttta tcttttattg cgaaaaattg tcaggtatct ttgaccaccg
       61 ttattcgtac gttgaaagag tttaaaagct acttaccaaa gcaatctaag aggattctcc
      121 ccagagtatt gatggttgat gaatttcgtt cgcacgcttc catagaagat aaaatgagct
      181 ttatttgcgc agatggcgaa acaggacaat taattgatgt cttgcctacg cgtaaattgc
      241 ctcgattaac aagctatttc ttaggctgcg ctaatcctga agaagtaaaa tttttagtca
      301 cagacatgaa cgccgcctac ttccagctta ccaaacgtat tctgccaaat gcgaaagtag
      361 tgattgatcg gtttcatatt atcaaacaca tgaatcaagc ctttaatgag ttgcgtatcc
      421 gtgaaatgaa tgaacttcgt aaagtaggac agaaaagtca ggcagaaaaa ctgaaaaaga
      481 actggcgctt tttgctaaaa aatcgtgcaa acatcaacca ttatgaatac aaaacatgga
      541 aaagttttcg agcaccaaaa tacccatttc ttacggaagc gatgatgatt gatcgattgc
      601 ttggcttttc tacgtccctg aaggaggcgt atccctattt tcatgaatta gttgaagcct
      661 ttcgagacaa agaccctgac ttatttttct ccttattggc agaacttccc gaaacgttag
      721 atgacggctt tcgggaaaag ctccaaaacc ttctgaccta tgaagaaggc atcaccaacg
      781 cgatgatcta tccttacacc a
//
LOCUS       DANKAZ010000267          749 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000267 DANKAZ010000000
VERSION     DANKAZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.278"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..352)
                     /locus_tag="PWY67_002969"
     CDS             complement(<1..352)
                     /locus_tag="PWY67_002969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059151.1"
                     /translation="MDKPFLDSVREYLSNPKSTLFPHEDYKIIMNYYLPIGAPQNSKI
                     KIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGL
                     DLKNLLCADDKIKKI"
BASE COUNT          233 a          131 c          109 g          276 t
ORIGIN      
        1 gtattttttt gattttatca tctgcacaaa ggagattttt taaatctaat ccttctttta
       61 tttcaaaagt tgaatttttt aaatctataa gagtaaaacc aattccttca tactgaattt
      121 ctgaaaatcg aatttttctc ccagttgata gatcaagggg cagaaaacct tttattacat
      181 tgtgagtgtc accatcattt acaatgtcat taggaatttt gattttagaa ttctgtggag
      241 cacctattgg aagataataa ttcattataa ttttgtagtc ttcatgagga aataaagtac
      301 ttttaggatt gctaagatat tctcgtaccg aatctaagaa tggcttatcc attgaatctt
      361 catccccatt taaactaaaa aacattccta caattgcttt tgcaactcta agtgaattca
      421 ttttctttat tgaatgtgtt aaatcagact taccattctt taaacttgta ttgttataaa
      481 ggaaattttt attgtcttct gcaaatctaa tatattcctc aacatactca ctaccaaaaa
      541 aactattaca tttactacat aacgtattgg aatggcaaca gtttttttga caaattttat
      601 aaggtgcaga acttctttcc gtatgctatt ccgattgtcc ttgacaatga gcctcctcgg
      661 atgtgacgat cttcggcagg agctaacagt taaagttaga ctgcctcgct agcgttagca
      721 catccgagct cattatcaag gattcgctg
//
LOCUS       DANKAZ010000268          733 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000268 DANKAZ010000000
VERSION     DANKAZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.279"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..>733
                     /locus_tag="PWY67_002970"
     rRNA            <1..>733
                     /locus_tag="PWY67_002970"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DANKAZ010000269          712 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000269 DANKAZ010000000
VERSION     DANKAZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.280"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..446)
                     /locus_tag="PWY67_002971"
     CDS             complement(<1..446)
                     /locus_tag="PWY67_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2059152.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DANKAZ010000270          703 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000270 DANKAZ010000000
VERSION     DANKAZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.282"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(158..637)
                     /gene="rlmH"
                     /locus_tag="PWY67_002972"
     CDS             complement(158..637)
                     /gene="rlmH"
                     /locus_tag="PWY67_002972"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL2059153.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT          171 a          138 c          111 g          283 t
ORIGIN      
        1 tttctatgtc tacattatct ttttaacagt atctattttt cttttgcaac ttacttggct
       61 accaaagcaa ataaaaaagt tatttataaa tttctcccta ccactaccta ttgctattat
      121 ttaaaatagg gataattttt tctcttaaaa aatatcatta tttgtggtat ggagcccccg
      181 cattgatccg gaaggcacgg tagatttgct cgacaaggat cagacgcatc aattgatgtg
      241 gatacgtcat ttttccaaaa gagatctgag cattgcttct tttcatgacg gcttcgctga
      301 gtcctaaaga gccgccgata acaaaaacaa ggtggctttt tccttggata cctaattggt
      361 cgatttgttt agcaaaagat tcacttgtag gatttttccc ttcaattgct aaggcataga
      421 cgtattcttg ttctttgatt ttcgctaaga tccgttcccc ttctttttct tttacttgtc
      481 tcatttgcgc ttcgcttaaa ttctccgggg ctttttcgtc aggaacttcg atcaattcga
      541 tttttgcata ggcattcagc cgtttgacat actcattgat accttggatc aggtattttt
      601 cttttaattt tcctactgag ataattttga tattcatgtt ttctctcctt tggtcttcat
      661 gctcactttg tttattataa tcaatttttt cgtctatatg ttt
//
LOCUS       DANKAZ010000271          702 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000271 DANKAZ010000000
VERSION     DANKAZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.283"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..>702
                     /locus_tag="PWY67_002973"
     CDS             <1..>702
                     /locus_tag="PWY67_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2059154.1"
                     /translation="HFKYLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFV
                     LIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPA
                     AGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITM
                     IVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGT
                     VASAIVNILLNMIFIP"
BASE COUNT          218 a           89 c          126 g          269 t
ORIGIN      
        1 ccattttaaa tatttgattt tatcttttat atctactttt ttgaatattg caatctctgt
       61 agctttgatg cagacattat attttgatga taaatatgtt ggcagagtag tcggcggaac
      121 actgtcattt gtgttgatag gtttagtaat ttatttaaga ataatgataa agggtgctca
      181 tttttttgat aaaaaacagt ggagatacgc tttaaaaatc tctttgccgc taatcccaca
      241 ttccttagca aatatagttt tagcacaatt cgatagaatt atgattaatt cttatttagg
      301 tcctgctgcg gggggaatat attcctacat tagtaacata ggtattattc taagcgtaat
      361 atggatgtct actaataacg cctgggttcc atggttctat ggtgagatga gtaaaaataa
      421 tgaaaaaaaa gtgaagaaag ttagtaacta ttatttagta gtctttactt tgattacgat
      481 gattgtaatg gtagtttcga ttgatttagc aaaatttatg gctccgaaag actattattc
      541 tgggttatct ttagtggttc cgataacctt aggctatttc tttcagtttt tatatagttt
      601 accagttaat gctgaatttt atgagaaaaa aacatcgttt attgcgattg gtacagtagc
      661 aagtgctata gttaatatac tattaaacat gattttcata cc
//
LOCUS       DANKAZ010000272          673 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000272 DANKAZ010000000
VERSION     DANKAZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.286"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..552)
                     /locus_tag="PWY67_002974"
     CDS             complement(<1..552)
                     /locus_tag="PWY67_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059155.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVL"
BASE COUNT          219 a          111 c           81 g          262 t
ORIGIN      
        1 tagcactgcc atataacatt caaaacattc ttctatagtt ggtttacttc cttgaggaga
       61 atgaaccaat ttattacgtt tttctatgaa atttttaatt tcctttctta tttctttcgg
      121 aaactcaata ttaaagtacc ctttaaacat gctccctaat aatttgtcta cagggggaga
      181 ttgtaaattc tcaaatatta gagttaaatc aggtttctct tgttgataaa attcctttac
      241 ccctatttca caggctgtaa ttatcgaaat aaacctatct ctagtatttg atatattttt
      301 acttctttta aggagtataa gagaaaaggg gatactttcg tgtccatgca tgtaattaac
      361 taattgttga gcttcagaag ttatatctct aattatatag ttaacggaat taggaagtgt
      421 ctttaactct aatgaaattt ctatccaatt actatcttct aatctgtata tgctcgaagt
      481 ataatttgat tcgtcagcaa gcatcgctac atcccaaaca ctgatcataa aatcttttaa
      541 acgttcaatc atttcagcaa tattgctaaa atcagctaat ttttctttag aataaaaaaa
      601 acatttatat cagaatcaat aaattctatt ttttttatta ctatatcgtc atcattatct
      661 tgaccattta tgt
//
LOCUS       DANKAZ010000273          659 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000273 DANKAZ010000000
VERSION     DANKAZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.287"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..324
                     /locus_tag="PWY67_002975"
     CDS             <1..324
                     /locus_tag="PWY67_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2059156.1"
                     /translation="SNLEINSNDLATYILRYDDKIIEIHLDYFGRVPTRKFQLFTLDE
                     CINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANEVLK
                     IAEEK"
     gene            325..>659
                     /locus_tag="PWY67_002976"
     CDS             325..>659
                     /locus_tag="PWY67_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2059157.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDL"
BASE COUNT          260 a           69 c          121 g          209 t
ORIGIN      
        1 tcgaatcttg aaattaacag taatgattta gcaacatata tattgagata cgatgataaa
       61 attatagaaa ttcatttaga ttattttgga agagttccta caagaaaatt tcaattgttt
      121 actttagatg aatgtatcaa tgttgactta ttaaataata aaataagttt tgaagaaaac
      181 attgttaaag aaatgcctga atctgctaat gatatgtatg agaaggagtt gaaatatttc
      241 tttgaaaacg tagtaacagg caaagaaaac tggaatgatt tatatcatgc aaacgaagta
      301 ttgaaaatag ctgaggagaa ataaatggaa attttattta ctatttgtgg tagagctggg
      361 tctaaggggt ttaaaaataa aaatttgaaa tatttggcag gaaagcccct agtccatttc
      421 gctttagcaa tgatagatat ctatcaacaa aaacatcctg aacataattg cacgacttgt
      481 ttgaatacgg acagtaatga gttagtagaa ttgacgacta agttaaatcc gtctgtgaaa
      541 attgtagaac gtaaacctga attagctaca gatactgtag ggaaaattga agtgatacgt
      601 gattcattct tacaactaga aagagaagat aatatatttg atttaattat agatttaga
//
LOCUS       DANKAZ010000274          656 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000274 DANKAZ010000000
VERSION     DANKAZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.288"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
BASE COUNT          220 a          126 c           87 g          223 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaataa gatacaacag tgatgattcc taataaagaa aagactacaa taatattttt
      601 ctaacttatc gccttcaaag aaatagtttc ttatttttca ctaaaataaa aaattt
//
LOCUS       DANKAZ010000275          649 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000275 DANKAZ010000000
VERSION     DANKAZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.289"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..>649
                     /locus_tag="PWY67_002977"
     CDS             <1..>649
                     /locus_tag="PWY67_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2059158.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DANKAZ010000276          635 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000276 DANKAZ010000000
VERSION     DANKAZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.290"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..270)
                     /locus_tag="PWY67_002978"
     CDS             complement(<1..270)
                     /locus_tag="PWY67_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059159.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="PWY67_002979"
     CDS             complement(276..>635)
                     /locus_tag="PWY67_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2059160.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DANKAZ010000277          624 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000277 DANKAZ010000000
VERSION     DANKAZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.291"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            13..>624
                     /locus_tag="PWY67_002980"
     CDS             13..>624
                     /locus_tag="PWY67_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059161.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSE"
BASE COUNT          215 a          100 c          133 g          176 t
ORIGIN      
        1 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
       61 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
      121 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
      181 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
      241 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
      301 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
      361 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
      421 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
      481 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
      541 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
      601 ggagtaactt cccaaaattc cgag
//
LOCUS       DANKAZ010000278          602 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000278 DANKAZ010000000
VERSION     DANKAZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.292"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            178..381
                     /locus_tag="PWY67_002981"
     CDS             178..381
                     /locus_tag="PWY67_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HDL2059162.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            381..>602
                     /locus_tag="PWY67_002982"
     CDS             381..>602
                     /locus_tag="PWY67_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2059163.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFID"
BASE COUNT          234 a           65 c          101 g          202 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc
       61 attgttatat aataaaatgt tatataataa aattactaat gatatttaat attacaagca
      121 atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg
      181 aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata
      241 gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg
      301 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
      361 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      421 atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta
      481 ctgatgtttt atttcaggta tataaaaaaa ttgatacagc aaaatatcct gaaattcttg
      541 taaataaatt agttaattat atttacatcg tgggttttga taataaaata cgctttattg
      601 at
//
LOCUS       DANKAZ010000279          573 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000279 DANKAZ010000000
VERSION     DANKAZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.293"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            79..456
                     /locus_tag="PWY67_002983"
                     /pseudo
     CDS             79..456
                     /locus_tag="PWY67_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
BASE COUNT          200 a           68 c           96 g          209 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcatcc aatagaattt gacaatgatg aaagattgct gctaatatat
      121 gcgtttatgg atgaaaattc tatcttttca actgataatt caacttatag aaaagtagtt
      181 caatgggaaa gtgataacaa tttaaggagt tacttgagtg atagagtatt tgatgtacta
      241 gaaaaattaa gtaaatcggg aattttaaag gaagaatacg attcttcttt tgatgagtat
      301 ccatcacgct atattttgat ttcagaggac tataagaaat ttaaaaggta ttttttaaaa
      361 aatgaaaaaa agatatctgt tattatggag aaatacaaag ctccaataca ggctgatcca
      421 ttttccaatt tggatgaatc agatttaccg ttttaaattt taaatagata atttcatttt
      481 ttagtagcag aggtataaac ttttttaatg tttgaattga aaagaaatgc taataggtcg
      541 actttatgtt tctcttgtgc aatcgtaaat gcg
//
LOCUS       DANKAZ010000280          569 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000280 DANKAZ010000000
VERSION     DANKAZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.294"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            145..543
                     /gene="spxA"
                     /locus_tag="PWY67_002984"
     CDS             145..543
                     /gene="spxA"
                     /locus_tag="PWY67_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2059164.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          201 a           88 c          111 g          169 t
ORIGIN      
        1 tagataactt tcattttttt aaaaaaatta tgaaaaaaag gtagctattt aaaagttgat
       61 tatgtataat agaaacagat gggagattat tatgatcttc tgttacaaaa aagtgattct
      121 tatcacgaaa aggagtggtg cacgttgtta acattatata cttccccaag ttgtacttcc
      181 tgcagaaagg ctcgtgcgtg gttgcaagaa catcagattc catttgttga acgtaatatt
      241 ttttcagaac cattgaacag ttctgaatta aaagcgattt tacaaatgac agaagacgga
      301 acagaagaaa tcatctcgac acgttccaaa gtattccaaa aactgaacat ggatttagat
      361 gatctgccgt tacaagaatt attggaactt gtgcaaaaca atcctggatt gcttcgccgt
      421 ccaatcatga ttgatgataa acgtctgcaa gttggtttta atgaagatga aattcgtcgg
      481 ttcctaccgc gcgatgtacg acaattagaa ttacgtcaag cacaattgat ggctggatta
      541 tagaaggata atacgaaact tggaaaaga
//
LOCUS       DANKAZ010000281          563 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000281 DANKAZ010000000
VERSION     DANKAZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.295"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(1..229)
                     /locus_tag="PWY67_002985"
                     /pseudo
     CDS             complement(1..229)
                     /locus_tag="PWY67_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          163 a          107 c          119 g          174 t
ORIGIN      
        1 aaactttccc gtgaaaaatc tcttgtttta ataagccaaa aaaattctcc ataagtgaat
       61 tatctaagca gttccctttc cgagacatgc tttgaaagat tttgtactcc ttcaatttgt
      121 ttctataagc tttcatttga tacgaccaac cttgatctga gtgaaacgta cgacgatagg
      181 gacaatcgtt agtggtctgg atagcttcct ccaaaccttc cattaccgca cgtgtttctg
      241 aatggtggta gcgaccgatt tgaacggcta gggcggaaat acggtgcaaa tgcacggtgg
      301 tttcatgcaa tgcttacagg gcgtattttg taagggtatg taacggagat aatcccgacg
      361 gtaatggatg tgagaggcca agccgacgct gacggtaggg caaacaaaca tagttgcact
      421 ctagcgatcg ttatttattt ttaacggtca aactagggta caactatgct taaaacactt
      481 cgcctcaact tctggtagca aaagtgattt tgaaagtcaa aatatttttt atgagttatt
      541 tataattcgc cgtttgtata att
//
LOCUS       DANKAZ010000282          538 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000282 DANKAZ010000000
VERSION     DANKAZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.296"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            262..516
                     /locus_tag="PWY67_002986"
     CDS             262..516
                     /locus_tag="PWY67_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059165.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
BASE COUNT          135 a           81 c           95 g          227 t
ORIGIN      
        1 atgggatcat cattctttcc tttttagtga atgcgatcgt cctcttttta tatgtattga
       61 attacttaga tatcaatcca ttgatttttt ctcgatagat aagacacatt cagcggaaca
      121 gttcctgctt atcagcagaa aactgttctg ctttttcatt tttcattgat ctattcttct
      181 tttattttcc tagcatttca tttgtgatgt atgcaataac atgttatctt ttattatatg
      241 aatcgttgag gagtccgtta catgattacg ttggtgagag ttttgttttg ggtgcctgcg
      301 gtcgcactgg tcgcaagtat tgtttatctt atgaattgga acaaagaacg gttttattta
      361 gctattttga ctcttccggc tatctacttt atgtggaaag tttttaatta caactacttt
      421 gaacccgata gtgtttttat agaagaatta tcaggactgg ttttgtcttt attgatcgtc
      481 attctttatt tgatacgttt aaataaaaaa cattgagtac tattgattgc atgtgtag
//
LOCUS       DANKAZ010000283          526 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000283 DANKAZ010000000
VERSION     DANKAZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.297"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="PWY67_002987"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="PWY67_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL2059166.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="PWY67_002988"
     CDS             complement(349..>526)
                     /locus_tag="PWY67_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059167.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DANKAZ010000284          514 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000284 DANKAZ010000000
VERSION     DANKAZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.298"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..132)
                     /locus_tag="PWY67_002989"
     CDS             complement(<1..132)
                     /locus_tag="PWY67_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2059168.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DANKAZ010000285          501 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000285 DANKAZ010000000
VERSION     DANKAZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.300"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
BASE COUNT          168 a           96 c           58 g          179 t
ORIGIN      
        1 tttttaaaaa taagaatgct aaatcactct acaataacgg cgaaagcaca aaaaataaaa
       61 aaattattac gttattaaaa aaacagatga aagatcaggt tttattagat ctcattcttt
      121 catcacttga agggatttaa tgaactactt tttcctcttt gtcgttttta tatattcgta
      181 ttccttcaaa cagtaggata gcctctttca atgcttgata tttgaactgt ccgttactta
      241 aaaatttaga tgtgccgccc tcttcctaga acatattctt ttttactttt tcattaattg
      301 acagaccaat cgaacgaaac tgtaaatttc gataggataa tgattggtca aactcattca
      361 ttttcaagac atctcgattc atattcaaga taggaatctt tttatcaaca tcactttatt
      421 cgtattttcc tctaccataa aagcttcacg ctccacaaaa ttgttttata cactactatt
      481 cagcccattc aacgaccaat g
//
LOCUS       DANKAZ010000286          485 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000286 DANKAZ010000000
VERSION     DANKAZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.301"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(62..259)
                     /locus_tag="PWY67_002990"
     CDS             complement(62..259)
                     /locus_tag="PWY67_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2059169.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DANKAZ010000287          452 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000287 DANKAZ010000000
VERSION     DANKAZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.302"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..>452
                     /locus_tag="PWY67_002991"
     CDS             <1..>452
                     /locus_tag="PWY67_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059170.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DANKAZ010000288          411 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000288 DANKAZ010000000
VERSION     DANKAZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.305"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..159)
                     /locus_tag="PWY67_002992"
     CDS             complement(<1..159)
                     /locus_tag="PWY67_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2059171.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNS"
     gene            complement(178..339)
                     /locus_tag="PWY67_002993"
     CDS             complement(178..339)
                     /locus_tag="PWY67_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDL2059172.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          139 a           76 c           44 g          152 t
ORIGIN      
        1 agaattagaa ctttcatata aagccttttc tatttcattg cttgatttca ttattgttaa
       61 acaagaatac aagcaagaaa gaactatagt taatatgata aaaattatta aagttctatt
      121 tttctttctt gtaatatatg ctatagcatt ttttatcact ttcctaacta ctcctctcta
      181 ttttcttact ccataactaa ttgcatccac tggacaagca tttttacatc gtccacatct
      241 gatacactcc agatgattgc aattctcaac tgaatcttta ttcatttggc aaactttttt
      301 acattttcca caattaatac acttatcctt atcctgaatc ggctacacta gactagacag
      361 aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat g
//
LOCUS       DANKAZ010000289          409 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000289 DANKAZ010000000
VERSION     DANKAZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.306"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..379
                     /locus_tag="PWY67_002994"
     CDS             <1..379
                     /locus_tag="PWY67_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDL2059173.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DANKAZ010000290          404 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000290 DANKAZ010000000
VERSION     DANKAZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.denovo.308"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..>404
                     /locus_tag="PWY67_002995"
     CDS             <1..>404
                     /locus_tag="PWY67_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2059174.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DANKAZ010000291        15582 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.guided.060, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000291 DANKAZ010000000
VERSION     DANKAZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.guided.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     misc_binding    101..317
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            365..3010
                     /locus_tag="PWY67_002996"
     CDS             365..3010
                     /locus_tag="PWY67_002996"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL2059175.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            3320..4633
                     /locus_tag="PWY67_002997"
     CDS             3320..4633
                     /locus_tag="PWY67_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL2059176.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            4623..5276
                     /locus_tag="PWY67_002998"
     CDS             4623..5276
                     /locus_tag="PWY67_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2059177.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            5331..5882
                     /gene="radC"
                     /locus_tag="PWY67_002999"
                     /pseudo
     CDS             5331..5882
                     /gene="radC"
                     /locus_tag="PWY67_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6013..7098
                     /locus_tag="PWY67_003000"
     CDS             6013..7098
                     /locus_tag="PWY67_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2059178.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7095..8987
                     /locus_tag="PWY67_003001"
     CDS             7095..8987
                     /locus_tag="PWY67_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2059179.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            8994..9371
                     /locus_tag="PWY67_003002"
     CDS             8994..9371
                     /locus_tag="PWY67_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2059180.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            9525..10307
                     /locus_tag="PWY67_003003"
     CDS             9525..10307
                     /locus_tag="PWY67_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDL2059181.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(10433..11164)
                     /gene="erm(A)"
                     /locus_tag="PWY67_003004"
     CDS             complement(10433..11164)
                     /gene="erm(A)"
                     /locus_tag="PWY67_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDL2059182.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11222..11281)
                     /locus_tag="PWY67_003005"
     CDS             complement(11222..11281)
                     /locus_tag="PWY67_003005"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HDL2059183.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            11673..12335
                     /locus_tag="PWY67_003006"
     CDS             11673..12335
                     /locus_tag="PWY67_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2059184.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            12570..12713
                     /locus_tag="PWY67_003007"
                     /pseudo
     CDS             12570..12713
                     /locus_tag="PWY67_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            12863..13063
                     /locus_tag="PWY67_003008"
     CDS             12863..13063
                     /locus_tag="PWY67_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2059185.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(13317..14996)
                     /locus_tag="PWY67_003009"
     CDS             complement(13317..14996)
                     /locus_tag="PWY67_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2059186.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(14998..15210)
                     /locus_tag="PWY67_003010"
     CDS             complement(14998..15210)
                     /locus_tag="PWY67_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL2059187.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
BASE COUNT         5232 a         2477 c         3185 g         4688 t
ORIGIN      
        1 cgagaactcg tcgtaattct ttcaaaaatt ttttagtact aaatgaagtg gttttttcac
       61 gaccacttgt tgactaaaaa aaaaaaccat tgtagaatga agcataacgc tgaacaagag
      121 gagtaactgg cacattcatt cttagagaga gaactatctg ctgaaaggtt ctcaatgaca
      181 accagtgaag gtagcttggg agtttctgtc ttgaatggag tagagataga cgagtcgtga
      241 tcgttaactc acttgagagg caataatgat tgattattgc aatttaggtg gtaccgcgta
      301 aatcatacgt cctagaatac gaaagtattt taggacgttt tttagtttaa aggaggaaaa
      361 cagcatgacg gaagaaaata acctatcaac aaaatataat ccgcaagaag ttgaagctgg
      421 acgttatcag cattggttag aacaagattt gtttaaaccg agcggagata aaaaagcaaa
      481 accttattca atcgtaatcc cgcccccaaa tgttactgga aaacttcatt tagggcatgc
      541 ttgggatacg accttgcaag atatgctgat tcgtcaaaag agaatgcaag gatatgatac
      601 tttatggctg cctggtatgg accatgcagg aatcgcgaca caagcaaaag tagaagcgaa
      661 gcttagagag caagggattt ctcgttatga tctaggtcgt gaaaagttca tcgatcaagt
      721 gtgggaatgg aaagaagagt atgcgagcca catccgcgaa cagtgggcta aaatgggctt
      781 atctttagat tacagtaggg aacgttttac attagatgat ggattatctg aagcagtacg
      841 taaagttttt gttaccttat acgaaaaagg tttgatttat cgtggtgaat atatcatcaa
      901 ctgggatcct caagcacgaa cagctctatc tgatatcgaa gttattcata aagatatcga
      961 aggcgccttt tatcatatga gctatgaact agcagatgga tcaggcgtag tagaaattgc
     1021 gacaacccgt ccagaaacga tgttgggaga tacggcgatt gctgttcatc cagaagacga
     1081 gcgttatcaa gcgctcatcg gcaaaaaagt gattctacct ttagtcaata aacaaatccc
     1141 aatcattgca gatgaatatg tagatatgga attcgggaca ggtgtagtaa aaatcactcc
     1201 agcacatgat ccaaacgact ttgaggtagg taatcgtcat gatcttccac gcgtaaatgt
     1261 tatggatgat gatggtacga tgaatgaact agcaggaaaa tatgctggaa tggaacgttt
     1321 tgctgctcgt aaagcaatcg tgcaagatct gaaagaaatc ggtcgattga ttaaaatcga
     1381 tacgatgatc cacagtgtgg gtcattccga gcggacaggt gtcgttgttg aacctcggct
     1441 gtctactcaa tggtttgtaa aaatggcgcc gttagcgaaa aaagccattg ataaccaaga
     1501 aacagatcaa gcagtggaat tcttccctcc aagattcaat caaacgtttt tacgttggat
     1561 ggaaaatgtc catgattggg taatctcacg acaattatgg tggggacatc aaattcctgc
     1621 atggtatcac aaagaaacag gtgaaatgta tgtagggatg gaagcaccag cggatagtga
     1681 aaactgggta caagatcctg atgttttaga cacttggttt agttcagctt tgtggccttt
     1741 ctcaacaatg ggctggccag atacggaaag cgaagattat aagcgatact tccctactag
     1801 cactttagta accggttatg acatcatttt cttctgggta agcagaatga ttttccaaag
     1861 cttggaattt actggcgaac gtccatttga gaatgtcttg attcatggat tgattcgtga
     1921 cgaacaaggg cggaagatga gtaaatcatt aggtaatgga atcgatccga tggaagtaat
     1981 cgaaaagtat ggagcagatg ctctccgctg gttcttatcg aatggttctg caccaggaca
     2041 agatgtccgc ttcagctatg aaaaaatgga tgcttcttgg aactttatca acaaaatctg
     2101 gaatgcttca cgtttcgtta tcatgaatat cgaaggaatg accgtagaag agatcgattt
     2161 atctggagaa aaatcagtgg ctgaccggtg gatccttact cgtttgaatg aaacgatcga
     2221 acgagtaact gagctatttg atcgttttga atttggtgaa gcaggtcgac aactgtataa
     2281 ctttatctgg gatgattttt gtgactggta catcgaaatg agtaaagaag tactttatgg
     2341 agatgatgct gtttcaaaac aaaccacccg tagcatctta gtccatacac ttgatcagat
     2401 cctacgttta ttgcatccaa tcatgccatt cgttacagaa gaaatatggg aacatattcc
     2461 tcatcaggga aattctctag tagtggcaga atatcctgtt gtacgtccag agtttgatga
     2521 tgaaacagct tctaaaggga tggaagtatt gaaagaattg attcgagccg ttcgaaatat
     2581 tcgttcagaa gtgaatacac cattgtctaa accaattacg ttgctaatta aaacaaacga
     2641 tgctaaaatc gatcaatttt tagttgaaaa tacaagctat atcgaacgct tctgtaatcc
     2701 cgaagagttg acgatatcaa gtgagatcac tgcgccagat ctagcgatgt cagctgttct
     2761 gacaggtgca gaaatcttct tgccgttagc aggattgatc aatattgaag aagaaatcaa
     2821 acgattagaa aaagaactgg aaaagtggac aagcgaagta aaacgtgtcc aaggaaaact
     2881 ttctaatgaa cgttttgttt ctaacgcgcc ggaagaagta gtagaggcag agcgtgcaaa
     2941 agaaaaagat tatttagaga agcaaacggc tgtcaaagaa cgaatcgaaa gtttgcgtac
     3001 tattcaataa gaatagaaaa agagagtgtg aaatcagcta gctattacta aaagctagct
     3061 gattgaacat cgttacagga aagagtttga gcctcactct tagcttcgat tcataaggag
     3121 atgtgacata aattttgtcg catctctttt ttagtctact cttattatgt aggagaagga
     3181 gggaggtgga tcatgtatca agagtcaata aagtagttga taaaatcgtt cctctctctg
     3241 tttcttttct ctttgtcata cttttatttt tcttatgtgt tctatttgtt ataataaaag
     3301 agaaatgggg gaattgttga tgacgattga agaagcaata gaatggatcc atagtcgatt
     3361 accatttggt tcacgtcctg gccttgaccg agtcgaagct ttattagaag tggtagggca
     3421 tccagagaga aaagtaaaaa cgatccatat cgctggaaca aacggaaaag gttctactgt
     3481 agcttatttg agaagcctat tagaagaggc tgggctgaca gtaggtacat ttacttcacc
     3541 ttatatcgaa acgttcaatg aacggatcgc aatcaatggc aaaagcattc cagacaatga
     3601 tttgatccag cttgtaaaaa aatatcagcc actgatcaaa caacttgatc aatttgaagc
     3661 agtagctggt attactgaat tcgagacatt aacagcaatg gcatttgatt attttttgat
     3721 gaagaaagtg gacgtagcga ttatcgaagt tggcttaggc ggcttattgg attctaccaa
     3781 tgtagctgta ccaatgctga cgggaataac aacgattgga cttgatcata ccgaaatact
     3841 tggagataca attgaagaaa tcgctgcaca aaaagctgga atcatcaaac aaggcgttcc
     3901 agtagtaaca ggaaatattg ccccagaagc ttttactgtc atccaacaaa cagctaaagc
     3961 aaaaaatgca ccgctttatg tatacgggga aaattataaa ataaactatc tgcatcctga
     4021 tgagatgtgg ggagaagtat ttgaattttt taatgaaata ggaaagatcc ctcaattaac
     4081 cactccgctt ttagggcaac atcaagctga aaatgcagga gtagccatcg aattgtattt
     4141 tgtttactgt caattggaaa agctgccttt ccgagaaaaa gatgttttga agggattgaa
     4201 gaaatctcga tggccaggaa gaatggagcg tttgagtaac gagccattag ttgttttaga
     4261 tggtgcccat aatacgcatg caatgaaccg gcttgttgag aatttgaaaa aagaattcaa
     4321 aggatatcaa atcaacattt tgttttccgc cttacgaact aaaaatgtta gagaaatgct
     4381 agaacgattg cagcaagtac caaatgtcca tatttatttg acgacctttg aacatccaaa
     4441 agccatcgaa ttaagtgaag tatcagattt agcaagtgag agagtatcaa tcgtttcatt
     4501 gtggcaattc ggattagcag atatattgga aaagatgaca aatgaagacg tattagttgt
     4561 gaccggttca ctttatttca tttcagaagt tcgccattta ttaatacaat taggaggaga
     4621 tcatgaagta taatgcagtg atttttgaca tggatggttt gctgttcgac acagagttag
     4681 tgtattacga agccacacaa gtagtagcag acaagatggg ttttccttat gataaagacc
     4741 tgtatttaaa attcttaggt gtttcagatg aagaagtatg ggcgaattat catatgattt
     4801 tctcggaatt tggcaaaaca agtgtacaaa gattcataga tgattcatac caggaaacgc
     4861 ttgaacgttt ttcgcaagga gcggtacaaa tgaagcccgg tgtgttggaa ctcctctctt
     4921 ttttagaaga acatcagatt cccaaagttg tcgcttccag taaccaacga gcagtcattg
     4981 aacttttatt gaagaagaac aatctgtttg aacgatttga gacgattgtt tctgcagaaa
     5041 atgttcaacg agcaaaacca gacccagaaa ttttcttgtt agctaatcag aagctaggta
     5101 cagcgaagaa agaaacattg attttagaag attcacaaaa tggtatttta gcagcttctg
     5161 cggctgagat cccggtagtc atggtaccag atctgcttcc accatcccaa gaattgatca
     5221 aaaaaacagc tggggttgtt tcttctttac atgaaatccc tgcattgtta aaataaaaat
     5281 ccatctcttt tcgtatctta ttttagataa gatagaaagg agatgttttt ttgggaaaac
     5341 gattgatcaa agaagtacct actagctctt taccaaggga acgtatggaa atttatggcg
     5401 cggaagcttt atcggatcag gagcttttgg caatcttact gcgtaccggt caacaccctt
     5461 atagtgtgat gtcgattgct ggaaatctgt taaaaacgtt tggaggactg gcctctttac
     5521 gacaggctac tttgcatgag ttagaagaga ttcgagggat aggacgcgtg aaagcaatcg
     5581 aaatcaaagc cttgatcgaa ttaggtcgac gaattcagac ggatcatgaa agagcggctc
     5641 caccggttcg taccagctac gaattggccc agcaattgat tctggaaatg aaagatcaca
     5701 aacaagaaca tttggtttgt atttatttgg acacgaagaa tcaagtgatc ttaaaaaaaa
     5761 cagtgttcat cggttcgctg aatcagtcag tcgctcatcc tagagaaatt ttccattatg
     5821 ctgtacgtta ttgtgcagca aggatcgttt tagcgcataa tcatccttct ggtaatgttt
     5881 taaccatagt ataataaata atagtagtta aagtagttta agaggagata ttaaagggat
     5941 attttacttt aaataaaaat taacaaggtt tataatttgt agtagttaaa gtatttaaag
     6001 aggtgggaac atgtgaaggt tcaaaggata gaagtggaga ataagccgta tccattgtat
     6061 ttattactag ataaagaata ccagctaata gaaccagtaa tgaaatttat taaatactta
     6121 gataatactg gtaagtctcc taataccatt aaagcatact gctatcattt aaagttgctg
     6181 tacgagttca tggaacagag aggtgttatt cttaatgata ttaactttga gttgttagca
     6241 gacttcgtag gttggttgag atatccttca gcatcaaatg taattgatct tcagtcaaaa
     6301 aaagccataa gagaagaaac gacagtgaat acaattttaa atgtagttat gagttttctt
     6361 gattatttaa gtagattagg agaatttaaa tcaattgatg tatttaaaca agcaaaggga
     6421 agaaatttca aaggattttt acatcatgtt aataagggta gataccaaaa gaatgtctta
     6481 aagttaaggg ttaaaaagaa acagataaga acattgagat cagaggaagt taagcaaatt
     6541 attgatgctt gtcatacgaa aagagataaa ttaattttaa tgcttatgta tgagggtggt
     6601 ttaagaatcg gtgaagtgtt atcgcttagg cttgaagata ttgtcacttg ggacaatcaa
     6661 atccatttaa cacctagaga tgttaatgtt aatgaagctt atattaaatt aaggaaggaa
     6721 agaacaatac atgtgagtaa agaacttatg tcactttata cagattactt gatatatgag
     6781 tatagtgagg aattggagca tgattatgtt tttatttcct taaaagaagg ctattttggg
     6841 aaaccactaa agtaccaaag tgttcttgat ctagttagaa gaatagttaa aaggactgga
     6901 atagaattta catcacatat gcttcgccac actcacgcaa cgcagctaat tagggaagga
     6961 tgggatgttg cgttcgttca aaagagatta ggtcacgcac atgttcagac aacgttaaat
     7021 acctatgttc atctttcaga tcaggatatg aaaaatgagt ttaataaata cctcgagaga
     7081 aaggagcata agaaatgaat gcttctagta aaaggaaaat tattagtcag agtgagatta
     7141 gcaaaaaaat agctgtaatg aatgaagaaa tgcaggggtt ttgggctaat aatagttggg
     7201 atataagaaa atgtccacat ccttctgcca tagaattaag taagaatcct gctttaagga
     7261 atcgttgggt tcgttttgaa cgtgttaaaa atctgtggtt aagaacagaa ttgaaatatt
     7321 tttattttta ccatttaaac aatggaatat ggaatgcaaa aactgtctgg attagaaaag
     7381 gaacagtaat taataaaatg ttagatttct tagatttaaa gtatcctagc attacttcaa
     7441 ttactgaagt tcctattgaa aaagcaatga cggagtatag aacttatttg acaaaacggg
     7501 gtgttagaat taccactact aattataaga ttactgctaa tcaagaaaaa acacctgtaa
     7561 aagctaattc ctactatgtt actaatctaa aacaatttat ggagttttat gagaactttt
     7621 attttgatgg agaggagtgg gataaagacg tttgggatag acgtaactta cctttgccag
     7681 atgataaggt taacccaaca caatatgaat atacaattaa ctttaaaggg tttcggaata
     7741 catattttaa acaacttgta aaaagatatt gtaagttgag attgaacatg gatagctttt
     7801 cctatgtaag tgatattgcc caaagactta aagagttctt taattttctg gacatgaaat
     7861 ttaaacaagt tcagagagta caccaattaa cgagagtgga aattgaagca tatttaagtg
     7921 aactaaacat gatgggaata aaacctagta caataactgg gaggatctct atattggaag
     7981 gactatttag tacccttctt aggctagaat gggatgatgt tccttccaaa atattaattt
     8041 attctgagga ctatccgaaa ataccaagag caaaaccacg ctttatagat gaattcgtcc
     8101 tagagcaatt gaacagtcat cttgataaat tacccgaata tatagctacg atgactatga
     8161 ttgttcaaga atgtggaatg aggataagtg aattgtgcac cttgaaaaaa ggctgtctat
     8221 tagaggacaa agatggagat ttctttttaa agtattatca atggaaaatg aaaaaggagc
     8281 atatagttcc aatatctaaa gaggtagctt tacttattaa agttcgggaa gataaagttt
     8341 cagaggaatt tccagatagt gaatacctct ttccaagaaa agatggatcg ccattaaaac
     8401 aagaaacatt tagaggtgag ttaaataaat tagcttatga gcaaaatata gtggataaat
     8461 caggtgagat ttatagattc catgcccatg cctttcgcca tacagtagga acaagaatga
     8521 ttaacaacgg gatgccccag catattgtgc agaaattttt ggggcatgaa agcccagaaa
     8581 tgacaagcag atacgctcat atctttgatg aaactctaaa aaatgaattt actaaatttc
     8641 aggaaaaact ggttaccaat aatggagatg tgcttgatct agatgaagat aatgaagtcg
     8701 atgatgtaga gcttcaatgg ttcaagaaaa atataaatgc acaagtgctt ccaaatggtt
     8761 attgtagatt gccagtagta gcaggtggtt gtccacatgc gaatgcatgc ttagattgca
     8821 ctcacttctg taccagtaag caattcttac cacagcacga agaacagtta gagcgtacag
     8881 aagagttatt agccatagct aaggataaac aatggcaaag acaagtagag actaatagcc
     8941 gtgttaaaga gcgtttagaa caaatcattg gaagtttgac ggggtaatta tcaatggata
     9001 aacaagttag aaatacaaca gaaattgtac gtttggcgaa gcagaaatca aaaaagacaa
     9061 gggaaaaagt agacaaagcg atttctaaat tttcgattga aggtaaagtt attaatttta
     9121 attcaatagc aaaggaagct aatgtttcta aatcatggct ttataaggaa cacgatatta
     9181 ggcaaagaat cgaatccctt cgtgagcgtc aaataacagc aaatgtagtc tcaaaaccca
     9241 agaaaagttc tcgttcggag gaaatcctta ttaaaacctt aaaaagaaga gtaatggaat
     9301 tagaaaaaga aaataaaaaa ttacagaacc aaattcaaaa attatatgga gatctgtata
     9361 ataaagaata attattaatc tgtagacaaa ttgtgaaagg atgtacttaa acgctaacgg
     9421 tcagctttat tgaacagtca tttaggtata tgtccaatct agggtaagta aattgagtat
     9481 caatataaac tttatatgaa cataatcagt tacgaggtga aatcatgagc aatttgatta
     9541 acggaaaaat accaaatcaa gcgattcaaa cattaaaaat cgtaaaagat ttatttggaa
     9601 gttcaatagt tggagtatat ctatttggtt cagcagtaaa tggtggttta cgcattaaca
     9661 gcgatgtaga tgttctagtc gtcgtgaatc atagtttacc tcaattaact cgaaaaaaac
     9721 taacagaaag actaatgact atatcaggaa agattggaaa tacggattct gttagaccac
     9781 ttgaagttac ggttataaat aggagtgaag ttgtcccttg gcaatatcct ccaaaaagag
     9841 aatttatata cggtgagtgg ctcaggggtg aatttgagaa tggacaaatt caggaaccaa
     9901 gctatgatcc tgatttggct attgttttag cacaagcaag aaagaatagt atttctctat
     9961 ttggtcctga ttcttcaagt atacttgtct ccgtaccttt gacagatatt cgaagagcaa
    10021 ttaaggattc tttgccagaa ctaattgagg ggataaaagg tgatgagcgt aatgtaattt
    10081 taaccctagc tcgaatgtgg caaacagtga ctactggtga aattacctcg aaagatgtcg
    10141 ctgcagaatg ggctatacct cttttaccta aagagcatgt aactttactg gatatagcta
    10201 gaaaaggcta tcggggagag tgtgatgata agtgggaagg actatattca aaggtgaaag
    10261 cactcgttaa gtatatgaaa aattctatag aaacttctct caattaggct aattttattg
    10321 caataacagg tgcttacttt taaaactact gatttattga taaatattga acaatttttg
    10381 ggaagaataa agcgtcctct tgtgaaatta gagaacgctt tattacttta atttagtgaa
    10441 acaatttgta actattgaaa atagaaagaa attgttcctt cgatagttta ttaatattag
    10501 tgacatttgc atgcttcaaa gcctgtcgga attggttttt agtgaaaaga acacgatatt
    10561 cacggtttac ccacttataa acaaaagatc gatacttttt gtagtccttc tttgaaatca
    10621 atggttgatg tcgttcaaga acaatcaata cagagtctac acttggctta ggatgaaaat
    10681 atagtggtgg tacttttttg agcattttta tatccatctc caccattaat agtaaaccca
    10741 aagctcgttg cagattttgc aatcttttcg caaatccctt ctcaacgata agatagctat
    10801 atttagcctg actttcaaag gtaattcttt tgacaatatc cgtactgatg ttataaggaa
    10861 tattaccata tatcttatag tttatatgtt ttgggaagga aaattttaga atatccgttt
    10921 gaatcacttt tatattctca gaggggttta ccgcttcttt agtcacttga cataagcctc
    10981 catcaatttc tatagcagta actgatcgac tcattttgac tagctctttg gtaaaatgtc
    11041 cttttcctga tccgatttct attacgttgt cttgtttact gatattcgtg tgattcaata
    11101 tttcttttac atgcttttta gaagtaataa aattttgcgt gtctttaggg tttttctggt
    11161 tcattataac cttctcctta ctggttataa tgaactgaca ttaagcgttc attataacca
    11221 attaattttg atttggttga taacgaactt tattaataac aaatatagaa aaagtaccca
    11281 tacttttatc tcctttccat ttttttcatt atgaatgaga taaagtaatt tctactactg
    11341 cgatactggt gcacataatt gaaacctcct tatgtaactg aatatgattg tatcatcttt
    11401 tcattttaga ataaaccttt atatgtaatt ttgcacttga cacttcagat ggacgttttc
    11461 ttcaaaataa tatgttttga aacttgataa gagaagccac tgtctttact aaaaagtaaa
    11521 ggctgcgata aaggacagtg actttaataa agttgcactg ttgtattgtc tttggataaa
    11581 attgtctaaa gggaagtttt atccccaacg aaaaaacatc taaagtggta agtaatgaaa
    11641 gaatgtcgat aaagtaggaa tgggggatct ttttgaatac aatagaatca cagtttgata
    11701 aggttgcaga agattacgat ttcgtgaatg agcttttgaa tgattattca ttctttgtgt
    11761 ctaatatgtc tccaaagaaa ggcagagcat tagatatcgg atgtggctcg ggtttgttag
    11821 tggagaaatt agcaagttat tatgatgaag tggtagggat tgatatttct aatcaaatgc
    11881 tcgatcttgc caaatctaaa cgtcaactaa caaatacggt ctatctgaat atgaatgcag
    11941 aacaacttaa ttttaatgag aagtttgatt ttattgtaag ccgaacaacc tttcatcatt
    12001 tggatgatat agccagcgta atacagcaaa tgaaggaact gttgaatgag gaaggaagaa
    12061 tagttattct tgataatgta tctgaagtcg agacaccgcc tacctatgtt tataaattag
    12121 gggcaatcca agagttttta ccgcattgtt tcaaatttgg aataaaaaat gcgattagaa
    12181 tttataatca caacacatct aaatcatggc ttgagcattt agcttcagat aagtacctat
    12241 ctgaacaaaa ttattacgat ttatatgaaa agttgttgcc tggatgccaa ttccataaaa
    12301 tgggttgggc aatgggggtt gtctggacaa aataacttaa agctgtggct ttgaaaagtt
    12361 gaatttgtga ttgttttcag agctatcggg tgctttagta taaataacaa tgaataaaaa
    12421 atcagcctgt atcttatgtt actattaagt aatttaagat acaggtttta ctatttttga
    12481 aataagtacc ttccacttca aatacatgta ttcaaagtga aagtattgat ttaaatgatt
    12541 cttttgtaat ttactcttga taatatgaaa ttccatcgca gcaggatatg aattttacca
    12601 aaaggataca aaagtgtgga gaaatgatgg ggatcactgt actggaccac ttgatcatcg
    12661 gacgcaaacg atattttagt ttgagagaag aaggaatgat ggaagagaag taagttgctt
    12721 tcttgcaaaa aaaatgaagc aagtgtacaa ttatattgta attttgtttg ggaatagaag
    12781 ttgaaacatc tgttgcacct ctatcgtaca cgagcaatgt tacaatttaa attagtgctt
    12841 ttggcacgag gaggatatta ttatggaaaa cggaacagta aaatggttca acgctgaaaa
    12901 aggatttgga tttatctcac gtgaagatgg aagcgacgta ttcgttcact tctcagctat
    12961 ccaaggtgac ggattcaaaa cattagaaga aggtcaagca gtgactttcg acgttgaaga
    13021 atcagaccgc ggcccacaag ctgcaaacgt tgtaaaagct taattttatc tgtaaatcaa
    13081 attgatggta gacatcaatt tttacgagag aacaaagcga gtaacaaaaa tacctgccac
    13141 aagagacaat ttctcttgtg gcaggtattt ttttgtatga aaaagtaaat aaatttaaga
    13201 ggctatgaaa gagccacctt gtatcaagga ataagaacaa acaaaaatgg cttctcatgt
    13261 ttttcatttg ttttctctta tattcaggac aaggtgctga tttcataacc tcttatttat
    13321 tcttcatctg gtgtcattac cattggcaaa atcattggat gtctttctgt acgttcgaat
    13381 aagaaaggct gaagcgcgcc agtcatcgct tctttcaatt tatattctga acagtcaggt
    13441 ccattcaaac attctcttaa agcgtggaat aagattcgct gagcttcatt gatcatttct
    13501 cctgattctc gcatgtagac aaatccacga gagagaatat ctggaccagc caagatttct
    13561 tttttcttga tatccactgt cgcgacagct aatacaagac cttcttctga aagcaggtga
    13621 cggtcacgta aaacgacatt accaatatca ccgatcccgc taccatcgat atacacatct
    13681 ccagccacaa aatgcccagc aggacgcgca gaatcgctag ttaacgctaa aacatcgcca
    13741 ttttccatga tgaagcagtt ttctgcagga acacctacat cttgtgctag agaagcatgt
    13801 actttcaaca tgcggaattc tccgtggaca ggaataaaat attttggctt catcaaacga
    13861 agcattaatt tttgttcttc ttgtccaccg tgaccggaag tatggatgtt attgattttt
    13921 ccgtgaatca cttctgcacc agcttcagac agcaaattga tcaaatgatt gacacttgtt
    13981 gtattaccag gaataggtga acttgaaaag atcactgtat cgtctggctg gatttggatt
    14041 tgacggtgag taccattcgc gatgcggctc aacgcggcca tcgcttctcc ttgagaacct
    14101 gtacatagga tcatcgtttc attggcaggc aagcgattga tttcgtgtgc atctacaaat
    14161 gtgccgtctg gaactttgat atatcctaaa cgttgaccgt tgacgatggc attttccatg
    14221 cttcgtccga aaacagcgat tttacgacca gttgccacag cggcatctgc tgcttgctgc
    14281 aaacggaaga tgttagaagc aaagctggca aaaatgatac gtccttcgat ttttgaaaag
    14341 atcttcaaaa tagaatgccc aatcgttttt tctgatttag taaatgttgg aatctcagca
    14401 tttgtactat cagataaaag gcacaagact ccttcttccc cgatacgagc cattttatgc
    14461 aagtttgctg gttcgccaac aggagtgaaa tcgaatttga agtctccagt cgctacgata
    14521 tttccagaag gtgttttcac cacgatccct aatgcatctg ggatactatg ggtcgttctg
    14581 aaaaagctga cagaagtctt tctaaaacgg atgatcgtat cttcattgat ctcatggagc
    14641 tctgcatctc gaagcaagcc atgctcatct aatttgtttg tgatcaacgc taatgcgaga
    14701 ggaccagcat agataggaat atttgcttgt ttcagcaagt agggtattcc gccgatgtgg
    14761 tcttcgtgcc cgtgagtaat caccaaggct tttactttgt gtaagttttg tatgatgtag
    14821 ctgtagtcgg gaatgacata gtcaattcca agtaaatcgt cttccgggaa tttgatcccg
    14881 gcatcaatca aaatgatttc atcttggaat tgtaccccat acgtattttt cccgatttca
    14941 cctaatccac caatagcaaa gacgcctgtc tcgttatttt tgatggaaag tttcatatta
    15001 aaactccact aatgagaaat cagcattttc tttttcgtac tctagatgac ttccttctag
    15061 aagttgaacg aattcaatat tgtatggggt attttcttcc acacgttggc gagcgtctac
    15121 atcgcttttt gcctcaatgt aaagagattt tgtttcttcc cgtttagggt tgcgtacttt
    15181 tgtttcttga taataaactt tatagatcat tgttttgtct cctttatctg cgtaaagcag
    15241 tgatttagct agttgatttt tttagacaac acaaaacgag gggacaagcc acctggtttt
    15301 tcatatgctt tgactagaat actggattcg agtcaaagag acaagcctcg tttttgctta
    15361 tctgttcgga tattttttca accgtacctt ctgtataaaa aatatacaac aattaattgt
    15421 tattctagca taaaacagca aataagtata gaaatcaact agattgaaag aaaaaatcaa
    15481 tgtttatcat cccgctataa tcataatgac ttttttatga gagaagtgtt ctattaatag
    15541 gctcgttgtg ctatcatttt tagtaatgaa aatgtaacaa aa
//
LOCUS       DANKAZ010000292         9529 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.guided.094, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000292 DANKAZ010000000
VERSION     DANKAZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.guided.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(<1..561)
                     /locus_tag="PWY67_003011"
     CDS             complement(<1..561)
                     /locus_tag="PWY67_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2059188.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELK"
     gene            689..1258
                     /locus_tag="PWY67_003012"
     CDS             689..1258
                     /locus_tag="PWY67_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL2059189.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1325..2062
                     /locus_tag="PWY67_003013"
     CDS             1325..2062
                     /locus_tag="PWY67_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2059190.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2293..3561
                     /locus_tag="PWY67_003014"
     CDS             2293..3561
                     /locus_tag="PWY67_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2059191.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4048..4512
                     /locus_tag="PWY67_003015"
     CDS             4048..4512
                     /locus_tag="PWY67_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2059192.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4496..5125
                     /locus_tag="PWY67_003016"
     CDS             4496..5125
                     /locus_tag="PWY67_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2059193.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5479..6957
                     /gene="msr(C)"
                     /locus_tag="PWY67_003017"
     CDS             5479..6957
                     /gene="msr(C)"
                     /locus_tag="PWY67_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2059194.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7004..7450
                     /locus_tag="PWY67_003018"
     CDS             7004..7450
                     /locus_tag="PWY67_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2059195.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(7649..8164)
                     /locus_tag="PWY67_003019"
     CDS             complement(7649..8164)
                     /locus_tag="PWY67_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2059196.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8281..9483
                     /locus_tag="PWY67_003020"
     CDS             8281..9483
                     /locus_tag="PWY67_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2059197.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3034 a         1672 c         1985 g         2838 t
ORIGIN      
        1 tttcagttcc tgcggatgga tgactgtttg ctttgctaaa ggatgattcc gatgtatcaa
       61 aagtccccaa cgatcttggg ctggcagtcg aaatgactgg tatttttgtt tgtttgtcgg
      121 ttcgattacg agtccaaaat caagtaatcc gctatccagt ttgtctatga tatcatctgc
      181 attcccgcta tataaatgaa actggatatc aggatgcgcc ttgatcaacg aatgaaggct
      241 atcagaaatg aaagagattg ctttagtttc gcctccgcca atgtagattt ctccagagat
      301 gattgcgtca tttgttaaat tgttcgccgt tttttctacc aacgacaaga tttcttttcc
      361 ttttgaataa agatatcgac cagaatccgt caactgtatc tctcgggagc ctcgagtgaa
      421 aagtgtcaca ccaagttctt cttctaaatc tttcagttgt tttgagagag taggttgcgt
      481 gatatgcaga gattttgctg ctcggctgat atttttttct ctagctactg ctaaaaaata
      541 cgtcagtata cgaatatcca ttttttccct cctgttttta aaaaagcata ccactaagta
      601 tagttaaaag gaatagttaa gtatgtttta taagtattag acatcataag ccgtccgtta
      661 taaagtaagg ataaaaggag ggagcaaaat gacaagcctc atcgaaaaac aaatcgtcaa
      721 taaagagatt cgagtaacgg atccgctctt tgaagtgatc catcagatcc aagcggaaaa
      781 cgaagagcct ttagcagcgc tgaatactgg gtaccatgaa ccagcagata tccgcaaacg
      841 gctagaaaat atcattagtg acaaagtaga tgatacggtg actgttctgc tgccttttta
      901 tacagatttc ggcaaacata ttagtatagg gaaaaatgtc tttatcaatc gtcaagtgat
      961 gttcgttgac ttaggtggca tctgtttaga agattcagta ttgattggtc ctcgtgtcaa
     1021 cctcatcacg gtgaaccatc taaccgatcc aaaagaacga agaggcttgt cagtaaagcc
     1081 aatccacatc aagaaaaatg cttggatcgg agcaggagca actattctgc caggcgtaac
     1141 gattggcgag aatgccattg ttgcagcgaa tgcaactgtt acgaaagatg tcccagacaa
     1201 taccatcgtt gcaggaatcc ctgccaaaat cgtcaaacct gtcgaacgga ttatttgaac
     1261 agtaagatga aataagagat agtgagtata agcaagagat ttttcaaaac aggaggagaa
     1321 tagaatgaat gatttatcag aaaaagtgat tgtcatcatg ggcgcttcaa gcggaatcgg
     1381 tgaagcaaca acgaagctat tagcagaaaa aggggctaaa ctagttatag ctgcacgccg
     1441 agaagatcgc ctaaaagcaa taaaagaatc tttaccagaa gctgagctat atattcaaac
     1501 agcagatgta agagattttg cacaagtcca agcagttatc gatttagcga tggaaaagtt
     1561 cggtcgaatc gatgtattgt acaataatgc aggaatcatg ccaaccgctc cattagttga
     1621 aggtcaccgt gatgaatggc aaaatatgct tgatatcaac atcatgggtg tgttaaatgg
     1681 aatttccgcc gtacttccaa tcatggaaaa acaaaaatct gggcatatca tttcaactga
     1741 ttctgtggcg ggacatgttg tttatcctga ttccgccgtt tattgcggaa caaaatttgc
     1801 tgtccgtgca atcatggaag gattacgtca agagcaacgg caaaataaca tcaaatctac
     1861 gatcatctct ccaggagctg ttcaaactga attatatcaa accatcagcg acaaaaaggc
     1921 tgctcttgaa cttcatgaag cgcaaaaaga atggggatta acatctgaag atatagcttc
     1981 tgctgtagca tttgcgatag aaacgccaga ccggatgtct gtcagcaaca tgattatccg
     2041 tccgacgaca caggaagtat aatccataac ggataatatc cagagagaat tacttgttaa
     2101 atagttattc aaacgaccat caatttaagg cgtgaatgga gaagaatatt cttcatttac
     2161 gtctttttat gcagaaaaag caaatttatt aaaaagaaac aaatgttatt taaatatgga
     2221 cattgacttg cttttacttt tttagttaca ctgttattta ggaaaagttt cataattcaa
     2281 aggagaaaaa agatgaaagc aaagaattta ttatttagcg ttattctttt aggaaatcta
     2341 gcagttgcca acacaacagt gctagctgaa gaatcgacag aaactgtcgc aactcagaca
     2401 tcgagttctt cagaaacagg aattgaaaac gatacagaaa gtactttgca gtcagctact
     2461 gtctcagaaa atacaatttc aaccgaagaa ccaacaactg aaacaacaga acaaacacaa
     2521 accgaagaca gtacatcagc agaagctcag aacaatccag agaatgcaga agaaatcacg
     2581 aaccaattga cagatgggca aacattgaca catacccatc aagcagattt gtctgaattc
     2641 gaaaactata ctggctatgc tttttatgct cgctcatctg ctaattctca acaagcgttc
     2701 attgatagta ttgcctcaac tgcacaaagt ttggcaagtg ccaatgactt atatgcatca
     2761 gtcatgattg ctcaagccat cgttgaaagt ggctggggga acagtacttt ggcttctgcc
     2821 cctaattaca atctatttgg gattaaagga agctataatg gccaatctgt tacgatgcct
     2881 acttcggaat atgtgaatgg gcaatggatt acggtgaatg ccgcttttag aaaatatcct
     2941 tcgtacaaag aatcattgca agataacgta acggtattga agacgacttc ttttcagcca
     3001 ggcgtttact actattccgg tgcatggaaa agcaatacga actcttacaa agatgctacg
     3061 gcttggctaa caggtcgtta tgccactgcg cctaattatg gttctacatt gaacaatgta
     3121 atcgaaacct ataatctgac acagtatgat acagcaccaa cttcaacttc tccgatgtat
     3181 cgtctgtata accgtcatac aggagaacat ttatatacat tgaatgccgg agaaaaaaat
     3241 tatctgccaa ctgtaggctg ggaatatgaa gggattgctt ggcaagcacc aaacagcgga
     3301 caacctgtct accgtttata caatccttac agtggagacc atcattacac aatggcacaa
     3361 tctgagatca actctttaac aaaaatcggt tggcgttatg aaggtctaag tttttattct
     3421 ggtggttcca agccaattta tcgactattt aatccaaatg aaaaaactgg tacccatcac
     3481 tataccttga gtgctaagga aagagattgg ctaacaccga tgggctggag atatgaagga
     3541 ataggatttt atggttatta aaaagaggta gtgaaaaagc tgccctgcat caagtagtaa
     3601 gaacagccaa acaaaaacag attttcatgt tttttgtttg gttgaactta ttaccgcaga
     3661 tgcaagtttt tgaacaccgt ttactcaaaa aaggttgtga caacatcttg tcataacttt
     3721 tttattactg tttaagcata ctgatccaat ttctaatatt cgacgggatt cttgcaaaag
     3781 gcgatttcgg aaatttttgg cggtgtaaaa ttatctactg cagaagagct atcttctata
     3841 gtttgataag aatcaataat cttggtttta gcgttgtgct ttctttatca ttttttccgt
     3901 tcattctaca aaaaatttct tttctctata cttttctatt gagagcgttt tttttattac
     3961 acagctgatt ctttgacttt tttctttttt tattctatta ttgacaagtc aatcgttgac
     4021 gagtcaatta ttagaaagga ggaaattatg tctttagaag agattagtca ccttttatat
     4081 caaatcaaat tagccgatca aaaaatcgtc cagatctttg aagaaaatat cggtataagt
     4141 cttactagat acgagatcat gatgagactg aaagaagaaa atccattgct acaaagcagt
     4201 ttgcaagaaa agttgcagat tgatcaagga gcagttactc gtcatctgaa ggtattggaa
     4261 gcaaatggct atgtttcgcg tcagagaaat cctcagaaca accgggaagt gtatgtagcg
     4321 ctaacagaaa aagcccagaa gaaaatcaag cattgtgaaa tggaccataa agatacaggt
     4381 tccgtgctat ccccggcttt cagcgatgta gaaatgaaac aatttctaac acttttagat
     4441 aaattgaata cccaactttg caatataaca acagaaacga aagaggttaa taataatgga
     4501 acaaaaaaat gatttttcaa caattatgac tggtagaaga tccgttcgcg tatatgatga
     4561 aacatacaaa atcccacacg aagaaatgct ggaaatgatc aaagaagcga cgactgcacc
     4621 ttcttctgtc aatatgaaac catggcgctt tgtggtcgct gaaagcgagg aagcaaaaga
     4681 aatcttgcgt cctttgatcc gtttcaatac gagacaaaat gatacttctt ctgcaatggt
     4741 catgattttt ggcgatatgc aatgttacga atatggcgaa gaaatctaca accaagctta
     4801 cgaatcaggg aagatgccaa aagaagtcag agatcaacaa ttagctgcga tcattcctta
     4861 ttacaagagt ttttcaagag aagaaatgaa cgatgtagtc aaagtcgatt ctagcttagc
     4921 ggctatgcaa ttcatgttag ttgcgcgtga tcatggctat gaaacaaatc caattggtgg
     4981 attcgaagca gatcagttgg cagaggcatt tggtttggat aaagaccgtt atgttccagt
     5041 tattatttta tcaataggaa aagcggtaga agaaggttac gagtctgttc gtttagcacc
     5101 agaaaagatc actatgttcg aataaaaggt tttcagttac tattcagtag ctattgcttt
     5161 ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg gaaaaatgac tgcatcgatg
     5221 aaattacgtt tcgaactttt gaataacaac taaataaatt caaaagacat gtataaacaa
     5281 caatttcatg gatggagtat gtccattttt tcgaacgaca gtagaatagg tgaaagctat
     5341 gacagatttg gtgatttagt catagttatt tcgttcagag taggaaaaga tgtgatgcag
     5401 tttttttgca aacagatttt tctgctcttt tttagtatcg ataattgttg ttttacatga
     5461 aataggagtg taaaacatat ggaaaattta gcagtaaata taacaaatct acaagtcagc
     5521 tttggcaacc agctagaact atctattgat tctcttcgtg tctatcagca agatcgtata
     5581 gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat agccggtgaa
     5641 ctttttcccg atcatgggaa aatccaaaca gagatcacct tcaactacct gcctcaatta
     5701 acctatctcg ctgaagcaaa ggacctaaat ttggaattag ccagtcattt ccagttaaga
     5761 ctggaagaaa cttcagagcg gaaatggagc ggaggggaag aacgaaagat cgagttgata
     5821 cgtcttcttt cttcttatga acaagggatg cttctagacg agccaacaac ccatctagat
     5881 agaaaaagta ttgatcgact gattgaagag cttcgttatt attatggtac gctggttttt
     5941 gttagtcatg atcgctattt tctagatgag ctagcatcga aaatctggga agtaaaagac
     6001 ggagaaatcc gagagttttc ggggaattat agtgcctatc tcactcaaaa ggaattggag
     6061 aaaaagactc agctacgaga agcagagtcg atcatgaaag agaaaaaacg attggaaaaa
     6121 tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag taaaaagaaa
     6181 aagcaacaaa tcagactgga tcggttgtct tcctctaaac aaaaagacag tgtacaaaaa
     6241 gccatccaaa agaatgcgaa aacattagag agaagactcc aaaaaatagg agaaacaacc
     6301 aagccgcagc agatgaaaca aatccgtttt ccagtaccaa aatctcttga actccacagc
     6361 cgctatccca tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac attactggta
     6421 aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga aaatggttca
     6481 ggtaagacaa ccttattaga acatatccgc aaacaaggag aaggaatcct tctctctccg
     6541 aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa tgacatctga agaatccatc
     6601 attcgttttg tcatgagaca aacagagttt tcagaatcgc ttgtccgtag tttgctgaat
     6661 cacttagggt ttgctcagga aactttgacg aaaccgttat gtacattaag tgggggagaa
     6721 gcgacccgtc tgacgattgc tttgcttttt actaagccaa gtaatgtgtt gctgttagat
     6781 gaaccgacta attttattga tatggcaacg atcgaagctt tagagaagct aatgcaaata
     6841 tatccgggaa cgattttgtt tacttctcat gattcttact ttgtcgagcg tacggctgat
     6901 gaagtttatg aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa tttttaaatg
     6961 tgctacgatg attttcgtga caaatttaaa ggagtggtac agattgccta caaataaaaa
     7021 agaaggaata atttttacaa caatgatgtg ttttttgatg gtcttgggga tgagcgcgta
     7081 taatttatgg ctgcatcaag atcttcattt atcaagtttg ttgattggtc tagtgcctgg
     7141 attcgtggtg gctttcattt tggatgtctt catcatcggt gtgctggcga aaaaaattgc
     7201 ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta tttcatgctt
     7261 gatggtttta ggaatggtga catgtatgtc tttattcggt gtgatcatgg aacaaggaat
     7321 acctgatcaa ttattctctg cttatatgaa tgcttggaaa atgaatgtga ttgccgcttt
     7381 accgttgcag ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag caattcaaag
     7441 taatgaatga tggaacactg ttatttgaaa aaaggccttg gcatgctttt tgtcatagtc
     7501 tcttgaatgc agttctagaa aaatagtgat ccgaacgata ggttttggcc tgtcgttcgg
     7561 atcgcttttt cttttccaac tgtattgctc ttttattgaa gataatttgc tcttcaatag
     7621 atatttctag aaaggttctg tttatccctt actcaaaaaa ctgctgcatt tgttctctta
     7681 cttgctgaca ccattggagt tcctgctgac aaagcataat tctgcgctgg atcacaacat
     7741 aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt caattttttt tgattttttt
     7801 gcatgatttt gttcagctgt tgttctctag tgaatagcag tccagtagct gttgttttat
     7861 cgagcgtcga aaaagcatag atttttgcta gaaattcatc tttttgcgta ggagcgggag
     7921 tttcctcttt gatccattcc tctacaagag cacgtccttc atcagtcaag tggtagatct
     7981 ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata accctgctgg tctaacttgg
     8041 caagcgttgt atagatctga ctgtgatggg cttcccaaaa aattgtgatc agctgcttca
     8101 attcataccc agaacgtggt ttggataaaa gcacgcataa taatatatag gctaaagtat
     8161 tcataaaaat cctacctctc gtcttatttt ctttattata tcacagtaaa gtttccacgt
     8221 tttacatgtg catattgaca tgtaaaataa atcatgtatg atgctaatag agagaaaggg
     8281 atggaaatat tggagaatca aatagatata caagaacaaa caaatgaaga aacaaagggg
     8341 aaaagttcag cgctagttgt agcagctacc tgtatgatcg cttttatggg agtgggtcta
     8401 gtagacccaa tcttaaaaac gattgctgca cagttaaacg ctacgccagc tgaaacgacc
     8461 ctgttattta ctagttacat gttagtcact ggtgtggtga tgctatttac aggtttctta
     8521 tctagtcgga tcggtttgaa aaaaacattg atgacaggat tgtttatcat cgttgtattc
     8581 gctggtcttg gcggattatc tgggaatatc ggaatgctga ttgggttacg tgccggctgg
     8641 ggtgtcggga atgctttatt tgtctctact gctttagcaa cgatcgtcag tattttagtt
     8701 ggtaacacag aaaaagcaat catgatgtat gaagcagcac ttggctgcgg aatggctgtt
     8761 ggccctctag tcggtggggt cttgggcagt ggttcatggc gttatccatt ttttggggta
     8821 gcatttttga tgttttgcgg ttttatagcc ctagcggtac ttttacctga aactgagaaa
     8881 ccaaagagaa aagtaggcct ttgggaaggt gtcaaagcgt taagcaatca taaacttcgt
     8941 tcagttggac taatcgcatt gttatataat ttcggattct ttactttact tgcgtatgcg
     9001 ccatttttat tgattggcta ttctgaatta gaagtcggtt ttgtattttt tggctggggt
     9061 gtgcttctgg ctattgcttc gattttcgtt gcaccggctc ttgagcggaa gtttacgact
     9121 tatcggacaa ttttagcagc atttgtctta ttcattattt gtttgctgtt attaggagct
     9181 ggaacttcta tgccagtcct tgtaccgatc agcattgtgt tagcaggatt tttccaagga
     9241 attatcaata cactgttgac gacgattgct atggaaatac caggtctgca acgaaacgtt
     9301 gcttcgtcca gctatagttt tgtccgcttt ttcggtggag ctttagcacc atttattgca
     9361 ggtgtgattg gtgaacatat tggtgaaagc tatgcgtttt attttgcagg tgccattgtt
     9421 cttatcagtg tatgttgtat catttatcac cgtaattatt ttgctacaaa tgaagaagaa
     9481 taagaaatca gaggttgtga aaaagctgtc ctgcatcaag tcataagaa
//
LOCUS       DANKAZ010000293         8061 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.guided.109, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000293 DANKAZ010000000
VERSION     DANKAZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.guided.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            128..1564
                     /locus_tag="PWY67_003021"
     CDS             128..1564
                     /locus_tag="PWY67_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2059198.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="PWY67_003022"
     CDS             1753..4200
                     /locus_tag="PWY67_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2059199.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="PWY67_003023"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="PWY67_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2059200.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="PWY67_003024"
     CDS             complement(5690..6793)
                     /locus_tag="PWY67_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2059201.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="PWY67_003025"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="PWY67_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2059202.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2696 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DANKAZ010000294         7882 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.guided.110, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000294 DANKAZ010000000
VERSION     DANKAZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.guided.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..1043
                     /locus_tag="PWY67_003026"
     CDS             <1..1043
                     /locus_tag="PWY67_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2059203.1"
                     /translation="EDRKGRKINVPIADGGENTAEAIYYALRGTWRTIDGMDLLFRQR
                     KISYLLTTWQGKNTAVIESAQALGLDLLDKPTDQTIRLASSYALGELIKDALLQEVEQ
                     IIVTLGGSGCSDGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRRNVLVSKVKLIIAA
                     DVTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWEVDLDDIPGSGAA
                     GGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQQTIHGKVPYGVA
                     VLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKILG
                     QTLSRFFTILPK"
     gene            1301..2803
                     /gene="eat(A)"
                     /locus_tag="PWY67_003027"
     CDS             1301..2803
                     /gene="eat(A)"
                     /locus_tag="PWY67_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2059204.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2831..3397
                     /locus_tag="PWY67_003028"
     CDS             2831..3397
                     /locus_tag="PWY67_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2059205.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            3608..4669
                     /locus_tag="PWY67_003029"
     CDS             3608..4669
                     /locus_tag="PWY67_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2059206.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4672..5568
                     /gene="murQ"
                     /locus_tag="PWY67_003030"
     CDS             4672..5568
                     /gene="murQ"
                     /locus_tag="PWY67_003030"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2059207.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5582..7564
                     /locus_tag="PWY67_003031"
     CDS             5582..7564
                     /locus_tag="PWY67_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2059208.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7629..>7882)
                     /locus_tag="PWY67_003032"
     CDS             complement(7629..>7882)
                     /locus_tag="PWY67_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL2059209.1"
                     /translation="NGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNKNFNNQYC
                     LTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         2624 a         1389 c         1776 g         2093 t
ORIGIN      
        1 gggaagatcg taaaggaaga aaaatcaatg tgccgattgc agatggcgga gaaaatactg
       61 cagaagcaat ctattacgcg ctcaggggaa catggcgtac cattgatggg atggatctct
      121 tattccgtca gcgaaaaatc agctatttat tgacaacttg gcaaggaaaa aacaccgcag
      181 tcatcgaatc tgcacaagca ttggggttag atctacttga taaaccaacg gatcaaacta
      241 tccgacttgc ttccagttat gccttaggag aacttattaa agatgcactg cttcaagaag
      301 tagaacaaat tattgtcact ttaggaggaa gcgggtgtag cgatggtggt ctaggattac
      361 tccaatcctt gggtgcttct ttagaagggt acacagaagg aaatccttta ttatctacta
      421 aaaaacttca ttttgatcga agaaacgttc ttgtttctaa agtaaaattg attatcgctg
      481 cagatgttac caatccatat acaggtcaac aaggagctgc ttggcaattt ggaagacaaa
      541 aaggaggaat agatacgacg ctagctttct tagataaaca agcaggaaag attgctcagc
      601 aaataaaaga tttctgggag gtggatttag atgatattcc aggaagtgga gcagcaggcg
      661 gtattggtgg agcccttttt ttgttgggag cgaagatggt ttctggtttc gatttagtca
      721 gccaaatcac tggattggaa aaaaagatcg aacaagcaga tgtcatcatt acaggagaag
      781 ggcggatcga tcagcaaacg atccacggaa aagttccata cggtgtagca gtgcttgcaa
      841 aaaaatatcg aaaaccaata atcgctattt gcggaagtag agaccaagag ctagaaggac
      901 tttctgattt tctcccaatt gtcttaagta ttcatttggg tccaataggt ttagaagaag
      961 caatggaaca caggcaaaca ttagaaaaaa caaaaatctt aggacagaca cttagcagat
     1021 ttttcaccat cctcccgaag tagttgacaa aatcattttc tttatgctat cgtaatttca
     1081 aatataggac aaagtgaggg atccgtgcat tgaaaaatta agtcgattga tgaaagctgc
     1141 attcagactg taccttgtgg agacaaggaa gcagtgtgca gattttcggg attggcttgg
     1201 ttttttcgat gtatgggtct ttttgtgtgt atccaaccct tgcatcctct ttagccagtt
     1261 ctgaaaatag gactggtctt tttttacgag gtgatggaaa atgtctaaaa tcgaaataaa
     1321 aaatctgaca ttcggctacg acagccaagg cacattatta tttgaacaag caaatctaaa
     1381 ttttgacaca caatggaaac taggacttat cggacgaaac ggtcgaggaa agacaacttt
     1441 actgaatatt ctacaaaaca aactacctta tcaagggcaa gtaatccatc agcaagaatt
     1501 tgcctatttc ccgcaacaga caaaagataa agaacgttta acctattacg tgttaaatga
     1561 tattacggat tttgagatat gggaaatcga aagagagctc caattgatgc aaacagatcc
     1621 tgaaatctta tggagagaat tcagcacact atcgggggga gagaagacaa aagtcctact
     1681 ggcactttta tttgtggatg acactcattt cccgttaatc gatgaaccaa cgaatcattt
     1741 ggatatctct ggtagaaaac aagtagcggc ttatttgaaa aagaaaaaac aaggcttcat
     1801 cgtggtcagc catgaccggg gatttatcga tgaagtagtg gaccatgttt tagcaatcga
     1861 aaaaagtcaa ctggaacttt atcaagggaa tttctctatc tatgaagaac agaaaaaact
     1921 tcgtgatgaa tttgaaatgg ctcaaaatga aaaattgaaa aaagaagtca gtaggctaaa
     1981 gaaaacagca gctgaaaaag ccgaatggtc tcgttcccga gaaggagata aaacaaagaa
     2041 acaagtcgga ttcatcgata ctgaatctag acgagtgaat aaaggagcag tgggtgctga
     2101 tgctgcacgg acgatgaaac gatccaaagc aatcgtgaat cggatggaga cccagatcag
     2161 cgagaaagaa aaactattaa aagatatcga atatatcgat tctttgacga tgaatagcca
     2221 agcgtctcac cataagcgac ttttaagcgt agaagatctt caattagggt atgaaaatct
     2281 gttattcgag ccaattcatt ttacaatcga gcctcatcag cgggtggcga tttcaggtcc
     2341 taacggtgca ggaaagtcat ccattatcca ttatcttctg ggggcattca acggcaaggt
     2401 tataggagaa aaataccagc caaaacatct gagcattagt tatgcaagcc aaaattatga
     2461 agacaatcga ggaacgttgg cggaatttgc agagaaaaac caagtagact accaagcatt
     2521 tttgaacaac ctccgaaagc ttgggatgga aagagatgtt tttcataaca agatcgagca
     2581 gatgagtatg ggccaacgga aaaaagtgga attggctaaa tctttatcac agccagctga
     2641 actatataca tgggatgaac cattgaatta tttggatgtc ttcaatcaag aacaattaga
     2701 acaactgatc ttgaacgtga aacctgccat gttactagtg gaacatgatc aaaccttcct
     2761 ggataaagta tctactgaga ttatttctct tgagagaatc taacccttgt aatctcaaga
     2821 acaacaaggt atggttaagg ggaactttgc caacgaagga gagaatcaca tgctttatgt
     2881 aacgagacac ggagaaacaa cttggaacgc acaaggatta gtttgcggac gggcagatgt
     2941 gccgctgact gaaaaaggac agatgcaggc acagaaacta gcagaaaagg tagtagattt
     3001 gcctgttcca atcacgaaaa tcatccattc ccctcttcaa cgagcaagag atacagcaca
     3061 agcagttgct gatcgtctca gcttgccgct gaccgttgat gaacgattgg tagaaatgga
     3121 ttttggggat tatgatggac tgccaagcaa agatgagaac tttcaaaaag cacgtttagc
     3181 gttcgctgta cgattcccaa atggggagtc agtcttagat gtgtatgcac ggatcgttcc
     3241 tttattgaaa gaatgcatcg aagacgaaga aaatgtctat ctgttagttt gccataatgc
     3301 cttgataaga gtgatcaatg cttattttca tccgatgcct aatgaggggt tctttacatt
     3361 catggtagat aatacagaat tggtttctta tgagtaatta tgtttttgtg ttatttaaat
     3421 aaaatatttc aaggcactgc ttttgcttag cagtgccttt tttaggttat gacaattctt
     3481 ttgataatat aaactcaacc tttagaaatg aaatttaatt attttaataa aagtattgaa
     3541 attttgtttc tttagatata tactctagtc atagaaatga agacggatac agaaaaagag
     3601 ggataatatg tttggctttt caatatttat gaacgattct ttgtcagaag agaagaaaca
     3661 gtatatccgc caaatggcat ccaatggctt tgtcggtatt ttcacttcca tgcatatacc
     3721 agaagatgat gtttcggcat atcaaaagcg attgatcgaa ctaggaagcg aagcgaaaaa
     3781 gaataaacta gaattgatgg tcgatatttc aggagaggca ttggaacgag cgggtttttc
     3841 attagaagat ctaacgcctt tgaaagcaat tggtgtaaca ggtttaagaa tggattatca
     3901 tatttccaat cagcagattg ccgactggtc acatcaattg aaaatcagtt tgaatgctag
     3961 cacgatcacg cctaaagata tcgatgaatt gaaagaagca gaagctgatt tctcacagat
     4021 tgaagcttgg cataattatt atccacgtcc cgaaacaggg ctggacaaag aatggtatca
     4081 gaaaaaaaat caatggctca aaattcaagg tctccttgtc caaggttttg taccaggaga
     4141 tacagagttg agaggcccac tatatcaagg attgccaact ttggaagaac atcgagggat
     4201 ccatcctctt gcaggcgctc tagatctatt agcaagtaac acggatattg tttacatcgg
     4261 agatgcaggt ctgtcagaaa acgttcagga gcaatttgct tctttccaaa aagaacagac
     4321 agtcttgctc catacggaac ctgtagatga agaattttac gaatatatat tagggaagca
     4381 tacgaatcgg caagacgatg caagagatgt catacgaagc gtagatgcac gttttcgaga
     4441 gattccgcct atcccagcaa gaaatacagc tacgcgtatg aaagggtcta ttactttaga
     4501 taacgaaaag tatttgcgct atatgggtga gatccaatta accaaatatg atttgccagc
     4561 ggatgaaaaa gtgaatgtag tagctaaagt tatcaaagaa gagctaccat tgatcaacca
     4621 aatcaaagct ggtatgaact atcaatttat aagaaaagaa ggaagataaa aatgagcaaa
     4681 atcaatttag agaacctaac aacggaacgc agaaaccaag aaacttttgg attagatgag
     4741 atgagtattt ctcaagccgt tcaactaatg aacaaagaag atcagaaagt ggccgctgcg
     4801 gttgaaaaag aactgacaag tatcgaacca gtgattgcta acacgattga gtcctttaaa
     4861 aaaggtggac gtctgattta tatgggagcc ggaacaagtg gaagactagg tgtgttagat
     4921 gctgctgaat gcgtaccgac attcggtgta gaaccggaaa tggtcatagg tctaattgct
     4981 ggtggagaag aagcaatgac tgtagctgtc gaaggggccg aagattctta tgagctaggg
     5041 caagaagatc taatcaagct gaatttgaca aaaaatgata tagttattgg tatcgcagct
     5101 agcggtcgta caccatatgt gatcggtgga ttagattacg caaaatcgat cggtgctgtc
     5161 acaggaacga tttcttgcaa taaacaagcc gaaatcagta aacacgcgga tttcccaatc
     5221 gaagtcgatg caggtccaga atttttgaca ggctctactc gattgaagtc aggaacagca
     5281 caaaaactta tcttgaatat gatttctact atcagtatga ttggtatagg taaagtattc
     5341 aataacttga tggtcgatgt caaaccaaca aatgaaaaac taatcgaacg ctcaaaacga
     5401 atcatcatgc aagcgacaga agctgattac gaaacagctg aaaagtactt tgaagaagca
     5461 gatcaaaatg ttaagctggc tatcgtcatg attttgacaa atagtgataa agaggaagct
     5521 caagcaaaat tagctgaagc aaacggattt gtaaaaggga ccttatgagg gggaaaagac
     5581 catggcaaaa aacgaactga ctttggaaga actggcaaaa caaatttata aaggggcagg
     5641 cggcatgggc aatgtagaaa gcattgtcca ctgcatgact cgtgtgcgta tgaagatcat
     5701 caatgatgat cttgtaaatg tctctgaatt aaaagcgatt ccaggagtgc ttggtgtagt
     5761 agaagatgaa cagcttcaag tcatcatcgg acctgggaaa gtgaacaaag tagcacagca
     5821 aatggttgat ttggcaggcg tgaaattagg agaaaaactt tcttctgtaa gtacagctac
     5881 agcaaattca tcagcatctg gaaaagaccg tgtaaacgaa cgggcacaag aaatgaaagc
     5941 ggcacaaaaa tcgaaacaaa aaccatctaa aatcaaatcg gtattaaaag acatttctaa
     6001 tatctttgtg ccaatgattc ctgcatttgt cggtactggg atcgtggcag ggattgccgc
     6061 agttttagca aacttagtca cagccggaac acttgatgca gcaacatggc aacaatatat
     6121 cgatgtgatg aacatcttga aaaacgggat gtttagctac ctagttatct atacaggtat
     6181 caatgctgca caagtttttg gtgcaacacc aactcttggt ggagttatcg gggccgttgt
     6241 gatgctgaca gggatgaatc cggaagcacc actgaaaaac ttatttactg gtgaagctct
     6301 tgcagctgga caaggtggga tcctaggtgt catcttcgct gtttggctat tgtcaatcgt
     6361 tgagaaaaaa ctgcataaag tagtacctga tgcggtagat atcatcgtta caccaactct
     6421 ttcattgatt gccatcggtc tgattgaaat tttcttcatc atgcctttag caggtttcat
     6481 ttctgatggc atggtcggtg ggatcaactg ggtacttggc gttggtggag catttgccgg
     6541 cttcgttcta ggcacattat tcttgccgat ggttatgttc ggactgcatc aaattttgac
     6601 tccaatccat gtgcaaatga ttgatgaaac tggtcgtacg ctattgcttc caattcttgc
     6661 gatggctggt gcaggacaag tgggtgcagc attggcatta tggattcgct gcaaaaaaga
     6721 taaagaatta acagaaatga tcaaaggagc tttaccagtc gggatcctag gaatcggcga
     6781 accattgatt tacggagtaa cattacctct aggacgccca tttattactg cttgtatcgg
     6841 tggaggtatc ggcggggcag tgatcggaat gatcggaaat gtcggggcta tcgctattgg
     6901 accttcagga gccgctttga tcccattgat ctctgacggt aaatggttag gctacatcct
     6961 tggattattg gcagcttatg ctggtggctt cgttgctaca ttcttcttcg gtattcctaa
     7021 agaccgttta gaaaaagaag aattagcgac tgaaacagcc acagtaaatg aagtgcctga
     7081 aaaagtagta cctcaaactt cagctgaaag tgtgactatc ctctcatcag ttgctgacgg
     7141 aacagttgtt tctttagata aagcaagtga tccagttttt gcacaaaaga tgatgggtga
     7201 aggctacttt gtagaaccag aaaacggaca tatctattct ccagttactg gggtagtcag
     7261 ctctgtcttc ccaacgaaac acgctattgg gatcacaacg ccaaatggtt tagaaatctt
     7321 gcttcatatg ggaatcaaca cagttgactt aggcggtacg ccatttgatg tgaaagtaac
     7381 agaagggcag caagtgacac ctgatacgct tgtagcagaa gctgacttag aagcgatcaa
     7441 agcagcaggc aaagaaacat ccatgatcgt cttggtaacc aatatggatc gtgtaaaaaa
     7501 ctttgtcttg gaaaaaacag gtaaagcaaa agcaaaagca cctgtgatgg atgtcaaagc
     7561 ttaatatgat agaaacaata gaaacgaaag aagaaactga aatctatttt tcagcttctt
     7621 cttttttatt atttattgtt ttctgctaaa atctgataaa ttcgtgtcac atcagatggt
     7681 gttccccgta attcataatc tgccaagaaa ggaaagccaa atgtttgtgc atattgtttg
     7741 gctgttagac aatactgatt attgaagttt ttatttccgc taccgactac accaagacaa
     7801 agtttatgat tgtcttcatg ttccagatat tcacgcatcg tttcggtcaa gatttctgta
     7861 tcaccgttat ctaaaccatt tc
//
LOCUS       DANKAZ010000295         5613 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.guided.131, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000295 DANKAZ010000000
VERSION     DANKAZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.guided.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            complement(40..1026)
                     /locus_tag="PWY67_003033"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="PWY67_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="PWY67_003034"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="PWY67_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL2059210.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="PWY67_003035"
     CDS             1851..3005
                     /locus_tag="PWY67_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2059211.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="PWY67_003036"
     CDS             3220..4188
                     /locus_tag="PWY67_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL2059212.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="PWY67_003037"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="PWY67_003037"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL2059213.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="PWY67_003038"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="PWY67_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DANKAZ010000296         3227 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.guided.182, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000296 DANKAZ010000000
VERSION     DANKAZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.guided.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            108..1148
                     /locus_tag="PWY67_003039"
     CDS             108..1148
                     /locus_tag="PWY67_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2059214.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1246..1434
                     /locus_tag="PWY67_003040"
     CDS             1246..1434
                     /locus_tag="PWY67_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDL2059215.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1492..1845)
                     /locus_tag="PWY67_003041"
     CDS             complement(1492..1845)
                     /locus_tag="PWY67_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2059216.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2376..2798
                     /locus_tag="PWY67_003042"
     CDS             2376..2798
                     /locus_tag="PWY67_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2059217.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2795..3025
                     /locus_tag="PWY67_003043"
     CDS             2795..3025
                     /locus_tag="PWY67_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2059218.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
BASE COUNT         1073 a          536 c          590 g         1028 t
ORIGIN      
        1 ctccaaaact gaactactta cttgcatata tacgccttta tagtggagta ctacatttac
       61 gagattcggt tagagtatca gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa
      121 taaatggtga attatgtaag attgatagag cttattctgg agaaattgtt attttgcaaa
      181 atgagttttt gaagttaaat agtgttcttg gagatacaaa actattgcca cagagaaaaa
      241 agattgaaaa tccgcaccct ctactacaaa caactgttga accgagtaaa cctgaacaga
      301 gagaaatgtt gcttgatgcc cttttggaaa tctcagatag tgatccgctt ctacgatatt
      361 acgtggattc tacgacacat gaaattatac tttctttctt agggaaagta caaatggaag
      421 tgattagtgc actgttgcaa gaaaagtatc atgtggagat agaactaaaa gagcctacag
      481 tcatttatat ggagagaccg ttaaaaaatg cagaatatac cattcacatc gaagtgccgc
      541 caaatccttt ctgggcttcc attggtttat ctgtatcacc gcttccgttg ggaagtggaa
      601 tgcagtatga gagctcggtt tctcttggat acttaaatca atcatttcaa aatgcagtta
      661 tggaagggat acgctatggt tgcgaacaag gattatatgg ttggaatgtg acggattgta
      721 aaatctgttt taagtacggt ttatactata gccctgttag tactccagca gattttcgga
      781 tgcttactcc tattgtactg gagcaagcct ttagaaaagc tggaacagaa ttgttagagc
      841 catatcttag ttttaaagtt tatgcaccac aggaatatct ttcacgggca tataacgatg
      901 ctcccaaata ttgtgcaaat atcgtaaata ctcaactgaa aaataatgag gtcattatta
      961 ttggagaaat tcctgctcga tgtattcaag attatcgcaa tgatttaact ttttttacaa
     1021 atgggcttag tgtttgttta gcagagctaa aaggatatca ggttaccact ggcgaacctg
     1081 tttgccagac ccgtcgtcta aatagtcgga tagataaagt aagatatatg ttcaataaaa
     1141 taacttagtg cgttttatgt tgttatataa atatggtttc ttattaaata agatgaaata
     1201 ttctttaata tagatttgaa ttaaagtgga aaggaggaga ttgttattat aaactacaag
     1261 tggatattgt gtcctatttg tggaaataaa acaagactac gaatacgagt ggatactata
     1321 cttaaaaatt tccctttata ctgccccaaa tgtaagaacg aaactttaat taatgttcaa
     1381 aaaatgaata taataacaat caaagagcca gacgccaaga cgcagagccg ataatttgag
     1441 aaatgaaact ctcatcttat cggctctttt tgtttatctg aattttactg actagccttc
     1501 aatatttctt gcttcattga taccttttgc tagtgcttcc attaaggata gttctttgtc
     1561 tgtaaagcta tccatgtatt tttctacctg taaccttcga gtgcttttaa tcaaattatc
     1621 tgtaggtaag aaaaattcat caatagatac atggagtaat gatacaagat catacagcac
     1681 ttgtgtactt gggtgttgtc ctttgttttc tatatttgtt aaataacgtg ggtcaatttc
     1741 aatcattgtt cccacttgtt cacgagtgag accccgtttc attcgagcct ctttaatcgc
     1801 taaaccaacg gctctaaaat cgtatttatc ttctttttta cgcatactaa atcacctcta
     1861 tacattttac tgttcctata taaccataaa caggtataga aaaacatgtt atatagttta
     1921 tgagttccta tagaacactg tggaatttat taatgcgatg aatccaattt caaatagaaa
     1981 ggtcgctgat tttatttacc tatacaactt tataaaattt agtcatactc ctagaaagta
     2041 gataaaattg gcgacttaat ggagggaaaa gtcgccatga gaaagataat ctgtcatcta
     2101 gttctcttaa ttaagttctt atcaaaaaaa cttatccacc tgtactggat agatatgacc
     2161 atcaatgtgg ttttgcagat tacatagata gaaaaatcaa agcatatgaa cagagaaaac
     2221 aatctgttta tgtgcttttt tggtcactta ataaatatct tttaggattt ctcttcattt
     2281 atgcaacaaa tcccccgttc gtgtcggggt ttagtatgaa gggagataaa gaagcacatg
     2341 ccactccctt tcacgacaga aagggggtga aaagcatgaa accatcttct tttcaagaaa
     2401 ccatagaaaa tcagtttgac tatatctgca aacaagttat tgaagatgaa cgaaaagatt
     2461 acttcaaaca tctagacaga ctatcagcaa aagaagtctc gttttctgat atagataact
     2521 accttgtcaa ctctttttct actgttgata cgtacgtcac tgatttccaa ctatttaaat
     2581 tatttggatt aagaatcaat gttgaaagtg atttattaag tgaagccctg cgtcatcttt
     2641 cagagaaaaa acggaacatt attttacttc attactttat ggatatgagt gatgtggaga
     2701 ttgcggagtt actaaatttg aatcgttcga ctgtttatcg tcatcgaata agtggactag
     2761 ccatgattaa agaattcatg aaggagtgta aagaatgaaa acagcttatc caagggttcc
     2821 cttccccctc attgtaaagg cgacagatgg cgacgtggaa gcaattaatc agattgttaa
     2881 acattatcga gggtatacct caaaacgttc attacgtcgc atgacagacg aatatggaaa
     2941 ttctcatatg gttattgatg aaaccctacg tggcagaatg gaaacgaaac tcatctctaa
     3001 gattttatcg ttcgaaatta aatagtccca ttttttcgtg gaagcgtgtc gaatcgtatc
     3061 cacgcttccc gatttgggag gtttgttatt ccatcaaagc atattgtgtg ttcagtaggt
     3121 tttgataggc taacaagcca aacgttcttt gacaaccaaa taaaggcaat cgaatacgtt
     3181 tcggtaagaa aagagctgat gaactaacac gccatgacct acaaaat
//
LOCUS       DANKAZ010000297         2627 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.guided.194, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000297 DANKAZ010000000
VERSION     DANKAZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.guided.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            188..685
                     /gene="dfrG"
                     /locus_tag="PWY67_003044"
     CDS             188..685
                     /gene="dfrG"
                     /locus_tag="PWY67_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2059219.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(757..2568)
                     /locus_tag="PWY67_003045"
     CDS             complement(757..2568)
                     /locus_tag="PWY67_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2059220.1"
                     /translation="MLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKP
                     SRYKMTGSSKDKGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDV
                     EPDYQFHKEGCSEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTT
                     YHQQKNTILPMFAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQP
                     LQTKKFNEMWLNTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFR
                     SDVAYDWNISMDELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLH
                     ELGKITNRSRFVDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFT
                     KELRLSDAHHFICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLT
                     ELLTSHQFHEEAISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAK
                     MLLNVNDHSTNSFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTY
                     YNFCRPFKSADKKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          821 a          432 c          467 g          907 t
ORIGIN      
        1 tacaaaatga tttataaaat gcaattgaaa atgataattg aatgaaaatt tgtgattttg
       61 tggtattatg ataatataac ttatagaaaa aattgcttat tcaactaacg ggcaggataa
      121 tgagaaaagg ttttttgata gaaataagaa attagatggt aaattagtta aaaaggctgt
      181 gttataaatg aaagtttctt tgattgctgc gatggataag aatagagtga ttggcaaaga
      241 gaatgacatt ccttggagga ttcccaagga ctgggaatat gttaaaaata ctacaaaggg
      301 acatccgata atattaggta ggaagaacct tgaatcaatc ggaagagcct tacctgacag
      361 aagaaatatt attctgacga gagataaggg gtttaccttt aatggttgtg aaattgttca
      421 ttcaatagaa gatgtttttg agttatgtaa aaacgaagaa gaaattttta ttttcggagg
      481 agaacagatt tataatttgt ttttccctta tgttgagaaa atgtacatca caaaaataca
      541 tcatgaattc gaaggagata ctttttttcc agaagtgaat tatgaggaat ggaatgaggt
      601 atttgcccaa aaagggataa agaatgataa aaatccgtat aactactatt ttcatgtata
      661 tgaaagaaaa aacttattga gttaacgggt gcgttaattg aagaagaaca gtaaagataa
      721 agcacaagag cttgggatta ctccaagctc tttttgtcat ttgaaataaa ttatatcttt
      781 ccaatcaaat tgtttatctg taatccctaa tctttgagca ggtgtcaaaa cctttttatc
      841 cgctgattta aaagggcgac aaaagttgta gtaagttctt agaattgtaa tcgcaaattg
      901 agcatacttc ggattgaagt tagcgtaaat atagctcttc ttatcaccac gagctgttgt
      961 taatggtctt tcaagagatg atatataacg tcttatctgt tgaataaagc tattggtcga
     1021 atggtcatta acatttaata gcatctttgc gatttcgttt ggttccagag cagataaatc
     1081 cgttctacaa tcaactgaat aaaatccttt atccttagtg gctaatggat gtttgatagg
     1141 gttatcggca tatttgcgat atctttctcc atctttacta atagcctctt cgtggaattg
     1201 atgacttgtt agtaattcag ttagataatg agatgccaaa gtccgtagaa actttgtttt
     1261 gaatcctctg atatctcccc aatcgagcag ttctgctttt gcttgtccga attcttttaa
     1321 gcattgtttt ctgcttttcg tcttgttaac ttggcaaata aaatgatgtg catcgctcaa
     1381 ccttaattct ttcgtgaata ctctataaaa ggcgtttcta atagagaagt catcatcact
     1441 aatcattcgc cattcgtcgg cattaaccat ttgtttaatt aaccaatagt gagcagttgt
     1501 agtataagga gcatcaacat ttaaaccatc gacaaatcta ctcctattag taattttccc
     1561 taattcgtga agataagcat tcctattttc gctatcgtta gcggatggtt cttgtggata
     1621 ataagaccaa tctaacctat catttttacg actaaaagta ttgagatggt cttctttgaa
     1681 ttttcttgta tcttcattta attcatccat tgaaatattc caatcatagg caacatcaga
     1741 acggaacaca tatcttgata agacctcagc agatacaact atatacgttt gcatattaag
     1801 gtcttcaaac cccgtatatt tctttgaacc ttgtcctttc ttacggacgt tatttaggta
     1861 gtagtgcatc ttgtccgtat taagccacat ctcgttaaat ttctttgttt gaaggggttg
     1921 tgtctcatat cgttctaaaa actccaaaca acgcctatac agccactcca gcttatgata
     1981 gtaggttcca actccaattc ctaaaatatc acaagtacga ctgactgaaa cccttccaac
     2041 aaccatctta gcgaacattg gaagaatggt gttcttttgt tggtgataag tagtggtttc
     2101 ttctcttttt ggtagaacat tagtgaactt cttacacgcc ttacattgat agcgttgaga
     2161 tttgccccta cttttccccc ttttgtaaaa ttgcttcggc tcattgaatg gggttgattc
     2221 ttcttccgag cagccttctt tatgaaattg ataatctggc tcaacatctt ggatgctatt
     2281 gatttctata aggcgtttaa tttcttcaac aacagaccaa ttagataaag gggttactgt
     2341 acagttttgc gatacacctc tgccaatagg attgtcattg cagtacatac ctttatgacc
     2401 tttatctttg gaactaccag tcattttata tcgacttggt ttccctttta catctttgaa
     2461 tttgtgctgt tctttcccaa aattacaaca aaacggattg atgcagtaat tatattgaat
     2521 gggatattca gaaccgttcc atttaaaaga cgttggcaag aacaacattt tgttatacct
     2581 tgttgcaaat cgttttggag ttaataaatc atattgttta gccctat
//
LOCUS       DANKAZ010000298         1350 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.guided.232, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000298 DANKAZ010000000
VERSION     DANKAZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.guided.232"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            47..298
                     /locus_tag="PWY67_003046"
                     /pseudo
     CDS             47..298
                     /locus_tag="PWY67_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="PWY67_003047"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="PWY67_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL2059221.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DANKAZ010000299         1196 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.guided.241, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000299 DANKAZ010000000
VERSION     DANKAZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.guided.241"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            259..>1196
                     /gene="vanY"
                     /locus_tag="PWY67_003048"
     CDS             259..>1196
                     /gene="vanY"
                     /locus_tag="PWY67_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HDL2059222.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          428 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaataa
//
LOCUS       DANKAZ010000300         1077 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.guided.250, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000300 DANKAZ010000000
VERSION     DANKAZ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.guided.250"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..50
                     /locus_tag="PWY67_003049"
     CDS             <1..50
                     /locus_tag="PWY67_003049"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL2059223.1"
                     /translation="STVLKQTKNSDYADK"
     gene            175..912
                     /gene="erm(B)"
                     /locus_tag="PWY67_003050"
     CDS             175..912
                     /gene="erm(B)"
                     /locus_tag="PWY67_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2059224.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          411 a          181 c          159 g          326 t
ORIGIN      
        1 catctactgt tttgaaacag actaaaaaca gtgattacgc agataaataa atacgttaga
       61 ttaattccta ccagtgacta atcttatgac tttttaaaca gataactaaa attacaaaca
      121 aatcgtttaa cttctgtatt tgtttataga tgtaatcact tcaggagtga taacatgaac
      181 aaaaatataa aatattctca aaacttttta acgagtgaaa aagtactcaa ccaaataata
      241 aaacaattga atttaaaaga aaccgatacc gtttacgaaa ttggaacagg taaagggcat
      301 ttaacgacga aactggctaa aataagtaaa caggtaacgt ctattgaatt agacagtcat
      361 ctattcaact tatcgtcaga aaaattaaaa ctgaatactc gtgtcacttt aattcaccaa
      421 gatattctac agtttcaatt ccctaacaaa cagaggtata aaattgttgg gaatattcct
      481 taccatttaa gcacacaaat tattaaaaaa gtggtttttg aaagccgtgc gtctgacatc
      541 tatctgattg ttgaagaagg attctacaag cgtaccttgg atattcaccg aacactaggg
      601 ttgctcttgc acactcaagt ctcgattcag caattgctta agctgccagc ggaatgcttt
      661 catcctaaac caaaagtaaa cagtgtctta ataaaactta cccgccatac cacagatgtt
      721 ccagataaat attggaagct atatacgtac tttgtttcaa aatgggtcaa tcgagaatat
      781 cgtcaactgt ttactaaaaa tcagtttcat caagcaatga aacacgccaa agtaaacaat
      841 ttaagtaccg ttacttatga gcaagtattg tctattttta atagttatct attatttaac
      901 gggaggaaat aattctatga gtcgcttttg taaatttgga aagttacacg ttactaaagg
      961 gaatgtagat aaattattag gtatagggca cctctaataa ctaccaatta attcacctca
     1021 aaatgaaatt agaaaaaaac agcgaaattt ttaaatctat ttcttatcga tacaaat
//
LOCUS       DANKAZ010000301          705 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538814-rid17233763.guided.281, whole genome shotgun
            sequence.
ACCESSION   DANKAZ010000301 DANKAZ010000000
VERSION     DANKAZ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538814
            Sequence Read Archive: ERR10688377
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638138.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638138.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 18:33:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,051
            CDSs (total)                      :: 2,984
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 39 of 76
            Pseudo Genes (internal stop)      :: 20 of 76
            Pseudo Genes (multiple problems)  :: 6 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538814-rid17233763.guided.281"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-04-13"
     gene            <1..634
                     /locus_tag="PWY67_003051"
     CDS             <1..634
                     /locus_tag="PWY67_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HDL2059225.1"
                     /translation="SILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLD
                     YTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLC
                     HNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMY
                     GNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          298 a           51 c          120 g          236 t
ORIGIN      
        1 atctatacta ggttataaag aaattaaagg aactttttta acaccagaaa tttattctac
       61 tatgtcagaa gaagaacaaa atttgttaaa acgagatatt gccagttttt taagacaaat
      121 gcacggttta gattatacag atattagtga atgtactatt gataataaac aaaatgtatt
      181 agaagagtat atattgttgc gtgaaactat ttataatgat ttaactgata tagaaaaaga
      241 ttatatagaa agttttatgg aaagactaaa tgcaacaaca gtttttgagg gtaaaaagtg
      301 tttatgccat aatgatttta gttgtaatca tctattgtta gatggcaata atagattaac
      361 tggaataatt gattttggag attctggaat tatagatgaa tattgtgatt ttatatactt
      421 acttgaagat agtgaagaag aaataggaac aaattttgga gaagatatat taagaatgta
      481 tggaaatata gatattgaga aagcaaaaga atatcaagat atagttgaag aatattatcc
      541 tattgaaact attgtttatg gaattaaaaa tattaaacag gaatttatcg aaaatggtag
      601 aaaagaaatt tataaaagga cttataaaga ttgattatat aatatatgaa aagctattat
      661 aaaagacggt tctgttgcaa agttttaaat ctactatcaa ataag
//
